title: Role of testicular tissue telomerase assay for the prediction of the presence of testicular spermatozoa in azoospermic men with varicoceles, pre- and post-varicocelectomy.
abstract: We evaluated the reproductive potential of frozen/thawed testicular spermatozoa of azoospermic men with left varicocele. The role of testicular tissue telomerase assay (TTA) in the prediction of the presence of testicular spermatozoa pre- and post-varicocelectomy was investigated, as well. Therapeutic testicular biopsy and TTA were performed in 82 nonobstructed azoospermic (NOA) men with varicoceles. Testicular spermatozoa were found in 33 men and processed for cryopreservation. Oocytes were later recovered from the spouses of the latter azoospermic men with varicoceles and injected with frozen/thawed testicular spermatozoa. Among the 49 men who were negative for testicular spermatozoa, 22 men underwent subsequently subinguinal microsurgical varicocelectomy. A total of 198 mature oocytes were successfully injected and 101 were normally fertilized and subsequently cleaved. Transfer of these 101 embryos in 26 women resulted in nine full-term pregnancies. Thirteen healthy babies were delivered. A cut-off value of TTA of 39 TPG U microg(-1) protein had an overall diagnostic accuracy equal to 90.2% to predict the presence of testicular spermatozoa pre-varicocelectomy. Within the group of men who were negative for testicular spermatozoa a cut-off value of TTA equal to 28 TPG U microg(-1) protein (pre-varicocelectomy) had a 84.2 % diagnostic accuracy to recognize the men who would become positive for either ejaculated or testicular spermatozoa post-varicocelectomy. Testicular spermatozoa can be found in 40% of NOA men with left varicocele. Ooplasmic injections with frozen/thawed testicular spermatozoa have a role in the therapeutic management of non-obstructive azoospermia associated with varicocele. Pre-varicocelectomy, a TTA cut-off value equal to 39 TPG U microg(-1) protein has a 90.2% diagnostic accuracy to indicate the men positive/negative for testicular spermatozoa. In addition, pre-varicocelectomy, a cut-off value equal to 28 TPG U microg(-1) protein has a 84.2% diagnostic accuracy to identify those men with varicoceles without testicular spermatozoa, who will become positive/negative for spermatozoa (either ejaculated or testicular) post-varicocelectomy.
authors.affiliation: Laboratory of Molecular Urology and Genetics of Human Reproduction, Department of Urology, Ioannina University School of Medicine, Ioannina, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Giannakis
authors.forename: D
authors.initials: D
authors.lastname: Baltogiannis
authors.forename: K
authors.initials: K
authors.lastname: Tsoukanelis
authors.forename: D
authors.initials: D
authors.lastname: Loutradis
authors.forename: I
authors.initials: I
authors.lastname: Miyagawa
authors.forename: G
authors.initials: G
authors.lastname: Makrydimas
authors.forename: N
authors.initials: N
authors.lastname: Kanakas
authors.forename: N
authors.initials: N
authors.lastname: Sofikitis
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004542:Ejaculation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000150:complications* / Q000503:physiopathology*; D011184:Postoperative Period; D011237:Predictive Value of Tests; D011300:Preoperative Care; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D013091:Spermatogenesis; D013094:Spermatozoa; D019098:Telomerase / Q000378:metabolism*; D013737:Testis / Q000201:enzymology*; D020858:Tissue and Organ Harvesting; D014646:Varicocele / Q000150:complications* / Q000601:surgery*
chemical_list: D019098:Telomerase