Cloning of cDNAs encoding multiple subtypes of feline interferon-alpha from the feline epitherial cell line.
Mammalian interferon (IFN)-alpha consists of a 23-amino acid signal peptide and a 166-amino acid mature protein. Feline (Fe) IFN-alpha has an extra unique molecule consisting of a 171-amino acid mature protein with a 5-amino acid insertion. We cloned eight new subtypes of cDNA encoding FeIFN- alpha from a feline epithelial cell line. Among all the FeIFN-alpha subtypes, including six that have previously been reported, the variations were found to be far less than those of IFN-alphas of other animals.
Nagai
Ayuko
A
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan.
Taira
Osamu
O
Ishikawa
Miyuki
M
Hiramatsu
Kentaro
K
Hohdatsu
Tsutomu
T
Koyama
Hiroyuki
H
Arai
Setsuo
S
Sato
Hisaaki
H
Nakano
Katsushige
K
Maehara
Nobutoshi
N
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells; D016898:Interferon-alpha / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D016898:Interferon-alpha