Crystallization and X-ray diffraction analysis of insect-cell-derived IL-22.
Interleukin-22 is a potent mediator of cellular inflammatory responses. Crystals of interleukin-22 expressed in Drosophila melanogaster S2 cells (IL-22Dm) have been grown from polyethylene glycol solutions. To obtain crystals suitable for X-ray diffraction analysis required the separation of different IL-22Dm glycosylation variants and the inclusion of the detergent cetyltrimethylammonium bromide (CTAB) in the crystallization experiments. The crystals belong to space group P2(1), with unit-cell parameters a = 64.88, b = 62.23, c = 139.524 A, beta = 91.35 degrees, and diffract X-rays to 2.6 A resolution. The crystallographic asymmetric unit contains six IL-22Dm molecules, corresponding to a solvent content of approximately 49%.
Xu
Ting
T
Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 35294, USA.
Logsdon
Naomi J
NJ
Walter
Mark R
MR
D000818:Animals; D002460:Cell Line; D003460:Crystallization; D018360:Crystallography, X-Ray; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics*; D006031:Glycosylation; D007378:Interleukins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000097043:Interleukin-22
D007378:Interleukins; D000097043:Interleukin-22