title: Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.
abstract: Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.
authors.affiliation: Max Planck Institute for Medical Research, Heidelberg, Germany. mhasan@mpimf-heidelberg.mpg.de
authors.forename: Mazahir T
authors.initials: MT
authors.lastname: Hasan
authors.forename: Rainer W
authors.initials: RW
authors.lastname: Friedrich
authors.forename: Thomas
authors.initials: T
authors.lastname: Euler
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Larkum
authors.forename: Günter
authors.initials: G
authors.lastname: Giese
authors.forename: Matthias
authors.initials: M
authors.lastname: Both
authors.forename: Jens
authors.initials: J
authors.lastname: Duebel
authors.forename: Jack
authors.initials: J
authors.lastname: Waters
authors.forename: Hermann
authors.initials: H
authors.lastname: Bujard
authors.forename: Oliver
authors.initials: O
authors.lastname: Griesbeck
authors.forename: Roger Y
authors.initials: RY
authors.lastname: Tsien
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Winfried
authors.initials: W
authors.lastname: Denk
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D004318:Doxycycline; D036681:Fluorescence Recovery After Photobleaching; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009830:Olfactory Bulb / Q000502:physiology*; D010775:Photic Stimulation; D012160:Retina / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
chemical_list: D015220:Calcium Channels; D008164:Luminescent Proteins; D004318:Doxycycline; D002118:Calcium