title: Acute VEGF effect on solute permeability of mammalian microvessels in vivo.
abstract: To investigate the effect of vascular endothelial growth factor (VEGF) on solute permeability of mammalian microvessels, we measured the apparent permeability (P) of various-sized solutes on the postcapillary venules of rat mesentery in vivo. Exposure to 1 nM VEGF transiently increased P from a mean of 1.4 (+/-0.11 SE, n = 17) to a peak of 2.8 (+/-0.28 SE) x 10(-5) cm/s, a 2.4-fold increase for small solute sodium fluorescein (Stokes radius 0.45 nm), from a mean of 0.44 (+/-0.05 SE, n = 16) to a peak of 1.5 (+/-0.19 SE) x 10(-)5 cm/s, a 3.6-fold increase for intermediate-sized solute alpha-lactalbumin (Stokes radius 2.01 nm), from a mean of 0.049 (+/-0.0032 SE, n = 16) to a peak of 0.36 (+/-0.032 SE) x 10(-5) cm/s, a 7.9-fold increase for large solute bovine serum albumin (Stokes radius 3.55 nm), within 30 s. In approximately 2 min, all increased P returned to the baseline values. The response pattern of P to VEGF and the ratios of the peak to control values for rat mesenteric microvessels are similar to those of frog mesenteric microvessels [Am. J. Physiol.: Heart Circ. Physiol. 284 (2003) H2124]. Instead of considerable heterogeneity in the frog mesenteric microvessels, the acute response to 1 nM VEGF is homogeneous in the rat mesenteric microvessels.
authors.affiliation: Department of Mechanical Engineering, University of Nevada-Las Vegas, Las Vegas, NV 89154, USA. bmfu@nscee.edu
authors.forename: Bingmei M
authors.initials: BM
authors.lastname: Fu
authors.forename: Shang
authors.initials: S
authors.lastname: Shen
mesh_terms: D000818:Animals; D002138:Calibration; D004058:Diffusion; D005260:Female; D019793:Fluorescein / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D006403:Hematologic Tests; D007552:Isotonic Solutions / Q000494:pharmacology; D007768:Lactalbumin / Q000378:metabolism; D008833:Microcirculation; D008853:Microscopy; D008856:Microscopy, Fluorescence; D010539:Permeability; D011898:Ranidae; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077331:Ringer's Solution; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D007552:Isotonic Solutions; D042461:Vascular Endothelial Growth Factor A; D012710:Serum Albumin, Bovine; D000077331:Ringer's Solution; D007768:Lactalbumin; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein