title: Viral and immunological factors associated with breast milk transmission of SIV in rhesus macaques.
abstract: BACKGROUND
  The viral and host factors involved in transmission of HIV through breastfeeding are largely unknown, and intervention strategies are urgently needed to protect at-risk populations. To evaluate the viral and immunological factors directly related to milk transmission of virus, we have evaluated the disease course of Simian Immunodeficiency Virus (SIV) in lactating rhesus macaques (Macaca mulatta) as a model of natural breast milk transmission of HIV.
  
  
  RESULTS
  Fourteen lactating macaques were infected intravenously with SIV/DeltaB670, a pathogenic isolate of SIV and were pair-housed with their suckling infants throughout the disease course. Transmission was observed in 10 mother-infant pairs over a one-year period. Two mothers transmitted virus during the period of initial viremia 14-21 days post inoculation (p.i.) and were classified as early transmitters. Peak viral loads in milk and plasma of early transmitters were similar to other animals, however the early transmitters subsequently displayed a rapid progressor phenotype and failed to control virus expression as well as other animals at 56 days p.i. Eight mothers were classified as late transmitters, with infant infection detected at time points in the chronic stage of the maternal SIV disease course (81 to 360 days). Plasma viral loads, CD4+ T cell counts and SIV-specific antibody titers were similar in late transmitters and non-transmitters. Late breast milk transmission, however, was correlated with higher average milk viral loads and more persistent viral expression in milk 12 to 46 weeks p.i. as compared to non-transmitters. Four mothers failed to transmit virus, despite disease progression and continuous lactation.
  
  
  CONCLUSIONS
  These studies validate the SIV-infected rhesus macaque as a model for breast milk transmission of HIV. As observed in studies of HIV-infected women, transmission occurred at time points throughout the period of lactation. Transmission during the chronic stage of SIV-infection correlated with a threshold level of virus expression as well as more persistent shedding in milk. This model will be a valuable resource for deciphering viral and host factors responsible for transmission of HIV through breastfeeding.
authors.affiliation: Department of Microbiology, Immunology, Parasitology, Louisiana State University Health Sciences Center, New Orleans, USA. aamede@lsuhsc.edu
authors.forename: Angela M
authors.initials: AM
authors.lastname: Amedee
authors.forename: Jenna
authors.initials: J
authors.lastname: Rychert
authors.forename: Nedra
authors.initials: N
authors.lastname: Lacour
authors.forename: Lynn
authors.initials: L
authors.lastname: Fresh
authors.forename: Marion
authors.initials: M
authors.lastname: Ratterree
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D007774:Lactation; D008253:Macaca mulatta / Q000276:immunology / Q000821:virology*; D008895:Milk, Human / Q000276:immunology* / Q000821:virology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000635:transmission*; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000302:isolation & purification*