A novel HLA-DRB1*13 allele (DRB1*1357) identified by polymerase chain reaction with sequence-specific primers and direct sequencing.
We describe the identification of a new DRB1*13 allele, DRB1*1357*, found in two Austrian Caucasian individuals. The novel allele was initially suspected because analysis with sequence-specific primers resulted in an unusual pattern of amplification. Thereafter, exon 2 was further characterized by sequence-based typing. The nucleotide sequence of DRB1*1357 is identical to DRB1*1319 except for a single substitution in codon 47 (TAC to TTC) leading to a change from phenylalanine to tyrosine.
Danzer
M
M
Red Cross Transfusion Service of Upper Austria, Linz, Austria.
Leitner
D
D
Gangl
E
E
Faé
I
I
Fischer
G F
GF
Gabriel
C
C
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D044465:White People
D017931:DNA Primers; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains