[The induction of differentiation into dendritic cells from HL-60 cells by calcium ionophore].
OBJECTIVE
To explore whether the promyelocytic leukemia cell line HL-60 may differentiate into activated dendritic cells (DCs) by A23187, a calcium ionophore.


METHODS
The HL-60 cells were cultured in common medium alone or with various concentrations of A23187 (25-1 600 microg/L) and rhGM-CSF (100 microg/L). After culture for 24-96 hours, the cellular morphological change was observed under light microscope and electron microscope. Surface makers on treated HL-60 cells were analyzed by flow cytometry. The proliferation of allogeneic human T cells was detected by MTT colorimetry.


RESULTS
Under the condition of a suitable dose (200 microg/L) of A23187 treatment of HL-60 cells for 24 hours, the expression of CD83 molecule, a characteristic marker on DCs, was highest. The typical dendritic outgrowth appeared at a time when HL-60 cells were treated with A23187 for 72 hours. However, when HL-60 cells were treated with A23187 for 96 hours, the expressions of CD80 (B7.1), CD86 (B7.2), MHC-class II molecule and CD54 on HL-60 cells reached peak, and marked activation of allogeneic T cells occurred.


CONCLUSIONS
Calcium ionophore A23187 can induce the HL-60 cells to differentiate into activated DCs-like cells.
Wu
Jun
J
Institute of Tumor Molecular Biology, General Hospital of Guangzhou Command, Guangzhou 510010, China. Junwuwang@hotmail.com
Yang
Tai-cheng
TC
Xian
Jiang
J
Wang
Jie
J
Chen
Zheng-liang
ZL
Fu
Ning
N
D015703:Antigens, CD / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D000001:Calcimycin / Q000494:pharmacology*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D000097802:CD83 Antigen
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D000001:Calcimycin; D007136:Immunoglobulins; D018799:Intercellular Adhesion Molecule-1; D007476:Ionophores; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C497964:CD86 protein, human