Function of the fibronectin-binding serum opacity factor of Streptococcus pyogenes in adherence to epithelial cells.
The serum opacity factor (SOF) of Streptococcus pyogenes is a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF in S. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherence of S. pyogenes to epithelial cells. SOF exclusively targets the 30-kDa N-terminal region of fibronectin. The interaction revealed association and dissociation constants 1 order of magnitude lower than those of other S. pyogenes fibronectin-binding proteins.
Oehmcke
Sonja
S
Department of Medical Microbiology and Hospital Hygiene, University Hospital Rostock, Schillingallee 70, 18055 Rostock, Germany.
Podbielski
Andreas
A
Kreikemeyer
Bernd
B
D001665:Binding Sites; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010447:Peptide Hydrolases / Q000276:immunology / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000378:metabolism*
D005353:Fibronectins; C021908:opacity factor; D010447:Peptide Hydrolases