From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels.
We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min.
Tegenfeldt
Jonas O
JO
Department of Physics, Lund University, SE-221 00 Lund, Sweden.
Prinz
Christelle
C
Cao
Han
H
Chou
Steven
S
Reisner
Walter W
WW
Riehn
Robert
R
Wang
Yan Mei
YM
Cox
Edward C
EC
Sturm
James C
JC
Silberzan
Pascal
P
Austin
Robert H
RH
D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D016678:Genome; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012680:Sensitivity and Specificity
D004247:DNA