Homology between two different Salmonella phages: Salmonella enterica serovar Typhimurium phage P22 and Salmonella enterica serovar Anatum var. 15 + phageepsilon34.
A distinguishing feature of many microorganisms, belonging to the Gram negative group of bacteria, is the presence of the lipopolysaccharide on their cell surface. Salmonella is a prominent member of this group of bacteria. Many Salmonella phages use the LPS as the initial receptor in the infection process and they can distinguish subtle changes in the LPS molecules. The phage protein that is responsible for recognition of these cells is the tail or tailspike protein (TSP). Those TSPs, which use LPS as a receptor, are prokaryotic LPS-binding proteins. As an initial step in using phage TSPs as model systems for a detailed molecular genetic analysis of protein-LPS interactions, a comparison of two phages and their TSPs from two different Salmonella bacterial viruses (phages), Salmonella enterica serovar Typhimurium phage P22 and Salmonella enterica serovar Anatum var. 15 + phage epsilon34, is being carried out. This present study shows significant viral protein homology between many viral structural proteins from these two phages including their TSPs. Significantly this report suggests a general structural motif for part of the TSP of phages and suggests that a more detailed comparative analysis of these TSPs is warranted.
Salgado
Clari J
CJ
UCC School of Medicine, Bayamon, Puerto Rico 00960.
Zayas
Milka
M
Villafane
Robert
R
D000818:Animals; D017100:Bacteriophage P22 / Q000235:genetics* / Q000378:metabolism; D001434:Bacteriophage Typing; D019143:Evolution, Molecular; D006026:Glycoside Hydrolases; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D012481:Salmonella Infections, Animal / Q000821:virology; D012483:Salmonella Phages / Q000235:genetics* / Q000378:metabolism; D019779:Salmonella enterica / Q000821:virology*; D012486:Salmonella typhimurium / Q000821:virology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D017376:Viral Tail Proteins / Q000235:genetics / Q000378:metabolism
D008070:Lipopolysaccharides; D015678:Viral Structural Proteins; D017376:Viral Tail Proteins; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage