Agrobacterium-mediated transformation of Bangladeshi Indica rices.
Morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the Bangladeshi Indica rice cultivars BR26 and Binni with Agrobacterium tumefaciens strain LBA4404 carrying the super binary vector pTOK233. Acetosyringone (100 microM) in the medium during co-culture (25-28 degrees C) and selection on hygromycin B (50 mg l(-1)) were essential for efficient transformation. Stable integration and expression of beta-glucuronidase, neomycin phosphotransferase and hygromycin phosphotransferase genes in regenerated plants were confirmed by histochemical and fluorometric assays, ELISA and Southern analysis. Two to 3 copies of T-DNA were integrated into regenerated plants; transgene expression did not correlate with gene copy number. Mendelian segregation of transgenes occurred in T1 seed progeny.
Al-Forkan
Mahammad
M
Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
Power
J Brian
JB
Anthony
Paul
P
Lowe
Kenneth C
KC
Davey
Michael R
MR
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001459:Bangladesh; D018920:Coculture Techniques; D014644:Genetic Variation; D006801:Humans; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D030821:Plants, Genetically Modified; D014169:Transformation, Bacterial
D001426:Bacterial Proteins