title: Purification and characterization of recombinant Staphylococcus haemolyticus DNA gyrase and topoisomerase IV expressed in Escherichia coli.
abstract: The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating type II topoisomerases of a coagulase-negative staphylococcal species.
authors.affiliation: Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. Joel.Bronstein@pfizer.com
authors.forename: Joel C
authors.initials: JC
authors.lastname: Bronstein
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Olson
authors.forename: Kristin
authors.initials: K
authors.lastname: LeVier
authors.forename: Mark
authors.initials: M
authors.lastname: Tomilo
authors.forename: Peter C
authors.initials: PC
authors.lastname: Weber
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D027101:DNA Topoisomerase IV / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D041162:Staphylococcus haemolyticus / Q000187:drug effects / Q000201:enzymology*
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase