title: Listeria monocytogenes isolated from cold-smoked fish products in Osaka City, Japan.
abstract: Listeria monocytogenes contamination of ready-to-eat seafood products commercially available in Osaka was examined between 1999 and 2000. L. monocytogenes was isolated from 12 (13%) of the 95 products tested. All positive samples were from cold-smoked fish with 9 being obtained during the summer. Thirteen isolates of L. monocytogenes were typed by pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR)-based typing methods. Isolates of the same serotype originating from the same manufacturer gave similar DNA profiles, irrespective of the type of sample or date of isolation. The finding suggest that persistent strains in each manufacturing facility proliferate during the summer and contaminate products during manufacturing processes.
authors.affiliation: Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138, Osaka 558-8585, Japan. h.nakamura@iphes.city.osaka.jp
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: K
authors.initials: K
authors.lastname: Ochi
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: J
authors.initials: J
authors.lastname: Ogasawara
authors.forename: A
authors.initials: A
authors.lastname: Hase
authors.forename: T
authors.initials: T
authors.lastname: Kitase
authors.forename: K
authors.initials: K
authors.lastname: Haruki
authors.forename: Y
authors.initials: Y
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004866:Equipment Contamination; D005396:Fish Products / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis*; D005525:Food-Processing Industry; D007564:Japan; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012621:Seasons
chemical_list: D004269:DNA, Bacterial