title: Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA.
abstract: Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.
authors.affiliation: Department of Biology, East China Normal University, Shanghai 200062, PR China. lqchen@bio.ecnu.edu.cn
authors.forename: Liqiao
authors.initials: L
authors.lastname: Chen
authors.forename: Hongbo
authors.initials: H
authors.lastname: Jiang
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Glenn Y
authors.initials: GY
authors.lastname: Deng
authors.forename: Zhanjiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D010053:Ovary / Q000378:metabolism*; D011817:Rabbits; D047048:Vitellins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D047048:Vitellins