Efficient induction of cytotoxic T lymphocytes specific to hepatocellular carcinoma using HLA-A2-restricted MAGE-n peptide in vitro.
MAGE-n is a new member of MAGE gene family and has been demonstrated closely associated with hepatocellular carcinoma (HCC). In this study, MAGE-n-derived peptide-specific cytotoxic T lymphocytes (CTL) were induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with HLA-A2-restricted MAGE-n peptide-pulsed T2 cells. The induced CTLs exhibited specific lysis against T2 cells pulsed with the peptide and HLA-A2+ HCC cells expressing MAGE-n, while HLA-A2+ HCC cell lines that did not express MAGE-n could not be recognized by the CTLs. The peptide-specific activity was inhibited by anti-MHC class I monoclonal antibody. These results suggested the MAGE-n peptide could be a potential target of specific immunotherapy for HLA-A2 patients with HCC.
Dong
Hai-Long
HL
Department of Pathology, Fourth Military Medical University, Xi'an 710032, Shaanxi, China. donghl@fmmu.edu.cn
Sui
Yan-Fang
YF
Li
Zeng-Shan
ZS
Qu
Ping
P
Wu
Wen
W
Ye
Jing
J
Zhang
Xiu-Min
XM
Lu
Shao-Ying
SY
D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002460:Cell Line; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008113:Liver Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation; D009363:Neoplasm Proteins; D010455:Peptides; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; C095203:MAGEA6 protein, human; D009363:Neoplasm Proteins; D010455:Peptides