An optimized CZE method for analysis of mono- and oligomeric aldose mixtures.
An optimized capillary electrophoresis (CZE) method to analyze complex mixtures of aldoses was developed. The approach allows simultaneous quantitative analysis of all four isomeric aldopentoses, eight aldohexoses, as well as xylo- and cellooligosaccharides up to the tetraoses. UV tagging with 4-aminobenzoic acid ethyl ester (ABEE) in combination with reductive amination was used as pre-column derivatization. With optimum baseline separation and short run times, the method is very robust, and especially suited to follow reaction and isomerization kinetics of monosaccharides.
Sjöberg
John
J
Christian-Doppler-Laboratory, Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria.
Adorjan
Immanuel
I
Rosenau
Thomas
T
Kosma
Paul
P
D000447:Aldehydes / Q000737:chemistry*; D002236:Carbohydrate Conformation; D019075:Electrophoresis, Capillary / Q000379:methods; D006601:Hexoses / Q000737:chemistry; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D010429:Pentoses / Q000737:chemistry; D013997:Time Factors
D000447:Aldehydes; D006601:Hexoses; D009844:Oligosaccharides; D010429:Pentoses