Characterization of a novel molluskan tyrosine kinase receptor that inhibits neurite regeneration.
Receptor tyrosine kinases play many important roles in neuronal signaling including regulating neurite outgrowth. We have identified a novel receptor tyrosine kinase, neurite outgrowth regulating kinase (nork) from Aplysia californica. A fragment of this kinase was also identified in another mollusk, Lymnaea. The kinase domain is equally homologous to the Ret (rearranged during transformation) and fibroblast growth factor receptor families, but the extracellular domain is entirely novel, suggesting that it binds a nonconserved ligand. Overexpression of neurite outgrowth regulating kinase, but not a kinase dead form, causes a reduction in neurite outgrowth of Aplysia sensory neurons. Thus, we have identified a novel receptor tyrosine kinase implicated in regulating neurite outgrowth.
Hislop
Jonathan
J
Department of Neurology and Neurosurgery, McGill University, Montreal Neurological Institute, Montreal, Quebec, Canada H3A-2B4.
Dyer
John R
JR
Scott
Darcy
D
van Kesteren
Ronald E
RE
Sossin
Wayne S
WS
D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001048:Aplysia / Q000235:genetics*; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008164:Luminescent Proteins / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D011233:Precipitin Tests / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment / Q000379:methods; D014162:Transfection / Q000379:methods
D000906:Antibodies; D008164:Luminescent Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D020794:Receptor Protein-Tyrosine Kinases