title: Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen.
abstract: OBJECTIVE
  To evaluate the effects of animal milk containing fucosylated antigens on Helicobacter pylori (H. pylori) binding to Lewis b antigen.
  
  
  METHODS
  A mammary gland expression vector containing human alpha1-3/4-fucosyltransferase cDNA sequences was constructed. Transient expression of human alpha1-3/4-fucosyltransferase cDNA in goat mammary cell and establishment of transgenic mice were performed. The adhesion inhibitory properties of milk samples were analyzed by using H. pylori.
  
  
  RESULTS
  Goat milk samples were found to inhibit bacterial binding to Lewis b antigen. The highest inhibition was observed 42 h after injection of the plasmid. The binding activity of H. pylori to Lewis b antigen reduced mostly, by 83%, however milk samples from transgenic mice did not inhibit H. pylori binding to Lewis b antigen.
  
  
  CONCLUSIONS
  The use of "humanized" animal milk produced by the transgenic introduction of fucosylated antigen can perhaps provide an alternative therapy and preventive measure for H. pylori infection.
authors.affiliation: State Key Laboratories for Agrobiotechnology, China Agriculture University, Beijing 100094, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Xu
authors.forename: Yao-Feng
authors.initials: YF
authors.lastname: Zhao
authors.forename: Zheng-Xing
authors.initials: ZX
authors.lastname: Lian
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Fan
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Yu
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Dai
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Niu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Lennart
authors.initials: L
authors.lastname: Hammarström
authors.forename: Thomas
authors.initials: T
authors.lastname: Borén
authors.forename: Rolf
authors.initials: R
authors.lastname: Sjöström
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001789:Blood Group Antigens / Q000378:metabolism*; D005260:Female; D030841:Food, Genetically Modified; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006041:Goats; D016480:Helicobacter pylori / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000201:enzymology; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000201:enzymology / Q000502:physiology*
chemical_list: D001789:Blood Group Antigens; D007983:Lewis Blood Group Antigens; D005647:Fucosyltransferases