Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex.
Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.
Bayrer
James
J
Case Western Reserve University, Cleveland, Ohio, USA.
Wan
Zhuli
Z
Li
Biaoru
B
Weiss
Michael A
MA
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D004268:DNA-Binding Proteins; C070369:DSX protein, Drosophila; D029721:Drosophila Proteins; D014157:Transcription Factors