NuMA in rat testis--evidence for roles in proliferative activity and meiotic cell division.
NuMA is a well-characterized organizer of the mitotic spindle, which is believed to play a structural role in interphase nucleus. We studied the expression of NuMA in rat seminiferous epithelium in detail. Different stages of the cycle of the seminiferous epithelium were identified using transillumination. Corresponding areas were microdissected and analysed using immunofluorescence, immunohistochemistry, or immunoblotting. NuMA was expressed in Sertoli cells, proliferating type A and B spermatogonia, and early spermatids but it was absent in late spermatids and mature spermatozoa. Interestingly, NuMA-positive primary spermatocytes lost their nuclear NuMA at the beginning of long-lasting prophase of the first meiotic division. A strong expression was again observed at the end of the prophase and finally, a redistribution of NuMA into pole regions of the meiotic spindle was observed in first and second meiotic divisions. In immunoblotting, a single 250-kDa protein present in all stages of the rat seminiferous epithelial cycle was detected. Our results show that NuMA is not essential for the organization of nuclear structure in all cell types and suggest that its presence is more likely connected to the proliferation phase of the cells. They also suggest that NuMA may play an important role in meiotic cell division.
Taimen
Pekka
P
Department of Anatomy, University of Turku, FIN-20520 Turku, Finland. pekka.taimen@utu.fi
Parvinen
Martti
M
Osborn
Mary
M
Kallajoki
Markku
M
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008540:Meiosis / Q000502:physiology*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011418:Prophase / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012670:Seminiferous Epithelium / Q000166:cytology / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism; D008941:Spindle Apparatus / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000378:metabolism*
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C489081:Numa1 protein, rat