[Influence of FasL overexpression in transgenic mice on the immune regulative function of Sertoli cell].
OBJECTIVE
To study the influence of FasL overexpression in transgenic mice on the Sertoli cell immune response to testicular infection.


METHODS
Ureaplasma urealyticum (UU) was directly injected into bladders of FasL transgenic mice and wild-type mice respectively, to mimic an ascending infection pathway. At week 1, 2 and 3 after injection, respectively, the mice were put to death to observe the pathological alterations in testis section. And at the same time the differences of FasL, TGF-beta, IL-1alpha and IL-6 expressions on Sertoli cells were compared by immunohistochemical staining between wild mice and transgenic mice before and after infection, respectively. The high-purified Sertoli cells were isolated from the testis tissue of wild-type mice, comparing apoptotic capability of Fas(+) Jurkat cells mediated by FasL(+) Sertoli cells of wild control and wild UU-infected groups.


RESULTS
The pathological changes of testis tissue from transgenic mice were more serious as compared with wild-type mice and the model of cytokines secreted by sertoli cells was distinctive between the two kinds of mice. The UU-infected Sertoli cells increased Fas(+) Jurkat cell apoptosis.


CONCLUSIONS
FasL overexpression can influence the cytokine's secretion in the process of anti-infection immunity and further affects the immune balance of testis locality. Not all FasL over expression is benefit to body's anti-infection immune response.
Liu
Zhao-ping
ZP
Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China.
Li
Wei-yi
WY
Lin
Bo
B
Ma
Ying-hua
YH
Hu
Jia-hua
JH
Chen
Guang-jie
GJ
Xi
Ye-bin
YB
Wang
Bao-guo
BG
Bao
Shi-san
SS
Guo
Li-he
LH
D000818:Animals; D017209:Apoptosis; D016207:Cytokines / Q000378:metabolism*; D053222:Fas Ligand Protein; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D019169:Jurkat Cells / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D012708:Sertoli Cells / Q000378:metabolism*; D013733:Testicular Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D016869:Ureaplasma Infections / Q000378:metabolism / Q000473:pathology*; D016990:Ureaplasma urealyticum
D016207:Cytokines; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007375:Interleukin-1; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D016212:Transforming Growth Factor beta