Glucocorticoid receptor and Beta-adrenoceptor expression in epididymal adipose tissue from stressed rats.
Adipocytes isolated from epididymal adipose tissue of foot-shock stressed rats are supersensitive to isoprenaline and subsensitive to norepinephrine. These alterations are probably mediated by a stress-induced increase in plasma corticosterone levels. We investigated whether foot-shock stress modifies the expression of glucocorticoid receptors (GRs) and beta-adrenergic protein receptors (beta-ARs) in epididymal adipose tissue from rats submitted to one daily foot-shock session on three consecutive days. This stress protocol caused decreases in GR, beta(1)-AR, and beta(3)-AR protein levels, but caused an increase in beta(2)-AR. These results confirm and support previous functional studies. The alterations in protein expression may be modulated by the high corticosterone levels that downregulate the glucocorticoid receptor.
Farias-Silva
Elisangela
E
Laboratório do Estudo do Estresse (LABEEST), Departamento de Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP: 13089-970, Campinas, SP, Brasil. rspabrat@unicamp.br
dos Santos
Iraídes Nunes
IN
Corezola do Amaral
Maria Esméria
ME
Grassi-Kassisse
Dora Maria
DM
Spadari-Bratfisch
Regina Celia
RC
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004822:Epididymis / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D011965:Receptors, Glucocorticoid