Trypanosomatid histones.
The histones are responsible for packaging and regulating access to eukaryotic genomes. Trypanosomatids are flagellated protists that diverged early from the eukaryotic lineage and include parasites that cause disease in humans and other mammals. Here, we review the properties of histones in parasitic trypanosomatids, from gene organization and sequence to expression, post-translational modification and function within chromatin. Phylogenetic and experimental analysis indicates that certain specifically conserved histone sequence motifs, particularly within the N-terminal 'tail' domains, possibly represent functionally important modification substrates conserved throughout the eukaryotic lineage. For example, histone H3 contains a highly conserved methylation substrate. Trypanosomatids also possess at least three variant histones. Among these is an orthologue of H2A.Z, a histone involved in protecting 'active' chromatin from silencing in yeast. Histones provide docking platforms for a variety of regulatory factors. The presence of histone modification and variant histones in trypanosomatids therefore represents evidence for a network that provides the discrimination required to regulate transcription, recombination, repair and chromosome replication and segregation.
Alsford
Sam
S
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Horn
David
D
D000818:Animals; D020090:Chromosome Segregation; D017124:Conserved Sequence; D004260:DNA Repair; D004261:DNA Replication; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006657:Histones / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D014351:Trypanosomatina / Q000502:physiology*
D006657:Histones