title: Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection.
abstract: This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD, a column packed with titanium dioxide resin was used. The titanium dioxide column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve.
authors.affiliation: Department of Biological Sciences, Faculty of Science, Nara Women's University, Kitauoyanishimachi, Nara City, Nara 630-8506, Japan. sekiguchi@dionex.co.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sekiguchi
authors.forename: Naoto
authors.initials: N
authors.lastname: Mitsuhashi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Inoue
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yagisawa
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Mimura
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004563:Electrochemistry / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013403:Sugar Phosphates / Q000032:analysis*; D014025:Titanium / Q000737:chemistry*
chemical_list: D013403:Sugar Phosphates; C009495:titanium dioxide; D014025:Titanium