[Immunological reaction between the peptides from S1 domain of SARS coronavirus S-protein and the serum from SARS patients].
OBJECTIVE
To examine the immunological reactions between the peptides of SARS coronavirus (SARS-Cov) S-protein and the serum of SARS patients for identifying the SARS-Cov epitope.


METHODS
The peptides from S1 domain of SARS-Cov S-protein were synthesized by peptide synthesizer, and the immunological reaction of the peptides with the serum of SARS patients were examined by means of ELISA.


RESULTS
The optical density value of the immunological reaction of synthesized 8 peptides with SARS patient serum was 1.5-2.6 times higher than that with normal serum.


CONCLUSIONS
The 8 peptides from S1 domain of S protein appear to have low immunogenicity to the serum of SARS patients, indicating that these peptides may not be the epitope of the SARS-Cov.
Zhang
Jia-jie
JJ
Institute of Pharmaceutics Science, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. jydyys@fimmu.com
Wu
Shao-yu
SY
Xu
Wei
W
Hou
Jin-lin
JL
Wang
Zhan-hui
ZH
Jiang
Shi-bo
SB
Wu
Shu-guang
SG
D000595:Amino Acid Sequence; D000939:Epitopes; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000097:blood*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000276:immunology*
D000939:Epitopes; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus