Constitutive activation of the PDR16 promoter in a Candida albicans azole-resistant clinical isolate overexpressing CDR1 and CDR2.
Candida albicans azole-resistant clinical isolates overexpressing the CDR1 and CDR2 genes (multidrug transporters) also overexpress the PDR16 gene (phosphatidylinositol transfer protein). We show here that the PDR16 promoter displays higher transcriptional activity following integration in an azole-resistant isolate than in the matched azole-susceptible one. Thus, the upregulation of PDR16 in the resistant strain results from a mutation acting in trans.
De Deken
Xavier
X
Institut de Recherches Cliniques de Montréal, 110 Pine Avenue West, Montréal, Québec, Canada H2W 1R7.
Raymond
Martine
M
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects*; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D030541:Databases, Genetic; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D049294:Phospholipid Transfer Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D000935:Antifungal Agents; D001393:Azoles; C094693:CDR1 protein, Candida albicans; D002352:Carrier Proteins; D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D049294:Phospholipid Transfer Proteins