title: Characterization of hydroxyl radical generation in the striatum of free-moving rats due to carbon monoxide poisoning, as determined by in vivo microdialysis.
abstract: Carbon monoxide (CO) poisoning caused by CO exposure at 3000 ppm for 40 min resulted in stimulation of hydroxyl radical (*OH) generation (estimated by measuring 2,3-dihydroxybenzoic acid (2,3-DHBA) production from salicylic acid) in the striatum of free-moving rats, as determined by means of brain microdialysis. Pretreatment with a voltage-dependent Na+ channel blocker, tetrodotoxin (TTX), lowered the basal level of 2,3-DHBA and strongly suppressed the increase in 2,3-DHBA induced by CO poisoning. CO poisoning significantly, though only slightly, increased extracellular glutamate in the striatum, and glutamate (Glu) receptor antagonists, such as MK-801 (dizocilpine) and NBQX, failed to suppress the CO-induced increase in 2,3-DHBA. These findings suggest that CO poisoning may induce Na+ influx via the voltage-dependent Na+ channels, resulting in stimulation of *OH generation in rat striatum. This effect may be independent of Glu receptor activation by increased extracellular Glu.
authors.affiliation: Department of Forensic Medicine, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160-8402, Japan. s-hara@tokyo-med.ac.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hara
authors.forename: Toshiji
authors.initials: T
authors.lastname: Mukai
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Kurosaki
authors.forename: Fumi
authors.initials: F
authors.lastname: Kuriiwa
authors.forename: Takahiko
authors.initials: T
authors.lastname: Endo
mesh_terms: D000818:Animals; D002249:Carbon Monoxide Poisoning / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013779:Tetrodotoxin / Q000494:pharmacology; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D062385:Hydroxybenzoates; D011810:Quinoxalines; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D017665:Hydroxyl Radical; D018698:Glutamic Acid; D013779:Tetrodotoxin; D016291:Dizocilpine Maleate; C009135:2,3-dihydroxybenzoic acid