Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors.
The basis for differences in activity-dependent trafficking of AMPA receptors (AMPARs) and NMDA receptors (NMDARs) remains unclear. Using single-molecule tracking, we found different lateral mobilities for AMPARs and NMDARs: changes in neuronal activity modified AMPAR but not NMDAR mobility, whereas protein kinase C activation modified both. Differences in mobility were mainly detected for extrasynaptic AMPARs, suggesting that receptor diffusion between synaptic and extrasynaptic domains is involved in plasticity processes.
Groc
Laurent
L
UMR 5091 CNRS-Université Bordeaux 2, Bordeaux 33077, France.
Heine
Martin
M
Cognet
Laurent
L
Brickley
Kieran
K
Stephenson
F Anne
FA
Lounis
Brahim
B
Choquet
Daniel
D
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D002232:Carbocyanines / Q000493:pharmacokinetics; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013569:Synapses / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D000906:Antibodies; D002232:Carbocyanines; C409466:NR1 NMDA receptor; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C089187:cyanine dye 3; D013779:Tetrodotoxin; D011189:Potassium Chloride; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; C104722:glutamate receptor ionotropic, AMPA 2