title: Preischemic selenium status as a major determinant of myocardial infarct size in vivo in rats.
abstract: Prospective epidemiological studies have shown that the incidence of numerous cardiovascular pathologies is correlated with body selenium status. However, it remains unclear whether selenium status also influences the outcome of myocardial infarction. The aim of the present study was to test whether dietary selenium intake affects myocardial necrosis induced by transient regional ischemia in vivo in rats. For this purpose, male Wistar rats received either a high-selenium (High-Se: 1.5 mg of Se/kg) or a low-selenium (Low-Se: 0.05 mg of Se/kg) diet for 10 weeks. Animals were subjected to 30 min of myocardial ischemia induced by coronary artery ligation followed by 60 min of reperfusion. Pre- and postischemic blood samples were collected for glutathione (GSH and GSSG) determination and for glutathione peroxidase (GSH-Px) assessment. Our results show that high-selenium intake reduces myocardial infarct size (High-Se: 25.16 +/- 1.19% versus Low-Se: 36.51 +/- 4.14%, p < 0.05), preserves postischemic GSH/GSSG ratio (High-Se: 1.37 +/- 0.37 versus Low-Se: 0.47 +/- 0.10, p < 0.05), increases plasma GSH-Px activity, and improves postischemic mean arterial pressure. In conclusion, preischemic body selenium status is a major determinant of the outcome of myocardial ischemia in vivo in rats probably because it influences the cellular redox status.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Tanguy
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morel
authors.forename: Corinne
authors.initials: C
authors.lastname: Berthonneche
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Toufektsian
authors.forename: Michel
authors.initials: M
authors.lastname: de Lorgeril
authors.forename: Véronique
authors.initials: V
authors.lastname: Ducros
authors.forename: Arpad
authors.initials: A
authors.lastname: Tosaki
authors.forename: Joel
authors.initials: J
authors.lastname: de Leiris
authors.forename: François
authors.initials: F
authors.lastname: Boucher
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004032:Diet; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology*; D017202:Myocardial Ischemia / Q000378:metabolism*; D010084:Oxidation-Reduction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000097:blood*
chemical_list: D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium