title: The effect of phytase enzyme and level on nutrient extraction by broilers.
abstract: Three experimental phytase enzyme preparations derived from the same Escherichia coli gene but produced in Saccharomyces cerevisiae (A), Pichia pastoris (B), and Pseudomonas fluorescens (C) were compared with a commercial enzyme preparation by addition to wheat-soybean meal diets fed to broiler chicks. A positive control diet contained sufficient available phosphorus for normal broiler growth and a negative control diet was phosphorus deficient. The 4 enzymes were added to the negative control diet at 3 levels each (150, 450, and 1,250 U/kg), and all diets were pelleted above 80 degrees C. Broiler chicks were fed experimental diets from 4 to 21 d. Chick performance and nutrient digestibility showed that the pel leting process inactivated enzymes A and C and the commercial enzyme. When added to the negative control diet, enzyme B had positive effects on broiler performance and calcium and phosphorus digestibility, and increasing levels of enzyme had greater positive effects. Enzyme B also increased the AME and protein digestibility over those of either control diet. These results suggest that enzyme B was not inactivated by pelleting above 80 degrees C, whereas the other enzymes were.
authors.affiliation: Crops and Livestock Research Centre, Charlottetown, Prince Edward Island, Canada. silversidesf@agr.gc.ca
authors.forename: F G
authors.initials: FG
authors.lastname: Silversides
authors.forename: T A
authors.initials: TA
authors.lastname: Scott
authors.forename: M R
authors.initials: MR
authors.lastname: Bedford
mesh_terms: D010832:6-Phytase / Q000008:administration & dosage* / Q000235:genetics; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002136:Calcium, Dietary / Q000008:administration & dosage; D002645:Chickens / Q000502:physiology*; D004032:Diet; D004063:Digestion; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D016226:Phosphorus, Dietary / Q000008:administration & dosage / Q000378:metabolism; D010843:Pichia / Q000201:enzymology; D011551:Pseudomonas fluorescens / Q000201:enzymology; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013025:Glycine max; D014908:Triticum
chemical_list: D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D011994:Recombinant Proteins; D010832:6-Phytase