title: [Influence of FasL overexpression in transgenic mice on the immune regulative function of Sertoli cell].
abstract: OBJECTIVE
  To study the influence of FasL overexpression in transgenic mice on the Sertoli cell immune response to testicular infection.
  
  
  METHODS
  Ureaplasma urealyticum (UU) was directly injected into bladders of FasL transgenic mice and wild-type mice respectively, to mimic an ascending infection pathway. At week 1, 2 and 3 after injection, respectively, the mice were put to death to observe the pathological alterations in testis section. And at the same time the differences of FasL, TGF-beta, IL-1alpha and IL-6 expressions on Sertoli cells were compared by immunohistochemical staining between wild mice and transgenic mice before and after infection, respectively. The high-purified Sertoli cells were isolated from the testis tissue of wild-type mice, comparing apoptotic capability of Fas(+) Jurkat cells mediated by FasL(+) Sertoli cells of wild control and wild UU-infected groups.
  
  
  RESULTS
  The pathological changes of testis tissue from transgenic mice were more serious as compared with wild-type mice and the model of cytokines secreted by sertoli cells was distinctive between the two kinds of mice. The UU-infected Sertoli cells increased Fas(+) Jurkat cell apoptosis.
  
  
  CONCLUSIONS
  FasL overexpression can influence the cytokine's secretion in the process of anti-infection immunity and further affects the immune balance of testis locality. Not all FasL over expression is benefit to body's anti-infection immune response.
authors.affiliation: Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Zhao-ping
authors.initials: ZP
authors.lastname: Liu
authors.forename: Wei-yi
authors.initials: WY
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Ma
authors.forename: Jia-hua
authors.initials: JH
authors.lastname: Hu
authors.forename: Guang-jie
authors.initials: GJ
authors.lastname: Chen
authors.forename: Ye-bin
authors.initials: YB
authors.lastname: Xi
authors.forename: Bao-guo
authors.initials: BG
authors.lastname: Wang
authors.forename: Shi-san
authors.initials: SS
authors.lastname: Bao
authors.forename: Li-he
authors.initials: LH
authors.lastname: Guo
mesh_terms: D000818:Animals; D017209:Apoptosis; D016207:Cytokines / Q000378:metabolism*; D053222:Fas Ligand Protein; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D019169:Jurkat Cells / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D012708:Sertoli Cells / Q000378:metabolism*; D013733:Testicular Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D016869:Ureaplasma Infections / Q000378:metabolism / Q000473:pathology*; D016990:Ureaplasma urealyticum
chemical_list: D016207:Cytokines; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007375:Interleukin-1; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D016212:Transforming Growth Factor beta