title: Short-term effects of external and internal biliary drainage on liver and cellular immunity in experimental obstructive jaundice.
abstract: OBJECTIVE
  The effects of preoperative biliary drainage for obstructive jaundiced patients are controversial. Although experimental studies have proven the benefit of internal biliary drainage (ID) over external biliary drainage (ED), ID has several clinical problems, such as clogging or tube replacement. The aim of this study was to determine whether there were any differences in T-cell function, liver function, and histology, between rats in ID and ED groups in short-term experiments.
  
  
  METHODS
  Following bile duct ligation (BDL) for 14 days, rats in the ED and ID groups had 7 days of ED and 7 days of ID, respectively. Normal rats were used as negative controls (control group). For positive controls, we used a group with BDL and no drainage (BDL group). Serum bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were measured, splenic T-cell proliferation was assayed to check cellular immunity, and liver histology was examined.
  
  
  RESULTS
  Recovery of bilirubin and ALT was similar in the ED and ID groups. Recovery of AST was worse in the ID group than in the ED group, but the difference was not statistically significant. Levels of ALP in the BDL and ID groups were significantly higher than those in the control and ED groups. Rats in the BDL group showed a significant decrease in T-cell function compared to the control group. The ED group showed better recovery of T-cell function than the ID group in the 7 days after relief of obstructive jaundice. The livers in the ID group demonstrated histologically moderate interface hepatitis with periportal inflammation and lymphocyte infiltration, which strongly suggested incomplete tube obstruction, but those in the ED group showed minimal change.
  
  
  CONCLUSIONS
  ED is superior to ID concerning the recovery of cellular immunity and liver inflammation in the short-term after relief from biliary obstruction in this model. As the patency of the tube is well maintained in ED compared to ID, patency of the tube is essential to obtain good recovery of cellular immunity, irrespective of the drainage method.
authors.affiliation: Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Mizuguchi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ajiki
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Onoyama
authors.forename: Masao
authors.initials: M
authors.lastname: Tomita
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004322:Drainage / Q000379:methods*; D007111:Immunity, Cellular; D041781:Jaundice, Obstructive / Q000276:immunology* / Q000601:surgery*; D008099:Liver / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase