title: Human immunodeficiency virus type 1-specific immune responses in primates upon sequential immunization with adenoviral vaccine carriers of human and simian serotypes.
abstract: Two triple immunization vaccine regimens with adenoviral vectors with E1 deleted expressing Gag of human immunodeficiency virus type 1 were tested for induction of T- and B-cell-mediated-immune responses in mice and in nonhuman primates. The vaccine carriers were derived from distinct serotypes of human and simian adenoviruses that fail to elicit cross-neutralizing antibodies expected to dampen the effect of booster immunizations. Both triple immunization regimens induced unprecedented frequencies of gamma interferon-producing CD8(+) T cells to Gag in mice and monkeys that remained remarkably stable over time. In addition, monkeys developed Gag-specific interleukin-2-secreting T cells, presumably belonging to the CD4(+) T-cell subset, and antibodies to both Gag and the adenoviral vaccine carriers.
authors.affiliation: Wistar Institute, Philadelphia, PA 19104, USA.
authors.forename: A
authors.initials: A
authors.lastname: Reyes-Sandoval
authors.forename: J C
authors.initials: JC
authors.lastname: Fitzgerald
authors.forename: R
authors.initials: R
authors.lastname: Grant
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Xiang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: G P
authors.initials: GP
authors.lastname: Gao
authors.forename: J M
authors.initials: JM
authors.lastname: Wilson
authors.forename: H C J
authors.initials: HC
authors.lastname: Ertl
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007115:Immunization Schedule; D007117:Immunization, Secondary; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D019076:Transgenes
chemical_list: D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies