Capillary zone electrophoresis as a tool to monitor the last stages of the degradation of water-sensitive polymers.
In order to monitor the formation of the water-soluble by-products from chain-scission of degradable polymers used in the biomedical field, four capillary electrophoresis methods are discussed with the aim of giving the limits and performance for each. Three of them (electroosmotic flow reversal by dynamic adsorption of a polycation, multilayer polyelectrolyte coatings and physical binding of polyethylene oxide) are based on the use of dynamic coatings onto the inner surface of a fused silica capillary, a simple means to adapt performance to specific separations via modification and control of the electroosmotic flow of fused capillary. Using oligomers of lactic acid considered as standards the methods are compared. Other examples of ester-containing macromolecules (poly(hydroxybutyrate)), as well as degradable polyanions are described, namely N-acetylneuraminate polymer and poly(beta-malic acid).
Braud
Christian
C
CRBA, CNRS, UMR 5473, Faculté de Pharmacie, Université Montpellier 1, 15 avenue Charles Flahault, BP 14491, 34093 Montpellier 5, France. braud@univ-montp1.fr
D001673:Biodegradation, Environmental; D004337:Drug Carriers / Q000032:analysis / Q000378:metabolism*; D016903:Drug Monitoring / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006885:Hydroxybutyrates / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism; D011108:Polymers / Q000032:analysis / Q000378:metabolism* / Q000627:therapeutic use; D012794:Sialic Acids / Q000378:metabolism; D014867:Water; D000094122:Polyhydroxybutyrates
D004337:Drug Carriers; D006885:Hydroxybutyrates; D019344:Lactic Acid; D008293:Malates; D011091:Polyesters; D011108:Polymers; D012794:Sialic Acids; D014867:Water; C121060:poly(malic acid); C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C033616:poly(lactide)