HTLV-1 provirus load in peripheral blood lymphocytes of HTLV-1 carriers is diminished by green tea drinking.
Human T-cell lymphotropic virus type 1 (HTLV-1) is causatively associated with adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Since a high level of HTLV-1 provirus load in circulating lymphocytes is thought to be a risk for ATL and HAM/TSP, diminution of HTLV-1 provirus load in the circulation may prevent these intractable diseases. Our previous study (Jpn J Cancer Res 2000; 91: 34-40) demonstrated that green tea polyphenols inhibit in vitro growth of ATL cells, as well as HTLV-1-infected T-cells. The present study aimed to investigate the in vivo effect of green tea polyphenols on HTLV-1 provirus load in peripheral blood lymphocytes on HTLV-1 carriers. We recruited 83 asymptomatic HTLV-1 carriers to examine HTLV-1 provirus DNA with or without administration of capsulated green tea extract powder. Thirty-seven subjects were followed up for 5 months by measuring HTLV-1 provirus load after daily intake of 9 capsules of green tea extract powder per day (equivalent to 10 cups of regular green tea), and 46 subjects lived ad libitum without intake of any green tea capsule. The real-time PCR quantification of HTLV-1 DNA revealed a wide range of variation of HTLV-1 provirus load among asymptomatic HTLV-1 carriers (0.2-200.2 copies of HTLV-1 provirus load per 1000 peripheral blood lymphocytes). Daily intake of the capsulated green tea for 5 months significantly diminished the HTLV-1 provirus load as compared with the controls (P = 0.031). These results suggest that green tea drinking suppresses proliferation of HTLV-1-infected lymphocytes in vivo.
Sonoda
Junichiro
J
Department of Clinical Pharmacology, Faculty of Medicine, Kagoshima University, Kagoshima 890-8520, Japan.
Koriyama
Chihaya
C
Yamamoto
Shinako
S
Kozako
Tomohiro
T
Li
Hong Chuan
HC
Lema
Carolina
C
Yashiki
Shinji
S
Fujiyoshi
Toshinobu
T
Yoshinaga
Mitsuhiro
M
Nagata
Yukihiro
Y
Akiba
Suminori
S
Takezaki
Toshiro
T
Yamada
Katsushi
K
Sonoda
Shunro
S
D000284:Administration, Oral; D000328:Adult; D000368:Aged; D002353:Carrier State; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D015490:HTLV-I Infections / Q000188:drug therapy*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000517:prevention & control / Q000821:virology; D008214:Lymphocytes / Q000502:physiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000517:prevention & control / Q000821:virology; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D011533:Proviruses; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013662:Tea / Q000737:chemistry*; D019562:Viral Load
D004279:DNA, Viral; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea