title: Synergistic effect of antioxidant phenolic acids on human phenolsulfotransferase activity.
abstract: Sulfate conjugation by phenolsulfotransferases (PSTs) is an important process in the detoxification of xenobiotics and endogenous compounds. There are two forms of PSTs for the sulfation of small phenols (PST-P) and monoamines (PST-M). Phenolic acids are known to increase the activities of PST-P and PST-M. The purpose of this study is to investigate the synergistic effect of the combinations of phenolic acids on human PSTs activities. The combinations of p-hydroxybenzoic acid, gentisic acid, ferulic acid, gallic acid, and coumaric acid in a random order for their effects on PSTs activities were evaluated at concentrations of 2.5, 5.0, and 7.5 microM. The PST-M activity was significantly increased when gentisic acid was combined with each of the other phenolic acids. When p-hydroxybenzoic acid was combined with each of the other phenolic acids, a synergistic effect with respect to the promotion of PST-P activity was obtained. A potential synergistic effect for the PST-P activity was also found in the following combination: p-hydroxybenzoic acid + gallic acid + gentisic acid, p-hydroxybenzoic acid + gallic acid + m-coumaric acid, p-hydroxybenzoic acid + o-coumaric acid + p-coumaric acid, p-hydroxybenzoic acid + o-coumaric acid + m-coumaric acid, gallic acid + gentisic acid + p-coumaric acid, and gallic acid + o-coumaric acid + m-coumaric acid. Therefore, the activities of both forms of PSTs can be promoted by all of these combinations of phenolic acids. These results provide a better understanding regarding the effect of phenolic acids on human PSTs activities, as well as more information on the intake of antioxidant phenolic acids for human health.
authors.affiliation: Department of Food Science, National Chung-Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan.
authors.forename: Chi-Tai
authors.initials: CT
authors.lastname: Yeh
authors.forename: Ping-Hsiao
authors.initials: PH
authors.lastname: Shih
authors.forename: Gow-Chin
authors.initials: GC
authors.lastname: Yen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D015239:Arylsulfotransferase / Q000378:metabolism*; D003373:Coumaric Acids / Q000494:pharmacology; D004357:Drug Synergism; D005707:Gallic Acid / Q000494:pharmacology; D005841:Gentisates / Q000494:pharmacology; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D005841:Gentisates; D062385:Hydroxybenzoates; D005707:Gallic Acid; C004999:ferulic acid; D015239:Arylsulfotransferase; C017616:phenolic acid; C010925:2,5-dihydroxybenzoic acid