Vr2: a new apple scab resistance gene.
Reports from several European countries of the breakdown of the Vf resistance, the most frequently used source of resistance in breeding programs against apple scab, emphasize the urgency of diversifying the basis of apple scab resistance and pyramiding different apple scab resistances with the use of their associated molecular markers. GMAL 2473 is an apple scab resistant selection thought to carry the resistance gene Vr. We report the identification by BSA of three AFLP markers and one RAPD marker associated with the GMAL 2473 resistance gene. SSRs associated with the resistance gene were found by (1) identifying the linkage group carrying the apple scab resistance and (2) testing the SSRs previously mapped in the same region. One such SSR, CH02c02a, mapped on linkage group 2, co-segregates with the resistance gene. GAML 2473 was tested with molecular markers associated with other apple scab resistance genes, and accessions carrying known apple scab resistance genes were tested with the SSR linked to the resistance gene found in GMAL 2473. The results indicate that GMAL 2473 does not carry Vr, and that a new apple scab resistance gene, named Vr2, has been identified.
Patocchi
A
A
Plant Pathology Group, Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich, Switzerland. andrea.patocchi@ipq.agrl.ethz.ch
Bigler
B
B
Koller
B
B
Kellerhals
M
M
Gessler
C
C
D001203:Ascomycota; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017343:Genes, Plant / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D027845:Malus / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique