Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent.
We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and 83 microM of phyA.
Yang
Changhuei
C
Department of Biomedical Engineering, 136 Hudson Hall, Duke University, Durham, North Carolina 27708, USA. chyang@caltech.edu
Choma
Michael A
MA
Lamb
Laura E
LE
Simon
John D
JD
Izatt
Joseph A
JA
D003287:Contrast Media / Q000032:analysis / Q000737:chemistry*; D005217:Fat Emulsions, Intravenous / Q000032:analysis* / Q000737:chemistry; D005240:Feasibility Studies; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry; D010834:Phytochrome / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D051942:Phytochrome A; D041623:Tomography, Optical Coherence / Q000379:methods*
D003287:Contrast Media; D005217:Fat Emulsions, Intravenous; D015335:Molecular Probes; D051942:Phytochrome A; D010834:Phytochrome