title: Determination of levoglucosan in atmospheric fine particulate matter.
abstract: A microanalytical method suitable for the quantitative determination of the sugar anhydride levoglucosan in low-volume samples of atmospheric fine particulate matter (PM) has been developed and validated. The method incorporates two sugar anhydrides as quality control standards. The recovery standard sedoheptulosan (2,7-anhydro-beta-D-altro-heptulopyranose) in 20 microL solvent is added onto samples of the atmospheric fine PM and aged for 1 hr before ultrasonic extraction with ethylacetate/ triethylamine. The extract is reduced in volume, an internal standard is added (1,5-anhydro-D-mannitol), and a portion of the extract is derivatized with 10% by volume N-trimethylsilylimidazole. The derivatized extract is analyzed by gas chromatography/mass spectrometry (GC/MS). The recovery of levoglucosan using this procedure was 69 +/- 6% from five filters amended with 2 microg levoglucosan, and the reproducibility of the assay is 9%. The limit of detection is approximately 0.1 microg/mL, which is equivalent to approximately 3.5 ng/m3 for a 10 L/min sampler or approximately 8.7 ng/m3 for a 4 L/min personal sampler (assuming 24-hr integrated samples). We demonstrated that levoglucosan concentrations in collocated samples (expressed as ng/m3) were identical irrespective of whether samples were collected by PM with aerodynamic diameter < or = 2.5 microm or PM with aerodynamic diameter < or = 10 microm impactors. It was also demonstrated that X-ray fluorescence analysis of samples of atmospheric PM, before levoglucosan determinations, did not alter the levels of levoglucosan.
authors.affiliation: Department of Environmental and Occupational Health Sciences, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195, USA. simpson1@u.washington.edu
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Simpson
authors.forename: Russell L
authors.initials: RL
authors.lastname: Dills
authors.forename: Bethany S
authors.initials: BS
authors.lastname: Katz
authors.forename: David A
authors.initials: DA
authors.lastname: Kalman
mesh_terms: D000336:Aerosols; D000393:Air Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000031:analogs & derivatives* / Q000032:analysis*; D013052:Spectrometry, X-Ray Emission
chemical_list: D000336:Aerosols; D000393:Air Pollutants; C014989:1,6-anhydro-beta-glucopyranose; D005947:Glucose