Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural nucleotide.
We explore the adaptability at a protein-DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.
Simon
Matthew D
MD
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Shokat
Kevan M
KM
D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D009711:Nucleotides / Q000737:chemistry*; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009711:Nucleotides; D011993:Recombinant Fusion Proteins