Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia enterocolitica.
YscC is the integral outer membrane component of the type III protein secretion machinery of Yersinia enterocolitica and belongs to the family of secretins. This group of proteins forms stable ring-like oligomers in the outer membrane, which are thought to function as transport channels for macromolecules. The YscC oligomer was purified after solubilization from the membrane with a nonionic detergent. Sodium dodecyl sulfate did not dissociate the oligomer, but it caused a change in electrophoretic mobility and an increase in protease susceptibility, indicating partial denaturation of the subunits within the oligomer. The mass of the homo-oligomer, as determined by scanning transmission electron microscopy, was approximately 1 MDa. Analysis of the angular power spectrum from averaged top views of negatively stained YscC oligomers revealed a 13-fold angular order, suggesting that the oligomer consists of 13 subunits. Reconstituted in planar lipid bilayers, the YscC oligomer displayed a constant voltage-independent conductance of approximately 3 nS, thus forming a stable pore. However, in vivo, the expression of YscC did not lead to an increased permeability of the outer membrane. Electron microscopy revealed that the YscC oligomer is composed of three domains, two stacked rings attached to a conical domain. This structure is consistent with the notion that the secretin forms the upper part of the basal body of the needle structure of the type III secreton.
Burghout
Peter
P
Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
van Boxtel
Ria
R
Van Gelder
Patrick
P
Ringler
Philippe
P
Müller
Shirley A
SA
Tommassen
Jan
J
Koster
Margot
M
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D001692:Biological Transport; D002463:Cell Membrane Permeability; D004553:Electric Conductivity; D004586:Electrophoresis; D008051:Lipid Bilayers; D017348:Microscopy, Electron, Scanning Transmission; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D018272:Porins / Q000737:chemistry / Q000502:physiology; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D015008:Yersinia enterocolitica / Q000472:pathogenicity*
D001425:Bacterial Outer Membrane Proteins; D008051:Lipid Bilayers; D018272:Porins; D021122:Protein Subunits; D012967:Sodium Dodecyl Sulfate; D010447:Peptide Hydrolases