Detection of DNA hybridization on a liposome surface using ultrasound velocimetry and turbidimetry methods.
19-mer oligonucleotides with oleylamine tethered at 3' and 5' terminal, respectively, were incorporated into unilamellar liposomes of dioleoylphosphatidylcholine (DOPC). Addition of complementary nucleotide resulted in hybridization with oligonucleotides located on different liposomes and caused liposome aggregation. Significant changes of sound velocimetry and turbidity were readily observed at 10 nM concentration of the complementary chain.
Hianik
Tibor
T
Department of Biophysics and Chemical Physics, Comenius University, 842 48 Bratislava, Slovakia. Hianik@fmph.uniba.sk
Rybar
Peter
P
Andreev
Sergej Yu
SY
Oretskaya
Tatiana S
TS
Vadgama
Pankaj
P
D001483:Base Sequence; D011088:DNA Ligases; D004279:DNA, Viral / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013499:Surface Properties
D004279:DNA, Viral; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D011088:DNA Ligases