title: Regulation of sister chromatid cohesion between chromosome arms.
abstract: Sister chromatid separation in anaphase depends on the removal of cohesin complexes from chromosomes. In vertebrates, the bulk of cohesin is already removed from chromosome arms during prophase and prometaphase, whereas cohesin remains at centromeres until metaphase, when cohesin is cleaved by the protease separase. In unperturbed mitoses, arm cohesion nevertheless persists throughout metaphase and is principally sufficient to maintain sister chromatid cohesion. How arm cohesion is maintained until metaphase is unknown. Here we show that small amounts of cohesin can be detected in the interchromatid region of metaphase chromosome arms. If prometaphase is prolonged by treatment of cells with microtubule poisons, these cohesin complexes dissociate from chromosome arms, and arm cohesion is dissolved. If cohesin dissociation in prometaphase-arrested cells is prevented by depletion of Plk1 or inhibition of Aurora B, arm cohesion is maintained. These observations imply that, in unperturbed mitoses, small amounts of cohesin maintain arm cohesion until metaphase. When cells lacking Plk1 and Aurora B activity enter anaphase, chromatids lose cohesin. This loss is prevented by proteasome inhibitors, implying that it depends on separase activation. Separase may therefore be able to cleave cohesin at centromeres and on chromosome arms.
authors.affiliation: Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Giménez-Abián
authors.forename: Izabela
authors.initials: I
authors.lastname: Sumara
authors.forename: Toru
authors.initials: T
authors.lastname: Hirota
authors.forename: Silke
authors.initials: S
authors.lastname: Hauf
authors.forename: Daniel
authors.initials: D
authors.lastname: Gerlich
authors.forename: Consuelo
authors.initials: C
authors.lastname: de la Torre
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.forename: Jan-Michael
authors.initials: JM
authors.lastname: Peters
mesh_terms: D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000378:metabolism; D002478:Cells, Cultured / Q000166:cytology; D002842:Chromatids / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone; D002877:Chromosomes, Human / Q000502:physiology*; D010450:Endopeptidases / Q000378:metabolism; D005656:Fungal Proteins; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D015151:Immunoblotting; D008677:Metaphase / Q000502:physiology; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000502:physiology*; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism* / Q000502:physiology; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034622:RNA Interference; D051381:Rats; D064247:Separase; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
chemical_list: D064107:Aurora Kinase B; D064127:Aurora Kinases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D010450:Endopeptidases; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D064247:Separase; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C576921:AURKB protein, human; C576925:Aurkb protein, rat; C577987:ESPL1 protein, human