[Effect of cigarette smoke solution on viability of rat lymphocytes and the intervention of antioxidants].
OBJECTIVE
To learn the effect of cigarette smoke solution (CSS) on viability of rat lymphocytes and the intervention of antioxidants.


METHODS
CSS was prepared by bubbling main-stream cigarette smoke into PBS, then 0.1 x 10(-3) cig/ml, 2 x 10(-3) cig/ml, 4 x 10(-3) cig/ml, 8 x 10(-3) cig/ml, 12 x 10(-3) cig/ml, 16 x 10(-3) cig/ml CSS were added into 7 groups of rat lymphocytes respectively. Alamar blue was added to each group 2 hours later, and after another 4 hours reduction rate of Alamar blue was detected and used to evaluate viability of cells. Otherwise, pretreated with vitamin C (VC), vitamin E(VE), glutathione(GSH), melatonin(MLT) or dimethyl sulfoxide(DMSO), cells were treated with 8 x 10(-3) cig/ml CSS and reduction rates of Alamar blue were detected as before.


RESULTS
Alamar blue reduction rates in 2 x 10(-3) cig/ml - 16 x 10(-3) cig/ml groups were lower than that of control significantly, and there was a dose-effect relationship. Also, Alamar blue reduction rates increased in CSS-groups pretreated with 0.05 mmol/LVC, 0.2 mmol/LVE, 0.1 mmol/LMLT, 1 mmol/LGSH or 1% DMSO.


CONCLUSIONS
Viability of rat lymphocytes decreased in the presence of CSS, and it could be antagonised by antioxidants such as vitamin C, vitamin E, glutathione, melatonin and dimethyl sulfoxide.
Liu
Shan
S
School of Public Health, China Medical University, Shenyang 110001, China.
Sun
Guifan
G
Wan
Chao
C
Sun
Xiance
X
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D008550:Melatonin; D001205:Ascorbic Acid; D004121:Dimethyl Sulfoxide