Founder myoblasts and fibre number during adult myogenesis in Drosophila.
We have examined the mechanisms underlying the setting of myotubes and choice of myotube number in adult Drosophila. We find that the pattern of adult myotubes is prefigured by a pattern of duf-lacZ-expressing myoblasts at appropriate locations. Selective expression of duf-lacZ in single myoblasts emerges from generalized, low-level expression in all adult myoblasts during the third larval instar. The number of founders, thus chosen, corresponds to the number of fibres in a muscle. In contrast to the embryo, the selection of individual adult founder cells during myogenesis does not depend on Notch-mediated lateral inhibition. Our results suggest a general mechanism by which multi-fibre muscles can be patterned.
Dutta
Devkanya
D
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India.
Anant
Sumita
S
Ruiz-Gomez
Mar
M
Bate
Michael
M
VijayRaghavan
K
K
D000818:Animals; D019521:Body Patterning; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D007814:Larva / Q000166:cytology / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000502:physiology; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C497759:N protein, Drosophila; C090146:Rac1 protein, Drosophila; D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; C413082:kirre protein, Drosophila; D020744:rac GTP-Binding Proteins