pH-dependent conformational changes of ferricytochrome c induced by electrode surface microstructure.
pH-dependent processes of bovine heart ferricytochrome c have been investigated by electronic absorption and circular dichroism (CD) spectra at functionalized single-wall carbon nanotubes (SWNTs) modified glass carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. These methods enabled the pH-dependent conformational changes arising from the heme structure change to be monitored. The spectra obtained at functionalized SWNTs/GCE reflect electrode surface microstructure-dependent changes for pH-induced protein conformation, pK(a) of alkaline transition and structural microenvironment of the ferricytochrome c heme. pH-dependent conformational distribution curves of ferricytochrome c obtained by analysis of in situ CD spectra using singular value decomposition least square (SVDLS) method show that the functionalized SWNTs can retain native conformational stability of ferricytochrome c during alkaline transition.
Jiang
Xiue
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Qu
Xiaohu
X
Zhang
Lei
L
Zhang
Zheling
Z
Jiang
Junguang
J
Wang
Erkang
E
Dong
Shaojun
S
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013057:Spectrum Analysis
D045304:Cytochromes c; D007501:Iron