Proteomic analysis of Trypanosoma cruzi developmental stages using isotope-coded affinity tag reagents.
Comparative proteome analysis of developmental stages of the human pathogen Trypanosoma cruzi was carried out by isotope-coded affinity tag technology (ICAT) associated with liquid cromatography-mass spectrometry peptide sequencing (LC-MS/MS). Protein extracts of the protozoan trypomastigote and amastigote stages were labeled with heavy (D8) and light (D0) ICAT reagents and subjected to cation exchange and avidin affinity chromatographies followed by LC-MS/MS analysis. High confidence sequence information and expression levels for 41 T. cruzi polypeptides, including metabolic enzymes, paraflagellar rod components, tubulins, and heat-shock proteins were reported. Twenty-nine proteins displayed similar levels of expression in both forms of the parasite, nine proteins presented higher levels in trypomastigotes, whereas three were more expressed in amastigotes.
Paba
Jaime
J
Brazilian Center for Protein Research, Department of Cell Biology, University of Brasilia, Brasilia 70910-900, DF, Brazil.
Ricart
Carlos A O
CA
Fontes
Wagner
W
Santana
Jaime M
JM
Teixeira
Antonio R L
AR
Marchese
Jason
J
Williamson
Brian
B
Hunt
Tony
T
Karger
Barry L
BL
Sousa
Marcelo V
MV
D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D007553:Isotope Labeling; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020543:Proteome; D015800:Protozoan Proteins / Q000032:analysis* / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000378:metabolism*
D020543:Proteome; D015800:Protozoan Proteins