[Method of detection of soluble HLA-I and soluble HLA-I level alteration in storage blood].
Aim of this study was to develop the detection method of soluble human leukocyte antigens I (sHLA-I) and to explore sHLA-I level alteration in storage blood and its significance. sHLA-I level in sera of 60 Guangdong normal individuals and sHLA-I concentration in blood components from 20 donors quantitatively were detected by sandwich ELISA. The results showed that sensitivity of this assay was 2.84 ng/ml. Coefficients of variation were 5.80% within assays and 9.00% between assays respectively. The recovery rate was >/= 98.57%. The sHLA-I level of normal individuals in Guangdong was (699.54 +/- 360.10) ng/ml. sHLA-I in red blood cells stored for 28 days and in random-donor platelets were significantly higher than that in other blood components and their amount was proportionate to the number of residual donor leukocytes and to the length of storage. In conclusion, sandwich ELISA assay for detection of sHLA-I is a sensitive, specific and stable technique. Blood components with different concentration of sHLA-I may be chosen for clinical transfusion.
Lan
Jiong-Cai
JC
Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Wu
Tao
T
Zhou
Hua-You
HY
Zhang
Yin-Ze
YZ
Wei
Ya-Ming
YM
Lai
Zhi-Fa
ZF
Cao
Qiong
Q
Yang
Quan-Ke
QK
Wu
Da-Lin
DL
Liu
Zhong
Z
D017209:Apoptosis; D001793:Blood Preservation; D004797:Enzyme-Linked Immunosorbent Assay; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology
D015395:Histocompatibility Antigens Class I