Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis.
Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one "Spironema culicis" strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography column in conjunction with Microbial Identification System Sherlock (MIDI Inc., Newark, DE, USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B. burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool for preliminary identification of spirochetes isolated from ticks and dipterans.
Cechová
Leona
L
Department of Microbiology, Faculty of Science, Masaryk University Brno, Tvrdého 14, 60200 Brno, Czech Republic. lejka@sci.muni.cz
Durnová
Eva
E
Sikutová
Silvie
S
Halouzka
Jirí
J
Nemec
Miroslav
M
D000818:Animals; D001181:Arthropods / Q000382:microbiology*; D002849:Chromatography, Gas; D018153:Czech Republic; D005227:Fatty Acids / Q000032:analysis*; D013144:Spirochaetales / Q000302:isolation & purification*
D005227:Fatty Acids