Properties of proteins in cancer procoagulant preparations that are detected by anti-tissue factor antibodies.
Cancer procoagulant (CP) and tissue factor (TF; only in complex with Factor VIIa (FVIIa)) can activate FX to FXa. Controversy still exists whether or not CP is an entity different from TF, or whether CP activity is due to contamination of CP preparations with TF/FVIIa complex. We therefore looked for proteins in CP preparations that were detected by anti-TF antibodies and then sequenced these proteins. One- and two-dimensional gels of CP and TF were used to identify proteins immunoreactive to monoclonal anti-CP and anti-TF antibodies (Mabs). Those proteins in the CP preparation recognized by anti-TF antibodies were sequenced. Angiotensinogen precursor, alpha-1-antitrypsin precursor, and vitamin D-binding protein were identified along with one so far unidentified sequence; however, no TF-sequences were identified. Also, no proteins with the correct molecular weight for TF were identified using anti-TF antibodies. It seems possible that CP preparations contain proteins that have some epitopes similar to the epitopes recognized in TF by anti-TF Mab. However, these proteins do neither have the molecular weight nor the amino acid sequence of TF.
Raasi
Shari
S
Division of Laboratory Research, Kantonsspital, St. Gallen, Switzerland.
Mielicki
Wojciech P
WP
Gordon
Stuart G
SG
Korte
Wolfgang
W
D000808:Angiotensinogen / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015153:Blotting, Western; D002845:Chromatography; D003029:Coagulants / Q000494:pharmacology*; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005170:Factor X / Q000737:chemistry; D006801:Humans; D009363:Neoplasm Proteins / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013925:Thromboplastin / Q000737:chemistry / Q000276:immunology*; D014809:Vitamin D-Binding Protein / Q000737:chemistry; D000515:alpha 1-Antitrypsin / Q000737:chemistry / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003029:Coagulants; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins; D014809:Vitamin D-Binding Protein; D000515:alpha 1-Antitrypsin; D000808:Angiotensinogen; D005170:Factor X; D013925:Thromboplastin; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant