title: Fast high-performance liquid chromatography method for quality control of soy extracts.
abstract: Soy extracts contain a mixture of isoflavones belonging to the group of phytoestrogens. In the quality control of soy the amount of isoflavones, both aglycones and glycosides, is usually determined by means of reversed-phase HPLC-UV. On conventional C18-material columns, long analysis times are required in order to separate this complex mixture. In order to speed up analysis, the separation was optimized using two linked monolithic silica-based reversed-phase C18 columns. A spectacular decrease of the analysis time, i.e. almost three-fold, was achieved by applying a flow rate of 3-4 ml/min without loosing any separation efficiency. This analysis method for determination of isoflavones in soy extracts in less than 25 min was fully validated according to the ICH guidelines.
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium. sandra.apers@ua.ac.be
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.forename: Tania
authors.initials: T
authors.lastname: Naessens
authors.forename: Katleen
authors.initials: K
authors.lastname: Van Den Steen
authors.forename: Filip
authors.initials: F
authors.lastname: Cuyckens
authors.forename: Magda
authors.initials: M
authors.lastname: Claeys
authors.forename: Luc
authors.initials: L
authors.lastname: Pieters
authors.forename: Arnold
authors.initials: A
authors.lastname: Vlietinck
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017408:Guidelines as Topic; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D013025:Glycine max / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts