Long-size isoelectricfocusing (IEF) in flexible silicone tubes: an application to semi-preparative fractionation of proteins.
Soluble proteins extracted from porcine brains were subjected to a series of optional fractionation steps on various chromatographic media including a novel device for semi-preparative isoelectrofocusing (IEF) carried in a flexible silicone tube. The dimensions of the IEF granulated gel beds can be varied from 40 to 75 cm (length) and 0.4-1.6 cm (diameter) which are dependent on the protein's concentration. An average optimal focalisation time of proteins is dependent of the tube length, its diameter and complexity of proteins' mixtures but it is usually reached during 15,000-30,000 Vh. A series of sequential protein's fractionation techniques including semi-preparative IEF carried in the flexible silicone tube with the following dimensions: 75 cm in length and 1.6 cm in diameter permitted for observation and partial characterisation of several proteins whose expression levels are specifically high in the brain.
Galat
Andrzej
A
Département d'Ingénierie et d'Etudes des Protéines, DSV/CEA, CE-Saclay, F-91191 Gif-sur-Yvette Cedex, France. GALAT@DSVIDF.CEA.FR
Thai
Robert
R
D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012828:Silicones / Q000737:chemistry*; D013552:Swine
D011506:Proteins; D012828:Silicones