title: Analysis of the sulfur mustard metabolite 1,1'-sulfonylbis[2-S-(N-acetylcysteinyl)ethane] in urine by negative ion electrospray liquid chromatography- tandem mass spectrometry.
abstract: A method is described for the analysis of the sulfur mustard metabolite 1,1'-sulfonylbis[2-S-(N-acetylcysteinyl)ethane] in human urine. The analyte was concentrated from urine on a polymeric SPE cartridge and analyzed by liquid chromatography-negative ion electrospray tandem mass spectrometry in the selected reaction monitoring mode. The limit of detection was 0.5-1 ng/mL. The metabolite was detected at concentrations close to the detection limit in samples of urine from two casualties accidentally exposed to sulfur mustard from a First World War munition.
authors.affiliation: Defence Science and Technology Laboratory, Porton Down, Salisbury, SP4 0JQ, United Kingdom.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Read
authors.forename: Robin M
authors.initials: RM
authors.lastname: Black
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine*; D002619:Chemical Warfare Agents / Q000506:poisoning*; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D009151:Mustard Gas / Q000506:poisoning*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C496170:1,1'-sulfonylbis(2-S-(N-acetylcysteinyl)ethane); D002619:Chemical Warfare Agents; D009151:Mustard Gas; D000111:Acetylcysteine