[Localization of ATPase activity, respiration and ultrastructure of wheat root cells with modulated ion conductivity of plasma membrane].
Changes in the localization of ATPase activity, respiration and ultrastructure of wheat root cells with modulated ion conductivity of plasma membrane were studied. A 2 h treatment of excised root with valinomycin (20 microM), N,N-dicyclohexylcarbodiimid (100 microM), gramicidin S (20 microM) and chlorpromazine (100 microM) caused an increased loss of potassium by cells, a decreased respiration and changes in the localization of ATPase activity and in cell ultrastructure. Differences in the observed changes may be conditioned by different mechanisms of action of the membrane active compounds used. It is concluded that changes in the localization of ATPase activity and ultrastructure may indicate some early specific responses of root cells, whereas the increase in the ion conductivity and decrease in respiration under disruption of ion homeostasis caused by membrane active compounds indicate unspecific responses of cells.
Chernysheva
F A
FA
Institute of Biochemistry and Biophysics, Research Center RAS, Kazan.
Alekseeva
V Ia
VIa
Polygalova
O O
OO
Gordon
L Kh
LKh
D000251:Adenosine Triphosphatases / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D019069:Cell Respiration / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D006096:Gramicidin / Q000494:pharmacology; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D010101:Oxygen Consumption; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014634:Valinomycin / Q000494:pharmacology
D007473:Ion Channels; D007476:Ionophores; D006096:Gramicidin; D014634:Valinomycin; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases; D011188:Potassium; D002746:Chlorpromazine