Characterization of the replicon of a 51-kb native plasmid from the gram-positive bacterium Leifsonia xyli subsp. cynodontis.
The 4992-bp replicon of a large cryptic plasmid in the gram-positive bacterium Leifsonia xyli subsp. cynodontis was identified and sequenced. The replicon encoded two proteins essential for plasmid replication and stability. The putative replication protein (RepA) is homologous to that of the plasmids in mycobacterial pLR7 family, while the putative ParA protein immediately downstream of RepA is significantly homologous to the Walker-type ATPase required for partition of plasmid and chromosome of the gram-positive bacteria. These two proteins and other ORFs are clustered with the putative promoters and other regulatory sequences, illustrating an efficient organization of the replicon for this novel plasmid.
Li
Tai-Yuan
TY
Department of Biotechnology, College of Life Sciences, Wuhan University, Hubei 430072, PR China.
Yin
Ping
P
Zhou
Yu
Y
Zhang
Yi
Y
Zhang
Yong-Ying
YY
Chen
Tseh-an
TA
D000192:Actinomycetales / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011506:Proteins / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C052883:chromosome partition proteins, bacterial; C045264:replication initiator protein; D004265:DNA Helicases