The ANTHER INDEHISCENCE1 gene encoding a single MYB domain protein is involved in anther development in rice.
Using a two-element iAc/Ds transposon-tagging system, we identified a rice (Oryza sativa L. cv Nipponbare) recessive mutant, anther indehiscence1 (aid1), showing partial to complete spikelet sterility. Spikelets of the aid1 mutant could be classified into three types based on the viability of pollen grains and the extent of anther dehiscence. Type 1 spikelets (approximately 25%) were sterile due to a failure in accumulation of starch in pollen grains. Type 2 spikelets (approximately 55%) had viable pollen grains, but anthers failed to dehisce and/or synchronize with anthesis due to failure in septum degradation and stomium breakage, resulting in sterility. Type 3 spikelets (approximately 20%) had normal fertility. In addition, aid1 mutant plants had fewer tillers and flowered 10 to 15 d later than the wild type. The Ds insertion responsible for the aid1 mutation was mapped within the coding region of the AID1 gene on chromosome 6, which is predicted to encode a novel protein of 426 amino acids with a single MYB domain. The MYB domain of AID1 is closely related to that of the telomere-binding proteins of human, mouse, and Arabidopsis, and of single MYB domain transcriptional regulators in plants such as PcMYB1 and ZmIBP1. AID1 was expressed in both the leaves and panicles of wild-type plants, but not in mutant plants.
Zhu
Qian-Hao
QH
CSIRO Plant Industry, Canberra, Australian Capital Territories 2601, Australia.
Ramm
Kerrie
K
Shivakkumar
Ramani
R
Dennis
Elizabeth S
ES
Upadhyaya
Narayana M
NM
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C511988:ATR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C511989:CPC protein, Arabidopsis; D017931:DNA Primers; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb