Aptamer selection for the inhibition of cell adhesion with fibronectin as target.
An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.
Ogawa
Atsushi
A
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan.
Tomita
Naotoshi
N
Kikuchi
Naoko
N
Sando
Shinsuke
S
Aoyama
Yasuhiro
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D005353:Fibronectins / Q000037:antagonists & inhibitors / Q000502:physiology*; D007668:Kidney; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000494:pharmacology*; D011487:Protein Conformation
D005353:Fibronectins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid