Measurement of large distances in biomolecules using double-quantum filtered refocused electron spin-echoes.
It is shown how the new technique of double-quantum filtered refocused electron spin-echoes is a significant improvement over double-quantum coherence ESR, since it increases the experimental acquisition time. This enables the measurement of longer distances in bilabeled biomolecules. The method is demonstrated on a long double-stranded A-type RNA, spin labeled at both ends. The measured distance of 72 A is in excellent agreement with molecular modeling.
Borbat
Peter P
PP
Department of Chemistry and Chemical Biology, B52 Baker Lab, Cornell University, Ithaca, New York 14853, USA.
Davis
Jared H
JH
Butcher
Samuel E
SE
Freed
Jack H
JH
D020029:Base Pairing; D055592:Biophysical Phenomena; D001703:Biophysics; D018360:Crystallography, X-Ray; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D009569:Nitric Oxide / Q000737:chemistry; D011789:Quantum Theory; D012313:RNA / Q000737:chemistry*; D013891:Thiouridine / Q000737:chemistry
D013891:Thiouridine; D009569:Nitric Oxide; D012313:RNA