title: ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum: divergent patterns and stage-dependent regulation during the natural menstrual cycle.
abstract: Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalloproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P<0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P<0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P<0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.
authors.affiliation: Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Ave, Beaverton, Oregon 97006, USA.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Young
authors.forename: Barton
authors.initials: B
authors.lastname: Tumlinson
authors.forename: Richard L
authors.initials: RL
authors.lastname: Stouffer
mesh_terms: D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D000818:Animals; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D019483:Disintegrins / Q000235:genetics / Q000378:metabolism*; D004958:Estradiol / Q000097:blood; D005260:Female; D006727:Hormone Antagonists / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008253:Macaca mulatta; D008597:Menstrual Cycle / Q000502:physiology*; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000378:metabolism*
chemical_list: D019483:Disintegrins; D006727:Hormone Antagonists; D009842:Oligopeptides; D012333:RNA, Messenger; D019717:Tissue Inhibitor of Metalloproteinase-3; D013196:Dihydrotestosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; C057022:iturelix; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; D008666:Metalloendopeptidases; C009954:trilostane