Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: characterization of heat shock-induced promoters.
The appropriate conditions to switch on the heat shock promoters in Corynebacterium glutamicum were defined by Northern blot analysis. Transcriptional patterns were characterized for the groEL2 gene and the groES-groEL1 and dnaK operons. Transcriptional start points of these genes were determined by primer extension analysis, allowing the identification of CIRCE and HAIR boxes close to the -10 and -35 regions of the promoters. The presence of both CIRCE and HAIR sequences within a single promoter (P-groEL2) in bacteria is described for the first time. In addition, the dnaK promoter showed -10 and -35 sequences similar to those recognized by SigH of Mycobacterium and SigR of Streptomyces close to a second transcription start region with -10 and -35 boxes typical of promoters for housekeeping genes.
Barreiro
Carlos
C
Institute of Biotechnology of León (INBIOTEC). Science Park of León. Av. Real, 1, León, 24006, Spain.
González-Lavado
Eva
E
Pátek
Miroslav
M
Martín
Juan-Francisco
JF
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics; D018835:Chaperonin 10 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D017124:Conserved Sequence; D003352:Corynebacterium / Q000235:genetics* / Q000502:physiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics; D009876:Operon; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics; D013302:Streptomyces / Q000235:genetics; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C120328:SigH protein, bacteria; D012808:Sigma Factor