title: Cytotoxic effects of mansonone E and F isolated from Ulmus pumila.
abstract: Two sesquiterpenoids, mansonone E (ME) and mansonone F (MF) were first isolated from the dried root bark of Ulmus pumila (shironire in Japanese), and their antiproliferative activities on human tumor cells were evaluated in vitro. ME had more potent cytotoxic effects on four tumor cell lines, human cervical cancer HeLa, human malignant melanoma A375-S2, human breast cancer MCF-7, and human histiocytic lymphoma U937, than those of MF. The results showed that ME induced oligonucleosomal fragmentation of DNA in HeLa cells and activated caspase-3, followed by the degradation of the inhibitor of caspase-activated DNase, decreased the expression of anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-(XL), and increased that of proapoptotic Bax.
authors.affiliation: Department of Pharmacognosy, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: MingYu
authors.initials: M
authors.lastname: Xia
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tashiro
authors.forename: Satoshi
authors.initials: S
authors.lastname: Onodera
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000633:toxicity*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D018517:Plant Roots; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000633:toxicity*; D034403:Ulmus
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006131:Growth Inhibitors; D009285:Naphthoquinones; D010936:Plant Extracts; D012717:Sesquiterpenes; C496164:mansonone E; C496018:mansonone F