Developmental expression of OAP-1/Tspan-3, a member of the tetraspanin superfamily.
OSP/claudin-11-associated protein (OAP-1/Tspan-3), originally isolated by yeast two-hybrid screening using OSP/claudin-11 (oligodendrocyte-specific protein) as bait, is a member of the tetraspanin superfamily and OAP-1/Tspan-3, OSP/claudin-11, and beta1 integrin form a protein complex that seems to be involved in oligodendrocyte proliferation and migration. This study investigated the temporal and regional expression, glycosylation status, and tissue distribution of OAP-1/Tspan-3. OAP-1/Tspan-3 mRNA was expressed as a single transcript throughout brain development, with high levels of expression in the germinal zones. OAP-1/Tspan-3 protein contains N-terminal glycosylation sites in extracellular loop 2 and deglycosylation studies indicated a decrease in apparent molecular weight of OAP-1/Tspan-3, consistent with removal of N-glycans. Similar to OSP/claudin-11, OAP-1/Tspan-3 is expressed in all stages of oligodendrocyte development and in the myelin sheath. Unlike OSP/claudin-11, however, it is expressed in all cell types tested in the central nervous system (CNS), including neurons and astrocytes. The association of OAP-1/Tspan-3 with OSP/claudin-11 and beta1 integrin, its subcellular distribution as a cell surface, membrane-spanning glycoprotein, and its widespread distribution supports its potential role in cell migration, proliferation, and interactions between cells and extracellular matrix.
Tiwari-Woodruff
Seema K
SK
Departments of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.
Kaplan
Roman
R
Kornblum
Harley I
HI
Bronstein
Jeff M
JM
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D002450:Cell Communication / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D057167:Claudins; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006031:Glycosylation; D019012:Integrin beta1 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D059470:Tetraspanins
D057167:Claudins; C492354:Cldn11 protein, mouse; C492353:Cldn11 protein, rat; D019012:Integrin beta1; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D059470:Tetraspanins; C426223:Tspan3 protein, mouse; C491834:Tspan3 protein, rat