title: Cytotoxic effect and electrophysiological study on human MRC-5 fibroblasts of R-irniine, a natural alkylpyrrolidine alkaloid.
abstract: R-irniine is a natural 2-alkylpyrrolidine alkaloid extracted from Arisarum vulgare Targ., a species belonging to the Araceae family known in Morocco for its toxicity. Cytotoxic and electrophysiological studies of this compound on human MRC-5 fibroblasts were carried out. Cytotoxic potential of R-irniine was detected on MRC-5 fibroblasts at 40 microg/mL. We carried out the electrophysiological study of this compound on MRC-5 cells by using the technique of patch-clamp in "Whole cell" configuration. R-irniine showed no effect on the outward potassium current of the MRC-5 fibroblasts.
authors.affiliation: Laboratory of Phytochemistry and Pharmacognosy, University Mohamed Ier, Route Sidi Maâfa, B.P. 524, 60000, Oujda, Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Lamkadem
authors.forename: Mohamed
authors.initials: M
authors.lastname: Aziz
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwob
authors.forename: Jacques
authors.initials: J
authors.lastname: Rabier
authors.forename: Jamal
authors.initials: J
authors.lastname: Mimouni
authors.forename: Robert
authors.initials: R
authors.lastname: Fontonge
authors.forename: Ahmed
authors.initials: A
authors.lastname: Melhaoui
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D029064:Araceae; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D011759:Pyrrolidines / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D011759:Pyrrolidines; C411262:irniine