Preparation and application of cysteine-capped ZnS nanoparticles as fluorescence probe in the determination of nucleic acids.
Cysteine-capped ZnS nanometer-sized fluorescent particles were produced by a colloidal aqueous synthesis. The functionalized nanoparticles are water-soluble and suitable for biological application. A synchronous fluorescence method has been developed for the rapid determination of DNA with functionalized nano-ZnS as a fluorescence probe, based on the synchronous fluorescence enhancement of cysteine-capped nano-ZnS in the presence of DNA. When Deltalambda =190 nm, maximum synchronous fluorescence is produced at 267 nm at pH 5.12. Under optimum conditions, the synchronous fluorescence intensity is proportional to the concentration of nucleic acids in the range 0.1-1.2 microg ml(-1) for calf thymus DNA, 0.1-0.6 microg ml(-1) for fish sperm DNA. The corresponding detection limit is 32.9 ng ml(-1) for calf thymus DNA and 24.6 ng ml(-1) for fish sperm DNA. This method is simple, inexpensive, rapid and sensitive. The recovery and relative standard deviation are satisfactory.
Li
Yongxin
Y
College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China. liyongxin@hotmail.com
Chen
Jinlong
J
Zhu
Changqin
C
Wang
Lun
L
Zhao
Danhua
D
Zhuo
Shujuan
S
Wu
Yuqin
Y
D000818:Animals; D002417:Cattle; D003102:Colloids; D003545:Cysteine / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D005399:Fishes; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000302:isolation & purification*; D008297:Male; D008854:Microscopy, Electron; D049329:Nanostructures; D036103:Nanotechnology; D009696:Nucleic Acids / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013094:Spermatozoa / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D013950:Thymus Gland / Q000737:chemistry; D014867:Water; D017967:Zinc Compounds / Q000737:chemistry*
D003102:Colloids; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D013440:Sulfides; D017967:Zinc Compounds; D014867:Water; D004247:DNA; D003545:Cysteine; C031238:zinc sulfide