Fibroblast growth and H-7 protein kinase inhibitor response monitored in microimpedance sensor arrays.
Functional genomic studies and drug candidate testing both require high throughput, parallel experimentation strategies to screen for variable cellular behaviors. In this article we describe the use of an impedance sensing electrode array that is capable of sensing cell "presence" as well as the extent of cell (focal) attachment to the substrate. The signals provided by mouse fibroblasts on a sensing structure containing four different sized electrodes are reported. In the absence of cells, each electrode's impedance was found to depend as expected on electrode size and frequency. The impedance increased by several-fold when fibroblasts attached and spread out over time. More notably, the sensors also detected the cellular response to the protein kinase C inhibitor, H-7. H-7 inhibits actomyosin contractility; thereafter, the loss of focal adhesion complexes occurs. The sensors, in turn, detected an impedance decrease after H-7 addition and an increase in impedance after H-7 removal.
Nguyen
Duc D
DD
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Huang
Xiaoqiu
X
Greve
David W
DW
Domach
Michael M
MM
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D003198:Computer Simulation; D017097:Electric Impedance; D004594:Electrophysiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008839:Microelectrodes; D008856:Microscopy, Fluorescence; D022502:Stress Fibers / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D006046:Gold; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine