Tryptophan pyrrole ring cleavage enzymes in placenta.
Tryptophan pyrrole ring cleavage enzymes were assayed in pregnant uterus of mouse. The highest activity was observed at 6.5 days post-coitus (dpc) with a small activity shoulder at 9.5 to 12.5 dpc concepti and placenta. The highest peak at early concepti of 6.5 dpc was coincided with gene expression of tryptophan 2,3-dioxygenase (TDO). And the shoulder from 9.5 dpc was coincided with the expression of indoleamine 2,3-dioxygenase (IDO). These enzymes showed a different inhibitory behavior to a proper IDO inhibitor, 1-methyl tryptophan. In situ hybridization analysis revealed that TDO was expressed in the decidua of the early concepti. This is the first report of the extra hepatic TDO.
Minatogawa
Yohsuke
Y
Department of Biochemistry, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192 Japan. minato@bcc.kawasaki-m.ac.jp
Suzuki
Sachiko
S
Ando
Yoko
Y
Tone
Shigenobu
S
Takikawa
Osamu
O
D000818:Animals; D001483:Base Sequence; D005260:Female; D017403:In Situ Hybridization; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D051379:Mice; D008813:Mice, Inbred ICR; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D014366:Tryptophan Oxygenase / Q000235:genetics / Q000378:metabolism*
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012333:RNA, Messenger; D014364:Tryptophan; D014366:Tryptophan Oxygenase