[Screening and characterization of dominant bacteria for the degradation of endocrine disruptor di-n-butyl phthalate].
OBJECTIVE
In order to screen the dominant bacteria for degrading endocrine disruptor di-n-butyl phthalate (DBP).


METHODS
The activated sludge was acclimated by DBP as sole carbon source. DBP concentration was increased progressively. 5 strains were isolated from the sludge by the plane-table after 10 weeks, 1 strain of which grew and degraded DBP well.


RESULTS
By orthogonal test, it was indicated that the appropriate degradation conditions of DBP for this best strain were degradation time 32 h, DBP concentration 200 mg/L, rate of shaking incubator 100 r/min, pH 7.0, temperature 30 degrees C. The degraded rate of DBP is more than 95% under above conditions.


CONCLUSIONS
The bacterium was identified as Pseudomonas.
Wang
Lin
L
Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Luo
Qifang
Q
D001419:Bacteria / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D018505:Waste Management / Q000379:methods
D012722:Sewage; D003993:Dibutyl Phthalate