Detection of enteroviruses in treated drinking water.
This study deals with the routine monitoring of drinking water for the presence of enteroviruses, over a period of 1 year. A rapid and simple method was employed for the simultaneous detection and typing of enteroviruses in large-volume water samples. This included an integrated cell culture/nested PCR approach, followed by restriction enzyme analysis. The two drinking water supplies studied were derived from acceptable quality surface water sources using treatment processes, which conform to international specifications for the production of safe drinking water. Enteroviruses (predominantly coxsackie B viruses) were detected in 11% and 16% of the drinking water samples from two treatment plants, respectively. This study confirms that acceptable water quality indicators do not necessarily reflect the virus content of drinking water.
Vivier
J C
JC
Department of Medical Virology, Institute of Pathology, University of Pretoria, P.O. Box 2034, 0001, South Africa. cvivier@ages-group.com
Ehlers
M M
MM
Grabow
W O K
WO
D004279:DNA, Viral / Q000032:analysis; D029822:Enterovirus B, Human / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011786:Quality Control; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D018508:Water Purification / Q000379:methods*
D004279:DNA, Viral