A method for diagnosis of plant environmental stresses by gene expression profiling using a cDNA macroarray.
Plants in the field are subjected to numerous environmental stresses. Lengthy continuation of such environmental stresses or a rapid increase in their intensity is harmful to vegetation. Assessments of the phytotoxicity of various stresses have been performed in many countries, although they have largely been based on estimates of leaf injury. We developed a novel method of detecting plant stresses that is more sensitive and specific than those previously available. This method is based on the detection of mRNA expression changes in 205 ozone-responsive Arabidopsis expressed sequence tags (ESTs) by cDNA macroarray analysis. By using this method, we illustrated shifts in gene expression in response to stressors such as drought, salinity, UV-B, low temperature, high temperature, and acid rain, as distinct from those in response to ozone. We also made a mini-scale macroarray with 12 ESTs for diagnosis of the above environmental stresses in plants. These results illustrate the potential of our cDNA macroarray for diagnosis of various stresses in plants.
Tamaoki
Masanori
M
Biodiversity Conservation Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. mtamaoki@nies.go.jp
Matsuyama
Takashi
T
Nakajima
Nobuyoshi
N
Aono
Mitsuko
M
Kubo
Akihiro
A
Saji
Hikaru
H
D000397:Air Pollution; D017360:Arabidopsis / Q000235:genetics*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010126:Ozone; D043922:Toxicogenetics; D014466:Ultraviolet Rays
D010126:Ozone