title: [Immunological reaction between the peptides from S1 domain of SARS coronavirus S-protein and the serum from SARS patients].
abstract: OBJECTIVE
  To examine the immunological reactions between the peptides of SARS coronavirus (SARS-Cov) S-protein and the serum of SARS patients for identifying the SARS-Cov epitope.
  
  
  METHODS
  The peptides from S1 domain of SARS-Cov S-protein were synthesized by peptide synthesizer, and the immunological reaction of the peptides with the serum of SARS patients were examined by means of ELISA.
  
  
  RESULTS
  The optical density value of the immunological reaction of synthesized 8 peptides with SARS patient serum was 1.5-2.6 times higher than that with normal serum.
  
  
  CONCLUSIONS
  The 8 peptides from S1 domain of S protein appear to have low immunogenicity to the serum of SARS patients, indicating that these peptides may not be the epitope of the SARS-Cov.
authors.affiliation: Institute of Pharmaceutics Science, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jydyys@fimmu.com
authors.forename: Jia-jie
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Shao-yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Jin-lin
authors.initials: JL
authors.lastname: Hou
authors.forename: Zhan-hui
authors.initials: ZH
authors.lastname: Wang
authors.forename: Shi-bo
authors.initials: SB
authors.lastname: Jiang
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000939:Epitopes; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000097:blood*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000939:Epitopes; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus