Liquid chromatographic and mass spectrometric analysis of human serum acid alpha-1-glycoprotein.
Human serum acid alpha-1-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls.
Kremmer
T
T
National Institute of Oncology, Department of Biochemistry, PO Box 21, H 1122 Budapest, Hungary. kremmer@oncol.hu
Szöllösi
E
E
Boldizsár
M
M
Vincze
B
B
Ludányi
K
K
Imre
T
T
Schlosser
G
G
Vékey
K
K
D001769:Blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009961:Orosomucoid / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D009961:Orosomucoid