Virus PCR assay panels: an alternative to the mouse antibody production test.
Antibody production tests have traditionally been used to test biological materials for viral contamination. Now molecular biology techniques have emerged as an alternative. The authors compare MAP testing with PCR-based detection methods, focusing on differences in animal use, laboratory requirements, sample size, and limits of detection.
Blank
Walter A
WA
Charles River Laboratories, 251 Ballardvale Street, Wilmington. MA 01887, USA.
Henderson
Kenneth S
KS
White
Lisa A
LA
D000818:Animals; D000830:Animals, Laboratory / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D007159:Immunologic Tests / Q000379:methods*; D051379:Mice; D016133:Polymerase Chain Reaction; D012376:Rodent Diseases / Q000175:diagnosis / Q000821:virology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary*
D000914:Antibodies, Viral