title: Gluten of spelt wheat (Triticum aestivum subspecies spelta) as a source of peptides promoting viability and product yield of mouse hybridoma cell cultures.
abstract: The enzymic hydrolysate of gluten from spelt wheat (Triticum aestivum subsp. spelta), an ancient protein-rich wheat subspecies, was subjected to repeated chromatography runs on the small pore size exclusion chromatography matrix, Biogel P-2. Two small peptide fractions were purified by rechromatography. The amino acid analyses carried out upon total hydrolysis of these fractions have shown a very high proportion of glutamic acid/glutamine, leucine, and methionine. The biological activity of the peptide fractions was tested on a model hybridoma at a concentration range from 0.02 to 0.2%. The most striking effect of peptide fractions, apparent even at the lowest concentrations tested, was a significantly higher persistence of viable cells on day 6, i.e., at the decline phase of the cultures. Culture viability values in the presence of peptide fractions were 64-74%, in comparison with 56% in the control culture. The results of this work are consistent with the concept that peptide molecules may act as antiapoptotic agents, survival factors, rather than serving as metabolic substrates.
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Radiova 1, CZ-10227 Prague 10, Czech Republic. franek@biomed.cas.cz
authors.forename: Frantisek
authors.initials: F
authors.lastname: Franek
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018698:Glutamic Acid / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D005983:Glutens / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects*; D006868:Hydrolysis; D007930:Leucine / Q000032:analysis; D008715:Methionine / Q000032:analysis; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis / Q000494:pharmacology*; D014908:Triticum / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D005973:Glutamine; D018698:Glutamic Acid; D005983:Glutens; D008715:Methionine; D007930:Leucine