Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.
We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.
Kamionka
Annette
A
Lehrstuhl für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
Sehnal
Miriam
M
Scholz
Oliver
O
Hillen
Wolfgang
W
D043364:Endo-1,4-beta Xylanases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D013752:Tetracycline / Q000494:pharmacology*; D001617:beta-Glucosidase / Q000235:genetics
D029968:Escherichia coli Proteins; C470187:TetR(B) protein, E coli; D001617:beta-Glucosidase; D043364:Endo-1,4-beta Xylanases; C090001:XynB xylanase; D013752:Tetracycline