Massively parallel signature sequencing (MPSS) as a tool for in-depth quantitative gene expression profiling in all organisms.
Massively parallel signature sequencing (MPSS) is one of the newest tools available for conducting in-depth expression profiling. MPSS is an open-ended platform that analyses the level of expression of virtually all genes in a sample by counting the number of individual mRNA molecules produced from each gene. There is no requirement that genes be identified and characterised prior to conducting an experiment. MPSS has a routine sensitivity at a level of a few molecules of mRNA per cell, and the datasets are in a digital format that simplifies the management and analysis of the data. Therefore, of the various microarray and non-microarray technologies currently available, MPSS provides many advantages for generating the type of complete datasets that will help to facilitate hypothesis-driven experiments in the era of digital biology.
Reinartz
Jeanette
J
Lynx Therapeutics, GmbH, Germany.
Bruyns
Eddy
E
Lin
Jing-Zhong
JZ
Burcham
Tim
T
Brenner
Sydney
S
Bowen
Ben
B
Kramer
Michael
M
Woychik
Rick
R
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
D018076:DNA, Complementary; D012333:RNA, Messenger