Identification of the regulatory proteins in human pancreatic cancers treated with Trichostatin A by 2D-PAGE maps and multivariate statistical analysis.
In this paper, principal component analysis (PCA) is applied to a spot quantity dataset comprising 435 spots detected in 18 samples belonging to two different cell lines (Paca44 and T3M4) of control (untreated) and drug-treated pancreatic ductal carcinoma cells. The aim of the study was the identification of the differences occurring between the proteomic patterns of the two investigated cell lines and the evaluation of the effect of the drug Trichostatin A on the protein content of the cells. PCA turned out to be a successful tool for the identification of the classes of samples present in the dataset. Moreover, the loadings analysis allowed the identification of the differentially expressed spots, which characterise each group of samples. The treatment of both the cell lines with Trichostatin A therefore showed an appreciable effect on the proteomic pattern of the treated samples. Identification of some of the most relevant spots was also performed by mass spectrometry.
Marengo
Emilio
E
Department of Environmental and Life Sciences, University of Eastern Piedmont, Spalto Marengo 33, 15100 Alessandria, Italy. emilio.marengo@mfn.unipmn.it
Robotti
Elisa
E
Cecconi
Daniela
D
Hamdan
Mahmoud
M
Scarpa
Aldo
A
Righetti
Pier Giorgio
PG
D000704:Analysis of Variance; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D006877:Hydroxamic Acids / Q000627:therapeutic use*; D015999:Multivariate Analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000188:drug therapy* / Q000378:metabolism; D025341:Principal Component Analysis / Q000379:methods; D040901:Proteomics; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D000970:Antineoplastic Agents; D006877:Hydroxamic Acids; D009363:Neoplasm Proteins; C012589:trichostatin A