Molecular cloning of a cysteine proteinase cDNA from the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae).
The cotton boll weevil (Anthonomus grandis) causes severe cotton crop losses in North and South America. This report describes the presence of cysteine proteinase activity in the cotton boll weevil. Cysteine proteinase inhibitors from different sources were assayed against total A. grandis proteinases but, unexpectedly, no inhibitor tested was particularly effective. In order to screen for active inhibitors against the boll weevil, a cysteine proteinase cDNA (Agcys1) was isolated from A. grandis larvae using degenerate primers and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed significant homologies with other insect cysteine proteinases. Northern blot analysis indicated that the mRNA encoding the proteinase was transcribed mainly in the gut of larvae. No mRNA was detected in neonatal larvae, pupae, or in the gut of the adult insect, suggesting that Agcys1 is an important cysteine proteinase for larvae digestion. The isolated gene will facilitate the search for highly active inhibitors towards boll weevil larvae that may provide a new opportunity to control this important insect pest.
De Oliveira Neto
Osmundo Brilhante
OB
Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Final W5, Brasília, Brazil.
Batista
João Aguiar Nogueira
JA
Rigden
Daniel John
DJ
Franco
Octávio Luiz
OL
Fragoso
Rodrigo Rocha
RR
Monteiro
Ana Carolina Santos
AC
Monnerat
Rose Gomes
RG
Grossi-De-Sa
Maria Fátima
MF
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D014018:Tissue Distribution; D048889:Weevils / Q000201:enzymology* / Q000235:genetics
D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D012333:RNA, Messenger; D003546:Cysteine Endopeptidases