Codon bias and heterologous protein expression.
The expression of functional proteins in heterologous hosts is a cornerstone of modern biotechnology. Unfortunately, proteins are often difficult to express outside their original context. They might contain codons that are rarely used in the desired host, come from organisms that use non-canonical code or contain expression-limiting regulatory elements within their coding sequence. Improvements in the speed and cost of gene synthesis have facilitated the complete redesign of entire gene sequences to maximize the likelihood of high protein expression. Redesign strategies are discussed here, including modification of translation initiation regions, alteration of mRNA structural elements and use of different codon biases.
Gustafsson
Claes
C
DNA 2.0, 1455 Adams Drive, Menlo Park, CA 94025, USA. cgustafsson@dnatwopointo.com
Govindarajan
Sridhar
S
Minshull
Jeremy
J
D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D003062:Codon; D011506:Proteins; D011994:Recombinant Proteins