GATC-specific restriction--modification systems in ruminal bacteria.
The GATC-specific restriction and modification activities were analyzed in 11 major bacterial representatives of ruminal microflora. Modification phenotype was observed in 13 out of 40 ruminal strains. MboI isoschizomeric restriction endonucleases were detected in 10 bacterial strains tested; three strains lacked any detectable corresponding endonuclease activity. The only examined strain of Mitsuokella multi-acida was found to possess a different type of endonuclease activity. This is the first report on restriction activity in ruminal treponemes M. multiacida and Megasphaera elsdenii.
Piknová
M
M
Institute of Chemistry, Faculty of Science, P.J. Safárik University, 040 01 Kosice, Slovakia. piknova@saske.sk
Pristas
P
P
Javorský
P
P
D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D015280:DNA Restriction-Modification Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D045851:Megasphaera / Q000201:enzymology / Q000302:isolation & purification; D012417:Rumen / Q000382:microbiology*; D013379:Substrate Specificity; D045854:Veillonellaceae / Q000201:enzymology / Q000302:isolation & purification
D015280:DNA Restriction-Modification Enzymes; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases