Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries.
In a preliminary study aimed at developing strategies for the simultaneous detection of various biologically important molecules, a procedure is described that allows the electrochemical detection of nitric oxide (NO) released by a population of human umbilical vein endothelial cells (HUVEC) by using an array of electrodes comprising three individually addressable electrodes. Each electrode in the array was modified with a different NO-sensitive electrocatalyst, thereby demonstrating the possibility of modifying the individual electrodes in an array with different sensing chemistries. This study opens a doorway to the development of arrays of electrodes for the simultaneous detection of multiple analytes in a complex environment by suitably tailoring the sensitivity and selectivity of each electrode in the array to a specific analyte in the test medium.
Oni
Joshua
J
Anal. Chem.-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr 150, 44780 Bochum, Germany.
Pailleret
Alain
A
Isik
Sonnur
S
Diab
Nizam
N
Radtke
Ina
I
Blöchl
Andrea
A
Jackson
Michael
M
Bedioui
Fethi
F
Schuhmann
Wolfgang
W
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004563:Electrochemistry; D004566:Electrodes; D042783:Endothelial Cells / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D013499:Surface Properties
D009569:Nitric Oxide; D010984:Platinum