Simian virus 40 infection down-regulates the expression of nitric oxide synthase in human mesothelial cells.
The cytotoxic effects of asbestos are partly mediated by the production of free radicals, including nitric oxide (NO). SV40 has been suggested to synergize with asbestos in the pathogenesis of malignant mesothelioma. Crocidolite asbestos fibers induced in human mesothelial and malignant mesothelioma cells a significant increase of NO synthase activity and expression, which was absent in SV40-infected cells. Furthermore, SV40 infection prevented the NF kappa B activation elicited by crocidolite in both mesothelial and mesothelioma cells. These data suggest that SV40, by inhibiting the synthesis of NO, could favor the survival of transformed, potentially neoplastic cells.
Aldieri
Elisabetta
E
Department of Genetics, Biology and Biochemistry, University of Torino and Research Center on Experimental Medicine, Turin, Italy.
Orecchia
Sara
S
Ghigo
Dario
D
Bergandi
Loredana
L
Riganti
Chiara
C
Fubini
Bice
B
Betta
Pier Giacomo
PG
Bosia
Amalia
A
D017638:Asbestos, Crocidolite / Q000009:adverse effects; D002478:Cells, Cultured; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology* / Q000821:virology*; D006801:Humans; D008654:Mesothelioma / Q000201:enzymology* / Q000209:etiology / Q000821:virology; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D027601:Polyomavirus Infections / Q000201:enzymology / Q000821:virology; D013539:Simian virus 40 / Q000502:physiology*; D014412:Tumor Virus Infections / Q000201:enzymology / Q000821:virology
D016328:NF-kappa B; D017638:Asbestos, Crocidolite; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II