Isolation of Rhodococcus equi from the feces of indigenous animals and soil from the Lower Zambezi National Park and Lochinvar National Park, Zambia.
Rhodococcus equi is an important pathogen in foals; however, its incidence in African indigenous animals is poorly understood. Fecal samples (92 from nine indigenous species) and 43 soil samples were collected from two Zambian National Parks. The presence of R. equi was investigated and 533 isolates were tested for the presence of 15- to 17-kDa antigens (VapA) and a 20-kDa antigen (VapB) by immunoblotting and PCR. R. equi was isolated (10(2)-10(4) colony forming units/g) from 75% of fecal and 74% of soil samples. Neither antigen was detected; however, about 20% of the isolates contained cryptic plasmids of various sizes. There was no evidence of virulent R. equi, but the avirulent form was widespread in the animals and the soil.
Takai
Shinji
S
Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Syakalima
Michelo
M
Yasuda
Jun
J
Sasaki
Yukako
Y
Tsutsumi
Hisako
H
Miyagawa
Emiko
E
Wada
Kaya
K
Kakuda
Tsutomu
T
Tsubaki
Shiro
S
Sugimoto
Chihiro
C
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D002020:Buffaloes; D004269:DNA, Bacterial / Q000032:analysis; D004606:Elephants; D018397:Equidae; D005243:Feces / Q000382:microbiology*; D006736:Horses; D015151:Immunoblotting / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016929:Rhodococcus equi / Q000302:isolation & purification*; D012377:Rodentia; D012988:Soil Microbiology; D013552:Swine; D015024:Zambia / Q000453:epidemiology
D004269:DNA, Bacterial