Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy.
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.
Watarai
Masahisa
M
Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Yamato
Yosuke
Y
Horiuchi
Noriyuki
N
Kim
Suk
S
Omata
Yoshitaka
Y
Shirahata
Toshikazu
T
Furuoka
Hidefumi
H
D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002478:Cells, Cultured / Q000382:microbiology; D045824:Desulfovibrionaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007410:Intestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D020639:Lawsonia Bacteria / Q000276:immunology / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D011817:Rabbits / Q000382:microbiology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; C457280:LsaA protein, Lawsonia intracellularis