An association analysis between ApoA1 polymorphisms and the high-density lipoprotein (HDL) cholesterol level and myocardial infarction (MI) in Japanese.
Association studies were performed to confirm the effect of polymorphisms in apolipoprotein A1 ( ApoA1) on the high-density lipoprotein cholesterol (HDL-C) level and the incidence of myocardial infarction (MI). A sequence analysis identified nine polymorphisms in ApoA1. After considering linkage disequilibrium, four polymorphisms in ApoA1 and four polymorphisms in the 5'-flanking regions and 3'-flanking regions from the JSNP database were determined in 1,880 subjects recruited from the Suita study, which represents the general population in Japan. Of the eight polymorphisms tested, the ApoA1 T84C polymorphism had the greatest effect on the levels of HDL-C ( P=0.0005, P(c)=0.0040 corrected by the Bonferroni method) and triglyceride ( P<0.0001, P(c)=0.0008). The ApoA1 MspI polymorphism was not associated with HDL-C or triglyceride levels. We confirmed that the ApoA1 T84C polymorphism was associated with the HDL-C level but not the triglyceride level in patients with MI ( n=637). Moreover, this polymorphism was associated with the incidence of MI in male subjects ( P=0.0326). A logistic analysis indicated that the frequency of MI in the CC genotype was lower than that in the CT+TT genotype ( P=0.0145, OR=0.4955, 95% CI: 0.2746-0.8525). The ApoA1 T84C polymorphism is an important marker for the HDL-C level and may be a new risk marker for MI in Japanese.
Shioji
Keisuke
K
Department of Epidemiology, Research Institute, National Cardiovascular Center, Suita, Japan. shiojikeisuke@kpe.biglobe.ne.jp.|Department of Cardiology, Kishiwada City Hospital, 1001 Gakuhara-cho, Kishiwada, Osaka, 596-8501, Japan. shiojikeisuke@kpe.biglobe.ne.jp.|The Organization for Pharmaceutical Safety and Research of Japan, Tokyo, Japan. shiojikeisuke@kpe.biglobe.ne.jp.
Mannami
Toshifumi
T
Department of Preventive Cardiology, National Cardiovascular Center, Suita, Japan.
Kokubo
Yoshihiro
Y
Department of Preventive Cardiology, National Cardiovascular Center, Suita, Japan.
Goto
Yoichi
Y
Division of Cardiology, National Cardiovascular Center, Suita, Japan.
Nonogi
Hiroshi
H
Division of Cardiology, National Cardiovascular Center, Suita, Japan.
Iwai
Naoharu
N
Department of Epidemiology, Research Institute, National Cardiovascular Center, Suita, Japan.
D000368:Aged; D016632:Apolipoprotein A-I / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D008076:Cholesterol, HDL / Q000097:blood*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000097:blood / Q000453:epidemiology* / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D008055:Lipids