[FTIR spectroscopy studies on the apoptosis-promoting effect of TFAR19 on the erythroleukemia cell line MEL].
The changes in the cellular main components of the mouse erythroleukemia cell line MEL for TFAR19 gene transfection were studied by the technology of Fourier transform infrared spectroscopy (FTIR). Using the method of gene transfection with liposome, we obtained MEL-TF19 cell line, which stably carries TFAR19, a novel apoptosis-related gene. The expression of the gene on mRNA level was confirmed by RT-PCR. Then, FTIR spectra of the cells were measured in the course of apoptosis induced by serum deprivation. Our results indicated that after being transfected with TFAR19 gene, MEL-TF19 cells exhibited relatively higher protein content, higher transcriptional activity, and relatively lower phospholipid content as compared with those exhibited by MEL cells. All the above changes reflect the apoptosis-promoting effect of TFAR19 gene, and maybe account for the cellular rheological changes after TFAR19 gene transfection, which were discovered in our previous study.
Gu
Li
L
School of Life Science of Shandong University, Jinan 250150, China.
Xie
Lide
L
Yao
Weijuan
W
Ka
Weibo
W
Sun
Dagong
D
Wen
Zongyao
Z
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D016147:Genes, Tumor Suppressor; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C486488:Pdcd5 protein, mouse