Therapeutic efficacy as predicted by quantitative assessment of murine RIF-1 tumour pH and phosphorous metabolite response during hyperthermia: an in vivo 31P NMR study.
Described herein are the initial findings from an 'in-magnet' 31P NMR compatible hyperthermia system capable of concurrently heating and monitoring the metabolic response of murine tumours; the murine radiation induced fibrosarcoma (RIF-1) was employed for these studies. At thermal doses sufficient to raise tumour temperature to 41.5 and 43 degrees C for a period of 30 min, a marked and rapid decrease in nucleoside triphosphate concentration and in pH was observed during the heating period, while inorganic phosphate concentration increased significantly but more gradually. These 31P NMR determined metabolic indices remained depressed/elevated throughout a 1.5 h post-hyperthermia monitoring period. Importantly, these metabolic indices correlated significantly with specific growth delay. This suggests a possible role for NMR spectroscopy in early assessment, and perhaps control, of therapeutic response to hyperthermia.
Bezabeh
T
T
Department of Chemistry, Campus Box 1134, Washington University, One Brookings Drive, St. Louis, Missouri 63130-4899, USA.
Evelhoch
J L
JL
Thompson
P
P
Sloop
D J
DJ
Ackerman
J J H
JJ
D000818:Animals; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D005544:Forecasting; D006863:Hydrogen-Ion Concentration; D006979:Hyperthermia, Induced; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010759:Phosphorus Isotopes
D010710:Phosphates; D010759:Phosphorus Isotopes; D010758:Phosphorus