Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-delta12,14-prostaglandin J2 in WISH epithelial cells.
Cyclopentenone prostaglandins, delta12-PGJ2 and 15d-PGJ2, have potent anti-tumour and anti-inflammatory activities, and have been shown to induce apoptosis in amnion-derived WISH cells. In this study, we have investigated the protective effects of serum and its constituents (growth factors and albumin) on delta12-PGJ2 and 15d-PGJ2-induced apoptosis in WISH cells. Serum (0.5% w/v) was protective against both delta12-PGJ2 and 15d-PGJ2-induced apoptosis. This was not due to the presence of serum-derived growth factors (EGF, IGF-1 and IGF-2), since they had no significant effect on 15d-PGJ2-induced cell death. In contrast, IGF-1 partially inhibited etoposide-induced apoptosis, confirming the presence of a functional IGF-1 receptor signalling system. Albumin was identified as the key survival factor in serum, since albumin and delipidated albumin exhibited the same level of protection from 15d-PGJ2-induced apoptosis as serum itself. The potential for serum albumin to regulate the bioactivity of cyclopentenone PGs may be of considerable importance in pathological conditions where roles for cyclopentenone PGs have been identified.
Berry
Elicia B E
EB
Liggins Institute, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. ebe.berry@auckland.ac.nz
Sato
Timothy A
TA
Mitchell
Murray D
MD
Stewart Gilmour
R
R
Helliwell
Rachel J A
RJ
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D009369:Neoplasms / Q000378:metabolism; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D044967:Serum; D012709:Serum Albumin / Q000494:pharmacology / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects
C477819:15-deoxyprostaglandin J2; D036341:Intercellular Signaling Peptides and Proteins; D012709:Serum Albumin; D017526:Receptor, IGF Type 1; D015230:Prostaglandin D2