title: [Application of hard tissue slicing in research of bone tissue engineering].
abstract: OBJECTIVE
  To improve the method of hard tissue slicing for research of bone tissue engineering.
  
  
  METHODS
  The method of hard tissue slicing especially the key technique-slice mounting had been studied and improved.
  
  
  RESULTS
  The experiments showed that our research improved techniques in hard tissue slicing not only in keeping the characteristics of hard tissue slicing, easily stain with HE, Masson, immunohistochemistry, and distinguishing the differences among tissues easily, but also in overcoming the shortcoming of issue shedding easy to happen in the general hard tissue slicing.
  
  
  CONCLUSIONS
  The improved techniques in hard tissue slicing is a more useful hard tissue slicing techniques.
authors.affiliation: Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
authors.forename: Xiuqun
authors.initials: X
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Jingcong
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D005269:Femur / Q000166:cytology; D005456:Fluorescent Dyes; D010006:Osteoblasts; D011817:Rabbits; D011884:Radius / Q000166:cytology; D051381:Rats; D016610:Tissue Embedding; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D005456:Fluorescent Dyes