[Dissociation action of cationic peptides with hydrophobic radicals on functional coupling between serpentine type receptors and GTP-binding proteins].
The coupling of hormone-activated receptor and heterotrimeric G protein is an important step of the signal transduction through adenylyl cyclase signal system (ACS). The numerous literature data and own results show that G protein-interacting regions, that are localized in cytoplasmic loops of receptors, have considerable positive charge, can form amphiphilic alpha-helices and are tightly associated with the membrane. We studied the influence of model cationic peptides on both basal and stimulated by hormones and nonhormonal agents adenylyl cyclase (AC) activity and on GTP binding activity of heterotrimeric G proteins in skeletal muscles of rats and smooth muscles of mollusc Anodonta cygnea. Peptides with hydrophobic radicals of caprinoyl acid (C10): Lys(C10)-His-Glu-Lys-Lys-(C10)-His-Glu-Lys-Lys(C10)-His-Glu-Lys-Lys(C10)- His-Glu-Lys-Ala-amide (peptide I), Cys-Lys(C10)-X-Tyr-Lys-Ala-Lys7-Trp-Lys-amide (II), Cys-X-Trp-Lys-Lys(C10)-Lys2-Lys(C10)-Lys3-Lys(C10)-Tyr-Lys-Lys(C10)-Lys-Lys- amide (III), where X--epsilon-aminocaproyl acid residue, were synthesized by solid-phase methodology. IC50 values for inhibiting the influence of peptides on serotonin-(molluscs) and isoproterenol-stimulated (rats) AC activity were: for peptide I--56 and 70 mkM, for peptide II--32 and 47 mkM, for peptide III--22 and 28 mkM, respectively. At the same time the peptides weakly decreased AC activity stimulated by nonhormonal agents (NaF, Gpp[NH]p, forskolin). Peptides I--III stimulated basal activity of the enzyme in both investigated tissues. The maximum stimulating effects (28--52%) of the peptides were observed at their concentration 10 mkM. Peptides (10--100 mkM) increased Gpp[NH]p binding in plasma membranes of mollusc and rat muscles and strongly decreased the influence of the hormones on the binding. Based on the obtained data we supposed that cationic peptides with hydrophobic radicals mimic G protein-binding regions of the receptors and can be involved in the regulation of functional coupling between the receptors and G proteins.
Shpakov
A O
AO
I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS. shpakov@hormone.ief.spb.su
Gur'ianov
I A
IA
Vorob'ev
V I
VI
Avdeeva
E V
EV
Kuznetsova
L A
LA
Plesneva
S A
SA
Chubeĭ
N M
NM
Pertseva
M N
MN
Vlasov
G P
GP
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism; D005576:Colforsin; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007545:Isoproterenol; D008974:Mollusca; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
D002412:Cations; D010455:Peptides; D005576:Colforsin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol