Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria.
Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil.
Schoenborn
Liesbeth
L
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia.
Yates
Penelope S
PS
Grinton
Bronwyn E
BE
Hugenholtz
Philip
P
Janssen
Peter H
PH
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D012988:Soil Microbiology
D003470:Culture Media