Construction and application of a promoter-trapping vector with methyl parathion hydrolase gene mpd as the reporter.
A facilitative and efficient promoter-trapping vector, pUC-mpd, was constructed with the promoterless methyl parathion hydrolase gene as the reporter. This reporter gene is easily used to clone promoters with different promoting strength on selective plates. Promoter regions of the ytkA and ywoF genes with strong promoting and signal peptide functions were cloned from the Bacillus subtilis 168 genomic promoter library with this vector.
Cui
Zhong-Li
ZL
Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of the Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, P.R. China. czl@njau.edu.cn
Zhang
Xiao-Zhou
XZ
Zhang
Zhong-Hui
ZH
Li
Shun-Peng
SP
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000378:metabolism; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D019992:Databases as Topic; D004926:Escherichia coli / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D017930:Genes, Reporter; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D004247:DNA; C495424:methyl parathion hydrolase, Pseudomonas; D010744:Phosphoric Monoester Hydrolases