title: Validation of a PCR-based method for detection of food-borne thermotolerant campylobacters in a multicenter collaborative trial.
abstract: A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.
authors.affiliation: Danish Institute for Food and Veterinary Research (DFVF), 27 Bülowsvej, DK-1790 Copenhagen V, Denmark.
authors.forename: M H
authors.initials: MH
authors.lastname: Josefsen
authors.forename: N
authors.initials: N
authors.lastname: Cook
authors.forename: M
authors.initials: M
authors.lastname: D'Agostino
authors.forename: F
authors.initials: F
authors.lastname: Hansen
authors.forename: M
authors.initials: M
authors.lastname: Wagner
authors.forename: K
authors.initials: K
authors.lastname: Demnerova
authors.forename: A E
authors.initials: AE
authors.lastname: Heuvelink
authors.forename: P T
authors.initials: PT
authors.lastname: Tassios
authors.forename: H
authors.initials: H
authors.lastname: Lindmark
authors.forename: V
authors.initials: V
authors.lastname: Kmet
authors.forename: M
authors.initials: M
authors.lastname: Barbanera
authors.forename: P
authors.initials: P
authors.lastname: Fach
authors.forename: S
authors.initials: S
authors.lastname: Loncarevic
authors.forename: J
authors.initials: J
authors.lastname: Hoorfar
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002645:Chickens; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine