title: Vascular mural cells in healing canine myocardial infarcts.
abstract: Angiogenesis is a critical process in healing of myocardial infarcts, leading to the formation of highly vascular granulation tissue. However, effective cardiac repair depends on mechanisms that inhibit the angiogenic process after a mature scar is formed, preventing inappropriate expansion of the fibrotic process. Using a canine model of reperfused myocardial infarction, we demonstrated that maturation of the infarct leads to the formation of neovessels, with a thick muscular coat, that demonstrate distinct morphological characteristics. Many of these "neoarterioles" lack a defined internal elastic lamina and demonstrate irregular deposits of extracellular matrix in the media. Vascular mural cells in healing infarcts undergo phenotypic changes, showing minimal expression of desmin during the proliferative phase (1 hr occlusion/7 days reperfusion) but in the mature scar (8 weeks reperfusion) acquire a phenotype similar to that of vascular smooth muscle cells in control areas. Non-muscle myosin heavy chains A and B are induced in infarct endothelial cells and myofibroblasts, respectively, but are not expressed in neovascular mural cells. Recruitment of a muscular coat and formation of neoarterioles in mature scars may inhibit endothelial cell proliferation and vascular sprouting, stabilizing the infarct vasculature.
authors.affiliation: Section of Cardiovascular Sciences, One Baylor Plaza M/S F-602, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Marcin
authors.initials: M
authors.lastname: Dobaczewski
authors.forename: Spyridon
authors.initials: S
authors.lastname: Akrivakis
authors.forename: Kamal
authors.initials: K
authors.lastname: Nasser
authors.forename: Lloyd H
authors.initials: LH
authors.lastname: Michael
authors.forename: Mark L
authors.initials: ML
authors.lastname: Entman
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Frangogiannis
mesh_terms: D000818:Animals; D001160:Arterioles / Q000473:pathology; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D009203:Myocardial Infarction / Q000473:pathology*; D018995:Myosin Heavy Chains / Q000096:biosynthesis; D018919:Neovascularization, Physiologic; D010641:Phenotype; D020033:Protein Isoforms / Q000096:biosynthesis
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains