Comparative characterization of the oah2 gene homologous to the oah1 of Thermus thermophilus HB8.
The oah2 gene homologous to the oah1 of Thermus thermophilus HB8 was cloned and sequenced. It comprised 1,236 bp encoding a protein of 412 amino acid residues and was overexpressed. The gene product, also having O-acetyl-L-homoserine sulfhydrylase (EC 4.2.99.10) activity, was purified to homogeneity and characterized comparatively with the oah1 product. The two proteins shared many characteristics.
Iwama
Tomonori
T
Department of Biotechnology, Faculty of Agriculture, Gifu University. iwamatom@cc.gifu-u.ac.jp
Hosokawa
Hideo
H
Lin
Weimin
W
Shimizu
Hideaki
H
Kawai
Keiichi
K
Yamagata
Shuzo
S
D000595:Amino Acid Sequence; D019757:Carbon-Oxygen Lyases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D006863:Hydrogen-Ion Concentration; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics
C018725:O-acetylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases