Proteomic profiling of proteins decreased in hepatocellular carcinoma from patients infected with hepatitis C virus.
Hepatocellular carcinoma (HCC) is a major cause of death in Japan. It has been suggested that hepatitis C virus (HCV) plays an important role in hepatocarcinogenesis, because of high incidence among the patients. To understand the mechanism of hepatocarcinogenesis after HCV infection, we performed a comparative study on the protein profiles between tumorous and nontumorous specimens from the patients infected with HCV by means of two-dimensional electrophoresis. Eleven spots were decreased in HCC tissues from over 50% of the patients. Eight proteins out of 11 spots were identified using peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. These proteins were liver type aldolase, tropomyosin beta-chain, ketohexokinase, enoyl-CoA hydratase, albumin, smoothelin, ferritin light chain, and arginase 1. The intensity of enoyl-CoA hydratase, tropomyosin beta-chain, ketohexokinase, liver type aldolase, and arginase 1 was significantly different (p < 0.05). The decrease of 8 proteins was characteristic in HCC. We will discuss the implication of these proteins for the loss of function of hepatocytes and for the possibility of carcinogenesis of HCV-related HCC.
Yokoyama
Yuichiro
Y
Department of Gastroenterology and Hepatology, Yamaguchi University School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8605, Japan.
Kuramitsu
Yasuhiro
Y
Takashima
Motonari
M
Iizuka
Norio
N
Toda
Toshifusa
T
Terai
Shuji
S
Sakaida
Isao
I
Oka
Masaaki
M
Nakamura
Kazuyuki
K
Okita
Kiwamu
K
D001119:Arginase / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005631:Fructokinases / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000821:virology*; D010455:Peptides / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014335:Tropomyosin / Q000378:metabolism
D010455:Peptides; D020543:Proteome; D014335:Tropomyosin; D005631:Fructokinases; C021475:ketohexokinase; D001119:Arginase; D005634:Fructose-Bisphosphate Aldolase; D004746:Enoyl-CoA Hydratase