title: Haematogones in the peripheral blood of adults: a four-colour flow cytometry study of 102 patients.
abstract: Haematogones have been extensively characterized in bone marrow, but not in the peripheral blood (PB). We studied 102 PB samples from adult patients with a sensitive flow cytometry method. Sixty-six of 102 samples (65%) contained detectable haematogones, ranging from 0.01% to 1.3% of white blood cells (median 0.06%, mean 0.13%). Of 66 cases with complete blood count data, 51 had absolute haematogone counts of 0.00037-0.105 x 10(9)/l (median 0.0054 x 10(9)/l, mean 0.012 x 10(9)/l). PB haematogones belonged exclusively to the most mature maturational stage. These findings have implications for PB analysis of minimal residual disease in acute lymphoblastic leukaemia and follicular lymphoma.
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USA. kroft.steven@pathology.swmed.edu
authors.forename: Steven H
authors.initials: SH
authors.lastname: Kroft
authors.forename: Sheryl L
authors.initials: SL
authors.lastname: Asplund
authors.forename: Robert W
authors.initials: RW
authors.lastname: McKenna
authors.forename: Nitin J
authors.initials: NJ
authors.lastname: Karandikar
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018951:Antigens, CD20 / Q000032:analysis; D001402:B-Lymphocytes; D001772:Blood Cell Count; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D007958:Leukocyte Count; D008224:Lymphoma, Follicular / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000276:immunology; D015260:Neprilysin / Q000032:analysis; D013234:Stem Cells
chemical_list: D018951:Antigens, CD20; D015260:Neprilysin