Production of transgenic chimeric rabbits and transmission of the transgene through the germline.
Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras.
Bodó
Szilárd
S
Department of Animal Biology, Agricultural Biotechnology Center, Gödöllö, Hungary.
Gócza
Elen
E
Révay
Tamás
T
Hiripi
László
L
Carstea
Bogdan
B
Kovács
András
A
Bodrogi
Lilla
L
Bösze
Zsuzsanna
Z
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D046528:Chimerism; D002874:Chromosome Mapping; D017931:DNA Primers; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D018095:Germ-Line Mutation; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011817:Rabbits; D013480:Superovulation
D017931:DNA Primers; C078147:F8 protein, human; D005169:Factor VIII