title: A classical live attenuated vaccine for sheep pox.
abstract: A classical live attenuated sheep pox vaccine was prepared using the Ranipet strain of sheep pox virus (SPV) at the 50th passage in a secondary lamb testicular cell system. The TCID50 and RD50 were 10(9.63)/ml and 10(9.51)/ml. respectively. The SID50 of SPV challenge virus was 10(5)/ml. The vaccine was found to have no adverse effects in laboratory animals, and was safe and effective in SPV seronegative lambs. In the field, 660 sheep were vaccinated with an immunizing dose containing 1 x 10(2) TCID50. Randomly selected vaccinated sheep mounted good cell-mediated immunity and humoral responses as measured by glucose utilization test and serum neutralization test, respectively, for the study period of 6 months.
authors.affiliation: Department of Veterinary Microbiology, Veterinary College, University of Agricultural Sciences, HA Farm, Hebbal, Bangalore, Karnataka, India. bhanupraaakash@yahoo.co.in
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.forename: B K
authors.initials: BK
authors.lastname: Indrani
authors.forename: R
authors.initials: R
authors.lastname: Hegde
authors.forename: M M
authors.initials: MM
authors.lastname: Kumar
authors.forename: A R S
authors.initials: AR
authors.lastname: Moorthy
mesh_terms: D000818:Animals; D001831:Body Temperature; D018151:Capripoxvirus / Q000276:immunology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods / Q000662:veterinary; D008297:Male; D051379:Mice; D009500:Neutralization Tests / Q000662:veterinary; D011213:Poxviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary* / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D005947:Glucose