An Arabidopsis homolog of the bacterial cell division inhibitor SulA is involved in plastid division.
Plastids have evolved from an endosymbiosis between a cyanobacterial symbiont and a eukaryotic host cell. Their division is mediated both by proteins of the host cell and conserved bacterial division proteins. Here, we identified a new component of the plastid division machinery, Arabidopsis thaliana SulA. Disruption of its cyanobacterial homolog (SSulA) in Synechocystis and overexpression of an AtSulA-green fluorescent protein fusion in Arabidopsis demonstrate that these genes are involved in cell and plastid division, respectively. Overexpression of AtSulA inhibits plastid division in planta but rescues plastid division defects caused by overexpression of AtFtsZ1-1 and AtFtsZ2-1, demonstrating that its role in plastid division may involve an interaction with AtFtsZ1-1 and AtFtsZ2-1.
Raynaud
Cécile
C
Institut de Biotechnologie des Plantes, Equipe Cycle Cellulaire Unité Mixte de Recherche 8618 Centre National de la Recherche Scientifique, Orsay Cedex, France. raynaud@ibp.u-psud.fr
Cassier-Chauvat
Corinne
C
Perennes
Claudette
C
Bergounioux
Catherine
C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D001419:Bacteria / Q000166:cytology*; D002455:Cell Division; D018506:Gene Expression Regulation, Plant; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010641:Phenotype; D018087:Plastids / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
D029681:Arabidopsis Proteins; C436953:AtFtsZ2 protein, Arabidopsis; D011993:Recombinant Fusion Proteins; C492756:SulA protein, Arabidopsis