Measurement of polychlorinated biphenyls in fish tissue by gas chromatography with electron capture detection and enzyme-linked immunosorbent assay.
Polychlorinated biphenyls in fish tissue were analyzed by enzyme-linked immunosorbent assay (ELISA) and gas chromatography with electron capture detection (GC/ECD) methods. Fish samples were collected in 2000 and 2001 during an Indiana fish survey. For samples with concentrations ranging from 0.05 to 0.5 ppm (n = 40) and 0.5 to 5.0 ppm (n = 12), the ELISA results were not significantly different from GC/ECD (P < 0.05). This research has demonstrated the effectiveness of analysis of fish samples by ELISA in the development of fish consumption advisories that accurately articulate health risks from the consumption of contaminated fish.
Lasrado
J A
JA
Departament of Foods and Nutrition, Purdue University, Stone Hall, Room 205, 700 West State Street, West Lafayette, Indiana 47907-2059, USA.
Santerre
C R
CR
Stahl
J R
JR
Noltemeyer
T
T
Deardorff
D
D
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D003257:Consumer Product Safety; D004785:Environmental Pollutants; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D014020:Tissue Extracts / Q000032:analysis / Q000737:chemistry
D004785:Environmental Pollutants; D014020:Tissue Extracts; D011078:Polychlorinated Biphenyls