title: Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method.
abstract: We, for the first time, analyzed the binding motifs of immunoglobulin G (IgG) in the cerebrospinal fluid (CSF) of human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients with a phage library displaying 12-mer random peptides. As a result, the sequences highly homologous to HTLV-I gp46 192-199, 237-243 and 255-261 were the common linear epitopes of high affinity- IgG exclusively detected in both CSF and sera of the patients. These IgG responses were confirmed with corresponding HTLV-I peptides and serum antibody titers significantly correlated with disease severity or duration. Gp46 237-243 has not been detected in previous enzyme-linked immunosorbent assay (ELISA) studies using bound longer peptides, suggesting the usefulness of the phage display method.
authors.affiliation: Department of Neurology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. fujimori@em.neurol.med.tohoku.ac.jp
authors.forename: J
authors.initials: J
authors.lastname: Fujimori
authors.forename: I
authors.initials: I
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Fujihara
authors.forename: J
authors.initials: J
authors.lastname: Feng
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Begum
authors.forename: Y
authors.initials: Y
authors.lastname: Sagara
authors.forename: H
authors.initials: H
authors.lastname: Shiraki
authors.forename: Y
authors.initials: Y
authors.lastname: Shiga
authors.forename: J
authors.initials: J
authors.lastname: Onodera
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Takase
authors.forename: M
authors.initials: M
authors.lastname: Asano
authors.forename: M
authors.initials: M
authors.lastname: Endo
authors.forename: Y
authors.initials: Y
authors.lastname: Itoyama
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D015481:HTLV-I Antibodies / Q000134:cerebrospinal fluid* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000276:immunology*; D019151:Peptide Library; D017385:Sequence Homology
chemical_list: D000939:Epitopes; D015481:HTLV-I Antibodies; D007074:Immunoglobulin G; D019151:Peptide Library