title: Serum and follicular fluid leptin levels in patients undergoing controlled ovarian hyperstimulation for in vitro fertilization cycle.
abstract: OBJECTIVE
  To determine serum and follicular fluid leptin levels in patients undergoing controlled ovarian hyperstimulation (COH) for an in vitro fertilization-embryo transfer (IVF-ET) cycle and their possible correlation to COH variables.
  
  
  METHODS
  Large university-based IVF unit.
  
  
  METHODS
  16 consecutive patients undergoing our routine IVF long gonadotrophin-releasing hormone-analog protocol. INTERVENTIONS AIND MAIN OUTCOME MEASURES: Blood was drawn three times during the COH cycle: 1) day on which adequate suppression was obtained (Day-S); 2) day of or prior to human chorionic gonadotrophin (hCG) administration (Day-hCG); and 3) day of ovum pick-up (Day-OPU). Levels of sex steroids and serum and follicular fluid leptin were compared among the three time points. Serum leptin was measured with a commercial two-site immunoradiometric assay.
  
  
  RESULTS
  Results showed significantly higher levels of serum leptin on Day-OPU and Day-hCG than on Day-S, and significantly higher follicular than serum leptin levels on Day-OPU. Though a significant correlation was observed between serum leptin and body mass index (BMI), no correlations were found between serum or follicular fluid leptin and serum sex-steroid levels or IVF treatment variables.
  
  
  CONCLUSIONS
  While serum leptin increases during COH for IVF, there is apparently no correlation of serum and follicular leptin levels with sex-steroid levels or IVF outcome.
authors.affiliation: Department of Obstetrics and Gynecology, Rabin Medical Center, Petah Tiqva, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Fisch
authors.forename: A
authors.initials: A
authors.lastname: Ben-Haroush
authors.forename: B
authors.initials: B
authors.lastname: Kaplan
authors.forename: M
authors.initials: M
authors.lastname: Hod
authors.forename: R
authors.initials: R
authors.lastname: Orvieto
mesh_terms: D000328:Adult; D006063:Chorionic Gonadotropin / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism; D006728:Hormones / Q000097:blood; D006801:Humans; D007247:Infertility, Female; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008137:Longitudinal Studies; D008597:Menstrual Cycle; D010062:Ovulation Induction; D011446:Prospective Studies
chemical_list: D006063:Chorionic Gonadotropin; D006728:Hormones; D020738:Leptin