Novel targets of ANG II regulation in mouse heart identified by serial analysis of gene expression.
Although the central role of ANG II in cardiovascular homeostasis is well appreciated, the molecular circuitry of its many actions is not completely understood. With the use of serial analysis of gene expression to assess global transcriptional changes in the heart of mice after continuous 7-day ANG II administration, we identified patterns of gene expression indicative of cardiac remodeling, including coordinate regulation of genes previously described in a context of processes associated with hypertrophy and fibrosis. In addition, we discovered several novel ANG II targets, including characterized genes of known function, recently annotated genes of unknown function, and the putative genes not yet present in current databases. The serial analysis of gene expression approach to assess the role of ANG II presented in this report provides new venues for inquiries into ANG II-mediated cardiac function.
Schwartz
Faina
F
Dept. of Medicine, Genetics Program, Boston Univ. School of Medicine, 715 Albany St., L-320, Boston, MA 02118, USA. fschw@bu.edu
Duka
Arvi
A
Duka
Irena
I
Cui
Jing
J
Gavras
Haralambos
H
D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D000804:Angiotensin II