title: Phospholipase A2 mRNA expression in the nasal mucosa of healthy subjects and patients with seasonal allergic rhinitis.
abstract: Phospholipase A2 (PLA2) is a family of enzymes that play different role(s) in inflammation, but their importance in seasonal allergic rhinitis (SAR) has not been clarified. Here, we determined the levels of messenger ribonucleic acid (mRNA) for different PLA2 types in the nasal mucosa of SAR patients (n = 6) and healthy controls (n = 5). Nasal brush samples were taken both during pollen season, when the symptoms of the patients were severe, and off-season, when the patients were free of symptoms. We found that PLA2 IB, IIA, IID, IIE, IIF, III, IVA, IVB, IVC, VIA, VIB, VIIA, VIIB, VIIIA, VIIIB, X, XII and XIII were all expressed in each subject at both occasions. The mRNA levels of PLA2 VIIA (platelet-activating factor (PAF) acetylhydrolase) were lower in SAR patients than controls, both during pollen season (p = 0.03) and off season (p = 0.03). These findings demonstrate that a large number of PLA2 types are expressed in the nasal mucosa, regardless of whether there is ongoing allergic inflammation or not. The observation that PAF acetylhydrolase mRNA expression in the nasal mucosa is lower in SAR patients than in healthy subjects suggests the possibility that impaired ability to inactivate PAF might be of importance in SAR. Further studies are required to clarify whether the decreased PAF acetylhydrolase mRNA expression in SAR is accompanied by decreased enzyme activity and whether aberrations in PAF acetylhydrolase are present in infectious rhinitis patients as well.
authors.affiliation: Department of Molecular and Clinical Medicine, Division of Occupational and Environmental Medicine, University of Linkôping, S-581 85 Linkôping, Sweden. Johli@imk.liu.se
authors.forename: John
authors.initials: J
authors.lastname: Lindbom
authors.forename: Anders G
authors.initials: AG
authors.lastname: Ljungman
authors.forename: Kristina
authors.initials: K
authors.lastname: Irander
authors.forename: Mats
authors.initials: M
authors.lastname: Lindahl
authors.forename: Christer
authors.initials: C
authors.lastname: Tagesson
mesh_terms: D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000235:genetics; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism*; D012621:Seasons; D014598:Uteroglobin / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C556389:SCGB1A1 protein, human; D014598:Uteroglobin; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D010741:Phospholipases A; D054467:Phospholipases A2