title: Production of transgenic chimeric rabbits and transmission of the transgene through the germline.
abstract: Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras.
authors.affiliation: Department of Animal Biology, Agricultural Biotechnology Center, Gödöllö, Hungary.
authors.forename: Szilárd
authors.initials: S
authors.lastname: Bodó
authors.forename: Elen
authors.initials: E
authors.lastname: Gócza
authors.forename: Tamás
authors.initials: T
authors.lastname: Révay
authors.forename: László
authors.initials: L
authors.lastname: Hiripi
authors.forename: Bogdan
authors.initials: B
authors.lastname: Carstea
authors.forename: András
authors.initials: A
authors.lastname: Kovács
authors.forename: Lilla
authors.initials: L
authors.lastname: Bodrogi
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Bösze
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D046528:Chimerism; D002874:Chromosome Mapping; D017931:DNA Primers; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D018095:Germ-Line Mutation; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011817:Rabbits; D013480:Superovulation
chemical_list: D017931:DNA Primers; C078147:F8 protein, human; D005169:Factor VIII