title: CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin.
abstract: Tetraspanins are thought to facilitate the formation of multiprotein complexes at cell surfaces, but evidence illuminating the biologic importance of this role is sparse. Tetraspanin CD151 forms very stable laminin-binding complexes with integrins alpha3beta1 and alpha6beta1 in kidney and alpha3beta1 and alpha6beta4 in skin. It is encoded by a gene at the same position on chromosome 11p15.5 as the MER2 blood group gene. We show that CD151 expresses the MER2 blood group antigen and is located on erythrocytes. We examined CD151 in 3 MER2-negative patients (2 are sibs) of Indian Jewish origin with end-stage kidney disease. In addition to hereditary nephritis the sibs have sensorineural deafness, pretibial epidermolysis bullosa, and beta-thalassemia minor. The 3 patients are homozygous for a single nucleotide insertion (G383) in exon 5 of CD151, causing a frameshift and premature stop signal at codon 140. The resultant truncated protein would lack its integrin-binding domain. We conclude that CD151 is essential for the proper assembly of the glomerular and tubular basement membrane in kidney, has functional significance in the skin, is probably a component of the inner ear, and could play a role in erythropoiesis.
authors.affiliation: Bristol Institute for Transfusion Sciences, Southmead Road, Bristol, BS10 5ND, United Kingdom.
authors.forename: Vanja
authors.initials: V
authors.lastname: Karamatic Crew
authors.forename: Nicholas
authors.initials: N
authors.lastname: Burton
authors.forename: Alexander
authors.initials: A
authors.lastname: Kagan
authors.forename: Carole A
authors.initials: CA
authors.lastname: Green
authors.forename: Cyril
authors.initials: C
authors.lastname: Levene
authors.forename: Frances
authors.initials: F
authors.lastname: Flinter
authors.forename: R Leo
authors.initials: RL
authors.lastname: Brady
authors.forename: Geoff
authors.initials: G
authors.lastname: Daniels
authors.forename: David J
authors.initials: DJ
authors.lastname: Anstee
mesh_terms: D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D001485:Basement Membrane / Q000378:metabolism* / Q000648:ultrastructure; D001706:Biopsy; D002478:Cells, Cultured; D004820:Epidermolysis Bullosa / Q000150:complications / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D009394:Nephritis, Hereditary / Q000150:complications / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017434:Protein Structure, Tertiary; D051437:Renal Insufficiency / Q000150:complications / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D060185:Tetraspanin 24
chemical_list: D015703:Antigens, CD; C554715:CD151 protein, human; D060185:Tetraspanin 24