[Adenovirus-mediated gene transfer of rHSG-1 inhibits proliferation of vascular smooth muscle cells from spontaneously hypertensive rats].
OBJECTIVE
To investigate the effect of rat hyperplasia suppressor gene-1(rHSG-1) on the proliferation of aortic vascular smooth muscle cells(VSMCs) from spontaneously hypertensive rats(SHR).


METHODS
VSMCs were transfected with an adenoviral vector expressing rHSG-1(Ad5rHSG-1). The effect of rHSG-1 on the proliferation of VSMCs was investigated by cell counting, MTT assay and (3)H-thymidine incorporation. We also analyzed the cell-cycle using flow cytometry and detected the expression of p27(Kip1) and p21(Cip1) by Western Blot.


RESULTS
The proliferation of VSMCs infected with Ad5rHSG-1 was inhibited  a 40% reduction compared with the control group(P<0.01). The cell cycle was arrested in G(0)/G(1) phase and the expression of p27(Kip1) and p21(Cip1) was upregulated after the VSMCs transfection with Ad5rHSG-1.


CONCLUSIONS
Adenovirus-mediated gene transfer of rHSG-1 inhibits the cell cycle progression and thus the proliferation of VSMCs from SHR.
Li
Peng-fei
PF
Peking University Institue of Cardiovascular Sciences, Beijing 100083, China.
Guo
Yan-hong
YH
Li
Qian
Q
Yao
Peng-ying
PY
Chen
Guang-hui
GH
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis; D002455:Cell Division; D050760:Cyclin-Dependent Kinase Inhibitor p27; D022761:Gene Transfer, Horizontal; D015316:Genetic Therapy; D006965:Hyperplasia / Q000517:prevention & control*; D006973:Hypertension / Q000473:pathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000032:analysis
C493962:Cdkn1b protein, rat; D018797:Cell Cycle Proteins; D018809:Proliferating Cell Nuclear Antigen; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27