Determination of bisphosphonates by ion chromatography-inductively coupled plasma mass spectrometry.
An ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) was introduced in the analysis of bisphosphonates. Two compounds (alendronic acid and etidronic acid) were separated on a Dionex AS-7 anion-exchange column with dilute nitric acid employed as the mobile phase. The analytes were detected at m/z 31, as they contain phosphorus. The detection limits achieved were 0.20 mg l(-1) for alendronic acid and 0.05 mg l(-1) for etidronic acid. Since the determination of phosphorus by ICP-MS is difficult due to polyatomic interferences at m/z 31 (15N16O+, 14N16O1H+, and 12C1H(3)16O+), a detailed study of the influence of plasma parameters on phosphorus and background signal was performed.
Kovacevic
Miroslav
M
National Institute of Chemistry, PO Box 660, SI-1001 Ljubljana, Slovenia.
Gartner
Andrej
A
Novic
Milko
M
D002853:Chromatography, Liquid / Q000379:methods*; D004164:Diphosphonates / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity
D004164:Diphosphonates