title: Distribution of short paired duplications in mammalian genomes.
abstract: Mammalian genomes are densely populated with long duplicated sequences. In this paper, we demonstrate the existence of doublets, short duplications between 25 and 100 bp, distinct from previously described repeats. Each doublet is a pair of exact matches, separated by some distance. The distribution of these intermatch distances is strikingly nonrandom. An unexpectedly high number of doublets have matches either within 100 bp (adjacent) or at distances tightly concentrated approximately 1,000 bp apart (nearby). We focus our study on these proximate doublets. First, they tend to have both matches on the same strand. By comparing nearby doublets shared in human and chimpanzee, we can also see that these doublets seem to arise by an insertion event that produces a copy without markedly affecting the surrounding sequence. Most doublets in humans are shared with chimpanzee, but many new pairs arose after the divergence of the species. Doublets found in human but not chimpanzee are most often composed of almost tandem matches, whereas older doublets (found in both species) are more likely to have matches spaced by approximately 1 kb, indicating that the nearly tandem doublets may be more dynamic. The spacing of doublets is highly conserved. So far, we have found clearly recognizable doublets in the following genomes: Homo sapiens, Mus musculus, Arabidopsis thaliana, and Caenorhabditis elegans, indicating that the mechanism generating these doublets is widespread. A mechanism that generates short local duplications while conserving polarity could have a profound impact on the evolution of regulatory and protein-coding sequences.
authors.affiliation: Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Thomas
authors.forename: Nathan
authors.initials: N
authors.lastname: Srebro
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sebat
authors.forename: Nicholas
authors.initials: N
authors.lastname: Navin
authors.forename: John
authors.initials: J
authors.lastname: Healy
authors.forename: Bud
authors.initials: B
authors.lastname: Mishra
authors.forename: Michael
authors.initials: M
authors.lastname: Wigler
mesh_terms: D000818:Animals; D017360:Arabidopsis; D001483:Base Sequence; D017173:Caenorhabditis elegans; D004251:DNA Transposable Elements / Q000235:genetics; D019143:Evolution, Molecular; D020440:Gene Duplication; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D002679:Pan troglodytes
chemical_list: D004251:DNA Transposable Elements