title: ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry.
abstract: ProSight PTM (https://prosightptm.scs.uiuc.edu/) is a web application for identification and characterization of proteins using mass spectra data from 'top-down' fragmentation of intact protein ions (i.e. without any tryptic digestion). ProSight PTM has many tools and graphical features to facilitate analysis of single proteins, proteins in mixtures and proteins fragmented in parallel. Sequence databases from across the phylogenetic tree are supported, with a new database strategy of 'shotgun annotation' used to assist characterization of wild-type proteins. During a database search, data from divergent sources regarding potential mass differences such as polymorphisms, alternate splicing and post-translational modifications are utilized. The user can optionally control how much of this biological variability should be searched.
authors.affiliation: W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: LeDuc
authors.forename: Gregory K
authors.initials: GK
authors.lastname: Taylor
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Kim
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Januszyk
authors.forename: Lee H
authors.initials: LH
authors.lastname: Bynum
authors.forename: Joseph V
authors.initials: JV
authors.lastname: Sola
authors.forename: John S
authors.initials: JS
authors.lastname: Garavelli
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
mesh_terms: D017398:Alternative Splicing; D030562:Databases, Protein; D006801:Humans; D020407:Internet; D013058:Mass Spectrometry; D011110:Polymorphism, Genetic; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry; D020539:Sequence Analysis, Protein; D016324:Sequence Tagged Sites; D012984:Software; D014584:User-Computer Interface
chemical_list: D011506:Proteins; C486166:RPS28 protein, human; D012269:Ribosomal Proteins