title: Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp. MZ1T.
abstract: Conditions were developed for the reproducible production, isolation and characterization of a novel microbial extracellular polysaccharide believed to be involved in transient viscous bulking at an industrial wastewater treatment plant. The exopolysaccharide was extracted from cell-free culture supernatants of Thauera sp. strain MZ1T grown on a minimal medium with succinate. The purified polymer was found to be approximately 260 kDa in size by gel-permeation chromatography. The GC-MS analysis of the alditol acetate and per-O-trimethylsilyl methyl glycoside derivatives revealed that the exopolysaccharide was composed of four monosaccharides including: rhamnose, galacturonic acid, N-acetylglucosamine and N-acetylfucosamine. Glucose, which also appeared at low levels, is most likely from a co-eluting glucan. The FTIR and NMR spectroscopic analyses further revealed the presence of esterified component groups on the polymer. These results represent the first published description of a polysaccharide from a member of the genus Thauera, and lay the foundation for a deeper understanding of the factors potentially involved in zoogloeal cluster formation and viscous bulking.
authors.affiliation: Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Allen
authors.forename: Karen T
authors.initials: KT
authors.lastname: Welch
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Prebyl
authors.forename: David C
authors.initials: DC
authors.lastname: Baker
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Meyers
authors.forename: Gary S
authors.initials: GS
authors.lastname: Sayler
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis*; D002850:Chromatography, Gel; D003470:Culture Media / Q000737:chemistry; D005643:Fucose / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D007220:Industrial Waste; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D012210:Rhamnose / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D019802:Succinic Acid / Q000378:metabolism; D013402:Sugar Alcohols / Q000737:chemistry; D020628:Thauera / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
chemical_list: D002241:Carbohydrates; D003470:Culture Media; D006603:Hexuronic Acids; D007220:Industrial Waste; D011135:Polysaccharides, Bacterial; D013402:Sugar Alcohols; D005643:Fucose; C059377:N-acetylfucosamine; C007819:galacturonic acid; D019802:Succinic Acid; D005947:Glucose; D012210:Rhamnose; D000117:Acetylglucosamine