title: Observation of electrostatically released DNA from gold electrodes with controlled threshold voltages.
abstract: DNA oligo-nucleotides, localized at Au metal electrodes in aqueous solution, are found to be released when applying a negative bias voltage to the electrode. The release was confirmed by monitoring the intensity of the fluorescence of cyanine dyes (Cy3) linked to the 5' end of the DNA. The threshold voltage of the release changes depending on the kind of linker added to the DNA 3'-terminal. The amount of released DNA depends on the duration of the voltage pulse. Using this technique, we can retain DNA at Au electrodes or Au needles, and release the desired amount of DNA at a precise location in a target. The results suggest that DNA injection into living cells is possible with this method.
authors.affiliation: Fujitsu Laboratories Ltd., 10-1 Morinosato-Wakamiya, Atsugi 243-0197, Japan.
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Takeishi
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rant
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Karin
authors.initials: K
authors.lastname: Buchholz
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Usuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Arinaga
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takemoto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Marc
authors.initials: M
authors.lastname: Tornow
authors.forename: Shozo
authors.initials: S
authors.lastname: Fujita
authors.forename: Gerhard
authors.initials: G
authors.lastname: Abstreiter
authors.forename: Naoki
authors.initials: N
authors.lastname: Yokoyama
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055672:Static Electricity; D013997:Time Factors
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D006046:Gold; D004247:DNA