title: Elevated urinary 8-isoprostaglandin F(2alpha) in females with Graves' hyperthyroidism.
abstract: In this study, we investigate how oxidative stress alters the urinary F2-isoprostanes' level and we examine the correlation between 8-isoPGF(2alpha) and thyroid hormones in female patients with Graves' hyperthyroidism. We quantitatively determined the concentrations of urinary F2-isoprostanes using gas chromatography-mass spectrometry in the selected ion-monitoring mode. We recruited individuals in the following three groups of subjects for this study: (1) female hyperthyroidism patients (n = 14, 21-71 years), (2) female hypothyroidism patients (n = 16, 16-66 years), and (3) female age-matched normal controls (n = 10, 20-61 years). The average concentration of 8-iso prostaglandin F(2alpha) (8-isoPGF(2alpha)) in hyperthyroidism patients was significantly higher than that in the healthy controls (P < 0.05). The concentration of 8-isoPGF(2alpha) in hypothyroidism patients was similar to that in normal controls. Although the level of 8-isoPGF(2alpha) in two of the patients was slightly elevated, the P value was not significant (NS). Our data suggest that the increased level of urinary 8-isoPGF(2alpha) may reflect possible oxidation injuries in hyperthyroidism patients due to the high level of thyroid hormones. Therefore, the elevated 8-isoPGF(2alpha) in patients could be used as an important biomarker for hyperthyroidism disease.
authors.affiliation: Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, South Korea.
authors.forename: Kyung Mee
authors.initials: KM
authors.lastname: Kim
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Paeng
authors.forename: Jong Ryeal
authors.initials: JR
authors.lastname: Hahm
authors.forename: Deok Ryong
authors.initials: DR
authors.lastname: Kim
authors.forename: Bong Chul
authors.initials: BC
authors.lastname: Chung
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006111:Graves Disease / Q000652:urine*; D006801:Humans; D007037:Hypothyroidism / Q000652:urine; D008875:Middle Aged; D011237:Predictive Value of Tests; D013963:Thyroid Hormones / Q000378:metabolism
chemical_list: D015415:Biomarkers; D013963:Thyroid Hormones; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost