title: An improvement of the site-directed mutagenesis method by combination of megaprimer, one-side PCR and DpnI treatment.
authors.affiliation: Department of Microbiology, Nankai University, Tianjin 300071, PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wei
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific