Novel cell patterning using microheater-controlled thermoresponsive plasma films.
A novel approach is reported for cell patterning based on addressable microheaters and a poly(N-isopropyl acrylamide) (pNIPAM) themoresponsive coating. This thermoresponsive coating is created by a radio frequency NIPAM plasma and is denoted as plasma polymerized NIPAM (ppNIPAM). Films of ppNIPAM with a good retention of monomer side-chain functionality are produced using low-power continuous plasma deposition. Cell adhesion and cell detachment tests indicate that the surface switches between adhesive and nonadhesive behaviors as a function of temperature. The use of a photolithographically fabricated microheater array allows the ppNIPAM transition to occur spatially under the control of individual heaters. This localized change in the surface adhesive behavior is used to direct site-specific cell attachment. Patterned adhesion of two types of cells has been visualized on the array through fluorescent markers. Applications for diagnostic devices, cell-based sensors, tissue engineering, and cell transfection are envisioned.
Cheng
Xuanhong
X
Bioengineering Department, University of Washington Engineered Biomaterials, Seattle, Washington 98195, USA.
Wang
Yanbing
Y
Hanein
Yael
Y
Böhringer
Karl F
KF
Ratner
Buddy D
BD
D000180:Acrylic Resins; D000818:Animals; D001672:Biocompatible Materials; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D013499:Surface Properties
D000180:Acrylic Resins; D001672:Biocompatible Materials; C052970:poly-N-isopropylacrylamide