Selectively enriched mRNAs in rat synaptoneurosomes.
Differential display was used to identify synapse-enriched mRNAs. Of 15 mRNAs initially identified, all were found in multiple synaptoneurosome preparations; 58% were subsequently shown to be enriched in all the preparations by Northern blotting and semiquantitative RT-PCR. RNAs involved in signal transduction, vesicle trafficking, lipid modification and cell shape and remodeling were among these messages. Tip60a mRNA, recently found to associate with the fragile X mental retardation protein, was also identified. These data demonstrate the diversity of the local message pool at synapses.
Sung
Y-J
YJ
Department of Anatomy and Cell Biology Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Weiler
I J
IJ
Greenough
W T
WT
Denman
R B
RB
D000818:Animals; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D013569:Synapses / Q000502:physiology; D013574:Synaptosomes / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D012333:RNA, Messenger