Spectrum of bacteriocin activity of Lactobacillus plantarum BS and fingerprinting by RAPD-PCR.
The spectrum of antimicrobial activity of Lactobacillus plantarum BS against representative bacterial species was established through deferred assay and 'spot-on-lawn' assay using actively growing cells and partially purified bacteriocin extract, respectively. Only lactobacilli, pediococci, enterococci, bacilli and Listeria were inhibited from the test microorganisms. Slight bacteriocinogenic activity through 'spot-on-lawn' assay was detected against Staphylococcus aureus and Escherichia coli O157:H7. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to compare the fingerprint of L. plantarum BS with other strains of L. plantarum. Using the 16S rRNA-based primer, P32, the bacteriocinogenic isolate exhibited identical RAPD-PCR fingerprints to L. plantarum ATCC 14917. Dendrograms derived from the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) were constructed to show the similarity relationships among the investigated strains based on RAPD-PCR analysis. Bands differentiating L. plantarum BS from L. plantarum ATCC 14917 were also identified by varying the annealing temperature.
Elegado
Francisco B
FB
National Institute of Molecular Biology and Biotechnology College, University of the Philippines Los Baños, Laguna, 4031 Philippines. fbelegado@hotmail.com
Guerra
Marie Antonette Ruth V
MA
Macayan
Rommel A
RA
Mendoza
Helen A
HA
Lirazan
Marcelina B
MB
D001430:Bacteriocins / Q000494:pharmacology*; D016172:DNA Fingerprinting; D019453:Escherichia coli O157 / Q000254:growth & development*; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000254:growth & development*
D001430:Bacteriocins