Real-time visualization of the cytoskeleton and effector functions in T cells.
Advances in imaging technology have been essential to our understanding of T-cell activation and effector functions. Much of the progress stems from the use of fluorescent fusion proteins combined with high resolution imaging techniques, including confocal and multiphoton microscopy. However, these techniques have limitations, and other modes of imaging, including new developments on the horizon, might add promising new tools for the visualization of cytoskeleton-dependent processes in living cells.
Poenie
Martin
M
Department of Molecular Cell and Developmental Biology, University of Texas at Austin, 78712, USA. poenie@mail.utexas.edu
Kuhn
Jeffrey
J
Combs
Jeffrey
J
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D003599:Cytoskeleton / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008213:Lymphocyte Activation / Q000276:immunology*; D008853:Microscopy / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism