title: Prognostic and biologic significance of chromosomal imbalances assessed by comparative genomic hybridization in multiple myeloma.
abstract: Cytogenetic abnormalities, evaluated either by karyotype or by fluorescence in situ hybridization (FISH), are considered the most important prognostic factor in multiple myeloma (MM). However, there is no information about the prognostic impact of genomic changes detected by comparative genomic hybridization (CGH). We have analyzed the frequency and prognostic impact of genetic changes as detected by CGH and evaluated the relationship between these chromosomal imbalances and IGH translocation, analyzed by FISH, in 74 patients with newly diagnosed MM. Genomic changes were identified in 51 (69%) of the 74 MM patients. The most recurrent abnormalities among the cases with genomic changes were gains on chromosome regions 1q (45%), 5q (24%), 9q (24%), 11q (22%), 15q (22%), 3q (16%), and 7q (14%), while losses mainly involved chromosomes 13 (39%), 16q (18%), 6q (10%), and 8p (10%). Remarkably, the 6 patients with gains on 11q had IGH translocations. Multivariate analysis selected chromosomal losses, 11q gains, age, and type of treatment (conventional chemotherapy vs autologous transplantation) as independent parameters for predicting survival. Genomic losses retained the prognostic value irrespective of treatment approach. According to these results, losses of chromosomal material evaluated by CGH represent a powerful prognostic factor in MM patients.
authors.affiliation: Servicio de Hematología, Hospital Universitario de Salamanca, Paseo San Vicente 58-182, 37007 Salamanca, Spain.
authors.forename: Norma C
authors.initials: NC
authors.lastname: Gutiérrez
authors.forename: Juan L
authors.initials: JL
authors.lastname: García
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Hernández
authors.forename: Eva
authors.initials: E
authors.lastname: Lumbreras
authors.forename: Mariana
authors.initials: M
authors.lastname: Castellanos
authors.forename: Ana
authors.initials: A
authors.lastname: Rasillo
authors.forename: Gema
authors.initials: G
authors.lastname: Mateo
authors.forename: José M
authors.initials: JM
authors.lastname: Hernández
authors.forename: Sonia
authors.initials: S
authors.lastname: Pérez
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Jesús F
authors.initials: JF
authors.lastname: San Miguel
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000401:mortality; D015999:Multivariate Analysis; D009693:Nucleic Acid Hybridization; D011379:Prognosis; D016019:Survival Analysis; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains