title: Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display.
abstract: SQAG9, a new class of immunosuppressive sulfoquinovosylacylglycerol, and its biotinylated derivatives have been synthesized. A T7 Phage library, composed of random cDNA fragments from Drosophila melanogaster, displayed a possible binding peptide of 14 amino acids. The immobilized synthetic peptide on a sensor chip showed a dissociation constant of K(D)=1.5 x 10(-6) against SQAG9 in a surface plasmon resonance experiment.
authors.affiliation: Genome and Drug Research Center, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Satoko
authors.initials: S
authors.lastname: Aoki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ohta
authors.forename: Shinji
authors.initials: S
authors.lastname: Hyuma
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Fumio
authors.initials: F
authors.lastname: Sugawara
mesh_terms: D000818:Animals; D017123:Bacteriophage T7 / Q000235:genetics*; D004331:Drosophila melanogaster; D006017:Glycolipids / Q000138:chemical synthesis* / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000138:chemical synthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D006017:Glycolipids; D007166:Immunosuppressive Agents; D010455:Peptides; C496611:sulfoquinovosylacylglycerol