Multiserotype protection of mice against pneumococcal colonization of the nasopharynx and middle ear by killed nonencapsulated cells given intranasally with a nontoxic adjuvant.
Intranasal challenge of C57BL/6 mice with Streptococcus pneumoniae serotypes 6B, 14, and 23F produced colonization of the middle ear and NP. Intranasal vaccination with ethanol-killed nonencapsulated cells with adjuvant protected both sites. Of four nontoxic adjuvants tested, the cholera toxin B subunit was most effective and least nonspecifically protective.
Malley
Richard
R
Division of Infectious Diseases, Children's Hospital, 300 Longwood Ave., Boston, MA 02115, USA. richard.malley@childrens.harvard.edu
Morse
Sarah C
SC
Leite
Luciana C C
LC
Areas
Ana Paula Mattos
AP
Ho
Paulo Lee
PL
Kubrusly
Flavia S
FS
Almeida
Igor C
IC
Anderson
Porter
P
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D004427:Ear Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D004432:Ear, Middle / Q000276:immunology / Q000382:microbiology; D051379:Mice; D009302:Nasopharyngeal Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D011008:Pneumococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000276:immunology* / Q000494:pharmacology; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000276:immunology*
D000276:Adjuvants, Immunologic; D022242:Pneumococcal Vaccines