Bionanotechnology based on silica nanoparticles.
We have developed uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water-in-oil microemulsion method. The nanoparticles are highly luminescent and photostable with the size ranging from 5 nm to 400 nm. Bioconjugation of these silica nanoparticles adds unique biofunctions with various molecules such as enzymes, antibodies, and DNA molecules. Significant advantages have been shown in using bioconjugated nanoparticles for biosensing and bioimaging, such as cell staining, DNA detection and separation, rapid single bacterium detection, and biotechnological application in DNA protection.
Tan
Weihong
W
Center for Research at the Bio/nano Interface, Department of Chemistry, University of Florida, Gainesville 32611, USA. tan@chem.ufl.edu
Wang
Kemin
K
He
Xiaoxiao
X
Zhao
Xiaojun Julia
XJ
Drake
Timothy
T
Wang
Lin
L
Bagwe
Rahul P
RP
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D015415:Biomarkers / Q000737:chemistry; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry; D008280:Magnetics; D008967:Molecular Biology / Q000379:methods*; D036103:Nanotechnology / Q000379:methods* / Q000639:trends; D010316:Particle Size; D038761:Photobleaching; D012822:Silicon Dioxide; D013499:Surface Properties
D015415:Biomarkers; D004396:Coloring Agents; D012822:Silicon Dioxide; D004247:DNA