title: L-proline is essential for the intracellular differentiation of Trypanosoma cruzi.
abstract: Using as the host cell, a proline-requiring mutant of Chinese hamster ovary cell (CHO-K1), it was possible to arrest the differentiation of amastigote forms of Trypanosoma cruzi at the intermediate intracellular epimastigote-like stage. Complete differentiation to the trypomastigote stage was obtained by addition of L-proline to the medium. This effect was more pronounced using the T. cruzi CL-14 clone that differentiates fully at 33 degrees C (permissive temperature) and poorly at 37 degrees C (restrictive temperature). A synchronous differentiation of T. cruzi inside the host-cell is then possible by temperature switching in the presence of proline. It was found that differentiation of intracellular epimastigotes and trypomastigote bursting were proline concentration dependent. The intracellular concentration of proline was measured as well as the transport capacity of proline by each stage of the parasite. Amastigotes have the highest concentration of free proline (8.09 +/- 1.46 mM) when compared to trypomastigotes (3.81 +/- 1.55) or intracellular epimastigote-like forms (0.45 +/- 0.06 mM). In spite of having the lowest content of intracellular free proline, intracellular epimastigotes maintained the highest levels of L-proline transport compared to trypomastigotes and intracellular amastigotes, providing evidence for a high turnover for the L-proline pool in that parasite stage. This is the first report to establish a relationship between proline concentration and intracellular differentiation of Trypanosoma cruzi in the mammalian host.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, 05513-970, Brazil.
authors.forename: Renata R
authors.initials: RR
authors.lastname: Tonelli
authors.forename: Ariel M
authors.initials: AM
authors.lastname: Silber
authors.forename: Marinez
authors.initials: M
authors.lastname: Almeida-de-Faria
authors.forename: Izaura Y
authors.initials: IY
authors.lastname: Hirata
authors.forename: Walter
authors.initials: W
authors.lastname: Colli
authors.forename: Maria Júlia M
authors.initials: MJ
authors.lastname: Alves
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D011392:Proline / Q000494:pharmacology / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D011392:Proline