Effects of partial liquid ventilation on lipopolysaccharide-induced inflammatory responses in rats.
To determine whether partial liquid ventilation (PLV) modified lung inflammatory response, we analyzed blood cytokine levels and cytokine mRNA expression in the lungs, using a rat model of endotoxemia. Thirty-six rats were allocated into one of four groups. The first group received conventional gas ventilation (CV group), the second group received 10 ml/kg perflubron intratracheally in combination with mechanical gas ventilation (PLV group), the third group received 20 mg/kg Escherichia coli lipopolyssacharide (LPS) intravenously in combination with mechanical gas ventilation (LPS group), and the fourth group received PLV and LPS (PLV + LPS group). Blood levels of TNF-alpha, IL-1beta, IL-6, IL-10, INF-gamma and IL-1 receptor antagonist were significantly increased in LPS and PLV + LPS groups. mRNA expression of pro- and anti-inflammatory cytokines in the lung tissue was also significantly increased in these groups. mRNA expression of IL-6 in PLV + LPS group was significantly increased in comparison with LPS group. Other cytokine mRNA expression including IL-10 and IL-1beta was also potentiated in PLV + LPS group, however this was not significant. Our results suggest that PLV does not protect the lungs against inflammation in systemic endotoxemia in rats.
Angelova
Milena
M
Department of Anesthesiology and Critical Care Medicine, Tokyo Medical and Dental University School of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Nakazawa
Koichi
K
Yokoyama
Kuninori
K
Makita
Koshi
K
D000818:Animals; D016207:Cytokines / Q000097:blood; D019446:Endotoxemia / Q000097:blood* / Q000209:etiology / Q000276:immunology; D004926:Escherichia coli; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000627:therapeutic use; D006842:Hydrocarbons, Brominated; D008070:Lipopolysaccharides; D021061:Liquid Ventilation / Q000379:methods; D008297:Male; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C003072:perflubron