Exclusion of alcohols from spermidine-DNA assemblies: probing the physical basis of preferential hydration.
The interaction of the alcohols 2-methyl-2,4-pentanediol (MPD) and 2-propanol and of glycerol with condensed spermidine(3+)-DNA arrays are investigated with direct force measurements using osmotic stress coupled with X-ray scattering. Thermodynamic forces between DNA helices are measured from the dependence of helical interaxial spacings on the osmotic pressure applied by poly(ethylene glycol) solutions in equilibrium with the DNA phase. The sensitivity of these forces to solute concentration can be transformed into a change in the number of excess or deficit solutes or waters in the DNA phase by applying the Gibbs-Duhem equation. The alcohols examined are excluded from the condensed DNA array and strongly affect the osmotic stress force curves. DNA is preferentially hydrated. MPD is significantly more excluded than 2-propanol. The exclusion of these alcohols, however, is not due to a steric repulsion since glycerol that is intermediate in size between MPD and 2-propanol does not observably affect DNA force curves. As the distance between DNA helices varies, the change in the number of excess waters is independent of alcohol concentration for each alcohol. These solutes are acting osmotically on the condensed array. The distance dependence of exclusion indicates that repulsive water structuring forces dominate the interaction of alcohols with the DNA surface. The exclusion measured for these condensed arrays can quantitatively account for the effect of these alcohols on the precipitation of DNA from dilute solution by spermidine(3+).
Hultgren
Anne
A
Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Rau
Donald C
DC
D019840:2-Propanol / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry*; D000818:Animals; D011232:Chemical Precipitation; D002645:Chickens; D004247:DNA / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry; D006018:Glycols / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000737:chemistry; D012542:Scattering, Radiation; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*; D014965:X-Rays
D000438:Alcohols; D006018:Glycols; D014867:Water; C007545:DNA-spermidine; D011092:Polyethylene Glycols; D004247:DNA; C011480:hexylene glycol; D019840:2-Propanol; D005990:Glycerol; D013095:Spermidine