In vitro investigation on the impact of Solutol HS 15 on the uptake of colchicine into rat hepatocytes.
In the current investigation, the impact of the surface-active formulation ingredient Solutol HS 15 on the uptake of colchicine into freshly isolated rat hepatocytes was investigated using a centrifugal filtration technique through a silicone oil layer. Colchicine is taken up into the cells by an active transport mechanism. When conducting the experiment at 37 degrees C, it was found that at concentrations below its critical micellar concentration (CMC) of 0.021% (0.0003 and 0.003%, w/v), Solutol HS 15 did not impact the uptake of colchicine. By contrast, at a Solutol HS 15 concentration above its CMC (0.03%, w/v), the amount of colchicine taken up into the cells as well as its uptake velocity were significantly decreased. However, in control experiments performed at 4 degrees C, a temperature at which active transport processes should be significantly slowed down, Solutol HS 15 at 0.03% did not affect colchicine uptake and/or its association with the cells. The described findings might be rationalized by inhibition of colchicine transport either due to direct interaction at the transport site or due to alterations of membrane properties in the presence of Solutol HS 15 at concentrations above its CMC. Moreover, a strong molecular interaction between Solutol HS 15 and colchicine as well as an incorporation of colchicine into micelles formed by Solutol HS 15, this way resulting in a limited contact of colchicine with the cells, cannot be excluded as contributors to the observed effect.
Bravo González
Roberto Carlos
RC
Pharma Research, Discovery DMPK, F. Hoffmann-La Roche Ltd., Grenzacher Strasse 124, CH-4070 Basel, Switzerland.
Boess
Franziska
F
Durr
Evelyne
E
Schaub
Nathalie
N
Bittner
Beate
B
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003078:Colchicine / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013229:Stearic Acids / Q000494:pharmacology*; D013696:Temperature
D013229:Stearic Acids; D011092:Polyethylene Glycols; C067028:Solutol HS 15; D003078:Colchicine