title: Control of superoxide production in mitochondria from maize mesocotyls.
abstract: To understand the biochemical events that control the generation of superoxide, the effect of inhibiting the respiratory complexes III and IV (C-III and C-IV) and alternative oxidase (AOX) on the rate of superoxide production was analyzed in mitochondria from maize seedlings. To increase superoxide production, it was required to inhibit C-III or C-IV by at least 30% or 50%, respectively. Below this inhibition threshold, AOX exerted the highest degree of control on superoxide production, whereas above it, the highest degree of control was exerted by C-IV. The contribution of C-III to control superoxide production became significant when AOX activity was modulated.
authors.affiliation: Departamento de Bioquímica, Facultad de Química, UNAM, 04510 Mexico D.F. camacho@servidor.unam.mx
authors.forename: Adriana
authors.initials: A
authors.lastname: Camacho
authors.forename: Rafael
authors.initials: R
authors.lastname: Moreno-Sanchez
authors.forename: Irma
authors.initials: I
authors.lastname: Bernal-Lugo
mesh_terms: D004305:Dose-Response Relationship, Drug; D004579:Electron Transport; D014450:Electron Transport Complex III; D003576:Electron Transport Complex IV / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010940:Plant Proteins; D018514:Plant Structures / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013696:Temperature; D003313:Zea mays / Q000378:metabolism*; D000099188:Alternative Oxidase
chemical_list: D014450:Electron Transport Complex III; D003576:Electron Transport Complex IV; D004791:Enzyme Inhibitors; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010100:Oxygen; D010940:Plant Proteins; D013481:Superoxides; D000099188:Alternative Oxidase