title: Purification and crystallization of the yeast translation elongation factor eEF3.
abstract: A Saccharomyces cerevisiae strain expressing full-length histidine-tagged translation elongation factor 3 (eEF3) as the only form of the protein facilitated purification of the factor for both structural and functional studies. Additionally, an identical full-length form has been successfully expressed in Escherichia coli and a C-terminally truncated form of histidine-tagged eEF3 has been successfully expressed in E. coli and S. cerevisiae. Both forms have been crystallized and crystals of the truncated protein expressed in yeast diffract synchrotron radiation to a maximum resolution of 2.3 A. A density-modified map derived from low-resolution SIRAS phases allows model building.
authors.affiliation: Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus, Denmark.
authors.forename: Christian Folsted
authors.initials: CF
authors.lastname: Andersen
authors.forename: Monika
authors.initials: M
authors.lastname: Anand
authors.forename: Thomas
authors.initials: T
authors.lastname: Boesen
authors.forename: Lan Bich
authors.initials: LB
authors.lastname: Van
authors.forename: Terri Goss
authors.initials: TG
authors.lastname: Kinzy
authors.forename: Gregers Rom
authors.initials: GR
authors.lastname: Andersen
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae