title: Influence of ethylene oxide sterilization on the activity of native reindeer bone morphogenetic protein.
abstract: We studied the effects of ethylene oxide sterilization (Steri-Vac 4XL, temperature 29 degrees C, exposure time 4 h 10 min, ethylene oxide concentration 860 mg/l) on the osteoinductivity of partially purified native reindeer bone morphogenetic protein (BMP) in a hind leg muscle pouch model of male NMRI mice. BMP was administered in implants containing 3 mg in a collagen carrier. Implants without sterilization and without BMP served as controls. New bone formation was evaluated based on the calcium yield, radiographic and histological examination 3 weeks after implantation. The implants without BMP were not able to induce new bone visible in radiographs. In the sterilized BMP group, the mean area of new bone was 35% ( p=0.004) and density 32% ( p=0.000) smaller than in the nonsterilized group. Calcium yield was 20% lower in the sterilized group than in the nonsterilized group, but this difference was not significant ( p=0.22). It was many times lower in the group without BMP than in the above-mentioned groups ( p=0,001). We conclude that ethylene oxide gas sterilization reduces the bone-forming activity of native reindeer BMP by one third.
authors.affiliation: Bone Transplantation Research Group, University of Oulu, Aapistie 5, PO Box 5000, 90014 Oulu, Finland. Tarmo.Pekkarinen@uta.fi
authors.forename: T
authors.initials: T
authors.lastname: Pekkarinen
authors.forename: O
authors.initials: O
authors.lastname: Hietala
authors.forename: T S
authors.initials: TS
authors.lastname: Lindholm
authors.forename: P
authors.initials: P
authors.lastname: Jalovaara
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000737:chemistry; D019485:Bone Morphogenetic Proteins; D005027:Ethylene Oxide; D008297:Male; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D012053:Reindeer; D018709:Statistics, Nonparametric; D013242:Sterilization / Q000379:methods*
chemical_list: D019485:Bone Morphogenetic Proteins; D005027:Ethylene Oxide