Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells.
We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10(-6) to 10(-3) M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10(-4)M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10(-8) M)-induced increases in [cAMP]c and [Ca2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.
Katoh
K
K
Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. kato@bios.tohoku.ac.jp
Matsuda
A
A
Ishigami
A
A
Yonekura
S
S
Ishiwata
H
H
Chen
C
C
Obara
Y
Y
D000818:Animals; D001559:Benzhydryl Compounds; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D000242:Cyclic AMP / Q000378:metabolism; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000187:drug effects / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012756:Sheep; D013997:Time Factors; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002118:Calcium; D000242:Cyclic AMP; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D010636:Phenols; D012333:RNA, Messenger; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds