Characterization of distinct conventional and plasmacytoid dendritic cell-committed precursors in murine bone marrow.
The developmental pathways and differentiation relationship of dendritic cell (DC) subsets remain unclear. We report that murine CD11c(+)MHC II(-) bone marrow cells, which are immediate DC precursors of CD8 alpha(+), CD8 alpha(-), and B220(+) DC in vivo, can be separated into B220(+) and B220(-) DC precursor subpopulations. Purified B220(-) DC precursors expand, and generate exclusively mature CD11c(+)CD11b(+)B220(-) DC in vitro and after adoptive transfer. B220(+) DC precursors, which resemble plasmacytoid pre-DC, have a lower proliferative potential than B220(-) DC precursors and generate both CD11b(-) B220(+) and CD11b(+)B220(-) DC populations. Both DC precursor populations can give rise to CD8 alpha(+) and CD8 alpha(-) DC subtypes. Our findings indicate that CD11c(+)MHC II(-)B220(+) and CD11c(+)MHC II(-)B220(-) bone marrow cells are distinct DC lineage-restricted precursors.
Diao
Jun
J
Multiorgan Transplantation Program, Toronto General Hospital and Department of Surgey, University of Toronto, Ontario, Canada.
Winter
Erin
E
Chen
Wenhao
W
Cantin
Claude
C
Cattral
Mark S
MS
D019264:Adoptive Transfer; D000818:Animals; D020296:Animals, Congenic; D001854:Bone Marrow Cells / Q000166:cytology*; D039521:CD11c Antigen / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D018899:CpG Islands / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013047:Specific Pathogen-Free Organisms
D039521:CD11c Antigen; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D017493:Leukocyte Common Antigens