Characterization of small unilamellar vesicles using solvatochromic pi* indicators and particle sizing.
A suite of small unilamellar vesicles (SUVs) composed of mixtures of phospholipids and cholesterol (CH) or the synthetic surfactant dihexadecyl phosphate (DHP) and cholesterol was investigated using a homologous series of solvatochromic pi* indicators coupled with size exclusion methods and photon correlation spectroscopy (PCS). The solvatochromic method, which is based on the measurement of solvent-dependent shifts in lambda(max) from UV-Vis spectra of solubilized indicators, was used to quantify the dipolarity and polarizability (pi*) of probe solvation environments. The partitioning of the series of individual di-n-alkyl-p-nitroaniline (DNAP) pi* indicators in PG(24)PC(46)Chol(30) and DHP(70)Chol(30) SUVs was examined as a function of the head group structure as well as the method of dye-vesicle preparation. Solubilization of the larger more hydrophobic probes in the bilayer portion of PG(24)PC(46)Chol(30) SUVs was aided through physical entrapment. Such physical methods were not needed for the smaller indicators or for the range of indicators in the DHP(70)Chol(30) dispersions. Extrusion and size-exclusion chromatographic methodologies for the preparation of physically entrapped dopants in SUVs of fixed size range demonstrated that the larger (longer alkyl chain) dopants in the series resided in PG(24)PC(46)Chol(30) liposomes with a wider range of sizes, while the smaller more polar solutes tended to be entrapped in smaller vesicles with a narrower size range.
Carrozzino
Jennifer M
JM
Department of Chemistry, North Carolina State University, Box 8204, Raleigh, NC 27695-8204, USA.
Fuguet
Elisabet
E
Helburn
Robin
R
Khaledi
Morteza G
MG
D001703:Biophysics / Q000379:methods*; D002784:Cholesterol / Q000737:chemistry; D002845:Chromatography; D004396:Coloring Agents / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D007202:Indicators and Reagents / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry; D008823:Micelles; D008956:Models, Chemical; D010316:Particle Size; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D017785:Photons; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D004396:Coloring Agents; D004136:Dinitrobenzenes; D007202:Indicators and Reagents; D008081:Liposomes; D008823:Micelles; D010715:Phosphatidylglycerols; D010743:Phospholipids; D002784:Cholesterol; C031536:dinitroaminophenol