Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase.
BACKGROUND
Human telomerase reverse transcriptase (hTERT) is catalytic subunit of human telomerase.


METHODS
We studied the immortalization of a series of human dental and periodontal cells by ectopic expression of hTERT and co-expression of hTERT with human papilloma virus 16 (HPV16) or simian virus 40 (SV40). Differentiation abilities of the established cell lines were studied in terms of the mineralized matrix formation and gene expression.


RESULTS
We established immortalized gingival fibroblasts by hTERT, dental papilla and periodontal ligament cells by hTERT and HPV16, and pulp cells by hTERT and SV40. The papilla and pulp cells showed mineralization and dentin sialophosphoprotein (DSPP) expression when cultured in the presence of beta-glycerophosphate. The immortalized periodontal ligament cells did not show mineralization or DSPP expression, although expressions of alkaline phosphatase, osteopontin and osteocalcin were detected.


CONCLUSIONS
These cell lines will be useful tools for studying the repair and regeneration of dental and periodontal tissues and various diseases including odontogenic tumors.
Kamata
Nobuyuki
N
First Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Tokushima, Kuramoto-cho, Tokushima, Japan. noka@dent.tokushima-u.ac.jp
Fujimoto
Ryoichi
R
Tomonari
Mayumi
M
Taki
Masayuki
M
Nagayama
Masaru
M
Yasumoto
Shigeru
S
D000818:Animals; D002461:Cell Line, Transformed / Q000166:cytology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins; D003771:Dental Papilla / Q000166:cytology*; D003782:Dental Pulp / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000502:physiology; D015870:Gene Expression; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin; D010513:Periodontal Ligament / Q000166:cytology*; D010750:Phosphoproteins; D011498:Protein Precursors / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis; D019098:Telomerase / Q000378:metabolism*; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
D024042:Collagen Type I; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D053495:Osteopontin; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D019098:Telomerase; D000099311:Dentin Sialophosphoprotein; C506895:SPP1 protein, human; C506896:Spp1 protein, mouse