A comparison of commercial kit methods for assay of vitamin b(12) in ruminant blood.
Five commercially available radioisotope dilution (RID) kits for assay of Vitamin B(12) were obtained for evaluation with ovine and bovine serum; also, for comparison with our laboratory's (Lab 1) RID method and the methods of two independent laboratories, one using RID and the other a microbiological method. One commercial kit was found to be suitable for both species because negligible non-specific binding occurred when extraction was performed at 100 degrees C (212 degrees F). Of the other four kits, two were found to be suitable for ovine and bovine serum provided non-specific binding of the latter was measured. This was also the case with the Lab 1 method. Two of the commercial kits were found to be unsuitable for assay of Vitamin B(12)in ruminant blood.
Schultz
W J
WJ
Central Veterinary Laboratories, Department of Agriculture, I.M.V.S., Frome Road, Adelaide, South Australia 5000.