Effect of ultrasound stimulation on metabolic modulation of pyruvic acid.
Pyruvic acid lay on the center position of Eremothecium ashbyii metabolic fluxes, during fermentation, a high performance liquid chromatography (HPLC) method is presented for the simultaneous determination of pyruvic acid in the Eremothecium ashbyii cell culture of both with ultrasound stimulation and the control. Dry weight and riboflavin are also mentored. The results of this study show that the ultrasound stimulation can give rise to pyruvic acid change, at the same time; change of pyruvic acid concentration is correlative to that of dry weight of mycelium and content of riboflavin.
Chuanyun
Dai
D
Key Laboratory of Biomechanics and Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China.
Bochu
Wang
W
Jian
Wang
W
Huan
Zhao
Z
D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects; D005285:Fermentation / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000737:chemistry / Q000528:radiation effects; D019289:Pyruvic Acid / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D012256:Riboflavin / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D014465:Ultrasonics
D003470:Culture Media; D019289:Pyruvic Acid; D012256:Riboflavin