Gluten of spelt wheat (Triticum aestivum subspecies spelta) as a source of peptides promoting viability and product yield of mouse hybridoma cell cultures.
The enzymic hydrolysate of gluten from spelt wheat (Triticum aestivum subsp. spelta), an ancient protein-rich wheat subspecies, was subjected to repeated chromatography runs on the small pore size exclusion chromatography matrix, Biogel P-2. Two small peptide fractions were purified by rechromatography. The amino acid analyses carried out upon total hydrolysis of these fractions have shown a very high proportion of glutamic acid/glutamine, leucine, and methionine. The biological activity of the peptide fractions was tested on a model hybridoma at a concentration range from 0.02 to 0.2%. The most striking effect of peptide fractions, apparent even at the lowest concentrations tested, was a significantly higher persistence of viable cells on day 6, i.e., at the decline phase of the cultures. Culture viability values in the presence of peptide fractions were 64-74%, in comparison with 56% in the control culture. The results of this work are consistent with the concept that peptide molecules may act as antiapoptotic agents, survival factors, rather than serving as metabolic substrates.
Franek
Frantisek
F
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Radiova 1, CZ-10227 Prague 10, Czech Republic. franek@biomed.cas.cz
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018698:Glutamic Acid / Q000032:analysis; D005973:Glutamine / Q000032:analysis; D005983:Glutens / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology* / Q000187:drug effects*; D006868:Hydrolysis; D007930:Leucine / Q000032:analysis; D008715:Methionine / Q000032:analysis; D051379:Mice; D010446:Peptide Fragments / Q000032:analysis / Q000494:pharmacology*; D014908:Triticum / Q000737:chemistry*
D000596:Amino Acids; D010446:Peptide Fragments; D005973:Glutamine; D018698:Glutamic Acid; D005983:Glutens; D008715:Methionine; D007930:Leucine