title: [Establishment of two-dimensional gel electrophoresis technical platform for proteome research].
abstract: OBJECTIVE
  To establish and optimize two-dimensional gel electrophoresis technique for proteomics.
  
  
  METHODS
  Immobiline pH gradients isoelectric focusing was used as 1D, vertical SDS-PAGE as 2D, some factors such as method and volume of loading sample, choice of gels, concentration of SDS gels, preset of electric program and parameters, protocol for staining the gels were improved.
  
  
  CONCLUSIONS
  Using the proper method described above, satisfactory 2DE maps of human embryonic lung fibroblast(HLF) and silkworm egg were obtained. 2DE technical platform for proteome research was set up.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.forename: Shisong
authors.initials: S
authors.lastname: Fang
authors.forename: Xiyi
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005333:Fetus; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology; D016678:Genome; D006801:Humans; D008168:Lung / Q000166:cytology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome