Respiration-based measurement of lung deposition of a fluorescent dextrane aerosol in a marmoset monkey.
A technique for measurement of respiration-based lung deposition of an aerosol was investigated and subsequently applied in a pilot study with a marmoset monkey. The technique consisted of an aerosol exposure system for a marmoset using a face mask and a previously constructed monkey chair, a method for recovery of fluorescent dextrane from lung material, and respiration measurement of the marmoset by whole-body plethysmography. In the pilot study, a ketamine-anesthetized marmoset was exposed for 20 min to an FITC-dextrane aerosol atmosphere (200 microg/L air, particle size 1.5 microm mass median aerodynamic diameter [MMAD]). It was found that 3.4% of the inhaled aerosol was deposited in the lungs; the aerosol was distributed over the lung lobes with an higher concentration at the distal side.
Bergers
Willem W A
WW
TNO Prins Maurits Laboratory, Division Chemical and Biological Protection, Rijswijk, The Netherlands. bergers@pml.tno.nl
van de Meent-van der Horst
Dory
D
Joosen
Marloes J A
MJ
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D002144:Callithrix; D003911:Dextrans / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D008168:Lung / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D010865:Pilot Projects
D000336:Aerosols; D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate