title: CNS grafts of rat choroid plexus protect against cerebral ischemia in adult rats.
abstract: The present study examined the neuroprotective effects of choroid plexus isolated from adult rats and encapsulated within alginate microcapsules. In vitro, conditioned media from cultured choroid plexus produced a marked, dose-dependent protection of embryonic cortical neurons against serum deprivation-induced cell death. In vivo studies demonstrated that a one-hour middle cerebral artery occlusion in adult Wistar rats produced profound motor and neurological impairments 1-3 days after stroke. In contrast, stroke animals transplanted with encapsulated choroid plexus cells displayed a significant reduction in both motor and neurological abnormalities. Histological analysis 3 days post-transplantation revealed that choroid plexus transplants significantly decreased the volume of striatal infarction. This is the first report demonstrating the therapeutic potential of transplanted choroid plexus for stroke.
authors.affiliation: Department of Neurology/Institute of Molecular Medicine and Genetics/School of Graduate Studies, BI-3080, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912-3200, USA. cborlongan@mail.mcg.edu
authors.forename: C V
authors.initials: CV
authors.lastname: Borlongan
authors.forename: S J M
authors.initials: SJ
authors.lastname: Skinner
authors.forename: M
authors.initials: M
authors.lastname: Geaney
authors.forename: A V
authors.initials: AV
authors.lastname: Vasconcellos
authors.forename: R B
authors.initials: RB
authors.lastname: Elliott
authors.forename: D F
authors.initials: DF
authors.lastname: Emerich
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001519:Behavior; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D016380:Brain Tissue Transplantation / Q000379:methods*; D002214:Capsules / Q000627:therapeutic use; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D002544:Cerebral Infarction / Q000473:pathology / Q000601:surgery; D002831:Choroid Plexus / Q000166:cytology* / Q000502:physiology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D006085:Graft Survival / Q000502:physiology; D008124:Locomotion / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D002214:Capsules; D017077:Culture Media, Conditioned; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium