Phospholipase C delta-type consists of three isozymes: bovine PLCdelta2 is a homologue of human/mouse PLCdelta4.
To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, beta-, gamma-, delta-, epsilon-, and zeta-type. PLCdelta-type is reported to be composed of four isozymes, PLCdelta1-delta4. Here we report that a screening for mouse PLCdelta2 from a BAC library with primers that amplify a specific region of bovine PLCdelta2 resulted in isolation of one clone containing the mouse PLCdelta4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCdelta2 and PLCdelta4 in the mouse and human genomes, indicating that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4. However, PLCdelta2 Western blot analysis with a widely used commercial anti-PLCdelta2 antibody showed an expression pattern distinct from that of PLCdelta4 in wild-type mice. In addition, an 80-kDa band, which was recognized by antibody against PLCdelta2, was smaller than an 85-kDa band detected by anti-PLCdelta4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCdelta4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCdelta2 antibody contained no PLC activity. From these data, we conclude that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4, and that three isozymes (delta1, delta3, and delta4) exist in the PLCdelta family.
Irino
Yasuhiro
Y
Laboratory of Genome and Biosignal, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Tokyo, Japan.
Cho
Hiroyuki
H
Nakamura
Yoshikazu
Y
Nakahara
Masamichi
M
Furutani
Masahiro
M
Suh
Pann-Ghill
PG
Takenawa
Tadaomi
T
Fukami
Kiyoko
K
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D054803:Phospholipase C delta; D010738:Type C Phospholipases / Q000378:metabolism*
D017931:DNA Primers; D007527:Isoenzymes; D010738:Type C Phospholipases; C518024:PLCD1 protein, human; C518028:PLCD3 protein, human; C518030:PLCD4 protein, human; D054803:Phospholipase C delta; C518025:Plcd1 protein, mouse; C518027:Plcd2 protein, mouse; C518031:Plcd4 protein, mouse