Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry.
MALDI, while providing advantages such as the ability to do in-depth and repeated exploration of the sample, challenges the existing performance capabilities of Fourier transform mass spectrometry (FTMS). The challenge arises because MALDI-produced ions have high mass-to-charge ratios and uncertain kinetic-energy distributions. We demonstrate that a combination of a gated trapping event, a RF-only mode pressure focusing event, and an electrically compensated trap provides a compelling advantage in meeting these challenges. Removal of any of the above combination elements significantly degrades the detection performance of substance P from 850 K resolving power at 34.9 kHz and of melittin from 278 K resolving power at 16.5 kHz when using a 3-Tesla magnet-based spectrometer.
Gooden
Jonathon K
JK
Department of Chemistry, Washington University, St. Louis, Missouri, USA.
Rempel
Don L
DL
Gross
Michael L
ML
D013058:Mass Spectrometry / Q000379:methods*; D008555:Melitten / Q000032:analysis / Q000737:chemistry*; D011846:Radio Waves; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D019097:Spin Trapping / Q000295:instrumentation / Q000379:methods; D013373:Substance P / Q000032:analysis / Q000737:chemistry*
D008555:Melitten; D013373:Substance P