Isoforms of cyclic AMP response element binding proteins in Drosophila S2 cells.
Activation or inhibition of the cyclic AMP (cAMP)-protein kinase A (PKA) pathway can ultimately regulate the transcription of a variety of genes. In vertebrates, the best characterized nuclear targets of PKA are the 'cAMP response element' (CRE) binding proteins (CREB). Differences in the transcriptional response to this pathway between cells and tissues can be based on the presence of distinct CREB isoforms. In this context, we have now investigated the presence of different dCREB transcripts in a stable, embryonic insect cell line, i.e., Drosophila Schneider 2 (S2) cells. In addition, we have studied the possible effect of cellular cAMP- and Ca2+ increases on the expression of a luciferase reporter in cells transfected with a CRE-containing reporter gene construct. In combination with recent data from the literature, our results indicate that the regulation of CRE-dependent gene expression shows some important differences between insects and vertebrates.
Poels
Jeroen
J
Laboratory for Developmental Physiology, Genomics and Proteomics, Naamsestraat 59, B-3000 Leuven, Belgium.
Franssens
Vanessa
V
Van Loy
Tom
T
Martinez
Alberto
A
Suner
Marie-Marthe
MM
Dunbar
Stuart J
SJ
De Loof
Arnold
A
Vanden Broeck
Jozef
J
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins; D002460:Cell Line; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D004330:Drosophila / Q000166:cytology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D017931:DNA Primers; D018076:DNA, Complementary