CD4(-)c-kit(-)CD3epsilon(-)IL-2Ralpha(+) Peyer's patch cells are a novel cell subset which secrete IL-5 in response to IL-2: implications for their role in IgA production.
In this study, we examined which cell population contributes to IL-5 production by Peyer's patch (PP) cells. Thy1.2(-) fraction of PP cells, but not those of splenocytes, secreted IL-5 in response to IL-2. We found that CD3epsilon(-)IL-2Ralpha(+) cells purified from the Thy1.2(-)B220(-) fraction of PP cells secreted IL-5 when stimulated with IL-2. CD3epsilon(-)IL-2Ralpha(+) cells were subdivided into CD4(+) and CD4(-) populations or c-kit(+) and c-kit(-) populations, and only the CD4(-) and c-kit(-) CD3epsilon(-)IL-2Ralpha(+) cells secreted IL-5 in response to IL-2. CD3epsilon(-)IL-2Ralpha(+) cells did not express NK cell-markers and exhibited a lymphoid morphology. We have therefore identified CD3epsilon(-)IL-2Ralpha(+) cells as a unique lymphoid population that are not classified into conventional IL-5-producing cell populations, such as T cells, mast cells and NK cells. Depletion of CD3epsilon(-)IL-2Ralpha(+) cells from PP resulted in reduced IL-5 production. Furthermore, IgA secretion by B cells was increased when PP B cells were cocultured with CD3epsilon(-)IL-2Ralpha(+) cells. Taken together, these results suggest that the novel subset of CD4(-)c-kit(-)CD3epsilon(-)IL-2Ralpha(+) PP cells are capable of secreting a high level of IL-5 in response to IL-2, contribute markedly to IL-5 production and help IgA secretion by B cells.
Kuraoka
Masayuki
M
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Hashiguchi
Masaaki
M
Hachimura
Satoshi
S
Kaminogawa
Shuichi
S
D000818:Animals; D017252:CD3 Complex / Q000032:analysis / Q000235:genetics; D015704:CD4 Antigens / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005434:Flow Cytometry; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology*; D053645:Interleukin-2 Receptor alpha Subunit; D015848:Interleukin-5 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000235:genetics; D018123:Receptors, Interleukin / Q000378:metabolism
D017252:CD3 Complex; D015704:CD4 Antigens; C508018:Il2ra protein, mouse; D007070:Immunoglobulin A; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D015848:Interleukin-5; D018123:Receptors, Interleukin; D019009:Proto-Oncogene Proteins c-kit