title: Localization of lipocaline-type prostaglandin D synthase in rat brain: immunoelectron microscopic study.
abstract: The distribution of lipocaline-type prostaglandin D synthase (L-PGDS) in rat brain was investigated by immunoelectron microscopy using a protein A-gold technique. In perivascular cells adjacent to the basement membrane of arterioles in the pia-arachnoid and of blood vessels in the subpial cortex, gold labeling was confined to the lumen of the dilated rough endoplasmic reticulum, and not found in the few lysosomes present in the cytoplasm. The results suggest that the perivascular cells secrete L-PGDS and seem not to degrade lipophilic molecules carried by L-PGDS. Moreover, gold particles representing the antigenic sites of L-PGDS were found in the Golgi apparatus, rough endoplasmic reticulum, vesicles, and nuclear envelope of arachnoid trabecular cells, arachnoid barrier cells, and arachnoid pia mater cells. The labeling was less detectable in the same organelles of choroid plexus epithelial cells, compared with leptomeningeal cells. In meningeal macrophages and parenchymal microglia, L-PGDS was detected in lysosomes, multivesicular bodies, and endocytic vesicles. The production of L-PGDS in perivascular cells is important to the various functions of this enzyme in brain parenchyma.
authors.affiliation: Department of Neurology, University of Yamanashi, Tamaho-cho, 409-3898 Yamanashi, Japan. nagat@yamanashi.ac.jp
authors.forename: Takamura
authors.initials: T
authors.lastname: Nagasaka
authors.forename: Masaki
authors.initials: M
authors.lastname: Hiraide
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Sugimoto
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shindo
authors.forename: Zenji
authors.initials: Z
authors.lastname: Shiozawa
authors.forename: Sadaki
authors.initials: S
authors.lastname: Yokota
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism*; D054834:Lipocalins; D008297:Male; D016253:Microscopy, Immunoelectron; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase