Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo.
CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.
Hwang
Jungsu
J
Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin 449-701, South Korea.
Kim
Chan Woo
CW
Son
Kyung-No
KN
Han
Kyu Yeon
KY
Lee
Kyung Hee
KH
Kleinman
Hynda K
HK
Ko
Jesang
J
Na
Doe Sun
DS
Kwon
Byoung S
BS
Gho
Yong Song
YS
Kim
Jiyoung
J
D000818:Animals; D001011:Aorta / Q000473:pathology; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D019742:Chemokines, CC; D002642:Chick Embryo; D002823:Chorion / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins; D008297:Male; D015846:Monokines / Q000378:metabolism / Q000502:physiology*; D018919:Neovascularization, Physiologic; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats; D054389:Receptors, CCR1; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
C108814:CCL15 protein, human; C515300:CCR1 protein, human; C515350:CCR3 protein, human; C515302:Ccr1 protein, rat; C515352:Ccr3 protein, rat; D018925:Chemokines; D019742:Chemokines, CC; D004338:Drug Combinations; D007797:Laminin; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D011509:Proteoglycans; D054389:Receptors, CCR1; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; C059485:matrigel; D003094:Collagen