title: Profiling of sulfoconjugates in urine by using precursor ion and neutral loss scans in tandem mass spectrometry. Application to the investigation of heavy metal toxicity in rats.
abstract: This paper reports a liquid chromatographic/electrospray ionization mass spectrometric (LC/ESI-MS) method for profiling a wide range of structurally different sulfoconjugated compounds in urine and its application to the characterization of biomarkers for heavy metal toxicity in rat urine. Sulfoconjugates were first isolated by solid-phase extraction and the LC separation was performed on a reversed-phase column. Sulfoconjugates were detected in a triple-quadrupole mass spectrometer by simultaneously monitoring constant losses of 80 u (or 80 Th for doubly charged ions), precursors of m/z 80 (SO(3) (-*)) and precursors of m/z 97 (HSO4-). The ESI-MS detection conditions were optimized on dehydroepiandrosterone sulfate and estradiol sulfate and tested on other sulfoconjugates. The analysis of urine samples from humans and rats by using the developed method allowed the detection of about 15 peaks in each mode of detection. It was then applied to the investigation of heavy metal toxicity in rats. Comparative analysis of the chromatographic fingerprints of urine from control and uranium- and cadmium-treated rats showed several variations in the chromatographic pattern of the sulfoconjugates. Diagnostic m/z ratios were confirmed by analyzing individual urine samples and one of the observed variations seemed to be specific to uranium toxicity. The ion responsible for this variation has been identified as 4-ethylphenol sulfate by comparison of its chromatographic retention time and collision-induced dissociation mass spectra (MS(2) and MS(3) performed on a quadrupole ion trap instrument) with those of the synthesized compound.
authors.affiliation: Service de Pharmacologie et d'Immunologie, DSV/DRM, CEA/Saclay, 91191 Gif-sur-Yvette cedex, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lafaye
authors.forename: Christophe
authors.initials: C
authors.lastname: Junot
authors.forename: Béatrice
authors.initials: B
authors.lastname: Ramounet-Le Gall
authors.forename: Paul
authors.initials: P
authors.lastname: Fritsch
authors.forename: Eric
authors.initials: E
authors.lastname: Ezan
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Tabet
mesh_terms: D000818:Animals; D006801:Humans; D007477:Ions / Q000737:chemistry; D019216:Metals, Heavy / Q000633:toxicity / Q000652:urine*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013457:Sulfur Compounds / Q000633:toxicity / Q000652:urine*
chemical_list: D007477:Ions; D019216:Metals, Heavy; D013457:Sulfur Compounds