Development of primers for detection of meat and bone meal in ruminant feed and identification of the animal of origin.
The safe use of cattle feed free from meat and bone meal is an important prerequisite to prevent further spread of bovine spongiform encephalopathy. We designed primers to detect very small amounts of meat and bone meal in ruminant feed. Mitochondrial subunit 8 of the ATP synthase gene was used as a target sequence. PCR-based assays revealed amplification of DNA from mammals, ruminants, and individual species using these primers. The method allowed detection of the presence of meat and bone meal in ruminant feed from 0.1 to 0.01%. Sensitivity and effectiveness of the method for detecting prohibited animal proteins in ruminant feed was evaluated.
Kusama
T
T
Fertilizer and Feed Inspection Services, 2-1 Shintoshin, Chuo, Saitama, Saitama 330-9733, Japan. kusama@ffis.go.jp
Nomura
T
T
Kadowaki
K
K
D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D000818:Animals; D002417:Cattle; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis; D016643:Encephalopathy, Bovine Spongiform / Q000517:prevention & control* / Q000635:transmission; D005506:Food Contamination / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial