Individual differences in wheel-running rhythms are related to temporal and spatial patterns of activation of orexin A and B cells in a diurnal rodent (Arvicanthis niloticus).
This study investigated the relationship between the orexins and patterns of activity in the diurnal Nile grass rat, Arvicanthis niloticus. Some individuals of this species switch to a more nocturnal pattern when given access to a running wheel, while others continue to be most active during the day. In both day- and night-active grass rats, the percentages of orexin A (OXA) and orexin B (OXB) cells expressing Fos were highest when animals were actively running in wheels. In night-active animals, removal of the running wheel significantly decreased OXA and OXB cell Fos expression. Additionally, in night-active animals, clear regional differences were apparent. In these animals the presence of a wheel induced higher percentages of Fos in both OXA and OXB cells in medial regions of the lateral hypothalamus than in lateral regions. In night-active animals without access to wheels, this medial-lateral gradient was present only in OXA cells. No regional differences were observed in day-active animals. This study demonstrates that individual differences in the patterns of activation of OXA and OXB cell populations are related to differences in the temporal pattern of wheel running. We also present evidence that orexin cells have projections to the intergeniculate leaflet that appear to make contact with neuropeptide-Y cells. We discuss the possibility that these fibers may be involved in relaying feedback regarding the activity state of the animal to the circadian system through these projections.
Nixon
J P
JP
Department of Zoology, Michigan State University, East Lansing, MI 48824, USA.
Smale
L
L
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology*; D004027:Diencephalon / Q000166:cytology / Q000378:metabolism*; D014644:Genetic Variation / Q000502:physiology*; D005829:Geniculate Bodies / Q000166:cytology / Q000378:metabolism; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D009043:Motor Activity / Q000502:physiology*; D009115:Muridae; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D000068797:Orexins; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism; D014851:Wakefulness / Q000502:physiology
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D000068797:Orexins; D016760:Proto-Oncogene Proteins c-fos