Effect of low frequency ultrasonic stimulation on the secretion of siboflavin produced by Ecemothecium Ashbyii.
The structure of the cell membrane of Ecemothecium ashbyii is considered to be one of the main obstacles in the improvement of riboflavin productivity, which impedes the release of riboflavin from the cell into the fermentation broth. The results of the present study show that stimulation on growth and riboflavin biosynthesis phase, the content of riboflavin was most obviously enhanced, as compared with the control and the total riboflavin quantity released by ultrasonic treatment. The optimal stimulation time was from 104 to 112 h and ultrasonic should be loaded every 1.5 h.
Chuanyun
Dai
D
Key Laboratory of Biomechanics & Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China.
Bochu
Wang
W
Huan
Zhou
Z
Conglin
He
H
Chuanren
Duan
D
Wangqian
Liu
L
Toyama
Y
Y
Sakanishi
A
A
D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation; D012256:Riboflavin / Q000737:chemistry* / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
D003470:Culture Media; D012256:Riboflavin