Effects of chlordane and lindane on testosterone and vitellogenin levels in green neon shrimp (Neocaridina denticulata).
The purpose of this study was to investigate the acute toxicity of chlordane and lindane as well as their endocrine disruption effect on green neon shrimp (Neocaridina denticulata), a common habitant in freshwater system of eastern Asia and Hawaii. First, the organisms were exposed to chlordane and lindane to estimate the 96-h LC(50)(96-h median lethal concentration). Then, levels of testosterone and vitellogenin in hemolymph of N. denticulata after exposure to sublethal concentrations of chlordane (1 ng/L and 10 ng/L) and lindane (0.1 microg/L and 1 microg/L) were also examined. The 96-h LC(50) values obtained from the results of acute exposure were 127.03 (130.11-122.35) ng/L and 9.36 (8.00-10.96) microg/L for chlordane and lindane, respectively. Furthermore, reductions of testosterone concentration were observed in both chlordane- and lindane-treated shrimps, whereas induction of vitellogenin-like protein was only apparent in chlordane-treated shrimps. Thus, it is concluded that chlordane and lindane may probably show some disruption endocrine functions on N. denticulata.
Huang
Da-Ji
DJ
Institute of Zoology, National Taiwan University, No. 1 Sec. 4 Roosevelt Road, Taipei 106, Taiwan, Republic of China.
Chen
Hon-Cheng
HC
D000818:Animals; D002706:Chlordan / Q000008:administration & dosage / Q000633:toxicity*; D033364:Decapoda / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D001556:Hexachlorocyclohexane / Q000008:administration & dosage / Q000633:toxicity*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D013739:Testosterone / Q000378:metabolism*; D013997:Time Factors; D014819:Vitellogenins / Q000378:metabolism*
D007306:Insecticides; D014819:Vitellogenins; D002706:Chlordan; D013739:Testosterone; D004958:Estradiol; D001556:Hexachlorocyclohexane