title: World-wide survey of an Accord insertion and its association with DDT resistance in Drosophila melanogaster.
abstract: Previous work showed that insecticide resistance in Drosophila melanogaster is correlated with the insertion of an Accord-like element into the 5' region of the cytochrome P450 gene, Cyp6g1. Here, we study the distribution of the Accord-like element in 673 recently collected D. melanogaster lines from 34 world-wide populations. We also examine the extent of microsatellite variability along a 180-kilobase (kb) genomic region of chromosome II encompassing the resistance gene. We confirm a 100% correlation of the Accord insertion with insecticide resistance and a significant reduction in variability extending at least 20 kb downstream of the Cyp6g1 gene. The frequency of the Accord insertion differs significantly between East African (32-55%) and nonAfrican (85-100%) populations. This pattern is consistent with a selective sweep driving the Accord insertion close to fixation in nonAfrican populations as a result of the insecticide resistance phenotype it confers. This study confirms that hitchhiking mapping can be used to identify beneficial mutations in natural populations.
authors.affiliation: Institut für Tierzucht und Genetik, University of Wien, Josef Baumann Gasse 1, 1210 Wien, Austria.
authors.forename: F
authors.initials: F
authors.lastname: Catania
authors.forename: M O
authors.initials: MO
authors.lastname: Kauer
authors.forename: P J
authors.initials: PJ
authors.lastname: Daborn
authors.forename: J L
authors.initials: JL
authors.lastname: Yen
authors.forename: R H
authors.initials: RH
authors.lastname: Ffrench-Constant
authors.forename: C
authors.initials: C
authors.lastname: Schlotterer
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003634:DDT / Q000506:poisoning*; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D012641:Selection, Genetic
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D003577:Cytochrome P-450 Enzyme System; D003634:DDT; C446928:Cyp6g1 protein, Drosophila