title: The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class A penicillin-binding protein.
abstract: A soluble derivative of the Enterococcus faecalis JH2-2 class A PBP1 (*PBP1) was overproduced and purified. It exhibited a glycosyltransferase activity on the Escherichia coli 14C-labeled lipid II precursor. As a DD- peptidase, it could hydrolyze thiolester substrates with efficiencies similar to those of other class A penicillin-binding proteins (PBPs) and bind beta-lactams, but with k2/K (a parameter accounting for the acylation step efficiency) values characteristic of penicillin-resistant PBPs.
authors.affiliation: Centre d'Ingénierie des Protéines, Institut de Chimie, B6, Université de Liege, B-4000 Sart Tilman, Belgium. jcoyette@ulg.ac.be
authors.forename: Colette
authors.initials: C
authors.lastname: Duez
authors.forename: Séverine
authors.initials: S
authors.lastname: Hallut
authors.forename: Noureddine
authors.initials: N
authors.lastname: Rhazi
authors.forename: Séverine
authors.initials: S
authors.lastname: Hubert
authors.forename: Ana
authors.initials: A
authors.lastname: Amoroso
authors.forename: Fabrice
authors.initials: F
authors.lastname: Bouillenne
authors.forename: André
authors.initials: A
authors.lastname: Piette
authors.forename: Jacques
authors.initials: J
authors.lastname: Coyette
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics*; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D016695:Glycosyltransferases