In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay.
Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC(50)]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC(50) of 0.5 to 1.0 mg/liter).
Ahmed
Abdalla O A
AO
Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
van de Sande
Wendy W J
WW
van Vianen
Wim
W
van Belkum
Alex
A
Fahal
Ahmed H
AH
Verbrugh
Henri A
HA
Bakker-Woudenberg
Irma A J M
IA
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D003124:Colorimetry; D017964:Itraconazole / Q000494:pharmacology*; D020461:Madurella / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008271:Mycetoma / Q000382:microbiology; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results; D013778:Tetrazolium Salts
D000935:Antifungal Agents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D017964:Itraconazole; D000666:Amphotericin B