title: A new 39-plex analysis method for SNPs including 15 blood group loci.
abstract: A novel 39-plex typing system for single nucleotide polymorphisms (SNPs) has been developed. This multiplex approach has the advantage of being able to type 38 autosomal SNPs and one sex-discriminating base exchange site on the X and Y chromosomes rapidly and simultaneously. The SNP loci on the autosomes, which we examined, contain 15 loci distributed on blood type genes: three on RhCE, two each on Km and Gc, and one each on Duffy, AcP1, Tf, MN, GPT, EsD, PI, and Kidd genes. Thirty-seven genomic DNA fragments containing a total of 38 SNPs and one sex-discriminating site were amplified in one multiplex PCR reaction. Following the reaction, single nucleotide primer extension reaction was performed by dividing these SNP loci into five groups. The SNP type of each of the 39 loci was determined at one time by capillary electrophoresis using the newly designed multi-injection method. The combined PD (power of discrimination) of this typing system was (1-1.1) x 10(-14), and the MEC (mean exclusion chance) was 0.9990. We applied this system to forensic cases, including 16 paternity testing cases (13 non-exclusion and three exclusion cases) and one personal identification case. For the paternity testing cases, the highest Essen-Möller's W-value was 0.9999995. The pM (matching probability) of the personal identification case was 2.22 x 10(-17). These data showed that this system was an excellent tool for use in forensic cases of paternity testing and personal identification.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine and Dentistry, Okayama University, 700-8558, Japan. inagaki@md.okayama-u.ac.jp
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Inagaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Doi
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Takata
authors.forename: Takaki
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Imabayashi
authors.forename: Kei
authors.initials: K
authors.lastname: Yoshitome
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyaishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Ishizu
mesh_terms: D001789:Blood Group Antigens / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
chemical_list: D001789:Blood Group Antigens; D017931:DNA Primers