Identification of phalloidin uptake systems of rat and human liver.
To determine whether the liver toxin phalloidin is transported into hepatocytes by one of the known bile salt transporters, we expressed the sodium-dependent Na+/taurocholate cotransporting polypeptide (Ntcp) and several sodium-independent bile salt transporters of the organic anion transporting polypeptide (OATP/SLCO) superfamily in Xenopus laevis oocytes and measured uptake of the radiolabeled phalloidin derivative [3H]demethylphalloin. We found that rat Oatp1b2 (previously called Oatp4 (Slc21a10)) as well as human OATP1B1 (previously called OATP-C (SLC21A6)) and OATP1B3 (previously called OATP8 (SLC21A8)) mediate uptake of [3H]demethylphalloin when expressed in X. laevis oocytes. Transport of increasing [3H]demethylphalloin concentrations was saturable with apparent Km values of 5.7 microM (Oatp1b2), 17 microM (OATP1B1) and 7.5 microM (OATP1B3). All other tested Oatps/OATPs as well as the rat liver Ntcp did not transport [3H]demethylphalloin. Therefore, we conclude that rat Oatp1b2 as well as human OATP1B1 and OATP1B3 are responsible for phalloidin uptake into rat and human hepatocytes.
Meier-Abt
Fabienne
F
Department of Medicine, Division of Clinical Pharmacology and Toxicology, University Hospital, Ramistr. 100, CH-8091, Zurich, Switzerland.
Faulstich
Heinz
H
Hagenbuch
Bruno
B
D000818:Animals; D000838:Anions; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D027361:Organic Anion Transporters / Q000502:physiology; D029362:Organic Anion Transporters, Sodium-Independent / Q000502:physiology; D010455:Peptides / Q000737:chemistry; D010590:Phalloidine / Q000493:pharmacokinetics*; D051381:Rats; D012964:Sodium / Q000737:chemistry / Q000378:metabolism; D013656:Taurocholic Acid / Q000493:pharmacokinetics; D014982:Xenopus laevis
D000838:Anions; D027361:Organic Anion Transporters; D029362:Organic Anion Transporters, Sodium-Independent; D010455:Peptides; C421102:Slco1a1 protein, rat; D010590:Phalloidine; D013656:Taurocholic Acid; D012964:Sodium