title: Two-photon confocal microscopy: a nondestructive method for studying wound healing.
abstract: Two-photon confocal microscopy is a new technology useful in nondestructive analysis of tissue. The pattern generated from laser-excited autofluorescence and second harmonic signals can be analyzed to construct a three-dimensional, microanatomical, structural image. The healing of full-thickness guinea pig skin wounds was studied over a period of 28 days using two-photon confocal microscopy. Three-dimensional data were rendered from two-dimensional images and compared with conventional, en face, histologic sections. Two-photon confocal microscopy images show resolution of muscle, fascia fibers, collagen fibers, inflammatory cells, blood vessels, and hair. Although these images do not currently have the resolution of standard histology, the ability to noninvasively acquire three-dimensional images of skin promises to be an important tool in wound-healing studies.
authors.affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Navarro
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
authors.forename: Rubin
authors.initials: R
authors.lastname: Nirmalan
authors.forename: Nina
authors.initials: N
authors.lastname: Kropf
authors.forename: Farrant
authors.initials: F
authors.lastname: Sakaguchi
authors.forename: Christine S
authors.initials: CS
authors.lastname: Park
authors.forename: Hoon B
authors.initials: HB
authors.lastname: Lee
authors.forename: Dennis P
authors.initials: DP
authors.lastname: Orgill
mesh_terms: D000818:Animals; D005260:Female; D006168:Guinea Pigs; D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000293:injuries / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*