Lack of a fusion requirement for development of bone marrow-derived epithelia.
Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with beta-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.
Harris
Robert G
RG
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.
Herzog
Erica L
EL
Bruscia
Emanuela M
EM
Grove
Joanna E
JE
Van Arnam
John S
JS
Krause
Diane S
DS
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002459:Cell Fusion; D004179:Cobra Cardiotoxin Proteins / Q000494:pharmacology; D004546:Elapid Venoms / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D049452:Green Fluorescent Proteins; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007633:Keratins / Q000032:analysis; D008164:Luminescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D032342:Muscle Cells / Q000166:cytology; D011839:Radiation, Ionizing; D045522:Recombinases / Q000378:metabolism; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014960:X Chromosome; D014998:Y Chromosome; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D008164:Luminescent Proteins; D045522:Recombinases; D049452:Green Fluorescent Proteins; C005082:notexin; D007633:Keratins; D001616:beta-Galactosidase