[Application of hard tissue slicing in research of bone tissue engineering].
OBJECTIVE
To improve the method of hard tissue slicing for research of bone tissue engineering.


METHODS
The method of hard tissue slicing especially the key technique-slice mounting had been studied and improved.


RESULTS
The experiments showed that our research improved techniques in hard tissue slicing not only in keeping the characteristics of hard tissue slicing, easily stain with HE, Masson, immunohistochemistry, and distinguishing the differences among tissues easily, but also in overcoming the shortcoming of issue shedding easy to happen in the general hard tissue slicing.


CONCLUSIONS
The improved techniques in hard tissue slicing is a more useful hard tissue slicing techniques.
Li
Xiuqun
X
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
Deng
Li
L
Luo
Jingcong
J
D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones / Q000166:cytology*; D002478:Cells, Cultured; D005269:Femur / Q000166:cytology; D005456:Fluorescent Dyes; D010006:Osteoblasts; D011817:Rabbits; D011884:Radius / Q000166:cytology; D051381:Rats; D016610:Tissue Embedding; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D005456:Fluorescent Dyes