[Accelerated angiogenesis in artificial dermis with allogenic cultured dermal fibroblasts transplantation].
OBJECTIVE
To evaluate the effects of cryopreserved cultured allogenic dermal fibroblasts on angiogenesis and fibroplasia while artificial dermis grafting by spraying the cells on the graft bed.


METHODS
Full thickness skin defect was made on the back of Wistar rat, fibroblasts mixed into fibrin glue (fibroblast group) and same amount fibrin glue (control group) were sprayed separately between the wound bed and artificial dermis in cell density of 1.0 x 10(5) cells/cm2 before the artificial dermis was grafted. On day 5 after grafting, the graft and surrounding tissue were examined histologically for angiogenesis and fibroplasia in the dermis and wound bed with hematoxylin eosin stain, VEGF antibody stain, Masson's trichrome stain and India ink stain. Evans blue perfusion method was also used for detecting the angiogenesis quantitatively.


RESULTS
In the fibroblast group, the angiogenesis of graft bed was significantly accelerated on day 5 after grafting; the numbers of the newly formed capillaries were 9.64 +/- 2.36/HP in the fibroblast group and 3.88 +/- 1.62/HP in the control group (P < 0.05). And on day 10 after grafting the angiogenesis was accelerated not only in graft bed but also in the artificial dermis when compared with control group, the newly formed capillaries network was clearly observed in the artificial dermis. Otherwise, the synthesis of collagen was increased in the dermis on day 10 after grafting in the fibroblast group when compared with control group. The immunoreactivity of VEGF antibody in the fibroblast group also showed a stronger expression than that in control group on day 5 after grafting, the numbers of positive cells were 46.04 +/- 8.90/HP in the fibroblast group and 30.08 +/- 7.76/HP in the control group(P < 0.05).


CONCLUSIONS
Transplantation of cryopreserved dermal fibroblasts while artificial dermis grafting can accelerate the angiogenesis and fibroplasia in the artificial dermis and graft bed, thereby accelerate the formation of dermal-like tissue in the artificial dermis.
Chen
Xin
X
Department of Burns, Beijing Jishuitan Hospital, Beijing, 100035, P. R. China.
Soejima
Kazutaka
K
Nozaki
Motohiro
M
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology / Q000637:transplantation*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology; D051381:Rats; D012867:Skin / Q000293:injuries; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D013194:Staining and Labeling; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000502:physiology
D003094:Collagen