title: The action of the insecticide imidacloprid on the respiratory rhythm of an insect: the beetle Tenebrio molitor.
abstract: Imidacloprid is an insecticide which has the nicotinic acetylcholine receptors (nAChRs) as its primary site of action; acetylcholine is the major excitatory neurotransmitter in the insect central nervous system (CNS). In this study, the action of imidacloprid was tested using the synapses of the respiratory central pattern generator of the beetle Tenebrio molitor. The no observed effect concentration (NOEC) for imidacloprid was estimated to be between 0.001 and 0.010 microM. A concentration of 0.10 microM caused hyperexcitation in firing of the respiratory motoneurons, while the concentration of 1.00 microM caused an abrupt increase in their frequency and then a complete inhibition of the activity of the respiratory motoneurons. The possible implication of the action of such low concentrations of imidacloprid in the contraction of the respiratory muscles is also demonstrated and discussed.
authors.affiliation: Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University, Thessaloniki 54124, Hellas, Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Zafeiridou
authors.forename: George
authors.initials: G
authors.lastname: Theophilidis
mesh_terms: D000200:Action Potentials; D000818:Animals; D017952:Ganglia, Invertebrate / Q000166:cytology; D007093:Imidazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D007306:Insecticides / Q000494:pharmacology*; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010507:Periodicity; D012119:Respiration / Q000187:drug effects; D013712:Tenebrio / Q000502:physiology*
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid