[Effect of grape procyanidins on the apoptosis and mitochondrial transmembrane potential of thymus cells].
OBJECTIVE
Investigating the effect of grape procyanidins(GPC) on the apoptosis and mitochondrial transmembrane potential of thymus cells damaged by H2O2.


METHODS
Thymus cells of mouse were cultured in vitro. The cells were pretreated by GPC and then damaged by 125 mumol/L H2O2 for two hours. The levels of apoptosis and mitochondrial transmembrane potential were analysed by flow cytometry.


RESULTS
Apoptosis rate and the level of mitochondrial transmembrane potential of positive group were (29.53 +/- 3.8)% and 27.24 +/- 2.2 (D value) respectively. After pretreatment of 50 mg/L GPC, the levels of apoptosis and mitochondrial transmembrane potential were (8.61 +/- 1.2)% and 87.55 +/- 4.7(D value). The differences between the two groups were significant (P < 0.05).


CONCLUSIONS
GPC can inhibit apoptosis and decrease of mitochondrial transmembrane potential of thymus cells damaged by oxygen free radical.
Li
Lei
L
Nutrition Institute, Medical College of Qingdao University, Qingdao 266021, China.
Zhong
Jinyi
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000473:pathology; D018384:Oxidative Stress; D044945:Proanthocyanidins; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013950:Thymus Gland / Q000473:pathology* / Q000502:physiology; D027843:Vitis / Q000737:chemistry
D000975:Antioxidants; D044946:Biflavonoids; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin; D006861:Hydrogen Peroxide