pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates in Portugal.
The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were pyrazinamidase (PZase) negative. Four strains contained a wild-type pncA gene, and PZase activity was undetectable in two of these strains. Seven of the 18 identified pncA mutations found have not been described in previous studies.
Portugal
Isabel
I
Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Rua Prof. Gama Pinto, 1649-003 Lisbon, Portugal. isabel.portugal@ff.ul.pt
Barreiro
Luís
L
Moniz-Pereira
José
J
Brum
Laura
L
D000581:Amidohydrolases / Q000235:genetics*; D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D009154:Mutation / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011174:Portugal / Q000453:epidemiology; D011718:Pyrazinamide / Q000494:pharmacology*; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology
D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis