Investigations of oocyte in vitro maturation within a mouse model.
This study attempted to develop a 'less meiotically competent' murine model for oocyte in vitro maturation (IVM), which could more readily be extrapolated to human clinical assisted reproduction. Oocyte meiotic competence was drastically reduced upon shortening the standard duration of in vivo gonadotrophin stimulation from 48 h to 24 h, and by selecting only naked or partially naked germinal vesicle oocytes, instead of fully cumulus enclosed oocyte complexes. With such a less meiotically competent model, only porcine granulosa coculture significantly enhanced the oocyte maturation rate in vitro, whereas no significant enhancement was observed with macaque and murine granulosa coculture. Increased serum concentrations and the supplementation of gonadotrophins, follicular fluid and extracellular matrix gel within the culture medium did not enhance IVM under either cell-free or coculture conditions. Culture medium conditioned by porcine granulosa also enhanced the maturation rate, and this beneficial effect was not diminished upon freeze-thawing. Enhanced IVM in the presence of porcine granulosa coculture did not, however, translate into improved developmental competence, as assessed by in vitro fertilization and embryo culture to the blastocyst stage.
Chin
Alexis Heng Boon
AH
Department of Obstetrics & Gynaecology, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074. bc_heng@hotmail.com
Chye
Ng Soon
NS
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008954:Models, Biological; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D013552:Swine