No evidence for amplification of 25-hydroxyvitamin D-1alpha-OHase (1alpha-OHase) or 1,25-dihydroxyvitamin D-24-OHase (24-OHase) genes in malignant melanoma (MM).
Increasing evidence points at an important function of Vitamin D metabolites for growth regulation in various tissues, including MM. Using array CGH, amplification of 24-OHase was recently detected as a likely target oncogene of the amplification unit 20q13.2 in breast cancer cell lines and tumors. Additionally, amplification of 1alpha-OHase has been reported in human malignant glioma. Using immunohistochemistry, we have now detected nuclear Vitamin D receptor (VDR) immunoreactivity in primary cutaneous malignant melanoma (MM), indicating that Vitamin D metabolites may be of importance for the growth regulation in these tumors. Using Southern analysis, we have analyzed MM and metastases for evidence of amplification of 1alpha-OHase or 24-OHase genes. Our results do not support the hypothesis that amplification of 1alpha-OHase or 24-OHase genes may be of importance for pathogenesis or progression of MM.
Reichrath
Jörg
J
Department of Dermatology, The Saarland University Hospital, Kirrberger Str., 66421 Homburg/Saar, Germany. hajrei@uniklinik-saarland.de
Rafi
Leyla
L
Rech
Martin
M
Meineke
Viktor
V
Tilgen
Wolfgang
W
Seifert
Markus
M
D015139:Blotting, Southern; D002890:Chromosomes, Human, Pair 20; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000201:enzymology / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000378:metabolism; D012878:Skin Neoplasms / Q000201:enzymology / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
D018167:Receptors, Calcitriol; D013250:Steroid Hydroxylases