title: Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing.
abstract: In order to investigate the genotypes of Giardia duodenalis from domestic and farm animals in Italy, 21 Giardia isolates, 17 from dogs, 1 from cat and 3 from dairy calves, were genetically characterised by SSU-rRNA gene sequencing. Among dogs, 76.5% of isolates showed the dog-specific genotypes (Assemblages C, D and C/D mixed Assemblage) and 23.5% exhibit potential zoonotic genotypes (Assemblage A and A/C mixed Assemblages). The cat isolate belonged to assemblage A, whereas the sequences among the isolates from calves were found to correspond to hoofed-livestock genotype, namely Assemblage E. These findings suggest that infection of humans by zoonotic genotypes from domestic animals could be of low epidemiological significance, although possible. The present study represents the first contribute to the knowledge of G. duodenalis genotypes in domestic and farm animals from Italy.
authors.affiliation: Cattedra di Parassitologia, Dipartimento di Sanità Pubblica e Biologia Cellulare, Università di Roma Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. berrilli@uniroma2.it
authors.forename: Federica
authors.initials: F
authors.lastname: Berrilli
authors.forename: David
authors.initials: D
authors.lastname: Di Cave
authors.forename: Claudio
authors.initials: C
authors.lastname: De Liberato
authors.forename: Alessia
authors.initials: A
authors.lastname: Franco
authors.forename: Paola
authors.initials: P
authors.lastname: Scaramozzino
authors.forename: Paola
authors.initials: P
authors.lastname: Orecchia
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D007558:Italy / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D012336:RNA, Ribosomal, 16S