In vitro effects of metal ions on lipid peroxidation induced by alcohol in mice liver homogenate.
The study is aimed to estimate the effect of different heavy metals such as Hg2+, Cd2+, Mn2+, Cr3+, Ni2+, Se2O3(2-), As2O3 water solution and combined effects of Hg2+, Mn2+, Cr3+ on lipid peroxidation in mice liver homogenate in vitro. Lipid peroxidation was determined as thiobarbituric acid-reacting materials (TBA). We select five different concentrations of selected ions for experiments. Correlations used to identify the concentration of ions associated with lipid peroxidation. The rate of lipid peroxide formation in mice liver homogenate increased with the gradual addition of alcohol. When alcohol dose was up to 0.5 ml, the rate of lipid peroxide formation was greatest. At tested concentrations, the effects of metal ions on lipid peroxidation induced by alcohol were classified into three groups, and are as follows: (1) simulative, Hg2+. (2) inhibitory, Mn2+, Cr3+, Ni2+, Se2O3(2-). (3) ambiguous, Cd2+, As2O3 water solution. When Hg2+, Mn2+ and Cr3+ were added to the mice liver homogenate with alcohol at the same time, Hg2+, Mn2+ were the main agents for the rate of alcohol induced lipid peroxidation. The simulative effect of Hg2+ on lipid peroxidation induced by alcohol indicate that alcohol-drinkers will have further health risk when they are exposed in polluted regions than others, and Mn2+, Cr3+, Ni2+, Se2O3(2-) may act as free radical scavengers and preventive remedy for alcoholism in part. Furthermore, analysis of combined effect of Hg2+Mn2+ and Cr3+ provide us a new way to estimate the combined effect of multi-materials.
Li
Hairong
H
Laboratory of Environment and Human Health, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China. lihr@igsnrr.ac.cn
Hou
Shaofan
S
Wang
Wuyi
W
Yang
Linsheng
L
Li
Yonghua
Y
Tan
Jian'an
J
D000704:Analysis of Variance; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019216:Metals, Heavy / Q000633:toxicity*; D051379:Mice; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D019216:Metals, Heavy; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol