title: Application of oil refinery waste in the biosynthesis of glycolipids by yeast.
abstract: Candida antarctica or Candida apicola synthesized surfactants (glycolipids) in the cultivation medium supplemented with oil refinery waste, either with soapstock (from 5.0% to 12.0% v/v) or post-refinery fatty acids (from 2.0% to 5.0% v/v). The efficiency of glycolipids synthesis was determined by the amount of waste supplemented to the medium and was from 7.3 to 13.4 g/l and from 6.6 to 10.5 g/l in the medium supplemented with soapstock and post-refinery fatty acids, respectively. The studied yeast also synthesized glycolipids in the non-supplemented medium however, by the enrichment of medium with the oil refinery waste, a 7.5-8.5-fold greater concentration of glycolipids was obtained in the post-culture liquid then in the medium without addition of oil refinery waste. The yeast synthesized from 6.6 to 10.3 g dry biomass/l and the intra-cellular fat content was from 16.8% to 30.2%. The efficiency of glycolipids synthesis was determined by yeast species, medium acidity and culture period. The surface tension of the post-culture liquid separated from yeast biomass was reduced to 35.6 mN/m, which corresponded to the surface tension obtained at the critical micelle concentration of glycolipids.
authors.affiliation: Chair of Food Biotechnology, University of Warmia and Mazury in Olsztyn, J. Heweliusz St. 1, 10-718, Poland. wlodzimierz.bednarski@uwm.edu.pl
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Bednarski
authors.forename: Marek
authors.initials: M
authors.lastname: Adamczak
authors.forename: Jan
authors.initials: J
authors.lastname: Tomasik
authors.forename: Mariusz
authors.initials: M
authors.lastname: Płaszczyk
mesh_terms: D002175:Candida / Q000378:metabolism*; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis*; D007218:Industrial Microbiology / Q000379:methods*; D007220:Industrial Waste; D010578:Petroleum / Q000378:metabolism*; D012915:Soaps / Q000378:metabolism; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D006017:Glycolipids; D007220:Industrial Waste; D010578:Petroleum; D012915:Soaps; D013501:Surface-Active Agents