title: Detection of DNA hybridization on a liposome surface using ultrasound velocimetry and turbidimetry methods.
abstract: 19-mer oligonucleotides with oleylamine tethered at 3' and 5' terminal, respectively, were incorporated into unilamellar liposomes of dioleoylphosphatidylcholine (DOPC). Addition of complementary nucleotide resulted in hybridization with oligonucleotides located on different liposomes and caused liposome aggregation. Significant changes of sound velocimetry and turbidity were readily observed at 10 nM concentration of the complementary chain.
authors.affiliation: Department of Biophysics and Chemical Physics, Comenius University, 842 48 Bratislava, Slovakia. Hianik@fmph.uniba.sk
authors.forename: Tibor
authors.initials: T
authors.lastname: Hianik
authors.forename: Peter
authors.initials: P
authors.lastname: Rybar
authors.forename: Sergej Yu
authors.initials: SY
authors.lastname: Andreev
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Oretskaya
authors.forename: Pankaj
authors.initials: P
authors.lastname: Vadgama
mesh_terms: D001483:Base Sequence; D011088:DNA Ligases; D004279:DNA, Viral / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D004279:DNA, Viral; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D011088:DNA Ligases