title: Effect of KLP-602 on virus replication in cell cultures.
abstract: The effect of KLP-602 (active substance: lysozyme dimer) on the replication of two animal viruses: the TK900 strain of Aujeszky's disease virus and the Roakin strain of the Newcastle disease virus were investigated. The maximal tolerable dose of the drug was determined for two cell cultures (CECC and GMK) and the effect of the medicine on the titre range of infectious viruses and their adsorption was assayed. The direct impact of KLP-602 on the viral strains used was also determined. And finally the replication dynamics of viruses in the presence of KLP-602 preparation was estimated. KLP-602 showed no direct effect on either the viruses applied in the study or their adsorption. The drug, introduced into the culture 24 hours before its infection, did not affect the replication of the pseudorabies virus, but decreased the titre of the Newcastle disease virus. KLP-602 introduced simultaneously with the infection considerably lowered the final titres of both viruses. The medicine had the greatest inhibitory effect on the replication dynamics of both types of viruses in the CECC and of the pseudorabies virus in the GMK culture upon the maximal tolerable concentrations of drug and low infectious doses of viruses applied.
authors.affiliation: Department of Infectious and Invasive Diseases, Chair of Clinical Microbiology and Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn Oczapowskiego 13, 10-718 Olsztyn, Poland. stenia@uwm.edu.pl
authors.forename: J
authors.initials: J
authors.lastname: Małaczewska
authors.forename: Z
authors.initials: Z
authors.lastname: Rotkiewicz
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line / Q000821:virology; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D011558:Herpesvirus 1, Suid / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009113:Muramidase / Q000008:administration & dosage / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; C488571:KLP602; D009113:Muramidase