title: Prognostic significance of p53 and FHIT in advanced oropharyngeal carcinoma.
abstract: OBJECTIVE
  To determine the prognostic significance of p53 and fragile histidine triad (FHIT) expression in advanced oropharyngeal squamous cell carcinoma.
  
  
  METHODS
  A retrospective collection of clinical data was correlated with the protein expression.
  
  
  METHODS
  The expression of p53 and FHIT in specimens from patients with previously untreated advanced squamous cell carcinoma of the oropharynx was determined by immunohistochemistry. The expression of p53 and FHIT was statistically correlated with survival outcome. The primary endpoints were overall survival and disease-free survival.
  
  
  RESULTS
  Thirty-four patients were analyzed in this study. Overexpression of p53 was observed in 41.2% (14/34) of tumors and was associated with a trend toward an improved overall survival using univariate (P =.1088, risk ratio [RR] = 0.503) and multivariate (P =.1533, RR = 0.470) analyses. Marked reduction or complete absence of FHIT expression was observed in 57.6% (19/33) of tumors. Patients with tumors showing no reduction in FHIT expression had a lower overall survival using univariate (P =.04, RR = 2.27) and multivariate (P =.013, RR = 4.41) analyses.
  
  
  CONCLUSIONS
  Overexpression of p53 predicted a trend toward an improved prognosis, whereas no reduction in FHIT expression predicted a significantly poorer outcome in patients with advanced oropharyngeal cancer.
authors.affiliation: Department of Otolaryngology, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Jose E
authors.initials: JE
authors.lastname: Otero-Garcia
authors.forename: Emad
authors.initials: E
authors.lastname: Youssef
authors.forename: Ileana I
authors.initials: II
authors.lastname: Enamorado
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: George H
authors.initials: GH
authors.lastname: Yoo
authors.forename: Kambiz
authors.initials: K
authors.lastname: Merati
authors.forename: Danny
authors.initials: D
authors.lastname: Kewson
authors.forename: Fulvio
authors.initials: F
authors.lastname: Lonardo
authors.forename: John R
authors.initials: JR
authors.lastname: Jacobs
authors.forename: Harold
authors.initials: H
authors.lastname: Kim
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009959:Oropharyngeal Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D012189:Retrospective Studies; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; D000099170:Bis(5'-adenosyl)triphosphatase