title: Functional assessment of cryopreserved human aortas for pharmaceutical research.
abstract: We evaluated the impact of standard cryopreservation on functional properties of human aortic homografts. From seven human donors, the thoracic descending aorta was obtained. Effects of cryopreservation on contractibility and endothelium function were tested. After cryopreservation no endothelium-dependent or endothelium-independent relaxation was found and the contractibility was strongly affected. Arteries showed no function and loss of endothelial integrity after cryopreservation and thawing.
authors.affiliation: Cryobiology Unit, Complejo Hospitalario Juan Canalejo, La Coruña, Spain. Esther_Rendal@canalejo.cesga.es
authors.forename: Maria Esther
authors.initials: ME
authors.lastname: Rendal Vázquez
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez Cabarcos
authors.forename: M V
authors.initials: MV
authors.lastname: Martínez Santos
authors.forename: R O
authors.initials: RO
authors.lastname: Fernández Mallo
authors.forename: J
authors.initials: J
authors.lastname: Sánchez Ibáñez
authors.forename: R
authors.initials: R
authors.lastname: Segura Iglesias
authors.forename: G
authors.initials: G
authors.lastname: Matheu Capó
authors.forename: P
authors.initials: P
authors.lastname: Filgueira Fernández
authors.forename: S
authors.initials: S
authors.lastname: Pértega Díaz
authors.forename: T
authors.initials: T
authors.lastname: Bermúdez González
authors.forename: C
authors.initials: C
authors.lastname: Andión Núñez
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009599:Nitroprusside / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D014021:Tissue Preservation; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D009599:Nitroprusside; D000109:Acetylcholine; D009638:Norepinephrine