title: No association of C677T methylenetetrahydrofolate reductase and an endothelial nitric oxide synthase polymorphism with recurrent pregnancy loss.
abstract: OBJECTIVE
  It is controversial whether polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and the endothelial nitric oxide synthase (eNOS) are associated with recurrent pregnancy loss.
  
  
  METHODS
  We studied the frequency of the C677T polymorphism of MTHFR and a eNOS gene polymorphism, as well as the plasma levels of homocysteine and NO, in 85 cases with a history of two or more unexplained embryonal losses, 40 patients suffering fetal loss and 76 controls.
  
  
  RESULTS
  The frequency of the MTHFR gene T allele, which has been reported to be associated with miscarriages, in patients suffering fetal loss was rather significantly lower than in controls whereas there was no difference in the frequency of the eNOS gene A allele. There were no differences in the plasma homocysteine levels among the three groups. However, the NO concentrations in the embryonal loss and fetal loss groups were significantly higher than that in controls.
  
  
  CONCLUSIONS
  We conclude that the NO concentration but not the polymorphism of MTHFR and eNOS gene and hyperhomocysteinemia are associated with recurrent pregnancy loss in Japanese.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya City University Medical School, Nagoya, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Makino
authors.forename: Tamao
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Mayumi
authors.initials: M
authors.lastname: Sugiura-Ogasawara
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzumori
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzumori
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D003545:Cysteine / Q000235:genetics; D020964:Embryo Loss / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004727:Endothelium / Q000201:enzymology*; D005260:Female; D005838:Genotype; D006710:Homocysteine / Q000097:blood; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000737:chemistry / Q000235:genetics*; D009569:Nitric Oxide / Q000097:blood; D019001:Nitric Oxide Synthase / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011247:Pregnancy; D013912:Threonine / Q000235:genetics
chemical_list: D006710:Homocysteine; D013912:Threonine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D003545:Cysteine