Analysis of the cytogenetic stability of the human embryonal kidney cell line 293 by cytogenetic and STR profiling approaches.
We have characterized the cytogenetic alterations of the human embryonal cell line 293 by spectral karyotyping and G-banding analysis. To investigate its genomic stability, we compared the karyotypes of 293 and its daughter line EcR-293. Genotype profiling through short tandem repeats complemented the analysis. While displaying almost identical STR profiles and thus verifying their origin and their close relation, the two lines were remarkably different in their number of chromosomes and setup of aberrant chromosomes. However, the cell lines retained a stable karyotype in long term culture. The establishment of subclones from EcR-293, expressing inducible lacZ or MEN1 transgenes, only added minor changes to the karyotype. Our study shows that the cytogenetic constitution of a clonal cell line of the 293 origin appears to be sufficiently stable. However, care should be taken when comparing the properties of independent 293 lineages, since clonal variations might be substantial.
Bylund
L
L
Department of Molecular Medicine, Karolinska Institutet, Stockholm, Sweden. lovisa.bylund@cmm.ki.se
Kytölä
S
S
Lui
W-O
WO
Larsson
C
C
Weber
G
G
D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D006801:Humans; D007621:Karyotyping; D007668:Kidney / Q000196:embryology; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D020080:Tandem Repeat Sequences; D013997:Time Factors; D019076:Transgenes
C105373:MEN1 protein, human; D011518:Proto-Oncogene Proteins