[Study on using the hen's egg test-chorioallantoic membrane as an alternative method of draize eye irritation test].
OBJECTIVE
To establish and study the hen's egg test-chorioallantoic membrane (HET-CAM) as an alternative method of Draize eye irritation test (Draize test).


METHODS
14 cosmetic ingredients were tested by the Hen's egg test-chorioallantoic membrane score (HET-CAM score) and the chorioallantoic membrane-trypan blue staining (CAM-TB) methods.


RESULTS
Showed that compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and score of 24 h after application (S24), the correlation coefficient between HET-CAM score and MAS or S24 was 0.847 or 0.779, while that between CAB-TB and MAS or S24 was 0.862 or 0.831 respectively. The results also showed that CAM-TB had a higher correlation with Draize test than HET-CAM score, partly because it is objective and quantitative. Also, the results showed that HET-CAM score had the greatest correlation with conjunctivae score of the three components, and so did the CAM-TB with corneal score.


CONCLUSIONS
It is suggested that the two types of HET-CAM can be used in a combined manner as an effective alternative method to Draize test.
Liao
Yan
Y
West China School of Public Health, Sichuan University, Chengdu 610041, China.
Wang
Xue
X
Zhang
Lishi
L
Li
Guanmin
G
D000482:Allantois / Q000166:cytology / Q000187:drug effects*; D000826:Animal Testing Alternatives; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000166:cytology / Q000187:drug effects*; D003228:Conjunctiva / Q000187:drug effects; D003358:Cosmetics / Q000633:toxicity*; D006801:Humans; D007509:Irritants / Q000633:toxicity; D011358:Product Surveillance, Postmarketing; D011786:Quality Control; D012680:Sensitivity and Specificity; D018675:Toxicity Tests
D003358:Cosmetics; D007509:Irritants