Fast high-performance liquid chromatography method for quality control of soy extracts.
Soy extracts contain a mixture of isoflavones belonging to the group of phytoestrogens. In the quality control of soy the amount of isoflavones, both aglycones and glycosides, is usually determined by means of reversed-phase HPLC-UV. On conventional C18-material columns, long analysis times are required in order to separate this complex mixture. In order to speed up analysis, the separation was optimized using two linked monolithic silica-based reversed-phase C18 columns. A spectacular decrease of the analysis time, i.e. almost three-fold, was achieved by applying a flow rate of 3-4 ml/min without loosing any separation efficiency. This analysis method for determination of isoflavones in soy extracts in less than 25 min was fully validated according to the ICH guidelines.
Apers
Sandra
S
Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium. sandra.apers@ua.ac.be
Naessens
Tania
T
Van Den Steen
Katleen
K
Cuyckens
Filip
F
Claeys
Magda
M
Pieters
Luc
L
Vlietinck
Arnold
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017408:Guidelines as Topic; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D013025:Glycine max / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts