title: Working without accumulation membrane in flow field-flow fractionation. Effect of sample loading on retention.
abstract: Membraneless hyperlayer flow field-flow fractionation (Hyp FIFFF) has shown improved performance with respect to Hyp FIFFF with membrane. The conditions for high recovery and recovery independent of sample loading in membraneless Hyp FIFFF have been previously determined. The effect of sample loading should be also investigated in order to optimize the form of the peaks for real samples. The effect of sample loading on peak retention parameters is of prime importance in applications such as the conversion of peaks into particle size distributions. In this paper, a systematic experimental work is performed in order to study the effect of sample loading on retention parameters. A procedure to regenerate the frit operating as accumulation wall is described. High reproducibility is obtained with low system conditioning time.
authors.affiliation: Department of Chemistry "G. Ciamician", University of Bologna, Via F. Selmi 2, 1-40126 Bologna, Italy. melucci@ciam.unibo.it
authors.forename: Dora
authors.initials: D
authors.lastname: Melucci
authors.forename: Andrea
authors.initials: A
authors.lastname: Zattoni
authors.forename: Sonia
authors.initials: S
authors.lastname: Casolari
authors.forename: Matteo
authors.initials: M
authors.lastname: Reggiani
authors.forename: Ramses
authors.initials: R
authors.lastname: Sanz
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Reschiglian
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Torsi
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005374:Filtration; D010316:Particle Size; D015203:Reproducibility of Results; D013048:Specimen Handling