Studies on experimental iodine allergy: 1. Antigen recognition of guinea pig anti-iodine antibody.
It has generally been thought that iodine allergy is cross-sensitive to various iodine-containing chemicals. However, this concept seems to deviate from the immunological principle that immune recognition is specific. To solve this contradiction, we hypothesize that iodine allergy is an immunological reaction to iodinated autologous proteins produced in vivo by iodination reaction from various iodine-containing chemicals. Antisera to iodine were obtained from guinea pigs immunized subcutaneously with iodine-potassium iodide solution emulsified in complete Freund's adjuvant (CFA). The specificity of guinea pig anti-iodine antiserum was determined by enzyme-linked immunosorbent assay (ELISA) inhibition experiments using microplates coated with iodinated guinea pig serum albumin (I-GSA). Antibody activities were inhibited by I-GSA, diiodo-L-tyrosine, and thyroxine, but not by potassium iodide, monoiodo-L-tyrosine, 3,5,3'-triiodothyronine, monoiodo-L-histidine, or diiodo-L-histidine, or by ionic or non-ionic iodinated contrast media. The results that antigen recognition of anti-iodine antibody is specific to iodinated protein support our hypothesis. While protein iodination usually takes place both at histidine residues as well as at tyrosine residues, only iodinated tyrosine acted as an antigenic determinant and no antibody activities to iodinated histidine were detected in our experimental iodine allergy model.
Shionoya
Hiroshi
H
Department of Drug Safety Research, Eisai Kawashima Research Laboratories, Eisai Co., Ltd., 1 Takehaya-cho, Kawashima-cho, Hashima-gun, Gifu 501-0061, Japan.
Sugihara
Yoshiki
Y
Okano
Kazuo
K
Sagami
Fumio
F
Mikami
Takashi
T
Katayama
Kouichi
K
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D004105:Diiodotyrosine / Q000009:adverse effects / Q000276:immunology; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D011193:Potassium Iodide / Q000009:adverse effects / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology; D013974:Thyroxine / Q000009:adverse effects / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D012709:Serum Albumin; D011193:Potassium Iodide; D004105:Diiodotyrosine; D005620:Freund's Adjuvant; D013974:Thyroxine