title: The role of the DRY motif of human MC4R for receptor activation.
abstract: We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Tottori, Japan. yyamano@muses.tottori-u.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamano
authors.forename: Rio
authors.initials: R
authors.lastname: Kamon
authors.forename: Takao
authors.initials: T
authors.lastname: Yoshimizu
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Toda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Oshida
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Chaki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Isao
authors.initials: I
authors.lastname: Morishima
mesh_terms: D020816:Amino Acid Motifs / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D000242:Cyclic AMP / Q000096:biosynthesis; D006801:Humans; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D011485:Protein Binding; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014162:Transfection
chemical_list: D008024:Ligands; C470072:MC4R protein, human; D044105:Receptor, Melanocortin, Type 4; D043562:Receptors, G-Protein-Coupled; D000242:Cyclic AMP