title: Identification of an alternative splicing transcript for the resistin gene and distribution of its mRNA in human tissue.
abstract: OBJECTIVE
  Adipocytes secrete a number of molecules such as tumor necrosis factor-alpha, leptin and free fatty acids that can influence the ability of the body to metabolize glucose. Recently, a novel 12.5 kDa cysteine-rich protein, termed resistin, was shown to be secreted by adipocytes. Resistin expression was markedly induced during the conversion of 3T3-L1 cells to mature adipocytes. Expression of resistin has been studied in human, mouse and rat; however, sequence information about an alternative splicing variant (ASV) of resistin mRNA has not been reported. In the present study, we investigated the occurrence of a novel ASV of the resistin gene in human normal tissues.
  
  
  METHODS
  We identified a novel ASV of resistin mRNA in human lung tissue by RT-PCR analysis in human lung tissue. We then investigated a novel ASV of resistin mRNA by real-time PCR analysis in 26 different types of normal human tissues.
  
  
  CONCLUSIONS
  We identified a novel deletion variant of the resistin transcript in the normal human tissues. The deleted transcript of resistin was characterized by an in-frame deletion of 78 bp, corresponding to the complete loss of exon 2 (resistin delta2 ASV). Thus, resistin delta2 ASV causes protein truncation. Our results provide the basis for more detailed studies on the regulation of resistin activity, and should assist in the development of clinical trials with resistin for the central regulation of adipogenesis and adipocyte metabolism.
authors.affiliation: Department of Obstetrics and Gynecology, Hachioji Medical Center of Tokyo Medical University, Tokyo 193-0998, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Nohira
authors.forename: Kumi
authors.initials: K
authors.lastname: Nagao
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Kameyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakai
authors.forename: Noritaka
authors.initials: N
authors.lastname: Fukumine
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Okabe
authors.forename: Soichi
authors.initials: S
authors.lastname: Kitano
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hisatomi
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D017398:Alternative Splicing; D005091:Exons; D017353:Gene Deletion; D006729:Hormones, Ectopic / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000502:physiology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D052243:Resistin
chemical_list: D006729:Hormones, Ectopic; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin