title: Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma.
abstract: OBJECTIVE
  To analyze loss of heterozygosity (LOH) and homozygous deletion on p53 gene (exon2-3, 4 and 11), chromosome 10q22-10q23 and 22q11.2 -22q12.1 in human hepatocellular carcinoma (HCC).
  
  
  METHODS
  PCR and PCR-based microsatellite polymorphism analysis techniques were used.
  
  
  RESULTS
  LOH was observed at D10S579 (10q22-10q23) in 4 of 20 tumors (20%), at D22S421 (22q11.2-22q12.1) in 3 of 20(15%), at TP53.A (p53 gene exon 2-3) in 4 of 20(20%), at TP53.B (p53 gene exon 4) in 6 of 20(30%), and at TP53.G (p53 gene exon 11) in 0 of 20(0%). Homozygous deletion was detected at 10q22-10q23(8/20; 40%), 22q11.2-22q12.1(8/20; 40%), p53 gene exon 2-3(0/20;0%), p53 gene exon 4(6/20; 30%), and p53 gene exon 11(2/20; 10%).
  
  
  CONCLUSIONS
  There might be unidentified tumor suppressor genes on chromosome 10q22-10q23 and 22q11.2-22q12.1 that contribute to the pathogenesis and development of HCC.
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Guang-Neng
authors.initials: GN
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Zuo
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Su-Mei
authors.initials: SM
authors.lastname: Zhang
authors.forename: Hua-Qing
authors.initials: HQ
authors.lastname: Zhu
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Gui
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D005091:Exons; D017353:Gene Deletion; D006720:Homozygote; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53