Japanese herbal medicine, Saiko-keishi-to, prevents gut ischemia/reperfusion-induced liver injury in rats via nitric oxide.
OBJECTIVE
To determine whether Saiko-keishi-to (TJ-10), a Japanese herbal medicine, could protect liver injury induced by gut ischemia/reperfusion (I/R), and to investigate the role of NO.


METHODS
Male Wistar rats were exposed to 30-min gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment. Plasma tumor necrosis factor (TNF) levels and alanine aminotransferase (ALT) activities were measured. TJ-10 1 g/(kg.d) was intragastrically administered to rats for 7 d. A NO synthase inhibitor was administered.


RESULTS
In control rats, gut I/R elicited increases in the number of stationary leukocytes, and plasma TNF levels and ALT activities were mitigated by pretreatment with TJ-10. Pretreatment with the NO synthase inhibitor diminished the protective effects of TJ-10 on leukostasis in the liver, and the increase of plasma TNF levels and ALT activities. Pretreatment with TJ-10 increased plasma nitrite/nitrate levels.


CONCLUSIONS
TJ-10 attenuates the gut I/R-induced hepatic microvascular dysfunction and sequential hepatocellular injury via enhancement of NO production.
Horie
Yoshinori
Y
Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan. yhorie@sc.itc.keio.ac.jp
Kajihara
Mikio
M
Mori
Shuka
S
Yamagishi
Yoshiyuki
Y
Kimura
Hiroyuki
H
Tamai
Hironao
H
Kato
Shinzo
S
Ishii
Hiromasa
H
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008102:Liver Circulation; D008107:Liver Diseases / Q000209:etiology* / Q000517:prevention & control*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*
D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide; C018142:saiko-keishi-to