title: Unilateral coronal synostosis: a histomorphometric study.
abstract: OBJECTIVE
  This histomorphometric study compared the open and prematurely fused side of the coronal suture in subjects with unilateral coronal synostosis (UCS).
  
  
  METHODS
  Sutures and parasutural bone were obtained from seven subjects with nonsyndromic UCS during operative correction at 3 to 24 months of age. Histological and cellular analyses were performed for the affected and open sutures. Specimens were examined by light and polarizing microscopy. Sutural patterns, osseous morphology, calvarial thickness, tartrate-resistant acid phosphatase (TRAP)-positive cells, and marrow spaces were evaluated histomorphologically, qualitatively, and semiquantitatively. Histomorphometry was performed to determine total projected area of marrow space as a percentage of unit area, total number of TRAP-positive cells per specimen, and perisutural cranial thickness.
  
  
  RESULTS
  Polarizing microscopy showed that affected sutures were composed of more lamellar bone than the normal sutures. By light microscopy, the clinically fused sutures were 1.7-fold thicker (p <.02), had twofold larger marrow spaces (p <.0006), and contained sixfold more TRAP-positive osteoclasts in marrow spaces near the suture (p <.04) than the normal sutures. Quantitative analysis of the normal sutures revealed that calvarial thickness was greater with age and that there was an inverse correlation between medullary area and age. For the affected sutures, there was also an age-related increase in calvarial thickness. There were also trends for age-related declines in numbers of osteoclasts in both open and affected sides.
  
  
  CONCLUSIONS
  These results question the hypothesis that defective osteoclastic activity is pivotal in the pathogenesis of UCS and support the hypothesis that this condition results from abnormally active bony remodeling.
authors.affiliation: Department of Growth and Development, Harvard School of Dental Medicine, Boston, Massachusetts, USA.
authors.forename: H
authors.initials: H
authors.lastname: Vastardis
authors.forename: J B
authors.initials: JB
authors.lastname: Mulliken
authors.forename: J
authors.initials: J
authors.lastname: Glowacki
mesh_terms: D015519:Bone Density; D016723:Bone Remodeling; D002675:Child, Preschool; D003393:Cranial Sutures / Q000473:pathology*; D003398:Craniosynostoses / Q000473:pathology* / Q000601:surgery; D003399:Craniotomy; D005260:Female; D006801:Humans; D007223:Infant; D016014:Linear Models; D008297:Male; D010010:Osteoclasts / Q000473:pathology / Q000502:physiology