title: Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+.
abstract: Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)2(PVP)10]2+ [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)2(PVP)10]2+, and [Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
authors.affiliation: National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
authors.forename: Lynn
authors.initials: L
authors.lastname: Dennany
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Forster
authors.forename: Blanaid
authors.initials: B
authors.lastname: White
authors.forename: Malcolm
authors.initials: M
authors.lastname: Smyth
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000187:drug effects; D004563:Electrochemistry; D004852:Epoxy Compounds / Q000494:pharmacology; D008163:Luminescent Measurements; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D011145:Polyvinyls / Q000737:chemistry*
chemical_list: D004852:Epoxy Compounds; D009841:Oligonucleotides; D009942:Organometallic Compounds; D011145:Polyvinyls; C492099:bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))osmium(II); C492100:bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))ruthenium(II); D004247:DNA; C013690:styrene oxide