The genes encoding bovine SP-A, SP-D, MBL-A, conglutinin, CL-43 and CL-46 form a distinct collectin locus on Bos taurus chromosome 28 (BTA28) at position q.1.8-1.9.
Collectins are a group of C-type lectins involved in the innate immune system, where they mediate and modulate clearance of pathogens. The health status of cattle is of major economical and ethical concern; therefore, the study of bovine collectins is of importance. The collectins conglutinin, CL-43 and CL-46 are only present in Bovidae and the characterization of their genes indicates that they are structural descendants of another collectin, lung surfactant protein D (SP-D). In this study, we assembled BAC clones into a contig spanning 330-1150 kb, which includes the bovine genes encoding the collectins SP-A (SFTPA), SP-D (SFTPD), mannan-binding lectin A (MBL1), CL-43 (COLEC9), CL-46 (COLEC13) and conglutinin (COLEC8). In the same contig, we also identified a gene that potentially encodes a novel conglutinin-like collectin (COLEC14). The arrangement of STFPA, SFTPD and MBL1 is homologous to the organization found in humans and mice, whereas the Bovidae-specific collectin genes, COLEC8, COLEC9 and COLEC13, extend from SFTPD. Proximal to the collectin locus at BTA28q1.8-1.9, and included in the contig, we found the microsatellite IDVGA8, which may be a valuable marker for tracking polymorphisms in the linked collectin genes.
Gjerstorff
M
M
Department of Immunology and Microbiology, University of Southern Denmark, Odense, Denmark.
Hansen
S
S
Jensen
B
B
Dueholm
B
B
Horn
P
P
Bendixen
C
C
Holmskov
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D016000:Cluster Analysis; D037222:Collectins / Q000235:genetics*; D015723:Gene Library; D023061:Gene Order; D037601:Mannose-Binding Lectin / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D012712:Serum Globulins / Q000235:genetics
D037222:Collectins; D037601:Mannose-Binding Lectin; D037662:Pulmonary Surfactant-Associated Protein A; D037663:Pulmonary Surfactant-Associated Protein D; D012712:Serum Globulins; C024014:conglutinin; C086954:collectin-43 protein, bovine