title: Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.
abstract: The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20 kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His(5) tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 A. A complete 2.0 A data set has been collected at a rotating-anode X-ray source and structure determination is under way.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.forename: Yuhe
authors.initials: Y
authors.lastname: Liang
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Haitao
authors.initials: H
authors.lastname: Ding
authors.forename: Shihong
authors.initials: S
authors.lastname: Qiu
authors.forename: Shanyun
authors.initials: S
authors.lastname: Lu
authors.forename: Jianli
authors.initials: J
authors.lastname: An
authors.forename: Lanfen
authors.initials: L
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zheng
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry*; D002853:Chromatography, Liquid; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID
chemical_list: D011506:Proteins; C106849:TAF9 protein, human; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID