Molecular adsorption: early stage surface exploration.
In this study, the atomic force microscope has been employed in force spectroscopy mode to gain information on the interaction between long mucin molecules and a positively charged surface during the first few seconds of interaction. Recent studies have revealed that negatively charged mucin molecules introduced to a positively charged surface are kinetically trapped and bind very rapidly, assuming non-equilibrium conformations. This systematic study of surface dwell times has revealed that significant differences exist in mucin adsorption during the first three seconds of introduction to the surface and provides direct evidence of molecular rearrangement for several seconds before trapping occurs. Limited interactions were recorded at dwell times of less than one second, with increased molecular rearrangement observed between 1.5 and 2.25 s. Increasing the surface dwell time beyond this critical limit caused rupture of the tip-tethered mucin molecules during the retract cycle of the cantilever. All subsequent recorded events, at increased dwell times up to 3s, revealed events at much reduced distances from the point of contact between the mucin functionalised-cantilever and the positively charged surface.
Brayshaw
Debra J
DJ
H.H. Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol BS8 1TL, UK. debra.brayshaw@bristol.ac.uk
Berry
Monica
M
McMaster
Terence J
TJ
D000327:Adsorption; D006801:Humans; D018625:Microscopy, Atomic Force; D009077:Mucins / Q000737:chemistry*
D009077:Mucins