[Effect of acrylonitrile on the spermatogenesis in mice].
OBJECTIVE
To observe the effect of acrylonitrile on the spermatogenesis in mice.


METHODS
50 male pubertal mice and 25 male adult mice were exposed to acrylonitrile (1.25, 2.5, 5 mg/kg) by intraperitoneal injection for 5 days, and detected the DNA content and cell period of spermatogenic cells by flow cytometry after 35 days.


RESULTS
(1) The percentage of 1C cell in the exposed pubertal mice groups was lower than in the control group, respectively. The percentage of 1C cell in the exposed adult mice groups was lower than in the control group, respectively (P < 0.05). The percentage of apoptotic cell in pubertal and adult mice was higher than in the control group, respectively (P < 0.05). (2) The percentage of cells in S-phase in the exposed pubertal mice groups was higher than in the control group, respectively (P < 0.05), G0/G1 phase were lower(P < 0.05). The percentage of cells in G2/M phase in the exposed adult mice groups was higher than in the control group, respectively(P < 0.05), G0/G1 phase was lower(P < 0.05).


CONCLUSIONS
There are restrained effects of acrylonitrile on the spermatogenesis in mice.
Liu
Xiaoning
X
School of Public Health, Lanzhou Medical College, Lanzhou 730000, China.
Xiao
Wei
W
Wang
Zhenquan
Z
Lian
Suqin
S
D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004247:DNA / Q000032:analysis; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects
D004247:DNA; D000181:Acrylonitrile