Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of taq polymerase by the disaccharide trehalose.
Spiess
Andrej-Nikolai
AN
Institute for Hormone and Fertility Research, Centre of Innovative Medicine, Falkenried 88, 20251 Hamburg, Germany.
Mueller
Nadine
N
Ivell
Richard
R
D000818:Animals; D001923:Brain Chemistry; D004247:DNA / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry*; D004795:Enzyme Stability; D006361:Heating; D051379:Mice; D018045:Receptors, Oxytocin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry*; D044366:Transition Temperature; D014199:Trehalose / Q000737:chemistry*
D004187:Disaccharides; D018045:Receptors, Oxytocin; D004247:DNA; D014199:Trehalose; D019914:Taq Polymerase