Protective effect of fructose-1,6-bisphosphate in the cold storage solution for liver preservation in rat hepatic transplantation.
Fructose-1,6-bisphosphate (FBP) has been reported to have a protective effect on liver injury following ischemic/reperfusion periods. FBP maintains ATP levels and thereby cellular energy metabolism, which is important to the liver during cold preservation. In the present study, we evaluated the effects of FBP on the composition of storage solutions for cold liver preservation. Adult male Wistar rats were randomly divided into three experimental groups. Hepatic perfusion and preservation were performed with UW, UW plus 10 mmol/L FBP (UWM), and FBP 10 mmol/L (FBPS) alone solutions. Biochemical measurements of AST, ALT, and TBARS were performed on samples of the cold storage solution at 0, 12, 18, and 24 hours preservation. FBPS and UW solutions showed similar preservation grades during 18 hours. Addition of 10 mmol/L of FBP to UW solution induced liver injury and a poor preservation grade. FBP appears to protect the liver from injury caused by free radicals when the preservation time is less than 18 hours. Therefore, FBP may exert a protective effect for the preservation of livers during cold storage, and could represent an important component of new cold storage solutions.
Moresco
R N
RN
Laboratório de Pesquisa em Biofísica, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil. rnmoresco@yahoo.com.br
Santos
R C V
RC
Alves Filho
J C F
JC
Cunha
A A
AA
Dos Reis
C
C
Reichel
C L
CL
De Oliveira
J R
JR
D000241:Adenosine; D000410:Alanine Transaminase / Q000032:analysis; D000493:Allopurinol; D000818:Animals; D005635:Fructosediphosphates; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000502:physiology; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D005635:Fructosediphosphates; D007328:Insulin; D019188:Organ Preservation Solutions; D017392:Thiobarbituric Acid Reactive Substances; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; C027652:fructose 2,6-diphosphate; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose