title: Expression of P2X4 receptor in rat C6 glioma by tumor-associated macrophages and activated microglia.
abstract: Here we report an immunohistochemical analysis of P2X4 receptor (P2X4R), an ATP-gated ion channel, expression in rat C6 glioma model. Striking P2X4R expression was detected in compact and infiltrative tumor growth areas. Expression of P2X4R by perivascular cells was observed in normal control brain and in brain areas not affected by tumor growth. Double-immunolabeling revealed that P2X4R was co-expressed by ED1+, AIF-1+, and EMAP II+ tumor-infiltrating microglia/macrophages; whereas on GFAP+ and nestin+ astrocytes P2X4R was hardly seen. Our results indicate that P2X4R expression defines a distinct subset of tumor-associated macrophages and activated microglia in glioma.
authors.affiliation: Institute of Brain Research, University of Tuebingen, Calwer Str.3, D-72076 Tuebingen, Germany. lianghao.guo@uni-tuebingen.de
authors.forename: Liang-Hao
authors.initials: LH
authors.lastname: Guo
authors.forename: Katrin
authors.initials: K
authors.lastname: Trautmann
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Schluesener
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D005910:Glioma / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D017628:Microglia / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D058848:Receptors, Purinergic P2X4
chemical_list: C548228:P2rx4 protein, rat; D018048:Receptors, Purinergic P2; D058848:Receptors, Purinergic P2X4