title: [Regulatory mechanism of EB1089 for hepatocarcinoma cell proliferation].
abstract: OBJECTIVE
  Study regulatory mechanism of EB1089 for hepatocarcinoma cell proliferation.
  
  
  METHODS
  HHCC were incubated in culture media with 100 nmol/L, 10 nmol/L and 1 nmol/L EB1089 for 2 d, 4 d and 6 d respectively. The anti-proliferative effect were examined by MTT and the plate clone forming test. The apoptosis was detected by Electron Microscope and flow cell device. Westernblot were used to detect p27Kipl and PTEN expression.
  
  
  RESULTS
  EB1089 could inhibit the proliferation of hepatocellular cell line HHCC. EB1089 could induce apoptosis of hepatocarcinoma cells. Western blot showed that EB1089 could elevate the expression level of p27Kipl and PTEN protein.
  
  
  CONCLUSIONS
  EB1089's inhibition to hepatocarcinoma is probably realized through inducing apoptosis of hepatocarcinoma cells and elevating the expression level of p27Kipl and PTEN protein.
authors.affiliation: Department of Occupational and Environmental Hygiene, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Luo
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Chen
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenliang
authors.initials: W
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D014807:Vitamin D / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D014807:Vitamin D; D002117:Calcitriol; C078903:seocalcitol