Heat shock and cold shock in Deinococcus radiodurans.
On the basis of acquired thermotolerance and cryotolerance, the optimal heat shock and cold shock temperatures have been determined for Deinococcus radiodurans. A heat shock at 42 degrees C maximized survival at the lethal temperature of 52 degrees C and a cold shock at 20 degrees C maximized survival after repeated freeze-thawing. Enhanced survival from heat shock was found to be strongly dependent on growth stage, with its greatest effect shortly after phase. Increased synthesis of a total of 67 proteins during heat shock and 42 proteins during cold shock were observed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and autoradiography. Eight of the most highly induced heat shock proteins shown by 2D PAGE were identified by MALDI-MS as Hsp20, GroEL, DnaK, SodA, Csp, Protease I, and two proteins of unknown function.
Airo
Alessandro
A
Department of Geological & Environmental Sciences, Stanford University, Stanford, CA, USA.
Chan
Suzanne L
SL
Martinez
Zak
Z
Platt
Manu O
MO
Trent
Jonathan D
JD
D001345:Autoradiography; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D003080:Cold Temperature; D034301:Deinococcus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism; D018869:Heat-Shock Response; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins