title: Synthesis of oligodeoxynucleotides containing a single diastereoisomer of alpha-(N2-2'-deoxyguanosinyl)-N-desmethyltamoxifen.
abstract: A phosphoramidite chemical synthesis of oligodeoxynucleotides containing a diastereoisomer of (E)-alpha-(N(2)-deoxyguanosinyl)-N-desmethyltamoxifen, a major tamoxifen (TAM)-derived DNA adduct in animal and women treated with TAM, was described. The site-specifically modified oligodeoxynucleotide can be used for mutagenesis, DNA repair, and 3D structural studies and also as standard for quantitative analysis of TAM-DNA adducts in animal and human.
authors.affiliation: Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-8651, USA.
authors.forename: Y R Santosh
authors.initials: YR
authors.lastname: Laxmi
authors.forename: Naomi
authors.initials: N
authors.lastname: Suzuki
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Shinya
authors.initials: S
authors.lastname: Shibutani
mesh_terms: D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts; D003849:Deoxyguanosine; D006801:Humans; D008968:Molecular Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology*; D013237:Stereoisomerism; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000494:pharmacology
chemical_list: D018736:DNA Adducts; D009838:Oligodeoxyribonucleotides; D013629:Tamoxifen; D003849:Deoxyguanosine