An enzymatic route to produce pyruvate from lactate.
A bacterial strain of Acinetobacter sp., which was capable of enzymatic production of pyruvate from lactate, was cultured in a 5-l reactor with a basal salt medium. After 14 h of fed-batch fermentation, 9.56 g l(-1) cell concentration in the broth was obtained with 20 g l(-1) (178 mM) sodium lactate and 4 g l(-1) NH(4)Cl in the medium; and the biotransformation ability was 2.51 units ml(-1). The cells were harvested from one reactor and then used for pyruvate production from lactate in the same reactor. l-lactate at a concentration about 527 mM was almost stoichiometrically converted to pyruvate in 28 h. After a total 42 h of cell culture and biotransformation, the transformative yield was about 0.72 g g(-1) pyruvate from lactate and the rate of pyruvate production was calculated as 1.33 g l(-1) h(-1) during the process. The results suggested this simple enzymatic production of pyruvate from lactate should be a promising process and may bring a yield higher than that by microbial fermentation. By this process, the recovery of pyruvate from such a simple reaction liquid is relatively easy and inexpensive to perform.
Ma
C Q
CQ
State Key Laboratory of Microbial Technology, Shandong University, 250100 Jinan, People's Republic of China.
Xu
P
P
Qiu
J H
JH
Zhang
Z J
ZJ
Wang
K W
KW
Wang
M
M
Zhang
Y N
YN
D000150:Acinetobacter / Q000201:enzymology* / Q000254:growth & development; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D049109:Cell Proliferation; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism*; D013997:Time Factors; D014867:Water / Q000378:metabolism
D007773:Lactates; D011773:Pyruvates; D014867:Water; D002244:Carbon; D009584:Nitrogen