The X protein of Borna disease virus regulates viral polymerase activity through interaction with the P protein.
Borna disease virus polymerase activity is negatively regulated by the viral X protein. Using a virus minireplicon system it was found that all X mutants that no longer interacted with the viral P protein failed to exhibit significant inhibitory activity. The action of X could further be neutralized by expression of a P fragment that contained the X interaction domain but lacked all domains known to mediate interaction with other viral proteins. X thus appears to regulate the activity of the Borna disease virus polymerase by targeting the polymerase cofactor P.
Poenisch
Marion
M
Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
Unterstab
Gunhild
G
Robert-Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
Wolff
Thorsten
T
Robert-Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
Staeheli
Peter
P
Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
Schneider
Urs
U
Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
D001891:Borna disease virus / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D016679:Genome, Viral; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases