title: Identification of a novel brain-specific and Reelin-regulated gene that encodes a protein colocalized with synapsin.
abstract: We carried out a screening of genes that are differentially expressed in normal mice and reeler mutants and are characterized by abnormal neuronal migration and neurite deployment due to defective Reelin signalling. A novel gene, provisionally named C61, was overexpressed in Reelin-deficient embryonic mouse brain RNA. C61 encodes a 3.7 kb mRNA that is brain specific and developmentally regulated, with predominant expression in differentiating neurons. The predicted protein is 664 amino acids long, and contains LAG1 and Ezrin/Radixin/Moesin-Myosin-Filament motifs, suggesting that it may function as an intracellular adaptor. From E14.5 to birth, C61 was highly expressed in all neuronal differentiation fields, with the highest signal in the telencephalic cortical plate and mitral cells in the olfactory bulb. When expressed as a GFP fusion protein in transfected non-neuronal cells and primary neurons, this protein localizes, respectively, to the nuclear membrane or axonal outgrowths, indicating a function in axonal traffic or signalling.
authors.affiliation: Unité de Neurobiologie, Facultés Universitaires ND de la Paix, Namur, Belgium.
authors.forename: Anelia
authors.initials: A
authors.lastname: Kuvbachieva
authors.forename: Aude-Marie
authors.initials: AM
authors.lastname: Bestel
authors.forename: Fadel
authors.initials: F
authors.lastname: Tissir
authors.forename: Ismahane
authors.initials: I
authors.lastname: Maloum
authors.forename: Fabien
authors.initials: F
authors.lastname: Guimiot
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ramoz
authors.forename: Francine
authors.initials: F
authors.lastname: Bourgeois
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Moalic
authors.forename: André M
authors.initials: AM
authors.lastname: Goffinet
authors.forename: Michel
authors.initials: M
authors.lastname: Simonneau
mesh_terms: D020816:Amino Acid Motifs / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D017173:Caenorhabditis elegans; D015816:Cell Adhesion Molecules, Neuronal / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D003001:Cloning, Molecular; D004330:Drosophila; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D016326:Extracellular Matrix Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008818:Mice, Neurologic Mutants; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins; D025581:Neurofibromin 2 / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D000091042:Reelin Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012697:Serine Endopeptidases; D016704:Synapsins / Q000378:metabolism*; D014162:Transfection; D014404:Tubulin / Q000378:metabolism; D015027:Zebrafish
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D008164:Luminescent Proteins; C490073:MACO1 protein, human; C490074:Maco1 protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D025581:Neurofibromin 2; D012333:RNA, Messenger; D000091042:Reelin Protein; D016704:Synapsins; D014404:Tubulin; C462941:beta3 tubulin, mouse; D049452:Green Fluorescent Proteins; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases