Nanometric protein arrays on protein-resistant monolayers on silicon surfaces.
We present a novel approach for preparation of nanometric protein arrays, based on binding of avidin molecules to nanotemplates generated by conductive AFM lithography on robust oligo(ethylene glycol)-terminated monolayers on silicon (111) surfaces that are protein-resistant. We showed that only biotinated-BSA but not the native BSA bind to the avidin arrays and that the resulting arrays of biotinated BSA could bind avidin to form protein dots with a feature size of approximately 30 nm. This result demonstrates that the avidin array may serve as templates for preparation of nanoarrays of a wide variety of biotin-tagged proteins for studying their interactions with other protein molecules at nanoscale.
Gu
Jianhua
J
Department of Chemistry and Center for Materials Chemistry, University of Houston, Houston, TX 77204, USA.
Yam
Chi Ming
CM
Li
Sha
S
Cai
Chengzhi
C
D001360:Avidin / Q000737:chemistry*; D036103:Nanotechnology; D011092:Polyethylene Glycols / Q000737:chemistry*; D040081:Protein Array Analysis; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D001360:Avidin; D011092:Polyethylene Glycols; D012822:Silicon Dioxide