Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection.
We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.
Rosenke
Kyle
K
Deptartment of Microbiology, University of Idaho, Moscow, ID 83844-3052, USA.
Fortunato
Elizabeth A
EA
D001973:Bromodeoxyuridine / Q000378:metabolism*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics / Q000472:pathogenicity*; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D008856:Microscopy, Fluorescence; D014771:Virion / Q000378:metabolism
D004279:DNA, Viral; D004247:DNA; D001973:Bromodeoxyuridine