title: Molecular cloning of canine activation-induced cytidine deaminase (AID) cDNA and its expression in normal tissues.
abstract: Activation-induced cytidine deaminase (AID) is essential for class switch recombination, somatic hypermutation, and gene conversion of immunoglobulin gene. In the present study, canine AID cDNA was cloned from the lymph node of a healthy dog by RT-PCR with rapid amplification of cDNA ends (RACE) method. The canine AID cDNA was 1,377 bp in length, and contained the entire open reading frame encoding 198 amino acids which had 94.9%, 94.4%, and 89.9% homology with human, mouse, and chicken homologues, respectively. Canine AID mRNA was expressed in thymus, lung, spleen, kidney, small intestine, lymph node, and tonsil of a healthy dog, similar to humans.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
authors.forename: Taro
authors.initials: T
authors.lastname: Okayama
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D003564:Cytidine Deaminase / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics*; D004285:Dogs; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D018076:DNA, Complementary; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase