(Z)-3,5,4'-Tri-O-methyl-resveratrol, induces apoptosis in human lymphoblastoid cells independently of their p53 status.
The pro-apoptotic ability of (Z)-3,5,4'-Tri-O-methyl-resveratrol (R3) was investigated in vitro on the human lymphoblastoid cell line TK6 and its p53-knockout counterpart (NH32). In both cell lines, R3 induced the stimulation of caspase-3. Although R3 induced growth inhibition and apoptosis of both cell lines, two distinct mechanisms were observed. The p53-knockout NH32 cells were shown to override the G2/M phase checkpoint with development of hyperdiploid cells, whereas TK6 cells accumulated at G2/M. As p53 function is often altered in human cancer cells, these results show that the pro-apototic effects of R3 against tumor cells are independent of their p53 status.
Schneider
Yann
Y
Laboratory of Nutritional Oncology, Inserm UMR S392, IRCAD, 1 place de l'Hôpital, BP 426, 67091 Strasbourg Cedex, France.
Fischer
Barbara
B
Coelho
David
D
Roussi
Stamatiki
S
Gosse
Francine
F
Bischoff
Pierre
P
Raul
Francis
F
D000840:Anisoles / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000494:pharmacology; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D016158:Genes, p53 / Q000235:genetics*; D005818:Genetic Engineering; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology; D013267:Stilbenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology
C497286:3,4',5-trimethoxystilbene; D000840:Anisoles; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases