Biomarkers in maternal and newborn blood indicate heightened fetal susceptibility to procarcinogenic DNA damage.
Polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]pyrene (BaP) are widespread air contaminants released by transportation vehicles, power generation, and other combustion sources. Experimental evidence indicates that the developing fetus is more susceptible than the adult to carcinogenic effects of PAHs, although laboratory studies in rodents suggest that the dose to fetal tissues is an order of magnitude lower than that to maternal tissues. To assess fetal versus adult susceptibility to PAHs and environmental tobacco smoke (ETS), we compared carcinogen-DNA adducts (a biomarker associated with increased cancer risk) and cotinine (a biomarker of tobacco smoke exposure) in paired blood samples collected from mothers and newborns in New York City. We enrolled 265 nonsmoker African-American and Latina mother-newborn pairs in New York City between 1997 and 2001 (estimated average ambient air BaP concentrations < 0.5 ng/m3). Despite the estimated 10-fold lower fetal dose, mean levels of BaP-DNA adducts as determined by high-performance liquid chromatography-fluorescence were comparable in paired New York City newborn and maternal samples (0.24 adducts per 10(8) nucleotides, 45% of newborns with detectable adducts vs. 0.22 per 10(8) nucleotides, 41% of mothers with detectable adducts). However, by the Wilcoxon signed-rank test, the levels in newborns were higher (p = 0.02). Mean cotinine was higher in newborns than in mothers (1.7 ng/mL, 47% detectable vs. 1.28 ng/mL, 44% detectable). Consistent with our prior study in a Caucasian Polish population, these results indicate increased susceptibility of the fetus to DNA damage and reduced ability to clear ETS constituents. The findings have implications for risk assessment, given the need to protect children as a sensitive subset of the population.
Perera
Frederica P
FP
Mailman School of Public Health of Columbia University, Department of Environmental Health Sciences, New York, New York 10032, USA. fpp1@columbia.edu
Tang
Deliang
D
Tu
Yi-Hsuan
YH
Cruz
Linda Ali
LA
Borjas
Mejico
M
Bernert
Tom
T
Whyatt
Robin M
RM
D000328:Adult; D001741:Black or African American; D001564:Benzo(a)pyrene / Q000506:poisoning; D015415:Biomarkers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D015331:Cohort Studies; D003367:Cotinine / Q000652:urine; D018736:DNA Adducts / Q000032:analysis*; D004249:DNA Damage; D004293:Dominican Republic / Q000208:ethnology; D005314:Embryonic and Fetal Development; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D018811:Maternal Exposure; D009153:Mutagens / Q000506:poisoning; D009519:New York City; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D011247:Pregnancy; D018570:Risk Assessment; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014505:Urban Population
D015415:Biomarkers; D018736:DNA Adducts; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D014028:Tobacco Smoke Pollution; D001564:Benzo(a)pyrene; D003367:Cotinine