title: Oncogenic role of eIF-5A2 in the development of ovarian cancer.
abstract: Amplification of 3q26 is one of the most frequent chromosomal alterations in many solid tumors, including ovarian, lung, esophageal, prostate, breast, and nasopharyngeal cancers. A candidate oncogene to eukaryotic initiation factor 5A2 (eIF-5A2), a member of eukaryotic initiation factor 5A subfamily, has been isolated from a frequently amplified region at 3q26.2. In this work, the tumorigenic ability of eIF-5A2 was demonstrated by anchorage-independent growth in soft agar and tumor formation in nude mice. Furthermore, antisense DNA against eIF-5A2 could inhibit cell growth in ovarian cancer cell line UACC-1598 with amplification of eIF-5A2 in form of double minutes. Cell growth rate in UACC-1598 was also inhibited when the expression level of EIF-5A2 was decreased by the reduction of the copy number of double minutes. The correlation of EIF-5A2 overexpression and clinical features of ovarian cancer was investigated using tissue microarray, and the result showed that eIF-5A2 overexpression was significantly associated with the advanced stage of ovarian cancer. These findings suggest that eIF-5A2 plays important roles in ovarian pathogenesis.
authors.affiliation: Department of Clinical Oncology, The University of Hong Kong, People's Republic of China. xyguan@hkucc.hku.hk
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Guan
authors.forename: Jackie M-W
authors.initials: JM
authors.lastname: Fung
authors.forename: Ning-Fang
authors.initials: NF
authors.lastname: Ma
authors.forename: Sze-Hang
authors.initials: SH
authors.lastname: Lau
authors.forename: Lai-Shan
authors.initials: LS
authors.lastname: Tai
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Hu
authors.forename: Qiu-Liang
authors.initials: QL
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Jonathan S T
authors.initials: JS
authors.lastname: Sham
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D016373:DNA, Antisense / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010448:Peptide Initiation Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D016373:DNA, Antisense; D010448:Peptide Initiation Factors; C425967:eIF-5A2