title: The X protein of Borna disease virus regulates viral polymerase activity through interaction with the P protein.
abstract: Borna disease virus polymerase activity is negatively regulated by the viral X protein. Using a virus minireplicon system it was found that all X mutants that no longer interacted with the viral P protein failed to exhibit significant inhibitory activity. The action of X could further be neutralized by expression of a P fragment that contained the X interaction domain but lacked all domains known to mediate interaction with other viral proteins. X thus appears to regulate the activity of the Borna disease virus polymerase by targeting the polymerase cofactor P.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Poenisch
authors.affiliation: Robert-Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Gunhild
authors.initials: G
authors.lastname: Unterstab
authors.affiliation: Robert-Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wolff
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
authors.forename: Urs
authors.initials: U
authors.lastname: Schneider
mesh_terms: D001891:Borna disease virus / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D016679:Genome, Viral; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases