Fully automated synthesis system of 3'-deoxy-3'-[18F]fluorothymidine.
We developed a new fully automated method for the synthesis of 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT), by modifying a commercial FDG synthesizer and its disposable fluid pathway. Optimal labeling condition was that 40 mg of precursor in acetonitrile (2 mL) was heated at 150 degrees C for 100 sec, followed by heating at 85 degrees C for 450 sec and hydrolysis with 1 N HCl at 105 degrees C for 300 sec. Using 3.7 GBq of [18F]F- as starting activity, [18F]FLT was obtained with a yield of 50.5 +/- 5.2% (n = 28, decay corrected) within 60.0 +/- 5.4 min including HPLC purification. With 37.0 GBq, we obtained 48.7 +/- 5.6% (n = 10). The [18F]FLT showed the good stability for 6 h. This new automated synthesis procedure combines high and reproducible yields with the benefits of a disposable cassette system.
Oh
Seung Jun
SJ
Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, South Korea. sjoh@amc.seoul.kr
Mosdzianowski
Christoph
C
Chi
Dae Yoon
DY
Kim
Jung Young
JY
Kang
Se Hun
SH
Ryu
Jin Sook
JS
Yeo
Jeong Seok
JS
Moon
Dae Hyuk
DH
D001331:Automation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D015224:Dideoxynucleosides / Q000138:chemical synthesis*; D004355:Drug Stability; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet
D015224:Dideoxynucleosides; D019275:Radiopharmaceuticals; C002854:alovudine