title: Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma.
abstract: The expression of the Wilms' tumor gene WT1 was examined by immunohistochemistry in 40 cases of pancreatic ductal adenocarcinoma. WT1 protein was expressed in 30 (75%) of the 40 pancreatic ductal adenocarcinomas, but not in the remaining 10 (25%). In normal pancreatic ductal cells, WT1 protein was undetectable. No correlations between WT1 expression and clinicopathological parameters such as age, sex, T or N stage, tumor location, and tumor differentiation were observed. Treatment with WT1 antisense oligomers significantly inhibited the growth of five human pancreatic cancer cell lines, PSN1, MiaPaCa2, ASPC1, BxPC3, and PCI6, expressing the WT1 gene. These results indicate an important role of the WT1 gene in the tumorigenesis of pancreatic ductal adenocarcinoma expressing WT1 and provide a rationale for new treatment strategies to treat pancreatic ductal adenocarcinoma by targeting the WT1 gene and its product.
authors.affiliation: Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oji
authors.forename: Shoji
authors.initials: S
authors.lastname: Nakamori
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fujikawa
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Nakatsuka
authors.forename: Asumi
authors.initials: A
authors.lastname: Yokota
authors.forename: Naoya
authors.initials: N
authors.lastname: Tatsumi
authors.forename: Sakie
authors.initials: S
authors.lastname: Abeno
authors.forename: Ai
authors.initials: A
authors.lastname: Ikeba
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takashima
authors.forename: Masanori
authors.initials: M
authors.lastname: Tsujie
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Masato
authors.initials: M
authors.lastname: Sakon
authors.forename: Riichiro
authors.initials: R
authors.lastname: Nezu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kawano
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Nishida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikegame
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawakami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tsuboi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000503:physiopathology; D000368:Aged; D000369:Aged, 80 and over; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000503:physiopathology; D002471:Cell Transformation, Neoplastic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016376:Oligonucleotides, Antisense; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000503:physiopathology; D015854:Up-Regulation; D025721:WT1 Proteins / Q000096:biosynthesis*
chemical_list: D016376:Oligonucleotides, Antisense; D025721:WT1 Proteins