Lactic acid production with lactate dehydrogenase using the visible light sensitization of zinc porphyrin.
Lactic acid production with L-lactate dehydrogenase from Pig heart (LDH) and reduced methyl viologen produced by the visible light photosensitization of zinc tetrakis(4-methylpyridyl)porphyrin (ZnTMPyP) was investigated. When the sample solution containing triethanolamine as an electron-donating reagent, ZnTMPyP, methyl viologen as an electron carrier, pyruvic acid and LDH in potassium phosphate buffer (pH 7) was irradiated, lactic acid production was observed. After 240 min irradiation, the amount of lactic acid production and the yield of pyruvic acid to lactic acid were estimated to be 0.17 mmol dm(-3) and 17.0%, respectively.
Miyatani
Rie
R
Department of Applied Chemistry, Oita University, Dannoharu 700, Oita 870-1192, Japan.
Amao
Yutaka
Y
D000818:Animals; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D008665:Metalloporphyrins / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D010269:Paraquat / Q000378:metabolism; D018462:Photobiology; D017319:Photosensitizing Agents / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D034421:Sus scrofa
D008665:Metalloporphyrins; D017319:Photosensitizing Agents; D019344:Lactic Acid; C060088:zinc tetra(4-N-methylpyridyl)porphine; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D010269:Paraquat