title: Surface attachment of nanoparticles using oligonucleotides.
abstract: Colloidal polymer particles are widely used in a variety of applications ranging from chromatography to surface modified bioreactors in protein arrays. In the present study, surface attachment of polystyrene particles to a polystyrene substrate has been performed using oligonucleotide hybridization. Thiolated complementary oligomers of cytosine and guanine have been covalently coupled to a pyridyl disulphide (PDS) modified polyethyleneglycol tether, forming part of a triblock copolymer which is adsorbed to the polystyrene surfaces via hydrophobic polypropylene oxide center blocks. The ability to withstand shear forces was studied using a laminar flow cell and the uptake of oligomers on the particles was quantified using two complementary techniques: UV-spectroscopy and sedimentation field flow fractionation. The possibility to tether particles in a flow cell suitable for practical use in e.g. a FIA-system is demonstrated.
authors.affiliation: Department of Surface Biotechnology, Biomedical Center, P.O. Box 577, Uppsala University, Uppsala 751 23, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Andersson
authors.forename: K
authors.initials: K
authors.lastname: Elihn
authors.forename: K
authors.initials: K
authors.lastname: Fromell
authors.forename: K D
authors.initials: KD
authors.lastname: Caldwell
mesh_terms: D001665:Binding Sites; D004220:Disulfides / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D004220:Disulfides; D009841:Oligonucleotides; D011108:Polymers; D011137:Polystyrenes; D011092:Polyethylene Glycols