An improvement of the site-directed mutagenesis method by combination of megaprimer, one-side PCR and DpnI treatment.
Wei
Dongsheng
D
Department of Microbiology, Nankai University, Tianjin 300071, PR China.
Li
Mingchun
M
Zhang
Xinxin
X
Xing
Laijun
L
D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific