Comprehensive two-dimensional supercritical fluid and gas chromatography with independent fast programmed heating of the gas chromatographic column.
With comprehensive two-dimensional supercritical fluid and fast, independent temperature-programmed gas chromatography (SFCxGC), a polar column was used in the first dimension to achieve group-type analysis. The eluent of this separation was repetitively sampled and transferred to a fast, resistively heated gas chromatograph to obtain the boiling point distribution over the entire polarity separation. The SFC was operated isothermally with stopped flow to provide a sufficient time span for the GC analysis. The GC analysis had a typical cycle time of 1 min for the system demonstrated here. During this time, the GC column was independently heated at a rate of 450 degrees C/min to 250 degrees C and actively cooled again to -50 degrees C before the next GC injection took place. The analysis of petrochemical samples is presented to illustrate the technique.
Venter
Andre
A
Chemistry Department, University of Pretoria, South Africa.
Rohwer
Egmont R
ER
D002849:Chromatography, Gas / Q000295:instrumentation* / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation* / Q000379:methods; D006358:Hot Temperature; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013696:Temperature