Isolation of Adda from microcystin-LR by microbial degradation.
The intact Adda was isolated from microcystin-LR by a microbial degradation using an isolated Sphingomonas strain, B-9. The reaction of microcystin-LR with cell extract of this strain proceeded smoothly to give the final degradation product by way of two intermediates, linearized microcystin-LR and a tetrapeptide. The purified Adda that was structurally characterized using various spectral data did not show the toxicity to mice or inhibition to protein phosphatase activity in contrast to the native toxin.
Harada
Ken-ichi
K
Graduate School of Environmental and Human Sciences, Meijo University, Tempaku, Nagoya 468-8503, Japan. kiharada@ccmfs.meijo-u.ac.jp
Imanishi
Susumu
S
Kato
Hajime
H
Mizuno
Masayoshi
M
Ito
Emiko
E
Tsuji
Kiyomi
K
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000378:metabolism*; D001673:Biodegradation, Environmental; D000458:Cyanobacteria / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008387:Marine Toxins / Q000378:metabolism*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism
D000596:Amino Acids; D001427:Bacterial Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR