Increased pro-thyrotropin-releasing hormone transcription in hypophysiotropic neurons of Lewis rats.
The expression of pro-thyrotropin-releasing hormone (pro-TRH) mRNA was analyzed in the hypothalamus of inflammatory susceptible LEW/N and resistant F344/N rats at baseline and in adrenalectomized and lipopolysaccharide-treated animals. In saline-treated control animals, increased pro-TRH transcription was detected in LEW/N with respect to F344/N rats. This increased LEW/N pro-TRH expression was stable regardless of condition in contrast to F344/N increased pro-TRH transcription post-adrenalectomy and decreased pro-TRH after lipopolysaccharide administration. The LEW/N increase in pro-TRH mRNA was independent of changes in hormonal status suggesting alteration of the thyroid axis at the central level in this strain.
Tonelli
Leonardo H
LH
Section on Neuroendocrine Immunology and Behavior, National Institute of Mental Health, DHHS, 36 Convent Drive MSC 4020, Building 36, Room 1A23, Bethesda, MD 20892-4020, USA.
Gunsolly
Chad A
CA
Belyavskaya
Elena
E
Atwood
Allison R
AR
Sternberg
Esther M
EM
D000315:Adrenalectomy / Q000379:methods; D000704:Analysis of Variance; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D017403:In Situ Hybridization / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D013963:Thyroid Hormones / Q000097:blood; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology
D008070:Lipopolysaccharides; D011498:Protein Precursors; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D013973:Thyrotropin-Releasing Hormone; C046418:pro-thyrotropin releasing hormone; D011761:Pyrrolidonecarboxylic Acid