Cd2+ -promoted mitochondrial permeability transition: a comparison with other heavy metals.
We compared action of Cd(2+), Hg(2+), and Cu(2+) on isolated rat liver mitochondria in the absence of added Ca(2+) and P(i). The heavy-metal ions produced dose-dependently: (1) enhanced membrane permeabilization manifested in mitochondrial swelling and activation of basal respiration, (2) inhibition of uncoupler-stimulated respiration, and (3) membrane potential dissipation. Among the metals, Cu(2+) exhibited maximal stimulatory effect on basal respiration and minimal inhibitory action on DNP-uncoupled respiration whilst Cd(2+) promoted the strongest depression of uncoupled respiration and the largest swelling in NH(4)NO(3) medium. Dithiothreitol induced a basal respiration release if added after high [Cd(2+)] and [Hg(2+)], and the stimulation was CsA-insensitive.
Belyaeva
Elena A
EA
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St.-Petersburg, Russia. belyaeva@iephb.ru
Glazunov
Vadim V
VV
Korotkov
Sergey M
SM
D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D002588:Cesium Radioisotopes / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008628:Mercury / Q000494:pharmacology*; D019216:Metals, Heavy / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects*; D010101:Oxygen Consumption; D051381:Rats; D013997:Time Factors
D002588:Cesium Radioisotopes; D004791:Enzyme Inhibitors; D019216:Metals, Heavy; D002104:Cadmium; D003300:Copper; D016572:Cyclosporine; D008628:Mercury; D004229:Dithiothreitol