title: Characterization of a point mutation in the parC gene of Mycoplasma bovirhinis associated with fluoroquinolone resistance.
abstract: Quinolone-resistant (QR) mutants of Mycoplasma bovirhinis strain PG43 (type strain) were generated by stepwise selection in increasing concentrations of enrofloxacin (ENR). An alteration was found in the quinolone resistance-determining region (QRDR) of the parC gene coding for the ParC subunit of topoisomerase IV from these mutants, but not in the gyrA, gyrB, and parE gene coding for the GyrA and GyrB subunits of DNA gyrase and the ParE subunit of topoisomerase IV. Similarly, such an alteration in QRDR of parC was found in the field isolates of M. bovirhinis, which possessed various levels of QR. The substitution of leucine (Leu) by serine (Ser) at position 80 of QRDR of ParC was observed in both QR-mutants and QR-isolates. This is the first report of QR based on a point mutation of the parC gene in M. bovirhinis.
authors.affiliation: Clinical Research Center of Animal Health, Meiji Seika Kaisha Ltd, Yokohama, Kanagawa 222-8567.
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: Y
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: K
authors.initials: K
authors.lastname: Kotani
authors.forename: K
authors.initials: K
authors.lastname: Abiko
authors.forename: H
authors.initials: H
authors.lastname: Sato
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D007564:Japan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000187:drug effects / Q000235:genetics*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D017931:DNA Primers; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV