Effects of different dietary amounts of LCPUFA n3 and vitamin B6 on lipid composition and antioxidant defences in rat kidney.
Our previous report demonstrated that, when vitamin deficiency is associated with high contents of long chain polyunsaturated fatty acids (LCPUFA) n3, lipid peroxidation susceptibility in rat heart and liver increases. In this paper, we evaluated the effect of the same dietary administration on lipid composition and antioxidant defenses of rat kidney. Results showed that vitamin B(6) deficiency, when associated with a fish oil diet, as compared to vegetable oil condition, increased relative kidney weight and decreased pyridoxal-5P contents. The different LCPUFA n3 dietary contents produced, on kidney phospholipids, effects interlaced with those of vitamin B(6) deficiency; in particular fish oil and vitamin B(6) deficient diet caused a significant decrease of arachidonic acid showing that the processes of elongation and desaturation of linoleic acid were slowed. Also, peroxidation susceptibility was higher, as demonstrated both by increased TBARS formation and glutathione peroxidase activity, and by decreased vitamin E contents and reduced glutathione/oxidized glutathione ratio.
Maranesi
Magda
M
Dipartimento di Biochimica G.Moruzzi, Università degli Studi di Bologna, via Irnerio 48 40126 Bologna, Italy. maranesi@biofarmc.unibo.it
Bochicchio
Davide
D
Zambonin
Laura
L
Tolomelli
Brunella
B
Cabrini
Luciana
L
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007668:Kidney / Q000737:chemistry*; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis*; D008297:Male; D010743:Phospholipids / Q000032:analysis; D010938:Plant Oils / Q000008:administration & dosage; D011732:Pyridoxal Phosphate / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D025101:Vitamin B 6 / Q000008:administration & dosage*; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D008055:Lipids; D010743:Phospholipids; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D011732:Pyridoxal Phosphate; D025101:Vitamin B 6; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase