title: Direct identification of Mycobacterium haemophilum in skin lesions of immunocompromised patients by PCR-restriction endonuclease analysis.
abstract: PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 weeks required for culture isolation and identification by conventional methods.
authors.affiliation: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608. gptwsx@sgh.com.sg
authors.forename: S X
authors.initials: SX
authors.lastname: Wang
authors.forename: L H
authors.initials: LH
authors.lastname: Sng
authors.forename: H N
authors.initials: HN
authors.lastname: Leong
authors.forename: B H
authors.initials: BH
authors.lastname: Tan
mesh_terms: D004262:DNA Restriction Enzymes / Q000494:pharmacology; D006801:Humans; D016867:Immunocompromised Host; D018393:Mycobacterium haemophilum / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000293:injuries / Q000382:microbiology*
chemical_list: D004262:DNA Restriction Enzymes