[Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis].
OBJECTIVE
To determine the content of dexamethasone sodium phosphate in Fuyankang cream, a preparation for treatment of various skin conditions.


METHODS
Dexamethasone sodium phosphate content in Fuyankang cream was determined using high-performance capillary electrophoresis (HPCE) at the detection wavelength of 254 nm, under the optimal condition achieved with a fused-silica capillary tube (60 cm x 75 microm) and 100 mmol/L sodium tetraborate buffer (pH 9.2) at a constant voltage of 25 kV with a sampling time of 10 s at 25 degrees Celsius;.


RESULTS
The calibration curve displayed good linear relationship within dexamethasone sodium phosphate concentration range of 20 to 100 microg/ ml r=0.999 8 . The average recovery of dexamethasone sodium phosphate was 99.75% n=5, RSD=1.03% .


CONCLUSIONS
HPCE is simple, quick and sensitive in determination of dexamethasone sodium phosphate content in Fuyankang cream.
Guo
Dan
D
Department of Pharmacy, Nanfang Hospital, Institute of Pharmaceutical Sciences, First Military Medical University, Guangzhou 510515, China.
Chen
Na-na
NN
Yang
Xi-xiao
XX
Hou
Lian-bing
LB
D002138:Calibration; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000032:analysis*; D004355:Drug Stability; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D009824:Ointments / Q000032:analysis
D009824:Ointments; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone