Histopathological and immunohistochemical detection of protective effects of University of Wisconsin solution supplemented with iloprost on donor lung damage.
OBJECTIVE
Histopathological evaluation and immunohistochemical markers of surfactant B and CD34 were used to detect alveolar type II cell and pulmonary endothelial cell damage in order to assess the efficacy on donor lung protection of University of Wisconsin (UW) solution supplementation with iloprost.


METHODS
Twelve rats were divided into two groups: UW solution was used alone in group I, and UW iloprost solution in group II. Lung samples were taken at regular intervals for pathological examination to evaluate alveolar cell integrity with hematoxylin and eosin staining. Preservation, of alveolar type II cell and pulmonary endothelial cells was assessed using surfactant B and CD34 immunomarkers, respectively.


RESULTS
In both groups, alveolar integrity, surfactant, and CD34 revealed time-dependent, progressive damage, although this deterioration was less apparent among the iloprost-supplemented group. Alveolar integrity was better preserved at 4, 6, 8, 12, and 48 hours among group II rate. Surfactant staining showed significantly more deterioration at 12 and 24 hours in group I. Similarly, CD34 demonstrated significantly more injury at 6, 12, 24, and 48 hours in group I.


CONCLUSIONS
Although progressive lung tissue damage assessed by histopathological and immunohistochemical methods was observed in both groups, our findings suggest less deterioration in the iloprost-supplemented group.
Ustundag
N
N
Department of Pathology, Abant Izzet Baysal University Duzce Medical Faculty, Bolu, Turkey.
Bozkurt
A K
AK
Demirkaya
A
A
Koksal
C
C
Mayda
A S
AS
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D005978:Glutathione; D016285:Iloprost / Q000494:pharmacology*; D007150:Immunohistochemistry / Q000379:methods; D007328:Insulin; D008168:Lung / Q000187:drug effects / Q000473:pathology; D019188:Organ Preservation Solutions; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D011663:Pulmonary Surfactants / Q000032:analysis; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
D018952:Antigens, CD34; D007328:Insulin; D019188:Organ Preservation Solutions; D011663:Pulmonary Surfactants; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D016285:Iloprost; D000241:Adenosine; D011887:Raffinose