Transcession of DNA from bacteria to human cells in culture: a possible role in oncogenesis.
The human organism is continuously in close contact with microorganisms, especially bacteria. In the present work, by means of a real-time polymerase chain reaction (PCR) technique, we looked for the presence of a distinct bacterial gene in human cells. To this end, we cultured a human cell line, HL60, in a supernatant in which bacteria (Bacillus subtilis) had been grown. A transient transcession of bacterial DNA into the human cells was observed.
Anker
Philippe
P
Extracellular Biology Research Laboratory, 7 route de Drize, 1227 Carouge/Geneva, Switzerland. panker@worldcom.ch
Zajac
Vladimir
V
Lyautey
Jacqueline
J
Lederrey
Christine
C
Dunand
Christophe
C
Lefort
François
F
Mulcahy
Hugh
H
Heinemann
Jack
J
Stroun
Maurice
M
D001412:Bacillus subtilis / Q000235:genetics; D002467:Cell Nucleus / Q000737:chemistry; D018920:Coculture Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D004270:DNA, Circular / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D018922:HL-60 Cells; D006801:Humans; D009369:Neoplasms / Q000209:etiology*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D004270:DNA, Circular