Effect of medium osmotic potential on callus induction and shoot regeneration in flax anther culture.
Development of an efficient and cost-effective doubled haploid production system in flax (Linum usitatissimum L.) is the prerequisite for the application of doubled haploid technology in a practical breeding program. Pre-culture of anthers on a medium containing 15% sucrose for 2-7 days before transfer to the same medium containing 6% sucrose for a total of 28 days culture period significantly increased shoot regeneration for all four genotypes evaluated. Moreover, pre-culture of anthers on medium containing 15% sucrose for 2-7 days was sufficient to dramatically reduce the frequency of shoot regeneration from somatic tissues and thereby to increase the frequency of microspore-derived plants in flax anther culture. Furthermore, replacing 15% sucrose with 6% sucrose and 9% polyethylene glycol (PEG), or 3% sucrose and 12% PEG, in pre-culture medium did not significantly affect callus induction and shoot regeneration. The results indicate that sucrose may act as carbon/energy source as well as an osmotic regulator in flax anther culture. Sucrose as an osmotic regulator may be replaced by a non-metabolizable osmoticum: PEG. The implication of this study in flax anther culture and breeding is discussed.
Chen
Yurong
Y
Linum Research Program, Agricore United, Unit 100-101, Route 100, Morden, MB, R6M 1Y5, Canada. ychen@agricoreunited.com
Dribnenki
Paul
P
D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods; D004734:Energy Metabolism; D019597:Flax / Q000254:growth & development* / Q000378:metabolism; D035264:Flowers / Q000254:growth & development* / Q000378:metabolism; D009997:Osmotic Pressure; D018520:Plant Shoots / Q000254:growth & development* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology
D003470:Culture Media; D011092:Polyethylene Glycols; D013395:Sucrose