title: Comparison of digestive microflora between feedlot cattle with and without infection by Cryptosporidium andersoni.
abstract: The influence of cryptosporidial abomasitis on digestive anaerobic microflora in feedlot cattle with spontaneous Cryptosporidium andersoni colonization of abomasum was shown. Significant differences were found after the cultivation of abomasal content. Scopulariopsis brevicaulis was detected in damaged areas of infected abomasum. PCR analysis of ruminal fluid showed no differences between the two animal groups regarding qualitative composition of anaerobic and facultative anaerobic rumen microflora. The concentration of volatile fatty acids (acetate, propionate, butyrate) and ammonia in the rumen content showed that examined metabolic parameters were within normal limits except for ammonia content, which was higher in infected (291 mg/kg) than in healthy animals (203 mg/kg).
authors.affiliation: State Veterinary Institute, 165 03 Prague, Czechia. ivan.holko@svupraha.cz
authors.forename: I
authors.initials: I
authors.lastname: Holko
authors.forename: I
authors.initials: I
authors.lastname: Pavlásek
authors.forename: L
authors.initials: L
authors.lastname: Barton
authors.forename: V
authors.initials: V
authors.lastname: Kmet
mesh_terms: D000018:Abomasum / Q000382:microbiology* / Q000473:pathology; D000641:Ammonia / Q000032:analysis; D000818:Animals; D001203:Ascomycota / Q000302:isolation & purification / Q000472:pathogenicity; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003457:Cryptosporidiosis / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005232:Fatty Acids, Volatile / Q000032:analysis; D005658:Fungi / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012417:Rumen / Q000737:chemistry / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005232:Fatty Acids, Volatile; D000641:Ammonia