title: Application of oxidation-reduction assay for monitoring treatment of patients with pulmonary tuberculosis.
abstract: By oxidation-reduction assay, the viability of Mycobacterium tuberculosis in sputum specimens was evaluated. The technique is based on the Alamar Blue and Malachite Green dyes, which change their color in response to M. tuberculosis growth. The method is simple, permits visual reading of results, and is applicable for laboratories with limited resources.
authors.affiliation: Iranian National Reference TB Laboratory, National Research Institute of Tuberculosis and Lung Disease, Shaheed Beheshti University of Medical Sciences & Health Services, Darabad, Tehran 19556, Iran. pfarnia@hotmail.com
authors.forename: Parissa
authors.initials: P
authors.lastname: Farnia
authors.forename: Foroazan
authors.initials: F
authors.lastname: Mohammadi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirsaedi
authors.forename: Abolhasan Zia
authors.initials: AZ
authors.lastname: Zarife
authors.forename: Javad
authors.initials: J
authors.lastname: Tabatabee
authors.forename: Katayoon
authors.initials: K
authors.lastname: Bahadori
authors.forename: Muslem
authors.initials: M
authors.lastname: Bahadori
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Masjedi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Velayati
mesh_terms: D003116:Color; D004396:Coloring Agents / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development*; D010078:Oxazines; D010084:Oxidation-Reduction; D012394:Rosaniline Dyes / Q000378:metabolism; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000382:microbiology; D014966:Xanthenes
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D010078:Oxazines; D012394:Rosaniline Dyes; D014966:Xanthenes; C005095:malachite green; C005843:resazurin