title: The expression of Sprouty1, an inhibitor of fibroblast growth factor signal transduction, is decreased in human prostate cancer.
abstract: A considerable body of evidence indicates that alterations of fibroblast growth factors (FGFs) and their receptors contribute to prostate cancer progression. Recently, a new family of regulators of FGF activity has been identified. The Sprouty gene family negatively regulates FGF signaling in a variety of systems and could potentially limit the biological activity of FGFs in prostate cancer. Immunohistochemical analysis of normal and neoplastic prostate tissues using tissue microarrays revealed that Sprouty1 protein is down-regulated in approximately 40% of prostate cancers when compared with matched normal prostate. By quantitative real-time PCR analysis, we found that Sprouty1 mRNA levels were significantly decreased in prostate cancers in vivo in comparison with normal prostate. In prostate cancer cell lines, there is loss of the normal up-regulation of Sprouty1 mRNA and protein in response to FGFs. The decrease in Sprouty1 expression in the human prostate cancer, despite elevated levels of FGF ligands and FGF receptors, implies a loss of an important growth regulatory mechanism in prostate cancers that may potentiate the effects of increased FGF and FGF receptor expression in prostate cancer.
authors.affiliation: Department of Pathology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Bernard
authors.initials: B
authors.lastname: Kwabi-Addo
authors.forename: Jianghua
authors.initials: J
authors.lastname: Wang
authors.forename: Halime
authors.initials: H
authors.lastname: Erdem
authors.forename: Ajula
authors.initials: A
authors.lastname: Vaid
authors.forename: Patricia
authors.initials: P
authors.lastname: Castro
authors.forename: Gustavo
authors.initials: G
authors.lastname: Ayala
authors.forename: Michael
authors.initials: M
authors.lastname: Ittmann
mesh_terms: D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; C486055:SPRY1 protein, human; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors