Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1.
We performed high-throughput in situ hybridization screening of sections of mouse epidermis using an equalized skin cDNA library as probes and identified a novel gene giving rise to two splicing variants, both of which are expressed in the spinous layer. This gene was mapped between two genes encoding keratinocyte-related peptides, suprabasin and keratinocyte differentiation-associated protein (Kdap), on human chromosome 19q13.1. These gene products appeared to carry functional signal sequences. We then designated these two splicing variants as dermokine-alpha and -beta. Northern blotting and quantitative RT-PCR revealed that dermokine-alpha/-beta, suprabasin, and Kdap were highly expressed in stratified epithelia. In mouse embryonic development, dermokine-alpha/-beta began to be expressed during the period of stratification. Also, in differentiating primary cultured human keratinocytes, transcription of dermokine-alpha/-beta, suprabasin, and Kdap was induced. These findings indicated that dermokine-alpha/-beta, suprabasin, and Kdap are secreted from the spinous layer of the stratified epithelia and that these genes form a novel gene complex on the chromosome.
Matsui
Takeshi
T
KAN Research Institute, Inc., Shimogyo-ku, Kyoto 600-8815, Japan.
Hayashi-Kisumi
Fumie
F
Kinoshita
Yoko
Y
Katahira
Sayaka
S
Morita
Kazumasa
K
Miyachi
Yoshiki
Y
Ono
Yuichi
Y
Imai
Toshio
T
Tanigawa
Yoko
Y
Komiya
Tohru
T
Tsukita
Shoichiro
S
D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D003001:Cloning, Molecular; D004817:Epidermis / Q000378:metabolism; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D000943:Antigens, Differentiation; C495568:DMKN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; C495569:dermokine protein, mouse; C470006:suprabasin protein, mouse; D016282:Aspartic Acid Endopeptidases; C104073:Kdap protein, mouse