title: Radioligand-binding methods for membrane preparations and intact cells.
abstract: The radioligand-binding assay is a relatively simple but powerful tool for studying G-protein-coupled receptors. There are three basic types of radioligand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined: and (3) kinetic experiments from which the forward and reverse rate constants for radioligand binding can be determined. Detailed methods for typical radioligand-binding assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.
authors.affiliation: Department of Pharmacology, University of Nebraska Medical Center, Omaha, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Bylund
authors.forename: Jean D
authors.initials: JD
authors.lastname: Deupree
authors.forename: Myron L
authors.initials: ML
authors.lastname: Toews
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011869:Radioligand Assay / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*; D013997:Time Factors
chemical_list: D043562:Receptors, G-Protein-Coupled