title: Direct measurement of BDP and 17-BMP in bronchial and peripheral lung tissue after inhalation of HFA- vs CFC-driven aerosols.
abstract: Indirect assessments have shown a superior lung deposition of HFA-BDP (Ventolair/Qvar) compared to CFC-BDP (Aerobec). The aim of this study was to assess the concentrations of BDP and its metabolite 17-BMP in airways and peripheral tissue from resected lung specimens after inhalation of these BDP formulations. Immediately prior to surgery for lung cancer, 10 patients inhaled 1000 microg of either CFC-BDP (n = 5) or HFA-BDP (n = 5) Mouthwash was collected after inhalation, and serum before, during, and after surgery. There was no significant difference between CFC and HFA in the concentration of 17-BMP in bronchi (median, 4365 vs 4121 pg/g tissue). After CFC, concentrations of 17-BMP were lower in peripheral tissue (1424 vs 2089 pg/g; ANCOVA, p = 0.001) and in serum taken immediately after inhalation (688 vs 1219 pg/ml, p < 0.01). Furthermore, the CFC group showed a higher concentration of BDP in the mouthwash (17,660 vs 1320 ng/ml, p < 0.05), but the concentration of 17-BMP was lower (452 vs 1028 ng/ml, n.s.). These findings indicate a predominantly peripheral deposition of HFA-BDP, in line with previous data. They also provide evidence for a faster uptake and metabolism of HFA-BDP, probably because BDP is dissolved in HFA and has a smaller particle size distribution than the CFC suspensions.
authors.affiliation: Research Laboratory, Center for Pneumology and Thoracic Surgery, Hospital Grosshansdorf, Wöhrendamm 80, D-22927 Grosshansdorf, Germany. o.holz@puloresearch.de
authors.forename: O
authors.initials: O
authors.lastname: Holz
authors.forename: I
authors.initials: I
authors.lastname: Zühlke
authors.forename: M
authors.initials: M
authors.lastname: Einhaus
authors.forename: L
authors.initials: L
authors.lastname: Welker
authors.forename: F
authors.initials: F
authors.lastname: Kanniess
authors.forename: D
authors.initials: D
authors.lastname: Branscheid
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
authors.forename: L I
authors.initials: LI
authors.lastname: Harrison
authors.forename: R A
authors.initials: RA
authors.lastname: Jörres
authors.forename: K
authors.initials: K
authors.lastname: Richter
authors.forename: H
authors.initials: H
authors.lastname: Magnussen
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000335:Aerosol Propellants / Q000737:chemistry*; D000368:Aged; D001507:Beclomethasone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics*; D001980:Bronchi / Q000737:chemistry; D017402:Chlorofluorocarbons / Q000737:chemistry*; D004337:Drug Carriers; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D008168:Lung / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D013997:Time Factors; D014018:Tissue Distribution; D005466:Fluorocarbons
chemical_list: D000335:Aerosol Propellants; D001507:Beclomethasone; D017402:Chlorofluorocarbons; D004337:Drug Carriers; D005938:Glucocorticoids; D006845:Hydrocarbons, Fluorinated; C065857:beclomethasone 17-monopropionate; C094049:apaflurane; D005466:Fluorocarbons