Increase in gammadelta T cells in the blood of cattle persistently infected with bovine leukemia virus following administration of recombinant bovine IFN-gamma.
To examine the effect of recombinant bovine interferon-gamma (rbIFN-gamma) on cattle persistently infected with bovine leukemia virus (BLV), BLV-infected cattle were inoculated intraperitoneally with IFN-gamma. All cattle were febrile after inoculation with IFN-gamma and then recovered within 48 h. Flow cytometric analysis showed that the numbers of CD4+ and CD8+ T cells were decreased for 2-3 days and then their numbers were recovered. The number of gammadelta T cells increased after the fever. In contrast, the number of IgM+ lymphocytes remained low for about 1 week. Moreover, the numbers of syncytia produced by peripheral blood lymphocytes decreased and remained low compared to that before IFN-gamma administration. These results suggest that IFN-gamma induces the up-regulation of gammadelta T cells, decreases the number of IgM+ lymphocytes and suppresses the growth of BLV in BLV-infected cattle in vivo.
Murakami
Kenji
K
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. muraken@affrc.go.jp
Sentsui
Hiroshi
H
Inoshima
Yasuo
Y
Inumaru
Shigeki
S
D000818:Animals; D002353:Carrier State / Q000276:immunology; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000188:drug therapy* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D015726:Giant Cells / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D016130:Immunophenotyping / Q000662:veterinary; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D007958:Leukocyte Count / Q000662:veterinary; D018655:Lymphocyte Count / Q000662:veterinary; D008297:Male; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D007075:Immunoglobulin M; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; D007371:Interferon-gamma