Comparison of viability of B16 and Cl S91 cells in three cytotoxicity tests: cells counting, MTT and flow cytometry after cytostatic drug treatment.
In this study, results of three tests assessing viability of B16 and Cl S91 mouse melanoma cells after exposure to cytostatic drugs were compared: alive cell counting test after fixing with ethyl alcohol, MTT test and annexin V-FITC flow cytometry test. On the grounds of the obtained results the last mentioned method was recognized to be the most accurate and the least faulty. It was stated that mouse melanoma B16 cells are more sensitive to actinomycin D. cytosine arabinoside, cisplatin and dacarbazine than mouse melanoma Cl S91 cells. Mouse melanoma Cl S91 cells are more sensitive to vincristine than B16 cells.
Olszewska-Słonina
Dorota
D
Department of Medical Biology, 2 Department and Clinic of Orthopedics and Traumathology, Rydygier Medical University, 24 Karlowicza Str., 85-092 Bydgoszcz, Poland.
Drewa
Tomasz
T
Musiałkiewicz
Dariusz
D
Olszewski
Krzysztof
K
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D004353:Drug Evaluation, Preclinical; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D011419:Propidium; D013778:Tetrazolium Salts; D013844:Thiazoles
D017304:Annexin A5; D000970:Antineoplastic Agents; D004396:Coloring Agents; D005456:Fluorescent Dyes; D013778:Tetrazolium Salts; D013844:Thiazoles; D011419:Propidium; C022616:thiazolyl blue; D016650:Fluorescein-5-isothiocyanate