title: Herd-level seroprevalence of fasciolosis in cattle in north central Portugal.
abstract: An epidemiological study of Fasciola hepatica in cattle was implemented in the north central region of Portugal. Both an enzyme-linked immunosorbent assay and an egg shedding quantification technique were used in the follow-up of seven herds. Two of these herds were negative and the other five were positive for F. hepatica. A herd cut-off of value of 0.425 optical density was calculated and herd sensitivity (HSe) and herd specificity (HSp) were defined. Three seroprevalence studies were also implemented in the region with stratification by county sub-regions for a period of 18 months. Overall mean herd prevalence in Vagos of 11, 23 and 48% was progressively found for the three studies, respectively.
authors.affiliation: Escola Superior Agrária de Coimbra, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal. toinha@mail.esac.pt
authors.forename: M A P
authors.initials: MA
authors.lastname: Conceição
authors.forename: R M B
authors.initials: RM
authors.lastname: Durão
authors.forename: I M H
authors.initials: IM
authors.lastname: Costa
authors.forename: A
authors.initials: A
authors.lastname: Castro
authors.forename: A C
authors.initials: AC
authors.lastname: Louzã
authors.forename: J C
authors.initials: JC
authors.lastname: Costa
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology*; D003430:Cross-Sectional Studies; D004013:Dicrocoelium / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005211:Fascioliasis / Q000453:epidemiology* / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D005500:Follow-Up Studies; D010270:Parasite Egg Count / Q000662:veterinary; D011174:Portugal / Q000453:epidemiology; D016036:Seroepidemiologic Studies
chemical_list: D000909:Antibodies, Helminth