title: Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7.
abstract: Cytochrome P450 from thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (P450st) has been expressed in Escherichia coli and purified at high homogeneity. P450st was crystallized in an orthorhombic system with the space group P2(1)2(1)2(1) and cell dimensions of a=53.6 A, b=55.1 A, and c=130.9 A, and the structure was determined at a 3.0 A resolution. The final R-factor was 0.194 (Rfree=0.235). Structural comparison with cytochrome P450 from S. solfataricus (CYP119) suggests that the region composed of the F to G helices and the Cl- binding site is responsible for the affinity for a ligand coordinating heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide (DDAB) film on a plastic formed carbon (PFC) electrode has also been demonstrated. A quasi-reversible redox response has been observed even at elevated temperatures of up to 80 degrees C.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oku
authors.forename: Akashi
authors.initials: A
authors.lastname: Ohtaki
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Kamitori
authors.forename: Nobuhumi
authors.initials: N
authors.lastname: Nakamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohno
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawarabayasi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D004563:Electrochemistry; D006418:Heme / Q000737:chemistry; D017434:Protein Structure, Tertiary; D016944:Sulfolobus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D006418:Heme; D003577:Cytochrome P-450 Enzyme System