Vasopressin phosphorylates HSP27 in aortic smooth muscle cells.
Administration of arginine vasopressin (AVP) time-dependently induced the phosphorylation of heat shock protein 27 (HSP27) at Ser-15 and Ser-85 in smooth muscle of aorta in vivo. The AVP-induced phosphorylation of HSP27 at Ser-15 and Ser-85 was inhibited by a V1a receptor antagonist but not by a V2 receptor antagonist. In cultured aortic smooth muscle A10 cells, AVP markedly stimulated the phosphorylation of HSP27 at Ser-15 and Ser-85. The AVP-induced phosphorylation of HSP27 was attenuated by SB203580 and PD169316, inhibitors of p38 mitogen-activated protein (MAP) kinase, but not by PD98059, a MEK inhibitor. These results strongly suggest that AVP phosphorylates HSP27 via p38 MAP kinase in aortic smooth muscle cells.
Akamatsu
Shigeru
S
Department of Critical Care Medicine, Gifu University School of Medicine, Gifu, Japan.
Nakajima
Keiichi
K
Ishisaki
Akira
A
Matsuno
Hiroyuki
H
Tanabe
Kumiko
K
Takei
Mariko
M
Takenaka
Motoki
M
Hirade
Kouseki
K
Yoshimi
Naoki
N
Suga
Hidetaka
H
Oiso
Yutaka
Y
Kato
Kanefusa
K
Kozawa
Osamu
O
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001127:Arginine Vasopressin / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D006360:Heat-Shock Proteins; D007093:Imidazoles; D011725:Pyridines; D001127:Arginine Vasopressin; C408604:2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole; C093642:SB 203580