title: Comparison of human skeletal myoblasts and bone marrow-derived CD133+ progenitors for the repair of infarcted myocardium.
abstract: OBJECTIVE
  The present study was designed as a face-to-face functional comparison of human skeletal myoblasts (SMs) and CD133(+) bone marrow-derived hematopoietic progenitors in an animal model of semichronic myocardial infarction.
  
  
  BACKGROUND
  Compared with SMs, bone marrow-derived cells have the advantage of plasticity and might more effectively regenerate ischemic cardiac tissue. However, few data exist on the comparative efficacy of these two cell types in semichronic infarcts.
  
  
  METHODS
  A myocardial infarction was created by coronary ligation in 32 nude rats. Ten days later, rats received in-scar injections of human SMs, CD133(+) progenitors, or culture medium. Left ventricular function was assessed before and one month after transplantation by echocardiography and pressure-volume loops. Immunofluorescence, polymerase chain reaction, and in situ hybridization were used to detect cells grafted in the hearts.
  
  
  RESULTS
  One month after transplantation, left ventricular ejection fraction decreased by 8 +/- 4% in controls, whereas it increased by 7 +/- 3% in CD133(+)-grafted hearts (p = 0.0015 vs. controls) and further by 15 +/- 5% in SM-treated hearts (p = 0.008 vs. controls). Systolic indices yielded by pressure-volume loops paralleled these data. Engrafted myotubes were identified in all SM-treated hearts by immunofluorescence, whereas in CD133(+)-grafted hearts, few human cells were only detected by polymerase chain reaction.
  
  
  CONCLUSIONS
  In the setting of postinfarction scars, the transplantation of bone marrow-derived CD133(+) progenitors improves cardiac function, but this benefit is not superior to that afforded by myogenic cells.
authors.affiliation: Inserm U572, Hôpital Lariboisiére, Paris, France. onnik.agbulut@larib.inserm.fr
authors.forename: Onnik
authors.initials: O
authors.lastname: Agbulut
authors.forename: Susanne
authors.initials: S
authors.lastname: Vandervelde
authors.forename: Nawwar
authors.initials: N
authors.lastname: Al Attar
authors.forename: Jérôme
authors.initials: J
authors.lastname: Larghero
authors.forename: Said
authors.initials: S
authors.lastname: Ghostine
authors.forename: Bertrand
authors.initials: B
authors.lastname: Léobon
authors.forename: Estelle
authors.initials: E
authors.lastname: Robidel
authors.forename: Paolo
authors.initials: P
authors.lastname: Borsani
authors.forename: Marc
authors.initials: M
authors.lastname: Le Lorc'h
authors.forename: Alvine
authors.initials: A
authors.lastname: Bissery
authors.forename: Christine
authors.initials: C
authors.lastname: Chomienne
authors.forename: Patrick
authors.initials: P
authors.lastname: Bruneval
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Marolleau
authors.forename: Jean-Thomas
authors.initials: JT
authors.lastname: Vilquin
authors.forename: Albert
authors.initials: A
authors.lastname: Hagège
authors.forename: Jane-Lyse
authors.initials: JL
authors.lastname: Samuel
authors.forename: Philippe
authors.initials: P
authors.lastname: Menasché
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000032:analysis; D003114:Colony-Forming Units Assay; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D006085:Graft Survival; D006321:Heart / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D032448:Myoblasts, Skeletal / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000166:cytology; D010455:Peptides / Q000032:analysis*; D016133:Polymerase Chain Reaction; D051381:Rats; D011923:Rats, Nude; D012038:Regeneration; D013318:Stroke Volume; D016277:Ventricular Function, Left
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000954:Antigens, Surface; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606025:Prom1 protein, rat