Phylogenetic analysis of symbiotic and free-living cyanobacterial cultures using DNA amplification fingerprinting.
DNA amplification fingerprinting (DAF) of 17 cyanobacterial isolates belonging to symbiotic and free-living forms of 6 different genera was done. The dendrogram analysis of 17 cyanobacterial cultures revealed three major clusters. All Westiellopsis cultures formed the first major cluster and their nucleotide relatedness ranged between 71-93%. In the second major cluster, the symbiotic cyanobacterium Anabaena sp dominated and all Anabaena cultures showed 60-75% similarity with each other. Nostoc muscorum is related to Anabaena variabilis by 85% and formed the third major cluster. The dendrogram analysis of cyanobacterial isolates clearly revealed that free-living cyanobacterial cultures were closely related with each other and were diverse from the symbiotic forms.
Prabina
B Jeberlin
BJ
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, India.
Kumar
K
K
Kannaiyan
S
S
D000458:Cyanobacteria / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D013559:Symbiosis
D017931:DNA Primers; D004269:DNA, Bacterial