Heat-induced changes in the ultrasonic properties of whey proteins.
The physical aggregation of commercial whey protein isolate (WPI) and purified beta-lactoglobulin was studied by ultrasound spectroscopy. Protein samples were dialyzed to achieve constant ionic strength backgrounds of 0.01 and 0.1 NaCl, and gelation was induced in situ at constant temperatures (from 50 to 75 degrees C) or with a temperature ramp from 20 to 85 degrees C. Changes in the ultrasonic properties were shown in the early stages of heating, at temperatures below those reported for protein denaturation. During heating, the relative ultrasound velocity (defined as the difference between sample velocity and reference velocity) decreased continuously with temperature, indicating a rearrangement of the hydration layer of the protein and an increase in compressibility of the protein shell. At temperatures <50 degrees C the ultrasonic attenuation decreased, and <65 degrees C both velocity and attenuation differentials showed increasing values. A sharp decrease in the relative velocity and an increase in the attenuation at 70 degrees C were indications of "classical" protein denaturation and the formation of a gel network. Values of attenuation were significantly different between samples prepared with 0.01 and 0.1 M NaCl, although no difference was shown in the overall ultrasonic behavior. WPI and beta-lactoglobulin showed similar ultrasonic properties during heating, but some differences were noted in the values of attenuation of WPI solutions, which may relate to a less homogeneous distribution of aggregates caused by the presence of alpha-lactalbumin and other minor proteins in WPI.
Corredig
Milena
M
Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. mcorredi@uoguelph.ca
Verespej
Edita
E
Dalgleish
Douglas G
DG
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005782:Gels; D006358:Hot Temperature; D007782:Lactoglobulins / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D009994:Osmolar Concentration; D011489:Protein Denaturation; D013057:Spectrum Analysis; D013696:Temperature; D014465:Ultrasonics; D000067816:Whey Proteins
D005782:Gels; D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins