title: Founder myoblasts and fibre number during adult myogenesis in Drosophila.
abstract: We have examined the mechanisms underlying the setting of myotubes and choice of myotube number in adult Drosophila. We find that the pattern of adult myotubes is prefigured by a pattern of duf-lacZ-expressing myoblasts at appropriate locations. Selective expression of duf-lacZ in single myoblasts emerges from generalized, low-level expression in all adult myoblasts during the third larval instar. The number of founders, thus chosen, corresponds to the number of fibres in a muscle. In contrast to the embryo, the selection of individual adult founder cells during myogenesis does not depend on Notch-mediated lateral inhibition. Our results suggest a general mechanism by which multi-fibre muscles can be patterned.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India.
authors.forename: Devkanya
authors.initials: D
authors.lastname: Dutta
authors.forename: Sumita
authors.initials: S
authors.lastname: Anant
authors.forename: Mar
authors.initials: M
authors.lastname: Ruiz-Gomez
authors.forename: Michael
authors.initials: M
authors.lastname: Bate
authors.forename: K
authors.initials: K
authors.lastname: VijayRaghavan
mesh_terms: D000818:Animals; D019521:Body Patterning; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D007814:Larva / Q000166:cytology / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000502:physiology; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C497759:N protein, Drosophila; C090146:Rac1 protein, Drosophila; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; C413082:kirre protein, Drosophila; D020744:rac GTP-Binding Proteins