title: Kruppel-like factor 6 germ-line mutations are infrequent in Finnish hereditary prostate cancer.
abstract: OBJECTIVE
  Recently, Kruppel-like factor 6 gene (KLF6) has been shown to be inactivated in up to 77% of prostate carcinomas. KLF6 has an important role in regulating cell growth and differentiation. The function and high mutation frequency in sporadic prostate carcinomas make KLF6 an attractive candidate for prostate cancer predisposition and, therefore, DNA samples from 69 Finnish prostate cancer families were analyzed for KLF6 mutations.
  
  
  METHODS
  DNA samples from 69 Finnish prostate cancer families were screened for mutations in the KLF6 gene using single-strand conformation polymorphism analysis and confirmatory sequencing.
  
  
  RESULTS
  In 8 (11.6%) families single-strand conformation polymorphism shifts were present. Sequencing revealed 6 201G>A (R201R) polymorphisms, as well as a -4C>A and a 956T>C alteration in the 5'- and 3'-untranslated regions, respectively. Nonsense or missense mutations were not found.
  
  
  CONCLUSIONS
  Our data suggest that KLF6 germ-line mutations are of marginal importance in prostate cancer predisposition in Finland.
authors.affiliation: Laboratory of Molecular Genetics, Centre for Laboratory Medicine, Tampere University Hospital, Finland. blpako@uta.fi
authors.forename: Pasi A
authors.initials: PA
authors.lastname: Koivisto
authors.forename: Eija-R
authors.initials: ER
authors.lastname: Hyytinen
authors.forename: Mika
authors.initials: M
authors.lastname: Matikainen
authors.forename: Teuvo L J
authors.initials: TL
authors.lastname: Tammela
authors.forename: Tarja
authors.initials: T
authors.lastname: Ikonen
authors.forename: Johanna
authors.initials: J
authors.lastname: Schleutker
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D005387:Finland / Q000453:epidemiology; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics; D018095:Germ-Line Mutation; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D008297:Male; D008875:Middle Aged; D018807:Polymorphism, Single-Stranded Conformational; D011471:Prostatic Neoplasms / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D016036:Seroepidemiologic Studies; D015534:Trans-Activators / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators