title: Failure to diagnose recent hepatitis C virus infections in London injecting drug users.
abstract: Hepatitis C virus (HCV) infection results in chronic liver disease in a substantial proportion of those infected. Most new cases of HCV infection in the UK are associated with intravenous drug use. It is important to identify these infections because of the implications for the future health of the individuals concerned and for the control of further spread of infection. However, as hepatitis C infection is characterised by a relatively long asymptomatic period of seronegative viraemia, a laboratory diagnostic protocol that does not test HCV seronegative samples for the presence of HCV RNA may wrongly designate HCV viraemic seronegative individuals as uninfected. Amongst 424 injecting drug users whose serum was sent to our diagnostic laboratory for "HCV screening" over a 14-month period, the prevalence of HCV seropositivity was 48.4%. We retrospectively identified seven individuals for whom there was evidence of recent acquisition of HCV infection. Three of these infections were identified using our routine diagnostic protocol: testing for the presence of HCV-specific antibody and performing HCV RNA testing only on seropositive and seroindeterminate specimens. However, four cases were only identified by HCV RNA testing of HCV seronegative serum. On the basis of these observations, we estimate the incidence of HCV infection amongst London injecting drug users as being 14.3 per 100 person-years. We advocate that all HCV seronegative blood samples obtained from injecting drug users should be tested for the presence of HCV RNA, and suggest that this could be done efficiently by nucleic acid testing the specimens in small pools. .
authors.affiliation: Centre of Virology, University College London, London, United Kingdom.
authors.forename: Emma
authors.initials: E
authors.lastname: Aarons
authors.forename: Paul
authors.initials: P
authors.lastname: Grant
authors.forename: Kate
authors.initials: K
authors.lastname: Soldan
authors.forename: Pete
authors.initials: P
authors.lastname: Luton
authors.forename: Julian
authors.initials: J
authors.lastname: Tang
authors.forename: Richard
authors.initials: R
authors.lastname: Tedder
mesh_terms: D000328:Adult; D003951:Diagnostic Errors; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D015994:Incidence; D008131:London; D008297:Male; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood*; D015819:Substance Abuse, Intravenous / Q000150:complications*; D013997:Time Factors
chemical_list: D018937:Hepatitis C Antibodies; D012367:RNA, Viral