A rapid and sensitive method for the detection of Xanthomonas fragariae, causal agent of angular leafspot disease in strawberry plants.
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming, too insensitive or impractical when handling a large number of samples routinely. Here we describe a modified protocol of the REDExtract-N-Amp Plant PCR-Kit for the detection of X. fragariae in planta and demonstrate that it provides greater sensitivity, speed and high throughput potential than methods previously described.
Stöger
Anna
A
Austrian Agency for Health and Food Safety, Competence Center of Biochemistry, Spargelfeldstrasse 191, A-1226 Vienna, Austria.
Ruppitsch
Werner
W
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D031985:Fragaria / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014974:Xanthomonas / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*
D004269:DNA, Bacterial