title: The 90K protein increases major histocompatibility complex class I expression and is regulated by hormones, gamma-interferon, and double-strand polynucleotides.
abstract: Here we report the cloning of the rat 90K, a homolog of the mouse cyclophilin C-associated protein/mouse adherent macrophage and human 90K. The protein is constitutively expressed by FRTL-5 thyrocytes, and its levels are modulated by TSH, insulin/IGF-I, and gamma-interferon. Transfection of the cells with 90K cDNA or exposure to purified 90K resulted in a significant increase of the expression of major histocompatibility complex class I but not class II antigens. An increased expression of 90K was obtained after viral infection or introduction into the cells of fragments of viral, bacterial, or mammalian double-strand polynucleotides. The increase was sequence independent, not CpG mediated, and associated with the expression of molecules characterizing antigen-presenting-cell phenotype. The present data along with results from previous studies suggest that 90K plays an important role in the maintenance of an appropriate level of immune response.
authors.affiliation: Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Antonino
authors.initials: A
authors.lastname: Grassadonia
authors.forename: Nicola
authors.initials: N
authors.lastname: Tinari
authors.forename: Bruno
authors.initials: B
authors.lastname: Fiorentino
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakazato
authors.forename: Michele
authors.initials: M
authors.lastname: De Tursi
authors.forename: Cesidio
authors.initials: C
authors.lastname: Giuliani
authors.forename: Giorgio
authors.initials: G
authors.lastname: Napolitano
authors.forename: Dinah S
authors.initials: DS
authors.lastname: Singer
authors.forename: Stefano
authors.initials: S
authors.lastname: Iacobelli
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Kohn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins; D006561:Herpes Simplex / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011119:Polynucleotides / Q000235:genetics / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D015395:Histocompatibility Antigens Class I; D006728:Hormones; C492608:Lgals3bp protein, rat; D011119:Polynucleotides; D011506:Proteins; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA