Candida albicans CTN gene family is induced during macrophage infection: homology, disruption and phenotypic analysis of CTN3 gene.
We have isolated a Candida albicans gene, coding for a putative peroxisomal carnitine acetyl transferase (CTN) protein, which is up-regulated during macrophage infection. In the present study, we describe the disruption of CTN3 gene (previously called CAT3) to gain insight into its potential role during infection. The ability of disrupted Candida mutants to filament was affected by several solid media. Northern blot analysis revealed that CTN3 gene may be involved not only in conditions of cell starvation but also during the process of germination. In agreement with the putative peroxisomal localization of the corresponding protein, we observed a strong glucose repression of CTN3 gene and, on the contrary, high level of transcription by carbon sources that induce the formation of peroxisomal proteins. Furthermore, we showed the existence of two additional C. albicans CTN encoding sequences, which are also induced during macrophage infection.
Prigneau
Odile
O
Laboratory of Molecular Fungal Pathogenesis, Institute of Genetics and Biophysics Buzzati Traverso, Via Marconi, 12-80125 Naples, Italy.
Porta
Amalia
A
Maresca
Bruno
B
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002176:Candida albicans / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D002332:Carnitine O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D002460:Cell Line; D019295:Computational Biology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D010641:Phenotype; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D002332:Carnitine O-Acetyltransferase; D005947:Glucose