Proliferative and migratory potentials of human cord blood-derived CD34- severe combined immunodeficiency repopulating cells that retain secondary reconstituting capacity.
Using the intra-bone marrow injection (IBMI) technique, we recently identified human cord blood-derived CD34- severe combined immunodeficiency (SCID)-repopulating cells (SRCs) with extensive lymphomyeloid reconstituting ability. In this study, we further investigated the hematopoietic stem cell (HSC) characteristics of these cells in terms of proliferative and migratory potentials. The absolute numbers of CD45+ and CD34+ cells generated by 1 CD34- SRC are significantly higher than those generated by 1 CD34+ SRC. It is interesting that CD34- SRCs have significantly higher migratory and proliferative abilities than CD34+ SRCs. Moreover, only 2 CD34- SRCs transplanted to primary recipients consistently showed secondary reconstituting capacity. This finding suggested the more homogenous nature of CD34- SRCs than that of the population of CD34+ SRCs. These results provided further evidence that CD34- SRCs are functionally different from CD34+ SRCs and that they are a distinct class of primitive HSCs.
Kimura
Takafumi
T
Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Wang
Jianfeng
J
Matsui
Kazuo
K
Imai
Shiro
S
Yokoyama
Shigeki
S
Nishikawa
Mitsuo
M
Ikehara
Susumu
S
Sonoda
Yoshiaki
Y
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001853:Bone Marrow / Q000502:physiology; D002452:Cell Count; D002455:Cell Division; D002465:Cell Movement; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D012038:Regeneration; D016511:Severe Combined Immunodeficiency / Q000473:pathology*; D014183:Transplantation, Heterologous
D018952:Antigens, CD34