title: Development of an in vitro, isolated, infected spore testing model for disinfectant testing of Microsporum canis isolates.
abstract: The isolated infected hair model is a commonly used technique to test the fungicidal efficacy of topical therapies against Microsporum canis. The most commonly used model uses mats of infective hairs, and results from various laboratories have differed. The objectives of this study were to develop a method to produce spores for testing when only mycelial forms were available and to develop a semiquantitative testing method that used only infective spores from hairs, and not pooled hair samples for testing. Ten isolates of M. canis were used in this study. Juvenile guinea pigs were easily infected using mycelial forms of M. canis and large numbers of spores were easily harvested for testing. Eight dilutions of disinfectants were tested. Fungal culture data were evaluated using an endpoint dilution at which there was 100% fungicidal activity, i.e. no growth on the plates. The 10 samples showed identical results. Chlorhexidine and Virkon(R) S were ineffective even when used at x4 the manufacturer's recommended dilution. Lime sulphur (1 : 33), enilconazole (20 microL mL(-1)), and bleach (1 : 10) were consistently effective when used at the recommended dilution. In addition, lime sulphur and enilconazole were 100% fungicidal even when the recommended concentration was diluted 1 : 4 or x4 as dilute as recommended.
authors.affiliation: Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive West, Madison, WI 53706, USA. moriellk@svm.vetmed.wisc.edu
authors.forename: Karen A
authors.initials: KA
authors.lastname: Moriello
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Deboer
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Volk
authors.forename: Andrew
authors.initials: A
authors.lastname: Sparkes
authors.forename: Ann
authors.initials: A
authors.lastname: Robinson
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002371:Cat Diseases / Q000382:microbiology* / Q000517:prevention & control*; D002415:Cats; D003881:Dermatomycoses / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004195:Disease Models, Animal; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006168:Guinea Pigs; D006197:Hair / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects* / Q000302:isolation & purification; D010865:Pilot Projects; D013172:Spores, Fungal / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D004202:Disinfectants