title: Alpha v beta 3 integrin expression in human odontoblasts and co-localization with osteoadherin.
abstract: Integrins are heterodimeric transmembrane receptors which promote cell adhesion, thus contributing to the maintenance of tissue organization in both normal and pathological conditions. To characterize the way odontoblasts may interact with other cells and the extracellular matrix in human teeth, we studied expression of alpha v beta 3 integrin, a putative receptor for osteoadherin. We showed that alpha v beta 3 integrin expression was restricted to odontoblasts, blood vessels, and small rounded cells in sound and carious pulp. Odontoblast staining intensity increased from the apical to the cusp region. Osteoadherin staining was strong in the whole odontoblast layer (with a slight decrease in the cusp region) and in predentin. Odontoblasts differentiating in vitro were stained with the anti-alpha v beta 3 integrin antibody, first at the level of intercellular contacts, then throughout the cell membrane. These results suggest that the alpha v beta 3 integrin could play a role in interodontoblast adhesion and odontoblast binding to the surrounding predentin/dentin/pulp matrix, possibly through osteoadherin.
authors.affiliation: Laboratory of Development of Dental Tissues, EA 1892, IFR 62, Faculty of Odontology, Lyon 1 University, G. Paradin Str., 69372 Lyon Cedex 08, France.
authors.forename: M
authors.initials: M
authors.lastname: Lucchini
authors.forename: M-L
authors.initials: ML
authors.lastname: Couble
authors.forename: A
authors.initials: A
authors.lastname: Romeas
authors.forename: M-J
authors.initials: MJ
authors.lastname: Staquet
authors.forename: F
authors.initials: F
authors.lastname: Bleicher
authors.forename: H
authors.initials: H
authors.lastname: Magloire
authors.forename: J-C
authors.initials: JC
authors.lastname: Farges
mesh_terms: D000293:Adolescent; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D039564:Integrin alphaV / Q000235:genetics / Q000378:metabolism*; D039302:Integrin alphaVbeta3 / Q000235:genetics / Q000378:metabolism*; D039661:Integrin beta3 / Q000235:genetics / Q000378:metabolism*; D008964:Molar, Third / Q000166:cytology; D009804:Odontoblasts / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
chemical_list: D016326:Extracellular Matrix Proteins; D039564:Integrin alphaV; D039302:Integrin alphaVbeta3; D039661:Integrin beta3; D021122:Protein Subunits; D011509:Proteoglycans; D012333:RNA, Messenger; C113360:osteoadherin