Extracellular nucleic acids in cultures of long-term cultivated eukaryotic cells.
Investigation of the kinetics of nucleic acid release by HeLa (human cervical carcinoma cell line) and A431 (human squamous carcinoma cell line) cells is presented. The released DNA and RNA were shown to accumulate in culture medium and at the cell surface. A portion of cell surface bound RNA can be eluted with PBS/EDTA. Mild trypsin treatment is required for complete detachment of cell surface bound RNA and cell surface bound DNA. Electrophoretic analysis reveals characteristic patterns of cell-associated and free RNA and DNA molecules.
Morozkin
Evgeniy S
ES
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Laktionov
Pavel P
PP
Rykova
Elena Y
EY
Vlassov
Valentin V
VV
D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D004247:DNA / Q000032:analysis; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004847:Epithelial Cells / Q000187:drug effects; D005057:Eukaryotic Cells / Q000737:chemistry* / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D002614:Chelating Agents; D003470:Culture Media; D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D014357:Trypsin