The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin.
To understand the function of the suppressor of cytokine signaling (SOCS)-7, we have looked for proteins interacting with SOCS-7 in a stringent yeast two-hybrid screen of a human leukocyte cDNA-library. We identified the cytoskeletal molecule vinexin as a partner interacting with SOCS-7. Tests with deletion mutants of SOCS-7 demonstrated that a central region of the molecule containing several proline-rich regions, N-terminal to the SH2 domain, was responsible for the binding to vinexin. It is thus likely that one of the SH3 domains of vinexin interacts with a poly-proline region of SOCS-7. The interaction with vinexin was confirmed biochemically as vinexin-alpha was co-precipitated with SOCS-7. Confocal laser-scanning microscopy in HEK293T, MCF-7, and 3T3-L1 cells showed that part of the transfected SOCS-7-green fluorescent protein (GFP) molecules merged with vinexin and with actin. Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton.
Martens
Nele
N
Neuroendocrine Immunology Group, Pharmacology Department, Medical School, Free University of Brussels (VUB), 1090, Belgium. nmartens@farc.vub.ac.be
Wery
Maxime
M
Wang
Ping
P
Braet
Filip
F
Gertler
Arieh
A
Hooghe
Robert
R
Vandenhaute
Jean
J
Hooghe-Peters
Elisabeth L
EL
D041721:3T3-L1 Cells; D000199:Actins / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000502:physiology; D050826:Suppressor of Cytokine Signaling Proteins; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains / Q000502:physiology
D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D009124:Muscle Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C108775:SOCS7 protein, human; C486523:SORBS3 protein, human; C117452:Sorbs3 protein, mouse; D050826:Suppressor of Cytokine Signaling Proteins