title: Dynamics of CaCdc10, a septin of Candida albicans, in living cells and during infection.
abstract: The morphogenetic program in the pathogenic fungus Candida albicans, including the dimorphic transition, is an interesting field of study, not only because it is absent in the commonly used model yeast Saccharomyces cerevisiae, but because of the close relationship between hyphal development and virulence of C. albicans. We studied one of the most important aspects of fungal morphogenesis--the septin ring--in C. albicans. By using a fusion construct to green fluorescent protein (GFP), the subcellular localization and dynamics of C. albicans Cdc10 in the different morphologies that this fungus is able to adopt was identified. The localization features reached were contrasted and compared with the results obtained from Candida cells directly extracted from an animal infection model under environmental conditions as similar as possible to the physiological conditions encountered by C. albicans during host infection.
authors.affiliation: Institute for Biochemical Microbiology, University of Salamanca-CSIC, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: González-Novo
authors.forename: Javier
authors.initials: J
authors.lastname: Jiménez
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: García
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ríos-Serrano
authors.forename: Jesús
authors.initials: J
authors.lastname: Pla
authors.forename: Antonio
authors.initials: A
authors.lastname: Jiménez
authors.forename: Miguel
authors.initials: M
authors.lastname: Sánchez-Pérez
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000235:genetics; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics; D020558:GTP Phosphohydrolases; D049452:Green Fluorescent Proteins; D025301:Hyphae / Q000254:growth & development; D008164:Luminescent Proteins / Q000235:genetics; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D058112:Septins; D014157:Transcription Factors
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; D049452:Green Fluorescent Proteins; C512892:CDC10 protein, S cerevisiae; D020558:GTP Phosphohydrolases; C512895:Sept7 protein, mouse; D058112:Septins