In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris.
The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
Cha
Hyung Joon
HJ
Department of Chemical Engineering and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea. hjcha@postech.ac.kr
Dalal
Nimish G
NG
Bentley
William E
WE
D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D010843:Pichia / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors
D007376:Interleukin-2; D049452:Green Fluorescent Proteins