Brain tumor inhibition in experimental model by restorative immunotherapy with a corpuscular antigen.
In view of the advances in our understanding of anti-tumor immune response, it is now tempting to contemplate the development of immunotherapies for malignant brain tumors, for which no effective treatment exists. Immunotherapy, with agents known as biological response modifiers (BRMs) are thus gaining increasing interest as the fourth modality of treatment. A non-specific BRM, sheep erythrocytes (SRBC) when administered (ip, 7% PCV/V, 0.5 ml) in a group of animals at the end of seventh month of ethylnitrosourea administration, resulted in significant increase in the mean survival time (> 350 days). Studies conducted for growth kinetics pattern with proliferation index and fluorochrome (HO-33342) uptake techniques at the tissue culture level exhibited a regulatory inhibition of the cells isolated from tissue excised from the tumor susceptible area of brain of SRBC treated animals. Moreover, histological examination of brain from animals showed immunomodulatory role of SRBC in experimentally induced brain tumor. Further probe into the mechanisms involving immunological investigations at the cellular level in these animals indicated an augmented and potentiated cell mediated immune response (CMI) as evidenced by enhanced spontaneous rosette forming capacity and cytotoxic activity of lymphocytes and neutrophil (PMN) mediated phagocytosis respectively. The observations suggest that SRBC down regulate malignant growth pattern of experimental brain tumors either by an immunologically enhanced killing of tumor cells and/or by directly inhibiting the tumor growth possibly via a stimulated cytokine network. Thus, a corpuscular antigen, can potentiate CMI response in experimentally induced brain tumor animal model, in which response induced in the periphery are able to mediate anti-tumor effects in the brain.
Gangopaddhyay
Soven Kumar
SK
Immunology Section, Department of Pathology, University College of Medicine, 244B, A.J.C. Bose Road, Kolkata 700020, India.
Sarkar
Susobhan
S
Begum
Zarina
Z
Chaudhuri
Swapna
S
Chaudhuri
Samaresh
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D002455:Cell Division / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005038:Ethylnitrosourea; D005260:Female; D016219:Immunotherapy, Adoptive; D008297:Male; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000941:Antigens; D005038:Ethylnitrosourea