Comparison of two mononuclear cell program settings on two apheresis devices intended to collect high yields of CD14+ and CD3+ cells.
BACKGROUND
In cancer and transplantation therapy apheresis devices and software of optimum standards are required for the collection of high cell yields with high purity of the desired cell fraction.


METHODS
In a paired study, 15 healthy blood donors underwent four 10-L leukapheresis procedures (197 +/- 33 min) with an inlet blood flow rate of 60 mL per minute by use of two different MNC program settings of the COBE Spectra (Gambro BCT) and the AS.TEC 204 (Fresenius Hemocare) cell separators.


RESULTS
Use of the standard MNC program of both apheresis devices resulted in significantly higher (p < 0.01) collection efficiencies of CD14+ monocytes, CD3+ cells, CD4+ cells, CD8+ T cells, CD16+ CD56+ natural killer (NK) cells, and residual PLTs (p < 0.001), owing to higher centrifuge speed. The mean MNC purity of all components was more than 90 percent. By use of standard programs of either device, significant correlations (p < 0.01) between donor monocytes and preleukapheresis NK cell counts and the corresponding component cell yields were found.


CONCLUSIONS
Compared to the program modifications with lower centrifuge velocities the standard MNC programs were significantly more efficient regarding CD14+, CD3+, and CD16+ CD56+ cells. Enhanced centrifuge speed and inlet blood flow rate in MNC programs resulted in higher, similar composed MNC concentrations of the products.
Strasser
Erwin F
EF
Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg, Germany.
Dittrich
Sandra
S
Weisbach
Volker
V
Zimmermann
Robert
R
Ringwald
Jürgen
J
Achenbach
Susanne
S
Zingsem
Jürgen
J
Eckstein
Reinhold
R
D000328:Adult; D001772:Blood Cell Count; D017252:CD3 Complex / Q000032:analysis*; D006400:Hematocrit; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D011446:Prospective Studies; D016176:T-Lymphocyte Subsets / Q000166:cytology*
D017252:CD3 Complex; D018950:Lipopolysaccharide Receptors