title: Spelt (Triticum spelta L.) and winter wheat (Triticum aestivum L.) wholemeals have similar sterol profiles, as determined by quantitative liquid chromatography and mass spectrometry analysis.
abstract: From a nutritional point of view, cereal lipids include valuable molecules, such as essential fatty acids, phytosterols, and fat-soluble vitamins. Spelt (Triticum spelta L.) is an alternative hulled bread cereal mostly grown in Belgium, where it is mainly intended for animal feed but should increasingly be used for human consumption. The present research focused on phytosterol quantification by LC/APCI-MS2 in saponified wholemeal extracts of 16 dehulled spelt and 5 winter wheat (Triticum aestivum L.) varieties grown in Belgium during 2001-2002 at the same location. Glycosylated sterols and free and formerly esterified sterols could be determined in saponified extracts. Results show that the mean phytosterol content is comparable in both cereals (whereas other lipids, such as oleic and linoleic acids, are increased in spelt wholemeal): spelt extract has, on average, 527.7 microg of free and esterified sterols g(-1) of wholemeal and 123.8 microg of glycosylated sterols g(-1) of wholemeal versus 528.5 and 112.6 microg x g(-1) in winter wheat (values not corrected for recoveries). This is the first report on the application and validation of an LC/MS2 method for the quantification of phytosterols in spelt and winter wheat.
authors.affiliation: Moulin de Hollange, Rue de Chaumont 5, 6637 Fauvillers, Belgium. ruibal@bnut.ucl.ac.be
authors.forename: Nike L
authors.initials: NL
authors.lastname: Ruibal-Mendieta
authors.forename: Raoul
authors.initials: R
authors.lastname: Rozenberg
authors.forename: Dominique L
authors.initials: DL
authors.lastname: Delacroix
authors.forename: Géraldine
authors.initials: G
authors.lastname: Petitjean
authors.forename: Adrien
authors.initials: A
authors.lastname: Dekeyser
authors.forename: Chiara
authors.initials: C
authors.lastname: Baccelli
authors.forename: Carole
authors.initials: C
authors.lastname: Marques
authors.forename: Nathalie M
authors.initials: NM
authors.lastname: Delzenne
authors.forename: Marc
authors.initials: M
authors.lastname: Meurens
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Habib-Jiwan
authors.forename: Joëlle
authors.initials: J
authors.lastname: Quetin-Leclercq
mesh_terms: D001530:Belgium; D002853:Chromatography, Liquid; D006031:Glycosylation; D013058:Mass Spectrometry; D010840:Phytosterols / Q000032:analysis*; D013045:Species Specificity; D014908:Triticum / Q000737:chemistry*
chemical_list: D010840:Phytosterols