Overexpression of the Wilms' tumor gene WT1 in pancreatic ductal adenocarcinoma.
The expression of the Wilms' tumor gene WT1 was examined by immunohistochemistry in 40 cases of pancreatic ductal adenocarcinoma. WT1 protein was expressed in 30 (75%) of the 40 pancreatic ductal adenocarcinomas, but not in the remaining 10 (25%). In normal pancreatic ductal cells, WT1 protein was undetectable. No correlations between WT1 expression and clinicopathological parameters such as age, sex, T or N stage, tumor location, and tumor differentiation were observed. Treatment with WT1 antisense oligomers significantly inhibited the growth of five human pancreatic cancer cell lines, PSN1, MiaPaCa2, ASPC1, BxPC3, and PCI6, expressing the WT1 gene. These results indicate an important role of the WT1 gene in the tumorigenesis of pancreatic ductal adenocarcinoma expressing WT1 and provide a rationale for new treatment strategies to treat pancreatic ductal adenocarcinoma by targeting the WT1 gene and its product.
Oji
Yusuke
Y
Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Nakamori
Shoji
S
Fujikawa
Masahiro
M
Nakatsuka
Shin-Ichi
S
Yokota
Asumi
A
Tatsumi
Naoya
N
Abeno
Sakie
S
Ikeba
Ai
A
Takashima
Satoshi
S
Tsujie
Masanori
M
Yamamoto
Hirofumi
H
Sakon
Masato
M
Nezu
Riichiro
R
Kawano
Kiyoshi
K
Nishida
Sumiyuki
S
Ikegame
Kazuhiro
K
Kawakami
Manabu
M
Tsuboi
Akihiro
A
Oka
Yoshihiro
Y
Yoshikawa
Kiyoshi
K
Aozasa
Katsuyuki
K
Monden
Morito
M
Sugiyama
Haruo
H
D000230:Adenocarcinoma / Q000235:genetics* / Q000503:physiopathology; D000368:Aged; D000369:Aged, 80 and over; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000503:physiopathology; D002471:Cell Transformation, Neoplastic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016376:Oligonucleotides, Antisense; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000503:physiopathology; D015854:Up-Regulation; D025721:WT1 Proteins / Q000096:biosynthesis*
D016376:Oligonucleotides, Antisense; D025721:WT1 Proteins