Accumulation of spermidine/spermine N1-acetyltransferase and alternatively spliced mRNAs as a delayed response of HeLa S3 cells following X-ray irradiation.
OBJECTIVE
A key enzyme of polyamine catabolism, spermidine/spermine N(1)-acetyltransferase (SSAT), is responsive to antiproliferative agents. The role of SSAT in cellular responses to X-ray irradiation was examined.


METHODS
Exponentially growing HeLa S3 cells were irradiated by X-rays, and mRNA levels for SSAT were measured as a function of post-irradiation time through Northern hybridization. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect alternatively spliced SSAT mRNAs. The intracellular polyamine content was measured by the o-phthalaldehyde method and the enzymatic activity of SSAT by the increased amount of acetylated spermidine after incubation.


RESULTS
Not only SSAT mRNA, but also an alternatively spliced mRNA accumulated at the initial stage of growth inhibition after the first or second replication of irradiated cells. The maximum fold increase relative to the level of non-irradiated cells was 3.0-3.5 for both transcripts after 5-Gy irradiation. On the other hand, the mRNA of ornithine decarboxylase, a key enzyme of polyamine synthesis, was little influenced by X-ray treatment. Enzymatic activity of SSAT and the acetylspermidine level were elevated after X-ray irradiation.


CONCLUSIONS
Activation of SSAT and the induction of alternatively spliced mRNA of the SSAT gene play an important role in regulating growth inhibition and cell death after X-ray irradiation.
Mita
K
K
Laboratory of Insect Genome, Department of Genome Research National Institute of Agrobiological Sciences Owashi 1-2, Tsukuba, Ibaraki 305-8634 Japan. ichimura@nirs.go.jp
Fukuchi
K
K
Hamana
K
K
Ichimura
S
S
Nenoi
M
M
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D000222:Adaptation, Physiological / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation / Q000235:genetics / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D022821:RNA Splice Sites / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D011829:Radiation Dosage; D014965:X-Rays; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D022821:RNA Splice Sites; D012333:RNA, Messenger; D000099228:Diamine N-Acetyltransferase