title: Photochemical keratodesmos for bonding corneal incisions.
abstract: OBJECTIVE
  To evaluate the immediate and long-term effectiveness of a dye-plus-laser irradiation treatment (photochemical keratodesmos [PKD]) for sealing corneal incisions.
  
  
  METHODS
  Incisions (3.5 mm) in rabbit corneas were treated on the incision walls with rose bengal dye followed by exposure to 514-nm laser radiation. PKD was evaluated in three groups (n = 3-6) using laser fluences of 115, 153, or 192 J/cm(2) (180-, 240-, and 300- second exposures, respectively) compared with an untreated group (n = 8). The intraocular pressure at which leakage occurred (IOP(L)) during infusion of saline into the anterior chamber was determined. In a long-term study, treated and control corneas were observed weekly for 10 weeks for the appearance of neovascularization, anterior chamber inflammation, iridocorneal adhesion, corneal melting, and scarring.
  
  
  RESULTS
  Immediately after treatment, the IOP(L) increased with increasing laser fluence, producing IOPs of 230 +/- 90, 370 +/- 120, and more than 500 mm Hg at 115, 153, and 192 J/cm(2), respectively, compared with 40 +/- 20 mm Hg in control eyes (P < 0.005). No reduction in the IOP(L) was observed up to 14 days after surgery. Corneal melting in PKD-treated or control eyes was not observed in the 10-week healing study. Neovascularization, which peaked at 4 weeks but resolved by 8 weeks, was detected around the incision in both PKD-treated and control eyes.
  
  
  CONCLUSIONS
  Immediate and lasting sealing of corneal incisions was obtained in eyes treated with PKD, using short irradiation times. These results suggest that PKD has potential for improved corneal tissue bonding.
authors.affiliation: Massachusetts Eye and Ear Infirmary, The Schepens Eye Research Institute, and Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA.
authors.forename: Cinthia E
authors.initials: CE
authors.lastname: Proaño
authors.forename: Louise
authors.initials: L
authors.lastname: Mulroy
authors.forename: Erika
authors.initials: E
authors.lastname: Jones
authors.forename: Dimitri T
authors.initials: DT
authors.lastname: Azar
authors.forename: Robert W
authors.initials: RW
authors.lastname: Redmond
authors.forename: Irene E
authors.initials: IE
authors.lastname: Kochevar
mesh_terms: D000818:Animals; D003315:Cornea / Q000601:surgery*; D007429:Intraocular Pressure; D007834:Lasers; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000528:radiation effects / Q000627:therapeutic use*; D011817:Rabbits; D012395:Rose Bengal / Q000528:radiation effects / Q000627:therapeutic use*; D013529:Surgical Wound Dehiscence / Q000517:prevention & control*; D014014:Tissue Adhesives; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D017319:Photosensitizing Agents; D014014:Tissue Adhesives; D012395:Rose Bengal