title: Fetal gender determination using real-time quantitative polymerase chain reaction analysis of maternal plasma.
abstract: OBJECTIVE
  Cell-free fetal DNA in maternal blood is a promising non-invasive alternative for prenatal diagnosis. The goal of this study was to determine fetal sex with maternal plasma in pregnant women without previous male offspring.
  
  
  METHODS
  Blood samples (3 mL) were collected at 6 to 16 weeks' gestation from 56 healthy pregnant women without previous male offspring. There were 23 pregnancies with male fetuses and 33 pregnancies with female fetuses. DNA was extracted from plasma and qualitative analysis of the sex-determining region Y (SRY) gene sequence on the Y chromosome (SRY-3317T) with dual-labeled fluorescent TaqMan probe using real-time quantitative polymerase chain reaction (PCR) was performed.
  
  
  RESULTS
  In the 56 successfully assayed samples, SRY-positive signals were detected in 20 samples of the 23 pregnancies with male fetuses. None of the 33 successfully assayed samples from women bearing female fetuses yielded a positive signal.
  
  
  CONCLUSIONS
  Because of its high correct prediction rate, fetal gender determination with maternal plasma using real-time quantitative PCR can be used as a pre-test to avoid unnecessary chorionic villus sampling for pregnancies at risk of fetal sex-associated disorders.
authors.affiliation: Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, No. 7 Chung-Shan S. Road, Taipei 100, Taiwan.
authors.forename: Hsiao-Lin
authors.initials: HL
authors.lastname: Hwa
authors.forename: Tsang-Ming
authors.initials: TM
authors.lastname: Ko
authors.forename: Men-Luh
authors.initials: ML
authors.lastname: Yen
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Chiang
mesh_terms: D000328:Adult; D041322:Chromosomes, Human, Y / Q000235:genetics; D004247:DNA / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D004247:DNA