title: In vitro anti-coxsackievirus B(3) effect of ethyl acetate extract of Tian-hua-fen.
abstract: OBJECTIVE
  To investigation the anti-coxsackievirus B(3) (CVB(3m)) effect of the ethyl acetate extract of Tian-hua-fen on HeLa cells infected with CVB(3m).
  
  
  METHODS
  HeLa cells were infected with CVB(3m) and the cytopathic effects (CPE) were observed through light microscope and crystal violet staining on 96-well plate and A(600) was detected using spectrophotometer. The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A(600).
  
  
  RESULTS
  The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration. Consistent results were got through these two methods. We also investigated the mechanism of its anti-CVB(3m) effect and the results indicated that the extract represented an inhibitory effect through all the processes of CVB(3m) attachment, entry, biosynthesis and assemble in cells.
  
  
  CONCLUSIONS
  The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significant protective effect on HeLa cells infected with CVB(3m) in a dose-dependent manner and this effect exists through the process of CVB(3m) attachment, entry, biosynthesis and assemble in cells, suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.
authors.affiliation: Department of Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Zhen-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Nie
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Guo
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Liu
mesh_terms: D000085:Acetates; D000998:Antiviral Agents / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D015978:Trichosanthin / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000998:Antiviral Agents; D010936:Plant Extracts; D015978:Trichosanthin; C007650:ethyl acetate