title: Chronic lymphocytic leukemia in the course of chronic myelocytic leukemia: evidence of independent clonal origin as shown by interphase fluorescence in situ hybridization and fluorescence-activated cell sorting.
abstract: We report the case of a 57-year-old man who developed chronic lymphocytic leukemia (CLL) several months after the initial diagnosis of Philadelphia (Ph) chromosome-positive (Ph(+)) chronic myelocytic leukemia. CLL cells were purified by using fluorescence-activated cell sorting and further analyzed using interphase fluorescence in situ hybridization with probes to detect the BCR/ABL fusion gene. We provide evidence that the CLL cells arose in a Ph(-) clone.
authors.affiliation: Department of Laboratory Hematology, Princess Margaret Hospital-University Health Network, University of Toronto, 610 University Ave., Toronto, ON M5G 2M9, Canada. hong.chang@uhn.on.ca
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.forename: Rob
authors.initials: R
authors.lastname: Sutherland
authors.forename: Rakash
authors.initials: R
authors.lastname: Nayar
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Suzane
authors.initials: S
authors.lastname: Kamel-Reid
authors.forename: Mary-Anne
authors.initials: MA
authors.lastname: Mile
authors.forename: Hans
authors.initials: H
authors.lastname: Messner
authors.forename: Jeff
authors.initials: J
authors.lastname: Lipton
mesh_terms: D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl / Q000032:analysis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D054438:Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome
chemical_list: D016044:Fusion Proteins, bcr-abl