Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.
Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.
Grigolo
B
B
Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli Bologna, Bologna.
Lisignoli
G
G
Piacentini
A
A
Fiorini
M
M
Roseti
L
L
De Franceschi
L
L
Tognana
E
E
Pavesio
A
A
Facchini
A
A
D000293:Adolescent; D000328:Adult; D002356:Cartilage / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D023822:Tissue Engineering / Q000379:methods
C086358:hyaluronic acid benzyl ester; D006820:Hyaluronic Acid