Joule heating and determination of temperature in capillary electrophoresis and capillary electrochromatography columns.
This article reviews the progress that has taken place in the past decade on the topic of estimation of Joule heating and temperature inside an open or packed capillary in electro-driven separation techniques of capillary electrophoresis (CE) and capillary electrochromatography (CEC), respectively. Developments in theoretical modeling of the heat transfer in the capillary systems have focused on attempts to apply the existing models on newer techniques such as CEC and chip-based CE. However, the advent of novel analytical tools such as pulsed magnetic field gradient nuclear magnetic resonance (NMR), NMR thermometry, and Raman spectroscopy, have led to a revolution in the area of experimental estimation of Joule heating and temperature inside the capillary via the various noninvasive techniques. This review attempts to capture the major findings that have been reported in the past decade.
Rathore
Anurag S
AS
Process Development, Amgen Inc., 30 W-2-A, One Amgen Center Drive, Thousand Oaks, CA 91320, USA. arathore@amgen.com
D002845:Chromatography / Q000379:methods*; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D013696:Temperature