Binding of salivary agglutinin to IgA.
SAG (salivary agglutinin), which is identical to gp-340 (glycoprotein-340) from the lung, is encoded by DMBT1 (deleted in malignant brain tumours 1). It is a member of the SRCR (scavenger receptor cysteine-rich) superfamily and contains 14 SRCR domains, 13 of which are highly similar. SAG in saliva is partially complexed with IgA, which may be necessary for bacterial binding. The goal of the present study was to characterize the binding of purified SAG to IgA. SAG binds to a variety of proteins, including serum and secretory IgA, alkaline phosphatase-conjugated IgGs originating from rabbit, goat, swine and mouse, and lactoferrin and albumin. Binding of IgA to SAG is calcium dependent and is inhibited by 0.5 M KCl, suggesting that electrostatic interactions are involved. Binding of IgA was destroyed after reduction of SAG, suggesting that the protein moiety is involved in binding. To pinpoint further the binding domain for IgA on SAG, a number of consensus-based peptides of the SRCR domains and SRCR interspersed domains were designed and synthesized. ELISA binding studies with IgA indicated that only one of the peptides tested, comprising amino acids 18-33 (QGRVEVLYRGSWGTVC) of the 109-amino-acid SRCR domain, exhibited binding to IgA. This domain is identical to the domain of SAG that is involved in binding to bacteria. Despite this similar binding site, IgA did not inhibit binding of Streptococcus mutans to SAG or peptide. These results show that the binding of IgA to SAG is specifically mediated by a peptide sequence on the SRCR domains.
Ligtenberg
Antoon J M
AJ
Department of Dental Basic Sciences, Section Oral Biochemistry, Academic Centre for Dentistry, Medical Faculty of the Free University, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. ajm.ligtenberg@vumc.nl
Bikker
Floris J
FJ
De Blieck-Hogervorst
Jolanda M A
JM
Veerman
Enno C I
EC
Nieuw Amerongen
Arie V
AV
D000373:Agglutinins / Q000737:chemistry / Q000276:immunology*; D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D002135:Calcium-Binding Proteins; D016384:Consensus Sequence; D004268:DNA-Binding Proteins; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000276:immunology*; D011971:Receptors, Immunologic / Q000737:chemistry*; D051116:Receptors, Scavenger; D016415:Sequence Alignment; D013295:Streptococcus mutans / Q000276:immunology; D025521:Tumor Suppressor Proteins
D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D007070:Immunoglobulin A; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D025521:Tumor Suppressor Proteins