Alpha-Ketoglutarate dehydrogenase and lipoic acid synthase are important for the functioning of peroxisomes of Saccharomyces cerevisiae.
A method was devised to search for yeast mutants impaired in peroxisome functioning, indicating cross-talk between metabolic pathways. Two mutants were isolated; they are impaired in oleate utilisation and carry mutations in the KGD1 and LIP5 genes encoding the E1 component of the mitochondrial alpha-ketoglutarate dehydrogenase complex and lipoic acid synthase, respectively. The results presented indicate that the Kgd1 and Lip5 proteins are important for the expression of genes encoding peroxisomal matrix proteins, although they are not necessary for the biogenesis of this cellular compartment.
Smaczyńska-de Rooij
Iwona
I
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland. rytka@psd.ibb.waw.pl
Migdalski
Andrzej
A
Rytka
Joanna
J
D042962:Acyl-CoA Oxidase / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics; D020675:Peroxisomes / Q000201:enzymology* / Q000648:ultrastructure; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013466:Sulfurtransferases / Q000235:genetics / Q000378:metabolism*
D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon; D002374:Catalase; D007655:Ketoglutarate Dehydrogenase Complex; D042962:Acyl-CoA Oxidase; D013466:Sulfurtransferases; C116435:lipoic acid synthase