The Mycobacterium tuberculosis dosRS two-component system is induced by multiple stresses.
Induction of the Mycobacterium tuberculosis dosR gene, which is known to respond to hypoxia, was measured using RTq-PCR following exposure to different stresses. Increased expression was seen after exposure to S-nitrosoglutathione (GSNO), ethanol and (to a lesser extent) H2O2, but not heat- or cold-shock. We also demonstrated that hspX, which is dependent on dosR for expression, is induced when cultures are left standing for 30 min, while significant but minor induction was seen following a 10 min centrifugation. Microarray analysis was used to compare gene expression in wild-type and deltadosR strains following 30 min standing. Fifty-two genes were significantly up-regulated, and 19 genes were down-regulated. These included genes that had previously been reported as being part of the dosR regulon, and also some novel ones.
Kendall
S L
SL
Department of Pathology and Infectious Diseases, Royal Veterinary College, London NW1 0TU, UK.
Movahedzadeh
F
F
Rison
S C G
SC
Wernisch
L
L
Parish
T
T
Duncan
K
K
Betts
J C
JC
Stoker
N G
NG
D004269:DNA, Bacterial / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D026422:S-Nitrosoglutathione / Q000494:pharmacology
D004269:DNA, Bacterial; D000431:Ethanol; D026422:S-Nitrosoglutathione; D006861:Hydrogen Peroxide