Arabidopsis RADICAL-INDUCED CELL DEATH1 belongs to the WWE protein-protein interaction domain protein family and modulates abscisic acid, ethylene, and methyl jasmonate responses.
Experiments with several Arabidopsis thaliana mutants have revealed a web of interactions between hormonal signaling. Here, we show that the Arabidopsis mutant radical-induced cell death1 (rcd1), although hypersensitive to apoplastic superoxide and ozone, is more resistant to chloroplastic superoxide formation, exhibits reduced sensitivity to abscisic acid, ethylene, and methyl jasmonate, and has altered expression of several hormonally regulated genes. Furthermore, rcd1 has higher stomatal conductance than the wild type. The rcd1-1 mutation was mapped to the gene At1g32230 where it disrupts an intron splice site resulting in a truncated protein. RCD1 belongs to the (ADP-ribosyl)transferase domain-containing subfamily of the WWE protein-protein interaction domain protein family. The results suggest that RCD1 could act as an integrative node in hormonal signaling and in the regulation of several stress-responsive genes.
Ahlfors
Reetta
R
Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, 00014, Finland.
Lång
Saara
S
Overmyer
Kirk
K
Jaspers
Pinja
P
Brosché
Mikael
M
Tauriainen
Airi
A
Kollist
Hannes
H
Tuominen
Hannele
H
Belles-Boix
Enric
E
Piippo
Mirva
M
Inzé
Dirk
D
Palva
E Tapio
ET
Kangasjärvi
Jaakko
J
D000040:Abscisic Acid / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D054883:Oxylipins; D020161:Physical Chromosome Mapping; D030821:Plants, Genetically Modified
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D009687:Nuclear Proteins; D054883:Oxylipins; C492758:RCD1 protein, Arabidopsis; D000040:Abscisic Acid; C072239:methyl jasmonate; C036216:ethylene; D005947:Glucose