La autoantigen is necessary for optimal function of the poliovirus and hepatitis C virus internal ribosome entry site in vivo and in vitro.
Translation of poliovirus and hepatitis C virus (HCV) RNAs is initiated by recruitment of 40S ribosomes to an internal ribosome entry site (IRES) in the mRNA 5' untranslated region. Translation initiation of these RNAs is stimulated by noncanonical initiation factors called IRES trans-activating factors (ITAFs). The La autoantigen is such an ITAF, but functional evidence for the role of La in poliovirus and HCV translation in vivo is lacking. Here, by two methods using small interfering RNA and a dominant-negative mutant of La, we demonstrate that depletion of La causes a dramatic reduction in poliovirus IRES function in vivo. We also show that 40S ribosomal subunit binding to HCV and poliovirus IRESs in vitro is inhibited by a dominant-negative form of La. These results provide strong evidence for a function of the La autoantigen in IRES-dependent translation and define the step of translation which is stimulated by La.
Costa-Mattioli
Mauro
M
Department of Biochemistry and McGill Cancer Center, McGill University, McIntyre Medical Building, Montreal, Quebec, Canada H3G 1Y6.
Svitkin
Yuri
Y
Sonenberg
Nahum
N
D020121:5' Untranslated Regions; D001324:Autoantigens / Q000737:chemistry*; D005799:Genes, Dominant; D017930:Genes, Reporter; D006367:HeLa Cells; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008957:Models, Genetic; D009154:Mutation; D017955:Poliovirus / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry / Q000502:physiology*; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D000097585:SS-B Antigen
D020121:5' Untranslated Regions; D001324:Autoantigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012261:Ribonucleoproteins; D000097585:SS-B Antigen