title: IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.
abstract: In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.
authors.affiliation: Leprosy Research Center, National institute of Infectious Diseases, Tokyo, Japan. fukutomi@nih.go.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Fukutomi
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Fumishige
authors.initials: F
authors.lastname: Minagawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Toratani
authors.forename: Gregory
authors.initials: G
authors.lastname: McCormick
authors.forename: James
authors.initials: J
authors.lastname: Krahenbuhl
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D003470:Culture Media; D016753:Interleukin-10 / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000382:microbiology*; D051379:Mice; D009166:Mycobacterium leprae / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature
chemical_list: D003470:Culture Media; D016753:Interleukin-10