title: [Using the FRTL-5 cell to screen the thyroxine disrupting effects of the two pesticides-ethylenethiourea and pendimethalin].
abstract: OBJECTIVE
  In order to study the effects of ethylenethiourea(ETU) and pendimethalin on synthesis and secretion of thyroglobulin(Tg) in FRTL-5 cells in vitro.
  
  
  METHODS
  MTT test was used to investigate the cytotoxicity of the two chemicals to FRTL-5 cell. When the concentration of pendimethalin exceeded 5 ng/microliter, and the concentration of ethylenethiourea exceeded 360 ng/microliter, there was distinct cytotoxicity to FRTL-5 cell. The radioimmunoassay was also used to detect the level of thyroglobulin in culture. The exposure doses in radioimmunoassay was based on the MTT test.
  
  
  RESULTS
  The concentration of Tg was decreased in medium and high dose of pendimethalin and ethylenethiourea.
  
  
  CONCLUSIONS
  It is likely, therefore, that the two pesticides might disrupt FRTL-5 cell of synthesizing and secreting Tg.
authors.affiliation: Huaxi School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Pan
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.forename: Jintao
authors.initials: J
authors.lastname: Wang
authors.forename: Desheng
authors.initials: D
authors.lastname: Wu
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005031:Ethylenethiourea / Q000633:toxicity*; D005659:Fungicides, Industrial / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity; D051381:Rats; D012680:Sensitivity and Specificity; D013954:Thyroglobulin / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D013974:Thyroxine / Q000096:biosynthesis*
chemical_list: D000814:Aniline Compounds; D005659:Fungicides, Industrial; D006540:Herbicides; D005031:Ethylenethiourea; D013954:Thyroglobulin; D013974:Thyroxine; C030856:pendimethalin