title: The ATC1 gene encodes a cell wall-linked acid trehalase required for growth on trehalose in Candida albicans.
abstract: After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino acid sequence shows that Atc1p contains an N-terminal hydrophobic signal peptide and 20 potential sites for N-glycosylation. C. albicans homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two ATC chromosomal alleles. Analysis of these null mutants shows that Atc1p is localized in the cell wall and is required for growth on trehalose as a carbon source. An Atc1p endowed with acid trehalase activity was obtained by an in vtro transcription-translation coupled system. These results strongly suggest that ATC1 is the structural gene encoding cell wall acid trehalase in C. albicans. Determinations of ATC1 mRNA expression as well as acid trehalase activity in the presence and absence of glucose point out that ATC1 gene is regulated by glucose repression.
authors.affiliation: Area de Microbiología, Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Pedreño
authors.forename: Sergi
authors.initials: S
authors.lastname: Maicas
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Argüelles
authors.forename: Rafael
authors.initials: R
authors.lastname: Sentandreu
authors.forename: Eulogio
authors.initials: E
authors.lastname: Valentin
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D015153:Blotting, Western; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D002244:Carbon / Q000737:chemistry; D002455:Cell Division; D002473:Cell Wall / Q000201:enzymology*; D002474:Cell-Free System; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006720:Homozygote; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014158:Transcription, Genetic; D014198:Trehalase / Q000502:physiology; D014199:Trehalose / Q000737:chemistry
chemical_list: C118431:ATC1 protein, S cerevisiae; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon; D004247:DNA; D014199:Trehalose; D014198:Trehalase; D005947:Glucose