Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP.
Daxx-mediated transcriptional repression was modulated by a speckled POZ domain protein SPOP which was first identified as an autoantigen from the serum of a scleroderma patient. This is the first report on the biochemical and functional interactions between Daxx and SPOP. The COOH-terminal region of Daxx interacts with the NH2-terminal region of SPOP. SPOP reversed the transcriptional repression mediated by Daxx which binds with ETS1 transcription factor to repress ETS1-responsive gene expression. Mutagenesis study suggests that the ability of SPOP to self-associate as well as its ability to bind with Daxx was important for the modulation of Daxx-mediated transcriptional repression.
La
Muhnho
M
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, Republic of Korea.
Kim
Karam
K
Park
Jinhwi
J
Won
Jungyeon
J
Lee
Jeung-Hoon
JH
Fu
Ya-Min
YM
Meadows
Gary G
GG
Joe
Cheol O
CO
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000235:genetics*
D009687:Nuclear Proteins; D012097:Repressor Proteins; C509761:SPOP protein, human; D020781:Matrix Metalloproteinase 1