Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity.
We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.
Kuban
Wojciech
W
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego. 5A, 02106 Warsaw, Poland.
Jonczyk
Piotr
P
Gawel
Damian
D
Malanowska
Karolina
K
Schaaper
Roel M
RM
Fijalkowska
Iwona J
IJ
D002876:Chromosomes, Bacterial / Q000378:metabolism*; D019951:DNA Polymerase beta / Q000378:metabolism*; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D017353:Gene Deletion; D017930:Genes, Reporter; D009154:Mutation; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004269:DNA, Bacterial; C094399:DinB protein, E coli; D029968:Escherichia coli Proteins; D019951:DNA Polymerase beta; D001616:beta-Galactosidase