title: Changes of high-affinity choline transporter CHT1 mRNA expression during degeneration and regeneration of hypoglossal nerves in mice.
abstract: The high-affinity choline transporter CHT1 works for choline uptake in the presynaptic terminals of cholinergic neurons. We examined its expression in the hypoglossal nucleus after unilateral hypoglossal nerve transection in mice by fluorescent in situ hybridization. One week after axotomy, CHT1 mRNA expression was lost in all hypoglossal motoneurons in the lesioned side. Two weeks after axotomy, CHT1 mRNA started to be re-expressed in a few motoneurons that recovered connections to tongue muscles as revealed by retrograde labeling with Fast Blue. After 4 weeks, most of axotomized hypoglossal motoneurons were reconnected and re-expressed CHT1 mRNA as strongly as control neurons, and the regenerating cholinergic axons established mature neuromuscular junctions. These results suggest that the establishment of motor innervation is critical for CHT1 mRNA expression in hypoglossal neurons after axotomy.
authors.affiliation: Department of Pediatric Dentistry, Hokkaido University School of Dentistry, Sapporo 060-8586, Japan. shohei@den.hokudai.ac.jp
authors.forename: Shohei
authors.initials: S
authors.lastname: Oshima
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D019771:Axotomy; D007002:Hypoglossal Nerve / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D017403:In Situ Hybridization; D008297:Male; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000378:metabolism* / Q000502:physiology; D009416:Nerve Regeneration; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: C488255:CHT1 protein, mouse; D026901:Membrane Transport Proteins; D012333:RNA, Messenger