Structural and biological characterization of two novel peptides from the venom of the neotropical social wasp Agelaia pallipes pallipes.
The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences: Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH(2). Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH(2). Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL.
Mendes
Maria Anita
MA
CEIS-Department of Biology, IBRC-UNESP (CAT-CEPID, FAPESP), Institute of Immunological Investigations (Millennium Institute-MCT, CNPq), Rio Claro, SP 13506-900, Brazil.
de Souza
Bibiana Monson
BM
Marques
Mauricio Ribeiro
MR
Palma
Mario Sergio
MS
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001938:Brazil; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002630:Chemotactic Factors / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D036341:Intercellular Signaling Peptides and Proteins; D008407:Mast Cells / Q000378:metabolism; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009943:Organophosphorus Compounds; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D014862:Wasp Venoms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014863:Wasps / Q000737:chemistry*
C043517:2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; D000900:Anti-Bacterial Agents; D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; D009943:Organophosphorus Compounds; D010455:Peptides; D014862:Wasp Venoms; C554058:protonectin (1-6), Agelaia pallipes pallipes; C037500:mastoparan