[Substances affecting gap junction conductivity and Mauthner neuron mixed synapses].
The aim of this work was to study the effect of dopamine, 20-hydroxyecdysone and chlorpromazine, drugs changing the conductance of gap junctions (GJs), on the ultrastructure of goldfish Mauthner neurons mixed synapses. It was shown that desmosome-like contacts (DLCs) were the presumable targets for dopamine, that increased the electrotonic conductance of mixed synapses. Their hypertrophy, as well as an increase in the number of bridges in their clefts suggests that neuronal actin is involved in the mechanism of dopamine-induced increase of electrotonic conductance. This assumption is further supported by the transformation of extracted monomeric muscular actin into polymeric actin in the presence of dopamine. On the contrary, GJs were shown to be damaged by dopamine treatment. Ecdysone, which is also known to increase the conductance of GJs, changed their structure, increasing their number in the zone of apposition and reducing the profile length, but practically not affecting the DLC structure. It also does not interact with isolated actin in vitro. Therefore, the mechanism of ecdysone action is mainly associated with GJ function. Chlorpromazine, which reduces the conductance of GJs, was shown to damage, partially or completely, DLC admembranous fibrillar material, thus preventing actin polymerization, as shown by in vitro experiments, but had no effect on GJs. Therefore, the mechanism of its action, appears to be based on the changes in the state of neuronal actin.
Pavlik
L L
LL
Laboratory of Neuron Ultrastructure, Pushchino State University.
Bezgina
E N
EN
Tiras
N P
NP
Mikheeva
I V
IV
Udal'tsov
S N
SN
Moshkov
D A
DA
D000199:Actins / Q000378:metabolism; D000818:Animals; D002746:Chlorpromazine / Q000494:pharmacology; D003896:Desmosomes / Q000187:drug effects / Q000648:ultrastructure*; D004298:Dopamine / Q000494:pharmacology; D004440:Ecdysone / Q000494:pharmacology; D017629:Gap Junctions / Q000187:drug effects / Q000648:ultrastructure*; D006054:Goldfish / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure*; D013569:Synapses / Q000187:drug effects / Q000648:ultrastructure*
D000199:Actins; D004440:Ecdysone; D002746:Chlorpromazine; D004298:Dopamine