title: The decidual suction method: a new way of collecting decidual tissue for functional and morphological studies.
abstract: BACKGROUND
  Studies of extravillous trophoblasts and placental bed spiral arteries are essential for a better understanding of pathological pregnancies such as preeclampsia, intrauterine growth restriction and diabetes mellitus. A major challenge is to obtain representative and sufficient tissue for morphological and functional investigations. Currently, tissue material is mostly harvested by placental bed biopsy (PBB). We describe a new suction method to obtain a larger volume of decidual tissue from the placental bed.
  
  
  METHODS
  Tissue was harvested in 51 cesarean sections by vacuum suction of the placental bed. Sections from formalin-fixed, paraffin-embedded tissue were routinely stained with hematoxylin and eosin (H&E), immunostained with a panel of antibodies and morphologically examined for the presence of trophoblasts and spiral arteries. The results were compared with those from archive material from PBBs and placental basal plate sections (BPSs). Short-term adverse events were registered for the study patients. Long-term complications were registered from medical charts of 151 women having undergone the decidual suction method (DSM), with a follow-up of 38-60 months.
  
  
  RESULTS
  In 86% (n = 44), one random section from the decidual suction material demonstrated at least one spiral artery. In 37% (n = 19), six or more spiral arteries were present. All sections revealed extravillous trophoblasts. No short- or long-term morbidity was recorded.
  
  
  CONCLUSIONS
  The decidual suction method represents an important improvement in the work to obtain sufficient decidual tissue for morphological and functional studies of extravillous trophoblast function and spiral artery adaptation. The method is safe, as no short- or long-term complications were registered.
authors.affiliation: Department of Gynecology and Obstetrics, Ullevål University Hospital, Oslo, Norway. mailto:nina.harsem@ulleval.no
authors.forename: Nina Kittelsen
authors.initials: NK
authors.lastname: Harsem
authors.forename: Anne Cathrine
authors.initials: AC
authors.lastname: Staff
authors.forename: Liping
authors.initials: L
authors.lastname: He
authors.forename: Borghild
authors.initials: B
authors.lastname: Roald
mesh_terms: D003656:Decidua / Q000098:blood supply / Q000473:pathology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis* / Q000473:pathology; D011296:Prenatal Diagnosis / Q000379:methods*; D013396:Suction / Q000379:methods*