title: [Function of Tra2beta1 proteins in the splicing of neural-specific genes].
abstract: The in vitro studies have shown that Tra2beta1 proteins are important regulators in the alternative pre-mRNA splicing in mammalians. To date, the knowledge regarding the in vivo function of Tra2beta1 proteins, especially the function in regulating the splicing of neural-specific genes and the cell-type specificity of the functions is very limited. In the present study, the cell-type specific splicing of two neural-specific genes (GluR-B and SMN2), and the function of Tra2beta1 proteins in regulation of the splicing were studied in two types of cells (COS-1 and PFSK-1) using in vivo splicing models. The results showed that the splicing of GluR-B and SMN2 minigenes is cell-type specific, while Tra2beta1 proteins regulate the splicing of the minigenes in both cell lines in a similar pattern. It indicates that the Tra2beta1-regulated splicing of GluR-B and SMN2 minigenes regulated by Tra2beta1 is not cell-type specific.
authors.affiliation: Laboratory of Genomic Physiology, School of Life Sciences, Fudan University, Shanghai 200433.
authors.forename: Xian Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Wan Min
authors.initials: WM
authors.lastname: Lin
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D001932:Brain Neoplasms / Q000473:pathology; D019556:COS Cells / Q000378:metabolism; D045744:Cell Line, Tumor; D017362:Cyclic AMP Response Element-Binding Protein; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D018242:Neuroectodermal Tumors, Primitive / Q000473:pathology; D012322:RNA Precursors / Q000235:genetics; D016601:RNA-Binding Proteins; D018091:Receptors, AMPA / Q000096:biosynthesis / Q000235:genetics*; D055532:SMN Complex Proteins; D055540:Survival of Motor Neuron 2 Protein; D014162:Transfection
chemical_list: D015703:Antigens, CD; C497857:CD46 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D018091:Receptors, AMPA; D055532:SMN Complex Proteins; C526477:SMN2 protein, human; D055540:Survival of Motor Neuron 2 Protein; C115953:glutamate receptor type B