Expression of a C terminally truncated form of pertussis toxin S1 subunit effectively induces protection against pertussis toxin following DNA-based immunization.
Four plasmids encoding different C terminally and N terminally truncated pertussis toxin S1 subunits of Bordetella pertussis were constructed and tested for inducibility of protection against pertussis toxin in mice after DNA-based immunization. The region encoding an N-terminal 180-amino-acid fragment of the S1 subunit had the most potent ability to induce protective immunity.
Kamachi
Kazunari
K
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayma-shi, Tokyo 208-0011, Japan. kamachi@nih.go.jp
Arakawa
Yoshichika
Y
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D051379:Mice; D037342:Pertussis Toxin / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D010567:Pertussis Vaccine / Q000276:immunology*; D010957:Plasmids / Q000276:immunology; D014121:Toxoids / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D010567:Pertussis Vaccine; D014121:Toxoids; D019444:Vaccines, DNA; C083388:pertussis toxin, S1 subunit; C051093:pertussis toxoid; D037342:Pertussis Toxin