Analysis of protein-protein proximities using the split-ubiquitin system.
The split-ubiquitin system is a fragment complementation assay that is based on a conditional proteolysis design. The system has been used to study protein-protein interactions between membrane proteins and to screen for new interaction partners for transcription factors. This paper outlines the recent progress in the split-ubiquitin technology that has been made possible through the development of new reporter proteins.
Lehming
Norbert
N
Department of Microbiology, Faculty of Medicine, National University of Singapore. micln@nus.edu.sg
D006868:Hydrolysis; D011506:Proteins / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*
D011506:Proteins; D025801:Ubiquitin