Chitosan sponges as tissue engineering scaffolds for bone formation.
Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.
Seol
Yang-Jo
YJ
Department of Periodontology, College of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul 110-749, South Korea.
Lee
Jue-Yeon
JY
Park
Yoon-Jeong
YJ
Lee
Yong-Moo
YM
Young-Ku
Rhyu
In-Chul
IC
Lee
Seung-Jin
SJ
Han
Soo-Boo
SB
Chung
Chong-Pyoung
CP
D000818:Animals; D016723:Bone Remodeling; D002478:Cells, Cultured; D048271:Chitosan; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D012886:Skull / Q000166:cytology; D023822:Tissue Engineering
D048271:Chitosan