The pattern of c-Fos immunoreactivity in the hindbrain of the rat following stomach distension.
It has been previously shown that the walls of the stomach contain vagal and splanchnic afferents, connected to low and high threshold (LT and HT) gastric receptors, that convey physiological and noxious information to areas of the hindbrain involved mainly in the control of gastrointestinal function. Because distension of the stomach also reflexly increases the sympathetic drive to the cardiovascular system, the present study was planned to examine the pattern of activation of all nuclei encountered throughout the hindbrain in response to gastric distension. In anaesthetized rats, the stimulus was controlled by employing different transmural pressures and frequencies of distension, and c-Fos immunohistochemistry was used to characterize neuronal activation. Low intensity stimulation induced c-Fos expression in the cranial part of nucleus of solitary tract (NTS), the nucleus ambiguus (NA), the lateral reticular area (LRt) and the ventrolateral medulla (RVL/CVL). At low frequency of stimulation c-Fos positive nuclei (p.n.) were found in NTS only. At high frequency of stimulation an increase in c-Fos immunoreactivity was found. High intensity stimulation induced c-Fos expression in area postrema (AP), the lateral vestibular nucleus (LVe) and the caudal part of the NTS. At low frequency, only the number of c-Fos p.n. was increased. Increasing the frequency of stimulation induced c-Fos expression in further nuclei such as the parabrachial nucleus (PBN), the inferior olive subnuclei (IOn), the oral part of spinal trigeminal nucleus (Sp5O) and locus coeruleus (LC). At higher frequencies c-Fos immunoreactivity decreased in NTS and LRt, disappeared in VLM and increased in NA. Thus stomach distension activated several neuronal excitatory and inhibitory circuits that are involved in the control of gastrointestinal function as well as in cardiovascular, respiratory and pain regulation. The differences in c-Fos immunoreactivity induced by changing the distension patterns suggested interactions between groups of vagal and splanchnic afferents.
Sabbatini
M
M
Lab. Anatomia Umana, Dip.to Scienze Mediche, Università del Piemonte Orientale, via Solaroli 17, 28100, Novara, Italy.
Molinari
C
C
Grossini
E
E
Mary
D A S G
DA
Vacca
G
G
Cannas
M
M
D000818:Animals; D031608:Area Postrema / Q000166:cytology / Q000502:physiology; D002320:Cardiovascular Physiological Phenomena; D004558:Electric Stimulation; D007150:Immunohistochemistry; D008297:Male; D010146:Pain / Q000503:physiopathology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012143:Respiratory Physiological Phenomena; D012154:Reticular Formation / Q000166:cytology / Q000502:physiology; D012249:Rhombencephalon / Q000166:cytology / Q000502:physiology*; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology; D013153:Splanchnic Nerves / Q000166:cytology / Q000502:physiology*; D013270:Stomach / Q000294:innervation* / Q000502:physiology / Q000503:physiopathology; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*; D017833:Visceral Afferents / Q000166:cytology / Q000502:physiology*
D016760:Proto-Oncogene Proteins c-fos