title: Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival.
abstract: CD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.
authors.affiliation: Laboratory Medicine Academic Group, The Medical School, University of Manchester, Manchester, M13 9PT, UK. baoqiang.guo@man.ac.uk
authors.forename: Baoqiang
authors.initials: B
authors.lastname: Guo
authors.forename: Paul
authors.initials: P
authors.lastname: Rooney
authors.forename: Mark
authors.initials: M
authors.lastname: Slevin
authors.forename: Chenggang
authors.initials: C
authors.lastname: Li
authors.forename: Sudeep
authors.initials: S
authors.lastname: Parameshwar
authors.forename: Donghui
authors.initials: D
authors.lastname: Liu
authors.forename: Pat
authors.initials: P
authors.lastname: Kumar
authors.forename: Carmelo
authors.initials: C
authors.lastname: Bernabeu
authors.forename: Shant
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002448:Cell Adhesion; D002470:Cell Survival; D000071063:Endoglin; D015870:Gene Expression; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis / Q000235:genetics; D048055:Mitogen-Activated Protein Kinase 8 / Q000235:genetics / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D051381:Rats; D011956:Receptors, Cell Surface; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019012:Integrin beta1; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D048055:Mitogen-Activated Protein Kinase 8