Haemolysins of Salmonella, their role in pathogenesis and subtyping of Salmonella serovars.
Haemolysin patterns of 175 strains of different Salmonella enterica subspecies enterica serovars isolated from different animal sources and places were determined using 11 different blood agar media made with either non-washed horse/sheep erythrocytes or with washed erythrocytes of cattle, sheep, horse, goat, rabbit, guinea pig, and human A, O and B blood groups. Study on 47 strains belonging to 10 serovars of Salmonella from buffalo meat (buffen), 42 strains of 11 serovars from goat meat (chevon): 16 strains of Salmonella enterica serovar Paratyphi B and 25 of S. enterica serovar Paratyphi B var Java from fish, meat, meat products and clinical cases; 45 isolates of S. Abortusequi from aborted mares (18), fetal contents (21), aborted donkey mares (2) and 4 reference strains, revealed that all host restricted Salmonella namely, S. enterica serovar Gallinarum, S. enterica serovar Anatum, S. enterica serovar Abortusequi and S. enterica serovar Paratyphi B could be divided into different haemolysin types based on their inability to produce haemolysis on one or more types of blood agar, while strains of all zoonotic Salmonella serovars induced haemolysis on all the 9 types of blood agar made of washed erythrocytes. None of 175 Salmonella could produce hemolytic colonies on blood agar made of non-washed horse/ sheep erythrocytes. Haemolysin type I (lysing all types of washed erythrocytes) was the commonest one among all serovars except S. Abortusequi, none of which lysed horse erythrocytes. Salmonella enterica serovar Abortusequi having hemolytic activity against sheep erythrocytes were more invasive but had lesser ability to survive in sheep mononuclear cells than non-hemolytic strains. Multiplicity of haemolysins appeared significant epidemiological tool.
Singh
B R
BR
National Salmonella Centre (Vet.), Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar 243 122, India. singh_br1762@rediffmail.com
Singh
V P
VP
Agarwal
Meenu
M
Sharma
Gautam
G
Chandra
Mudit
M
D000818:Animals; D002020:Buffaloes; D002448:Cell Adhesion; D002470:Cell Survival; D004912:Erythrocytes / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000502:physiology*; D006736:Horses; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D012475:Salmonella / Q000378:metabolism; D012480:Salmonella Infections; D019779:Salmonella enterica / Q000378:metabolism* / Q000472:pathogenicity; D012756:Sheep; D013045:Species Specificity
D006460:Hemolysin Proteins