title: Multiplexed sandwich assays in microarray format.
abstract: Antibody arrays have evolved into powerful tools for quantifying proteins and qualifying their state of activation in complex biological samples. This level of analysis holds tremendous promise as part of a diagnostic or prognostic platform. In particular, multiplex sandwich ELISA assays performed with microarrays in the wells of multi-well plates enable high-throughput analysis of multiple samples with multivariate readouts. Here, we compare and review recent advances in antibody microarray technology and describe its promises towards the systematic analysis of complex biological samples, ranging from profiling patient sera to studying intracellular signaling.
authors.affiliation: Merrimack Pharmaceuticals, 101 Binney Street, Cambridge, MA 02142, USA. unielsen@merrimackpharma.com
authors.forename: Ulrik B
authors.initials: UB
authors.lastname: Nielsen
authors.forename: Bernhard H
authors.initials: BH
authors.lastname: Geierstanger
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D040081:Protein Array Analysis / Q000379:methods*; D015398:Signal Transduction / Q000276:immunology
chemical_list: D000906:Antibodies