Differential gene expression during pre-symbiotic interaction between Tuber borchii Vittad. and Tilia americana L.
Ectomycorrhizal formation is a highly regulated process involving the molecular reorganization of both partners during symbiosis. An analogous molecular process also occurs during the pre-symbiotic phase, when the partners exchange molecular signals in order to position and prepare both organisms for the establishment of symbiosis. To gain insight into genetic reorganization in Tuber borchii during its interaction with its symbiotic partner Tilia americana, we set up a culture system in which the mycelium interacts with the plant, even though there is no actual physical contact between the two organisms. The selected strategies, suppressive subtractive hybridisation and reverse Northern blots, allowed us to identify, for the first time, 58 cDNA clones differentially expressed in the pre-symbiotic phase. Sequence analysis of the expressed sequence tags showed that the expressed genes are involved in several biochemical pathways: secretion and apical growth, cellular detoxification, general metabolism and both mutualistic and symbiotic features.
Menotta
M
M
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Via Saffi 2, Urbino, Italy. mmenotta@uniurb.it
Amicucci
A
A
Sisti
D
D
Gioacchini
A M
AM
Stocchi
V
V
D001203:Ascomycota / Q000235:genetics* / Q000502:physiology; D015152:Blotting, Northern / Q000379:methods; D019295:Computational Biology; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D030562:Databases, Protein; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D025282:Mycelium / Q000502:physiology; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D032384:Tilia
D017931:DNA Primers; D018076:DNA, Complementary