Tissue expression of 165-aa vascular permeability factor after spinal cord injury is not influenced by dexamethasone administration in rats.
Using immunohistochemistry, RT-PCR, and Western Blot techniques, we studied the tissue expression of the 165-aa Vascular permeability factor (VPF) after spinal cord injury (SCI) in adult Wistar rats. The results were compared according to that the animals received or non-dexamethasone, at the dose of 1mg/kg and day after trauma. Furthermore, the different functional recovery between treated and non-treated animals was recorded. Although the administration of dexamethasone showed a beneficial effect on the functional recovery of the animals, the tissue expression of VPF after SCI is not influenced by dexamethasone administration. Therefore, the neuroprotective effect of the dexamethasone after experimental SCI is not mediated through an interference on the biological effects of the 165-aa vascular permeability factor.
Vaquero
Jesús
J
Department of Surgery, Neuroscience Research Unit of the Mapfre-Medicine Foundation, Neurosurgical Service, Puerta de Hierro Hospital, Autonomous University, San Martín de Porres 4, 28035 Madrid, Spain. jvaqueroc@telefonica.net
Zurita
Mercedes
M
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D007150:Immunohistochemistry; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D010264:Paraplegia / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013119:Spinal Cord Injuries / Q000150:complications / Q000188:drug therapy / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D018696:Neuroprotective Agents; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D003907:Dexamethasone