title: Automated ribotyping, a rapid typing method for analysis of Erysipelothrix spp. strains.
abstract: Automated ribotyping classified 70 Erysipelothrix species strains, previously classified into 14 RAPD patterns and into 63 PFGE patterns, into 27 ribogroups. Twenty-three strains of the 70 analyzed and classified into 13 ribogroups were previously classified into six ribotypes by the traditional ribotyping method. Moreover, automated ribotyping differentiated seven strains that were not differentiated by PFGE. Therefore, automated ribotyping was more sensitive than RAPD and traditional ribotyping, and it might be a useful method for a rapid screening in epidemiological study of strains of this genus, and more accurate results can be obtained when this method is used together with PFGE.
authors.affiliation: Laboratory of Animal Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Tomomitsu Alexandre
authors.initials: TA
authors.lastname: Okatani
authors.forename: Motoko
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Yoshida
authors.forename: Michiko
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanno
authors.forename: Masuo
authors.initials: M
authors.lastname: Ogawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Horikita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Horisaka
authors.forename: Takahide
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashidani
mesh_terms: D001331:Automation / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004888:Erysipelothrix / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D021521:Ribotyping / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S