title: Performance of a substratum-irradiated photosynthetic biofilm reactor for the removal of sulfide from wastewater.
abstract: The performance of a sulfide-removal system based on biofilms dominated by green sulfur bacteria (GSB) has been investigated. The system was supplied with radiant energy in the band 720-780 nm, and fed with a synthetic wastewater. The areal net sulfide removal rate and the efficacy of the incident radiant energy for sulfide removal have been characterized over ranges of bulk sulfide concentration (1.6-11.5 mg L(-1)) and incident irradiance (0.21-1.51 W m(-2)). The areal net sulfide removal rate increased monotonically with both increasing incident irradiance and increasing bulk sulfide concentration. The efficacy of the radiant energy for sulfide removal (the amount of sulfide removed per unit energy supplied) also increased monotonically with rising bulk sulfide concentration, but exhibited a maximum value with respect to incident irradiance. The maximum observed values of this net removal rate and this efficacy were, respectively, 2.08 g m(-2) d(-1) and 2.04 g W(-1) d(-1). In-band changes in the spectral composition of the radiant energy affected this efficacy only slightly. The products of sulfide removal were sulfate and elemental-S. The elemental-S was scarcely released into the liquid, however, and reasons for this, such as sulfur reduction and polysulfide formation, are considered. Between 1.45 and 3.85 photons were needed for the net removal of one electron from S-species. Intact samples of the biofilm were characterized by microscopy, and their thicknesses lay between 39 +/- 9 and 429 +/- 57 microm. The use of the experimentally determined rates and efficacies for the design of a pilot-scale system is illustrated.
authors.affiliation: Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Qld 4072, Australia. timhurse@yahoo.com.au
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Hurse
authors.forename: Jurg
authors.initials: J
authors.lastname: Keller
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development* / Q000528:radiation effects; D019149:Bioreactors / Q000382:microbiology*; D019414:Chlorobi / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008027:Light; D013440:Sulfides; D013455:Sulfur; D014874:Water Pollutants, Chemical; D018508:Water Purification / Q000379:methods*
chemical_list: D013440:Sulfides; D014874:Water Pollutants, Chemical; D013455:Sulfur