Spatial coordination of cytokinetic events by compartmentalization of the cell cortex.
During cytokinesis, furrow ingression and plasma membrane fission irreversibly separate daughter cells. How actomyosin ring assembly and contraction, vesicle fusion, and abscission are spatially coordinated was unknown. We found that during cytokinesis septin rings, located on both sides of the actomyosin ring, acted as barriers to compartmentalize the cortex around the cleavage site. Compartmentalization maintained diffusible cortical factors, such as the exocyst and the polarizome, to the site of cleavage. In turn, such factors were required for actomyosin ring function and membrane abscission. Thus, a specialized cortical compartment ensures the spatial coordination of cytokinetic events.
Dobbelaere
Jeroen
J
Institute of Biochemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, 8093 Zürich, Switzerland.
Barral
Yves
Y
D000205:Actomyosin / Q000502:physiology; D002451:Cell Compartmentation; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002687:Chitin Synthase / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008840:Microfilament Proteins / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D051304:Profilins; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D020703:ras GTPase-Activating Proteins
C099504:CDC12 protein, S cerevisiae; C086662:CDC3 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C101203:IQ motif containing GTPase activating protein 1; D008164:Luminescent Proteins; C112936:MYO1 protein, S cerevisiae; D008840:Microfilament Proteins; D051304:Profilins; C103130:SEC3 protein, S cerevisiae; C065889:SPA2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D020703:ras GTPase-Activating Proteins; D049452:Green Fluorescent Proteins; D000205:Actomyosin; D002687:Chitin Synthase; C400821:chitin synthase 2; D018995:Myosin Heavy Chains