The importance of virus-associated host ICAM-1 in human immunodeficiency virus type 1 dissemination depends on the cellular context.
The primary objective of this study was to define whether the nature of virion-bound host cell membrane proteins influenced the process of human immunodeficiency virus 1 (HIV-1) capture and transmission. We pulsed cells of monocytoid lineage (established and primary) and CD4-negative epithelial cells transiently expressing DC-SIGN or LFA-1 with isogenic HIV-1 particles either devoid or bearing host-derived ICAM-1 or ICAM-3 before incubation with an indicator cell line. To our surprise, the ICAM-1/LFA-1 association was a more efficient transmission factor than the combined gp120/DC-SIGN and ICAM-3/DC-SIGN interactions. The involvement of the association between virus-bound ICAM-1 and its natural ligand LFA-1 in virus binding and carriage was confirmed when using more physiological cellular targets, i.e., human lymphoid tissues cultured ex vivo. However, the contribution of virus-anchored host ICAM-1 to the process of retention and transmission of HIV-1 could not be confirmed when using primary human cells of macrophage/dendritic lineage as transmitter cells and autologous CD4+ T lymphocytes as targets. Altogether these data underscore the complexity of factors participating in virus-cell contact and efficient dissemination of HIV-1 to target cells.
Bounou
Salim
S
Research Center in Infectious Diseases, CHUL Research Center, and Faculty of Medicine, Laval University, Quebec, Canada.
Giguere
Jean-François
JF
Cantin
Réjean
R
Gilbert
Caroline
C
Imbeault
Michael
M
Martin
Geneviève
G
Tremblay
Michel J
MJ
D015703:Antigens, CD / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured / Q000821:virology; D003713:Dendritic Cells / Q000821:virology; D004847:Epithelial Cells / Q000821:virology; D017930:Genes, Reporter; D015497:HIV-1 / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D037181:Lectins, C-Type / Q000235:genetics / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000821:virology*; D008264:Macrophages / Q000821:virology; D009000:Monocytes / Q000821:virology; D009924:Organ Culture Techniques; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D014162:Transfection; D014771:Virion / Q000737:chemistry; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1; D037181:Lectins, C-Type; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C483616:ICAM3 protein, human