Rattlesnake venom induces apoptosis by stimulating PC-PLC and upregulating the expression of integrin beta4, P53 in vascular endothelial cells.
In the previous studies, we found that phosphatidylcholine-specific phospholipase C (PC-PLC) was implicated in apoptosis induced by rattlesnake venom in vascular endothelial cells (VEC) [Biochem. Biophys. Res. Commun. (1997b) 223, 182]. In order to find out other signal elements in this pathway and the mechanisms by which PC-PLC mediates apoptosis induced by rattlesnake venom in VEC, the expression of integrin beta4 and P53 was evaluated when the activity of PC-PLC was suppressed by D609 (tricyclodecan-9-yl-xanthogenate), a specific inhibitor of this enzyme. The increase of integrin beta4 and P53 expression induced by the venom was markedly suppressed when apoptosis of VEC was inhibited by D609. The data indicated that integrin beta4 and P53 play important roles in signal transduction of apoptosis induced by rattlesnake venom, and that PC-PLC might regulate apoptosis by up-regulating the expression of integrin beta4 and P53 in VEC.
Zhao
Qitao
Q
Department of Biology, Institute of Developmental Biology, School of Life Science, Shandong University, No. 27 Southern Shanda Road, Jinan 250100, China.
Araki
Satohiko
S
Zhang
ShangLi
S
Miao
Junying
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D003435:Crotalid Venoms / Q000633:toxicity*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D039663:Integrin beta4 / Q000187:drug effects* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects* / Q000378:metabolism; D010738:Type C Phospholipases / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation
D003435:Crotalid Venoms; D039663:Integrin beta4; D016159:Tumor Suppressor Protein p53; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C