Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and Archaea in the deep ocean.
The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4',6'-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%).
Teira
Eva
E
Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. teira@nioz.nl
Reinthaler
Thomas
T
Pernthaler
Annelie
A
Pernthaler
Jakob
J
Herndl
Gerhard J
GJ
D001105:Archaea / Q000378:metabolism*; D001345:Autoradiography / Q000379:methods*; D001419:Bacteria / Q000378:metabolism*; D002384:Catalysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012623:Seawater / Q000382:microbiology*