title: Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii.
abstract: The salt-dependent stability of recombinant dimeric isocitrate dehydrogenase [ICDH; isocitrate: NADP oxidoreductase (decarboxylating), EC 1.1.1.42] from the halophilic archaeon Haloferax volcanii (Hv) was investigated in various conditions. Hv ICDH dissociation/deactivation was measured to probe the respective effect of anions and cations on stability. Surprisingly, enzyme stability was found to be mainly sensitive to cations and very little (or not) sensitive to anions. Divalent cations induced a strong shift of the active/inactive transition towards low salt concentration. A high resistance of Hv ICDH to chemical denaturation was also found. The data were analysed and are discussed in the framework of the solvation stability model for halophilic proteins.
authors.affiliation: Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale CEA-CNRS-UJF, 41 Rue Jules Horowitz, 38042, Grenoble, France. madern@ibs.fr
authors.forename: Dominique
authors.initials: D
authors.lastname: Madern
authors.forename: Mónica
authors.initials: M
authors.lastname: Camacho
authors.forename: Adoración
authors.initials: A
authors.lastname: Rodríguez-Arnedo
authors.forename: María-José
authors.initials: MJ
authors.lastname: Bonete
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Zaccai
mesh_terms: D000838:Anions / Q000737:chemistry; D019540:Area Under Curve; D001665:Binding Sites; D002412:Cations; D002942:Circular Dichroism; D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D004795:Enzyme Stability; D019622:Haloferax volcanii / Q000201:enzymology*; D007477:Ions; D007521:Isocitrate Dehydrogenase / Q000737:chemistry*; D008956:Models, Chemical; D008962:Models, Theoretical; D009249:NADP / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D012492:Salts / Q000737:chemistry / Q000494:pharmacology*; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D000838:Anions; D002412:Cations; D007477:Ions; D012492:Salts; D012997:Solvents; D012965:Sodium Chloride; D009249:NADP; D007521:Isocitrate Dehydrogenase