Bioengineered teeth from cultured rat tooth bud cells.
The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
Duailibi
M T
MT
University Federal of São Paulo, Department of Otorhinolaryngology and Human Communication Disorders, Sao Paolo, Brazil.
Duailibi
S E
SE
Young
C S
CS
Bartlett
J D
JD
Vacanti
J P
JP
Yelick
P C
PC
D020341:Absorbable Implants; D000367:Age Factors; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D019344:Lactic Acid / Q000737:chemistry; D008567:Membranes, Artificial; D009805:Odontogenesis / Q000502:physiology*; D009852:Omentum / Q000601:surgery; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation; D014083:Tooth Germ / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid