Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation.
Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.
Chen
Yi-Kai
YK
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Leng
Chih-Hsiang
CH
Olivares
Heidi
H
Lee
Ming-Hui
MH
Chang
Yuan-Chih
YC
Kung
Wen-Mei
WM
Ti
Shih-Chieh
SC
Lo
Yu-Hui
YH
Wang
Andrew H-J
AH
Chang
Chia-Seng
CS
Bishop
Douglas K
DK
Hsueh
Yi-Ping
YP
Wang
Ting-Fang
TF
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D004843:Epistasis, Genetic; D008540:Meiosis / Q000502:physiology*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C513033:DMC1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C113830:HOP2 protein, S cerevisiae; C454441:MND1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA