title: [Cloning and expression of fusion gene of transmembrane domain of human CD20 and g3pN in Escherichia coli].
abstract: OBJECTIVE
  To construct the expression vector containing transmembrane domain gene of human CD20 and g3pN1 gene and express the fusion gene high-efficiently in E.coli.
  
  
  METHODS
  The human CD20 gene and g3pN1 domain gene were amplified by RT-PCR and PCR from Daudi cells and M13K07 phage antibody library, respectively, and then cloned into expression vector pTIG-Trx. The constructed expression vector was expressed in E.coli.
  
  
  RESULTS
  Western blot analysis showed that expressed product could bind to anti-CD20 mAb.
  
  
  CONCLUSIONS
  The pTIG-GS has been constructed and expressed successfully in E.coli, which lays the foundation for further screening anti-CD20 antibody from phage antibody library.
authors.affiliation: Department of Biochemistry, Hebei North College, Zhangjiakou 075000, China. xyz700928@yahoo.com.cn
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Sun
authors.forename: Wei-yuan
authors.initials: WY
authors.lastname: Yu
authors.forename: Jian-zhen
authors.initials: JZ
authors.lastname: Cheng
mesh_terms: D018951:Antigens, CD20 / Q000096:biosynthesis / Q000235:genetics*; D002051:Burkitt Lymphoma / Q000473:pathology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D018951:Antigens, CD20; D018076:DNA, Complementary; D019151:Peptide Library; D011993:Recombinant Fusion Proteins