Nicotinic acetylcholine receptor alpha5 subunits modulate oxotremorine-induced salivation and tremor.
Neuronal nicotinic acetylcholine receptors (nAChRs) are composed of 12 subunits (alpha2-alpha10 and beta2-beta4). alpha5 Subunits, expressed throughout the central nervous system (CNS) and the autonomic nervous system (ANS), possess unique pharmacological properties. The effects of oxotremorine (OXO) on autonomic functions and tremor were examined in mice lacking alpha5 nAChR subunits (alpha5-/-) and compared with those in wild-type (WT) control mice. The alpha5-/- mice showed significantly increased salivation and tremor responses to OXO. The hypothermia, bradycardia and defecation induced by OXO were of similar magnitudes in the two mouse strains. The enhanced OXO effects in alpha5-/- mice indicate inhibitory effects of alpha5 subunits in autonomic ganglia, and support the participation of these subunits in cholinergic transmission in autonomic ganglia.
Wang
Ningshan
N
Department of Physiology and Pharmacology, Sackler Medical School, Tel Aviv University, Tel Aviv, Israel.
Orr-Urtreger
Avi
A
Chapman
Joab
J
Rabinowitz
Ruth
R
Korczyn
Amos D
AD
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001919:Bradycardia / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D003672:Defecation / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D005725:Ganglia, Autonomic / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D007035:Hypothermia / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009433:Neural Inhibition / Q000187:drug effects / Q000235:genetics; D010095:Oxotremorine / Q000494:pharmacology*; D021122:Protein Subunits / Q000172:deficiency / Q000187:drug effects* / Q000235:genetics; D011978:Receptors, Nicotinic / Q000172:deficiency / Q000187:drug effects* / Q000235:genetics; D012472:Salivation / Q000187:drug effects / Q000235:genetics; D009435:Synaptic Transmission / Q000187:drug effects / Q000235:genetics; D014202:Tremor / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism
D018721:Muscarinic Agonists; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D010095:Oxotremorine; D000109:Acetylcholine