Use of inherently conducting polymers and pulsed amperometry in flow injection analysis to detect oligonucleotides.
Pulsed amperometry has been used to detect and discriminate between different base-pair oligonucleotides using flow injection analysis with polypyrrole modified electrodes. Oligonucleotides with 20-repeat units were immobilized as the sole counter anion during polymerisation of pyrrole onto a glassy carbon (GC) electrode. The modified electrode was then used in a flow injection analysis (FIA) set-up to selectively detect complementary oligonucleotides. Results showed that complementary oligo(dG)(20) and non-complementary oligonucleotides can be distinguished by using the oligo(dC)(20) modified electrode.
Wu
Yanzhe
Y
ARC Centre for Nanostructured Electromaterials, Intelligent Polymer Research Institute, University of Wollongong, NSW 2522, Australia.
Moulton
Simon E
SE
Too
Chee O
CO
Wallace
Gordon G
GG
Zhou
Dezhi
D
D000818:Animals; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans
D004247:DNA