title: Factors improving the propagation of Simkania negevensis strain Z in cell culture.
abstract: The purpose of the present study was to develop an optimal method for culturing Simkania negevensis. Centrifugation was effective for the propagation of S. negevensis, but sonication was not effective. The addition of cycloheximide to the culture medium significantly decreased the number of inclusions. Pretreatment of host monolayers with diethylaminoethyl-dextran or polyethylene glycol was detrimental. The most optimal conditions were centrifugation of the inocula onto untreated Vero cells, and culture in RPMI 1640 medium containing 10% fetal calf serum without cycloheximide or antimicrobial agents.
authors.affiliation: Department of Pediatrics, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan. tetsuyaf@hama-med.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Inoue
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ogawa
authors.forename: Sadashi
authors.initials: S
authors.lastname: Shiga
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Ouchi
authors.forename: Takehiko
authors.initials: T
authors.lastname: Ohzeki
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002498:Centrifugation; D002499:Centrifugation, Density Gradient; D002695:Chlamydiales / Q000187:drug effects / Q000254:growth & development*; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000737:chemistry*; D003513:Cycloheximide / Q000494:pharmacology*; D003637:DEAE-Dextran; D011092:Polyethylene Glycols; D011817:Rabbits; D013010:Sonication; D014709:Vero Cells
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D011092:Polyethylene Glycols; D003637:DEAE-Dextran; D003513:Cycloheximide