Simple method for three-dimensional representation of 2-DE spots using a spreadsheet program.
Quantitative protein expression analysis based on two-dimensional gel electrophoresis requires comparison of spot volumes. We describe an algorithm to visualize two-dimensional spot patterns in three dimensions using a spreadsheet program for surface plotting.
Maurer
Martin H
MH
Department of Physiology and Pathophysiology, University of Heidelberg, Germany. maurer@uni-hd.de
D000465:Algorithms; D015180:Electrophoresis, Gel, Two-Dimensional; D007091:Image Processing, Computer-Assisted; D012984:Software