The delta2 glutamate receptor: a key molecule controlling synaptic plasticity and structure in Purkinje cells.
The orphan glutamate receptor delta2 (GluRdelta2) is predominantly expressed in cerebellar Purkinje cells and plays a crucial role in cerebellar functions; mice that lack the GluRdelta2 gene display ataxia and impaired motor-related learning tasks. However, when expressed alone or with other glutamate receptors, GluRdelta2 does not form functional glutamate-gated ion channels nor does it bind to glutamate analogs. Therefore, the mechanisms by which GluRdelta2 participates in cerebellar functions have been elusive. Studies of mutant mice, such as lurcher, hotfoot, and GluRdelta2 knockout mice, have provided clues to the structure and function of GluRdelta2. Particularly, morphological and electrophysiological analyses of hotfoot and GluRdelta2 knockout mice have indicated a unique role of GluRdelta2 in aligning and maintaining the postsynaptic element with the presynaptic one at parallel fiber (PF)-Purkinje cell synapses. In addition, GluRdelta2 was expressed in newly formed ectopic PF-Purkinje cell synapses found after blockade of electrical activity in adult cerebellum. Moreover, application of an antibody specific for GluRdelta2's extracellular N-terminal region abrogated synaptic plasticity. These results indicate that GluRdelta2 plays a direct role in synapse formation and synaptic plasticity in adult mice. Based on these results, two hypotheses about mechanisms by which GluRdelta2 functions are proposed in this article.
Yuzaki
Michisuke
M
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA. myuzaki@sc.itc.keio.ac.jp
D000818:Animals; D008818:Mice, Neurologic Mutants / Q000502:physiology; D008959:Models, Neurological; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D011689:Purkinje Cells / Q000378:metabolism / Q000502:physiology*; D017470:Receptors, Glutamate / Q000502:physiology*; D013569:Synapses / Q000378:metabolism*
D017470:Receptors, Glutamate; C107913:glutamate receptor delta 2