Mutagenicity of sediment and biomarkers of oxidative stress in fish from aquatic environments under the influence of tanneries.
The mutagenicity of interstitial water and organic extracts from the sediments in the Cadeia and Feitoria Rivers, RS, Brazil, were evaluated by Salmonella microsuspension bioassay using TA97a, TA98, TA100 and TA102 strains, in the absence and presence of S9 mix. At the contaminated site, the mutagenic responses for interstitial water, suggested the presence of frameshift and base pair substitution mutagens, including oxidative substances. Organic extracts presented direct or indicative mutagenesis to the TA97a, TA98 and TA100 strains. In general, an exogenous metabolic systems decreased the mutagenicity of the samples. High concentrations of total chromium found in the sediment and interstitial water as well as total mercury in the sediment of the contaminated site, when compared to the control area, may help explain the mutagenic results. The livers of Gymnogeophagus gymnogenys collected in this impacted area, compared to a non-polluted site, were analyzed for oxidative stress parameters. Compared to the controls, there was a significant increase in the activity of superoxide dismutase (SOD) at levels of substances reactive to thiobarbituric acid (TBARS), and in the chemiluminescence of hepatic cells in fish in the polluted area. The concentration of cytochromes P450 and b5 decreased drastically in the fish at the polluted site, while the catalase activity did not change. It was possible to correlate the biological changes in the fish with the presence of mutagenic compounds in sediment and interstitial water in this area.
Tagliari
Kelly Cristina
KC
Departamento de Ciências Patológicas, Campus Universitário, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid, PR445, KM380, Caixa Postal 6001, 86051-990 Londrina, PR, Brazil.
Cecchini
Rubens
R
Rocha
Jocelita Aparecida Vaz
JA
Vargas
Vera Maria Ferrão
VM
D000818:Animals; D001681:Biological Assay; D001938:Brazil; D002374:Catalase / Q000378:metabolism; D023681:Cichlids / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D019015:Geologic Sediments / Q000032:analysis*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008163:Luminescent Measurements; D019216:Metals, Heavy / Q000032:analysis; D009152:Mutagenicity Tests; D018384:Oxidative Stress / Q000187:drug effects*; D045483:Rivers / Q000737:chemistry*; D012475:Salmonella / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis; D013633:Tanning; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D019216:Metals, Heavy; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D002374:Catalase; D013482:Superoxide Dismutase