An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.
MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.
Liu
Chang-Gong
CG
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Calin
George Adrian
GA
Meloon
Brian
B
Gamliel
Nir
N
Sevignani
Cinzia
C
Ferracin
Manuela
M
Dumitru
Calin Dan
CD
Shimizu
Masayoshi
M
Zupo
Simona
S
Dono
Mariella
M
Alder
Hansjuerg
H
Bullrich
Florencia
F
Negrini
Massimo
M
Croce
Carlo M
CM
D000328:Adult; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005333:Fetus / Q000378:metabolism; D020869:Gene Expression Profiling; D016678:Genome; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009369:Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D009928:Organ Specificity; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D035683:MicroRNAs; D015345:Oligonucleotide Probes