title: [The spread of the wild Poliovirus in the rural environment, the case of the Adzopé health district, Côte d'Ivoire].
abstract: Wild Poliovirus spreading in rural environment in Adzopé, Côte d'Ivoire In order to determine the level of wild Poliovirus spreading among rural children in an endemic poliomyelitis country 469 stools samples, from children aged between three weeks and twelve years old were processed according to WHO procedures for transportation, conservation, isolation and identification of Poliovirus. Intratypic differenciation was performed by an antigenic method using monoclonal antibodies and a genomic RFLP (Restriction Fragment Length Polymorphism). 50 Poliovirus strains (10.7%) were isolated and analyzed: 15 vaccine-like Poliovirus type 1 (30%), 30 vaccine-like Poliovirus type 2 (60%), 4 vaccine-like Poliovirus type 3 (8%) and one wild Poliovirus type 3 (2%). As expected, in the major cases the duration of post-vaccinal viral excretion did not exceed two months. However, in 14% of cases, it varied between 3 and 9 months after the third OPV dose. This long excretion could be due to an inefficient local intestinal immunity or no local immunity at all, in spite of the three OPV doses. These results argue in favor of an increase of the number of OPV doses in such endemic zones. Moreover, OPV strains are well-known to revert to pathogenicity in vaccinees, therefore, the long term excretion of pathogenic OPV derived strains by a certain amount of vaccinees needs to be considered quite seriously.
authors.affiliation: Laboratoire des entérovirus, Institut Pasteur de Côte d'Ivoire, BP 490 Abidjan 01, Côte d'Ivoire. polioci@globeaccess.net
authors.forename: C G
authors.initials: CG
authors.lastname: Akoua-Koffi
authors.forename: G
authors.initials: G
authors.lastname: Nekouressi
authors.forename: L
authors.initials: L
authors.lastname: Tieoulou
authors.forename: S
authors.initials: S
authors.lastname: Guillot
authors.forename: H
authors.initials: H
authors.lastname: Faye-Kette
authors.forename: A
authors.initials: A
authors.lastname: Ehouman
mesh_terms: D000914:Antibodies, Viral; D002648:Child; D002675:Child, Preschool; D007560:Cote d'Ivoire / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D019353:Endemic Diseases / Q000517:prevention & control / Q000706:statistics & numerical data*; D005243:Feces / Q000821:virology; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D017589:Immunization Programs; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology; D011051:Poliomyelitis / Q000453:epidemiology* / Q000517:prevention & control / Q000635:transmission / Q000821:virology*; D017955:Poliovirus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D011159:Population Surveillance; D012423:Rural Health / Q000706:statistics & numerical data*; D012703:Serotyping; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D013997:Time Factors; D017201:Virus Shedding / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral