[Dynamic alterations of rat metabolic enzymes activities and liver damage in rat exposed to vinyl chloride monomer].
OBJECTIVE
Study the alterations of metabolic enzyme activities and liver functions induced by vinyl chloride monomer(VCM) and to explore the relationship between metabolic enzyme activities and liver functions.


METHODS
Animals were administered VCM for 12 weeks in doses of 0, 5, 10, 20 mg/kg (i.p.), and were victimized at week of 3rd, 6th, 9th, 12th. The activities of enzymes were determined using spectrophotography. The indexes of liver functions were assayed by automatic biochemistry analyzer. The histopathological alterations of rat livers were observed.


RESULTS
The activities of ALDH, CYP2E1, GSTs, were significantly different between groups, in addition, those differences can also be seen with the increase of experiment time. There was negative correlation between ALP and ADH (r = -0.649).


CONCLUSIONS
The degree of liver damage induced by VCM could be associated with the activities of liver metabolic enzymes. It was suggested that there was slight correlation between enzyme activities in serum and enzyme activities in liver.
Wang
Aihong
A
School of Public Health, Fudan University, Shanghai 200032, China.
Zhu
Shoumin
S
Zhou
Yuanling
Y
Sun
Yue
Y
D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014752:Vinyl Chloride / Q000633:toxicity*; D014753:Vinyl Compounds / Q000633:toxicity
D014753:Vinyl Compounds; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase; D014752:Vinyl Chloride