Temperature acclimation modifies Na+ current in fish cardiac myocytes.
The present study was designed to test the hypothesis that temperature acclimation modifies sarcolemmal Na+ current (INa) of the fish cardiac myocytes differently depending on the animal's lifestyle in the cold. Two eurythermal fish species with different physiological strategies for surviving in the cold, a cold-dormant crucian carp (Carassius carassius L.) and a cold-active rainbow trout (Oncorhynchus mykiss), were used in acclimation experiments. The INa of carp and trout were also compared with INa of a cold stenothermal burbot (Lota lota). In accordance with the hypothesis, cold-acclimation decreased the density of INa in crucian carp and increased it in rainbow trout, suggesting depression of impulse conduction in cold-acclimated carp and positive compensation of impulse propagation in cold-acclimated trout. The steady-state activation curve of trout INa was shifted by 6 mV to more negative voltages by cold acclimation, which probably lowers the stimulus threshold for action potentials and further improves cardiac excitability in the cold. In burbot myocytes, the INa density was high and the position of the steady-state activation curve on the voltage axis was even more negative than in trout or carp myocytes, suggesting that the burbot INa is adapted to maintain high excitability and conductivity in the cold. The INa of the burbot heart differed from those of carp and trout in causing four times larger charge influx per excitation, which suggests that INa may also have a significant role in cardiac excitation-contraction coupling of the burbot heart. In summary, INa of fish cardiac myocytes shows thermal plasticity that is different in several respects in cold-dormant and cold-active species and thus has a physiologically meaningful role in supporting the variable life styles and habitat conditions of each species.
Haverinen
Jaakko
J
University of Joensuu, Department of Biology, PO Box 111, 80101 Joensuu, Finland.
Vornanen
Matti
M
D000064:Acclimatization / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002347:Carps / Q000378:metabolism / Q000502:physiology*; D005387:Finland; D017136:Ion Transport; D007700:Kinetics; D032383:Myocytes, Cardiac / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012964:Sodium / Q000378:metabolism*; D013696:Temperature
D012964:Sodium