title: Isodon japonicus inhibits mast cell-mediated immediate-type allergic reactions.
abstract: The effect of aqueous extract of Isodon japonicus Hara (Labiatae) (IJAE) on mast cell-mediated immediate-type allergic reactions was investigated. IJAE inhibited systemic allergic reaction induced by compound 48/80. When IJAE was pretreated at the same concentrations with systemic allergic reaction test, the plasma histamine levels were reduced in a dose-dependent manner. IJAE also inhibited local allergic reaction activated by anti-dinitrophenyl (DNP) IgE antibody. IJAE dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. IJAE inhibited the secretion of tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated human mast cell line (HMC-1) cells. In addition, the expression of TNF-alpha mRNA in HMC-1 cells was inhibited by IJAE. These results indicate that IJAE may be beneficial in the regulation of immediate-type allergic reaction.
authors.affiliation: College of Agriculture and Sciences, Chonbuk National University, Jeonju, Jeonbuk, South Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Yong-Gil
authors.initials: YG
authors.lastname: Choi
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Shin
authors.forename: Moon-Kyu
authors.initials: MK
authors.lastname: Ahn
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000517:prevention & control*; D015850:Interleukin-6 / Q000378:metabolism; D037602:Isodon; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000633:toxicity
chemical_list: D015850:Interleukin-6; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003189:p-Methoxy-N-methylphenethylamine