title: IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract.
abstract: Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-beta secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 +/- 3,519 vs 29,280 +/- 2,971 pg/ml). Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10), reflecting an increase in total fecal IgA antibodies (N = 10). The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production.
authors.affiliation: Laboratório de Alergia e Imunologia Clínica e Experimental (LIM-56), Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Maciel
authors.forename: A E
authors.initials: AE
authors.lastname: Fusaro
authors.forename: C R
authors.initials: CR
authors.lastname: Oliveira
authors.forename: E A
authors.initials: EA
authors.lastname: Futata
authors.forename: A J S
authors.initials: AJ
authors.lastname: Duarte
authors.forename: M N
authors.initials: MN
authors.lastname: Sato
mesh_terms: D000284:Administration, Oral; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D016207:Cytokines / Q000032:analysis; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D004391:Dust; D005260:Female; D007108:Immune Tolerance; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood*; D007422:Intestines / Q000276:immunology*; D008198:Lymph Nodes / Q000737:chemistry; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D039741:Antigens, Dermatophagoides; D016207:Cytokines; D004391:Dust; D007070:Immunoglobulin A; D007073:Immunoglobulin E