Localization of alkaline phosphatase and proteins related to intercellular junctions in rat hepatoma cell line McA-RH 7777.
We investigated the localization of alkaline phosphatase (ALP) and three proteins related to intercellular junctions in the McA-RH 7777 rat hepatoma cell line to determine if the formation of junctions between adjacent McA-RH 7777 cells triggers translocation of ALP from cytoplasm to the plasma membrane. Contact between adjacent McA-RH 7777 cells promotes translocation of ALP from the Golgi area of the cytoplasm to the plasma membrane, and also promotes translocation of two proteins, E-cadherin and ZO-1, related to intercellular junctions, from cytoplasm to the plasma membrane.
Chida
Kohsuke
K
Department of Anatomy, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan. chida@ahs.kitasato-u.ac.jp
Taguchi
Meiko
M
D022005:Adherens Junctions / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D017630:Connexins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism; D007365:Intercellular Junctions / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D019108:Tight Junctions / Q000378:metabolism; D062826:Zonula Occludens-1 Protein; D000097002:Gap Junction beta-1 Protein
D000469:Alkaline Phosphatase; D015820:Cadherins; D017630:Connexins; D008565:Membrane Proteins; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein; D000097002:Gap Junction beta-1 Protein; C569044:Tjp1 protein, rat