title: Cell-adhesive and blood-coagulant properties of ultrathin poly(methyl methacrylate) stereocomplex films.
abstract: Isotactic (it) and syndiotactic (st) poly(methyl methacrylate)s (PMMAs) were assembled on a poly(ethylene terephthalate) cell disk (as a solid substrate), which is normally used for cell culture, to produce ultrathin stereocomplex films. Static contact angles of the films using air bubbles in aqueous phase revealed the stepwise assembly of both polymers. More L929 fibroblast cells adhered and were flattened on the ultrathin stereocomplex films, compared to homogeneous PMMA films and to spin-coated films comprised of a mixed solution of it- and st-PMMAs with a molar ratio of 1 : 2, which is the stoichiometry of a stereocomplex. The difference in the number of adherent cells was greatest after the first 3 h of incubation. Pre-adsorption of proteins, which are related to cell adhesion, onto the stereocomplex film potentially modulated the cell-adhesive properties. Fresh whole human blood did not coagulate on the complex film for 20 min, although blood coagulated after 15 min on the homogeneous films and on the 1 : 2 mixed film. Platelets did not adhere onto the complex films after 15 min of incubation, which was consistent with the results of blood coagulation. These observations indicate that the stereocomplex formation of PMMAs on the surface of films strongly affects the bioactivity of these films.
authors.affiliation: Department of Nanostructured and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Serizawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamashita
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D010973:Platelet Adhesiveness; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D000267:Tissue Adhesions
chemical_list: D001672:Biocompatible Materials; D011506:Proteins; D019904:Polymethyl Methacrylate