Identification of biomarkers of whole Coxiella burnetii phase I by MALDI-TOF mass spectrometry.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) specific biomarkers have been shown to be an effective tool for identifying microorganisms. In this study, we demonstrate the feasibility of using this technique to detect the obligate intracellular bacterium Coxiella burnetii, a category B bioterrorism agent. Specific biomarkers were detected in C. burnetii Nine Mile phase I (NMI) strain purified from embryonated egg yolk sac preparations. Whole organisms were applied directly to the MALDI target. MALDI-TOF MS analysis of C. burnetii NMI grown and purified at different times and places revealed a group of unique, characteristic, and reproducible spectral markers in the mass range of 1000-25000 Da. Statistical analysis of the averaged centroided masses uncovered at least 24 peptides or biomarkers. Three biomarkers observed in the MALDI-TOF MS spectrum consistently matched proteins that had been previously described in C. burnetii, one of them being the small cell variant protein A. MALDI-TOF MS analysis of whole organisms represents a sensitive and specific option for characterizing C. burnetii isolates, especially when coupled with antigen capture techniques. The method also has potential for several applications in basic microbial research, including regulation of gene expression.
Shaw
Edward I
EI
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. eps0@cdc.gov
Moura
Hercules
H
Woolfitt
Adrian R
AR
Ospina
Maria
M
Thompson
Herbert A
HA
Barr
John R
JR
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002642:Chick Embryo; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004531:Eggs / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001426:Bacterial Proteins; D015415:Biomarkers