title: Reactivation of infectious simian virus 40 from normal human tissues.
abstract: In this study, 82 DNA samples of simian virus 40 (SV40)-positive human tumors and normal tissues were transfected into SV40-permissive monkey cells. SV40 wild-type strain 776 was reactivated from two DNA samples, derived from peripheral blood mononuclear cells (PBMCs) of a blood donor and from a vulvar tissue. SV40 reactivation was confirmed by obtaining rescue of SV40 from the DNA of the vulvar tissue in a second transfection experiment. This investigation indicates that infectious SV40 is present in normal human tissues and suggests that (i) PBMCs are probably vectors of SV40 to different tissues of the host and (ii) blood and sexual transmission may be routes of SV40 infection in humans, leading to (iii) virus spread in the human population.
authors.affiliation: Department of Morphology and Embryology, Section of Histology and Embryology, University of Ferrara, Ferrara, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Barbanti-Brodano
authors.forename: Fernanda
authors.initials: F
authors.lastname: Martini
authors.forename: Alfredo
authors.initials: A
authors.lastname: Corallini
authors.forename: Lorena
authors.initials: L
authors.lastname: Lazzarin
authors.forename: Cecilia
authors.initials: C
authors.lastname: Trabanelli
authors.forename: Beatrice
authors.initials: B
authors.lastname: Vignocchi
authors.forename: Nilla
authors.initials: N
authors.lastname: Calza
authors.forename: Laura
authors.initials: L
authors.lastname: Iaccheri
authors.forename: Cristina
authors.initials: C
authors.lastname: Morelli
authors.forename: Mauro
authors.initials: M
authors.lastname: Tognon
mesh_terms: D000818:Animals; D015342:DNA Probes; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000635:transmission*; D013539:Simian virus 40 / Q000302:isolation & purification*; D014162:Transfection; D014412:Tumor Virus Infections / Q000635:transmission*; D014844:Vulva / Q000821:virology*
chemical_list: D015342:DNA Probes