title: Purification and structural determination of hematopoietic stem cell-stimulating monoacetyldiglycerides from Cervus nippon (deer antler).
abstract: A mixture of monoacetyldiglycerides was newly isolated from the chloroform extract of antlers of Cervus nippon, guided by the hematopoietic stimulation of stem cells. The structures of monoacetyldiglycerides were determined by various spectroscopic methods: FAB MS, CID tandem MS, and 1D and 2D NMR. A mixture of at least nine inseparable sn-3-monoacetyldiglycerides was identified: 1 [C(39)H(72)O(6) (C16 : 0/C18 : 1)], 2 [C(39)H(72)O(6) (C18 : 1/C16 : 0)], 3 [C(39)H(70)O(6) (C16 : 0/C18 : 2)], 4 [C(39)H(70)O(6) (C18 : 2/C16 : 0)], 5 [C(41)H(74)O(6) (C18 : 0/C18 : 2), 6 [C(41)H(74)O(6) (C18 : 2/C18 : 0)], 7 [C(41)H(74)O(6) (C18 : 1/C18 : 1)], 8 [C(43)H(74)O(6) (C18 : 0/C20 : 4)], and 9 [C(43)H(74)O(6) (C20 : 4/C18 : 0)]. Among these nine monoacetyldiglycerides in deer antlers, compound 3 was one of the major compounds and was efficiently synthesized from glycerol. Spectral data of synthetic monoacetyldiglyceride 3 were compared with the corresponding data for the mixture of natural monoacetyldiglycerides. The mixture of natural monoacetyldiglycerides from deer antlers showed potent activity on the hematopoiesis (stimulation index=1.40+/-0.05, p<0.02 at 1 microg/ml), and synthetic monoacetyldiglyceride 3 showed even better activity (stimulation index=1.54+/-0.12, p<0.001, at 1 microg/ml).
authors.affiliation: Department of Chemistry and Division of Molecular Life Sciences, Ewha Womans University, Seoul, Korea.
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Yang
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: So-Hye
authors.initials: SH
authors.lastname: Cho
authors.forename: Mi-Geum
authors.initials: MG
authors.lastname: Kim
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Seo
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Gil-Ja
authors.initials: GJ
authors.lastname: Jhon
authors.forename: So-Yeop
authors.initials: SY
authors.lastname: Han
mesh_terms: D000818:Animals; D000999:Antlers; D003670:Deer; D004075:Diglycerides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice
chemical_list: D004075:Diglycerides