Docosahexaenoic acid and eicosapentaenoic acid antagonize the proinflammatory interactions of pneumolysin with human neutrophils.
Pneumolysin (4.18 ng/ml)-mediated influx of Ca(2+) and augmentation of the chemoattractant-activated generation of reactive oxidants was antagonized by pretreatment of human neutrophils with the omega-3 polyunsaturated fatty acids docosahexaenoic acid and eicosapentaenoic acid (1.25 to 5 microg/ml). These agents may have potential in attenuating the proinflammatory properties of this pneumococcal toxin.
Cockeran
Riana
R
Medical Research Council for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria, P.O. Box 2034, Pretoria 0001, South Africa. rcockera@medic.up.ac.za
Theron
Annette J
AJ
Feldman
Charles
C
Mitchell
Timothy J
TJ
Anderson
Ronald
R
D001426:Bacterial Proteins; D002118:Calcium / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D013301:Streptolysins / Q000378:metabolism*
D001426:Bacterial Proteins; D016877:Oxidants; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D002118:Calcium