Influence of histamine and H1-receptor antagonists on ejaculated human spermatozoa: role of intrasperm Ca2+.
Histamine reduced sperm viability in a dose- and time-dependent manner, accompanied by rise in intrasperm Ca2+. Further, 2',4'-dichlorobenzamil hydrochloride (DBZ), a Na+-Ca2+ exchange inhibitor, known to elevate intrasperm Ca2+, potentiated both, elevation of intrasperm Ca2+ and spermicidal action of histamine. Pretreatment of sperm with very low doses of H1-receptor antagonists (chlorpheniramine, promethazine or diphenhydramine) prevented the histamine-induced elevation of intrasperm Ca2+ as well as its spermicidal action. However, pretreatment with famotidine, a H2-receptor antagonist did not produce such a protective action. The results strongly suggest that histamine elicits its spermicidal action via H1-receptors present on sperm cells.
Gupta
A
A
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147 002, India.
Khosla
R
R
Gupta
S
S
Tiwary
A K
AK
D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival; D002744:Chlorpheniramine / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004542:Ejaculation; D006632:Histamine / Q000378:metabolism*; D006633:Histamine Antagonists / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011398:Promethazine / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013997:Time Factors
D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D002744:Chlorpheniramine; D006632:Histamine; D004155:Diphenhydramine; D011398:Promethazine; D002118:Calcium