Mild impairment of motor nerve repair in mice lacking PTP-BL tyrosine phosphatase activity.
Mouse PTP-BL is a large, nontransmembrane protein tyrosine phosphatase of unclear physiological function that consists of a KIND domain, a FERM domain, five PDZ domains, and a COOH-terminal catalytic PTP domain. PTP-BL and its human ortholog PTP-BAS have been proposed to play a role in the regulation of microfilament dynamics, cytokinesis, apoptosis, and neurite outgrowth. To investigate the biological function of PTP-BL enzyme activity, we have generated mice that lack the PTP-BL PTP moiety. These PTP-BL(DeltaP/DeltaP) mice are viable and fertile and do not present overt morphological alterations. Although PTP-BL is expressed in most hematopoietic cell lineages, no alterations of thymocyte development in PTP-BL(DeltaP/DeltaP) mice could be detected. Sciatic nerve lesioning revealed that sensory nerve recovery is unaltered in these mice. In contrast, a very mild but significant impairment of motor nerve repair was observed. Our findings exclude an essential role for PTP-BL as a phosphotyrosine phosphatase and rather are in line with a role as scaffolding or anchoring molecule.
Wansink
Derick G
DG
Department of Cell Biology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, 6525 GA Nijmegen, The Netherlands.
Peters
Wilma
W
Schaafsma
Iris
I
Sutmuller
Roger P M
RP
Oerlemans
Frank
F
Adema
Gosse J
GJ
Wieringa
Bé
B
van der Zee
Catharina E E M
CE
Hendriks
Wiljan
W
D000483:Alleles; D000818:Animals; D001369:Axons / Q000378:metabolism; D001835:Body Weight; D005260:Female; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000378:metabolism* / Q000473:pathology*; D009409:Nerve Crush; D009416:Nerve Regeneration; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012584:Sciatic Nerve / Q000502:physiology; D017384:Sequence Deletion / Q000235:genetics
D012333:RNA, Messenger; C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases; C516743:Ptpn13 protein, mouse