title: Enzymolysis approach to compare cu availability from human milk and infant formulas.
abstract: The purpose of the present paper is to develop an easy and quick in vitro method to compare copper availability from breast milk and infant formulas. This study focuses on the differences caused by the use of pH 2.0 (adult gastric pH) or pH 5.0 (newborn gastric pH) in the first stage of the enzymolysis. pH affects Cu solubility, a possible estimator of the availability. Selection of a digestor, times of enzymolysis, centrifugation parameters, and Cu determination by ETAAS were discussed as well. Percentage of Cu solubility was larger from breast milk (gastric pH 2.0, 65.3 +/- 14.0 vs 40.0 +/- 13.9%; gastric pH 5.0, 61.2 +/- 16.5 vs 26.6 +/- 10.3%), but the soluble content was larger from infant formulas for both pHs (gastric pH 2.0, 245.3 +/- 82.1 vs 113.0 +/- 103.4 ng mL(-1); gastric pH 2.0, 169.3 +/- 76.9 vs 75.3 +/- 21.9 vs ng mL(-1)).
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avda de las Ciencias, E-15782 Santiago de Compostela, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Peña
authors.forename: R
authors.initials: R
authors.lastname: Domínguez
authors.forename: A
authors.initials: A
authors.lastname: Bermejo
authors.forename: J A
authors.initials: JA
authors.lastname: Cocho
authors.forename: J M
authors.initials: JM
authors.lastname: Fraga
authors.forename: P
authors.initials: P
authors.lastname: Bermejo
mesh_terms: D001682:Biological Availability; D002498:Centrifugation; D003300:Copper / Q000032:analysis / Q000493:pharmacokinetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D041943:Infant Formula; D008895:Milk, Human; D012995:Solubility; D014461:Ultracentrifugation
chemical_list: D003300:Copper