title: The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state.
abstract: The development of multi-parameter flow cytometric techniques in our laboratories has led to a functional classification of the physiological state of single celled micro-organisms, including both yeast and bacteria. This classification is based on the presence or absence of an intact fully polarized cytoplasmic membrane and the transport systems across it. Using these techniques it is possible to resolve a cells physiological state, beyond culturability to include metabolic activity enabling assessment of population heterogeneity. Importantly results are available in real-time, 1-2 min after a sample is taken, enabling informed decisions to be taken about a process. These techniques have been extensively applied by us for monitoring the stress responses of micro-organisms in such diverse areas as brewing, bio-remediation, bio-transformation, food processing and pharmaceutical fermentation, some of which are discussed here.
authors.affiliation: Centre for Formulation Engineering, Biochemical Engineering, School of Engineering (Chemical Engineering), The University of Birmingham, Edgbaston B15 2TT, UK. c.j.hewitt@bham.ac.uk
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hewitt
authors.forename: Gerhard
authors.initials: G
authors.lastname: Nebe-Von-Caron
mesh_terms: D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D018869:Heat-Shock Response / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009862:Online Systems
chemical_list: D001426:Bacterial Proteins