title: Molecular and genetic interactions between STYLOSA and GRAMINIFOLIA in the control of Antirrhinum vegetative and reproductive development.
abstract: STYLOSA (STY) in Antirrhinum and LEUNIG (LUG) in Arabidopsis control the spatially correct expression of homeotic functions involved in the control of floral organ identity. We show here that the sty mutant also displays alteration in leaf venation patterns and hypersensitivity towards auxin and polar auxin transport inhibitors, demonstrating that STY has a more general role in plant development. STY and LUG are shown to be orthologues that encode proteins with structural relation to GRO/TUP1-like co-repressors. Using a yeast-based screen we found that STY interacts with several transcription factors, suggesting that STY, like GRO/TUP1, forms complexes in vivo. Proteins of the YABBY family, characterised by containing a partial HMG domain, represent a major group of such interactors. In vivo association of STY with one of the YABBY proteins, GRAMINIFOLIA (GRAM), is supported by enhanced phenotypic defects in sty gram double mutants, for instance in the control of phyllotaxis, floral homeotic functions and organ polarity. Accordingly, the STY and GRAM protein and mRNA expression patterns overlap in emerging lateral organ primordia. STY is expressed in all meristems and later becomes confined to the adaxial domain and (pro)vascular tissue. This pattern is similar to genes that promote adaxial identity, and, indeed, STY expression follows, although does not control, adaxial fate. We discuss the complex roles of STY and GRAM proteins in reproductive and vegetative development, performed in part in physical association but also independently.
authors.affiliation: Abteilung für Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, 50829 Köln, Germany.
authors.forename: Cristina
authors.initials: C
authors.lastname: Navarro
authors.forename: Nadia
authors.initials: N
authors.lastname: Efremova
authors.forename: John F
authors.initials: JF
authors.lastname: Golz
authors.forename: Roger
authors.initials: R
authors.lastname: Rubiera
authors.forename: Markus
authors.initials: M
authors.lastname: Kuckenberg
authors.forename: Rosa
authors.initials: R
authors.lastname: Castillo
authors.forename: Olaf
authors.initials: O
authors.lastname: Tietz
authors.forename: Heinz
authors.initials: H
authors.lastname: Saedler
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Schwarz-Sommer
mesh_terms: D032161:Antirrhinum / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D035264:Flowers / Q000033:anatomy & histology / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000378:metabolism; D009024:Morphogenesis / Q000502:physiology; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C417768:LUG protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors