title: Functional and chromosomal clustering of genes responsive to 5-bromodeoxyuridine in human cells.
abstract: 5-Bromodeoxyuridine immediately and dramatically induces senescence-associated genes in human cells. We examined changes in gene expression in HeLa cells using cDNA microarrays containing ca. 39,000 human genes or ESTs. Addition of 5-bromodeoxyuridine for 4 days changed expression of 2.6% of them twice more (1.5%) or less (1.1%) than control levels. We functionally categorized 191 genes that showed greater than four times increase or decrease, and found that they have a various function. These genes were assigned to various human chromosomes, and half of them seemed to cluster at a few regions on individual chromosomes. These results suggest that multiple genes collectively act to induce cellular senescence and chromatin structure has a role in expression of the genes.
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, 244-0813, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Minagawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakabayashi
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Scherer
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D015152:Blotting, Northern; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000235:genetics; D002874:Chromosome Mapping; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D005810:Multigene Family / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods
chemical_list: D001973:Bromodeoxyuridine