title: Liquid chromatographic and mass spectrometric analysis of human serum acid alpha-1-glycoprotein.
abstract: Human serum acid alpha-1-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls.
authors.affiliation: National Institute of Oncology, Department of Biochemistry, PO Box 21, H 1122 Budapest, Hungary. kremmer@oncol.hu
authors.forename: T
authors.initials: T
authors.lastname: Kremmer
authors.forename: E
authors.initials: E
authors.lastname: Szöllösi
authors.forename: M
authors.initials: M
authors.lastname: Boldizsár
authors.forename: B
authors.initials: B
authors.lastname: Vincze
authors.forename: K
authors.initials: K
authors.lastname: Ludányi
authors.forename: T
authors.initials: T
authors.lastname: Imre
authors.forename: G
authors.initials: G
authors.lastname: Schlosser
authors.forename: K
authors.initials: K
authors.lastname: Vékey
mesh_terms: D001769:Blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009961:Orosomucoid / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009961:Orosomucoid