title: DpsA protects the human pathogen Burkholderia pseudomallei against organic hydroperoxide.
abstract: The human pathogen, Burkholderia pseudomalle, is able to survive and multiply in hostile environments such as within macrophages. In an attempt to understand its strategy to cope with oxidative stress, the physiological role and gene regulation of a nonspecific DNA-binding protein (DpsA) was investigated. Expression of dpsA increases in response to oxidative stress through increased transcription from the upstream katG (catalase-peroxidase) promoter, which is OxyR dependent. dpsA is also transcribed from its own promoter, which is activated by osmotic stress in an OxyR-independent manner. DpsA-deficient mutants are hypersensitive to tert-butyl hydroperoxide, while overexpression of DpsA leads to increased resistance to organic oxidants. B. pseudomallei DpsA can also protect Escherichia coli against organic hydroperoxide toxicity. The mechanism of DpsA-mediated resistance to organic hydroperoxides was shown to differ from that of alkyl hydroperoxide reductase.
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, 10210, Bangkok, Thailand. suvit@tubtim.cri.or.th
authors.forename: Suvit
authors.initials: S
authors.lastname: Loprasert
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Whangsuk
authors.forename: Ratiboot
authors.initials: R
authors.lastname: Sallabhan
authors.forename: Skorn
authors.initials: S
authors.lastname: Mongkolsuk
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D016957:Burkholderia pseudomallei / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D016877:Oxidants; D006861:Hydrogen Peroxide