c-Myb protein interacts with Rcd-1, a component of the CCR4 transcription mediator complex.
Transcriptional initiation of eukaryotic genes depends on the cooperative interaction of various transcription factors. Using the yeast two-hybrid assay, we have identified the murine Rcd-1 protein as a cofactor of the c-myb proto-oncogene product. Rcd-1 is evolutionarily conserved among many species, and moreover the yeast homologue CAF40 is part of the carbon catabolite repressor protein transcriptional mediator thought to be involved in the negative regulation of genes transcribed by RNA polymerase II. Rcd-1 is located mainly in the nucleus, and it interacts with c-Myb both in vitro and in vivo. The activation of the myeloid c-myb-specific mim-1 promoter is repressed by Rcd-1. Interestingly, rcd-1 is an erythropoietin regulated gene, which also represses the action of the AP-1 transcription factor on its target genes.
Haas
Martin
M
Institute for Biophysical Chemistry, Hannover Medical School, Carl-Neuberg-Strasse 30625 Hannover, Germany.
Siegert
Michaela
M
Schürmann
André
A
Sodeik
Beate
B
Wolfes
Heiner
H
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D004921:Erythropoietin / Q000494:pharmacology; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D011401:Promoter Regions, Genetic / Q000235:genetics; D000090063:Proto-Oncogene Mas; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D019151:Peptide Library; D000090063:Proto-Oncogene Mas; D020598:Proto-Oncogene Proteins c-myb; C110420:RCD1 protein, S pombe; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D004921:Erythropoietin