Rapid and sensitive NMR method for osmolyte determination.
We propose a rapid and sensitive method for osmolyte determination, based on one-dimensional and two-dimensional 1H NMR spectroscopy applied directly on culture of haloalkalophilic Halomonas pantelleriensis and acidothermophilic archaeon Sulfolobus solfataricus, without any extraction procedure. The osmoprotectants hydroxyectoine, ectoine, glutamate, glycine-betaine and treahalose can easily be quantified by integrating the peak areas with respect to an internal standard, and the concentrations evaluated with this method are in excellent agreement with the values previously reported. Furthermore, trace amount of osmoprotectants, often undetectable after extraction procedures, can also be evaluated.
Motta
Andrea
A
Istituto di Chimica Biomolecolare del CNR, Comprensorio Olivetti, Edificio 70, via Campi, Flegrei 34, Pozzuoli I-80078, Italy. andrea.motta@icb.cnr.it
Romano
Ida
I
Gambacorta
Agata
A
D000599:Amino Acids, Diamino / Q000032:analysis*; D001622:Betaine / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis; D020632:Halomonas / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000706:statistics & numerical data; D008828:Microbiological Techniques / Q000706:statistics & numerical data; D009995:Osmosis; D012680:Sensitivity and Specificity; D016944:Sulfolobus / Q000737:chemistry; D014199:Trehalose / Q000032:analysis
D000599:Amino Acids, Diamino; C121557:hydroxyectoine; D018698:Glutamic Acid; D001622:Betaine; C045628:ectoine; D006859:Hydrogen; D014199:Trehalose