The role of IL-18 in the modulation of matrix metalloproteinases and migration of human natural killer (NK) cells.
In this study, we examined whether interleukin-18 (IL-18) affects natural killer (NK) cells' migration and matrix metalloproteinases (MMPs) production. We demonstrated that chemotaxis of human NK cells through basement membrane-like Matrigel was augmented by IL-18. As well, IL-18 stimulation induces the production of activated forms of matrix metalloproteinase-2 (MMP-2) as well as the production of pro-MMP-2 from NK cells. We also demonstrated that MT1-MMP expression on human NK cells, which is a major activator of MMP-2, was induced by IL-18 stimulation coordinated with MMP-2 activation. These data suggest that the MT1-MMP/MMP-2 system participates in the degradation of basement membrane components and thus contributes to NK cell migration.
Ishida
Yukiko
Y
Department of Clinical Pharmacology and Health Research Center, Nagasaki University Japan.
Migita
Kiyoshi
K
Izumi
Yasumori
Y
Nakao
Kazuhiko
K
Ida
Hiroaki
H
Kawakami
Atsushi
A
Abiru
Seigo
S
Ishibashi
Hiromi
H
Eguchi
Katsumi
K
Ishii
Nobuko
N
D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D020382:Interleukin-18 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020382:Interleukin-18; D012333:RNA, Messenger; D011994:Recombinant Proteins; D020778:Matrix Metalloproteinase 2