title: The effect of specific gravity of perfluorocarbon liquid on the retina after experimental vitreous substitution.
abstract: OBJECTIVE
  To evaluate the histological changes in the retina after experimental vitreous substitution with various amounts of perfluorophenanthrene (PFPH).
  
  
  METHODS
  Thirty-two rabbit eyes were mechanically vitrectomized and filled up with 0.8 cc or 0.2 cc highly purified PFPH. The substance remained for 1 week (4 eyes), 2 weeks (8 eyes), 4 weeks (10 eyes) or 8 weeks (10 eyes). Eight eyes underwent the same surgical procedure without PFPH exchange to serve as a control group. A histological comparison of corresponding areas in the center and in the periphery of the inferior retina ensued.
  
  
  RESULTS
  After 2 weeks, nuclear drop-downs and irregularities of the outer plexiform layer and of both nuclear layers were observed centrally in the eyes with a 0.8 cc substitution. The changes proceeded to irregularities and cell loss of all retinal layers with focal areas of complete destruction of the retinal architecture after 8 weeks. In contrast, single nuclear drop-downs, wrinkling of the outer nuclear layer and cell loss in the photoreceptor layer were observed in the peripheral retina at the end of the observation period. In those eyes where 0.2 cc PFPH was exchanged nuclear drop-downs were found after 2 weeks, leading to focal thinning of the outer plexiform layer and irregularities of the outer nuclear layer after 4 weeks with an insignificant increase after 8 weeks. At this time these histological alterations were comparable with those that we observed after 2 weeks in the eyes with a 0.8 cc tamponade in a corresponding area.
  
  
  CONCLUSIONS
  Even high purification of PFPH does not prevent retinal damage. The different results in different areas after vitreous substitution with 0.2 cc and with 0.8 cc demonstrate that the high specific gravity of the substance may also play a role in the development of histological changes after extended tamponade.
authors.affiliation: Ludwig Boltzmann Institute of Retinology and Biomicroscopic Laser Surgery, Department of Ophthalmology, Krankenanstalt Rudolfstiftung, Juchgasse 25, 1030, Vienna, Austria. ulrike.stolba@kar.magwien.gv.at
authors.forename: Ulrike
authors.initials: U
authors.lastname: Stolba
authors.forename: Katharina
authors.initials: K
authors.lastname: Krepler
authors.forename: Michaela
authors.initials: M
authors.lastname: Velikay-Parel
authors.forename: Susanne
authors.initials: S
authors.lastname: Binder
mesh_terms: D000818:Animals; D005466:Fluorocarbons / Q000633:toxicity*; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D013046:Specific Gravity; D014821:Vitrectomy
chemical_list: D005466:Fluorocarbons; C047722:perfluorophenanthrene