title: Use of applied biosystems 7900HT sequence detection system and Taqman assay for detection of quinolone-resistant Neisseria gonorrhoeae.
abstract: Mutations in quinolone resistance-determining regions (QRDRs) have been associated with quinolone-resistant Neisseria gonorrhoeae (QRNG). Since diagnostic nucleic acid amplification tests for gonococci are now in frequent use, molecular detection of QRNG could facilitate surveillance in the absence of culture. Here we describe a real-time molecular assay for detecting QRDR mutations in gonococci.
authors.affiliation: Division of Allergies and Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jgiles4@jhmi.edu [corrected]
authors.forename: Julie
authors.initials: J
authors.lastname: Giles
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Yuenger
authors.forename: Michael
authors.initials: M
authors.lastname: Dan
authors.forename: Karl
authors.initials: K
authors.lastname: Reich
authors.forename: Jonathan
authors.initials: J
authors.lastname: Zenilman
mesh_terms: D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
chemical_list: D002939:Ciprofloxacin