Mycobacterial phosphatidylinositol mannoside is a natural antigen for CD1d-restricted T cells.
A group of T cells recognizes glycolipids presented by molecules of the CD1 family. The CD1d-restricted natural killer T cells (NKT cells) are primarily considered to be self-reactive. By employing CD1d-binding and T cell assays, the following structural parameters for presentation by CD1d were defined for a number of mycobacterial and mammalian lipids: two acyl chains facilitated binding, and a polar head group was essential for T cell recognition. Of the mycobacterial lipids tested, only a phosphatidylinositol mannoside (PIM) fulfilled the requirements for CD1d binding and NKT cell stimulation. This PIM activated human and murine NKT cells via CD1d, thereby triggering antigen-specific IFN-gamma production and cell-mediated cytotoxicity, and PIM-loaded CD1d tetramers identified a subpopulation of murine and human NKT cells. This phospholipid, therefore, represents a mycobacterial antigen recognized by T cells in the context of CD1d.
Fischer
Karsten
K
Department of Immunology, Max Planck Institute for Infection Biology, Schumannstrasse 21/22, 10117 Berlin, Germany.
Scotet
Emmanuel
E
Niemeyer
Marcus
M
Koebernick
Heidrun
H
Zerrahn
Jens
J
Maillet
Sophie
S
Hurwitz
Robert
R
Kursar
Mischo
M
Bonneville
Marc
M
Kaufmann
Stefan H E
SH
Schaible
Ulrich E
UE
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D018949:Antigens, CD1 / Q000378:metabolism*; D055610:Antigens, CD1d; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets; D051379:Mice; D009161:Mycobacterium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000737:chemistry / Q000276:immunology*; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human; D010716:Phosphatidylinositols; C034154:phosphatidylinositol mannoside; D007371:Interferon-gamma