title: A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing.
abstract: Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.
authors.affiliation: Laboratorio Fisiología Vegetal, Departamento BOS, Facultad de Biología, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n. E-33071, Oviedo, Spain.
authors.forename: L Borja
authors.initials: LB
authors.lastname: Diego
authors.forename: María
authors.initials: M
authors.lastname: Berdasco
authors.forename: Mario F
authors.initials: MF
authors.lastname: Fraga
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Cañal
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Carmen
authors.initials: C
authors.lastname: Castresana
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D028223:Pinus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D046988:Proteasome Endopeptidase Complex; D018749:RNA, Plant / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D009097:Multienzyme Complexes; D018749:RNA, Plant; D012335:RNA, Ribosomal; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases