Plexin-B3 is a functional receptor for semaphorin 5A.
Semaphorins are a large family of molecular cues implicated in neural development and in a variety of functions outside the nervous system. Semaphorin 5A (Sema5A) is a transmembrane semaphorin, containing seven thrombospondin type-1 repeats, which was recently found to control axon guidance. Here we show that plexin-B3 is a high-affinity receptor specific for Sema5A. We further demonstrate that plexin-B3 activation by Sema5A mediates functional responses in plexin-B3-expressing cells (either fibroblasts, epithelial and primary endothelial cells). In addition, Sema5A can trigger the intracellular signalling of the hepatocyte growth factor/scatter factor receptor, Met, associated in a complex with plexin-B3. We thus conclude that Sema5A is able to elicit multiple functional responses through its receptor plexin-B3.
Artigiani
Stefania
S
Institute for Cancer Research and Treatment (IRCC), University of Torino--Medical School, 10060 Candiolo (TO), Italy.
Conrotto
Paolo
P
Fazzari
Pietro
P
Gilestro
Giorgio F
GF
Barberis
Davide
D
Giordano
Silvia
S
Comoglio
Paolo M
PM
Tamagnone
Luca
L
D000818:Animals; D001369:Axons / Q000378:metabolism; D015153:Blotting, Western; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism* / Q000502:physiology; D011485:Protein Binding; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D039961:Semaphorins; D015398:Signal Transduction
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; C496426:PLXNB3 protein, human; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; C491228:SEMA5A protein, human; D039961:Semaphorins; D019859:Proto-Oncogene Proteins c-met