The epitaxial growth of cholesterol crystals from bile solutions on calcite substrates.
Epitaxial relationships between the surfaces of inorganic and bioorganic crystals can be an important factor in crystal nucleation and growth processes in a variety of biological environments. Crystalline cholesterol monohydrate (ChM), a constituent of both gallstone and atherosclerotic plaques, is often found in association with assorted mineral phases. Using in situ atomic force microscopy (AFM) and well-characterized model bile solutions, the nucleation and epitaxial growth of ChM on calcite (104) surfaces in real-time is demonstrated. The growth rates of individual cholesterol islands formed on calcite substrates were determined at physiological temperatures. Evidence of Ostwald's ripening was also observed under these experimental conditions. The energetics of various (104) calcite/(001) ChM interfaces were calculated to determine the most stable interfacial structure. These simulations suggest that the interface is fully hydrated and that cholesterol hydroxyl groups are preferentially positioned above carbonate ions in the calcite surface. This combination of experimental and theoretical work provides a clearer picture of how preexisting mineral seeds might provide a viable growth template that can reduce the energetic barrier to cholesterol nucleation under some physiological conditions.
Frincu
M Crina
MC
Department of Chemistry, Georgetown University, 37th and O Streets NW, Washington, DC 20057-1227, USA.
Fleming
Sean D
SD
Rohl
Andrew L
AL
Swift
Jennifer A
JA
D001161:Arteriosclerosis / Q000139:chemically induced; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D002119:Calcium Carbonate / Q000737:chemistry* / Q000378:metabolism; D002769:Cholelithiasis / Q000209:etiology; D002784:Cholesterol / Q000737:chemistry*; D003460:Crystallization; D005704:Gallbladder / Q000737:chemistry; D042882:Gallstones / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D008903:Minerals / Q000737:chemistry; D012996:Solutions; D013696:Temperature; D013997:Time Factors
D008903:Minerals; D012996:Solutions; D002784:Cholesterol; D002119:Calcium Carbonate