[Experimental lung carcinogenic in vivo study of aflatoxin G1 in NIH mice].
OBJECTIVE
Aflatoxin G1 (AFG1) is a member of the carcinogenic aflatoxin family produced by aspergillus flavus. It is a major contaminating mycotoxin in food in areas of China with high cancer incidence. The purpose of this study is to explore the carcinogenic effects of AFG1 in NIH mice.


METHODS
NIH mice were randomly divided into three groups. Two experimental groups were treated intragastrically by gavage with AFG1 3 microg/kg and AFG1 30 microg/kg respectively, 3 times a week for 24 weeks. The control group was treated with normal saline. All mice were fed with food that was free of AFGs as confirmed by HPLC analysis. The mice were weighed every week throughout the entire experiment, and then sacrificed and examined pathologically at the 58th and 74th weeks respectively.


RESULTS
Compared with control mice receiving no AFG1, bronchial epithelial hyperplasia, alveolar hyperplasia and adenocarcinoma of lung were observed in mice receiving AFG1 treatment. The incidences of bronchial epithelial hyperplasia, alveolar hyperplasia and adenocarcinoma of lung were 60.0%, 10.0% and 30.0% for mice receiving 3 microg/kg AFG1 and 28.6%, 35.7%, 42.9% for mice receiving 30 microg/kg of the toxin, respectively.


CONCLUSIONS
Oral administration of AFG1 can induce hyperplastic lesions and adenocarcinoma of lung in NIH mice.
Huang
Xiang-Hua
XH
Lab of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China.
Zhang
Xiang-Hong
XH
Li
Yue-Hong
YH
Wang
Jun-Ling
JL
Yan
Xia
X
Xing
Ling-Xiao
LX
Wang
Feng-Rong
FR
D000230:Adenocarcinoma / Q000139:chemically induced* / Q000473:pathology*; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D001231:Aspergillus flavus; D002273:Carcinogens / Q000633:toxicity; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology*; D051379:Mice; D011897:Random Allocation
D000348:Aflatoxins; D002273:Carcinogens; C027955:aflatoxin G1