Direct identification of Mycobacterium haemophilum in skin lesions of immunocompromised patients by PCR-restriction endonuclease analysis.
PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 weeks required for culture isolation and identification by conventional methods.
Wang
S X
SX
Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608. gptwsx@sgh.com.sg
Sng
L H
LH
Leong
H N
HN
Tan
B H
BH
D004262:DNA Restriction Enzymes / Q000494:pharmacology; D006801:Humans; D016867:Immunocompromised Host; D018393:Mycobacterium haemophilum / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000293:injuries / Q000382:microbiology*
D004262:DNA Restriction Enzymes