Mutagenic insertion and chromosome engineering resource (MICER).
Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.
Adams
David J
DJ
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK.
Biggs
Patrick J
PJ
Cox
Tony
T
Davies
Rob
R
van der Weyden
Louise
L
Jonkers
Jos
J
Smith
James
J
Plumb
Bob
B
Taylor
Ruth
R
Nishijima
Ichiko
I
Yu
Yuejin
Y
Rogers
Jane
J
Bradley
Allan
A
D000818:Animals; D015723:Gene Library; D020868:Gene Silencing; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D011995:Recombination, Genetic; D017384:Sequence Deletion