Cloning and tissue distribution of the chicken type 2 corticotropin-releasing hormone receptor.
We report the cloning of the complete coding sequence of the putative chicken type 2 corticotropin-releasing hormone receptor (CRH-R2) by rapid amplification of cDNA ends (RACE). The chicken CRH-R2 is a 412-amino acid 7-transmembrane G protein-coupled receptor, showing 87% identity to the Xenopus laevis and Oncorhynchus keta CRH-R2s, and 78-80% to mammalian CRH-R2s. The distribution of CRH-R2 mRNA was studied by RT-PCR analysis and compared to CRH-R1 distribution. Both CRH-R1 and CRH-R2 mRNA are expressed in the main chicken brain parts. In peripheral organs, CRH-R1 mRNA shows a more restricted distribution, whereas CRH-R2 mRNA is expressed in every tissue investigated, indicating that a number of actions of CRH and/or CRH-like peptides remain to be discovered in the chicken as well as in other vertebrates.
de Groef
Bert
B
Laboratory of Comparative Endocrinology, Catholic University of Leuven, B3000 Leuven, Belgium. bert.degroef@bio.kuleuven.ac.be
Grommen
Sylvia V H
SV
Mertens
Inge
I
Schoofs
Liliane
L
Kühn
Eduard R
ER
Darras
Veerle M
VM
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D002645:Chickens / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018019:Receptors, Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone