Fabrication of alternating polycation and albumin multilayer coating onto stainless steel by electrostatic layer-by-layer adsorption.
Multilayer films consisting of polyethylenimine (PEI) and albumin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly of the PEI and albumin. The process of electrostatic self-assembly of PEI/albumin was monitored by125I radiolabeling, electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM). The EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. 125I radiolabeling experiments indicated that less than 10% albumin was eluted by PBS in 45 days. Static platelet adhesion experiments indicated that the PEI/albumin deposited on stainless steel could resist platelet adhesion effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices.
Ji
Jian
J
Department of Polymer Science, Zhejiang University, Hangzhou 310027, China. jianji@mail.hz.zj.cn
Tan
Qinggang
Q
Fan
De-Zeng
DZ
Sun
Fu-Yu
FY
Barbosa
M A
MA
Shen
Jiacong
J
D000327:Adsorption; D000418:Albumins / Q000737:chemistry* / Q000378:metabolism; D017097:Electric Impedance; D004583:Electrons; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D010973:Platelet Adhesiveness; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry; D013057:Spectrum Analysis; D013193:Stainless Steel / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
D000418:Albumins; D008051:Lipid Bilayers; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D013193:Stainless Steel