title: Comparison of two mononuclear cell program settings on two apheresis devices intended to collect high yields of CD14+ and CD3+ cells.
abstract: BACKGROUND
  In cancer and transplantation therapy apheresis devices and software of optimum standards are required for the collection of high cell yields with high purity of the desired cell fraction.
  
  
  METHODS
  In a paired study, 15 healthy blood donors underwent four 10-L leukapheresis procedures (197 +/- 33 min) with an inlet blood flow rate of 60 mL per minute by use of two different MNC program settings of the COBE Spectra (Gambro BCT) and the AS.TEC 204 (Fresenius Hemocare) cell separators.
  
  
  RESULTS
  Use of the standard MNC program of both apheresis devices resulted in significantly higher (p < 0.01) collection efficiencies of CD14+ monocytes, CD3+ cells, CD4+ cells, CD8+ T cells, CD16+ CD56+ natural killer (NK) cells, and residual PLTs (p < 0.001), owing to higher centrifuge speed. The mean MNC purity of all components was more than 90 percent. By use of standard programs of either device, significant correlations (p < 0.01) between donor monocytes and preleukapheresis NK cell counts and the corresponding component cell yields were found.
  
  
  CONCLUSIONS
  Compared to the program modifications with lower centrifuge velocities the standard MNC programs were significantly more efficient regarding CD14+, CD3+, and CD16+ CD56+ cells. Enhanced centrifuge speed and inlet blood flow rate in MNC programs resulted in higher, similar composed MNC concentrations of the products.
authors.affiliation: Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg, Germany.
authors.forename: Erwin F
authors.initials: EF
authors.lastname: Strasser
authors.forename: Sandra
authors.initials: S
authors.lastname: Dittrich
authors.forename: Volker
authors.initials: V
authors.lastname: Weisbach
authors.forename: Robert
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ringwald
authors.forename: Susanne
authors.initials: S
authors.lastname: Achenbach
authors.forename: Jürgen
authors.initials: J
authors.lastname: Zingsem
authors.forename: Reinhold
authors.initials: R
authors.lastname: Eckstein
mesh_terms: D000328:Adult; D001772:Blood Cell Count; D017252:CD3 Complex / Q000032:analysis*; D006400:Hematocrit; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D011446:Prospective Studies; D016176:T-Lymphocyte Subsets / Q000166:cytology*
chemical_list: D017252:CD3 Complex; D018950:Lipopolysaccharide Receptors