title: Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI.
abstract: After aneuploidy, sperm premature chromatin condensation (PCC) is the next prevalent cause of fertilization failure. Therefore the aim of this study was to evaluate the effect of sperm protamine deficiency on sperm PCC formation post-ICSI. Chromatin analysis was carried out on failed fertilized oocytes post-ICSI and incidences of sperm PCC were evaluated and the results were compared with the extent of protamine deficiency assessed by chromomycin A3. The results show that incidence of sperm PCC was significantly different in failed fertilized oocytes injected from semen samples with greater or less than 30% CMA3 positivity (P = 0.04). However, except for fertilization rate (P < 0.001), the mean number of MII, MI and germinal vesicles oocytes and percentage normal sperm were not significantly different between the two groups (P > 0.05). A significant correlation was observed between sperm protamine deficiency with fertilization rate. Hence sperm protamine deficiency affects fertilization rate and possibly prones sperm to PCC post-ICSI.
authors.affiliation: Royan Institute, Tehran, Iran. mh_nasr@med.mui.ac.ir
authors.forename: M H
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: S
authors.initials: S
authors.lastname: Razavi
authors.forename: H
authors.initials: H
authors.lastname: Mozdarani
authors.forename: M
authors.initials: M
authors.lastname: Mardani
authors.forename: H
authors.initials: H
authors.lastname: Azvagi
mesh_terms: D000328:Adult; D002843:Chromatin / Q000502:physiology*; D002877:Chromosomes, Human / Q000502:physiology*; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D008938:Mitosis / Q000502:physiology*; D009865:Oocytes / Q000502:physiology; D011479:Protamines / Q000378:metabolism*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*; D017211:Treatment Failure
chemical_list: D002843:Chromatin; D011479:Protamines