The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens.
When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.
Termmathurapoj
Sumeth
S
Department of Pathology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand.
Leelayoova
Saovanee
S
Aimpun
Pote
P
Thathaisong
Umaporn
U
Nimmanon
Thirayost
T
Taamasri
Paanjit
P
Mungthin
Mathirut
M
D000818:Animals; D001483:Base Sequence; D016776:Blastocystis Infections / Q000175:diagnosis* / Q000469:parasitology*; D016845:Blastocystis hominis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D006801:Humans; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling
D016054:DNA, Protozoan