Occupational exposure to aromatic hydrocarbons at a coke plant: Part I. Identification of hydrocarbons in air and their metabolites in urine by a gas chromatography-mass spectrometry method.
A method for the qualitative analysis of aromatic hydrocarbons in air and their various urinary metabolites is presented. The air was sampled in charcoal tubes and extracted with carbon disulfide. The hydrocarbons were identified as being aliphatic hydrocarbons (C(9)-C(19)), aromatic hydrocarbons and heterocyclic compounds. The urinary metabolites after enzymatic hydrolysis were analyzed by solid-phase extraction with a styrene-divinylbenzene resin, silylation with N,O-bis(trimethylsilyl)acetamide and GC/MS for separation and detection. Satisfactory separation of all compounds investigated was achieved without interference due to matrix peaks. The following compounds were identified in the urine of workers: dimethylphenol isomers, 4-ethyl-1,3-benzenediol, 2-ethoxybenzoic acid and methoxyphenols. Trimethylsilyl derivatives of aromatic hydroxyacids and hydroxymethoxyacids were found in the urine of occupationally exposed workers by means of a silylation procedure.
Bieniek
Grazyna
G
Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Sosnowiec, Poland. maja@slam.katowice.pl
Kurkiewicz
Slawomir
S
Wilczok
Tadeusz
T
D000328:Adult; D000393:Air Pollutants / Q000302:isolation & purification / Q000652:urine*; D001554:Benzene / Q000302:isolation & purification; D003077:Coke; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006571:Heterocyclic Compounds / Q000302:isolation & purification / Q000652:urine; D006801:Humans; D006840:Hydrocarbons, Alicyclic / Q000302:isolation & purification / Q000652:urine; D006841:Hydrocarbons, Aromatic / Q000302:isolation & purification / Q000652:urine*; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000652:urine; D008875:Middle Aged; D009281:Naphthalenes / Q000302:isolation & purification / Q000652:urine; D016273:Occupational Exposure / Q000032:analysis*; D014050:Toluene / Q000302:isolation & purification / Q000652:urine; D014992:Xylenes / Q000302:isolation & purification / Q000652:urine
D000393:Air Pollutants; D003077:Coke; D006571:Heterocyclic Compounds; D006840:Hydrocarbons, Alicyclic; D006841:Hydrocarbons, Aromatic; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D009281:Naphthalenes; D014992:Xylenes; C029266:2-ethoxybenzoic acid; C031721:naphthalene; D014050:Toluene; D001554:Benzene