Animal and human models for studying effects of drugs on intestinal fluid transport in vivo.
The understanding of the physiology and pathophysiology of intestinal fluid transport has been derived from animal and human models of normal and perturbed intestines. This understanding helped in designing drugs and changing the composition of oral rehydration solutions in a targeted manner to affect intestinal fluid absorption/secretion that was tested both in vitro and in vivo before embarking on clinical trials. In this review, in vivo techniques used to study water transport in both animal and human models are described. In particular, steady state intestinal perfusion techniques, closed segment techniques, fistulous animal models, balance study models, enteropooling models, and isotope tracer models are reviewed. Advantages and drawbacks of each technique and examples where drug effects have been studied in a particular model are provided.
Mourad
Fadi H
FH
Department of Internal Medicine, Faculty of Medicine, American University of Beirut Medical Center, P.O. Box 113-6044, Hamra 110-32090, Beirut, Lebanon. fmourad@aub.edu.lb
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001834:Body Water / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D023421:Models, Animal; D008954:Models, Biological; D010477:Perfusion; D015532:Rehydration Solutions / Q000378:metabolism*
D015532:Rehydration Solutions