title: BHK cells behaviour on laser treated polydimethylsiloxane surface.
abstract: The surface of polydimethylsiloxane (PDMS) was modified using a CO2-pulsed laser to evaluate the changes in physical and biological properties of the treated surface. Attachment of anchorage dependent cells, namely baby hamster kidney (BHK) fibroblastic cells, on PDMS surface was investigated in stationary culture conditions. BHK cell adhesion and growth on the PDMS surfaces were studied using scanning electron microscopy (SEM) and optical microscopy. To evaluate the surface wettability, water drop contact angles were determined. The laser treated PDMS surfaces showed high hydrophobicity and low cell adhesion, no spreading and growth in comparison with the unmodified PDMS. It was found that both the wettability and surface structure of the PDMS surface control cell attachment and growth.
authors.affiliation: Biomaterial Department, Iran Polymer and Petrochemical Institute, P.O. Box 14965/115, Tehran, Iran.
authors.forename: M T
authors.initials: MT
authors.lastname: Khorasani
authors.forename: H
authors.initials: H
authors.lastname: Mirzadeh
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry; D002455:Cell Division / Q000502:physiology; D006224:Cricetinae; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000528:radiation effects; D005347:Fibroblasts / Q000502:physiology*; D007668:Kidney / Q000166:cytology; D007834:Lasers; D016062:Porosity; D012828:Silicones / Q000737:chemistry* / Q000528:radiation effects; D013499:Surface Properties; D017155:Wettability
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; D002245:Carbon Dioxide; C013830:baysilon