Targeted generation of 16 sequence-tagged sites for bovine chromosome region 5q21-q25 by microdissection.
The cattle chromosome region BTA5q21-q25 is associated with immune defense and is linked with several economically important traits for growth, ovulation, twinning and milk yield. Chromosome microdissection of the region was performed to generate fragment-specific DNA libraries and to provide new bovine-specific DNA sequences as starting material for physical fine-mapping approaches or linkage studies. A first library screening resulted in the identification of 16 region-specific sequence-tagged sites (STS). Fifteen STS represent new unique cattle DNA sequences, of which one identifies a new microsatellite. Homology with the gene CDK2 was found for the remaining STS.
Goldammer
Tom
T
Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
Rottengatter
Karin
K
Weikard
Rosemarie
R
Horstmann
Rolf
R
Gehlhaus
Annette
A
Brunner
Ronald M
RM
Hanotte
Olivier
O
Schwerin
Manfred
M
D000818:Animals; D001483:Base Sequence; D042846:CDC2-CDC28 Kinases / Q000235:genetics; D002417:Cattle; D002875:Chromosomes / Q000235:genetics*; D051357:Cyclin-Dependent Kinase 2; D005260:Female; D015723:Gene Library; D007109:Immunity / Q000235:genetics; D007774:Lactation / Q000235:genetics; D008297:Male; D042282:Microdissection; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010060:Ovulation / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D038522:Twinning, Monozygotic / Q000276:immunology
D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2