Optimization of a liquid chromatography-tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol, matairesinol, lariciresinol, and pinoresinol in foods.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) beta-glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81%, when it was combined with alkaline hydrolysis. Detection limits were 4-10 microg/(100 g dry weight) for solid foods and 0.2-0.4 microg/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33%, respectively. Recovery of lignans added to model products was satisfactory (73-123%), except for matairesinol added to bread (51-55%).
Milder
Ivon E J
IE
RIKILT-Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands. Ivon.Milder@rivm.nl
Arts
Ilja C W
IC
Venema
Dini P
DP
Lasaroms
Johan J P
JJ
Wähälä
Kristina
K
Hollman
Peter C H
PC
D002072:Butylene Glycols / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005663:Furans / Q000032:analysis; D006868:Hydrolysis; D017705:Lignans / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002072:Butylene Glycols; D005663:Furans; D017705:Lignans; C068935:matairesinol; C060282:lariciresinol; C060283:secoisolariciresinol; C103298:pinoresinol