[Allele polymorphism of alkaline phosphatase, acid soluble phosphatase, and vitamin D-binding protein genes in postmenopausal osteoporosis].
OBJECTIVE
To study polymorphism of genes involved in mechanisms regulating metabolism of bone tissue: alkaline (ALPL) and acid (ACP1) phosphatases, vitamin D-binding protein (GC); to ascertain associations of their genotypes and alleles with osteoporosis (OP) and mineral density of spinal and femoral bone tissue (BTMD).


METHODS
Relevant genetic examination was made in 70 females with OP diagnosed by the WHO criteria (1994) aged 60-79 years (mean age 71.0 +/- 6.2 years) and 51 OP-free females in the same age interval (mean age 69.0 +/- 5.6 years). Polymorphic sites of the genes were examined by polymerase chain reaction. Trinucleotide repeat, ARG105GLN polymorphism of restrictive fragment length (PRFL), [GC, TRH420LYS] PRFL were studied for ALPL gene, ACP1 gene and GC gene, respectively.


RESULTS
Association was found between frequencies of genotypes SS, 2F and FS, F allele of GC gene with OP as well as between PRFL of the spine, femur and some GC genotypes in OP women. Genes ALPL and ACP1 were not associated with OP.


CONCLUSIONS
It is suggested that genotypes SS, 2F and FS have marked functional differences in fixation and transport of vitamin D active metabolites involved in metabolism of bone tissue in OP.
Krylov
M Iu
MIu
Korotkova
T A
TA
Miakotkin
V A
VA
Benevolenskaia
L I
LI
D015502:Absorptiometry, Photon; D000368:Aged; D000469:Alkaline Phosphatase / Q000235:genetics*; D015519:Bone Density / Q000235:genetics; D005260:Female; D005272:Femur Neck / Q000000981:diagnostic imaging; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000000981:diagnostic imaging / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013131:Spine / Q000000981:diagnostic imaging; D014809:Vitamin D-Binding Protein / Q000235:genetics*
D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D014809:Vitamin D-Binding Protein; D000469:Alkaline Phosphatase; C490429:ACP1 protein, human; D017027:Protein Tyrosine Phosphatases