Antagonistic interactions between the SA and JA signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to cucumber mosaic virus.
Gene-for-gene resistance to a yellow strain of cucumber mosaic virus [CMV(Y)] is conferred by the dominant RESISTANCE to CMV(Y) (RCY1) allele in the Arabidopsis thaliana ecotype C24. RCY1-conferred resistance to CMV(Y) and expression of the Pathogenesis-related 1 (PR-1) and PR-5 genes are partially compromised by the eds5 mutation and the nahG transgene that block accumulation of salicylic acid (SA). In contrast, the RCY1-conferred resistance to CMV(Y) is not affected by the jasmonic acid (JA)-insensitive coi1 and jar1 mutations. Interestingly, we report here that in contrast to the eds5 RCY1 plant, the eds5 coi1 RCY1 double-mutant plant exhibited a higher level of resistance to CMV(Y). Presence of the coi1 mutant allele also restored the CMV(Y)-activated expression of the PR-1 and PR-5 gene in the eds5 coi1 RCY1 plant. In contrast to the PR-1 and PR-5 genes, expression of the JA-dependent PLANT DEFENSIN 1.2 (PDF1.2) and HEVEIN-LIKE PROTEIN (HEL) genes was elevated in the CMV(Y)-inoculated leaves of the eds5 RCY1 plant, but not in the virus-inoculated leaves of the wild-type RCY1 and coi1 RCY1 plants. We propose that antagonistic interactions between the SA and JA signaling mechanisms modulate defense gene expression and the activation of RCY1-conferred gene-for-gene resistance to CMV(Y).
Takahashi
Hideki
H
Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan. takahash@bios.tohoku.ac.jp
Kanayama
Yoshinori
Y
Zheng
Ming Shu
MS
Kusano
Tomonobu
T
Hase
Shu
S
Ikegami
Masato
M
Shah
Jyoti
J
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D017799:Cucumovirus / Q000472:pathogenicity*; D003517:Cyclopentanes / Q000378:metabolism*; D023082:Defensins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000378:metabolism*; D000068103:Serine-Arginine Splicing Factors; D015398:Signal Transduction / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D010453:Peptide Synthases
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D023082:Defensins; D026901:Membrane Transport Proteins; D009713:Nucleotidyltransferases; D054883:Oxylipins; D020156:Salicylic Acid; D000068103:Serine-Arginine Splicing Factors; C112279:COI1 protein, Arabidopsis; C455514:EDS5 protein, Arabidopsis; C487542:At5g44420 protein, Arabidopsis; C473790:RCY1 protein, Arabidopsis; C077262:PR-1 protein, Arabidopsis; C077264:PR-5 protein, Arabidopsis; C011006:jasmonic acid; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases