2'-Functionalized nucleic acids as structural tools in molecular biology.
Modified oligonucleotides bearing 2'-reactive groups or 2'-conjugated molecules have found wide application as structural tools in molecular biology. Of principal interest has been the use of 2'-reactive oligonucleotides for cross-linking with biomolecules and of 2'-conjugated oligonucleotides in hybridization assays. In this review we compare a range of electrophilic, nucleophilic and photoreactive groups for cross-linking and conjugation studies.
Zatsepin
Timofei S
TS
Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie gory, Moscow 119992, Russia.
Gait
Michael J
MJ
Oretskaya
Tatiana S
TS
D003432:Cross-Linking Reagents / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*
D003432:Cross-Linking Reagents; D015342:DNA Probes; D009841:Oligonucleotides; D012313:RNA