title: Recovery of neutrophil count by ganciclovir in patients with chronic idiopathic neutropenia associated with cytomegalovirus infection in bone marrow stromal cells.
abstract: Patients with chronic idiopathic neutropenia (CIN) usually show chronic inflammation in their bone marrow stromal cells, but the etiologies of this disorder are still unknown. We suspected viral infection of the bone marrow stromal cells and used polymerase chain reaction (PCR) analysis to test for cytomegalovirus (CMV) infection in 4 patients with CIN and in 6 healthy volunteers. Peripheral blood mononuclear cells did not yield signals in any of the patients or healthy volunteers, but PCR analysis for CMV DNA in bone marrow stromal cells showed positive signals in 2 of the patients with CIN. CMV DNA was not detected in the other 2 patients or in the healthy volunteers. The 2 patients who were positive for stromal CMV received 500 mg/day ganciclovir for 14 days. After this treatment, the PCR band showing the presence of stromal CMV disappeared, and the neutrophil numbers of the 2 patients who received this viral eradication therapy normalized 6 months after treatment. In conclusion, the etiology in some cases of CIN is speculated to be infection by CMV in bone marrow stromal cells, and treatment with ganciclovir may be effective in treating such patients.
authors.affiliation: Department of Internal Medicine, Higashi Sapporo Hospital, Shiroishiku Higashisapporo, Sapporo, Japan. yhirayama@hsh.or.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Sumio
authors.initials: S
authors.lastname: Sakamaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tsuji
authors.forename: Hiroki
authors.initials: H
authors.lastname: Chiba
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Rishu
authors.initials: R
authors.lastname: Takimoto
authors.forename: Junji
authors.initials: J
authors.lastname: Kato
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Niitsu
mesh_terms: D000368:Aged; D001854:Bone Marrow Cells / Q000821:virology; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis / Q000188:drug therapy; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015774:Ganciclovir / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000188:drug therapy / Q000821:virology*; D009504:Neutrophils / Q000187:drug effects; D016133:Polymerase Chain Reaction; D017154:Stromal Cells / Q000821:virology*
chemical_list: D004279:DNA, Viral; D015774:Ganciclovir