title: Solid-phase extraction-high-performance liquid chromatography-ion trap mass spectrometry for analysis of trace concentrations of macrolide antibiotics in natural and waste water matrices.
abstract: A method using solid-phase extraction (SPE) combined with high-performance liquid chromatography-ion trap tandem mass spectrometry (LC-MS-MS) has been developed for determination of trace concentrations of erythromycin-H2O (ETM-H2O), roxithromycin (RTM) and tylosin (TLS) in natural and waste water matrices. These macrolides (MLs) were extracted from water samples using Oasis HLB cartridges, and the average recovery was 93.6 +/- 8.6, 92.1 +/- 10.0, and 94.3 +/- 8.9% for ETM-H2O, RTM and TLS in surface water, respectively. For water from the influent of a wastewater treatment plant (WWTP), the average recovery was 84.8 +/- 14.0, 83.2 +/- 13.1, and 86.1 +/- 13.4% for ETM-H2O, RTM and TLS, respectively. Method detection limits in a natural water matrices were 0.07, 0.03, and 0.05 microg/l for ETM-H2O, RTM, and TLS, respectively. Fragment or product ions from MS spectra using in-source collision-induced dissociation and MS-MS spectra have been identified. The accuracy and day-to-day variation of the method fell within acceptable ranges. The method was evaluated by studying the occurrence of the three macrolides on a river and a WWTP in northern Colorado. None of the antibiotics were detected in the stream except immediately downstream of a WWTP, a result consistent with their presence in the influent and effluent of the treatment facility.
authors.affiliation: Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, CO 80523-1372, USA.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: K H
authors.initials: KH
authors.lastname: Carlson
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004917:Erythromycin / Q000032:analysis*; D018942:Macrolides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D014874:Water Pollutants, Chemical; D004917:Erythromycin