title: Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas.
abstract: Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of atrazine, diuron and isoproturon. The results showed a strong differential expression of FMO-2 and MAO A that was both tissue-specific as well as time- and treatment-dependent. It was also clearly demonstrated that the transcription levels of MAO A (generally considered a constitutive enzyme without external regulation) were induced by hydrocarbons and pesticides in digestive gland and inhibited by pesticides in gill tissue. Furthermore, the transcription levels of FMO-2 and MAO A mRNA in digestive gland might be useful as a marker of hydrocarbon or pesticide exposure in monitoring programs.
authors.affiliation: Station Méditerranéenne de l'Environnement Littoral, UMR CNRS-IFREMER 5171 "Génome, Populations, Interactions, Adaptation", 1 Quai de la Daurade, 34200 Sète, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Boutet
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tanguy
authors.forename: Dario
authors.initials: D
authors.lastname: Moraga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001711:Biotransformation; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010125:Ostreidae / Q000201:enzymology / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D015262:Xenobiotics / Q000493:pharmacokinetics*
chemical_list: D018076:DNA, Complementary; D015262:Xenobiotics; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming); D008995:Monoamine Oxidase