Rapid serological profiling by an immunocomb-based dot-enzyme-linked immunosorbent test for three major poultry diseases.
An immunocomb-based dot-ELISA, employing specially designed apparatus, was used to measure the antibody status for the three major poultry diseases--Newcastle disease, infectious bursal disease and infectious bronchitis--in single test sera. Positive samples could be classified into strong, moderate and weak positives by comparison with the colour reaction given by known strong and weak positive serum controls. The simultaneous dot-immunobinding assay gave reproducible results and allowed considerable savings on the cost of reagents compared to liquid ELISA. The antigen-coated immunocomb can be stored under refrigeration and the test can be performed rapidly under field conditions by trained personnel.
Manoharan
S
S
Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tanuvas Madhavaram Milk Colony, Chennai 600 051, India. ulagaimano@yahoo.com
Parthiban
M
M
Prabhakar
T G
TG
Ravikumar
G
G
Koteeswaran
A
A
Chandran
N D J
ND
Rajavelu
G
G
D000818:Animals; D000956:Antigens, Viral; D018175:Birnaviridae Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D018352:Coronavirus Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D001351:Infectious bronchitis virus / Q000302:isolation & purification; D007243:Infectious bursal disease virus / Q000302:isolation & purification; D009521:Newcastle Disease / Q000097:blood* / Q000821:virology; D009522:Newcastle disease virus / Q000302:isolation & purification; D011201:Poultry Diseases / Q000097:blood / Q000821:virology*
D000956:Antigens, Viral