Cytotoxic effects of mansonone E and F isolated from Ulmus pumila.
Two sesquiterpenoids, mansonone E (ME) and mansonone F (MF) were first isolated from the dried root bark of Ulmus pumila (shironire in Japanese), and their antiproliferative activities on human tumor cells were evaluated in vitro. ME had more potent cytotoxic effects on four tumor cell lines, human cervical cancer HeLa, human malignant melanoma A375-S2, human breast cancer MCF-7, and human histiocytic lymphoma U937, than those of MF. The results showed that ME induced oligonucleosomal fragmentation of DNA in HeLa cells and activated caspase-3, followed by the degradation of the inhibitor of caspase-activated DNase, decreased the expression of anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-(XL), and increased that of proapoptotic Bax.
Wang
Dong
D
Department of Pharmacognosy, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University.
Xia
MingYu
M
Cui
Zheng
Z
Tashiro
Shin-ichi
S
Onodera
Satoshi
S
Ikejima
Takashi
T
D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000633:toxicity*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D018517:Plant Roots; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000633:toxicity*; D034403:Ulmus
D000972:Antineoplastic Agents, Phytogenic; D006131:Growth Inhibitors; D009285:Naphthoquinones; D010936:Plant Extracts; D012717:Sesquiterpenes; C496164:mansonone E; C496018:mansonone F