mlstdbNet - distributed multi-locus sequence typing (MLST) databases.
BACKGROUND
Multi-locus sequence typing (MLST) is a method of typing that facilitates the discrimination of microbial isolates by comparing the sequences of housekeeping gene fragments. The mlstdbNet software enables the implementation of distributed web-accessible MLST databases that can be linked widely over the Internet.


RESULTS
The software enables multiple isolate databases to query a single profiles database that contains allelic profile and sequence definitions. This separation enables isolate databases to be established by individual laboratories, each customized to the needs of the particular project and with appropriate access restrictions, while maintaining the benefits of a single definitive source of profile and sequence information. Databases are described by an XML file that is parsed by a Perl CGI script. The software offers a large number of ways to query the databases and to further break down and export the results generated. Additional features can be enabled by installing third-party (freely available) tools.


CONCLUSIONS
Development of a distributed structure for MLST databases offers scalability and flexibility, allowing participating centres to maintain ownership of their own data, without introducing duplication and data integrity issues.
Jolley
Keith A
KA
The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, OX1 3SY, UK. keith.jolley@medawar.ox.ac.uk
Chan
Man-Suen
MS
Maiden
Martin C J
MC
D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D030561:Databases, Nucleic Acid; D012984:Software / Q000639:trends; D013045:Species Specificity
D004269:DNA, Bacterial