title: Human immunodeficiency virus 1 downregulates cell surface expression of the non-classical major histocompatibility class I molecule HLA-G1.
abstract: Human immunodeficiency virus 1 (HIV-1) downregulates cell surface expression of HLA-A and HLA-B but not HLA-C or HLA-E to ultimately escape immune defences. Here, it is shown that cell surface expression of the non-classical HLA-G1 is also downregulated by HIV-1, by using co-transfection experiments and infection with cell-free HIV-1 of HLA-G1-expressing U87 glioma cells or macrophages in primary culture. Moreover, co-transfection experiments using proviruses deleted in either nef or vpu or plasmids encoding HIV-1 Nef and Vpu mixed together with a HLA-G1-expressing construct demonstrated that HLA-G1 downregulation is Nef-independent and Vpu-dependent, contrasting with the Nef- and Vpu-dependent HLA-A2 downregulation. Together, these results show that the decrease of HLA-A2 and HLA-G1 caused by HIV-1 occurs through distinct mechanisms.
authors.affiliation: Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Derrien
authors.affiliation: INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pizzato
authors.affiliation: Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Dolcini
authors.affiliation: INSERM U131, 32 rue des Carnets, 92140 Clamart, France.|Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Menu
authors.affiliation: INSERM U131, 32 rue des Carnets, 92140 Clamart, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Chaouat
authors.affiliation: INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Lenfant
authors.affiliation: Unité de Biologie des Rétrovirus, Département de Virologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Barré-Sinoussi
authors.affiliation: INSERM U563, Centre de Physiopathologie de Toulouse Purpan, 31059 Toulouse Cedex 3, France.
authors.forename: Philippe Le
authors.initials: PL
authors.lastname: Bouteiller
mesh_terms: D000918:Antibody Specificity; D002460:Cell Line; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology
chemical_list: D006680:HLA Antigens; D015789:HLA-A2 Antigen; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D007132:Immunoglobulin Isotypes