title: Expression of Epstein-Barr virus latent genes in oral epithelium: determinants of the pathogenesis of oral hairy leukoplakia.
abstract: This retrospective study examined expression of Epstein-Barr virus (EBV) latent genes in oral epithelium from human immunodeficiency virus-seropositive subjects, to identify genes associated with the pathogenesis of oral hairy leukoplakia (HLP). Transcription of EBV latent genes was detected in tissues with productive EBV replication and, also, in normal oral epithelial tissues without EBV replication. Expression of the EBV EBNA-2 open-reading frame in oral epithelium was identified as an important cofactor associated with the pathogenesis of HLP. In vitro experiments suggested that a recombinant variant of the EBNA-2 gene may play a role in the pathogenesis of HLP, through modulation of EBNA-2 protein function.
authors.affiliation: Division of Infectious Diseases, Department of Internal Medicine, and Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA. dwalling@utmb.edu
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Walling
authors.forename: Paul D
authors.initials: PD
authors.lastname: Ling
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Gordadze
authors.forename: Miguel
authors.initials: M
authors.lastname: Montes-Walters
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Flaitz
authors.forename: C Mark
authors.initials: CM
authors.lastname: Nichols
mesh_terms: D000212:Acyclovir / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001706:Biopsy; D004848:Epithelium / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics* / Q000502:physiology; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D015658:HIV Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D017733:Leukoplakia, Hairy / Q000188:drug therapy / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000235:genetics; D012189:Retrospective Studies; D014158:Transcription, Genetic; D000077483:Valacyclovir; D014633:Valine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins; D014779:Virus Replication
chemical_list: C402641:EBNA-2 protein, Human herpesvirus 4; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; C106732:Epstein-Barr virus encoded RNA 2; D012333:RNA, Messenger; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D014633:Valine; D000077483:Valacyclovir; C417029:EBV-encoded nuclear antigen 1; D000212:Acyclovir