Characterization of lecithin:cholesterol acyltransferase expressed in a human lung cell line.
Lecithin:cholesterol acyltransferase (LCAT) is a key enzyme for the transfer of mammalian cholesterol from peripheral tissues to the liver. In patients deficient in LCAT, serum cholesterol levels rise and can lead to corneal opacity, proteinuria, anemia, and kidney failure. As early as 1968, relatively low volume transfusion of normal plasma was shown to temporarily correct the abnormal lipoprotein profiles in LCAT-deficient patients. However, despite the cloning, study, and extensive expression of LCAT in mammalian cell lines, there is still no viable, clinical therapy for LCAT deficiency. The current study was initiated to provide a source of recombinant human LCAT for enzyme replacement therapy. Accordingly, human LCAT has been cloned and expressed for the first time in a human cell line. The recombinant LCAT secreted by these cells was purified by phenyl-Sepharose chromatography, analyzed to determine the nature of its glycosylation, and tested for its enzymatic properties. The activity and basic kinetic parameters for the enzyme were determined using both a fluorescent water-soluble substrate and a macromolecular (proteoliposome) substrate. The enzymatic properties and the carbohydrate components of the recombinant LCAT were all sufficiently similar to those of the circulating human plasma enzyme, suggesting that this source of LCAT may be appropriate for use in some form of enzyme replacement therapy.
Lane
Steven B
SB
BetaGene, Inc., Dallas, TX, USA.
Tchedre
Kissaou T
KT
Nair
Maya P
MP
Thigpen
Anice E
AE
Lacko
Andras G
AG
D000740:Anemia / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D003318:Corneal Opacity / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D008168:Lung / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D011507:Proteinuria / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000627:therapeutic use; D051437:Renal Insufficiency / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D013379:Substrate Specificity
D011994:Recombinant Proteins; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase