Wound-induced RNaseLE expression is jasmonate and systemin independent and occurs only locally in tomato (Lycopersicon esculentum cv. Lukullus).
Tomato RNaseLE is induced by phosphate deficiency and wounding and may play a role in macromolecular recycling as well as wound healing. Here, we analyzed the role of jasmonate and systemin in the wound-induced RNaseLE activation. The rapid expression of RNaseLE upon wounding of leaves leading to maximal RNase activity within 10 h, appeared only locally. Jasmonic acid (JA) or its molecular mimic ethyl indanoyl isoleucine conjugate did not induce RNaseLE expression. Correspondingly, RNaseLE was expressed upon wounding of 35S::allene oxide cyclase antisense plants known to be JA deficient. RNaseLE was not expressed upon systemin treatment, but was locally expressed in the spr1 mutant which is affected in systemin perception. In tomato plants carrying a PromLE::uidA construct, GUS activity could be detected upon wounding, but not following treatment with JA or systemin. The data indicate a locally acting wound-inducible systemin- and JA-independent signaling pathway for RNaseLE expression.
Gross
Nadine
N
Biocenter, Martin Luther University Halle-Wittenberg, Weinbergweg 22, D-06120 Halle, Germany.
Wasternack
Claus
C
Köck
Margret
M
D003517:Cyclopentanes / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018551:Solanum lycopersicum / Q000201:enzymology*; D054883:Oxylipins; D010455:Peptides / Q000378:metabolism; D010935:Plant Diseases; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics*
D003517:Cyclopentanes; D054883:Oxylipins; D010455:Peptides; D010940:Plant Proteins; C073704:systemin; C011006:jasmonic acid; C488994:RNaseLE protein, Tomato; D012260:Ribonucleases