title: Plasticity of mouse renal collecting duct in response to potassium depletion.
abstract: Plasticity of mouse renal collecting duct in response to potassium depletion.--Renal collecting ducts are the main sites for regulation of whole body potassium balance. Changes in dietary intake of potassium induce pleiotropic adaptations of collecting duct cells, which include alterations of ion and water transport properties along with an hypertrophic response. To study the pleiotropic adaptation of the outer medullary collecting duct (OMCD) to dietary potassium depletion, we combined functional studies of renal function (ion, water, and acid/base handling), analysis of OMCD hypertrophy (electron microscopy) and hyperplasia (PCNA labeling), and large scale analysis of gene expression (transcriptome analysis). The transcriptome of OMCD was compared in mice fed either a normal or a potassium-depleted diet for 3 days using serial analysis of gene expression (SAGE) adapted for downsized extracts. SAGE is based on the generation of transcript-specific tag libraries. Approximately 20,000 tags corresponding to 10,000 different molecular species were sequenced in each library. Among the 186 tags differentially expressed (P < 0.05) between the two libraries, 120 were overexpressed and 66 were downregulated. The SAGE expression profile obtained in the control library was representative of different functional classes of proteins and of the two cell types (principal and alpha-intercalated cells) constituting the OMCD. Combined with gene expression analysis, results of functional and morphological studies allowed us to identify candidate genes for distinct physiological processes modified by potassium depletion: sodium, potassium, and water handling, hyperplasia and hypertrophy. Finally, comparison of mouse and human OMCD transcriptomes allowed us to address the question of the relevance of the mouse as a model for human physiology and pathophysiology.
authors.affiliation: Laboratoire de Physiologie et Génomique Rénales, Unité Mixte de Recherche Centre National de la Recherche Scientifique/UPMC 7134, Paris, France.
authors.forename: Lydie
authors.initials: L
authors.lastname: Cheval
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Duong Van Huyen
authors.forename: Patrick
authors.initials: P
authors.lastname: Bruneval
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Verbavatz
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Elalouf
authors.forename: Alain
authors.initials: A
authors.lastname: Doucet
mesh_terms: D000136:Acid-Base Equilibrium / Q000235:genetics / Q000502:physiology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001835:Body Weight; D020869:Gene Expression Profiling; D006801:Humans; D006965:Hyperplasia / Q000235:genetics / Q000473:pathology; D006984:Hypertrophy / Q000235:genetics / Q000473:pathology; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D011191:Potassium Deficiency / Q000097:blood / Q000235:genetics* / Q000503:physiopathology* / Q000652:urine; D017419:Potassium, Dietary / Q000008:administration & dosage / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D017419:Potassium, Dietary; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger