Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy.
We describe a method for en face phase-contrast imaging of cells with a fiber-based differential phase-contrast optical coherence microscopy system. Recorded en face images are quantitative phase-contrast maps of cells due to spatial variation of the refractive index and (or) thickness of various cellular components. Quantitative phase-contrast images of human epithelial cheek cells obtained with the fiber-based differential phase-contrast optical coherence microscopy system are presented.
Rylander
Christopher G
CG
Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Davé
Digant P
DP
Akkin
Taner
T
Milner
Thomas E
TE
Diller
Kenneth R
KR
Welch
Ashley J
AJ
D002610:Cheek; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007368:Interferometry; D008858:Microscopy, Phase-Contrast; D009061:Mouth Mucosa / Q000166:cytology; D041623:Tomography, Optical Coherence