The in vitro quality of washed, prestorage leucocyte-depleted red blood cell concentrates.
OBJECTIVE
No data are currently available on the quality of washed prestorage leucocyte-depleted red blood cell concentrates (RCCs).


METHODS
Five groups of RCCs stored in additive solution (SAG-M) were washed. The groups differed in the age of RCCs (2-5 days or 11-15 days), the temperature during the washing procedure and a 6-h storage period (4 degrees C or room temperature) and the washing solution (saline, SAG-M or 5% albumin). We measured ATP, 2,3-diphosphoglycerate (2,3-DPG), haemolysis, blood cell count, Na(+), K(+), pH, pO(2), pCO(2) and lactate, before and after the washing procedure and hourly during the 6-h postwash storage period.


RESULTS
The erythrocyte ATP content increased by 2-13%, relative to the baseline value, during the washing procedure. The 2,3-DPG level decreased by 15-35% in 2-6-day-old RCCs and by 30-40% in 11-15-day-old RCCs (relative to baseline values) during the washing procedure. In RCCs that were washed and stored at room temperature, and in 2-week-old RCCs, a further decrease in 2,3-DPG of up to 40%, relative to the baseline value, was observed during the 6-h postwash time-period.


CONCLUSIONS
Washing of RCCs stored in SAG-M results in a considerable, significant loss of erythrocyte 2,3-DPG, especially in older RCCs. This loss increases in during a 6-h storage period postwash, even at 4 degrees C. This loss of erythrocyte quality might well outweigh the benefits of washed SAG-M RCCs during massive transfusion in neonates.
Weisbach
V
V
Department of Transfusion Medicine and Haemostaseology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. Volker.Weisbach@trans.imed.uni-erlangen.de
Riego
W
W
Strasser
E
E
Zingsem
J
J
Ringwald
J
J
Zimmermann
R
R
Eckstein
R
R
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000225:Adenine / Q000009:adverse effects / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D001781:Blood Component Removal; D001793:Blood Preservation / Q000379:methods*; D002245:Carbon Dioxide / Q000097:blood; D003080:Cold Temperature; D004905:Erythrocyte Aging; D017707:Erythrocyte Transfusion / Q000009:adverse effects; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D005947:Glucose / Q000009:adverse effects / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006947:Hyperkalemia / Q000139:chemically induced / Q000517:prevention & control; D007231:Infant, Newborn; D007773:Lactates / Q000097:blood; D007962:Leukocytes; D008353:Mannitol / Q000009:adverse effects / Q000494:pharmacology; D010100:Oxygen / Q000097:blood; D011188:Potassium / Q000009:adverse effects / Q000097:blood; D012964:Sodium / Q000097:blood; D012965:Sodium Chloride / Q000009:adverse effects / Q000494:pharmacology; D012996:Solutions / Q000009:adverse effects / Q000494:pharmacology; D013997:Time Factors
C037839:ADSOL; D006454:Hemoglobins; D007773:Lactates; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D002245:Carbon Dioxide; D008353:Mannitol; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D012964:Sodium; D005947:Glucose; D000225:Adenine; D011188:Potassium; D010100:Oxygen