title: Phospholipase-mediated preparation of 1-ricinoleoyl-2-acyl-sn-glycero-3-phosphocholine from soya and egg phosphatidylcholine.
abstract: 1-Ricinoleoyl-2-acyl-sn-glycero-3-phosphocholine was prepared by incorporating ricinoleic acid completely in the sn-1 position of egg and soya phosphatidylcholine (PC) using immobilized phospholipase A(1) as the catalyst. The optimum reaction conditions for maximum incorporation of ricinoleic acid into PC through transesterification were 10% (w/w) immobilized enzyme (116 mg), a 1:5 mol ratio of PC (soya, 387 mg; egg, 384 mg) to methyl ricinoleate (780 mg) at 50 degrees C for 24 h in hexane.
authors.affiliation: Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad-500 007, India.
authors.forename: T
authors.initials: T
authors.lastname: Vijeeta
authors.forename: J R C
authors.initials: JR
authors.lastname: Reddy
authors.forename: B V S K
authors.initials: BV
authors.lastname: Rao
authors.forename: M S L
authors.initials: MS
authors.lastname: Karuna
authors.forename: R B N
authors.initials: RB
authors.lastname: Prasad
mesh_terms: D004531:Eggs / Q000032:analysis*; D010713:Phosphatidylcholines / Q000138:chemical synthesis*; D010741:Phospholipases A / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D010741:Phospholipases A