The cytoprotective effect of inulin-type hexasaccharide extracted from Morinda officinalis on PC12 cells against the lesion induced by corticosterone.
High concentration of corticosterone (Cort) 0.2 mM was incubated with PC12 cells to simulate the lesion state of brain neurons in depressive illness, it was found that the inulin-type oligosaccharides extracted from Morinda officinalis, inulin-type hexasaccharide (IHS) at the doses of 0.625, 1.25 microM or desipramine (DIM) 0.25, 1 microM protected the PC12 cells from the lesion induced by Cort. With Fura-2/AM labeling assay, DIM 0.25, 1 microM or IHS 2.5, 10 microM attenuated the intracellular Ca2+ overloading induced by Cort 0.1 mM for 48 h in PC12 cells. Using RT-PCR, treatment with Cort 0.1 mM for 48 h decreased the nerve growth factor (NGF) mRNA level in PC12 cells, IHS 5, 10 microM reversed this change. In summary, IHS attenuate the intracellular Ca2+ overloading and thereby up-regulate the NGF mRNA expression in Cort-treated PC12 cells, which may be consisted at least part of the cytopretective effect of IHS. These results also extend evidence for our hypothesis that neuroprotective action is one of the common mechanisms for antidepressants.
Li
Yun-Feng
YF
Division of Psychopharmacology, Beijing Institute of Pharmacology and Toxicology, 27 TaiPing Road, Beijing 100850, China. lyf619@yahoo.com.cn
Liu
Yan-Qin
YQ
Yang
Ming
M
Wang
Heng-Lin
HL
Huang
Wen-Chao
WC
Zhao
Yi-Min
YM
Luo
Zhi-Pu
ZP
D000704:Analysis of Variance; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003345:Corticosterone / Q000037:antagonists & inhibitors*; D019610:Cytoprotection / Q000187:drug effects*; D017931:DNA Primers; D003891:Desipramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005470:Fluorometry; D016257:Fura-2; D005786:Gene Expression Regulation / Q000187:drug effects; D032066:Morinda / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009844:Oligosaccharides / Q000494:pharmacology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000929:Antidepressive Agents, Tricyclic; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D009844:Oligosaccharides; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D002118:Calcium; D003891:Desipramine; D016257:Fura-2; D003345:Corticosterone