Should the 'bleach microscopy method' be recommended for improved case detection of tuberculosis? Literature review and key person analysis.
METHODS
It has been proposed that the sensitivity of direct sputum smear microscopy can be improved if sputum is liquefied with sodium hypochlorite (NaOCl or household bleach), and concentrated by centrifugation before acid-fast staining.


OBJECTIVE
To summarise the results of the studies of the bleach method for improved sensitivity of sputum microscopy and to describe the opinions and knowledge of key persons in National Tuberculosis Control Programmes (NTPs) about this method.


METHODS
We searched Medline, EMBASE and Web of Science for studies comparing the bleach method to direct sputum smear microscopy in low- or middle-income countries. Each study was assessed regarding methodology and field applicability. We also sent out questionnaires concerning the bleach method to key persons in NTPs in 85 countries.


RESULTS
In 15 of the 19 studies identified there was a statistically significant improvement in the proportion of positive tests or sensitivity ranging from 7-253%. The majority (73%) of the key persons had heard of the bleach method. Forty-four per cent thought it could improve case detection in their countries, while 49% did not know; 93% of them would promote the bleach method; the most common reasons for doing so would be recommendations from the WHO or the IUATLD, or favourable studies performed in their own country. The bleach method was used routinely in only three countries.


CONCLUSIONS
There is enough evidence to recommend the evaluation and introduction of the bleach method in most settings where mycobacterial culture is not performed routinely.
Angeby
K A K
KA
Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden. kristian.angeby@smi.ki.se
Hoffner
S E
SE
Diwan
V K
VK
D002498:Centrifugation; D004202:Disinfectants; D006801:Humans; D008853:Microscopy / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D012973:Sodium Hypochlorite; D013048:Specimen Handling; D013183:Sputum / Q000166:cytology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D014944:World Health Organization
D004202:Disinfectants; D012973:Sodium Hypochlorite