A monoclonal antibody to feruloylated-(1-->4)-beta-D-galactan.
We report the isolation and characterization of a monoclonal antibody, designated LM9, against feruloylated-(1-->4)-beta-D-galactan. This epitope is a structural feature of cell wall pectic polysaccharides of plants belonging to the family Amaranthaceae (including the Chenopodiaceae). Immuno-assays and immunofluorescence microscopy indicated that LM9 binding is specific to samples and cell walls obtained from species belonging to this family. In a series of competitive-inhibition enzyme-linked immunosorbent assays with potential oligosaccharide haptens, the most effective inhibitor was O-[6-O-(trans-feruloyl)-beta-D-galactopyranosyl]-(1-->4)-D-galactopyranose (Gal2F). LM9 is therefore a useful antibody probe for the analysis of phenolic substitution of cell wall pectic polymers and of cell wall structure in the Amaranthaceae including sugar beet (Beta vulgaris L.) and spinach (Spinacia oleracea L.).
Clausen
Mads H
MH
Centre for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Ralet
Marie-Christine
MC
Willats
William G T
WG
McCartney
Lesley
L
Marcus
Susan E
SE
Thibault
Jean-François
JF
Knox
J Paul
JP
D027703:Amaranthaceae / Q000737:chemistry* / Q000276:immunology* / Q000648:ultrastructure; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D027461:Beta vulgaris / Q000276:immunology; D002473:Cell Wall / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005685:Galactans / Q000032:analysis* / Q000276:immunology*; D016015:Logistic Models; D010368:Pectins / Q000032:analysis; D010944:Plants / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005685:Galactans; D010368:Pectins