title: Comparing the clinical outcomes of intrauterine insemination by two different density gradient preparation methods.
abstract: BACKGROUND
  Sperm preparation has play an integral part in the success of in-vitro fertilization. The aim of this study was to compare 2 different density gradient preparations for sperm separation in respect to sperm recovery, motility, motion parameters and clinical outcome after intrauterine insemination.
  
  
  METHODS
  One-hundred and 21 women who received intrauterine insemination due to ovulation dysfunction were randomly allocated into 2 groups, using either the Percoll (Amersham, Pharmacia Biotech AB, Sweden) or the PureSperm (Nidacon, Göteborg, Sweden) density gradient method for sperm preparation. The characteristics of sperm before and after separation and the clinical outcome of intrauterine insemination were compared between the 2 groups.
  
  
  RESULTS
  PureSperm and Percoll demonstrated comparable ability to recover the sperms with progressive motility. There was no difference in motion parameters and the number of sperm recovered with progressive motility between the Percoll and the PureSperm density gradient preparations. The clinical pregnancy rate was also comparable between the 2 groups, 12.5% (7/56) in the PureSperm group compared to 13.8% (9/65) in the Percoll group, (p > 0.05).
  
  
  CONCLUSIONS
  Despite using different density composition and volume, PureSperm demonstrated clinical effect comparable to that of Percoll in preparing sperm for intrauterine insemination.
authors.affiliation: Department of Obstetrics and Gynecology, Chi Mei Foundation Medical Center, Tainan, Taiwan, ROC. yung0613@ms2.hinet.net
authors.forename: Yung-Chieh
authors.initials: YC
authors.lastname: Tsai
authors.forename: Mike Yan-Sheng
authors.initials: MY
authors.lastname: Lin
authors.forename: Sheng-Hsieg
authors.initials: SH
authors.lastname: Chen
authors.forename: Chieh-Yi
authors.initials: CY
authors.lastname: Kang
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Tsai
authors.forename: Liang-Yin
authors.initials: LY
authors.lastname: Lin
authors.forename: Loo-Tao
authors.initials: LT
authors.lastname: Chuan
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Huang
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D006080:Ovarian Follicle / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate; D013074:Sperm Banks / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology