title: [Biocompatibility testing of various biomaterials as dependent on immune status].
abstract: BACKGROUND
  This study deals with the ingrowth behaviour of biomaterials (hydroxyapatite, cp-titanium, cobalt-chromium-molybdenum and PAEK) in relationship to the immunological competence in an animal model. Measured were the production of extracellular matrix (ECM) after implantation in non-immunocompetent naked mice and immunocompetent wild mice. Intention of the trial was to find out if either the immunological competence or the duration of implantation influences the quantity of produced ECM. In addition, the ingrowth behaviour was investigated under these conditions by using four different biomaterials.
  
  
  METHODS
  Biomaterials (hydroxyapatite, cp-titanium, cobalt-chromium-molybdenum and PAEK) were implanted for 14 or 60 days, respectively. CLSM, SEM and SEM-EDX were used for analysis of the ECM and for measuring the distance between ECM and the biomaterials. CLSM was also used for the detection of collagen I and III as a parameter of the quality of osteointegration.
  
  
  RESULTS
  In all cases a matrix grew on the surface of the biomaterials. The CLSM detected a co-localisation of collagen I and III. In the case of hydroxyapatite collagen I and III were found at a distance of 1 micro m over the surface. The largest space between the surface of the implant and the ECM was found in the case of PAEK. The smallest space was in the case of hydroxyapatite. In all investigated biomaterials the proportion of collagen I to collagen III varied through the duration of implantation.
  
  
  CONCLUSIONS
  As is known from the literature we found different ingrowth behaviours on using different biomaterials. Furthermore, we found a statistically significant influence of the immunological competence of the host with regard to ECM production. We draw the conclusion that immunological competence improves the ingrowth behaviour of biomaterials.
authors.affiliation: Klinik für Orthopädie und Rheumatologie der Philipps-Universität Marburg.
authors.forename: S
authors.initials: S
authors.lastname: Endres
authors.forename: M
authors.initials: M
authors.lastname: Landgraff
authors.forename: M
authors.initials: M
authors.lastname: Kratz
authors.forename: A
authors.initials: A
authors.lastname: Wilke
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D018786:Bone Substitutes / Q000009:adverse effects*; D001842:Bone and Bones / Q000276:immunology* / Q000473:pathology / Q000601:surgery*; D005549:Foreign-Body Reaction / Q000209:etiology / Q000276:immunology* / Q000473:pathology*; D008422:Materials Testing / Q000379:methods; D051379:Mice; D016348:Osseointegration / Q000276:immunology*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes