Human schistosomiasis mansoni: intensity of infection differentially affects the production of interleukin-10, interferon-gamma and interleukin-13 by soluble egg antigen or adult worm antigen stimulated cultures.
The effect of the intensity of infection (eggs per gram faeces, epg) on the production of interferon-gamma (INF-gamma), interleukin (IL)-10 and IL-13 by peripheral blood mononuclear cells (PBMCs) from individuals living in a Schistosoma mansoni-endemic area was evaluated. In vitro stimulation of PBMCs with soluble egg antigen (SEA) resulted in significantly higher secretion levels of IFN-gamma in egg-negative individuals compared with those with an intensity of infection of more than 100 epg. In contrast, the egg-positive group produced significantly higher amounts of IL-10. Levels of IL-13 did not differ significantly between egg-positive and egg-negative groups. These findings suggest that IL-10 is an important cytokine in the control of the T helper cell (Th) type 1 responses during human S. mansoni infection, shifting the immune response from Th0 in egg-negative individuals from an endemic area to a Th2 polarization in chronic infected individuals.
Silveira
Alda M S
AM
Laboratório de Imunologia, Faculdade de Ciências da Saúde. Universidade Vale do Rio Doce, Governador Valadares, Brazil.
Gazzinelli
Giovanni
G
Alves-Oliveira
Lúcia F
LF
Bethony
Jeffrey
J
Gazzinelli
Andrea
A
Carvalho-Queiroz
Claudia
C
Alvarez
Maria Carolina B
MC
Lima-Silva
Francisco C
FC
Prata
Aluizio
A
LoVerde
Philip T
PT
Correa-Oliveira
Rodrigo
R
D000328:Adult; D000367:Age Factors; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001938:Brazil / Q000453:epidemiology; D002478:Cells, Cultured; D004198:Disease Susceptibility / Q000276:immunology; D019353:Endemic Diseases; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000276:immunology; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000453:epidemiology / Q000276:immunology*; D012720:Severity of Illness Index
D000947:Antigens, Helminth; D018793:Interleukin-13; D016753:Interleukin-10; D007371:Interferon-gamma