[Protective effect of specific antibody in serum of convalescent patient with SARS].
OBJECTIVE
To investigate inhibitory effect of serum severe acute respiratory syndrome (SARS) -specific antibodies from convalescent patients after half an year of onset on SARS-CoV-mediated cytopathic response.


METHODS
SARS-CoV immunoglobulin G (IgG) antibody was determined by enzyme linked immunoadsorbent assay (ELISA). Twelve serum samples from convalescent patients, diluted by 1:8 with maintenance medium, were mixed with the three dilution supernatants of SARS-CoV. SARS-CoV were isolated, cultured and identified by the Guangzhou Institute of Respiratory Disease, and cultured with Vero E6 cell suspension. The extent of cytopathic response was observed.


RESULTS
The absorbance (A) value of SARS-CoV IgG antibody ranged from 0.81 to 2.06 in patients after half an year of SARS onset, and form 0.79 to 2.01 in patients before half an year of SARS onset. The extent of cytopathic response was decreased by more than 25% in all 12 convalescent patients, as compared with control serum.


CONCLUSIONS
The A value of SARS-CoV IgG antibody in serum of convalescent patients tended to elevate in half an year after SARS onset. SARS-CoV IgG antibody could inhibit SARS-CoV-mediated cytopathic response, indicating it might be one of protective antibodies.
Li
Yong
Y
Guangzhou Institute of Respiratory Disease, Guangzhou 510120, Guangdong, China. liyong999@163.net
Xu
Jun
J
Mo
Hong-ying
HY
Lai
Ke-fang
KF
Tan
Ya-xia
YX
Pi
Xiang-ying
XY
Ren
Xiao-Lan
XL
Zeng
Guang-qiao
GQ
Liu
Miao-Fang
MF
Zhong
Nan-shan
NS
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G