[Inhibitory effect on mammary carcinoma cells induced by beta-ionone].
OBJECTIVE
To investigate the effect of cell proliferation induced by beta-ionone.


METHODS
Human mammary cancer cells (MCF-7) were treated with various beta-ionone concentrations (25, 50, 100 and 200 mumol/L), with a negative control. Methods such as curve of cell growth, cellular mitosis, the cell clone formatting and DNA synthesis were employed.


RESULTS
beta-ionone inhibited the cell proliferation, cellular mitosis, the cell clone formatting and DNA synthesis. The inhibitory frequency (IF) showed a dose-dependent response as the concentrations of beta-ionone increased. Its IC50 value was 104 mumol/L for MCF-7 cells. The inhibitory frequency (IF) from cellular mitosis of MCF-7 treated by beta-ionone were - 12.88% - 68.67% at 24 h and 20.79% - 87.79% at 48 h, from the cell clone formatting of MCF-7 treated by beta-ionone, were - 14.11% - 65.18% at 24 h and 6.58% - 72.48% at 48 h, from DNA synthesis, 16.75% - 65.33% at 24 h and 35.10% - 81.89% at 48 h.


CONCLUSIONS
beta-ionone could inhibit the cell growth of MCF-7 cells and the mechanisms needs to be further studied.
Liu
Jiaren
J
Public Health College, Harbin Medical University, Harbin 150001, China.
Li
Baixiang
B
Ma
Ruobo
R
Chen
Bingqing
B
D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000187:drug effects; D005260:Female; D006801:Humans; D045792:Norisoprenoids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D045792:Norisoprenoids; C008157:beta-ionone