Gene structure and molecular analysis of Arabidopsis thaliana ALWAYS EARLY homologs.
Drosophila always early (aly) is essential for spermatogenesis, and is related to the LIN-9 protein of Caenorhabditis elegans; lin-9 is a class B Synthetic Multivulva gene (synMuvB) required for gonadal sheath development. Aly/LIN-9 have two conserved regions, called domains 1 and 2, which have been identified in homologous proteins from several multicellular eukaryotes, including the model plant Arabidopsis thaliana. We cloned and sequenced cDNAs of three different A. thaliana ALWAYS EARLY homologs (AtALY1, AtALY2 and AtALY3), analysed the expression pattern of these three genes and show that AtALY1, like Aly, is nuclear localised. We also demonstrate that the plant homologs of aly/lin-9 contain an additional N-terminal myb domain not present in the animal Aly/LIN-9 proteins, and that part of the ALY/LIN-9 conserved domain 1 in the predicted plant proteins is related to the TUDOR domain.
Bhatt
Anuj M
AM
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK. Anuj.Bhatt@plants.ox.ac.uk
Zhang
Qing
Q
Harris
Stephen A
SA
White-Cooper
Helen
H
Dickinson
Hugh
H
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016385:TATA Box / Q000235:genetics; D014026:Nicotiana / Q000166:cytology
C492086:ALY1 protein, Arabidopsis; C492087:ALY2 protein, Arabidopsis; C492088:ALY3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins