Stable and specific expression of 4-coumarate:coenzyme A ligase gene (4CL1) driven by the xylem-specific Pto4CL1 promoter in the transgenic tobacco.
The ability of 4-coumarate:coenzyme A ligase promoter from Populus tomentosa (Pto4CL1p) to drive expression of the GUS reporter gene and 4-coumarate:coenzyme A ligase gene in tobacco has been studied using transgenic plants produced by Agrobacterium-mediated transformation. Intense GUS histochemical staining was detected in the xylem of stem in transgenic tobacco plants carrying the 1140 bp Pto4CL1p promoter. To further investigate the regulation function of the tissue-specific expression promoter, Pto4CL1p, a binary vector containing Pto4CL1p promoter fused with 4CL1 gene was transferred into tobacco. The activity of the 4CL1 enzyme doubled in the stems of transgenic tobacco but did not increase in the leaves. The content of lignin was increased 25% in the stem but there was no increase in the leaves of transgenic tobacco.
Lu
Hai
H
College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P.R. China.
Zhao
Yan-Ling
YL
Jiang
Xiang-ning
XN
D001483:Base Sequence; D001709:Biotechnology / Q000379:methods; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000096:biosynthesis* / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D018547:Plant Stems; D030821:Plants, Genetically Modified; D032107:Populus / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics*; D014169:Transformation, Bacterial; D019076:Transgenes
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D008031:Lignin; D005966:Glucuronidase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase