mRNA expression of tachykinins and tachykinin receptors in different human tissues.
The tachykinins substance P, neurokinin A and neurokinin B are involved in many pathophysiological processes. A reverse transcription-polymerase chain reaction (RT-PCR) assay was used to analyse the expression of TAC1 and TAC3, the genes that encode substance P/neurokinin A and neurokinin B, respectively, and the genes encoding the tachykinin NK(1), NK(2) and NK(3) receptors in different human tissues. The data show that tachykinins and their receptors mRNAs are broadly distributed in different human tissues being present in neuronal and non-neuronal types of cells. The presence of TAC3 and the tachykinin NK(3) receptor (TACR3) in a wide variety of peripheral tissues argue for a still unexplored role of this ligand-receptor pair in mediating visceral effects of tachykinins. We found, for the first time, that TAC3 and TACR3 mRNAs are expressed in human airways and pulmonary arteries and veins, providing further evidence for the involvement of this system in lung physiopathology.
Pinto
Francisco M
FM
Instituto de Investigaciones Químicas, Centro de Investigaciones Científicas Isla de la Cartuja, CSIC, Avda. Americo Vespucio s/n, 41092 Sevilla, Spain.
Almeida
Teresa A
TA
Hernandez
Mariano
M
Devillier
Philippe
P
Advenier
Charles
C
Candenas
M Luz
ML
D000199:Actins / Q000096:biosynthesis; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis*; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis; D018041:Receptors, Neurokinin-2 / Q000096:biosynthesis; D018042:Receptors, Neurokinin-3 / Q000096:biosynthesis; D018039:Receptors, Tachykinin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015320:Tachykinins / Q000096:biosynthesis*; D014018:Tissue Distribution
D000199:Actins; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D018042:Receptors, Neurokinin-3; D018039:Receptors, Tachykinin; D015320:Tachykinins