title: Neutrophil CD177 (NB1 gp, HNA-2a) expression is increased in severe bacterial infections and polycythaemia vera.
abstract: The NB1 glycoprotein (CD177, HNA-2a antigen) is exclusively expressed on human neutrophils. As the clinical significance of CD177 expression is unknown, we investigated its expression in healthy individuals before and after stimulation with granulocyte colony-stimulating factor (G-CSF), in patients with rheumatoid arthritis, viral hepatitis, severe bacterial infections and polycythaemia vera. Expression was quantitatively determined by flow cytometry and by real time polymerase chain reaction. Only G-CSF-stimulated individuals and patients with severe bacterial infections and polycythaemia showed a significantly (P < 0.001) increased CD177 expression compared with healthy individuals, indicating that neutrophil CD177 expression can increase significantly in certain clinical conditions.
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine & Department of Internal Medicine, Justus-Liebig University, Giessen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Göhring
authors.forename: J
authors.initials: J
authors.lastname: Wolff
authors.forename: W
authors.initials: W
authors.lastname: Doppl
authors.forename: K L
authors.initials: KL
authors.lastname: Schmidt
authors.forename: K
authors.initials: K
authors.lastname: Fenchel
authors.forename: H
authors.initials: H
authors.lastname: Pralle
authors.forename: U
authors.initials: U
authors.lastname: Sibelius
authors.forename: J
authors.initials: J
authors.lastname: Bux
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006525:Hepatitis, Viral, Human / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D011087:Polycythemia Vera / Q000276:immunology*; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D016179:Granulocyte Colony-Stimulating Factor