title: Characterization of tomato endo-beta-1,4-glucanase Cel1 protein in fruit during ripening and after fungal infection.
abstract: The tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase (EGase) Cel1 protein was characterized in fruit using specific antibodies. Two polypeptides ranging between 51 and 52 kDa were detected in the pericarp, and polypeptides ranging between 49 and 51 kDa were detected in locules. The polypeptides recognized by Cel1 antiserum in fruit are within the size range predicted for Cel1 protein and could be derived from heterogeneous glycosylation. Cel1 protein accumulation was examined throughout fruit ripening. Cel1 protein appears in the pericarp at the stage in which many ripening-related changes start, and remains present throughout fruit ripening. In locules, Cel1 protein is already present at the onset of fruit ripening and remains constant during fruit ripening. This pattern of expression supports a possible role for this EGase in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. The accumulation of Cel1 protein was also analyzed after fungal infection. Cel1 protein and mRNA levels are down-regulated in pericarp after Botrytis cinerea infection but are not affected in locular tissue. The same behavior was observed when fruits were infected with Penicillium expansum, another fungal pathogen. Cel1 protein and mRNA levels do not respond to wounding. These results support the idea that the tomato Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening.
authors.affiliation: Departamento de Genética, Universidad de Valencia, Dr. Moliner 50, Burjassot, 46100 Valencia, Spain.
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Real
authors.forename: Patricia
authors.initials: P
authors.lastname: Company
authors.forename: Pilar
authors.initials: P
authors.lastname: García-Agustín
authors.forename: Alan B
authors.initials: AB
authors.lastname: Bennett
authors.forename: Carmen
authors.initials: C
authors.lastname: González-Bosch
mesh_terms: D020171:Botrytis / Q000472:pathogenicity*; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007700:Kinetics; D018551:Solanum lycopersicum / Q000382:microbiology*; D008970:Molecular Weight; D010935:Plant Diseases / Q000382:microbiology
chemical_list: D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase