title: Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylation.
abstract: Integrin-filamin binding plays an important role in adhesion-mediated control of the actin cytoskeleton. Here, using the interaction between recombinant fragments from the C-terminus of filamin A and the cytoplasmic tail of integrin beta 7 as a model, we report a negative regulatory role for filamin alternative splicing. Splice variant forms of filamin A lacking a 41-amino acid segment interacted more strongly than full-length fragments. In addition, we provide evidence that phosphorylation of the splice variant region is unlikely to represent the mechanism by which binding is reduced.
authors.affiliation: School of Biological Sciences, 2.205 Stopford Building, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Travis
authors.forename: Arjan
authors.initials: A
authors.lastname: van der Flier
authors.forename: Richard A
authors.initials: RA
authors.lastname: Kammerer
authors.forename: A Paul
authors.initials: AP
authors.lastname: Mould
authors.forename: Arnoud
authors.initials: A
authors.lastname: Sonnenberg
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Humphries
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D003285:Contractile Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D064448:Filamins; D039641:Integrin beta Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments; D010766:Phosphorylation; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D003285:Contractile Proteins; D018076:DNA, Complementary; D064448:Filamins; D039641:Integrin beta Chains; D008840:Microfilament Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C091368:integrin beta7