title: Dominant lethal effect of 60Co gamma radiation in Biomphalaria glabrata (SAY, 1818).
abstract: The dominant lethal effects of gamma radiation of 60Co in the snail Biomphalaria glabrata were studied. Three groups of 13 wild-type snails were irradiated with single doses of 2.5; 10 and 20 Gy. Crossings were carried out at intervals of 7, 17, 23, 30 and 36 days after irradiation. The dominant lethal effect was observed only at the first crossing occurring 7 days after irradiation with 2.5 Gy. With 10 and 20 Gy, the induction of lethal mutations was detected at 7, 17 and 23 days after irradiation; a dose-response effect was observed. The effect was stronger 7 days after irradiation, decreasing in the succeeding crossings up to 30 days. Cell-killing effects on germ cells were detected in the crossings at 23 days and 30 days after irradiation with 20 Gy. After 36 days, frequencies of malformations resumed background levels; crossing rates partially recovered. These results show that gamma radiation affected all the stages of spermatogenesis. Germ cells at later phases were more sensitive to the mutagenic effect of radiation and the cell killing effects were observed on the youngest cells. This response was similar to the highly homogeneous pattern observed in widely different species and allowed us to estimate some parameters of spermatogenesis in B. glabrata.
authors.affiliation: Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, São Paulo, Brazil. letallarico2@butantan.gov.br
authors.forename: Lenita
authors.initials: L
authors.lastname: de Freitas Tallarico
authors.forename: Kayo
authors.initials: K
authors.lastname: Okazaki
authors.forename: Toshie
authors.initials: T
authors.lastname: Kawano
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Bragança Pereira
authors.forename: Eliana
authors.initials: E
authors.lastname: Nakano
mesh_terms: D000367:Age Factors; D000818:Animals; D003037:Cobalt Radioisotopes / Q000633:toxicity*; D003433:Crosses, Genetic; D004307:Dose-Response Relationship, Radiation; D004625:Embryo, Nonmammalian / Q000528:radiation effects; D005720:Gamma Rays; D005804:Genes, Lethal / Q000235:genetics; D008297:Male; D009154:Mutation / Q000235:genetics / Q000528:radiation effects*; D012908:Snails / Q000235:genetics / Q000528:radiation effects*; D013091:Spermatogenesis / Q000528:radiation effects; D013997:Time Factors
chemical_list: D003037:Cobalt Radioisotopes