[Purification of plasmin-alpha2-antiplasmin complex by lysine-sepharose 4B affinity chromatography employing sequential elutions].
To easily obtain plasmin-alpha(2)-antiplasmin complex (PAP) with high purity, the purification procedure was improved by authors. After urokinase activated fresh human platelet-deficient plasma had been applied to Lysine-Sepharose 4B affinity chromatography column, elutions were sequentially performed by several eluents with different ingredients. The results showed that 3.0 mg PAP could harvested from 100 ml fresh plasma by this method and the whole procedure could be finished within several hours. In conclusion, this procedure is simple, rapid, economical and high-yield.
Li
Jian-Xin
JX
Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China.
Zhou
Yun
Y
Wang
Hong
H
Zheng
Zuo-Ya
ZY
Li
Dao
D
Wang
Hong-Li
HL
D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D012685:Sepharose / Q000031:analogs & derivatives*; D000979:alpha-2-Antiplasmin / Q000032:analysis / Q000302:isolation & purification*
D000979:alpha-2-Antiplasmin; C030675:lysine-sepharose; C037742:plasmin-plasmin inhibitor complex; D012685:Sepharose; D005341:Fibrinolysin