title: Effects of perinatal exposure to low doses of PCB 153 and PCB 126 on lymphocyte proliferation and hematology in goat kids.
abstract: Pregnant does (10 goats/group) were dosed orally with either PCB 153 or PCB 126 dissolved in corn oil or only corn oil (control group) from day 60 of gestation until delivery. Effects on in vitro mitogen-induced lymphocyte proliferation and blood cell counts in their goat kids exposed to low levels of PCB 153 and PCB 126 during gestation and lactation were assessed. The concentrations of PCB 153 and PCB 126 in adipose tissue in the goat kids 9 mo postpartum were 5800 ng/g (fat weight) and 0.49 ng/g (fat weight), respectively. Kids exposed to PCB 153 had a significantly higher number of white blood cells, neutrophils, and lymphocytes at 2 wk of age compared to controls. In the kids exposed to PCB 126 there was a significantly lower concentration of monocytes at 2, 4, and 8 wk of age. The mean lymphocyte response to phytohemagglutinin (PHA) and to concanavalin A (Con A) was significant lower in the PCB 153 compared to the control group at wk 2, 4, and 8 postnatally. The results of the present study support previous reports on immunotoxic effects of PCB exposure in animals. However, this is the first report to demonstrate immunotoxicity in animals by using low doses of PCB 153. The difference in results between PCB 126 and PCB 153 treatment groups may strengthen the hypothesis that PCBs mediate immunotoxic effects through both AhR-dependent and -independent mechanisms.
authors.affiliation: Department of Reproduction and Forensic Medicine, Norwegian School of Veterinary Science, Oslo, Norway.
authors.forename: Jan
authors.initials: J
authors.lastname: Lyche
authors.forename: Hans
authors.initials: H
authors.lastname: Larsen
authors.forename: Janneche Utne
authors.initials: JU
authors.lastname: Skaare
authors.forename: Aage
authors.initials: A
authors.lastname: Tverdal
authors.forename: Ellen
authors.initials: E
authors.lastname: Dahl
authors.forename: Grethe
authors.initials: G
authors.lastname: Johansen
authors.forename: Erik
authors.initials: E
authors.lastname: Ropstad
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D001772:Blood Cell Count; D004305:Dose-Response Relationship, Drug; D005260:Female; D006041:Goats; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000145:classification / Q000187:drug effects; D018811:Maternal Exposure; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D011247:Pregnancy
chemical_list: D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl