title: Vitamin E activates CRABP-II gene expression in cultured human fibroblasts, role of protein kinase C.
abstract: The treatment of human fibroblasts with different tocopherols in the presence of retinol caused an increase in cytoplasmic retinoic acid binding protein II (CRABP-II) mRNA and protein. The possibility of an involvement of protein kinase C (PKC) in the response to tocopherols was supported by the results obtained with the PKC-specific inhibitors, calphostin C and bisindolylmaleimide I. The effect of alpha-tocopherol was prevented by okadaic acid, suggesting that a protein phosphatase is responsible for PKC dephosphorylation produced by the presence of tocopherols. The results shown support the hypothesis that phosphorylation/dephosphorylation of RXRalpha via PKC may be involved in the regulation of CRABP-II gene expression.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultades de Farmacia y Medicina, Avda. Vicent Andrés Estellés s/n, Universidad de Valencia, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Gimeno
authors.forename: Rosa
authors.initials: R
authors.lastname: Zaragozá
authors.forename: Juan R
authors.initials: JR
authors.lastname: Viña
authors.forename: Vicente J
authors.initials: VJ
authors.lastname: Miralles
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D019319:Okadaic Acid / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D014810:Vitamin E; D019319:Okadaic Acid; D011493:Protein Kinase C; D024502:alpha-Tocopherol