Evaluation of antifungal efficacy in an optimized animal model of Trichophyton mentagrophytes-dermatophytosis.
Dermatophytoses are known to cause considerable discomfort, cosmetic problems and financial loss that have been recognized as a significant health concern worldwide. Since currently available antifungal agents have limitations in their efficacy, new agents are being developed. This study was undertaken to optimize an in vivo model of experimental dermatophytosis for evaluation of the efficacy of antifungal compounds. Guinea pigs were infected with different inocula of T. mentagrophytes to establish dermatophytosis. The optimal conditions for dermatophytosis in guinea pigs were found to be an inoculum size of 1 x 10(7) fungal cells applied on abraded skin. After optimization, animals were treated with oral or topical formulations of terbinafine. The optimized guinea pig model was found to be highly reproducible, and useful in the primary screening and evaluation of the anti-dermatophytic efficacy of topical and oral formulations of antifungal agents.
Ghannoum
M A
MA
Center for Medical Mycology, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106-5028, USA. mag3@cwru.edu
Hossain
M A
MA
Long
L
L
Mohamed
S
S
Reyes
G
G
Mukherjee
P K
PK
D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006168:Guinea Pigs; D008297:Male; D008826:Microbial Sensitivity Tests; D009281:Naphthalenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000077291:Terbinafine; D014005:Tinea / Q000188:drug therapy*; D014249:Trichophyton / Q000187:drug effects*
D000935:Antifungal Agents; D009281:Naphthalenes; D000077291:Terbinafine