Ectopic expression of DICE1 suppresses tumor cell growth.
The tumor suppressor gene DICE1 is located within a previously reported critical region of loss of heterozygosity on chromosome 13q14.3. Expression of the remaining DICE1 allele is down-regulated in non-small cell lung carcinomas. Ectopic expression of DICE1 cDNA by DICE1-green fluorescent protein fusion constructs resulted in inhibition of colony formation of human non-small cell lung carcinoma cell line SK-MES-1 and NCI-H520 and prostate carcinoma cell line DU145. In IGF-IR transformed Balb/c 3T3, DICE1 substantially sup-pressed growth in soft agar. These results demonstrate that DICE1 has a growth-suppressing activity and interferes with anchorage-independent growth of IGF-IR transformed tumor cells dependent upon IGF-I signaling.
Wieland
Ilse
I
Institute of Human Genetics, Otto-von-Guericke-Universität, Magdeburg, Germany. ilse.wieland@medizin.uni-magdeburg.de
Sell
Christian
C
Weidle
Ulrich H
UH
Wieacker
P
P
D000362:Agar / Q000737:chemistry; D020816:Amino Acid Motifs; D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D016147:Genes, Tumor Suppressor; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D020365:RNA Helicases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012269:Ribosomal Proteins; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*
D018076:DNA, Complementary; C121427:INTS6 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; D012313:RNA; D007334:Insulin-Like Growth Factor I; D000362:Agar; D020365:RNA Helicases