Analyzing cell cycle checkpoints after ionizing radiation.
Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.
Xu
Bo
B
Department of Genetics and Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, USA.
Kastan
Michael B
MB
D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation / Q000528:radiation effects*; D011839:Radiation, Ionizing; D011838:Radiation-Sensitizing Agents
D006657:Histones; D011838:Radiation-Sensitizing Agents; D004247:DNA; D001973:Bromodeoxyuridine