title: The IkappaB kinase complex and NF-kappaB act as master regulators of lipopolysaccharide-induced gene expression and control subordinate activation of AP-1.
abstract: Toll-like receptors (TLRs) recognize conserved products of microbial pathogens to initiate the innate immune response. TLR4 signaling is triggered upon binding of lipopolysaccharides (LPS) from gram-negative bacteria. Using comparative gene expression profiling, we demonstrate a master regulatory role of IkappaB kinase (IKK)/NF-kappaB signaling for immediate-early gene induction after LPS engagement in precursor B cells. IKK/NF-kappaB signaling controls a large panel of gene products associated with signaling and transcriptional activation and repression. Intriguingly, the induction of AP-1 activity by LPS in precursor B cells and primary dendritic cells fully depends on the IKK/NF-kappaB pathway, which promotes expression of several AP-1 family members, including JunB, JunD, and B-ATF. In pre-B cells, AP-1 augments induction of a subset of primary NF-kappaB targets, as shown for chemokine receptor 7 (CCR7) and immunoglobulin kappa light chain. Thus, our data illustrate that NF-kappaB orchestrates immediate-early effects of LPS signaling and controls secondary AP-1 activation to mount an appropriate biological response.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Berlin, Germany. dkrapp@mdc-berlin.de
authors.forename: Daniel
authors.initials: D
authors.lastname: Krappmann
authors.forename: Elmar
authors.initials: E
authors.lastname: Wegener
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sunami
authors.forename: Meral
authors.initials: M
authors.lastname: Esen
authors.forename: Andreas
authors.initials: A
authors.lastname: Thiel
authors.forename: Benjamin
authors.initials: B
authors.lastname: Mordmuller
authors.forename: Claus
authors.initials: C
authors.lastname: Scheidereit
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D017781:Genes, Immediate-Early; D006801:Humans; D051550:I-kappa B Kinase; D008070:Lipopolysaccharides / Q000494:pharmacology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D008070:Lipopolysaccharides; D046911:Macromolecular Substances; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D017346:Protein Serine-Threonine Kinases; C496559:CHUK protein, human; D051550:I-kappa B Kinase; C496560:IKBKB protein, human; C496565:IKBKE protein, human