Evaluation of an automated instrument for viability and concentration measurements of cryopreserved hematopoietic cells.
Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters. Using 33 thawed hematopoietic progenitor cell samples, automated Vi-Cell viability results were compared to those obtained using the standard trypan blue manual method. In addition, cell concentrations from these samples were compared with results from the Model Z2 Coulter Counter. Chinese Hamster Ovary cells were used for the evaluation of Vi-Cell linearity at the Beckman Coulter Cellular Analysis Development Center. Significant correlation was obtained when the two methods were compared for both cell concentration and percentage viability (P < .0001). The results of the linearity study indicated that the Vi-Cell is linear from approximately 5 x 10(4) to greater than 1 x 10(7) cells/mL. The Vi-Cell uses sample volumes as low as 0.5 mL; cell diameters may be 2 to 70 microns. The Vi-Cell automated instrument offers many significant advantages for cell analyses in today's busy laboratory environment.
Szabo
S E
SE
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland, USA. Steve.Szabo@Coulter.com
Monroe
S L
SL
Fiorino
S
S
Bitzan
J
J
Loper
K
K
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D019556:COS Cells; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D006224:Cricetinae; D015925:Cryopreservation; D005069:Evaluation Studies as Topic; D006412:Hematopoietic Stem Cells; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity