Ultrastructures of mechanoreceptors in the oral mucosa.
The present review describes the fine structures of lamellated mechanoreceptive corpuscles, Merkel cell-neurite complexes and free nerve endings in the oral mucosae of mammals, with special attention to axon terminals and lamellar cells. The mechanoreceptive nerve endings of the oral mucosa were studied using histochemistry, immunohistochemistry and transmission electron microscopy techniques. The organized mechanoreceptive corpuscles are present in the mucosae of gingiva, cheek, tongue and soft and hard palate. They are elongated or globular in shape, being located in the connective tissue papillae. The capsule is composed of several layers of cytoplasmic extensions of perineural cells. Numerous bundles of collagen fibers are noted at the periphery of the corpuscle. The lamellated corpuscles are surrounded by several layers of superimposed flattened capsular cell processes. The interlamellar spaces are 0.2-0.4 micron in width and filled with thin fibrillar collagen fibers embedded in the amorphous substance. The lamellar cells contain rich microtubules and are characterized by the presence of caveolae on the surface plasma membrane. The terminal axon contains an abundance of mitochondria and small clear vesicles (20-50 nm in diameter). There are neurofilaments in the center of the axon terminal. Intermediate-type junctions are seen between the adjacent lamellar cells and between the axon and adjacent lamellae. The free nerve endings are found in the subepithelial regions, very close to the basal laminae of mucosal epithelium. They are surrounded by a thin cytoplasm of Schwann cells. Sometimes Schwann cell basal larinae become multilayered. Merkel cells are present within the basal layer of mucosal epithelium and contain characteristic electron-dense granules that are located almost exclusively at the side of cytoplasm in contact with axon terminals. Intermediate-type junctions are noted between axon terminals and Merkel cells.
Watanabe
Ii-sei
IS
Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Avenue Prof Lineu Prestes, 2415 Cep, 05508-900, São Paulo, SP Brazil. watanabe@icb.usp.br
D000818:Animals; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D003094:Collagen / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D008465:Mechanoreceptors / Q000502:physiology / Q000648:ultrastructure*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D018862:Merkel Cells / Q000502:physiology / Q000648:ultrastructure; D009061:Mouth Mucosa / Q000294:innervation* / Q000502:physiology / Q000648:ultrastructure; D012583:Schwann Cells / Q000502:physiology / Q000648:ultrastructure; D011984:Sensory Receptor Cells / Q000502:physiology / Q000648:ultrastructure
D003094:Collagen