Anaerobic growth of the haloalkaliphilic denitrifying sulfur-oxidizing bacterium Thialkalivibrio thiocyanodenitrificans sp. nov. with thiocyanate.
Two strains of obligate chemolithoautotrophic sulfur-oxidizing bacteria were isolated from soda-lake sediments by enrichment culture with thiocyanate and nitrate at pH 9.9. The isolates were capable of growth with thiocyanate or thiosulfate as electron donor, either aerobically or anaerobically, and with nitrate or nitrite as electron acceptor. Cyanate was identified as an intermediate of thiocyanate oxidation, while sulfate, ammonia and dinitrogen gas were the final products. The anaerobic growth on thiocyanate plus nitrate was much slower (mu(max)=0.006 h(-1)) than on thiosulfate plus nitrate (mu(max)=0.02 h(-1)), while growth yields were similar (4.8 and 5.1 g protein mol(-1), respectively). On the basis of their phenotypic and genetic properties, strains ARhD 1(T) and ARhD 2 are described as a novel species of the genus Thialkalivibrio, with the highest similarity to Thialkalivibrio denitrificans. The name Thialkalivibrio thiocyanodenitrificans sp. nov. is proposed for this novel species.
Sorokin
Dimitry Yu
DY
Department of Environmental Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.|Institute of Microbiology RAS, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.
Tourova
Tat'yana P
TP
Institute of Microbiology RAS, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.
Antipov
Alexey N
AN
A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Muyzer
G
G
Department of Environmental Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Kuenen
J Gijs
JG
Department of Environmental Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
D000693:Anaerobiosis; D003470:Culture Media; D005618:Fresh Water / Q000382:microbiology; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013861:Thiocyanates / Q000378:metabolism*
D003470:Culture Media; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013861:Thiocyanates; D012965:Sodium Chloride; D013455:Sulfur; C031760:thiocyanate