Damage to erythrocytes caused by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (in vitro).
The effects of the exposure of human erythrocytes to different concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin were studied. Particular attention was paid to lipid peroxidation, haemoglobin oxidation, and changes in the activity of catalase and glutathione peroxidase. Human erythrocytes at a 5% haematocrit were incubated with 2,3,7,8-TCDD at concentrations of 0.2 ppm to 1.6 ppm (ng-microg/ml erythrocytes) for 1 hour. The results obtained show that 2,3,7,8-TCDD induces the generation of lipid peroxides and the oxidation of Hb, and decreases the activity of catalase and glutathione peroxidase. This supports the thesis that TCDD causes oxidative stress in erythrocytes.
Bukowska
Bozena
B
Department of the Biophysics of Environmental Pollution, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004785:Environmental Pollutants / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D010084:Oxidation-Reduction; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D013723:Teratogens / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D004785:Environmental Pollutants; D006454:Hemoglobins; D008054:Lipid Peroxides; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase