Innovative instrumentation for microarray scanning and analysis: application for characterization of oligonucleotide duplexes behavior.
Accuracy in microarray technology requires new approaches to microarray reader development. A microarray reader system (optical scanning array or OSA reader) based on automated microscopy with large field of view, high speed 3 axis scanning at multiple narrow-band spectra of excitation light has been developed. It allows fast capture of high-resolution, multi-fluorescence images and is characterized by a linear dynamic range and sensitivity comparable to commonly used photo-multiplier tube (PMT)-based laser scanner. Controlled by high performance software, the instrument can be used for scanning and quantitative analysis of any type of dry microarray. Studies implying temperature-controlled hybridization chamber containing a microarray can also be performed. This enables the registration of kinetics and melting curves. This feature is required in a wide range of on-chip chemical and enzymatic reactions including on-chip PCR amplification. We used the OSA reader for the characterization of hybridization and melting behaviour of oligonucleotide:oligonucleotide duplexes on three-dimensional Code Link slides.
Khomyakova
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EB
IMSTAR, 60, rue Notre Dame des Champs, 75006 Paris, France. elena.khomiakova@imstar.fr
Dreval
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EV
Tran-Dang
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M
Potier
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MC
Soussaline
F P
FP
D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D012984:Software
D009841:Oligonucleotides