title: Capillary electrophoresis-mass spectrometry for glycoscreening in biomedical research.
abstract: Application of capillary electrophoresis (CE) in combination with mass spectrometry (MS) and tandem MS to glycoscreening in biomedical projects is highlighted. In the first part recent CE-MS experiments by sheath liquid CE and multiple stage MS are reported. Neutral and negatively charged N-glycan mixtures from ribonuclease B and fetuin, high-mannose type N-glycoforms, oligosaccharides from lipopolysaccharides (LPS) of Haemophilus influenzae, polysaccharides of Pseudomonas aeruginosa and Staphylococcus aureus were analyzed. A particular emphasis is devoted to the applicability of novel off- and on-line CE-MS and tandem MS methods for screening of proteoglycan-derived oligosaccharides, glycosaminoglycans (GAGs), such as hyaluronates from Streptococcus agalactiae, chondroitin/dermatan sulfates (CS/DS) from bovine aorta and human skin fibroblast decorin, and heparin/heparan sulfate (HS) from porcine and bovine mucosa. The performance of CE-MS/MS for identification of glycoforms in glycopeptides and glycoproteins is illustrated by experiments performed on complex mixtures from urine of patients suffering from a hereditary N-acetylhexosaminidase deficiency (Schindler's disease) and urine of patients suffering from cancer cachexia. For determination of glycosylation patterns in glycoproteins like enzymes and antibodies by CE/MS, both CE-matrix assisted laser desorption/ionization (MALDI) and CE-electrospray ionization (ESI)-MS were functional. Finally, the potential of CE-ESI-MS strategy in glycolipid analysis is demonstrated for gangliosides from bovine brain for which particular CE buffer conditions are required.
authors.affiliation: Institute for Medical Physics and Biophysics, Biomedical Analysis Department, University of Münster, Münster, Germany.
authors.forename: Alina
authors.initials: A
authors.lastname: Zamfir
authors.affiliation: Institute for Medical Physics and Biophysics, Biomedical Analysis Department, University of Münster, Münster, Germany.
authors.forename: Jasna
authors.initials: J
authors.lastname: Peter-Katalinić
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000639:trends; D005732:Gangliosides / Q000032:analysis / Q000737:chemistry; D006020:Glycopeptides / Q000032:analysis / Q000737:chemistry; D006025:Glycosaminoglycans / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods* / Q000639:trends; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D011509:Proteoglycans / Q000032:analysis / Q000737:chemistry
chemical_list: D005732:Gangliosides; D006020:Glycopeptides; D006025:Glycosaminoglycans; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D011509:Proteoglycans