Isopentenyl pyrophosphate-reactive Vgamma9Vdelta 2 T helper 1-like cells are the major gammadelta T cell subset recovered from lesions of patients with genital herpes.
Herpes simplex virus (HSV)-specific T cells are essential to control and resolve genital herpes (GH). To investigate the potential involvement of gamma delta T cells in GH, T cells were recovered and expanded, by mitogenic stimulation, to T cell lines from the genital lesions of 17 patients with GH and 5 control subjects who had other diseases. Relatively high numbers of gamma delta T cells--predominantly, V gamma 9V delta 2 T cells--were detected only in the T cell lines of the patients with GH. Intralesional V gamma 9V delta 2 T cell clones did not recognize HSV-infected cells, but they showed reactivity to isopentenyl pyrophosphate and Daudi cells. The T cell clones secreted interferon- gamma, tumor necrosis factor- alpha, interleukin (IL)-8, macrophage inflammatory protein-1 alpha, and RANTES (regulated on activation, normally T cell expressed or secreted), but they secreted no or limited IL-4. The results of the present study suggest the infiltration and putative involvement of isopentenyl pyrophosphate-reactive V gamma 9V delta 2 T helper 1-like cells in individuals with GH.
Verjans
Georges M G M
GM
Institute of Virology, Erasmus Medical Center, Rotterdam, The Netherlands. g.verjans@erasmusmc.nl
Roest
R Wim
RW
van der Kooi
Alex
A
van Dijk
Grietje
G
van der Meijden
Wim I
WI
Osterhaus
Albert 'D M E
A'
D000293:Adolescent; D000328:Adult; D001003:Anal Canal / Q000276:immunology / Q000821:virology; D001706:Biopsy; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005260:Female; D045782:Hemiterpenes / Q000276:immunology*; D006558:Herpes Genitalis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009943:Organophosphorus Compounds / Q000276:immunology*; D010413:Penis / Q000276:immunology / Q000821:virology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D013048:Specimen Handling; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D014844:Vulva / Q000276:immunology / Q000821:virology
D016207:Cytokines; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D016692:Receptors, Antigen, T-Cell, gamma-delta; C004809:isopentenyl pyrophosphate