title: Bacterial formyl peptides affect the innate cellular antimicrobial responses of larval Galleria mellonella (Insecta: Lepidoptera).
abstract: The non-self cellular (hemocytic) responses of Galleria mellonella larvae, including the attachment to slides and the removal of the bacteria Xenorhabdus nematophila and Bacillus subtilis from the hemolymph, were affected by N-formyl peptides. Both N-formyl methionyl-leucyl-phenylalanine (fMLF) and the ester derivative decreased hemocyte adhesion in vitro, and both elevated hemocyte counts and suppressed the removal of both X. nematophila and B. subtilis from the hemolymph in vivo. The amide derivative and the antagonist tertiary-butoxy-carbonyl-methionyl-leucyl-phenylalanine (tBOC) increased hemocyte attachment to glass. The fMLF suppressed protein discharge from monolayers of granular cells with and without bacterial stimulation, while tBOC stimulated protein discharge. The peptide tBOC offset the effects of fMLF in vitro and in vivo. This is the first report implying the existence of formyl peptide receptors on insect hemocytes in which the compounds fMLF and tBOC inhibited and activated hemocyte activity, respectively.
authors.affiliation: Department of Natural Resources Sciences, Macdonald Campus of McGill University, Saint Anne de Bellevue, QC, Canada.
authors.forename: Thiery B C
authors.initials: TB
authors.lastname: Alavo
authors.forename: Gary B
authors.initials: GB
authors.lastname: Dunphy
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry / Q000502:physiology*; D006434:Hemocytes / Q000382:microbiology* / Q000502:physiology; D007814:Larva / Q000187:drug effects* / Q000382:microbiology; D007915:Lepidoptera / Q000254:growth & development* / Q000382:microbiology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D020593:Xenorhabdus / Q000737:chemistry / Q000502:physiology*
chemical_list: D010455:Peptides; D009240:N-Formylmethionine Leucyl-Phenylalanine