title: Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus.
abstract: A Foxl2 cDNA was cloned from the Nile tilapia ovary by RT-PCR and subsequent RACE. Alignment of known Foxl2 sequences from vertebrates confirmed the conservation of the Foxl2 open reading frame and protein sequences, especially the forkhead domain and C-terminal region, while some homopolymeric runs of amino acids are found only in mammals but not in non-mammalian vertebrates. RT-PCR revealed that Foxl2 is expressed in the tilapia brain (B), pituitary (P), gill, and gonads (G), with the highest level of expression in the ovary, reflecting the involvement of Foxl2 in B-P-G axis. Northern blotting and in situ hybridization also revealed an evident sexual dimorphic expression pattern in the gonads. Foxl2 mRNA was mainly detected in the granulosa cells surrounding the oocytes. The ovarian expression of Foxl2 in tilapia begins early during the differentiation of the gonads and persists until adulthood, implying the involvement of Foxl2 in fish gonad differentiation and the maintenance of ovarian function.
authors.affiliation: Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: Deshou
authors.initials: D
authors.lastname: Wang
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Linyan
authors.initials: L
authors.lastname: Zhou
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nagahama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D023681:Cichlids / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D006066:Gonads / Q000196:embryology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors