Expression profiling by high-throughput immunohistochemistry.
Immunohistochemistry (IHC) provides valuable information on expression of proteins within tissues at a cellular and subcellular level. Recent developments in the practice of IHC now make it possible to contemplate using this technique as a high-throughput expression profiling system. Advances have been made in creation and use of tissue microarrays, in automated IHC and in image capture/analysis. Each of these technologies are reviewed and issues surrounding their use considered. The success of high-throughput IHC is also dependent on both generation and screening of appropriate antibodies. Antibody-related issues which are likely to affect the success of high-throughput IHC, such as specificity, sensitivity, fixation choice, etc., are also considered.
Warford
Anthony
A
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
Howat
William
W
McCafferty
John
J
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D000918:Antibody Specificity; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods*; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D051379:Mice
D000906:Antibodies