title: Moderate daily intake of red wine inhibits mural thrombosis and monocyte tissue factor expression in an experimental porcine model.
abstract: BACKGROUND
  Moderate consumption of red wine has been epidemiologically associated with a reduction in cardiovascular disease, but its mechanism of action is not fully understood. The objective was to study whether the protective effects of a daily intake of red wine (Tempranillo, 12.8% alcohol vol/vol) could be related to inhibition of thrombosis in an experimental model of diet-induced hyperlipemia.
  
  
  RESULTS
  For 100 days, animals were fed a western-type proatherogenic diet containing 2% cholesterol and 20% saturated fat. Three doses of red wine were studied (20, 30, and 40 g wine-ethanol/d) and compared with placebo-control animals not taking any wine. Thrombosis under flow conditions was evaluated by radioisotopic quantification of deposited platelets on damaged arteries. Changes in RhoA translocation in platelets and monocyte tissue factor expression were also analyzed. Mural platelet deposition was significantly reduced in animals ingesting red wine with their food. Expression of RhoA in the platelet cytoplasm (inactive form) was increased in wine-fed animals. Tissue factor mRNA expression in lipopolysaccharide-stimulated monocytes was reduced in wine-fed animals. Total cholesterol levels were not significantly different among groups.
  
  
  CONCLUSIONS
  Moderate red wine intake significantly reduces platelet deposition triggered by damaged vessel wall, partially explained by inhibition of RhoA translocation to the platelet membrane. Hence, a daily moderate intake of wine seems to inhibit different pathways that converge in a reduced thrombotic risk on vessel wall injury.
authors.affiliation: Cardiovascular Research Center, Consejo Superior de Investigaciones Científicas-Institut Català de Ciències Cardiovasculars, Hospital Santa Creu i Sant Pau, UAB, Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Casani
authors.forename: E
authors.initials: E
authors.lastname: Segales
authors.forename: G
authors.initials: G
authors.lastname: Vilahur
authors.forename: A
authors.initials: A
authors.lastname: Bayes de Luna
authors.forename: L
authors.initials: L
authors.lastname: Badimon
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D004036:Diet, Atherogenic; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D006331:Heart Diseases / Q000517:prevention & control*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D010974:Platelet Aggregation; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D034421:Sus scrofa; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D013927:Thrombosis / Q000517:prevention & control*; D014920:Wine; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D013925:Thromboplastin; D002784:Cholesterol; D020742:rhoA GTP-Binding Protein