title: High-level fluoroquinolone resistance in a clinical Streptoccoccus pyogenes isolate in Germany.
abstract: An isolate of Streptococcus pyogenes isolated from a 63-year-old woman with a serious wound infection was found to be highly resistant to fluoroquinolones (levofloxacin MIC > or = 32 mg/L). DNA amplification and sequencing revealed a serine-81 to phenylalanine substitution in gyrA and three substitutions in parC: serine-79 to phenylalanine, aspartic acid-91 to asparagine, and serine-140 to proline. To our knowledge, this is the first report from a European country of a clinical isolate of S. pyogenes with high-level fluoroquinolone resistance.
authors.affiliation: Institute of Medical Microbiology, National Reference Centre for Streptococci, University Hospital, Aachen, Germany. Reinert@rwth-aachen.de
authors.forename: R R
authors.initials: RR
authors.lastname: Reinert
authors.forename: R
authors.initials: R
authors.lastname: Lütticken
authors.forename: A
authors.initials: A
authors.lastname: Al-Lahham
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D005858:Germany; D006801:Humans; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009154:Mutation; D015242:Ofloxacin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D014946:Wound Infection / Q000382:microbiology*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D064704:Levofloxacin; D015242:Ofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase