cDNA microarray analysis of rice anther genes under chilling stress at the microsporogenesis stage revealed two genes with DNA transposon Castaway in the 5'-flanking region.
Rice is most chilling sensitive at the onset of microspore release. Chilling treatment at this stage causes male sterility. The gene expression profile during the microspore development process under chilling stress was revealed using a microarray that included 8,987 rice cDNAs. As many as 160 cDNAs were up- or down-regulated by chilling during the microspore release stage. RT-PCR analysis of 5 genes confirmed the microarray results. We identified 3 novel genes whose expression levels were remarkably changed by chilling in rice anther. A new cis element that includes a DNA transposon Castaway sequence was found in the 5' upstream region of two genes which were conspicuously down-regulated by chilling temperatures in rice anther.
Yamaguchi
Tomoya
T
Plant Physiology Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, Japan. tomyamag@affrc.go.jp
Nakayama
Katsuhiro
K
Hayashi
Takami
T
Yazaki
Junshi
J
Kishimoto
Naoki
N
Kikuchi
Shoshi
S
Koike
Setsuo
S
D024506:5' Flanking Region / Q000235:genetics; D001483:Base Sequence; D003080:Cold Temperature; D004251:DNA Transposable Elements; D004742:Enhancer Elements, Genetic; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics
D004251:DNA Transposable Elements; D010940:Plant Proteins