title: A mass spectrometry plate reader: monitoring enzyme activity and inhibition with a Desorption/Ionization on Silicon (DIOS) platform.
abstract: A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.
authors.affiliation: Mass Consortium Corporation, 3030 Bunker Hill Street, San Diego, CA 92109, USA. zshen@massconsortium.com
authors.forename: Zhouxin
authors.initials: Z
authors.lastname: Shen
authors.forename: Eden P
authors.initials: EP
authors.lastname: Go
authors.forename: Alejandra
authors.initials: A
authors.lastname: Gamez
authors.forename: Junefredo V
authors.initials: JV
authors.lastname: Apon
authors.forename: Valery
authors.initials: V
authors.lastname: Fokin
authors.forename: Mike
authors.initials: M
authors.lastname: Greig
authors.forename: Manuel
authors.initials: M
authors.lastname: Ventura
authors.forename: John E
authors.initials: JE
authors.lastname: Crowell
authors.forename: Ola
authors.initials: O
authors.lastname: Blixt
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Stevens
authors.forename: M G
authors.initials: MG
authors.lastname: Finn
authors.forename: Gary
authors.initials: G
authors.lastname: Siuzdak
mesh_terms: D004791:Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D012825:Silicon / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004791:Enzyme Inhibitors; D004798:Enzymes; D012825:Silicon