title: Human skeletal muscle lactate dehydrogenase activity in the presence of some alcohol dehydrogenase inhibitors.
abstract: Methanol, ethylene glycol and other alcohol intoxications are complicated by severe acidosis which could be caused by formation of metabolic acids and additionally lactic acid production. An increasing nicotinamide adenine dinucleotide reduced/nicotinamide adenine dinucleotide oxidized (NADH/NAD) ratio during alcohol biotransformation is responsible for the induction of lactic acidosis. The main purpose of the present paper was to evaluate the effect of 4-methylpyrazole, cimetidine, ethylenediaminetetraacetic acid disodium salt, ethanol and methanol on lactate dehydrogenase (E.C. 1.1.1.27) activity and to discuss this issue. The activity of the enzyme was determined spectrophotometrically, in vitro using human enzyme skeletal muscle homogenates. 4-Methylpyrazole, cimetidine and ethylenediaminetetraacetic acid disodium salt at concentrations 0.01, 0.1, 1.0 mM and 12.5, 25.0, 50.0 mM of ethanol and methanol were studied. Our results showed that cimetidine increased lactate dehydrogenase activity as compared to the control at all tested concentrations. Such activity was noted for 4-methylpyrazole at 0.1 mM and higher concentration. By contrast, no significant effect on lactate dehydrogenase activity in the presence of ethylenediaminetetraacetic acid disodium salt, methanol and ethanol was observed.
authors.affiliation: Department of Clinical Pathomorphology, Medical University of Lublin, 8 Jaczewskiego Str., PL 20-090, Poland. ave123@wp.pl
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Dudka
authors.forename: Franciszek
authors.initials: F
authors.lastname: Burdan
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korobowicz
authors.forename: Robert
authors.initials: R
authors.lastname: Klepacz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Korobowicz
mesh_terms: D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors*; D002927:Cimetidine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D000077604:Fomepizole; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D000432:Methanol / Q000494:pharmacology; D010369:Pectoralis Muscles / Q000187:drug effects* / Q000201:enzymology; D011720:Pyrazoles / Q000494:pharmacology
chemical_list: D011720:Pyrazoles; D000431:Ethanol; D002927:Cimetidine; D000077604:Fomepizole; D004492:Edetic Acid; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D000432:Methanol