title: Multiserotype protection of mice against pneumococcal colonization of the nasopharynx and middle ear by killed nonencapsulated cells given intranasally with a nontoxic adjuvant.
abstract: Intranasal challenge of C57BL/6 mice with Streptococcus pneumoniae serotypes 6B, 14, and 23F produced colonization of the middle ear and NP. Intranasal vaccination with ethanol-killed nonencapsulated cells with adjuvant protected both sites. Of four nontoxic adjuvants tested, the cholera toxin B subunit was most effective and least nonspecifically protective.
authors.affiliation: Division of Infectious Diseases, Children's Hospital, 300 Longwood Ave., Boston, MA 02115, USA. richard.malley@childrens.harvard.edu
authors.forename: Richard
authors.initials: R
authors.lastname: Malley
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Morse
authors.forename: Luciana C C
authors.initials: LC
authors.lastname: Leite
authors.forename: Ana Paula Mattos
authors.initials: AP
authors.lastname: Areas
authors.forename: Paulo Lee
authors.initials: PL
authors.lastname: Ho
authors.forename: Flavia S
authors.initials: FS
authors.lastname: Kubrusly
authors.forename: Igor C
authors.initials: IC
authors.lastname: Almeida
authors.forename: Porter
authors.initials: P
authors.lastname: Anderson
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D004427:Ear Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D004432:Ear, Middle / Q000276:immunology / Q000382:microbiology; D051379:Mice; D009302:Nasopharyngeal Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D011008:Pneumococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000276:immunology* / Q000494:pharmacology; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D022242:Pneumococcal Vaccines