Regulation of dendritic protein synthesis by miniature synaptic events.
We examined dendritic protein synthesis after a prolonged blockade of action potentials alone and after a blockade of both action potentials and miniature excitatory synaptic events (minis). Relative to controls, dendrites exposed to a prolonged blockade of action potentials showed diminished protein synthesis. Dendrites in which both action potentials and minis were blocked showed enhanced protein synthesis, suggesting that minis inhibit dendritic translation. When minis were acutely blocked or stimulated, an immediate increase or decrease, respectively, in dendritic translation was observed. Taken together, these results reveal a role for miniature synaptic events in the acute regulation of dendritic protein synthesis in neurons.
Sutton
Michael A
MA
Division of Biology, Howard Hughes Medical Institute (HHMI), California Institute of Technology, Pasadena, CA 91125, USA.
Wall
Nicholas R
NR
Aakalu
Girish N
GN
Schuman
Erin M
EM
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D017930:Genes, Reporter; D006624:Hippocampus / Q000166:cytology; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D015398:Signal Transduction; D013111:Spider Venoms / Q000494:pharmacology; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects; D013572:Synaptic Vesicles / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D016194:Receptors, N-Methyl-D-Aspartate; D013111:Spider Venoms; D013779:Tetrodotoxin; C020763:alpha-latrotoxin; D019274:Botulinum Toxins, Type A