title: Mutagenic insertion and chromosome engineering resource (MICER).
abstract: Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Adams
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Biggs
authors.forename: Tony
authors.initials: T
authors.lastname: Cox
authors.forename: Rob
authors.initials: R
authors.lastname: Davies
authors.forename: Louise
authors.initials: L
authors.lastname: van der Weyden
authors.forename: Jos
authors.initials: J
authors.lastname: Jonkers
authors.forename: James
authors.initials: J
authors.lastname: Smith
authors.forename: Bob
authors.initials: B
authors.lastname: Plumb
authors.forename: Ruth
authors.initials: R
authors.lastname: Taylor
authors.forename: Ichiko
authors.initials: I
authors.lastname: Nishijima
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Yu
authors.forename: Jane
authors.initials: J
authors.lastname: Rogers
authors.forename: Allan
authors.initials: A
authors.lastname: Bradley
mesh_terms: D000818:Animals; D015723:Gene Library; D020868:Gene Silencing; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D011995:Recombination, Genetic; D017384:Sequence Deletion