Fate and toxic effects of inhaled ultrafine cadmium oxide particles in the rat lung.
Female Fischer 344 rats were exposed to ultrafine cadmium oxide particles, generated by spark discharging, for 6 h at a concentration of 70 microg Cd/m(3) (1 x 10(6)/cm(3)) (40 nm modal diameter). Lung morphology and quantification of Cd content/concentration by inductively coupled plasma (ICP)-mass spectrometry were performed on days 0, 1, 4, and 7 after exposure. Cd content in the lung on day 0 was 0.53 +/- 0.12 microg/lung, corresponding to 19% of the estimated total inhaled cumulative dose, and the amount remained constant throughout the study. In the liver no significant increase of Cd content was found up to 4 days. A slight but statistically significant increase was observed in the liver on day 7. We found neither exposure-related morphological changes of lungs nor inflammatory responses in lavaged cells. Another group of rats were exposed to a higher concentration of ultrafine CdO particles (550 microg Cd/m(3) for 6 h, 51 nm modal diameter). The rats were sacrificed immediately and 1 day after exposure. The lavage study performed on day 0 showed an increase in the percentage of neutrophils. Multifocal alveolar inflammation was seen histologically on day 0 and day 1. Although the Cd content in the lung was comparable between day 0 and day 1 (3.9 microg/lung), significant elevation of Cd levels in the liver and kidneys was observed on both days. Two of 4 rats examined on day 0 showed elevation of blood cadmium, indicating systemic translocation of a fraction of deposited Cd from the lung in this group. These results and comparison with reported data using fine CdO particles indicate that inhalation of ultrafine CdO particles results in efficient deposition in the rat lung. With regard to the deposition dose, adverse health effects of ultrafine CdO and fine CdO appear to be comparable. Apparent systemic translocation of Cd took place only in animals exposed to a high concentration that induced lung injury.
Takenaka
S
S
GSF-National Center for Environment and Health, Institute for Inhalation Biology, Neuherberg/Munich, Germany. takenaka@gsf.de
Karg
E
E
Kreyling
W G
WG
Lentner
B
B
Schulz
H
H
Ziesenis
A
A
Schramel
P
P
Heyder
J
J
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002104:Cadmium / Q000032:analysis / Q000097:blood; D019187:Cadmium Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D005260:Female; D007249:Inflammation; D019570:Inhalation Exposure; D007668:Kidney / Q000737:chemistry / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008168:Lung / Q000737:chemistry / Q000473:pathology*; D009504:Neutrophils / Q000276:immunology; D010087:Oxides / Q000493:pharmacokinetics* / Q000633:toxicity*; D010316:Particle Size; D051381:Rats; D011916:Rats, Inbred F344; D014028:Tobacco Smoke Pollution / Q000032:analysis
D019187:Cadmium Compounds; D010087:Oxides; D014028:Tobacco Smoke Pollution; D002104:Cadmium; C029663:cadmium oxide