[The effects of DPPH on cochlear microcirculation].
OBJECTIVE
To investigate the effects of free radical, 1, 1-Diphenyl-2-picylhydrazyl(DPPH), on cochlear blood flow.


METHODS
Twenty guinea pigs were divided into 3 groups at random, 6 for control group, 6 for 1 mmol/L of DPPH group and 8 for 0.1 mmol/L of DPPH group. 2 microliters vehicle or drugs were dropped into round window membrane. Cochlear microcirculation was monitored by laser Doppler flowmeter and the mean arterial blood flow(MABP), which transferred by pressure conductor sensor and preamplifier, was simutaneously recorded by a computer.


RESULTS
MABP was stable throught the experiment. Cochlear blood flow increased 12.49% (P < 0.05) in 1 mmol/L group, and decreased 3.02% (P < 0.05). In control group cochlear microcirculation had no significant changes.


CONCLUSIONS
DPPH has effects on cochlear microcirculation. The mechanism still need to be explored later.
Chen
Min
M
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Kong
Weijia
W
D000818:Animals; D001713:Biphenyl Compounds; D001783:Blood Flow Velocity / Q000187:drug effects; D003051:Cochlea / Q000098:blood supply*; D005260:Female; D005609:Free Radicals / Q000494:pharmacology; D006168:Guinea Pigs; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D010851:Picrates / Q000494:pharmacology*; D011897:Random Allocation; D012405:Round Window, Ear
D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl