title: [Screening of antagonist peptide of BLyS from C7C phage display peptide library].
abstract: OBJECTIVE
  To screen an antagonist peptide of BLyS from C7C phage display peptide library.
  
  
  METHODS
  C7C phage display peptide library was screened with BLyS. Indirect ELISA, competitive ELISA and MTT colorimetry were used to identify positive phage clones.
  
  
  RESULTS
  After 3 rounds of screening, the gradual increase of the ratio of output to input and specific enrichment had been achieved. Two phage clones that could inhibit the BLyS activity were identified.
  
  
  CONCLUSIONS
  Two phage display antagonist peptides of BLyS were obtained.
authors.affiliation: Department of Biochemistry & Molecular Biology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ji
authors.forename: Feng-tian
authors.initials: FT
authors.lastname: He
authors.forename: Rong-fen
authors.initials: RF
authors.lastname: Li
authors.forename: Hui-guang
authors.initials: HG
authors.lastname: Gao
authors.forename: Ying-ru
authors.initials: YR
authors.lastname: Zheng
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Zhong
authors.forename: Han-qi
authors.initials: HQ
authors.lastname: Zheng
mesh_terms: D053264:B-Cell Activating Factor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D053264:B-Cell Activating Factor; D008565:Membrane Proteins; D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; C505719:TNFSF13B protein, human; D014409:Tumor Necrosis Factor-alpha