title: Temperature dependence of ultraweak photon emission in fibroblastic differentiation after irradiation with artificial sunlight.
abstract: Yield of ultraweak photon emission in a cell culture model for biophotonic measurements using fibroblastic differentiation depended on the temperature of photonic measurement. The ultraweak photon emission of medium was significantly higher at 37 degrees C than at 25 degrees C and after UVB-irradiation this difference was even more pronounced. While with cells in the medium no temperature dependence could be determined in unirradiated samples, after UVB-irradiation of cells an increase of biophotonic emission was observed in postmitotic fibroblasts. While after several UVB exposures normal cells begin to absorb the ultraviolet light, cells from patients with the disease Xeroderma Pigmentosum loose this capacity. In view that fibroblasts play an essential role in skin aging, skin carcinogenesis and wound healing, the biophotonic model using the fibroblastic differentiation system provides to be a new and powerful non-invasive tool for the development of skin science.
authors.affiliation: BioFoton AG, P.O. Box 28, CH-1731 Ependes, Switzerland. biofoton@swissonline.ch
authors.forename: Hugo J
authors.initials: HJ
authors.lastname: Niggli
mesh_terms: D002454:Cell Differentiation / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D017785:Photons; D013472:Sunlight; D013696:Temperature