title: Egg yolk phosvitin inhibits hydroxyl radical formation from the fenton reaction.
abstract: Phosvitin, a phosphoprotein known as an iron-carrier in egg yolk, binds almost all the yolk iron. In this study, we investigated the effect of phosvitin on Fe(II)-catalyzed hydroxyl radical ((.-)OH) formation from H(2)O(2) in the Fenton reaction system. Using electron spin resonance (ESR) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and deoxyribose degradation assays, we observed by both assays that phosvitin more effectively inhibited (.-)OH formation than iron-binding proteins such as ferritin and transferrin. The effectiveness of phosvitin was related to the iron concentration, indicating that phosvitin acts as an antioxidant by chelating iron ions. Phosvitin accelerates Fe(II) autoxidation and thus decreases the availability of Fe(II) for participation in the (.-)OH-generating Fenton reaction. Furthermore, using the plasmid DNA strand breakage assay, phosvitin protected DNA against oxidative damage induced by Fe(II) and H(2)O(2). These results provide insight into the mechanism of protection of the developing embryo against iron-dependent oxidative damage in ovo.
authors.affiliation: School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan. ishikawa@vmas.kitasato-u.ac.jp
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yano
authors.forename: Keizo
authors.initials: K
authors.lastname: Arihara
authors.forename: Makoto
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002642:Chick Embryo; D004249:DNA Damage / Q000187:drug effects; D004527:Egg Proteins / Q000737:chemistry / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D010774:Phosvitin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004527:Egg Proteins; C045076:Fenton's reagent; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; D010774:Phosvitin; D006861:Hydrogen Peroxide; D007501:Iron