Elevated urinary 8-isoprostaglandin F(2alpha) in females with Graves' hyperthyroidism.
In this study, we investigate how oxidative stress alters the urinary F2-isoprostanes' level and we examine the correlation between 8-isoPGF(2alpha) and thyroid hormones in female patients with Graves' hyperthyroidism. We quantitatively determined the concentrations of urinary F2-isoprostanes using gas chromatography-mass spectrometry in the selected ion-monitoring mode. We recruited individuals in the following three groups of subjects for this study: (1) female hyperthyroidism patients (n = 14, 21-71 years), (2) female hypothyroidism patients (n = 16, 16-66 years), and (3) female age-matched normal controls (n = 10, 20-61 years). The average concentration of 8-iso prostaglandin F(2alpha) (8-isoPGF(2alpha)) in hyperthyroidism patients was significantly higher than that in the healthy controls (P < 0.05). The concentration of 8-isoPGF(2alpha) in hypothyroidism patients was similar to that in normal controls. Although the level of 8-isoPGF(2alpha) in two of the patients was slightly elevated, the P value was not significant (NS). Our data suggest that the increased level of urinary 8-isoPGF(2alpha) may reflect possible oxidation injuries in hyperthyroidism patients due to the high level of thyroid hormones. Therefore, the elevated 8-isoPGF(2alpha) in patients could be used as an important biomarker for hyperthyroidism disease.
Kim
Kyung Mee
KM
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, South Korea.
Paeng
Ki-Jung
KJ
Hahm
Jong Ryeal
JR
Kim
Deok Ryong
DR
Chung
Bong Chul
BC
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006111:Graves Disease / Q000652:urine*; D006801:Humans; D007037:Hypothyroidism / Q000652:urine; D008875:Middle Aged; D011237:Predictive Value of Tests; D013963:Thyroid Hormones / Q000378:metabolism
D015415:Biomarkers; D013963:Thyroid Hormones; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost