Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices.
The aim of the present study was to investigate the effect of cyclic uniaxial mechanical strain on a human osteoblastic precursor cell line (hFOB 1.19) in three-dimensional type I collagen matrices. Cell seeded collagen constructs were mechanically stretched by a daily application of cyclic uniaxial strain using a special motor-driven apparatus and compared to unstretched controls. Expression of genes involved in cell proliferation and osteoblastic differentiation as well as matrix production were investigated by analyzing the mRNA of histone H4, core binding factor 1, alkaline phosphatase, osteopontin, osteocalcin, and collagen type I (Col I) up to a cultivation period of 3 weeks using real-time PCR. Cyclic stretching of cell seeded Col I matrices at a magnitude occurring in healing bone increased cell proliferation and slightly elevated the expression of nearly all investigated genes over unstrained controls at various time points. It was concluded that mechanical load promotes the proliferation and differentiation of osteoblastic precursor cells in a Col I matrix and that the application of mechanical stimuli may have a beneficial effect on in vitro tissue formation.
Ignatius
A
A
Institute of Orthopaedic Research and Biomechanics, University of Ulm, Helmholtzstrasse 14, Ulm 89081, Germany. anita.ignatius@medizin.uni-ulm.de
Blessing
H
H
Liedert
A
A
Schmidt
C
C
Neidlinger-Wilke
C
C
Kaspar
D
D
Friemert
B
B
Claes
L
L
D001672:Biocompatible Materials / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D019149:Bioreactors; D018786:Bone Substitutes / Q000737:chemistry*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D024042:Collagen Type I / Q000737:chemistry*; D004548:Elasticity; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D024042:Collagen Type I