Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan.
Copper is used in treatment mixtures to control fungal diseases in vineyards plants. High concentrations of copper are inducing antioxidant stress in some aquatic ecosystems, and potential bioaccumulation in aquatic organisms has prompted the demand for alternative use of low toxic molecules in culture treatments. Chitosan is a biomolecule with antifungal and heavy metal ion chelating properties that may be used as a biopesticide. In this study, we investigate the potential toxicity of chitosan for aquatic animal health, alone or associated with copper. Carp (Cyprinus carpio L.) were exposed to different chitosan concentrations (from 37.5 to 375 mg/l) or to two sublethal copper concentrations (0.1 and 0.25 mg/l) or to chitosan and copper (75 and 0.1 mg/l, respectively). Antioxidant enzyme activities were enhanced in chitosan treated fish after 4 days and depressed after 8 days. This phenomenon indicated a non-negligible toxicity of chitosan in fish physiology. However, the mixture copper-chitosan seems to induce a lower degree of oxidative stress than each fungicide alone. These observations show that chitosan is a potentially noxious molecule for some fish and any industrial and/or agricultural uses of this compound will have to address this problem.
Dautremepuits
Claire
C
Laboratory of Eco-Toxicology, University of Reims Champagne-Ardenne, BP 1039, Moulin de la Housse, 51687 Reims, Cedex 2, France. claire.dautremepuits@univ-reims.fr
Paris-Palacios
Séverine
S
Betoulle
Stéphane
S
Vernet
Guy
G
D000818:Animals; D000935:Antifungal Agents / Q000633:toxicity; D002347:Carps / Q000033:anatomy & histology / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D048271:Chitosan / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D014873:Water Pollutants / Q000633:toxicity*
D000935:Antifungal Agents; D014873:Water Pollutants; D003300:Copper; D048271:Chitosan; D002374:Catalase; D005982:Glutathione Transferase