title: Expression of a modified Cry1Ie gene in E. coli and in transgenic tobacco confers resistance to corn borer.
abstract: The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.
authors.affiliation: State Key Laboratory for Agrobiotechnology, National Center for Maize Improvement, China Agricultural University, Beijing.
authors.forename: Yun-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Fu-Ping
authors.initials: FP
authors.lastname: Song
authors.forename: Kang-Lai
authors.initials: KL
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005798:Genes, Bacterial; D007313:Insecta; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D010947:Plants, Toxic / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*
chemical_list: D025801:Ubiquitin