Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract.
A haemorrhagic protein toxin (SA-HT) was isolated and purified from the spine extract of the Indian venomous butterfish, S. argus Linn, by two step ion exchange chromatography. The toxin was homogeneous in native and SDS-PAGE gel. SDS-molecular weight of the toxin was found to be 18.1 +/- 0.09 kDa. SA-HT produced severe haemorrhage on stomach wall but devoid of cutaneous haemorrhage. UV, EDTA, trypsin, protease, cyproheptadine, indomethacin, acetylsalicylic acid and BW755C treatment significantly antagonized the haemorrhagic activity of SA-HT. The toxin produced dose and time dependent oedema on mice hind paw, which was significantly encountered by cyproheptadine, indomethacin and BW755C. SA-HT increased capillary permeability on guinea pig dorsal flank. On isolated guineapig ileum, rat fundus and uterus, SA-HT produced slow contraction which was completely antagonised by prostaglandin blocker SC19220. On isolated rat duodenum, SA-HT produced slow relaxation. SA-HT significantly increased plasma plasmin, serum MDA level and decreased serum SOD level indicating the possible involvement of cyclooxygenase and lipooxygenase pathway.
Karmakar
S
S
Postgraduate Department of Zoology, Maulana Azad College, Kolkata 700 013, India.
Muhuri
D C
DC
Dasgupta
S C
SC
Nagchaudhuri
A K
AK
Gomes
A
A
D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D002196:Capillaries; D002852:Chromatography, Ion Exchange; D003533:Cyproheptadine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D005397:Fish Venoms / Q000737:chemistry* / Q000302:isolation & purification*; D005765:Gastrointestinal Agents / Q000494:pharmacology; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism; D051379:Mice; D009130:Muscle, Smooth / Q000187:drug effects; D010473:Perciformes; D010539:Permeability; D051381:Rats; D013131:Spine / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014466:Ultraviolet Rays; D014599:Uterus / Q000187:drug effects
D000894:Anti-Inflammatory Agents, Non-Steroidal; D029941:Fish Proteins; D005397:Fish Venoms; D005765:Gastrointestinal Agents; C488388:toxin SA-H, Scatophagus argus; D003533:Cyproheptadine; D015772:4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; D004492:Edetic Acid; D008084:Lipoxygenase; D013482:Superoxide Dismutase; D014357:Trypsin; D001241:Aspirin; D007213:Indomethacin