Necessity's sharp pinch.
In a recent issue of Cell, Hughes and coworkers (Mnaimneh et al., 2004) provide a great leap forward in the analysis of essential yeast genes by constructing a strain set that expresses each essential gene from a tetracycline-regulatable promoter.
Chun
Kristin T
KT
Department of Biochemistry and Molecular Biology, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA.
Goebl
Mark G
MG
D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D020043:Genes, Essential; D005800:Genes, Fungal / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*; D014158:Transcription, Genetic
D011500:Protein Synthesis Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D013752:Tetracycline