title: Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis.
abstract: Sclerotinia sclerotiorum is a ubiquitous necrotrophic fungal pathogen capable of infecting a wide range of plants. To identify genes involved in fungal development and pathogenesis we generated 2232 expressed sequence tags (ESTs) from two cDNA libraries constructed using either mycelia grown in pectin medium or tissues from infected Brassica napus stems. A total of 774 individual fungal genes were identified of which 39 were represented only among the infected plant EST collection. Annotation of 534 unigenes was possible following the categories applied to Saccharomyces cerevisiae and the Universal Gene Ontology scheme. cDNAs were identified that encoded potential pathogenicity factors including four endopolygalacturonases, two exopolygalacturonases, and several metabolite transporters. The potential role of these genes, as well as those encoding signal transduction factors, in the infection process is discussed.
authors.affiliation: Agriculture and Agri-Food Canada,107 Science Place, Saskatoon, Sask., Canada S7N 0X2.
authors.forename: Rugang
authors.initials: R
authors.lastname: Li
authors.forename: Roger
authors.initials: R
authors.lastname: Rimmer
authors.forename: Lone
authors.initials: L
authors.lastname: Buchwaldt
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Sharpe
authors.forename: Ginette
authors.initials: G
authors.lastname: Séguin-Swartz
authors.forename: Cathy
authors.initials: C
authors.lastname: Coutu
authors.forename: Dwayne D
authors.initials: DD
authors.lastname: Hegedus
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D029688:Brassica napus / Q000382:microbiology*; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005656:Fungal Proteins / Q000145:classification / Q000378:metabolism; D015723:Gene Library; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D011096:Polygalacturonase / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D037521:Virulence Factors; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; C020136:exopolygalacturonase