Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography.
Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata was conducted using high-speed counter-current chromatograph (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:6:1.5:7.5, v/v). In a single operation, 28 mg of 5,7-dihydroxy-3',4'-trihydroxyflavone-3-O-6''-rhamnose and 18 mg of 5,7-dihydroxy-3',4'-dihydroxyflavone-3-O-6''-rhamnose was obtained from 150 mg of the extract. The chemical structure of the two compounds was elucidated by electrospray ionization (EIS) MS and NMR.
Du
Qizhen
Q
Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. qizhendu@mail.hzic.edu.cn
Chen
Ping
P
Jerz
Gerold
G
Winterhalter
Peter
P
D032421:Ampelopsis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification*; D006027:Glycosides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts