title: Radiation processed amniotic membranes in the treatment of non-healing ulcers of different etiologies.
abstract: The amniotic membranes were collected from the placentae of selected and screened donors. Processing was done by washing the fresh amniotic membrane successively in sterile saline, 0.05% sodium hypochlorite solution and sterile distilled water until it was completely cleared of blood particles. The membranes were sterilized by gamma irradiation at 25 kGy. The processed amniotic membranes were applied to 50 open wounds comprising of 42 full thickness defects and eight partial thickness defects. These included leprotic, diabetic, traumatic, gravitational ulcers and superficial burn in the form of scald and corrosive burn. The radiation processed amniotic membranes favoured healing of unresponsive and non-healing ulcers of different etiologies. Ulcers with duration of minimum 3 weeks to maximum 12 months were found to heal in 2-6 weeks by the application of amniotic membranes.
authors.affiliation: Defence Laboratory, Jodhpur, India. ritaami@yahoo.com
authors.forename: Rita
authors.initials: R
authors.lastname: Singh
authors.forename: U S
authors.initials: US
authors.lastname: Chouhan
authors.forename: Sumita
authors.initials: S
authors.lastname: Purohit
authors.forename: Pallavi
authors.initials: P
authors.lastname: Gupta
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: M P
authors.initials: MP
authors.lastname: Chacharkar
authors.forename: Dilip
authors.initials: D
authors.lastname: Kachhawa
authors.forename: B C
authors.initials: BC
authors.lastname: Ghiya
mesh_terms: D000650:Amnion / Q000528:radiation effects; D001684:Biological Dressings; D002056:Burns / Q000473:pathology / Q000628:therapy; D005720:Gamma Rays; D006801:Humans; D012883:Skin Ulcer / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D013997:Time Factors; D014945:Wound Healing