Characterization of a novel selenium methyltransferase from freshwater bacteria showing strong similarities with the calicheamicin methyltransferase.
A novel group of Se-methyltransferases is presented. The genetic determinant, named mmtA, which revealed this group was isolated from selenite and selenate-resistant freshwater bacteria. E. coli expressing mmtA and grown with a Se supplement emitted dimethyl selenide (DMSe) and dimethyl diselenide (DMDSe). Phylogenetic analysis divided MmtA-like bacterial sequences into two clusters, one grouping MmtA with S- and O-methyltransferases, and one grouping UbiE C-methyltransferases. Se methylation by some of these MmtA phyletic neighbours was investigated.
Ranjard
Lionel
L
Research Group on Opportunistic Pathogens and Environment, UMR CNRS 5557 Ecologie Microbienne (Centre for Microbial Ecology), Université Claude Bernard-Lyon 1, Villeurbanne Cédex, France.
Prigent-Combaret
Claire
C
Favre-Bonté
Sabine
S
Monnez
Claire
C
Nazaret
Sylvie
S
Cournoyer
Benoit
B
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D017931:DNA Primers; D008401:Gas Chromatography-Mass Spectrometry; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014871:Water Microbiology
D017931:DNA Primers; D008780:Methyltransferases