In vitro hydroxyapatite adsorbed salivary proteins.
In spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified.
Vitorino
Rui
R
Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Lobo
Maria João C
MJ
Duarte
José
J
Ferrer-Correia
António J
AJ
Tomer
Kenneth B
KB
Dubin
Joshua R
JR
Domingues
Pedro M
PM
Amado
Francisco M L
FM
D017886:Durapatite / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010449:Peptide Mapping; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin
D012471:Salivary Proteins and Peptides; D017886:Durapatite; D014357:Trypsin