Molecular analysis of a Measles virus isolate from Brazil: a case originating in Japan.
This study reports on molecular analysis of a Measles virus (MV) isolate from a patient who was infected in Japan but showed symptoms after arriving to Brazil. This patient had typical clinical measles infection symptoms: fever, rash, cough and coryza. After isolating the virus in B95a cells, a fragment of the nucleoprotein (N) gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subjected to direct nucleotide sequencing. The sequence data showed that the MV isolate of concern is of the D5 genotype.
Oliveira
M I
MI
Instituto Adolfo Lutz, São Paulo, Brasil. olive40@uol.com.br
Curti
S P
SP
Figueiredo
C A
CA
Sardinha
A M A
AM
Sallum
M A M
MA
Durigon
E L
EL
D001938:Brazil; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008457:Measles / Q000453:epidemiology* / Q000821:virology; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
D009698:Nucleoproteins; D012367:RNA, Viral