Magnetic resonance angiography and vascular corrosion casting as tools in biomedical research: application to transgenic mice modeling Alzheimer's disease.
In vivo imaging technologies are presently receiving considerable attention in the biomedical and pharmaceutical research areas. One of the principal imaging modalities is magnetic resonance imaging (MRI). The multiparametric nature of MRI enables anatomical, functional and even molecular information to be obtained non-invasively from intact organisms at high spatial resolution. Here we describe the use of one MRI modality, namely angiography (MRA), to non-invasively study the arterial vascular architecture of APP23 transgenic mice modeling Alzheimer's disease. Because the spatial resolution of the technique is limited, the in vivo studies are complemented by a powerful analysis of the vasculature using vascular corrosion casting. Both techniques revealed age-dependent blood flow alterations and cerebrovascular abnormalities in these mice. Our experience suggests that MRA complemented by cast analysis are important tools to describe vascular alterations and test new therapy concepts in animal models of AD. Furthermore, being non-invasive, MRA can also be applied to studies in patients suffering from this disease.
Krucker
Thomas
T
Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA, USA.
Schuler
Alexandra
A
Meyer
Eric P
EP
Staufenbiel
Matthias
M
Beckmann
Nicolau
N
D000375:Aging / Q000502:physiology; D000544:Alzheimer Disease / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D016657:Cerebral Amyloid Angiopathy / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D002539:Cerebral Arterial Diseases / Q000209:etiology* / Q000473:pathology* / Q000503:physiopathology; D002536:Cerebral Arteries / Q000473:pathology* / Q000503:physiopathology; D016319:Corrosion Casting / Q000379:methods* / Q000639:trends; D004195:Disease Models, Animal; D018810:Magnetic Resonance Angiography / Q000379:methods* / Q000639:trends; D051379:Mice; D008822:Mice, Transgenic