Effect of hyperoxia on interleukin-8 expression in premature versus term rabbit lung explants.
To examine whether prematurity significantly changes the lung inflammatory response to oxygen, rabbit lung explant cultures were exposed to 95% or 5% oxygen for 24 hours. Interleukin (IL)-8 protein concentrations from homogenates of the premature lung rose significantly after hyperoxia (6.8 +/- 1.8 in 5% O2 to 45.7 +/- 21.3 pg/microg protein in 95% O2) but not in the term lung (15.9 +/- 6.7 to 20.4 +/- 4.3 pg/microg protein). There was no change in IL-8 mRNA after hyperoxia in either age group. Preterm lungs demonstrated higher IL-8 levels by fluorescence-activated cell sorting (FACs) analysis and immunohistochemistry. This model may help determine why premature lungs are more susceptible to oxygen-induced disease.
Rozycki
Henry J
HJ
Department of Pediatrics, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA. hrozycki@hsc.vcu.edu
Eischeid
Anne C
AC
Huff
Thomas F
TF
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D018496:Hyperoxia / Q000235:genetics / Q000503:physiopathology*; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D011247:Pregnancy; D047928:Premature Birth / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D047929:Term Birth / Q000235:genetics* / Q000378:metabolism
D016209:Interleukin-8; D012333:RNA, Messenger; D010100:Oxygen