Real-time imaging of T-cell development.
The cellular and molecular dynamics underlying intrathymic T-cell development are still poorly understood. Dynamic imaging techniques offer new opportunities for dissecting the complex journey of developing T cells in the thymus. In particular, two-photon laser scanning microscopy has recently demonstrated great promise in tracking lymphocyte behavior in tissue environments. Resolving thymocyte behavior at the single-cell level should help to clarify the mode of trafficking in the thymus and identify the spatio-temporal aspects of thymic selection events.
Bousso
Philippe
P
Institut National de la Sante et de la Recherche Medicale U277, Département d'Immunologie, Institut Pasteur, 25 Rue du Dr Roux, 75015 Paris, France. bousso@pasteur.fr
D000818:Animals; D002454:Cell Differentiation / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D051379:Mice; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology