title: Antioxidants, free radicals, storage proteins, puroindolines, and proteolytic activities in bread wheat (Triticum aestivum) seeds during accelerated aging.
abstract: Seeds of bread wheat were incubated at 40 degrees C and 100% relative humidity for 0, 3, 4, 6, and 10 days. The effects of accelerated aging on seed germinability and some biochemical properties of flour (carotenoid, free radical, and protein contents and proteolytic activity) and gluten (free radical content and flexibility) were investigated. Seed germinability decreased during aging, resulting in seed death after 10 days. A progressive decrease of carotenoid content, in particular, lutein, was observed, prolonging the incubation, whereas the free radical content increased in both flour and gluten. A degradation of soluble and storage proteins was found, associated with a marked increase of proteolytic activity and a loss of viscoelastic properties of gluten. On the contrary, puroindolines were quite resistant to the treatment. The results are discussed in comparison with those previously obtained during accelerated aging of durum wheat seeds.
authors.affiliation: Istituto per i Processi Chimico-Fisici, CNR, Area della Ricerca, via G. Moruzzi 1, I-56124 Pisa, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Calucci
authors.forename: Antonella
authors.initials: A
authors.lastname: Capocchi
authors.forename: Luciano
authors.initials: L
authors.lastname: Galleschi
authors.forename: Silvia
authors.initials: S
authors.lastname: Ghiringhelli
authors.forename: Calogero
authors.initials: C
authors.lastname: Pinzino
authors.forename: Franco
authors.initials: F
authors.lastname: Saviozzi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zandomeneghi
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001939:Bread; D002338:Carotenoids / Q000032:analysis; D004548:Elasticity; D010450:Endopeptidases / Q000032:analysis*; D005609:Free Radicals / Q000032:analysis*; D018525:Germination; D010940:Plant Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development*; D013997:Time Factors; D014908:Triticum / Q000737:chemistry*; D014783:Viscosity
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D010940:Plant Proteins; D002338:Carotenoids; D010450:Endopeptidases