Differential activation of interferon regulatory factors-3 and -7 by non-cytopathogenic and cytopathogenic bovine viral diarrhoea virus.
Non-cytopathogenic bovine viral diarrhoea virus (ncpBVDV) has previously been shown to inhibit the function of interferon regulatory factor-3 in cultured cells [J. Virol. 76 (2002) 8979]. In this study, we show that, like ncpBVDV, when cells were previously exposed to cytopathogenic BVDV (cpBVDV) the appearance of an IRF-3-DNA complex from nuclear extracts that can be induced by heterologous virus infection was not observed. Infection of cells with ncpBVDV or cpBVDV resulted in neither the translocation of IRF-7 from the cytoplasm to the nucleus of infected cells, nor an inhibition of its nuclear translocation in cells super-infected by Semliki Forest Virus. We conclude that cpBVDV and ncpBVDV both share the ability to inhibit the full function of IRF-3 but neither stimulate or block the nuclear uptake of IRF-7.
Baigent
Susan J
SJ
Compton Laboratory, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK.
Goodbourn
Stephen
S
McCauley
John W
JW
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000276:immunology; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000472:pathogenicity; D024202:Electrophoretic Mobility Shift Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; D016755:Proto-Oncogene Proteins c-jun / Q000276:immunology; D012672:Semliki forest virus / Q000276:immunology; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D014774:Virulence
D004268:DNA-Binding Proteins; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors