title: Stimulatory effects of monoacetyldiglycerides on hematopoiesis.
abstract: Monoacetyldiglycerides purified from deer antler, and identical synthetic compounds, have been shown to stimulate hematopoiesis. In the present study, we synthesized eight monoacetyldiglycerides, one of which, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (3), was structurally identical to one of the naturally occurring monoacetyldiglycerides and showed the most potent stimulation of hematopoiesis by colony forming unit in culture (CFUc) assay, having a stimulation index (SI) of 1.54+/-0.23 at a concentration of 1.0 microg/ml. Moreover, 1.0 microg/ml 3 showed potent growth-stimulation activities on megakaryocyte culture, long term culture of Lin(-)Sca-1(+) cells with irradiated MS-5 stromal cells (SI, 1.69+/-0.16), and on the number of cobblestone colonies (SI, 10.4+/-0.25). In a murine model, 3, at concentrations of 0.5, 5 and 50 mg/kg/d, i.p. and p.o., effectively stimulated hematopoiesis in vivo 7 d after syngenic bone marrow transplantation of irradiated C57BL/6 mice, when assayed by the colony forming units in spleen (CFUs) assay. These data suggest that monoacetyldiglycerides may have significant clinical potential for the acceleration of hematopoiesis.
authors.affiliation: Department of Anesthesiology & Pain Management, Asan Institute for Life Sciences, College of Medicine, University of Ulsan, Seoul, Korea.
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Yang
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: So-Hye
authors.initials: SH
authors.lastname: Cho
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Yoon
authors.forename: Mi-Geum
authors.initials: MG
authors.lastname: Kim
authors.forename: Gil-Ja
authors.initials: GJ
authors.lastname: Jhon
authors.forename: So-Yeop
authors.initials: SY
authors.lastname: Han
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000999:Antlers; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D003670:Deer; D004075:Diglycerides / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D004075:Diglycerides