title: Comparing the osteogenic capacities of bone substitutes: hydroxyapatite, high-density porous polyethylene, and bone collagen: a biochemical and histological analysis.
abstract: Several inorganic materials have been shown previously to hold some osteogenic capacity. The purpose of this study is to compare the bone-forming abilities of hydroxyapatite ceramic, high-density porous polyethylene, and bone collagen within the periosteal island flap of rabbit tibia using histological and biochemical analysis. With this goal, four discrete experimental groups were formed, each comprising 22 New Zealand male rabbits. A sac was created on each rabbit tibial periosteum flap in each of the groups, and each of the previously mentioned materials was placed within this sac separately. One of these groups was thought as a control group without any material being placed inside the periosteal sac. Biopsies were taken at weeks 1, 2, 4, and 8 for biochemical analysis and at weeks 2 and 8 for histological evaluation. Neo-osteogenesis was evaluated quantitatively by determination of alkaline phosphatase and osteocalcin levels biochemically as well as by the percentage of new bone formation inside the periosteal sac histologically. Results show statistically that the osteogenic effect of high-density porous polyethylene is greater than that of the other materials used in this study (P < 0.05).
authors.affiliation: Department of Plastic and Reconstructive Surgery, Ege University, Izmir, Turkey. ubilkay@hotmail.com
authors.forename: Ufuk
authors.initials: U
authors.lastname: Bilkay
authors.forename: Mehmet
authors.initials: M
authors.lastname: Alper
authors.forename: Naci
authors.initials: N
authors.lastname: Celik
authors.forename: Ozgur
authors.initials: O
authors.lastname: Erdem
authors.forename: Hakan
authors.initials: H
authors.lastname: Kerem
authors.forename: Cuneyt
authors.initials: C
authors.lastname: Ozek
authors.forename: Osman
authors.initials: O
authors.lastname: Zekioglu
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Delen
authors.forename: Ecmel
authors.initials: E
authors.lastname: Songur
authors.forename: Arman
authors.initials: A
authors.lastname: Cagdas
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001706:Biopsy; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000494:pharmacology*; D002516:Ceramics; D003094:Collagen / Q000502:physiology; D006882:Hydroxyapatites / Q000494:pharmacology; D008297:Male; D015675:Osteocalcin / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D010521:Periosteum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020959:Polyethylene / Q000494:pharmacology; D011817:Rabbits; D013524:Surgical Flaps / Q000009:adverse effects / Q000473:pathology; D013977:Tibia; D014946:Wound Infection / Q000209:etiology
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D015675:Osteocalcin; D020959:Polyethylene; D003094:Collagen