Direct multicomponent analysis of beer samples constituents using micellar electrokinetic capillary chromatography.
A capillary electrophoretic method was developed using micellar electrokinetic capillary chromatography (MEKC) with diode-array detection to analyze simultaneously 26 beer constituents in a single procedure, including alcohols, iso-alpha-acids, amino acids, flavonoids, isoflavonoids, a vitamin, purine and pyrimidine bases. After filtration, sample components were separated with an uncoated capillary and a 25 mM sodium borate and 110 mM SDS buffer at pH 10.5. Analyses were run at 14 kV and 8 s of hydrodynamic injection with UV detection at 210 nm and 270 nm. The proposed method was successfully applied to the direct determination of beer constituents without any sample cleanup procedures.
Cortacero-Ramírez
Sonia
S
Research Laboratories of Grupo Cervezas Alhambra S.L., Faculty of Sciences, University of Granada, Spain.
Segura-Carretero
Antonio
A
Cruces-Blanco
Carmen
C
Hernáinz-Bermúdez de Castro
Miguel
M
Fernández-Gutiérrez
Alberto
A
D000438:Alcohols / Q000032:analysis* / Q000737:chemistry; D001515:Beer / Q000032:analysis*; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary; D005374:Filtration; D011687:Purines / Q000032:analysis* / Q000737:chemistry; D011743:Pyrimidines / Q000032:analysis* / Q000737:chemistry; D014815:Vitamins / Q000032:analysis* / Q000737:chemistry
D000438:Alcohols; D002021:Buffers; D011687:Purines; D011743:Pyrimidines; D014815:Vitamins; C030986:pyrimidine; C030985:purine