title: GABA uptake via GABA transporter-1 modulates GABAergic transmission in the immature hippocampus.
abstract: GABA uptake limits GABA actions during synaptic responses when the density of active release sites is high or multiple axons are synchronously activated. GABA transporter-1 (GAT-1) is the main neuronal GABA transporter subtype and is already expressed in the early postnatal rat hippocampus. However, previous studies have demonstrated little functional role for the transporter during this developmental period. We used whole-cell voltage-clamp and field-potential recordings in hippocampal slices of neonatal rats (postnatal day 4-5) to study whether GAT-1 plays a role in GABAergic transmission during spontaneous population oscillations, which are seen as "giant depolarizing potentials" (GDPs) in intracellular recordings. We show that the GDP-associated GABAergic current observed in CA3 pyramidal neurons is strongly enhanced by the GAT-1-specific blocker NO-711 (1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride). Our results indicate a novel role for GAT-1 in the control of endogenous activity of the immature hippocampus.
authors.affiliation: Department of Biological and Environmental Sciences, University of Helsinki, FIN-00014 Helsinki, Finland.
authors.forename: Sampsa
authors.initials: S
authors.lastname: Sipilä
authors.forename: Kristiina
authors.initials: K
authors.lastname: Huttu
authors.forename: Juha
authors.initials: J
authors.lastname: Voipio
authors.forename: Kai
authors.initials: K
authors.lastname: Kaila
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000031:analogs & derivatives* / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D050485:GABA Plasma Membrane Transport Proteins; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D007395:Interneurons / Q000187:drug effects / Q000502:physiology; D026901:Membrane Transport Proteins / Q000502:physiology*; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009557:Nipecotic Acids / Q000494:pharmacology; D010091:Oximes / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects; D013779:Tetrodotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D018756:GABA Antagonists; D050485:GABA Plasma Membrane Transport Proteins; D026901:Membrane Transport Proteins; D014179:Neurotransmitter Uptake Inhibitors; D009557:Nipecotic Acids; D010091:Oximes; D011810:Quinoxalines; C493415:Slc6a1 protein, rat; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; C078793:NNC 711; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; C036615:bicuculline methobromide; D001640:Bicuculline