[Analysis of total proteins in the seed of almond (Prunus dulcis) by two-dimensional electrophoresis].
OBJECTIVE
To analyse the total proteins in the seeds of almond (Prunus dulcis), one of the popular ingestent allergens in China, by two-dimensional electrophoresis.


METHODS
The total proteins of the seeds were extracted by trichloracetic acid (TCA) method, and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension. The spots of proteins were visualized by staining with Coomassie Brilliant Blue R-250.


RESULTS
After analysis with software (ImageMaster 2D), 188 different proteins were detected. The isoelectric points (pI) for approximately 28% of total proteins were between 4.5-5.5, and the relative molecular mass (M(r)) of approximately 62% total proteins were between (20-25)x10(3).


CONCLUSIONS
This was the first high-resolution, two-dimensional protein map of the seed of almond (Prunus dulcis) in China. Our finding has laid a solid foundation for further identification, characterization, gene cloning and standardization of allergenic proteins in the seed of almond (Prunus dulcis).
Li
Dong-dong
DD
Allergy and Inflammation Research Institute, Medical College, Shantou University, Shantou 515031, China.
He
Shao-heng
SH
D000485:Allergens / Q000032:analysis / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007526:Isoelectric Point; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry* / Q000145:classification; D027861:Prunus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D000485:Allergens; D010940:Plant Proteins