Mechanism of action of the suppression of herpes simplex virus type 2 replication by pterocarnin A.
The purpose of this study was to investigate the in vitro antiviral properties of pterocarnin A, extracted from the bark of Pterocarya stenoptera (Juylandaceae). Results showed that pterocarnin A exhibited anti-herpes simplex virus (HSV) activity. It had a low selectivity index (SI) value and only possessed some level of cell cytotoxic effect at high antiviral concentrations. Mechanism studies demonstrated that pterocarnin A inhibited herpes simplex virus type 2 (HSV-2) from attaching and penetrating into cells. It also actively suppressed HSV-2 multiplication in Vero cells even when added 12 h after infection. This observation indicated that pterocarnin A affected the late stage(s) of HSV-2 infection cycle. Pterocarnin A also significantly reduced viral infectivity at high concentrations. From these observations, it was concluded that pterocarnin A suppressed both early and late in the replication cycle of HSV-2. The various modes of action of pterocarnin A in interfering with certain steps of viral infection thus merit further investigation.
Cheng
Hua-Yew
HY
Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, 807 Kaohsiung, Taiwan, ROC.
Lin
Ta-Chen
TC
Yang
Chien-Min
CM
Wang
Kuo-Chih
KC
Lin
Chun-Ching
CC
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002522:Chlorocebus aethiops; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000633:toxicity; D006027:Glycosides / Q000494:pharmacology* / Q000633:toxicity; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology*; D029525:Juglandaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D014709:Vero Cells; D038301:Virus Inactivation; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D006027:Glycosides; D010936:Plant Extracts; C496413:pterocarnin A; D005707:Gallic Acid