Array assessment of phage-displayed peptide mimics of Human Immunodeficiency Virus type 1 gp41 immunodominant epitope: binding to antibodies of infected individuals.
An array of phage-displayed mimetic peptides representing 18 sequences from the HIV-1 gp41 immunodominant epitope was fabricated on a nitrocellulose membrane. It used to compare the antibody-binding affinity of the peptide and to monitor the immune response of four patients prior to and after the initiation of Highly Active Anti-Retroviral Therapy in parallel assays with several probes using near-infrared fluorescence detection. The proposed multiplexed approach is highly sensitive, consumes less sample and can be used to analyze the immune repertoires of virus-infected individuals.
Arnaud
Marie-Claire
MC
Biotechnologie, Biocatalyse, Biorégulation, CNRS UMR 6204, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France.
Gazarian
Tatyana
T
Palacios Rodriguez
Yadira
Y
Gazarian
Karlen
K
Sakanyan
Vehary
V
D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry; D023241:Antiretroviral Therapy, Highly Active; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000502:physiology*; D015497:HIV-1 / Q000378:metabolism*; D007074:Immunoglobulin G / Q000737:chemistry; D008969:Molecular Sequence Data; D019151:Peptide Library; D040081:Protein Array Analysis; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry
D000906:Antibodies; D000939:Epitopes; D015700:HIV Envelope Protein gp41; D007074:Immunoglobulin G; D019151:Peptide Library; D014764:Viral Proteins