title: Monocytes of distinct clinical types of leprosy are differentially activated by cross-linking class II HLA molecules to secrete IL-12.
abstract: Leprosy is characterized by a wide spectrum of clinical features depending on the individual differences in Th1-type immunity. The objective of this study was to evaluate whether monocyte activation by stimulus via class II HLA molecules would be correlated with the differences in cellular immune responses among diverse clinical forms of leprosy. IL-1beta and IL-12 productivity in monocyte preparations obtained from PBMCs was estimated in patients with lepromatous- and tuberculoid-type leprosy. We found that monocytes from lepromatous patients produced significantly higher (about 4-fold higher) amounts of IL-12 as compared to in patients with tuberculoid type of leprosy when class II HLA molecules were cross-linked with anti-HLA class II antibodies, whereas almost equal amounts of IL-1beta were produced from each monocyte preparation by stimulus via class II HLA molecules regardless of the clinical form of leprosy. These results suggest that monocyte activation differs between lepromatous and tuberculoid patients in terms of IL-12 secretion, which might be related to individual differences in the cellular immune responses according to the clinical type of leprosy.
authors.affiliation: Department of Allergy and Immunology, Saitama Medical School, Morohongo, Moroyama, Japan. imohyama@saitama-med.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohyama
authors.forename: Nahoko
authors.initials: N
authors.lastname: Kato
authors.forename: Kazu
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Soga
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uemura
authors.forename: Fusanori
authors.initials: F
authors.lastname: Nishimura
authors.forename: Sho
authors.initials: S
authors.lastname: Matsushita
mesh_terms: D016207:Cytokines / Q000097:blood / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D018664:Interleukin-12 / Q000097:blood / Q000378:metabolism*; D007918:Leprosy / Q000097:blood / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D012016:Reference Values
chemical_list: D016207:Cytokines; D006681:HLA-D Antigens; D018664:Interleukin-12