CD10 immunohistochemical staining enhances the histological detection of endometriosis.
OBJECTIVE
To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions.


METHODS
Retrospective cohort study.


METHODS
Tertiary care government research hospital.


METHODS
Thirty-one women with chronic pelvic pain.


METHODS
Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised.


METHODS
Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens.


RESULTS
In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45% vs. 35%). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis.


CONCLUSIONS
The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.
Potlog-Nahari
Clariss
C
Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892-1583, USA. naharic@mail.nih.gov
Feldman
Andrew L
AL
Stratton
Pamela
P
Koziol
Deloris E
DE
Segars
James
J
Merino
Maria J
MJ
Nieman
Lynnette K
LK
D000328:Adult; D015331:Cohort Studies; D004396:Coloring Agents; D003937:Diagnosis, Differential; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005260:Female; D005456:Fluorescent Dyes; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D004396:Coloring Agents; D005456:Fluorescent Dyes; D015260:Neprilysin; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin