Validation of a PCR-based method for detection of food-borne thermotolerant campylobacters in a multicenter collaborative trial.
A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.
Josefsen
M H
MH
Danish Institute for Food and Veterinary Research (DFVF), 27 Bülowsvej, DK-1790 Copenhagen V, Denmark.
Cook
N
N
D'Agostino
M
M
Hansen
F
F
Wagner
M
M
Demnerova
K
K
Heuvelink
A E
AE
Tassios
P T
PT
Lindmark
H
H
Kmet
V
V
Barbanera
M
M
Fach
P
P
Loncarevic
S
S
Hoorfar
J
J
D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002645:Chickens; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine