title: Serum concentrations of MCP-1 and RANTES in patients during aortic surgery: the relationship with ischemia-reperfusion.
abstract: BACKGROUND
  Surgical trauma is associated with depression of the immune system, which results in a high complication rate following abdominal aortic aneurysm (AAA) repair. Monocyte chemotactic protein-1 (MCP-1) and regulated-on-activation normal T cell expressed and secreted (RANTES) protein are important mediators of the immune and inflammatory response. The aim of this study was to determine whether there is any relationship between MCP-1 or RANTES and operative injury and ischemia-reperfusion during AAA surgery in human.
  
  
  METHODS
  Peripheral blood samples were taken from 12 patients before surgery, after anesthesia induction, before unclamping of aorta (PreXoff), 90 min after unclamping (90 minXoff), and at 24 and 48 h after surgery.
  
  
  RESULTS
  The MCP-1 and RANTES serum concentrations were measured with the ELISA technique. MCP-1 concentration significantly increased after reperfusion (90 minXoff) in comparison with the PreXoff level (p=0.001). Twenty-four hours after AAA repair, MCP-1 significantly decreased 269-225 pg/ml (p=0.005) and reached preoperative value. RANTES level was higher in AAA patients before surgery than in controls (p=0.025) and decreased significantly after ischemia-reperfusion to 13 ng/ml (p< 0.001) at 90 minXoff. We showed increases in RANTES concentration to 26 ng/ml on the 1st and to 31 ng/ml on the 2nd day after surgery (p=0.020, p=0.012, respectively) compared with the 90 min Xoff level.
  
  
  CONCLUSIONS
  Ischemia-reperfusion during AAA repair results in an increase in MCP-1 and decrease in RANTES concentrations in serum. The changes in chemokine concentrations may influence the development of immunosuppression after AAA repair, contributing to the postoperative course.
authors.affiliation: Department of Anesthesiology and Intensive Therapy, Medical University, Białystok, Poland. jedynaka@amb.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Jedynak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Siemiatkowski
authors.forename: Marek
authors.initials: M
authors.lastname: Gacko
authors.forename: Barbara
authors.initials: B
authors.lastname: Mroczko
authors.forename: Jacek
authors.initials: J
authors.lastname: Borkowski
mesh_terms: D000328:Adult; D000368:Aged; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery*; D018932:Chemokine CCL2 / Q000097:blood* / Q000276:immunology; D018946:Chemokine CCL5 / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000276:immunology*; D013223:Statistics as Topic
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5