title: [Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome].
abstract: OBJECTIVE
  To prepare podophyllotoxin-dipalmitoylphosphatidylcholine (PPT-DPPC) proliposomes (PPT-DPPC-PL) for improvement of the stability of PPT-DPPC liposome.
  
  
  METHODS
  Freeze-drying method was used to prepare PPT-DPPC-PL, and the particle morphology, size range, encapsulation efficiency and stability of PPT-DPPC liposome were investigated.
  
  
  RESULTS
  After hydration of PPT-DPPC-PL, PPT-DPPC liposome appeared multivesicular under electron microscope and the particles were distributed homogeneously with an average particle size of 1.45+/-0.38 microm. The encapsulation efficiency of PPT was 72.3%, and after storage at 4 to 40 degrees Celsius; for 1 to 6 months, the proliposome remained stable.
  
  
  CONCLUSIONS
  The prepared PPT-DPPC-PL particles by freeze-drying method are evenly distributed. The preparation method is relatively simple with higher embedding ratio and better stability.
authors.affiliation: Department of Dermatology, Department of Pharmacy, First Military Medical University, Guangzhou 510515, China. achang3333@sohu.com
authors.forename: Zhong-fang
authors.initials: ZF
authors.lastname: Lin
authors.forename: Kang
authors.initials: K
authors.lastname: Zeng
authors.forename: Zai-gao
authors.initials: ZG
authors.lastname: Zhou
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
authors.forename: Fang-ming
authors.initials: FM
authors.lastname: Xie
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Zhu
authors.forename: San-quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000008:administration & dosage*; D004355:Drug Stability; D005612:Freeze Drying; D008081:Liposomes; D010316:Particle Size; D011034:Podophyllotoxin / Q000008:administration & dosage*; D013696:Temperature
chemical_list: D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D011034:Podophyllotoxin