Oral immunization with an rfaH mutant elicits protection against salmonellosis in mice.
Loss of the transcriptional antiterminator RfaH results in virulence attenuation (>10(4)-fold increase in 50% lethal dose) of the archetypal Salmonella enterica serovar Typhimurium strain SL1344 by both orogastric and intraperitoneal routes of infection in BALB/c mice. Oral immunization with the mutant efficiently protects mice against a subsequent oral infection with the wild-type strain. Interestingly, in vitro immunoreactivity is not confined to strain SL1344; rather, it is directed also towards other serovars of S. enterica and even Salmonella bongori strains.
Nagy
Gábor
G
Institute of Medical Microbiology and Immunology, University of Pécs, Szigeti út 12, 7624 Pécs, Hungary.
Dobrindt
Ulrich
U
Hacker
Jörg
J
Emödy
Levente
L
D000284:Administration, Oral; D000818:Animals; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009154:Mutation; D010445:Peptide Elongation Factors / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*; D015534:Trans-Activators / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014774:Virulence
D029968:Escherichia coli Proteins; D010445:Peptide Elongation Factors; C106401:RfaH protein, E coli; D022562:Salmonella Vaccines; D015534:Trans-Activators