[Experimental study of the expression of the bladder wall proteins in bladder outlet obstruction in the rabbit].
OBJECTIVE
To determine the relationship of the bladder wall levels of the contractile proteins: whole actin, beta-actin, alpha-sarcomeric-actina caldesmon and of the Heat Stress Protein HSP-70, with the partial bladder outlet obstruction.


METHODS
It was carried out an experimental study on 43 New-Zealand rabbits. These animals were divided into a control group and six experimental groups with partial bladder outlet obstruction induced by an incomplete tie around the urethra. The experimental groups were defined according the day when the animals were sacrified: at 24 hours, 7 days, 15 days, 1 month, 3 months and 6 months of the obstruction. It was determined in all animals the muscular bladder wall concentration of whole actin, beta-actin, alpha-sarcomeric-actin, caldesmon and HSP-70 protein, according to Western Blotting technique. The results were analyzed by means of ANOVA, using the Scheffe post hoc method.


RESULTS
The bladder weight of the obstructed animals increased significantly in two stages: until the day 15 and between the day 15 and the end of the study, corresponding to theoretical phases of initial hypertrophy and compensation. The level of whole actin showed a significant decrease at the beginning of compensation phase, while the beta-actin level increased in the phase of initial hypertrophy and the alpha-sarcomeric-actin level increased at the end of the compensation phase, while the caldesmon and the HSP-70 made it at the end of the initial hypertrophy phase.


CONCLUSIONS
Analysing our data we conclued that the determination of the caldesmon levels is the marker that better adjusts to the changes associated with partial bladder outlet obstruction in the rabbit.
Salinas Casado
J
J
Servicio de Urología, Hospital Clínico San Carlos, Universidad Complutense, Madrid.
Virseda Chamorro
M
M
Martín Vega
A
A
Hernández Lao
A
A
Herrero Payo
J A
JA
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D008297:Male; D011817:Rabbits; D013997:Time Factors; D001743:Urinary Bladder / Q000378:metabolism*; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism*
D000199:Actins; D002148:Calmodulin-Binding Proteins; D018840:HSP70 Heat-Shock Proteins