title: Pulsed-field electrophoretic fingerprinting of Salmonella indiana and its epidemiological applicability.
abstract: Eight Xba I-generated pulsed-field profile (PFP) types and four subtypes within one of the most common PFP types have been identified in Salmonella indiana from patients, poultry and human food in England and Wales in the three-year period from January 1994 to December 1996. Two PFP types have predominated, PFP X1 and PFP X2. Although the PFP X1 type was identified throughout the study period, the PFP X2 type was not identified until late 1995, subsequently becoming the most common PFP type in humans in the first six months of 1996 with a significant distribution in elderly patients. It is concluded that PFGE can be used in support of epidemiological investigations for the subdivision of Salm. indiana. Furthermore, as both conditions and interpretation criteria can be easily standardized, it is suggested that for many salmonella serotypes, PFGE can provide the basis for a definitive scheme of genotypic subtyping suitable for epidemiological investigations at both a national and international level.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: Punia
authors.forename: M D
authors.initials: MD
authors.lastname: Hampton
authors.forename: A M
authors.initials: AM
authors.lastname: Ridley
authors.forename: L R
authors.initials: LR
authors.lastname: Ward
authors.forename: B
authors.initials: B
authors.lastname: Rowe
authors.forename: E J
authors.initials: EJ
authors.lastname: Threlfall
mesh_terms: D000368:Aged; D000821:Animal Feed; D000818:Animals; D002645:Chickens; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004739:England / Q000453:epidemiology; D005516:Food Microbiology; D006801:Humans; D011200:Poultry; D015995:Prevalence; D012475:Salmonella / Q000235:genetics*; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D014852:Wales / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial