Detection of Amadori compounds by capillary electrophoresis coupled to tandem mass spectrometry.
Capillary electrophoresis coupled to mass spectrometry (CE-MS) is reported for the first time as an alternative and powerful analytical method for the characterization and monitoring of N-substituted 1-amino-1-deoxyketoses (Amadori compounds). It allows rapid separation and identification of Amadori compounds, while benefiting from the well-known advantages of MS, such as specificity and sensitivity. Amadori compounds of several amino acids, such as glycine, valine, isoleucine, methionine, proline, and phenylalanine, as well as a cysteine-derived compound, were separated and/or discriminated using CE-MS/MS under standard conditions. The technique may also be useful to study the stability and degradation kinetics of other labile charged Maillard intermediates that play an important role in food and medical science.
Hau
Jörg
J
Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
Devaud
Stéphanie
S
Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
Blank
Imre
I
Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D005502:Food; D005947:Glucose / Q000737:chemistry; D006358:Hot Temperature; D007661:Ketoses / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D015416:Maillard Reaction; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis
D000596:Amino Acids; D007661:Ketoses; D004364:Pharmaceutical Preparations; D005947:Glucose