Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction.
A method is described for enabling safe transit of denatured virus samples for polymerase chain reaction (PCR) identification without the risk of unwanted viable viruses. Cotton swabs dipped in avian infectious bronchitis virus (IBV) or avian pneumovirus (APV) were allowed to dry. Newcastle disease virus and avian influenza viruses were used as controls. Autoclaving and microwave treatment for as little as 20 sec destroyed the infectivity of all four viruses. However, both IBV and APV could be detected by reverse transcriptase (RT)-PCR after autoclaving and as long as 5 min microwave treatment (Newcastle disease virus and avian influenza viruses were not tested). Double microwave treatment of IBV and APV with an interval of 2 to 7 days between was tested. After the second treatment, RT-PCR products were readily detected in all samples. Swabs from the tracheas and cloacas of chicks infected with IBV shown to contain infectious virus were microwaved. Swabs from both sources were positive by RT-PCR. Microwave treatment appears to be a satisfactory method of inactivating virus while preserving nucleic acid for PCR identification.
Elhafi
G
G
Department of Veterinary Pathology, University of Liverpool, Leahurst, South Wirral, UK.
Naylor
C J
CJ
Savage
C E
CE
Jones
R C
RC
D000818:Animals; D002645:Chickens / Q000821:virology*; D006358:Hot Temperature; D001351:Infectious bronchitis virus / Q000235:genetics / Q000472:pathogenicity* / Q000528:radiation effects; D029121:Metapneumovirus / Q000235:genetics / Q000472:pathogenicity* / Q000528:radiation effects; D008872:Microwaves; D020133:Reverse Transcriptase Polymerase Chain Reaction; D038301:Virus Inactivation / Q000528:radiation effects*