title: The slit receptor Rig-1/Robo3 controls midline crossing by hindbrain precerebellar neurons and axons.
abstract: During development, precerebellar neurons migrate dorsoventrally from the rhombic lip to the floor plate. Some of these neurons cross the midline while others stop. We have identified a role for the slit receptor Rig-1/Robo3 in directing this process. During their tangential migration, neurons of all major hindbrain precerebellar nuclei express high levels of Rig-1 mRNA. Rig-1 expression is rapidly downregulated as their leading process crosses the floor plate. Interestingly, most precerebellar nuclei do not develop normally in Rig-1-deficient mice, as they fail to cross the midline. In addition, inferior olivary neurons, which normally send axons into the contralateral cerebellum, project ipsilaterally in Rig-1 mutant mice. Similarly, neurons of the lateral reticular nucleus and basilar pons are unable to migrate across the floor plate and instead remain ipsilateral. These results demonstrate that Rig-1 controls the ability of both precerebellar neuron cell bodies and their axons to cross the midline.
authors.affiliation: CNRS UMR7102, Université Paris 6, Bâtiment B, Case 12, 9 Quai Saint-Bernard, 75005 Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Marillat
authors.forename: Christelle
authors.initials: C
authors.lastname: Sabatier
authors.forename: Vieri
authors.initials: V
authors.lastname: Failli
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsunaga
authors.forename: Constantino
authors.initials: C
authors.lastname: Sotelo
authors.forename: Marc
authors.initials: M
authors.lastname: Tessier-Lavigne
authors.forename: Alain
authors.initials: A
authors.lastname: Chédotal
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D005333:Fetus; D007839:Functional Laterality / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009847:Olivary Nucleus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011149:Pons / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D000097905:Roundabout Proteins
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C118348:Robo3 protein, mouse; D000097905:Roundabout Proteins; C120010:Slit1 protein, mouse