title: Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry.
abstract: A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the diode array detector and atmospheric pressure chemical ionization (APCI) mass spectrometer. More than 52 components in the methanol extract of R. chuanxiong were resolved and 11 of them were preliminary identified according to their UV and mass spectra.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 161, Zhongshan Road, Dalian 116011, China.
authors.forename: Xueguo
authors.initials: X
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
authors.forename: Xingye
authors.initials: X
authors.lastname: Su
authors.forename: Hongjing
authors.initials: H
authors.lastname: Fu
authors.forename: Jianyi
authors.initials: J
authors.lastname: Ni
authors.forename: Ruihuan
authors.initials: R
authors.lastname: Zhao
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal