title: Factors other than phytosterols in some vegetable oils affect the survival of SHRSP rats.
abstract: Unusual survival-shortening activities of some vegetable oils were detected in stroke-prone spontaneously hypertensive (SHRSP) rats, and phytosterol (PS) in the oils and the tissue tocopherol status have been suggested to be the factors for the activities. Here, we re-evaluated the contribution of PS to the survival-shortening, and examined the hepatic tocopherol status. A basal diet for rodents and a test oil were mixed at a 9:1 ratio, and the diet was given to male SHRSP rats upon weaning. The total and major PS contents of the diets and tissue lipids did not correlate with relative survival time. The free fatty acid fractions obtained by lipase and alkaline hydrolyses of canola oil (Can) and the original Can contained PS in comparable amounts but the free fatty acid fractions did not exhibit survival-shortening activities compared with the soybean oil (Soy) group. The activity was not detected in the ethyl acetate extracts of the aqueous phase after the hydrolysis. When a commercially available PS preparation was added to the Soy diet at an amount 2.8-fold higher than that in the Can diet, the mean survival time was shortened but was still significantly longer than that of the Can group. The hepatic tocopherol level was significantly higher in the Can group than in the hydrogenated Soy group and Soy group, but the former two groups exhibited a survival-shortening activity. These results indicate that factors other than PS, tocopherol status and fatty acid composition in some vegetable oils are critical for the survival-shortening activity observed in SHRSP rats.
authors.affiliation: Department of Preventive Nutraceutical Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuhoku, Nagoya 467-8603, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tatematsu
authors.forename: Shin-Ya
authors.initials: SY
authors.lastname: Fuma
authors.forename: Tomoya
authors.initials: T
authors.lastname: Nagase
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Fujii
authors.forename: Harumi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage* / Q000737:chemistry; D006973:Hypertension / Q000150:complications / Q000401:mortality*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008136:Longevity / Q000187:drug effects; D008297:Male; D010840:Phytosterols / Q000008:administration & dosage* / Q000032:analysis; D000074262:Rapeseed Oil; D051381:Rats; D011918:Rats, Inbred SHR; D013024:Soybean Oil / Q000008:administration & dosage* / Q000737:chemistry; D020521:Stroke / Q000209:etiology / Q000401:mortality*; D015996:Survival Rate; D024505:Tocopherols / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D010840:Phytosterols; D000074262:Rapeseed Oil; D013024:Soybean Oil; D024505:Tocopherols