title: Use of inherently conducting polymers and pulsed amperometry in flow injection analysis to detect oligonucleotides.
abstract: Pulsed amperometry has been used to detect and discriminate between different base-pair oligonucleotides using flow injection analysis with polypyrrole modified electrodes. Oligonucleotides with 20-repeat units were immobilized as the sole counter anion during polymerisation of pyrrole onto a glassy carbon (GC) electrode. The modified electrode was then used in a flow injection analysis (FIA) set-up to selectively detect complementary oligonucleotides. Results showed that complementary oligo(dG)(20) and non-complementary oligonucleotides can be distinguished by using the oligo(dC)(20) modified electrode.
authors.affiliation: ARC Centre for Nanostructured Electromaterials, Intelligent Polymer Research Institute, University of Wollongong, NSW 2522, Australia.
authors.forename: Yanzhe
authors.initials: Y
authors.lastname: Wu
authors.forename: Simon E
authors.initials: SE
authors.lastname: Moulton
authors.forename: Chee O
authors.initials: CO
authors.lastname: Too
authors.forename: Gordon G
authors.initials: GG
authors.lastname: Wallace
authors.forename: Dezhi
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans
chemical_list: D004247:DNA