[DNA damages and changes of certain hepatic biochemical indexes in rats exposed to vinyl chloride monomer].
OBJECTIVE
To investigate DNA damages of liver cells and lymphocytes of rats exposed to vinyl chloride monomer(VCM), to examine the changes of hepatic function, and certain biochemical indexes.


METHODS
DNA damages were detected by single cell gel electrophoresis (comet assay). The activities of metabolizing enzymes were measured by spectrophotography, and hepatic function was determined by biochemical analyser.


RESULTS
The incidence rates of comet cells in the experimental groups were significantly higher than those of the controls; and the rates of comet cells between liver cells and lymphocytes among the groups were closely correlated.


CONCLUSIONS
VCM could induce DNA damage of liver cells and lymphocytes, where was a dose-effect/response relationship.
Zhu
Shoumin
S
School of Public Health of Fudan University, Shanghai 200032, China.
Wang
Aihong
A
Liu
Guiming
G
Sun
Zuyue
Z
D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014752:Vinyl Chloride / Q000633:toxicity*; D014753:Vinyl Compounds / Q000633:toxicity
D014753:Vinyl Compounds; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase; D014752:Vinyl Chloride