[Effect of exogenous basic fibroblast growth factor on proliferation and migration of endothelial cells of partial thickness scald in rats].
OBJECTIVE
To observe the proliferation and migration of endothelial cells after 30% total burn surface area (TBSA) of deep partial thickness scald, and the effect of basic fibroblast growth factor (bFGF) on angiogenesis during wound healing.


METHODS
A total of 133 male Wistar rats were divided randomly into normal control (n = 7), injured control group (n = 42), bFGF group (n = 42) and anti-c-fos group (n = 42). The apoptosis expression of fibroblasts was determined with in situ hybridization and the changes of proliferation cell nuclear antigen (PCNA), focal adhesion rinase(FAK), c-fos and extracellular signal-regulated kinase(ERK) proteins expression were detected with immunohistochemistry staining technique after 3 hours, 6 hours, 1 day, 3 days, 7 days, 14 days and 21 days of scald.


RESULTS
In injured control group and bFGF group, the proliferation rate of the vascular endothelial had evident changes 7 days and 14 days after scald; the expression of FAK was increased 14 days after scald. ERK proteins expression was different between injury control group and bFGF group at initial stage after scald. Stimulation of ERKs by bFGF led to up-regulation of c-fos and strong expression of FAK.


CONCLUSIONS
Exogenous bFGF extended the influence on wound healing process by ERK signaling pathway, affecting migration cascade of vascular endothelial cell. The oncogene proteins play an important role on accelerating angiogenesis during wound healing.
Cheng
Biao
B
Key Research Laboratory of Wound Repair, PLA 304th Hospital, Beijing, 100037, P. R. China.
Fu
Xiaobing
X
Sheng
Zhiyong
Z
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects
D018809:Proliferating Cell Nuclear Antigen; D016760:Proto-Oncogene Proteins c-fos; D016222:Fibroblast Growth Factor 2; D051416:Focal Adhesion Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases