Origin of rice protein hydrolysates added to protein-free media alters secretion and extracellular proteolysis of recombinant interferon-gamma as well as CHO-320 cell growth.
CHO-320 cells, cultivated in suspension in a protein-free medium supplemented with rice protein hydrolysates (peptones), secrete recombinant interferon-gamma (IFN-gamma) that undergo will or will not proteolysis, depending on the origin of the peptones. This proteolytic event, as well as the appearance of an unidentified 70 kDa gelatinase-like protease, are attributed to a cysteine protease. Casein zymographies revealed that one rice protein hydrolysate, but not another, contains a papain-like cysteine protease whose activity is undetectable in solution. This work underlines the significance of the origin of peptones when considered as supplements in serum- and protein-free media for overproduction of recombinant proteins.
Mols
J
J
Laboratoire de Biochimie cellulaire, Place Louis Pasteur, 1., Belgium.
Peeters-Joris
C
C
Agathos
S N
SN
Schneider
Y-J
YJ
D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D006868:Hydrolysis; D007371:Interferon-gamma / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D010940:Plant Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma