Enzyme-catalysed synthesis of galactosylated 1D- and 1L-chiro-inositol, 1D-pinitol, myo-inositol and selected derivatives using the beta-galactosidase from the thermophile Thermoanaerobacter sp. strain TP6-B1.
The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.
Hart
Joanne B
JB
Carbohydrate Chemistry Team, Industrial Research Ltd, PO Box 31-310, Lower Hutt, New Zealand. j.hart@irl.cri.nz
Kröger
Lars
L
Falshaw
Andrew
A
Falshaw
Ruth
R
Farkas
Erzsébet
E
Thiem
Joachim
J
Win
Anna L
AL
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry*; D006031:Glycosylation; D007294:Inositol / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D008745:Methylation; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D046968:Thermoanaerobacter / Q000201:enzymology*; D001616:beta-Galactosidase / Q000737:chemistry* / Q000302:isolation & purification
C021730:pinitol; D007294:Inositol; D001616:beta-Galactosidase; D005690:Galactose