Effects of citral on Aspergillus flavus spores by quasi-elastic light scattering and multiplex microanalysis techniques.
Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.
Luo
Man
M
College of Life Sciences, Wuhan University, China.
Jiang
Li-Ke
LK
Huang
Yao-Xiong
YX
Xiao
Ming
M
Li
Bo
B
Zou
Guo-Lin
GL
D000042:Absorption; D000080462:Acyclic Monoterpenes; D000935:Antifungal Agents / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004548:Elasticity; D008027:Light; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D039821:Monoterpenes / Q000494:pharmacology*; D025282:Mycelium / Q000648:ultrastructure; D010316:Particle Size; D010777:Photochemistry; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods; D013172:Spores, Fungal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000080462:Acyclic Monoterpenes; D000935:Antifungal Agents; D039821:Monoterpenes; C007076:citral