Embryotoxicity in vitro with extract of Indigofera suffruticosa leaves.
Aqueous extract of leaves of Indigofera suffruticosa (AELIs) were studied for adverse effects in preimplantation mouse embryos. Two-cell mouse embryos were cultured for 94 h in human tubal fluid medium (HTF), and AELIs at a concentration of 5 or 10 mg/ml. On Day 4 of culture, morulae and blastocysts were collected for morphological analysis of blastomeres. We found that embryos exposed to the higher concentration of AELIs (10 mg/ml) did not develop and all embryos persisted at the two-cell stage. Those embryos exposed to the lower concentration (5 mg/ml) showed development until morula, blastocyst and hatched blastocyst stages that were similar to the controls. These results suggest that use of AELIs may be hazardous to humans who make use of it in folk medicine.
Leite
Sônia Pereira
SP
Laboratório de Cultura de Células II, Departamento de Histologia e Embriologia do Centro de Ciências Biológicas da, Universidade Federal de Pernambuco (UFPE), Cidade Universitária, Recife 50, 670-420 Pernambuco, Brazil. spl@ufpe.br
de Medeiros
Paloma Lys
PL
da Silva
Eliete Cavalcanti
EC
de Souza Maia
Maria Bernadete
MB
de Menezes Lima
Vera Lúcia
VL
Saul
Daniel Eduardo
DE
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D005260:Female; D029883:Indigofera; D051379:Mice; D009024:Morphogenesis / Q000187:drug effects; D009028:Morula / Q000187:drug effects*; D009924:Organ Culture Techniques; D010936:Plant Extracts / Q000633:toxicity*; D018515:Plant Leaves / Q000633:toxicity*; D011247:Pregnancy
D010936:Plant Extracts