Fate of donor hematopoietic cells in demyelinating mutant mouse, twitcher, following transplantation of GFP+ bone marrow cells.
The twitcher mouse is an authentic murine model of a genetic demyelinating disease globoid cell leukodystrophy. Allogeneic bone marrow transplantation (BMT) in twitcher mice resulted in the clinicopathological improvement. Thus, using green fluorescent protein (GFP) transgenic mice as the donor, we investigated the behavior and fate of the donor cells and the possibility of transdifferentiation of the donor cells into neuroglial cells in the chimeric twitcher mice. GFP(+) cells were found throughout the brain, most conspicuous in the areas of demyelination. The donor GFP(+) cells expressed RCA-1, a marker for microglia/macrophages but were never exceed 70% of the entire population of the RCA-1(+) microglia/macrophages. There was no convincing evidence that GFP(+) donor cells expressed markers for neurons, astrocytes, or oligodendrocytes. We concluded BMT is therapeutic for this model. However, this effect is not mediated by donor cells transdifferentiation in the brain.
Yagi
Takashi
T
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
McMahon
Eileen J
EJ
Takikita
Shoichi
S
Mohri
Ikuko
I
Matsushima
Glenn K
GK
Suzuki
Kinuko
K
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods* / Q000473:pathology; D003711:Demyelinating Diseases / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D005260:Female; D005838:Genotype; D049452:Green Fluorescent Proteins; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000166:cytology; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000737:chemistry / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D008822:Mice, Transgenic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins