title: The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.
abstract: We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.
authors.forename: Edward
authors.initials: E
authors.lastname: Ryder
authors.forename: Fiona
authors.initials: F
authors.lastname: Blows
authors.forename: Michael
authors.initials: M
authors.lastname: Ashburner
authors.forename: Rosa
authors.initials: R
authors.lastname: Bautista-Llacer
authors.forename: Darin
authors.initials: D
authors.lastname: Coulson
authors.forename: Jenny
authors.initials: J
authors.lastname: Drummond
authors.forename: Jane
authors.initials: J
authors.lastname: Webster
authors.forename: David
authors.initials: D
authors.lastname: Gubb
authors.forename: Nicola
authors.initials: N
authors.lastname: Gunton
authors.forename: Glynnis
authors.initials: G
authors.lastname: Johnson
authors.forename: Cahir J
authors.initials: CJ
authors.lastname: O'Kane
authors.forename: David
authors.initials: D
authors.lastname: Huen
authors.forename: Punita
authors.initials: P
authors.lastname: Sharma
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Asztalos
authors.forename: Heiko
authors.initials: H
authors.lastname: Baisch
authors.forename: Janet
authors.initials: J
authors.lastname: Schulze
authors.forename: Maria
authors.initials: M
authors.lastname: Kube
authors.forename: Kathrin
authors.initials: K
authors.lastname: Kittlaus
authors.forename: Gunter
authors.initials: G
authors.lastname: Reuter
authors.forename: Peter
authors.initials: P
authors.lastname: Maroy
authors.forename: Janos
authors.initials: J
authors.lastname: Szidonya
authors.forename: Asa
authors.initials: A
authors.lastname: Rasmuson-Lestander
authors.forename: Karin
authors.initials: K
authors.lastname: Ekström
authors.forename: Barry
authors.initials: B
authors.lastname: Dickson
authors.forename: Christoph
authors.initials: C
authors.lastname: Hugentobler
authors.forename: Hugo
authors.initials: H
authors.lastname: Stocker
authors.forename: Ernst
authors.initials: E
authors.lastname: Hafen
authors.forename: Jean Antoine
authors.initials: JA
authors.lastname: Lepesant
authors.forename: Gert
authors.initials: G
authors.lastname: Pflugfelder
authors.forename: Martin
authors.initials: M
authors.lastname: Heisenberg
authors.forename: Bernard
authors.initials: B
authors.lastname: Mechler
authors.forename: Florenci
authors.initials: F
authors.lastname: Serras
authors.forename: Montserrat
authors.initials: M
authors.lastname: Corominas
authors.forename: Stephan
authors.initials: S
authors.lastname: Schneuwly
authors.forename: Thomas
authors.initials: T
authors.lastname: Preat
authors.forename: John
authors.initials: J
authors.lastname: Roote
authors.forename: Steven
authors.initials: S
authors.lastname: Russell
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional / Q000379:methods
chemical_list: D004251:DNA Transposable Elements