title: Partial purification and biochemical characterization of a soluble alpha-glucosidase II-like activity from Candida albicans.
abstract: A soluble alpha-glucosidase was partially purified from Candida albicans cells by a three-step procedure consisting of size-exclusion, ion-exchange and adsorption chromatographies. After the last step, enzyme was enriched about 8.7-fold with a yield of 13% over the starting material and analysis of the purified preparation revealed two major polypeptides of 36 and 47 kDa. The latter was responsible for enzyme activity as visualized with a fluorescent substrate. Nigerose, an alpha-1,3-linked glucose disaccharide, was preferentially hydrolyzed by the purified enzyme over other glucosedisaccharides bearing distinct alpha-linkages. The purified alpha-glucosidase also converted the GlcMan9GlcNAc2 oligosaccharide into the Man9GlcNAc2 product in a time-dependent manner. These and other determined properties are consistent with a type GII alpha-glucosidase probably involved in N-glycan processing.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad Autonoma de Guanajuato, Guanajuato, Guanajuato CP 36000, México.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Torre-Bouscoulet
authors.forename: Everardo
authors.initials: E
authors.lastname: López-Romero
authors.forename: Rosalía
authors.initials: R
authors.lastname: Balcázar-Orozco
authors.forename: Carlos
authors.initials: C
authors.lastname: Calvo-Méndez
authors.forename: Arturo
authors.initials: A
authors.lastname: Flores-Carreón
mesh_terms: D002176:Candida albicans / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D011134:Polysaccharides / Q000378:metabolism; D012995:Solubility; D000520:alpha-Glucosidases / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D000520:alpha-Glucosidases