title: Distinct effects of SP-B and SP-C on the uptake of surfactant-like liposomes by alveolar cells in vivo and in vitro.
abstract: The effects of surfactant protein B (SP-B) and SP-C on the uptake of surfactant-like liposomes by alveolar type II cells and alveolar macrophages were studied both in vivo and in vitro. In vivo, mechanically ventilated rats were intratracheally instilled with fluorescently labeled liposomes that had SP-B and/or SP-C incorporated in different concentrations. Consequently, the alveolar cells were isolated, and cell-associated fluorescence was determined using flow cytometry. The results show that the incorporation of SP-B does not influence the uptake, and it also does not in the presence of essential cofactors. The inclusion of SP-C in the liposomes enhanced the alveolar type II cells at a SP-C to lipid ratio of 2:100. If divalent cations (calcium and magnesium) were present at physiological concentrations in the liposome suspension, uptake of liposomes by alveolar macrophages was also enhanced. In vitro, the incorporation of SP-B affected uptake only at a protein-to-lipid ratio of 8:100, whereas the inclusion of SP-C in the liposomes leads to an increased uptake at a protein-to-lipid ratio of 1:100. From these results, it can be concluded that SP-B is unlikely to affect uptake of surfactant, whereas SP-C in combination with divalent cations and other solutes are capable of increasing the uptake.
authors.affiliation: Department of Anesthesiology, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: D L H
authors.initials: DL
authors.lastname: Poelma
authors.forename: L J
authors.initials: LJ
authors.lastname: Zimmermann
authors.forename: W A
authors.initials: WA
authors.lastname: van Cappellen
authors.forename: J J
authors.initials: JJ
authors.lastname: Haitsma
authors.forename: B
authors.initials: B
authors.lastname: Lachmann
authors.forename: J F
authors.initials: JF
authors.lastname: van Iwaarden
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005453:Fluorescence; D066298:In Vitro Techniques; D008081:Liposomes / Q000493:pharmacokinetics*; D016676:Macrophages, Alveolar / Q000166:cytology; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism*; D011659:Pulmonary Gas Exchange / Q000502:physiology; D037701:Pulmonary Surfactant-Associated Protein B / Q000494:pharmacology*; D037721:Pulmonary Surfactant-Associated Protein C / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
chemical_list: D008081:Liposomes; D037701:Pulmonary Surfactant-Associated Protein B; D037721:Pulmonary Surfactant-Associated Protein C