title: [Histologic changes in the jejunal wall after autotransplantation in a dog].
abstract: BACKGROUND
  Damages to the small intestinal wall resulting from ischemic-reperfusion changes, represent common complications of the clinical transplantation of the small intestine.
  
  
  OBJECTIVE
  Evaluation and quantification of the histological changes in the jejunal wall following its autotransplantation in a dog using the scale according to Park.
  
  
  METHODS
  In dogs (n = 8), mongrel of both sexes, aged from 6 months to 2 years, weighting from 10 to 25 kgs, a resection of the jejunum followed by its autotransplantation was performed. At the time of the jejunal harvest, then after one-hour-long cold ischemia, 20 minutes after its reperfusion and then the 10th and the 20th day after the transplantation, bioptic samples of the whole jejunal wall were taken to be examined histologically. After being stained with hematoxyllin-eosine, the samples were evaluated according to the Park grading system.
  
  
  METHODS
  The severity of the jejunal wall damage at the respective biopsy samples collection times was evaluated using the t-test for two dependent samples.
  
  
  RESULTS
  After an hour-long cold ischemia, signs of increasing damage to the intestinal wall were observed, when compared to the peroperative sample (0 +/- 0) up to the degree 0.68 +/- 0.5 of the Park grading schema (p < 0.05). This damage degree increased 20 minutes after the reperfusion up to the value of 4 +/- 0 (p < 0.05). On the 10th and the 20th day a practically normal histological picture of the jejunal wall was observed. The histological changes in both cases were graded 0.38 +/- 0.5 (p < 0.05).
  
  
  CONCLUSIONS
  Maximum histological changes following the autotransplantation of the small intestine with an hour-long cold ischemia were observed 20 minutes after the reperfusion. After 10 postoperative days, a practically normal histological picture of the small intestinal wall structure was observed. It remained unchanged even on the 20th postoperative day.
authors.affiliation: Klinika transplantacní chirurgie, Institut klinické a experimentální medicíny, Praha. peba@medicon.cz
authors.forename: P
authors.initials: P
authors.lastname: Baláz
authors.forename: I
authors.initials: I
authors.lastname: Matia
authors.forename: J
authors.initials: J
authors.lastname: Kiss
authors.forename: S
authors.initials: S
authors.lastname: Jackanin
authors.forename: S
authors.initials: S
authors.lastname: Rokosný
authors.forename: P
authors.initials: P
authors.lastname: Wohlfahrt
authors.forename: S
authors.initials: S
authors.lastname: Pingorová
authors.forename: M
authors.initials: M
authors.lastname: Pomfy
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D007583:Jejunum / Q000473:pathology / Q000637:transplantation*; D008297:Male; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology*; D014182:Transplantation, Autologous