Analysis and control of proteolysis of recombinant proteins in Escherichia coli.
Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which are relevant for the production of recombinant proteins, are reviewed. Furthermore, various strategies to control the proteolysis of the recombinant proteins are presented. These strategies for control of proteolysis can be applied on various stages of the process: design of more stable protein, a modification of the host cell in respect to proteolytic activity, optimisation of cultivation and downstream processing. However, before implementing these measures the proteolysis rate should be measured in order to calculate a potential benefit of reduced proteolysis rate.
Rozkov
Aleksei
A
Department of Biotechnology, Royal Institute of Technology (KTH), Roslagstullsbacken 21, 10691 Stockholm, Sweden.
Enfors
Sven-Olof
SO
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018929:Cell Culture Techniques / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods; D006706:Homeostasis / Q000502:physiology; D006868:Hydrolysis; D018384:Oxidative Stress / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010450:Endopeptidases