title: Molecular analysis of a Measles virus isolate from Brazil: a case originating in Japan.
abstract: This study reports on molecular analysis of a Measles virus (MV) isolate from a patient who was infected in Japan but showed symptoms after arriving to Brazil. This patient had typical clinical measles infection symptoms: fever, rash, cough and coryza. After isolating the virus in B95a cells, a fragment of the nucleoprotein (N) gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subjected to direct nucleotide sequencing. The sequence data showed that the MV isolate of concern is of the D5 genotype.
authors.affiliation: Instituto Adolfo Lutz, São Paulo, Brasil. olive40@uol.com.br
authors.forename: M I
authors.initials: MI
authors.lastname: Oliveira
authors.forename: S P
authors.initials: SP
authors.lastname: Curti
authors.forename: C A
authors.initials: CA
authors.lastname: Figueiredo
authors.forename: A M A
authors.initials: AM
authors.lastname: Sardinha
authors.forename: M A M
authors.initials: MA
authors.lastname: Sallum
authors.forename: E L
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D001938:Brazil; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008457:Measles / Q000453:epidemiology* / Q000821:virology; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
chemical_list: D009698:Nucleoproteins; D012367:RNA, Viral