title: Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA.
abstract: We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.
authors.affiliation: Department of Pediatrics, Box 8116, Washington University School of Medicine at St. Louis Children's Hospital, One Children's Place, St. Louis, MO 63110, USA. buller@kids.wustl.edu
authors.forename: Richard S
authors.initials: RS
authors.lastname: Buller
authors.forename: Gregory
authors.initials: G
authors.lastname: Storch
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral