[Culture of mice marrow stromal stem cells in human acellular cartilage matrix].
OBJECTIVE
To study the feasibility of using mice marrow stromal stem cells(MSCs) as seed cells for tissue engineering cartilage to embed the seed cells in acellular cartilage matrix of human auricle.


METHODS
Acellular cartilage matrix was made from human auricle cartilage. The MSCs were isolated from the nucleated cells fraction of mice marrow by centrifuge. The MSCs were embedded in acellular cartilage matrix. After 10 day's combined culture, the specimens were observed with optical and electrical microscope.


RESULTS
The MSCs could well proliferate in the acellular cartilage matrix. The cells were not well-distributed in acellular cartilage matrix. There were more cells in the peripheral part of the matrix than in the central part of the matrix. Most of the cells were in cartilaginous lacunae. There were 1 or 2 cells in every cartilaginous lacunae.


CONCLUSIONS
The MSCs can be used as seed cells of tissue engineering and can well proliferate in the acellular cartilage matrix and become tissue engineering cartilage.
Yang
Hongmei
H
Department of Tissue Engineering, Medical University of China, Shenyang, Liaoning, 110001, P. R. China.
Bai
Shuling
S
Wang
Zhijun
Z
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002356:Cartilage / Q000166:cytology; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D018920:Coculture Techniques; D004425:Ear Cartilage / Q000166:cytology*; D005240:Feasibility Studies; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*