Algae extract protection effect on oxidized protein level in human stratum corneum.
Modification of proteins by reactive oxygen species is implicated in different disorders. The proteasome is a multicatalytic proteinase in charge of intracellular protein turnover and of oxidized proteins degradation. Consequently, proteasome function is very important in controlling the level of altered proteins in eukaryotic cells. Evidence for a decline in proteasome activity during skin photo-aging has been provided in Bulteau et al. in 2002. The ability of a lipid algae extract (Phaeodactylum tricornutum) to stimulate 20S proteasome peptidase activities was described by Nizard et al. in 2001. Furthermore, keratinocytes treated with Phaeodactylum tricornutum extract and then UVA and UVB irradiated, exhibited a sustained level of proteasome activity comparable to the one of nonirradiated cells. The level of modified proteins can be quantified by measurement of protein carbonyl content (Oxyblot technique), which has been shown to increase with aging and other disorders. In this paper, it is described that, in the presence of this lipid algae extract, the level of oxidized proteins is reduced, as assessed by the Oxyblot technique. These results are obtained both with culture of human keratinocytes and stratum corneum skin cells (obtained by stripping) from human volunteers. Altogether, these results argue for the presence of compounds in this algae extract that have a stimulating and/or protective effect on proteasome activity, resulting in a decreased level of protein oxidation.
Nizard
Carine
C
LVMH Branche Parfums et Cosmetiques, 45804, Saint Jean de Braye, Cedex, France. carine.nizard-u505@bhdc.jussieu.fr
Poggioli
Sylvie
S
Heusèle
Catherine
C
Bulteau
Anne-Laure
AL
Moreau
Marielle
M
Saunois
Alex
A
Schnebert
Sylvianne
S
Mahé
Christian
C
Friguet
Bertrand
B
D000328:Adult; D000368:Aged; D000375:Aging; D000975:Antioxidants / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D056890:Eukaryota / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000378:metabolism*; D050356:Lipid Metabolism; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D017382:Reactive Oxygen Species; D012867:Skin / Q000378:metabolism*; D014466:Ultraviolet Rays
D000975:Antioxidants; D009097:Multienzyme Complexes; D017382:Reactive Oxygen Species; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D010100:Oxygen