Percutaneous absorption of 5 glycol ethers through human skin in vitro.
Absorption across full thickness human skin was evaluated in vitro for five selected glycol ethers. Skin membranes were settled on static diffusion cells and both neat and 50% water diluted glycol ethers were applied on the donor chamber for 8 h. The amount of glycol ethers permeated into the receptor fluid was measured by gas chromatograph equipped with flame ionization detector (GC-FID). For neat solvents, permeation coefficient Kp ranged from 0.06 to 0.83 cm h(-1) 10(-3) respectively for DEGBEA and EGMEA while for 50% v/v diluted glycol ethers it varied from 0.08 to 1.81 cm h(-1) 10(-3) respectively for DPGME and EGMEA. These experiments show a statistically significant (Student's t-test, P <0.05) increase in permeation coefficients from neat to 50% water diluted glycol ethers and the same trend can be observed in fluxes and lag times. Only DPGME show an opposite behaviour. These results confirm the good ability of these solvents of permeating the skin and show that they could represent a risk for their potential dermal absorption both for workers and for occasional exposures, since the average lag time is 1.57 h.
Venier
M
M
Department of Chemical Sciences, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy. venier@dsch.univ.trieste.it
Adami
G
G
Larese
F
F
Maina
G
G
Renzi
N
N
D002849:Chromatography, Gas; D004987:Ethers / Q000032:analysis / Q000493:pharmacokinetics*; D005026:Ethylene Glycols / Q000032:analysis / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D016273:Occupational Exposure; D010539:Permeability; D011409:Propylene Glycols / Q000032:analysis / Q000493:pharmacokinetics*; D012869:Skin Absorption; D012997:Solvents / Q000493:pharmacokinetics*
D004987:Ethers; D005026:Ethylene Glycols; D011409:Propylene Glycols; D012997:Solvents