Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens.
Real-time PCR, using dual-labeled fluorescent probes targeting the beta-giardin gene, was used to detect Giardia lamblia in human stool specimens and to discriminate between isolates from the two major genetic assemblages of G. lamblia infective to humans, assemblages A and B.
Guy
Rebecca A
RA
Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Rd. N, Mississauga, Ontario, Canada L5L 1C6. rguy@utm.utoronto.ca
Xiao
Chongxie
C
Horgen
Paul A
PA
D000818:Animals; D001483:Base Sequence; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005243:Feces / Q000469:parasitology*; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia