[Effect of LHRH crasis protein as vaccine on the reproductive function of male rats].
OBJECTIVE
To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and male reproductive function.


METHODS
Trx-LHRH produced in vitro with a new crasis gene which crasised Trx gene and GnRH gene together, was used as vaccine, and hydroalaminum base as adjuvant, in adult SD rats. After 5 weeks of the first treatment, the same dosage was used again to enhance the effect of vaccine. Antibody level was measured by ELISA, and androgen level by RIA.


RESULTS
Trx-LHRH induced successfully the polycolonal antibody at the level of 1 :1 280 approximately 2 560 after 4 weeks of the first treatment, and 1 : 2 000 after 6 weeks of the enhanced treatment. Testosterone level was reduced significantly (P < 0.01) by ELISA, but there was reasonable variation among individuals. Sperm count was also reduced by Trx-LHRH treatment.


CONCLUSIONS
Trx-LHRH can be used as effective vaccine to induce antibody production, and at the same time, restrain the function of hypothatamas-pituitary-testis axis in vivo.
Cai
Ruifen
R
Jiangsu Family Planning Institute, Nanjing, Jiangsu 210029, China.
Pan
Qinqin
Q
Pan
Yong
Y
Jia
Yue
Y
Ma
Dingzhi
D
Wang
Xinghai
X
Cui
Yugui
Y
D000818:Animals; D000906:Antibodies / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013076:Sperm Count; D013739:Testosterone / Q000097:blood; D013879:Thioredoxins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000906:Antibodies; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D013879:Thioredoxins