Transfer of European mink (Mustela lutreola) embryos into hybrid recipients.
The European mink is considered as a highly endangered Mustelidae species. The objective of this study was to explore the intriguing possibility of embryo transfer from European mink to closely related Mustelidae recipient females. To overcome interspecies pregnancy failure, embryos of European mink (Mustela lutreola) were transferred into hybrid females obtained after mating of European polecat (Mustela putorius) males and European mink (M. lutreola) females and vice versa. A total of 32 blastocysts were surgically flushed from the uteri of nine European mink donors and surgically transferred into six pseudopregnant hybrid recipients. One of the recipients received a single embryo and did not whelp. The remaining five recipients each received five to eight embryos and delivered kits. The overall success rate was 50% (16 kits/32 transferred embryos). For both male and female offspring, the average birth weight was lower in ET group when compared with naturally bred control population of European mink. The postnatal mortality rate was significantly higher in ET group as compared to controls: only 9 of 16 kits survived past the first week. At 10 days of age, the average weight for male offspring from the ET and control groups did not differ, although differences still persisted at this age for female offspring. At 3 months of age, the weight of male and female offspring in the ET group did not differ from European minks born after natural mating. We propose that transfer of European mink embryos to hybrid recipients be considered as a new experimental tool within the framework of ex situ approach conservation of this aboriginal European mustelid.
Amstislavsky
Sergei
S
Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Division, 630090, prosp. Lavrentjeva 10, Novosibirsk, Russia. amstis@bionet.nsc.ru
Aalto
Jussi
J
Järvinen
Mikko
M
Lindeberg
Heli
H
Valtonen
Maija
M
Zudova
Galina
G
Ternovskaya
Yulia
Y
D000818:Animals; D001724:Birth Weight; D001755:Blastocyst; D001947:Breeding; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D005289:Ferrets; D006824:Hybridization, Genetic; D008098:Litter Size; D008297:Male; D008907:Mink / Q000196:embryology*; D011247:Pregnancy; D020858:Tissue and Organ Harvesting / Q000662:veterinary