title: [Effect of cigarette smoke solution on viability of rat lymphocytes and the intervention of antioxidants].
abstract: OBJECTIVE
  To learn the effect of cigarette smoke solution (CSS) on viability of rat lymphocytes and the intervention of antioxidants.
  
  
  METHODS
  CSS was prepared by bubbling main-stream cigarette smoke into PBS, then 0.1 x 10(-3) cig/ml, 2 x 10(-3) cig/ml, 4 x 10(-3) cig/ml, 8 x 10(-3) cig/ml, 12 x 10(-3) cig/ml, 16 x 10(-3) cig/ml CSS were added into 7 groups of rat lymphocytes respectively. Alamar blue was added to each group 2 hours later, and after another 4 hours reduction rate of Alamar blue was detected and used to evaluate viability of cells. Otherwise, pretreated with vitamin C (VC), vitamin E(VE), glutathione(GSH), melatonin(MLT) or dimethyl sulfoxide(DMSO), cells were treated with 8 x 10(-3) cig/ml CSS and reduction rates of Alamar blue were detected as before.
  
  
  RESULTS
  Alamar blue reduction rates in 2 x 10(-3) cig/ml - 16 x 10(-3) cig/ml groups were lower than that of control significantly, and there was a dose-effect relationship. Also, Alamar blue reduction rates increased in CSS-groups pretreated with 0.05 mmol/LVC, 0.2 mmol/LVE, 0.1 mmol/LMLT, 1 mmol/LGSH or 1% DMSO.
  
  
  CONCLUSIONS
  Viability of rat lymphocytes decreased in the presence of CSS, and it could be antagonised by antioxidants such as vitamin C, vitamin E, glutathione, melatonin and dimethyl sulfoxide.
authors.affiliation: School of Public Health, China Medical University, Shenyang 110001, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
authors.forename: Chao
authors.initials: C
authors.lastname: Wan
authors.forename: Xiance
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D008550:Melatonin; D001205:Ascorbic Acid; D004121:Dimethyl Sulfoxide