Light and electron microscopical observations of the effects of high-density lipoprotein on growth of Plasmodium falciparum in vitro.
Human serum high-density lipoprotein (HDL) is necessary and sufficient for the short-term maintenance of Plasmodium falciparum in in vitro culture. However, at high concentrations it is toxic to the parasite. A heat-labile component is apparently responsible for the stage-specific toxicity to parasites within infected erythrocytes 12-42 h after invasion, i.e. during trophozoite maturation. The effects of HDL on parasite metabolism (as determined by nucleic acid synthesis) are evident at about 30 h after invasion. Parasites treated with HDL show gross abnormalities by light and electron microscopy.
Imrie
H
H
Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, UK. heather.imrie@ceid.ox.ac.uk
Ferguson
D J P
DJ
Carter
M
M
Drain
J
J
Schiflett
A
A
Hajduk
S L
SL
Day
K P
KP
D000818:Animals; D004912:Erythrocytes / Q000469:parasitology / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D019271:Hypoxanthine / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology; D016778:Malaria, Falciparum / Q000469:parasitology*; D008854:Microscopy, Electron; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D019271:Hypoxanthine