title: [Effect of acrylonitrile on the spermatogenesis in mice].
abstract: OBJECTIVE
  To observe the effect of acrylonitrile on the spermatogenesis in mice.
  
  
  METHODS
  50 male pubertal mice and 25 male adult mice were exposed to acrylonitrile (1.25, 2.5, 5 mg/kg) by intraperitoneal injection for 5 days, and detected the DNA content and cell period of spermatogenic cells by flow cytometry after 35 days.
  
  
  RESULTS
  (1) The percentage of 1C cell in the exposed pubertal mice groups was lower than in the control group, respectively. The percentage of 1C cell in the exposed adult mice groups was lower than in the control group, respectively (P < 0.05). The percentage of apoptotic cell in pubertal and adult mice was higher than in the control group, respectively (P < 0.05). (2) The percentage of cells in S-phase in the exposed pubertal mice groups was higher than in the control group, respectively (P < 0.05), G0/G1 phase were lower(P < 0.05). The percentage of cells in G2/M phase in the exposed adult mice groups was higher than in the control group, respectively(P < 0.05), G0/G1 phase was lower(P < 0.05).
  
  
  CONCLUSIONS
  There are restrained effects of acrylonitrile on the spermatogenesis in mice.
authors.affiliation: School of Public Health, Lanzhou Medical College, Lanzhou 730000, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Wang
authors.forename: Suqin
authors.initials: S
authors.lastname: Lian
mesh_terms: D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004247:DNA / Q000032:analysis; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects
chemical_list: D004247:DNA; D000181:Acrylonitrile