title: The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in DeltapH formation across peroxisomal membranes.
abstract: The yeast peroxisomal adenine nucleotide carrier, Ant1p, was shown to catalyse unidirectional transport in addition to exchange of substrates. In both transport modes, proton movement occurs. Nucleotide hetero-exchange is H+-compensated and electroneutral. Furthermore, microscopic fluorescence imaging of a pH-sensitive green fluorescent protein targeted to peroxisomes shows that Ant1p is involved in the formation of a DeltapH across the peroxisomal membrane, acidic inside.
authors.affiliation: Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, 70125 Bari, Italy.
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Lasorsa
authors.forename: Pasquale
authors.initials: P
authors.lastname: Scarcia
authors.forename: Ralf
authors.initials: R
authors.lastname: Erdmann
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Palmieri
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Rottensteiner
authors.forename: Luigi
authors.initials: L
authors.lastname: Palmieri
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D001693:Biological Transport, Active / Q000502:physiology; D003600:Cytosol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000737:chemistry; D033704:Nucleotide Transport Proteins / Q000378:metabolism / Q000502:physiology*; D020675:Peroxisomes / Q000737:chemistry* / Q000378:metabolism; D018892:Proton-Motive Force / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: C430871:ANT1 protein, S cerevisiae; D000227:Adenine Nucleotides; D033704:Nucleotide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins