Instability of pancuronium in postmortem blood and liver taken after a fatal intramuscular Pavulon injection.
The present study was designed to determine the stability of pancuronium in postmortem blood and liver during storage. Results were obtained using the method by Kerskes et al. [C.H.M. Kerskes, K.J. Lusthof, P.G.M. Zweipfenning, J.P. Franke, The detection and identification of quaternary nitrogen muscle relaxants in biological fluids and tissues by ion-trap LC-ESI-MS, J. Anal. Toxicol. 26 (2002) 29-34.], modified and validated in our laboratory. Target analytes were isolated after enzymatic hydrolysis followed by solid phase extraction (BondElut C18 column). Internal standardisation was carried out using laudanosine and the target ions were monitored by LC-ESI-MS (monitoring ions m/z 358 for IS and 286 for pancuronium). Materials were taken from a 46-year-old woman, who had been found dead. A syringe (2 ml) and an empty ampoule of Pavulon (4 mg/2 mL) were found in her hand. The residual volume of fluid in the syringe was 0.7 ml. An autopsy was performed six days after death. It revealed a needle mark on the left thigh. Postmortem materials (muscle from the injection site, blood and liver) and the syringe with fluid were stored for four months in a freezer at -20 degrees C. The initial pancuronium concentrations were 81 ng/mL in blood and 532 ng/g in liver. The analyte was stable when stored at -20 degrees C in blood even up to seven months. In liver samples its concentrations were variable. Pancuronium in blood stored at 20 degrees C underwent degradation very rapidly. After three months of storage these blood samples had concentrations not greater about 10% of the initial value. The degradation patterns of pancuronium depended on temperature and the biological matrix.
Kała
Maria
M
Institute of Forensic Research, ul. Westerplatte 9, 31-033 Cracow, Poland. mkala@ies.krakow.pl
Lechowicz
Wojciech
W
D004355:Drug Stability; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007273:Injections, Intramuscular; D008099:Liver / Q000737:chemistry*; D008875:Middle Aged; D003473:Neuromuscular Nondepolarizing Agents / Q000032:analysis* / Q000506:poisoning; D010197:Pancuronium / Q000032:analysis* / Q000506:poisoning; D015203:Reproducibility of Results; D012131:Respiratory Insufficiency / Q000139:chemically induced; D013048:Specimen Handling; D013696:Temperature; D013848:Thigh
D003473:Neuromuscular Nondepolarizing Agents; D010197:Pancuronium