Wnt-1 expression in PC12 cells induces exon 15 deletion and expression of L-APP.
The metabolism of amyloid precursor protein (APP) is central to Alzheimer's disease pathogenesis. Recent data have linked APP and presenilin to the Wnt/wingless signaling pathway. To assess affects of Wnt stimulation on APP isoform expression, we infected PC12 cells and C57MG cells with a retrovirus containing murine Wnt-1. In PC12 cells, Wnt-1 expression is associated with induction of exon 15 deletion from APP mRNA and expression of L-APP. Our data suggest that APP isoform expression is regulated, in part, by the Wnt/wingless signaling pathway.
Morin
Peter J
PJ
Center for Neurologic Diseases, Brigham and Women's Hospital, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA. morin@bu.edu
Medina
Miguel
M
Semenov
Mikhail
M
Brown
Anthony M C
AM
Kosik
Kenneth S
KS
D017398:Alternative Splicing / Q000235:genetics; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D005091:Exons / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D016716:PC12 Cells; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D017384:Sequence Deletion; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D051153:Wnt Proteins; D051155:Wnt1 Protein
D016564:Amyloid beta-Protein Precursor; D020033:Protein Isoforms; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D051155:Wnt1 Protein; C495246:Wnt1 protein, mouse; C495245:Wnt1 protein, rat