[A study on the relationship between interleukin-10 promoter polymorphism and autoimmune liver disease].
OBJECTIVE
To investigate whether three biallelic polymorphisms at the position -592, -819 and -1082 in the promoter region of the IL-10 gene were associated with the incidence of autoimmune liver disease.


METHODS
The IL-10 -592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphisms analysis (PCR-RFLP), while polymerase chain reaction- sequence specific primer (PCR-SSP) assay was used to detect IL-10 -819 polymorphisms.


RESULTS
Among 54 Chinese patients with AIH or 77 Chinese patients with PBC versus healthy controls, the frequency of AA, GA genotypes at IL-10 gene promoter -1082 position was 87.0% or 83.1% versus 90.0%, 13.0% or 16.9% versus 10.0%, respectively (P > 0.05), the GG genotype in Chinese populations is absent; the frequency of CC, CT, TT genotypes at IL-10 gene promoter -819 position was 11.11% or 9.1% versus 8.1%, 44.4% or 53.3% versus 45.0%, 44.4% or 37.7% versus 46.9%, respectively (P > 0.05); the frequency of CC, CA, AA genotypes at IL-10 gene promoter -592 position was 4.9% or 14.3% versus 10.0%, 51.2% or 53.3% versus 51.9%, 43.9% or 32.5% versus 38.1%, respectively (P > 0.05). No alleles differed significantly in each groups.


CONCLUSIONS
There were no association between IL-10 gene polymorphisms and autoimmune liver disease
Chen
Qu-bo
QB
The Second Institute of Clinical Medicine, Guangzhou University of TCM, Guangzhou 510120, China.
Fan
Lie-ying
LY
Zhong
Ren-qian
RQ
Tu
Xiao-qing
XQ
Yuan
Yuan
Y
Zhu
Ye
Y
Ye
Wei-min
WM
Lu
Hui-qi
HQ
Han
Hui-xing
HX
D000328:Adult; D000368:Aged; D005260:Female; D019693:Hepatitis, Autoimmune / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008105:Liver Cirrhosis, Biliary / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics
D016753:Interleukin-10