Gas chromatography-mass spectrometry determination of matrine in human plasma.
A method was developed for the quantification of matrine in human plasma using a liquid-liquid extraction procedure followed by gas-chromatography-mass spectrometry (GC/MS) analysis. Deuterated matrine, an internal standard of the analysis, was spiked into the plasma samples before extraction. Linear detection responses were obtained for matrine concentrations ranging from 10 to 500 ng/ml. The intra-day and inter-day precision ranged from 0.4 to 4.0% and 1.0-3.5%, respectively. The intra-day accuracy was between -7.3 and 4.5%. The limit of quantification for matrine was 23 ng/ml. The extraction efficiency averaged about 38%. The validated GC/MS method will be used to quantify matrine in human plasma samples collected in a clinical trial study.
Sit
Dan S T
DS
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6.
Gao
Guanghua
G
Law
Francis C P
FC
Li
Paul C H
PC
D000470:Alkaloids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011807:Quinolizines; D012015:Reference Standards; D015203:Reproducibility of Results; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines