title: Impaired glucose-stimulated insulin secretion, enhanced intraperitoneal insulin tolerance, and increased beta-cell mass in mice lacking the p110gamma isoform of phosphoinositide 3-kinase.
abstract: Phosphoinositide 3-kinase (PI3 kinase) has been implicated in G protein-coupled receptor regulation of pancreatic beta-cell growth and glucose-stimulated insulin secretion. The G protein-activated p110gamma isoform of PI3 kinase was detected in insulinoma cells, mouse islets, and human islets. In 7- to 10-wk-old mice, knockout of p110gamma reduced the plasma insulin response to ip glucose injection and impaired first and second phase glucose-stimulated insulin secretion from pancreata perfused ex vivo. The p110gamma -/- mice responded to preinjection with the glucagon-like peptide-1 receptor agonist exendin 4, such that plasma glucose and insulin responses to ip glucose injection were not different from wild types. Mice lacking p110gamma were not diabetic and were only slightly glucose intolerant (ip glucose injection) compared with wild types, in part due to enhanced responsiveness to insulin as determined by an ip insulin tolerance test. Despite severely reduced insulin secretion in these animals, the p110gamma -/- mice had greater pancreatic insulin content, and an increased beta-cell mass due to beta-cell hypertrophy. These surprising results suggest that the G protein-coupled p110gamma isoform of PI3 kinase is not central to the development or maintenance of sufficient beta-cell mass but positively regulates glucose-stimulated insulin secretion.
authors.affiliation: University of Toronto, Department of Physiology, Toronto, Canada. patrick.macdonald@mphy.lu.se.
authors.forename: P E
authors.initials: PE
authors.lastname: MacDonald
authors.forename: J W
authors.initials: JW
authors.lastname: Joseph
authors.forename: D
authors.initials: D
authors.lastname: Yau
authors.forename: J
authors.initials: J
authors.lastname: Diao
authors.forename: Z
authors.initials: Z
authors.lastname: Asghar
authors.forename: F
authors.initials: F
authors.lastname: Dai
authors.forename: G Y
authors.initials: GY
authors.lastname: Oudit
authors.forename: M M
authors.initials: MM
authors.lastname: Patel
authors.forename: P H
authors.initials: PH
authors.lastname: Backx
authors.forename: M B
authors.initials: MB
authors.lastname: Wheeler
mesh_terms: D000818:Animals; D058544:Class Ib Phosphatidylinositol 3-Kinase; D000077270:Exenatide; D005947:Glucose / Q000494:pharmacology; D006706:Homeostasis / Q000502:physiology; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000201:enzymology* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010455:Peptides / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D014688:Venoms / Q000494:pharmacology
chemical_list: D007328:Insulin; D007527:Isoenzymes; D010455:Peptides; D014688:Venoms; D000077270:Exenatide; D019869:Phosphatidylinositol 3-Kinases; D058544:Class Ib Phosphatidylinositol 3-Kinase; C546760:PIK3CG protein, human; C546764:Pik3cg protein, mouse; D005947:Glucose