HPLC of tryptophan and its metabolites: as OPA derivatives and on the basis of their UV and fluorescence spectra, simultaneously.
A HPLC method was developed for the quantitation of tryptophan and its metabolites, in total sixteen compounds, belonging both to the indolyl and kynurenine pathways. Primary amino group containing metabolites have been quantitated as their o-phthaldialdehyde/3-mercaptopropionic acid derivatives (5-hydroxytryptophan, tryptophan, 5-hydroxytryptamine, tryptamine), others without derivatization (quinolinic-, nicotinic-, antranilic-, xanthurenic-, kynurenic, indoleacetic- and indolepropionic acids, nicotineamide, melatonin, tryptophol, indole, methyl-indole), in a single run, within 20 min. Fluorescence and UV detections were performed simultaneously.
Presits
Peter
P
Institute of Inorganic & Analytical Chemistry, L. Eötvös University, H-1518, Budapest 112, PO Box 32, Hungary.
Molnar-Perl
Ibolya
I
D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism*; D009764:o-Phthalaldehyde / Q000032:analysis* / Q000378:metabolism*
D009764:o-Phthalaldehyde; D014364:Tryptophan