Use of beta-glucuronidase reporter gene for gene expression analysis in turfgrasses.
The beta-glucuronidase (GUS) gene has been successfully used as a reporter gene in innumerable number of plant species. The functional GUS gene produces blue coloration in plants upon integration into the plant genome. Because of the ease it provides to analyze the gene expression (as no expensive equipment is needed), GUS gene is surely plant biotechnologist's first choice as a reporter gene. The turfgrass family contains the world's most economically important horticultural crops. There is a world-wide drive for genetic modification of grasses due to its huge economic importance. GUS gene can be transiently or stably expressed in grasses for the purpose of promoter analysis and to study tissue-specific and developmental gene expression. This paper summarizes the use of GUS gene for transient and stable expression studies in various turfgrass species.
Basu
Chhandak
C
Department of Plant Sciences, Woodward Hall, University of Rhode Island, Kingston, RI 02881, USA. chhandak@yahoo.com
Kausch
Albert P
AP
Chandlee
Joel M
JM
D020869:Gene Expression Profiling / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology*; D006109:Poaceae / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D005966:Glucuronidase