Multiple gene differential expression patterns in human ischemic liver: safe limit of warm ischemic time.
OBJECTIVE
To investigate the multiple gene differential expression patterns in human ischemic liver and to produce the evidence about the hepatic ischemic safety time.


METHODS
The responses of cells to hepatic ischemia and hypoxia at hepatic ischemia were analyzed by cDNA microarrary representing 4 000 different human genes containing 200 apoptotic correlative genes.


RESULTS
There were lower or normal expression levels of apoptotic correlative genes during the periods of hepatic ischemia for 0-15 min, the maintenance homostatic genes were expressed significantly higher at the same time. But at the hepatic ischemia for 30 min, the expression levels of maintenance homeostatic genes were down-regulated, the expressions of many apoptotic correlative genes and nuclear transcription factors were activated and up-regulated.


CONCLUSIONS
HIF-1, APAF-1, PCDC10, FBX5, DFF40, DFFA XIAP, survivin may be regarded as the signal genes to judge the degree of hepatic ischemic-hypoxic injure, and the apoptotic liver cell injury due to ischemia in different time limits. The safe limit of human hepatic warm ischemic time appears to be generally less then 30 min.
Lu
Qi-Ping
QP
Department of General Surgery, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, Hubei Province, China.
Cao
Ting-Jia
TJ
Zhang
Zhi-Yong
ZY
Liu
Wei
W
D015536:Down-Regulation; D015870:Gene Expression; D006706:Homeostasis / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D007511:Ischemia / Q000235:genetics*; D008102:Liver Circulation; D020411:Oligonucleotide Array Sequence Analysis; D011309:Preservation, Biological / Q000009:adverse effects; D013997:Time Factors