Detection of cellulolytic bacteria from the human colon.
The main representatives of bacteria in the human colon were investigated by specific PCR and denaturing gradient gel electrophoresis (DGGE). Prevalent in both cases were species of Bifidobacterium, Clostridium, Bacteroides, Faecalibacterium and Eubacterium. Simultaneously, cellulolytic bacteria were isolated from the human feces. The largest proportion was represented by ruminococcus-like isolates. Their presence was confirmed both by PCR and DGGE methods; the latter one was able to give more comprehensive data about the composition of bacterial population in the human colon chyme.
Kopecný
J
J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia. kopecny@iapg.cas.cz
Hajer
J
J
Mrázek
J
J
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001439:Bacteroides / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003106:Colon / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005051:Eubacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045857:Ruminococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002482:Cellulose; D002480:Cellulase