title: Phosphorylation by protein kinase CK2 modulates the activity of the ATP binding cassette A1 transporter.
abstract: In a previous characterization of the ABCA subfamily of the ATP-binding cassette (ABC) transporters, we identified potential protein kinase 2 (CK2) phosphorylation sites, which are conserved in eukaryotic and prokaryotic members of the ABCA transporters. These phosphorylation residues are located in the conserved cytoplamic R1 and R2 domains, downstream of the nucleotide binding domains NBD1 and NBD2. To study the possible regulation of the ABCA1 transporter by CK2, we expressed the recombinant cytoplasmic domains of ABCA1, NBD1+R1 and NBD2+R2. We demonstrated that in vitro ABCA1 NBD1+R1, and not NBD2+R2, is phosphorylated by CK2, and we identified Thr-1242, Thr-1243, and Ser-1255 as the phosphorylated residues in the R1 domain by mass spectrometry. We further investigated the functional significance of the threonine and serine phosphorylation sites in NBD1 by site-directed mutagenesis of the entire ABCA1 followed by transfection into Hek-293 Tet-Off cells. The ABCA1 flippase activity, apolipoprotein AI and AII binding, and cellular phospholipid and cholesterol efflux were enhanced by mutations preventing CK2 phosphorylation of the threonine and serine residues. This was confirmed by the effect of specific protein kinase CK2 inhibitors upon the activity of wild type and mutant ABCA1 in transfected Hek-293 Tet-Off cells. The activities of the mutants mimicking threonine phosphorylation were close to that of wild type ABCA1. Our data, therefore, suggest that besides protein kinase A and C, protein kinase CK2 might play an important role in vivo in regulating the function and transport activity of ABCA1 and possibly of other members of the ABCA subfamily.
authors.affiliation: Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, B-9000 Ghent, Belgium. stein.roosbeek@UGent.be
authors.forename: Stein
authors.initials: S
authors.lastname: Roosbeek
authors.forename: Frank
authors.initials: F
authors.lastname: Peelman
authors.forename: Annick
authors.initials: A
authors.lastname: Verhee
authors.forename: Christine
authors.initials: C
authors.lastname: Labeur
authors.forename: Hans
authors.initials: H
authors.lastname: Caster
authors.forename: Marc F
authors.initials: MF
authors.lastname: Lensink
authors.forename: Claudia
authors.initials: C
authors.lastname: Cirulli
authors.forename: Johan
authors.initials: J
authors.lastname: Grooten
authors.forename: Claude
authors.initials: C
authors.lastname: Cochet
authors.forename: Joël
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
authors.forename: Giovanna
authors.initials: G
authors.lastname: Chimini
authors.forename: Jan
authors.initials: J
authors.lastname: Tavernier
authors.forename: Maryvonne
authors.initials: M
authors.lastname: Rosseneu
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D047390:Casein Kinase II / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010743:Phospholipids / Q000378:metabolism; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D010743:Phospholipids; D011994:Recombinant Proteins; D002784:Cholesterol; D047390:Casein Kinase II