Yeast display of antibody fragments: a discovery and characterization platform.
Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a non-immune scFv and immune Fab libraries. A major strength of yeast display as a novel antibody discovery platform is the ability to characterize the binding properties, i.e., the affinity and epitope binding characteristics, of a clone without the need for subcloning, expression and purification of the scFv. This review focuses on novel attributes of yeast display for antibody engineering endeavors.
Feldhaus
Michael J
MJ
Pacific Northwest National Laboratory, MSIN:K4-12, 902 Battelle Boulevard, P.O. Box 999, Richland, WA 99352, USA. feldhaus.michael@pnl.gov
Siegel
Robert W
RW
D000818:Animals; D019020:Directed Molecular Evolution / Q000379:methods*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics* / Q000276:immunology; D019151:Peptide Library; D011233:Precipitin Tests; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D007128:Immunoglobulin Fragments; D019151:Peptide Library