A set of BAC clones spanning the human genome.
Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.
Krzywinski
Martin
M
BC Cancer Agency Genome Sciences Center and BC Cancer Agency, Vancouver, BC V5Z 4E6, Canada.
Bosdet
Ian
I
Smailus
Duane
D
Chiu
Readman
R
Mathewson
Carrie
C
Wye
Natasja
N
Barber
Sarah
S
Brown-John
Mabel
M
Chan
Susanna
S
Chand
Steve
S
Cloutier
Alison
A
Girn
Noreen
N
Lee
Darlene
D
Masson
Amara
A
Mayo
Michael
M
Olson
Teika
T
Pandoh
Pawan
P
Prabhu
Anna-Liisa
AL
Schoenmakers
Eric
E
Tsai
Miranda
M
Albertson
Donna
D
Lam
Wan
W
Choy
Chik-On
CO
Osoegawa
Kazutoyo
K
Zhao
Shaying
S
de Jong
Pieter J
PJ
Schein
Jacqueline
J
Jones
Steven
S
Marra
Marco A
MA
D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D015894:Genome, Human; D006801:Humans; D020161:Physical Chromosome Mapping