A simple and rapid method for propagation and purification of the peripheral blood gammadeltaT cells.
The purpose of this study was to set up an approach for expansion of the peripheral blood gammadeltaT cells from normal subjects in order to explore the characteristics of gammadeltaT cells. Peripheral blood mononuclear cells (PBMNC) were separated from 5 - 10 ml peripheral blood and stimulated by the low molecular peptide derived from Mycobacterium tuberculosis (MTb-Ag), and expanded in rIL-2-containing medium. The relative amount of gammadeltaT cells were measured by anti TCR gammadelta-PE staining and flow cytometry. The Cytotoxicity were detected by gammadeltaT assay. The results showed that after stimulation and expansion for 10 days, gammadeltaT cells increased to 69.2% of the total PBMNC and demonstrated significant cytotoxicity against K562 cells. In conclusion, this is a simple, rapid and specific method for expansion of peripheral blood gammadeltaT cells in vitro.
Wang
Ke-Qiang
KQ
The Center Laboratory, The Affiliated Hospital of Taishan Medical College, Taian 271000, China. wkqsd@sohu.com
Hou
Yan-Qiang
YQ
Li
Yan
Y
D000942:Antigens, Bacterial / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000166:cytology*
D000942:Antigens, Bacterial; D016692:Receptors, Antigen, T-Cell, gamma-delta