The C-type lectin homologue gene (EP153R) of African swine fever virus inhibits apoptosis both in virus infection and in heterologous expression.
The open reading frame EP153R of African swine fever virus (ASFV) encodes a nonessential protein that has been involved in the hemadsorption process induced in virus-infected cells. By the use of a virus deletion mutant lacking the EP153R gene, we have detected, in several virus-sensitive cells, increased levels of caspase-3 and cell death as compared with those obtained after infection with the parental BA71V strain. Both transient and stable expression of the EP153R gene in Vero or COS cells resulted in a partial protection of the transfected lines from the apoptosis induced in response to virus infection or external stimuli. The presence of gene EP153R resulted in a reduction of the transactivating activity of the cellular protein p53 in Vero cell cultures in which apoptosis was induced by virus infection or staurosporine treatment. This is to our knowledge the first description of a viral C-type lectin with anti-apoptotic properties.
Hurtado
Carolina
C
Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Granja
Aitor G
AG
Bustos
María J
MJ
Nogal
María L
ML
González de Buitrago
Gonzalo
G
de Yébenes
Virginia G
VG
Salas
María L
ML
Revilla
Yolanda
Y
Carrascosa
Angel L
AL
D000357:African Swine Fever / Q000378:metabolism / Q000821:virology; D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral / Q000502:physiology*; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000821:virology; D016366:Open Reading Frames; D013552:Swine; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000378:metabolism
D061945:Caspase Inhibitors; D004792:Enzyme Precursors; D037181:Lectins, C-Type; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; D020169:Caspases