Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma.
BACKGROUND
It has been found that TH1-related cytokines can decrease the accumulation of eosinophils in lung tissue and relieve airway constriction.


OBJECTIVE
Dendritic cells (DCs) have been found to prime naive T-helper cells efficiently. In this study, DCs infected with TH1 cytokine-expressing adenovirus can be used to induce antigen-specific TH1 cells for treatment in an animal model of asthma.


METHODS
Cytokine gene-modulated DCs pulsed with ovalbumin antigen (OVA) were injected intravenously into naive mice 1 week before sensitization with OVA antigen. The mice were then monitored for OVA-specific IgE, airway inflammatory cell infiltration, and airway hyperresponsiveness in the study.


RESULTS
Significant levels of IL-12 or IL-18 were expressed by Ad-IL-12 or Ad-IL-18 infected, bone marrow-derived DCs. Ad-IL-12 and Ad-IL-18 co-infected DCs effectively, decreasing sera anti-OVA IgE antibody levels, lung eosinophilia, and airway hyperresponsiveness.


CONCLUSIONS
We concluded that DCs modulated by TH1-prone cytokine-expressing adenoviruses can alleviate TH2-type airway inflammation in a murine model and can provide possible therapeutic application for DCs in asthma.
Ye
Yi-Ling
YL
Department of Medical Technology, Chung Hwa College of Medical Technology, Tainan, Taiwan, Republic of China.
Lee
Yueh-Lun
YL
Chuang
Zen-Jai
ZJ
Lai
Huai-Jean
HJ
Chen
Chun-Chi
CC
Tao
Mi-Hua
MH
Chiang
Bor-Luen
BL
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000150:complications / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000150:complications / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004802:Eosinophilia / Q000150:complications / Q000276:immunology*; D018664:Interleukin-12 / Q000276:immunology; D020382:Interleukin-18 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D016207:Cytokines; D020382:Interleukin-18; D018664:Interleukin-12