title: Expression profiles of tyrosine kinases in cultured follicular papilla cells versus dermal fibroblasts.
abstract: Tyrosine kinases play crucial roles in cell differentiation and proliferation. Using degenerative primed PCR followed by differential display, we analyzed the tyrosine kinase expression profiles of cultured rat follicular papilla (FP) cells versus dermal fibroblasts. We showed that c-met, cdc2, and tec were preferentially expressed in cultured FP cells, whereas alpha-platelet-derived growth factor receptor (alpha-PDGFR) was preferentially expressed in cultured fibroblasts. The cell type specificity of these tyrosine kinases was confirmed by semi-quantitative RT-PCR using both rat and human cultured cells. Consistent with these results, hepatocyte growth factor preferentially stimulated the growth of rat FP cells, whereas PDGF-AA preferentially stimulated rat fibroblasts. High concentrations of some these kinases are also found in the follicular matrix keratinocytes as revealed by in situ hybridization. The expression of specific tyrosine kinases in FP and matrix cells may play roles in regulating hair growth and cycling.
authors.affiliation: Epithelial Biology Unit, Ronald O. Perelman Department of Dermatology, NYU Cancer Institute, New York University School of Medicine, New York 10016, USA.
authors.forename: Dawen
authors.initials: D
authors.lastname: Yu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Cao
authors.forename: Zhijun
authors.initials: Z
authors.lastname: He
authors.forename: Tung-Tien
authors.initials: TT
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D018859:Hair Follicle / Q000166:cytology / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics
chemical_list: C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases; D020796:Receptor, Platelet-Derived Growth Factor alpha; D016203:CDC2 Protein Kinase