S-nitrosation of thioredoxin in the nitrogen monoxide/superoxide system activates apoptosis signal-regulating kinase 1.
In the present study, we have investigated S-nitrosation of reactive thioredoxin (Trx) thiol groups in nitric oxide/superoxide system. We have found that Trx thiol groups are the targets for S-nitrosation by N2O3-like species generated in the system containing xanthine/xanthine oxidase (superoxide producing system) and DEA/NO-the *NO donating compound, however, they have shown low sensitivity to the *NO derived from DEA/NO. N2O3-dependent S-nitrosation of Trx at approximately 2-fold of NO excess compared to the superoxide amount resulted in dissociation and activation of apoptosis signal regulating kinase 1 (ASK1). However, approximately 4-fold of NO excess compared to a superoxide production preserved the level of dissociated ASK1 but decreased its activity due to the enzyme S-nitrosation.
Yasinska
Inna M
IM
Department of Biochemistry, Mechnikov Odessa National University, Per. Shampansky 2, Odessa 65058, Ukraine.
Kozhukhar
Anna V
AV
Sumbayev
Vadim V
VV
D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D048848:MAP Kinase Kinase Kinase 5; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D015538:Nitrosation; D010766:Phosphorylation; D011233:Precipitin Tests; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry*
D013481:Superoxides; D009569:Nitric Oxide; D013879:Thioredoxins; D013482:Superoxide Dismutase; D048848:MAP Kinase Kinase Kinase 5; D020930:MAP Kinase Kinase Kinases; C482858:MAP3K5 protein, human