Bacteriorhodopsin conjugates as anchors for supported membranes.
The sophistication of supported lipid bilayer membranes has increased steadily as new applications are being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate. Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin-PEG3400-bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymer conjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-based anchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres. This method could provide a new route for the stability enhancement of supported lipid bilayer membrane assemblies.
Sharma
Manoj K
MK
Departments of Chemical and Biomedical Engineering, The City College of the City University of New York, 140th Street and Convent Avenue, New York 10031, USA.
Jattani
Harsha
H
Gilchrist
M Lane
ML
D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D018613:Microscopy, Confocal; D008863:Microspheres; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019809:Streptavidin / Q000737:chemistry
D008051:Lipid Bilayers; D008081:Liposomes; D001436:Bacteriorhodopsins; D019809:Streptavidin; D008239:Lysine