Combining induced mutation and protoplasting for strain improvement of Aspergillus oryzae for kojic acid production.
By combining induced mutation, using NTG and UV irradiation, and protoplasting of a wild type strain of Aspergillus oryzae ATCC 22788, a hyper-producing strain was obtained that accumulated 41 g kojic acid l(-1) in shake-flasks, which was 100-fold higher than that in the wild type strains. Similar production of kojic acid was obtained in 5 l stirred-tank fermentations.
Wan
Hisao-Ming
HM
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30043, Taiwan.
Chen
Chien-Chiang
CC
Chang
Te-Sheng
TS
Giridhar
Raghavan N
RN
Wu
Wen-Teng
WT
D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D016296:Mutagenesis; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism*; D011753:Pyrones / Q000378:metabolism*; D013997:Time Factors; D014466:Ultraviolet Rays
D011753:Pyrones; C011890:kojic acid; D002244:Carbon; D009584:Nitrogen