title: The in vitro quality of red blood cells frozen with 40 percent (wt/vol) glycerol at -80 degrees C for 14 years, deglycerolized with the Haemonetics ACP 215, and stored at 4 degrees C in additive solution-1 or additive solution-3 for up to 3 weeks.
abstract: BACKGROUND
  Red blood cells (RBCs) frozen with 40 percent (wt/vol) glycerol, stored at -80 degrees C (mean temperature; range, -65 to -90 degrees C) for 14 years, deglycerolized in the Haemonetics automated cell processor (ACP) 215 with the 325-mL disposable bowl, and stored at 4 degrees C in additive solution (AS)-1 or AS-3 for 21 days were evaluated.
  
  
  METHODS
  A total of 106 units of citrate phosphate dextrose adenine-1 RBCs were frozen with 40 percent (wt/vol) glycerol in the original 800-mL polyvinylchloride plastic bag and stored in corrugated cardboard boxes at -80 degrees C for 14 years. The thawed units were deglycerolized with the ACP 215 with a 325-mL disposable bowl and stored in AS-1 or AS-3 at 4 degrees C for 21 days.
  
  
  RESULTS
  The freeze-thaw recovery value was 94 +/- 4 percent (mean +/- SD), the freeze-thaw-wash recovery value was 80 +/- 7 percent, and there was no breakage. Thirty-eight units were processed as 19 pairs. Two units of ABO-matched units were thawed, pooled, divided equally into two units, and deglycerolized. One unit was stored in AS-1 and the other in AS-3 at 4 degrees C for 21 days. Units stored in AS-1 exhibited significantly greater hemolysis than those stored in AS-3.
  
  
  CONCLUSIONS
  Acceptable results were achieved when RBCs frozen at -80 degrees C for 14 years were deglycerolized in the ACP 215. Deglycerolized RBCs in AS-1 exhibited significantly higher hemolysis than those in AS-3 after storage at 4 degrees C for 7 to 21 days.
authors.affiliation: Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: C Robert
authors.initials: CR
authors.lastname: Valeri
authors.forename: Rithy
authors.initials: R
authors.lastname: Srey
authors.forename: Daniel
authors.initials: D
authors.lastname: Tilahun
authors.forename: Gina
authors.initials: G
authors.lastname: Ragno
mesh_terms: D001793:Blood Preservation; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000302:isolation & purification*; D017707:Erythrocyte Transfusion; D005990:Glycerol / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol