Radioligand-binding methods for membrane preparations and intact cells.
The radioligand-binding assay is a relatively simple but powerful tool for studying G-protein-coupled receptors. There are three basic types of radioligand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined: and (3) kinetic experiments from which the forward and reverse rate constants for radioligand binding can be determined. Detailed methods for typical radioligand-binding assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.
Bylund
David B
DB
Department of Pharmacology, University of Nebraska Medical Center, Omaha, USA.
Deupree
Jean D
JD
Toews
Myron L
ML
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011869:Radioligand Assay / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*; D013997:Time Factors
D043562:Receptors, G-Protein-Coupled