title: Expression profiling by high-throughput immunohistochemistry.
abstract: Immunohistochemistry (IHC) provides valuable information on expression of proteins within tissues at a cellular and subcellular level. Recent developments in the practice of IHC now make it possible to contemplate using this technique as a high-throughput expression profiling system. Advances have been made in creation and use of tissue microarrays, in automated IHC and in image capture/analysis. Each of these technologies are reviewed and issues surrounding their use considered. The success of high-throughput IHC is also dependent on both generation and screening of appropriate antibodies. Antibody-related issues which are likely to affect the success of high-throughput IHC, such as specificity, sensitivity, fixation choice, etc., are also considered.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Warford
authors.forename: William
authors.initials: W
authors.lastname: Howat
authors.forename: John
authors.initials: J
authors.lastname: McCafferty
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D000918:Antibody Specificity; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods*; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D051379:Mice
chemical_list: D000906:Antibodies