Low transposition frequency of the medaka fish Tol2 element may be due to extranuclear localization of its transposase.
Transposase proteins of some highly active DNA-based transposable elements, such as the maize Activator element, are known to possess nuclear localization signals (NLSs). We examined if this is also the case for the transposase of the medaka fish Tol2 element, a member of the hAT (hobo/Activator/Tam3) transposable element family, using human and mouse culture cells. Unexpectedly, the transposase-lacZ fusion protein, in which the lacZ is a location marker, was found to be present in the cytoplasm rather than in the nucleus, suggesting that the Tol2 transposase contains a signal for extranuclear localization. The same staining pattern was also observed with a fusion protein containing a 33-amino-acid region at about the center of the primary structure of the transposase. The Tol2 element might have a mechanism to control its transposition frequency that includes extranuclear localization of its transposase.
Iida
Atsuo
A
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
Tachibana
Akira
A
Hamada
Susumu
S
Hori
Hiroshi
H
Koga
Akihiko
A
D000818:Animals; D004251:DNA Transposable Elements; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009990:Oryzias / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019895:Transposases / Q000235:genetics*
D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D019895:Transposases