Influence of osteoprotegerin (OPG) on resorption of heterotopically induced ossicle.
In this paper, the effect of osteoprotegerin (OPG) on slowing down the resorption process of heterotopically induced bone tissue is described. The induced ossicle is resorbed ex inactivitate. This system mimics osteoporosis in immobilised skeletal bones. Bone induction was achieved in BALB/c mice after the injection of the suspension of 3 x 10(6) HeLa cells into thigh muscle of animals immuno-suppressed by a single dose of hydrocortisone. To slow down the process of resorption we applied OPG and measured quantitatively the effect by weighing the mass of mineral deposited in the induced ossicle after hydrolysis of soft tissues surrounding the induced ossicles. As the effect of application of OPG more than 340-540% of bone mineral is found in the induced ossicles following nine applications of 0.05 mg OPG per mouse, every second day--in comparison to the control animals.
Kochanowska
Iwona
I
Institute for Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Włodarski
Krzysztof
K
Pienkowski
Marek
M
Ostrowski
Kazimierz
K
D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000473:pathology; D017690:Cell Transplantation; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis; D010024:Osteoporosis / Q000188:drug therapy / Q000473:pathology; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; D014183:Transplantation, Heterologous
D006023:Glycoproteins; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; C506031:Tnfrsf11b protein, mouse