Low concentration of oxidant and nitric oxide donors stimulate proliferation of human endothelial cells in vitro.
Proliferation of human umbilical vein endothelial cells in vitro was inhibited by high concentrations of oxidants and nitric oxide donors but stimulated by low (micromolar or submicromolar) concentrations of hydrogen peroxide, menadione, tert-butyl hydroperoxide, AAPH, nitroglycerin, SIN-1 and sodium nitroprusside. The stimulation seems to be dependent upon generation of secondary reactive oxygen species as inferred from attenuation of cell proliferation by superoxide dismutase and catalase. These results point to another type of possible artefact of cell culture, viz. stimulation of cell proliferation by low concentrations of oxidants.
Łuczak
Katarzyna
K
Department of Molecular Biophysics, University of Łódź, Banacha 12/13, 90-237 Lodz, Poland.
Balcerczyk
Aneta
A
Soszyński
Mirosław
M
Bartosz
Grzegorz
G
D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005562:Formazans / Q000494:pharmacology*; D006801:Humans; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000494:pharmacology*
D005562:Formazans; D020030:Nitric Oxide Donors; D016877:Oxidants; D013778:Tetrazolium Salts; C079782:MTT formazan