[Reversal effect of tetrandrine in combination with droloxifene on cell line K562/A02 and its correlation with inducted apoptosis].
To study the correlation of the reversal effect of tetrandrine (Tet) in combination with droloxifene (DRL) on multidrug resistant cell line K562/A02 and induction apoptosis, the apoptosis of K562 cells and K562/A02 cells after the treatment of Tet and DRL alone or their combination was detected by flow cytometry (FCM). The results showed 0.62 microg/ml Tet and 1.94 microg/ml DRL alone could induce apoptosis of K562 cells in time-dependent manner after culture for 48 hours. Neither Tet at 0.62 microg/ml nor DRL at 1.94 microg/ml alone could induce apoptosis of K562/A02 cells, but in the combination of them two induced apoptosis of few K562/A02 cells in culture for 72 hours. In conclusion, the mechanism of Tet and DRL reversing multidrug resistance has no correlation with the apoptosis of K562/A02 cells.
Zhou
Yun
Y
Department of Hematology, Zhongda Hospital, Medical College of Southeast University, Nanjing 210009, China.
Chen
Bao-An
BA
Dong
Ying
Y
Zhang
Peng
P
Cheng
Jian
J
Sheng
Ming
M
Wang
Ting
T
Gao
Feng
F
D000470:Alkaloids / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects*; D044182:Benzylisoquinolines / Q000008:administration & dosage*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D013629:Tamoxifen / Q000008:administration & dosage* / Q000031:analogs & derivatives*
D000470:Alkaloids; D044182:Benzylisoquinolines; D013629:Tamoxifen; C038345:droloxifene; C009438:tetrandrine