Cell-surface-bound nucleic acids: Free and cell-surface-bound nucleic acids in blood of healthy donors and breast cancer patients.
Concentrations of extracellular DNA and RNA in the blood of healthy donors and patients with malignant and nonmalignant breast tumors were investigated. Cell-surface-bound extracellular DNA and RNA were detached by PBS-EDTA treatment or mild trypsin treatment of erythrocytes and leukocytes. In healthy donors, almost all extracellular nucleic acids (98%) are bound at the surface of blood cells. In the blood of cancer patients, extracellular nucleic acids were found in plasma and not at the cell surface. In patients with nonmalignant breast tumors, extracellular nucleic acids were found both at the surface of blood cells and in plasma. In healthy donors, the cell-surface-bound DNA is represented by 20-kbp DNA fragments and smaller fragments that varied in amounts in different fractions.
Laktionov
Pavel P
PP
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. lakt@niboch.nsc.ru
Tamkovich
Svetlana N
SN
Rykova
Elena Yu
EY
Bryzgunova
Olga E
OE
Starikov
Andrey V
AV
Kuznetsova
Nina P
NP
Vlassov
Valentin V
VV
D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D002614:Chelating Agents / Q000494:pharmacology; D004247:DNA / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D018226:Fibroadenoma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D009367:Neoplasm Staging; D009696:Nucleic Acids / Q000097:blood* / Q000302:isolation & purification* / Q000378:metabolism; D012313:RNA / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D002614:Chelating Agents; D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D004492:Edetic Acid; D003851:Deoxyribonucleases; D012260:Ribonucleases; D014357:Trypsin