Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form.
A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could determine nanomolar concentrations of ATP in its fixed form via interactions with biotin-conjugated antibodies.
Nakamura
Makiko
M
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Mie
Masayasu
M
Funabashi
Hisakage
H
Kobatake
Eiry
E
D000255:Adenosine Triphosphate / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D000255:Adenosine Triphosphate; D019809:Streptavidin; D008156:Luciferases