[Development and morphofunctional characterization of the osteoblastic phenotype in cell culture in vitro].
The objective of this research was to study osteogenic properties of cultured rabbit bone marrow stromal cells, newborn rat cranium bone cells and rat osteocarcoma ROS 17-2/8 cells. For this purpose cytochemical reaction for alkaline phosphatase was performed by the Lowry method, mineral deposition was assessed by staining of the cultures after von Kossa. Cranium bone cells were shown to synthesize alkaline phosphatase (34 +/- 7 nmol/min/10(6) cells), the matrix mineralization being found. Bone marrow stromal cells displayed a lower activity alkaline phosphatase level than did cranium bone cells (4 +/- 0.6 nmol/min/10(6) cells). However, cell cultivation in the presence of dexamethasone in the medium (10(-8) M) induced a higher activity of alkaline phosphatase (9 +/- 1 nmol/min/10(6) cells), mineralization of the extracellular matrix being the case. The highest level of alkaline phosphatase activity was found for ROS 17-2/8 cells (60 +/- 12 nmol/min/10(6) cells) but no matrix mineralization was determined. According to these data, matrix calcification and formation of bone-like nodules are the most important properties of osteoblastic differentiation in vitro.
Deev
R V
RV
Russian Military Medical Academy.
Nikolaenko
N S
NS
Tsupkina
N V
NV
Gololobov
V G
VG
Patokin
I L
IL
Pinaev
G P
GP
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells / Q000201:enzymology / Q000378:metabolism*; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D003907:Dexamethasone; D010006:Osteoblasts / Q000201:enzymology / Q000378:metabolism*; D012516:Osteosarcoma; D011817:Rabbits; D051381:Rats; D012886:Skull / Q000166:cytology*; D013194:Staining and Labeling; D017154:Stromal Cells / Q000378:metabolism
D003470:Culture Media; D003907:Dexamethasone; D000469:Alkaline Phosphatase