Identification of beta-tubulin isoform V as an autoantigen in allergic rhinitis by a proteomic approach.
Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively, using proteomics. Specifically, we separated proteins extracted from peripheral blood mononuclear cells by 2-dimensional electrophoresis and then detected autoantigens by subsequent western blotting with sera from patients with allergic rhinitis. As a result, we detected multiple autoantigens, some of which were further identified by mass fingerprinting. Next, we confirmed antigenicity of one of the identified autoantigens, beta-tubulin isoform V (beta-tubV), using a recombinant protein and then measured prevalence of the anti-beta-tubV autoantibodies. As a result, 52% of the tested patients with allergic rhinitis were found to possess anti-beta-tubV autoantibodies. Our study indicates that autoimmunity is a common phenomena and beta-tubV is one of the major autoantigens in allergic rhinitis.
Nakamura
Manabu
M
Department of Bioregulation, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
Tsutsumi
Kouichiro
K
Ooka
Seido
S
Sekine
Taichi
T
Koizuka
Izumi
I
Nishioka
Kusuki
K
Kato
Tomohiro
T
D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D020033:Protein Isoforms / Q000276:immunology / Q000302:isolation & purification; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification; D040901:Proteomics; D018343:Receptors, Adrenergic, beta-2 / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D014404:Tubulin / Q000276:immunology* / Q000302:isolation & purification
D001323:Autoantibodies; D001324:Autoantigens; D020033:Protein Isoforms; D011506:Proteins; D018343:Receptors, Adrenergic, beta-2; D014404:Tubulin; D007073:Immunoglobulin E