title: Biochemical and morphological characteristics of selenite-induced apoptosis in human hepatoma Hep G2 cells.
abstract: Selenium is a cellular growth inhibitor in many mammary tumor cells. To comprehend the mechanism for the selenium-induced cell death, we examined the effects of sodium selenite, which has been one of the most extensively investigated selenium compounds, in human hepatoma Hep G2 cells.Cell viability gradually decreased after treatment with sodium selenite within the concentration range of 10-50 microM. Low (10 mM) selenite has shown a high-percentage laddering pattern compared to the high (25 microM) cytotoxic selenium concentration in agarose gel electrophoresis. G2/M-phase enrichment was also concentration dependent. The most consistent transmission electron microscopic finding was the existence of large lysosomes. Based on these data, we hypothesize that sodium selenite predominantly shows its apoptotic effect over hydrogen selenite accumulation.
authors.affiliation: Department of Biochemistry, Ege University School of Medicine, Bornova, TR-35100 Izmir, Turkey.
authors.forename: Handan Ak
authors.initials: HA
authors.lastname: Celik
authors.forename: Hikmet Hakan
authors.initials: HH
authors.lastname: Aydin
authors.forename: Remziye
authors.initials: R
authors.lastname: Deveci
authors.forename: Ender
authors.initials: E
authors.lastname: Terzioglu
authors.forename: Sabire
authors.initials: S
authors.lastname: Karacali
authors.forename: Guray
authors.initials: G
authors.lastname: Saydam
authors.forename: Ulus
authors.initials: U
authors.lastname: Akarca
authors.forename: Yucel
authors.initials: Y
authors.lastname: Batur
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011419:Propidium / Q000494:pharmacology; D018038:Sodium Selenite / Q000494:pharmacology*
chemical_list: D006131:Growth Inhibitors; D011419:Propidium; D018038:Sodium Selenite