Vitamin E activates CRABP-II gene expression in cultured human fibroblasts, role of protein kinase C.
The treatment of human fibroblasts with different tocopherols in the presence of retinol caused an increase in cytoplasmic retinoic acid binding protein II (CRABP-II) mRNA and protein. The possibility of an involvement of protein kinase C (PKC) in the response to tocopherols was supported by the results obtained with the PKC-specific inhibitors, calphostin C and bisindolylmaleimide I. The effect of alpha-tocopherol was prevented by okadaic acid, suggesting that a protein phosphatase is responsible for PKC dephosphorylation produced by the presence of tocopherols. The results shown support the hypothesis that phosphorylation/dephosphorylation of RXRalpha via PKC may be involved in the regulation of CRABP-II gene expression.
Gimeno
Amparo
A
Departamento de Bioquímica y Biología Molecular, Facultades de Farmacia y Medicina, Avda. Vicent Andrés Estellés s/n, Universidad de Valencia, Valencia, Spain.
Zaragozá
Rosa
R
Viña
Juan R
JR
Miralles
Vicente J
VJ
D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D019319:Okadaic Acid / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology
D017931:DNA Primers; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D014810:Vitamin E; D019319:Okadaic Acid; D011493:Protein Kinase C; D024502:alpha-Tocopherol