title: Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and Archaea in the deep ocean.
abstract: The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4',6'-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%).
authors.affiliation: Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. teira@nioz.nl
authors.forename: Eva
authors.initials: E
authors.lastname: Teira
authors.forename: Thomas
authors.initials: T
authors.lastname: Reinthaler
authors.forename: Annelie
authors.initials: A
authors.lastname: Pernthaler
authors.forename: Jakob
authors.initials: J
authors.lastname: Pernthaler
authors.forename: Gerhard J
authors.initials: GJ
authors.lastname: Herndl
mesh_terms: D001105:Archaea / Q000378:metabolism*; D001345:Autoradiography / Q000379:methods*; D001419:Bacteria / Q000378:metabolism*; D002384:Catalysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012623:Seawater / Q000382:microbiology*