Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis.
Mastitis is a major cause of economic loss to the dairy industry. Lactoferrin (Lf) is known to contribute to resistance against bacterial infections. Hence, we decided to characterize the relevance between mastitis resistance and the variants of Lf gene. By using PCR-SSCP, five fragments within 5' region and all exons of bovine lactoferrin gene were amplified and identified the nucleotide diversity. For the five segments within the 5'-region: Lf5'-1, Lf5'-2, Lf5'-3, Lf5'-4, and Lf5'-5 from upstream to downstream, we found that three had base variation. Totally, mutations were observed in Lf5'-1, Lf5'-3, and Lf5'-5, exons 4, 8, 9, 11, 15, and intron 4. We analyzed the effects of all mutated loci on milk production traits with least squares method.
Li
Guo-Hua
GH
College of Animal Science and Technology, China Agricultural University, Beijing, PR China.
Zhang
Yuan
Y
Sun
Dong-Xiao
DX
Li
Ning
N
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D007781:Lactoferrin / Q000235:genetics*; D016018:Least-Squares Analysis; D008414:Mastitis, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment
D004247:DNA; D007781:Lactoferrin