In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species.
The aim of this study was to determine the effects of indigenous chicken Lactobacillus species isolates from different parts of the gastrointestinal tract on Eimeria tenella invasion in vitro and to characterise the nature of inhibition, if any. The effects of competitive exclusion, steric interference and bacterial extracellular factors on E. tenella invasion were examined in an MDBK cell model. Several Lactobacillus species were initially isolated from chickens and identified by biochemical characteristics and 16S-rRNA. All Lactobacillus species isolates tested, significantly inhibited E. tenella invasion. Steric interference did not affect parasite invasion. Extracellular metabolic factors secreted by Lactobacillus species isolates into the surrounding media were shown to inhibit parasite invasion and these factors appeared to be heat stable. These results show that the natural microflora of poultry can provide a source of E. tenella-inhibiting Lactobacillus species in vitro, and thus may contribute to the control of Eimeria infection.
Tierney
J
J
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University College Dublin, Belfield, 4, Ireland. joanna.tierney@ucd.ie
Gowing
H
H
Van Sinderen
D
D
Flynn
S
S
Stanley
L
L
McHardy
N
N
Hallahan
S
S
Mulcahy
G
G
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000254:growth & development* / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D006358:Hot Temperature; D007779:Lactobacillus acidophilus / Q000502:physiology*; D011201:Poultry Diseases / Q000469:parasitology* / Q000517:prevention & control