title: An optimized CZE method for analysis of mono- and oligomeric aldose mixtures.
abstract: An optimized capillary electrophoresis (CZE) method to analyze complex mixtures of aldoses was developed. The approach allows simultaneous quantitative analysis of all four isomeric aldopentoses, eight aldohexoses, as well as xylo- and cellooligosaccharides up to the tetraoses. UV tagging with 4-aminobenzoic acid ethyl ester (ABEE) in combination with reductive amination was used as pre-column derivatization. With optimum baseline separation and short run times, the method is very robust, and especially suited to follow reaction and isomerization kinetics of monosaccharides.
authors.affiliation: Christian-Doppler-Laboratory, Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: John
authors.initials: J
authors.lastname: Sjöberg
authors.forename: Immanuel
authors.initials: I
authors.lastname: Adorjan
authors.forename: Thomas
authors.initials: T
authors.lastname: Rosenau
authors.forename: Paul
authors.initials: P
authors.lastname: Kosma
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D002236:Carbohydrate Conformation; D019075:Electrophoresis, Capillary / Q000379:methods; D006601:Hexoses / Q000737:chemistry; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D010429:Pentoses / Q000737:chemistry; D013997:Time Factors
chemical_list: D000447:Aldehydes; D006601:Hexoses; D009844:Oligosaccharides; D010429:Pentoses