title: Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange.
abstract: To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, Birmingham Alabama 35294, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Lanman
authors.forename: TuKiet T
authors.initials: TT
authors.lastname: Lam
authors.forename: Mark R
authors.initials: MR
authors.lastname: Emmett
authors.forename: Alan G
authors.initials: AG
authors.lastname: Marshall
authors.forename: Michael
authors.initials: M
authors.lastname: Sakalian
authors.forename: Peter E
authors.initials: PE
authors.lastname: Prevelige
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D006859:Hydrogen / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D006859:Hydrogen; D003903:Deuterium