title: Identification of a novel hemotropic mycoplasma in a splenectomized dog with hemic neoplasia.
abstract: A 3-year-old sexually intact male Bull Mastiff underwent splenectomy for splenic thrombosis; prior to and after splenectomy, multiple blood transfusions were administered. Two weeks after the procedure, T-cell lymphoproliferative disease was diagnosed. Treatment with prednisone and chlorambucil was initiated, and 2 weeks later, cytologic examination of a blood smear revealed small (0.3 microm), coccoid basophilic bodies on the surface of approximately 70% of the RBCs. Morphologically, these resembled "Candidatus Mycoplasma haemominutum." A polymerase chain reaction assay was used to amplify a partial 16S rRNA sequence in blood obtained from the dog; the product was sequenced and compared with 16S rRNA gene sequences of other hemotropic mycoplasmas. The sequence was 98% homologous to that of "Candidatus M haemominutum", but only 77% homologous to that of M haemocanis and M haemofelis.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Sykes
authors.forename: Nathan L
authors.initials: NL
authors.lastname: Bailiff
authors.forename: Louise M
authors.initials: LM
authors.lastname: Ball
authors.forename: Oded
authors.initials: O
authors.lastname: Foreman
authors.forename: Jeanne W
authors.initials: JW
authors.lastname: George
authors.forename: Michael M
authors.initials: MM
authors.lastname: Fry
mesh_terms: D000743:Anemia, Hemolytic / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D004275:DNA, Ribosomal / Q000032:analysis; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D017809:Fatal Outcome; D016867:Immunocompromised Host; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007945:Leukemia, Lymphoid / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D008297:Male; D009174:Mycoplasma / Q000235:genetics* / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013156:Splenectomy / Q000662:veterinary; D013927:Thrombosis / Q000601:surgery / Q000662:veterinary
chemical_list: D004275:DNA, Ribosomal; D007166:Immunosuppressive Agents; D012336:RNA, Ribosomal, 16S