Evaluation of an automated immunomagnetic separation method for the rapid detection of Salmonella species in poultry environmental samples.
Automated immunomagnetic separation (AIMS), using Dynabeads anti-Salmonella (Dynal, Oslo), was evaluated for its ability to detect Salmonella spp. in poultry environmental samples in comparison with standard, culture-based method (Health Canada, Health Protection Branch, MFHPB-20). AIMS was found to be more reliable in detecting Salmonella from artificially inoculated enrichment broths at low levels and exhibited a 15.5% higher sensitivity value than the culture method.
Lynch
Michael J B
MJ
Laboratory Services Division, University of Guelph, 95 Stone Road West, Guelph, Ontario, Canada.
Leon-Velarde
Carlos G
CG
McEwen
Scott
S
Odumeru
Joseph A
JA
D000818:Animals; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D005516:Food Microbiology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods* / Q000706:statistics & numerical data; D011200:Poultry / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification* / Q000472:pathogenicity; D012478:Salmonella Food Poisoning / Q000517:prevention & control; D012680:Sensitivity and Specificity