Chamber with modifiable inner width for performing transversal alternating field electrophoresis in variable numbers of minigels.
We present a transversal alternating field electrophoresis chamber that allows modifiable inner widths to accommodate low- or high-throughput formats, with 7.8 cm opposite electrode separation and 30.4 cm electrode length. Removable slotted sheets divide the chamber into four smaller compartments, each one supporting a minigel of 3.85 cm in length and 7.1 cm in width. Replacements of slotted sheets with solid dielectric blocks with the sizes and shapes of compartments permit to occlude chamber compartments, changing from 4 to 1 the numbers of minigels per run, from 88 to 13 the maximum numbers of samples, and from 1688 to 422 mL the volume of buffer poured into the chamber. Saccharomyces cerevisiae chromosomes gave its characteristic DNA band pattern in all compartments, whereas migrations of DNA molecules are not affected by the occlusion of compartments.
Riverón
Ana María
AM
Department of Molecular Biology, Cuban Center for Neurosciences (CNC), National Center for Scientific Research (CNIC), Havana, Cuba. anam@cneuro.edu.cu
Herrera
José Alfredo
JA
López-Cánovas
Lilia
L
D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal