[Optimized conditions for cholinesterase determination].
The impact of the buffer capacity and of buffer nature on the effective activity of enzymes was demonstrated by the example of blood choline esterase (CE). The enzyme activity, when determined in vitro, is inversely related with a size of the buffer capacity. The substitution of sodium for potassium entails a changed activity in the phosphate buffers, e.g. in case of CE--by 30%, which essentially surpasses the influence of pH within a range of 7.0-8.0. A possible mechanism of the observed phenomenon is under consideration.
Gerasimov
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D000328:Adult; D002802:Cholinesterases / Q000097:blood*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011199:Potentiometry
D002802:Cholinesterases