title: Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resistant human colon carcinoma LoVo/5-FU cells.
abstract: OBJECTIVE
  To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).
  
  
  METHODS
  After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcription-polymerase chain reaction and the expression of p53 protein was examined by Western blotting.
  
  
  RESULTS
  The reversal activity after treatment with wide-type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.
  
  
  CONCLUSIONS
  Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.
authors.affiliation: Department of Abdominal Surgery, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China. yuzhiwei1974@163.com
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Xin-Shu
authors.initials: XS
authors.lastname: Dong
authors.forename: Ji
authors.initials: J
authors.lastname: Tao
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Yao
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Yin
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Song-Bin
authors.initials: SB
authors.lastname: Fu
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000230:Adenocarcinoma; D000256:Adenoviridae / Q000235:genetics; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D006801:Humans; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000964:Antimetabolites, Antineoplastic; D016159:Tumor Suppressor Protein p53; D005472:Fluorouracil