Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange.
To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.
Lanman
Jason
J
Department of Microbiology, University of Alabama at Birmingham, Birmingham Alabama 35294, USA.
Lam
TuKiet T
TT
Emmett
Mark R
MR
Marshall
Alan G
AG
Sakalian
Michael
M
Prevelige
Peter E
PE
D003903:Deuterium / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D006859:Hydrogen / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D006859:Hydrogen; D003903:Deuterium