title: Genus and species-specific IgG and IgM antibodies for pulmonary tuberculosis.
abstract: OBJECTIVE
  To evaluate three different enzyme immunoassays for serological diagnosis of pulmonary tuberculosis and to compare their diagnostic accuracy in different combinations.
  
  
  METHODS
  A non-interventional comparative study.
  
  
  METHODS
  The study was carried out at the Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi between April and September 2001.
  
  
  METHODS
  Sera from patients suffering from pulmonary tuberculosis (n=94) with sputum positive for acid fast bacilli (AFB) and sera from control group of healthy individuals (n=90) with sputum negative for AFB were tested by Pathozyme-Myco G EIA, Pathozyme-TB Complex Plus EIA and Pathozyme Myco M EIA kits for the genus-specific IgG and IgM, and the species-specific IgG antibodies against antigens of Mycobacterium tuberculosis.
  
  
  RESULTS
  The detection of IgG against genus-specific antigens by Pathozyme-Myco G had a sensitivity of 46% and a specificity of 93%, of IgG against species-specific antigens by Pathozyme-TB Complex Plus had a sensitivity of 64% and specificity of 97% and of IgM against genus-specific antigens by Pathozyme Myco M had a sensitivity of 67% and specificity of 98%. When the results of these immunoassays were evaluated in combination, their sensitivity improved. Combination of genus-specific IgM and species-specific IgG yielded best results with a sensitivity of 87% and specificity of 93%.
  
  
  CONCLUSIONS
  The sensitivity of serological diagnosis of tuberculosis is low, but it can be increased by utilizing a combination of several antigens.
authors.affiliation: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi. tariqbutt24@yahoo.com
authors.forename: Tariq
authors.initials: T
authors.lastname: Butt
authors.forename: Hamid Saeed
authors.initials: HS
authors.lastname: Malik
authors.forename: Shahid Ahmad
authors.initials: SA
authors.lastname: Abbassi
authors.forename: Rifat Nadeem
authors.initials: RN
authors.lastname: Ahmad
authors.forename: Abid
authors.initials: A
authors.lastname: Mahmood
authors.forename: Karamat A
authors.initials: KA
authors.lastname: Karamat
authors.forename: Masood
authors.initials: M
authors.lastname: Anwar
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic