Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection.
This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD, a column packed with titanium dioxide resin was used. The titanium dioxide column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve.
Sekiguchi
Yoko
Y
Department of Biological Sciences, Faculty of Science, Nara Women's University, Kitauoyanishimachi, Nara City, Nara 630-8506, Japan. sekiguchi@dionex.co.jp
Mitsuhashi
Naoto
N
Inoue
Yoshinori
Y
Yagisawa
Hitoshi
H
Mimura
Tetsuro
T
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004563:Electrochemistry / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013403:Sugar Phosphates / Q000032:analysis*; D014025:Titanium / Q000737:chemistry*
D013403:Sugar Phosphates; C009495:titanium dioxide; D014025:Titanium