title: Disruption of the RAD51 gene sensitizes S. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide.
abstract: The RAD51 gene was disrupted in three different parental wild-type strains to yield three rad51 null strains with different genetic background. The rad51 mutation sensitizes yeast cells to the toxic and mutagenic effects of H2O2, suggesting that Rad51-mediated repair, similarly to that of RecA-mediated, is relevant to the repair of oxidative damage in S. cerevisiae. Moreover, pulsed-field gel electrophoresis analysis demonstrated that increased sensitivity of the rad51 mutant to H2O2 is accompanied by its decreased ability to repair double-strand breaks induced by this agent. Our results show that ScRad51 protects yeast cells from H2O2-induced DNA double-strand breakage.
authors.affiliation: Laboratory of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia.
authors.forename: Z
authors.initials: Z
authors.lastname: Dudásová
authors.forename: A
authors.initials: A
authors.lastname: Dudás
authors.forename: A
authors.initials: A
authors.lastname: Alemayehu
authors.forename: D
authors.initials: D
authors.lastname: Vlasáková
authors.forename: E
authors.initials: E
authors.lastname: Marková
authors.forename: M
authors.initials: M
authors.lastname: Chovanec
authors.forename: V
authors.initials: V
authors.lastname: Vlcková
authors.forename: J
authors.initials: J
authors.lastname: Brozmanová
mesh_terms: D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000502:physiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051135:Rad51 Recombinase; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase