[Effect of different allogenic cells injected into denervated muscles on nerve regeneration in rats].
OBJECTIVE
To study the effect of allogenic different cells injected into denervated muscles on nerve regeneration.


METHODS
Thirty-six adult female SD rats, weighted 120-150 g, were divided into four groups randomly (n = 9, each group). Left sciatic nerves were cut down on germfree conditions and given primary suture of epineurium. Different cells were injected into the muscles of calf at once after operation every seven days and in all four times (group A: 1 ml Schwann cells at concentration of 1 x 10(6)/ml; group B: 1 ml mixed cells of Schwann cells and myoblast cells at concentration of 1 x 10(6)/ml; group C: 1 ml extract from the culture medium of kidney endothelial cells; and group D: 1 ml culture medium without FCS as control). After 3 months, the specimen was observed on macrobody and histology, and the densities of neurilemma cell and myoceptor were counted.


RESULTS
The means of proximate neurilemma cells were 0.1877 +/- 0.0542 in group A, 0.1551 +/- 0.0321 in group B, 0.0724 +/- 0.0237 in group C, and 0.1877 +/- 0.0542 in group D. The densities of myoceptor were 6.000 +/- 0.866 in group A, 9.000 +/- 2.291 in group B, 12.780 +/- 1.394 in group C, 3.110 +/- 0.782 in group D.


CONCLUSIONS
Schwann cells, mixed cells of Schwann cells with myoblast cells, and the extract from kidney endothelial cells can all accelerate the nerve regeneration. And the effect of extract from the kidney endothelial cell is superior to that of Schwann cell and mixed cell.
Liu
Minghui
M
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
Yang
Zhiming
Z
Xie
Huiqi
H
D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D007273:Injections, Intramuscular; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation* / Q000473:pathology; D032446:Myoblasts / Q000637:transplantation; D009416:Nerve Regeneration; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000637:transplantation; D012584:Sciatic Nerve