Oncogenic role of eIF-5A2 in the development of ovarian cancer.
Amplification of 3q26 is one of the most frequent chromosomal alterations in many solid tumors, including ovarian, lung, esophageal, prostate, breast, and nasopharyngeal cancers. A candidate oncogene to eukaryotic initiation factor 5A2 (eIF-5A2), a member of eukaryotic initiation factor 5A subfamily, has been isolated from a frequently amplified region at 3q26.2. In this work, the tumorigenic ability of eIF-5A2 was demonstrated by anchorage-independent growth in soft agar and tumor formation in nude mice. Furthermore, antisense DNA against eIF-5A2 could inhibit cell growth in ovarian cancer cell line UACC-1598 with amplification of eIF-5A2 in form of double minutes. Cell growth rate in UACC-1598 was also inhibited when the expression level of EIF-5A2 was decreased by the reduction of the copy number of double minutes. The correlation of EIF-5A2 overexpression and clinical features of ovarian cancer was investigated using tissue microarray, and the result showed that eIF-5A2 overexpression was significantly associated with the advanced stage of ovarian cancer. These findings suggest that eIF-5A2 plays important roles in ovarian pathogenesis.
Guan
Xin-Yuan
XY
Department of Clinical Oncology, The University of Hong Kong, People's Republic of China. xyguan@hkucc.hku.hk
Fung
Jackie M-W
JM
Ma
Ning-Fang
NF
Lau
Sze-Hang
SH
Tai
Lai-Shan
LS
Xie
Dan
D
Zhang
Yu
Y
Hu
Liang
L
Wu
Qiu-Liang
QL
Fang
Yan
Y
Sham
Jonathan S T
JS
D000818:Animals; D002455:Cell Division / Q000235:genetics; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D016373:DNA, Antisense / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010448:Peptide Initiation Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
D016373:DNA, Antisense; D010448:Peptide Initiation Factors; C425967:eIF-5A2