title: Maturation of dendritic cells by bacterial immunomodulators.
abstract: Dendritic cells (DC) become fully functional upon maturation by various stimuli. We tested whether an immunostimulatory effect of clinically used immunomodulators (Luivac, Biostim, Ribomunyl, Imudon, Bronchovaxom) is caused by direct DC activation. We found that Luivac, Biostim and Ribomunyl have a very high DC stimulatory potential in vitro. The level of DC activation was comparable or higher than DC maturation induced by standard maturation stimuli, Poly (I:C) or lipopolysaccharide. Treated DC had activated phenotype, reduced phagocytic activity and they induced the proliferation of allogeneic T lymphocytes. These results are important for understanding the physiology of action of these widely prescribed agents. Administration of bacterial immunomodulators should be considered with care to avoid the potential risk of inducing an autoimmune disease. They could also be used as well-defined maturating agents in the protocols used for the ex vivo production of DC-based vaccines for clinical trials.
authors.affiliation: Institute of Immunology, 2nd Medical Faculty, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic. radek.spisek@lfmotol.cuni.cz
authors.forename: Radek
authors.initials: R
authors.lastname: Spisek
authors.forename: Jitka
authors.initials: J
authors.lastname: Brazova
authors.forename: Daniela
authors.initials: D
authors.lastname: Rozkova
authors.forename: Katerina
authors.initials: K
authors.lastname: Zapletalova
authors.forename: Anna
authors.initials: A
authors.lastname: Sediva
authors.forename: Jirina
authors.initials: J
authors.lastname: Bartunkova
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D000954:Antigens, Surface / Q000032:analysis; D001419:Bacteria / Q000737:chemistry*; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects*; D003911:Dextrans; D004705:Endocytosis; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D015203:Reproducibility of Results; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D003470:Culture Media; D016207:Cytokines; D003911:Dextrans; D016753:Interleukin-10; D018664:Interleukin-12; D016650:Fluorescein-5-isothiocyanate