Detection of regional changes in protein levels in the in vivo canine model of acute heart failure following ischemia-reperfusion injury: functional proteomics studies.
In this study, we demonstrate the use of proteomics to detect regional differences in protein levels between the reperfused ischemic zone (IZ) and the nonischemic zone (NIZ) of dog hearts which were subjected to in vivo ischemia-reperfusion injury. Using the two-dimensional gel electrophoresis (2-DE) technique, we identified five proteins that were differentially expressed in the IZ versus NIZ: (1) the alpha subunit of ATP synthase isoform precursor was decreased 1.71-fold; (2) creatine kinase M chain was decreased 1.72-fold; (3) NAD+-isocitrate dehydrogenase, alpha subunit was increased 8.34-fold; (4) ATP synthase D chain, mitochondrial was increased 3.02-fold; (5) ventricular myosin light chain-1 was decreased 2.02-fold. Additionally, we found that the level of actin was decreased 2.6-times in the IZ compared to the NIZ on Western blot analysis but was unchanged on 2-DE.
Sawicki
Grzegorz
G
Department of Pharmacology, and Cardiovascular Research Group, Faculty of Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada. Greg.Sawicki@UAlberta.CA
Jugdutt
Bodh I
BI
D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D019992:Databases as Topic; D003720:Densitometry; D004285:Dogs; D004452:Echocardiography; D015180:Electrophoresis, Gel, Two-Dimensional; D006333:Heart Failure / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007511:Ischemia; D013058:Mass Spectrometry; D008953:Models, Anatomic; D020033:Protein Isoforms; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D006180:Proton-Translocating ATPases / Q000737:chemistry; D015427:Reperfusion Injury; D012984:Software; D013997:Time Factors
D000199:Actins; D020033:Protein Isoforms; D020543:Proteome; D006180:Proton-Translocating ATPases