title: Transcriptional analysis of the groES-groEL1, groEL2, and dnaK genes in Corynebacterium glutamicum: characterization of heat shock-induced promoters.
abstract: The appropriate conditions to switch on the heat shock promoters in Corynebacterium glutamicum were defined by Northern blot analysis. Transcriptional patterns were characterized for the groEL2 gene and the groES-groEL1 and dnaK operons. Transcriptional start points of these genes were determined by primer extension analysis, allowing the identification of CIRCE and HAIR boxes close to the -10 and -35 regions of the promoters. The presence of both CIRCE and HAIR sequences within a single promoter (P-groEL2) in bacteria is described for the first time. In addition, the dnaK promoter showed -10 and -35 sequences similar to those recognized by SigH of Mycobacterium and SigR of Streptomyces close to a second transcription start region with -10 and -35 boxes typical of promoters for housekeeping genes.
authors.affiliation: Institute of Biotechnology of León (INBIOTEC). Science Park of León. Av. Real, 1, León, 24006, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Barreiro
authors.forename: Eva
authors.initials: E
authors.lastname: González-Lavado
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pátek
authors.forename: Juan-Francisco
authors.initials: JF
authors.lastname: Martín
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics; D018835:Chaperonin 10 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D017124:Conserved Sequence; D003352:Corynebacterium / Q000235:genetics* / Q000502:physiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D009161:Mycobacterium / Q000235:genetics; D009876:Operon; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics; D013302:Streptomyces / Q000235:genetics; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C120328:SigH protein, bacteria; D012808:Sigma Factor