Peritoneal and splenic B-1 cells are separable by phenotypic, functional, and transcriptomic characteristics.
B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. Although the origin of B-1 cells remains controversial, B-1 cells in different locations are generally considered to be part of the same pool. To determine the validity of this assumption, we examined peritoneal and splenic B-1 cells isolated by flow cytometric cell sorting from normal mice for several features. We found that splenic B-1 cells differ from peritoneal B-1 cells in terms of surface antigen expression, viability ex vivo, immunoglobulin secretion in vitro, stimulated cell cycle progression, and expression of Notch family, Notch-dependent, and Notch-associated genes. These results indicate that splenic and peritoneal B-1 cells are not the same and thus dispute the notion that B-1 cells are uniform, and may suggest that different subpopulations of B-1 cells arise separately, home individually, and/or are heavily influenced by local environmental factors.
Tumang
Joseph R
JR
Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
Hastings
William D
WD
Bai
Chunyan
C
Rothstein
Thomas L
TL
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000145:classification / Q000276:immunology / Q000502:physiology*; D018122:B7-1 Antigen / Q000276:immunology; D005434:Flow Cytometry; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D010537:Peritoneum / Q000276:immunology / Q000502:physiology; D013154:Spleen / Q000276:immunology / Q000502:physiology
D018122:B7-1 Antigen; D007075:Immunoglobulin M