Immunological and hematological effects observed in B6C3F1 mice exposed to JP-8 jet fuel for 14 days.
JP-8 is the primary jet fuel used by the U.S. Air Force and NATO allies. Exposure is likely to be widespread and to include both military and aviation industry personnel as well as residents living near fuel contaminated sites. This study examines the effects of JP-8 on humoral and cell-mediated and hematological parameters. A suite of immunotoxicological endpoints was evaluated in adult female B6C3F1 mice gavaged with JP-8 (in an olive oil carrier) ranging from 250-2500 mg/kg/d for 14 d. One day following the last exposure, significant increases in liver mass were detected beginning at exposure levels of 1000 mg/kg/d, while thymic mass was decreased at exposure levels of 1500 mg/kg/d and above. Decreases in thymic cellularity, however, were only observed at exposure levels of 2000 mg/kg/d and above. Mean corpuscular volume was increased (1500-2500 mg/kg/d), while the hematocrit, hemoglobin concentration, and red blood cell count were decreased only at the 2500 mg/kg/d exposure level. Natural killer cell (NK) activity and T- and B-cell proliferation were not altered. Decreases in the plaque-forming cell (PFC) response were dose responsive at levels of 500 mg/kg/d and greater, while unexpectedly, serum levels of anti-SRBC immunoglobulin M (IgM) were not altered. Alterations were detected in thymic and splenic CD4/8 subpopulations, and proliferative responses of bone marrow progenitor cells were enhanced in mice exposed to 2000 mg/kg/d of JP-8. This study establishes that humoral immune function is impaired with lower exposure levels of JP-8 than are required to affect primary and secondary immune organ weights and cellularities, CD4/8 subpopulations, and hematological endpoints.
Keil
D
D
National Institute of Occupational Safety and Health, Morgantown, West Virginia, and Department of Health Professions, Medical University of South Carolina, Charleston, South Carolina, USA. dkeil@cdc.gov
Dudley
A
A
EuDaly
J
J
Dempsey
J
J
Butterworth
L
L
Gilkeson
G
G
Peden-Adams
M
M
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001769:Blood / Q000187:drug effects*; D016516:CD4-CD8 Ratio; D004195:Disease Models, Animal; D004781:Environmental Exposure / Q000009:adverse effects* / Q000032:analysis; D004784:Environmental Monitoring; D004906:Erythrocyte Count; D004909:Erythrocyte Indices / Q000187:drug effects; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects; D006838:Hydrocarbons / Q000737:chemistry / Q000506:poisoning*; D006965:Hyperplasia; D007111:Immunity, Cellular / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013723:Teratogens; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology
D006454:Hemoglobins; D006838:Hydrocarbons; C098607:JP8 aviation fuel; D013723:Teratogens