Cellular levels of trp RNA-binding attenuation protein in Bacillus subtilis.
Expression of the Bacillus subtilis trp genes is negatively regulated by an 11-subunit trp RNA-binding attenuation protein (TRAP), which is activated to bind RNA by binding l-tryptophan. We used Western blotting to estimate that there are 200 to 400 TRAP 11-mer molecules per cell in cells grown in either minimal or rich medium.
McCabe
Barbara C
BC
Department of Biological Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
Gollnick
Paul
P
D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D014364:Tryptophan