Physical mapping of a rice lesion mimic gene, Spl1 , to a 70-kb segment of rice chromosome 12.
The rice lesion mimic mutant spotted leaf 1 ( spl1) was first identified in the rice ( Oryza sativa) cultivar Asahi in 1965. This mutant displayed spontaneous disease-like lesions in the absence of any pathogen, and was found to confer resistance to multiple isolates of rice blast. We employed a map-based cloning strategy to localize the Spl1 gene. A total of ten cleaved amplified polymorphic sequence (CAPS) markers linked to the Spl1 gene were identified and mapped to an 8.5-cM region on chromosome 12. A high-resolution genetic map was developed using these ten CAPS markers and a segregating population consisting of 3202 individuals. A BAC contig containing four BAC clones was constructed, and Spl1 was localized to a 423-kb region. Seven spl1 mutants were obtained from the IR64 deletion mutant collection, and molecular analysis using these mutants delimited the Spl1 gene to a 70-kb interval, covered by two BAC clones. These results provide the basis for cloning this gene, which is involved in cell death and disease resistance in rice.
Liu
G
G
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, P. R. China.
Wang
L
L
Zhou
Z
Z
Leung
H
H
Wang
G-L
GL
He
C
C
D005819:Genetic Markers; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic
D005819:Genetic Markers; D011506:Proteins; C088014:spore photoproduct lyase