title: Enhancing linkage analysis of complex disorders: an evaluation of high-density genotyping.
abstract: To explore the potential value of recently developed high-density linkage mapping methods in the analysis of complex disease we have regenotyped five nuclear families first studied in the 1996 UK multiple sclerosis linkage genome screen, using Applied Biosystems high-density microsatellite linkage mapping set, the Illumina BeadArray linkage mapping panel (version 3) and the Affymetrix GeneChip Human Mapping 10K array. We found that genotyping success, information extraction and genotyping accuracy were improved with all systems. These improvements were particularly marked with the SNP-based methods (Illumina and Affymetrix), with little difference between these. The extent of additional information extracted is considerable, indicating that reanalysis of existing multiplex families using these newer systems would substantially increase power.
authors.affiliation: University of Cambridge Neurology Unit, Addenbrooke's Hospital, UK. sjs1016@mole.bio.cam.ac.uk
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Sawcer
authors.forename: Mel
authors.initials: M
authors.lastname: Maranian
authors.forename: Sara
authors.initials: S
authors.lastname: Singlehurst
authors.forename: TaiWai
authors.initials: T
authors.lastname: Yeo
authors.forename: Alastair
authors.initials: A
authors.lastname: Compston
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Daly
authors.forename: Philip L
authors.initials: PL
authors.lastname: De Jager
authors.forename: Stacey
authors.initials: S
authors.lastname: Gabriel
authors.forename: David A
authors.initials: DA
authors.lastname: Hafler
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Ivinson
authors.forename: Eric S
authors.initials: ES
authors.lastname: Lander
authors.forename: John D
authors.initials: JD
authors.lastname: Rioux
authors.forename: Emily
authors.initials: E
authors.lastname: Walsh
authors.forename: Simon G
authors.initials: SG
authors.lastname: Gregory
authors.forename: Silke
authors.initials: S
authors.lastname: Schmidt
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Pericak-Vance
authors.forename: Lisa
authors.initials: L
authors.lastname: Barcellos
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hauser
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Oksenberg
authors.forename: Shannon J
authors.initials: SJ
authors.lastname: Kenealy
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Haines
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D005069:Evaluation Studies as Topic; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D009103:Multiple Sclerosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D006113:United Kingdom
chemical_list: D005819:Genetic Markers