title: Expression patterns of the coe/ebf transcription factor genes during chicken and mouse limb development.
abstract: The COE (Collier/Olf/EBF) family of transcription factors comprises a single member in Drosophila and four members in human and mice. We have examined by in situ hybridization the expression patterns of each ebf/coe gene during limb development in mouse and chicken embryos. Expression of mouse ebf1, 2 and 3 is detected in mesenchymal cells from stages E10.5-11, expression of ebf2 being restricted to the presumptive zeugopod. Cross sections of mouse and chicken limb buds at several stages reveal that ebfs are specifically expressed in the connective tissues surrounding chondrogenic condensations and forming tendons. They thus represent useful new markers for studying vertebrate limb development, particularly formation of ligaments.
authors.affiliation: Centre de Biologie du Développement, UMR 5547 and IFR 109 CNRS/UPS, 118 route de Narbonne 31062 Toulouse cedex 4, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mella
authors.forename: Cathy
authors.initials: C
authors.lastname: Soula
authors.forename: Dominique
authors.initials: D
authors.lastname: Morello
authors.forename: Michèle
authors.initials: M
authors.lastname: Crozatier
authors.forename: Alain
authors.initials: A
authors.lastname: Vincent
mesh_terms: D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003238:Connective Tissue / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice / Q000196:embryology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D014157:Transcription Factors