1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol.
A crtD (1-HO carotenoid 3,4-dehydrogenase gene) homolog from marine bacterium strain P99-3 included in the gene cluster for the biosynthesis of myxol (3',4'-didehydro-1',2'-dihydro-beta, psi-carotene-3,1',2'-triol) was functionally identified. The P99-3 CrtD was phylogenetically distant from the other CrtDs. A catalytic feature was its high activity for the monocyclic carotenoid conversion: 1'-HO-torulene (3',4'-didehydro-1',2'-dihydro- beta, psi-caroten-1'-ol) was prominently formed from 1'-HO-gamma-carotene (1',2'-dihydro-beta, psi-caroten-1'-ol) in Escherichia coli with P99-3 CrtD, indicating that this enzyme has been highly adapted to myxol biosynthesis. This unique type of crtD is a valuable tool for obtaining 1'-HO-3',4'-didehydro monocyclic carotenoids in a heterologous carotenoid production system.
Teramoto
Maki
M
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi-shi, Iwate 026-0001, Japan. maki.teramoto@mbio.jp
Rählert
Nina
N
Misawa
Norihiko
N
Sandmann
Gerhard
G
D001419:Bacteria / Q000201:enzymology*; D002338:Carotenoids / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D008956:Models, Chemical; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics*; D010802:Phylogeny; D013379:Substrate Specificity; D013997:Time Factors; D024341:Xanthophylls / Q000737:chemistry*
D024341:Xanthophylls; C488895:myxol; D002338:Carotenoids; D010088:Oxidoreductases; C109407:hydroxyneurosporene desaturase