Endometrial cells derived from donor stem cells in bone marrow transplant recipients.
BACKGROUND
Regeneration of the endometrium in each menstrual cycle is required for reproduction. Endogenous endometrial stem cells reside in the basalis layer and serve as a source of cells that differentiate to form the endometrium. Bone marrow-derived cells have been shown to take on functions outside the hematopoietic system.


OBJECTIVE
To investigate the possibility that cells of extrauterine origin could repopulate the endometrium.


METHODS
Endometrium from 4 HLA-mismatched bone marrow transplant recipients (1998-2002) was evaluated for donor HLA expression. Each recipient had a bone marrow donor with an HLA type that enabled determination of the origin of any cell. Endometrial biopsies also were obtained from 4 healthy control women.


METHODS
HLA type was determined by immunohistochemistry and by reverse transcription-polymerase chain reaction.


RESULTS
Donor-derived endometrial cells were detected in endometrial biopsy samples from all bone marrow recipients and accounted for 0.2% to 48% of epithelial cells and 0.3% to 52% of stromal cells. None of the controls demonstrated HLA mismatch in endometrial samples.


CONCLUSIONS
These findings demonstrate that endometrial cells can originate from donor-derived bone marrow cells and suggest that nonuterine stem cells contribute to the regeneration of endometrial tissue.
Taylor
Hugh S
HS
Division of Reproductive Endocrinology, Yale University School of Medicine, New Haven, Conn 06520-8063, USA. hugh.taylor@yale.edu
D000328:Adult; D016026:Bone Marrow Transplantation / Q000473:pathology; D002454:Cell Differentiation; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells; D005260:Female; D006680:HLA Antigens; D006648:Histocompatibility; D006650:Histocompatibility Testing; D006801:Humans; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells; D014019:Tissue Donors; D014184:Transplantation, Homologous
D006680:HLA Antigens