title: Olfactory horizontal basal cells demonstrate a conserved multipotent progenitor phenotype.
abstract: Stem cells of adult regenerative organs share a common goal but few established conserved mechanisms. Within the neural stem cell niche of the mouse olfactory epithelium, we identified a combination of extracellular matrix (ECM) receptors that regulate adhesion and mitosis in non-neural stem cells [intercellular adhesion molecule-1 (ICAM-1), beta1, beta4, and alpha-1, -3, and -6 integrins] and on horizontal basal cells (HBCs), candidate olfactory neuro-epithelial progenitors. Using ECM receptors as our guide, we recreated a defined microenvironment in vitro that mimics olfactory basal lamina and, when supplemented with epidermal growth factor, transforming growth factor alpha, and leukemia inhibitory factor, allows us to preferentially expand multiple clonal adherent colony phenotypes from individual ICAM-1+ and ICAM-1+/beta1 integrin+-selected HBCs. The most highly mitotic colony-forming HBCs demonstrate multipotency, spontaneously generating more ICAM-positive presumptive HBCs, a combination of olfactory neuroglial progenitors, and neurons of olfactory and potentially nonolfactory phenotypes. HBCs thus possess a conserved adhesion receptor expression profile similar to non-neural stem cells, preferential self-replication in an in vitro environment mimicking their in vivo niche, and contain subpopulations of cells that can produce multiple differentiated neuronal and glial progeny from within and beyond the olfactory system in vitro.
authors.affiliation: Department of Zoology and Center for Molecular Medicine and Therapeutics, University of British Columbia, V6T 1Z4 Vancouver, Canada.
authors.forename: Lindsay A
authors.initials: LA
authors.lastname: Carter
authors.forename: Jessica L
authors.initials: JL
authors.lastname: MacDonald
authors.forename: A Jane
authors.initials: AJ
authors.lastname: Roskams
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005109:Extracellular Matrix / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D016023:Integrins / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D051379:Mice; D008938:Mitosis; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009831:Olfactory Mucosa / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype
chemical_list: D016023:Integrins; D018799:Intercellular Adhesion Molecule-1