title: Applications of murine and humanized chimaeric monoclonal antibodies for red cell phenotyping.
abstract: The limited supply of reagent human polyclonal antibodies to high prevalence antigens, like Js(b), is driving the search for alternative reagents. Murine immunoglobulin G (IgG) monoclonal antibodies (Mabs) and their humanized chimaeric IgM isoforms can now be used for typing patients and screening donors. Antigen typing of red blood cells (RBC) with a positive direct antiglobulin test (DAT) is also possible using these antibodies. Blood from patients with sickle cell disease and African donors were tested with reagent anti-Js(b), murine Mab IgG anti-Js(b) [murine immunochemistry monoclonal antibody-8 (MIMA-8)], and humanized chimaeric IgM anti-Js(b) [human immunochemistry monoclonal antibody-8 (HIMA-8)] by haemagglutination and gel cards. RBC samples that were DAT positive were used to evaluate the humanized chimaeric IgM monoclonal anti-Fy(a) (HIMA-19). RBC samples (n = 243) of known Js(b) type were tested in parallel with MIMA-8 and reagent anti-Js(b), and 132 samples were tested with MIMA-8 in gel cards and HIMA-8 by direct tube testing. No discrepant results were obtained. DAT-positive RBC samples (n = 27) were correctly phenotyped using HIMA-19. We conclude that MIMA-8 is suitable for screening donors and typing patient RBCs. Testing MIMA-8 with gel cards containing anti-mouse IgG enables the screening of donors by automated methods. Humanized chimaeric IgM anti-Js(b) and anti-Fy(a) are suitable as typing reagents by direct agglutination methods.
authors.affiliation: National Blood Service, Colindale, UK.
authors.forename: E
authors.initials: E
authors.lastname: Lee
authors.forename: G
authors.initials: G
authors.lastname: Burgess
authors.forename: G R
authors.initials: GR
authors.lastname: Halverson
authors.forename: T J
authors.initials: TJ
authors.lastname: Huang
authors.forename: M E
authors.initials: ME
authors.lastname: Reid
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001788:Blood Grouping and Crossmatching; D002678:Chimera; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D010641:Phenotype; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic