title: [Thirty base pair (30 bp) deletion in latent membrane protein 1 oncogene in lymphoepithelial carcinoma of salivary glands].
abstract: OBJECTIVE
  To investigate 30 bp deletion of latent membrane protein (LMP)-1 gene in lymphoepithelial carcinoma (LEC) of the salivary glands and to determine the frequency of this deletion.
  
  
  METHODS
  Forty-six cases of salivary gland LEC were investigated by PCR to explore the site specific, 30 bp deletion in the 3' terminal region of LMP-1 gene. To guarantee amplifiable DNA extracted from paraffin-embedded tissue sections, PCR amplification of a house-keeping gene (beta-actin) was performed simultaneously. In addition, DNA sequencing of the PCR product was performed in representative cases.
  
  
  RESULTS
  Although amplifiable DNA was obtained in 42 of the 46 specimens, as indicated by beta-actin gene amplification, successful amplification of LMP-1 gene was achieved in 35/42 (83.3%) cases. Two types of PCR products of LMP-1 gene were observed and confirmed by DNA sequencing. A wild-type PCR product (316 bp) was present in 31 cases (88.6%) and only 4 cases (11.4%) showed an aberrant 286 bp PCR product, corresponding to the 3' terminal 30 bp deletion of the gene.
  
  
  CONCLUSIONS
  Site-specific 30 bp deletion of LMP-1 gene is not a common feature of salivary gland LEC.
authors.affiliation: Department of Pathology, Cancer Hospital, Fudan University, Shanghai 210032, China. tzchen766@yahoo.com.cn
authors.forename: Tong-Zhen
authors.initials: TZ
authors.lastname: Chen
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.forename: Xiong-Zeng
authors.initials: XZ
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics / Q000473:pathology* / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology / Q000821:virology; D017353:Gene Deletion; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009856:Oncogene Proteins, Viral; D014763:Viral Matrix Proteins