title: Structural characterization of the nonameric assembly of an Archaeal alpha-L-fucosidase by synchrotron small angle X-ray scattering.
abstract: alpha-l-Fucosidase is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of carbohydrate moieties in glycoproteins. The first alpha-l-fucosidase from Archaea was recently identified in the genome of the hyperthermophile Sulfolobus solfataricus; the enzyme is encoded by two open reading frames separated by a -1 frameshift. A preliminary biochemical and biophysical characterization of this extremophile enzyme has been carried out both in solution, through small angle X-ray scattering experiments, and in the crystalline state, showing an unusual oligomeric assembly resulting from the association of nine subunits, endowed with 3-fold molecular symmetry.
authors.affiliation: X-ray Structural Biology Unit--National Institute for Cancer Research (IST), Largo R. Benzi 10, 16132 Genoa, Italy.
authors.forename: Camillo
authors.initials: C
authors.lastname: Rosano
authors.forename: Simone
authors.initials: S
authors.lastname: Zuccotti
authors.forename: Beatrice
authors.initials: B
authors.lastname: Cobucci-Ponzano
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Mazzone
authors.forename: Mosè
authors.initials: M
authors.lastname: Rossi
authors.forename: Marco
authors.initials: M
authors.lastname: Moracci
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Petoukhov
authors.forename: Dmitri I
authors.initials: DI
authors.lastname: Svergun
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D008958:Models, Molecular; D008970:Molecular Weight; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D012996:Solutions; D016944:Sulfolobus / Q000201:enzymology*; D017356:Synchrotrons; D014961:X-Ray Diffraction / Q000379:methods*; D005644:alpha-L-Fucosidase / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012996:Solutions; D005644:alpha-L-Fucosidase