Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR.
An attempt was made to detect various viruses of Piper betle grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (Cucumber mosaic virus) and potyvirus (Bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. DAC-ELISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.
Raj
S K
SK
Molecular Virology, National Botanical Research Institute, Lucknow 226 001, India.
Srivastava
A
A
Chowdhury
M R
MR
Johri
J K
JK
D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D031721:Piper betle / Q000821:virology*; D017800:Potyvirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral