Isolation and partial structural evaluation of a cardiotoxic factor from Indian common murrel (Channa striatus L.) skin extract.
It was earlier reported from this laboratory that, Channa striatus, L a common edible fish, whose skin extract (CSSE) was pharmacologicaclly potent and contains several bioactive compounds. In the present communication a cardiotoxic factor was isolated and purified by thin layer chromatography followed by silica gel and neutral alumina column chromatography. Spectroscopic studies (UV, IR, 1H and 13C NMR, FAB-MS) indicated that the lethal cardiotoxic factor (CSS-CTF II) was an aromatic alkaloid compound with -NH, > C = C < and -OH functional groups. The molecular weight was found to be 413 dalton. LD50 of CSS-CTF II was found to be 42.5 mg/kg (i.v) in Swiss albino male mice. Pharmacological studies showed that CSS-CTF II possesses hypotensive and cardiotoxic activities and produced death through apnoea in experimental animals but had no effect on nerve muscle preparations. The haematological and biochemical data also indicated the toxic nature of CSS-CTF II, through significant fall in haemoglobin, total RBC, WBC, platelet count and increased cardiac marker enzyme CPK and CPK-MB value in experimental animals. The present investigation thus established the toxic nature of CSS-CTF II isolated from edible fish C. striatus skin extract. Further work is needed to identify CSS-CTF II's mechanism of action and its antagonism for therapeutic purpose.
Karmakar
S
S
Laboratory of Toxicology and Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, India.
Das
Tulika
T
Ghosh
A
A
Dasgupta
S C
SC
Biswas
A K
AK
Gomes
A
A
D000818:Animals; D001794:Blood Pressure; D002339:Carotid Arteries / Q000187:drug effects; D002415:Cats; D002855:Chromatography, Thin Layer; D005397:Fish Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005399:Fishes; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects; D012867:Skin / Q000378:metabolism*; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013997:Time Factors; D014466:Ultraviolet Rays
C488386:CSS-CTF II, Channa striatus; D005397:Fish Venoms