title: The effect of inoculum age and solid versus liquid propagation on inoculum quality of an industrial Bacillus licheniformis strain.
abstract: Shorter lag phases were obtained in cultivations of Bacillus licheniformis using early-compared to late-stationary growth phase inocula and using liquid versus solid propagation medium. Flow cytometry and fluorescence ratio imaging microscopy (FRIM) after staining with 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), confirmed that liquid early-stationary growth phase inoculum had a higher vitality and was more homogeneous than solid late-stationary growth phase inoculum. DNA-microarray analyses indicated that liquid early-stationary growth phase inoculum was in a more active state in terms of cell multiplication whereas solid late-stationary growth phase inoculum was induced to some spore formation potentially causing delayed growth initiation.
authors.affiliation: Novozymes A/S, Bagsvaerd, Denmark. tho@kvl.dk
authors.forename: Tina
authors.initials: T
authors.lastname: Hornbaek
authors.forename: Allan K
authors.initials: AK
authors.lastname: Nielsen
authors.forename: Jens
authors.initials: J
authors.lastname: Dynesen
authors.forename: Mogens
authors.initials: M
authors.lastname: Jakobsen
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000254:growth & development*; D004798:Enzymes / Q000096:biosynthesis; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D007218:Industrial Microbiology; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic
chemical_list: D004798:Enzymes