title: Transient increase in anti-p-ATF2 immunoreactivity in the late secretion ameloblasts apical to the transition zone of rat incisors.
abstract: Activating transcription factor 2 (ATF2) was localized in the ameloblasts of rat incisors by immunohistochemistry. A specific antibody against phosphorylated ATF2 (p-ATF2), which is an activated form of ATF2, was detected from the proliferation zone to maturation ameloblasts just after the transition. In the secretion zone, a transient increase in p-ATF2 was observed in the late secretion ameloblast nuclei, where a stronger reactivity of p-ATF2 extended from 1 mm apical to the transition to the transition zone, whereas ameloblast nuclei in most of the maturation zone exhibited either weak or no reactivity. A similar pattern was also observed in the case of c-Jun immunohistochemistry, except for in most of the maturation zone, where strong c-Jun reactivity was detected. Thus, ATF-2 and c-Jun are deeply involved in amelogenesis and, in particular, ATF2 is related to the proliferation, differentiation, secretion and transition zones.
authors.affiliation: Department of Biology, Tsururmi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. nishikawa-s@tsurumi-u.ac.jp
authors.forename: Sumio
authors.initials: S
authors.lastname: Nishikawa
mesh_terms: D051698:Activating Transcription Factor 2; D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003743:Dental Enamel / Q000378:metabolism; D007150:Immunohistochemistry; D007180:Incisor / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D051698:Activating Transcription Factor 2; C497107:Atf2 protein, rat; D017362:Cyclic AMP Response Element-Binding Protein; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors