Neurotrophin-3 and TrkC are expressed in the outflow tract of the developing chicken heart.
Transcripts encoding trkC and full-length (catalytic) TrkC receptors were detected in the outflow tract of the chicken heart during early development (stage 17; embryonic day [E] 2.5) before the start of septation. Expression of trkC mRNA persisted through early septation (stage 25, E4.5-E5) but was no longer evident by the end of septation (stage 34, E8). Neurotrophin-3 (NT-3) mRNA was also shown to be present in the outflow tract throughout cardiac development. Quail-chick chimeras were used to confirm that cardiac neural crest cells were not present in the outflow tract at stage 17 (E2.5). Our results show that NT-3 interacts with cells in the outflow tract that are not of neural crest origin. This finding indicates that, in addition to effects on neural crest cells, NT-3 may be important for cardiac development due to its interaction with cells in the outflow tract such as those arising from the secondary heart field.
Bernd
Paulette
P
Department of Anatomy, State University of New York, Downstate Medical Center, Brooklyn, 11203, USA. paulette.bernd@downstate.edu
Miles
Kathryn
K
Rozenberg
Inna
I
Borghjid
Saihan
S
Kirby
Margaret L
ML
D000818:Animals; D002384:Catalysis; D002645:Chickens; D002678:Chimera; D006321:Heart / Q000196:embryology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009206:Myocardium / Q000378:metabolism*; D009432:Neural Crest / Q000196:embryology; D020933:Neurotrophin 3 / Q000096:biosynthesis* / Q000502:physiology*; D011784:Quail; D012333:RNA, Messenger / Q000378:metabolism; D020812:Receptor, trkC / Q000096:biosynthesis* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D020933:Neurotrophin 3; D012333:RNA, Messenger; D020812:Receptor, trkC