Effect of curcumin on gelatinase A (MMP-2) activity in B16F10 melanoma cells.
Treatment of highly metastatic murine melanoma cells B16F10 with curcumin (15 microM) for 15 days significantly inhibited matrixmetalloproteinase-2 (MMP-2) activity. Expression of membrane type-1 matrix metalloproteinase (MT1-MMP) and focal adhesion kinase (FAK), an important component of the intracellular signalling pathway, were also reduced to almost background levels. MMP-2, MT1-MMP and FAK did not return to control levels even after 28 days of drug withdrawal. However, effect of curcumin on ligand binding property of integrin receptors was reversible. Downregulation of FAK (which would impair integrin mediated signal transduction cascade) and reduction of MMP-2 activity could be important reasons for anti-metastatic property of curcumin.
Banerji
Aniruddha
A
Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700-026, India.
Chakrabarti
Jayati
J
Mitra
Aparna
A
Chatterjee
Amitava
A
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D016023:Integrins / Q000502:physiology; D020778:Matrix Metalloproteinase 2 / Q000494:pharmacology*; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis / Q000503:physiopathology; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*
D000970:Antineoplastic Agents; D016023:Integrins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496051:Ptk2 protein, mouse; D020778:Matrix Metalloproteinase 2; D003474:Curcumin