Expression of C-reactive protein and heat-shock protein-70 in the lung epithelial cell line A549, in response to PM10 exposure.
Increased levels of C-reactive protein (CRP) and heat-shock protein-70 (Hsp70) in plasma are known to be associated with an increased risk of cardiovascular disease. In this study we have investigated the effects of environmental air pollution particles (PM10) and ultrafine carbon black (ufCB) on the expression of CRP and Hsp70 in the lung epithelial cell line, A549. After treatment with PM10 or ufCB the cells were found to have increased expression of CRP and Hsp70 localized in both the cell cytoplasm and nucleus. Analysis of the cell supernatants revealed that CRP and Hsp70 were present, suggesting secretion of both proteins in response to the particulate treatment. To investigate if the expression of CRP and Hsp70 was the result of free radical production, cells were treated with ufCB in the presence of antioxidants (NAL and Trolox). This revealed that antioxidants reduced the amount of CRP and Hsp70 secreted from the cells. These findings suggest that CRP and Hsp70 may be secreted from the lung epithelium as a result of oxidative stress and have important effects on the inflammatory response associated with inhalation of particulate matter.
Ramage
Lindsay
L
Biomedicine Research Group, School of Life Sciences, Napier University, Edinburgh, United Kingdom.
Guy
Keith
K
D000393:Air Pollutants / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000096:biosynthesis*; D002244:Carbon / Q000633:toxicity; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D010316:Particle Size; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D000393:Air Pollutants; D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D002244:Carbon; D002097:C-Reactive Protein