title: Effects of local administrations of tempol and diethyldithio-carbamic on peripheral nerve activity.
abstract: We have recently shown that systemic administration of a superoxide dismutase mimetic, tempol, resulted in decreases in mean arterial pressure and heart rate along with a reduction in renal sympathetic nerve activity (RSNA). It has also been shown that these parameters are significantly increased by systemic administration of a superoxide dismutase inhibitor, diethyldithio-carbamic (DETC), indicating a potential role of reactive oxygen species in the regulation of RSNA. In this study, we examined the effects of local administrations of 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol) and DETC on RSNA in anesthetized rats. Either tempol or DETC was directly administered onto the renal sympathetic nerves located between the electrode and ganglion. Local application of tempol (10 microL, 0.17 to 1.7 mol/L, n=6) resulted in dose-dependent decreases in integrated RSNA (by -81+/-6% at 1.7 mol/L) without alterations in mean arterial pressure and heart rate. In contrast, DETC (10 microL, 0.17 to 1.7 mol/L, n=6) increased RSNA dose-dependently. The responses of RSNA to tempol and DETC were significantly greater in spontaneously hypertensive rats than in normotensive rats (n=6, respectively). Local application of sodium nitroprusside (1 mmol/L) or N(G)-nitro-L-arginine methyl ester (0.11 mol/L) altered neither basal RSNA nor tempol-induced reductions in RSNA (n=6 and 5, respectively). A voltage-gated potassium channel blocker, 4-aminopyridine (0.1 mol/L), significantly decreased basal RSNA (by -81+/-1%) and completely prevented DETC-induced increases in RSNA (n=5). These results suggest that reactive oxygen species play a role in the regulation of peripheral sympathetic nerve activity, and that at least part of this mechanism is mediated through voltage-gated potassium channels.
authors.affiliation: Department of Pharmacology, Kagawa Medical University, Kita-Gun, Kagawa, Japan.
authors.forename: Takatomi
authors.initials: T
authors.lastname: Shokoji
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujisawa
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.forename: Matlubur
authors.initials: M
authors.lastname: Rahman
authors.forename: Hideyasu
authors.initials: H
authors.lastname: Kiyomoto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsubara
authors.forename: Kumiko
authors.initials: K
authors.lastname: Moriwaki
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Aki
authors.forename: Akira
authors.initials: A
authors.lastname: Miyatake
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kohno
authors.forename: Youichi
authors.initials: Y
authors.lastname: Abe
authors.forename: Akira
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004050:Ditiocarb / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects; D006973:Hypertension / Q000188:drug therapy / Q000503:physiopathology*; D007668:Kidney / Q000294:innervation*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D010525:Peripheral Nerves / Q000187:drug effects*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D018696:Neuroprotective Agents; D013113:Spin Labels; D009569:Nitric Oxide; D004050:Ditiocarb; C001803:tempol