title: The sequencing-based typing tool of dbMHC: typing highly polymorphic gene sequences.
abstract: The dbMHC resource (http://www.ncbi.nlm.nih.gov/mhc/sbt.cgi?cmd=main) at the National Center for Biotechnology Information (NCBI) has developed an online tool for evaluating the allelic composition of sequencing-based typing (SBT) results of cDNA or genomic sequences. Whether the samples are heterozygous, haploid or a combination of the two, they can be compared with two up-to-date databases of all known alleles of several human leukocyte antigen (HLA) and killer cell immunoglobulin-like receptor (KIR) loci. The results of the submission are returned as a table of potential allele hits, along with the respective base changes and an interactive sequence viewer for close examination of the alignment.
authors.affiliation: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 45, 8600 Rockville Pike, Bethesda, MD 20894, USA. helmberg@ncbi.nlm.nih.gov
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Helmberg
authors.forename: Raymond
authors.initials: R
authors.lastname: Dunivin
authors.forename: Michael
authors.initials: M
authors.lastname: Feolo
mesh_terms: D000465:Algorithms; D000483:Alleles; D016172:DNA Fingerprinting; D030561:Databases, Nucleic Acid; D006680:HLA Antigens / Q000235:genetics*; D020407:Internet; D011110:Polymorphism, Genetic; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D006680:HLA Antigens; D011971:Receptors, Immunologic; D054340:Receptors, KIR