Production of bioactive cellulose films reconstituted from ionic liquids.
A new method for introducing enzymes into cellulosic matrixes which can be formed into membranes, films, or beads has been developed using a cellulose-in-ionic-liquid dissolution and regeneration process. Initial results on the formation of thin cellulose films incorporating dispersed laccase indicate that active enzyme-encapsulated films can be prepared using this methodology and that precoating the enzyme with a second, hydrophobic ionic liquid prior to dispersion in the cellulose/ionic liquid solution can provide an increase in enzyme activity relative to that of untreated films, presumably by providing a stabilizing microenvironment for the enzyme.
Turner
Megan B
MB
Center for Green Manufacturing and Department of Chemistry, The University of Alabama, Tuscaloosa, Alabama 35487, USA.
Spear
Scott K
SK
Holbrey
John D
JD
Rogers
Robin D
RD
D002384:Catalysis; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D007477:Ions / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry; D008567:Membranes, Artificial; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012997:Solvents / Q000737:chemistry*; D013499:Surface Properties
D007477:Ions; D008567:Membranes, Artificial; D012997:Solvents; D002482:Cellulose; D042845:Laccase