G-protein-coupled receptor phosphorylation and palmitoylation.
It is now clear that nearly all G-protein-coupled receptors (GPCRs) are phosphorylated and palmitolyated. The process of receptor phosphorylation has been extensively studied because it offers a regulatory mechanism that is both rapid and dynamic. However, it has recently become clear that palmitoyaltion of GPCRs at C-terminal cysteine residues may also offer dynamic receptor modification. A growing number of GPCRs have been demonstrated to undergo rapid agonist-mediated changes in their palmitoylation status with functional implications to receptor signaling. This chapter aims to outline the methods we have used to investigate agonist-mediated changes in GPCR phosphorylation and palmitoylation.
Tobin
Andrew B
AB
Department of Cell Physiology and Pharmacology, University of Leicester, United Kingdom.
Wheatley
Mark
M
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D019308:Palmitic Acid / Q000378:metabolism; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D010766:Phosphorylation; D011233:Precipitin Tests / Q000379:methods; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D043587:Receptor, Muscarinic M3 / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
D010761:Phosphorus Radioisotopes; D043587:Receptor, Muscarinic M3; D043562:Receptors, G-Protein-Coupled; D014316:Tritium; D019308:Palmitic Acid