title: Abeta production as consequence of cellular death of a human neuroblastoma overexpressing APP.
abstract: In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which overexpress APP; both of them secreted Abeta and showed morphological changes and cell death with apoptotic program characteristics. Interestingly, coculture experiments with the untransfected human neuroblastoma cell line showed that the Abeta peptide was not responsible for the death in those cell lines; additionally, we indicate that upon cell death, Abeta peptide is secreted into cell medium.
authors.affiliation: Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universiadad Autonoma de Madrid, 28049 Cantoblanco, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Recuero
authors.forename: Elena
authors.initials: E
authors.lastname: Serrano
authors.forename: María J
authors.initials: MJ
authors.lastname: Bullido
authors.forename: Fernando
authors.initials: F
authors.lastname: Valdivieso
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D015153:Blotting, Western; D016923:Cell Death; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D009447:Neuroblastoma / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D010455:Peptides / Q000737:chemistry; D013997:Time Factors; D014162:Transfection
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D010455:Peptides