title: Simultaneous determination of four bufadienolides in human liver by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method for the simultaneous quantitation of four bufadienolides-cinobufotalin, bufalin, cinobufagin and resibufogenin-in human liver was investigated. The procedure involved solid phase extraction of human liver with an Oasis HLB cartridge coupled with reversed-phase HPLC and photodiode array detection. Recoveries obtained from spiked liver for the bufadienolides were better than 70%. The linearity was studied up to 1.2 mg/kg and the detection limits of the method were 0.4 ng for cinobufotalin and bufalin and 0.5 ng for cinobufagin and resibufogenin. The developed method was successfully applied to a lethal poisoning case.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Wen
authors.forename: Jizong
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D000328:Adult; D002018:Bufanolides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008099:Liver / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002018:Bufanolides