title: Age-related changes of gamma-aminobutyric acid transaminase immunoreactivity in the hippocampus and dentate gyrus of the Mongolian gerbil.
abstract: We investigated the age-related changes of gamma-aminobutyric acid transaminase (GABA-T, a GABA degradation enzyme) in the hippocampus and dentate gyrus of the gerbil at postnatal month 1 (PM 1), PM 3, PM 6, PM 12, and PM 24. Age-related changes of GABA-T immunoreactivity were distinct in the hippocampal CA1 region and in the dentate gyrus. GABA-T immunoreactivity was weak at PM 1, but at PM 3, it had increased significantly, and then increased further. Between PM 6 and PM 12, strong GABA-T immunoreactivity was found in nonpyramidal cells (GABAergic) in the stratum pyramidale of the CA1 region, and at PM 6, strong GABA-T immunoreactivity was found in neurons of the dentate gyrus subgranular zone. At PM 24, CA1 pyramidal cells showed strong GABA-T immunoreactivity. Western blot analysis showed a pattern of GABA-T expression similar to that shown by immunohistochemistry at various ages. In conclusion, our results suggest that the age-related changes of GABA-T provide important information about the aged brain with GABA dysfunction.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Duk Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count; D018891:Dentate Gyrus / Q000201:enzymology*; D005849:Gerbillinae; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology*; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D000612:4-Aminobutyrate Transaminase; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1