title: Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.
abstract: The vertebrate nuclear pore complex (NPC) is a macromolecular assembly of protein subcomplexes forming a structure of eightfold radial symmetry. The NPC core consists of globular subunits sandwiched between two coaxial ring-like structures of which the ring facing the nuclear interior is capped by a fibrous structure called the nuclear basket. By postembedding immunoelectron microscopy, we have mapped the positions of several human NPC proteins relative to the NPC core and its associated basket, including Nup93, Nup96, Nup98, Nup107, Nup153, Nup205, and the coiled coil-dominated 267-kDa protein Tpr. To further assess their contributions to NPC and basket architecture, the genes encoding Nup93, Nup96, Nup107, and Nup205 were posttranscriptionally silenced by RNA interference (RNAi) in HeLa cells, complementing recent RNAi experiments on Nup153 and Tpr. We show that Nup96 and Nup107 are core elements of the NPC proper that are essential for NPC assembly and docking of Nup153 and Tpr to the NPC. Nup93 and Nup205 are other NPC core elements that are important for long-term maintenance of NPCs but initially dispensable for the anchoring of Nup153 and Tpr. Immunogold-labeling for Nup98 also results in preferential labeling of NPC core regions, whereas Nup153 is shown to bind via its amino-terminal domain to the nuclear coaxial ring linking the NPC core structures and Tpr. The position of Tpr in turn is shown to coincide with that of the nuclear basket, with different Tpr protein domains corresponding to distinct basket segments. We propose a model in which Tpr constitutes the central architectural element that forms the scaffold of the nuclear basket.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, S-17177 Stockholm, Sweden.
authors.forename: Sandra
authors.initials: S
authors.lastname: Krull
authors.forename: Johan
authors.initials: J
authors.lastname: Thyberg
authors.forename: Birgitta
authors.initials: B
authors.lastname: Björkroth
authors.forename: Hans-Richard
authors.initials: HR
authors.lastname: Rackwitz
authors.forename: Volker C
authors.initials: VC
authors.lastname: Cordes
mesh_terms: D006367:HeLa Cells; D006801:Humans; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D046912:Multiprotein Complexes; D022022:Nuclear Pore / Q000378:metabolism* / Q000648:ultrastructure; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D034622:RNA Interference
chemical_list: D046912:Multiprotein Complexes; C079377:NUP153 protein, human; C440251:NUP160 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C073597:TPR protein, human