Identification of rice genes induced in a rice blast-resistant mutant.
To clarify mechanisms of rice blast resistance in rice plants we used suppression subtractive hybridization (SSH) to isolate genes induced upon rice blast inoculation in a rice blast-resistant mutant. A total of 26 rice cDNAs were isolated and found to have elevated expression upon rice blast infection in a rice blast-resistant derivative, SHM-11, of the rice cultivar, Sanghaehyanghyella. Sequencing of the cDNAs revealed that many of the proteins they encoded had been previously described as involved in plant responses against pathogen attack. Two interesting groups of the defense-related proteins consisted of three different PR5 homologues and four different protease inhibitors, all highly expressed in the rice blast mutant. Genes encoding proteins involved in signal transduction and regulation were also identified, including translation initiation factor eIF5A, C2 domain DNA binding protein, putative rice EDS and putative receptor like kinase. Most of the identified cDNAs were highly expressed 24 h after blast inoculation. Our results suggest that a pathway regulating defense gene expression may be altered in the mutant, resulting in early induction of the defense genes upon fungal infection.
Han
Chong U
CU
Biological Function Research Team, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea.
Lee
Chan Hui
CH
Jang
Kyoung Soo
KS
Choi
Gyung Ja
GJ
Lim
He Kyoung
HK
Kim
Jin-Cheol
JC
Ahn
Sang-Nag
SN
Choi
Jae Eul
JE
Cha
Jae Soon
JS
Kim
Heung Tae
HT
Cho
Kwang Yun
KY
Lee
Seon-Woo
SW
D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics; D020082:Magnaporthe / Q000472:pathogenicity; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D000097574:Eukaryotic Translation Initiation Factor 5A
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D000097574:Eukaryotic Translation Initiation Factor 5A; C098755:PR5K protein, Arabidopsis