Development of PCR assay to identify Pseudomonas fluorescens and its biotype.
The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production.
Scarpellini
Mauro
M
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sez Microbiologia Agraria Alimentare Ecologica, Via Celoria 2, 20133 Milano, Italy. mauro.scarpellini@unimi.it
Franzetti
Laura
L
Galli
Antoniëtta
A
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005630:Fructans / Q000096:biosynthesis; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011551:Pseudomonas fluorescens / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D005630:Fructans; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C072599:levan; C052712:endodeoxyribonuclease VspI; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases