Strand transfer to the 5' part of a tRNA as a mechanism for retrovirus patch-repair recombination in vivo.
By screening for marker-cassette deletion mutants of a murine leukaemia virus-based replication-competent vector, two occurrences of tRNA sequence patch insertions were identified. In one of the cases, 28 nucleotides from the 5' end of tRNA(Lys4) were inserted in the plus-strand orientation, which points to a novel strand-transfer mechanism to tRNAs during reverse transcriptase-mediated retroviral recombination.
Carrasco
María L
ML
Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
Duch
Mogens
M
Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
Pedersen
Finn Skou
FS
Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark.|Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
D016475:3T3 Cells; D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017930:Genes, Reporter; D051379:Mice; D008969:Molecular Sequence Data; D012357:RNA, Transfer, Lys / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D015183:Restriction Mapping
D020121:5' Untranslated Regions; D012357:RNA, Transfer, Lys; D012367:RNA, Viral