pO2 and regional blood flow in a rabbit model of limb ischemia.
Oxygen tension (pO2) in muscles and regional blood flow were measured in a rabbit model of limb ischemia. pO2 was measured repetitively by EPR oximetry with EMS char in four different muscle groups in the same animals. Blood flow in the same muscles at several time points was measured using microspheres. A linear mixed effects model was developed to analyze the data on pO2 and blood flow. The results suggest that while under normal conditions pO2 in muscles does not depend significantly on blood flow, immediately after arterial occlusion pO2 correlates linearly with blood flow. Within two weeks of occlusion the pO2 is recovered to 45% of baseline. This study demonstrates, for the first time, the applicability of EPR oximetry in animals larger than rodents.
Grinberg
Oleg Y
OY
EPR Center for the Study of Viable Systems, Dartmouth Medical School, Hanover, NH, USA. oleg.grinberg@dartmouth.edu
Hou
Huagang
H
Grinberg
Stalina A
SA
Moodie
Karen L
KL
Demidenko
Eugene
E
Friedman
Bruce J
BJ
Post
Mark J
MJ
Swartz
Harold M
HM
D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D006614:Hindlimb / Q000098:blood supply / Q000503:physiopathology; D007511:Ischemia / Q000097:blood* / Q000503:physiopathology*; D008297:Male; D008863:Microspheres; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism*; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000097:blood*; D011817:Rabbits; D011865:Radioisotope Dilution Technique; D012039:Regional Blood Flow; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010100:Oxygen