title: Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry.
abstract: Quantitative matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) was applied for the screening of ten pyranose oxidase variants. Quantitative MALDI-ToF MS using isotopic labeled internal standards and ionic liquid matrices was performed using aliquots of enzyme reaction mixtures without prepurification steps. The results obtained were in good agreement with HPLC measurements. Analysis time was approx. 3.5 min for a five-fold determination. Thus, quantitative MALDI-ToF MS can be used as a tool for screening of sugar converting enzymes.
authors.affiliation: Technische Biochemie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
authors.forename: Ditte
authors.initials: D
authors.lastname: Bungert
authors.forename: Sabine
authors.initials: S
authors.lastname: Bastian
authors.forename: Dorothée M
authors.initials: DM
authors.lastname: Heckmann-Pohl
authors.forename: Friedrich
authors.initials: F
authors.lastname: Giffhorn
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002851:Chromatography, High Pressure Liquid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase