title: Cloning of cDNAs encoding multiple subtypes of feline interferon-alpha from the feline epitherial cell line.
abstract: Mammalian interferon (IFN)-alpha consists of a 23-amino acid signal peptide and a 166-amino acid mature protein. Feline (Fe) IFN-alpha has an extra unique molecule consisting of a 171-amino acid mature protein with a 5-amino acid insertion. We cloned eight new subtypes of cDNA encoding FeIFN- alpha from a feline epithelial cell line. Among all the FeIFN-alpha subtypes, including six that have previously been reported, the variations were found to be far less than those of IFN-alphas of other animals.
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Japan.
authors.forename: Ayuko
authors.initials: A
authors.lastname: Nagai
authors.forename: Osamu
authors.initials: O
authors.lastname: Taira
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hiramatsu
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
authors.forename: Setsuo
authors.initials: S
authors.lastname: Arai
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Sato
authors.forename: Katsushige
authors.initials: K
authors.lastname: Nakano
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Maehara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells; D016898:Interferon-alpha / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D016898:Interferon-alpha