title: Comparative role of proteins in transport of HCH-isomers in desert locust, Schistocerca gregaria Forskal and bovines.
abstract: The studies on binding of hexachlorocyclohexane (HCH) with carrier proteins were carried out to establish the role of proteins in the transport of insecticides in insects. Sephadex G-200 column chromatography resolved haemolymph of adult male desert locust, Schistocerca gregaria into three major protein peaks. There was significant binding of gamma-HCH with first protein peak (F1). Two classes of binding sites were observed on first protein peak for gamma-HCH. However low level of binding was observed with the third protein peak (F3) of the haemolymph. Bindings of HCH-isomers (alpha, beta and gamma) with bovine serum albumin (BSA) were not related to their water solubilities. Moderate to low affinities (1.4 -1.84 x 10(6) M(-1)) of HCH-isomers for BSA were observed. The present studies showed that more HCH binds to haemolymph lipoprotein of locust as compared to BSA. This indicates a significant role of haemolymph proteins in the transport of insecticides in insects.
authors.affiliation: Department of Entomology and Apiculture, Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, India.
authors.forename: Sapna
authors.initials: S
authors.lastname: Mahajan
authors.forename: S K
authors.initials: SK
authors.lastname: Patyal
authors.forename: S B
authors.initials: SB
authors.lastname: Ahmed
authors.forename: A
authors.initials: A
authors.lastname: Nath
mesh_terms: D000818:Animals; D002415:Cats; D006110:Grasshoppers; D001556:Hexachlorocyclohexane / Q000737:chemistry / Q000378:metabolism*; D019476:Insect Proteins / Q000502:physiology*; D007536:Isomerism
chemical_list: D019476:Insect Proteins; D001556:Hexachlorocyclohexane