title: Performance of the MagNA pure LC robot for extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from urine and swab specimens.
abstract: DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.
authors.affiliation: School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA. cgaydos@jhmi.edu
authors.forename: Nicholas
authors.initials: N
authors.lastname: Dalesio
authors.forename: Vince
authors.initials: V
authors.lastname: Marsiglia
authors.forename: Andrew
authors.initials: A
authors.lastname: Quinn
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Gaydos
mesh_terms: D001437:Bacteriuria / Q000382:microbiology*; D002584:Cervix Uteri / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000652:urine; D005260:Female; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial