Automated ribotyping, a rapid typing method for analysis of Erysipelothrix spp. strains.
Automated ribotyping classified 70 Erysipelothrix species strains, previously classified into 14 RAPD patterns and into 63 PFGE patterns, into 27 ribogroups. Twenty-three strains of the 70 analyzed and classified into 13 ribogroups were previously classified into six ribotypes by the traditional ribotyping method. Moreover, automated ribotyping differentiated seven strains that were not differentiated by PFGE. Therefore, automated ribotyping was more sensitive than RAPD and traditional ribotyping, and it might be a useful method for a rapid screening in epidemiological study of strains of this genus, and more accurate results can be obtained when this method is used together with PFGE.
Okatani
Tomomitsu Alexandre
TA
Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Ishikawa
Motoko
M
Yoshida
Shin-Ichiro
S
Sekiguchi
Michiko
M
Tanno
Kenji
K
Ogawa
Masuo
M
Horikita
Tetsuya
T
Horisaka
Tomoko
T
Taniguchi
Takahide
T
Kato
Yukio
Y
Hayashidani
Hideki
H
D001331:Automation / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004888:Erysipelothrix / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D021521:Ribotyping / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S