title: [The expression and activity detection of a variant N protein of SARS-CoV].
abstract: OBJECTIVE
  To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli.
  
  
  METHODS
  The N region gene of SARS-CoV was cloned by RT-PCR. The expression vector was constructed by DNA recombination. The recombinant plasmid was transformed into E.coli BL21(DE3). The expression of the fusion protein was detected by Western blot.
  
  
  RESULTS
  (1) As compared with the sequences in GenBank, 20 bp were deleted in DNA sequence of the cloned N protein. (2) The fusion protein GST-N was soluble. Western blot analysis showed that the reaction of GST-N to anti-SARS-CoV sera was positive.
  
  
  CONCLUSIONS
  The pGEX-2T/N has been constructed and expressed in the form of fusion protein GST-N successfully, which lays the foundation for further study of SARS-CoV N protein.
authors.affiliation: Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Qi
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Cui-li
authors.initials: CL
authors.lastname: Shu
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D001483:Base Sequence; D000086462:Coronavirus Nucleocapsid Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase