[Study on the association of PPP1R3 gene polymorphism with type 2 diabetes in Han population of Anhui province].
OBJECTIVE
To study the association of muscle-specific glycogen-targeting regulatory subunit of the glucogen-bound protein phosphatase 1 (PPP1R3) gene codon 905 Asp/Tyr polymorphism with type 2 diabetes in Chinese Han population in Hefei region of Anhui province.


METHODS
PPP1R3 gene Asp905Tyr polymorphism was detected by polymerase chain reaction and appropriate restriction enzyme (PCR-RFLP) in 262 type 2 diabetic cases and 104 normal controls. Case and control groups were divided into subgroups by body mass index (BMI) 25 kg/m2.


RESULTS
When PPP1R3 gene Asp905Tyr polymorphism was not associated with type 2 diabetes mellitus. When subjects with BMI < 25 kg/m2 and Tyr/Tyr genotypes were used as reference. Subjects with Asp905 and BMI > or = 25 kg/m2 had a 3.69-fold increase of risk suffering from type 2 diabetes (OR = 3.69, 95% CI: 1.38-8.89, P=0.006).


CONCLUSIONS
PPP1R3 gene Asp905Tyr polymorphism did not seem to play a critical role in the development of type 2 diabetes mellitus in Han population of Chinese in Anhui province but interaction between the Asp905 and BMI cause the increase of risk of type 2 diabetes.
Chen
Ming-Wei
MW
Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Yang
Ming-Gong
MG
Wang
Chang-Jiang
CJ
Wang
You-Min
YM
Xu
Xi-Ping
XP
Liu
Shu-Qin
SQ
Zhang
Qiu
Q
Sun
Hai-Yan
HY
D000328:Adult; D000483:Alleles; D001224:Aspartic Acid / Q000235:genetics*; D002681:China / Q000453:epidemiology / Q000208:ethnology; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications*; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D054645:Protein Phosphatase 1; D012307:Risk Factors; D014443:Tyrosine / Q000235:genetics*
D001224:Aspartic Acid; D014443:Tyrosine; C114205:PPP1R3A protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1