title: [Methemoglobin level and its comparison with levels of globins and membrane proteins in erythrocytes of patients with beta-thalassemia].
abstract: On the basis of conducted biological-and-chemical research, the clinical-and-genetic forms of beta-thalassemia were revealed, as well as fractions and the quantity of globin chains and membrane proteins were defined. The methods of electrophoresis and of isoelectrofocusing were made use of within the case study to separate between the globin chains and the membrane proteins. Samples of venous blood obtained from one hundred and one children with beta-thalassemia and from 45 donors were tested. Higher and lower values for different globin chains were found in investigating the quantitative ratios of various globin chains in patients with beta+, beta 0- and heterozygote variations of beta-thalassemia. Reliable changes in the quantitative content of main membrane proteins were observed in patients with heterozygote beta-thalassemia. The final study data denote that an excessive accumulation of alpha-globin chains in heterozygote beta-thalassemia is accompanied by a higher level of methemoglobin and by changed quantities of globin chains and membrane proteins.
authors.forename: V I
authors.initials: VI
authors.lastname: Iagubova
authors.forename: G A
authors.initials: GA
authors.lastname: Alekperova
authors.forename: T A
authors.initials: TA
authors.lastname: Askerova
authors.forename: T S
authors.initials: TS
authors.lastname: Dadashova
mesh_terms: D002648:Child; D004586:Electrophoresis / Q000379:methods; D004912:Erythrocytes / Q000737:chemistry*; D005319:Fetal Hemoglobin / Q000032:analysis; D005914:Globins / Q000032:analysis*; D006579:Heterozygote; D006801:Humans; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000097:blood*; D008706:Methemoglobin / Q000032:analysis*; D012016:Reference Values; D017086:beta-Thalassemia / Q000097:blood* / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D005914:Globins; D008706:Methemoglobin; D005319:Fetal Hemoglobin