Novel protein-protein interactions of the Yersinia pestis type III secretion system elucidated with a matrix analysis by surface plasmon resonance and mass spectrometry.
Binary complexes formed by components of the Yersinia pestis type III secretion system were investigated by surface plasmon resonance (SPR) and matrix-assisted laser desorption time-of-flight mass spectrometry. Pairwise interactions between 15 recombinant Yersinia outer proteins (Yops), regulators, and chaperones were first identified by SPR. Mass spectrometry confirmed over 80% of the protein-protein interactions suggested by SPR, and new binding partners were further characterized. The Yop secretion protein (Ysc) M2 of Yersinia enterocolitica and LcrQ of Y. pestis, formerly described as ligands only for the specific Yop chaperone (Syc) H, formed stable complexes with SycE. Additional previously unreported complexes of YscE with the translocation regulator protein TyeA and the thermal regulator protein YmoA and multiple potential protein contacts by YscE, YopK, YopH, and LcrH were also identified. Because only stably folded proteins were examined, the interactions we identified are likely to occur either before or after transfer through the injectosome to mammalian host cells and may have relevance to understanding disease processes initiated by the plague bacterium.
Swietnicki
Wieslaw
W
United States Army Medical Research Institute of Infectious Diseases, NCI, National Institutes of Health, Frederick, Maryland 21702, USA.
O'Brien
Sarah
S
Holman
Kari
K
Cherry
Scott
S
Brueggemann
Ernst
E
Tropea
Joseph E
JE
Hines
Harry B
HB
Waugh
David S
DS
Ulrich
Robert G
RG
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D018832:Molecular Chaperones / Q000737:chemistry; D016366:Open Reading Frames; D011485:Protein Binding; D017510:Protein Folding; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors; D015534:Trans-Activators / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry; D015007:Yersinia / Q000378:metabolism; D015010:Yersinia pestis / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008024:Ligands; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C118339:SycD protein, bacteria; C081269:SycE protein, Yersinia; D015534:Trans-Activators; D014157:Transcription Factors; C079210:YmoA protein, Yersinia enterocolitica; C110345:YscM2 protein, Yersinia enterocolitica; C074427:lcrQ protein, Yersinia pseudotuberculosis; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia