title: Coffee-mediated protective effects against directly acting genotoxins and gamma-radiation in mouse lymphoma cells.
abstract: The cytokinesis-block micronucleus test was performed using L5178Y mouse lymphoma cells to ascertain whether or not standard (caffeinated) instant coffee, the commonly consumed polyphenolic beverage with antioxidant activity can protect against chromosomal damage induced by the directly acting agents N-methyl-N-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), methyl methanesulfonate (MMS) and gamma radiation. Our results demonstrated significant reductions in the in vitro genotoxic effects of MNNG, MMC, and MMS following co-treatment of mouse lymphoma cells with standard instant coffee. Subsequently, the comet assay was carried out to assess the effect of coffee co-treatment on the level of DNA damage induced by MMS in mouse lymphoma cells. The results demonstrated a significant reduction in MMS-induced DNA damage following co-treatment with standard instant coffee. Protective effects were observed in mouse lymphoma cells which were treated with coffee immediately after exposure to gamma radiation (1 and 2 Gy). Another experiment showed protection when the mammalian cells were irradiated (0.5 and 1 Gy) midway (at 2 h) during a 4 h coffee treatment. However, the protective effect against the lower dose (0.5 Gy) was not significant. In addition we assessed the modulatory effect of coffee on MNNG-induced apoptotic frequency by flow cytometry. The results revealed only a minor influence of coffee on the frequency of apoptotic cells induced by the test compounds, rendering an increase in sensitivity for apoptosis as a reason for the reduced genomic damage an unlikely or at least incomplete explanation.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Abraham
authors.forename: V
authors.initials: V
authors.lastname: Vukicevic
authors.forename: H
authors.initials: H
authors.lastname: Stopper
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003069:Coffee / Q000737:chemistry*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D004338:Drug Combinations; D005434:Flow Cytometry; D005720:Gamma Rays; D007940:Leukemia L5178 / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D003069:Coffee; D004338:Drug Combinations; D009153:Mutagens; D010936:Plant Extracts; D020011:Protective Agents