Abscisic acid is involved in the water stress-induced betaine accumulation in pear leaves.
ABA exogenously applied to the leaves of the whole plants of pear (Pyrus bretschneideri Redh. cv. Suly grafted on Pyrus betulaefolia Rehd.) significantly increased the betaine concentrations in the leaves when the plants were well watered. The plants subjected to 'drought plus ABA' treatment had significantly higher betaine concentrations in their leaves than those given drought treatment alone. The 'drought plus ABA' treatment increased the amount of betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) and its activity in the leaves more than did the drought treatment alone. The experiments with detached leaves showed that ABA treatment significantly increased the concentration of betaine, activity of BADH and apparent amount of BADH in non-dehydrated leaves, and enhanced the accumulation of betaine, activity of BADH and apparent amount of BADH in dehydrated leaves. These effects of ABA were both time- and dose-dependent. Two ABA isomers, (-)-cis, trans-ABA and 2-trans, 4-trans-ABA, had no effect on the betaine accumulation in the leaves, showing that the ABA-induced effects are specific. These data demonstrate that ABA is involved in the drought-induced betaine accumulation in the pear leaves.
Gao
Xiu-Ping
XP
China State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094.
Pan
Qiu-Hong
QH
Li
Mei-Jun
MJ
Zhang
Ling-Yun
LY
Wang
Xiao-Fang
XF
Shen
Yuan-Yue
YY
Lu
Yan-Fen
YF
Chen
Shang-Wu
SW
Liang
Zheng
Z
Zhang
Da-Peng
DP
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism*; D050728:Betaine-Aldehyde Dehydrogenase; D003681:Dehydration / Q000201:enzymology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007536:Isomerism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D031989:Pyrus / Q000187:drug effects / Q000378:metabolism*; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000502:physiology
D001622:Betaine; D000040:Abscisic Acid; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase