The long-wavelength chlorophyll states of plant LHCI at room temperature: a comparison with PSI-LHCI.
The red antenna states of the external antenna complexes of higher plant photosystem I, known as LHCI, have been analyzed by measurement of their preequilibrium fluorescence upon direct excitation at 280 K. In addition to the previously detected F735 state, a hitherto undetected low-energy state with emission maximum around 713 nm was observed. The 280 K bandwidths (FWHM) are 55 nm for the F735 state and approximately 27 nm for the F713-nm state, much greater than for non-red-shifted antenna chlorophylls. The origin absorption band for the F735-nm state was directly detected by determination of its excitation (action) spectrum and lies at 709-710 nm. The absorption spectrum for F735, calculated using the Stepanov expression, closely overlaps the excitation spectrum, indicating that the very large Stokes shift (25 nm) is due to vibrational relaxation within the excited-state manifold and solvent effects can be excluded. Fluorescence anisotropy measurements, with direct excitation of F735, indicate that the transition dipoles of the two red states are parallel. Similar experiments performed in the long-wavelength absorbing tail of PSI-LHCI indicate the presence of emission state(s) that are red-shifted with respect to F735 of isolated LHCI. It is suggested that these are brought about by interactions between the complexes in PSI-LHCI, which occur in some yet undefined way, and which are broken upon solubilization of the component parts.
Jennings
Robert C
RC
Istituto di Biofisica del Consiglio Nazionale delle Ricerche, Sezione di Milano, Dipartimento di Biologia, Università degli Studi di Milano, Milan, Italy. robert.jennings@unimi.it
Zucchelli
Giuseppe
G
Engelmann
Enrico
E
Garlaschi
Flavio M
FM
D002734:Chlorophyll / Q000502:physiology*; D045342:Light-Harvesting Protein Complexes / Q000502:physiology*; D045331:Photosystem I Protein Complex / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D020524:Thylakoids / Q000502:physiology*; D003313:Zea mays / Q000502:physiology
D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D002734:Chlorophyll