title: [Effect of LHRH crasis protein as vaccine on the reproductive function of male rats].
abstract: OBJECTIVE
  To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and male reproductive function.
  
  
  METHODS
  Trx-LHRH produced in vitro with a new crasis gene which crasised Trx gene and GnRH gene together, was used as vaccine, and hydroalaminum base as adjuvant, in adult SD rats. After 5 weeks of the first treatment, the same dosage was used again to enhance the effect of vaccine. Antibody level was measured by ELISA, and androgen level by RIA.
  
  
  RESULTS
  Trx-LHRH induced successfully the polycolonal antibody at the level of 1 :1 280 approximately 2 560 after 4 weeks of the first treatment, and 1 : 2 000 after 6 weeks of the enhanced treatment. Testosterone level was reduced significantly (P < 0.01) by ELISA, but there was reasonable variation among individuals. Sperm count was also reduced by Trx-LHRH treatment.
  
  
  CONCLUSIONS
  Trx-LHRH can be used as effective vaccine to induce antibody production, and at the same time, restrain the function of hypothatamas-pituitary-testis axis in vivo.
authors.affiliation: Jiangsu Family Planning Institute, Nanjing, Jiangsu 210029, China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Cai
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Pan
authors.forename: Yue
authors.initials: Y
authors.lastname: Jia
authors.forename: Dingzhi
authors.initials: D
authors.lastname: Ma
authors.forename: Xinghai
authors.initials: X
authors.lastname: Wang
authors.forename: Yugui
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013076:Sperm Count; D013739:Testosterone / Q000097:blood; D013879:Thioredoxins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000906:Antibodies; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D013879:Thioredoxins