title: Altered calcium-induced exocytosis in neutrophils from allergic patients.
abstract: We have investigated the exocytotic characteristics of neutrophils from allergic patients and healthy volunteers employing the whole cell membrane capacitance (Cm) measurement. The mean serum IgE level from allergic patients (423.75 +/- 12.75 IU/ml) determined by chemiluminescence immunoassay was much higher than that of healthy volunteers (28.47 +/- 16.68 IU/ml). Intracellular dialysis of buffered Ca2+ and GTPgammaS triggered biphasic exocytosis. The total capacitance increment displayed a steep dependence on pipette free Ca2+ concentration ([Ca2+]p), with maximal stimulation achieved at 10 microM. A significant decrease in the total capacitance increment was observed in the allergic group at [Ca2+]p >10 microM. Moreover, at submaximal stimulatory [Ca2+]p of 1 microM, the maximal rate of exocytosis in allergic patients (Vmax = 20.75 +/- 6.19 fF/s) was much faster than that of the healthy control group (Vmax = 7.97 +/- 2.49 fF/s). On the other hand, the Ca2+-independent exocytosis stimulated by GTPgammaS displayed no significant difference in either the total membrane capacitance increments or the maximal rate of exocytosis. The results suggest that hypersecretion of neutrophils in allergic diseases may involve the development of abnormal Ca2+-dependent exocytosis.
authors.affiliation: Institute of Biophysics and Biochemistry, School of Life Science and Technology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Liu
authors.forename: Jicheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wu
authors.forename: Chunguang
authors.initials: C
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000918:Antibody Specificity / Q000187:drug effects; D001249:Asthma / Q000097:blood* / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood; D002118:Calcium / Q000008:administration & dosage*; D018925:Chemokines / Q000378:metabolism; D002681:China; D003571:Cytochalasin B / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005089:Exocytosis / Q000187:drug effects*; D005260:Female; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000008:administration & dosage; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects; D008297:Male; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000187:drug effects; D017455:Receptors, IgE / Q000096:biosynthesis / Q000187:drug effects; D012221:Rhinitis, Allergic, Perennial / Q000097:blood* / Q000503:physiopathology*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D018925:Chemokines; D019707:Receptors, Chemokine; D017455:Receptors, IgE; D007073:Immunoglobulin E; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D003571:Cytochalasin B; D002118:Calcium