Transamination reactions with multiple turnovers catalyzed by hydrophobic pyridoxamine cofactors in the presence of polyethylenimine polymers.
Pyridoxamines carrying hydrophobic side chains reversibly bind into the hydrophobic core of polyethylenimines and transaminate ketoacids to amino acids with as much as a 725000-fold rate acceleration. Turnover catalysis was achieved by sacrificial oxidative decarboxylation of C-substituted amino acids, which reconverted the pyridoxals to pyridoxamines.
Liu
Lei
L
Department of Chemistry, Columbia University, New York, NY 10027, USA.
Zhou
Wenjun
W
Chruma
Jason
J
Breslow
Ronald
R
D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011733:Pyridoxamine / Q000737:chemistry*; D019289:Pyruvic Acid / Q000737:chemistry; D013379:Substrate Specificity; D000637:Transaminases / Q000737:chemistry*
D011108:Polymers; D011733:Pyridoxamine; D019289:Pyruvic Acid; D011094:Polyethyleneimine; D000637:Transaminases