title: [Molecular evolution and geographic origins of type 1 human lymphotrophic virus in Colombia detected by RFLP polymorphism].
abstract: The human T-lymphotropic virus type I (HTLV-I) infection is a public health problem in many endemic areas of Colombia. The subtyping of HTLV-I was based on the analysis of restriction fragment length polymorphisms (RFLP) in 3'LTR proviral DNA. From 31 HTLV-I isolates collected throughout Colombia, a RFLP analysis in a 737 bp 3'LTR fragment was performed. Fifty-eight percent (18/31) were identified as the Cosmopolitan subtype a, 19.4% (6/31) in the West African subtype b, 12.9% (4/31) in the Cosmopolitan subtype b and 9.6% (3/31) in the West African subtype c. The phylogenetic analysis of 3'LTR nucleotide sequences indicated that all the isolates in the current study were in the subgroup B or Japanese, in contrast with the highly divergent isolates from native Amerindians grouped in subgroup a or Transcontinental. The supported hypothesis was that of a post-Columbus introduction of virus represented in the African-American communities of the Colombian South Pacific. Some viral isolates from Colombian native Amerindians exhibited a nucleotide variation compatible with a Paleolithic introduction of the virus. The genetic diversity of HTLV-I in Colombia is complex and probably represents several independent introductions of lymphotropic virus.
authors.affiliation: Laboratorio de Biología Molecular y Patogénesis, Departamento de Ciencias Fisiológicas, Facultad de Salud, Universidad del Valle, Cali, Colombia.
authors.forename: Mónica
authors.initials: M
authors.lastname: Chávez
authors.forename: Martha C
authors.initials: MC
authors.lastname: Domíguez
authors.forename: Abraham
authors.initials: A
authors.lastname: Blank
authors.forename: Milton
authors.initials: M
authors.lastname: Quintana
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Eizuru
authors.forename: Felipe
authors.initials: F
authors.lastname: García
mesh_terms: D001483:Base Sequence; D003105:Colombia / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D019143:Evolution, Molecular; D015490:HTLV-I Infections / Q000208:ethnology* / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral