Extracellular DNA in breast cancer: Cell-surface-bound, tumor-derived extracellular DNA in blood of patients with breast cancer and nonmalignant tumors.
A methylation-specific polymerase chain reaction technique was used to investigate aberrant promoter methylation of RASSF1A and HIC-1 genes in circulating extracellular DNA (exDNA) from the blood of breast cancer and fibroadenoma patients. Methylated DNA could be detected in the exDNA eluted from the surface of erythrocytes and leukocytes, even in the samples where no methylated DNA could be detected in plasma. The data obtained demonstrate that cell surface bound exDNA provides a valuable source of material for early noninvasive cancer diagnostics and monitoring.
Rykova
Elena Yu
EY
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. rykova@niboch.nsc.ru
Laktionov
Pavel P
PP
Skvortsova
Tatyana E
TE
Starikov
Andrey V
AV
Kuznetsova
Nina P
NP
Vlassov
Valentin V
VV
D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D018226:Fibroadenoma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D007962:Leukocytes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics
D004273:DNA, Neoplasm; C096870:Hic1 protein, mouse; D051741:Kruppel-Like Transcription Factors; C411762:RASSF1 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins