Photoreactivation of Legionella pneumophila after inactivation by low- or medium-pressure ultraviolet lamp.
Photoreactivation of Legionella pneumophila after the inactivation by low-pressure (LP) or medium-pressure (MP) UV lamp was investigated in comparison with that of Escherichia coli. An endonuclease sensitive site (ESS) assay was used to determine the number of UV-induced pyrimidine dimers in the genome DNA of L. pneumophila or E. coli, while the survival ratio of each bacterium was also investigated by cultivation methods. L. pneumophila performed photoreactivation with almost complete repair of pyrimidine dimers associated with the quick recovery of survival ratio. A 3 log inactivation of L. pneumophila by LP or MP UV lamp was, respectively, resulted in 0.5 log or 0.4 log inactivation when photoreactivation was completed. Interestingly, L. pneumophila performed equivalent photoreactivation after LP and MP UV lamp exposures while photoreactivation of E. coli was significantly repressed after the inactivation by MP UV lamp. This study indicated that an attention would be required to design and operate a UV disinfection system targeting L. pneumophila. It was further implied that E. coli would not correctly indicate the fate of L. pneumophila in UV disinfection systems when photoreactivation takes place.
Oguma
Kumiko
K
Department of Urban Engineering, Graduate school of Engineering, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 113-8656, Japan. oguma@env.t.u-tokyo.ac.jp
Katayama
Hiroyuki
H
Ohgaki
Shinichiro
S
D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000472:pathogenicity; D016952:Legionella pneumophila / Q000472:pathogenicity*; D010777:Photochemistry; D011312:Pressure; D016019:Survival Analysis; D014466:Ultraviolet Rays; D018508:Water Purification / Q000379:methods*
D004269:DNA, Bacterial