[The protective effect of amifostine on hydroquinone-induced apoptosis in bone marrow].
OBJECTIVE
To evaluate the protective effect of amifostine on hydroquinone-induced apoptosis of bone marrow mononuclear cells in vitro.


METHODS
The mononuclear cells were separated and divided into four groups: blank control, amifostine group, hydroquinone group, amifostine + hydroquinone group. The cell apoptotic rate was examined in separated group at different time point, and apoptosis was detected by HT stain, then cell morphology was observed under fluorescent microscope and DNA fragments was tested by agarose gel electrophoresis. In addition, apoptotic and necrotic rate was detected by flow cytometer.


RESULTS
After 10 hour culture, DNA ladder was detected in the hydroquinone group, but not in other groups. The apoptotic rate was not significantly different between amifostine group and blank control group at different culture time (P > 0.05). After 8 - 12 hour culture, the apoptotic rate in amifostine + hydroquinone group was significantly lower than that in the group of hydroquinone alone (P < 0.01). After 18 - 48 hour culture, the necrotic rate in amifostine + hydroquinone group was lower than that in the group of hydroquinone alone (P < 0.05).


CONCLUSIONS
Amifostine can protect cell from hydroguinone-induced bone marrow damage through inhibition on cell apoptosis, and decrease in cell necrosis.
Chen
Yi
Y
Department of Hematology, Wenzhou Medical College, Wenzhou 325000, China.
Yu
Kang
K
Wu
Jian-bo
JB
Zhang
Jun-li
JL
Hu
Xu-dong
XD
Jiang
Lei
L
Zhang
Sheng-hui
SH
Hu
Xiao-xia
XX
Gao
Shen-meng
SM
D004999:Amifostine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D006873:Hydroquinones / Q000037:antagonists & inhibitors*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D020011:Protective Agents / Q000494:pharmacology
D006873:Hydroquinones; D020011:Protective Agents; D004999:Amifostine; C031927:hydroquinone