Massive excystation of Toxoplasma gondii sporozoites.
In the present study, several stimuli were tested to liberate Toxoplasma gondii sporozoites from oocysts. Incubation in Na(2)CO(3)-CO(2) buffer at 40 degrees C with ultrasound, followed by incubation at 40 degrees C with bile or deoxycholic acid, was the only method that resulted in 95% liberation of motile infective sporozoites. A protocol of reproducibly high efficiency is provided, that compares favorably with any of the methods previously published.
Freyre
Alvaro
A
Laboratory for Toxoplasmosis, Department for Parasitology, College for Veterinary Sciences, Alberto Lasplaces 1550, Montevideo, Uruguay. freyrealvaro@hotmail.com
Falcón
Jesús
J
D000818:Animals; D001646:Bile / Q000737:chemistry; D002245:Carbon Dioxide / Q000494:pharmacology; D002254:Carbonates / Q000494:pharmacology; D002415:Cats; D051379:Mice; D033661:Oocysts / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D034101:Sporozoites / Q000187:drug effects / Q000502:physiology; D014122:Toxoplasma / Q000187:drug effects / Q000502:physiology*; D014465:Ultrasonics
D002254:Carbonates; D002245:Carbon Dioxide; C005686:sodium carbonate