IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.
In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.
Fukutomi
Yasuo
Y
Leprosy Research Center, National institute of Infectious Diseases, Tokyo, Japan. fukutomi@nih.go.jp
Matsuoka
Masanori
M
Minagawa
Fumishige
F
Toratani
Satoshi
S
McCormick
Gregory
G
Krahenbuhl
James
J
D000818:Animals; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D003470:Culture Media; D016753:Interleukin-10 / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000382:microbiology*; D051379:Mice; D009166:Mycobacterium leprae / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature
D003470:Culture Media; D016753:Interleukin-10