Visualization of microarray results to assist interpretation.
Whole genome microarrays allow assessment of the profile of genes expressed under particular experimental conditions, including external stimuli such as pH or temperature, and internal changes brought about by deleting or over-expressing a gene. Such experiments produce large data sets, for which sophisticated analysis software is available. What is lacking are tools for analysing data sets from different experiments, in order to test and generate hypotheses about the links between regulatory networks. We describe here a method for presenting results from different experiments as a directed graph constructed using an automated graph drawing program xneato, enhanced by a logic program designed to cluster data and aid in the generation of hypotheses about possible gene interactions. A web-based front-end to the system has been constructed to explore and manipulate the graphical displays produced. Results of microarray experiments on Mycobacterium tuberculosis were used to develop and evaluate the visualization tool and initiate the development of an inference system for gene interactions based on such data. The GeneGraph project can be accessed at: zebrafish.doc.ic.ac.uk
Papatheodorou
Irene
I
Department of Computing, Imperial College London, SW7 2AZ, UK.
Sergot
Marek
M
Randall
Marty
M
Stewart
Graham R
GR
Robertson
Brian D
BD
D003196:Computer Graphics; D004269:DNA, Bacterial / Q000235:genetics; D004777:Environment; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020407:Internet; D005810:Multigene Family; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012984:Software
D004269:DNA, Bacterial