Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D.
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.
Minoda
Ayumi
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, 113-0032 Japan.
Sakagami
Rei
R
Yagisawa
Fumi
F
Kuroiwa
Tsuneyoshi
T
Tanaka
Kan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods*; D002467:Cell Nucleus / Q000235:genetics*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D018744:DNA, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000172:deficiency / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009962:Orotate Phosphoribosyltransferase / Q000172:deficiency / Q000235:genetics; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000172:deficiency / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D000461:Rhodophyta / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics*
D003470:Culture Media; D018744:DNA, Plant; D009097:Multienzyme Complexes; D009963:Orotic Acid; C027157:uridine 5'-monophosphate synthase; C001242:5-fluoroorotic acid; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase