Reduced photobleaching of chromophores close to a metal surface.
The photobleaching of chromophores in front of a metal film is measured by recording the emitted fluorescence intensity from an ensemble of chromophores as a function of time. A strong dependence of the photostability on the distance from the metal surface is found. The experimental data are well described in a classical electromagnetic model with the additional assumption that photobleaching occurs at a constant rate from the excited state. The metal interface influences the photostability of the chromophores in two ways, first by altering the excitation rate by local enhancement of the electromagnetic field and second by altering the electromagnetic decay rate.
Vasilev
Krasimir
K
Max Planck Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
Stefani
Fernando D
FD
Jacobsen
Volker
V
Knoll
Wolfgang
W
Kreiter
Maximilian
M
D004574:Electromagnetic Fields; D007700:Kinetics; D008670:Metals / Q000737:chemistry* / Q000494:pharmacology*; D038761:Photobleaching / Q000187:drug effects* / Q000528:radiation effects; D010777:Photochemistry
D008670:Metals