title: Relationship between gyrA mutations and quinolone resistance in Flavobacterium psychrophilum isolates.
abstract: Flavobacterium psychrophilum is the causative agent of the fish diseases called bacterial cold-water disease and rainbow trout fry syndrome. It has been reported that some isolates of F. psychrophilum are resistant to quinolones; however, the mechanism of this quinolone resistance has been unexplained. In this study, we examined the quinolone susceptibility patterns of 27 F. psychrophilum strains isolated in Japan and the United States. Out of 27 isolates, 14 were resistant to both nalidixic acid (NA) and oxolinic acid (OXA), and the others were susceptible to NA and OXA. When amino acid sequences deduced from gyrA nucleotide sequences of all isolates tested were analyzed, two amino acid substitutions (a threonine residue replaced by an alanine or isoleucine residue in position 83 of GyrA [Escherichia coli numbering] and an aspartic acid residue replaced by a tyrosine residue in position 87) were observed in the 14 quinolone-resistant isolates. These results strongly suggest that, as in other gram-negative bacteria, DNA gyrase is an important target for quinolones in F. psychrophilum.
authors.affiliation: Molecular Biology Division, National Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan. sizumi@affrc.go.jp or s-izumi@aa.e-mansion.com
authors.forename: Shotaro
authors.initials: S
authors.lastname: Izumi
authors.forename: Futoshi
authors.initials: F
authors.lastname: Aranishi
mesh_terms: D000595:Amino Acid Sequence; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005417:Flavobacterium / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D010093:Oxolinic Acid / Q000494:pharmacology*; D016133:Polymerase Chain Reaction
chemical_list: D009268:Nalidixic Acid; D027081:DNA Gyrase; D010093:Oxolinic Acid