title: Increased plasma HDL cholesterol levels and biliary cholesterol excretion in hamster by LCAT overexpression.
abstract: Lecithin cholesterol acyltransferase (LCAT) is a key enzyme in the metabolism of high density lipoprotein (HDL), which has been found inversely correlated with atherosclerosis. Adenovirus mediated overexpression of human LCAT (hLCAT) in hamsters resulted in increased levels of plasma total cholesterol, HDL cholesterol, phospholipids and enlarged particle size of HDL. It also increased cholesterol and total bile acid concentrations in bile. Hepatic mRNA level of cholesterol 7alpha-hydroxylase increased 2.7-fold in hamsters. However, such effects were not observed in mice in a parallel experiment. This study suggests that overexpression of hLCAT in hamsters facilitated reverse cholesterol transport. Similar metabolic changes in humans might modify atherogenic risk.
authors.affiliation: Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiology, Ministry of Education, Peking University, 38 Xue Yuan Road, Beijing 100083, PR China.
authors.forename: Ai-Hong
authors.initials: AH
authors.lastname: Zhang
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Jiang-Lin
authors.initials: JL
authors.lastname: Fan
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Tie-Qiang
authors.initials: TQ
authors.lastname: Zhao
authors.forename: George
authors.initials: G
authors.lastname: Liu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001646:Bile / Q000378:metabolism*; D001692:Biological Transport; D015153:Blotting, Western; D002784:Cholesterol / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D050356:Lipid Metabolism; D008075:Lipoproteins, HDL / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D012333:RNA, Messenger; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase