Biased hypermutagenesis associated with mutations in an untranslated hairpin of an RNA virus.
The mutation frequency of Turnip crinkle virus can increase 12-fold without inducing error catastrophe. Lesions in a hairpin repressor frequently reverted and led to second-site alterations biased for specific mutations. These results suggest that the hairpin may also function as an RNA chaperone to properly fold the RNA-dependent RNA polymerase.
McCormack
John C
JC
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Simon
Anne E
AE
D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D029688:Brassica napus / Q000821:virology; D019184:Carmovirus / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017354:Point Mutation; D016337:RNA, Catalytic; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D016337:RNA, Catalytic; D012367:RNA, Viral; C418481:hairpin ribozyme; D012324:RNA-Dependent RNA Polymerase