title: Detailed protocol for purification of Chlamydia pneumoniae elementary bodies.
abstract: A detailed protocol for the growth and harvest of purified elementary bodies of Chlamydia pneumoniae is presented. This procedure utilizes a flask-to-flask passage scheme designed to achieve high bacterial titers in a short period of time.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Rm. 311 B Building, 500 South Preston St., University of Louisville School of Medicine, Louisville, KY 40292, USA.
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: Alejandra P
authors.initials: AP
authors.lastname: Clark
authors.forename: Erin D
authors.initials: ED
authors.lastname: Sullivan
authors.forename: Richard D
authors.initials: RD
authors.lastname: Miller
authors.forename: James T
authors.initials: JT
authors.lastname: Summersgill
mesh_terms: D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans