Growth and tissue accretion rates of swine expressing an insulin-like growth factor I transgene.
The goal of this research was to determine whether directing expression of an insulin-like growth factor I (IGF-I) transgene specifically to striated muscle would alter the growth characteristics in swine. Transgenic pigs were produced with a fusion gene composed of avian skeletal alpha-actin regulatory sequences and a cDNA encoding human IGF-I. Six founder transgenic pigs were mated to nontransgenic pigs to produce 11 litters of G1 transgenic and sibling control progeny. Birth weight, weaning weight, and proportion of pig survival did not differ between transgenic and control pigs. The ADG of pigs as they grew incrementally from 20 to 60 kg, 60 to 90 kg, and 90 to 120 kg, respectively, did not significantly differ between transgenic and control pigs. Efficiency of feed utilization (gain:feed) was also similar for transgenic and control pigs. Plasma IGF-I and porcine growth hormone (pGH) concentrations were determined at 60, 90, and 120 kg body weight. Plasma IGF-I concentrations were 19% higher in transgenic gilts than control gilts and 11.1% higher in transgenic boars than control boars (P=0.0005). Plasma IGF-I concentrations for boars were also higher than for gilts (P=0.0001). At 60, 90, and 120 kg body weight each pig was scanned by dual energy X-ray absorptiometry (DXA) to derive comparative estimates of carcass fat, lean, bone content of the live animal. Control pigs had more fat and less lean tissue than transgenic pigs at each of the scanning periods and the difference became more pronounced as the pigs grew heavier (P<0.005 at each weight). Transgenic pigs also had a slightly lower percentage of bone than control pigs (P<0.05 at each weight). While daily rates of lean tissue accretion did not differ for transgenic and control pigs, daily rates of fat accretion were lower in transgenic pigs than in control pigs (P<0.05). Based on these results we conclude that expression of IGF-I in the skeletal muscles gradually altered body composition as pigs became older but did not have a major affect on growth performance.
Pursel
V G
VG
Biotechnology and Germplasm Laboratory, USDA-ARS, Beltsville, Maryland 20705, USA. vpursel@anri.barc.usda.gov
Mitchell
A D
AD
Bee
G
G
Elsasser
T H
TH
McMurtry
J P
JP
Wall
R J
RJ
Coleman
M E
ME
Schwartz
R J
RJ
D015502:Absorptiometry, Photon; D000818:Animals; D030801:Animals, Genetically Modified; D000831:Animals, Newborn; D001724:Birth Weight / Q000235:genetics / Q000502:physiology; D001823:Body Composition / Q000235:genetics / Q000502:physiology*; D004435:Eating; D005260:Female; D013006:Growth Hormone / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D013552:Swine / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone