Detection of circulating antigens in experimental anisakiasis by two-site enzyme-linked immunosorbent assay.
A two-site enzyme-linked immunosorbent assay (ELISA) based on the avidin-biotin system was developed to study circulating antigens in the sera of rats orally or intraperitoneally infected with L3 larvae of Anisakis simplex s.l. Somatic and excretory-secretory antigens were detected from 24 h post-infection in all infected rats. A direct relationship between the number of larvae inoculated and the concentration of antigens in the sera was demonstrated; however, the amount of antigen decreased with the course of the infection. Comparative analysis revealed that intraperitoneal inoculation is most appropriate for detecting excretory-secretory antigens in serum. The minimum concentrations of A. simplex antigen detectable were approximately 2.5 microg/ml for somatic and excretory-secretory antigens. This antigen detection assay may have application in the diagnosis of anisakiasis.
Campos
M
M
Departamento de Parasitología, Facultad de Farmacia, Universidad de Granada, Campus Universitario Cartuja, 18071, Spain.
Martín
L
L
Díaz
V
V
Mañas
I
I
Morales
B
B
Lozano
J
J
D000818:Animals; D017129:Anisakiasis / Q000175:diagnosis / Q000469:parasitology*; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D007814:Larva / Q000276:immunology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth