Glycolipid class profiling by packed-column subcritical fluid chromatography.
The potential of packed-column subcritical fluid chromatography (SubFC) for the separation of lipid classes has been assessed in this study. Three polar stationary phases were checked: silica, diol, and poly(vinyl alcohol). Carbon dioxide (CO2) with methanol as modifier was used as mobile phase and detection performed by evaporative light scattering detection. The influence of methanol content, temperature, and pressure on the chromatographic behavior of sphingolipids and glycolipids were investigated. A complete separation of lipid classes from a crude wheat lipid extract was achieved using a modifier gradient from 10 to 40% methanol in carbon dioxide. Solute selectivity was improved using coupled silica and diol columns in series. Because the variation of eluotropic strength depending on the fluid density changes, a normalized separation factor product (NSP) was used to select the nature, the number and the order of the columns to reach the optimum glycolipid separation.
Deschamps
Frantz S
FS
Laboratoire de Chimie Analytique, Faculté de Pharmacie, 1 rue Jean-Baptiste Clement, 92296 Châtenay-Malabry Cedex, France.
Lesellier
Eric
E
Bleton
Jean
J
Baillet
Arlette
A
Tchapla
Alain
A
Chaminade
Pierre
P
D002853:Chromatography, Liquid / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry* / Q000145:classification; D011312:Pressure; D013696:Temperature
D006017:Glycolipids