Generation of an infectious cDNA clone of an FMDV strain isolated from swine.
A full-length cDNA clone of a foot-and-mouth disease virus (FMDV) isolated from swine was assembled in, the plasmid vector pBluescript II SK+ downstream of a T7 promoter. RNA synthesized in vitro using T7 polymerase lead to the production of infectious particles upon transfection of BHK-21 cells, as shown by cytopathic effects. The rescued virus was also found to be highly pathogenic for mice by intradermal injection producing a fatal disease indistinguishable from that of wild-type virus. The availability of this cDNA clone will allow examination of the molecular mechanisms behind FMDV virulence and attenuation, which might in turn allow the production of second-generation, genetically engineered FMDV vaccines.
Liu
Guangqing
G
Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, 730046, PR China. liuguangqing@hotmail.com
Liu
Zaixin
Z
Xie
Qingge
Q
Chen
Yingli
Y
Bao
Huifang
H
Chang
Huiyun
H
Liu
Xiangtao
X
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D016679:Genome, Viral; D051379:Mice; D013552:Swine; D014774:Virulence
D018076:DNA, Complementary; D004279:DNA, Viral