Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins.
We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKC from a perinuclear site to the nucleus, extending findings in PC12 and NG108-15 cells and in myocytes. We have immunoprecipitated the PKC from nuclei isolated from phorbol ester-treated fibroblasts and identified six proteins which associate with nuclear PKC. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, beta-actin and annexin II by MALDI-TOF-MS. We have confirmed that these proteins associate with PKC by gel overlay and/or dot blotting assays. The role of these PKC-associating proteins in the nucleus and their interaction with PKC are considered.
Xu
Tian-Rui
TR
Department of Biology, University of York, York YO10 5DD, UK.
Rumsby
Martin G
MG
D000199:Actins / Q000378:metabolism; D021581:Active Transport, Cell Nucleus; D000818:Animals; D017306:Annexin A2 / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D007074:Immunoglobulin G / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D032342:Muscle Cells / Q000378:metabolism; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000378:metabolism; D010703:Phorbol Esters / Q000378:metabolism*; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000378:metabolism*; D051744:Protein Kinase C-epsilon; D021381:Protein Transport; D016601:RNA-Binding Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014168:Transferrin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D017306:Annexin A2; D007074:Immunoglobulin G; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D010703:Phorbol Esters; D016601:RNA-Binding Proteins; D014168:Transferrin; D014746:Vimentin; C068873:matrin 3, rat; D049452:Green Fluorescent Proteins; C497256:Prkce protein, mouse; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon