title: In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris.
abstract: The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
authors.affiliation: Department of Chemical Engineering and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea. hjcha@postech.ac.kr
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
authors.forename: Nimish G
authors.initials: NG
authors.lastname: Dalal
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
mesh_terms: D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D010843:Pichia / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors
chemical_list: D007376:Interleukin-2; D049452:Green Fluorescent Proteins