title: Thromboelastography used to assess coagulation during treatment with molecular adsorbent recirculating system.
abstract: Coagulopathy is a life-threatening complication of liver cirrhosis. We describe the effect of molecular adsorbent recirculating system (MARS), a cell-free dialysis technique, on the blood coagulation of cirrhotic patients. From February 2002 to July 2002, nine patients--five males (55.5%) and four females (44.4%), age 47-70 yr (median 56)--underwent 12 courses (4-7 sessions each) of MARS. Patients were treated for the following indications: six (66.6%) acute-on-chronic hepatic failure, three (33.3%) intractable pruritus. Platelet count, prothrombin time (PT), international standardized ratio and thromboelastography were measured before and after each MARS session. Coagulation factors II, V, VII, VIII, IX, X, XI, XII, XIII, von Willebrand, lupus anticoagulant, protein C, protein S, antithrombin III, plasminogen, alpha 2 antiplasmin, D-dimer, fibrin monomers, complement, and C(1) inactivator were measured before and at the end of each MARS treatment. We found a statistically significant difference (p < 0.05) in the platelet count, PT, all the thromboelastograph variables (reaction and constant time, alpha angle, and maximal amplitude), factor VIII, von Willebrand, and D-dimer, when measured before and after MARS. Previous reports have shown amelioration of blood coagulation following MARS treatments. However, we document that MARS induces coagulopathy through a platelet-mediated mechanism, whereby platelet may be mechanically destroyed during the passage of blood through the filters and lines. An alternative postulated mechanism is an immune-mediated platelet disruption - coagulopathy.
authors.affiliation: Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA. cataldo.doria@mail.tju.edu
authors.forename: Cataldo
authors.initials: C
authors.lastname: Doria
authors.forename: Lucio
authors.initials: L
authors.lastname: Mandalà
authors.forename: Jan D
authors.initials: JD
authors.lastname: Smith
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Caruana
authors.forename: Victor L
authors.initials: VL
authors.lastname: Scott
authors.forename: Salvatore
authors.initials: S
authors.lastname: Gruttadauria
authors.forename: Mario
authors.initials: M
authors.lastname: Magnone
authors.forename: Ignazio R
authors.initials: IR
authors.lastname: Marino
mesh_terms: D000368:Aged; D001777:Blood Coagulation; D001778:Blood Coagulation Disorders / Q000209:etiology; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000503:physiopathology* / Q000628:therapy*; D017114:Liver Failure, Acute / Q000628:therapy*; D019164:Liver, Artificial; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D012189:Retrospective Studies; D013916:Thrombelastography; D014462:Ultrafiltration
chemical_list: D008567:Membranes, Artificial