Analysis of the polymorphisms in eotaxin gene family and their association with asthma, IgE, and eosinophil.
The eotaxin gene family (eotaxin, eotaxin-2, and eotaxin-3) has been implicated in the recruitment of eosinophils, basophiles, and Th2 lymphocytes that is a central aspect of allergic diseases such as asthma. To determine whether the single nucleotide polymorphisms (SNPs) of eotaxin gene family are associated with susceptibility to asthma, we scanned 225 asthma patients and 294 non-asthmatic controls using the direct sequencing method. We further investigated the relationships among each SNP, eosinophils, and serum total IgE levels in asthma patients. Eleven SNPs were identified in the eotaxin gene family. We found that EoB179T > C (P = 0.0001), EoB275C > T (P = 0.018) of the eotaxin-2 and EoA2497T > G (P = 0.003) of the eotaxin-3 were significantly associated with the susceptibility of asthma. Furthermore, our data demonstrated for the first time that EoA2497T > G (P = 0.005) is related to serum total IgE level while EoA77C > T (P = 0.035) and EoA2497T > G (P = 0.033) are related to the peripheral blood eosinophil counts in asthma. Our results suggest that the polymorphisms of the eotaxin gene family are associated with the susceptibility of asthma and Eotaxin-3 might play the critical role for the recruitment of eosinophils and the maintenance of IgE levels.
Chae
Soo-Cheon
SC
Genome Research Center for Immune Disorders, Wonkwang University School of Medicine, Iksan, Chonbuk 570-749, Republic of Korea.
Lee
Yong-Chul
YC
Park
Young-Ran
YR
Shin
Jung-Sup
JS
Song
Ju-Hee
JH
Oh
Gyung-Jae
GJ
Hong
Seong-Tshool
ST
Pae
Hyun-Ock
HO
Choi
Byung-Min
BM
Chung
Hun-Taeg
HT
D000328:Adult; D001249:Asthma / Q000097:blood / Q000453:epidemiology* / Q000235:genetics*; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007723:Korea / Q000453:epidemiology; D007958:Leukocyte Count; D008297:Male; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013223:Statistics as Topic
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D007073:Immunoglobulin E