Molecular investigation of the stromal cell-derived factor-1 chemokine in lymphoid leukemia and lymphoma patients from Brazil.
The stromal cell-derived factor-1 (SDF-1) gene contains a common polymorphism, termed SDF1-3'A, in an evolutionarily conserved segment of the 3'-untranslated region (3'-UTR). We compared SDF-1 genotypes in patients diagnosed with lymphoid leukemias and lymphomas. Since the SDF1-3'A variant deletes the MspI restriction site, PCR-restriction fragment length polymorphism (RFLP) analysis was used for identification of genotypes. We identified the heterozygous genotype (3'A/wt) in 38.8% (24/62) of lymphoma patients and in 26.2% (11/42) of lymphoid leukemias. The percentage of 3'A carriers was significantly higher in lymphomas (43.5%) than in lymphoid leukemias (26.2%; P < 0.05). Our study indicates that lymphoma patients from Brazil are more likely to carry the 3'A gene than patients with lymphoid leukemias, suggesting that this polymorphism may be a differential determinant of lymphomas and lymphoid leukemia.
de Oliveira Cavassin
Gabriela Gonçalves
GG
Department of Pathological Sciences, Londrina State University, Londrina, PR, Brazil.
De Lucca
Fernando Luiz
FL
Delgado André
Nayara
N
Covas
Dimas Tadeu
DT
Pelegrinelli Fungaro
Maria Helena
MH
Voltarelli
Júlio César
JC
Watanabe
Maria Angelica Ehara
MA
D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D002648:Child; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006801:Humans; D007945:Leukemia, Lymphoid / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D008223:Lymphoma / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D008297:Male; D017720:Molecular Epidemiology; D011110:Polymorphism, Genetic
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC