Herpesviral-bacterial interrelationships in aggressive periodontitis.
BACKGROUND
Recent findings have begun to provide a basis for a causal link between herpesviruses and aggressive periodontitis. One theory is that herpesviruses cooperate with specific bacteria in the etiopathogenesis of the disease. This study examined whether the presence of herpesviruses [human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) type 1, herpes simplex virus (HSV) type 1 and 2] is associated with the presence of putative pathogenic bacteria (Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Campylobacter rectus, Actinobacillus actinomycetemcomitans) in aggressive periodontitis lesions.


METHODS
The study included 18 young adults with advanced periodontitis and 16 periodontally healthy subjects from Ankara, Turkey. Subgingival specimens pooled from two sites in each subject were collected by a periodontal curette. Qualitative polymerase chain reaction methodology was used to identify herpesviruses and bacteria. Chi-square tests were employed to determine statistical associations among herpesviruses, bacteria and periodontal disease.


RESULTS
HCMV, EBV-1 and HSV-1 were each detected in 72-78% of the aggressive periodontitis patients. HSV-2 occurred in 17% of the periodontitis patients. EBV-1 was detected in one periodontally healthy subject. The study bacteria occurred in 78-83% (P. gingivalis, T. forsythia, C. rectus) and in 44% (P. intermedia, A. actinomycetemcomitans) of the periodontitis samples, and in 0-19% of the samples from healthy periodontal sites. HCMV, EBV-1 and HSV-1 were positively associated with P. gingivalis, P. intermedia, T. forsythia and C. rectus, but not with A. actinomycetemcomitans. HSV-2 was not associated with any test bacteria.


CONCLUSIONS
These results support the notion that the clinical outcome of some types of severe periodontal infection depends on the presence of specific herpesviruses and bacterial pathogens. Our findings open the door to testing a variety of hypotheses regarding the deleterious aspects of combined herpesviral-bacterial infections in periodontal sites.
Saygun
Işil
I
Department of Periodontology, Gülhane Military Medical Academy, 06018 Etlik, Ankara, Turkey. saygunisil@hotmail.com
Kubar
Ayhan
A
Ozdemir
Atilla
A
Yapar
Mehmet
M
Slots
Jørgen
J
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology; D001439:Bacteroides / Q000502:physiology; D044883:Campylobacter rectus / Q000502:physiology; D016009:Chi-Square Distribution; D003587:Cytomegalovirus / Q000502:physiology; D003774:Dental Plaque Index; D005260:Female; D005881:Gingiva / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006564:Herpesviridae / Q000502:physiology*; D018259:Herpesvirus 1, Human / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000502:physiology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D008297:Male; D010512:Periodontal Index; D010518:Periodontitis / Q000382:microbiology* / Q000821:virology; D016966:Porphyromonas gingivalis / Q000502:physiology; D018713:Prevotella intermedia / Q000502:physiology; D011897:Random Allocation