6,7-Dihydroxy-3-phenylcoumarin inhibits thromboplastin induced disseminated intravascular coagulation.
6,7-Dihydroxy-3-phenylcoumarin (DHPC) was tested to determine whether it had any effect on vitamin K inhibition, by investigating the prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen level and platelet count. The anticoagulant and antithrombotic effects of DHPC were compared with those of warfarin by conducting a 4 h acute trial on thromboplastin-induced disseminated intravascular coagulation (DIC), investigating various haemostatic and antioxidant system parameters and performing a haemogram. Of most significance was that in the 5-d DHPC trial on healthy controls, PT, APTT, fibrinogen, platelet count remained within normal levels. In the 4-h DIC trial, both DHPC (0.025 mg/kg, i.v.) and warfarin (0.25 mg/kg, i.v.) significantly inhibited DIC, by reducing the PT, APTT, and fibrin degradation products and increasing fibrinogen levels and platelet count. In the DIC drug groups, lipid peroxidation significantly increased only in the warfarin group and glutathione significantly increased only in the DHPC group. However leucocyte count was significantly higher in the DHPC than the warfarin group. Further investigation is required for why DHPC is effective on the parameters investigated, at doses one-tenth of those of warfarin.
Tunali
Tugba
T
Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantasi, Turkey. ttunali@marmara.edu.tr
Yarat
Aysen
A
Bulut
Mustafa
M
Emekli
Nesrin
N
D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D003374:Coumarins / Q000627:therapeutic use*; D003864:Depression, Chemical; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000188:drug therapy*; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D015227:Lipid Peroxidation; D023421:Models, Animal; D010314:Partial Thromboplastin Time; D010976:Platelet Count; D011517:Prothrombin Time; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013925:Thromboplastin; D014859:Warfarin / Q000627:therapeutic use
C488830:6,7-dihydroxy-3-phenylcoumarin; D000925:Anticoagulants; D003374:Coumarins; D014859:Warfarin; D005340:Fibrinogen; D013925:Thromboplastin