title: Determination of protein-ligand affinity constants from direct migration time in capillary electrophoresis.
abstract: A simple method to calculate dissociation constants for protein-ligand interactions by partial-filling capillary electrophoresis is demonstrated. The method uses raw migration time data for the ligand and needs only additional information about capillary inner radius and the absolute amount of protein loaded. A theoretical study supported by experimental data also demonstrates that the retention of analyte in affinity capillary electrophoresis (ACE) using the partial-filling technique depends linearly on the absolute amount of selector added but is independent of both selector zone length and selector mobility. Factors such as field strength and electroosmotic flow are also cancelled out if they are kept constant. The theory is confirmed and the usefulness of the method is demonstrated by enantioseparations using alpha-acid glycoprotein (AGP) and cellulase (Cel 7A) as chiral selectors.
authors.affiliation: Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Nilsson
authors.forename: Valerie
authors.initials: V
authors.lastname: Harang
authors.forename: Maria
authors.initials: M
authors.lastname: Bergström
authors.forename: Sten
authors.initials: S
authors.lastname: Ohlson
authors.forename: Roland
authors.initials: R
authors.lastname: Isaksson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Johansson
mesh_terms: D000465:Algorithms; D019075:Electrophoresis, Capillary / Q000379:methods*; D008024:Ligands; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*
chemical_list: D008024:Ligands; D011506:Proteins