Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizigus marmoreus.
Antiproliferative activities of fractions of Hypsizigus marmoreus were examined using HepG2 cells in vitro. The methanol extract of H. marmoreus markedly induced antiproliferative activity, and an active compound from this mushroom was identified as hypsiziprenol A9. Hypsiziprenol A9 inhibited cell proliferation in a time- and concentration-dependent manner by up to 80% on HepG2 cells by inducing arrest of the G1 phase. Further investigation revealed that hypsiziprenol A9 decreased expression of phosphorylated retinoblastoma protein (ppRb), cyclin D1, and cyclin E in a dose-dependent manner. These results suggest that hypsiziprenol A9 can inhibit the growth of HepG2 cells through inducing G1 phase cell cycle arrest due to the inhibition of pRb phosphorylation.
Chang
Jong-Sun
JS
Department of Food and Nutrition, Yeungnam University, Gyongsan 712-749, South Korea.
Son
Jong-Keun
JK
Li
Gao
G
Oh
Eun-Jung
EJ
Kim
Ji-Young
JY
Park
Sun-Hee
SH
Bae
Joon-Tae
JT
Kim
Hyun-Jeong
HJ
Lee
In-Seon
IS
Kim
Ok-Mi
OM
Kozukue
Nobuyuki
N
Han
Jae-Sook
JS
Hirose
Masao
M
Lee
Kap-Rang
KR
D000363:Agaricales / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D019938:Cyclin D1 / Q000096:biosynthesis; D019927:Cyclin E / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D019927:Cyclin E; D005233:Fatty Alcohols; D016160:Retinoblastoma Protein; C489023:hypsiziprenol A9; D019938:Cyclin D1