title: Cellulose-based chromatography for cellooligosaccharide production.
abstract: The potential of using cellulose stationary phases for the chromatographic fractionation of cellooligosaccharide preparations has been explored. The impetus for the work is the current interest in using cellooligosaccharides as functional nondigestible oligosaccharides in foods. The conceptual studies illustrate the potential of using ethanol-water mobile phases in conjunction with cellulose stationary phases for cellooligosaccharide fractionation. Cellooligosaccharide solubility in ethanol-water mixtures and their elution order from cellulose-based columns using ethanol-water mobile phases were shown to be in line with their degree of polymerization (DP), with the higher DP cellooligosaccharides being less soluble and having longer retention times. The retention volume for all COS increased with increased temperature. Both microcrystalline and fibrous cellulose preparations were shown to work as chromatographic stationary phases. The application experiments demonstrate the potential of using cellulose stationary phases for the cleanup and fractionation of cellooligosaccharide mixtures generated via acid-catalyzed hydrolysis of cellulose.
authors.affiliation: Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331-660, USA. akpinar99@yahoocom
authors.forename: Ozlem
authors.initials: O
authors.lastname: Akpinar
authors.forename: Robert J
authors.initials: RJ
authors.lastname: McGorrin
authors.forename: Michael H
authors.initials: MH
authors.lastname: Penner
mesh_terms: D002482:Cellulose / Q000008:administration & dosage / Q000737:chemistry; D005591:Chemical Fractionation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D004355:Drug Stability; D000431:Ethanol / Q000008:administration & dosage; D006358:Hot Temperature; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D009844:Oligosaccharides; D000431:Ethanol; D002482:Cellulose