Performance of the MagNA pure LC robot for extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from urine and swab specimens.
DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.
Dalesio
Nicholas
N
School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA. cgaydos@jhmi.edu
Marsiglia
Vince
V
Quinn
Andrew
A
Quinn
Thomas C
TC
Gaydos
Charlotte A
CA
D001437:Bacteriuria / Q000382:microbiology*; D002584:Cervix Uteri / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification* / Q000652:urine; D005260:Female; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial