Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1.
Aneuploidy, resulting from chromosome missegregation during meiosis, is a major cause of human infertility and birth defects. However, its molecular basis remains incompletely understood. Here we have identified a spectrum of chromosome anomalies in embryos of zebrafish homozygous for a hypomorphic mutation in Mps1, a kinase required for the mitotic checkpoint. These aneuploidies are caused by meiotic error and result in severe developmental defects. Our results reveal Mps1 as a critical regulator of chromosome number in zebrafish, and demonstrate how slight genetic perturbation of a mitotic checkpoint factor can dramatically reduce the fidelity of chromosome segregation during vertebrate meiosis.
Poss
Kenneth D
KD
Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA. k.poss@cellbio.duke.edu
Nechiporuk
Alex
A
Stringer
Keith F
KF
Lee
Charles
C
Keating
Mark T
MT
D000782:Aneuploidy; D000818:Animals; D018797:Cell Cycle Proteins; D002869:Chromosome Aberrations; D004625:Embryo, Nonmammalian / Q000473:pathology; D005260:Female; D005313:Fetal Death / Q000235:genetics; D008297:Male; D008938:Mitosis; D009154:Mutation; D009865:Oocytes / Q000502:physiology*; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000502:physiology; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D018797:Cell Cycle Proteins; D029961:Zebrafish Proteins; D011494:Protein Kinases; C490545:ttk protein, zebrafish; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; C075840:TTK protein, human