title: In vitro study of the antioxidant and immunomodulatory activity of aqueous infusion of Bidens pilosa.
abstract: Bidens pilosa is an annual plant from tropical America with anti-inflammatory properties in hepatitis, laryngitis, headache and digestive disorders, among others. Its wide pharmacological applications can be attributed to its chemical composition, with inhibitory effects on pathogenic microorganisms and flavonoids, which show strong antioxidant capacities. We investigated the antioxidant activity of an aqueous infusion of Bidens pilosa by studying its protective effect on the hemolysis induced by an initiator of radicals such as 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The immunomodulatory activity of the infusion was tested using whole blood cells. Cytokine production increased in whole blood stimulated or not by lipopolysaccharides (LPSs). The infusion is also characterized by its capacity to protect erythrocytes from the phototoxic effect of chlorpromazine, which allows its use as a potential photoprotector. Finally, it did not show ocular irritation, as demonstrated by the effect on hemoglobin denaturation. This study supports the health benefits of the ingestion of the infusion.
authors.affiliation: Department of Fisiologia-Divisió, IV, Facultat de Farmàcia, Av. Joan XXIII s/n, E-08028 Barcelona, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Abajo
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Boffill
authors.forename: Jaime
authors.initials: J
authors.lastname: del Campo
authors.forename: María
authors.initials: M
authors.lastname: Alexandra Méndez
authors.forename: Yisel
authors.initials: Y
authors.lastname: González
authors.forename: Montserrat
authors.initials: M
authors.lastname: Mitjans
authors.forename: María
authors.initials: M
authors.lastname: Pilar Vinardell
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000578:Amidines; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D031176:Bidens; D002746:Chlorpromazine; D006461:Hemolysis; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000276:Adjuvants, Immunologic; D000578:Amidines; D000975:Antioxidants; D010936:Plant Extracts; C046728:2,2'-azobis(2-amidinopropane); D002746:Chlorpromazine