The most common chromosome aberration detected by high-resolution comparative genomic hybridization in vulvar intraepithelial neoplasia is not seen in vulvar squamous cell carcinoma.
We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. All samples were also human papilloma virus (HPV)-genotyped. Gain of chromosome 1, the aberration most often seen in VIN III (67%), was not seen in HPV-positive or -negative VSCCs (0%). Both VIN III and VSCC frequently showed gain of 3q (56 and 70%, respectively). The VIN III samples often demonstrated gain of 20q (56%) and 20p (44%), and the VSCC samples gain of 8q (60%), loss of 3p (50%), and 8p (40%). None of the four most frequent changes in the VSCC samples occurred exclusively in the HPV-positive or -negative samples. As expected, we did not find any cytogenetic changes in condylomas and nearly any changes in VIN I-II.
Bryndorf
T
T
Fertility Clinic, Rigshospitalet, Denmark. thue.bryndorf@dadlnet.dk
Kirchhoff
M
M
Larsen
J
J
Andreasson
B
B
Bjerregaard
B
B
Westh
H
H
Rose
H
H
Lundsteen
C
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002278:Carcinoma in Situ / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D003218:Condylomata Acuminata / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000235:genetics; D014314:Trisomy; D014846:Vulvar Neoplasms / Q000235:genetics* / Q000821:virology