title: The influence of tumor size and environment on gene expression in commonly used human tumor lines.
abstract: BACKGROUND
  The expression profiles of solid tumor models in rodents have been only minimally studied despite their extensive use to develop anticancer agents. We have applied RNA expression profiling using Affymetrix U95A GeneChips to address fundamental biological questions about human tumor lines.
  
  
  METHODS
  To determine whether gene expression changed significantly as a tumor increased in size, we analyzed samples from two human colon carcinoma lines (Colo205 and HCT-116) at three different sizes (200 mg, 500 mg and 1000 mg). To investigate whether gene expression was influenced by the strain of mouse, tumor samples isolated from C.B-17 SCID and Nu/Nu mice were also compared. Finally, the gene expression differences between tissue culture and in vivo samples were investigated by comparing profiles from lines grown in both environments.
  
  
  RESULTS
  Multidimensional scaling and analysis of variance demonstrated that the tumor lines were dramatically different from each other and that gene expression remained constant as the tumors increased in size. Statistical analysis revealed that 63 genes were differentially expressed due to the strain of mouse the tumor was grown in but the function of the encoded proteins did not link to any distinct biological pathways. Hierarchical clustering of tissue culture and xenograft samples demonstrated that for each individual tumor line, the in vivo and in vitro profiles were more similar to each other than any other profile. We identified 36 genes with a pattern of high expression in xenograft samples that encoded proteins involved in extracellular matrix, cell surface receptors and transcription factors. An additional 17 genes were identified with a pattern of high expression in tissue culture samples and encoded proteins involved in cell division, cell cycle and RNA production.
  
  
  CONCLUSIONS
  The environment a tumor line is grown in can have a significant effect on gene expression but tumor size has little or no effect for subcutaneously grown solid tumors. Furthermore, an individual tumor line has an RNA expression pattern that clearly defines it from other lines even when grown in different environments. This could be used as a quality control tool for preclinical oncology studies.
authors.affiliation: Pfizer Global Research and Development, 2800 Plymouth Rd, Ann Arbor, Michigan, 48105, USA. michael.gieseg@pfizer.com
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gieseg
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Man
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Gorski
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Madore
authors.forename: Eric P
authors.initials: EP
authors.lastname: Kaldjian
authors.forename: Wilbur R
authors.initials: WR
authors.lastname: Leopold
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D004195:Disease Models, Animal; D004777:Environment; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D013045:Species Specificity; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media