title: Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa).
abstract: We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A.
authors.affiliation: Department of Bioengineering, Akita Research Institute of Food and Brewing, Akita, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiwatashi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hori
authors.forename: Keitaro
authors.initials: K
authors.lastname: Takahashi
authors.forename: Akira
authors.initials: A
authors.lastname: Kagaya
authors.forename: Shunzo
authors.initials: S
authors.lastname: Inoue
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Saori
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D019281:Dimerization; D004151:Dipeptides / Q000378:metabolism; D004791:Enzyme Inhibitors; D048691:Grifola / Q000737:chemistry / Q000201:enzymology*; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D004151:Dipeptides; D004791:Enzyme Inhibitors; D000626:Aminopeptidases; C022450:prolyl aminopeptidase