Prediction of antibiotic activity and synthesis of new pentadecapeptides based on lactoferricins.
The antibacterial activity against Escherichia coli and Staphylococcus aureus has been studied for a number of modified pentadecapeptides based on lactoferricins of different origin. The peptides were classified by multivariate methods and quantitative structure-activity relationships (QSAR) were developed using theoretically derived variables for the amino acids. For the modified peptides based on bovine lactoferricin (LFB) a model was calculated and used for prediction of new peptides that were then tested for antibacterial activity in order to improve peptide activity and to check the validity of the model. Models were also calculated including lactoferricins of different origin. Theories of the mechanism of action of the peptides are briefly discussed.
Lejon
Tore
T
Department of Chemistry, University of Tromsø, N-9037 Tromsø, Norway. tore.lejon@chem.uit.no
Stiberg
Trine
T
Strøm
Morten B
MB
Svendsen
John S
JS
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D019295:Computational Biology; D004926:Escherichia coli / Q000187:drug effects; D007781:Lactoferrin / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides; D021281:Quantitative Structure-Activity Relationship; D012984:Software; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D010455:Peptides; D007781:Lactoferrin