title: Blood-brain barrier disruption in the hypothalamus of young adult spontaneously hypertensive rats.
abstract: Vascular permeability and endothelial glycocalyx were examined in young adult spontaneously hypertensive rats (SHR), stroke-prone SHR (SHRSP), and Wistar Kyoto rats (WKY) as a control, in order to determine earlier changes in the blood-brain barrier (BBB) in the hypothalamus in chronic hypertension. These rats were injected with horseradish peroxidase (HRP) as an indicator of vascular permeability. Brain slices were developed with a chromogen and further examined with cationized ferritin, a marker for evaluating glycocalyx. Staining for HRP was seen around vessels in the hypothalamus of SHR and SHRSP, but was scarce in WKY. The reaction product of HRP appeared in the abluminal pits of endothelial cells and within the basal lamina of arterioles, showing increased vascular permeability in the hypothalamus of SHR and SHRSP, whereas there were no leaky vessels in the frontal cortex of SHR and SHRSP, or in both areas of WKY. The number of cationized ferritin particles binding to the capillary endothelial cells was decreased in the hypothalamus of SHR and SHRSP, while the number decreased in the frontal cortex of SHRSP, compared with those in WKY. Cationized ferritin binding was preserved in some leaky arterioles, while it was scarce or disappeared in other leaky vessels. These findings suggest that BBB disruption occurs in the hypothalamus of 3-month-old SHR and SHRSP, and that endothelial glycocalyx is markedly damaged there without a close relationship to the early changes in the BBB.
authors.affiliation: Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, 761-0793, Kagawa, Japan. masaueno@med.kagawa-u.ac,jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Liao
authors.forename: Masayuki
authors.initials: M
authors.lastname: Onodera
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Huang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyanaka
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001812:Blood-Brain Barrier / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000502:physiology; D042783:Endothelial Cells / Q000502:physiology; D005293:Ferritins / Q000378:metabolism; D019276:Glycocalyx / Q000502:physiology; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006973:Hypertension / Q000235:genetics / Q000503:physiopathology*; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D008473:Median Eminence; D008854:Microscopy, Electron; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D005293:Ferritins; D006735:Horseradish Peroxidase