title: Isolation of nucleic acid binding proteins: an approach for isolation of cell surface, nucleic acid binding proteins.
abstract: An approach for isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e, and albumin.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Boris P
authors.initials: BP
authors.lastname: Chelobanov
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Laktionov
authors.forename: Maria V
authors.initials: MV
authors.lastname: Kharkova
authors.forename: Elena Y
authors.initials: EY
authors.lastname: Rykova
authors.forename: Valentin V
authors.initials: VV
authors.lastname: Vlassov
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006241:Haptens / Q000737:chemistry; D006801:Humans; D053550:Keratin-10; D053557:Keratin-2; D007633:Keratins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013058:Mass Spectrometry; D008970:Molecular Weight; D018307:Neoplasms, Squamous Cell / Q000473:pathology; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006241:Haptens; C507493:KRT10 protein, human; C089241:KRT2 protein, human; D053557:Keratin-2; D009838:Oligodeoxyribonucleotides; D012709:Serum Albumin; D053550:Keratin-10; D007633:Keratins