Characterization of rtSH3p13 gene encoding a development protein involved in vesicular traffic in spermiogenesis.
A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
Qiao
Yuan
Y
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Yang
Ju Xiang
JX
Zhang
Xiao Dong L
XD
Liu
Yu
Y
Zhang
Jian Chao
JC
Zong
Shu Dong
SD
Miao
Shi Ying
SY
Wang
Lin Fang
LF
Koide
Samuel S
SS
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D016466:CHO Cells / Q000378:metabolism; D002966:Clathrin / Q000737:chemistry / Q000502:physiology; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000737:chemistry / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002966:Clathrin; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D011506:Proteins; C495380:Sh3gl3 protein, rat; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases