title: A biomarker approach to endocrine disruption in flounder--estrogen receptors, hepatocyte proliferation, and sperm motility.
abstract: Three different physiological parameters were assessed to determine their potential for serving as biomarkers to predict abnormally elevated vitellogenin (VTG) production in male and immature flounder. Whereas abnormally elevated mean VTG plasma concentrations clearly distinguished the Mersey and Dee flounder studied, the results showed no significant differences in estrogen receptor binding capacity or binding affinity between the two groups. Hepatocyte proliferation was not found to be a "biomarker of effect" that could specifically be used to assess an increase in VTG-related proliferation. Nevertheless, immunohistochemical staining for proliferating cell nuclear antigen did show a significantly higher proliferative activity in the livers of Mersey flounder than that in Dee flounder. Sperm motility also was not found to be a biomarker of effect linked to an abnormal elevation of VTG plasma concentration. The results (higher sperm quality in terms of motility in Mersey flounder) were unexpected but interesting.
authors.affiliation: School of Biological Sciences, University of Liverpool, Jones Building, Liverpool L69 3GS, UK. smileede@yahoo.com
authors.forename: Anne
authors.initials: A
authors.lastname: Kleinkauf
authors.forename: Chris
authors.initials: C
authors.lastname: Macfarlane
authors.forename: Sarah
authors.initials: S
authors.lastname: Yeates
authors.forename: Michael G
authors.initials: MG
authors.lastname: Simpson
authors.forename: Richard T
authors.initials: RT
authors.lastname: Leah
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002455:Cell Division / Q000187:drug effects; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004967:Estrogens / Q000633:toxicity*; D005260:Female; D005432:Flounder / Q000097:blood / Q000378:metabolism*; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011960:Receptors, Estrogen / Q000032:analysis / Q000378:metabolism*; D012623:Seawater; D013081:Sperm Motility / Q000187:drug effects; D013997:Time Factors; D006113:United Kingdom; D014819:Vitellogenins / Q000097:blood / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity
chemical_list: D015415:Biomarkers; D004967:Estrogens; D011960:Receptors, Estrogen; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D015262:Xenobiotics