Augmented taurine release is not the mechanism of ischemic preconditioning's cardioprotection.
In ischemic preconditioning (IPC) a brief ischemic period protects the heart from a subsequent ischemic insult by an unknown mechanism. Osmotic swelling has been proposed to be a major cause of cell death when ischemic tissue is reperfused. The present study tests whether the preconditioned heart during reperfusion might release more taurine, an important osmolyte in the cardiac myocytes, to decrease cellular osmolarity, oppose swelling, and preserve viability. We collected the coronary effluent from isolated rabbit hearts for 10 min before and 10 min after preconditioning with 5 min of global ischemia. The heart then experienced 15 min of global ischemia and effluent was collected during reperfusion for 40 min. A control group was studied similarly but without the preconditioning ischemia. Fifteen min of ischemia was chosen to avoid any taurine release caused by ischemic cell death. Taurine was measured with HPLC. In the IPC group there was a postischemic release over baseline of 5.09 +/- 1.51 micromol (approx 3.3% of the total taurine pool), whereas in the control group the release was not significantly different, 5.72 +/- 1.67 micromol. The percent of the taurine pool lost from each heart during reperfusion was calculated based on an assumption of a total content of 20 microM taurine/gm wet weight. Since the amount of taurine released by the isolated rabbit heart following ischemia was not different in preconditioned and non-preconditioned hearts, we conclude that reduced swelling through taurine release is not the mechanism of the cardioprotective effects of IPC.
Krieg
T
T
Department of Physiology, University of South Alabama, College of Medicine, Mobile, Alabama, USA.
Yang
X-M
XM
Downey
J M
JM
Schaffer
S W
SW
Cohen
M V
MV
D000818:Animals; D005260:Female; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D011817:Rabbits; D013654:Taurine / Q000378:metabolism* / Q000494:pharmacology
D013654:Taurine