Assaying gene function by growth competition experiment.
High-throughput screening and analysis is one of the emerging paradigms in biotechnology. In particular, high-throughput methods are essential in the field of functional genomics because of the vast amount of data generated in recent and ongoing genome sequencing efforts. In this report we discuss integrated functional analysis methodologies which incorporate both a growth competition component and a highly parallel assay used to quantify results of the growth competition. Several applications of the two most widely used technologies in the field, i.e., transposon mutagenesis and deletion strain library growth competition, and individual applications of several developing or less widely reported technologies are presented.
Merritt
Joshua
J
Department of Chemical Engineering, University of Delaware, Newark 19716, USA.
Edwards
Jeremy S
JS
D000818:Animals; D003163:Competitive Behavior / Q000502:physiology*; D016172:DNA Fingerprinting / Q000379:methods*; D017353:Gene Deletion; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D016254:Mutagenesis, Insertional / Q000379:methods*; D012641:Selection, Genetic