Micturition-related neuronal firing in the periaqueductal gray area in cats.
The midbrain periaqueductal gray (PAG) is the area promoting emotional motor responses, reproductive behaviors and analgesia. Recent studies suggest that neurons in the PAG may be crucial for regulating the micturition reflex in both experimental animals and humans. We examined single neuronal activities in the PAG and the adjacent area in response to isovolumetric spontaneous micturition reflexes in 20 supracollicular decerebrated cats. In total, 84 neurons were recorded in the PAG that were related to urinary storage/micturition cycles. Of the neurons recorded, the most common were tonic storage neurons (43%), followed by tonic micturition neurons (29%), phasic storage neurons (15%) and phasic micturition neurons (13%). In addition to the tonic/phasic as well as storage/micturition classification, the neurons showed diverse discharge patterns: augmenting, constant and decrementing, with the constant discharge pattern being most common. Of the 16 neurons located within the PAG that had similar discharge patterns to those just ventral to the PAG, the micturition neurons were distributed in a broader area, whereas the storage neurons seemed to be concentrated in the middle part of the PAG (P0-1, Horsley-Clarke coordinate). High-frequency stimulation (HFS; 0.2-ms duration, 100 Hz) applied in the PAG elicited inhibition of the micturition reflex. Effective amplitude of the electrical stimulation for evoking inhibitory responses was less than 50 microA. In conclusion, the results of the present study showed that HFS of the PAG inhibited the micturition reflex and there were micturition-related neuronal firings in the PAG in cats, suggesting that the PAG is involved in neural control of micturition.
Liu
Z
Z
Department of Neurology, Chiba University, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
Sakakibara
R
R
Nakazawa
K
K
Uchiyama
T
T
Yamamoto
T
T
Ito
T
T
Hattori
T
T
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001931:Brain Mapping; D002415:Cats; D004558:Electric Stimulation / Q000379:methods; D004576:Electromyography / Q000379:methods; D008297:Male; D009130:Muscle, Smooth / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000145:classification / Q000502:physiology* / Q000528:radiation effects; D010487:Periaqueductal Gray / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D011149:Pons / Q000502:physiology / Q000528:radiation effects; D012018:Reflex / Q000502:physiology; D001743:Urinary Bladder / Q000502:physiology*; D014554:Urination / Q000502:physiology*