Immunohistochemical localization of type IV collagen alpha chains in the basement membrane of the pancreatic duct in human normal pancreas and pancreatic diseases.
The type IV collagen (Col-IV) consists of 3 alpha chains. Six different alpha chains [alpha1(IV), alpha2(IV), alpha3(IV), alpha4(IV), alpha5(IV), and alpha6(IV)] have been identified, and their combination is considered to be organ specific. We investigated the immunohistochemical localization of alpha (IV) chains in the basement membrane (BM) of the pancreatic duct in human normal pancreas (NP) and pancreatic diseases. Fifty specimens [10 NP, 10 chronic pancreatitis (CP), 10 intraductal papillary mucinous tumor (IPMT), and 20 pancreatic adenocarcinoma (PAC)] were examined. Alpha 1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) were linearly immunostained in NP, CP, and IPMT. In PAC, alpha(IV) and alpha2(IV) were immunostained, but alpha5(IV) and alpha6(IV) were not stained in 30% and 40% of the cases, respectively. In conclusion, immunohistochemically, the Col-IV of human normal pancreatic duct consisted of alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV). alpha5(IV) and alpha6(IV) were frequently absent in PAC, and their absence might be related to the invasion of cancer cells.
Kadono
Genichiro
G
Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Ishihara
Takeshi
T
Yamaguchi
Taketo
T
Kato
Kazuki
K
Kondo
Fukuo
F
Naito
Ichiro
I
Sado
Yoshikazu
Y
Saisho
Hiromitsu
H
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D000236:Adenoma / Q000737:chemistry / Q000648:ultrastructure; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D001485:Basement Membrane / Q000737:chemistry* / Q000648:ultrastructure; D024141:Collagen Type IV / Q000032:analysis* / Q000737:chemistry / Q000502:physiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis; D009928:Organ Specificity; D010182:Pancreatic Diseases / Q000378:metabolism* / Q000473:pathology; D010183:Pancreatic Ducts / Q000737:chemistry* / Q000648:ultrastructure; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology; D021122:Protein Subunits
D000911:Antibodies, Monoclonal; D024141:Collagen Type IV; D009363:Neoplasm Proteins; D021122:Protein Subunits