Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation.
A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.
Sato
Kae
K
Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama 351-0198, Japan.
Sawayanagi
Munetaka
M
Hosokawa
Kazuo
K
Maeda
Mizuo
M
D001360:Avidin / Q000737:chemistry*; D009154:Mutation; D036103:Nanotechnology; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*
D011137:Polystyrenes; C093483:neutravidin; D001360:Avidin