title: Targeted generation of 16 sequence-tagged sites for bovine chromosome region 5q21-q25 by microdissection.
abstract: The cattle chromosome region BTA5q21-q25 is associated with immune defense and is linked with several economically important traits for growth, ovulation, twinning and milk yield. Chromosome microdissection of the region was performed to generate fragment-specific DNA libraries and to provide new bovine-specific DNA sequences as starting material for physical fine-mapping approaches or linkage studies. A first library screening resulted in the identification of 16 region-specific sequence-tagged sites (STS). Fifteen STS represent new unique cattle DNA sequences, of which one identifies a new microsatellite. Homology with the gene CDK2 was found for the remaining STS.
authors.affiliation: Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
authors.forename: Karin
authors.initials: K
authors.lastname: Rottengatter
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Weikard
authors.forename: Rolf
authors.initials: R
authors.lastname: Horstmann
authors.forename: Annette
authors.initials: A
authors.lastname: Gehlhaus
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Brunner
authors.forename: Olivier
authors.initials: O
authors.lastname: Hanotte
authors.forename: Manfred
authors.initials: M
authors.lastname: Schwerin
mesh_terms: D000818:Animals; D001483:Base Sequence; D042846:CDC2-CDC28 Kinases / Q000235:genetics; D002417:Cattle; D002875:Chromosomes / Q000235:genetics*; D051357:Cyclin-Dependent Kinase 2; D005260:Female; D015723:Gene Library; D007109:Immunity / Q000235:genetics; D007774:Lactation / Q000235:genetics; D008297:Male; D042282:Microdissection; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010060:Ovulation / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D038522:Twinning, Monozygotic / Q000276:immunology
chemical_list: D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2