title: Light and electron microscopical observations of the effects of high-density lipoprotein on growth of Plasmodium falciparum in vitro.
abstract: Human serum high-density lipoprotein (HDL) is necessary and sufficient for the short-term maintenance of Plasmodium falciparum in in vitro culture. However, at high concentrations it is toxic to the parasite. A heat-labile component is apparently responsible for the stage-specific toxicity to parasites within infected erythrocytes 12-42 h after invasion, i.e. during trophozoite maturation. The effects of HDL on parasite metabolism (as determined by nucleic acid synthesis) are evident at about 30 h after invasion. Parasites treated with HDL show gross abnormalities by light and electron microscopy.
authors.affiliation: Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, UK. heather.imrie@ceid.ox.ac.uk
authors.forename: H
authors.initials: H
authors.lastname: Imrie
authors.forename: D J P
authors.initials: DJ
authors.lastname: Ferguson
authors.forename: M
authors.initials: M
authors.lastname: Carter
authors.forename: J
authors.initials: J
authors.lastname: Drain
authors.forename: A
authors.initials: A
authors.lastname: Schiflett
authors.forename: S L
authors.initials: SL
authors.lastname: Hajduk
authors.forename: K P
authors.initials: KP
authors.lastname: Day
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000469:parasitology / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D019271:Hypoxanthine / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology; D016778:Malaria, Falciparum / Q000469:parasitology*; D008854:Microscopy, Electron; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D019271:Hypoxanthine