title: Analyzing cell cycle checkpoints after ionizing radiation.
abstract: Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.
authors.affiliation: Department of Genetics and Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Michael B
authors.initials: MB
authors.lastname: Kastan
mesh_terms: D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation / Q000528:radiation effects*; D011839:Radiation, Ionizing; D011838:Radiation-Sensitizing Agents
chemical_list: D006657:Histones; D011838:Radiation-Sensitizing Agents; D004247:DNA; D001973:Bromodeoxyuridine