Developing ovarian follicles inhibit the endotoxin-induced glomerular inflammatory reaction in pseudopregnant rats.
OBJECTIVE
We tested the hypothesis that developing ovarian follicles produce factors inhibiting the endotoxin induced inflammatory response.


METHODS
Pseudopregnant rats were treated with FSH to induced follicular development (FSH-rats). For control we used untreated pseudopregnant rats (PSP-rats) and rats in the follicular phase of the ovarian cycle (C-rats). All rats were infused with either saline or endotoxin. Three days after the infusion rats were sacrificed and kidney specimens were snapfrozen. Cryostat kidney sections were stained for the presence of monocytes, granulocytes, CD11a- and CD11b-positive cells and for ICAM-1 expression.


RESULTS
The results show that induction of follicular development in pseudopregnant rats inhibited glomerular infiltration of monocytes and CD11b(+) cells, while it did not affect the other parameters, i.e. glomerular granulocyte number, CD11a(+) cells and glomerular ICAM-1 expression.


CONCLUSIONS
Developing ovarian follicles produce factors inhibiting monocyte responses to endotoxin.
Faas
Marijke M
MM
Transplantation Biology and Immunoendocrinology, Division of Medical Biology, Department of Pathology and Laboratory Medicine, University of Groningen, The Netherlands. m.m.faas@med.rug.nl
van der Schaaf
Gerda
G
Moes
Henk
H
Heineman
Maas Jan
MJ
de Vos
Paul
P
D000818:Animals; D040881:CD11a Antigen / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count; D004731:Endotoxins / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005498:Follicular Phase / Q000502:physiology; D005921:Glomerulonephritis / Q000139:chemically induced / Q000473:pathology*; D006098:Granulocytes / Q000166:cytology; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009000:Monocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000254:growth & development / Q000502:physiology*; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar
D040881:CD11a Antigen; D039481:CD11b Antigen; D004731:Endotoxins; D018799:Intercellular Adhesion Molecule-1; D011374:Progesterone; C044101:endotoxin, Escherichia coli; D005640:Follicle Stimulating Hormone