DRF 3188 a novel semi-synthetic analog of andrographolide: cellular response to MCF 7 breast cancer cells.
BACKGROUND
We determined the effect of andrographolide and one of its novel semi-synthetic analog, DRF 3188, on the cell cycle of MCF 7 breast cancer cells.


METHODS
The effect of the compounds on cell cycle was determined using FACS and western blot analysis of cell cycle proteins. Hollow fibre assay was used to determine if the compounds had the same effect on the cell cycle in vitro and in vivo.


RESULTS
Our results from the in vitro and in vivo experiments show that both the compounds block the cell cycle at the G0-G1 phase through the induction of the cell cycle inhibitor, p27, and the concomitant decrease in the levels of Cdk4.


CONCLUSIONS
The results show that the novel semi-synthetic analog, DRF3188, and andrographolide bring about the anti cancer activity by a similar mechanism.
Satyanarayana
Chitkala
C
Discovery Research, Dr, Reddy's Laboratories, Bollaram Road, Miyapur, Hyderabad-500049, India. chitkala@drreddys.com
Deevi
Dhanavanthri S
DS
Rajagopalan
R
R
Srinivas
Nanduri
N
Rajagopal
Sriram
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; C488658:DRF3188; D004224:Diterpenes; C030419:andrographolide