Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA.
Small interfering RNA (siRNA)-induced RNA degradation has been used recently as an antivirus agent to inhibit specific virus replication. This report shows that 21 nt duplexes of siRNA of the influenza virus M gene can cause specific inhibition of influenza virus matrix (M1) protein expression in transfected 293T cells. Furthermore, it is shown that a lentivirus vector can be used to effectively deliver M gene siRNAs into Madin-Darby canine kidney cells and can cause specific inhibition of M1 protein expression and influenza virus replication. Therefore, lentivirus-mediated delivery of siRNA and gene silencing can be used in studying the specific functions of virus genes in replication and may have a potential therapeutic application.
Hui
Eric Ka-Wai
EK
Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
Yap
Ee Ming
EM
Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
An
Dong Sung
DS
Departments of Microbiology, Immunology, and Molecular Genetics and Medicine, UCLA AIDS Institute, Los Angeles, CA 90095, USA.
Chen
Irvin S Y
ISY
Departments of Microbiology, Immunology, and Molecular Genetics and Medicine, UCLA AIDS Institute, Los Angeles, CA 90095, USA.
Nayak
Debi P
DP
Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007668:Kidney; D016086:Lentivirus / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
D034741:RNA, Small Interfering; D012367:RNA, Viral; D014763:Viral Matrix Proteins