Synthesis of oligodeoxynucleotides containing a single diastereoisomer of alpha-(N2-2'-deoxyguanosinyl)-N-desmethyltamoxifen.
A phosphoramidite chemical synthesis of oligodeoxynucleotides containing a diastereoisomer of (E)-alpha-(N(2)-deoxyguanosinyl)-N-desmethyltamoxifen, a major tamoxifen (TAM)-derived DNA adduct in animal and women treated with TAM, was described. The site-specifically modified oligodeoxynucleotide can be used for mutagenesis, DNA repair, and 3D structural studies and also as standard for quantitative analysis of TAM-DNA adducts in animal and human.
Laxmi
Y R Santosh
YR
Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-8651, USA.
Suzuki
Naomi
N
Kim
Sung Yeon
SY
Shibutani
Shinya
S
D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts; D003849:Deoxyguanosine; D006801:Humans; D008968:Molecular Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology*; D013237:Stereoisomerism; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000494:pharmacology
D018736:DNA Adducts; D009838:Oligodeoxyribonucleotides; D013629:Tamoxifen; D003849:Deoxyguanosine