Comparative analysis of plant and animal calcium signal transduction element using plant full-length cDNA data.
We obtained 32K full-length cDNA sequence data from the rice full-length cDNA project and performed a homology search against NCBI GenBank data. We have also searched homologs of Arabidopsis and other plants' genes with the databases. Comparative analysis of calcium ion transport proteins revealed that the genes specific for muscle and nerve calcium signal transduction systems (VDCC, IP3 receptor, ryanodine receptor) are very different in animals and plants. In contrast, Ca elements with basic functions in cell responses (CNGC, iGlu receptor, Ca(2+)ATPase, Ca2+/Na(+)-K+ ion exchanger) are basically conserved between plants and animals. We also performed comparative analyses of calcium ion binding and/or controlling signal transduction proteins. Many genes specific for muscle and nerve tissue do not exist in plants. However, calcium ion signal transduction genes of basic functions of cell homeostasis and responses were well conserved; plants have developed a calcium ion interacting system that is more direct than in animals. Many species of plants have specifically modified calcium ion binding proteins (CPK, CRK), Ca2+/phospholipid-binding domains, and calcium storage proteins.
Nagata
Toshifumi
T
Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannon dai, Tsukuba, Ibaraki, 305-8602 Japan.
Iizumi
Shigemi
S
Satoh
Kouji
K
Ooka
Hisako
H
Kawai
Jun
J
Carninci
Piero
P
Hayashizaki
Yoshihide
Y
Otomo
Yasuhiro
Y
Murakami
Kazuo
K
Matsubara
Kenichi
K
Kikuchi
Shoshi
S
D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary; D030562:Databases, Protein; D004705:Endocytosis / Q000235:genetics / Q000502:physiology; D006801:Humans; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology
D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D019255:NADPH Oxidases; D003546:Cysteine Endopeptidases; D002118:Calcium