title: In vitro effect of a buffered chelating agent and neomycin or oxytetracycline on bacteria associated with diseases of fish.
abstract: The antimicrobial agents used to treat bacterial fish diseases are archaic, and their uses may result in the emergence of drug-resistant bacterial strains. This study evaluated the in vitro antimicrobial activity of combinations of Tricide and neomycin or oxytetracycline on common disease-causing bacteria of fish and its possible use as an alternative treatment of these diseases. Tricide solutions containing of 8 mM United States Pharmacopeia (USP) disodium ethylenediaminetetraacetate dehydrate (chelator) and 20 mM USP 2-amino-2-hydroxymethyl-1,3-propanediol (buffer) potentate the antimicrobial action of neomycin and oxytetracycline when reacted in vitro with Aeromonas hydrophila, Streptococcus iniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Serial passage of the test organisms in Tricide or Tricide and neomycin or oxytetracycline did not result in the development of resistant forms. Combinations of Tricide and neomycin or oxytetracycline reduced the amount of antibiotics necessary for fish therapy, render drug-resistant bacteria sensitive to antimicrobial therapy, may be used to decontaminate recently shipped fish, and should reduce the formation of antibiotic-resistant forms.
authors.affiliation: Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602-7386, USA. wooleyr@vet.uga.edu
authors.forename: Richard E
authors.initials: RE
authors.lastname: Wooley
authors.forename: Branson W
authors.initials: BW
authors.lastname: Ritchie
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Burnley
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000662:veterinary*; D002021:Buffers; D002614:Chelating Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009355:Neomycin / Q000494:pharmacology; D010118:Oxytetracycline / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D002614:Chelating Agents; D011409:Propylene Glycols; D004492:Edetic Acid; C019250:2-amino-2-methyl-1,3-propandiol; D009355:Neomycin; D010118:Oxytetracycline