Comparison of two sites in Mobile Bay using in vivo biomarkers in largemouth bass, sediment bioassays, and sediment contaminant analysis.
This research addresses whether chemical contaminants are having a biologically measurable effect on largemouth bass (Micropterus salmoides), an important fish species in the Mobile Bay National Estuary. Bass and sediment samples were collected in September 2001 from Big Bateau and D'Olive Bays; the latter has been suggested to be an environmentally impacted site. There was a significant difference in the age distribution of bass collected from the two sites. However, none of the animals showed any evidence of reproductive activity or had mature gametes. The age and gonadosomatic index were greater in animals collected from the Bateau site. Fish liver microsome ethoxyresorufin-O-deethylase (EROD) activities (elevated in the presence of CYP1A-inducing contaminants) were not significantly different between sites but were significantly higher in older bass. Plasma steroid concentrations in younger animals (less than 1 year old) tended to be higher and more variable than the concentrations found in older animals. Once the significant effects of age on plasma steroid concentrations were removed, an analysis of the residual change in steroids revealed no differences in testosterone or estradiol between animals collected at the two sites. Sediments were collected for chemical extraction and analysis in two bioassays: the H4IIE rat hepatoma EROD assay and the yeast estrogen screen (YES assay). The H4IIE bioassay indicated the presence of sediment BaP induction equivalents between 72 and 320 ng/g, whereas the YES assay indicated that potent estrogenic substances were not present in the sediment samples. The H4IIE bioassay results were higher for D'Olive samples, which was consistent with higher PAH concentrations in those sediments. Taken together the biomarkers and chemical analysis suggested generally low organic contamination at these two sites.
Annavarapu
S
S
Department of Pharmacology and Environmental Toxicology Research Program, University of Mississippi, 38677, USA.
Foran
C M
CM
Gardinali
P
P
Metzger
C
C
Willett
K L
KL
D000407:Alabama; D000818:Animals; D001492:Bass / Q000254:growth & development* / Q000378:metabolism; D001667:Binding, Competitive; D001681:Biological Assay; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring; D004958:Estradiol / Q000097:blood; D019015:Geologic Sediments / Q000737:chemistry*; D006801:Humans; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D011960:Receptors, Estrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013739:Testosterone / Q000097:blood; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; D019363:Cytochrome P-450 CYP1A1