Storage of platelets in additive solution for up to 12 days with maintenance of good in-vitro quality.
BACKGROUND
Storage of PLT concentrates (PCs) may be extended beyond 5 days, provided in-vitro and in-vivo variables allow longer storage and bacterial screening is performed. The aim of this study was to examine in-vitro storage characteristics of PCs in various storage solutions: plasma only, or mixtures of plasma with PAS-II, PAS-III, PAS-IIIM, and Composol.


METHODS
PCs from five pooled buffy-coats and WBCs reduced by filtration were stored in 1.3-L butyryl-tri-hexyl-citrate-plasticised PVC containers. First, a paired comparison was made between PAS-II and Composol, with 35-percent final plasma concentration (n = 10). Then, plasma, PAS-III with 30-percent plasma, PAS-IIIM with 20- and 30-percent plasma, and Composol with 20 and 30-percent final plasma concentration were compared (n = 5 pairs). Finally, 50 PCs in Composol with 35-percent final plasma concentration were studied.


RESULTS
PCs in PAS-II or Composol had a mean +/- SD pH of 6.95 +/- 0.09 and 6.96 +/- 0.08 at Day 12, respectively. For PCs in PAS-IIIM and Composol with 30-percent final plasma concentration, pH on Day 7 was 7.00 +/- 0.02 and 6.83 +/- 0.05. With 20-percent final plasma concentration, pH was 6.98 +/- 0.02 and 6.81 +/- 0.03 for PAS-IIIM and Composol, respectively. PCs in PAS-III with 30-percent plasma had a pH on Day 7 of 6.87 +/- 0.03, whereas PCs in 100-percent plasma had a pH of 7.05 +/- 0.03. PCs in Composol with 35-percent plasma maintained pH greater than 6.8 in 48 of 50 of the units (96%), averaging 7.00 +/- 0.10 on Day 8.


CONCLUSIONS
In-vitro quality of PCs in AS with at least 35-percent plasma can be maintained for 7 to 12 days after collection.
van der Meer
Pieter F
PF
Sanquin Blood Bank North-west Region, Amsterdam, the Netherlands. p.vandermeer@sanquin.nl
Pietersz
Ruby N I
RN
Reesink
Henk W
HW
D000085:Acetates / Q000494:pharmacology*; D001792:Blood Platelets; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D000085:Acetates; D002951:Citrates; C436265:PAS-2 salt solution; D011310:Preservatives, Pharmaceutical; D012965:Sodium Chloride