[Inhibitory effect of siRNA on bcr-abl gene expression in K562 cell line].
To explore a new way to treat CML, inhibitory effect of small interfering RNA (SiRNA) on bcr-abl fusion gene expression of K562 cell line was studied. SiRNA for bcr-abl gene was designed and transfected into K562 cells, bcr-abl gene expression was tested by RT-PCR. The results showed that bcr-abl gene expression was inhibited by using siRNA in dose-dependent manner and reduced to 19.9% and 26.6% of the control at 24 and 48 hours after transfection with 0.2 micro g siRNA respectively. K562 cells proliferation was suppressed finally, but bcr-abl gene expression restored at 72 hours. In conclusion, anti-bcr-abl siRNA can effectively inhibit bcr-abl gene expression of K562 cell line.
Jiang
Lei
L
Institute of Medical Sciences, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. jianglei0299@hotmail.com
Wu
Jian-Bo
JB
Yu
Kang
K
Ni
Wu-Hua
WH
D004305:Dose-Response Relationship, Drug; D016313:Genes, abl; D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering