title: Preparation of porcine carotid arteries for vascular tissue engineering applications.
abstract: Biomaterials derived from tissue continue to offer viable alternatives to synthetic materials when autologous materials are unavailable for transplantation due to their unique chemical and mechanical properties. Tissue processing aims to stabilize the material against host degradation and render it immunologically inert by removing cellular material and crosslinking the structural proteins. It is clear that different approaches taken to achieve these goals have very different chemical and mechanical effects on the material. We describe herein the development of a tissue processing methodology to generate acellular scaffolds for tissue engineering small-diameter vascular grafts. Carotid arteries were isolated from Great White pigs and exposed to various solvent treatments, xylene, butanol, and ethanol to determine optimal parameters for the extraction of host lipids. The tissue was then exposed to a limited proteolysis with trypsin to disrupt cellular protein. This resulted in a controlled digestion that disrupted porcine nuclear DNA and cleared bulk cellular protein, leaving the more resistant structural proteins largely intact and retaining the bulk mechanical properties of the matrix. Histological analysis and scanning electron microscopy illustrated the complete removal of intact cells and nuclear material. The decellularized graft was stabilized by crosslinking with the photooxidative dye methylene green in the presence of 30,000 LUX of broad-band light energy. High-performance liquid chromatography analysis showed that the crosslinked tissue yielded 78.6% less hydroxyproline, compared with control tissue, after 20 h incubation with pepsin. Analysis of the crosslinked vessels' burst-pressure and stress-strain characteristics have shown comparable mechanical properties to those of control vessels. Assessment of in vitro cell adhesion and compatibility was conducted by seeding primary human umbilical vein endothelial cells and adult human vascular smooth muscle cells onto the lumenal and ablumenal surfaces, respectively; these cells were shown to adhere and proliferate under traditional static culture conditions.
authors.affiliation: School of Chemical Engineering and Materials Science, University of Oklahoma, 100 East Boyd, Norman, Oklahoma 73019-1004, USA.
authors.forename: Peter S
authors.initials: PS
authors.lastname: McFetridge
authors.forename: Joel W
authors.initials: JW
authors.lastname: Daniel
authors.forename: Tulin
authors.initials: T
authors.lastname: Bodamyali
authors.forename: Michael
authors.initials: M
authors.lastname: Horrocks
authors.forename: Julian B
authors.initials: JB
authors.lastname: Chaudhuri
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D012997:Solvents; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D014280:Triglycerides / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D012997:Solvents; D014280:Triglycerides; D014357:Trypsin