title: Detection of high-risk human papillomavirus (HPV) DNA by Hybrid Capture II in women referred due to atypical glandular cells in the primary screening.
abstract: We assessed the detection of high-risk human papillomavirus DNA (HPV-DNA) in women examined by a second Pap smear due to atypical glandular cells (AGC) detected in their screening Pap smear. In 91 women included in the study, a second Pap smear was taken and HPV-DNA test was performed using Hybrid Capture II (HC II). The second Pap smear showed no abnormalities in 28 (31%) cases, ACG in 17 (19%) cases, and high-grade squamous intraepithelial lesions (HSIL) or worse in 24 (26%). HC II test was positive in 36% of the cases. Considering the second Pap smear diagnosis, HPV-DNA was detected in 87% of the women with HSIL, 100% of women with in situ adenocarcinoma, and only in 11% of the women with no abnormalities. The use of the second Pap smear combined with HPV-DNA may improve the management of women with AGC in the primary screening.
authors.affiliation: Department of Obstetrics and Gynecology Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Eliane R Z M
authors.initials: ER
authors.lastname: Oliveira
authors.forename: Sophie F M
authors.initials: SF
authors.lastname: Derchain
authors.forename: Silvia H
authors.initials: SH
authors.lastname: Rabelo-Santos
authors.forename: Maria Cristina A
authors.initials: MC
authors.lastname: Westin
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Zeferino
authors.forename: Elisabete A
authors.initials: EA
authors.lastname: Campos
authors.forename: Kari J
authors.initials: KJ
authors.lastname: Syrjanen
mesh_terms: D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D008403:Mass Screening; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification*; D011230:Precancerous Conditions / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis*
chemical_list: D015344:DNA Probes, HPV