title: Prevalence and distribution of HSV-1, VZV, and HHV-6 in human cranial nerve nuclei III, IV, VI, VII, and XII.
abstract: The etiology of idiopathic cranial nerve palsies often remains unresolved. It has been hypothesised that viral reactivation of herpesviruses in the corresponding nuclei in the brainstem is the cause. We investigated the distribution of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) in nuclei that are associated with peripheral sensory ganglia [oculomotor (nIII), facial (nVII) nuclei] and in nuclei that are not associated with peripheral sensory ganglia [trochlear (nIV), abducens (nVI), and hypoglossal (nXII) nuclei] of five human brainstems. Samples of the cranial nerve nuclei and adjacent control tissue were taken from histological sections after precise identification of every single nucleus and control tissue. DNA and RNA amplification methods were used to determine the prevalence and distribution of HSV-1 and VZV. The distribution of human herpes virus type 6 (HHV-6) was also determined and served as a control, since HHV-6 infection has never been associated with idiopathic cranial nerve palsies. HSV-1 was distributed at random in all cranial nerve nuclei and control tissue, whereas VZV DNA was not detected in any of the samples examined. Surprisingly, HHV-6 was present in almost all samples where HSV-1 was also present, however, the latency associated transcript (LAT) of HSV-1 was not found in any of the samples positive for HSV-1 DNA. The absence of LAT in the samples positive for HSV-1 and the distribution of HSV-1 and HHV-6 do not support the hypothesis that idiopathic cranial nerve palsies result from viral reactivation in the brainstem nuclei.
authors.affiliation: Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians University, Munich, Germany. dtheil@brain.nefo.uni-muenchen.de
authors.forename: Diethilde
authors.initials: D
authors.lastname: Theil
authors.forename: Anja K E
authors.initials: AK
authors.lastname: Horn
authors.forename: Tobias
authors.initials: T
authors.lastname: Derfuss
authors.forename: Michael
authors.initials: M
authors.lastname: Strupp
authors.forename: Viktor
authors.initials: V
authors.lastname: Arbusow
authors.forename: Thomas
authors.initials: T
authors.lastname: Brandt
mesh_terms: D000010:Abducens Nerve / Q000821:virology; D000328:Adult; D000368:Aged; D001933:Brain Stem / Q000821:virology*; D003389:Cranial Nerve Diseases / Q000209:etiology; D003391:Cranial Nerves / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005154:Facial Nerve / Q000821:virology; D005260:Female; D005724:Ganglia / Q000821:virology; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D014645:Herpesvirus 3, Human / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D007002:Hypoglossal Nerve / Q000821:virology; D008297:Male; D035683:MicroRNAs; D021141:Nucleic Acid Amplification Techniques; D009802:Oculomotor Nerve / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D014321:Trochlear Nerve / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; D012367:RNA, Viral; D014764:Viral Proteins; C477253:latency associated transcript, herpes simplex virus-1