title: Development of transgenic chickens expressing enhanced green fluorescent protein.
abstract: In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.
authors.affiliation: Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Republic of Korea.
authors.forename: Mo Sun
authors.initials: MS
authors.lastname: Kwon
authors.forename: Bon Chul
authors.initials: BC
authors.lastname: Koo
authors.forename: Bok Ruyl
authors.initials: BR
authors.lastname: Choi
authors.forename: Hoon Taek
authors.initials: HT
authors.lastname: Lee
authors.forename: Young Hye
authors.initials: YH
authors.lastname: Kim
authors.forename: Wang-Shik
authors.initials: WS
authors.lastname: Ryu
authors.forename: Hosup
authors.initials: H
authors.lastname: Shim
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Teoan
authors.initials: T
authors.lastname: Kim
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D008845:Microinjections
chemical_list: D017931:DNA Primers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins