Molecular characterisation of SENV and TTV infections in hepatopathic liver-transplant patients. Brief Report.
The presence of SENV and TTV infections among 50 patients who had undergone liver transplantation was evaluated. UTR amplification showed that 46 (92%) sera were positive. ORF-1 amplification showed that 25 (50%) patients were positive for either SENV (51.3%), TTV (10.8%), or both (37.8%) all confirmed by sequencing and phylogenetic analysis. SENV-D and SENV-H were the most prevalent viruses. The phylogenetic analysis of isolates showed that whereas SENV-D and SENV-G viruses showed sequence stability and strain persistence, SENV-H had cleared or mutated. Biological differences seem to exist among different genotypes in terms of viral replication and their persistence.
Genovese
D
D
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Dettori
S
S
Argentini
C
C
Kondili
L A
LA
La Sorsa
V
V
Tisone
G
G
Angelico
M
M
Rapicetta
M
M
D020121:5' Untranslated Regions; D018136:Circoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018173:Circoviridae Infections / Q000821:virology*; D004279:DNA, Viral / Q000097:blood / Q000737:chemistry; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D020121:5' Untranslated Regions; D004279:DNA, Viral