Effects of delipidation and oxygen concentration on in vitro development of porcine embryos.
The effects of delipidation and the oxygen (O(2)) concentration in the atmosphere during culture on in vitro development and H(2)O(2) content were investigated in porcine in vivo fertilized embryos and embryos after in vitro maturation and in vitro fertilization (IVM/IVF embryos). There was no significant difference in the developmental rates to the blastocyst stage between the intact and delipidated IVM/IVF embryos. However, the mean number of cells in blastocysts derived from delipidated IVM/IVF embryos (19.8 +/- 0.8 cells) was significantly smaller than that from intact embryos (24.2 +/- 1.2 cells). Although there were no significant differences in the developmental rates to the blastocyst stage of intact and delipidated IVM/IVF embryos between the cultures under 5% O(2) and 20% O(2), the developmental rate of intact IVM/IVF embryos cultured under 5% O(2) (27.1%) was significantly higher than that of the delipidated embryos cultured under 20% O(2) (19.3%). On the other hand, there was no difference in the developmental rate to the blastocyst stage between in vivo fertilized embryos cultured under 5% O(2) and 20% O(2). Hydrogen peroxide (H(2)O(2)), one of the reactive oxygen species (ROS), is thought to cause damage to embryos. The H(2)O(2) content per embryo derived from oocytes cultured under 5% O(2) (in vivo fertilized, 58.0 +/- 2.5 pixels; IVM/IVF, 79.6 +/- 3.2 pixels) was significantly lower than that (in vivo fertilized, 100.2 +/- 3.8 pixels; IVM/IVF, 103.9 +/- 3.2 pixels) under 20% O(2). Furthermore, the level of H(2)O(2) in delipidated IVM/IVF embryos (94.7 +/- 3.9 pixels) was significantly lower than that in intact embryos (103.9 +/- 3.2 pixels) cultured under 20% O(2). The present results indicate that the delipidation of porcine IVM/IVF embryos and reduction of the O(2) concentration decreased the H(2)O(2) level rather than the in vitro developmental rate to the blastocyst stage.
Yoneda
Akihiro
A
Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. ayoneda@anim.agr.hokudai.ac.jp
Suzuki
Keita
K
Mori
Tadashi
T
Ueda
Junji
J
Watanabe
Tomomasa
T
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D009865:Oocytes / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D013997:Time Factors
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010100:Oxygen