Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission.
Novel ODN probes for insertion polymorphism detection have been devised. The ODN probes labeled with two pyrene chromophores emit strong excimer fluorescence when hybridized with DNA containing one inserted base. This method is useful for the determination of the presence/absence of insertion polymorphisms located at a specific site on the target DNA.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University and SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan.
Ichiba
Tomohisa
T
Saito
Isao
I
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008958:Models, Molecular; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011110:Polymorphism, Genetic; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D011721:Pyrenes; D004247:DNA