Expression of a modified Cry1Ie gene in E. coli and in transgenic tobacco confers resistance to corn borer.
The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.
Liu
Yun-Jun
YJ
State Key Laboratory for Agrobiotechnology, National Center for Maize Improvement, China Agricultural University, Beijing.
Song
Fu-Ping
FP
He
Kang-Lai
KL
Yuan
Yuan
Y
Zhang
Xiao-Xia
XX
Gao
Peng
P
Wang
Jian-Hua
JH
Wang
Guo-Ying
GY
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005798:Genes, Bacterial; D007313:Insecta; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D010947:Plants, Toxic / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*
D025801:Ubiquitin