title: Detection of Amadori compounds by capillary electrophoresis coupled to tandem mass spectrometry.
abstract: Capillary electrophoresis coupled to mass spectrometry (CE-MS) is reported for the first time as an alternative and powerful analytical method for the characterization and monitoring of N-substituted 1-amino-1-deoxyketoses (Amadori compounds). It allows rapid separation and identification of Amadori compounds, while benefiting from the well-known advantages of MS, such as specificity and sensitivity. Amadori compounds of several amino acids, such as glycine, valine, isoleucine, methionine, proline, and phenylalanine, as well as a cysteine-derived compound, were separated and/or discriminated using CE-MS/MS under standard conditions. The technique may also be useful to study the stability and degradation kinetics of other labile charged Maillard intermediates that play an important role in food and medical science.
authors.affiliation: Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hau
authors.affiliation: Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Devaud
authors.affiliation: Nestec Ltd., Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Imre
authors.initials: I
authors.lastname: Blank
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D005502:Food; D005947:Glucose / Q000737:chemistry; D006358:Hot Temperature; D007661:Ketoses / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D015416:Maillard Reaction; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis
chemical_list: D000596:Amino Acids; D007661:Ketoses; D004364:Pharmaceutical Preparations; D005947:Glucose