Lipid peroxidation and antioxidant systems in the blood of young rats subjected to chronic fluoride toxicity.
Wistar albino rats were exposed to 30 or 100 ppm fluoride in drinking water during their fetal, weanling and post-weaning stages of life up to puberty. Extent of lipid peroxidation and response of the antioxidant systems in red blood cells and plasma to prolonged fluoride exposure were assessed in these rats in comparison to the control rats fed with permissible level (0.5 ppm) of fluoride. Rats treated with 100 ppm fluoride showed enhanced lipid peroxidation as evidenced by elevated malondialdehyde (MDA) levels in red blood cells but, 30 ppm fluoride did not cause any appreciable change in RBC MDA level. 30 ppm fluoride-intake resulted in increased levels of total and reduced glutathione in red blood cells and ascorbic acid in plasma while 100 ppm fluoride resulted in decreases in these levels. The activity of RBC glutathione peroxidase was elevated in both the fluoride-treated groups, more pronounced increase was seen with 100 ppm. Reduced to total glutathione ratio in RBC and uric acid levels in plasma decreased in both the groups. RBC superoxide dismutase activity decreased significantly on high-fluoride treatment. These results suggest that long-term high-fluoride intake at the early developing stages of life enhances oxidative stress in the blood, thereby disturbing the antioxidant defense of rats. Increased oxidative stress could be one of the mediating factors in the pathogenesis of toxic manifestations of fluoride.
Shivarajashankara
Y M
YM
Department of Biochemistry, M.R. Medical College, Gulbarga 585105, India. shivrajsym@yahoo.com
Shivashankara
A R
AR
Bhat
P Gopalakrishna
PG
Rao
S Hanumanth
SH
D000284:Administration, Oral; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000097:blood; D014527:Uric Acid / Q000097:blood
D014527:Uric Acid; D008315:Malondialdehyde; D012969:Sodium Fluoride; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid