Evaluation of the mass spectrometric fragmentation of codeine and morphine after 13C-isotope biosynthetic labeling.
All major fragment ions of codeine and morphine were elucidated using LC-electrospray MS/MS and high resolution FT-ICR-MS combined with an IRMPD system. Nanogram quantities of labeled codeine were isolated and purified from Papaver somniferum seedlings, which were grown for up to 9 days in the presence of [ring-13C6]-l-tyrosine, [ring-13C6]-tyramine and [1,2-13C2], [6-O-methyl 13C]-(R,S)-coclaurine. The labeling degree of codeine up to 57% into morphinans was observed.
Poeaknapo
Chotima
C
Biozentrum Universität Halle, Weinbergweg 22, D-06120 Halle/S., Germany.
Fisinger
Ursula
U
Zenk
Meinhart H
MH
Schmidt
Jürgen
J
D002247:Carbon Isotopes; D003061:Codeine / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007553:Isotope Labeling / Q000379:methods; D009020:Morphine / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D002247:Carbon Isotopes; D009020:Morphine; D003061:Codeine