title: Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.
abstract: Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects.
authors.affiliation: Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli Bologna, Bologna.
authors.forename: B
authors.initials: B
authors.lastname: Grigolo
authors.forename: G
authors.initials: G
authors.lastname: Lisignoli
authors.forename: A
authors.initials: A
authors.lastname: Piacentini
authors.forename: M
authors.initials: M
authors.lastname: Fiorini
authors.forename: L
authors.initials: L
authors.lastname: Roseti
authors.forename: L
authors.initials: L
authors.lastname: De Franceschi
authors.forename: E
authors.initials: E
authors.lastname: Tognana
authors.forename: A
authors.initials: A
authors.lastname: Pavesio
authors.forename: A
authors.initials: A
authors.lastname: Facchini
mesh_terms: D000293:Adolescent; D000328:Adult; D002356:Cartilage / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D023822:Tissue Engineering / Q000379:methods
chemical_list: C086358:hyaluronic acid benzyl ester; D006820:Hyaluronic Acid