Presence of translation elongation factor-1A (eEF1A) in the excitatory postsynaptic density of rat cerebral cortex.
The postsynaptic density (PSD) is a proteinaceous cellular structure that is specialized for postsynaptic signal transduction. Here, we show that eukaryotic translation factor-1A (eEF1A; formerly known as eEF-1alpha) is associated with the excitatory PSD in rat forebrain. Immunoblot analysis showed that eEF1A in the PSD fraction is enriched over homogenate. Salt (1.0M NaCl), but not non-ionic detergents such as Triton X-100 (1.0%) and n-octyl glucoside (1.0%), could dissociate eEF1A from the PSD core. In cultured cortical neurons, eEF1A was colocalized with postsynaptic markers (PSD95 and SynGAPalpha), but not with a presynaptic marker (synaptophysin). These results indicate that eEF1A is present in the PSD of the excitatory synapses.
Cho
Sun-Jung
SJ
Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea.
Jung
Jae-Seob
JS
Ko
Bok Hyun
BH
Jin
IngNyol
I
Moon
Il Soo
IS
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D015151:Immunoblotting; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism
D020648:Peptide Elongation Factor 1