title: Characterization of dehydration behavior of untreated and pulverized creatine monohydrate powders.
abstract: Creatine, which is well known as an important substance for muscular activity, is synthesized from amino acids such as glycine, arginine and ornithine in liver and kidney. It then accumulates in skeletal muscle as creatine phosphoric acid. The aim of this study was to understand the dehydration behavior of untreated and pulverized creatine monohydrate at various temperatures. The removal of crystal water was investigated by using differential scanning calorimetry (DSC), X-ray powder diffraction and scanning electron microscopy (SEM). The X-ray diffraction pattern of untreated and pulverized creatine monohydrate agreed with reported data for creatine monohydrate. However, the diffraction peaks of the (100), (200) and (300) planes of pulverized creatine monohydrate were much stronger than those of untreated creatine monohydrate. On the other hand, the diffraction peaks of the (012) and (013) planes of untreated creatine monohydrate were much stronger than those of pulverized creatine monohydrate. The dehydration of untreated and pulverized creatine monohydrate was investigated at various storage temperatures, and the results indicated that untreated and pulverized creatine monohydrate were transformed into the anhydrate at more than 30 degrees C. After dehydration, the particles of untreated and pulverized creatine anhydrate had many cracks. The dehydration kinetics of untreated and pulverized creatine monohydrate were analyzed by the Hancock-Sharp equation on the basis of the isothermal DSC data. The dehydrations of untreated and pulverized creatine monohydrate both followed a zero-order mechanism (Polany-Winger equation). However, the transition rate constant, calculated from the slope of the straight line, was about 2.2-7.7 times higher for pulverized creatine monohydrate than for untreated creatine monohydrate. The Arrhenius plots (natural logarithm of the dehydration rate constant versus the reciprocal of absolute temperature) of the isothermal DSC data for untreated and pulverized creatine monohydrate were linear. The activation energies of dehydration in the 40-60 degrees C range for untreated and pulverized creatine monohydrate were 15.02 and 10.1 kJ/mol, respectively. Dehydration of untreated creatine monohydrate had a pronounced effect on the particle size of the powder. Compared with pulverized creatine monohydrate, the particle size of untreated creatine monohydrate was significantly decreased by dehydration.
authors.affiliation: Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd., 1624 Shimokotachi, Kodacho, Takatagun, Hiroshima 739-1195, Japan. sakata_y@wakunaga.co.jp
authors.forename: Yukoh
authors.initials: Y
authors.lastname: Sakata
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Shiraishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D002152:Calorimetry, Differential Scanning; D003401:Creatine / Q000737:chemistry*; D003890:Desiccation; D007700:Kinetics; D011208:Powders / Q000737:chemistry; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D011208:Powders; D003401:Creatine