title: An IAP-IAP complex inhibits apoptosis.
abstract: Regulators of apoptosis are thought to work in concert, but the molecular interactions of this process are not understood. Here, we show that in response to cell death stimulation, survivin, a member of the inhibitor of apoptosis (IAP) gene family, associates with another IAP protein, XIAP, via conserved baculovirus IAP repeats. Formation of a survivin-XIAP complex promotes increased XIAP stability against ubiquitination/proteasomal destruction and synergistic inhibition of apoptosis, which is abolished in XIAP(-/-) cells. Therefore, orchestration of an IAP-IAP complex regulates apoptosis.
authors.affiliation: Department of Cancer Biology and the Cancer Center, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Dohi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Okada
authors.forename: Fang
authors.initials: F
authors.lastname: Xia
authors.forename: Casey E
authors.initials: CE
authors.lastname: Wilford
authors.forename: Temesgen
authors.initials: T
authors.lastname: Samuel
authors.forename: Kate
authors.initials: K
authors.lastname: Welsh
authors.forename: Hiroyouki
authors.initials: H
authors.lastname: Marusawa
authors.forename: Hua
authors.initials: H
authors.lastname: Zou
authors.forename: Robert
authors.initials: R
authors.lastname: Armstrong
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Matsuzawa
authors.forename: Guy S
authors.initials: GS
authors.lastname: Salvesen
authors.forename: John C
authors.initials: JC
authors.lastname: Reed
authors.forename: Dario C
authors.initials: DC
authors.lastname: Altieri
mesh_terms: D000818:Animals; D017209:Apoptosis; D016367:Baculoviridae / Q000378:metabolism; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D002460:Cell Line; D045744:Cell Line, Tumor; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D051034:Inhibitor of Apoptosis Proteins; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009363:Neoplasm Proteins; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D000077022:Survivin; D013997:Time Factors; D014162:Transfection; D019076:Transgenes; D025801:Ubiquitin / Q000378:metabolism; D051636:X-Linked Inhibitor of Apoptosis Protein
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009097:Multienzyme Complexes; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins; D000077022:Survivin; D025801:Ubiquitin; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D005982:Glutathione Transferase; C506433:CASP9 protein, human; C506434:Casp9 protein, mouse; D053453:Caspase 9; D020169:Caspases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex