Hyaluronan-based biomaterial (Hyaff-11) as scaffold to support mineralization of bone marrow stromal cells.
Various techniques are widely used to repair bone defects, association of hyaluronan-based biodegradable polymers (Hyaff-11) with bone marrow stromal cells (BMSC) promises to provide successful cell scaffolds for tissue-engineered repair of bone tissue. We evaluate in vitro and in vivo the potential of Hyaff-11 to facilitate mineralization of BMSC. Rat BMSC were seeded on Hyaff-11 and their differentiation were assessed at different time points. Osteogenic differentiation was investigated in vitro analysing the expression of alkaline phosphatase and osteocalcin. Mineralization of bone defects was evaluated also in vivo implanting Hyaff-11 scaffold combined with BMSC in large segmental radius defects. In vitro, we found a decrease expression of alkaline phosphatase and an increase of osteocalcin. In vivo, our data showed that mineralization was induced and basic fibroblast growth factor contributed to this process. These results provide a demonstration to therapeutic potential of Hyaff-11 as appropriate carrier vehicle for differentiation and mineralization of BMSC and for the repair of bone defects.
Lisignoli
G
G
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Bologna, Italy.
Toneguzzi
S
S
Zini
N
N
Piacentini
A
A
Cristino
S
S
Tschon
M
M
Grassi
F
F
Fini
M
M
Giardino
R
R
Maraldi
N M
NM
Facchini
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002113:Calcification, Physiologic; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells
C086358:hyaluronic acid benzyl ester; D006820:Hyaluronic Acid