[Antiretinal antibodies (ARA) in patients with endogenous posterior uveitis in indirect immunofluorescence test].
OBJECTIVE
To determine the types of antiretinal antibodies (ARA) in patients with endogenous posterior uveitis in active stage of the disease using indirect immunofluorescence test.


METHODS
50 patients--29 women (58%) and 21 men (42%) in age 19-70 years with idiopathic posterior uveitis were examined. In all cases the ARA were determined by indirect immunofluorescence test on normal monkey retina, as a substrate and FITC-labelled goat's anti-human IgA, G, M serum (Euroimmun-Germany). The control serum was obtained from 50 blood donors: 20 women (40%) and 30 men (60%) in age 20-64 years.


RESULTS
Indirect immunofluorescence test showed the reaction with the outer retinal segments in 97.5% of cases. In 9 patients (22.5%) with positive serum antinuclear antibodies (ANA), the addictive reaction with the nuclei of both nuclear layers of retina was present. In one subject (2.5%), the diffuse fluorescence of all layers of retina was detected.


CONCLUSIONS
The most common type of ARA in patients with endogenous posterior uveitis were antibodies against the outer layers of retina, cytoplasm of photoreceptors and cells of outer nuclear layer.
Kubicka-Trzaska
Agnieszka
A
Katedry i Kliniki Okulistyki Collegium Medicum Uniwersytetu Jagiellońskiego w Krakowie.
D000208:Acute Disease; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D012160:Retina / Q000276:immunology*; D015866:Uveitis, Posterior / Q000276:immunology*
D001323:Autoantibodies