title: Lymphotoxin beta receptor signaling induces the chemokine CCL20 in intestinal epithelium.
abstract: OBJECTIVE
  The follicle-associated epithelium (FAE) that overlies Peyer's patches (PPs) exhibits distinct features compared with the adjacent villus epithelium. Besides the presence of antigen-sampling membranous M cells and the down-regulation of digestive functions, it constitutively expresses the chemokine CCL20. The mechanisms that induce FAE differentiation and CCL20 expression are poorly understood. The aim of this work was to test whether lymphotoxin beta receptor signaling (LTbetaR), which plays a central role in PPs' organogenesis, mediates CCL20 gene expression in intestinal epithelial cells.
  
  
  METHODS
  CCL20, lymphotoxin beta (LTbeta) and LTbetaR expression were monitored during embryonic development by in situ hybridization of mouse intestine. The human intestinal epithelial cell line T84 was used to study CCL20 expression following LTalpha(1)/beta(2) stimulation. In vivo CCL20 expression following agonistic anti-LTbetaR antibody treatment was studied by laser microdissection and quantitative RT-PCR.
  
  
  RESULTS
  CCL20 was expressed in the FAE before birth at the time when the first hematopoietic CD4(+)CD3(-) appeared in the PP anlage. LTbetaR was expressed in the epithelium during PP organogenesis, making it a putative target for LTalpha(1)beta(2)signals. In vitro, CCL20 was induced in T84 cells upon LTbetaR signaling, either using an agonistic ligand or anti-LTbeta receptor agonistic antibody. LTalpha(1)beta(2)-induced CCL20 expression was found to be NF-kappaB dependent. LTbetaR signaling up-regulated CCL20 expression in the small intestinal epithelium in vivo.
  
  
  CONCLUSIONS
  Our results show that LTbetaR signaling induces CCL20 expression in intestinal epithelial cells, suggesting that this pathway triggers constitutive production of CCL20 in the FAE.
authors.affiliation: Swiss Institute for Experimental Cancer Research, Lausanne Branch, Epalinges, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Rumbo
authors.forename: Frédéric
authors.initials: F
authors.lastname: Sierro
authors.forename: Nathalie
authors.initials: N
authors.lastname: Debard
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Kraehenbuhl
authors.forename: Daniela
authors.initials: D
authors.lastname: Finke
mesh_terms: D000818:Animals; D002460:Cell Line; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000096:biosynthesis*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D053259:Lymphotoxin beta Receptor; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010581:Peyer's Patches / Q000378:metabolism / Q000473:pathology; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: C108355:CCL20 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; C505697:LTBR protein, human; C505699:Ltbr protein, mouse; D053259:Lymphotoxin beta Receptor; D019402:Macrophage Inflammatory Proteins; D018124:Receptors, Tumor Necrosis Factor