Detailed protocol for purification of Chlamydia pneumoniae elementary bodies.
A detailed protocol for the growth and harvest of purified elementary bodies of Chlamydia pneumoniae is presented. This procedure utilizes a flask-to-flask passage scheme designed to achieve high bacterial titers in a short period of time.
Mukhopadhyay
Sanghamitra
S
Division of Infectious Diseases, Department of Medicine, Rm. 311 B Building, 500 South Preston St., University of Louisville School of Medicine, Louisville, KY 40292, USA.
Clark
Alejandra P
AP
Sullivan
Erin D
ED
Miller
Richard D
RD
Summersgill
James T
JT
D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans