Conditional beta1-integrin gene deletion in neural crest cells causes severe developmental alterations of the peripheral nervous system.
Integrins are transmembrane receptors that are known to interact with the extracellular matrix and to be required for migration, proliferation, differentiation and apoptosis. We have generated mice with a neural crest cell-specific deletion of the beta1-integrin gene to analyse the role of beta1-integrins in neural crest cell migration and differentiation. This targeted mutation caused death within a month of birth. The loss of beta1-integrins from the embryo delayed the migration of Schwann cells along axons and induced multiple defects in spinal nerve arborisation and morphology. There was an almost complete absence of Schwann cells and sensory axon segregation and defective maturation in neuromuscular synaptogenesis. Thus, beta1-integrins are important for the control of embryonic and postnatal peripheral nervous system development.
Pietri
Thomas
T
UMR144, CNRS, Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Eder
Olivier
O
Breau
Marie Anne
MA
Topilko
Piotr
P
Blanche
Martine
M
Brakebusch
Cord
C
Fässler
Reinhard
R
Thiery
Jean-Paul
JP
Dufour
Sylvie
S
D000818:Animals; D017353:Gene Deletion; D019012:Integrin beta1 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009154:Mutation; D009432:Neural Crest / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism; D017933:Peripheral Nervous System / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism; D012584:Sciatic Nerve / Q000002:abnormalities / Q000196:embryology / Q000378:metabolism
D019012:Integrin beta1