title: In vitro effects of the polyphenols resveratrol, mangiferin and (-)-epigallocatechin-3-gallate on the scuticociliate fish pathogen Philasterides dicentrarchi.
abstract: This study investigated the in vitro effects of the polyphenols resveratrol, mangiferin and (-)-epigallocatechin-3-gallate (EGCG) on the histiophagous ciliate Philasterides dicentrarchi, which causes fatal scuticociliatosis in farmed turbot Scophthalmus maximus L. Of the 3 polyphenols, resveratrol showed strongest antiprotozoal activity, reducing ciliate density after 1 wk culture by, on average, 91% at 50 microM, and 96% at 500 microM. EGCG reduced ciliate density by, on average, 93% at 500 microM, with no significant effect at 50 microM. Mangiferin reduced ciliate density by, on average, 56% at 500 microM, again with no significant effect at 50 microM. In view of these findings, we discuss the potential utility of chemotherapy with polyphenols as a strategy for the control of scuticociliatosis in farmed turbot.
authors.affiliation: Laboratorio de Parasitología, Instituto de Investigación y Análisis Alimentarios, Universidad de Santiago de Compostela, Constantino Candeira 15782, Santiago de Compostela, Spain. mpleiro@usc.es
authors.forename: J
authors.initials: J
authors.lastname: Leiro
authors.forename: J A
authors.initials: JA
authors.lastname: Arranz
authors.forename: A
authors.initials: A
authors.lastname: Paramá
authors.forename: M F
authors.initials: MF
authors.lastname: Alvarez
authors.forename: M L
authors.initials: ML
authors.lastname: Sanmartín
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000469:parasitology*; D005412:Flatfishes; D066298:In Vitro Techniques; D016802:Oligohymenophorea / Q000187:drug effects*; D011529:Protozoan Infections, Animal / Q000517:prevention & control*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000981:Antiprotozoal Agents; D013267:Stilbenes; D044004:Xanthones; C013592:mangiferin; D002392:Catechin; C045651:epigallocatechin gallate; D000077185:Resveratrol