title: Changes in bronchoalveolar lavage cells after intratracheal instillation of dimethyl selenide in mice.
abstract: CD-1 mice were exposed to a single intratracheal instillation of either 0.025 or 0.075 mg Se/kg wt of dimethyl selenide (DMSe). They were studied over 4 weeks to define the cellular inflammatory response of the airways to DMSe. Bronchoalveolar (BAL) lavage was used to collect the DMSe-induced inflammatory exudates. The DMSe instillation resulted in phlogistic responses that had the neutrophil as the main leukocyte; they were present in BAL samples, mostly at days 1 and 7. Macrophages were also increased during DMSe-induced inflammation. The lower DMSe dose resulted in an inflammatory reaction lasting for 2 weeks. Mice treated with the higher DMSe dose still showed elevated numbers of neutrophils and macrophages 4 weeks after instillation. DMSe did not change the number of lymphocytes harvested from the airways. An early increase in total protein of BAL, and late enhancement in lactate dehydrogenase was observed in mice treated with the high DMSe dose. We conclude that inhalation of DMSe triggers a moderate and dose-dependent inflammatory reaction in the mouse airways, and that this phlogistic reaction is likely to participate in the damage of respiratory epithelia that occurs upon DMSe inhalation.
authors.affiliation: ICBAS, Department of Anatomy and UMIB, Abel Salazar Institute for Biomedical Sciences, University of Porto, Portugal. duangrud@yahoo.com
authors.forename: Duangrudee
authors.initials: D
authors.lastname: Cherdwongcharoensuk
authors.forename: Suchart
authors.initials: S
authors.lastname: Upatham
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Oliveira
authors.forename: António
authors.initials: A
authors.lastname: Sousa Pereira
authors.forename: Artur P
authors.initials: AP
authors.lastname: AGuas
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology*; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008264:Macrophages; D008297:Male; D051379:Mice; D009504:Neutrophils; D016566:Organoselenium Compounds / Q000008:administration & dosage*; D011506:Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D016566:Organoselenium Compounds; D011506:Proteins; D007770:L-Lactate Dehydrogenase; C013503:dimethylselenide