Maturation of dendritic cells by bacterial immunomodulators.
Dendritic cells (DC) become fully functional upon maturation by various stimuli. We tested whether an immunostimulatory effect of clinically used immunomodulators (Luivac, Biostim, Ribomunyl, Imudon, Bronchovaxom) is caused by direct DC activation. We found that Luivac, Biostim and Ribomunyl have a very high DC stimulatory potential in vitro. The level of DC activation was comparable or higher than DC maturation induced by standard maturation stimuli, Poly (I:C) or lipopolysaccharide. Treated DC had activated phenotype, reduced phagocytic activity and they induced the proliferation of allogeneic T lymphocytes. These results are important for understanding the physiology of action of these widely prescribed agents. Administration of bacterial immunomodulators should be considered with care to avoid the potential risk of inducing an autoimmune disease. They could also be used as well-defined maturating agents in the protocols used for the ex vivo production of DC-based vaccines for clinical trials.
Spisek
Radek
R
Institute of Immunology, 2nd Medical Faculty, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic. radek.spisek@lfmotol.cuni.cz
Brazova
Jitka
J
Rozkova
Daniela
D
Zapletalova
Katerina
K
Sediva
Anna
A
Bartunkova
Jirina
J
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D000954:Antigens, Surface / Q000032:analysis; D001419:Bacteria / Q000737:chemistry*; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects*; D003911:Dextrans; D004705:Endocytosis; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D015203:Reproducibility of Results; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D003470:Culture Media; D016207:Cytokines; D003911:Dextrans; D016753:Interleukin-10; D018664:Interleukin-12; D016650:Fluorescein-5-isothiocyanate