Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukaemia.
EEN (extra eleven nineteen), also known as EA2 (endophilin A2), a fusion partner of the MLL (mixed-lineage leukaemia) gene in human acute leukaemia, is a member of the endophilin A family, involved in the formation of endocytic vesicles. We present evidence to show that EEN/EA2 is localized predominantly in nuclei of various cell lines of haemopoietic, fibroblast and epithelial origin, in contrast with its reported cytoplasmic localization in neurons and osteoclasts, and that EEN/EA2 exhibits nucleocytoplasmic shuttling. During the cell cycle, EEN/EA2 shows dynamic localization: it is perichromosomal in prometaphase, co-localizes with the bipolar spindle in metaphase and anaphase and redistributes to the midzone and midbody in telophase. This pattern of distribution coincides with changes in protein levels of EEN/EA2, with the highest levels being observed in G2/M-phase. Our results suggest that distinct subcellular localization of the endophilin A family members probably underpins their diverse cellular functions and indicates a role for EEN/EA2 in the cell cycle.
Cheung
Ngai
N
Department of Pathology, University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China.
So
Chi Wai
CW
Yam
Judy W P
JW
So
C K C
CK
Poon
Randy Y C
RY
Jin
Dong-Yan
DY
Chan
Li Chong
LC
D000818:Animals; D000918:Antibody Specificity; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007938:Leukemia / Q000378:metabolism; D008938:Mitosis / Q000502:physiology; D021381:Protein Transport; D011506:Proteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D017384:Sequence Deletion; D013347:Subcellular Fractions / Q000378:metabolism*; D018909:src Homology Domains
D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C105159:SH3GL1 protein, human