title: Genetic identification and host range of the Spanish strains of Echinococcus granulosus.
abstract: Three strains of Echinococcus granulosus have been previously identified in Spain (namely 'sheep', 'horse' and 'pig'), but these Spanish strains have not been properly genotyped yet. The aim of the present research was to identify the genotype to which they correspond to. Cyst isolates were obtained from different host species, and the strain to which each belonged was established by analysis of its random amplified polymorphic DNA (RAPD) banding patterns. These results were compared to those obtained with the analysis of two mitochondrial fragment sequences (cytochrome oxidase 1 (CO1) and NADH dehydrogenase 1 (ND1)) from each isolate. The Spanish 'sheep' strain corresponded with the genotype 1 (G1) of the parasite, infecting Spanish sheep, cattle, goat, pig, wild boar and human; the Spanish 'horse' strain corresponded with the genotype 4 (G4), only infecting Spanish horses; and the Spanish 'pig' strain corresponded with the genotype 7 (G7), infecting Spanish goat, pig and wild boar. Goat, pig and wild boar can be infected by two genotypes, G1 and G7. This circumstance, and especially the possibility of sylvatic intermediate hosts serving as reservoirs of the G1 genotype of the parasite, must be taken into consideration by authorities in order to develop and evaluate effective anti-hydatidosis programmes.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n. 28040 Madrid, Spain.
authors.forename: K
authors.initials: K
authors.lastname: Daniel Mwambete
authors.forename: F
authors.initials: F
authors.lastname: Ponce-Gordo
authors.forename: C
authors.initials: C
authors.lastname: Cuesta-Bandera
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics; D000818:Animals; D002417:Cattle; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006041:Goats; D006736:Horses; D006801:Humans; D008354:Mannitol Dehydrogenases; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D012756:Sheep; D013030:Spain; D034421:Sus scrofa
chemical_list: D018509:DNA, Helminth; D000429:Alcohol Oxidoreductases; D008354:Mannitol Dehydrogenases; C116789:mannitol 2-dehydrogenase (NADP); D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV