title: Overdiagnosis of amoebiasis in the absence of Entamoeba histolytica among patients presenting with diarrhoea in Wonji and Akaki, Ethiopia.
abstract: To confirm the high reported incidence of intestinal amoebiasis among study participants at 2 cohort sites in Ethiopia where an HIV/AIDS study is taking place, stool samples of 232 patients with complaints of diarrhoea were examined for the presence of Entamoeba histolytica and E. dispar DNA between April and December 2001. By microscopy, 91 (39%) of the study participants were reported to harbour Entamoeba trophozoites and/or four-nucleated cysts. Using specific E. histolytica and E. dispar DNA amplification and detection, none of the study participants were found to be infected with E. histolytica and only 21 (9%) with E. dispar. The consequences of the overdiagnosis of E. histolytica are briefly discussed.
authors.affiliation: Ethiopian Health and Nutrition Research & Ethio-Netherlands AIDS Research Project, Addis Ababa, Ethiopia. amha@enarp.com
authors.forename: Amha
authors.initials: A
authors.lastname: Kebede
authors.forename: Jaco
authors.initials: J
authors.lastname: Verweij
authors.forename: Wendelien
authors.initials: W
authors.lastname: Dorigo-Zetsma
authors.forename: Eduard
authors.initials: E
authors.lastname: Sanders
authors.forename: Tsehaynesh
authors.initials: T
authors.lastname: Messele
authors.forename: Lisette
authors.initials: L
authors.lastname: van Lieshout
authors.forename: Beyene
authors.initials: B
authors.lastname: Petros
authors.forename: Ton
authors.initials: T
authors.lastname: Polderman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis; D003967:Diarrhea / Q000469:parasitology*; D004404:Dysentery, Amebic / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D005002:Ethiopia / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies
chemical_list: D016054:DNA, Protozoan