[Assessment on effect of short-term cryopreservation of cord blood hematopoietic cells].
To study the effects of short-term cryopreservation of cord blood hematopoietic cells in liquid nitrogen, the viability and function of cord blood hematopoietic cells were investigated by using each of 8 samples cryopreserved for six months, one and two years after thawing respectively. Nucleated cells (NC) were detected by blood cell analyzer. CD34+ cells were analyzed by flow cytometry, CFU-GM were cultured and detected in vitro, the survival rate was determined by trypan blue staining. The results showed that the differences of recovery rate of NC, CD34+, CFU-GM were nonsignificant at three different cryopreserved times. In conclusion, the short-term storage in liquid nitrogen showed a good effect on cord blood hematopoietic cell without any significant change of activities and number of the cryopreserved hematopoietic cells.
He
Ji
J
Blood Centre of Zhejiang Province, Hangzhou 310006, China.
Liu
Jin-Hui
JH
Jiang
Kan
K
Zhu
Fa-Ming
FM
Yan
Li-Xing
LX
D002470:Cell Survival; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans