title: [Cloning of hIL-1Ra gene and its expression in E.coli].
abstract: OBJECTIVE
  To clone human IL-1Ra gene and express it in E.coli.
  
  
  METHODS
  Human IL-1Ra cDNA was obtained by RT-PCR with the the total RNA extracted from human peripheral blood leucocytes as template. The cDNA was cloned into pBV220 vector and expressed. The expressed product was renatured and purified. Separation, purification and bioactivity analysis of the expressed products were performed.
  
  
  RESULTS
  IL-1Ra gene was successfully expressed in E.coli and the expression level reached to about 40% of total bacteria protein. The purity of the final product was over 98%. The product could obviously suppress the secretion of IL-2 by EL-4 cells stimulated with IL-1beta.
  
  
  CONCLUSIONS
  The expression of hIL-1Ra gene with bioactivity in E.coli lays experimental foundation for further development and utilisation.
authors.affiliation: Insititute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Shao-hong
authors.initials: SH
authors.lastname: Chang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Gong
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D053590:Interleukin 1 Receptor Antagonist Protein; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D020673:Protein Renaturation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007376:Interleukin-2; D011994:Recombinant Proteins; D012795:Sialoglycoproteins