hIscA: a protein implicated in the biogenesis of iron-sulfur clusters.
This article describes the gene called hIscA, its transcription product and protein (hIscA) and its putative function. We screened a human brain cDNA expression library with serum from a patient suffering from the autoimmune Sjögren's syndrome (S5:823/94). One cDNA of 1.6-kbp clone was isolated. This clone contains the entire coding sequence for a protein unknown in human. IscA is ubiquitously expressed and expression levels vary among tissues. The 15.5-kDa predicted protein contains a structural domain named HESB, is located in the mitochondria and is implicated in the biogenesis of iron-sulfur clusters. Since this unknown protein is related to IscA-like protein, we suggest as name for this protein hIscA. The recombinant protein is recognized by a rabbit polyclonal antiserum generated against the carboxyl extreme of the Saccharomyces cerevisiae orthologue Isa1. In this article, we demonstrate the functional homology between hIscA and Isa1 proteins using Isa1 null mutant S. cerevisiae transformed with hIscA in a yeast functional complementation test. We also describe the rat homologue to this gene.
Cózar-Castellano
Irene
I
Laboratorio de Biología del Desarrollo, Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain.
del Valle Machargo
María
M
Trujillo
Elisa
E
Arteaga
María Francisca
MF
González
Tomás
T
Martín-Vasallo
Pablo
P
Avila
Julio
J
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D015723:Gene Library; D005816:Genetic Complementation Test; D006801:Humans; D007118:Immunoassay; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008969:Molecular Sequence Data; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017385:Sequence Homology; D012859:Sjogren's Syndrome / Q000097:blood* / Q000276:immunology
C489112:ISCA1 protein, human; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins