title: The jury is out on "guilt by association" trials.
abstract: The availability of comprehensive protein-protein interaction maps will significantly enhance medical research and aid the functional characterisation of novel genes. To date, the largest scale studies of protein-protein interactions have used the yeast two hybrid method. In this review we take a closer look at the different approaches used in these studies and discuss some key considerations that should be taken into account when designing high throughput interaction mapping projects.
authors.forename: Jennifer I
authors.initials: JI
authors.lastname: Semple
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Sanderson
authors.forename: R Duncan
authors.initials: RD
authors.lastname: Campbell
mesh_terms: D016678:Genome; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020798:Two-Hybrid System Techniques
chemical_list: D010446:Peptide Fragments; D011506:Proteins