The use of comet assay data with a simple reaction mechanism to evaluate the relative effectiveness of free radical scavenging by quercetin, epigallocatechin gallate and N-acetylcysteine in UV-irradiated MRC5 lung fibroblasts.
Comet assay data (tail DNA %) have been gathered for the concentration dependent role of three antioxidants (AOs); quercetin (Q), epigallocatechin gallate (EGCG) and N-acetylcysteine (NAC) in reducing UV-induced damage to DNA in normal fetal lung fibroblasts (MRC5). All three compounds demonstrate a concentration dependent reduction maximum with a pro-oxidant effect at higher (though not cytotoxic) concentrations. Manipulation of a simple 4-step reaction mechanism for free radical (FR) scavenging by AOs produced rate constant ratios which allowed the relative effectiveness (Q > EGCG > NAC) of the AOs to be evaluated.
Salter
Leo
L
Cornwall Dermatology Research Project, Public Health Laboratories, Polgooth Ward, Royal Cornwall Hospital Treliske, Truro Cornwall TR1 3LJ, UK.
Clifford
Tim
T
Morley
Nick
N
Gould
David
D
Campbell
Sandra
S
Curnow
Alison
A
D000111:Acetylcysteine / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D011794:Quercetin / Q000494:pharmacology*; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; D002392:Catechin; D004247:DNA; D011794:Quercetin; C045651:epigallocatechin gallate; D000111:Acetylcysteine