title: Changes in pools of autoantibodies and anti-bacterial antibodies in patients suffering from recurrent infections of the urinary tract and undergoing bacterial immunization treatment.
abstract: Antibodies (Abs) (IgM, IgA, IgG and IgG subclasses) specific for several uropathogenic strains (Escherichia coli, Pseudomonas sp. and Klebsiella sp.) as well as anti-phospholipids, anti-beta2-glycoprotein I and anti-laminin antibodies were analyzed in the sera of 20 patients with long-lasting uncomplicated recurrent infections of the lower urinary tract who underwent immunization treatment with a mixture of heat-inactivated bacteria. Immunization had a dual effect: a marked prolongation of the infection-free period in more than half of tested patients (which could be related to the profiles of anti-bacterial antibodies), and the induction of a significant decrease in autoreactivity. The results obtained showed that prolonged infections resulted in a significant rise in IgG specific for phospholipids, beta2-glycoprotein I and mouse laminin. However, irrespective of the effect on urinary tract infection per se, immunization induced a noticeable decrease in reactivity toward those antigens (Ag). The most abundant autoantibodies prior to immunization treatment were of IgG2 subclass. A statistically significant decrease in phospholipid specific antibodies belonging to this subclass, and in the concentration of Y7 cross-reactive idiotope, registered only in the responder group; this indicates the significance of natural antibody pool involvement in a proper anti-bacterial immune response.
authors.affiliation: Institute of Immunology and Virology Torlak, Vojvode Stepe 458, 1152 Belgrade, Serbia and Montenegro.
authors.forename: Marijana
authors.initials: M
authors.lastname: Stojanović
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Inić-Kanada
authors.forename: Zoran
authors.initials: Z
authors.lastname: Popović
authors.forename: Irena
authors.initials: I
authors.lastname: Zivković
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Dimitrijević
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D001424:Bacterial Infections / Q000276:immunology* / Q000628:therapy; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D006023:Glycoproteins / Q000276:immunology; D016946:Gram-Negative Aerobic Rods and Cocci / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000097:blood; D007797:Laminin / Q000276:immunology; D008875:Middle Aged; D010743:Phospholipids / Q000276:immunology; D012008:Recurrence; D014552:Urinary Tract Infections / Q000276:immunology* / Q000628:therapy; D053482:beta 2-Glycoprotein I
chemical_list: D000907:Antibodies, Bacterial; D001323:Autoantibodies; D001428:Bacterial Vaccines; D006023:Glycoproteins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007797:Laminin; D010743:Phospholipids; D053482:beta 2-Glycoprotein I