Molecular discrimination of medicinal Astragali radix by RAPD analysis.
The randomly amplified polymorphic DNA (RAPD) analysis has been applied for estimating genetic diversity in plant populations or cultivars. To discriminate geographical origin among Astragali radix populations, RAPD analysis was carried out using 20 mer-random primers. The similarity coefficient between the DNA of Astragali radix plants analyzed was 0.527. Although the coefficients of similarity were high, primer 7, 8 and 10 gave distinguishable bands between Korean and Chinese Astragali radix. We obtained the specific RAPD markers to discriminate between Korean and Chinese Astragali radix at a DNA level. These results suggest that this method is able to discriminate the concerned Astragali radix geographical origin species. Also, this is the first report on the genetic diversity in geographical origin among Astragali radix populations using RAPD analysis. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.
Na
Ho-Jeong
HJ
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.
Um
Jae-Young
JY
Kim
Sung-Chul
SC
Koh
Kang-Hoon
KH
Hwang
Woo-Jun
WJ
Lee
Kang-Min
KM
Kim
Cheorl-Ho
CH
Kim
Hyung-Min
HM
D027885:Astragalus propinquus / Q000235:genetics*; D001483:Base Sequence; D002681:China; D018744:DNA, Plant / Q000235:genetics; D005828:Genetics, Population; D007723:Korea; D010946:Plants, Medicinal / Q000235:genetics; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant