title: [Muscle regeneration following glycerol injection mimic that of mdx-mice degenerative-regenerative groups].
abstract: BACKGROUND
  In recent years several authors have proposed that muscle pathological changes in humans with dystrophin disorders and in mdx-mice are the result of regeneration attempts of this tissue subjected to constantly repeated bouts of necrosis.
  
  
  OBJECTIVE
  We compared morphological aspects of degenerative-regenerative groups observed in the mdx-mice with the regenerative phases that follow an intramuscular injection of 30 mL glycerol.
  
  
  METHODS
  Gastrocnemius samples from mdx-mice, 1 to 5 months old and from glycerol injected C57Bl10/ScSn mice, 4 to 8 months old, were processed and analyzed in parallel.
  
  
  RESULTS
  We were able to recognize degenerative-regenerative group stages in the mdx-mice that closely matched the anomalies found 1, 2, 3, 4-5, 7 and 21-28 days following the injection with glycerol. CONCLUSION. This study reinforces the hypothesis that muscle pathologic abnormalities of mdx-mice are the result of chronic muscle necrosis-regeneration. Morphologic mdx-mice degenerative-regenerative group staging will be a helpful tool for future investigation of muscle regeneration.
authors.affiliation: Grup de Recerca de Malalties Neurometabòliques, Hospital Universitari Materno-Infantil, Hospital Universitari Vall d'Hebron, Barcelona, Spain. manroig@cs.vhebron.es
authors.forename: M
authors.initials: M
authors.lastname: Roig-Quilis
authors.forename: J
authors.initials: J
authors.lastname: Roma
authors.forename: M
authors.initials: M
authors.lastname: Marotta
authors.forename: Y
authors.initials: Y
authors.lastname: Sarria
authors.forename: A
authors.initials: A
authors.lastname: Fargas
mesh_terms: D000818:Animals; D005260:Female; D005990:Glycerol / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000473:pathology* / Q000502:physiology; D009137:Muscular Dystrophy, Animal / Q000188:drug therapy / Q000473:pathology; D012038:Regeneration / Q000502:physiology*
chemical_list: D005990:Glycerol