title: Induction of c-fos expression in the mouse brain associated with hyperalgesia induced by intrathecal injection of protein kinase C activator.
abstract: Here, we found that a single intrathecal (i.t.) administration of a protein kinase C (PKC) activator, phorbol 12,13-dibutyrate (PDBu), induced pain-like behaviors in mice. Furthermore, i.t.-administered PDBu caused the increased c-fos-like immunoreactivity in the parafascicular nuclei (PF), amygdala and cingulate cortex (CG), but not hippocampus. These findings suggest that the stimulation of spinal PKC results in an enhancement of neuronal activity in the PF, amygdala and CG associated with hyperalgesia.
authors.affiliation: Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan. narita@hoshi.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Narita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imai
authors.forename: Kousei
authors.initials: K
authors.lastname: Oe
authors.forename: Michiko
authors.initials: M
authors.lastname: Narita
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kubota
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yajima
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000679:Amygdala / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators; D006179:Gyrus Cinguli / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D007278:Injections, Spinal; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology
chemical_list: D020536:Enzyme Activators; D016760:Proto-Oncogene Proteins c-fos; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C