The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion.
The effect of a recombinant RGD (arginine-glycine-aspartic acid)-containing fusion protein, cellulose-binding domain (CBD)-RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum-free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD-RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly(lactide-co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface.
Hsu
Shan-Hui
SH
Department of Chemical Engineering, National Chung Hsing University, 250 Kao Kung Road, Taichung 402, Taiwan. shhsu@nchu.edu.tw
Chu
Wen-Ping
WP
Lin
Yu-Shuen
YS
Chiang
Yu-Lin
YL
Chen
David Chanhen
DC
Tsai
Ching-Lin
CL
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology*; D010865:Pilot Projects; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; D011993:Recombinant Fusion Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); C047981:arginyl-glycyl-aspartic acid; D002482:Cellulose