Phosphorylcholine-carbohydrate-protein conjugates efficiently induce hapten-specific antibodies which recognize both Streptococcus pneumoniae and Neisseria meningitidis: a potential multitarget vaccine against respiratory infections.
Phosphorylcholine (ChoP) is commonly expressed at the surface of pathogens of the respiratory tract, including Streptococcus pneumoniae and Neisseria meningitidis. We designed a synthetic hapten comprising ChoP and part of its native carrier structure in S. pneumoniae, i.e. N-acetyl-D-galactosamine (GalNAc). Protein conjugates of this hapten induced GalNAc-ChoP-specific antibodies which recognized ChoP on both S. pneumoniae and N. meningitidis. GalNAc-ChoP could therefore lead to the rational design of a novel multipurpose vaccine against respiratory infections.
Bay
Sylvie
S
Unité de Chimie Organique URA CNRS 2128, Institut Pasteur, Paris, France. sbay@pasteur.fr
Huteau
Valérie
V
Zarantonelli
Maria-Leticia
ML
Pires
René
R
Ughetto-Monfrin
Joël
J
Taha
Muhamed-Kheir
MK
England
Patrick
P
Lafaye
Pierre
P
D000116:Acetylgalactosamine / Q000737:chemistry*; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000138:chemical synthesis / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D051379:Mice; D009345:Neisseria meningitidis / Q000276:immunology*; D010767:Phosphorylcholine / Q000737:chemistry*; D012141:Respiratory Tract Infections / Q000382:microbiology; D012709:Serum Albumin / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology*; D013744:Tetanus Toxin / Q000737:chemistry
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D006241:Haptens; D012709:Serum Albumin; D013744:Tetanus Toxin; D010767:Phosphorylcholine; D000116:Acetylgalactosamine