title: Differential expression and localization of decorin in human choriodecidual membrane during preterm and term pregnancy.
abstract: OBJECTIVE
  This study was aimed to isolate cDNAs for genes whose expression levels are dynamically regulated at the feto-maternal interface around parturition.
  
  
  METHODS
  The suppression subtractive hybridization (SSH) method was used to compare the gene expression patterns of human choriodecidual membranes from term elective Caesarian delivery before labor (BL) and term vaginal delivery (VD). Northern blotting, quantitative real-time RT-PCR and in situ hybridization were performed to determine temporal and spatial expression of candidate clones.
  
  
  RESULTS
  After SSH procedure, we selected decorin from the cDNA library of BL for further investigation. The mean level of decorin transcription tended to be higher in BL samples than in VD or preterm samples. Decorin mRNA was mainly expressed in the decidual cells. Decorin gene expression was also upregulated in the term myometrium.
  
  
  CONCLUSIONS
  These results suggest that dynamically increased expression of decorin in the choriodecidual membrane during parturition may be a part of the uterine preparation for labor.
authors.affiliation: Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Suita, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Ogita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Koyama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsujie
authors.forename: Mari
authors.initials: M
authors.lastname: Tomiie
authors.forename: Tateki
authors.initials: T
authors.lastname: Tsutsui
authors.forename: Koichiro
authors.initials: K
authors.lastname: Shimoya
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Wada
authors.forename: Masayasu
authors.initials: M
authors.lastname: Koyama
authors.forename: Shintaro
authors.initials: S
authors.lastname: Nomura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Murata
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D007743:Labor, Obstetric / Q000235:genetics / Q000502:physiology; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
chemical_list: C546878:DCN protein, human; D018076:DNA, Complementary; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger