Determination of the peptide binding motif and high-affinity ligands for HLA-DQ4 using synthetic peptide libraries.
Juvenile idiopathic arthritis (JIA) is considered to be an autoimmune disease. Various human leukocyte antigen (HLA) associations for different subgroups of this heterogeneous disease have been found. For early-onset pauciarticular arthritis (now oligoarthritic JIA), a strong association with the HLA class II haplotype DQA1*0401/DQB1*0402 (DQ4) has been described. We determined the peptide-binding specificities of this HLA-DQ molecule by screening a synthetic acetylated nonapeptide amide library with one defined and eight random sequence positions. A characteristic binding motif could be deduced. By use of these data, we designed defined specific nonapeptides and identified high-affinity ligands binding to HLA-DQ4. The peptide binding motif of HLA-DQ4 is very similar to the motif of HLA-DQ7, also associated with oligoarthritic JIA. It is, however, different from binding motifs of neutral or protective HLA-DQ molecules. Our results further support the idea of differential peptide presentation in the pathogenesis of oligoarthritic JIA.
Volz
Thomas
T
University Children's Hospital, Tuebingen, Germany.
Schwarz
Gerold
G
Fleckenstein
Burkhard
B
Schepp
Carsten P
CP
Haug
Markus
M
Roth
Johannes
J
Wiesmüller
Karl-Heinz
KH
Dannecker
Günther E
GE
D020816:Amino Acid Motifs / Q000276:immunology; D000595:Amino Acid Sequence; D001171:Arthritis, Juvenile / Q000276:immunology* / Q000473:pathology; D002461:Cell Line, Transformed; D006683:HLA-DQ Antigens / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology
D006683:HLA-DQ Antigens; C060029:HLA-DQ4 antigen; C069546:HLA-DQ7 antigen; D008024:Ligands; D019151:Peptide Library; D010455:Peptides