title: Comparative characterization of the oah2 gene homologous to the oah1 of Thermus thermophilus HB8.
abstract: The oah2 gene homologous to the oah1 of Thermus thermophilus HB8 was cloned and sequenced. It comprised 1,236 bp encoding a protein of 412 amino acid residues and was overexpressed. The gene product, also having O-acetyl-L-homoserine sulfhydrylase (EC 4.2.99.10) activity, was purified to homogeneity and characterized comparatively with the oah1 product. The two proteins shared many characteristics.
authors.affiliation: Department of Biotechnology, Faculty of Agriculture, Gifu University. iwamatom@cc.gifu-u.ac.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Iwama
authors.forename: Hideo
authors.initials: H
authors.lastname: Hosokawa
authors.forename: Weimin
authors.initials: W
authors.lastname: Lin
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawai
authors.forename: Shuzo
authors.initials: S
authors.lastname: Yamagata
mesh_terms: D000595:Amino Acid Sequence; D019757:Carbon-Oxygen Lyases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D006863:Hydrogen-Ion Concentration; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics
chemical_list: C018725:O-acetylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases