title: In vitro growth inhibition of major mastitis pathogens by Staphylococcus chromogenes originating from teat apices of dairy heifers.
abstract: Earlier field observations suggest that teat apex colonization by Staphylococcus chromogenes pre-partum in dairy heifers protects udder quarters against elevated somatic cell counts early post-partum. To explain these findings, the in vitro inhibitory capability of S. chromogenes from teat apices of heifers towards some major mastitis pathogens was tested using a modified cross-streaking method. Two out of 10 S. chromogenes isolates, both originating from two different teats from the same heifer, consistently inhibited growth of all Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis strains, but none of the Escherichia coli strains. The present study, therefore, supports the protective effect of teat apex colonization by S. chromogenes by in vitro production of inhibitory substances.
authors.affiliation: Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. sarne.devliegher@ugent.be
authors.forename: S
authors.initials: S
authors.lastname: De Vliegher
authors.forename: G
authors.initials: G
authors.lastname: Opsomer
authors.forename: A
authors.initials: A
authors.lastname: Vanrolleghem
authors.forename: L A
authors.initials: LA
authors.lastname: Devriese
authors.forename: O C
authors.initials: OC
authors.lastname: Sampimon
authors.forename: J
authors.initials: J
authors.lastname: Sol
authors.forename: H W
authors.initials: HW
authors.lastname: Barkema
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: A
authors.initials: A
authors.lastname: de Kruif
mesh_terms: D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000517:prevention & control; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000382:microbiology* / Q000517:prevention & control; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000254:growth & development; D013290:Streptococcal Infections / Q000382:microbiology* / Q000517:prevention & control; D013291:Streptococcus / Q000254:growth & development*