Erythropoietin/erythropoietin-receptor system as an angiogenic factor in chemically induced murine hepatic tumors.
BACKGROUND
To clarify the role of erythropoietin (Epo) in hepatic tumor angiogenesis, expression of Epo and its receptor (Epo-R) and content of Epo were investigated in murine chemically induced hepatic tumors.


METHODS
To induce hepatic tumors and cirrhosis, diaminobenzidine was administered to Wistar rats for 5 months. In total, 30 hepatic tumors of greater than 3 mm in diameter were induced in 12 rats. The 30 hepatic tumors were resected with the surrounding hepatic tissues. The Epo content was measured by a radioimmunoassay (RIA) method. The number of tumor vessels in a definite area was counted in 100 areas of each tumor. To demonstrate the expression of Epo-R in tumors or surrounding liver tissues, immunohistochemical staining for Epo-R was performed.


RESULTS
The Epo content of tumors ranged from 6.1 to 97.8 mU/ml, with a median of 21.8 mU/ml, which was significantly higher than that of the cirrhotic tissues adjacent to the tumors. Epo was not detectable in the normal or cirrhotic liver tissues without tumors. A significant correlation between Epo content and vascular density was noted in the 30 hepatic tumors (correlation coefficient, 0.480; P = 0.01). Immunoreactive Epo-R was detectable in the endothelium of intervening vessels of all hepatic tumors examined.


CONCLUSIONS
The Epo/Epo-R system is related to the angiogenesis of murine hepatic tumors. To clarify the role of erythropoietin (Epo) in hepatic tumor angiogenesis, expression of Epo and its receptor (Epo-R) and content of Epo were investigated in murine chemically induced hepatic tumors.
Nakamatsu
Kiyoshi
K
Department of Radiology, Kinki University School of Medicine, 77-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan. kiyoshi@med.kindai.ac.jp
Nishimura
Yasumasa
Y
Suzuki
Minoru
M
Kanamori
Shuichi
S
Maenishi
Osamu
O
Yasuda
Yoshiko
Y
D000818:Animals; D002196:Capillaries / Q000737:chemistry; D004921:Erythropoietin / Q000032:analysis / Q000502:physiology*; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000098:blood supply* / Q000139:chemically induced / Q000737:chemistry; D008297:Male; D008749:Methyldimethylaminoazobenzene; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000503:physiopathology*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D017467:Receptors, Erythropoietin / Q000032:analysis / Q000502:physiology*
D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D008749:Methyldimethylaminoazobenzene