Evidence for the reactivity of fatty aldehydes released from oxidized plasmalogens with phosphatidylethanolamine to form Schiff base adducts in rat brain homogenates.
The vinyl ether bond of plasmalogens could be among the first target of free radicals attack. Consequently, because of their location in the membranes of cells, plasmalogens represent a first shield against oxidative damages by protecting other macromolecules and are often considered as antioxidant molecules. However, under oxidative conditions their disruption leads to the release of fatty aldehydes. In this paper, we showed using gas chromatography-mass spectrometry (GC-MS) analyses that fatty aldehydes released from plasmalogens after oxidation (UV irradiation and Fe2+/ascorbate) of cerebral cortex homogenates can generate covalent modifications of endogenous macromolecules such as phosphatidylethanolamine (PE), like the very reactive and toxic malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). These newly formed Schiff base adducts could be responsible for deleterious effects on cells thus making the protective role of plasmalogens potentially questionable.
Stadelmann-Ingrand
Sabrina
S
EA 3808, Faculté de Médecine Pharmacie, 34 rue du Jardin des Plantes, BP 199, 86005 Poitiers, France. sabrina.ingrand@univ-poitiers.fr
Pontcharraud
Raymond
R
Fauconneau
Bernard
B
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D017665:Hydroxyl Radical / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation; D008956:Models, Chemical; D018384:Oxidative Stress / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010955:Plasmalogens / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D026082:Singlet Oxygen / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D000447:Aldehydes; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010955:Plasmalogens; C001634:fatty aldehyde; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D007501:Iron; D001205:Ascorbic Acid