Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D.
We characterized two novel members of the RAET1/ULBP gene cluster, RAET1E and RAET1G. The encoded proteins were similar to the ULBP in their class I-like alpha1 and alpha2 domains, but differed in that, instead of being GPI-anchored, their sequences were type 1 membrane-spanning molecules. Both proteins were capable of being expressed at the cell surface. Both proteins bound the activating receptor NKG2D, and RAET1G bound the human CMV protein UL16. The expression of diverse NKG2D-binding molecules in different tissues and with different properties is consistent with multiple modes of infection- or stress-induced activation.
Bacon
Louise
L
Department of Pathology, Cambridge Institute for Medical Research, Addenbrookes Hospital, Cambridge, United Kingdom. lb117@cam.ac.uk
Eagle
Robert A
RA
Meyer
Martina
M
Easom
Nicholas
N
Young
Neil T
NT
Trowsdale
John
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D003587:Cytomegalovirus / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D055655:NK Cell Lectin-Like Receptor Subfamily K; D010802:Phylogeny; D020033:Protein Isoforms; D011971:Receptors, Immunologic / Q000378:metabolism*; D055607:Receptors, Natural Killer Cell; D014764:Viral Proteins / Q000378:metabolism
D002352:Carrier Proteins; D015395:Histocompatibility Antigens Class I; C527085:KLRK1 protein, human; D008024:Ligands; D008565:Membrane Proteins; D055655:NK Cell Lectin-Like Receptor Subfamily K; D020033:Protein Isoforms; C488760:RAET1E protein, human; C488761:RAET1G protein, human; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell; D014764:Viral Proteins