Two-dimensional infrared spectroscopy of antiparallel beta-sheet secondary structure.
We investigate the sensitivity of femtosecond Fourier transform two-dimensional infrared spectroscopy to protein secondary structure with a study of antiparallel beta-sheets. The results show that 2D IR spectroscopy is more sensitive to structural differences between proteins than traditional infrared spectroscopy, providing an observable that allows comparison to quantitative models of protein vibrational spectroscopy. 2D IR correlation spectra of the amide I region of poly-l-lysine, concanavalin A, ribonuclease A, and lysozyme show cross-peaks between the IR-active transitions that are characteristic of amide I couplings for polypeptides in antiparallel hydrogen-bonding registry. For poly-l-lysine, the 2D IR spectrum contains the eight-peak structure expected for two dominant vibrations of an extended, ordered antiparallel beta-sheet. In the proteins with antiparallel beta-sheets, interference effects between the diagonal and cross-peaks arising from the sheets, combined with diagonally elongated resonances from additional amide transitions, lead to a characteristic "Z"-shaped pattern for the amide I region in the 2D IR spectrum. We discuss in detail how the number of strands in the sheet, the local configurational disorder in the sheet, the delocalization of the vibrational excitation, and the angle between transition dipole moments affect the position, splitting, amplitude, and line shape of the cross-peaks and diagonal peaks.
Demirdöven
Nurettin
N
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Cheatum
Christopher M
CM
Chung
Hoi Sung
HS
Khalil
Munira
M
Knoester
Jasper
J
Tokmakoff
Andrei
A
D000577:Amides / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D006860:Hydrogen Bonding; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D000577:Amides; D009211:Myoglobin; D010455:Peptides; D011506:Proteins; D003208:Concanavalin A; D011107:Polylysine; D012259:Ribonuclease, Pancreatic; D009113:Muramidase