Covalent display of oligosaccharide arrays in microtiter plates.
A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 muM).
Bryan
Marian C
MC
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Fazio
Fabio
F
Lee
Hing-Ken
HK
Huang
Cheng-Yuan
CY
Chang
Aileen
A
Best
Michael D
MD
Calarese
Daniel A
DA
Blixt
Ola
O
Paulson
James C
JC
Burton
Dennis
D
Wilson
Ian A
IA
Wong
Chi-Huey
CH
D002240:Carbohydrate Sequence; D020650:Combinatorial Chemistry Techniques; D004229:Dithiothreitol / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000737:chemistry
D009844:Oligosaccharides; D014230:Triazoles; D004229:Dithiothreitol