Purification and characterization of recombinant Staphylococcus haemolyticus DNA gyrase and topoisomerase IV expressed in Escherichia coli.
The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating type II topoisomerases of a coagulase-negative staphylococcal species.
Bronstein
Joel C
JC
Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. Joel.Bronstein@pfizer.com
Olson
Stacey L
SL
LeVier
Kristin
K
Tomilo
Mark
M
Weber
Peter C
PC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D027101:DNA Topoisomerase IV / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D041162:Staphylococcus haemolyticus / Q000187:drug effects / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase