title: Genotoxic potentiality of aqueous extract prepared from Chrysobalanus icaco L. leaves.
abstract: Plants have been related to our lives, being used as medicine, regardless of scientific evidence of side effects. This work analyses the toxicological effects of Chrysobalanus icaco L. aqueous extract, used in different pathologies. It was studied through: (i) alteration of plasmid pUC 9.1 topology; (ii) survival of bacterial strains submitted, or not, to previous treatment with SnCl2; (iii) transformation efficiency of E. coli strain by the treatment with the plasmid pUC 9.1. In (i), the treatment of the plasmid resulted in DNA single-strand breaks (SSB). A decrease of the lethal effect induced by SnCl2 in presence of the extract was found, while no C. icaco bacterial survival reduction was observed. The transformation efficiency of the plasmid was also reduced. Results suggest that the extract could present a potential genotoxic effect, as demonstrated either by the induction of SSB in plasmid or in transformation efficiency experiments. Finally, it presents an antioxidant action.
authors.affiliation: Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Av. 28 de setembro, 87, Rio de Janeiro 20551-030, Brazil.
authors.forename: S C
authors.initials: SC
authors.lastname: Ferreira-Machado
authors.forename: M P
authors.initials: MP
authors.lastname: Rodrigues
authors.forename: A P M
authors.initials: AP
authors.lastname: Nunes
authors.forename: F J S
authors.initials: FJ
authors.lastname: Dantas
authors.forename: J C P
authors.initials: JC
authors.lastname: De Mattos
authors.forename: C R
authors.initials: CR
authors.lastname: Silva
authors.forename: E G
authors.initials: EG
authors.lastname: Moura
authors.forename: R J A C
authors.initials: RJ
authors.lastname: Bezerra
authors.forename: A
authors.initials: A
authors.lastname: Caldeira-de-Araujo
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D045469:Chrysobalanaceae; D004249:DNA Damage; D004926:Escherichia coli / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity*; D018515:Plant Leaves; D010957:Plasmids / Q000187:drug effects; D014169:Transformation, Bacterial / Q000187:drug effects
chemical_list: D000975:Antioxidants; D010936:Plant Extracts