title: Nosocomial outbreak of respiratory syncytial virus subgroup B variants with the 60 nucleotides-duplicated G protein gene.
abstract: In January 2001, 20 children among 40 residents under 2 years old at a nursery home in Sapporo, Japan had respiratory symptoms and were confirmed as having respiratory syncytial virus (RSV) infection by a conventional diagnostic kit. Nasopharyngeal aspirates were collected from four RSV-positive patients and total RNA was extracted directly from the specimens for the analysis of RSV grouping and genotyping. All four RSV strains had the same G protein gene sequence of subgroup B and were assigned to identical strains. Interestingly, the G protein gene had a duplication of 60 nucleotides at the C-terminal third of the G protein gene in which three nucleotides differed each other. The predicted polypeptide is lengthened by 20 amino acids. The clinical picture of these cases was not different from those of patients with other RSV strains. These novel mutations were thought to be introduced in vivo.
authors.affiliation: Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan. nkazu@sapmed.ac.jp
authors.forename: Kazushige
authors.initials: K
authors.lastname: Nagai
authors.forename: Hotaka
authors.initials: H
authors.lastname: Kamasaki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kuroiwa
authors.forename: Lisa
authors.initials: L
authors.lastname: Okita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003428:Cross Infection / Q000453:epidemiology / Q000821:virology*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004196:Disease Outbreaks; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D007564:Japan; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D009724:Nurseries, Infant; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins