Plasmids of Staphylococcus cohnii isolated from the intensive-care unit.
Numerous isolates of both subspecies of Staphylococcus cohnii were found in the environment of the intensive-care unit of a pediatric hospital. These isolates carried in their cells many plasmids, up to fourteen, of a wide range of sizes (< 2 to > 56 kb). Striking was the occurrence of large plasmids not very common in staphylococci. These were present in > 80% of S. cohnii isolates. Fifty-two different plasmid profiles were found in 79 investigated isolates belonging to S. cohnii ssp. cohnii and S. cohnii ssp. urealyticus. Isolates similar in plasmid profiles were grouped in antibiotic-resistance clusters established for 9 antibiotics (gentamicin, ciprofloxacin, clindamycin, erythromycin, tetracycline, chloramphenicol, mupirocin, trimethoprim-sulfamethoxazole, vancomycin) using the method of unweighted pair group mathematical averages (UPGMA). Many isolates were multiresistant to antibiotics and produced bacteriocins.
Szewczyk
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EM
Department of Pharmaceutical Microbiology, Medical University of Łódź, 90-235 Łódź, Poland. eligia@uni.lodz.pl
Rózalska
M
M
Cieślikowski
T
T
Nowak
T
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006776:Hospitals, Pediatric; D007362:Intensive Care Units; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016712:Mupirocin / Q000494:pharmacology; D010957:Plasmids; D011044:Poland; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D013752:Tetracycline / Q000494:pharmacology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D005839:Gentamicins; D002981:Clindamycin; D002939:Ciprofloxacin; D004917:Erythromycin; D002701:Chloramphenicol; D014640:Vancomycin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D016712:Mupirocin; D013752:Tetracycline