Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia.
In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyrus. NF-150 immunoreactivity and protein contents of NF-150 and RT 97 (polyphosphorylation epitopes of neurofilament) were significantly decreased at 15 min after ischemic insult. Between 30 min and 12 h after ischemic insult, NF-150 immunoreactivity and protein content were significantly increased as compared with the sham-operated group. Thereafter, NF-150 immunoreactivity and protein content started to decrease. At 12 h after ischemic insult, unlike dentate gyrus, NF-150 immunoreactivity increased in pyramidal cells of the CA1 region. Thereafter, NF-150 immunoreactivity in the CA1 region started to decrease, and 4 days after ischemic insult, NF-150 immunoreactivity nearly was similar to that of the sham-operated group. These biphasic patterns of NF-150 immunoreactivity in the hippocampus and dentate gyrus are reverse correlated with that of the intracellular calcium influx. For calcium detection in the CA1 region, we also conducted alizarin red staining. Alizarin red positive neurons were detected in some neurons at 15-30 min after ischemic insult. At 12 h after ischemia, alizarin red positive neurons were decreased. Thereafter, alizarin red positive neurons started to decrease, but alizarin positive neurons were significantly increased in dying neurons 4 days after ischemia. These results suggest that ischemia-related changes of NF-150 expression may be caused by the calcium following transient forebrain ischemia.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine, Hallym University, Okchon-dong 1, Chunchon 200-702, South Korea.
Do
Seon-Gil
SG
Yoo
Ki-Yeon
KY
Kim
Duk Soo
DS
Cho
Jun Hwi
JH
Kwon
Young-Guen
YG
Lee
Jae-Yong
JY
Oh
Yang-Seok
YS
Kang
Tae-Cheon
TC
Won
Moo Ho
MH
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002118:Calcium / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D018891:Dentate Gyrus / Q000378:metabolism*; D005849:Gerbillinae; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism; D013995:Time; D013997:Time Factors
D016900:Neurofilament Proteins; C050934:neurofilament protein 150; D002118:Calcium