Promotion of B cell immune responses via an alum-induced myeloid cell population.
Exposure of naïve B cells to the cytokine interleukin-4 (IL-4) and/or antigen leads to a state of "priming," in which subsequent aggregation of major histocompatibility complex class II molecules induces the mobilization of calcium ions and cell proliferation. However, it is not clear how critical this priming is for immune responses or how it is normally induced in vivo. Injection of mice with the commonly used adjuvant alum led to priming of splenic B cells and to the accumulation in the spleen of a previously unknown population of IL-4-producing, Gr1+ cells. These cells and IL-4 were both required for in vivo priming and expansion of antigen-specific B cells, as well as for optimal production of antibody. These studies reveal a key role for a previously unknown accessory myeloid cell population in the generation of humoral immune responses.
Jordan
Michael B
MB
Integrated Department of Immunology, National Jewish Medical and Research Center, University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA.
Mills
David M
DM
Kappler
John
J
Marrack
Philippa
P
Cambier
John C
JC
D000276:Adjuvants, Immunologic; D019264:Adoptive Transfer; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D005620:Freund's Adjuvant; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007114:Immunization; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology*; D009596:Nitrophenols / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000949:Histocompatibility Antigens Class II; D009596:Nitrophenols; D015847:Interleukin-4; D012710:Serum Albumin, Bovine; C041524:aluminum sulfate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005620:Freund's Adjuvant; D002118:Calcium