title: Structural and biological characterization of two novel peptides from the venom of the neotropical social wasp Agelaia pallipes pallipes.
abstract: The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences: Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH(2). Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH(2). Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL.
authors.affiliation: CEIS-Department of Biology, IBRC-UNESP (CAT-CEPID, FAPESP), Institute of Immunological Investigations (Millennium Institute-MCT, CNPq), Rio Claro, SP 13506-900, Brazil.
authors.forename: Maria Anita
authors.initials: MA
authors.lastname: Mendes
authors.forename: Bibiana Monson
authors.initials: BM
authors.lastname: de Souza
authors.forename: Mauricio Ribeiro
authors.initials: MR
authors.lastname: Marques
authors.forename: Mario Sergio
authors.initials: MS
authors.lastname: Palma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001938:Brazil; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002630:Chemotactic Factors / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D036341:Intercellular Signaling Peptides and Proteins; D008407:Mast Cells / Q000378:metabolism; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009943:Organophosphorus Compounds; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D014862:Wasp Venoms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014863:Wasps / Q000737:chemistry*
chemical_list: C043517:2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; D000900:Anti-Bacterial Agents; D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; D009943:Organophosphorus Compounds; D010455:Peptides; D014862:Wasp Venoms; C554058:protonectin (1-6), Agelaia pallipes pallipes; C037500:mastoparan