[Accumulation of heat-shock proteins 70 in insect fat bodies as evidence of stress in Microsporidia-infected insects].
At present a concept prevails that pathological alterations in insect hosts infected with microsporidia, and those associated with hormone imbalance may be explained by the production of juvenile hormone-like (JH) substances by microsporidia. According to another view point, this pathology is a consequence of the host response. We suggested that the microsporidian infection can provoke a stress reaction in insects, which may cause JH secretion by these insects. To confirm this hypothesis, we have analysed major stress protein Hsp70 levels in the infected insects. Using affinity chromatography on ATP-agarose and immunoblotting, we have shown that Hsp70 was accumulated in infected crickets, and that it was the host protein. The consequence of events accompanying the infection in the insects is discussed in relation to the response of hormonal system of the host organism.
Seleznev
K V
KV
Institute of Cytology RAS, St. Petersburg.
Kinev
A V
AV
Margulis
B A
BA
D000818:Animals; D002846:Chromatography, Affinity; D003335:Corpora Allata / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism* / Q000469:parasitology; D006135:Gryllidae / Q000033:anatomy & histology / Q000469:parasitology* / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000378:metabolism*; D006458:Hemolymph / Q000737:chemistry; D006790:Host-Parasite Interactions; D007605:Juvenile Hormones / Q000378:metabolism; D016814:Microsporidia / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure
D018840:HSP70 Heat-Shock Proteins; D007605:Juvenile Hormones