title: The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?
abstract: In this study we present data on the spatial relationship between neural crest-derived cells (NCC) and the specialized cardiac conduction system (CCS) in the developing murine heart. Using Wnt1-Cre/R26R conditional reporter mice that express beta-galactosidase from ROSA26 upon Cre-mediated recombination, two populations of NCC are seen: one migrates through the arterial pole and contributes to the bundle branches, whereas the second population enters by way of the venous pole and provides cells to the sinoatrial and atrioventricular node areas. The CCS/ lacZ construct is found in the myocardium of the early embryonic heart and afterward only persists in the definitive CCS and is acknowledged as a reporter for the developing conduction system. The contiguous expression of both reporters is suggestive for a potential role of cardiac NCC in the induction of the final differentiation of the CCS.
authors.affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Wassenaarseweg 62, PO 9602, Leiden, The Netherlands. r.e.poelmann@lumc.nl
authors.forename: Robert E
authors.initials: RE
authors.lastname: Poelmann
authors.forename: Monique R M
authors.initials: MR
authors.lastname: Jongbloed
authors.forename: Daniel G M
authors.initials: DG
authors.lastname: Molin
authors.forename: Madelon L
authors.initials: ML
authors.lastname: Fekkes
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Glenn I
authors.initials: GI
authors.lastname: Fishman
authors.forename: Tom
authors.initials: T
authors.lastname: Doetschman
authors.forename: Mohamad
authors.initials: M
authors.lastname: Azhar
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Gittenberger-de Groot
mesh_terms: D000818:Animals; D001283:Atrioventricular Node / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D004627:Embryonic Induction / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006321:Heart / Q000196:embryology* / Q000294:innervation*; D006329:Heart Conduction System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012849:Sinoatrial Node / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D051153:Wnt Proteins; D051155:Wnt1 Protein
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; D051153:Wnt Proteins; D051155:Wnt1 Protein; C495246:Wnt1 protein, mouse