Use of applied biosystems 7900HT sequence detection system and Taqman assay for detection of quinolone-resistant Neisseria gonorrhoeae.
Mutations in quinolone resistance-determining regions (QRDRs) have been associated with quinolone-resistant Neisseria gonorrhoeae (QRNG). Since diagnostic nucleic acid amplification tests for gonococci are now in frequent use, molecular detection of QRNG could facilitate surveillance in the absence of culture. Here we describe a real-time molecular assay for detecting QRDR mutations in gonococci.
Giles
Julie
J
Division of Allergies and Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jgiles4@jhmi.edu [corrected]
Hardick
Justin
J
Yuenger
Jeffrey
J
Dan
Michael
M
Reich
Karl
K
Zenilman
Jonathan
J
D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
D002939:Ciprofloxacin