title: Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins.
abstract: Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.
authors.affiliation: Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, IL 60616, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Mathew
authors.forename: Ahmed
authors.initials: A
authors.lastname: Mirza
authors.forename: Nick
authors.initials: N
authors.lastname: Menhart
mesh_terms: D001665:Binding Sites; D002850:Chromatography, Gel / Q000379:methods*; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry; D045304:Cytochromes c / Q000032:analysis / Q000737:chemistry; D019281:Dimerization; D046911:Macromolecular Substances; D010316:Particle Size; D010958:Plasminogen / Q000032:analysis / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D045424:Complex Mixtures; D046911:Macromolecular Substances; D011506:Proteins; D010958:Plasminogen; D045304:Cytochromes c