Effects of ochratoxin a on oxidative damage in rat kidney: protective role of melatonin.
Epidemiological studies indicate that ochratoxin A (OTA) may be involved in the pathogenesis of different forms of human nephropathies. Melatonin (MEL) displays antioxidant and free radical scavenger properties. In the present study, the effect of MEL on the oxidative stress induced by OTA administration in rats was investigated. Three groups of eight rats each were used: control, OTA (289 micro g kg(-1) day(-1)) and OTA + MEL (OTA, 289 micro g kg(-1) day(-1); MEL, 10 mg kg(-1) day(-1)), with treatment at two different time periods during the same day. After 4 weeks of treatment, the levels of lipid peroxidation (LPO) and the activities of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) were measured in homogenates of kidney. In OTA-treated rats, the levels of LPO and the activities of GSHPx and SOD were significantly decreased compared with controls. In OTA + MEL-treated rats, SOD, GSHPx and catalase activities and LPO levels were not changed significantly in comparison with controls.
Ozçelik
Nurten
N
Department of Medical Biology and Genetics, Suleyman Demirel University, Faculty of Medicine, 32260 Isparta, Turkey. nozcelik@med.sdu.edu.tr
Soyöz
Mustafa
M
Kilinç
Ibrahim
I
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000627:therapeutic use*; D009793:Ochratoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002273:Carcinogens; D016166:Free Radical Scavengers; D009793:Ochratoxins; C025589:ochratoxin A; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008550:Melatonin