title: Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
abstract: Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.
authors.affiliation: Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. tera@obara.elec.keio.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Terakawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Ogura
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sato
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Wakisaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ashida
authors.forename: Maki
authors.initials: M
authors.lastname: Uenoyama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Obara
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects*; D002463:Cell Membrane Permeability / Q000528:radiation effects*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins; D007834:Lasers; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics*; D013314:Stress, Mechanical; D013696:Temperature; D014162:Transfection / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins