Characterization of bro-a gene of Spodoptera litura multicapsid nucleopolyhedrovirus.
Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF120 (designated as the bro-a gene) is a member of the unique multigene family called the baculovirus repeated ORFs (bro) family. Computer-assisted analysis revealed that BRO-A does not contain the conserved Bro-N domain in its N-terminus, but contains a single-stranded DNA binding motif near its C-terminus. Transcriptional analysis indicated that bro-a transcript initiated within a baculovirus consensus early start site motif (ATCAGT). In vitro the bro-a gene transcript was present at 12 h post infection (p.i.) and remained detectable up to 96 h p.i. Western blot analysis of BRO-A expression in SpltMNPV infected S. litura cells showed that it was present from 72 h to 96 h p.i. in vitro. Analysis of extracts from nuclear structures indicated that BRO-A could not be extracted with DNA or proteins which bind to DNA.
Gong
Yingxue
Y
State Key Laboratory for Biocontrol, Institute of Entomology, Zhongshan University, Guangzhou 510275, P.R. China.
Li
Zhaofei
Z
Pan
Lijing
L
Yu
Mei
M
Yang
Kai
K
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera / Q000821:virology*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012333:RNA, Messenger