title: Rat strains differ in susceptibility to maternal and fetal infection with Mycoplasma pulmonis.
abstract: OBJECTIVE
  Vaginally infected Sprague-Dawley (SD) rats are more susceptible to adverse pregnancy outcomes than Wistar (WIS) rats. We postulated that SD rats have enhanced hematogenous spread of Mycoplasma pulmonis to fetal tissues.
  
  
  METHODS
  WIS and SD dams were infected intravenously with 10(7), 10(6), and 10(5) colony-forming units of M. pulmonis at gestation day 14. Dams and six randomly selected fetuses were cultured at days 15, 16, 17, and 18 of gestation.
  
  
  RESULTS
  In the high-dose group, 100% of fetuses were colonized regardless of rat strain. Significantly higher numbers of M. pulmonis were isolated from placenta (low dose, P < 0.0001; medium dose, P < 0.024; high dose, P < 0.0001), amniotic fluid (low dose, P < 0.003; medium dose, P < 0.017), and fetuses (low dose, P < 0.0011) of SD rats. Spread of M. pulmonis to the amniotic fluid and fetus occurred 1 day earlier in SD rats.
  
  
  CONCLUSIONS
  The difference in susceptibility between the two rat strains cannot be explained by hematogenous spread alone. The relative resistance to adverse pregnancy outcomes in WIS rats may be a function of a more robust innate immune system. These rat strains may represent an animal model to address host resistance factors to intrauterine infection.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611-0880, USA. lreyes@vpha.health.ufl.edu
authors.forename: Leticia
authors.initials: L
authors.lastname: Reyes
authors.forename: Micheal
authors.initials: M
authors.lastname: Shelton
authors.forename: Margaret
authors.initials: M
authors.lastname: Riggs
authors.forename: Mary B
authors.initials: MB
authors.lastname: Brown
mesh_terms: D000653:Amniotic Fluid / Q000382:microbiology; D000818:Animals; D015169:Colony Count, Microbial; D004198:Disease Susceptibility; D005260:Female; D005333:Fetus / Q000382:microbiology*; D009175:Mycoplasma Infections / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D045803:Mycoplasma pulmonis / Q000166:cytology / Q000502:physiology*; D010920:Placenta / Q000382:microbiology / Q000473:pathology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013154:Spleen / Q000382:microbiology; D013997:Time Factors