title: Chitosan sponges as tissue engineering scaffolds for bone formation.
abstract: Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.
authors.affiliation: Department of Periodontology, College of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul 110-749, South Korea.
authors.forename: Yang-Jo
authors.initials: YJ
authors.lastname: Seol
authors.forename: Jue-Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Yoon-Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Yong-Moo
authors.initials: YM
authors.lastname: Lee
authors.lastname: Young-Ku
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Rhyu
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Soo-Boo
authors.initials: SB
authors.lastname: Han
authors.forename: Chong-Pyoung
authors.initials: CP
authors.lastname: Chung
mesh_terms: D000818:Animals; D016723:Bone Remodeling; D002478:Cells, Cultured; D048271:Chitosan; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D012886:Skull / Q000166:cytology; D023822:Tissue Engineering
chemical_list: D048271:Chitosan