title: Genotypic analysis of sequential genital herpes simplex virus type 1 (HSV-1) isolates of patients with recurrent HSV-1 associated genital herpes.
abstract: Clinical recurrences of Herpes simplex virus type 1 (HSV-1)-associated genital herpes are thought to be caused by reactivation of latent endogenous HSV-1. However, the possibility of reinfection with exogenous HSV-1 cannot be excluded. This study aimed to determine the incidence of genital HSV-1 superinfection in patients by investigating the genotype of sequential HSV-1 isolates obtained from the same anatomical site of patients with clinical recurrences of genital HSV-1 recurrent genital herpes. Sequential genital HSV-1 isolates were genotyped by PCR amplification of the hypervariable regions located within the HSV-1 genes US1 and US12. Whereas the sequential HSV-1 isolates in 11 of the 13 patients studied had the same genotypes, the sequential isolates of 2 patients showed a different genotype. The data suggest that HSV-1-induced recurrent genital herpes can be associated with genital reinfection with an exogenous HSV-1 strain.
authors.affiliation: Departments of Dermatology and Venereology, Glasgow, Scotland.
authors.forename: R W
authors.initials: RW
authors.lastname: Roest
authors.forename: W F
authors.initials: WF
authors.lastname: Carman
authors.forename: J
authors.initials: J
authors.lastname: Maertzdorf
authors.forename: A
authors.initials: A
authors.lastname: Scoular
authors.forename: J
authors.initials: J
authors.lastname: Harvey
authors.forename: M
authors.initials: M
authors.lastname: Kant
authors.forename: W I
authors.initials: WI
authors.lastname: Van Der Meijden
authors.forename: Georges M G M
authors.initials: GM
authors.lastname: Verjans
authors.forename: A D M E
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000328:Adult; D005260:Female; D005838:Genotype; D006558:Herpes Genitalis / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012008:Recurrence; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: C105843:ICP47 protein, Herpes simplex virus; D017874:Immediate-Early Proteins; D014764:Viral Proteins