Expression of TRAIL, DR4, and DR5 in kidney and serum from patients receiving renal transplantation.
Renal transplantation is the best treatment of some end-stage renal diseases. Unfortunately, not every transplant is successful due to the rejection or dysfunction of the transplanted kidney. Many cytokines participate in rejection by inducing inflammation or apoptosis. In this study, the expressions of TRAIL, DR4, and DR5 in rejected renal tissue and of serum soluble TRAIL (sTRAIL) in patients with kidney rejection were investigated by immunohistochemical staining and sandwich enzyme-linked immunosorbent assay, respectively. The results showed that the expression of TRAIL, DR4 and DR5, and serum sTRAIL levels were markedly upregulated among renal transplant patients. Since both membrane and soluble forms of TRAIL can induce apoptosis of DR4/DR5-expressing cells via recruiting FADD and caspase 8, elevated TRAIL and its receptors may participate in renal graft rejection.
Song
C J
CJ
Department of Immunology, Fourth Military Medical University, Xi'an, P. R. People's Republic of China.
Liu
X S
XS
Zhu
Y
Y
Chen
L H
LH
Jia
W
W
Li
Y N
YN
Cao
Y X
YX
Xie
X
X
Zhuang
R
R
Zhu
C S
CS
Jin
B Q
BQ
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D015804:HLA-DR4 Antigen / Q000032:analysis* / Q000097:blood / Q000235:genetics; D015805:HLA-DR5 Antigen / Q000032:analysis* / Q000097:blood / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000097:blood / Q000235:genetics; D012016:Reference Values; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; D015804:HLA-DR4 Antigen; D015805:HLA-DR5 Antigen; D008562:Membrane Glycoproteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha