Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography.
High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%.
Chen
Feng
F
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China. sfchen@hkusua.hku.hk
Lu
Hai-Tao
HT
Jiang
Yue
Y
D001565:Benzoates / Q000302:isolation & purification*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides