Auto antibodies against oxidized low density lipoprotein in severe preeclampsia.
BACKGROUND
To study autoantibody titres against oxidized low density lipoprotein in preeclamsia.


METHODS
Ten millimeters of heparinized blood samples were collected from 20 primigravidae with severe preeclamsia (study group) and 20 gestation-matched normotensive primigravidae (control group). Concentration of malondialdehyde, metabolite of lipid peroxidation were measured in sera by HPLC and autoantibodies against oxidized low density lipoproteins (obtained after oxidation with 2 mm CuSO(4)) were determined by ELISA. Statistical analysis was performed by Student's t-test and chi(2) test.


RESULTS
Mean triglyceride levels were significantly (P < 0.001) higher in the study group (193.20 +/- 31.16 mg/dL) compared to the control group (170.60 +/- 23.2 mg/dL). Mean plasma lipid per oxide levels were not significantly different between the study (4.45 +/- 1.28 mmol/mL) and control (3.88 +/- 0.99 mmol/mL) groups. The majority of women in both groups had low antibody titres (<1.32) against low density lipoprotein. Six women (30%) of the study group and four (20%) of the control group had high autoantibody titres (>/=1.32). In preeclamptic women, diastolic blood pressure, the amount of urinary protein excretion and the plasma level of urea were significantly higher (P < 0.05) in patients with higher auto antibody titre.


CONCLUSIONS
Titres of autoantibodies to oxidized low density lipoprotein were similar in normotensive and preeclamptic women. In preeclamptic women, titres correlated positively with the severity of preeclampsia.
Jain
Meenakshi
M
Department of Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research, Chandhigarh, India.
Sawhney
Harjeet
H
Aggarwal
Neelam
N
Vashistha
Kala
K
Majumdhar
Siddarth
S
D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000276:immunology*; D008315:Malondialdehyde / Q000276:immunology*; D010084:Oxidation-Reduction; D011225:Pre-Eclampsia / Q000097:blood / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D012720:Severity of Illness Index; D014280:Triglycerides / Q000097:blood
D001323:Autoantibodies; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; D002784:Cholesterol