Differential diagnosis of gastrointestinal stromal tumor and other spindle cell tumors in the gastrointestinal tract based on immunohistochemical analysis.
To confirm the usefulness of an immunohistochemical panel of antibodies for KIT (c-kit/CD117), CD34, desmin, smooth-muscle actin (SMA), h-caldesmon (HCD), S-100 protein, neuron-specific enolase (NSE), and beta-catenin, 297 mesenchymal and peripheral nerve-sheath tumors of the gastrointestinal tract and intra-abdominal locations including 211 gastrointestinal stromal tumors (GISTs), 12 leiomyomas, 18 leiomyosarcomas, 17 solitary fibrous tumors (SFTs), 14 schwannomas, and 25 desmoid-type fibromatoses (DTFs) were analyzed immunohistochemically. Consistent (100%) immunoreactivity for KIT, CD34, desmin and S-100, and nuclear accumulation of beta-catenin were detected in GISTs, SFTs, smooth-muscle tumors, schwannomas, and DTFs, respectively. Immunoreactivity for SMA, HCD, and NSE was observed in a wide range of these tumors. In addition, 418 bone and soft tissue tumors were enrolled in this study for KIT immunostaining. As a result, a limited number of these tumors were KIT positive, including synovial sarcoma that showed morphological similarity to GISTs. These findings suggest that KIT, CD34, desmin, S-100, and beta-catenin are key markers for clinical diagnosis of GISTs and other spindle cell tumors that may involve the gastrointestinal tract, whereas SMA, HCD, and NSE have only limited value.
Yamaguchi
Umio
U
Divisions of Orthopaedic Oncology, Pathology and Clinical Laboratory, National Cancer Centre Hospital and Research Institute, Tokyo, Japan.
Hasegawa
Tadashi
T
Masuda
Taizo
T
Sekine
Shigeki
S
Kawai
Akira
A
Chuman
Hirokazu
H
Shimoda
Tadakazu
T
D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000175:diagnosis / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D003937:Diagnosis, Differential; D005770:Gastrointestinal Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018218:Neoplasms, Fibrous Tissue / Q000175:diagnosis / Q000378:metabolism; D009379:Neoplasms, Muscle Tissue / Q000175:diagnosis / Q000378:metabolism; D018317:Nerve Sheath Neoplasms / Q000175:diagnosis / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D012509:Sarcoma / Q000175:diagnosis / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D003893:Desmin; D009418:S100 Proteins; D019009:Proto-Oncogene Proteins c-kit