Comparison of pulsatile and non-pulsatile cardiopulmonary bypass on regional renal blood flow in sheep.
OBJECTIVE
The purpose of the present study was to evaluate the effects of pulsatile cardiopulmonary bypass (CPB) on sheep regional renal blood flow by comparing pulsatile and non-pulsatile perfusion at two different flow rates.


METHODS
Seven female Suffolk sheep were used and the animals were perfused with pulsatile and non-pulsatile CPB at flow rates of 60 and 100 ml/min/kg. Regional renal blood flow was measured by the colored microsphere method. General linear model ANOVA was performed to analyze the data.


RESULTS
Regional renal blood flow was significantly higher in both outer and middle cortices of pulsatile CPB compared with non-pulsatile CPB (outer cortex: pulsatile CPB, 381+/-192 ml/min/100 g, non-pulsatile CPB, 255+/-151 ml/min/100g, p=0.002; middle cortex: pulsatile CPB, 239+/-114 ml/min/100 g, non-pulsatile CPB, 176+/-80 ml/min/100 g, p=0.02). The increase of flow rate from 60 to 100 ml/min/kg improved renal cortical blood flow significantly.


CONCLUSIONS
The regional renal blood flow was significantly higher in both outer and middle cortices of pulsatile CPB compared with the non-pulsatile CPB.
Nakamura
Kunihide
K
Department of Biomedical Engineering & Applied Therapeutics, The Cleveland Clinic Foundation, Cleveland, OH, USA. shiori@med.miyazaki-u.ac.jp
Harasaki
Hiroaki
H
Fukumura
Fumio
F
Fukamachi
Kiyotaka
K
Whalen
Robert
R
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002315:Cardiopulmonary Bypass; D005260:Female; D007672:Kidney Cortex / Q000098:blood supply*; D023421:Models, Animal; D008955:Models, Cardiovascular; D010477:Perfusion; D011673:Pulsatile Flow / Q000502:physiology*; D012039:Regional Blood Flow / Q000502:physiology; D012079:Renal Circulation / Q000502:physiology; D012756:Sheep