title: Flow cytometric screening of aldolase catalytic antibodies.
abstract: High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
authors.affiliation: The Skaggs Institute for Chemical Biology and Departments of Molecular Biology and Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Hyunbo
authors.initials: H
authors.lastname: Shim
authors.forename: Amelie
authors.initials: A
authors.lastname: Karlström
authors.forename: Sofia M
authors.initials: SM
authors.lastname: Touami
authors.forename: Roberta P
authors.initials: RP
authors.lastname: Fuller
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Barbas
mesh_terms: D000818:Animals; D017151:Antibodies, Catalytic / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D005634:Fructose-Bisphosphate Aldolase / Q000187:drug effects / Q000378:metabolism*; D006825:Hybridomas; D007700:Kinetics; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D014162:Transfection
chemical_list: D017151:Antibodies, Catalytic; D011994:Recombinant Proteins; D005634:Fructose-Bisphosphate Aldolase