Distribution of 99mTc-labeled lymphocytes in control and inflamed rats.
We studied the in vivo fate of CD8(+) lymphocytes, of naive (CD8(+)CD45RC(bright)) or memory (CD8(+)CD45RC(dim)) phenotype, injected in syngeneic rats, after their sorting and labeling with [(99m)Tc] HM-PAO. By using the scintigrafic method we showed that memory CD8(+) lymphocytes were able to recirculate into liver and lungs. The same method was also successfully used to in vivo study the homing of total blood lymphocytes obtained from inflamed rats.
Fulgenzi
Alessandro
A
Istituto di Patologia Generale, Universita degli Studi di Milano, Milano, Italy.
Casati
Rosangela
R
Colombo
Fabio Riccardo
FR
Gasparini
Massimo
M
Ferrero
Elisabetta
E
Bondanza
Attilio
A
Gerundini
Paolo
P
Ferrero
Maria Elena
ME
D000818:Animals; D007249:Inflammation / Q000000981:diagnostic imaging* / Q000378:metabolism*; D008214:Lymphocytes / Q000000981:diagnostic imaging*; D008297:Male; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D019690:Technetium Tc 99m Exametazime / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime