Intrinsic factor-mediated modulation of cyanocobalamin-N-sulfonyl-acridinium-9-carboxamide chemiluminescence.
Two cyanocobalamin-N-sulfonyl-acridinium-9-carboxamides with linkage through the N(10) or 9-position were prepared from B(12)-e-carboxylic acid. The noncovalent association of intrinsic factor with these ligands resulted in specific modulation of the associated chemiluminescence signal either by quenching or changing the emission profile. Either effect was sufficient to formulate a homogeneous assay to detect vitamin B(12) in buffer.
Adamczyk
Maciej
M
Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Johnson
Donald D
DD
Mattingly
Phillip G
PG
Moore
Jeffrey A
JA
Pan
You
Y
D000166:Acridines / Q000737:chemistry / Q000494:pharmacology; D007437:Intrinsic Factor / Q000187:drug effects*; D049449:Luminescence; D008958:Models, Molecular; D008968:Molecular Conformation; D013329:Structure-Activity Relationship; D014805:Vitamin B 12 / Q000737:chemistry / Q000494:pharmacology
D000166:Acridines; D007437:Intrinsic Factor; D014805:Vitamin B 12