[Expression of the integrin alpha 6 beta 4 and effects of integrin alpha 6 beta 4 on adhesive and invasive behaviors of human laryngeal carcinoma cell lines].
OBJECTIVE
To study the expression of integrin alpha 6 beta 4 in human laryngeal carcinoma cell lines Hep-2 and AMC-HN8. To explore the mechanism and the effects of integrin alpha 6 beta 4 in human laryngeal carcinoma cell lines.


METHODS
Flowcytometry was used to exame the surface expression of integrin alpha 6 beta 4 in human laryngeal carcinoma cell lines. Anti-alpha 6 and anti-beta 4 specific monoclonal antibody were used in adherence inhibition assay and in vitro invasion assay.


RESULTS
Overexpression of integrin alpha 6 beta 4 were present in both Hep-2 and AMC-HN8 cell lines. Anti-alpha 6 and anti-beta 4 specific monoclonal antibody together blocked the alpha 6 beta 4 on the surface of the cells resulting in low adherence on the extracellular matrix and weak invasion through the extracellular matrix.


CONCLUSIONS
Integrin alpha 6 beta 4 play a vitral role in the adhension and invasion of laryngeal carcinoma. Anti-alpha 6 and anti-beta 4 specific monoclonal antibody together can block the function of integrin alpha 6 beta 4. So treatment with anti-alpha 6 and anti-beta 4 specific monoclonal antibody may reduce the development of laryngeal carcinoma.
Zhou
Jiaqing
J
Department of Otorhinolaryngology-Head and Neck Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai, 200001, China.
Zhou
Liang
L
Jin
Xiaojie
X
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005109:Extracellular Matrix; D006801:Humans; D039161:Integrin alpha6beta4 / Q000096:biosynthesis* / Q000502:physiology; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness
D000911:Antibodies, Monoclonal; D039161:Integrin alpha6beta4