title: [Molecular biological characteristics and genetic significance of Yersinia pestis in China].
abstract: OBJECTIVE
  To understand the molecular biological characteristics in order to analyse the genetic background of Yersinia pestis in China.
  
  
  METHODS
  Primary datum on ribotyping, pulsed field gene electrophoresis (PFGE), random amplified polymorphic DNA (RAPD) and insertion sequence (IS) of Yersinia pestis were used and under cluster analysis. Genetic interval and various methods of recognized molecular feature between different strains were evaluated.
  
  
  RESULTS
  Ribotypes the PFGE types seemed to be corresponding. Stains from Microtus fuscus and area in Tibet Zhongba belonged to 7 copy rRNA gene and the genetic interval were the far more with 6 copy rRNA gene stains, and not definite with RAPD, but with many exceptions. The genetic interval between strains were showed by resemble value.
  
  
  CONCLUSIONS
  Yersinia pestis in China had its own manifold, particular molecular biological characteristics due to natural barriers, geographical complex, circumstances in Tianshan Mountains and Gandise Mountains areas. Yersinia pestis were limited to separateness, evoluted only in certain areas to form a great many gene types.
authors.affiliation: Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hai
authors.forename: Dong-Zheng
authors.initials: DZ
authors.lastname: Yu
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Wei
authors.forename: Lian-Xu
authors.initials: LX
authors.lastname: Xia
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Zhang
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D040961:Genetic Drift; D005838:Genotype; D005843:Geography; D006801:Humans; D051379:Mice; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial