title: Intrinsic factor-mediated modulation of cyanocobalamin-N-sulfonyl-acridinium-9-carboxamide chemiluminescence.
abstract: Two cyanocobalamin-N-sulfonyl-acridinium-9-carboxamides with linkage through the N(10) or 9-position were prepared from B(12)-e-carboxylic acid. The noncovalent association of intrinsic factor with these ligands resulted in specific modulation of the associated chemiluminescence signal either by quenching or changing the emission profile. Either effect was sufficient to formulate a homogeneous assay to detect vitamin B(12) in buffer.
authors.affiliation: Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
authors.forename: Maciej
authors.initials: M
authors.lastname: Adamczyk
authors.forename: Donald D
authors.initials: DD
authors.lastname: Johnson
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Mattingly
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Moore
authors.forename: You
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000166:Acridines / Q000737:chemistry / Q000494:pharmacology; D007437:Intrinsic Factor / Q000187:drug effects*; D049449:Luminescence; D008958:Models, Molecular; D008968:Molecular Conformation; D013329:Structure-Activity Relationship; D014805:Vitamin B 12 / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000166:Acridines; D007437:Intrinsic Factor; D014805:Vitamin B 12