title: Quantitation of the sulfur mustard metabolites 1,1'-sulfonylbis[2-(methylthio)ethane] and thiodiglycol in urine using isotope-dilution Gas chromatography-tandem mass spectrometry.
abstract: Sulfur mustard (HD), or bis(2-chloroethyl)sulfide, has several urinary metabolites that can be measured to assess human exposure. These metabolites include the simple hydrolysis product thiodiglycol (TDG) and its oxidative analogue, TDG-sulfoxide, as well as metabolites of the glutathione/b-lyase pathway 1,1'-sulfonylbis[2-(methyl-sulfinyl)ethane] (SBMSE) and 1-methyl-sulfinyl-2-[(methylthio)ethyl-sulfonyl]ethane (MSMTESE). Current methods focus on either the TDG or the b-lyase metabolites. We have developed a single method that measures products of both metabolic branches, with the reduced compound of SBMSE and MSMTESE, 1,1'-sulfonylbis [2(methylthio)ethane] (SBMTE), as the definitive analyte and TDG as a confirmation analyte. Sample preparation included b-glucuronidase hydrolysis for TDG-glucuronide conjugates, titanium trichloride reduction of sulfoxides to SBMTE and TDG, solid-phase extraction, and a chemical derivatization. We analyzed samples using gas chromatography-tandem mass spectrometry with quantitation using isotope-dilution calibration. The method limits of detection for TDG and SBMTE were 0.5 ng/mL and 0.25 ng/mL, respectively, with relative standard deviations of less than 10%. Urine samples from individuals with no known exposure to mustard agent HD had measurable concentrations of TDG, but no SBMTE was detected. The geometric mean concentration of TDG was 3.43 ng/mL, with concentrations ranging from < 0.5 ng/mL to 20 ng/mL.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Boyer
authors.forename: Doris
authors.initials: D
authors.lastname: Ash
authors.forename: Dana B
authors.initials: DB
authors.lastname: Barr
authors.forename: Carrie L
authors.initials: CL
authors.lastname: Young
authors.forename: W J
authors.initials: WJ
authors.lastname: Driskell
authors.forename: Ralph D
authors.initials: RD
authors.lastname: Whitehead
authors.forename: Maria
authors.initials: M
authors.lastname: Ospina
authors.forename: Kerry E
authors.initials: KE
authors.lastname: Preston
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Woolfitt
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Martinez
authors.forename: L A Pete
authors.initials: LA
authors.lastname: Silks
authors.forename: John R
authors.initials: JR
authors.lastname: Barr
mesh_terms: D000818:Animals; D004781:Environmental Exposure / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D051381:Rats; D012016:Reference Values; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000652:urine*; D013450:Sulfones / Q000737:chemistry / Q000652:urine*; D013454:Sulfoxides / Q000737:chemistry / Q000652:urine*
chemical_list: D013438:Sulfhydryl Compounds; D013450:Sulfones; D013454:Sulfoxides; C073452:1,1'-sulfonylbis(2-(methylsulfinyl)ethane); C047519:2,2'-thiodiethanol