title: Heat shock and cold shock in Deinococcus radiodurans.
abstract: On the basis of acquired thermotolerance and cryotolerance, the optimal heat shock and cold shock temperatures have been determined for Deinococcus radiodurans. A heat shock at 42 degrees C maximized survival at the lethal temperature of 52 degrees C and a cold shock at 20 degrees C maximized survival after repeated freeze-thawing. Enhanced survival from heat shock was found to be strongly dependent on growth stage, with its greatest effect shortly after phase. Increased synthesis of a total of 67 proteins during heat shock and 42 proteins during cold shock were observed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and autoradiography. Eight of the most highly induced heat shock proteins shown by 2D PAGE were identified by MALDI-MS as Hsp20, GroEL, DnaK, SodA, Csp, Protease I, and two proteins of unknown function.
authors.affiliation: Department of Geological & Environmental Sciences, Stanford University, Stanford, CA, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Airo
authors.forename: Suzanne L
authors.initials: SL
authors.lastname: Chan
authors.forename: Zak
authors.initials: Z
authors.lastname: Martinez
authors.forename: Manu O
authors.initials: MO
authors.lastname: Platt
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Trent
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D003080:Cold Temperature; D034301:Deinococcus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism; D018869:Heat-Shock Response; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins