Genome-wide search for loss of heterozygosity in Chinese patients with sporadic colorectal cancer.
In an attempt to integrally investigate the loss of tumor suppressor genes and search for putative suppressor loci associated with tumor occurrence and progression, we conducted a genome-wide loss of hetero zygosity (LOH) study of 83 tumor samples obtained from Chinese patients with sporadic colorectal cancer. We employed 400 fluorescence-labeled microsatellite marker primers to amplify the corresponding loci of the genomic DNA and then electrophoresed the polymerase chain reaction products and analyzed the fluorescent signals. The LOH frequencies were high (>35%) but were not associated with the tumor stage and progression in 20 loci, including the regions where TP53, E-cadherin, deleted in colorectal carcinoma (DCC), phosphatase and tensin homolog deleted on chromosome 10 (PTEN), mothers against decapentaplegic, Drosophila, homolog of 2 (MADH2) and mothers against decapentaplegic, Drosophila, homolog of 4 (MADH4) reside. Loss of other loci, including two narrow regions on chromosome 2, was found to relate to the tumor stage, suggesting that this genomic instability may contribute to tumor progression.
Peng
Zhihai
Z
Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, The People's Republic of China. pengpzhb@online.sh.cn
Zhang
Fang
F
Zhou
Chongzhi
C
Ling
Yun
Y
Bai
Shaochun
S
Liu
Wanqing
W
Qiu
Guoqiang
G
He
Lin
L
Wang
Liwei
L
Wei
Daoyan
D
Lin
Edward
E
Xie
Keping
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000208:ethnology; D015179:Colorectal Neoplasms / Q000208:ethnology / Q000235:genetics*; D017931:DNA Primers; D018450:Disease Progression; D005260:Female; D016147:Genes, Tumor Suppressor; D016678:Genome; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction
D017931:DNA Primers