title: Mechanism of action of the suppression of herpes simplex virus type 2 replication by pterocarnin A.
abstract: The purpose of this study was to investigate the in vitro antiviral properties of pterocarnin A, extracted from the bark of Pterocarya stenoptera (Juylandaceae). Results showed that pterocarnin A exhibited anti-herpes simplex virus (HSV) activity. It had a low selectivity index (SI) value and only possessed some level of cell cytotoxic effect at high antiviral concentrations. Mechanism studies demonstrated that pterocarnin A inhibited herpes simplex virus type 2 (HSV-2) from attaching and penetrating into cells. It also actively suppressed HSV-2 multiplication in Vero cells even when added 12 h after infection. This observation indicated that pterocarnin A affected the late stage(s) of HSV-2 infection cycle. Pterocarnin A also significantly reduced viral infectivity at high concentrations. From these observations, it was concluded that pterocarnin A suppressed both early and late in the replication cycle of HSV-2. The various modes of action of pterocarnin A in interfering with certain steps of viral infection thus merit further investigation.
authors.affiliation: Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, 807 Kaohsiung, Taiwan, ROC.
authors.forename: Hua-Yew
authors.initials: HY
authors.lastname: Cheng
authors.forename: Ta-Chen
authors.initials: TC
authors.lastname: Lin
authors.forename: Chien-Min
authors.initials: CM
authors.lastname: Yang
authors.forename: Kuo-Chih
authors.initials: KC
authors.lastname: Wang
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002522:Chlorocebus aethiops; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000633:toxicity; D006027:Glycosides / Q000494:pharmacology* / Q000633:toxicity; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000502:physiology*; D029525:Juglandaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D014709:Vero Cells; D038301:Virus Inactivation; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D006027:Glycosides; D010936:Plant Extracts; C496413:pterocarnin A; D005707:Gallic Acid