A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose.
BACKGROUND
Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma.


METHODS
A liquid-liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C(8) analytical column with UV detection.


RESULTS
A linear response was observed over the concentration range 20-1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components.


CONCLUSIONS
This rapid method (run time<13 min) is currently used for poison management involving hydroxyzine.
Péhourcq
Fabienne
F
Department of Clinical Pharmacology and Toxicology, CHU Pellegrin, Place Amélie Raba-Léon, 33076 Bordeaux Cedex, France. fabienne.pehourcq@chu-bordeaux.fr
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062787:Drug Overdose / Q000097:blood*; D005260:Female; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D006919:Hydroxyzine / Q000097:blood*; D008297:Male
D006634:Histamine H1 Antagonists; D006919:Hydroxyzine