Specific polyclonal-based immunoassays for sulfathiazole.
A highly sensitive and specific enzyme-linked immunosorbent assay has been developed for detection of sulfathiazole (STZ, 4-amino- N-thiazol-2-yl-benzenesulfonamide). A set of haptens was synthesized in order to produce polyclonal antibodies against sulfonamides. Two ELISA formats (antibody-coated and conjugate-coated) were also investigated using all the serum/coating conjugate combinations that showed specific recognition. The developed ELISA succeeded in detection of STZ at concentrations as low as 0.03 ng mL(-1) over a measurable range of 0.12-6.71 ng mL(-1). Selectivity studies have demonstrated that other sulfonamides do not interfere significantly (<10%) with analysis of STZ by this immunochemical technique. Analysis of spiked bee honey samples by the developed ELISA method showed recoveries were good. The selectivity and sensitivity (IC(50)=1.6 ng mL(-1)) make it a suitable screening method for determination of low levels of STZ in food samples.
Pastor-Navarro
Nuria
N
Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.
García-Bover
Cristina
C
Maquieira
Angel
A
Puchades
Rosa
R
D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D006722:Honey / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000032:analysis*
D000906:Antibodies; D006241:Haptens; D013432:Sulfathiazoles; D000077589:Sulfathiazole