Investigation of killer cell immunoglobulin-like receptor gene diversity: II. KIR2DS4.
A polymerase chain reaction sequence-specific oligonucleotide probe typing method identifying and distinguishing alleles of the KIR2DS4 gene has been established. The system is based on the specific amplification of a region of this gene, followed by hybridization with 11 sequence-specific oligonucleotide probes. The method has been applied to a healthy group of Northern Irish caucasian individuals, establishing frequencies of alleles of this locus within the local population. Furthermore, cell line DNA and families from the 13th International Histocompatibility Workshop, in addition to local families, have also been allele typed at the KIR2DS4 locus. Haplotype segregation, with respect to KIR2DS4 alleles, has been examined by using the local family data. Within all sample groups investigated, four KIR2DS4 alleles were identified, two of which are novel to this investigation.
Maxwell
Lynn D
LD
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland.
Williams
Fionnuala
F
Gilmore
Paula
P
Meenagh
Ashley
A
Middleton
Derek
D
D000483:Alleles; D005787:Gene Frequency / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009660:Northern Ireland; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci / Q000235:genetics* / Q000276:immunology; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology; D054340:Receptors, KIR; D044465:White People
C473953:KIR2DS4 protein, human; D015345:Oligonucleotide Probes; D011971:Receptors, Immunologic; D054340:Receptors, KIR