title: Influence of osteoprotegerin (OPG) on resorption of heterotopically induced ossicle.
abstract: In this paper, the effect of osteoprotegerin (OPG) on slowing down the resorption process of heterotopically induced bone tissue is described. The induced ossicle is resorbed ex inactivitate. This system mimics osteoporosis in immobilised skeletal bones. Bone induction was achieved in BALB/c mice after the injection of the suspension of 3 x 10(6) HeLa cells into thigh muscle of animals immuno-suppressed by a single dose of hydrocortisone. To slow down the process of resorption we applied OPG and measured quantitatively the effect by weighing the mass of mineral deposited in the induced ossicle after hydrolysis of soft tissues surrounding the induced ossicles. As the effect of application of OPG more than 340-540% of bone mineral is found in the induced ossicles following nine applications of 0.05 mg OPG per mouse, every second day--in comparison to the control animals.
authors.affiliation: Institute for Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Kochanowska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Włodarski
authors.forename: Marek
authors.initials: M
authors.lastname: Pienkowski
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ostrowski
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000473:pathology; D017690:Cell Transplantation; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis; D010024:Osteoporosis / Q000188:drug therapy / Q000473:pathology; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; D014183:Transplantation, Heterologous
chemical_list: D006023:Glycoproteins; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; C506031:Tnfrsf11b protein, mouse