Complementary DNA libraries: an overview.
The generation of complete and full-length cDNA libraries for potential functional assays of specific gene sequences is essential for most molecules in biotechnology and biomedical research. The field of cDNA library generation has changed rapidly in the past 10 yr. This review presents an overview of the method available for the basic information of generating cDNA libraries, including the definition of the cDNA library, different kinds of cDNA libraries, difference between methods for cDNA library generation using conventional approaches and a novel strategy, and the quality of cDNA libraries. It is anticipated that the high-quality cDNA libraries so generated would facilitate studies involving genechips and the microarray, differential display, subtractive hybridization, gene cloning, and peptide library generation.
Ying
Shao-Yao
SY
Department of Cell and neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. sying@usc.edu
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic
D017931:DNA Primers; D018076:DNA, Complementary; D011933:Reagent Kits, Diagnostic