title: Qualitative and quantitative liquid chromatographic analysis methods for the determination of the effects of feed supplements on hen egg yolk phospholipids.
abstract: Quantitative and qualitative high-performance liquid chromatographic methods were utilized to separate phospholipid classes. After qualitative separation, the fatty acid moieties of each separated phospholipid class were determined using a gas chromatographic method. On the basis of these analyses, the effect of supplemented feeds on hen egg yolk lipids can be evaluated. The supplemented feeds contained 1-5% of vegetable-based or fish oils. The phospholipid content and composition were the same in all feeding groups, the proportions of phosphatidylcholines, phosphatidylethanolamines, and sphingomyelins being 70, 28, and 3%, respectively. In each feeding group, the fatty acid profiles of phosphatidylcholines and sphingomyelins were similar to each other and different from that of phosphatidylethanolamines. The supplemented feeds had a statistically significant (p < 0.05) effect on the fatty acid composition of phosphatidylcholines. The supplements decreased the proportion of saturated fatty acids in total fat, but this effect was not found in phospholipids.
authors.affiliation: Department Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Kivini
authors.forename: Eila P
authors.initials: EP
authors.lastname: Järvenpää
authors.forename: Heikki
authors.initials: H
authors.lastname: Aro
authors.forename: Rainer
authors.initials: R
authors.lastname: Huopalahti
authors.forename: Eeva-Liisa
authors.initials: EL
authors.lastname: Ryhänen
mesh_terms: D000821:Animal Feed; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements; D004530:Egg Yolk / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D005227:Fatty Acids; D010743:Phospholipids