Chronic lymphocytic leukemia in the course of chronic myelocytic leukemia: evidence of independent clonal origin as shown by interphase fluorescence in situ hybridization and fluorescence-activated cell sorting.
We report the case of a 57-year-old man who developed chronic lymphocytic leukemia (CLL) several months after the initial diagnosis of Philadelphia (Ph) chromosome-positive (Ph(+)) chronic myelocytic leukemia. CLL cells were purified by using fluorescence-activated cell sorting and further analyzed using interphase fluorescence in situ hybridization with probes to detect the BCR/ABL fusion gene. We provide evidence that the CLL cells arose in a Ph(-) clone.
Chang
Hong
H
Department of Laboratory Hematology, Princess Margaret Hospital-University Health Network, University of Toronto, 610 University Ave., Toronto, ON M5G 2M9, Canada. hong.chang@uhn.on.ca
Sutherland
Rob
R
Nayar
Rakash
R
Li
Dan
D
Kamel-Reid
Suzane
S
Mile
Mary-Anne
MA
Messner
Hans
H
Lipton
Jeff
J
D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl / Q000032:analysis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D054438:Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome
D016044:Fusion Proteins, bcr-abl