Application of proteomics for discovery of protein biomarkers.
Biomarkers of drug efficacy and toxicity are becoming a key need in the drug development process. Mass spectral-based proteomic technologies are ideally suited for the discovery of protein biomarkers in the absence of any prior knowledge of quantitative changes in protein levels. The success of any biomarker discovery effort will depend upon the quality of samples analysed, the ability to generate quantitative information on relative protein levels and the ability to readily interpret the data generated. This review will focus on the strengths and weaknesses of technologies currently utilised to address these issues.
Hale
John E
JE
Applied Biochemistry Group at Lilly Research Laboratories, IN 46285, USA.
Gelfanova
Valentina
V
Ludwig
James R
JR
Knierman
Michael D
MD
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015195:Drug Design; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*
D015415:Biomarkers