title: Maternal and environmental factors in early cloned embryo development.
abstract: Cloning by somatic cell nuclear transfer (SCNT) in mammals has revealed the remarkable ability of an oocyte to reprogram somatic cell nuclei and induce them to recapitulate the developmental program. Despite the success, cloning remains very inefficient. This review summarizes recent observations from cloning in mice that reveal some of the likely causes for the present inefficiency. One cause appears to be the slow pace of reprogramming combined with the early onset of genome transcription, which together cause cloned embryos to elaborate many somatic cell characteristics even before the first cleavage division. The altered phenotypes of cloned embryos render standard embryo culture conditions grossly sub-optimum. Another cause appears to be a hitherto unappreciated contribution of spindle-associated factors to early embryo development. As current procedures remove the spindle and associated factors, cloned embryos lack these factors. These observations are providing new insight into basic mammalian embryology. They also reveal possible changes to protocols that could improve the overall success of cloning.
authors.affiliation: The Fels Institute for Cancer Research and Molecular Biology and Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: S
authors.initials: S
authors.lastname: Gao
authors.forename: K E
authors.initials: KE
authors.lastname: Latham
mesh_terms: D000818:Animals; D002875:Chromosomes; D019976:Cloning, Organism / Q000379:methods; D003470:Culture Media; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D053652:Nuclear Transfer Techniques; D010053:Ovary / Q000166:cytology; D010641:Phenotype; D013573:Synaptonemal Complex / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media