title: Vasopressin phosphorylates HSP27 in aortic smooth muscle cells.
abstract: Administration of arginine vasopressin (AVP) time-dependently induced the phosphorylation of heat shock protein 27 (HSP27) at Ser-15 and Ser-85 in smooth muscle of aorta in vivo. The AVP-induced phosphorylation of HSP27 at Ser-15 and Ser-85 was inhibited by a V1a receptor antagonist but not by a V2 receptor antagonist. In cultured aortic smooth muscle A10 cells, AVP markedly stimulated the phosphorylation of HSP27 at Ser-15 and Ser-85. The AVP-induced phosphorylation of HSP27 was attenuated by SB203580 and PD169316, inhibitors of p38 mitogen-activated protein (MAP) kinase, but not by PD98059, a MEK inhibitor. These results strongly suggest that AVP phosphorylates HSP27 via p38 MAP kinase in aortic smooth muscle cells.
authors.affiliation: Department of Critical Care Medicine, Gifu University School of Medicine, Gifu, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Akamatsu
authors.forename: Keiichi
authors.initials: K
authors.lastname: Nakajima
authors.forename: Akira
authors.initials: A
authors.lastname: Ishisaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsuno
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tanabe
authors.forename: Mariko
authors.initials: M
authors.lastname: Takei
authors.forename: Motoki
authors.initials: M
authors.lastname: Takenaka
authors.forename: Kouseki
authors.initials: K
authors.lastname: Hirade
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshimi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Suga
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Oiso
authors.forename: Kanefusa
authors.initials: K
authors.lastname: Kato
authors.forename: Osamu
authors.initials: O
authors.lastname: Kozawa
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001127:Arginine Vasopressin / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006360:Heat-Shock Proteins; D007093:Imidazoles; D011725:Pyridines; D001127:Arginine Vasopressin; C408604:2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole; C093642:SB 203580