MAK-5 treatment enhances the nerve growth factor-mediated neurite outgrowth in PC12 cells.
The effects of an ayurvedic compound (MAK-5) alone or together with nerve growth factor (NGF) on the neurite outgrowth of PC12 cells was studied. PC12 cells treated with NGF alone showed a clear neurite outgrowth with a decrease of the proliferation at the dose higher than 5 ng/ml. MAK-5 alone does not induce significant neurite outgrowth in the PC12 cells and does not decrease the proliferation. The PC12 cells treated with NGF supplemented with MAK-5 showed a well-evident morphological differentiation also at low doses of NGF (less than 5 ng/ml), however, the proliferation does not decrease. We suggest that MAK-5 could contain some differentiating agents that are able to potentiate NGF inducing neuronal differentiation in PC12 cells without decreasing the cell proliferation.
Rodella
Luigi
L
Department of Biomedical Sciences and Biotechnology, Division of Human Anatomy, University of Brescia, Via Valsabbina 19, 25124 Brescia, Italy.
Borsani
Elisa
E
Rezzani
Rita
R
Lanzi
Roberto
R
Lonati
Claudio
C
Bianchi
Rossella
R
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008515:Medicine, Ayurvedic; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D028321:Plant Preparations; D051381:Rats
C476033:Maharishi Amrit Kalash; D010936:Plant Extracts; D028321:Plant Preparations; D020932:Nerve Growth Factor