Effects of TGF beta1 autocrine blockage on osteosarcoma cells.
OBJECTIVE
To evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.


RESULTS
The proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.


CONCLUSIONS
Blockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.
Liu
Yong
Y
Department of Orthopaedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022. lw7812@21cn.com
Zheng
Qi-Xin
QX
Du
Jing-Yuan
JY
Yang
Shu-Hua
SH
Shao
Zeng-Wu
ZW
Xiao
Bao-Jun
BJ
D000970:Antineoplastic Agents / Q000494:pharmacology; D016375:Antisense Elements (Genetics) / Q000096:biosynthesis / Q000235:genetics; D019898:Autocrine Communication / Q000187:drug effects*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*; D053773:Transforming Growth Factor beta1
D000970:Antineoplastic Agents; D016375:Antisense Elements (Genetics); D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D004317:Doxorubicin