title: Impact of polyunsaturated fatty acids on cytoskeletal linkage of L-selectin.
abstract: Polyunsaturated fatty acid [omega-3 polyunsaturated fatty acids (omega-3PUFAs)] incorporation into cell membranes has been shown to have potent anti-inflammatory activity, though the mechanisms involved are only partially characterized. Here, we show that PUFA enrichment of T cell membranes decreased the overall expression of L-selectin as well as a highly conserved epitope on L-selectin that may serve as a marker for optimal protein function. Additionally, PUFA enrichment inhibited L-selectin cytoskeletal association, which is thought to be important for optimal functional activity. In support of this, PUFA enrichment of gammadelta T cell membranes reduced L-selectin-dependent rolling interactions under conditions mimicking physiological flow. Taken together, these data suggest that the anti-inflammatory activity of omega-3 polyunsaturated fatty acids may be due, in part, to a novel effect on L-selectin, namely PUFA reduction or prevention of cytoskeletal association of L-selectin.
authors.affiliation: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA. jeff.leid@nau.edu
authors.forename: Jeff G
authors.initials: JG
authors.lastname: Leid
authors.forename: Mark A
authors.initials: MA
authors.lastname: Jutila
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003599:Cytoskeleton / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes; D015525:Fatty Acids, Omega-3 / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D019041:L-Selectin / Q000276:immunology* / Q000378:metabolism; D036904:Leukocyte Rolling / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology
chemical_list: D000939:Epitopes; D015525:Fatty Acids, Omega-3; D019041:L-Selectin