Biosensors for detection of mercury in contaminated soils.
Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities.
Bontidean
Ibolya
I
Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Mortari
Alessia
A
Leth
Suzanne
S
Brown
Nigel L
NL
Karlson
Ulrich
U
Larsen
Martin M
MM
Vangronsveld
Jaco
J
Corbisier
Philippe
P
Csöregi
Elisabeth
E
D015374:Biosensing Techniques / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D008628:Mercury / Q000032:analysis*; D027805:Phaseolus / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis*
D012989:Soil Pollutants; D008628:Mercury