title: Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures.
abstract: In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and gamma delta T-cells were analyzed by flow cytometry, and the production of interleukin-1 beta and prostaglandin E(2) was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and gamma delta T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.
authors.affiliation: Department of Periodontology and Immunology Lab, Faculty of Dentistry, Chulalongkorn University, Henry Dunant Rd., Bangkok 10330, Thailand. mrangsin@chula.ac.th
authors.forename: R
authors.initials: R
authors.lastname: Mahanonda
authors.forename: N
authors.initials: N
authors.lastname: Sa-Ard-Iam
authors.forename: O
authors.initials: O
authors.lastname: Charatkulangkun
authors.forename: A
authors.initials: A
authors.lastname: Promsudthi
authors.forename: R E
authors.initials: RE
authors.lastname: Schifferle
authors.forename: K
authors.initials: K
authors.lastname: Yongvanichit
authors.forename: S
authors.initials: S
authors.lastname: Pichyangkul
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D008262:Macrophage Activation / Q000276:immunology*; D008297:Male; D016555:Matched-Pair Analysis; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010518:Periodontitis / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D012016:Reference Values; D012720:Severity of Illness Index; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D008070:Lipopolysaccharides