Observation of electrostatically released DNA from gold electrodes with controlled threshold voltages.
DNA oligo-nucleotides, localized at Au metal electrodes in aqueous solution, are found to be released when applying a negative bias voltage to the electrode. The release was confirmed by monitoring the intensity of the fluorescence of cyanine dyes (Cy3) linked to the 5' end of the DNA. The threshold voltage of the release changes depending on the kind of linker added to the DNA 3'-terminal. The amount of released DNA depends on the duration of the voltage pulse. Using this technique, we can retain DNA at Au electrodes or Au needles, and release the desired amount of DNA at a precise location in a target. The results suggest that DNA injection into living cells is possible with this method.
Takeishi
Shunsaku
S
Fujitsu Laboratories Ltd., 10-1 Morinosato-Wakamiya, Atsugi 243-0197, Japan.
Rant
Ulrich
U
Fujiwara
Tsuyoshi
T
Buchholz
Karin
K
Usuki
Tatsuya
T
Arinaga
Kenji
K
Takemoto
Kazuya
K
Yamaguchi
Yoshitaka
Y
Tornow
Marc
M
Fujita
Shozo
S
Abstreiter
Gerhard
G
Yokoyama
Naoki
N
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055672:Static Electricity; D013997:Time Factors
D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D006046:Gold; D004247:DNA