Anti-sera raised in rabbits against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin.
Crotoxin, the principal neurotoxin in venom of the South American rattlesnakes Crotalus durissus terrificus and Crotalus durissus cascavella, contains a basic phospholipase A2 (PLA2) and an acidic protein, crotapotin. In this work, we examined the ability of rabbit anti-sera against crotoxin and its PLA2 subunit to neutralize the neurotoxicity of venom and crotoxin from C. d. cascavella in mouse phrenic nerve-diaphragm and chick biventer cervicis preparations. Immunoblotting showed that the anti-sera recognized C. d. cascavella crotoxin and PLA2. This was confirmed by ELISA, with both anti-sera having end-point dilutions of 3 x 10(-6). Anti-crotoxin serum neutralized the neuromuscular blockade in phrenic nerve-diaphragm muscle preparations at venom or crotoxin:anti-serum ratios of 1:2 and 1:3, respectively. Anti-PLA2 serum also neutralized this neuromuscular activity at a venom or crotoxin:anti-serum ratio of 1:1. In biventer cervicis preparations, the corresponding ratio for anti-crotoxin serum was 1:3 for venom and crotoxin, and 1:1 and 1:2 for anti-PLA2 serum. The neutralizing capacity of the sera in mouse preparations was comparable to that of commercial anti-serum raised against C. d. terrificus venom. These results show that anti-sera against crotoxin and PLA2 from C. d. cascavella venom neutralized the neuromuscular blockade induced by venom and crotoxin in both nerve-muscle preparations, with the anti-serum against crotoxin being slightly less potent than that against crotoxin.
Beghini
Daniela G
DG
Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13083-970 Campinas, SP, Brazil.
Hernandez-Oliveira
Saraguaci
S
Rodrigues-Simioni
Léa
L
Novello
José Camilo
JC
Hyslop
Stephen
S
Marangoni
Sérgio
S
D000704:Analysis of Variance; D000818:Animals; D000997:Antivenins / Q000276:immunology* / Q000494:pharmacology; D002645:Chickens; D003435:Crotalid Venoms / Q000201:enzymology / Q000276:immunology* / Q000633:toxicity; D003439:Crotoxin / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects; D009498:Neurotoxins / Q000276:immunology / Q000633:toxicity; D010741:Phospholipases A / Q000276:immunology*; D054467:Phospholipases A2; D011817:Rabbits
D000997:Antivenins; D003435:Crotalid Venoms; D009498:Neurotoxins; D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2