title: HPV DNA test and Pap smear in detection of residual and recurrent disease following loop electrosurgical excision procedure of high-grade cervical intraepithelial neoplasia.
abstract: OBJECTIVE
  We compared the performance of cervical cytology and HPV DNA test in detection of residual or recurrent disease following the treatment of cervical intraepithelial neoplasia (CIN) 2/3 with loop electrosurgical excision procedure (LEEP).
  
  
  METHODS
  A series of 107 women subjected to LEEP due to histologically confirmed CIN 2/3 between March 2001 and December 2002 were followed-up biannually until January 2004. Follow-up visits consisted of interview and gynecological examination including cervical cytology, hybrid capture II (HCII), and colposcopy. Patients presenting with abnormal colposcopy or high-grade squamous intraepithelial lesion (HSIL) smear were subjected to new excision procedure, and presence of histologically confirmed CIN 2/3 or higher was considered as residual or recurrent disease. Performance indicators were calculated for cytology and HCII assay in detecting residual or recurrent disease.
  
  
  RESULTS
  Eleven (10.2%) women showed residual or recurrent disease during the follow-up. Considering HCII and Pap smear as stand-alone tests, both techniques showed similar sensitivity, detecting 100% of CIN 2/3 at the first follow-up visit. At the second follow-up visit, Pap smear showed better specificity and positive predictive value (PPV) than HCII, and both tests had fairly the same high negative predictive value (NPV) and sensitivity. The combined positive HCII and abnormal cytology had the same sensitivity as each of the tests alone, but specificity and PPV were significantly higher than those of single tests. When only one of the tests was positive, the sensitivity and the NPV of the combination remained the same, but its specificity and PPV were lower than that of the combined two positive tests and that of the individual test, at both follow-up visits.
  
  
  CONCLUSIONS
  Both tests performed well in detecting residual or recurrent disease after LEEP and combination of the tests did not increase sensitivity of the single tests.
authors.affiliation: Obstetrics and Gynecology Department, Universidade Estadual de Campinas, UNICAMP, Campinas, São Paulo 13083-370, Brazil.
authors.forename: Luís Otávio
authors.initials: LO
authors.lastname: Sarian
authors.forename: Sophie F M
authors.initials: SF
authors.lastname: Derchain
authors.forename: Liliana A A
authors.initials: LA
authors.lastname: Andrade
authors.forename: Júlia
authors.initials: J
authors.lastname: Tambascia
authors.forename: Sirlei Siani
authors.initials: SS
authors.lastname: Morais
authors.forename: Kari J
authors.initials: KJ
authors.lastname: Syrjänen
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis*; D004598:Electrosurgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D018365:Neoplasm, Residual; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery* / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery* / Q000821:virology
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic