title: Intracellular localization of VDAC proteins in plants.
abstract: Voltage-dependent anion channels (VDACs) are porin-type beta-barrel diffusion pores. They are prominent in the outer membrane of mitochondria and facilitate metabolite exchange between the organelle and the cytosol. Here we studied the subcellular distribution of a plant VDAC-like protein between plastids and mitochondria in green and non-green tissue. Using in vitro studies of dual-import into mitochondria and chloroplasts as well as transient expression of fluorescence-labeled polypeptides, it could be clearly demonstrated that this VDAC isoform targets exclusively to mitochondria and not to plastids. Our results support the idea that plastids evolved a concept of solute exchange with the cytosol different from that of mitochondria.
authors.affiliation: Department Biologie I, Ludwig-Maximilians-Universität München, Menzingerstr. 67, 80638 München, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Clausen
authors.forename: Iryna
authors.initials: I
authors.lastname: Ilkavets
authors.forename: Rowena
authors.initials: R
authors.lastname: Thomson
authors.forename: Katrin
authors.initials: K
authors.lastname: Philippar
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Vojta
authors.forename: Torsten
authors.initials: T
authors.lastname: Möhlmann
authors.forename: Ekkehard
authors.initials: E
authors.lastname: Neuhaus
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Fulgosi
authors.forename: Jürgen
authors.initials: J
authors.lastname: Soll
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002458:Cell Fractionation; D002736:Chloroplasts / Q000737:chemistry; D016384:Consensus Sequence; D017931:DNA Primers; D008928:Mitochondria / Q000737:chemistry*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018272:Porins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000737:chemistry / Q000166:cytology; D050994:Voltage-Dependent Anion Channels
chemical_list: D017931:DNA Primers; D018272:Porins; D050994:Voltage-Dependent Anion Channels