title: Effect of oral gavage dosing regimens in female Tg rasH2 transgenic mice.
abstract: Female Tg rasH2 (CB6F1/Jic-TgrasH2@Tac) mice were administered water once daily, water twice daily with 8 or 12 hours between doses, 1% sodium dodecyl sulfate in water (1% SDS) once daily, or 1% SDS twice daily with 12 hours between doses by oral gavage at volumes of 10 ml/kg/day for 28 or 29 consecutive days. A control group of mice received no treatment and no sham manipulation. There were no significant differences in body weight or food consumption between treated groups and untreated control mice. Mean weights of spleens, livers, and thymuses were lower than control values in most groups of mice subjected to gavage. Focal or multifocal loss of thymic cortical architecture was observed in 13 of 50 mice distributed among all groups (including naïve controls), however only in one instance was this finding suggestive of a precursor to neoplasia. This study demonstrated that Tg rasH2 mice can tolerate once or twice daily gavage dosing with water or vehicle containing 1% SDS. Loss of thymic cortical architecture was a common incidental finding in female Tg rasH2 mice.
authors.affiliation: Pfizer Drug Safety Evaluation, Skokie, Illinois, USA. dan.g.morton@pfizer.com
authors.forename: Daniel
authors.initials: D
authors.lastname: Morton
authors.forename: Rani S
authors.initials: RS
authors.lastname: Sellers
authors.forename: Sylvia M
authors.initials: SM
authors.lastname: Furst
authors.forename: Kristen D
authors.initials: KD
authors.lastname: Hawley
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: May
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004334:Drug Administration Schedule / Q000662:veterinary*; D005260:Female; D051379:Mice; D008822:Mice, Transgenic; D009929:Organ Size / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage* / Q000633:toxicity*; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D023382:Toxicity Tests, Acute / Q000662:veterinary; D014867:Water / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D014867:Water; D012967:Sodium Dodecyl Sulfate