Liquid chromatographic method for determination of four active saponins from Panax notoginseng in rat urine using solid-phase extraction.
Four major active saponins (ginsenosides Rg1, Rb1, Rd and notoginsenoside R1) in Panax notoginseng were determined in rat urine after oral and intravenous administration of total saponins of P. notoginseng (PNS), and the urine samples were treated with solid-phase extraction (SPE) prior to liquid chromatography. A reversed-phase liquid chromatography system with ultraviolet detection and a Zorbax SB-C18 column was used. The within-day and between-day assay coefficients of variation for the four saponins in urine were less than 7% and the recovery of this method was higher than 85%. Using this method, the excretion profile of the drug in rat urine after administration of PNS was revealed for the first time.
Li
Lie
L
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, PR China.
Zhang
Jin-Lan
JL
Sheng
Yu-Xin
YX
Ye
Guan
G
Guo
Hong-Zhu
HZ
Guo
De-An
DA
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D012503:Saponins / Q000493:pharmacokinetics / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet
D012503:Saponins