Multienzyme system for dihydroxyacetone phosphate-dependent aldolase catalyzed C-C bond formation from dihydroxyacetone.
A multienzyme system composed by recombinant dihydroxyacetone kinase from Citrobacter freundii, fuculose-1-phosphate aldolase and acetate kinase, allows a practical one-pot C-C bond formation catalysed by dihydroxyacetone phosphate-dependent aldolases from dihydroxyacetone and an aldehyde.
Sánchez-Moreno
Israel
I
Departamento de Química Orgánica Biológica, Instituto de Química Orgánica General, CSIC, Madrid 28006, Spain.
García-García
Juan Francisco
JF
Bastida
Agatha
A
García-Junceda
Eduardo
E
D002384:Catalysis; D004098:Dihydroxyacetone / Q000737:chemistry*; D004099:Dihydroxyacetone Phosphate / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics
D009097:Multienzyme Complexes; D004099:Dihydroxyacetone Phosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D005634:Fructose-Bisphosphate Aldolase; D004098:Dihydroxyacetone