Induction of apoptosis by Antrodia camphorata in human premyelocytic leukemia HL-60 cells.
Antrodia camphorata (A. camphorata) is well known in Taiwan as a traditional Chinese medicine, and it has been shown to exhibit antioxidant effects. In this study, the ability of A. camphorata to induce apoptosis was studied in cultured human premyelocytic leukemia HL-60 cells. Treatment of the HL-60 cells with a variety of concentrations of the fermented culture broth of A. camphorata (25-150 microg/ml) resulted in dose- and time-dependent sequences of events marked by apoptosis, as shown by loss of cell viability, chromatin condensation, and internucleosomal DNA fragmentation. Furthermore, apoptosis in the HL-60 cells was accompanied by the release of cytochrome c, activation of caspase-3, and specific proteolytic cleavage of poly (ADP-ribose) polymerase (PARP). This increase in A. camphorata-induced apoptosis was also associated with a reduction in the levels of Bcl-2, a potent cell-death inhibitor, and an increase in those of the Bax protein, which heterodimerizes with and thereby inhibits Bcl-2. The data suggest that A. camphorata exerts antiproliferative action and growth inhibition on HL-60 cells through apoptosis induction and that it may have anticancer properties valuable for application in drug products.
Hseu
You-Cheng
YC
Department of Food Science, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan.
Yang
Hsin-Ling
HL
Lai
Yu-Ching
YC
Lin
Jaung-Geng
JG
Chen
Guan-Wei
GW
Chang
Yung-Hsien
YH
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019254:Genes, bcl-2 / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D007700:Kinetics; D011124:Polyporaceae / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases