Interleukin 11 treatment alters the protein content of Galpha(i2) and adipogenic transcription factors in 3T3-L1 adipocytes.
Exposure of fully differentiated 3T3-L1 adipocytes to 5 nM interleukin 11 (IL-11) resulted in an increase (1.9+/-0.5 fold) in the protein content for the heterotrimeric G protein Galpha(i2). This G protein has been suggested to be involved in the control of the insulin responsive glucose transporter (GLUT4) translocation to the plasma membrane. Conversely, IL-11 had no effect on the content of three other G proteins, involved in insulin action. The alteration in Galpha(i2) protein corresponds to and provides a molecular rationale for our previously described IL-11 induced increase in plasma membrane glucose transporter content and increased rate of glucose transport. In addition, treatment with the cytokine altered the protein content of several transcription factors, C/EBPalpha and CHOP-10 decreased while PPARgamma and C/EBPbeta increased. These changes in transcription factor content are consistent with an alteration of phenotype with the cells reverting to an earlier stage of the differentiation process in response to IL-11.
Tenney
Raleigh
R
Department of Biochemistry and Molecular Biology, The Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA.
Pekala
Phillip H
PH
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism*; D051275:Glucose Transporter Type 4; D017370:Interleukin-11 / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D022762:CCAAT-Enhancer-Binding Proteins; C497250:Ddit3 protein, mouse; D051275:Glucose Transporter Type 4; D017370:Interleukin-11; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D047495:PPAR gamma; D011518:Proto-Oncogene Proteins; C495693:Slc2a4 protein, mouse; D014157:Transcription Factors; D051742:Transcription Factor CHOP; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C498016:Gnai2 protein, mouse