Antioxidant activity of leaf extracts from Bauhinia monandra.
Bauhinia monandra Kurz. is used in Brazil for the treatment of diabetes. Since this activity may be correlated with the presence of antioxidant compounds, leaf extracts of B. monandra were evaluated for their radical scavenging capacity (RSC). An ethanolic extract was taken up in aqueous methanol and partitioned with hexane, chloroform, ethyl acetate to yield three organic extracts together with remaining aqueous extract. The RSC was determined spectrophotometrically using 1,1-diphenylpicrylhydrazyl free radical (DPPH). The chloroform and ethyl acetate extracts were the most appropriate as sources of antioxidant compounds as shown by their inhibition concentration (IC50) and inhibition percentage (IP) values. The antioxidant activity of such extracts was attributed to the presence of three compounds of different polarities (flavonoids and steroids). The chloroform and ethyl acetate extracts exhibited an IC50 of approximately 2 mg/g DPPH and IP values in the range of 60-65%. The results indicate that the extracts of B. monandra have a very potent antioxidant activity, compared with the pure catechins used as positive controls and with other plant extracts.
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Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, S/N Cidade Universitária, Pernambuco, Brazil.
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D000085:Acetates; D000975:Antioxidants / Q000378:metabolism*; D036481:Bauhinia / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000378:metabolism*; D002725:Chloroform; D002855:Chromatography, Thin Layer; D016166:Free Radical Scavengers / Q000378:metabolism*; D006586:Hexanes; D006834:Hydrazines / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D010851:Picrates; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D013053:Spectrophotometry
D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006586:Hexanes; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; C007650:ethyl acetate; D002725:Chloroform; C004931:1,1-diphenyl-2-picrylhydrazyl