title: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential.
abstract: Here a new, intrinsically pluripotent, CD45-negative population from human cord blood, termed unrestricted somatic stem cells (USSCs) is described. This rare population grows adherently and can be expanded to 10(15) cells without losing pluripotency. In vitro USSCs showed homogeneous differentiation into osteoblasts, chondroblasts, adipocytes, and hematopoietic and neural cells including astrocytes and neurons that express neurofilament, sodium channel protein, and various neurotransmitter phenotypes. Stereotactic implantation of USSCs into intact adult rat brain revealed that human Tau-positive cells persisted for up to 3 mo and showed migratory activity and a typical neuron-like morphology. In vivo differentiation of USSCs along mesodermal and endodermal pathways was demonstrated in animal models. Bony reconstitution was observed after transplantation of USSC-loaded calcium phosphate cylinders in nude rat femurs. Chondrogenesis occurred after transplanting cell-loaded gelfoam sponges into nude mice. Transplantation of USSCs in a noninjury model, the preimmune fetal sheep, resulted in up to 5% human hematopoietic engraftment. More than 20% albumin-producing human parenchymal hepatic cells with absence of cell fusion and substantial numbers of human cardiomyocytes in both atria and ventricles of the sheep heart were detected many months after USSC transplantation. No tumor formation was observed in any of these animals.
authors.affiliation: Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, Moorenstrasse 5, 40225 Düsseldorf, Germany. koegler@itz.uni-duesseldorf.de
authors.forename: Gesine
authors.initials: G
authors.lastname: Kögler
authors.forename: Sandra
authors.initials: S
authors.lastname: Sensken
authors.forename: Judith A
authors.initials: JA
authors.lastname: Airey
authors.forename: Thorsten
authors.initials: T
authors.lastname: Trapp
authors.forename: Markus
authors.initials: M
authors.lastname: Müschen
authors.forename: Niklas
authors.initials: N
authors.lastname: Feldhahn
authors.forename: Stefanie
authors.initials: S
authors.lastname: Liedtke
authors.forename: Rüdiger V
authors.initials: RV
authors.lastname: Sorg
authors.forename: Johannes
authors.initials: J
authors.lastname: Fischer
authors.forename: Claudia
authors.initials: C
authors.lastname: Rosenbaum
authors.forename: Susanne
authors.initials: S
authors.lastname: Greschat
authors.forename: Andreas
authors.initials: A
authors.lastname: Knipper
authors.forename: Jörg
authors.initials: J
authors.lastname: Bender
authors.forename: Ozer
authors.initials: O
authors.lastname: Degistirici
authors.forename: Jizong
authors.initials: J
authors.lastname: Gao
authors.forename: Arnold I
authors.initials: AI
authors.lastname: Caplan
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Colletti
authors.forename: Graça
authors.initials: G
authors.lastname: Almeida-Porada
authors.forename: Hans W
authors.initials: HW
authors.lastname: Müller
authors.forename: Esmail
authors.initials: E
authors.lastname: Zanjani
authors.forename: Peter
authors.initials: P
authors.lastname: Wernet
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000418:Albumins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D017690:Cell Transplantation; D036101:Cord Blood Stem Cell Transplantation; D005269:Femur / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000378:metabolism; D018377:Neurotransmitter Agents; D010006:Osteoblasts / Q000378:metabolism; D010641:Phenotype; D010920:Placenta / Q000098:blood supply*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D014471:Umbilical Veins
chemical_list: D000418:Albumins; D018377:Neurotransmitter Agents; D017493:Leukocyte Common Antigens