title: Suppression of myosin-induced and adoptively transferred myocarditis by prior treatment with complete Freund's adjuvant.
abstract: BACKGROUND
  We evaluated the effect of pretreatment with complete Freund's adjuvant (CFA) on experimentally induced myocarditis concomitant with the assessment of antigen-specific properties of disease-triggering lymphocytes.
  
  
  RESULTS
  Rats pretreated with CFA a week prior to myosin immunization developed a significantly attenuated myocardial inflammation as compared to control-treated animals. Furthermore, prior administration of CFA virtually abolished histological evidence of myocarditis induced by transfer of antimyosin lymphocytes. CFA administered subcutaneously prior to myosin immunization resulted in a significant reduction in lymph node cell reactivity to myosin. Assessment of cultured medium from lymphocytes obtained from CFA-pretreated myosin-immunized rats revealed reduced levels of interferon gamma but an increased production of IL-10, suggesting an induction of a Thl to Th2 switch.
  
  
  CONCLUSIONS
  Thus, CFA treatment suppressed both myosin-induced as well as adoptively transferred myocarditis concomitant with induction of antigen-specific unresponsiveness to myosin and skewing of the immune response in favor of a Th2-dominated profile.
authors.affiliation: Department of Cardiology, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel. jacobg@post.tau.ac.il
authors.forename: Jacob
authors.initials: J
authors.lastname: George
authors.forename: Arnon
authors.initials: A
authors.lastname: Adler
authors.forename: Iris
authors.initials: I
authors.lastname: Barshack
authors.forename: Gad
authors.initials: G
authors.lastname: Keren
authors.forename: Arie
authors.initials: A
authors.lastname: Roth
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D019264:Adoptive Transfer; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D009205:Myocarditis / Q000473:pathology / Q000517:prevention & control*; D009218:Myosins / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000276:Adjuvants, Immunologic; D016753:Interleukin-10; D007371:Interferon-gamma; D005620:Freund's Adjuvant; D009218:Myosins