In vitro mass multiplication of Ophiorrhiza mungo Linn.
A protocol for in vitro mass multiplication of plants through seedling (shoot) cultures was established for Ophiorrhiza mungo. Maximum number of adventitious shoots per shoot culture (10.4 +/- 1.72) was initiated on MS solid medium supplemented with BAP (2.22 microM) after 3 weeks. Shoots were further multiplied (12.8 +/- 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5 +/- 0.94) in MS solid medium containing BAP (0.89 microM). Shoot elongation (1.27 +/- 0.12 cm) was achieved in the medium containing GA3 (1.44 microM) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3 microM) plus NAA (1.07 microM). The plants were successfully established (100%) in the pots containing sand and top soil (1:1) mixture in a period of two weeks.
Jose
Binoy
B
Plant Biotechnology Division, Tropical Botanic Garden & Research Institute Palode, Thiruvananthapuram, 695 562, India.
Satheeshkumar
K
K
D000362:Agar / Q000737:chemistry; D002455:Cell Division; D003470:Culture Media; D066298:In Vitro Techniques; D010937:Plant Growth Regulators / Q000378:metabolism; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology*; D019666:Rubiaceae / Q000378:metabolism*; D012822:Silicon Dioxide / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D010937:Plant Growth Regulators; D012822:Silicon Dioxide; D000362:Agar