Establishment and characterization of a human gastric carcinoma cell line TMC-1.
Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61%, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and beta-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53. The cell had the deletion at chromosome 18q and gains at chromosome 2p13-25, 5p15, 5q21-35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-alpha (2,000 U/ml) and by interferon-gamma with an IC50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-alpha and interferon-gamma. It can be used to develop new modalities of human gastric cancer treatment.
Shyu
Rong-Yaun
RY
Department of Internal Medicine, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Jiang
Shun-Yuan
SY
Jong
Yiin-Jeng
YJ
Cheng
Kuo-Chen
KC
Lin
Chi-Hung
CH
Yu
Jyh-Cherng
JC
Wu
Ming-Fang
MF
Chang
Tzu-Ming
TM
D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D018395:CA-19-9 Antigen / Q000096:biosynthesis; D015820:Cadherins / Q000096:biosynthesis; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D004171:Diploidy; D005260:Female; D016158:Genes, p53; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007621:Karyotyping; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis; D051176:beta Catenin
D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; C495270:CTNNB1 protein, human; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D016898:Interferon-alpha; D015534:Trans-Activators; D051176:beta Catenin; D007371:Interferon-gamma