Protochlorophyllide phototransformation in the bundle sheath cells of Zea mays.
The protochlorophyllide transformation process was investigated by using comparative analysis of 77 K fluorescence spectral changes occurring in isolated bundle sheath (BS) cells of etiolated Zea mays leaves after being exposed to a 200 ms saturating flash. Deconvolution analysis of the fluorescence spectra showed essential differences in the ratio of protochlorophyll(ides) and chlorophyll(ides) spectral forms indicating for BS cells to have a characteristic pathway of protochlorophyllide transformation. Bundle sheath cells showed a high ratio between non-photoactive protochlorophyll(ide)-F632 and photoactive protochlorophyllide-F655. In those cells, the 200 ms flash triggered a preferential formation of chlorophyll(ide)-F675 which remained stable in the dark for at least 90 min. Isolated BS cells showed an accumulation of chlorophyll(ide)-F675 resulting in the formation of inactive photosystem II. However for mesophyll cells of intact leaves, it was found to have a high ratio between photoactive and non-photoactive protochlorophyll(ide), showing the succession of chlorophyll(ide) forms usually known in C(3) plants. Protochlorophyllide phototransformation pathway in BS cells related to early stages of plastid differentiation triggered by light may indicate specific conditions for PSII assembly process leading to inactive PSII forms.
Marchand
Mathieu
M
Département de chimie et biochimie, TOXEN, Centre de recherche en toxicologie de l'environnement, Université du Québec à Montréal, Case postale 8888, Succursale Centre-Ville, Montréal, Québec, Canada H3C 3P8.
Dewez
David
D
Franck
Fabrice
F
Popovic
Radovan
R
D003080:Cold Temperature; D003624:Darkness; D001467:Hordeum / Q000378:metabolism / Q000528:radiation effects; D045332:Photosystem II Protein Complex; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects; D011521:Protochlorophyllide / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays; D003313:Zea mays / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
D045332:Photosystem II Protein Complex; D011521:Protochlorophyllide