title: Influence of beta-amyloid fibrils on the interactions between red blood cells and endothelial cells.
abstract: Alzheimer's disease is associated with vascular amyloidosis. As blood flows through the microcirculation, red blood cells (RBCs) come in contact with the vasculature. RBCs as well as endothelial cells (ECs) are known to bind beta amyloid fibrils. This suggests that a potential effect of amyloidosis may involve the interactions of RBCs with ECs lining the wall of the blood vessels mediated by amyloid fibrils. We have studied the effect of beta-amyloid peptide[1-40] (Abeta1-40) fibrils on the interactions of murine RBCs with ECs derived from bovine lung microvascular endothelium (BLMVEC) as well as bovine pulmonary arterial endothelium (BPAEC) in culture. We show that the initial incorporation of Abeta fibrils onto either RBCs or ECs cause RBCs to adhere to the ECs with greater affinity for the microvascular cells than the arterial cells. In addition, there is a transfer of Abeta fibrils between the RBCs and the ECs. Both the transfer and adhesion occurs when the amyloid fibrils are on the ECs or on the RBCs. However, with the amyloid fibrils on the RBCs, the adhesion and the transfer are greater than with the fibrils on the ECs. These results suggest that amyloidosis may affect the flow of RBCs through the microcirculation and that RBCs may play a role in propagating amyloidosis through the vasculature.
authors.affiliation: Molecular Dynamics Section, National Institute on Aging, Baltimore, MD, USA.
authors.forename: Luke B
authors.initials: LB
authors.lastname: Ravi
authors.forename: Joy G
authors.initials: JG
authors.lastname: Mohanty
authors.forename: Francis J
authors.initials: FJ
authors.lastname: Chrest
authors.forename: Rajadas
authors.initials: R
authors.lastname: Jayakumar
authors.forename: Enika
authors.initials: E
authors.lastname: Nagababu
authors.forename: Peter V
authors.initials: PV
authors.lastname: Usatyuk
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Natarajan
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Rifkind
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D016657:Cerebral Amyloid Angiopathy / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D002536:Cerebral Arteries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D016229:Amyloid beta-Peptides