Induction of c-fos expression in the mouse brain associated with hyperalgesia induced by intrathecal injection of protein kinase C activator.
Here, we found that a single intrathecal (i.t.) administration of a protein kinase C (PKC) activator, phorbol 12,13-dibutyrate (PDBu), induced pain-like behaviors in mice. Furthermore, i.t.-administered PDBu caused the increased c-fos-like immunoreactivity in the parafascicular nuclei (PF), amygdala and cingulate cortex (CG), but not hippocampus. These findings suggest that the stimulation of spinal PKC results in an enhancement of neuronal activity in the PF, amygdala and CG associated with hyperalgesia.
Narita
Minoru
M
Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan. narita@hoshi.ac.jp
Imai
Satoshi
S
Oe
Kousei
K
Narita
Michiko
M
Kubota
Chiharu
C
Yajima
Yoshinori
Y
Yamazaki
Mitsuaki
M
Suzuki
Tsutomu
T
D000679:Amygdala / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators; D006179:Gyrus Cinguli / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D007278:Injections, Spinal; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology
D020536:Enzyme Activators; D016760:Proto-Oncogene Proteins c-fos; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C