title: Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling.
abstract: Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common malignancy of young men. Using genome-wide gene expression profiling, we identified >200 genes highly expressed in testicular CIS, including many never reported in testicular neoplasms. Expression was further verified by semiquantitative reverse transcription-PCR and in situ hybridization. Among the highest expressed genes were NANOG and POU5F1, and reverse transcription-PCR revealed possible changes in their stoichiometry on progression into embryonic carcinoma. We compared the CIS expression profile with patterns reported in embryonic stem cells (ESCs), which revealed a substantial overlap that may be as high as 50%. We also demonstrated an over-representation of expressed genes in regions of 17q and 12, reported as unstable in cultured ESCs. The close similarity between CIS and ESCs explains the pluripotency of CIS. Moreover, the findings are consistent with an early prenatal origin of TGCTs and thus suggest that etiologic factors operating in utero are of primary importance for the incidence trends of TGCTs. Finally, some of the highly expressed genes identified in this study are promising candidates for new diagnostic markers for CIS and/or TGCTs.
authors.affiliation: University Department of Growth and Reproduction, Rigshospitalet, Section GR-5064, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. Kristian.Almstrup@biobase.dk
authors.forename: Kristian
authors.initials: K
authors.lastname: Almstrup
authors.forename: Christina E
authors.initials: CE
authors.lastname: Hoei-Hansen
authors.forename: Ute
authors.initials: U
authors.lastname: Wirkner
authors.forename: Jonathon
authors.initials: J
authors.lastname: Blake
authors.forename: Christian
authors.initials: C
authors.lastname: Schwager
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Ansorge
authors.forename: John E
authors.initials: JE
authors.lastname: Nielsen
authors.forename: Niels E
authors.initials: NE
authors.lastname: Skakkebaek
authors.forename: Ewa
authors.initials: E
authors.lastname: Rajpert-De Meyts
authors.forename: Henrik
authors.initials: H
authors.lastname: Leffers
mesh_terms: D002278:Carcinoma in Situ / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002877:Chromosomes, Human / Q000235:genetics; D005784:Gene Amplification; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000473:pathology*; D013736:Testicular Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*