title: Serological characterization and prevalence of spvR genes in Salmonella isolated from foods involved in outbreaks in Brazil.
abstract: Salmonella strains (n = 75) isolated from foods involved in foodborne outbreaks occurred in Rio Grande do Sul State, Brazil, during 1999 and 2000 were studied. Strains were serotyped and submitted to PCR analysis to verify the prevalence of Salmonella plasmid virulence (spvR) regulatory gene. Among the 75 isolates, 73 (97%) were classified as Salmonella enterica serovar Enteritidis. All of the Salmonella strains isolated in 1999 were classified as serotype Enteritidis, whereas in 2000 two isolates were serotyped as Salmonella Derby and Salmonella Typhimurium. Regarding the prevalence of spvR gene, 62 strains (82.7%) were PCR positive, and a positive correlation (P < 0.05) between the strains of Salmonella Enteritidis and the presence of spvR gene was demonstrated, which suggests that this gene is a characteristic of the Salmonella Enteritidis analyzed.
authors.affiliation: Faculdade de Biociências, PUCRS, Av. Ipiranga, 6681, prédio 12A, Cep 90619-900, Porto Alegre, Brazil.
authors.forename: Mercedes P
authors.initials: MP
authors.lastname: Geimba
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Tondo
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: de Oliveira
authors.forename: Cláudio W
authors.initials: CW
authors.lastname: Canal
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
mesh_terms: D001938:Brazil / Q000453:epidemiology; D004196:Disease Outbreaks; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping; D014774:Virulence / Q000235:genetics