title: T-helper and T-cytotoxic cell subsets monitoring during active cytomegalovirus infection in liver transplantation.
abstract: OBJECTIVE
  The objectives of this study was to analyze the peripheral blood T-lymphocyte subsets of the Th1-related versus Th2-related cytokines of CD4+ cells, and the Tc1 versus Tc2 cytokines of CD8+ cells liver transplant recipients with versus without active cytomegalovirus (CMV) infection.
  
  
  METHODS
  Isolated peripheral blood mononuclear cells (PBMC) were stimulated using PMA/Ionomycin/Monensin. Interleukin (IL)-4 and interferon gamma (IFN-gamma) production by CD4+ and CD8+T cells were determined using fluorescence-activated cell sorter (FACS) analysis.
  
  
  RESULTS
  The ratios of CD4/CD8 were significantly lower among active CMV-infected patients. The levels of Th2 and Tc2 cytokines (IL-4) were similar between CMV-infected and uninfected patients. However, the levels of Th1-type and Tc1-type cytokines (IFN-r) were significantly lower among active CMV-infected patients.
  
  
  CONCLUSIONS
  Low levels of Th1-type cytokines seem to correlate with active CMV infection in liver transplant recipients.
authors.affiliation: Department of Rheumatosis and Biology, Tianjin Institute of Thrombosis and Hemostasis, Organ Transplantation Center of Tianjin First Central Hospital, Tianjin, China. wang yu_l@yahoo.com
authors.forename: Y-L
authors.initials: YL
authors.lastname: Wang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.forename: Y-L
authors.initials: YL
authors.lastname: Zhou
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: Zhu
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: L
authors.initials: L
authors.lastname: Peng
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D015166:Monitoring, Immunologic / Q000379:methods; D011183:Postoperative Complications / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D007166:Immunosuppressive Agents