title: Melatonin blocks dexamethasone-induced immunosuppression in a seasonally breeding rodent Indian palm squirrel, Funambulus pennanti.
abstract: In vivo effect of dexamethasone and melatonin on immunomodulation has been investigated by studying the lymphocyte proliferation to the mitogen Con A from various lymphoid tissues including bone marrow cells of a seasonally breeding rodent adult male F. pennanti during reproductively inactive phase (October to December). During this phase, animal faces the maximum challenges of the nature (hypothermic stress, scarcity of food and shelter). Dexamethasone treatment (60 microg/day/squirrel) for 60 consecutive days significantly decreased the thymus and spleen activity. The lymphoid tissues mass, total leukocyte, lymphocyte count of peripheral blood, bone marrow and T-cell mediated immune function was also significantly suppressed following the dexamethasone treatment but treatment of melatonin (25 microg/squirrel/day) along with dexamethasone significantly restored the suppressed immune status in squirrels. Further, histological study of the thymus showed profound changes in the cellularity with a depletion of thymocytes in the cortex region of thymic lobules and increased in connective tissues and spindle cells. Melatonin treatment alone increased thymocytes density in thymic cortex, clearly suggesting that melatonin counteracted the experimentally induced immune stress by dexamethasone. Therefore, in nature during reproductively inactive phase of the squirrel a high level of melatonin was noted, that is required to combat nature's stress, which might have increased the internal level of corticoids.
authors.affiliation: Department of Zoology, Pineal Research Lab, Banaras Hindu University, Varanasi 221005, India. chaldar@banaras.ernet.in
authors.forename: Chandana
authors.initials: C
authors.lastname: Haldar
authors.forename: Seema
authors.initials: S
authors.lastname: Rai
authors.forename: Rajesh
authors.initials: R
authors.lastname: Singh
mesh_terms: D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D003907:Dexamethasone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007165:Immunosuppression Therapy; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects; D008221:Lymphoid Tissue / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008297:Male; D008550:Melatonin / Q000097:blood / Q000494:pharmacology*; D009929:Organ Size; D010081:Oxazolone / Q000276:immunology; D012589:Sciuridae / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D003208:Concanavalin A; D010081:Oxazolone; D003907:Dexamethasone; D008550:Melatonin; D003345:Corticosterone