In vitro anti-coxsackievirus B(3) effect of ethyl acetate extract of Tian-hua-fen.
OBJECTIVE
To investigation the anti-coxsackievirus B(3) (CVB(3m)) effect of the ethyl acetate extract of Tian-hua-fen on HeLa cells infected with CVB(3m).


METHODS
HeLa cells were infected with CVB(3m) and the cytopathic effects (CPE) were observed through light microscope and crystal violet staining on 96-well plate and A(600) was detected using spectrophotometer. The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A(600).


RESULTS
The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration. Consistent results were got through these two methods. We also investigated the mechanism of its anti-CVB(3m) effect and the results indicated that the extract represented an inhibitory effect through all the processes of CVB(3m) attachment, entry, biosynthesis and assemble in cells.


CONCLUSIONS
The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significant protective effect on HeLa cells infected with CVB(3m) in a dose-dependent manner and this effect exists through the process of CVB(3m) attachment, entry, biosynthesis and assemble in cells, suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.
Li
Zhen-Hong
ZH
Department of Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China.
Nie
Bao-Ming
BM
Chen
Hong
H
Chen
Shu-Yun
SY
He
Ping
P
Lu
Yang
Y
Guo
Xiao-Kui
XK
Liu
Jing-Xing
JX
D000085:Acetates; D000998:Antiviral Agents / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D015978:Trichosanthin / Q000494:pharmacology*
D000085:Acetates; D000998:Antiviral Agents; D010936:Plant Extracts; D015978:Trichosanthin; C007650:ethyl acetate