Effects of isothiocyanates on tumor necrosis factor-alpha production by J774A.1 (BALB/c macrophage) cells.
The effects of isothiocyanates (ITCs) on tumor necrosis factor-alpha (TNF-alpha) production by the J774A.1 mouse macrophage cell line stimulated with lipopolysaccharide (LPS) were examined. Some individual ITCs examined showed a priming effect, which was expressed as pre-activation, but also inhibited the production of TNF-alpha with concomitant stimulation by LPS, as a triggering stimulant of TNF production. These results suggest that ITCs exert opposing activities that can enhance or inhibit the host defense system.
Ohta
Yoshio
Y
Hiroshima Prefectural Food Technology Research Center, Hiroshima, Japan.
Yamaki
Kohji
K
Osawa
Toshihiko
T
Kawakishi
Shunro
S
D000818:Animals; D002478:Cells, Cultured; D004336:Drug Antagonism; D017879:Isothiocyanates / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
D017879:Isothiocyanates; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha