title: Chronic orchidectomy does not influence the sensitivity of the pituitary somatotropes to varying doses of GHRH administered intravenously to the adult male rhesus monkey.
abstract: In the present study, the pituitary growth hormone (GH) response to graded doses of GH-releasing hormone (GHRH) was determined in intact (n = 3) and chronically orchidectomized (n = 3) adult rhesus monkeys (Mucaca mulatta). GHRH in doses of 0, 6.25, 12.5 and 25 microg/kg BW was infused through a teflon cannula implanted in the saphenous vein. Blood samples were collected 60 min before and 90 min after the injection of the neurohormone at 15 min intervals. All bleedings were carried out under ketamine hydrochloride anesthesia. The plasma levels of GH were determined by using AutoDELFIA time-resolved flouroimmunoassay, whereas plasma levels of testosterone and estradiol were determined using specific radioimmunoassay systems. The GH responses to GHRH were not significantly different between intact and chronically orchidectomized monkeys at any of the dose levels tested (p > 0.05). The administration of GHRH resulted in a significant (p < 0.05) stimulation of GH secretion at all the doses tested and in both the groups studied. In both intact and orchidectomized animals, the greatest response was observed at 6.25 microg/kg and no further increase was noted with the higher doses of GHRH. In conclusion, the present study suggests that chronic orchidectomy does not influence the sensitivity of the pituitary somatotropes to GHRH stimulation implying that the responsiveness of the pituitary somatotropes to GHRH is independent of testicular steroid modulation.
authors.affiliation: Pakistan Science Foundation, Constitution Avenue, G-5/2, Islamabad, Pakistan. shakeel_raza@hotmail.com
authors.forename: S S R
authors.initials: SS
authors.lastname: Rizvi
authors.forename: S
authors.initials: S
authors.lastname: Altaf
authors.forename: S
authors.initials: S
authors.lastname: Latif
authors.forename: A A
authors.initials: AA
authors.lastname: Naseem
authors.forename: M
authors.initials: M
authors.lastname: Afzal
authors.forename: M
authors.initials: M
authors.lastname: Qayyum
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood; D015200:Fluoroimmunoassay; D013006:Growth Hormone / Q000097:blood* / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D008253:Macaca mulatta / Q000378:metabolism*; D008297:Male; D009919:Orchiectomy / Q000662:veterinary*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D011863:Radioimmunoassay; D013739:Testosterone / Q000097:blood; D013997:Time Factors
chemical_list: D013739:Testosterone; D004958:Estradiol; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone