Functional and chromosomal clustering of genes responsive to 5-bromodeoxyuridine in human cells.
5-Bromodeoxyuridine immediately and dramatically induces senescence-associated genes in human cells. We examined changes in gene expression in HeLa cells using cDNA microarrays containing ca. 39,000 human genes or ESTs. Addition of 5-bromodeoxyuridine for 4 days changed expression of 2.6% of them twice more (1.5%) or less (1.1%) than control levels. We functionally categorized 191 genes that showed greater than four times increase or decrease, and found that they have a various function. These genes were assigned to various human chromosomes, and half of them seemed to cluster at a few regions on individual chromosomes. These results suggest that multiple genes collectively act to induce cellular senescence and chromatin structure has a role in expression of the genes.
Minagawa
Sachi
S
Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, 244-0813, Japan.
Nakabayashi
Kazuhiko
K
Fujii
Michihiko
M
Scherer
Stephen W
SW
Ayusawa
Dai
D
D015152:Blotting, Northern; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000235:genetics; D002874:Chromosome Mapping; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D005810:Multigene Family / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
D001973:Bromodeoxyuridine