[Muscle regeneration following glycerol injection mimic that of mdx-mice degenerative-regenerative groups].
BACKGROUND
In recent years several authors have proposed that muscle pathological changes in humans with dystrophin disorders and in mdx-mice are the result of regeneration attempts of this tissue subjected to constantly repeated bouts of necrosis.


OBJECTIVE
We compared morphological aspects of degenerative-regenerative groups observed in the mdx-mice with the regenerative phases that follow an intramuscular injection of 30 mL glycerol.


METHODS
Gastrocnemius samples from mdx-mice, 1 to 5 months old and from glycerol injected C57Bl10/ScSn mice, 4 to 8 months old, were processed and analyzed in parallel.


RESULTS
We were able to recognize degenerative-regenerative group stages in the mdx-mice that closely matched the anomalies found 1, 2, 3, 4-5, 7 and 21-28 days following the injection with glycerol. CONCLUSION. This study reinforces the hypothesis that muscle pathologic abnormalities of mdx-mice are the result of chronic muscle necrosis-regeneration. Morphologic mdx-mice degenerative-regenerative group staging will be a helpful tool for future investigation of muscle regeneration.
Roig-Quilis
M
M
Grup de Recerca de Malalties Neurometabòliques, Hospital Universitari Materno-Infantil, Hospital Universitari Vall d'Hebron, Barcelona, Spain. manroig@cs.vhebron.es
Roma
J
J
Marotta
M
M
Sarria
Y
Y
Fargas
A
A
D000818:Animals; D005260:Female; D005990:Glycerol / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000473:pathology* / Q000502:physiology; D009137:Muscular Dystrophy, Animal / Q000188:drug therapy / Q000473:pathology; D012038:Regeneration / Q000502:physiology*
D005990:Glycerol