Efficacy of a recombinant chimeric anti-hCG antibody to prevent human cytotrophoblasts fusion and block progesterone synthesis.
OBJECTIVE
A recombinant chimeric antibody against hCG (cPIPP) has been engineered and expressed at high yield in plants. The purpose of this work was to enquire whether this antibody is competent to neutralize the bioactivity of hCG on human trophoblasts.


METHODS
Cytotrophoblast cells, isolated from term placentae were maintained in culture for 3 days in presence or absence of humanized chimeric anti-hCG antibodies. Progesterone secreted was quantitated by ELISA. Fusion and cyto-architecture of the cells was studied by light and electron microscopy. Modulation of E-cadherin was investigated using RT-PCR and immunocytochemistry.


RESULTS
Recombinant chimeric anti-hCG antibody blocked the synthesis of progesterone by trophoblasts. No fusion of cytotrophoblasts to form syncytium took place. E-cadherin, a vital cell adhesion molecule involved in cell-to-cell interaction did not show differentiation related decline in its expression in presence of the antibody.


CONCLUSIONS
Recombinant chimeric anti-hCG antibody (cPIPP) was effective to neutralize hCG induced bioactivities in the human derived trophoblast cells.
Dhar
Ruby
R
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Karmakar
Subhradip
S
Sriraman
Rajan
R
Talwar
Gursaran Prasad
GP
Das
Chandana
C
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology*; D015820:Cadherins / Q000032:analysis / Q000235:genetics; D002449:Cell Aggregation / Q000502:physiology; D002459:Cell Fusion; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D010920:Placenta / Q000166:cytology; D011374:Progesterone / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015820:Cadherins; D006063:Chorionic Gonadotropin; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D011374:Progesterone