Standard operating procedure for immunuslotblot assay for analysis of DNA/sulfur mustard adducts in human blood and skin.
A standard operating procedure has been developed for an immunoslotblot assay of sulfur mustard adducts to DNA in human blood and skin for use in a field laboratory. A minimum detectable level of exposure of human blood in vitro (> or = 50 nM) sulfur mustard is feasible with the assay. In the case of human skin, a 1 s exposure to saturated sulfur mustard vapor (830 mg/m(-3)) could still be detected.
van der Schans
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TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA Rijswijk, The Netherlands.
Mars-Groenendijk
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de Jong
L P A
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Benschop
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Noort
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D
D002619:Chemical Warfare Agents / Q000737:chemistry / Q000506:poisoning*; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting / Q000592:standards*; D007962:Leukocytes / Q000737:chemistry; D008887:Military Medicine / Q000379:methods; D009151:Mustard Gas / Q000737:chemistry / Q000506:poisoning*; D012016:Reference Values; D012867:Skin / Q000737:chemistry / Q000187:drug effects*
D002619:Chemical Warfare Agents; D018736:DNA Adducts; D009151:Mustard Gas