Immunohistochemical detection of C-100 hepatitis C virus antigen in formaldehyde-fixed paraffin-embedded liver tissue. Correlation with serum, tissue and in situ RT-PCR results.
We localized HCV C-100 protein in liver biopsies of 15 patients with chronic hepatitis C using immunohistochemistry. The results were compared to serum, tissue extract analysis of HCV RNA and in situ RT-PCR described in a previous study. HCV was detected in 80% of the sera tested, in 40% of the tissue extracts and in 80% and 60% of the tissue sections tested by immunohistochemistry and in situ RT-PCR respectively. Compared to the serum positive cases, 83% and 67% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR and 41% were positive with tissue extract detection. Compared to the tissue extract positive cases, 25% and 50% of the cases were respectively positive with immunohistochemistry and in situ RT-PCR. Finally, 75% of the cases positive by immunohistochemistry were also positive by in situ RT-PCR. These results underline the complementarity of the different methods for the precise diagnosis of hepatitis C.
Benkoël
L
L
INSERM U 559, Physiopathologie des Cellules Epithéliales, Faculté de Médecine, 27, boulevard Jean Moulin, 13385 Marseille Cedex 05, France. liliane.benkoel@medecine.univ-mrs.fr
Biagini
P
P
Dodero
F
F
De Lamballerie
X
X
De Micco
P
P
Chamlian
A
A
D005557:Formaldehyde / Q000737:chemistry*; D018936:Hepatitis C Antigens / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure / Q000821:virology*; D015233:Models, Statistical; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis / Q000097:blood / Q000235:genetics*; D015203:Reproducibility of Results; D016707:Tissue Fixation
D018936:Hepatitis C Antigens; D012367:RNA, Viral; D005557:Formaldehyde