title: Evidence for bovine immunodeficiency virus infection in cattle in Zambia.
abstract: We report herein on the first evidence for the presence of bovine immunodeficiency virus (BIV) in Zambia. Serological surveillance of BIV and bovine leukemia virus (BLV) was conducted in traditional cattle herds in Zambia. Out of a total of 262 sera analyzed, 11.4% were found positive for anti-BIV p26 antibodies as determined by Western blot analysis, while 5.0% were positive for anti-BLV gp51 antibodies as detected by immunodiffusion test. Peripheral blood mononuclear cells from BIV seropositive cattle were found to have BIV-provirus DNA, as detected by nested polymerase chain reaction. A nucleotide sequence corresponding to a 298 bp fragment of the BIV pol gene was also analyzed. Amino acid sequences of these Zambian pol gene products showed 98.0 to 100% homology to the American strain BIV R29, 97.0 to 99.0% to Japanese BIV isolates, and divergence ranged from 0.0 to 2.0% among Zambian BIV isolates.
authors.affiliation: Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Sothy
authors.initials: S
authors.lastname: Meas
authors.forename: Megumi
authors.initials: M
authors.lastname: Nakayama
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Usui
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nakazato
authors.forename: Jun
authors.initials: J
authors.lastname: Yasuda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000453:epidemiology / Q000821:virology*; D015751:Genes, pol / Q000235:genetics; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D005779:Immunodiffusion / Q000662:veterinary; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies; D015024:Zambia / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral