The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.
We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
Ryder
Edward
E
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.
Blows
Fiona
F
Ashburner
Michael
M
Bautista-Llacer
Rosa
R
Coulson
Darin
D
Drummond
Jenny
J
Webster
Jane
J
Gubb
David
D
Gunton
Nicola
N
Johnson
Glynnis
G
O'Kane
Cahir J
CJ
Huen
David
D
Sharma
Punita
P
Asztalos
Zoltan
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Baisch
Heiko
H
Schulze
Janet
J
Kube
Maria
M
Kittlaus
Kathrin
K
Reuter
Gunter
G
Maroy
Peter
P
Szidonya
Janos
J
Rasmuson-Lestander
Asa
A
Ekström
Karin
K
Dickson
Barry
B
Hugentobler
Christoph
C
Stocker
Hugo
H
Hafen
Ernst
E
Lepesant
Jean Antoine
JA
Pflugfelder
Gert
G
Heisenberg
Martin
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Mechler
Bernard
B
Serras
Florenci
F
Corominas
Montserrat
M
Schneuwly
Stephan
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Preat
Thomas
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Roote
John
J
Russell
Steven
S
D000818:Animals; D002869:Chromosome Aberrations; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional / Q000379:methods
D004251:DNA Transposable Elements