Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase.
We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA.
Iwakiri
Ryo
R
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Yoshihira
Kunichika
K
Ngadiman
Futagami
Taiki
T
Goto
Masatoshi
M
Furukawa
Kensuke
K
D000421:Alcaligenes / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019475:Camphor 5-Monooxygenase / Q000235:genetics*; D049308:Dioxygenases / Q000235:genetics*; D004980:Ethane / Q000031:analogs & derivatives* / Q000378:metabolism*; D005818:Genetic Engineering; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*
D006843:Hydrocarbons, Chlorinated; D049308:Dioxygenases; D019475:Camphor 5-Monooxygenase; D004980:Ethane; C016036:pentachloroethane