title: Effects of recombinant cholera toxin b subunit (rCTB) on cellular immune responses: enhancement of delayed-type hypersensitivity following intranasal co-administration of Mycobacterium bovis-BCG with rCTB.
abstract: Recombinant cholera toxin B subunit (rCTB) is a safe and potent mucosal adjuvant. To gain insight into the mechanism underlying the adjuvant effect of rCTB, the effects of rCTB on cell-mediated immune responses of mice and guinea pigs were examined after intranasal administration of Mycobacterium bovis -bacillus Calmette-Guérin (BCG) with and without rCTB. Delayed-type hypersensitivity, for skin reactions in guinea pigs and for footpad swelling reactions in mice, to purified protein derivative (PPD) were enhanced by intranasal co-administration of BCG and rCTB, as compared to giving BCG alone to these animals. Moreover, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma production of spleen cells and antigen specific spleen cell proliferation, stimulated with PPD, were enhanced in the presence of rCTB. These results strongly suggest that rCTB enhances cellular as well as humoral immune responses.
authors.affiliation: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan. meymj@nih.go.jp
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Maeyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Isaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yasuda
authors.forename: Keiko
authors.initials: K
authors.lastname: Matano
authors.forename: Kazunori
authors.initials: K
authors.lastname: Morokuma
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohkuma
authors.forename: Kunio
authors.initials: K
authors.lastname: Tochikubo
authors.forename: Saburo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Norihisa
authors.initials: N
authors.lastname: Goto
mesh_terms: D000818:Animals; D002455:Cell Division; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000209:etiology*; D007114:Immunization / Q000662:veterinary*; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014373:Tuberculin / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D014373:Tuberculin; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D002772:Cholera Toxin