Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes.
Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, ORFeome clones representing 1,282 Arabidopsis TF genes have been obtained in the Gateway high throughput cloning pENTR vector, including 411 genes whose annotation lack cDNA support. All the ORFeome inserts have also been mobilized into a yeast expression destination vector, with an estimated 85% rate of expressing the respective proteins. Sequence analysis of these clones revealed that 34 of them did not match with either the reported cDNAs or current predicted open-reading-frame sequences. Among those, novel alternative splicing of TF gene transcripts is responsible for the observed differences in at least five genes. However, those alternative splicing events do not appear to be differentially regulated among distinct Arabidopsis tissues examined. Lastly, expression of those TF genes in 17 distinct Arabidopsis organ types and the cultured cells was profiled using a 70-mer oligo microarray.
Gong
Wei
W
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, and the National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China.
Shen
Yun-Ping
YP
Ma
Li-Geng
LG
Pan
Yi
Y
Du
Yun-Long
YL
Wang
Dong-Hui
DH
Yang
Jian-Yu
JY
Hu
Li-De
LD
Liu
Xin-Fang
XF
Dong
Chun-Xia
CX
Ma
Li
L
Chen
Yan-Hui
YH
Yang
Xiao-Yuan
XY
Gao
Ying
Y
Zhu
Danmeng
D
Tan
Xiaoli
X
Mu
Jin-Ye
JY
Zhang
Da-Bing
DB
Liu
Yu-Le
YL
Dinesh-Kumar
S P
SP
Li
Yi
Y
Wang
Xi-Ping
XP
Gu
Hong-Ya
HY
Qu
Li-Jia
LJ
Bai
Shu-Nong
SN
Lu
Ying-Tang
YT
Li
Jia-Yang
JY
Zhao
Jin-Dong
JD
Zuo
Jianru
J
Huang
Hai
H
Deng
Xing Wang
XW
Zhu
Yu-Xian
YX
D017398:Alternative Splicing / Q000235:genetics; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D014157:Transcription Factors