title: Characterization of mutations affecting embryonic hematopoiesis in the medaka, Oryzias latipes.
abstract: In a genetic screen for mutations affecting organogenesis in the medaka, Oryzias latipes, we identified eight mutants with defects in embryonic hematopoiesis. These mutations were classified into seven complementation groups. In this paper, we characterize the five mutants that were confirmed in the next generation. The beni fuji mutant was defective in the generation of blood cells, exhibiting reduced blood cells at the initiation of circulation. Mutations in two genes, lady finger and ryogyoku, caused abnormal morphology of blood cells, i.e., deformation, along with a progressive decrease in the number of blood cells. The sekirei mutant exhibited photosensitivity with autofluorescent blood cells. Mutations in kyoho resulted in huge blood cells that were approximately three times longer than the wild-type blood cells. The spectrum of phenotypes identified in this study is similar to that of the zebrafish hematopoietic mutants except for the huge blood cells in kyoho. Our results demonstrate that medaka, as well as zebrafish, is a useful model to study hematopoiesis.
authors.affiliation: Department of Biological Information, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohisa
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Orihara
authors.forename: Sae
authors.initials: S
authors.lastname: Sakaguchi
authors.forename: Kyohei
authors.initials: K
authors.lastname: Horie
authors.forename: Kenta
authors.initials: K
authors.lastname: Hibiya
authors.forename: Sayaka
authors.initials: S
authors.lastname: Konno
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Miyake
authors.forename: Davin
authors.initials: D
authors.lastname: Setiamarga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeda
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Imai
authors.forename: Akira
authors.initials: A
authors.lastname: Kudo
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D006410:Hematopoiesis / Q000235:genetics* / Q000502:physiology; D009154:Mutation; D009990:Oryzias / Q000196:embryology* / Q000235:genetics* / Q000502:physiology