title: e2g: an interactive web-based server for efficiently mapping large EST and cDNA sets to genomic sequences.
abstract: e2g is a web-based server which efficiently maps large expressed sequence tag (EST) and cDNA datasets to genomic DNA. It significantly extends the volume of data that can be mapped in reasonable time, and makes this improved efficiency available as a web service. Our server hosts large collections of EST sequences (e.g. 4.1 million mouse ESTs of 1.87 Gb) in precomputed indexed data structures for efficient sequence comparison. The user can upload a genomic DNA sequence of interest and rapidly compare this to the complete collection of ESTs on the server. This delivers a mapping of the ESTs on the genomic DNA. The e2g web interface provides a graphical overview of the mapping. Alignments of the mapped EST regions with parts of the genomic sequence are visualized. Zooming functions allow the user to interactively explore the results. Mapped sequences can be downloaded for further analysis. e2g is available on the Bielefeld University Bioinformatics Server at http://bibiserv.techfak.uni-bielefeld.de/e2g/.
authors.affiliation: Technische Fakultät, Universität Bielefeld, D-33594 Bielefeld, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Krüger
authors.forename: Alexander
authors.initials: A
authors.lastname: Sczyrba
authors.forename: Stefan
authors.initials: S
authors.lastname: Kurtz
authors.forename: Robert
authors.initials: R
authors.lastname: Giegerich
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003196:Computer Graphics; D018076:DNA, Complementary / Q000737:chemistry*; D020224:Expressed Sequence Tags / Q000737:chemistry*; D023281:Genomics / Q000379:methods*; D020407:Internet; D016415:Sequence Alignment; D012984:Software; D014584:User-Computer Interface
chemical_list: D018076:DNA, Complementary