title: Determination of residues of propamocarb in wine by liquid chromatography-electrospray mass spectrometry with direct injection.
abstract: A simple, sensitive and reliable liquid chromatography-mass spectrometry method with direct injection of diluted samples is reported for the determination of propamocarb residues in wine. Red and white wines were diluted 40- and 20-fold, respectively, using water. Liquid chromatography was performed with a mobile-phase gradient and detection was by electrospray mass spectrometry in a positive ionization mode. Propamocarb was detected as the protonated molecular species at m/z 189. Using matrix-matched calibrant solutions, a calibrated range equivalent to 0.05-2.0 mg kg(-1) in red and white wines and limits of detection of 0.025 mg kg(-1) for white wine and of 0.05 mg kg(-1) for red wine (0.00125 microg ml(-1) of sample solution injected) were readily achievable. Recovery of propamocarb hydrochloride from wine spiked before dilution was in the range 91-115%. The chromatograms were free of isobaric interferences. In a small wine survey (72 samples), no residues of propamocarb were detected above 0.1 mg kg(-1).
authors.affiliation: Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK.
authors.forename: J C
authors.initials: JC
authors.lastname: Taylor
authors.forename: S J
authors.initials: SJ
authors.lastname: Hird
authors.forename: M D
authors.initials: MD
authors.lastname: Sykes
authors.forename: J R
authors.initials: JR
authors.lastname: Startin
mesh_terms: D002219:Carbamates / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis*; D006801:Humans; D010573:Pesticide Residues / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D002219:Carbamates; D005659:Fungicides, Industrial; D010573:Pesticide Residues; C033205:propamocarb