title: Characteristics of testicular lesions in mice infected with a low dose of encephalomyocarditis (EMC) virus.
abstract: We investigated the characteristics of testicular lesions induced in mice with a low dose (10 plaque forming units/mouse) of the D variant of encephalomyocarditis (EMC-D) virus. The virus titers of blood and testis peaked at 5 days post-inoculation (5 DPI) and were no longer detected at 14 DPI. The IFN-gamma and iNOS mRNAs expression in the testis and spleen detected by RT-PCR was prominently elevated at 7 DPI, although the expression level of TNF-alpha mRNA was not affected. Signals of viral RNA were clearly detected in degenerative germinal epithelia (in situ hybridization) at 7 DPI, which were surrounded by a small number of macrophages and a few CD4 + T cells and CD8 + T cells (immunohistochemistry). Signals were no longer detected at 21 DPI when seminiferous tubules were highly degenerative and accompanied with infiltration of many macrophages and a small numbers of CD4 + T cells and CD8 + T cells. At 35 DPI, marked atrophy of germinal epithelia composed of Sertoli cells alone was observed, and there were almost no infiltrating cells detected. The present results suggest that macrophages may play an important role in the development of testicular lesions induced in mice with a low dose of EMC-D.
authors.affiliation: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Aito
authors.initials: A
authors.lastname: Yamanouchi-Ueno
authors.forename: Yumi
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Kunio
authors.initials: K
authors.lastname: Doi
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000276:immunology / Q000473:pathology* / Q000821:virology; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D009920:Orchitis / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism / Q000473:pathology / Q000821:virology; D013737:Testis / Q000378:metabolism / Q000473:pathology* / Q000821:virology
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse