Three-dimensional microarray platform applied to single nucleotide polymorphism analysis.
Bio-Strand, Inc., has developed a novel DNA microarray platform utilizing a three-dimensional (3D) DNA format. DNA probes or polymerase chain reaction (PCR) products are spotted onto a thread-like scaffold, which is then wound onto a cylindrical core. By wrapping the thread around the core, high efficiencies are achieved in sample analysis. Using allele-specific oligo (ASO) competitive hybridization (with Cy5 fluorescently labeled sequences), hybridized arrays are visualized using a helium-neon (HeNe) laser and quantitated/scored. The method can readily detect single nucleotide differences. We demonstrate the use of this Bio-Strand 3D array in the analysis of a single nucleotide polymorphism (SNP).
Stimpson
Donald I
DI
Bio-Strand Inc., 1258 Quarry Lane Suite H, Pleasanton, CA 94588, USA.
Knepper
Sheila M
SM
Shida
Miho
M
Obata
Kimimichi
K
Tajima
Hideji
H
D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D006801:Humans; D009757:Nylons / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity
D002232:Carbocyanines; D015342:DNA Probes; D009757:Nylons; C085321:cyanine dye 5; D004247:DNA