[Impact of dust storm PM2.5 and PM10 on the phagocytic function of alveolar macrophages of rat].
OBJECTIVE
To investigated effects of dust storm PM2.5 and PM10 on the phagocytosis of rat alveolar macrophages.


METHODS
PM2.5 and PM10 were collected during dust storm period in the urban area of Beijing. Cytotoxicity of PM2.5 and PM10 was measured by MTT method. Flow cytometry analysis was applied to characterize effects of PM2.5 and PM10 on the phagocytosis of alveolar macrophages.


RESULTS
PM2.5 and PM10 were toxic to rat alveolar macrophages at the concentrations above 20 micrograms/ml. PM2.5 and PM10 impaired phagocytic function of alveolar macrophages in a dose-dependent manner. PM2.5 was more potent than PM10.


CONCLUSIONS
Dust storm PM2.5 and PM10 could impair the phagocytosis of rat alveolar macrophages, that may in turn impair the airway nonspecific defenses function.
Huang
Xnelian
X
School of Public Health, Peking University, Beijing 100083, China.
Jin
Yu
Y
Guo
Xinbiao
X
Wang
Yun
Y
D000392:Air Movements; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D003889:Desert Climate; D004391:Dust; D004784:Environmental Monitoring; D016676:Macrophages, Alveolar / Q000276:immunology*; D010316:Particle Size; D010587:Phagocytosis / Q000276:immunology*; D051381:Rats; D012621:Seasons
D000393:Air Pollutants; D004391:Dust