title: Anaerobiosis and low specific growth rates enhance hemolysin BL production by Bacillus cereus F4430/73.
abstract: Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1-0.2 h(-1)) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h(-1), the presence of 90 mM glucose resulted in inhibition of HBL production. Glucose was found to repress HBL induction at the mRNA level, indicating the potential involvement of catabolite repression in the regulation of HBL. Based on these data, it is suggested that growth rate could be an effector of catabolite regulation of HBL.
authors.affiliation: UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, INRA/Université d'Avignon, INRA Domaine St Paul, 84914, Avignon cedex 9, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Duport
authors.forename: Séverine
authors.initials: S
authors.lastname: Thomassin
authors.forename: Gérald
authors.initials: G
authors.lastname: Bourel
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt
mesh_terms: D000693:Anaerobiosis / Q000502:physiology*; D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006460:Hemolysin Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C083961:hemolysin BL protein, Bacillus; D005947:Glucose