[Activation in the metaloproteinases complex in the follicle-embryonic generative capability].
OBJECTIVE
To evaluate the impact of the enzymatic inhibition complex of metaloproteinases [TIMP(MMP-3 stromelisine-1)] in follicle/genesis processes and their ovule/embryonic subsequent development in stimulated cycles.


METHODS
Prospective longitudinal in research center.


METHODS
A total of 20 patients were evaluated in vitro fertilization cycles measuring matrix metaloproteinases concentrations in days 3 and 12 of ovarian stimulation, as well as in the follicular liquid at the moment of ovarian retrieval. The determination of [TIMP/(MMP-3 stromelisine-1)] was done by ELISA and monoclonal antibodies type of immunoassay methods. For its statistical evaluation were applied linear regression models ( r/r2 ) and Kolmogorov-Smirov test comparing the two groups where values were expressed in agreement at its mean, standard deviation and a significance of p < 0.05.


RESULTS
For regression models was found a positive correlation between basal FSH and the age of patients [(r2 = 0.26)(p = 0.003)], dividing groups in older and younger patients than 35 years; it was observed significant difference in metaloproteinases concentration as in serum concentration (days 3 and 12 of stimulation) as in the follicular liquid. A decrease in the 14% [(TIMP/(MMP-3/stromelisine-1)] complex concentration was increasing in accordance at the female patients age.


CONCLUSIONS
The matrix metaloproteinases complex study has allowed observing a status not only in the ovular quality but in the embryonic development and fertilization processes too.
Barroso
Gerardo
G
Unidad de Reproducción Asistida, Departamento de Ginecología y Obstetricia, Clínica Lomas Altas, México, DF.
Corona de Lau
Clara
C
González
Julio
J
Loera
Lorena
L
Aguilar
Belén
B
Rosado
Yadira
Y
Reyes
Humberto
H
D000328:Adult; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D045726:Metalloproteases / Q000502:physiology*; D006080:Ovarian Follicle / Q000201:enzymology*; D011446:Prospective Studies
D045726:Metalloproteases