title: Boron binding with the quorum sensing signal AI-2 and analogues.
abstract: [reaction: see text] The unstable bacterial metabolic product, DPD, and the related natural product, laurencione, are shown to have a high affinity for borate complexation, through the hydrated analogue. The boron complex of DPD is Vibrio harveyi AI-2, an interspecies quorum sensing signal in bacteria, and an affinity column with a borate resin is effective in providing the first method for concentrating and purifying V. harveyi AI-2 from the biosynthetic product.
authors.affiliation: Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Semmelhack
authors.forename: Shawn R
authors.initials: SR
authors.lastname: Campagna
authors.forename: Charlotte
authors.initials: C
authors.lastname: Hwa
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Federle
authors.forename: Bonnie L
authors.initials: BL
authors.lastname: Bassler
mesh_terms: D001895:Boron / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010420:Pentanes / Q000737:chemistry*; D014733:Vibrio / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology
chemical_list: C493138:4,5-dihydroxy-2,3-pentanedione; D010420:Pentanes; D001895:Boron