Genotypic analysis of sequential genital herpes simplex virus type 1 (HSV-1) isolates of patients with recurrent HSV-1 associated genital herpes.
Clinical recurrences of Herpes simplex virus type 1 (HSV-1)-associated genital herpes are thought to be caused by reactivation of latent endogenous HSV-1. However, the possibility of reinfection with exogenous HSV-1 cannot be excluded. This study aimed to determine the incidence of genital HSV-1 superinfection in patients by investigating the genotype of sequential HSV-1 isolates obtained from the same anatomical site of patients with clinical recurrences of genital HSV-1 recurrent genital herpes. Sequential genital HSV-1 isolates were genotyped by PCR amplification of the hypervariable regions located within the HSV-1 genes US1 and US12. Whereas the sequential HSV-1 isolates in 11 of the 13 patients studied had the same genotypes, the sequential isolates of 2 patients showed a different genotype. The data suggest that HSV-1-induced recurrent genital herpes can be associated with genital reinfection with an exogenous HSV-1 strain.
Roest
R W
RW
Departments of Dermatology and Venereology, Glasgow, Scotland.
Carman
W F
WF
Maertzdorf
J
J
Scoular
A
A
Harvey
J
J
Kant
M
M
Van Der Meijden
W I
WI
Verjans
Georges M G M
GM
Osterhaus
A D M E
AD
D000328:Adult; D005260:Female; D005838:Genotype; D006558:Herpes Genitalis / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012008:Recurrence; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
C105843:ICP47 protein, Herpes simplex virus; D017874:Immediate-Early Proteins; D014764:Viral Proteins