Generation and characterization of monoclonal antibodies to protective antigen of Bacillus anthracis.
Monoclonal antibodies (MoAbs) were generated following immunization of BALB/c mice with protective antigen (PA) of B. anthracis. Five clones reactive to this protein were stabilized and preserved. These MoAbs could detect nanogram levels of PA when tested in ELISA. In Western blotting, they reacted with all PA preparations tested and no cross-reactivity was observed with lethal factor, edema factor of B. anthracis and with other organisms. These MoAbs could detect PA from 22 confirmed clinical isolates of B. anthracis on Western blotting and hold promise for direct detection of PA in clinical samples for diagnosing anthrax.
Sastry
K S R
KS
Division of Microbiology, Defence R & D Establishment, Gwalior 474 002, India. ksrsastry2001@yahoo.com
Tuteja
U
U
Batra
H V
HV
D000818:Animals; D000881:Anthrax / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001408:Bacillus anthracis / Q000276:immunology*; D001427:Bacterial Toxins; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D014757:Viper Venoms / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002352:Carrier Proteins; D014757:Viper Venoms; C030325:anthrax toxin; C416405:edema factor