Effects of androgen manipulation on alpha1-adrenoceptor subtypes in the rat seminal vesicle.
This study analyses possible changes during surgical and chemical castration in the expression and pharmacological characteristics of alpha1-adrenoceptor in adult rat seminal vesicle. Ribonuclease protection assays indicated that alpha1a--was the predominant mRNA, while alpha1b-and alpha1d-adrenoceptor transcripts were detected in lower abundance in this tissue. alpha1a-adrenoceptor mRNA expression presented a complex dependency on androgens, while alpha1b- and alpha1d-adrenoceptor transcripts were both upregulated with surgical and chemical castration, suggesting a negative modulation by androgens. Testosterone treatment reversed the effects caused by surgical castration. Functional studies confirmed the involvement of alpha1A- and alpha1B-adrenoceptor in the seminal vesicle contractile responses, and suggested that alpha1B-induced contractile response was upregulated after castration. Taken together, the results suggest that alpha1-adrenoceptor expression in seminal vesicle is differentially regulated by the androgen status of the rat.
Mendes
Fúlvio R
FR
Department of Pharmacology, Section of Experimental Endocrinology, UNIFESP-Escola Paulista de Medicina, Rua 03 de maio 100, INFAR, Vila Clementino, 04044-020, São Paulo, Brazil.
Hamamura
Margarete
M
Queiróz
Daniel B C
DB
Porto
Catarina S
CS
Avellar
Maria Christina W
MC
D058668:Adrenergic alpha-1 Receptor Antagonists; D000704:Analysis of Variance; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D017609:Barium Compounds; D002712:Chlorides / Q000502:physiology; D003000:Clonidine / Q000031:analogs & derivatives*; D017373:Cyproterone Acetate / Q000037:antagonists & inhibitors; D004146:Dioxanes; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D009919:Orchiectomy; D009929:Organ Size; D010656:Phenylephrine; D010879:Piperazines; D011799:Quinazolines; D011810:Quinoxalines; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D012669:Seminal Vesicles / Q000378:metabolism* / Q000502:physiology; D013739:Testosterone / Q000097:blood / Q000494:pharmacology
D058668:Adrenergic alpha-1 Receptor Antagonists; D000728:Androgens; D017609:Barium Compounds; D002712:Chlorides; D004146:Dioxanes; D010879:Piperazines; D011799:Quinazolines; D011810:Quinoxalines; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; C024986:barium chloride; C080462:cyclazosin; D010656:Phenylephrine; C051731:chlorethylclonidine; D013739:Testosterone; D017373:Cyproterone Acetate; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; C053428:BMY 7378; D003000:Clonidine