title: Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizigus marmoreus.
abstract: Antiproliferative activities of fractions of Hypsizigus marmoreus were examined using HepG2 cells in vitro. The methanol extract of H. marmoreus markedly induced antiproliferative activity, and an active compound from this mushroom was identified as hypsiziprenol A9. Hypsiziprenol A9 inhibited cell proliferation in a time- and concentration-dependent manner by up to 80% on HepG2 cells by inducing arrest of the G1 phase. Further investigation revealed that hypsiziprenol A9 decreased expression of phosphorylated retinoblastoma protein (ppRb), cyclin D1, and cyclin E in a dose-dependent manner. These results suggest that hypsiziprenol A9 can inhibit the growth of HepG2 cells through inducing G1 phase cell cycle arrest due to the inhibition of pRb phosphorylation.
authors.affiliation: Department of Food and Nutrition, Yeungnam University, Gyongsan 712-749, South Korea.
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Chang
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Gao
authors.initials: G
authors.lastname: Li
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Oh
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Joon-Tae
authors.initials: JT
authors.lastname: Bae
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Lee
authors.forename: Ok-Mi
authors.initials: OM
authors.lastname: Kim
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kozukue
authors.forename: Jae-Sook
authors.initials: JS
authors.lastname: Han
authors.forename: Masao
authors.initials: M
authors.lastname: Hirose
authors.forename: Kap-Rang
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D019938:Cyclin D1 / Q000096:biosynthesis; D019927:Cyclin E / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D019927:Cyclin E; D005233:Fatty Alcohols; D016160:Retinoblastoma Protein; C489023:hypsiziprenol A9; D019938:Cyclin D1