title: DRF 3188 a novel semi-synthetic analog of andrographolide: cellular response to MCF 7 breast cancer cells.
abstract: BACKGROUND
  We determined the effect of andrographolide and one of its novel semi-synthetic analog, DRF 3188, on the cell cycle of MCF 7 breast cancer cells.
  
  
  METHODS
  The effect of the compounds on cell cycle was determined using FACS and western blot analysis of cell cycle proteins. Hollow fibre assay was used to determine if the compounds had the same effect on the cell cycle in vitro and in vivo.
  
  
  RESULTS
  Our results from the in vitro and in vivo experiments show that both the compounds block the cell cycle at the G0-G1 phase through the induction of the cell cycle inhibitor, p27, and the concomitant decrease in the levels of Cdk4.
  
  
  CONCLUSIONS
  The results show that the novel semi-synthetic analog, DRF3188, and andrographolide bring about the anti cancer activity by a similar mechanism.
authors.affiliation: Discovery Research, Dr, Reddy's Laboratories, Bollaram Road, Miyapur, Hyderabad-500049, India. chitkala@drreddys.com
authors.forename: Chitkala
authors.initials: C
authors.lastname: Satyanarayana
authors.forename: Dhanavanthri S
authors.initials: DS
authors.lastname: Deevi
authors.forename: R
authors.initials: R
authors.lastname: Rajagopalan
authors.forename: Nanduri
authors.initials: N
authors.lastname: Srinivas
authors.forename: Sriram
authors.initials: S
authors.lastname: Rajagopal
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; C488658:DRF3188; D004224:Diterpenes; C030419:andrographolide