title: Characterization of rtSH3p13 gene encoding a development protein involved in vesicular traffic in spermiogenesis.
abstract: A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qiao
authors.forename: Ju Xiang
authors.initials: JX
authors.lastname: Yang
authors.forename: Xiao Dong L
authors.initials: XD
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian Chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: Shu Dong
authors.initials: SD
authors.lastname: Zong
authors.forename: Shi Ying
authors.initials: SY
authors.lastname: Miao
authors.forename: Lin Fang
authors.initials: LF
authors.lastname: Wang
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D016466:CHO Cells / Q000378:metabolism; D002966:Clathrin / Q000737:chemistry / Q000502:physiology; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000502:physiology; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000737:chemistry / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002966:Clathrin; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D011506:Proteins; C495380:Sh3gl3 protein, rat; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases