Role of caspase in CD80 expression of superantigen-stimulated monocytes.
In this study, we demonstrated evidence for the induction of CD80+ monocytes by staphylococcal enterotoxin B (SEB) via caspase actions. Pre-treatment with pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of NF-kappaB, resulted in a significant reduction in the percentage of SEB- and interferon-gamma (IFN-gamma) (produced by SEB) -induced CD80+ monocytes. SEB and IFN-gamma activated NF-kappaB, which was inhibited by PDTC. SEB induced the activation of caspase-3 and -8, and pre-treatment with z-VAD-fmk, a broad-spectrum inhibitor of caspases, prevented the induction of apoptosis. Treating with z-VAD-fmk resulted in a reduction of the generation of CD80+ monocytes. These results indicated that CD80 driven by NF-kappaB is regulated by the enzymatic actions of caspases, which allows monocytes to participate in massive T-cell activation.
Shinohara
Fumiaki
F
Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai 980-8575, Japan.
Sato
Keiko
K
Suzuki
Maiko
M
Takada
Haruhiko
H
Echigo
Seishi
S
Rikiishi
Hidemi
H
D000328:Adult; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000737:chemistry; D017209:Apoptosis; D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000737:chemistry; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018089:Superantigens / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D013859:Thiocarbamates / Q000494:pharmacology; D015854:Up-Regulation
D000590:Amino Acid Chloromethyl Ketones; D000911:Antibodies, Monoclonal; D018122:B7-1 Antigen; D018076:DNA, Complementary; D004768:Enterotoxins; D004791:Enzyme Inhibitors; D016328:NF-kappa B; D011759:Pyrrolidines; D018089:Superantigens; D013859:Thiocarbamates; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C020972:pyrrolidine dithiocarbamic acid; C031818:enterotoxin B, staphylococcal; D012313:RNA; D007371:Interferon-gamma; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases