title: Studies on the allostimulatory function of dendritic cells from HCV-HIV-1 co-infected patients.
abstract: There is increasing recognition of the potential morbidity and mortality associated with HIV-1 and hepatitis C (HCV) co-infection. HIV appears to adversely affect HCV disease while the reciprocal effect of HCV on HIV remains controversial. We therefore studied the effect of co-infection on dendritic cell function versus HIV infection alone, as previous work has shown that HCV impairs dendritic cell (DC) function. HIV-1 positive individuals with HCV were matched for CD4 count, HIV-1 RNA viral load and therapy, to HIV-1 positive patients without HCV. Monocyte-derived DC were generated and mixed leukocyte reactions were performed. We assessed allostimulatory capacity with and without administration of exogenous Th1 cytokines, using thymidine uptake and cell division analyses with the vital dye CFSE. We found that monocyte-derived DC from co-infected individuals showed no significant differences in allostimulatory capacity to ex vivo generated DC from HIV-1 infected individuals without HCV. Unlike the situation with HCV infection alone, this impairment was not reversed by increasing concentrations of either interleukin-2 or -12. Monocyte-derived DC from HIV-1 and HCV co-infected individuals have a similar allostimulatory capacity to DC from matched patients with HIV-1 alone. These findings are compatible with results of prior clinical studies that found no evidence that HCV co-infection altered HIV disease progression and has implications for immunotherapeutic approaches in co-infected individuals.
authors.affiliation: The Department of Immunology, Division of Investigative Science, Faculty of Medicine, Imperial College of Science, Technology and Medicine, The Chelsea and Westminster Hospital, 369 Fulham Road, London SW10 9NH,UK. j.stebbing@imperial.ac.uk
authors.forename: Justin
authors.initials: J
authors.lastname: Stebbing
authors.forename: Steve
authors.initials: S
authors.lastname: Patterson
authors.forename: Simon
authors.initials: S
authors.lastname: Portsmouth
authors.forename: Claire
authors.initials: C
authors.lastname: Thomas
authors.forename: Robert
authors.initials: R
authors.lastname: Glassman
authors.forename: Adrian
authors.initials: A
authors.lastname: Wildfire
authors.forename: Frances
authors.initials: F
authors.lastname: Gotch
authors.forename: Mark
authors.initials: M
authors.lastname: Bower
authors.forename: Mark
authors.initials: M
authors.lastname: Nelson
authors.forename: Brian
authors.initials: B
authors.lastname: Gazzard
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D015331:Cohort Studies; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D018450:Disease Progression; D015658:HIV Infections / Q000150:complications / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D006526:Hepatitis C / Q000150:complications / Q000276:immunology / Q000821:virology*; D006801:Humans; D007159:Immunologic Tests; D013601:T-Lymphocytes / Q000276:immunology