Human histone deacetylase SIRT2 interacts with the homeobox transcription factor HOXA10.
Histone deacetylases are required for transcriptional repression in eukaryotes. Saccharomyces cerevisiae has several histone deacetylases, of which ySir2p is the most conserved throughout evolution. Currently, there is no report on the interacting protein partner of a human Sir2 homolog, SIRT2. Here we show for the first time that SIRT2 interacts with the homeobox transcription factor, HOXA10, which was identified in a two-hybrid screen. Interactions were confirmed by co-immunoprecipitation from in vitro translations as well as in human cell-free extracts. Taken together with mouse knockout studies, our results raise the intriguing possibility that SIRT2 plays a role in mammalian development.
Bae
Nancy S
NS
Laboratory of Molecular and Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. NSB@stowers-institute.org
Swanson
Mark J
MJ
Vassilev
Alex
A
Howard
Bruce H
BH
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D006801:Humans; D051379:Mice; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D011993:Recombinant Fusion Proteins; C069634:HOXA10 protein, human; C474199:SIRT2 protein, human; D056565:Sirtuin 2; D037761:Sirtuins