Taurine prevents streptozotocin impairment of hormone-stimulated glucose uptake in rat adipocytes.
Streptozotocin-treated rats were used as models of type 1 diabetes to study the effects of dietary taurine on insulin- and adrenergic-stimulated 2-deoxyglucose uptake by isolated adipocytes. In addition to the well-established impairment of basal and insulin-stimulated 2-deoxyglucose uptakes in adipocytes prepared from streptozotocin-diabetic rats, the alpha-(phenylephrine) and beta-(isoproterenol) adrenergic stimulations of glucose uptake were also abolished. The insulin stimulation of glucose uptake in adipocytes was selectively abolished by the phosphatidylinositol 3-kinase inhibitor wortmannin, whereas that by the adrenergic agonists, phenylephrine and isoproterenol, was inhibited by prazosin and propranolol, respectively. Dietary taurine, 4 weeks before and 4 weeks after streptozotocin administration, prevented the loss of both insulin and adrenergic agonist stimulations of 2-deoxyglucose uptake, without affecting hyperglycaemia. Because insulin and adrenergic activations of glucose transport by adipocytes are coupled to different signalling pathways, it is unlikely that these effects of taurine are related to these disparate postreceptor mechanisms.
Colivicchi
M Alessandra
MA
Department of Preclinical and Clinical Pharmacology Mario Aiazzi Mancini, University of Florence, Viale G. Pieraccini 6, Florence 50139, Italy.
Raimondi
Laura
L
Bianchi
Loria
L
Tipton
Keith F
KF
Pirisino
Renato
R
Della Corte
Laura
L
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000322:Adrenergic Agonists / Q000494:pharmacology; D018674:Adrenergic Antagonists / Q000494:pharmacology; D000818:Animals; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000097:blood / Q000494:pharmacology*
D000322:Adrenergic Agonists; D018674:Adrenergic Antagonists; D007328:Insulin; D013654:Taurine; D010656:Phenylephrine; D003847:Deoxyglucose; D011433:Propranolol; D007545:Isoproterenol; D011224:Prazosin