title: Hematopoietic progenitor cells residing in muscle engraft into bone marrow following transplantation.
abstract: Hematopoietic and mesenchymal stem cells can potentially be the same cell type or adhere simultaneously in both bone marrow (BM) and muscle. In this study, we asked whether murine BM-derived cells could be tracked in muscle tissue after BM transplantation and whether muscle-derived cells have hematopoietic potential. To answer the first question, we transplanted BM from male BALB/c mice into irradiated female recipients and analyzed for engraftment. We used quantitative polymerase chain reaction (PCR) and fluorescent in situ hybridization techniques for Y chromosome-specific gene probes. A high number of BM-derived cells were located in both the intravascular and extravascular spaces in muscle tissue after BM transplantation. To answer the second question, we analyzed colony-forming potential in vitro with soft-agar assays and the competitive engraftment potential in vivo of muscle-derived cells. Engraftment levels of male cell populations were tested by quantitative PCR. The long-term engraftment potential of muscle-derived cells was low compared with that of BM. We conclude that there is intensive cellular trafficking between BM and muscle tissue. The engraftment potential of muscle-derived stem cells into BM is low and corresponds to the low amounts of hematopoietic colony-forming cells found in muscle tissue.
authors.affiliation: Division of Hematology, Medical Department I, Medical University of Luebeck, Luebeck, Germany.
authors.forename: Kathrin Terres
authors.initials: KT
authors.lastname: Bauermeister
authors.forename: Stephanie
authors.initials: S
authors.lastname: Stölting
authors.forename: Piotr M
authors.initials: PM
authors.lastname: Kaczmarek
authors.forename: Roger
authors.initials: R
authors.lastname: Nadrowitz
authors.forename: Thomas
authors.initials: T
authors.lastname: Wagner
authors.forename: Stefan O
authors.initials: SO
authors.lastname: Peters
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000662:veterinary; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000166:cytology* / Q000637:transplantation; D016133:Polymerase Chain Reaction