Vitamin D receptor (VDR) promoter targeting through a novel chromatin remodeling complex.
We have purified nuclear complexes for Vitamin D receptor (VDR), and identified one of them as a novel ATP-dependent chromatine remodeling containing Williams syndrome transcription factor (WSTF), that is supposed to be responsible for Williams syndrome. This complex (WSTF including nucleosome assembly complex (WINAC)) exhibited an ATP-dependent chromatin remodeling activity in vitro. Transient expression assays revealed that WINAC potentiates ligand-induced function of VDR in gene activation and repression. Thus, this study describes a molecular basis of the VDR function on chromosomal DNA through chromatine remodeling.
Kato
Shigeaki
S
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. uskato@mail.ecc.u-tokyo.ac.jp
Fujiki
Ryoji
R
Kitagawa
Hirochika
H
D002843:Chromatin / Q000737:chemistry*; D008024:Ligands; D011401:Promoter Regions, Genetic; D018167:Receptors, Calcitriol / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry
D002843:Chromatin; D008024:Ligands; D018167:Receptors, Calcitriol; D014157:Transcription Factors