Identification and characterization of two contiguous operons required for aerobactin transport and biosynthesis in Vibrio mimicus.
In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V. mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron-regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V. mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin-mediated iron acquisition system in V. mimicus is unique in that the aerobactin operon (iucABCD iutA ) is contiguous to the operon (matCDB ) encoding components of an ATP-binding cassette transport system for ferric aerobactin. This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae.
Moon
Yong-Hwa
YH
Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Tanabe
Tomotaka
T
Funahashi
Tatsuya
T
Shiuchi
Kei-ichi
K
Nakao
Hiroshi
H
Yamamoto
Shigeo
S
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001693:Biological Transport, Active / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D023061:Gene Order; D005798:Genes, Bacterial; D015698:Genomic Library; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D033902:Periplasmic Binding Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017262:Siderophores / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D044164:Vibrio mimicus / Q000235:genetics* / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C066629:FhuC protein, E coli; D006877:Hydroxamic Acids; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; D017262:Siderophores; C034506:aerobactin receptor; C069968:fhuB protein, E coli; C069969:fhuD protein, E coli; C031819:aerobactin; D007501:Iron