Heparan sulphate identified on human erythrocytes: a Plasmodium falciparum receptor.
HS (heparan sulphate) has hitherto not been found on human red blood cells (RBCs, erythrocytes). However, malarial-parasite (Plasmodium falciparum)-infected RBCs adhere to uninfected RBCs via HS-like receptors. In the present paper we demonstrate that human RBCs carry epitopes for an anti-HS antibody. Glycans isolated from RBC membranes reacted to HS-specific degradations and adhered to an HS-binding malaria antigen. Additionally, an HS core protein was identified. This suggests that HS is present on human RBCs.
Vogt
Anna M
AM
Microbiology and Tumorbiology Center, Karolinska Institutet, Box 280, SE-171 77 Stockholm, Sweden. Anna.Vogt@mtc.ki.se
Winter
Gerhard
G
Wahlgren
Mats
M
Spillmann
Dorothe
D
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002478:Cells, Cultured; D000939:Epitopes / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D006497:Heparan Sulfate / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000378:metabolism; D010963:Plasmodium falciparum / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D006497:Heparan Sulfate; D010455:Peptides; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum