Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium.
Enterococcus faecalis and Enterococcus faecium infections are increasingly difficult to treat due to high levels of resistance to antibiotics. PlyV12, a bacteriophage lytic enzyme, was isolated and shown to effectively kill both E. faecalis and E. faecium (including vancomycin-resistant strains), as well as other human pathogens. We propose its development and use as an alternative therapeutic tool.
Yoong
Pauline
P
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave., New York, NY 10021, USA.
Schuch
Raymond
R
Nelson
Daniel
D
Fischetti
Vincent A
VA
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology*; D017124:Conserved Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D016984:Enterococcus faecium / Q000187:drug effects* / Q000254:growth & development; D008969:Molecular Sequence Data; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000737:chemistry / Q000494:pharmacology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013210:Staphylococcus / Q000187:drug effects / Q000254:growth & development; D014764:Viral Proteins / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D014764:Viral Proteins; D009238:N-Acetylmuramoyl-L-alanine Amidase