Ovine alpha-amylase genes: isolation, linkage mapping and association analysis with milk traits.
On the basis of comparisons between cattle and sheep genome mapping information the ovine alpha-amylase gene was examined as a possible genetic marker for milk traits in sheep. The objective of the present study was to isolate, map and determine whether this gene is a candidate gene for milk traits. DNA fragments (832 and 2360 bp) corresponding to two different AMY genes were isolated, and one SNP in intron 3 and one GTG deletion in exon 3 of the 2360 bp DNA fragment were found. The 2360 bp ovine AMY DNA fragment was located on chromosome 1 by linkage mapping using the International Mapping Flock. No association was found between estimated breeding values for milk yield, protein and fat contents and AMY genotypes in a daughter design comprising 13 Manchega families with an average of 29 daughters (12-62) per sire.
Calvo
J H
JH
Departamento de Mejora Genética Animal, INIA, 28040 Madrid, Spain. jhcalvo@aragob.es
Marcos
S
S
Beattie
A E
AE
Gonzalez
C
C
Jurado
J J
JJ
Serrano
M
M
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D008892:Milk / Q000737:chemistry*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics*
D017931:DNA Primers; D000516:alpha-Amylases