title: The neurobiology of the ascidian tadpole larva: recent developments in an ancient chordate.
abstract: With little more than 330 cells, two thirds within the sensory vesicle, the CNS of the tadpole larva of the ascidian Ciona intestinalis provides us with a chordate nervous system in miniature. Neurulation, neurogenesis and its genetic bases, as well as the gene expression territories of this tiny constituency of cells all follow a chordate plan, giving rise in some cases to frank structural homologies with the vertebrate brain. Recent advances are fueled by the release of the genome and EST expression databases and by the development of methods to transfect embryos by electroporation. Immediate prospects to test the function of neural genes are based on the isolation of mutants by classical genetics and insertional mutagenesis, as well as by the disruption of gene function by morpholino antisense oligo-nucleotides. Coupled with high-speed video analysis of larval swimming, optophysiological methods offer the prospect to analyze at single-cell level the function of a CNS built on a vertebrate plan.
authors.affiliation: Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA. iam@dal.ca
authors.forename: Ian A
authors.initials: IA
authors.lastname: Meinertzhagen
authors.forename: Patrick
authors.initials: P
authors.lastname: Lemaire
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okamura
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D002938:Ciona intestinalis / Q000166:cytology / Q000196:embryology* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D023421:Models, Animal