[Plasma ascorbic acid: preanalytical factors and new HPLC method].
We describe a fast ED-HPLC method for ascorbic acid analysis, carried out on a new mixed mode chromatographic column. Due to its functional dodecylsilane group associating a quaternary ammonium, this column is simpler and easier to handle than previous ones because without any counter-ion in the mobile phase. As ascorbic acid and glutathione are very often measured on the same blood sample, it was efficient to validate a common pretreatment solution for ascorbic acid in plasma and glutathione in whole blood. This pretreatment is based on the use of a sulfosalycilic acid, N-ethylmaleimide, EDTA mix recently described. Plasma samples, stored after deproteinisation and without centrifugation, are stable for three weeks at -20 or -80 degrees C.
Flourié
F
F
Fédération de biochimie, Hôpital Edouard Herriot, 3, place d'Arsonval, 69437 Lyon. francoise.floury@chu-lyon.fr
Steghens
J-P
JP
D001205:Ascorbic Acid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid
D001205:Ascorbic Acid