Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein.
OBJECTIVE
To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and proliferation activity effects induced these cells by Amyloid beta-Protein (Abeta-43).


METHODS
1) PC12 cells in logarithmic growth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-beta-NGF and cultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups: control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Abeta in the four groups were 0 micromol/L, 1.25 micromol/L, 2.5 micromol/L and 5 micromol/L, respectively. The cells were harvested at 24, 48 and 72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted to examine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Abeta with different concentrations was added. The final concentrations of Abeta were 0 micromol/L, 1.25 micromol/L, 2.5 micromol/L and 5 micromol/L, respectively. After the cells were incubated in an atmosphere of 5% CO2 at 37 degrees C in an incubator for 72 h, the OD values were examined.


RESULTS
1) Neuronal differentiated PC12 cell lines were successfully established. 2) Flow cytometric examination indicated that Abeta (1.25, 2.5, and 5.0 micromol/L) could effectively induce apoptosis of neuronal-differentiated cells at the 24 h, 48 h and 72 h time points. 3) Abeta (0-5.00 micromol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of the PC12 cells after a 72 h interacting process.


CONCLUSIONS
This investigation revealed successful neuronal differentiation of the PC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Abeta was observed and the influence of Abeta on induced proliferation of PC12 cells by Rat-beta-NGF was revealed. This study may provide basis for future research on the molecular cure of AD and interdiction of AD evolution.
Ruo
Ben-Yan
BY
Department of Neurology, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China. lobenyan@263.net
Xu
Zeng-Bin
ZB
Chen
Zhi
Z
Chen
Feng
F
Tang
Min
M
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats
D016229:Amyloid beta-Peptides; D020932:Nerve Growth Factor