Arterial chemoreceptors in the superior laryngeal nerve of the rat.
Paraganglia resembling the carotid body have been described in the superior laryngeal nerve (SLN) of the rat and the aim of the present study was to determine if this tissue is chemosensitive. We developed a novel isolated SLN preparation superfused with HEPES-buffered Tyrode solution at 35 degrees C in vitro. A glass suction microelectrode was used to record the electrical activity of single SLN units and a micropipette was used to pressure-eject small volumes of sodium cyanide (NaCN; 250-500 ng in 5 microl) near glomus tissue located at the main bifurcation of the SLN. The duration of the NaCN response and the number of spikes evoked after application of NaCN were compared in normoxia and hyperoxia (PO2 > 300 mmHg). Hyperoxia significantly reduced the duration and spike number of the NaCN response and a negative linear correlation existed between PO2 and response duration. In addition, hypoxia (PO2 < 60 mmHg) triggered SLN firing. Therefore, we can conclude that the paraganglia of the SLN are not only morphologically similar to the carotid body but are also excited by similar stimuli.
O'Leary
Deirdre M
DM
Department of Human Anatomy and Physiology, Division of Physiology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Earlsfort Terrace, Dublin 2, Ireland.
Murphy
Andrew
A
Pickering
Mark
M
Jones
James F X
JF
D000818:Animals; D001158:Arteries / Q000294:innervation*; D002628:Chemoreceptor Cells / Q000502:physiology*; D005071:Evoked Potentials / Q000502:physiology*; D017950:Ganglia, Sensory / Q000166:cytology / Q000502:physiology*; D018496:Hyperoxia / Q000139:chemically induced; D066298:In Vitro Techniques; D007823:Laryngeal Nerves / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012966:Sodium Cyanide
D012966:Sodium Cyanide