title: Expression of retinoic acid-inducible gene-I in vascular smooth muscle cells stimulated with interferon-gamma.
abstract: Vascular smooth muscle cells (SMC) play an important role in atherogenesis and vasospasm. Interferon-gamma (IFN-gamma) is a potent cytokine that regulates immune and inflammatory responses by inducing multiple genes in many types of cells including SMC. Retinoic acid-inducible gene-I (RIG-I) is a putative RNA helicase, but its physiological function is not known. RIG-I is induced in leukemic cells by retinoic acid or in endothelial cells by lipopolysaccharide. We have studied the expression of RIG-I in cultured SMC from human umbilical artery. IFN-gamma stimulated SMC to express RIG-I mRNA and protein in concentration- and time-dependent manners. Immunohistochemical analysis revealed the expression of RIG-I in SMC in vivo. We conclude that RIG-I may play some pathophysiological role in immune and inflammatory reactions in SMC.
authors.affiliation: Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. timaizum@cc.hirosaki-u.ac.jp
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Norito
authors.initials: N
authors.lastname: Yagihashi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Koji
authors.initials: K
authors.lastname: Yamashita
authors.forename: Akira
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Kageaki
authors.initials: K
authors.lastname: Taima
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Ituro
authors.initials: I
authors.lastname: Inoue
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Soroku
authors.initials: S
authors.lastname: Yagihashi
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases