title: On the course of the irritant reaction after irritation with sodium lauryl sulphate.
abstract: BACKGROUND
  The sodium lauryl sulphate (SLS) irritation test is a well-established model for irritant contact dermatitis after the effects of surfactants.
  
  
  OBJECTIVE
  The course of changes in corneometric measurements (stratum corneum hydration), in transepidermal water loss (TEWL), in laser Doppler measurements (epidermal perfusion) and in colorimetric measurements (skin redness), after a single SLS irritation, should be studied over time.
  
  
  METHODS
  Twenty healthy volunteers were studied. Irritation with 1% SLS solution was applied to the side of one forearm for 24 h under occlusive conditions, while the symmetrical experimental site remained untreated. Measurements were made for 9 days after completion of SLS irritation.
  
  
  RESULTS
  Stratum corneum hydration was reduced immediately after irritation, and subsequently a hyper-hydration was observed. The increase in TEWL, laser Doppler measurements and skin redness persisted for 7-9 days. With regard to skin redness, a mild blanching effect was noticeable after 9 days.
  
  
  CONCLUSIONS
  The evidence of very short-term exsiccation of the stratum corneum with persistent barrier damage, as well as reactive hyper-hydration and blanching of the skin, is remarkable. These first findings suggest that exsiccation of the stratum corneum is not caused by a damage to barrier lipids.
authors.affiliation: Department of Dermatology Municipal Hospital Karlsruhe GmbH, Germany. mgloor@onlinehome.de
authors.forename: Max
authors.initials: M
authors.lastname: Gloor
authors.forename: Bettina
authors.initials: B
authors.lastname: Senger
authors.forename: Marcel
authors.initials: M
authors.lastname: Langenauer
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Fluhr
mesh_terms: D000328:Adult; D001834:Body Water / Q000378:metabolism; D003124:Colorimetry; D017453:Dermatitis, Irritant / Q000209:etiology* / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D005260:Female; D006801:Humans; D007509:Irritants / Q000009:adverse effects*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000098:blood supply / Q000473:pathology; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects*; D013501:Surface-Active Agents / Q000009:adverse effects*; D013997:Time Factors; D014870:Water Loss, Insensible
chemical_list: D007509:Irritants; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate