Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.
Terakawa
Mitsuhiro
M
Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. tera@obara.elec.keio.ac.jp
Ogura
Makoto
M
Sato
Shunichi
S
Wakisaka
Hitoshi
H
Ashida
Hiroshi
H
Uenoyama
Maki
M
Masaki
Yoshinori
Y
Obara
Minoru
M
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects*; D002463:Cell Membrane Permeability / Q000528:radiation effects*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins; D007834:Lasers; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics*; D013314:Stress, Mechanical; D013696:Temperature; D014162:Transfection / Q000379:methods*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins