title: Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor.
abstract: Hairy roots of the Chinese herb, Pueraria phaseoloides, obtained from leaf explants and transformed with the Agrobacterium rhizogenes, were cultured in 2.5 l airlift bioreactors for three weeks. Puerarin accumulated at 5,570 microg g(-1) dry wt, which is near 200 times as much as in 250 ml flask cultures. In addition, puearin was exuded into the nutrient medium at final concentrations higher than in the hairy roots themselves.
authors.affiliation: Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. skin@aua.gr
authors.forename: Spiridon
authors.initials: S
authors.lastname: Kintzios
authors.forename: Olga
authors.initials: O
authors.lastname: Makri
authors.forename: Eleni
authors.initials: E
authors.lastname: Pistola
authors.forename: Theodoros
authors.initials: T
authors.lastname: Matakiadis
authors.forename: He Ping
authors.initials: HP
authors.lastname: Shi
authors.forename: Athanassios
authors.initials: A
authors.lastname: Economou
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D007529:Isoflavones / Q000096:biosynthesis*; D018517:Plant Roots / Q000378:metabolism*; D029908:Pueraria / Q000378:metabolism*
chemical_list: D007529:Isoflavones; C033607:puerarin