One liver, three recipients: segment IV from split-liver procedures as a source of hepatocytes for cell transplantation.
Hepatocyte transplantation is emerging as a possible treatment for patients with acute liver failure and liver-based metabolic disorders. With the limited availability of donor tissue, it is important to find new sources of liver tissue for isolation of high-quality hepatocytes. Segment IV with or without the caudate lobe was removed during three split-liver procedures. Hepatocytes were isolated from the tissues using a collagenase perfusion technique under strict sterile conditions. The mean number of hepatocytes that were isolated was 5.14 x 10(8) cells with a mean cell viability of 89%. Two of the hepatocyte preparations were used for cell transplantation in a 1-day-old boy with an antenatal diagnosis of a severe urea cycle defect caused by ornithine transcarbamylase deficiency. The six recipients of split-liver grafts demonstrated no complications related to the removal of segment IV. Segment IV with or without the caudate lobe obtained from split-liver procedures is potentially a good source of high-quality hepatocytes for cell transplantation.
Mitry
Ragai R
RR
Institute of Liver Studies, Guy's, King's and St. Thomas' School of Medicine, and King's College Hospital, Denmark Hill, London, United Kingdom.
Dhawan
Anil
A
Hughes
Robin D
RD
Bansal
Sanjay
S
Lehec
Sharon
S
Terry
Claire
C
Heaton
Nigel D
ND
Karani
John B
JB
Mieli-Vergani
Giorgina
G
Rela
Mohamed
M
D000293:Adolescent; D000328:Adult; D022781:Hepatocytes / Q000637:transplantation*; D006801:Humans; D007231:Infant, Newborn; D008107:Liver Diseases / Q000601:surgery; D008297:Male; D008661:Metabolism, Inborn Errors / Q000601:surgery; D009955:Ornithine Decarboxylase / Q000172:deficiency; D009927:Tissue and Organ Procurement / Q000379:methods*; D016896:Treatment Outcome
D009955:Ornithine Decarboxylase