title: The binding sites for the chromatin insulator protein CTCF map to DNA methylation-free domains genome-wide.
abstract: All known vertebrate chromatin insulators interact with the highly conserved, multivalent 11-zinc finger nuclear factor CTCF to demarcate expression domains by blocking enhancer or silencer signals in a position-dependent manner. Recent observations document that the properties of CTCF include reading and propagating the epigenetic state of the differentially methylated H19 imprinting control region. To assess whether these findings may reflect a universal role for CTCF targets, we identified more than 200 new CTCF target sites by generating DNA microarrays of clones derived from chromatin-immunopurified (ChIP) DNA followed by ChIP-on-chip hybridization analysis. Target sites include not only known loci involved in multiple cellular functions, such as metabolism, neurogenesis, growth, apoptosis, and signalling, but potentially also heterochromatic sequences. Using a novel insulator trapping assay, we also show that the majority of these targets manifest insulator functions with a continuous distribution of stringency. As these targets are generally DNA methylation-free as determined by antibodies against 5-methylcytidine and a methyl-binding protein (MBD2), a CTCF-based network correlates with genome-wide epigenetic states.
authors.affiliation: Department of Development & Genetics, Evolution Biology Centre, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.
authors.forename: Rituparna
authors.initials: R
authors.lastname: Mukhopadhyay
authors.forename: WenQiang
authors.initials: W
authors.lastname: Yu
authors.forename: Joanne
authors.initials: J
authors.lastname: Whitehead
authors.forename: JunWang
authors.initials: J
authors.lastname: Xu
authors.forename: Magda
authors.initials: M
authors.lastname: Lezcano
authors.forename: Svetlana
authors.initials: S
authors.lastname: Pack
authors.forename: Chandrasekhar
authors.initials: C
authors.lastname: Kanduri
authors.forename: Meena
authors.initials: M
authors.lastname: Kanduri
authors.forename: Vasudeva
authors.initials: V
authors.lastname: Ginjala
authors.forename: Alexander
authors.initials: A
authors.lastname: Vostrov
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Quitschke
authors.forename: Igor
authors.initials: I
authors.lastname: Chernukhin
authors.forename: Elena
authors.initials: E
authors.lastname: Klenova
authors.forename: Victor
authors.initials: V
authors.lastname: Lobanenkov
authors.forename: Rolf
authors.initials: R
authors.lastname: Ohlsson
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D002874:Chromosome Mapping; D018899:CpG Islands; D019175:DNA Methylation; D015342:DNA Probes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D006824:Hybridization, Genetic; D008099:Liver / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics*
chemical_list: D000076246:CCCTC-Binding Factor; C000619698:Ctcf protein, mouse; D015342:DNA Probes; D004268:DNA-Binding Proteins; D012097:Repressor Proteins