Peyer's patch dendritic cells process viral antigen from apoptotic epithelial cells in the intestine of reovirus-infected mice.
We explored the role of Peyer's patch (PP) dendritic cell (DC) populations in the induction of immune responses to reovirus strain type 1 Lang (T1L). Immunofluorescence staining revealed the presence of T1L structural (sigma1) and nonstructural (sigmaNS) proteins in PPs of T1L-infected mice. Cells in the follicle-associated epithelium contained both sigma1 and sigmaNS, indicating productive viral replication. In contrast, sigma1, but not sigmaNS, was detected in the subepithelial dome (SED) in association with CD11c(+)/CD8alpha(-)/CD11b(lo) DCs, suggesting antigen uptake by these DCs in the absence of infection. Consistent with this possibility, PP DCs purified from infected mice contained sigma1, but not sigmaNS, and PP DCs from uninfected mice could not be productively infected in vitro. Furthermore, sigma1 protein in the SED was associated with fragmented DNA by terminal deoxy-UTP nick-end labeling staining, activated caspase-3, and the epithelial cell protein cytokeratin, suggesting that DCs capture T1L antigen from infected apoptotic epithelial cells. Finally, PP DCs from infected mice activated T1L-primed CD4(+) T cells in vitro. These studies show that CD8alpha(-)/CD11b(lo) DCs in the PP SED process T1L antigen from infected apoptotic epithelial cells for presentation to CD4(+) T cells, and therefore demonstrate the cross-presentation of virally infected cells by DCs in vivo during a natural viral infection.
Fleeton
Marina N
MN
Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 10, Room 11N228, Bethesda, MD 20892, USA.
Contractor
Nikhat
N
Leon
Francisco
F
Wetzel
J Denise
JD
Dermody
Terence S
TS
Kelsall
Brian L
BL
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D017209:Apoptosis; D039481:CD11b Antigen / Q000096:biosynthesis; D039521:CD11c Antigen / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology*; D005260:Female; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000821:virology*; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D030102:Orthoreovirus, Mammalian / Q000235:genetics / Q000378:metabolism*; D010581:Peyer's Patches / Q000378:metabolism / Q000821:virology*
D000956:Antigens, Viral; D039481:CD11b Antigen; D039521:CD11c Antigen; D016827:CD8 Antigens; C431698:CD8alpha antigen; D007633:Keratins