Maternal exposure to low doses of bisphenol a has no effects on development of female reproductive tract and uterine carcinogenesis in Donryu rats.
Effects of maternal exposure to low doses of bisphenol A (BPA), including those comparable with human exposure levels, on growth and development of the female reproductive system and uterine carcinogenesis in Donryu rats were investigated. Dams were administered BPA (0, 0.006 and 6 mg/kg/day) daily by gavage from gestation day 2 up to the day before weaning (postnatal day 21 at offspring). The serum levels of BPA were significantly elevated in the dams receiving 6 mg/kg/day, however, BPA levels in the milk of dams, and those in the serum and liver of offspring were similar between control and treated groups. The treatment did not exert any influences on uterine development including weight, gland genesis and estrogen receptor alpha expression, vaginal opening and gonadotropin secretion in the female offspring up to puberty. After maturation, no effects were evident with regard to estrous cyclicity in female offspring treated with BPA. In addition, the treatment had no effects on age-related morphological changes of the reproductive and endocrine organs and uterine carcinogenesis until 15 months of age. The results demonstrate that maternal exposure to BPA at levels comparable to human exposure did not have any effects on the female reproductive system of offspring in rats. In addition, BPA was also found in the serum, milk and liver of control dams and pups, and low levels of BPA were detected in drinking water and pellet diet. The present study showed that the experimental animals were also exposed to environmental BPA in the animal room.
Yoshida
Midori
M
Department of Pathology, Sasaki Institute, Tokyo, Japan. midoriy@sasaki.or.jp
Shimomoto
Takasumi
T
Katashima
Sayumi
S
Watanabe
Gen
G
Taya
Kazuyoshi
K
Maekawa
Akihiko
A
D000818:Animals; D001559:Benzhydryl Compounds; D002273:Carcinogens; D047628:Estrogen Receptor alpha / Q000096:biosynthesis; D004968:Estrogens, Non-Steroidal / Q000633:toxicity; D005260:Female; D005836:Genitalia, Female / Q000187:drug effects*; D006062:Gonadotropins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure; D009929:Organ Size; D010636:Phenols / Q000097:blood / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D013997:Time Factors; D014594:Uterine Neoplasms / Q000139:chemically induced / Q000209:etiology*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002273:Carcinogens; D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal; D006062:Gonadotropins; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds