title: Transcession of DNA from bacteria to human cells in culture: a possible role in oncogenesis.
abstract: The human organism is continuously in close contact with microorganisms, especially bacteria. In the present work, by means of a real-time polymerase chain reaction (PCR) technique, we looked for the presence of a distinct bacterial gene in human cells. To this end, we cultured a human cell line, HL60, in a supernatant in which bacteria (Bacillus subtilis) had been grown. A transient transcession of bacterial DNA into the human cells was observed.
authors.affiliation: Extracellular Biology Research Laboratory, 7 route de Drize, 1227 Carouge/Geneva, Switzerland. panker@worldcom.ch
authors.forename: Philippe
authors.initials: P
authors.lastname: Anker
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zajac
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Lyautey
authors.forename: Christine
authors.initials: C
authors.lastname: Lederrey
authors.forename: Christophe
authors.initials: C
authors.lastname: Dunand
authors.forename: François
authors.initials: F
authors.lastname: Lefort
authors.forename: Hugh
authors.initials: H
authors.lastname: Mulcahy
authors.forename: Jack
authors.initials: J
authors.lastname: Heinemann
authors.forename: Maurice
authors.initials: M
authors.lastname: Stroun
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D002467:Cell Nucleus / Q000737:chemistry; D018920:Coculture Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D004270:DNA, Circular / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D018922:HL-60 Cells; D006801:Humans; D009369:Neoplasms / Q000209:etiology*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular