Accumulation of quinolinic acid with neuroinflammation: does it mean excitotoxicity?
The quinolinic acid (QUIN) accumulation that is associated with neuroinflammation is often considered capable of promoting excitotoxic neuronal damage, but QUIN is a relatively weak agonist of N-methyl-D-aspartate (NMDA) receptors. Our study aimed to determine, in vivo, which extracellular concentrations of QUIN must be reached to initiate electrophysiological changes indicative of excitotoxic stress in the cerebral cortex of rats, under normal conditions and when superimposed to a challenge involving NMDA-receptor activation, i.e. repeated cortical spreading depression (CSD). Our experimental strategy relied on microdialysis probes incorporating an electrode, implanted in the brain of halothane-anaesthetised rats. These devices were used to apply QUIN or NMDA locally to the cortical area under study (with or without co-perfusion of high K+ for repetitive induction of CSD), and to record the associated changes in the extracellular DC potential (for information on the membrane polarisation of the cellular population surrounding the probe) and lactate (for the detection of increased local energy demand). The extracellular EC50 for induction of local depolarisation in the normal cortex was around 30 times higher than the extracellular QUIN levels measured in the immunoactivated brain of gerbils. Within the range of concentrations 0.03 to 0.3 mM in the perfusion medium, QUIN suppressed concentration-dependently the elicitation of CSD by K+, presumably because of NMDA-receptor desensitisation. Finally, on-line monitoring of changes in extracellular lactate with local application of QUIN indicated that extracellular concentration of QUIN in the low micromolar range are well tolerated by the brain parenchyma, at least in cortical regions. All these data do not support the notion that QUIN accumulation adds an excitotoxic component to neuroinflammation.
Obrenovitch
Tiho P
TP
Pharmacology, School of Pharmacy, University of Bradford, Bradford, BD7 1DP, UK. t.obrenovitch@bradford.ac.uk
Urenjak
Jutta
J
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004660:Encephalitis / Q000209:etiology* / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate / Q000378:metabolism / Q000633:toxicity; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009498:Neurotoxins / Q000378:metabolism* / Q000633:toxicity; D011188:Potassium / Q000494:pharmacology; D017378:Quinolinic Acid / Q000378:metabolism* / Q000633:toxicity; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists
D009498:Neurotoxins; D016194:Receptors, N-Methyl-D-Aspartate; D019344:Lactic Acid; D016202:N-Methylaspartate; D017378:Quinolinic Acid; D011188:Potassium