title: [Effect of grape procyanidins on the apoptosis and mitochondrial transmembrane potential of thymus cells].
abstract: OBJECTIVE
  Investigating the effect of grape procyanidins(GPC) on the apoptosis and mitochondrial transmembrane potential of thymus cells damaged by H2O2.
  
  
  METHODS
  Thymus cells of mouse were cultured in vitro. The cells were pretreated by GPC and then damaged by 125 mumol/L H2O2 for two hours. The levels of apoptosis and mitochondrial transmembrane potential were analysed by flow cytometry.
  
  
  RESULTS
  Apoptosis rate and the level of mitochondrial transmembrane potential of positive group were (29.53 +/- 3.8)% and 27.24 +/- 2.2 (D value) respectively. After pretreatment of 50 mg/L GPC, the levels of apoptosis and mitochondrial transmembrane potential were (8.61 +/- 1.2)% and 87.55 +/- 4.7(D value). The differences between the two groups were significant (P < 0.05).
  
  
  CONCLUSIONS
  GPC can inhibit apoptosis and decrease of mitochondrial transmembrane potential of thymus cells damaged by oxygen free radical.
authors.affiliation: Nutrition Institute, Medical College of Qingdao University, Qingdao 266021, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jinyi
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000473:pathology; D018384:Oxidative Stress; D044945:Proanthocyanidins; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013950:Thymus Gland / Q000473:pathology* / Q000502:physiology; D027843:Vitis / Q000737:chemistry
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin; D006861:Hydrogen Peroxide