Phospholipase-mediated preparation of 1-ricinoleoyl-2-acyl-sn-glycero-3-phosphocholine from soya and egg phosphatidylcholine.
1-Ricinoleoyl-2-acyl-sn-glycero-3-phosphocholine was prepared by incorporating ricinoleic acid completely in the sn-1 position of egg and soya phosphatidylcholine (PC) using immobilized phospholipase A(1) as the catalyst. The optimum reaction conditions for maximum incorporation of ricinoleic acid into PC through transesterification were 10% (w/w) immobilized enzyme (116 mg), a 1:5 mol ratio of PC (soya, 387 mg; egg, 384 mg) to methyl ricinoleate (780 mg) at 50 degrees C for 24 h in hexane.
Vijeeta
T
T
Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad-500 007, India.
Reddy
J R C
JR
Rao
B V S K
BV
Karuna
M S L
MS
Prasad
R B N
RB
D004531:Eggs / Q000032:analysis*; D010713:Phosphatidylcholines / Q000138:chemical synthesis*; D010741:Phospholipases A / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
D010713:Phosphatidylcholines; D010741:Phospholipases A