[Human gastric tissues coexpress two different splicing cholecystokinin-B/gastrin receptors].
This study was conducted to explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant (CCKBRi4sv) are expressed in human gastric carcinomas cell line and tissues, and to find out their relationship with the development and progression of human gastric carcinoma. The mRNA expression levels of CCKBRwt and CCKBRi4sv were detected in 30 human gastric carcinomas and normal tissues adjacent to cancer, 10 gastritis specimens, 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells. The results revealed that the transcripts of CCKBRwt and CCKBRi4sv were observed in all of the human gastric specimens tested, but only CCKBRwt was expressed in gastric cancer cell line SGC-7901 cells. The expression levels of the two receptors were not correlated with the differentiation and metastases of gastric cancers. From the results, we infer that human gastric tissues simultaneously express CCKBRwt and CCKBRi4sv, and CCKBRi4sv may have unknown physiological functions in gastric epithelial cells.
Zhou
Jianjiang
J
Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Sichuan University, Chengdu 610041, China.
Hu
Jiankun
J
Chen
Zhixin
Z
Wang
Wenling
W
Zhang
Qunzhou
Q
Chen
Manling
M
D001483:Base Sequence; D004847:Epithelial Cells / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000096:biosynthesis* / Q000235:genetics; D005756:Gastritis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005755:Gastrins; D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B