title: Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acid.
abstract: The severe acute respiratory syndrome virus (SARS) is a coronavirus that instigated regional epidemics in Canada and several Asian countries in 2003. The newly identified SARS coronavirus (SARS-CoV) can be transmitted among humans and cause severe or even fatal illnesses. As preventive vaccine development takes years to complete and adverse reactions have been reported to some veterinary coronaviral vaccines, anti-viral compounds must be relentlessly pursued. In this study, we analyzed the effect of aurintricarboxylic acid (ATA) on SARS-CoV replication in cell culture, and found that ATA could drastically inhibit SARS-CoV replication, with viral production being 1000-fold less than that in the untreated control. Importantly, when compared with IFNs alpha and beta, viral production was inhibited by more than 1000-fold as compared with the untreated control. In addition, when compared with IFNs alpha and beta, ATA was approximately 10 times more potent than IFN alpha and 100 times more than interferon beta at their highest concentrations reported in the literature previously. Our data indicated that ATA should be considered as a candidate anti-SARS compound for future clinical evaluation.
authors.affiliation: National Microbiology Laboratory, Health Canada, 1015 Arlington St., Winnipeg, MB, Canada R3E 3R2. runtao_he@hc-sc.gc.ca
authors.forename: Runtao
authors.initials: R
authors.lastname: He
authors.forename: Anton
authors.initials: A
authors.lastname: Adonov
authors.forename: Maya
authors.initials: M
authors.lastname: Traykova-Adonova
authors.forename: Jingxin
authors.initials: J
authors.lastname: Cao
authors.forename: Todd
authors.initials: T
authors.lastname: Cutts
authors.forename: Elsie
authors.initials: E
authors.lastname: Grudesky
authors.forename: Yvon
authors.initials: Y
authors.lastname: Deschambaul
authors.forename: Jody
authors.initials: J
authors.lastname: Berry
authors.forename: Michael
authors.initials: M
authors.lastname: Drebot
authors.forename: Xuguang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001312:Aurintricarboxylic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D015203:Reproducibility of Results; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000188:drug therapy / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D001312:Aurintricarboxylic Acid