title: Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA.
abstract: Small interfering RNA (siRNA)-induced RNA degradation has been used recently as an antivirus agent to inhibit specific virus replication. This report shows that 21 nt duplexes of siRNA of the influenza virus M gene can cause specific inhibition of influenza virus matrix (M1) protein expression in transfected 293T cells. Furthermore, it is shown that a lentivirus vector can be used to effectively deliver M gene siRNAs into Madin-Darby canine kidney cells and can cause specific inhibition of M1 protein expression and influenza virus replication. Therefore, lentivirus-mediated delivery of siRNA and gene silencing can be used in studying the specific functions of virus genes in replication and may have a potential therapeutic application.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
authors.forename: Eric Ka-Wai
authors.initials: EK
authors.lastname: Hui
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
authors.forename: Ee Ming
authors.initials: EM
authors.lastname: Yap
authors.affiliation: Departments of Microbiology, Immunology, and Molecular Genetics and Medicine, UCLA AIDS Institute, Los Angeles, CA 90095, USA.
authors.forename: Dong Sung
authors.initials: DS
authors.lastname: An
authors.affiliation: Departments of Microbiology, Immunology, and Molecular Genetics and Medicine, UCLA AIDS Institute, Los Angeles, CA 90095, USA.
authors.forename: Irvin S Y
authors.initials: ISY
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
authors.forename: Debi P
authors.initials: DP
authors.lastname: Nayak
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007668:Kidney; D016086:Lentivirus / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral; D014763:Viral Matrix Proteins