Specific PCR identification of Pasteurella multocida based on putative transcriptional regulator genes.
Pasteurella multocida is an important animal pathogen that may also infect humans through animal bites and scratches. After comparison of transcriptional regulator gene sequences from the P. multocida genome with other DNA sequences at GenBank, we identified two genes (i.e., Pm0762 and Pm1231) uniquely present in P. multocida. By using oligonucleotide primers (Pm0762F/R and Pm1231F/R) designed from these genes in PCR, it was found that specific DNA products of expected sizes were obtained with genomic DNA from P. multocida only, but not from other bacteria. These results indicated that the putative transcriptional regulator genes Pm0762 and Pm1231 are species-specific, and that the PCR methods targeting these genes provide a useful means of rapidly and precisely identifying P. multocida from other bacteria. Further elucidation of the roles and functions of these putative transcriptional regulator genes (Pm0762 and Pm1231) and their protein products may help provide valuable insight into the molecular mechanism of P. multocida virulence and pathogenicity.
Liu
Dongyou
D
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762, USA. liu@cvm.msstate.edu
Lawrence
Mark L
ML
Austin
Frank W
FW
D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006801:Humans; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial