title: Analysis of murine leukemia virus replication complemented by yeast tRNA(Phe) reveals inherent preferences for the tRNA primer selected for reverse transcription.
abstract: The replication of murine leukemia virus (MuLV) requires the capture of a cellular tRNA(Pro) as a primer for reverse transcription. To further study the specificity of primer selection, we have utilized a defective MuLV in which the primer-binding site (PBS) has been altered to be complementary to a nonmammalian tRNA, yeast tRNA(Phe). Infectivity of the defective MuLV is dependent upon co-expression of yeast tRNA(Phe) in the cell. Defective MuLV genomes have been constructed in which the PBS was altered to be complementary to tRNA(Phe) that also encoded the cDNA for tRNA(Phe). Transfection of these defective proviral genomes into cells resulted in the production of infectious MuLV as determined by a single-round assay. The amount of infectious virus produced using this complementation system, though, was approximately 6-fold lower than that produced following transfection of defective proviral genomes with a wild-type PBS complementary to tRNA(Pro). The lower infectivity was not due to reduced expression of tRNA(Phe) in the transfected cells as compared to endogenous tRNA(Pro) or tRNA(Lys,3). Serial passage of the MuLV genome with a PBS complementary to tRNA(Phe) that encoded tRNA(Phe) resulted in amplification of the virus. Using this rescue system, we have passaged the virus for four serial passages, after which time a revertant genome in which the PBS was altered to be complementary to tRNA(Gln) was detected that grew to high titers following subsequent serial passage. The results of these studies suggest that MuLV has preferences for the tRNA primer used in reverse transcription and are discussed with respect to the mechanism of primer selection.
authors.affiliation: Department of Cell Biology, University of Alabama at Birmingham, 720 20th Street South, Birmingham, AL 35294, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Palmer
authors.forename: Casey D
authors.initials: CD
authors.lastname: Morrow
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe / Q000235:genetics* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D016865:Reassortant Viruses; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C034477:RNA primers; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe; D012313:RNA; D012194:RNA-Directed DNA Polymerase