title: A soft lithographic approach to fabricate patterned microfluidic channels.
abstract: The control of surface properties and spatial presentation of functional molecules within a microfluidic channel is important for the development of diagnostic assays and microreactors and for performing fundamental studies of cell biology and fluid mechanics. Here, we present a simple technique, applicable to many soft lithographic methods, to fabricate robust microchannels with precise control over the spatial properties of the substrate. In this approach, the patterned regions were protected from oxygen plasma by controlling the dimensions of the poly(dimethylsiloxane) (PDMS) stamp and by leaving the stamp in place during the plasma treatment process. The PDMS stamp was then removed, and the microfluidic mold was irreversibly bonded to the substrate. The approach was used to pattern a nonbiofouling poly(ethylene glycol)-based copolymer or the polysaccharide hyaluronic acid within microfluidic channels. These nonbiofouling patterns were then used to fabricate arrays of fibronectin and bovine serum albumin as well as mammalian cells. In addition, further control over the deposition of multiple proteins onto multiple or individual patterns was achieved using laminar flow. Also, cells that were patterned within channels remained viable and capable of performing intracellular reactions and could be potentially lysed for analysis.
authors.affiliation: Division of Biological Engineering and Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E25-342, Cambridge, MA 02139, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Kahp Y
authors.initials: KY
authors.lastname: Suh
authors.forename: Sangyong
authors.initials: S
authors.lastname: Jon
authors.forename: George
authors.initials: G
authors.lastname: Eng
authors.forename: Judy
authors.initials: J
authors.lastname: Yeh
authors.forename: Guan-Jong
authors.initials: GJ
authors.lastname: Chen
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology*; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D011092:Polyethylene Glycols