title: Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip.
abstract: A simple method was developed for injecting a sample on a cross-form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gai
authors.forename: Linfen
authors.initials: L
authors.lastname: Yu
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Dai
authors.forename: Yinfa
authors.initials: Y
authors.lastname: Ma
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D000818:Animals; D002347:Carps / Q000097:blood*; D004574:Electromagnetic Fields; D046209:Electrophoresis, Microchip; D004912:Erythrocytes / Q000166:cytology*; D005453:Fluorescence; D006874:Hydrostatic Pressure; D044085:Microfluidics