title: An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.
abstract: MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states.
authors.affiliation: Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Liu
authors.forename: George Adrian
authors.initials: GA
authors.lastname: Calin
authors.forename: Brian
authors.initials: B
authors.lastname: Meloon
authors.forename: Nir
authors.initials: N
authors.lastname: Gamliel
authors.forename: Cinzia
authors.initials: C
authors.lastname: Sevignani
authors.forename: Manuela
authors.initials: M
authors.lastname: Ferracin
authors.forename: Calin Dan
authors.initials: CD
authors.lastname: Dumitru
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Simona
authors.initials: S
authors.lastname: Zupo
authors.forename: Mariella
authors.initials: M
authors.lastname: Dono
authors.forename: Hansjuerg
authors.initials: H
authors.lastname: Alder
authors.forename: Florencia
authors.initials: F
authors.lastname: Bullrich
authors.forename: Massimo
authors.initials: M
authors.lastname: Negrini
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
mesh_terms: D000328:Adult; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005333:Fetus / Q000378:metabolism; D020869:Gene Expression Profiling; D016678:Genome; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009369:Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D009928:Organ Specificity; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes