title: A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes.
abstract: A large-scale mutagenesis screen was performed in Medaka to identify genes acting in diverse developmental processes. Mutations were identified in homozygous F3 progeny derived from ENU-treated founder males. In addition to the morphological inspection of live embryos, other approaches were used to detect abnormalities in organogenesis and in specific cellular processes, including germ cell migration, nerve tract formation, sensory organ differentiation and DNA repair. Among 2031 embryonic lethal mutations identified, 312 causing defects in organogenesis were selected for further analyses. From these, 126 mutations were characterized genetically and assigned to 105 genes. The similarity of the development of Medaka and zebrafish facilitated the comparison of mutant phenotypes, which indicated that many mutations in Medaka cause unique phenotypes so far unrecorded in zebrafish. Even when mutations of the two fish species cause a similar phenotype such as one-eyed-pinhead or parachute, more genes were found in Medaka than in zebrafish that produced the same phenotype when mutated. These observations suggest that many Medaka mutants represent new genes and, therefore, are important complements to the collection of zebrafish mutants that have proven so valuable for exploring genomic function in development.
authors.affiliation: Japan Science and Technology Corporation, Kondoh Differentiation Signaling Project, Kawaaracho 14, Yoshida, Sakyoku, Kyoto 606-8305, Japan. furutaniseiki@dsp.jst.go.jp
authors.forename: Makoto
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authors.lastname: Furutani-Seiki
authors.forename: Takao
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authors.lastname: Sasado
authors.forename: Chikako
authors.initials: C
authors.lastname: Morinaga
authors.forename: Hiroshi
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authors.lastname: Suwa
authors.forename: Katsutoshi
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authors.lastname: Niwa
authors.forename: Hiroki
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authors.lastname: Yoda
authors.forename: Tomonori
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authors.lastname: Deguchi
authors.forename: Yukihiro
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authors.lastname: Hirose
authors.forename: Akihito
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authors.lastname: Yasuoka
authors.forename: Thorsten
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authors.lastname: Henrich
authors.forename: Tomomi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Norimasa
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authors.lastname: Iwanami
authors.forename: Daiju
authors.initials: D
authors.lastname: Kitagawa
authors.forename: Kota
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authors.lastname: Saito
authors.forename: Satoshi
authors.initials: S
authors.lastname: Asaka
authors.forename: Masakazu
authors.initials: M
authors.lastname: Osakada
authors.forename: Sanae
authors.initials: S
authors.lastname: Kunimatsu
authors.forename: Akihiro
authors.initials: A
authors.lastname: Momoi
authors.forename: Harun
authors.initials: H
authors.lastname: Elmasri
authors.forename: Christoph
authors.initials: C
authors.lastname: Winkler
authors.forename: Mirana
authors.initials: M
authors.lastname: Ramialison
authors.forename: Felix
authors.initials: F
authors.lastname: Loosli
authors.forename: Rebecca
authors.initials: R
authors.lastname: Quiring
authors.forename: Matthias
authors.initials: M
authors.lastname: Carl
authors.forename: Clemens
authors.initials: C
authors.lastname: Grabher
authors.forename: Sylke
authors.initials: S
authors.lastname: Winkler
authors.forename: Filippo
authors.initials: F
authors.lastname: Del Bene
authors.forename: Ai
authors.initials: A
authors.lastname: Shinomiya
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kota
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Takahashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Todo
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takahama
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Katada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishina
authors.forename: Noboru
authors.initials: N
authors.lastname: Nakajima
authors.forename: Joachim
authors.initials: J
authors.lastname: Wittbrodt
authors.forename: Hisato
authors.initials: H
authors.lastname: Kondoh
mesh_terms: D000818:Animals; D005123:Eye / Q000196:embryology; D005854:Germ Cells; D009154:Mutation; D038081:Organogenesis / Q000235:genetics*; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D010641:Phenotype; D016548:Prosencephalon / Q000196:embryology; D011836:Radiation Tolerance / Q000235:genetics; D012107:Research Design; D019170:Somites; D013950:Thymus Gland / Q000196:embryology