title: Bionanotechnology based on silica nanoparticles.
abstract: We have developed uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water-in-oil microemulsion method. The nanoparticles are highly luminescent and photostable with the size ranging from 5 nm to 400 nm. Bioconjugation of these silica nanoparticles adds unique biofunctions with various molecules such as enzymes, antibodies, and DNA molecules. Significant advantages have been shown in using bioconjugated nanoparticles for biosensing and bioimaging, such as cell staining, DNA detection and separation, rapid single bacterium detection, and biotechnological application in DNA protection.
authors.affiliation: Center for Research at the Bio/nano Interface, Department of Chemistry, University of Florida, Gainesville 32611, USA. tan@chem.ufl.edu
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.forename: Xiaojun Julia
authors.initials: XJ
authors.lastname: Zhao
authors.forename: Timothy
authors.initials: T
authors.lastname: Drake
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Rahul P
authors.initials: RP
authors.lastname: Bagwe
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D015415:Biomarkers / Q000737:chemistry; D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry; D008280:Magnetics; D008967:Molecular Biology / Q000379:methods*; D036103:Nanotechnology / Q000379:methods* / Q000639:trends; D010316:Particle Size; D038761:Photobleaching; D012822:Silicon Dioxide; D013499:Surface Properties
chemical_list: D015415:Biomarkers; D004396:Coloring Agents; D012822:Silicon Dioxide; D004247:DNA