[Determination of trace antimong in cells by hydride generation-atomic fluorescence spectrometry].
An new and simple method to determine the antimony (Sb) in cells using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) was established for studying the mechanism of metal resistance in the protozoan parasite Leishmania. The detection limit was 0.08 microgram/L respectively. The average recoveries at different concentrations were 92.8%-103.6%. This method was simple, precise, reliable. The obtained results were compared with those determined by ICP-MS, and no obvious differences could be observed. This method has been successfully applied to the mechanism study of metal resistance in Leishmania.
Xie
Qing
Q
School of Public Health, Peking University, Beijing 100083, China.
Wang
Jingyu
J
Wang
Xiaoyan
X
Wang
Naifen
N
D000818:Animals; D000965:Antimony / Q000032:analysis*; D007891:Leishmania / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods*
D000965:Antimony