Pancreatic exocrine secretion and plasma concentration of some gastrointestinal hormones in response to abomasal infusion of starch hydrolyzate and/or casein.
Eight Angus steers (290 +/- 8 kg), surgically prepared with pancreatic pouch-duodenal reentrant cannulas and abomasal infusion catheters were used in a replicated 4 x 4 Latin square experiment to investigate the effects of abomasal infusion of starch hydrolyzate (SH) and/or casein on pancreatic exocrine secretion and plasma concentration of hormones. Steers were fed a basal diet of alfalfa (1.2 x NEm) in 12 equal portions daily. Abomasal infusion treatments (6-L total volume infused per day) were water (control), SH [2.7 g/(kg BW x d)], casein [0.6 g/(kg BW x d)], and SH + casein. Periods were 3 d for adaptation and 8 d of full infusion. Pancreatic juice and jugular blood samples were collected over 30-min intervals for 6 h on d 11. Weight and pH of pancreatic samples were measured, and a 10% subsample was composited and frozen until analysis of total protein and pancreatic enzyme activities. The remaining sample was returned to the duodenum. Plasma was harvested and frozen until analyzed. Pancreatic juice (67 mL/h) and protein (1.8 g/h) secretion rates were not affected by nutrient infusion. There were SH x casein interactions for all pancreatic enzyme secretions (U/h; alpha-amylase, P < 0.03; trypsin, P < 0.08; and chymotrypsin, P < 0.03) and plasma insulin concentration (P < 0.10). Secretion of pancreatic enzymes was increased by SH (trypsin) and casein (alpha-amylase, trypsin, and chymotrypsin) but not when SH + casein were infused together. Glucose (P < 0.10) and cholecystokinin octapeptide concentrations (CCK-8; P < 0.05) were increased by SH, but glucagon was decreased (P < 0.10). Casein decreased (P < 0.10) plasma CCK-8 concentrations. These data indicate that positive effects of postruminal casein on enzyme secretion were inhibited by SH, emphasizing the complexity of the regulatory mechanisms involved in dietary adaptation of pancreatic exocrine secretion. Changes in hormone concentration may not relate directly to changes in enzyme secretion.
Swanson
K C
KC
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA.
Benson
J A
JA
Matthews
J C
JC
Harmon
D L
DL
D000018:Abomasum / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism*; D002404:Catheterization / Q000662:veterinary; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D002918:Chymotrypsin / Q000097:blood / Q000378:metabolism; D005934:Glucagon / Q000097:blood; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007328:Insulin / Q000097:blood; D008297:Male; D000455:Medicago sativa; D009929:Organ Size; D046790:Pancreas, Exocrine / Q000201:enzymology / Q000378:metabolism*; D010189:Pancreatic Juice / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000097:blood; D011897:Random Allocation; D012844:Sincalide / Q000097:blood; D013213:Starch / Q000008:administration & dosage / Q000378:metabolism*; D014357:Trypsin / Q000097:blood / Q000378:metabolism; D000516:alpha-Amylases / Q000097:blood / Q000378:metabolism
D001786:Blood Glucose; D002364:Caseins; D007328:Insulin; D010191:Pancreatic Polypeptide; D013213:Starch; D005934:Glucagon; D000516:alpha-Amylases; D002918:Chymotrypsin; D014357:Trypsin; D012844:Sincalide