title: Proteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi.
abstract: Trypanosoma cruzi, the causative agent of Chagas disease, has evolved particular mechanisms of gene regulation. Gene expression is regulated firstly at post-transcriptional level. This feature makes proteomic methods a promising tool for studying adaptative changes in these parasites. In this work we generated a reproducible method for protein analysis by two-dimensional electrophoresis coupled to mass spectrometry, and a protein map for T. cruzi. Western-blot analysis supported the identity of some of the proteins. This work points to proteomic approach as a powerful tool to study differential expression, stress response or drug resistance in T. cruzi.
authors.affiliation: Laboratorio de Bioquímica Analítica, Instituto de Investigaciones Biológicas Clemente Estable/Facultad de Ciencias, Montevideo, Uruguay.
authors.forename: Adriana
authors.initials: A
authors.lastname: Parodi-Talice
authors.forename: Rosario
authors.initials: R
authors.lastname: Durán
authors.forename: Nicolás
authors.initials: N
authors.lastname: Arrambide
authors.forename: Victoria
authors.initials: V
authors.lastname: Prieto
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Piñeyro
authors.forename: Otto
authors.initials: O
authors.lastname: Pritsch
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cayota
authors.forename: Carlos
authors.initials: C
authors.lastname: Cerveñansky
authors.forename: Carlos
authors.initials: C
authors.lastname: Robello
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002453:Cell Cycle / Q000235:genetics; D014355:Chagas Disease / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D015870:Gene Expression / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D020543:Proteome; D015800:Protozoan Proteins; D014404:Tubulin