title: Human immune responses to porcine endogenous retrovirus-derived peptides presented naturally in the context of porcine and human major histocompatibility complex class I molecules: implications in xenotransplantation of porcine organs.
abstract: BACKGROUND
  Porcine endogenous retroviruses (PERV) have been shown to infect human cells, raising concerns regarding safety of xenotransplantation. In patients exposed to porcine tissues, no PERV infection has been observed. This study was designed to develop human CD8+ cytotoxic T lymphocytes (CTL) against PERV-derived peptides presented in the context of human leukocyte antigen (HLA) or swine leukocyte antigen (SLA) class I molecules and to define dominant epitopes contributed by PERV.
  
  
  METHODS
  Human CD8+ CTL were generated against porcine aortic endothelial cells (PAEC). Peptides presented on SLA class I molecules were acid eluted and fractionated by reverse-phase high-performance liquid chromatography. Peptide fractions that restored lysis of acid-stripped PAEC were sequenced by tandem mass spectrometry. Human CD8+ CTL were generated against PERV envelope-derived peptides and PERV-infected human cells to identify immunodominant PERV-derived epitopes.
  
  
  RESULTS
  We identified two peptides derived from retroviral transactivating regulatory protein (AHQDPLPEQP) and retroviral transcription factor (PQKPFVT) recognized by human CD8+ CTL in the context of SLA class I. Computer-assisted analysis identified nine PERV-envelope-derived 9-mer peptides with high affinity for the HLA-A2 molecule (Env-1-9). PERV-specific CD8+ CTL generated in vitro identified the immunodominant Env-5 peptide (303-311, KLFSLIQGA) and demonstrated HLA-A2-restricted cytotoxicity against PERV-infected human cells.
  
  
  CONCLUSIONS
  Our results indicate that PERV-derived peptides are presented naturally on porcine and human major histocompatibility complex class I molecules. CD8+ CTL responses elicited against dominant SLA and HLA class I-restricted PERV-derived epitopes may play an important role in xenograft rejection and in containment of PERV infection of human cells after xenotransplantation.
authors.affiliation: Department of Surgery, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
authors.forename: Sabarinathan
authors.initials: S
authors.lastname: Ramachandran
authors.forename: Andrés
authors.initials: A
authors.lastname: Jaramillo
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Xu
authors.forename: Brice W
authors.initials: BW
authors.lastname: McKane
authors.forename: William C
authors.initials: WC
authors.lastname: Chapman
authors.forename: T
authors.initials: T
authors.lastname: Mohanakumar
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001011:Aorta / Q000166:cytology / Q000276:immunology; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology; D000939:Epitopes; D015789:HLA-A2 Antigen / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D006801:Humans; D016056:Immunodominant Epitopes; D016377:Organ Transplantation; D010455:Peptides / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D012190:Retroviridae / Q000378:metabolism*; D013552:Swine / Q000821:virology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014183:Transplantation, Heterologous; D014759:Viral Envelope Proteins / Q000737:chemistry
chemical_list: D000939:Epitopes; D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C438668:swine leukocyte antigen