MMP inhibition prevents human lens epithelial cell migration and contraction of the lens capsule.
OBJECTIVE
The development of posterior capsule contraction following cataract surgery is caused by the activity of residual lens epithelial cells. Matrix metalloproteinases (MMPs) are a group of proteolytic enzymes, which are essential for cell migration and cell mediated contraction following wound healing. The authors investigated whether inhibiting MMP activity can reduce lens epithelial cell migration and as a result, lead to a reduction in cell mediated capsule contraction.


METHODS
Human donor lens capsules were cultured and treated with a broad spectrum MMP inhibitor, Ilomastat (GM6001). MMP-2 and MMP-9 production were determined by ELISA. Cell migration onto the posterior capsule and capsule contraction were digitally measured.


RESULTS
MMP inhibition significantly reduced lens epithelial cell migration onto the posterior capsule (p<0.05), and a reduction in capsule contraction was observed (p<0.05).


CONCLUSIONS
Ilomastat significantly reduced lens epithelial cell migration onto the posterior capsule surface and inhibited capsule contraction. MMP inhibition may have a role in the therapeutic treatment of posterior capsule opacification.
Wong
T T L
TT
Department of Pathology, Institute of Ophthalmology, 11-43 Bath Street, London EC1V 9EL, UK. tina.wong@ucl.ac.uk
Daniels
J T
JT
Crowston
J G
JG
Khaw
P T
PT
D002387:Cataract Extraction / Q000009:adverse effects*; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006877:Hydroxamic Acids; D007211:Indoles / Q000494:pharmacology; D007903:Lens Capsule, Crystalline / Q000166:cytology / Q000187:drug effects; D007908:Lens, Crystalline / Q000166:cytology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D061965:Matrix Metalloproteinase Inhibitors
D006877:Hydroxamic Acids; D007211:Indoles; D061965:Matrix Metalloproteinase Inhibitors; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C119203:ilomastat