A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing.
Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.
Diego
L Borja
LB
Laboratorio Fisiología Vegetal, Departamento BOS, Facultad de Biología, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n. E-33071, Oviedo, Spain.
Berdasco
María
M
Fraga
Mario F
MF
Cañal
M Jesús
MJ
Rodríguez
Roberto
R
Castresana
Carmen
C
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D028223:Pinus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D046988:Proteasome Endopeptidase Complex; D018749:RNA, Plant / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D009097:Multienzyme Complexes; D018749:RNA, Plant; D012335:RNA, Ribosomal; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases