Intracellular localization of VDAC proteins in plants.
Voltage-dependent anion channels (VDACs) are porin-type beta-barrel diffusion pores. They are prominent in the outer membrane of mitochondria and facilitate metabolite exchange between the organelle and the cytosol. Here we studied the subcellular distribution of a plant VDAC-like protein between plastids and mitochondria in green and non-green tissue. Using in vitro studies of dual-import into mitochondria and chloroplasts as well as transient expression of fluorescence-labeled polypeptides, it could be clearly demonstrated that this VDAC isoform targets exclusively to mitochondria and not to plastids. Our results support the idea that plastids evolved a concept of solute exchange with the cytosol different from that of mitochondria.
Clausen
Cathrin
C
Department Biologie I, Ludwig-Maximilians-Universität München, Menzingerstr. 67, 80638 München, Germany.
Ilkavets
Iryna
I
Thomson
Rowena
R
Philippar
Katrin
K
Vojta
Aleksandar
A
Möhlmann
Torsten
T
Neuhaus
Ekkehard
E
Fulgosi
Hrvoje
H
Soll
Jürgen
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002458:Cell Fractionation; D002736:Chloroplasts / Q000737:chemistry; D016384:Consensus Sequence; D017931:DNA Primers; D008928:Mitochondria / Q000737:chemistry*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018272:Porins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000737:chemistry / Q000166:cytology; D050994:Voltage-Dependent Anion Channels
D017931:DNA Primers; D018272:Porins; D050994:Voltage-Dependent Anion Channels