Induction of apoptosis by ar-turmerone on various cell lines.
This study investigated the cytotoxic effect of ar-turmerone isolated from turmeric (Curcuma longa L) on the K562, L1210, U937 and RBL-2H3 cell lines by the MTT assay. Ar-turmerone exhibited potent cytotoxicity on these cancer cell lines. The IC50 values of ar-turmerone on these cell lines were 20-50 microg/ml. They showed an increased inhibition ratio on cell viability according to the drug concentration < or = 80 microg/ml in the cell lines tested. The DNA fragmentation that is a characteristic of apoptosis due to ar-turmerone occurred in a concentration- and time-dependent manner in the K562, L1210, U937 and RBL-2H3 cancer cell lines. These results suggest that ar-turmerone can induce the apoptotic activity in the K562, L1210, U937 and RBL-2H3 cells.
Ji
Mingjie
M
School of Life Science, Shandong University, Jinan 250100, China.
Choi
Jaesue
J
Lee
Jeongnyeo
J
Lee
Yongkyu
Y
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D004396:Coloring Agents / Q000494:pharmacology; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D007659:Ketones / Q000302:isolation & purification / Q000494:pharmacology*; D008956:Models, Chemical; D010936:Plant Extracts / Q000378:metabolism*; D051381:Rats; D012717:Sesquiterpenes; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014050:Toluene / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D020298:U937 Cells
D004396:Coloring Agents; D007659:Ketones; D010936:Plant Extracts; D012717:Sesquiterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C078098:ar-turmerone; D014050:Toluene; C022616:thiazolyl blue