[Preparation of a composite membrane with collagen scaffold material for tissue culture].
OBJECTIVE
To prepare a composite membrane with collagen scaffold material for tissue culture.


METHODS
Pure collagen membrane, collagen/hyaluronic acid (HA) membrane, collagen/chitosan membrane and collagen/chitosan/ HA membrane (abbreviated as composite collagen membrane) were prepared respectively, and their respective biodegradation time and the number of the cells proliferated on them were examined in vitro.


RESULTS
The biodegradation time of pure collagen, collagen/HA and composite collagen membranes in vitro was 1 380, 1 376 and 1 560 min respectively, and the number of cells proliferated on the collagen/HA membrane and the composite collagen membrane were greater than that on the pure collagen membrane.


CONCLUSIONS
Addition of chitosan can decrease the biodegradation of the collagen membrane in vitro, while HA can not; HA Can contribute to cell adhesion and proliferation on the collagen membranes, but chitosan does not.
Huang
Han-ping
HP
Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, China. hhp_xu@yahoo.com.cn
Mou
Shan-song
SS
Ma
An-de
AD
D016475:3T3 Cells; D000818:Animals; D001673:Biodegradation, Environmental; D002455:Cell Division; D002686:Chitin / Q000031:analogs & derivatives*; D048271:Chitosan; D003094:Collagen / Q000737:chemistry*; D046508:Culture Techniques; D006820:Hyaluronic Acid; D008567:Membranes, Artificial; D051379:Mice; D023822:Tissue Engineering
D008567:Membranes, Artificial; D002686:Chitin; D006820:Hyaluronic Acid; D003094:Collagen; D048271:Chitosan