Tetraspan and beta-1 integrins expression pattern of the epithelial lung adenocarcinoma cell line A549 and its sensitivity to divalent cations.
BACKGROUND
Tetraspans are ubiquitous integral transmembrane molecules associated on the cell surface with such adhesion molecules as integrins. Their expression has been shown to vary in tumors, but has seldom been described on lung tumoral epithelial cells, and the conditions required for a proper association of tetraspans and integrins have not yet been fully explored.


METHODS
We investigated the expression of 10 tetraspans and six beta1 integrins on the tumoral lung epithelial cell line A549. Cells were examined both in quantitative flow cytometry and as monolayers, under normal or chelated conditions, in order to determine the cation dependency of their expression.


RESULTS
Five tetraspans and four beta1 integrins are expressed on the membrane of A549 cells. Both quantitative and qualitative surface and cytoplasmic modifications of this pattern were induced in chelating conditions, suggesting the importance of divalent cations for the expression of these molecules.


CONCLUSIONS
These data indicate that a specific pattern of tetraspans and integrins, relying strongly on the availability of divalent cations in the microenvironment, is expressed by tumoral epithelial cells.
Massin
Frédéric
F
Laboratoire d'Immunologie, Faculté de Médecine de Nancy, Vandoeuvre lès Nancy, France.
Rubinstein
Eric
E
Faure
Gilbert C
GC
Martinet
Yves
Y
Boucheix
Claude
C
Béné
Marie C
MC
D000230:Adenocarcinoma; D015703:Antigens, CD / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D039641:Integrin beta Chains / Q000378:metabolism*; D008175:Lung Neoplasms; D008565:Membrane Proteins / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism*
D015703:Antigens, CD; D002413:Cations, Divalent; D002614:Chelating Agents; D039641:Integrin beta Chains; D008565:Membrane Proteins; D004492:Edetic Acid