Direct detection of renal function markers using microchip CE with pulsed electrochemical detection.
Creatinine, creatine, and uric acid are three important compounds that are measured in a variety of clinical assays, most notably for renal function. Traditional clinical assays for these compounds have focused on the use of enzymes or chemical reactions. Electrophoretic microchips have the potential to integrate separation power of capillary electrophoresis with devices that are small, portable, and have the speed of conventional sensors. The development of a microchip CE system for the direct detection of creatinine, creatine, and uric acid is presented. The device uses pulsed amperometric detection (PAD) to detect the nitrogen-containing compounds as well as the easily oxidizable uric acid. Baseline separation of creatinine, creatine and uric acid was achieved using 30 mM borate buffer (pH = 9.4) in less than 200 s. Linear calibration curves were obtained with limits of detection of 80 microM, 250 microM and 270 microM for creatinine, creatine and uric acid respectively. An optimization of the separation conditions and a comparison of PAD with other amperometric detection modes is also shown. Finally, analysis of a real urine sample is presented with validation of creatinine concentrations using a clinical assay kit based on the Jaffé reaction.
Garcia
Carlos D
CD
Chemistry Department, Colorado State University, Fort Collins, CO 80523, USA.
Henry
Charles S
CS
D015415:Biomarkers / Q000652:urine; D003401:Creatine / Q000652:urine; D003404:Creatinine / Q000652:urine; D004563:Electrochemistry; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D007676:Kidney Failure, Chronic / Q000503:physiopathology*; D008832:Microchemistry; D014527:Uric Acid / Q000652:urine
D015415:Biomarkers; D014527:Uric Acid; D003404:Creatinine; D003401:Creatine