title: Mycobacterial phosphatidylinositol mannoside is a natural antigen for CD1d-restricted T cells.
abstract: A group of T cells recognizes glycolipids presented by molecules of the CD1 family. The CD1d-restricted natural killer T cells (NKT cells) are primarily considered to be self-reactive. By employing CD1d-binding and T cell assays, the following structural parameters for presentation by CD1d were defined for a number of mycobacterial and mammalian lipids: two acyl chains facilitated binding, and a polar head group was essential for T cell recognition. Of the mycobacterial lipids tested, only a phosphatidylinositol mannoside (PIM) fulfilled the requirements for CD1d binding and NKT cell stimulation. This PIM activated human and murine NKT cells via CD1d, thereby triggering antigen-specific IFN-gamma production and cell-mediated cytotoxicity, and PIM-loaded CD1d tetramers identified a subpopulation of murine and human NKT cells. This phospholipid, therefore, represents a mycobacterial antigen recognized by T cells in the context of CD1d.
authors.affiliation: Department of Immunology, Max Planck Institute for Infection Biology, Schumannstrasse 21/22, 10117 Berlin, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Fischer
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Scotet
authors.forename: Marcus
authors.initials: M
authors.lastname: Niemeyer
authors.forename: Heidrun
authors.initials: H
authors.lastname: Koebernick
authors.forename: Jens
authors.initials: J
authors.lastname: Zerrahn
authors.forename: Sophie
authors.initials: S
authors.lastname: Maillet
authors.forename: Robert
authors.initials: R
authors.lastname: Hurwitz
authors.forename: Mischo
authors.initials: M
authors.lastname: Kursar
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneville
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: Ulrich E
authors.initials: UE
authors.lastname: Schaible
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D018949:Antigens, CD1 / Q000378:metabolism*; D055610:Antigens, CD1d; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets; D051379:Mice; D009161:Mycobacterium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000737:chemistry / Q000276:immunology*; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human; D010716:Phosphatidylinositols; C034154:phosphatidylinositol mannoside; D007371:Interferon-gamma