Proteomics in Drosophila melanogaster.
To be able to understand cellular mechanisms, we require fully integrated data sets combining information about gene expression, protein expression, post-translational modification states, sub-cellular location and complex formation. Proteomics is a very powerful technique that can be applied to interrogate changes at the protein level. Studying this effectively requires specialised facilities within research institutes. Here, we describe the setting up and operation of such a facility, providing a resource for the Arabidopsis and Drosophila research communities.
Lilley
Kathryn S
KS
Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge. k.s.lilley@bioc.cam.ac.uk
Griffiths
Delia R
DR
D000818:Animals; D030562:Databases, Protein; D004331:Drosophila melanogaster / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D013347:Subcellular Fractions
D020543:Proteome