title: Fabrication of alternating polycation and albumin multilayer coating onto stainless steel by electrostatic layer-by-layer adsorption.
abstract: Multilayer films consisting of polyethylenimine (PEI) and albumin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly of the PEI and albumin. The process of electrostatic self-assembly of PEI/albumin was monitored by125I radiolabeling, electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM). The EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. 125I radiolabeling experiments indicated that less than 10% albumin was eluted by PBS in 45 days. Static platelet adhesion experiments indicated that the PEI/albumin deposited on stainless steel could resist platelet adhesion effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices.
authors.affiliation: Department of Polymer Science, Zhejiang University, Hangzhou 310027, China. jianji@mail.hz.zj.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Tan
authors.forename: De-Zeng
authors.initials: DZ
authors.lastname: Fan
authors.forename: Fu-Yu
authors.initials: FY
authors.lastname: Sun
authors.forename: M A
authors.initials: MA
authors.lastname: Barbosa
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000737:chemistry* / Q000378:metabolism; D017097:Electric Impedance; D004583:Electrons; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D010973:Platelet Adhesiveness; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry; D013057:Spectrum Analysis; D013193:Stainless Steel / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D000418:Albumins; D008051:Lipid Bilayers; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D013193:Stainless Steel