Cargo recognition during clathrin-mediated endocytosis: a team effort.
Transmembrane proteins destined to endosomes are selectively accumulated in clathrin-coated pits at the plasma membrane and rapidly internalized in clathrin-coated vesicles. The recognition of specific sequence motifs in transmembrane cargo by coated-pit proteins confers specificity on the endocytic process. Interaction of membrane cargo with the clathrin adaptor protein complex AP-2 is the major mechanism of cargo sorting into coated pits in mammalian cells. Recent studies have revealed a variety of alternative mechanisms of cargo recruitment involving additional adaptor proteins. These alternative mechanisms appear to be particularly important during clathrin-mediated endocytosis of signaling receptors.
Sorkin
Alexander
A
Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. alexander.sorkin@uchsc.edu
D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D000818:Animals; D002966:Clathrin / Q000378:metabolism*; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000378:metabolism; D018699:Coated Vesicles / Q000737:chemistry / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D005544:Forecasting; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008954:Models, Biological; D034741:RNA, Small Interfering / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D033962:Adaptor Protein Complex 2; D002966:Clathrin; D034741:RNA, Small Interfering; D014443:Tyrosine; D066246:ErbB Receptors; D007930:Leucine