Determination and regulation of the migration window in electrokinetic chromatography.
The determination of the velocities of the mobile and the pseudostationary phases (the migration (time) window) is mandatory for the determination of physicochemical properties by electrokinetic chromatography (EKC). This review offers a detailed discussion on the definition, the importance, the determination and the regulation of the migration (time) window in EKC. An overview on the theoretical treatment of chromatographic processes in EKC is given defining EKC in comparison to the term capillary electrophoresis. Methods to determine and influence the migration window are discussed with emphasis on measures that have been taken to modify the electroosmotic flow velocity. Pseudostationary phases (or separation carriers) that are taken into consideration are anionic and cationic micelles, mixed micelles, microdroplets (microemulsions), polymeric pseudostationary phases and dendrimers.
Pyell
Ute
U
Philipps- Universität Marburg, Fachbereich Chemie, Hans-Meerwein-Strasse, D-35032 Marburg, Germany. pyell@chemie.uni-marburg.de
D000838:Anions; D002412:Cations; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods*; D004655:Emulsions; D007202:Indicators and Reagents; D008433:Mathematics; D008823:Micelles; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents
D000838:Anions; D002412:Cations; D004655:Emulsions; D007202:Indicators and Reagents; D008823:Micelles; D011108:Polymers; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate