Contribution of bone-marrow-derived cells to choroidal neovascularization.
We investigated the involvement of bone-marrow derived cells to experimental choroidal neovascularization (CNV) in mice, whose bone marrow was reconstituted by either unfractionated bone-marrow cells or Lin-c(-)Kit(+)Sca-1+ enriched presumable hematopoietic stem cells from the green fluorescent protein (GFP) transgeneic mice. Immunohistochemical analysis demonstrated the presence of GFP-positive cells in the CNV lesion after unfractionated bone-marrow transplantation, as well as Lin-c(-)Kit(+)Sca-1+ cell transplantation. Some of the GFP-expressing cells also expressed CD-31 and PanEC antigen, markers of vascular endothelial cells. Our results suggest that bone-marrow derived cells may contribute endothelial cells in CNV.
Takahashi
Hidenori
H
Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Yanagi
Yasuo
Y
Tamaki
Yasuhiro
Y
Muranaka
Kimimasa
K
Usui
Tomohiko
T
Sata
Masataka
M
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D020256:Choroidal Neovascularization / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins