title: [Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis].
abstract: OBJECTIVE
  To observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.
  
  
  METHODS
  Semen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.
  
  
  RESULTS
  Sperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters, including average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), straightness (STR) and linearity (LIN), except heat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP, VSL and VCL) and moving pattern parameters (ALH) significantly decreased, while that of LIN and STR significantly increased in post-thaw samples.
  
  
  CONCLUSIONS
  CASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples.
authors.affiliation: Human Semen Bank, Guangdong Family Planning Hospital, Guangzhou, Guangdong 510600, China. dxlmyi@pub.guangzhou.gd.cn
authors.forename: Meiyi
authors.initials: M
authors.lastname: Liu
authors.forename: Shunmei
authors.initials: S
authors.lastname: Deng
authors.forename: Chunjie
authors.initials: C
authors.lastname: Ma
authors.forename: Aiping
authors.initials: A
authors.lastname: Chen
authors.forename: Yanjia
authors.initials: Y
authors.lastname: Jiang
authors.forename: Renqian
authors.initials: R
authors.lastname: Wen
authors.forename: Qiling
authors.initials: Q
authors.lastname: Wang
authors.forename: Lixin
authors.initials: L
authors.lastname: Tang
authors.forename: Jianchu
authors.initials: J
authors.lastname: Huang
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Yao
mesh_terms: D015925:Cryopreservation; D003936:Diagnosis, Computer-Assisted; D005615:Freezing; D006801:Humans; D008297:Male; D013081:Sperm Motility