title: Molecular identification of Cryptosporidium spp. in animal and human hosts from the Czech Republic.
abstract: To study the diversity of Cryptosporidium spp. in various hosts, we used the variability of the small-subunit rRNA gene and the Cryptosporidium oocyst wall protein genes. Oocysts from humans, cattle, horses, dogs, field mice, chickens, reptiles, deer, goat, cat, antelope and from a sample of water reservoir were assayed. The zoonotic C. parvum bovine genotype sequence was found to be present in the most of isolates. This study shows a complex epidemiology pattern for C. parvum bovine genotype infections. The identification of cattle, horse, and deer isolates emphasizes a transmission route for C. parvum via these hosts, and identifies a potential source for human infection in the Czech Republic. Furthermore, C. andersoni from a cow, C. baileyi from a chicken, C. felis from a cat, C. meleagridis from a dog, and C. saurophilum and C. serpentis from reptiles were also identified in the isolates from the Czech Republic.
authors.affiliation: Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Hajdusek
authors.forename: Oleg
authors.initials: O
authors.lastname: Ditrich
authors.forename: Jan
authors.initials: J
authors.lastname: Slapeta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D018153:Czech Republic / Q000453:epidemiology; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012335:RNA, Ribosomal; C106305:oocyst wall protein, Cryptosporidium