Inhibition of HIV-1 TAR RNA-Tat peptide complexation using poly(acrylic acid).
HIV-1 is regulated at the transcriptional level by the interaction of Tat protein with the transactivation responsive region (TAR) RNA, a 59-base stem-loop structure located at the 5'-end of all nascent HIV-1 transcripts. Here, by targeting the Tat peptide, we found that negatively charged poly(acrylic acid) (PAA) had high affinity with Tat peptide and could inhibit the interaction of TAR with Tat. Therefore, PAA could block HIV replication by binding to Tat not to TAR RNA, providing a new thinking for the design of novel anti-HIV drugs.
Zhao
Hong
H
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Li
Jinru
J
Jiang
Long
L
D000180:Acrylic Resins / Q000737:chemistry*; D001665:Binding Sites; D015696:Gene Products, tat / Q000737:chemistry* / Q000648:ultrastructure*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry* / Q000648:ultrastructure; D054322:tat Gene Products, Human Immunodeficiency Virus
D000180:Acrylic Resins; D015696:Gene Products, tat; D046911:Macromolecular Substances; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus; C006903:carbopol 940