title: Molecular cloning of a cysteine proteinase cDNA from the cotton boll weevil Anthonomus grandis (Coleoptera: Curculionidae).
abstract: The cotton boll weevil (Anthonomus grandis) causes severe cotton crop losses in North and South America. This report describes the presence of cysteine proteinase activity in the cotton boll weevil. Cysteine proteinase inhibitors from different sources were assayed against total A. grandis proteinases but, unexpectedly, no inhibitor tested was particularly effective. In order to screen for active inhibitors against the boll weevil, a cysteine proteinase cDNA (Agcys1) was isolated from A. grandis larvae using degenerate primers and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed significant homologies with other insect cysteine proteinases. Northern blot analysis indicated that the mRNA encoding the proteinase was transcribed mainly in the gut of larvae. No mRNA was detected in neonatal larvae, pupae, or in the gut of the adult insect, suggesting that Agcys1 is an important cysteine proteinase for larvae digestion. The isolated gene will facilitate the search for highly active inhibitors towards boll weevil larvae that may provide a new opportunity to control this important insect pest.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Final W5, Brasília, Brazil.
authors.forename: Osmundo Brilhante
authors.initials: OB
authors.lastname: De Oliveira Neto
authors.forename: João Aguiar Nogueira
authors.initials: JA
authors.lastname: Batista
authors.forename: Daniel John
authors.initials: DJ
authors.lastname: Rigden
authors.forename: Octávio Luiz
authors.initials: OL
authors.lastname: Franco
authors.forename: Rodrigo Rocha
authors.initials: RR
authors.lastname: Fragoso
authors.forename: Ana Carolina Santos
authors.initials: AC
authors.lastname: Monteiro
authors.forename: Rose Gomes
authors.initials: RG
authors.lastname: Monnerat
authors.forename: Maria Fátima
authors.initials: MF
authors.lastname: Grossi-De-Sa
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D014018:Tissue Distribution; D048889:Weevils / Q000201:enzymology* / Q000235:genetics
chemical_list: D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D012333:RNA, Messenger; D003546:Cysteine Endopeptidases