Structural and functional analogue of the active site of polysulfide reductase from Wolinella succinogenes.
Synthesis of [PPh4]2[Mo(SPh)2(S2C2(CN)2)2] (2) from [PPh4]2[MoO(S2C2(CN)2)2] (1) has been achieved to mimic the postulated [Mo(S)6] core of polysulfide reductase with two thiolates and two bis(ene-dithiolate) ligands. Compound 2 reacts with polysulfide to yield H2S, modeling the function of polysulfide reductase. The facile conversion of 2 back to 1 in moist solvent suggests that the interconversion of the [MoIV = O] and [MoIV - X] (X = O-Ser, S-Cys, Se-Cys) moieties might occur in the DMSO reductase class of enzymes under appropriate hydrophobic/hydrophilic conditions.
Nagarajan
Kowliki
K
Department of Chemistry, Indian Institute of Technology, Kanpur-208 016, India.
Joshi
Hemant K
HK
Chaudhury
Pradeep K
PK
Pal
Kuntal
K
Cooney
J Jon A
JJ
Enemark
John H
JH
Sarkar
Sabyasachi
S
D001665:Binding Sites; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D008982:Molybdenum / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D016968:Wolinella / Q000201:enzymology*
D009942:Organometallic Compounds; D008982:Molybdenum; D010088:Oxidoreductases; C074883:polysulfide reductase; D003545:Cysteine