The 3' termini of small RNAs in Trypanosoma brucei.
Post-transcriptional 3' end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3' ends of mature RNAs is essential for defining 3'-processing activities. We have mapped the mature 3' ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear RNAs, the U3 small nucleolar RNA, and the 5S and 5.8S ribosomal RNAs by ligation-mediated PCR in Trypanosoma brucei. With the exception of U5, two classes of 3' ends were observed: flush with the base of a stem-loop structure, and 1-2 nt extended from a stem-loop. Multiple mechanisms and structural features are likely to influence 3' end maturation of RNAs.
Hitchcock
Robert A
RA
Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, 4825 Molecular Sciences Building, 609 Charles E. Young Drive, 90095-1489, USA.
Zeiner
Gusti M
GM
Sturm
Nancy R
NR
Campbell
David A
DA
D000818:Animals; D009690:Nucleic Acid Conformation; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D020537:RNA, Small Nucleolar / Q000737:chemistry / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D016053:RNA, Protozoan; D012340:RNA, Ribosomal, 5.8S; D012341:RNA, Ribosomal, 5S; D012342:RNA, Small Nuclear; D020537:RNA, Small Nucleolar; C117378:RNA, U3 small nucleolar; C405483:U1 small nuclear RNA; C405484:U2 small nuclear RNA; C405482:U4 small nuclear RNA; C405485:U5 small nuclear RNA; C405486:U6 small nuclear RNA