In vivo accumulation of self-assembling dye Congo red in an area marked by specific immune complexes: possible relevance to chemotherapy.
Supramolecular micellar structures have been proposed as carriers in aim-oriented drug transportation to a target marked by specific immune complexes. In this study, the self-assembling dye Congo red was used as a model supramolecular carrier and its accumulation in the target was studied in vivo. The target was created in vivo as the local specific inflammation provoked by subcutaneous injection of antigen to the ear of a previously immunized rabbit. The color caused by accumulation of Congo red after its intravenous injection was registered by pictures of the ear with suitably filtered visible light shining through it to distinguish Congo red against the background color of hemoglobin. The results confirmed the expected accumulation and retention of Congo red in the inflammation area marked by deposits of specific immune complexes. The role of albumin and its possible interference with transportation of drugs through the blood by supramolecular carriers was also subjected to preliminary examination. The results revealed that albumin collaborates rather than interferes with drug transportation; this is another factor making the use of supramolecular carriers for aim-oriented chemotherapy highly promising.
Rybarska
Janina
J
Institute of Medical Biochemistry, Medical College, Jagiellonian University, Cracow, Poland.
Piekarska
Barbara
B
Stopa
Barbara
B
Spólnik
Paweł
P
Zemanek
Grzegorz
G
Konieczny
Leszek
L
Roterman
Irena
I
D000418:Albumins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism*; D001183:Arthus Reaction / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D003224:Congo Red / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D004358:Drug Therapy / Q000379:methods; D004423:Ear / Q000503:physiopathology*; D007150:Immunohistochemistry; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D011817:Rabbits; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism
D000418:Albumins; D000936:Antigen-Antibody Complex; D004396:Coloring Agents; D012235:Rhodamines; D003224:Congo Red; C029773:rhodamine B