Developmental changes of Ni(2+) sensitivity and automaticity in Nkx2.5-positive cardiac precursor cells from murine embryonic stem cell.
BACKGROUND
It is controversial which subtypes of T type Ca(2+) channels are implicated in automaticity of cardiac cells during the embryonic period.


RESULTS
The effect of Ni(2+) on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40 micromol/L Ni(2+), which is enough to block Ni(2+)sensitive T type-Ca(2+) channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni(2+) did not show any effects on the automaticity of 50% of the cells in the late stage.


CONCLUSIONS
These results indicate that Ni(2+)-sensitive T-type Ca(2+) channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.
Manabe
Kasumi
K
Department of Genetic Medicine and Regenerative Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Tottori, Japan.
Miake
Junichiro
J
Sasaki
Norihito
N
Furuichi
Hitomi
H
Yano
Shuichi
S
Mizuta
Einosuke
E
Yamamoto
Yasutaka
Y
Hoshikawa
Yoshiko
Y
Yamazaki
Hidetoshi
H
Tajima
Fumihito
F
Shiota
Goshi
G
Nanba
Eiji
E
Ohgi
Shigetsugu
S
Hidaka
Kyoko
K
Morisaki
Takayuki
T
Kurata
Yasutaka
Y
Lee
Jong-Kook
JK
Igawa
Osamu
O
Shigemasa
Chiaki
C
Hisatome
Ichiro
I
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D047108:Embryonic Development / Q000502:physiology*; D006321:Heart / Q000196:embryology*; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000502:physiology*; D051379:Mice; D009532:Nickel / Q000494:pharmacology*; D011498:Protein Precursors / Q000187:drug effects / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000502:physiology*
D015220:Calcium Channels; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C484469:Nkx2-5 protein, mouse; D011498:Protein Precursors; D014157:Transcription Factors; D009532:Nickel