Analysis of bioactive ingredients in the brown alga Fucus vesiculosus by capillary electrophoresis and neutron activation analysis.
Two different types of bioactive components of the seaweed Fucus vesiculosus were analysed: (1) polyphenols (phlorotannins) by capillary electrophoresis (CE) and (2) mineral part (including bioactive microelements) by neutron activation analysis (NAA). CE experiments were carried out using a UV detector (at 210 nm) and an uncoated silica capillary. The best separation was achieved at a voltage of 20 kV using borate or acetate buffer in a methanol/acetonitrile mixture as background electrolyte. The CE analysis data were confirmed by high-performance liquid chromatography (HPLC). Determination of mineral composition of algal biomass by NAA was performed on the basis of various nuclides; the best results (from 38 elements determined) were obtained for Mn, Fe, Zn, As, Br, Sr, I, Ba, Au and Hg.
Truus
Kalle
K
Department of Natural Sciences, Tallinn Pedagogical University, Narva mnt 25, 10120 Tallinn, Estonia.
Vaher
Merike
M
Koel
Mihkel
M
Mähar
Andres
A
Taure
Imants
I
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D044645:Fucus / Q000737:chemistry*; D008903:Minerals / Q000032:analysis; D009501:Neutron Activation Analysis / Q000379:methods*; D010636:Phenols / Q000032:analysis
D008903:Minerals; D010636:Phenols