Inhibitory effect of Sihoga-Yonggol-Moryo-Tang on matrix metalloproteinase-2 and -9 activities and invasiveness potential of hepatocellular carcinoma.
A Korean traditional herbal formulation, Sihoga-Yonggol-Moryo-Tang (SGYMT), is being used for the treatment of many diseases such as tumor, neuropsychiatric, and vascular disorders. The extracts prepared from SGYMT and its herbal ingredients were assayed for the inhibitory effects on tumor-specific matrix metalloproteinases-2 and -9 (MMP-2/9) activities using gelatin zymography. The results showed that SGYMT decreased the gelatinolytic activities of MMP-2 and -9, which were secreted from SK-Hep1 cells. The IC(50) values of SGYMT against these enzymes were 85 and 145 micrograms ml(-1), respectively. The cytotoxocities of SGYMT and its ingredients on human hepatocellular carcinoma (HCC) cell, SK-Hep1 cells, were very low (IC(50)>150 micrograms ml(-1)) as measured by the XTT assay method. The extract were then investigated the inhibitory effect on the invasion of SK-Hep1 cells using matrigel precoated transwell chambers. The results showed that SGYMT effectively inhibited the invasion of SK-Hep1 cells as compared with the control groups. In addition, among herbal ingredients of SGYMT, the bark of Cinnamomum cassia BLUME (Cinnamomi Cortex) and roots of Scutellaria baicalensis GEORGI (Scutelleria Radix) have shown significant inhibition against MMP-2 and -9 activity and invasion of SK-Hep1 cells. From these results, it was suggested that SGYMT could be used as potential anti-tumor agent.
Ha
Ki-Tae
KT
National Research Laboratory for Glycobiology, Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine, Dongguk University, Sukjang-Dong 707, Kyungju City, Kyungbuk 780-714, Republic of Korea.
Kim
June-Ki
JK
Kang
Sung-Koo
SK
Kim
Dong-Wook
DW
Lee
Young-Choon
YC
Kim
Hyung-Min
HM
Kim
Cheorl-Ho
CH
D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018514:Plant Structures; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use
D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; C095444:saiko-ka-ryukotsu-borei-to; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9