Apo D in soft tissue tumors: a novel marker for dermatofibrosarcoma protuberans.
Using gene microarray expression profiling, we previously found that apolipoprotein D (Apo D) was highly expressed in dermatofibrosarcoma protuberans (DFSP). In this study, we confirm that Apo D is highly and relatively specifically expressed in DFSP using immunohistochemistry. A tissue microarray containing 421 soft tissue tumors was constructed and stained with antibodies against Apo D and CD34. Cytoplasmic immunostaining for Apo D was found in 9 of 10 typical DFSPs. In addition, 3 of 3 Bednar tumors and 2 of 3 giant cell fibroblastomas stained in conventional sections. In contrast, Apo D was immunoreactive in only a very small subset of a diverse collection of other soft tissue tumors, including Malignant Fibrous Histiocytoma (MFH), glomus tumor, neurofibroma, and malignant peripheral nerve sheath tumors. Immunostains for Apo D were negative in conventional sections of 16 fibrous histiocytomas, and an additional 12 variants of fibrous histiocytoma. Digital images of all immunohistochemical and hematoxylin and eosin tissue microarray stains are available at the accompanying website (http://microarray-pubs.stanford.edu/tma_portal/apod/). We conclude that Apo D is strongly expressed in DFSPs and neural lesions and may be useful in differentiating DFSP from fibrous histiocytoma.
West
Robert B
RB
Department of Pathology, Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA 94305, USA.
Harvell
Jeff
J
Linn
Sabine C
SC
Liu
Chih Long
CL
Prapong
Wijan
W
Hernandez-Boussard
Tina
T
Montgomery
Kelli
K
Nielsen
Torsten O
TO
Rubin
Brian P
BP
Patel
Rajiv
R
Goldblum
John R
JR
Brown
Patrick O
PO
van de Rijn
Matt
M
Lui
Chih Long
CL
D018952:Antigens, CD34 / Q000378:metabolism; D001053:Apolipoproteins / Q000096:biosynthesis*; D053399:Apolipoproteins D; D014408:Biomarkers, Tumor / Q000378:metabolism; D018223:Dermatofibrosarcoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D040081:Protein Array Analysis; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology
D018952:Antigens, CD34; D001053:Apolipoproteins; D053399:Apolipoproteins D; D014408:Biomarkers, Tumor