Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea.
Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S15-SP11b was also co-transcribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.
Shiba
Hiroshi
H
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Park
Jong-In
JI
Suzuki
Go
G
Matsushita
Masanori
M
Nou
Ill-Sup
IS
Isogai
Akira
A
Takayama
Seiji
S
Watanabe
Masao
M
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000378:metabolism; D020131:Genes, Duplicate; D006239:Haplotypes; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; C431526:SP11 protein, Brassica