Determination of 14C residue in eggs of laying hens administered orally with [14C] sulfaquinoxaline.
Ten layer hens were dosed for 5 consecutive days with 6.2 mg kg(-1) [14C] sulfaquinoxaline (SQX). Eggs were collected from the hens during the 5-day dosing period and during a 10-day post-dose withdrawal period. Egg yolk and albumen were separated and assayed for total radioactive residues (TRR) using a combustion oxidizer and liquid scintillation counting techniques. Significant amounts of radioactivity were detected on the second day of dosing (greater than 24h after the initial dose) in both egg yolk and albumen. First eggs were collected about 8 h after dosing; the second-day eggs were collected during 8-h period after the second dose. Radioactive residues reached a maximum on the fifth day of dosing in albumen, whereas on the second day of withdrawal in egg yolk, the peak TRR levels in albumen were about threefold higher than in yolk. Thereafter, the TRR levels declined rapidly in albumen and were detectable up to withdrawal day 6, whereas the TRR levels in egg yolk declined more slowly and were detectable up to withdrawal day 10. High-performance liquid chromatography analysis indicated that the parent drug sulfaquinoxaline was the major component in both the egg albumen and yolk. Additionally, this work suggests that egg yolk is the appropriate matrix for monitoring SQX residues
Shaikh
B
B
FDA/CVM Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA. BSHAIKH@CVM.FDA.GOV
Rummel
N
N
Smith
D
D
D000284:Administration, Oral; D000818:Animals; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D002250:Carbon Radioisotopes; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000493:pharmacokinetics; D004529:Egg White / Q000032:analysis; D004530:Egg Yolk / Q000378:metabolism; D004531:Eggs / Q000032:analysis*; D005243:Feces / Q000737:chemistry; D005260:Female; D005506:Food Contamination / Q000032:analysis; D015813:Substance Abuse Detection / Q000379:methods / Q000662:veterinary*; D013428:Sulfaquinoxaline / Q000493:pharmacokinetics*
D000890:Anti-Infective Agents; D002250:Carbon Radioisotopes; D013428:Sulfaquinoxaline