title: [The induction of differentiation into dendritic cells from HL-60 cells by calcium ionophore].
abstract: OBJECTIVE
  To explore whether the promyelocytic leukemia cell line HL-60 may differentiate into activated dendritic cells (DCs) by A23187, a calcium ionophore.
  
  
  METHODS
  The HL-60 cells were cultured in common medium alone or with various concentrations of A23187 (25-1 600 microg/L) and rhGM-CSF (100 microg/L). After culture for 24-96 hours, the cellular morphological change was observed under light microscope and electron microscope. Surface makers on treated HL-60 cells were analyzed by flow cytometry. The proliferation of allogeneic human T cells was detected by MTT colorimetry.
  
  
  RESULTS
  Under the condition of a suitable dose (200 microg/L) of A23187 treatment of HL-60 cells for 24 hours, the expression of CD83 molecule, a characteristic marker on DCs, was highest. The typical dendritic outgrowth appeared at a time when HL-60 cells were treated with A23187 for 72 hours. However, when HL-60 cells were treated with A23187 for 96 hours, the expressions of CD80 (B7.1), CD86 (B7.2), MHC-class II molecule and CD54 on HL-60 cells reached peak, and marked activation of allogeneic T cells occurred.
  
  
  CONCLUSIONS
  Calcium ionophore A23187 can induce the HL-60 cells to differentiate into activated DCs-like cells.
authors.affiliation: Institute of Tumor Molecular Biology, General Hospital of Guangzhou Command, Guangzhou 510010, China. Junwuwang@hotmail.com
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Tai-cheng
authors.initials: TC
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Xian
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen; D000001:Calcimycin / Q000494:pharmacology*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D000001:Calcimycin; D007136:Immunoglobulins; D018799:Intercellular Adhesion Molecule-1; D007476:Ionophores; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C497964:CD86 protein, human