Evaluation of mouse blastocyst implantation rate by morphology grading.
The aim of our study is to observe the relationship between the blastocyst morphology and the implantation rate for mice. Mouse embryos obtained from the superovulated-ICR mice were cultured in vitro from 1-cell zygotes to blastocysts. Mouse blastocysts were then classified into 3 grades: grade I, small blastocysts; grade II, large blastocysts; grade III, hatching blastocysts. They were independently transferred into the uterus of recipient females mated with vasectomized male mice on 96 hours after the zygotes were cultured in vitro. The successful implantation was checked by injection of Chicago Sky Blue 6B on the second day after embryo transfer. Although there was no significant difference in the implantation rates between the grade III and grade II, grade I was significantly decreased, as compared with grade III. Grade I and grade II was also significantly decreased in both the diameter of blastocysts and cell number of inner cell mass (ICM) and trophectoderm (TE), as compared with grade III. These findings indicate that the expanded and hatching blastocyst selections for embryo transfer in in vitro fertilization were evaluated with the high implantation rate.
Cheng
Tzu-Chun
TC
Institute of Biochemistry, Medical College, Chung-Shan Medical University, Taichung, Taiwan, ROC.
Huang
Chun-Chia
CC
Huang
Lii-Sheng
LS
Chen
Chung-I
CI
Lee
Maw-Sheng
MS
Liu
Jer-Yuh
JY
D000818:Animals; D001391:Azo Compounds; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology*; D002452:Cell Count; D004396:Coloring Agents; D046508:Culture Techniques; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013194:Staining and Labeling; D013997:Time Factors; D014343:Trypan Blue; D014659:Vasectomy
D001391:Azo Compounds; D004396:Coloring Agents; C009000:pontamine sky blue; D014343:Trypan Blue