title: Ecdysone receptor-dependent gene regulation mediates histone poly(ADP-ribosyl)ation.
abstract: While the ecdysone dependency of puff formation in giant polytene chromosomes from fly salivary glands has been well documented, the molecular mechanisms underlying this process remain unknown. However, it does appear to involve chromatin remodeling and modification mediated by ecdysone receptor (EcR). As Drosophila poly(ADP-ribose) polymerase (dPARP) has recently been reported to be involved in ecdysone-induced puff formation, we decided to test the possible role of dPARP in ligand-induced dEcR transactivation in an insect system. dPARP co-activated the ligand-induced transactivation function of EcR in the insect cell line S2, and appeared to physically interact with EcR in a ligand-dependent manner. ChIP analysis of an EcR target gene promoter revealed ligand-dependent recruitment of dPARP with poly(ADP-ribosyl)ation of histones in the EcR binding site and, surprisingly, also in a distal region of the promoter. Our results indicated that EcR-mediated gene regulation may be coupled with chromatin modification through poly(ADP-ribosyl)ation.
authors.affiliation: The Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Sawatsubashi
authors.forename: Akio
authors.initials: A
authors.lastname: Maki
authors.forename: Saya
authors.initials: S
authors.lastname: Ito
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shirode
authors.forename: Eriko
authors.initials: E
authors.lastname: Suzuki
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Kaoru
authors.initials: K
authors.lastname: Yamagata
authors.forename: Alexander
authors.initials: A
authors.lastname: Kouzmenko
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Takeyama
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000196:embryology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006657:Histones / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*
chemical_list: D006657:Histones; D011987:Receptors, Steroid; C055469:ecdysone receptor; C032622:poly(ADP-ribosylated) histone H1; D011065:Poly(ADP-ribose) Polymerases