title: Molecular investigation of the stromal cell-derived factor-1 chemokine in lymphoid leukemia and lymphoma patients from Brazil.
abstract: The stromal cell-derived factor-1 (SDF-1) gene contains a common polymorphism, termed SDF1-3'A, in an evolutionarily conserved segment of the 3'-untranslated region (3'-UTR). We compared SDF-1 genotypes in patients diagnosed with lymphoid leukemias and lymphomas. Since the SDF1-3'A variant deletes the MspI restriction site, PCR-restriction fragment length polymorphism (RFLP) analysis was used for identification of genotypes. We identified the heterozygous genotype (3'A/wt) in 38.8% (24/62) of lymphoma patients and in 26.2% (11/42) of lymphoid leukemias. The percentage of 3'A carriers was significantly higher in lymphomas (43.5%) than in lymphoid leukemias (26.2%; P < 0.05). Our study indicates that lymphoma patients from Brazil are more likely to carry the 3'A gene than patients with lymphoid leukemias, suggesting that this polymorphism may be a differential determinant of lymphomas and lymphoid leukemia.
authors.affiliation: Department of Pathological Sciences, Londrina State University, Londrina, PR, Brazil.
authors.forename: Gabriela Gonçalves
authors.initials: GG
authors.lastname: de Oliveira Cavassin
authors.forename: Fernando Luiz
authors.initials: FL
authors.lastname: De Lucca
authors.forename: Nayara
authors.initials: N
authors.lastname: Delgado André
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Pelegrinelli Fungaro
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Voltarelli
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D002648:Child; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006801:Humans; D007945:Leukemia, Lymphoid / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D008223:Lymphoma / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D008297:Male; D017720:Molecular Epidemiology; D011110:Polymorphism, Genetic
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC