title: Design and synthesis of a solid-supported FR225659 derivative for its receptor screening.
abstract: [structure: see text] We describe the design and synthesis of latex particles attached to an FR225659 derivative to identify its receptor proteins. Two key building blocks were prepared by two-step degradation of FR225659 under basic conditions. The designed ligand showed an acceptable level of biological activity to make it of potential value for use in affinity-supported receptor identification. Affinity purification of FR225659-binding proteins using the latex nanoparticles provided three candidate receptor peptides for the biological activity.
authors.affiliation: Department of Applied Chemistry, Tokyo Institute of Technology, Meguro, Tokyo 152-8552, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Zenkoh
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hatori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Setoi
authors.forename: Haruma
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
authors.forename: Takashi
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D002384:Catalysis; D007202:Indicators and Reagents; D020128:Inhibitory Concentration 50; D007840:Latex / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C478488:(4-hydroxy-3-(2-(2-propenyl)-4-quinoline)benzoyl)-(3-chloro-4-hydroxyarginyl)-(3-hydroxy-3-methylprolyl)-dehydrovaline; D002352:Carrier Proteins; D007202:Indicators and Reagents; D007840:Latex; D009842:Oligopeptides