Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.
The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20 kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His(5) tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 A. A complete 2.0 A data set has been collected at a rotating-anode X-ray source and structure determination is under way.
Ren
Hui
H
Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Liang
Yuhe
Y
Li
Rui
R
Ding
Haitao
H
Qiu
Shihong
S
Lu
Shanyun
S
An
Jianli
J
Li
Lanfen
L
Luo
Ming
M
Zheng
Xiaofeng
X
Su
Xiao Dong
XD
D000311:Adrenal Glands / Q000737:chemistry*; D002853:Chromatography, Liquid; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID
D011506:Proteins; C106849:TAF9 protein, human; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID