Rapid neural differentiation of human cord blood-derived mesenchymal stem cells.
Human umbilical cord blood (UCB) contains hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs), both of which are regarded as valuable sources for cell transplantation and cell therapy. Adherent cells expressing MSCs-related antigens such as SH2, CD13, CD29, and ASMA, have been isolated from a mononuclear cell fraction of human UCB. Under proneurogenic conditions, these UCB-derived adherent cells rapidly assumed the morphology of multipolar neurons. Both immunofluorescence and RT-PCR analyses indicated that the expression of a number of neural markers including Tuj1, TrkA, GFAP and CNPases, was markedly elevated during this acute differentiation. The neurogenic potential of UCB-derived may facilitate stem cell therapeutic approaches to neurodegenerative diseases.
Jeong
Ju Ah
JA
Research Institute of Biotechnology, Histostem Co., 518-4 Taijul Bldg, Doonchun-dong, Kangdong-gu, Seoul 134-060, Korea.
Gang
Eun Ji
EJ
Hong
Seung Hyun
SH
Hwang
Soo Han
SH
Kim
Seong Whan
SW
Yang
Il Ho
IH
Ahn
Chiyoung
C
Han
Hoon
H
Kim
Hoeon
H
D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology