High resolution DNA flow cytometry of boar sperm cells in identification of boars carrying cytogenetic aberrations.
The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resolution DNA flow cytometry on DAPI-stained sperm cell nuclei (CV </= 1.3%) was investigated as a screening method for the cytogenetic quality of boars. By analyzing a series of 25 animals judged normal by their fertility statistics and a series of seven animals with known reciprocal translocations, a model for identifying sperm cells from cytogenetically aberrant animals was proposed. This model was applied to a series of 50 uncharacterized animals. The model successfully identified a mosaic or chimaeric carrier of an aberrant X chromosome. However, implementation of this technique for screening purposes would necessitate essential improvements in standardization and measurement precision.
Larsen
Jacob
J
Finsen Laboratory, Finsen Centre, Copenhagen University Hospital, Strandboulevarden 49, DK-2100 Copenhagen, Denmark.
Christensen
Knud
K
Larsen
Jørgen K
JK
Jensen
Peter Østrup
PØ
Gustavsson
Ingemar
I
Christensen
Ib Jarle
IJ
D000818:Animals; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D005456:Fluorescent Dyes; D007211:Indoles; D007621:Karyotyping / Q000662:veterinary; D008297:Male; D013094:Spermatozoa / Q000737:chemistry*; D013552:Swine
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA