title: Covalent display of oligosaccharide arrays in microtiter plates.
abstract: A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 muM).
authors.affiliation: Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Marian C
authors.initials: MC
authors.lastname: Bryan
authors.forename: Fabio
authors.initials: F
authors.lastname: Fazio
authors.forename: Hing-Ken
authors.initials: HK
authors.lastname: Lee
authors.forename: Cheng-Yuan
authors.initials: CY
authors.lastname: Huang
authors.forename: Aileen
authors.initials: A
authors.lastname: Chang
authors.forename: Michael D
authors.initials: MD
authors.lastname: Best
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Calarese
authors.forename: Ola
authors.initials: O
authors.lastname: Blixt
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.forename: Dennis
authors.initials: D
authors.lastname: Burton
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D002240:Carbohydrate Sequence; D020650:Combinatorial Chemistry Techniques; D004229:Dithiothreitol / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D014230:Triazoles; D004229:Dithiothreitol