title: Mass spectrometric identification of RNA binding proteins from dried EMSA gels.
abstract: Electrophoretic mobility shift assays (EMSA) are commonly employed for the analysis of nucleic acid/ protein interactions with a native gel system. Here, we report a method to identify RNA binding proteins from a dried EMSA gel by mass spectrometry following autoradiography. Compared to wet gel exposure, our approach resulted in an improved protein identification sensitivity and RNA/protein complex isolation accuracy. The method described here is useful for the large scale characterization of RNA- or DNA-protein complexes.
authors.affiliation: Institute of Plant Science, Swiss Federal Institute of Technology, Zürich, Switzerland.
authors.forename: Dominique
authors.initials: D
authors.lastname: Stenger
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Gruissem
authors.forename: Sacha
authors.initials: S
authors.lastname: Baginsky
mesh_terms: D001345:Autoradiography; D024202:Electrophoretic Mobility Shift Assay; D005782:Gels / Q000737:chemistry; D013058:Mass Spectrometry; D016601:RNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*
chemical_list: D005782:Gels; D016601:RNA-Binding Proteins