Comparison of the expression of Langerin and 175 kD mannose receptor in antigen-presenting cells in normal human skin and basal cell carcinoma.
The presence of professional antigen-presenting cells in tumours can influence their further spreading. Location of cells exhibiting a specific marker of Langerhans cells--Langerin, and the 175 kD mannose receptor as a marker of dendritic cells of non-Langerhans type and macrophages, was studied using double staining in the normal human epidermis and in basal cell carcinomas. The Langerin-positive cells strictly colonized the epidermis and no cells were found in the dermis, where 175 kD mannose receptor-exhibiting cells were present. Very rare elements in the epidermal/dermal interface were positive for both markers. A low incidence of Langerin-positive cells was found in tumours and 1/3 of studied carcinomas were even Langerhans cell-free. The extraepithelial presence of Langerin-positive cells forming contacts with dendrite-like protrusions of 175 kD mannose receptor-exhibiting cells was found in connective tissue surrounding the tumour epithelium and indicates possible cooperation of both elements.
Plzáková
Z
Z
Charles University, 1st Faculty of Medicine, Institute of Anatomy, Prague, Czech Republic.
Chovanec
M
M
Smetana
K
K
Plzák
J
J
Stork
J
J
Saeland
S
S
D000938:Antigen-Presenting Cells / Q000378:metabolism*; D015703:Antigens, CD; D000954:Antigens, Surface / Q000378:metabolism*; D002280:Basal Cell Carcinoma / Q000378:metabolism*; D020405:Dermis / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*
D015703:Antigens, CD; D000954:Antigens, Surface; C121916:CD207 protein, human; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface