A new module for quantitative evaluation of repellent efficacy using human subjects.
A new module for quantitative evaluation of arthropod repellents in human subjects was designed, constructed, and protocols for use of the module were developed. Doses of 3 arthropod repellents, 1-[3-cyclohexen-1-ylcarbonyl] piperidine (AI3-35765), 1-[3-cyclohexen-1-ylcarbonyl]-2-methylpiperidine (AI3-37220), and N,N-diethyl-3-methylbenzamide (deet), were evaluated using the mosquito, Anopheles stephensi Liston. Biting responses to varied doses of the repellents on human skin were quantified, and the effectiveness of the 3 repellents was compared. The new module consists of 6 test cells and permits the simultaneous comparison of up to 5 repellent doses or chemical types and a control using a complete randomized block design with minimal treatment interaction and with > or = 6 replicates per human subject.
Klun
J A
JA
Insect Chemical Ecology Laboratory, USDA-ARS, Beltsville, MD 20705, USA.
Debboun
M
M
D000818:Animals; D003671:DEET / Q000633:toxicity; D005260:Female; D006801:Humans; D007302:Insect Repellents / Q000494:pharmacology*; D010880:Piperidines / Q000633:toxicity*; D015203:Reproducibility of Results
D007302:Insect Repellents; D010880:Piperidines; D003671:DEET; C075892:1-(3-cyclohexen-1-ylcarbonyl)-2-methylpiperidine