Multiple sclerosis as a generalized CNS disease--comparative microarray analysis of normal appearing white matter and lesions in secondary progressive MS.
We used microarrays to compare the gene expression profile in active lesions and donor-matched normal appearing white matter (NAWM) from brain autopsy samples of patients with secondary progressive multiple sclerosis (MS) with that from controls who died from non-neurological diseases. The 123 genes in lesions, and 47 genes in NAWM(MS) were differentially expressed. Lesions distinguished from NAWM(MS) by a higher expression of genes related to immunoglobulin synthesis and neuroglial differentiation, while cellular immune response elements were equally dysregulated in both tissue compartments. Current results provide molecular evidence of a continuum of dysfunctional homeostasis and inflammatory changes between lesions and NAWM(MS), and support the concept of MS pathogenesis being a generalised process that involves the entire CNS.
Lindberg
Raija L P
RL
Clinical Neuroimmunology Laboratory, Departments of Research and Neurology, University Hospitals Basel, Pharmazentrum, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. Raija.Lindberg@unibas.ch
De Groot
Corline J A
CJ
Certa
Ulrich
U
Ravid
Rivka
R
Hoffmann
Francine
F
Kappos
Ludwig
L
Leppert
David
D
D001921:Brain / Q000473:pathology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D009103:Multiple Sclerosis / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction