title: [A case-control study on the polymorphisms of methylenetetrahydrofolate reductase 1298A-->C and susceptibility of esophageal cancer].
abstract: OBJECTIVE
  To investigate the relationship between polymorphisms of methylenetetra-hydrofolate reductase gene 1298A-->C (MTHFR 1298A-->C) and its susceptibility of esophageal cancer (EC).
  
  
  METHODS
  We conducted a case-control study with 141 cases of EC and 228 population-based controls in Huaian city of Jiangsu province, China. Epidemiological data were collected, and DNA of peripheral blood leukocytes was obtained from all of the subjects. MTHFR genotypes were identified by polymerase chain reaction.
  
  
  RESULTS
  (1) The frequency of MTHFR 1298AA, AC and CC genotype were 63.8%, 34.0% and 2.1% in EC and 71.9%, 28.1% and 0.0% in controls, respectively (chi(2)(MH) = 6.69, P = 0.035). The frequency of the MTHFR 1298C allele was 0.19 for EC and 0.14 for controls. (2) Individuals having MTHFR 1298C allele and smoking habit were at a significantly higher risk of developing EC (adjusted OR = 3.48, 95% CI: 1.57 - 7.71) compared with those who having AA genotype but no smoking habit. Individuals having MTHFR 1298C allele and habit of frequent alcohol drinking were at an increased risk of developing EC (adjusted OR = 2.91, 95% CI: 1.20 - 7.08) compared with those with AA genotype and low consumption of alcohol. Individuals having MTHFR 1298C allele but no habit of tea drinking had a 3.52-fold (95% CI: 1.64 - 7.54) increased risk of developing EC compared with tea drinkers with AA genotype. As compared with subjects having AA genotype, low consumption of alcohol, no smoking habit but having habit of drinking tea, the individuals having 1298C allele, habits of frequent alcohol drinking, smoking but no habit of tea drinking had a 12.64-folds (95% CI: 1.39 - 114.65) increased risk of developing EC.
  
  
  CONCLUSIONS
  Results in the present study suggested that there was a coordinated effect between MTHFR 1298 genotypes and habits of smoking, alcohol drinking and tea consumption in the development of EC.
authors.affiliation: Department of Epidemiology, Jiangsu Province Institute of Cancer Research, Nanjing 210009, China.
authors.forename: Chang-ming
authors.initials: CM
authors.lastname: Gao
authors.forename: Takezaki
authors.initials: T
authors.lastname: Toshiro
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Wu
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Cao
authors.forename: Yan-ting
authors.initials: YT
authors.lastname: Liu
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Su-ping
authors.initials: SP
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Su
authors.forename: Xu
authors.initials: X
authors.lastname: Hu
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Kai
authors.forename: Tajima
authors.initials: T
authors.lastname: Kazuo
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking; D016022:Case-Control Studies; D002681:China; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012907:Smoking
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)