title: Production of thiophenes from callus cultures of Tagetes patula L. and its mosquito larvicidal activity.
abstract: Calli were initiated from leaf tissues of T. patula, on Murashige and Skoog's medium supplemented with 2-4 dichlorophenoxacetic acid (2,4-D) and kinetin (Kn). The maximum accumulation of biomass was recorded on 6th week at 1.11 g. dry wt/culture. Maximum thiophene content was recorded on 4th week at 0.008% on dry wt basis in callus cultures of T. patula subjected to nutrient stress. Nitrogen stress induced 3-fold increase in thiophene production level in six weeks (0.024% on dry weight basis). The best hormonal supplementation required for thiophenes production was found to be 2,4-D (2.0 mg L(-1)) and kinetin (2.0 mg L(-1)). The thiophenes produced in callus cultures of T. patula showed larvicidal effect against mosquito larvae.
authors.affiliation: Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 013, India.
authors.forename: T
authors.initials: T
authors.lastname: Rajasekaran
authors.forename: G A
authors.initials: GA
authors.lastname: Ravishanakar
authors.forename: B Obul
authors.initials: BO
authors.lastname: Reddy
mesh_terms: D000818:Animals; D009033:Culicidae / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques; D007306:Insecticides; D007814:Larva / Q000187:drug effects*; D031686:Tagetes / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007306:Insecticides; D013876:Thiophenes