Application of oxidation-reduction assay for monitoring treatment of patients with pulmonary tuberculosis.
By oxidation-reduction assay, the viability of Mycobacterium tuberculosis in sputum specimens was evaluated. The technique is based on the Alamar Blue and Malachite Green dyes, which change their color in response to M. tuberculosis growth. The method is simple, permits visual reading of results, and is applicable for laboratories with limited resources.
Farnia
Parissa
P
Iranian National Reference TB Laboratory, National Research Institute of Tuberculosis and Lung Disease, Shaheed Beheshti University of Medical Sciences & Health Services, Darabad, Tehran 19556, Iran. pfarnia@hotmail.com
Mohammadi
Foroazan
F
Mirsaedi
Mehdi
M
Zarife
Abolhasan Zia
AZ
Tabatabee
Javad
J
Bahadori
Katayoon
K
Bahadori
Muslem
M
Masjedi
Mohammad Reza
MR
Velayati
Ali Akbar
AA
D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development*; D010078:Oxazines; D010084:Oxidation-Reduction; D012394:Rosaniline Dyes / Q000378:metabolism; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000382:microbiology; D014966:Xanthenes
D004396:Coloring Agents; D003470:Culture Media; D010078:Oxazines; D012394:Rosaniline Dyes; D014966:Xanthenes; C005095:malachite green; C005843:resazurin