In vitro studies on the potential for biological control on Aspergillus section Flavi by Kluyveromyces spp.
OBJECTIVE
Antagonist activity of Kluyveromyces spp. isolates on Aspergillus section Flavi was studied.


RESULTS
The screening of isolates were made through studies of growth at different water activities and temperatures, index of dominance (I(D)), ecological similarity, antifungal activity and impact on aflatoxin B1 accumulation. High optical density was obtained at 25 and 30 degrees C and 48 h of incubation. Cell growth decreases with decrease in water activity. The predominant interaction was mutual intermingling at a(w) = 0.982 and 0.955, while at a(w) = 0.999 and 0.937 mutual inhibition for contact was exhibited. All isolates were catabolically identical to Aspergillus section Flavi and compete by nutritional source. At high water activities yeasts showed inhibitory activity on Aspergillus strains, inhibition percentages varied between 75 and 100%. The isolates Y9, Y14, Y16, Y22, Y25 and Y33 showed antifungal activity and inhibitory activity on aflatoxin B1 accumulation at all water activities assayed from all Aspergillus section Flavi strains.


CONCLUSIONS
The data show that the isolates selected in a wide range of environmental conditions could exert their roll like biological control agents for Aspergillus section Flavi in storage maize ecosystem.


CONCLUSIONS
Isolates of Kluyveromyces spp. may have practical value in the postharvest control of storage maize.
La Penna
M
M
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Nesci
A
A
Etcheverry
M
M
D016604:Aflatoxin B1 / Q000032:analysis; D000898:Antibiosis; D001230:Aspergillus / Q000254:growth & development* / Q000378:metabolism; D007716:Kluyveromyces / Q000378:metabolism*; D010572:Pest Control, Biological / Q000379:methods; D013696:Temperature; D014867:Water / Q000737:chemistry
D014867:Water; D016604:Aflatoxin B1