title: Differential pressure resistance in the activity of RNA polymerase isolated from Shewanella violacea and Escherichia coli.
abstract: RNA polymerase was purified from the piezophile Shewanella violacea DSS12, and the transcriptional activity after pressure treatment was compared with that of the mesophile Escherichia coli. Application of pressure at 100 MPa for 30 min reduced the E. coli RNA polymerase activity to 60% of the activity at atmospheric pressure, whereas the S. violacea RNA polymerase maintained full activity, indicating that the S. violacea RNA polymerase is more stable than its E. coli counterpart. This result was supported by the analysis of the strength of subunit interactions of the enzyme from both species, using a high-pressure electrophoresis apparatus, which showed that a pressure of 140 MPa caused dissociation of E. coli RNA polymerase but not that of S. violacea RNA polymerase. On the other hand, the core enzyme of S. violacea RNA polymerase, which lacked the sigma70 factor, was dissociated at 140 MPa. These results suggest that the sigma70 factor is required for stabilization of S. violacea RNA polymerase under high-pressure conditions. In this paper, we provide in vitro evidence for piezoadaptation at the transcriptional level, using purified RNA polymerase from cells of S. violacea and E. coli.
authors.affiliation: The DEEPSTAR Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawano
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakasone
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods; D018360:Crystallography, X-Ray; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008957:Models, Genetic; D008958:Models, Molecular; D011312:Pressure; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012329:RNA, Bacterial / Q000378:metabolism; D020592:Shewanella / Q000201:enzymology*; D012808:Sigma Factor / Q000737:chemistry; D013045:Species Specificity; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases