Expression of retinoic acid-inducible gene-I in vascular smooth muscle cells stimulated with interferon-gamma.
Vascular smooth muscle cells (SMC) play an important role in atherogenesis and vasospasm. Interferon-gamma (IFN-gamma) is a potent cytokine that regulates immune and inflammatory responses by inducing multiple genes in many types of cells including SMC. Retinoic acid-inducible gene-I (RIG-I) is a putative RNA helicase, but its physiological function is not known. RIG-I is induced in leukemic cells by retinoic acid or in endothelial cells by lipopolysaccharide. We have studied the expression of RIG-I in cultured SMC from human umbilical artery. IFN-gamma stimulated SMC to express RIG-I mRNA and protein in concentration- and time-dependent manners. Immunohistochemical analysis revealed the expression of RIG-I in SMC in vivo. We conclude that RIG-I may play some pathophysiological role in immune and inflammatory reactions in SMC.
Imaizumi
Tadaatsu
T
Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. timaizum@cc.hirosaki-u.ac.jp
Yagihashi
Norito
N
Hatakeyama
Masaharu
M
Yamashita
Koji
K
Ishikawa
Akira
A
Taima
Kageaki
K
Yoshida
Hidemi
H
Inoue
Ituro
I
Fujita
Takashi
T
Yagihashi
Soroku
S
Satoh
Kei
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases