Evaluation of the new heterogeneous ACMIA immunoassay for the determination of whole-blood cyclosporine concentrations in bone marrow, kidney, heart, and liver transplant recipients.
Cyclosporine (CyA) has a narrow therapeutic index. Determination of CyA concentrations correlate with rejection or adverse effects like nephropathy. Cyclosporine is assayed based on either chromatographic or many different immunoenzymologic techniques. The investigators evaluated a new heterogeneous immunoassay of CyA on RxL Dimension. The pretreatment step is automatically performed in the apparatus. Linearity, intra- and interday precision, limit of quantification, dilutions, and stability of the equipment were compared with the EMIT method for patient determinations. The heterogeneous immunoassay showed a good linearity between 0 and 500 ng/mL, and intra- and inter-day precision with a coefficient of variation below 9.2%. The investigators observed reproducible and accurate dilutions of high concentrations (500 to 2000 ng/mL). The correlation with the EMIT technique was valid: ACMIA = 0.964 EMIT + 0.156 (r = .96) for different types of transplant (n = 116). Finally, this new system improves the determination of CyA concentrations.
Huet
E
E
Laboratory of Pharmacology and Toxicology, CHU Henri Mondor, 51 ave du mal de Lattre de Tassigny, 94010 Creteil Cédex, France.
Morand
K
K
Blanchet
B
B
Astier
A
A
Hulin
A
A
D016026:Bone Marrow Transplantation / Q000276:immunology*; D016572:Cyclosporine / Q000097:blood*; D016903:Drug Monitoring / Q000379:methods; D017248:Enzyme Multiplied Immunoassay Technique; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007166:Immunosuppressive Agents / Q000097:blood; D016030:Kidney Transplantation / Q000276:immunology*; D016031:Liver Transplantation / Q000276:immunology*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007166:Immunosuppressive Agents; D016572:Cyclosporine