Pancreatic islets isolation using different protocols with in situ flushing and intraductal collagenase injection.
Human islet transplantation seems to be a very promising clinical procedure for patients with type I diabetes mellitus. The aim of our study was to investigate the influence of in situ intravascular flushing with University of Wisconsin (UW) solution and intraductal collagenase injection at the time of pancreas procurement on the isolated islets and exocrine tissue injury. Our experiments indicated that in situ perfusion with the UW solution has a beneficial effect on pancreatic islets and intraductal distention results in an increase in the concentration of pancreatic enzymes released into the cold preservation solution during ischemic conditions. Cold ischemia reduced islet yield, but pancreas perfusion with the UW solution showed better ischemic tolerance of isolated islets during glucose static incubation. We conclude that intravascular pancreas flushing has a crucial effect on recovery and yield of pancreatic islets and protects against exocrine tissue injury.
Kinasiewicz
A
A
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland. kinas@ibib.waw.pl
Juszczak
M
M
Pachecka
J
J
Fiedor
P
P
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D017364:Collagenases / Q000008:administration & dosage*; D007267:Injections; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D020858:Tissue and Organ Harvesting / Q000379:methods*
D017364:Collagenases