Potassium hexacyanoferrate(III)-mediated fluorometric determination of melatonin.
This paper describes the development of a novel, simple and sensitive high-performance liquid chromatographic method for the determination of melatonin using precolumn fluorometric derivatization. A strong fluorescence was induced when alkaline solution of melatonin was heated in the presence of potassium hexacyanoferrate (III). This fluorescent reaction was used for the precolumn derivatization of melatonin. Solid phase extraction was used for concentrating melatonin below a level of detection limit.
Iizuka
Hideaki
H
Faculty of Pharmaceutical Sciences, Toho University 2-2-1 Miyama Funabashi, Chiba 274-8510, Japan. iizuka@phar.toho-u.ac.jp
Yajima
Takehiko
T
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005292:Ferricyanides; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007211:Indoles; D008550:Melatonin / Q000032:analysis* / Q000592:standards; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D005292:Ferricyanides; D007202:Indicators and Reagents; D007211:Indoles; D008550:Melatonin; C028033:potassium ferricyanide; C032159:acetonitrile