title: TROD: T7 RNAi Oligo Designer.
abstract: We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.
authors.affiliation: Département de Biologie Cellulaire, Université de Genève, Sciences III, Quai Ernest-Ansermet 30, CH-1211 Genève 4, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Dudek
authors.forename: Didier
authors.initials: D
authors.lastname: Picard
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D020407:Internet; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000096:biosynthesis* / Q000737:chemistry; D012984:Software; D014764:Viral Proteins
chemical_list: D009838:Oligodeoxyribonucleotides; D034741:RNA, Small Interfering; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases