[Plasma assay of methadone enantiomers with high performance liquid chromatography].
6-Dimethylamino-4,4-diphenylheptane-3-one (methadone) is a synthetic opioid. Presence of an assymetrical carbon in its structure explains existence of two enantiomers. Levogyral enantiomer or R-methadone exhibits an 25 fold superior analgesic activity to the dextrogyral enantiomer S-methadone. In order to run separately a plasma assay of these two enantiomers, a chromatographic chiral separation method coupled with an ultraviolet detection has been performed. It allows selective assay of each enantiomer. This method, although an analytical interference with d-propoxyphene, is sensitive, reproductible, specific and shows a convenient resolution for the analysis of both the stereospecific and racemic forms. This method can be applied for pharmacokinetic study of the drug in patients treated by methadone.
Batista
R
R
Service de pharmacie - pharmacologie - toxicologie, Hôtel-Dieu, 1, place du Parvis Notre-Dame, F-75181 Paris Cedex 04.
Badré-Sentenac
S
S
Bardin
C
C
Chast
F
F
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008691:Methadone / Q000097:blood*; D008875:Middle Aged; D009294:Narcotics / Q000097:blood*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D009294:Narcotics; D008691:Methadone