Flow cytometric screening of aldolase catalytic antibodies.
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
Shim
Hyunbo
H
The Skaggs Institute for Chemical Biology and Departments of Molecular Biology and Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Karlström
Amelie
A
Touami
Sofia M
SM
Fuller
Roberta P
RP
Barbas
Carlos F
CF
D000818:Animals; D017151:Antibodies, Catalytic / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D005634:Fructose-Bisphosphate Aldolase / Q000187:drug effects / Q000378:metabolism*; D006825:Hybridomas; D007700:Kinetics; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D014162:Transfection
D017151:Antibodies, Catalytic; D011994:Recombinant Proteins; D005634:Fructose-Bisphosphate Aldolase