title: Model-based optimization of biosurfactant production in fed-batch culture Azotobacter vinelandii.
abstract: Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cultural liquid and substrate components: glucose, ammonia nitrogen and phosphate phosphorus. The rate of cultural liquid emulsification activity growth as well as the rates of ammonia nitrogen and phosphate phosphorus consumption is modelled by means of artificial neural network, while the rates of the other biochemical transformations are modelled by adequate kinetic relationships.
authors.affiliation: Process Control Department, Kaunas University of Technology, Studentu 50, LT-51368 Kaunas, Lithuania. donatas.levisauskas@ktu.lt
authors.forename: D
authors.initials: D
authors.lastname: Levisauskas
authors.forename: V
authors.initials: V
authors.lastname: Galvanauskas
authors.forename: G
authors.initials: G
authors.lastname: Zunda
authors.forename: S
authors.initials: S
authors.lastname: Grigiskis
mesh_terms: D000465:Algorithms; D016948:Azotobacter vinelandii / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D046508:Culture Techniques; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D015233:Models, Statistical; D008962:Models, Theoretical; D016571:Neural Networks, Computer; D016011:Normal Distribution; D013269:Stochastic Processes; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D005947:Glucose