CNS grafts of rat choroid plexus protect against cerebral ischemia in adult rats.
The present study examined the neuroprotective effects of choroid plexus isolated from adult rats and encapsulated within alginate microcapsules. In vitro, conditioned media from cultured choroid plexus produced a marked, dose-dependent protection of embryonic cortical neurons against serum deprivation-induced cell death. In vivo studies demonstrated that a one-hour middle cerebral artery occlusion in adult Wistar rats produced profound motor and neurological impairments 1-3 days after stroke. In contrast, stroke animals transplanted with encapsulated choroid plexus cells displayed a significant reduction in both motor and neurological abnormalities. Histological analysis 3 days post-transplantation revealed that choroid plexus transplants significantly decreased the volume of striatal infarction. This is the first report demonstrating the therapeutic potential of transplanted choroid plexus for stroke.
Borlongan
C V
CV
Department of Neurology/Institute of Molecular Medicine and Genetics/School of Graduate Studies, BI-3080, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912-3200, USA. cborlongan@mail.mcg.edu
Skinner
S J M
SJ
Geaney
M
M
Vasconcellos
A V
AV
Elliott
R B
RB
Emerich
D F
DF
D000704:Analysis of Variance; D000818:Animals; D001519:Behavior; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D016380:Brain Tissue Transplantation / Q000379:methods*; D002214:Capsules / Q000627:therapeutic use; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D002544:Cerebral Infarction / Q000473:pathology / Q000601:surgery; D002831:Choroid Plexus / Q000166:cytology* / Q000502:physiology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D006085:Graft Survival / Q000502:physiology; D008124:Locomotion / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts; D013997:Time Factors
D002214:Capsules; D017077:Culture Media, Conditioned; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium