Characterization of degradation process of cyanobacterial hepatotoxins by a gram-negative aerobic bacterium.
A bacterium termed 7CY, capable of decomposing cyanobacterial toxins, was isolated from surface water sample of Lake Suwa and degradation of microcystin-RR and nodularin-Har was investigated. The isolated 7CY was a gram-negative, aerobic bacillus, and a member of a genus Sphingomonas. The strain degraded microcystin-LY, -LW, and -LF completely as well as microcystin-LR within 4 days after their addition (6 microg/ml) whereas degradation of nodularin-Har did not occur at all during experiment. On the contrary, the strain was capable of degrading nodularin-Har in the presence of microcystin-RR and both toxins were completely decomposed within 6 days. The strain scarcely degraded nodularin-Har in the presence of microcystin-RR when glucose and ammonium chloride were added to the medium. The degradation of nodularin-Har did not occur in the medium from which bacterial cells had been removed after degradation of microcystin-RR. Furthermore, when microcystin-RR and nodularin-Har were added to the cytoplasm fraction of 7CY cells, microcystin-RR was rapidly degraded within 18 h, but nodularin-Har was not. The strain 7CY may require an enzyme(s) induced during the degradation of microcystin-RR in order to utilize nodularin-Har as nutrition.
Ishii
Hiroshi
H
School of Marine Science and Technology, Tokai University, Shimizu-Orido 3-20-1, Shizuoka 424-8610, Japan.
Nishijima
Miyuki
M
Abe
Toshihiko
T
D001673:Biodegradation, Environmental; D000458:Cyanobacteria / Q000472:pathogenicity*; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000378:metabolism*; D020578:Sphingomonas / Q000502:physiology*; D014871:Water Microbiology
D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C084459:microcystin LY; C063855:microcystin RR