title: Murine oviduct epithelial cell cytokine responses to Chlamydia muridarum infection include interleukin-12-p70 secretion.
abstract: Epithelial cells play an important role in host defense as sentinels for invading microbial pathogens. Chlamydia trachomatis is an intracellular bacterial pathogen that replicates in reproductive tract epithelium. Epithelial cells lining the reproductive tract likely play a key role in triggering inflammation and adaptive immunity during Chlamydia infections. For this report a murine oviduct epithelial cell line was derived in order to determine how epithelial cells influence innate and adaptive immune responses during Chlamydia infections. As expected, oviduct epithelial cells infected by Chlamydia muridarum produced a broad spectrum of chemokines, including CXCL16, and regulators of the acute-phase response, including interleukin-1alpha (IL-1alpha), IL-6, and tumor necrosis factor alpha. In addition, infected epithelial cells expressed cytokines that augment gamma interferon (IFN) production, including IFN-alpha/beta and IL-12-p70. To my knowledge this is the first report of a non-myeloid/lymphoid cell type making IL-12-p70 in response to an infection. Equally interesting, infected epithelial cells significantly upregulated transforming growth factor alpha precursor expression, suggesting a mechanism by which they might play a direct role in the pathological scarring seen as a consequence of Chlamydia infections. Data from these in vitro studies predict that infected oviduct epithelium contributes significantly to host innate and adaptive defenses but may also participate in the immunopathology seen with Chlamydia infections.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Indiana University School of Medicine, 545 Barnhill Dr. #435, Indianapolis, IN 46202, USA. raymjohn@iupui.edu
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000378:metabolism*; D023502:Chlamydia muridarum / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology; D005187:Fallopian Tubes / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism*; D051379:Mice; D021122:Protein Subunits / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016207:Cytokines; D021122:Protein Subunits; D012333:RNA, Messenger; D018664:Interleukin-12