Characterization of single stranded telomeric DNA-binding proteins in cultured soybean (Glycine max) cells.
We have identified and characterized a protein factor in soybean (Glycine max) nuclear extracts that binds to plant single stranded telomeric DNA repeats. A single DNA-protein complex was detected in gel retardation assays using synthetic telomeres and nuclear extracts. The protein forming this complex was designated soy-bean (Glycine max) single stranded telomeric DNA-binding protein (Gm-STBP). Gm-STBP binds to single stranded telomeric DNA containing more than two repeats. It does not bind to Tetrahymena, human or mutated plant telomere sequences, and its binding activity is not affected by RNase treatment. Gm-STBP activity gradually decreased after suspension cultures entered stationary phase. A slower migrating band was formed with extracts of earlier and later phases of soybean suspension cultures. Our findings suggest that binding of Gm-STBP to plant single stranded telomeric DNA may play a role in the proper functioning of telomeres during development.
Kwon
Chian
C
Department of Biology, Yonsei University, Seoul 120-749, Korea.
Kwon
Kisang
K
Chung
In Kwon
IK
Kim
Soon Young
SY
Cho
Myeon Haeng
MH
Kang
Bin Goo
BG
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism*; D034501:Telomere-Binding Proteins / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D034501:Telomere-Binding Proteins