title: Low-dose radiation modifies the progression of chronic renal failure.
abstract: Previous studies have indicated that the application of low dose radiation to an arterial ligation has the potential to subsequently reduce or eliminate restenosis caused by smooth muscle cell proliferation. Sufficient kidney irradiation causes a radiation nephropathy and often leads to renal failure. In order to evaluate the effect of low-dose irradiation on the kidney we hypothesized that this particular therapy modifies renal injury in rats with renal ablation and subsequently slows the rate of the progression. For further clarification of the effect of irradiation at low doses, we determined proliferating cell nuclear antigen (PCNA) and monocyte chemoattractant protein-1 (MCP-1) expression in remnant kidneys after low-dose radiation. Adult Wistar rats (n = 10) were studied during the two weeks after renal ablation. The left kidney was irradiated 24 hours after an operation in anaesthetised animals with 3 Grey in a single dose. Ablated rats without irradiation (n = 9) served as nephrectomized animals group. Rats without surgery and without radiation (n = 10) served as healthy controls. Renal damage was assessed using the following parameters: urine protein excretion rate (UprotV, mg/day), awake systolic blood pressure (SBP, mm Hg), serum creatinine (SCr, micromol/l). The indirect immunofluorescence method was used for the detection of PCNA and MCP-1 expression. Glomerular and tubular immunostaining was scored semiquantitatively. Numerous PCNA positive cells and MCP-1 expression were present in the glomerulus and tubulointerstitium in nephrectomized rat kidneys. Low-dose radiation application was associated with a significant reduction in PCNA and low MCP-1 expression. This study shows that the application of low-dose irradiation has the potential to modify the progression of chronic renal failure in rats.
authors.affiliation: Department of Anatomy, University of Tartu, Ravila 19, 51014 Tartu, Estonia. mariaun@ut.ee
authors.forename: Marina
authors.initials: M
authors.lastname: Aunapuu
authors.forename: Ulle
authors.initials: U
authors.lastname: Pechter
authors.forename: Eduard
authors.initials: E
authors.lastname: Gerskevits
authors.forename: Margit-Maie
authors.initials: MM
authors.lastname: Marjamägi
authors.forename: Sirje
authors.initials: S
authors.lastname: Suuroja
authors.forename: Andres
authors.initials: A
authors.lastname: Arend
authors.forename: Ivo
authors.initials: I
authors.lastname: Kolts
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kühnel
authors.forename: Mai
authors.initials: M
authors.lastname: Ots
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000528:radiation effects; D001835:Body Weight; D018932:Chemokine CCL2 / Q000032:analysis; D003404:Creatinine / Q000097:blood; D007668:Kidney / Q000033:anatomy & histology* / Q000528:radiation effects*; D007676:Kidney Failure, Chronic / Q000532:radiotherapy*; D009392:Nephrectomy; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018932:Chemokine CCL2; D018809:Proliferating Cell Nuclear Antigen; D003404:Creatinine