Requirement of matrix metalloproteinase-9 for the transformation of human mammary epithelial cells by Stat3-C.
Persistently activated Stat3 is found in many different cancers, including approximately 60% of breast tumors. Here, we demonstrate that a constitutively activated Stat3 transforms immortalized human mammary epithelial cells and that this oncogenic event requires the activity of matrix metalloproteinase-9 (MMP-9). By immunohistochemical analysis, we observe a positive correlation between strong MMP-9 expression and tyrosine phosphorylated Stat3 in primary breast cancer specimens. These results demonstrate a relationship between activated Stat3 and MMP-9 in breast oncogenesis.
Dechow
Tobias N
TN
Laboratory of Molecular Cell Biology, The Rockefeller University, New York, NY 10021, USA.
Pedranzini
Laura
L
Leitch
Andrea
A
Leslie
Kenneth
K
Gerald
William L
WL
Linkov
Irina
I
Bromberg
Jacqueline F
JF
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism*
D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D020780:Matrix Metalloproteinase 9