Surfactant-enhanced transdermal delivery by electroporation.
The objective of the experiment was to study the influence of sodium dodecyl sulfate (SDS) on transdermal transport of diffusants by electroporation. The resistance of porcine epidermis in contact with SDS solution (0.2% w/v) dropped by 40% within 24 h. SDS improved the efficiency of transdermal delivery of glucose, dextrans of molecular weight (MW) 4 kDa (FD4K) and 10 kDa (FD10K) by electroporation. However, the transport of dextran MW 35 kDa (FD35K) was not influenced significantly. Pretreatment of epidermis with SDS solution reduced its electroporation threshold from 80 to 60 V. It appears that presence of SDS during electroporation helps in achieving the desired transport with less electrical exposure dose. SDS enhanced the transdermal delivery of molecules by electroporation most likely by facilitating the barrier disruption during pulse application and also by prolonging the lifetime of electropores created by the pulse.
Murthy
S Narasimha
SN
Molecular and Cellular Biophysics Department, Roswell Park Cancer Institute, Elm and Carlton St., Buffalo, NY 14263, USA. Snarasimha.murthy@roswellpark.org
Sen
Arindam
A
Hui
Sek Wen
SW
D000279:Administration, Cutaneous; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003911:Dextrans / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D017097:Electric Impedance; D004560:Electricity; D018274:Electroporation / Q000379:methods*; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*; D013552:Swine
D003911:Dextrans; D013501:Surface-Active Agents; C015219:fluorescein isothiocyanate dextran; D012967:Sodium Dodecyl Sulfate; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose