Acteoside protects endothelial cells against free radical-induced oxidative stress.
The protective effect of acteoside against membrane lipid oxidation and free radical-mediated impairment of endothelial function was investigated. Results showed that iron-mediated oxidative modification of the cell membrane in cultured bovine pulmonary endothelial cells (PAECs) was significantly attenuated by acteoside as measured by thiobarbituric acid-reactive substances (TBARS). Fenton's reagent (H2O2/Fe2+) was used to generate hydroxyl radicals (*OH) and induce oxidative stress. Acteoside not only effectively minimized the loss of cell viability induced by hydroxyl radicals in cultured endothelial cells but also countered the free radical-induced destruction of the endothelium-dependent relaxation to acetylcholine in rat aorta. Furthermore, acteoside showed a dose-dependent scavenging effect of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals and appeared to be the most efficient in comparison with the four reference compounds (alpha-tocopherol, vitamin C, probucol and resveratrol). These data suggested that acteoside protects the cell from oxidative stress and that scavenging of free radicals could be a key mechanism contributing to the cytoprotective effect of acteoside.
Chiou
Wen Fei
WF
National Research Institute of Chinese Medicine, NO. 155-1, SEC. 2, Li-Nung Street, Shipai, Taipei, Taiwan. wfchiou@cma23@.nricm.edu.tw
Lin
Lie Chwen
LC
Chen
Chieh Fu
CF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D001713:Biphenyl Compounds; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006834:Hydrazines; D066298:In Vitro Techniques; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D018384:Oxidative Stress; D010636:Phenols / Q000494:pharmacology*; D010851:Picrates; D011651:Pulmonary Artery / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects; D059808:Polyphenols
D000975:Antioxidants; D001713:Biphenyl Compounds; D005960:Glucosides; D006834:Hydrazines; D008563:Membrane Lipids; D010636:Phenols; D010851:Picrates; D017392:Thiobarbituric Acid Reactive Substances; C058956:acteoside; D059808:Polyphenols; C004931:1,1-diphenyl-2-picrylhydrazyl