title: 1H and 13C HR-MAS spectroscopy of intact biopsy samples ex vivo and in vivo 1H MRS study of human high grade gliomas.
abstract: High-resolution magic angle spinning (HR-MAS) one- and two-dimensional 1H and 13C nuclear magnetic resonance (NMR) spectroscopy has been used to study intact glioblastoma (GBM) brain tumour tissue. The results were compared with in vitro chemical extract and in vivo spectra. The resolution of 1H one-dimensional, 1H TOCSY and 13C HSQC HR-MAS spectra is comparable to that obtained on perchloric extracts. 13C HSQC HR-MAS spectra have been particularly useful for the identification of 37 different metabolites in intact biopsy tumours, excluding water and DSS components. To our knowledge, this is the most detailed assignment of biochemical compounds obtained in intact human tissue, in particular in brain tumour tissue. Tissue degradation during the recording of the NMR experiment was avoided by keeping the sample at a temperature of 4 degrees C. Detailed metabolical compositions of 10 GBM (six primary, two secondary and two unclassified) were obtained. A good correlation between ex vivo and in vivo MRS has been found.
authors.affiliation: Departamento de Química-Física, Universitat de Valencia, Valencia, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez-Bisbal
authors.forename: Luis
authors.initials: L
authors.lastname: Martí-Bonmatí
authors.forename: José
authors.initials: J
authors.lastname: Piquer
authors.forename: Antonio
authors.initials: A
authors.lastname: Revert
authors.forename: Pilar
authors.initials: P
authors.lastname: Ferrer
authors.forename: José L
authors.initials: JL
authors.lastname: Llácer
authors.forename: Martial
authors.initials: M
authors.lastname: Piotto
authors.forename: Olivier
authors.initials: O
authors.lastname: Assemat
authors.forename: Bernardo
authors.initials: B
authors.lastname: Celda
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D002247:Carbon Isotopes; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis* / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D002247:Carbon Isotopes; D009363:Neoplasm Proteins; D011522:Protons