[Combination immunization with EIAV Env protein expressed by recombinant baculovirus and recombinant vaccinia virus containing env gene].
OBJECTIVE
To develop a novel vaccine candidate of Equine infectious anemia virus(EIAV).


METHODS
env genes of EIAV Chinese donkey leukocyte attenuated strain (EIAV DLV) and its parental virus strain (EIAV LN) were expressed using the BAC-To-BAC system, and Env proteins were confirmed by SDS-PAGE and Western blot. BALB/c mice were immunized with recombinant vaccinia viruses containing env genes of EIAV alone or boosted with Env proteins expressed by recombinant baculovirus. Both protective humoral and cellular immune responses were detected.


RESULTS
Recombinant baculovirus could express complete Env proteins and the titer of neutralizing antibody in the prime-boost group was found to be 5 to 9 folds higher than that in the rVV group.


CONCLUSIONS
Combination of protein antigen and recombinant vaccinia virus could induce high titer of neutralizing antibody against EIAV. This work facilitates development of a novel genetically engineered vaccine of EIAV as a model for future development of an HIV vaccine.
Dai
Chun-ming
CM
Division of Research on Virology and Immunology, National Center for AIDS/STD Control and Prevention, CDC, Beijing 100050, China. dcmzd@hotmail.com
Zhang
Xiao-yan
XY
Zhang
Ran-ran
RR
Shao
Yi-ming
YM
Shen
Rong-xian
RX
D000818:Animals; D000917:Antibody Formation; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D015752:Genes, env; D007111:Immunity, Cellular; D007114:Immunization; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism; D014162:Transfection; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics*
D015686:Gene Products, env; D019444:Vaccines, DNA