Gliadin effect on fluctuation properties of phospholipid giant vesicles.
Gliadin is a fraction of wheat gluten, a protein supramolecular complex known for its remarkable and biotechnologically relevant viscoelastic properties. Very high molecular mass characterise these systems, thus hindering high-resolution structural investigations. It is known, however, that these proteins comprise rather extended, extensively interassociated structures, which respond for their peculiar mechanical behaviour. Besides these properties, some of gluten's fractions, such as gliadin, are also known to be involved in a nutritionally relevant pathology of auto-immune character, the celiac disease, supposedly related to some unusual structural features of the protein. Despite its medical relevance, however, the role played by gliadin in the etiology of the celiac disease is not sufficiently understood to date. In this work, we investigated the role of gliadin on mechanical properties of a membrane model of dioleoylphosphatidylcholine (DOPC) giant unilamellar vesicles. The technique of micropipette aspiration, coupled to videomicroscopy, was applied. The microvesicles, produced by electric field pulsing over metal-covered plates, were suctioned into the micropipettes under varying applied pressures. A significant increase in the values of the bilayer curvature constant, k(c), was observed, with a saturation effect being verified at around 0.02-0.03 gliadin/DOPC mass ratio, indicating that the membrane becomes less elastic in the presence of the protein. Possible correlations between the observed membrane fluctuation properties and the celiac disease etiology are suggested and discussed.
Monteiro
Angela M F
AM
Departmento de Quimica Fundamental, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970 Sao Paulo, SP, Brazil.
Arêas
Elizabeth P G
EP
Schröder
André
A
Fa
Nathalie
N
D055592:Biophysical Phenomena; D001703:Biophysics; D002446:Celiac Disease / Q000378:metabolism; D005903:Gliadin / Q000737:chemistry*; D005983:Glutens / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry; D018715:Microscopy, Video; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011312:Pressure; D014908:Triticum / Q000378:metabolism
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; D005983:Glutens; D005903:Gliadin; C017251:1,2-oleoylphosphatidylcholine