[Effect of various storage methods on cellular compatibility of bio-derived bone].
OBJECTIVE
To study the effect of various storage methods on cellular compatibility of bio-derived bone.


METHODS
Freeze-dried biomaterials had been stored in two different preservation solutions for three months, while the biomaterials stored for the same time were observed as control group. The experiment was divided into groups A, B, C and D according to different storage methods (group A: with materials stored in preservation solution 1; group B: with materials stored in preservation solution 2; group C: with freeze-dried materials; and group D: simple osteoblasts). Osteoblasts at 2 x 10(6)/ml had been cocultured with materials for 1, 3, 5, and 7 days. The cell-material complex was observed under phase microscope and electronic scanning microscope to evaluate the adhesion and growth of osteoblasts; the cell viability and alkaline phosphatase(ALP) activity were measured, and the cell cycle was analysed by flow cytometer.


RESULTS
Osteoblasts adhered to materials preserved by different methods, differentiated and proliferated in the hole of materials. The difference of cell viability was not significant between three groups on day 1 and day 3. The cell viability of osteoblasts adhered to three materials was A > C > B group on day 5 and day 7 (P < 0.01, P < 0.05). The ALP activity of osteoblasts adhered to three materials was A > C > B group on day 7 (P < 0.01). The cell cycle of different groups did not change significantly, the abnormal cells were not seen.


CONCLUSIONS
The choice of proper preservation solution can optimize the cellular compatibility of bio-derived bone.
Lan
Xu
X
Division of Stem Cell and Tissue Engineering, Key Laboratory Biotherapy of Human Diseases of Ministry of Education West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
Yang
Zhiming
Z
Luo
Jingcong
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018786:Bone Substitutes; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D015925:Cryopreservation; D006648:Histocompatibility; D019188:Organ Preservation Solutions / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D019188:Organ Preservation Solutions; D000469:Alkaline Phosphatase