title: Bound ferulic acid from bran is more bioavailable than the free compound in rat.
abstract: Ferulic acid (FA) is reported as a good antioxidant absorbed by human or rat but only few data deal with the influence of the food matrix on its bioavailability and with its potential protection against cardiovascular diseases and cancer. Wheat bran is used as a source of ferulic acid, the compound being mainly bound to arabinoxylans of the plant cell walls. Pharmacokinetic profiles of FA and its metabolites are established in rats. Free and conjugated FA quickly appear in plasma, reach a plateau 1 h after intake and remain approximately constant at 1 microM up to 24 h. 2.3% of FA are eliminated in urine. Compared with results obtained after intake of free FA, the presence of FA-arabinoxylans bonds in the food matrix increases the occurrence time of FA in the organism and decreases the level of urinary excretion in 24 h. Nevertheless, sulfated FA is still the main plasmatic form. The antioxidant activity of plasmas of rats fed with a standard diet (containing no FA), pure ferulic acid (5.15 mg FA/kg bw) or bran (4.04 mg FA/kg bw) are measured in an ex vivo test using AAPH as free radical inducer. Plasmas of rats fed with bran show a better antioxidant activity than the control group and the pure FA supplemented group, increasing the resistance of erythrocytes to hemolysis by factors of 2 and 1.5, respectively. These results show the good bioavailability of FA from bran and its potential efficiency to protect organism against pathology involving radical steps of development.
authors.affiliation: Département Science de l'Aliment, Laboratoire de Chimie des Substances Naturelles: Antioxydants, Arômes, Colorants, 1 avenue des Olympiades, 91744 Massy Cedex, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Rondini
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Peyrat-Maillard
authors.forename: Agnès
authors.initials: A
authors.lastname: Marsset-Baglieri
authors.forename: Gilles
authors.initials: G
authors.lastname: Fromentin
authors.forename: Philippe
authors.initials: P
authors.lastname: Durand
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.forename: Michel
authors.initials: M
authors.lastname: Prost
authors.forename: Claudette
authors.initials: C
authors.lastname: Berset
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D004043:Dietary Fiber / Q000032:analysis*; D020719:Glucuronides / Q000097:blood / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000097:blood / Q000652:urine
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D004043:Dietary Fiber; D020719:Glucuronides; D013431:Sulfates; C004999:ferulic acid