A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants.
Salicylic acid (SA)-mediated host immunity plays a central role in combating microbial pathogens in plants. Inactivation of SA-mediated immunity, therefore, would be a critical step in the evolution of a successful plant pathogen. It is known that mutations in conserved effector loci (CEL) in the plant pathogens Pseudomonas syringae (the Delta CEL mutation), Erwinia amylovora (the dspA/E mutation), and Pantoea stewartii subsp. stewartii (the wtsE mutation) exert particularly strong negative effects on bacterial virulence in their host plants by unknown mechanisms. We found that the loss of virulence in Delta CEL and dspA/E mutants was linked to their inability to suppress cell wall-based defenses and to cause normal disease necrosis in Arabidopsis and apple host plants. The Delta CEL mutant activated SA-dependent callose deposition in wild-type Arabidopsis but failed to elicit high levels of callose-associated defense in Arabidopsis plants blocked in SA accumulation or synthesis. This mutant also multiplied more aggressively in SA-deficient plants than in wild-type plants. The hopPtoM and avrE genes in the CEL of P. syringae were found to encode suppressors of this SA-dependent basal defense. The widespread conservation of the HopPtoM and AvrE families of effectors in various bacteria suggests that suppression of SA-dependent basal immunity and promotion of host cell death are important virulence strategies for bacterial infection of plants.
DebRoy
Sruti
S
Cell and Molecular Biology Program and Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Thilmony
Roger
R
Kwack
Yong-Bum
YB
Nomura
Kinya
K
He
Sheng Yang
SY
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000502:physiology; D017124:Conserved Sequence / Q000235:genetics; D044084:Erwinia amylovora / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D005936:Glucans / Q000378:metabolism; D007113:Immunity, Innate / Q000502:physiology*; D020935:MAP Kinase Signaling System; D027845:Malus / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009336:Necrosis; D020636:Pantoea / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D020156:Salicylic Acid / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D005936:Glucans; C048306:callose; D020156:Salicylic Acid