Effect of mCOUP-TF1 deficiency on the glossopharyngeal and vagal sensory ganglia.
Immunohistochemistry for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase and calbindin D-28k was performed on the glossopharyngeal and vagal ganglia in mCOUP-TFI knockout mice to know the effect of its deficiency on different types of primary sensory neurons. In wild type and heterozygous mice, the glossopharyngeal and vagal ganglia contained abundant CGRP-, tyrosine hydroxylase- and calbindin D-28k-immunoreactive (IR) neurons. In the ganglia of mCOUP-TFI knockout mice, a 38% decrease of CGRP-IR neurons was detected. However, the number of tyrosine hydroxylase- or calbindin D-28k-neurons was not altered by the mCOUP-TFI deficiency. In the tongue of knockout mice, the number of CGRP-IR nerve fibers decreased compared to wild-type and heterozygous mice. The development of CGRP-IR petrosal neurons, which supply innervation of the tongue, may depend on mCOUP-TFI.
Ichikawa
H
H
Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. hiroichi@md.okayama-u.ac.jp
Lin
S-C
SC
Tsai
S Y
SY
Tsai
M-J
MJ
Sugimoto
T
T
D000818:Animals; D051836:COUP Transcription Factors; D064026:Calbindins; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000172:deficiency*; D017950:Ganglia, Sensory / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009475:Neurons, Afferent / Q000378:metabolism* / Q000473:pathology; D011987:Receptors, Steroid; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014018:Tissue Distribution; D014059:Tongue / Q000294:innervation; D014157:Transcription Factors / Q000172:deficiency*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D014630:Vagus Nerve / Q000378:metabolism / Q000473:pathology
D051836:COUP Transcription Factors; D064026:Calbindins; D004268:DNA-Binding Proteins; D011987:Receptors, Steroid; D064030:S100 Calcium Binding Protein G; D014157:Transcription Factors; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide