The proteome of chicken skeletal muscle: changes in soluble protein expression during growth in a layer strain.
The whole animal, and the pectoralis muscle in particular, grows at a greatly enhanced rate in chickens selected for meat production (broilers) when compared to those selected for egg production (layers). As part of an ongoing study to analyse muscle protein dynamics under conditions of rapid growth, we have embarked upon a preliminary characterisation of the proteome of layer chicken pectoralis muscle, at specified time-points from 1 to 27 days after hatching. Soluble extracts of muscle homogenates were separated by two-dimensional (2-D) gel electrophoresis and selected spots were analysed by in-gel tryptic digestion and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Of 90 spots, 51 gave mass spectra that matched to existing chicken proteins present in on-line databases, 12 matched equivalent proteins from non-avian species and 11 yielded good quality spectra but were unable to be matched against existing databases. For many of these proteins, growth over 27 days elicited dramatic changes in relative expression levels. Chicken skeletal muscle offers an excellent system for developmental proteomics.
Doherty
Mary K
MK
Protein Function Group, Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, UK.
McLean
Lynn
L
Hayter
Julia R
JR
Pratt
Julie M
JM
Robertson
Duncan H L
DH
El-Shafei
Abdel
A
Gaskell
Simon J
SJ
Beynon
Robert J
RJ
D000818:Animals; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010369:Pectoralis Muscles / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D010455:Peptides; D011506:Proteins; D020543:Proteome