title: Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis.
abstract: A marine green microalga, Platymonas subcordiformis, photo-synthetically generates H(2) but only transiently at a negligible yield when exposed to light after a period of dark anaerobic incubation. A protonophore uncoupler, carbonyl cyanide m-chlorophenylhrazone (CCCP) significantly increased the yield of H(2) photo-production. CCCP optimally at 15 microM gave 4.9 ml H(2) after 8 h light irradiation in 1 l algal cell culture at 1.8 x 10(6) cells ml(-1). The H(2) yield at 15 microM CCCP was increased by 240-fold when compared to the control. This improvement may be by CCCP disrupting the proton motive force thus facilitating proton transfer across the thylakoidal membrane.
authors.affiliation: Marine Bioproducts Engineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Guan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Maicun
authors.initials: M
authors.lastname: Deng
authors.forename: Meifang
authors.initials: M
authors.lastname: Jin
authors.forename: Xingju
authors.initials: X
authors.lastname: Yu
mesh_terms: D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D018462:Photobiology
chemical_list: D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006859:Hydrogen