Can GH induce chromosome breaks or microsatellite instability in GH-deficient children?
The role of GH in carcinogenesis is unclear. We studied the effect of recombinant human GH (rhGH) in vitro on chromosomal and genomic instability. Thirteen children, aged 9.57 +/- 1.08 yr (mean +/- SEM), with complete (no.=5) or partial (no.=8) GH deficiency were evaluated before and during GH treatment. We examined the incidence of chromosome and chromatid breaks, and microsatellite instability after in vitro addition of two different doses of rhGH to peripheral blood lymphocytes obtained from the patients. No differences were observed between the samples of GH-deficient children before and after GH therapy as regards the percentage of chromosome and chromatids breaks, and in microsatellite instability. Our data show that in vitro addition of rhGH does not induce chromosomal and/or genomic instability in cultured lymphocytes.
Olivieri
C
C
Institute of Biology and Medical Genetics, University of Pavia, IRCCS San Matteo, Pavia, Italy.
Danesino
C
C
Scappaticci
S
S
Bozzola
M
M
D002478:Cells, Cultured; D002648:Child; D019457:Chromosome Breakage; D004247:DNA / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019382:Human Growth Hormone / Q000009:adverse effects* / Q000172:deficiency* / Q000627:therapeutic use; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D018895:Microsatellite Repeats / Q000187:drug effects*; D016133:Polymerase Chain Reaction
D019382:Human Growth Hormone; D004247:DNA