title: Nasal lavage fluid and proteomics as means to identify the effects of the irritating epoxy chemical dimethylbenzylamine.
abstract: The aims of this study were to describe the changes in the nasal lavage fluid (NLF) protein pattern after exposure to the irritating epoxy chemical dimethylbenzylamine (DMBA) and to identify the affected proteins using a proteomic approach. The protein patterns of NLF from six healthy subjects and eight epoxy workers with airway irritation were analysed using two-dimensional gel electrophoresis (2-DE) before and after exposure to 100 microg m(-3) DMBA for 2 h in an exposure chamber. NLF proteins were identified by (i) comparison with a 2-DE NLF reference database; (ii) N-terminal amino acid sequencing; and (iii) mass spectrometry. In NLF from healthy subjects, the levels of immunoglobulin A increased and the levels of Clara cell protein 16 (CC16) decreased after chamber exposure, while in NLF from epoxy workers, alpha(2)-macroglobulin and caeruloplasmin increased. Two previously unidentified proteins decreased in NLF from epoxy workers after exposure; these were identified as statherin and calgranulin B. In addition, the subjects who developed high counts of eosinophils in their nasal mucosa after chamber exposure had significantly lower levels of immunoglobulin-binding factor (IgBF) before exposure than subjects with low eosinophil infiltration. These results show that short-term exposure to DMBA causes distinct changes in NLF proteins. Moreover, three proteins that have previously not been associated with upper airway irritation were identified: statherin, calgranulin B and IgBF. Further studies are needed to investigate whether these proteins may be used as biomarkers of airway irritation and to give new insight into the ways in which occupational exposure to irritants causes inflammation of the airways.
authors.affiliation: Division of Occupational and Environmental Medicine, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linköping University, Sweden. Mats.Lindahl@imk.liu.se
authors.forename: Mats
authors.initials: M
authors.lastname: Lindahl
authors.forename: Kristina
authors.initials: K
authors.lastname: Irander
authors.forename: Christer
authors.initials: C
authors.lastname: Tagesson
authors.forename: Bengt
authors.initials: B
authors.lastname: Ståhlbom
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000328:Adult; D040502:Calgranulin B / Q000378:metabolism; D002273:Carcinogens; D002570:Ceruloplasmin / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004804:Eosinophils / Q000378:metabolism; D006801:Humans; D007221:Industry; D013058:Mass Spectrometry; D008875:Middle Aged; D017817:Nasal Lavage Fluid; D009297:Nasal Mucosa / Q000473:pathology; D016273:Occupational Exposure; D017434:Protein Structure, Tertiary; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D012907:Smoking; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D040502:Calgranulin B; D002273:Carcinogens; D020543:Proteome; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D000511:alpha-Macroglobulins; D015127:9,10-Dimethyl-1,2-benzanthracene; D002570:Ceruloplasmin