Adjacent cysteines are capable of ligating the same tetranuclear iron-sulfur cluster.
The mechanism of the energy-converting NADH (beta-nicotinamide adenine dinucleotide, reduced form):ubiquinone oxidoreductase, which is also called respiratory complex I, is largely unknown due to lack of a high-resolution structure and the most complicated construction of the enzyme. Electron transport is carried out by one flavin mononucleotide (FMN) and up to 9 Fe/S clusters. The Fe/S cluster N2, which is believed to be directly involved in redox-coupled proton-translocation, is located on subunit NuoB (the homologue of the mitochondrial PSST subunit). This subunit contains a conserved binding motif for a [4Fe/4S] cluster with two adjacent cysteines. It was questioned whether these adjacent cysteines could be ligands of the same cluster due to a possible steric hinderance. However, mutagenesis of either of these cysteines led to a loss of cluster N2. We used the known structure of the homologous small subunit of hydrogenases containing a regular cysteine motif to generate an in silico mutant with two consecutive cysteines. Molecular dynamics simulation showed that the conformation of these cysteines does not meet the topological requirements for coordination of a [4Fe/4S] cluster when the protein backbone conformation is kept constant. In comparison, the simulation of a dipeptide amide using a "template forcing" approach resulted in a conformation compatible to an optimal coordination of the two cluster positions in question. Thus, a slight main-chain conformational change would allow two adjacent cysteines to coordinate a [4Fe/4S] cluster.
Gurrath
Marion
M
Institut für Pharmazeutische Chemie, Universität Düsseldorf, Düsseldorf, Germany.
Friedrich
Thorsten
T
D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D001665:Binding Sites; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry*; D045286:Desulfovibrio gigas / Q000201:enzymology; D016969:Desulfovibrio vulgaris / Q000201:enzymology; D042967:Electron Transport Complex I / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry*; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D008024:Ligands; C043296:nickel-iron hydrogenase; D006864:Hydrogenase; D042967:Electron Transport Complex I; D003545:Cysteine