title: Coumarin content and physicochemical profile of Mikania laevigata extracts.
abstract: The 'guaco' lianous herb Mikania laevigata, which is widespread in Southern Brazil, is traditionally used to treat bronchitis, asthma and cough. This work investigates the influence of the extraction method, solvent:drug ratio, ethanol proportion, harvest season (summer or winter) and solvent heating on the physicochemical profile of the extracts (dry weight, density, pH) and the coumarin (1,2-benzopyrone) content determined by LC. Among the results obtained, it is observed that higher ethanol content increases the amount of coumarin in the extract. Leaves harvested in summer also produce an extract with a high coumarin yield. The most efficient method of extraction is percolation, independent of the solvent used.
authors.affiliation: Universidade do Vale do Itajaí (UNIVALI), Centro de Ciências da Sadde (CCS), Curso de Farmácia, CP 360, 88303-202, Itajaí, Santa Catarina (SC), Brazil. maique@ccs.univali.br
authors.forename: Maique W
authors.initials: MW
authors.lastname: Biavatti
authors.forename: Cesar A
authors.initials: CA
authors.lastname: Koerich
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Henck
authors.forename: Enderson
authors.initials: E
authors.lastname: Zucatelli
authors.forename: Fernanda H
authors.initials: FH
authors.lastname: Martineli
authors.forename: Tania B
authors.initials: TB
authors.lastname: Bresolin
authors.forename: Silvana N
authors.initials: SN
authors.lastname: Leite
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D035742:Mikania / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C030123:coumarin