Design and synthesis of a solid-supported FR225659 derivative for its receptor screening.
[structure: see text] We describe the design and synthesis of latex particles attached to an FR225659 derivative to identify its receptor proteins. Two key building blocks were prepared by two-step degradation of FR225659 under basic conditions. The designed ligand showed an acceptable level of biological activity to make it of potential value for use in affinity-supported receptor identification. Affinity purification of FR225659-binding proteins using the latex nanoparticles provided three candidate receptor peptides for the biological activity.
Zenkoh
Tatsuya
T
Department of Applied Chemistry, Tokyo Institute of Technology, Meguro, Tokyo 152-8552, Japan.
Hatori
Hidetaka
H
Tanaka
Hiroshi
H
Hasegawa
Makoto
M
Hatakeyama
Mamoru
M
Kabe
Yasuaki
Y
Setoi
Hiroyuki
H
Kawaguchi
Haruma
H
Handa
Hiroshi
H
Takahashi
Takashi
T
D002352:Carrier Proteins / Q000378:metabolism; D002384:Catalysis; D007202:Indicators and Reagents; D020128:Inhibitory Concentration 50; D007840:Latex / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry
C478488:(4-hydroxy-3-(2-(2-propenyl)-4-quinoline)benzoyl)-(3-chloro-4-hydroxyarginyl)-(3-hydroxy-3-methylprolyl)-dehydrovaline; D002352:Carrier Proteins; D007202:Indicators and Reagents; D007840:Latex; D009842:Oligopeptides