A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng.
Leaf explants collected from flowering plants of Vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA; 13.2-88.8 microM) and indole-3-acetic acid (IAA; 0.0 -85.6 microM). Combination of BA (66.6 microM) and IAA (28.5 microM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf explants. For optimized multiplication, BA:IAA (2:1 microM) was essential at 22.2- 88.8 microM of BA. Re-cultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 microM of BA and 5.7-28.5 microM of IAA. Rooting of shoots (> 95%) occurred in medium containing banana pulp (75 gl(-1)) and IAA (5.7 microM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.
Decruse
S William
SW
Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695 562, India. willdic@rediffmail.com
Gangaprasad
A
A
Seeni
S
S
Menon
V Sarojini
VS
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D018519:Meristem / Q000187:drug effects / Q000254:growth & development*; D029595:Orchidaceae / Q000187:drug effects / Q000254:growth & development*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D011687:Purines
D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids; D011687:Purines; C030737:indoleacetic acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin