title: Skeletal muscle morphology and capillarization of renal failure patients receiving different dialysis therapies.
abstract: The morphology of gastrocnemius muscles was examined in RFPs (renal failure patients) being treated using HD (haemodialysis) and CAPD (continuous ambulatory peritoneal dialysis). RFPs (n=24) volunteered to participate in the present study. Twelve RFPs (five women and seven men; mean age, 55 years) were undergoing CAPD treatment and 12 RFPs (two women and ten men; mean age, 62 years) were undergoing HD treatment. Muscle biopsies from gastrocnemius muscles were found not to differ (P>0.05) in fibre type distribution, MyHC (myosin heavy chain) expression or fibre CSA (cross-sectional area) between the two groups. There were, however, significant differences (P<0.05) in CC/F (capillary contact/fibre), C/F (capillary to fibre ratio) and cytochrome c oxidase activity. The HD group had 33% more CC/F, with a 19% higher C/F and 33% greater cytochrome c activity in glycolytic fibres (II) than the CAPD group. There were no apparent differences in age, gender, co-morbidity, self-reported physical activity or physical functioning between the two groups, which could account for the difference in muscle capillarity between the groups. The HD patients were, however, administered heparin as a routine part of the dialysis therapy. The possibility is discussed that heparin in combination with mild anaemia and acidosis may have augmented angiogenesis in the HD patients.
authors.affiliation: Centre for Biophysical and Clinical Research into Human Movement, Manchester Metropolitan University, Alsager ST7 2HL, UK. gsakkas@itsa.ucsf.edu
authors.forename: Giorgos K
authors.initials: GK
authors.lastname: Sakkas
authors.forename: Derek
authors.initials: D
authors.lastname: Ball
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Sargeant
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Mercer
authors.forename: Pelagia
authors.initials: P
authors.lastname: Koufaki
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Naish
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D002196:Capillaries / Q000473:pathology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000473:pathology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D018482:Muscle, Skeletal / Q000098:blood supply / Q000201:enzymology / Q000473:pathology*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D006435:Renal Dialysis / Q000379:methods*
chemical_list: D003576:Electron Transport Complex IV