Establishment of a new scirrhous gastric carcinoma cell line with microsatellite instability.
We report on the establishment of a new scirrhous gastric cancer cell line with microsatellite instability (MSI), designated OCUM-7. This cell line was derived from a primary scirrhous gastric carcinoma. The cells were floating and round shape in culture. Histologic findings of xenografted tumor obtained from OCUM-7 cells showed a poorly differentiated adenocarcinoma with medullary growth. DNA histograms of OCUM-7 cells showed an aneuploid pattern. MSI status of OCUM-7 was analyzed using 8 microsatellite markers. Five of 8 microsatellite loci showed a novel band shift, which demonstrated that OCUM-7 cells were MSI-high. MSI-positive cell lines established from a primary scirrhous gastric carcinoma have not been reported, while several reports of the establishment of a scirrhous gastric cancer cell line are available. OCUM-7 might be beneficial for analyzing the mechanisms of MSI in scirrhous gastric carcinoma.
Koyama
Tsuyoshi
T
Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
Yashiro
Masakazu
M
Matsuoka
Tasuku
T
Sunami
Takeshi
T
Ohira
Masaichi
M
Hirakawa
Kosei
K
D002293:Adenocarcinoma, Scirrhous / Q000235:genetics* / Q000473:pathology; D000368:Aged; D000951:Antigens, Neoplasm; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002871:Chromosome Banding; D043243:DNA Sequence, Unstable; D016368:Frameshift Mutation; D006801:Humans; D007621:Karyotyping; D008297:Male; D018895:Microsatellite Repeats; D008858:Microscopy, Phase-Contrast; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D000951:Antigens, Neoplasm