title: Hyaluronan release from Streptococcus pyogenes: export by an ABC transporter.
abstract: Capsular hyaluronan of Streptococcus pyogenes is synthesized at the protoplast membrane. It is widely assumed that hyaluronan is exported by the synthase itself and that no additional protein is required for transfer through plasma membranes. However, we produced an insertional mutation that reduced the mucoid phenotype, hyaluronan production, and capsule formation. Nucleotide sequence analysis of the insertion site identified a gene coding for a protein with an ATP-binding cassette (ABC) that belonged to an ABC transporter system and was located next to the hyaluronan synthesis genes. The mucoid phenotype was reconstituted by complementation with DNA encoding the ABC transporter system. These results indicated that an ABC transporter was required for efficient capsule production.
authors.affiliation: Muenster University Hospital, Institute of Physiological Chemistry and Pathobiochemistry, Waldeyerstr. 15, D-48129, Münster, Germany.
authors.forename: Galina
authors.initials: G
authors.lastname: Ouskova
authors.forename: Barbara
authors.initials: B
authors.lastname: Spellerberg
authors.forename: Peter
authors.initials: P
authors.lastname: Prehm
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D016667:Bacterial Capsules / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D013297:Streptococcus pyogenes / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014166:Transferases / Q000235:genetics / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D006820:Hyaluronic Acid; D004247:DNA; D014166:Transferases; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases