Efficacy of lytic peptide-bound magnetite nanoparticles in destroying breast cancer cells.
A 23-amino-acid synthetic lytic peptide (Hecate) was covalently linked to magnetite nanoparticles and the lytic peptide-bound nanoparticles were characterized by X-ray absorption near-edge structure spectroscopy, transmission electron microscopy, and electron diffraction. Investigation of magnetic properties with a superconducting quantum interference device (SQUID) magnetometer has shown a reduction in the saturation magnetization (Ms) of magnetite nanoparticles after binding with lytic peptide. An in vitro cell culture assay with breast cancer cell lines MDA-MB-435S revealed that the lytic peptide-bound magnetite nanoparticles were therapeutically active.
Kumar
Challa S
CS
Center for Advanced Microstructures and Devices, Louisiana State University, 6980 Jefferson Hwy, Baton Rouge, Louisiana 70806, USA.
Leuschner
Carola
C
Doomes
E E
EE
Henry
Larry
L
Juban
Martha
M
Hormes
Josef
J
D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron / Q000737:chemistry*; D007928:Lethal Dose 50; D008422:Materials Testing; D008555:Melitten / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology*; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D010087:Oxides; C098827:hecate 1; D008555:Melitten; D007501:Iron; D052203:Ferrosoferric Oxide