[Case-only study on the relationship between genetic polymorphisms in toxicant metabolizing enzymes and risk of occupational chronic benzene poisoning].
OBJECTIVE
To explore the effects of interaction between environmental exposure factors and genetic polymorphism in toxicant metabolizing enzymes on risk of occupational chronic benzene poisoning.


METHODS
One hundred and fifty-two cases of chronic benzene poisoning were analyzed for the risk by case-only study.


RESULTS
The frequency of non-null GSTT1 gene in benzene poisoning workers with moderate benzene exposure level was higher than that in cases with lower benzene exposure (68.63% vs 38.00%, OR(adj) = 4.32, 95% CI 1.75 - 10.66, P = 0.002). The frequency of NQO1 C.609T/T gene in alcohol drinking group was higher than that in non-drinking group (61.11% vs 20.00%, OR(adj) = 8.03, 95% CI 2.28 - 28.25, P = 0.001), moreover, it was higher in workers with smoking and drinking than that in the rest group, and in drinking x exposure level workers than that in non-drinking x exposure level workers (85.71% vs 22.76%, OR(adj) = 18.62, 95% CI 2.01 - 172.72, P = 0.01 and 61.11% vs 20.00%, OR(adj) = 3.18, 95% CI 1.55 - 6.52, P = 0.002 respectively). The frequency of non-null GSTM1 gene was also higher in drinking x exposure level workers than that in non-drinking x exposure level workers (66.67% vs 47.06%, OR(adj) = 1.99, 95% CI 1.05 - 3.76, P = 0.036).


CONCLUSIONS
There is interaction between the polymorphism of GSTT1 gene and moderate benzene exposure level; non-null GSTM1 gene and drinking x exposure level increase the risk of occupational chronic benzene poisoning; polymorphism of NQO1 gene C.609 also interacts with drinking, while polymorphism of NQO1 gene and drinking x smoking may further increase the risk of occupational chronic benzene poisoning.
Zhang
Zhong-bin
ZB
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Wan
Jun-xiang
JX
Xia
Zhao-lin
ZL
D000328:Adult; D001554:Benzene / Q000378:metabolism / Q000506:poisoning*; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D009784:Occupational Diseases / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D001554:Benzene; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human