Simultaneous determination of four bufadienolides in human liver by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the simultaneous quantitation of four bufadienolides-cinobufotalin, bufalin, cinobufagin and resibufogenin-in human liver was investigated. The procedure involved solid phase extraction of human liver with an Oasis HLB cartridge coupled with reversed-phase HPLC and photodiode array detection. Recoveries obtained from spiked liver for the bufadienolides were better than 70%. The linearity was studied up to 1.2 mg/kg and the detection limits of the method were 0.4 ng for cinobufotalin and bufalin and 0.5 ng for cinobufagin and resibufogenin. The developed method was successfully applied to a lethal poisoning case.
Wang
Zhaohong
Z
School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
Wen
Jiao
J
Zhang
Jizong
J
Ye
Min
M
Guo
Dean
D
D000328:Adult; D002018:Bufanolides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008099:Liver / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002018:Bufanolides