Identification and quantitation of G-protein alpha-subunits.
The demonstration that many intracellular signaling processes are mediated by a family of closely related guanine nucleotide binding proteins (G-proteins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involved.
Mullaney
Ian
I
Molecular Pharmacology Group, University of Glasgow, Scotland, United Kingdom.
Milligan
Graeme
G
D000906:Antibodies / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D044385:GTP-Binding Protein alpha Subunits / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D010455:Peptides / Q000276:immunology; D011233:Precipitin Tests / Q000379:methods
D000906:Antibodies; D044385:GTP-Binding Protein alpha Subunits; D010455:Peptides