title: Cryopreservation of Helianthus tuberosus cell suspension cultures: the effect of preculture treatments on cytoskeletal proteins and transglutaminase activity.
abstract: Helianthus tuberosus cell suspension cultures were subjected to cryopreservation 24h preculture treatments with 0.5M sucrose or mannitol. Extracts were assayed for transglutaminase activity and the level of alpha-tubulin tyrosination. There was a significant reduction (compared with the non-precultured controls) in transglutaminase activity and alpha-tubulin tyrosination state after mannitol preculture treatment, whereas sucrose preculture treatment produced no significant effect. The results suggest that reduced levels of transglutaminase activity and alpha-tubulin tyrosination are associated with a lack of post-thaw recovery observed following mannitol preculture treatment of cell culture suspensions. These activities may represent useful molecular markers of the success of preculture treatments in cryopreservation protocols.
authors.affiliation: School of Science, The Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
authors.forename: W
authors.initials: W
authors.lastname: Harris
authors.forename: P T
authors.initials: PT
authors.lastname: Lynch
authors.forename: A J
authors.initials: AJ
authors.lastname: Hargreave
authors.forename: P L R
authors.initials: PL
authors.lastname: Bonner
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage; D046508:Culture Techniques; D003598:Cytoskeletal Proteins / Q000502:physiology*; D006368:Helianthus / Q000166:cytology* / Q000201:enzymology*; D008353:Mannitol / Q000008:administration & dosage; D008870:Microtubules / Q000502:physiology; D013395:Sucrose / Q000008:administration & dosage; D011503:Transglutaminases / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents; D003598:Cytoskeletal Proteins; D008353:Mannitol; D013395:Sucrose; D011503:Transglutaminases