title: Early detection of alterations associated to oral cancer.
abstract: The model of hamster cheek pouch carcinogenesis closely mimics the development of human oral cancer. The study of the interaction between chemical carcinogens and radiation in the process of oral carcinogenesis is of interest given that the oral cavity is frequently exposed to chemical carcinogens such as alcohol and tobacco and is the route of entry of therapeutic radiation. In this context, markers of incipient alterations associated to a process of malignant transformation would contribute to early diagnosis and follow-up. The aim of the present study was to assess the early changes produced by carcinogenic agents applied separately or combined in a two-stage carcinogenesis protocol in hamster cheek pouch. The cheek pouch of the hamsters was treated with a single dose of radiation (20 Gy) or 7,12-dimethylbenz(a)anthracene (DMBA) as initiating agents and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoting agent for 1 or 2 weeks. The end-points chosen to identify early alterations were hyperplastic foci and silver-stained nucleolar organizer regions (Ag NOR). The data show that both markers are useful in the detection of early alterations compatible with a process of malignant transformation.
authors.affiliation: Department of Radiobiology, National Atomic Energy Commission, Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires.
authors.forename: S A
authors.initials: SA
authors.lastname: Chimeno Zoth
authors.forename: A M
authors.initials: AM
authors.lastname: Collet
authors.forename: E
authors.initials: E
authors.lastname: Heber
authors.forename: A E
authors.initials: AE
authors.lastname: Schwint
authors.forename: M E
authors.initials: ME
authors.lastname: Itoiz
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000704:Analysis of Variance; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000175:diagnosis*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D002610:Cheek; D006224:Cricetinae; D006965:Hyperplasia; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000175:diagnosis*; D009381:Neoplasms, Radiation-Induced / Q000175:diagnosis*; D009697:Nucleolus Organizer Region / Q000473:pathology*; D016622:Silver Staining; D018709:Statistics, Nonparametric; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate