[Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis].
OBJECTIVE
To observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.


METHODS
Semen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.


RESULTS
Sperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters, including average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), straightness (STR) and linearity (LIN), except heat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP, VSL and VCL) and moving pattern parameters (ALH) significantly decreased, while that of LIN and STR significantly increased in post-thaw samples.


CONCLUSIONS
CASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples.
Liu
Meiyi
M
Human Semen Bank, Guangdong Family Planning Hospital, Guangzhou, Guangdong 510600, China. dxlmyi@pub.guangzhou.gd.cn
Deng
Shunmei
S
Ma
Chunjie
C
Chen
Aiping
A
Jiang
Yanjia
Y
Wen
Renqian
R
Wang
Qiling
Q
Tang
Lixin
L
Huang
Jianchu
J
Yao
Xiaotao
X
D015925:Cryopreservation; D003936:Diagnosis, Computer-Assisted; D005615:Freezing; D006801:Humans; D008297:Male; D013081:Sperm Motility