title: Rotavirus infection in children with acute diarrhea as detected by latex agglutination, ELISA and polyacrylamide gel electrophoresis.
abstract: We examined prospectively, stool specimens from 135 children, 0 to 3 years old, referred for fever, abdominal pain, vomiting and/or acute diarrhea. Rotavirus antigens were detected from fecal samples by latex agglutination (LA), ELISA and polyacrylamide gel electrophoresis (PAGE). Rotavirus antigen positivity by Latex, ELISA and PAGE were 15, 55%, 12.59% and 11.85%, respectively. With PAGE test as reference, the sensitivity and specificity of LA and ELISA tests was 93.75%, 94.96% and 100%, 99.16%, respectively, The positivity ratio between 13-24 months group was meaningful with all tests (P = 0.042 for LA; P = 0.05 for ELISA; P = 0.031 for PAGE). ELISA and LA use found to be as sensitive and specific as PAGE in the diagnosis of rotavirus diarrhea.
authors.affiliation: Kocatepe University School of Medicine, Department of Microbiology and Clinic Microbiology, Afyon, Turkey. maltindis@hotmail.com
authors.forename: Mustafa
authors.initials: M
authors.lastname: Altindis
authors.forename: Sibel
authors.initials: S
authors.lastname: Yavru
authors.forename: Atilla
authors.initials: A
authors.lastname: Simsek
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozkul
authors.forename: Ayban
authors.initials: A
authors.lastname: Ceri
authors.forename: Hasan
authors.initials: H
authors.lastname: Koc
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D008297:Male; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012400:Rotavirus Infections / Q000175:diagnosis*; D014421:Turkey