[Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen].
OBJECTIVE
To observe immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen (HBcAg).


METHODS
A new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen was constructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice were immunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linked immune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH release assay.


RESULTS
pSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showed strong anti-HBc response and specific high cytotoxicity of CTL.


CONCLUSIONS
pSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice.
Xing
Yi-ping
YP
Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Huang
Zu-hu
ZH
Wang
Shi-xia
SX
Cai
Jie
J
Li
Jun
J
Lu
Shan
S
D000818:Animals; D006510:Hepatitis B Antibodies / Q000097:blood*; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA