Human immunodeficiency virus 1 downregulates cell surface expression of the non-classical major histocompatibility class I molecule HLA-G1.
Human immunodeficiency virus 1 (HIV-1) downregulates cell surface expression of HLA-A and HLA-B but not HLA-C or HLA-E to ultimately escape immune defences. Here, it is shown that cell surface expression of the non-classical HLA-G1 is also downregulated by HIV-1, by using co-transfection experiments and infection with cell-free HIV-1 of HLA-G1-expressing U87 glioma cells or macrophages in primary culture. Moreover, co-transfection experiments using proviruses deleted in either nef or vpu or plasmids encoding HIV-1 Nef and Vpu mixed together with a HLA-G1-expressing construct demonstrated that HLA-G1 downregulation is Nef-independent and Vpu-dependent, contrasting with the Nef- and Vpu-dependent HLA-A2 downregulation. Together, these results show that the decrease of HLA-A2 and HLA-G1 caused by HIV-1 occurs through distinct mechanisms.
Derrien
Muriel
M
Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Pizzato
Nathalie
N
INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
Dolcini
Guillermina
G
Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Menu
Elisabeth
E
INSERM U131, 32 rue des Carnets, 92140 Clamart, France.|Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Chaouat
Gérard
G
INSERM U131, 32 rue des Carnets, 92140 Clamart, France.
Lenfant
Françoise
F
INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
Barré-Sinoussi
Françoise
F
Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Bouteiller
Philippe Le
PL
INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
D000918:Antibody Specificity; D002460:Cell Line; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology
D006680:HLA Antigens; D015789:HLA-A2 Antigen; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D007132:Immunoglobulin Isotypes