title: A comparison of commercial kit methods for assay of vitamin b(12) in ruminant blood.
abstract: Five commercially available radioisotope dilution (RID) kits for assay of Vitamin B(12) were obtained for evaluation with ovine and bovine serum; also, for comparison with our laboratory's (Lab 1) RID method and the methods of two independent laboratories, one using RID and the other a microbiological method. One commercial kit was found to be suitable for both species because negligible non-specific binding occurred when extraction was performed at 100 degrees C (212 degrees F). Of the other four kits, two were found to be suitable for ovine and bovine serum provided non-specific binding of the latter was measured. This was also the case with the Lab 1 method. Two of the commercial kits were found to be unsuitable for assay of Vitamin B(12)in ruminant blood.
authors.affiliation: Central Veterinary Laboratories, Department of Agriculture, I.M.V.S., Frome Road, Adelaide, South Australia 5000.
authors.forename: W J
authors.initials: WJ
authors.lastname: Schultz