Reliability of the detection of meningococcal gamma-glutamyl transpeptidase as an identification marker for Neisseria meningitidis.
Detection of gamma-glutamyl transpeptidase (GGT; ggt ) activity is one of the useful methods for a specific identification of Neisseria meningitidis. However, we previously happened to isolate a ggt -deficient N. meningitidis strain (NIID113) from a healthy carrier. In this study, in order to re-examine the reliability of the marker, we again investigated the GGT activity of 245 N. meningitidis human isolates and identified two other GGT-defective N. meningitidis isolates besides NIID113. The isolation frequency (1.2%) of ggt mutants among human isolates strongly confirmed the 98.8% reliability of GGT activity as the identification marker for N. meningitidis.
Takahashi
Hideyuki
H
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Kuroki
Toshiro
T
Watanabe
Yuko
Y
Dejsirilert
Surang
S
Saengsuk
Leelaowadee
L
Yamai
Shiro
S
Watanabe
Haruo
H
D000328:Adult; D002353:Carrier State / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D007564:Japan; D008297:Male; D008589:Meningococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009345:Neisseria meningitidis / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013045:Species Specificity; D013785:Thailand; D005723:gamma-Glutamyltransferase / Q000032:analysis / Q000235:genetics*
D005723:gamma-Glutamyltransferase