A novel KRAB zinc-finger protein, ZNF480, expresses in human heart and activates transcriptional activities of AP-1 and SRE.
The zinc-finger motif found in many transcription factors is thought to be important for human heart development and diseases. In this study, we have identified and characterized a novel zinc-finger gene named ZNF480 using degenerate primers from an early human embryo heart cDNA library. ZNF480 contains a KRAB-A box and 12 C2H2 zinc fingers. The cDNA sequence contains an open reading frame of 1551 bp, encoding a putative protein of 516 amino acid residues with a predicted molecular mass of 57 kDa. Northern blot analysis indicates that a 4.7kb transcript specific for ZNF480 is expressed only in embryonic heart. In the adult tissues, the expression of ZNF480 is restricted largely to heart, skeletal muscle, pancreas, and placenta. Overexpression of ZNF480 in cells activates the transcriptional activities of AP-1 and SRE. Therefore, our data suggest that ZNF480 may act as a positive regulator in MAPK-mediated signaling pathways that lead to the activation of AP-1 and SRE.
Yi
Zhengfang
Z
The Center for Heart Development, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, PR China.
Li
Yongqing
Y
Ma
Wenbin
W
Li
Dali
D
Zhu
Chuanbing
C
Luo
Jian
J
Wang
Yuequn
Y
Huang
Xinqiong
X
Yuan
Wuzhou
W
Liu
Mingyao
M
Wu
Xiushan
X
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D018808:Transcription Factor AP-1 / Q000502:physiology*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C488406:ZNF480 protein, human