title: Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population.
abstract: A large body of evidence indicates that microorganisms are the primary causative agents of endodontic treatment failures. This study intended to assess the occurrence of nine putative endodontic pathogens in root-filled teeth associated with periradicular lesions in a South Korean population using a culture-independent molecular approach. Fourteen root-filled teeth with persistent periradicular diseases were selected for retreatment. After removal of the root canal filling, the canals were sampled, and a polymerase chain reaction assay using taxon-specific oligonucleotide primers was used for microbial detection. Bacteria were present in all cases, as revealed by amplification using ubiquitous 16S rDNA primers. The most frequently detected taxon was Enterococcus faecalis (64%), followed by Streptococcus spp. (21%) and Tannerella forsythensis (14%). The results of this study using a highly sensitive identification method are concurrent with those from other geographical locations using diverse identification methods in that E. faecalis is the main species found in cases of root-filled teeth associated with periradicular lesions.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
authors.forename: Isabela N
authors.initials: IN
authors.lastname: Rôças
authors.forename: Il-Young
authors.initials: IY
authors.lastname: Jung
authors.forename: Chan-Young
authors.initials: CY
authors.lastname: Lee
authors.forename: José F
authors.initials: JF
authors.lastname: Siqueira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification*; D001439:Bacteroides / Q000302:isolation & purification; D001442:Bacteroides Infections / Q000382:microbiology; D017931:DNA Primers; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000302:isolation & purification; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D010483:Periapical Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012390:Root Canal Therapy; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000145:classification; D017211:Treatment Failure
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S