title: Neutralizing antibodies against endogenous interferon in myasthenia gravis patients.
abstract: Anti-cytokine antibodies (Abs) play an important role in the regulation of the immune response, both under normal conditions and in several autoimmune and neoplastic disorders. In the present study, we have investigated the occurrence and the clinical significance of natural neutralizing Abs (NAbs) against interferons (IFNs) alpha, beta, and gamma, as detected by bioassay, in 52 patients with myasthenia gravis (MG), and 43 sex- and age-matched healthy individuals. Patients showing titres > or = 1.3 Log t(1/10), confirmed in 2 consecutive samples collected two months apart, were considered positive. NAbs against any of the IFNs were not detected in healthy subjects. Of the 52 MG patients, 11 (21.1%) had NAbs against IFNalpha and three (5.8%) had NAbs against IFNbeta. None of these patients was found to be positive for NAbs against IFNgamma. Of the patients positive for NAbs against IFNalpha, eight (15.4%) had NAbs at titres > or =2 Log t(1/10). A positive association was observed between high titres of NAbs and the presence of thymoma. These data suggest the presence of a generalized activation of the humoral response in MG.
authors.affiliation: Department of Neurological Sciences, La Sapienza University, 00185 Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bagnato
authors.forename: Alessandro
authors.initials: A
authors.lastname: Clemenzi
authors.forename: Carolina
authors.initials: C
authors.lastname: Scagnolari
authors.forename: Luigi
authors.initials: L
authors.lastname: Strizzi
authors.forename: Antonella
authors.initials: A
authors.lastname: Di Pasquale
authors.forename: Francesca
authors.initials: F
authors.lastname: Bellomi
authors.forename: Paola
authors.initials: P
authors.lastname: Di Marco
authors.forename: Guido
authors.initials: G
authors.lastname: Antonelli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Antonini
mesh_terms: D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology; D001681:Biological Assay; D005260:Female; D006801:Humans; D007372:Interferons / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009157:Myasthenia Gravis / Q000097:blood / Q000150:complications / Q000276:immunology*; D013945:Thymoma / Q000097:blood / Q000150:complications / Q000276:immunology*; D013953:Thymus Neoplasms / Q000097:blood / Q000150:complications / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007372:Interferons