title: Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent.
abstract: We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83 microM of phyA.
authors.affiliation: Department of Biomedical Engineering, 136 Hudson Hall, Duke University, Durham, North Carolina 27708, USA. chyang@caltech.edu
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
authors.forename: Michael A
authors.initials: MA
authors.lastname: Choma
authors.forename: Laura E
authors.initials: LE
authors.lastname: Lamb
authors.forename: John D
authors.initials: JD
authors.lastname: Simon
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Izatt
mesh_terms: D003287:Contrast Media / Q000032:analysis / Q000737:chemistry*; D005217:Fat Emulsions, Intravenous / Q000032:analysis* / Q000737:chemistry; D005240:Feasibility Studies; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D010834:Phytochrome / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D051942:Phytochrome A; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003287:Contrast Media; D005217:Fat Emulsions, Intravenous; D015335:Molecular Probes; D051942:Phytochrome A; D010834:Phytochrome