title: Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1.
abstract: We performed high-throughput in situ hybridization screening of sections of mouse epidermis using an equalized skin cDNA library as probes and identified a novel gene giving rise to two splicing variants, both of which are expressed in the spinous layer. This gene was mapped between two genes encoding keratinocyte-related peptides, suprabasin and keratinocyte differentiation-associated protein (Kdap), on human chromosome 19q13.1. These gene products appeared to carry functional signal sequences. We then designated these two splicing variants as dermokine-alpha and -beta. Northern blotting and quantitative RT-PCR revealed that dermokine-alpha/-beta, suprabasin, and Kdap were highly expressed in stratified epithelia. In mouse embryonic development, dermokine-alpha/-beta began to be expressed during the period of stratification. Also, in differentiating primary cultured human keratinocytes, transcription of dermokine-alpha/-beta, suprabasin, and Kdap was induced. These findings indicated that dermokine-alpha/-beta, suprabasin, and Kdap are secreted from the spinous layer of the stratified epithelia and that these genes form a novel gene complex on the chromosome.
authors.affiliation: KAN Research Institute, Inc., Shimogyo-ku, Kyoto 600-8815, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsui
authors.forename: Fumie
authors.initials: F
authors.lastname: Hayashi-Kisumi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Sayaka
authors.initials: S
authors.lastname: Katahira
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Morita
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Miyachi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ono
authors.forename: Toshio
authors.initials: T
authors.lastname: Imai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tanigawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Komiya
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D003001:Cloning, Molecular; D004817:Epidermis / Q000378:metabolism; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D000943:Antigens, Differentiation; C495568:DMKN protein, human; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; C495569:dermokine protein, mouse; C470006:suprabasin protein, mouse; D016282:Aspartic Acid Endopeptidases; C104073:Kdap protein, mouse