Biochemical characteristics of a mutant of the methanoarchaeon Methanothermobacter thermautotrophicus resistant to the protonophoric uncoupler TCS.
In an attempt to more closely define a protein basis of differences in ATPase and ATP synthase activities in a mutant of the methanoarchaeon Methanothermobacter thermautotrophicus resistant to the protonophoric uncoupler TCS (3,3',4',5-tetrachlorosalicylanilide), the composition of membrane associated proteins from the wild-type and mutant strains has been compared. The uncoupler-resistance in the mutant strain was not accompanied by changes in a protein size or changes in the level of subunits A, B and c (proteolipid) of the A1A0-type ATPase-synthase. On the other hand, we revealed a 670-kDa membrane-associated protein complex that is abundantly present only in the mutant strain; it is composed of at least 5 different subunits of 95, 52, 42, 29 and 22 kDa.
Cijbonová
L
L
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28 Ivanka pri Dunaji, Slovakia.
Majerník
A
A
Smigán
P
P
D019843:Archaeal Proteins / Q000032:analysis / Q000302:isolation & purification; D015153:Blotting, Western; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis / Q000302:isolation & purification; D017013:Methanobacteriaceae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D009154:Mutation; D021122:Protein Subunits / Q000032:analysis / Q000302:isolation & purification; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000302:isolation & purification; D012458:Salicylanilides / Q000494:pharmacology*
D019843:Archaeal Proteins; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D021122:Protein Subunits; D012458:Salicylanilides; D006180:Proton-Translocating ATPases; C003390:3,3',4',5-tetrachlorosalicylanilide