Mass spectrometric identification of RNA binding proteins from dried EMSA gels.
Electrophoretic mobility shift assays (EMSA) are commonly employed for the analysis of nucleic acid/ protein interactions with a native gel system. Here, we report a method to identify RNA binding proteins from a dried EMSA gel by mass spectrometry following autoradiography. Compared to wet gel exposure, our approach resulted in an improved protein identification sensitivity and RNA/protein complex isolation accuracy. The method described here is useful for the large scale characterization of RNA- or DNA-protein complexes.
Stenger
Dominique
D
Institute of Plant Science, Swiss Federal Institute of Technology, Zürich, Switzerland.
Gruissem
Wilhelm
W
Baginsky
Sacha
S
D001345:Autoradiography; D024202:Electrophoretic Mobility Shift Assay; D005782:Gels / Q000737:chemistry; D013058:Mass Spectrometry; D016601:RNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*
D005782:Gels; D016601:RNA-Binding Proteins