Protein microarrays in diagnosing IgE-mediated diseases: spotting allergy at the molecular level.
Over the last few decades, the prevalence of allergic diseases has increased dramatically in developed nations. The resulting worldwide burden on healthcare systems has provoked a whole series of research initiatives among allergy experts and commercial companies that aim to develop novel tests to improve the diagnostic risk assessment and early preventive treatment of disease. The advent of protein microarray technology has fueled aspirations of multianalyte immunological applications that permit the simultaneous analysis of huge numbers of disease-related parameters that will hopefully become amenable in the near future. Allergen microarrays have been developed for the monitoring of patient-specific antibody profiles to a previously unknown variety of allergens in a single analytical step. This review describes significant discoveries and developments in allergy research against a background of the increasing prevalence of disease and hence the emerging challenges for national healthcare systems. The development of novel protein microarray-based allergy diagnostic tests is portrayed in concert with the recent advances and benefits of this technology, along with the challenges that must be met by manufacturers in order to succeed with innovative allergen microarrays in a highly competitive market.
Harwanegg
Christian
C
VBC - Genomics Bioscience Research, Rennweg 95 B, 1030 Vienna, Austria. christian.harwanegg@vbc-genomics.com
Hiller
Reinhard
R
D000485:Allergens / Q000032:analysis; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000628:therapy; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000628:therapy; D007073:Immunoglobulin E / Q000276:immunology*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000191:economics / Q000592:standards
D000485:Allergens; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E