Reactivation of infectious simian virus 40 from normal human tissues.
In this study, 82 DNA samples of simian virus 40 (SV40)-positive human tumors and normal tissues were transfected into SV40-permissive monkey cells. SV40 wild-type strain 776 was reactivated from two DNA samples, derived from peripheral blood mononuclear cells (PBMCs) of a blood donor and from a vulvar tissue. SV40 reactivation was confirmed by obtaining rescue of SV40 from the DNA of the vulvar tissue in a second transfection experiment. This investigation indicates that infectious SV40 is present in normal human tissues and suggests that (i) PBMCs are probably vectors of SV40 to different tissues of the host and (ii) blood and sexual transmission may be routes of SV40 infection in humans, leading to (iii) virus spread in the human population.
Barbanti-Brodano
Giuseppe
G
Department of Morphology and Embryology, Section of Histology and Embryology, University of Ferrara, Ferrara, Italy.
Martini
Fernanda
F
Corallini
Alfredo
A
Lazzarin
Lorena
L
Trabanelli
Cecilia
C
Vignocchi
Beatrice
B
Calza
Nilla
N
Iaccheri
Laura
L
Morelli
Cristina
C
Tognon
Mauro
M
D000818:Animals; D015342:DNA Probes; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000635:transmission*; D013539:Simian virus 40 / Q000302:isolation & purification*; D014162:Transfection; D014412:Tumor Virus Infections / Q000635:transmission*; D014844:Vulva / Q000821:virology*
D015342:DNA Probes