title: Expression in bladder transitional cell carcinoma by real-time quantitative reverse transcription polymerase chain reaction array of 65 genes at the tumor suppressor locus 9q34.1-2: identification of 5 candidates tumor suppressor genes.
abstract: Frequent deletions on 9q34.1-2 were reported in bladder transitional cell carcinoma. High deletion mapping studies delimited a critical interval between markers D9S61 and D9S66, which is highly susceptible to contain a tumor suppressor gene. Expression level of the 65 genes localized in this region was analyzed by real-time quantitative RT-PCR, comparing tumor to normal urothelium. Five genes exhibited a significantly reduced expression level: C9orf9, KIAA0625, ABL1, LAMC3 and KIAA1857-netrin-G2, which exhibited the most significant downregulation (p=0.0007). KIAA1857-netrin-G2 belongs to the netrins and might then be a tumor suppressor gene in bladder cancer, as netrin1 receptor DCC has been implicated in tumorigenesis.
authors.affiliation: UroGene, Génopole, Evry, France.
authors.forename: Najla
authors.initials: N
authors.lastname: Amira
authors.forename: Géraldine
authors.initials: G
authors.lastname: Cancel-Tassin
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bernardini
authors.forename: Béatrix
authors.initials: B
authors.lastname: Cochand-Priollet
authors.forename: Hugues
authors.initials: H
authors.lastname: Bittard
authors.forename: Philippe
authors.initials: P
authors.lastname: Mangin
authors.forename: Georges
authors.initials: G
authors.lastname: Fournier
authors.forename: Alain
authors.initials: A
authors.lastname: Latil
authors.forename: Olivier
authors.initials: O
authors.lastname: Cussenot
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000235:genetics*