Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis.
A marine green microalga, Platymonas subcordiformis, photo-synthetically generates H(2) but only transiently at a negligible yield when exposed to light after a period of dark anaerobic incubation. A protonophore uncoupler, carbonyl cyanide m-chlorophenylhrazone (CCCP) significantly increased the yield of H(2) photo-production. CCCP optimally at 15 microM gave 4.9 ml H(2) after 8 h light irradiation in 1 l algal cell culture at 1.8 x 10(6) cells ml(-1). The H(2) yield at 15 microM CCCP was increased by 240-fold when compared to the control. This improvement may be by CCCP disrupting the proton motive force thus facilitating proton transfer across the thylakoidal membrane.
Guan
Yingfu
Y
Marine Bioproducts Engineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China.
Zhang
Wei
W
Deng
Maicun
M
Jin
Meifang
M
Yu
Xingju
X
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D018462:Photobiology
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006859:Hydrogen