Phospholipase C-beta 2 as a mammalian taste signaling marker is expressed in the multiple gustatory tissues of medaka fish, Oryzias latipes.
Phospholipase C-beta 2 (PLC-beta 2) is a key enzyme in mammalian taste signal transduction. To analyze the taste system in fishes at molecular level, we cloned mfplc-beta 2 as a medaka fish homologue of PLC-beta 2. In situ hybridization analysis revealed that mfplc-beta 2 is expressed in the lip and branchial region where chemosensory tissues are distributed. Immunohistochemical detection of nerve fibers near the mfplc-beta 2 positive cells suggests the characteristic of peripheral sensory cells. These results suggest that mfplc-beta 2 is expressed in the gustatory sensory cells of medaka. This may provide a molecular basis for the taste reception at multiple tissues in fish species.
Yasuoka
Akihito
A
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Aihara
Yoshiko
Y
Matsumoto
Ichiro
I
Abe
Keiko
K
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D001934:Branchial Region / Q000378:metabolism; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D054799:Phospholipase C beta; D010802:Phylogeny; D015347:RNA Probes; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013649:Taste / Q000235:genetics / Q000502:physiology*; D010738:Type C Phospholipases / Q000235:genetics* / Q000378:metabolism
D015415:Biomarkers; D007527:Isoenzymes; D015347:RNA Probes; D010738:Type C Phospholipases; D054799:Phospholipase C beta