Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen.
OBJECTIVE
To evaluate the effects of animal milk containing fucosylated antigens on Helicobacter pylori (H. pylori) binding to Lewis b antigen.


METHODS
A mammary gland expression vector containing human alpha1-3/4-fucosyltransferase cDNA sequences was constructed. Transient expression of human alpha1-3/4-fucosyltransferase cDNA in goat mammary cell and establishment of transgenic mice were performed. The adhesion inhibitory properties of milk samples were analyzed by using H. pylori.


RESULTS
Goat milk samples were found to inhibit bacterial binding to Lewis b antigen. The highest inhibition was observed 42 h after injection of the plasmid. The binding activity of H. pylori to Lewis b antigen reduced mostly, by 83%, however milk samples from transgenic mice did not inhibit H. pylori binding to Lewis b antigen.


CONCLUSIONS
The use of "humanized" animal milk produced by the transgenic introduction of fucosylated antigen can perhaps provide an alternative therapy and preventive measure for H. pylori infection.
Xu
Hong-Tao
HT
State Key Laboratories for Agrobiotechnology, China Agriculture University, Beijing 100094, China.
Zhao
Yao-Feng
YF
Lian
Zheng-Xing
ZX
Fan
Bao-Liang
BL
Zhao
Zhi-Hui
ZH
Yu
Shu-Yang
SY
Dai
Yun-Ping
YP
Wang
Li-Li
LL
Niu
Hui-Ling
HL
Li
Ning
N
Hammarström
Lennart
L
Borén
Thomas
T
Sjöström
Rolf
R
D000818:Animals; D001422:Bacterial Adhesion; D001789:Blood Group Antigens / Q000378:metabolism*; D005260:Female; D030841:Food, Genetically Modified; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006041:Goats; D016480:Helicobacter pylori / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000201:enzymology; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000201:enzymology / Q000502:physiology*
D001789:Blood Group Antigens; D007983:Lewis Blood Group Antigens; D005647:Fucosyltransferases