[C-reactive protein inhibits the expression of hypoxia-inducible factor-1alpha in human umbilical vein endothelial cells in hypoxic condition].
OBJECTIVE
To investigate the effect of C-reactive protein (CRP) on expression of hypoxia-inducible factor-1alpha (HIF-1alpha).


METHODS
Using CoCl(2) (100 micromol/L) to simulate hypoxic condition for culturing human umbilical vein endothelial cells (HUVECs), we examined the effects of CRP on expression of HIF-1alpha. The proteins were extracted from the cells after a 24-hour exposure of the cells to CRP of varied concentrations in the presence of CoCl(2), and Western blotting was performed for quantification of HIF-1alpha expression, the results were analyzed statistically with SPSS software.


RESULTS
CRP at the concentration of 5 microg/ml decreased the expression of HIF-1alpha (P<0.001), producing the maximum inhibitory effect at the concentration of 100 microg/ml, an effect exhibiting dose-dependence.


CONCLUSIONS
CRP inhibits the expression of HIF-1alpha in HUVECs subjected to hypoxic condition, which serves as an important evidence for the inhibitory effect of CRP on angiogenesis in hypoxic condition.
Song
Yun-feng
YF
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yfsong789@yahoo.com.cn
Wu
Ping-sheng
PS
Lai
Wen-yan
WY
D015153:Blotting, Western; D002097:C-Reactive Protein / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000032:analysis*; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*
D004268:DNA-Binding Proteins; C497442:HIF1A protein, human; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors; D003035:Cobalt; D002097:C-Reactive Protein; C018021:cobaltous chloride