title: Osmotic stress response: quantification of cell maintenance and metabolic fluxes in a lysine-overproducing strain of Corynebacterium glutamicum.
abstract: Osmotic stress diminishes cell productivity and may cause cell inactivation in industrial fermentations. The quantification of metabolic changes under such conditions is fundamental for understanding and describing microbial behavior during bioprocesses. We quantified the gradual changes that take place when a lysine-overproducing strain of Corynebacterium glutamicum is grown in continuous culture with saline gradients at different dilution rates. The use of compatible solutes depended on environmental conditions; certain osmolites predominated at different dilution rates and extracellular osmolalities. A metabolic flux analysis showed that at high dilution rates C. glutamicum redistributed its metabolic fluxes, favoring energy formation over growth. At low dilution rates, cell metabolism accelerated as the osmolality was steadily increased. Flexibility in the oxaloacetate node proved to be key for the energetic redistribution that occurred when cells were grown at high dilution rates. Substrate and ATP maintenance coefficients increased 30- and 5-fold, respectively, when the osmolality increased, which demonstrates that energy pool management is fundamental for sustaining viability.
authors.affiliation: Departamento de Ingeniería Química y Bioprocesos, Escuela de Ingeniería, Pontificia Universidad Católica de Chile, Casilla 306, Correo 22, Santiago 782-0436 M, Chile.
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Varela
authors.forename: Mauricio E
authors.initials: ME
authors.lastname: Baez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Agosin
mesh_terms: D018533:Biomass; D003352:Corynebacterium / Q000378:metabolism*; D004734:Energy Metabolism; D008239:Lysine / Q000378:metabolism*; D009994:Osmolar Concentration; D014199:Trehalose / Q000378:metabolism
chemical_list: D014199:Trehalose; D008239:Lysine