Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method.
We, for the first time, analyzed the binding motifs of immunoglobulin G (IgG) in the cerebrospinal fluid (CSF) of human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients with a phage library displaying 12-mer random peptides. As a result, the sequences highly homologous to HTLV-I gp46 192-199, 237-243 and 255-261 were the common linear epitopes of high affinity- IgG exclusively detected in both CSF and sera of the patients. These IgG responses were confirmed with corresponding HTLV-I peptides and serum antibody titers significantly correlated with disease severity or duration. Gp46 237-243 has not been detected in previous enzyme-linked immunosorbent assay (ELISA) studies using bound longer peptides, suggesting the usefulness of the phage display method.
Fujimori
J
J
Department of Neurology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. fujimori@em.neurol.med.tohoku.ac.jp
Nakashima
I
I
Fujihara
K
K
Feng
J
J
Yamamoto
M
M
Yamamoto
N
N
Begum
N
N
Sagara
Y
Y
Shiraki
H
H
Shiga
Y
Y
Onodera
J
J
Sato
S
S
Takase
S
S
Asano
M
M
Endo
M
M
Itoyama
Y
Y
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D015481:HTLV-I Antibodies / Q000134:cerebrospinal fluid* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000276:immunology*; D019151:Peptide Library; D017385:Sequence Homology
D000939:Epitopes; D015481:HTLV-I Antibodies; D007074:Immunoglobulin G; D019151:Peptide Library