Development of an automated sample preparation module for environmental monitoring of biowarfare agents.
An automated sample preparation module, based upon sequential injection analysis (SIA), has been developed for use within an autonomous pathogen detection system. The SIA system interfaced aerosol sampling with multiplexed microsphere immunoassay-flow cytometric detection. Metering and sequestering of microspheres using SIA was found to be reproducible and reliable, over 24-h periods of autonomous operation. Four inbuilt immunoassay controls showed excellent immunoassay and system stability over five days of unattended continuous operation. Titration curves for two biological warfare agents, Bacillus anthracis and Yersinia pestis, obtained using the automated SIA procedure were shown to be similar to those generated using a manual microtiter plate procedure.
Hindson
Benjamin J
BJ
Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94551, USA.
Brown
Steve B
SB
Marshall
Graham D
GD
McBride
Mary T
MT
Makarewicz
Anthony J
AJ
Gutierrez
Dora M
DM
Wolcott
Duane K
DK
Metz
Thomas R
TR
Madabhushi
Ramakrishna S
RS
Dzenitis
John M
JM
Colston
Billy W
BW
D000907:Antibodies, Bacterial / Q000276:immunology; D001408:Bacillus anthracis / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D001694:Biological Warfare; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D013997:Time Factors; D015010:Yersinia pestis / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity
D000907:Antibodies, Bacterial