[Preparation and characterization of podophyllotoxin-dipalmitoylphosphatidylcholine proliposome].
OBJECTIVE
To prepare podophyllotoxin-dipalmitoylphosphatidylcholine (PPT-DPPC) proliposomes (PPT-DPPC-PL) for improvement of the stability of PPT-DPPC liposome.


METHODS
Freeze-drying method was used to prepare PPT-DPPC-PL, and the particle morphology, size range, encapsulation efficiency and stability of PPT-DPPC liposome were investigated.


RESULTS
After hydration of PPT-DPPC-PL, PPT-DPPC liposome appeared multivesicular under electron microscope and the particles were distributed homogeneously with an average particle size of 1.45+/-0.38 microm. The encapsulation efficiency of PPT was 72.3%, and after storage at 4 to 40 degrees Celsius; for 1 to 6 months, the proliposome remained stable.


CONCLUSIONS
The prepared PPT-DPPC-PL particles by freeze-drying method are evenly distributed. The preparation method is relatively simple with higher embedding ratio and better stability.
Lin
Zhong-fang
ZF
Department of Dermatology, Department of Pharmacy, First Military Medical University, Guangzhou 510515, China. achang3333@sohu.com
Zeng
Kang
K
Zhou
Zai-gao
ZG
Li
Guo-feng
GF
Xie
Fang-ming
FM
Zhu
Xiao-liang
XL
Zhang
San-quan
SQ
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000008:administration & dosage*; D004355:Drug Stability; D005612:Freeze Drying; D008081:Liposomes; D010316:Particle Size; D011034:Podophyllotoxin / Q000008:administration & dosage*; D013696:Temperature
D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; D011034:Podophyllotoxin