title: Maturation and integration of tissue-engineered cartilages within an in vitro defect repair model.
abstract: This study compared the behavior of four different engineered cartilages in a hybrid culture system. First, the growth and maturation of tissue-engineered cartilages in isolation were compared to those grown in an in vitro articular cartilage defect repair model. Tissue-engineered cartilages using fibrin, agarose, or poly(glycolic acid) scaffolds were implanted into annular explants of articular cartilage and cultured for 20 or 40 days. Native tissue had a substantial influence on the DNA, sulfated glycosaminoglycan, and hydroxyproline content of the engineered tissues, suggesting that the presence of living tissue in the culture significantly altered cell proliferation and matrix accumulation. Second, the adhesion strength of various engineered cartilages to native tissue was measured and compared with the biochemical content of the engineered tissues. All scaffold treatments adhered to the native cartilage, but there were statistically significant differences in adhesive strength between the different scaffolds. The adhesive strength of all engineered scaffolds was significantly lower than that of native tissue to itself. In the engineered tissues, neither failure stress nor energy to failure correlated with gross biochemical content, suggesting that adhesion between native and engineered tissues is not purely a function of gross matrix synthesis.
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0405, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hunter
authors.forename: Marc E
authors.initials: ME
authors.lastname: Levenston
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000293:injuries / Q000502:physiology / Q000637:transplantation; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology* / Q000637:transplantation; D005109:Extracellular Matrix / Q000000981:diagnostic imaging* / Q000502:physiology*; D013718:Tensile Strength / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D014463:Ultrasonography; D014945:Wound Healing / Q000502:physiology*