title: CD10 immunohistochemical staining enhances the histological detection of endometriosis.
abstract: OBJECTIVE
  To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Tertiary care government research hospital.
  
  
  METHODS
  Thirty-one women with chronic pelvic pain.
  
  
  METHODS
  Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised.
  
  
  METHODS
  Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens.
  
  
  RESULTS
  In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45% vs. 35%). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis.
  
  
  CONCLUSIONS
  The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.
authors.affiliation: Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892-1583, USA. naharic@mail.nih.gov
authors.forename: Clariss
authors.initials: C
authors.lastname: Potlog-Nahari
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Feldman
authors.forename: Pamela
authors.initials: P
authors.lastname: Stratton
authors.forename: Deloris E
authors.initials: DE
authors.lastname: Koziol
authors.forename: James
authors.initials: J
authors.lastname: Segars
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Merino
authors.forename: Lynnette K
authors.initials: LK
authors.lastname: Nieman
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004396:Coloring Agents; D003937:Diagnosis, Differential; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005260:Female; D005456:Fluorescent Dyes; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D015260:Neprilysin; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin