title: Plasma membrane stress failure in ventilator-injured lungs. A hypothesis about osmoregulation and the pharmacologic protection of the lungs against deformation injury.
abstract: Cell injury and repair are invariable consequences of mechanical ventilation with large tidal volumes. Rate and amplitude of deforming stress affect numerous cell metabolic functions including host defense and wound repair. Recently, we have focused on the role of plasma membrane stress failure as a trigger for a pro-inflammatory response in mechanically ventilated lungs. We have developed both cell- and organ-based models to study this problem. Alveolar epithelial cells that are exposed to deforming stresses seek to maintain sublytic plasma membrane tension and may activate mechanisms of cell surface area regulation to control membrane tension. Interventions which either increase the amount of excess plasma membrane or enhance lipid trafficking should be cytoprotective against deformation induced injury. Osmotic manipulation may be one such intervention. Preconditioning the lungs with anisosmotic solutions may allow the cells to recruit excess plasma membrane and thus be more resistant to ventilator-induced lung injury.
authors.affiliation: Department of Medicine, Division of Pulmonary and Critical Care Medicine, Thoracic Diseases Research Unit, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Mendez
authors.forename: Otis B
authors.initials: OB
authors.lastname: Rickman
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Hubmayr
mesh_terms: D002462:Cell Membrane / Q000502:physiology*; D004847:Epithelial Cells / Q000648:ultrastructure; D006801:Humans; D008171:Lung Diseases / Q000209:etiology* / Q000473:pathology; D009994:Osmolar Concentration; D011650:Pulmonary Alveoli / Q000648:ultrastructure; D012121:Respiration, Artificial / Q000009:adverse effects*; D014882:Water-Electrolyte Balance