Characterization of a novel activation antigen on porcine lymphocytes recognized by monoclonal antibody 5A6/8.
In this report, we describe the characterization of a novel activation antigen on porcine lymphocytes recognized by mAb 5A6/8. This antigen was detected on B and T cells 24 h after treatment with various stimuli. It was also found on alveolar macrophages, and at low levels on untreated monocytes. MAb 5A6/8 precipitated two bands of 45 and 50 kDa under non-reducing conditions, and of 22 and 28 kDa under reducing conditions. The cellular distribution, expression kinetics and/or molecular size of the 5A6/8 antigen differ from those of other known lymphocyte activation antigens. MAb 5A6/8 was able to inhibit lymphocyte proliferative responses driven by different stimuli, suggesting a role for this molecule in the events that lead to lymphocyte activation.
Doménech
Nieves
N
Laboratory of Investigation, Juan Canalejo Medical Center, Xubias de Arriba, 84, 15006 La Coruña, Spain. ndomenech@canalejo.org
Bullido
Rosario
R
Alvarez
Belén
B
Campos
Emilia
E
Rodríguez-Carreño
María-Pilar
MP
Gómez Del Moral
Manuel
M
Alonso
Fernando
F
Ezquerra
Angel
A
Domínguez
Javier
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013552:Swine
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015375:Receptors, Interleukin-2