Expression in bladder transitional cell carcinoma by real-time quantitative reverse transcription polymerase chain reaction array of 65 genes at the tumor suppressor locus 9q34.1-2: identification of 5 candidates tumor suppressor genes.
Frequent deletions on 9q34.1-2 were reported in bladder transitional cell carcinoma. High deletion mapping studies delimited a critical interval between markers D9S61 and D9S66, which is highly susceptible to contain a tumor suppressor gene. Expression level of the 65 genes localized in this region was analyzed by real-time quantitative RT-PCR, comparing tumor to normal urothelium. Five genes exhibited a significantly reduced expression level: C9orf9, KIAA0625, ABL1, LAMC3 and KIAA1857-netrin-G2, which exhibited the most significant downregulation (p=0.0007). KIAA1857-netrin-G2 belongs to the netrins and might then be a tumor suppressor gene in bladder cancer, as netrin1 receptor DCC has been implicated in tumorigenesis.
Amira
Najla
N
UroGene, Génopole, Evry, France.
Cancel-Tassin
Géraldine
G
Bernardini
Stéphane
S
Cochand-Priollet
Béatrix
B
Bittard
Hugues
H
Mangin
Philippe
P
Fournier
Georges
G
Latil
Alain
A
Cussenot
Olivier
O
D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000235:genetics*