Lymphotoxin and TNF produced by B cells are dispensable for maintenance of the follicle-associated epithelium but are required for development of lymphoid follicles in the Peyer's patches.
Organogenesis of Peyer's patches (PP), follicle-associated epithelium, and M cells is impaired in mice lacking B cells. At the same time, lymphotoxin (LT) and TNF are known to be critical for the development of PP. To directly address the function of LT and TNF expressed by B cells in the maintenance of PP structure, we studied the de novo formation of PP in B cell-deficient mice after the transfer of bone marrow from mice with targeted mutations in LT, TNF, or their combinations. We found that although the compartmentalization of T and B cell zones and development of follicular dendritic cells were affected by the lack of B cell-derived LT and TNF, the development of follicle-associated epithelium and M cells in PP was completely independent of LT/TNF production by B cells.
Tumanov
Alexei V
AV
The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
Kuprash
Dmitry V
DV
Mach
Julie A
JA
Nedospasov
Sergei A
SA
Chervonsky
Alexander V
AV
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002451:Cell Compartmentation; D020566:Dendritic Cells, Follicular / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D008233:Lymphotoxin-alpha / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000166:cytology* / Q000648:ultrastructure; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha