Development of a disposable potentiometric sensor for the near patient testing of plasma thiol concentrations.
The reaction of naphthoquinone with physiological thiols has been investigated as the basis of a NPT strategy for the measurement of the latter. Screen-printed carbon electrode assemblies provide an inexpensive and inherently disposable platform for the detection methodology. The key analytical parameters underpinning the selective and sensitive (0.4 uM-1 mM) determination of reduced thiol have been assessed, and the clinical efficacy of the approach has been demonstrated through its application to the analysis of human plasma. The results have been corroborated using standard techniques, and the routes through which the system can be adopted within mainstream biomedical environments are highlighted.
Gracheva
Svetlana
S
Chemistry, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, GU2 7XH, U.K.
Livingstone
Callum
C
Davis
James
J
D000368:Aged; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004566:Electrodes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D010865:Pilot Projects; D019095:Point-of-Care Systems; D011199:Potentiometry / Q000295:instrumentation* / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000097:blood*; D013499:Surface Properties; D013997:Time Factors
D013438:Sulfhydryl Compounds; D002244:Carbon