Contribution of antioxidants to preventive effect of mesna in cyclophosphamide-induced hemorrhagic cystitis in rats.
OBJECTIVE
The aim of this study was to evaluate whether combination of antioxidants and mesna may prevent cystitis induced by cyclophosphamide better than mesna alone.


METHODS
A total of 46 male Sprague-Dawley rats were divided into six groups. Five groups received single dose of cyclophosphamide (CP, 100 mg/kg) intraperitoneally with the same time intervals: group 2 received CP only, group 3 received mesna (21.5 mg/kg for three times), group 4 beta-carotene (20 mg/kg for two times) and mesna, group 5 received alpha-tocopherol (20 mg/kg for two times) and mesna, and group 6 received melatonin (5 mg/kg for two times) and mesna on the day of CP injection. Group 1 served as control.


RESULTS
CP injection resulted in severe cystitis. Mesna has showed meaningful but not full protection against CP toxicity. Although beta-carotene did not show any additional beneficial effect when combined with mesna, alpha-tocopherol and especially melatonin with mesna resulted full protection that the pathologist, blinded to the slides, could not differ from sham control.


CONCLUSIONS
Oxidants may be important in the pathogenesis of CP-induced cystitis. Melatonin and alpha-tocopherol may help to ameliorate bladder damage along with other drugs such as mesna and diuretics.
Yildirim
Ibrahim
I
Department of Urology, Gulhane Military Medical Academy, Ankara, Turkey.
Korkmaz
Ahmet
A
Oter
Sukru
S
Ozcan
Ayhan
A
Oztas
Emin
E
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D003520:Cyclophosphamide / Q000633:toxicity*; D003556:Cystitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D008297:Male; D015080:Mesna / Q000037:antagonists & inhibitors* / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000473:pathology
D000975:Antioxidants; D020011:Protective Agents; D003520:Cyclophosphamide; D015080:Mesna