Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry.
A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the diode array detector and atmospheric pressure chemical ionization (APCI) mass spectrometer. More than 52 components in the methanol extract of R. chuanxiong were resolved and 11 of them were preliminary identified according to their UV and mass spectra.
Chen
Xueguo
X
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 161, Zhongshan Road, Dalian 116011, China.
Kong
Liang
L
Su
Xingye
X
Fu
Hongjing
H
Ni
Jianyi
J
Zhao
Ruihuan
R
Zou
Hanfa
H
D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal