title: Autosomal localization of interstitial telomeric sites (ITS) in brook trout, Salvelinus fontinalis (Pisces, Salmonidae).
abstract: Cytogenetic analysis of brook trout performed with molecular and conventional methods led to identification of interstitial telomeric sites on one or two subtelocentric chromosomes within the same pair. Morphology and specific patterns of these chromosomes using fluorochromes associated with A/T- or G/C-rich DNA proved that these chromosomes are not sex related. The chromomycin-positive region was located on the short arms of the ITS bearing chromosome pair and flanked by telomeric sequences, suggesting that this part of the chromosome had been translocated from another one. Our observations confirm that GC-rich regions are highly mobile genetic structures, and led to ITS formation on brook trout chromosomes.
authors.affiliation: Department of Evolutionary Genetics, University of Warmia and Mazury, Olsztyn, Poland. kocale@diamant.jouy.inra.fr
authors.forename: K
authors.initials: K
authors.lastname: Ocalewicz
authors.forename: A
authors.initials: A
authors.lastname: Sliwinska
authors.forename: M
authors.initials: M
authors.lastname: Jankun
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D020862:GC Rich Sequence; D008297:Male; D020401:Primed In Situ Labeling; D012730:Sex Chromosomes; D016615:Telomere; D014337:Trout / Q000235:genetics*