title: The importance of cyclin D1 and Ki67 expression on the biological behavior of pancreatic adenocarcinomas.
abstract: The aim of this study was to evaluate the role of cyclin D1 and Ki67 proteins involved in cell-cycle control as a prognostic factor in pancreatic carcinomas. We examined formalin-fixed, paraffin-embedded material from 59 pancreatic adenocarcinomas, for which appropriate clinical and prognostic data were available. The standard streptavidin biotin immunoperoxidase method was used for immunostaining with cyclin D1 and Ki67. The extent of positive nuclear and cytoplasmic cyclin D1 staining was graded semiquantitatively. Ki67 reactivity was quantified and expressed as the percentage of stained nuclei. Staining with cyclin D1 and Ki67 was compared with histopathological prognostic features, and their relation with survival was also tested statistically. Patients whose tumors were cyclin D1-positive showed perineural invasion significantly more frequently than did patients with cyclin D1-negative tumors at the immunohistochemical level. In addition, tumors with lymphatic vessel invasion and without showed a significant difference in terms of cytoplasmic cyclin D1 staining. Ki67 indices were statistically different in stage groups. There was a significant and direct correlation between Ki67 index and nuclear cyclin D1 staining scores. No relation with survival was found. Our results suggest that cell-cycle proteins do not directly affect the prognosis of patients with pancreatic adenocarcinoma. Conversely, cyclin D1-positive tumors tend to have perineural invasion more frequently. In addition, lymph vessel invasion is another factor related to cyclin D1 reactivity of the cells. Ki67 indices differ statistically in stage groups.
authors.affiliation: Department of Pathology, Medical School, Dokuz Eylül University, Inciralti, Izmir 35340, Turkey. banu.lebe@deu.edu.tr
authors.forename: Banu
authors.initials: B
authors.lastname: Lebe
authors.forename: Ozgül
authors.initials: O
authors.lastname: Sağol
authors.forename: Cağnur
authors.initials: C
authors.lastname: Ulukuş
authors.forename: Ahmet
authors.initials: A
authors.lastname: Coker
authors.forename: Sedat
authors.initials: S
authors.lastname: Karademir
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Astarcioglu
authors.forename: Ali
authors.initials: A
authors.lastname: Küpelioğlu
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Astarcioğlu
authors.forename: Funda
authors.initials: F
authors.lastname: Obuz
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D019938:Cyclin D1 / Q000096:biosynthesis*; D005260:Female; D049669:History, 16th Century; D049670:History, 17th Century; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D019938:Cyclin D1