Affinity parameters of amino acid derivative binding to molecularly imprinted nanospheres consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)].
The binding of L-Boc-phenylalanine anilide (BFA) and L-Boc-phenylalanine (phe) to molecularly imprinted and non-imprinted polymer nanoparticles consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)] has been investigated by adsorption experiments and mathematical modeling. The experimental isotherms have been mathematically adapted following the models of Freundlich, Langmuir, Langmuir-Freundlich, Bi-Langmuir, and extended Langmuir. The extended Langmuir model differentiated between specific and nonspecific binding of the ligand to the receptor nanoparticles and rendered excellent fitting of the experimental data. It delivered a thermodynamic and kinetic parameter set on the experimental association curves of L-BFA by L-BFA-imprinted nanospheres in suspension experiments with the equilibrium constant KD= 4.09 +/- 0.69 micromol L(-1) and the kinetic association rate constant Ka= 5.60 mL micromol(-1) min(-1).
Lehmann
Mathias
M
Fraunhofer Institute for Interfacial Engineering & Biotechnology and Institute for Interfacial Engineering, University of Stuttgart, Nobelstr. 12, 70569 Stuttgart, Germany.
Dettling
Melanie
M
Brunner
Herwig
H
Tovar
Günter E M
GE
D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D007700:Kinetics; D036103:Nanotechnology; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011485:Protein Binding; D013816:Thermodynamics
D000596:Amino Acids; D011109:Polymethacrylic Acids; C497090:poly((ethylene glycol dimethacrylate)-co-(methacrylic acid))