Phan3D: design of biological phantoms in 3D electron microscopy.
Three-Dimensional Electron Microscopy (3DEM) has turned out to be a major player in structural proteomics. In 3DEM, tens of thousands of EM images from a macromolecule at different views are combined to derive its structure by means of a 3D reconstruction algorithm. However, for an optimal reconstruction, the most suitable algorithm has to be used and its parameters have to be tuned for the macromolecule under study and the experimental conditions found. The use of phantoms is central to objective comparison of reconstruction algorithms and optimization of their parameters. Phan3D is a tool intended to provide a fully visual and interactive environment that facilitates the design of phantoms resembling biological specimens in 3DEM. Phan3D has been developed as an alternative to the tedious, error-prone and old-fashioned phantom design that is based on hand-written text description files.
Bilbao-Castro
J R
JR
Centro Nacional Biotecnologia, Universidad Autonoma, 28049 Madrid, Spain.
Sorzano
C O S
CO
García
I
I
Fernández
J J
JJ
D003196:Computer Graphics; D003198:Computer Simulation; D017076:Computer-Aided Design; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D008958:Models, Molecular; D019047:Phantoms, Imaging; D011506:Proteins / Q000648:ultrastructure; D012984:Software; D014584:User-Computer Interface
D011506:Proteins