Elevation of urinary betaig-h3, transforming growth factor-beta-induced protein in patients with type 2 diabetes and nephropathy.
Transforming growth factor-beta (TGF-beta) is a pro-sclerotic growth factor implicated in the pathogenesis of diabetic nephropathy. betaig-h3 is an extracellular matrix protein which is induced in many cells by TGF-beta. This study examined urinary betaig-h3 excretion in diabetic patients with elevated urinary albumin excretion and the clinical application of urinary betaig-h3 as a marker of diabetic nephropathy. Urinary and serum betaig-h3 levels were determined by enzyme-linked immunosorbent assay in 163 type 2 diabetic patients and 101 healthy control subjects of comparable age and weight. The ratio of urinary betaig-h3 and TGF-beta to creatinine was analyzed in patients with different degree of nephropathy. The betaig-h3 to creatinine ratio in urine was elevated in all groups of type 2 diabetics with normoalbuminuria (101.6 +/- 9.27), microalbuminuria (120.2 +/- 14.48), and overt proteinuria (146.3 +/- 16.34), when compared with control subjects (64.8 +/- 7.14) (P < 0.01). There was a positive correlation between urinary betaig-h3 and TGF-beta excretion rate and a positive correlation between urinary betaig-h3 and albumin excretion rate (AER). These data show that urinary levels of betaig-h3 are elevated in type 2 diabetic patients with nephropathy and may be used as a marker of diabetic nephropathy.
Ha
Sung-Woo
SW
Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
Kim
Hyun-Ji
HJ
Bae
Jong-Sup
JS
Jeong
Gui-Hwa
GH
Chung
Sung-Chang
SC
Kim
Jung-Guk
JG
Park
Sun-Hee
SH
Kim
Yong-Lim
YL
Kam
Sin
S
Kim
In-San
IS
Kim
Bo-Wan
BW
D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000652:urine; D001786:Blood Glucose / Q000032:analysis; D003404:Creatinine / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000652:urine*; D003928:Diabetic Nephropathies / Q000097:blood / Q000652:urine*; D016326:Extracellular Matrix Proteins / Q000097:blood* / Q000652:urine*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000652:urine*; D000099222:betaIG-H3 Protein
D015415:Biomarkers; D001786:Blood Glucose; D003404:Creatinine; D016326:Extracellular Matrix Proteins; D007328:Insulin; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein