Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer.
OBJECTIVE
To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer.


METHODS
Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in 45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper.


RESULTS
The LOH frequencies in gastric cancer at all 14 markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23(6cM) with 32% LOH frequency.


CONCLUSIONS
LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions.
Yu
Jing-Cui
JC
Department of Medical Genetics, China Medical University, Shenyang 110001, China.
Sun
Kai-Lai
KL
Liu
Buo
B
Fu
Song-Bin
SB
D000483:Alleles; D002887:Chromosomes, Human, Pair 18; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D013274:Stomach Neoplasms / Q000235:genetics*
D005819:Genetic Markers