title: Acute vagus nerve stimulation does not suppress spike and wave discharges in genetic absence epilepsy rats from Strasbourg.
abstract: We evaluated the efficacy of vagus nerve stimulation (VNS) in Genetic Absence Epilepsy Rats from Strasbourg (GAERS), a validated model for absence epilepsy. In the first experiment, we investigated whether VNS applied at seizure onset can interrupt spike and wave discharges (SWD). In the second experiment, we investigated whether SWD are suppressed or shortened in duration when VNS is applied several hours per day. Both control and VNS groups underwent EEG and VNS electrode implantation. For the first experiment, a randomized crossover design was used. Stimuli (amplitude: 3 V; frequency: 30 Hz; pulse duration: 500 micros) were given when an SWD occurred on the EEG. The experiment was repeated the next day. In the second experiment, treated animals were stimulated (amplitude: 1.5 mA; frequency: 30 Hz; pulse duration: 500 micros; on/off time cycle: 30 s / 5 min) for 3h per day, during five consecutive days. In the first experiment, the duration of the SWD was increased on day 1, (P < 0.05). There was no difference in SWD duration on Day 2. In the second experiment, no significant differences could be found in number, duration and EEG frequency of SWD. VNS applied at the onset of an SWD can prolong the duration of SWD in GAERS. As a 5-day stimulation protocol had no effect, long-term VNS might be necessary to affect SWD.
authors.affiliation: Reference Centre for Refractory Epilepsy and Laboratory for Clinical and Experimental Neurophysiology, Department of Neurology, Ghent University Hospital, Ghent, Belgium. Stefanie.Dedeurwaerdere@UGent.be
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dedeurwaerdere
authors.forename: Kristl
authors.initials: K
authors.lastname: Vonck
authors.forename: Pieter
authors.initials: P
authors.lastname: Claeys
authors.forename: Peter
authors.initials: P
authors.lastname: Van Hese
authors.forename: Michel
authors.initials: M
authors.lastname: D'Havé
authors.forename: Thierry
authors.initials: T
authors.lastname: Grisar
authors.forename: Dean
authors.initials: D
authors.lastname: Naritoku
authors.forename: Paul
authors.initials: P
authors.lastname: Boon
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D004195:Disease Models, Animal; D004599:Electric Stimulation Therapy / Q000379:methods*; D004832:Epilepsy, Absence / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy*; D005260:Female; D008297:Male; D051381:Rats; D014630:Vagus Nerve / Q000502:physiology*