title: 2'-Functionalized nucleic acids as structural tools in molecular biology.
abstract: Modified oligonucleotides bearing 2'-reactive groups or 2'-conjugated molecules have found wide application as structural tools in molecular biology. Of principal interest has been the use of 2'-reactive oligonucleotides for cross-linking with biomolecules and of 2'-conjugated oligonucleotides in hybridization assays. In this review we compare a range of electrophilic, nucleophilic and photoreactive groups for cross-linking and conjugation studies.
authors.affiliation: Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie gory, Moscow 119992, Russia.
authors.forename: Timofei S
authors.initials: TS
authors.lastname: Zatsepin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gait
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Oretskaya
mesh_terms: D003432:Cross-Linking Reagents / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D015342:DNA Probes; D009841:Oligonucleotides; D012313:RNA