title: Developmental changes of Ni(2+) sensitivity and automaticity in Nkx2.5-positive cardiac precursor cells from murine embryonic stem cell.
abstract: BACKGROUND
  It is controversial which subtypes of T type Ca(2+) channels are implicated in automaticity of cardiac cells during the embryonic period.
  
  
  RESULTS
  The effect of Ni(2+) on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40 micromol/L Ni(2+), which is enough to block Ni(2+)sensitive T type-Ca(2+) channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni(2+) did not show any effects on the automaticity of 50% of the cells in the late stage.
  
  
  CONCLUSIONS
  These results indicate that Ni(2+)-sensitive T-type Ca(2+) channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.
authors.affiliation: Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Tottori, Japan.
authors.forename: Kasumi
authors.initials: K
authors.lastname: Manabe
authors.forename: Junichiro
authors.initials: J
authors.lastname: Miake
authors.forename: Norihito
authors.initials: N
authors.lastname: Sasaki
authors.forename: Hitomi
authors.initials: H
authors.lastname: Furuichi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yano
authors.forename: Einosuke
authors.initials: E
authors.lastname: Mizuta
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hoshikawa
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Fumihito
authors.initials: F
authors.lastname: Tajima
authors.forename: Goshi
authors.initials: G
authors.lastname: Shiota
authors.forename: Eiji
authors.initials: E
authors.lastname: Nanba
authors.forename: Shigetsugu
authors.initials: S
authors.lastname: Ohgi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hidaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Morisaki
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kurata
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Osamu
authors.initials: O
authors.lastname: Igawa
authors.forename: Chiaki
authors.initials: C
authors.lastname: Shigemasa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hisatome
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D047108:Embryonic Development / Q000502:physiology*; D006321:Heart / Q000196:embryology*; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000502:physiology*; D051379:Mice; D009532:Nickel / Q000494:pharmacology*; D011498:Protein Precursors / Q000187:drug effects / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C484469:Nkx2-5 protein, mouse; D011498:Protein Precursors; D014157:Transcription Factors; D009532:Nickel