Influence of molecule CD 11b blockade on the course of acute ceruleine pancreatitis in rats.
Polymorphonuclear cells (PMN) activation is an essential step in acute pancreatitis (AP). We investigated the activation status of PMN, oxidative stress and pancreatic damage in early stage of experimental ceruleine pancreatitis in rats. The PMN action was modulated by monoclonal antibody CD 11b administration. The circulating WBC and polymorphonuclear cells count was reduced after AP induction. Chemiluminescence of whole blood PMN was remarkably reduced in AP group and increased after MoAb CD 11b administration. The CD 11b blockade significantly reduced the WBC infiltration and malondialdehyde (MDA) concentration within pancreatic gland. These data suggest that activated PMN are an important factor in early AP pathogenesis. Neutrophil aggregation within pancreatic gland modulated by monoclonal antibody CD11b contribute to the extent of injury during the early stage of ceruleine experimental pancreatitis in rats.
Hać
Stanisław
S
Department of General Gastroenterological and Endocrine Surgery, Medical University of Gdañsk, Gdansk, Poland. sthac@amg.gda.pl
Dobosz
Marek
M
Kaczor
Jacek
J
Rzepko
Robert
R
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000935:Antifungal Agents / Q000633:toxicity*; D039481:CD11b Antigen / Q000276:immunology*; D002569:Cerulenin / Q000633:toxicity*; D004195:Disease Models, Animal; D019272:Leukocyte Elastase / Q000097:blood; D008163:Luminescent Measurements; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000935:Antifungal Agents; D039481:CD11b Antigen; D002569:Cerulenin; D008315:Malondialdehyde; D009195:Peroxidase; D019272:Leukocyte Elastase