[The effect of ligustrazine on the expression of CD44 in bone marrow of bone marrow transplantation mice].
The aim of this study was to investigate the effects of ligustrazine on the expression of CD44 in bone marrow tissue at the early phase of hematopoietic reconstruction after bone marrow transplantation (BMT) in mice. The mice were divided into 3 groups: normal mice without BMT treatment, BMT only and BMT added with ligustrazine groups. On the days 7, 14, 21, 28 after BMT, peripheral blood cells and bone marrow nucleated cells (BMNC) were counted, while the expression of CD44 was assayed by flow cytometry. CFU-S was determined on the day 10 after BMT. The results showed that in ligustrazine group the WBC, Plt and BMNC counts and hematopoietic tissue area estimation in bone marrow on the days 7, 14, 21, 28 after BMT were all higher than in BMT controlled group, CFU-S counts were higher than in BMT only group too. On the contrary, mature RBC volume was lower than in the BMT only group. The expression of CD44 in ligustrazine group on days 7, 14, 21, 28 after BMT was significantly higher than in BMT only group. It is concluded that ligustrazine can enhance CD44 expression in bone marrow of syngenic BMT mice, which may be one of mechanisms of ligustrazine accelerating hematopoietic reconstitution in bone marrow.
Luo
Lin
L
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Wen-Li
WL
Sun
Han-Ying
HY
Zhou
Jian-Feng
JF
Fu
Li
L
Liu
Dan
D
Xu
Hui-Zhen
HZ
Lu
Wu
W
D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000737:chemistry* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000401:mortality; D005260:Female; D006410:Hematopoiesis; D018960:Hyaluronan Receptors / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000494:pharmacology*
D018960:Hyaluronan Receptors; D011719:Pyrazines; C017953:tetramethylpyrazine