Diagnosis of intraocular lymphoma.
OBJECTIVE
To review current techniques for the diagnosis of intraocular lymphoma.


METHODS
Literature review.


RESULTS
There are a limited number of small case series and case reports reporting the diagnosis of intraocular lymphoma. Limitations in the ability to diagnose lymphoma through cytologic examination of the vitreous has led to investigations into immunohistochemistry either manually or by automated cytofluorography, intraocular cytokine determinations, and polymerase chain reaction of the vitreous or tissue lymphoid cells to detect gene rearrangements in the IgH, bcl-2, or T-cell receptor gamma gene. Data regarding diagnostic efficiency of the various testing strategies is limited by small patient numbers. Careful handling of the vitreous specimen and adequate laboratory and pathological support is critical.


CONCLUSIONS
Despite a proliferation of new techniques for the diagnosis of intraocular lymphoma, none is clearly superior. Use of multiple testing strategies may improve diagnostic yield.
Davis
Janet L
JL
University of Miami School of Medicine, Bascom Palmer Eye Institute, Miami, FL 33136, USA. Jdavis@med.miami.edu
D003941:Diagnostic Techniques, Ophthalmological; D005134:Eye Neoplasms / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000175:diagnosis*; D014822:Vitreous Body / Q000473:pathology*