title: Serotypes of clinical cerebrospinal fluid Cryptococcus neoformans isolates from southern Taiwan and their in vitro susceptibilities to amphotericin B, fluconazole, and voriconazole.
abstract: In this study, 34 clinical cerebrospinal fluid isolates of Cryptococcus neoformans were serotyped, and their in vitro susceptibilities to amphotericin B, fluconazole, and voriconazole were analyzed. Of these 34 isolates, serotype A was found in 29 isolates and serotype B in the other five. The voriconazole geometric mean MIC was significantly lower than the amphotericin B/antibiotic medium 3 geometric mean MIC (P < 0.0001 at both 48 and 72 h), as well as the fluconazole geometric mean MIC (P < 0.0001 at both 48 and 72 h). Of the three antifungal agents, only fluconazole, with geometric mean MICs at both 48 and 72 h, showed significant difference between the serotypes A and B of C. neoformans.
authors.affiliation: Department of Neurology, Chang Gung Memorial Hospital-Kaohsiung, Kaohsiung Hsien, Taiwan. cwenneng@ms19.hinet.net
authors.forename: Wen-Neng
authors.initials: WN
authors.lastname: Chang
authors.forename: Chi-Ren
authors.initials: CR
authors.lastname: Huang
authors.forename: Chien-Bang
authors.initials: CB
authors.lastname: Lei
authors.forename: Ping-Yu
authors.initials: PY
authors.lastname: Lee
authors.forename: Chun-Chih
authors.initials: CC
authors.lastname: Chien
authors.forename: Hsueh-Wen
authors.initials: HW
authors.lastname: Chang
authors.forename: Chen-Sheng
authors.initials: CS
authors.lastname: Chang
authors.forename: Cheng-Hsien
authors.initials: CH
authors.lastname: Lu
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology / Q000627:therapeutic use; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D015169:Colony Count, Microbial; D003453:Cryptococcosis / Q000188:drug therapy*; D003455:Cryptococcus neoformans / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008826:Microbial Sensitivity Tests; D011743:Pyrimidines / Q000494:pharmacology / Q000627:therapeutic use; D012703:Serotyping; D013624:Taiwan; D014230:Triazoles / Q000494:pharmacology / Q000627:therapeutic use; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole