[Screening of antagonist peptide of BLyS from C7C phage display peptide library].
OBJECTIVE
To screen an antagonist peptide of BLyS from C7C phage display peptide library.


METHODS
C7C phage display peptide library was screened with BLyS. Indirect ELISA, competitive ELISA and MTT colorimetry were used to identify positive phage clones.


RESULTS
After 3 rounds of screening, the gradual increase of the ratio of output to input and specific enrichment had been achieved. Two phage clones that could inhibit the BLyS activity were identified.


CONCLUSIONS
Two phage display antagonist peptides of BLyS were obtained.
Ji
Qing
Q
Department of Biochemistry & Molecular Biology, Third Military Medical University, Chongqing 400038, China.
He
Feng-tian
FT
Li
Rong-fen
RF
Gao
Hui-guang
HG
Zheng
Ying-ru
YR
Zhong
Xiao-lin
XL
Zheng
Han-qi
HQ
D053264:B-Cell Activating Factor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D053264:B-Cell Activating Factor; D008565:Membrane Proteins; D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; C505719:TNFSF13B protein, human; D014409:Tumor Necrosis Factor-alpha