Decreased hypothalamic aromatization in female rats of true precocious puberty.
The true precocious puberty animal model induced by the single dose of danazol was used for investigating the expressions of hypothalamic aromatase in the advanced onset of puberty in rats. The day of vaginal opening and first estrus showed significant advancement in the model rats compared with the normal and vehicle rats (P < 0.01, respectively). The hypothalamic gonadotropin-releasing hormone (GnRH) mRNA expression increased significantly in the model rats compared with that in the normal and vehicle ones (P < 0.01). The levels of aromatase mRNA and protein expressions detected by RT-PCR and Western blot both decreased in the model rats compared with those in the normal and vehicle groups (P < 0.05). The results suggested that the hypothalamic aromatization might diminish in the onset of true precocious puberty of female rats.
Tian
Zhan-Zhuang
ZZ
Department of Neurobiology and Integrative Medicine, Shanghai Medical Center, Fudan University, P.O. Box 291, 138 Yi Xue Yuan Road, Shanghai 200032, PR China.
Zhao
Hong
H
Chen
Bo-Ying
BY
D000818:Animals; D001141:Aromatase / Q000096:biosynthesis* / Q000235:genetics; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D007031:Hypothalamus / Q000201:enzymology*; D011629:Puberty, Precocious / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012741:Sexual Maturation / Q000502:physiology*
D012333:RNA, Messenger; D001141:Aromatase