title: Development of an automated sample preparation module for environmental monitoring of biowarfare agents.
abstract: An automated sample preparation module, based upon sequential injection analysis (SIA), has been developed for use within an autonomous pathogen detection system. The SIA system interfaced aerosol sampling with multiplexed microsphere immunoassay-flow cytometric detection. Metering and sequestering of microspheres using SIA was found to be reproducible and reliable, over 24-h periods of autonomous operation. Four inbuilt immunoassay controls showed excellent immunoassay and system stability over five days of unattended continuous operation. Titration curves for two biological warfare agents, Bacillus anthracis and Yersinia pestis, obtained using the automated SIA procedure were shown to be similar to those generated using a manual microtiter plate procedure.
authors.affiliation: Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94551, USA.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Hindson
authors.forename: Steve B
authors.initials: SB
authors.lastname: Brown
authors.forename: Graham D
authors.initials: GD
authors.lastname: Marshall
authors.forename: Mary T
authors.initials: MT
authors.lastname: McBride
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Makarewicz
authors.forename: Dora M
authors.initials: DM
authors.lastname: Gutierrez
authors.forename: Duane K
authors.initials: DK
authors.lastname: Wolcott
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Metz
authors.forename: Ramakrishna S
authors.initials: RS
authors.lastname: Madabhushi
authors.forename: John M
authors.initials: JM
authors.lastname: Dzenitis
authors.forename: Billy W
authors.initials: BW
authors.lastname: Colston
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D001408:Bacillus anthracis / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D001694:Biological Warfare; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D013997:Time Factors; D015010:Yersinia pestis / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity
chemical_list: D000907:Antibodies, Bacterial