Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels.
A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3',5'-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (< or =5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.
Donaldson
Lara
L
Department of Molecular and Cell Biology, University of Cape Town, Private Bag Rondebosch 7701, South Africa.
Ludidi
Ndiko
N
Knight
Marc R
MR
Gehring
Chris
C
Denby
Katherine
K
D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D007700:Kinetics; D009994:Osmolar Concentration; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013012:Sorbitol / Q000494:pharmacology
D012965:Sodium Chloride; D013012:Sorbitol; D006152:Cyclic GMP; D002118:Calcium