title: Evolutionary and functional relationships among the nontypeable Haemophilus influenzae HMW family of adhesins.
abstract: Nontypeable Haemophilus influenzae (NTHi) is a common cause of localized respiratory tract disease and initiates infection by colonizing the nasopharynx. Approximately 75 to 80% of NTHi clinical isolates produce proteins that belong to the HMW family of adhesins, which are believed to facilitate colonization. The prototype HMW adhesins are designated HMW1 and HMW2 and were identified in NTHi strain 12. HMW1 and HMW2 are 71% identical and 80% similar overall, yet display differing cellular binding specificities. In the present study we set out to define more clearly the relationships between HMW1 and HMW2 and other members of the HMW family of adhesins. PCR analysis of 49 epidemiologically distinct isolates revealed that all strains possessing hmw genes as determined by Southern analysis contain two hmw loci in conserved, unlinked physical locations on the chromosome. Functional analysis of the HMW adhesins produced by three unrelated strains demonstrated that each isolate possesses one protein with HMW1-like adherence properties and another with HMW2-like adherence properties. These findings suggest that the hmw1 and hmw2 loci may have arisen via a gene duplication event in an ancestral strain. In addition, they support the hypothesis that the distinct binding specificities of HMW1 and HMW2 emerged early and have persisted over time, suggesting an ongoing selective advantage.
authors.affiliation: Edward Mallinckrodt Department of Pediatrics, Washington University Medical School, St. Louis, Missouri 63110, USA.
authors.forename: Amy Z
authors.initials: AZ
authors.lastname: Buscher
authors.forename: Katie
authors.initials: K
authors.lastname: Burmeister
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Barenkamp
authors.forename: Joseph W
authors.initials: JW
authors.lastname: St  Geme
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000502:physiology*; D001422:Bacterial Adhesion; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008970:Molecular Weight; D010802:Phylogeny; D020161:Physical Chromosome Mapping
chemical_list: D018829:Adhesins, Bacterial