Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma.
OBJECTIVE
To analyze loss of heterozygosity (LOH) and homozygous deletion on p53 gene (exon2-3, 4 and 11), chromosome 10q22-10q23 and 22q11.2 -22q12.1 in human hepatocellular carcinoma (HCC).


METHODS
PCR and PCR-based microsatellite polymorphism analysis techniques were used.


RESULTS
LOH was observed at D10S579 (10q22-10q23) in 4 of 20 tumors (20%), at D22S421 (22q11.2-22q12.1) in 3 of 20(15%), at TP53.A (p53 gene exon 2-3) in 4 of 20(20%), at TP53.B (p53 gene exon 4) in 6 of 20(30%), and at TP53.G (p53 gene exon 11) in 0 of 20(0%). Homozygous deletion was detected at 10q22-10q23(8/20; 40%), 22q11.2-22q12.1(8/20; 40%), p53 gene exon 2-3(0/20;0%), p53 gene exon 4(6/20; 30%), and p53 gene exon 11(2/20; 10%).


CONCLUSIONS
There might be unidentified tumor suppressor genes on chromosome 10q22-10q23 and 22q11.2-22q12.1 that contribute to the pathogenesis and development of HCC.
Zhu
Guang-Neng
GN
Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China.
Zuo
Li
L
Zhou
Qing
Q
Zhang
Su-Mei
SM
Zhu
Hua-Qing
HQ
Gui
Shu-Yu
SY
Wang
Yuan
Y
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D005091:Exons; D017353:Gene Deletion; D006720:Homozygote; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53