title: Risk factors for carcinoma of the pelvic ileal pouch/anal canal in ulcerative colitis.
abstract: Patients with ulcerative colitis who undergo proctocolectomy and an ileal anal anastomosis (IPAA) require surveillance; dysplasia and carcinoma occur in both the small intestinal mucosa of the ileal pouch and the retained rectal mucosa as early as 2 yr after ileostomy closure. This study evaluated risk factors for carcinoma (eg, dysplasia, p53 overexpression, labeling index, and aneuploidy) in the small intestinal and rectal mucosa. Thirty patients (age 14-64 yr) with ulcerative colitis and IPAA were studied. The mean duration of ulcerative colitis prior to IPAA was 3 yr (range 6 mo-21 yr). Patients were followed by annual endoscopy and biopsies of the ileal pouch and rectal mucosa. Sections of small intestine and rectal mucosa were evaluated for inflammation and dysplasia, and by immunohistochemical stains Ki-67 (MIB-1) for a labeling index and for p53. Ploidy determination was performed by flow cytometry. Active inflammation of the small intestinal mucosa and the rectal mucosa was frequent and the labeling index of both the pouch and rectal mucosa was abnormal. Two patients had changes indefinite for dysplasia, one involving the small bowel mucosa of the pouch and the other the retained rectal mucosa. Fifteen of the 30 patients had overexpression of p53, 9 from the pouch, and 6 from the rectal mucosa. Overexpression of p53 was seen in both of the patients with indefinite dysplasia. Aneuploidy was noted in 3 patients: two from the pouch and one from the rectal mucosa. All aneuploidic specimens were p53-positive, but negative for dysplasia. In conclusion, most biopsies of the ileal pouch and rectal mucosa were inflamed. The labeling indexes of the small bowel and rectal mucosa were higher than normal. The risk factors for carcinoma (dysplasia, overexpression of p53, and aneuploidy) occurred in the small intestinal and the rectal mucosa. Overexpression of p53 was noted in 16 patients, dysplasia only in 2. Therefore, p53 overexpression and aneuploidy should be considered in the evaluation of surveillance biopsies of patients with ulcerative colitis with IPAA, whereas dysplasia is an insensitive marker.
authors.affiliation: Department of Pathology, North Shore University Hospital, New York University School of Medicine, Manhasset, New York 11030, USA.
authors.forename: David
authors.initials: D
authors.lastname: Elkowitz
authors.forename: Fredric
authors.initials: F
authors.lastname: Daum
authors.forename: James
authors.initials: J
authors.lastname: Markowitz
authors.forename: John
authors.initials: J
authors.lastname: Proccaccino
authors.forename: Eleonore
authors.initials: E
authors.lastname: Boas
authors.forename: Joanne
authors.initials: J
authors.lastname: Cuomo
authors.forename: Ellen
authors.initials: E
authors.lastname: Kahn
mesh_terms: D000293:Adolescent; D000328:Adult; D001003:Anal Canal / Q000378:metabolism / Q000473:pathology*; D002277:Carcinoma / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D003093:Colitis, Ulcerative / Q000150:complications* / Q000175:diagnosis / Q000601:surgery; D039021:Colonic Pouches / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010388:Pelvis; D012307:Risk Factors