Neuronal network properties of human teratocarcinoma cell line-derived neurons.
Understanding the structural and functional development of neurons in networks has a high impact to estimate the potentials for restorative therapies. Neurons derived from the human NT2 cell line (hNT) formed networks with a clustered neuritic architecture in vitro, whereas primary dissociated embryonic rat cortical neurons (Cx) displayed a more homogenous cell assembly. Spontaneous spikes of both cell types were recorded on microelectrode arrays within 2 weeks after seeding, but hNT showed a mostly uncorrelated firing pattern in contrast to Cx with highly synchronized bursting. hNT neurons were less sensitive to TTX (IC50 = 5.7 +/- 0.1 nM vs. IC50 = 1.1 +/- 0.2 nM), magnesium (IC50 = 1.83 +/- 0.01 mM vs. IC50 = 0.161 +/- 0.023 mM), and APV (IC50 > 100 microM vs. IC50 = 18 microM). We conclude that embryonic cortical neurons and hNT neurons have different network properties. This should be carefully considered before hNT neurons are used in therapeutic approaches, e g., central nervous system (CNS) grafting.
Görtz
Philipp
P
Department of Neurology, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225, Germany.
Fleischer
Wiebke
W
Rosenbaum
Claudia
C
Otto
Frauke
F
Siebler
Mario
M
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D015415:Biomarkers; D016380:Brain Tissue Transplantation / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008839:Microelectrodes; D009415:Nerve Net / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D018243:Teratocarcinoma; D013779:Tetrodotoxin / Q000494:pharmacology
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D013779:Tetrodotoxin; D015763:2-Amino-5-phosphonovalerate; D008274:Magnesium