title: Sample displacement chromatography of Atlantic Salmon (Salmo salar) thrombin.
abstract: A modified method of sample displacement chromatography (SDC) was used to purify active salmon thrombin on a heparin-coupled matrix to near homogeneity in milligram amounts from 117 ml plasma. This was achieved by combining a low-pressure multi-column affinity chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification. Some characteristics of salmon thrombin are presented.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Chr.M.Falsensgate 1, P.O. Box 5040, N-1432 As, Norway. even.manseth@ikbm.nlh.no
authors.forename: Even
authors.initials: E
authors.lastname: Manseth
authors.forename: Per O
authors.initials: PO
authors.lastname: Skjervold
authors.forename: Ragnar
authors.initials: R
authors.lastname: Flengsrud
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001550:Benzamidines / Q000737:chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D011516:Prothrombin / Q000737:chemistry; D012474:Salmon; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013917:Thrombin / Q000032:analysis / Q000737:chemistry*; D014426:Turtles
chemical_list: D001550:Benzamidines; C037405:heparin-sepharose; D011516:Prothrombin; D012685:Sepharose; D013917:Thrombin; C032157:benzamidine