Differential expression and localization of decorin in human choriodecidual membrane during preterm and term pregnancy.
OBJECTIVE
This study was aimed to isolate cDNAs for genes whose expression levels are dynamically regulated at the feto-maternal interface around parturition.


METHODS
The suppression subtractive hybridization (SSH) method was used to compare the gene expression patterns of human choriodecidual membranes from term elective Caesarian delivery before labor (BL) and term vaginal delivery (VD). Northern blotting, quantitative real-time RT-PCR and in situ hybridization were performed to determine temporal and spatial expression of candidate clones.


RESULTS
After SSH procedure, we selected decorin from the cDNA library of BL for further investigation. The mean level of decorin transcription tended to be higher in BL samples than in VD or preterm samples. Decorin mRNA was mainly expressed in the decidual cells. Decorin gene expression was also upregulated in the term myometrium.


CONCLUSIONS
These results suggest that dynamically increased expression of decorin in the choriodecidual membrane during parturition may be a part of the uterine preparation for labor.
Ogita
Kazuhide
K
Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Suita, Japan.
Kimura
Tadashi
T
Nakamura
Hitomi
H
Koyama
Shinsuke
S
Tsujie
Tomoko
T
Tomiie
Mari
M
Tsutsui
Tateki
T
Shimoya
Koichiro
K
Wada
Yoshinao
Y
Koyama
Masayasu
M
Nomura
Shintaro
S
Murata
Yuji
Y
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D007743:Labor, Obstetric / Q000235:genetics / Q000502:physiology; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
C546878:DCN protein, human; D018076:DNA, Complementary; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger