Polysaccharide extract isolated from ganoderma lucidum protects rat cerebral cortical neurons from hypoxia/reoxygenation injury.
The effect of polysaccharide extract isolated from Ganoderma lucidum (Gl-PS) on rat cortical neuronal cultures exposed to hypoxia/reoxygenation (H/R) was studied in vitro. Gl-PS (1, 10, 100 microg/ml) increased neuron viability following H/R as measured by the inhibition of MTT reduction. Gl-PS also significantly reduced malondialdehyde content and reactive oxygen species production and increased the manganese superoxide dismutase (Mn-SOD) activity; furthermore, the translocation of nuclear factor-kappa B induced by H/R was blocked. These findings suggest that Gl-PS might be useful in treating H/R-induced oxidative stress and Mn-SOD might play a critical role in the neuroprotective effect of Gl-PS against H/R injury.
Zhao
Hong-Bo
HB
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Lin
Shu-Qian
SQ
Liu
Ji-Hong
JH
Lin
Zhi-Bin
ZB
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D002534:Hypoxia, Brain / Q000473:pathology* / Q000517:prevention & control*; D008315:Malondialdehyde / Q000378:metabolism; D018613:Microscopy, Confocal; D016328:NF-kappa B; D018696:Neuroprotective Agents; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020940:Reishi / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D016328:NF-kappa B; D018696:Neuroprotective Agents; D011134:Polysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C022616:thiazolyl blue; D010100:Oxygen