[Effect of exogenous pulmonary surfactant on isolated lung injury induced by ischemia-reperfusion in rats].
OBJECTIVE
To study the effect of exogenous pulmonary surfactant (PS) on the acute lung injury to ischemia-reperfusion injury.


METHODS
The model of ischemia-reperfusion was established. Thirty male Sprague-Dawley rats were randomly divided into control (n = 10), I/R (n = 10), and PS (n = 10) groups.


METHODS
the isolated rat lungs were reperfused for 4 hours. I/R group: the isolated rat lungs were reperfused for 2 hours after 2 hours ischemia. PS group: exogenous pulmonary surfactant (10 mg/100 g BW) were given to the ischemic lungs through bronchus 2 hours before reperfusion. Wet to dry lung weight ratio (W/D) and pulmonary artery pressure (PAP) were checked. Immunohistochemical technique was used to determine the immune reactivity of lung tissues to endothelia nitric oxide synthase (eNOS) and surfactant protein A (SP-A). The pulmonary changes were also observed by light and electronic microscopes.


RESULTS
W/D and PAP in I/R group were significantly higher than in PS group (P < 0.01). The expression of eNOS and SP-A in I/R group were significantly lower than those in PS group (P < 0.05).


CONCLUSIONS
PS can significantly protect lung injury induced by ischemia-reperfusion in the isolated rat lungs. This protective effect is associated with the mechanism that the exogenous PS may reduce SP-A loss in the ischemia-reperfusion and activate the up-regulation of eNOS.
Meng
Ying
Y
Department of Pathology, Cardiovascular Institute and Fu Wai Hospital, CAMS and PUMC, Beijing 100037, China. mengying_zhao@sina.com
Zhao
Ju
J
Ruan
Ying-mao
YM
D000818:Animals; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*
D037662:Pulmonary Surfactant-Associated Protein A; D011663:Pulmonary Surfactants; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat