Effect of Th1 cytokines on acid secretion in pharmacologically characterised mouse gastric glands.
OBJECTIVE
Acid secretion plays an important role in the ecology of Helicobacter species and acid secretory status heralds patterns of gastritis. The presence of inflammatory cells and their products, in close proximity to parietal cells, questions the extent of the effect of cytokines on acid secretion.


METHODS
We adopted and extensively characterised the mouse gastric gland preparation and its secretory capacity, which was measured using (14)C-aminopyrine accumulation. Subsequently, we tested the secretory properties of a wide range of species specific cytokines, including those associated with Th1 and Th2 immune responses.


RESULTS
(14)C-aminopyrine accumulation in mouse gastric glands was shown to be a very sensitive "in vitro" method of testing classical secretagogues and antisecretory compounds, and provided pharmacological data on acid secretion in the mouse. Only two mouse cytokines, interleukin 2 and interferon gamma, had a direct effect on acid secretion causing dose dependent inhibition.


CONCLUSIONS
Both cytokines belong to the Th1 type immune response and consequently their inhibitory effect may play a role in the hyposecretion seen with H pylori infection and colonisation throughout the corpus of the stomach that potentially can lead to gastric atrophy and subsequently, in some cases, cancer.
Padol
I T
IT
Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada.
Hunt
R H
RH
D000632:Aminopyrine / Q000032:analysis; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D016207:Cytokines / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology; D016480:Helicobacter pylori / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010418:Pentagastrin / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*
D018679:Cholinergic Agonists; D016207:Cytokines; D004791:Enzyme Inhibitors; D005755:Gastrins; D007376:Interleukin-2; D000632:Aminopyrine; D006632:Histamine; D007371:Interferon-gamma; D002217:Carbachol; D010418:Pentagastrin