[Expression of P62 in gallbladder carcinoma tissues and its correlation with angiogenesis].
OBJECTIVE
To detect the expressions of P62 and bFGF in human gallbladder carcinoma tissues and microvessel density(MVD) inside tumor, explore their correlation with each other, and their relationship with tumor's clinical pathological properties.


METHODS
The expressions of P62 and bFGF in 41 cases of human gallbladder carcinoma tissues and 22 cases of chronic cholecystitis tissues were detected by SP immunohistochemical staining. The MVD was examined by SP immunohistochemical staining with anti CD34 mAb.


RESULTS
The positive rates of P62 and bFGF in 41 cases of gallbladder carcinoma tissues (63.4% and 75.6%, respectively) were significantly higher than those in 22 cases of chronic cholecystitis tissues(P<0.01). The expression of P62 was associated with lymph node metastasis(P<0.05), but had no relation to tumor's histological grading and clinical stage. The expression of bFGF was correlated with tumor's histological grading and clinical stage, but not with lymph node metastasis. There was a positive correlation between P62 and bFGF expression rates(r = 0.512, P<0.01). Both proteins had no relation with patient's age, sex, tumor's type or cholelithiasis. MVD in the gallbladder carcinoma tissues was significant higher than that in the chronic cholecystitis tissues(P<0.01). The expression rates of P62 and bFGF were all correlated with MVD in gallbladder carcinoma tissues(P<0.05 and P<0.01), respectively. MVD in the gallbladder carcinoma tissues was associated with clinical stages and lymph node metastasis status (P<0.05, and P<0.01), respectively. but had no relation with patient's age, sex, tumor's type, histological grading and cholelithiasis.


CONCLUSIONS
Expressions of P62 and bFGF were closely associated with angiogenesis in gallbladder carcinoma tissues, which may play a pivotal role in genesis and progression in gallbladder carcinoma. Expressions of P62 and bFGF may be helpful for diagnosis and therapy of gallbladder carcinoma.
Wang
Jiang
J
Department of General Surgery, Urumqi General Hospital, Urumqi 830002, China. wangjiangweiran@hotmail.com
Dou
Ke-feng
KF
Zhao
Rong
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005706:Gallbladder Neoplasms / Q000098:blood supply / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
D018952:Antigens, CD34; C118585:P62 protein, human; D016601:RNA-Binding Proteins; D016222:Fibroblast Growth Factor 2