title: Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity.
abstract: We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego. 5A, 02106 Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kuban
authors.forename: Piotr
authors.initials: P
authors.lastname: Jonczyk
authors.forename: Damian
authors.initials: D
authors.lastname: Gawel
authors.forename: Karolina
authors.initials: K
authors.lastname: Malanowska
authors.forename: Roel M
authors.initials: RM
authors.lastname: Schaaper
authors.forename: Iwona J
authors.initials: IJ
authors.lastname: Fijalkowska
mesh_terms: D002876:Chromosomes, Bacterial / Q000378:metabolism*; D019951:DNA Polymerase beta / Q000378:metabolism*; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D017353:Gene Deletion; D017930:Genes, Reporter; D009154:Mutation; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; C094399:DinB protein, E coli; D029968:Escherichia coli Proteins; D019951:DNA Polymerase beta; D001616:beta-Galactosidase