title: Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes.
abstract: Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, ORFeome clones representing 1,282 Arabidopsis TF genes have been obtained in the Gateway high throughput cloning pENTR vector, including 411 genes whose annotation lack cDNA support. All the ORFeome inserts have also been mobilized into a yeast expression destination vector, with an estimated 85% rate of expressing the respective proteins. Sequence analysis of these clones revealed that 34 of them did not match with either the reported cDNAs or current predicted open-reading-frame sequences. Among those, novel alternative splicing of TF gene transcripts is responsible for the observed differences in at least five genes. However, those alternative splicing events do not appear to be differentially regulated among distinct Arabidopsis tissues examined. Lastly, expression of those TF genes in 17 distinct Arabidopsis organ types and the cultured cells was profiled using a 70-mer oligo microarray.
authors.affiliation: Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, and the National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Shen
authors.forename: Li-Geng
authors.initials: LG
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Du
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Wang
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Yang
authors.forename: Li-De
authors.initials: LD
authors.lastname: Hu
authors.forename: Xin-Fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Danmeng
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Tan
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Mu
authors.forename: Da-Bing
authors.initials: DB
authors.lastname: Zhang
authors.forename: Yu-Le
authors.initials: YL
authors.lastname: Liu
authors.forename: S P
authors.initials: SP
authors.lastname: Dinesh-Kumar
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Xi-Ping
authors.initials: XP
authors.lastname: Wang
authors.forename: Hong-Ya
authors.initials: HY
authors.lastname: Gu
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Qu
authors.forename: Shu-Nong
authors.initials: SN
authors.lastname: Bai
authors.forename: Ying-Tang
authors.initials: YT
authors.lastname: Lu
authors.forename: Jia-Yang
authors.initials: JY
authors.lastname: Li
authors.forename: Jin-Dong
authors.initials: JD
authors.lastname: Zhao
authors.forename: Jianru
authors.initials: J
authors.lastname: Zuo
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Xing Wang
authors.initials: XW
authors.lastname: Deng
authors.forename: Yu-Xian
authors.initials: YX
authors.lastname: Zhu
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D014157:Transcription Factors