title: Newly-formed axonal branches of rat sciatic neurons sprouting in the spinal cord after peripheral axotomy.
abstract: A retrograde method of nerve tracing using a recombinant adenovirus was applied to experimental regeneration of peripheral nerves to study the sprouting position of the regenerating axon. This enabled us to see the entire length of the axons on a whole-mount neural specimen. The peroneal nerve was transsected and infected with this virus, and the tibial nerve was transsected and sutured in eight Wistar rats. Four to five weeks later, labelled axons appeared in the tibial nerve, some of which could be traced from the tibial nerve to the spinal cord without making a connection with other labelled fibres. Control experiments negated the possibilities of transneuronal immigration or contamination of the virus. When the peroneal and tibial nerves were double-labelled with fluorescent tracers four weeks after their transsection, double-labelled motor neurons appeared. Based on these findings, we conclude that regenerating branches do sprout in the spinal cord after axotomy.
authors.affiliation: Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Zenzai
authors.forename: Minoru
authors.initials: M
authors.lastname: Shibata
authors.forename: Haruo
authors.initials: H
authors.lastname: Okado
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hirano
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001369:Axons / Q000502:physiology; D019771:Axotomy; D005456:Fluorescent Dyes; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D007763:Lac Operon; D008164:Luminescent Proteins; D008297:Male; D009416:Nerve Regeneration; D010543:Peroneal Nerve / Q000502:physiology* / Q000601:surgery / Q000821:virology; D051381:Rats; D017208:Rats, Wistar; D011995:Recombination, Genetic; D013116:Spinal Cord / Q000502:physiology*; D013979:Tibial Nerve / Q000502:physiology* / Q000601:surgery / Q000821:virology
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins