title: Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate.
abstract: Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 microm positioned at the outlet of the separation channel was used as a working electrode for amperometric detection. Factors influencing separation and detection were investigated and optimized. Results show that acetaminophen and p-aminophenol can be well separated within 35 s with RSD-1 (approximately 0.1 fmol) at S/ N=3. This method has been successfully applied to the detection of traces of p-aminophenol in paracetamol tablets.
authors.affiliation: The State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Department of Chemistry, Nanjing University, 210093 Nanjing, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: He
authors.forename: A L
authors.initials: AL
authors.lastname: Liu
authors.forename: X H
authors.initials: XH
authors.lastname: Xia
mesh_terms: D000082:Acetaminophen / Q000032:analysis* / Q000737:chemistry; D000627:Aminophenols / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D006868:Hydrolysis; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000627:Aminophenols; D004129:Dimethylpolysiloxanes; D011108:Polymers; D000082:Acetaminophen