Determination of (2,3-epoxypropyl)trimethylammonium chloride, (3-chloro-2-hydroxypropyl)trimethylammonium chloride, and (2,3-dihydroxypropyl)trimethylammonium chloride by capillary electrophoresis.
Capillary electrophoresis (CE) is a powerful technique to determine (2,3-epoxypropyl)trimethylammonium chloride, (3-chloro-2-hydroxypropyl)trimethylammonium chloride and their hydrolysis product (2,3-dihydroxypropyl)trimethylammonium chloride in complex matrices. The separation was performed in a 180 mM copper(II) sulphate-4 mM formic acid buffer at pH 3. The UV inactive target compounds were detected by indirect UV detection at 215 nm with a reference wavelength of 300 nm--copper(II) being the chromophor. The results were compared to data acquired by ion-pair HPLC with perchlorate as ion pair former in a 1 M aqueous sodium perchlorate solution in 0.2 mM phosphoric acid (pH 3.4) on a RP 18 column and refractive index detection as reference method. Results of both methods are in good agreement.
Goclik
Vera
V
TU Braunschweig, Institut für Lebensmittelchemie, Schleinitzstrasse 20, D-38106 Braunschweig, Germany.
Stach
Susanne
S
Mischnick
Petra
P
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D004852:Epoxy Compounds / Q000032:analysis*; D020005:Propanols / Q000032:analysis*; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D004852:Epoxy Compounds; D020005:Propanols; D000644:Quaternary Ammonium Compounds; C045006:glycidyl trimethylammonium; C496948:(3-chloro-2-hydroxypropyl)trimethylammonium chloride