Polymerase chain reaction in detection of human granulocytic ehrlichiosis (HGE) agent DNA in Ixodes ricinus ticks.
PCR, or polymerase chain reaction, is the most hoped-for diagnostic method for ehrlichiosis detection. In European molecular laboratories, the gene encoding 16S rRNA for ribosomal small subunit has been the most frequently used Ehrlichia DNA marker. Due to a lack of PCR standardization, this work compares sensitivity of two pairs of primers that amplify the 16S rDNA gene fragments.
Skotarczak
Bogumiła
B
Katedra Genetyki Uniwersytetu Szczecińskiego al. Plastów 40 B, 71-065 Szczecin. bogumila_skotarczak@univ.szczecin.pl
Rymaszewska
Anna
A
Adamska
Małgorzata
M
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D006098:Granulocytes / Q000382:microbiology*; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S