[Comparison of serological procedures used for the diagnosis of viral exanthema in laboratories participating in the measles elimination plan].
OBJECTIVE
The comparison of serological methods used by the laboratories participating in the Network for the Elimination of Measles to diagnose measles virus infection as well as differential diagnosis with other exanthematic diseases are compared.


METHODS
One panel of 20 serum samples including measles (12), rubella (4), parvovirus B19 (2) and dengue (2) infections was established. All cases were diagnosed by detection of specific IgM. The panel was sent to the laboratories of the Network. The results were compared with those obtained at the reference laboratory.


CONCLUSIONS
Regarding measles, IgM response from 20 laboratories (19 by ELISA and 1 by indirect immunofluorescence) was obtained, with an agreement of 91.5%. Related to rubella IgM, replay from 6 laboratories, using ELISA, was received, with an agreement of 98.7%. With respect to parvovirus B19 IgM, response from 10 laboratories (8 by ELISA and 2 by indirect immunofluorescence) was obtained, with an agreement of 94.6%. Results about dengue virus were not reported by any laboratory.


CONCLUSIONS
Some laboratories from the network should review the methods used for the diagnosis of measles and other exanthematic diseases. The results reassert the need for a reference laboratory to support confirmation of the results.
de Ory
Fernando
F
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain. fory@isciii.es
Sanz
Juan Carlos
JC
Echevarría
Juan Emilio
JE
Mosquera
María del Mar
Mdel M
Guisasola
María Eulalia
ME
D000914:Antibodies, Viral / Q000097:blood*; D003715:Dengue / Q000097:blood / Q000175:diagnosis; D003716:Dengue Virus / Q000276:immunology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005076:Exanthema / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D005077:Exanthema Subitum / Q000097:blood / Q000175:diagnosis; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D017053:Infection Control / Q000458:organization & administration; D007753:Laboratories / Q000706:statistics & numerical data; D008457:Measles / Q000097:blood / Q000175:diagnosis* / Q000517:prevention & control; D008459:Measles virus / Q000276:immunology; D016732:Parvovirus B19, Human / Q000276:immunology; D011237:Predictive Value of Tests; D012409:Rubella / Q000097:blood / Q000175:diagnosis; D012412:Rubella virus / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D013030:Spain
D000914:Antibodies, Viral; D007075:Immunoglobulin M