On the separation, detection and quantification of pectin derived oligosaccharides by capillary electrophoresis.
Having previously reported that capillary electrophoresis can be used as a tool for the analysis of partially methyl-esterified oligogalacturonides we now describe a method that improves the resolution of individual oligomers, and detail a more rigorous quantification scheme that uses an internal standard and takes into account the relative molecular absorbance of different partially methyl-esterified species. The internal consistency of the method is subsequently demonstrated by performing the quantification of an endo-polygalacturonase pectin digest before and after de-methylation of the resultant oligomers.
Ström
Anna
A
Unilever R&D Colworth, Sharnbrook, MK44 1LQ Bedford, UK.
Williams
Martin A K
MA
D004586:Electrophoresis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006603:Hexuronic Acids / Q000737:chemistry; D007477:Ions; D008745:Methylation; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D010368:Pectins / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D013379:Substrate Specificity; D013997:Time Factors; D014466:Ultraviolet Rays
D006603:Hexuronic Acids; D007477:Ions; D009844:Oligosaccharides; D011134:Polysaccharides; C007819:galacturonic acid; D010368:Pectins; D011096:Polygalacturonase