Possible involvement of AMP-activated protein kinase in obesity resistance induced by respiratory uncoupling in white fat.
The AMP-activated protein kinase (AMPK) cascade is a sensor of cellular energy charge that promotes catabolic and inhibits anabolic pathways. However, the role of AMPK in adipocytes is poorly understood. We show that transgenic expression of mitochondrial uncoupling protein 1 in white fat, which induces obesity resistance in mice, is associated with depression of cellular energy charge, activation of AMPK, downregulation of adipogenic genes, and increase in lipid oxidation. Activation of AMPK may explain the complex metabolic changes in adipose tissue of these animals and our results support a role for adipocyte AMPK in the regulation of storage of body fat.
Matejkova
Olga
O
Department of Adipose Tissue Biology and Center for Integrated Genomics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
Mustard
Kirsty J
KJ
Sponarova
Jana
J
Flachs
Pavel
P
Rossmeisl
Martin
M
Miksik
Ivan
I
Thomason-Hughes
Michaela
M
Grahame Hardie
D
D
Kopecky
Jan
J
D000263:Adenylate Kinase / Q000378:metabolism*; D000273:Adipose Tissue / Q000201:enzymology / Q000503:physiopathology*; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004822:Epididymis; D007113:Immunity, Innate / Q000235:genetics; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins; D009765:Obesity / Q000235:genetics*; D019301:Oleic Acid / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D019301:Oleic Acid; D000263:Adenylate Kinase