title: The fate of dendritic cells in a mouse model of liver ischemia/reperfusion injury.
abstract: Ischemia/reperfusion during liver transplantation triggers a complex cascade of inflammatory events that may lead to organ dysfunction. Herein, we investigated the consequences of hepatic ischemia/reperfusion on liver dendritic cells. Liver damage was documented by increased levels of serum alanine aminotransferase and by histopathology showing large areas of hepatocyte cytolysis. MHC class II+ CD45-B220 F4/80 dendritic cells were detected in necrotic areas 20 hours after reperfusion. Dendritic cells freshly isolated from reperfused livers displayed a mature phenotype characterized by upregulated expression of B7 costimulatory molecules; MHC-class II, and CD1d molecules. As shown by real-time PCR, IL-10, and TGF-beta mRNA accumulated in liver dendritic cells isolated after reperfusion, whereas IL-12p40 mRNA levels were decreased and IFN-gamma mRNA levels were unchanged. These results suggest that hepatic ischemia/reperfusion results in maturation of dendritic cells, which preferentially produce inhibitory cytokines.
authors.affiliation: Institute for Medical Immunology-Laboratory of Experimental Immunology, Universite Libre de Bruxelles, Brussels.
authors.forename: P
authors.initials: P
authors.lastname: Loi
authors.forename: F
authors.initials: F
authors.lastname: Paulart
authors.forename: B
authors.initials: B
authors.lastname: Pajak
authors.forename: N
authors.initials: N
authors.lastname: Nagy
authors.forename: I
authors.initials: I
authors.lastname: Salmon
authors.forename: M
authors.initials: M
authors.lastname: Moser
authors.forename: M
authors.initials: M
authors.lastname: Goldman
authors.forename: V
authors.initials: V
authors.lastname: Flamand
mesh_terms: D000410:Alanine Transaminase / Q000032:analysis; D000818:Animals; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008099:Liver / Q000098:blood supply; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009336:Necrosis; D015427:Reperfusion Injury / Q000276:immunology* / Q000473:pathology
chemical_list: D000949:Histocompatibility Antigens Class II; D000410:Alanine Transaminase