The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides.
The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.
Fittkau
M H
MH
Cardiovascular Research Unit, Christian Barnard Department of Cardiothoracic Surgery, Cape Heart Centre, University of Cape Town, Anzio Road, 7925 Observatory, Cape Town, South Africa.
Zilla
P
P
Bezuidenhout
D
D
Lutolf
M P
MP
Human
P
P
Hubbell
J A
JA
Davies
N
N
D000327:Adsorption; D000971:Antineoplastic Combined Chemotherapy Protocols; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*
D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid