Analysis of p53 mutations in cells taken from paraffin-embedded tissue sections of ductal carcinoma in situ and atypical ductal hyperplasia of the breast.
Mutations in the p53 tumor suppressor gene are frequent in breast tumors but the implication of p53 mutations in breast cancer development remains poorly understood. In this study, we applied laser capture microdissection (LCM) microscope to histologically review and sample cells from paraffin-embedded breast tissue sections obtained from six cases of ductal carcinoma in situ (DCIS) and ten cases of atypical ductal hyperplasia (ADH). p53 mutations were detected, using single stranded conformational polymorphism (SSCP) and sequencing, in cell samples of three cases with DCIS and five cases with ADH. p53 mutations are therefore present in DCIS and ADH of the breast, considered as pre-malignant precursors to breast cancer, and some of them may represent early events in breast cancer development.
Keohavong
Phouthone
P
Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, 3343 Forbes Avenue, Pittsburgh, PA 15260, USA. pho1@pitt.edu
Gao
Wei-Min
WM
Mady
Hussam H
HH
Kanbour-Shakir
Amal
A
Melhem
Mona F
MF
D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D004252:DNA Mutational Analysis; D005260:Female; D016158:Genes, p53; D006801:Humans; D006965:Hyperplasia; D042282:Microdissection; D016612:Paraffin Embedding; D018807:Polymorphism, Single-Stranded Conformational; D011230:Precancerous Conditions