Towards a structural classification of phosphate binding sites in protein-nucleotide complexes: an automated all-against-all structural comparison using geometric matching.
A method is described for the rapid comparison of protein binding sites using geometric matching to detect similar three-dimensional structure. The geometric matching detects common atomic features through identification of the maximum common sub-graph or clique. These features are not necessarily evident from sequence or from global structural similarity giving additional insight into molecular recognition not evident from current sequence or structural classification schemes. Here we use the method to produce an all-against-all comparison of phosphate binding sites in a number of different nucleotide phosphate-binding proteins. The similarity search is combined with clustering of similar sites to allow a preliminary structural classification. Clustering by site similarity produces a classification of binding sites for the 476 representative local environments producing ten main clusters representing half of the representative environments. The similarities make sense in terms of both structural and functional classification schemes. The ten main clusters represent a very limited number of unique structural binding motifs for phosphate. These are the structural P-loop, di-nucleotide binding motif [FAD/NAD(P)-binding and Rossman-like fold] and FAD-binding motif. Similar classification schemes for nucleotide binding proteins have also been arrived at independently by others using different methods.
Brakoulias
Andreas
A
Department of Biochemistry & Molecular Biology, University College London, Gower Street, London, England.
Jackson
Richard M
RM
D000465:Algorithms; D020816:Amino Acid Motifs; D001665:Binding Sites; D016000:Cluster Analysis; D030562:Databases, Protein; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D046911:Macromolecular Substances; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D010710:Phosphates / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D016914:Ribonuclease H / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000737:chemistry; D013329:Structure-Activity Relationship
D008024:Ligands; D046911:Macromolecular Substances; D009711:Nucleotides; D010446:Peptide Fragments; D010710:Phosphates; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D016914:Ribonuclease H