title: Presence of translation elongation factor-1A (eEF1A) in the excitatory postsynaptic density of rat cerebral cortex.
abstract: The postsynaptic density (PSD) is a proteinaceous cellular structure that is specialized for postsynaptic signal transduction. Here, we show that eukaryotic translation factor-1A (eEF1A; formerly known as eEF-1alpha) is associated with the excitatory PSD in rat forebrain. Immunoblot analysis showed that eEF1A in the PSD fraction is enriched over homogenate. Salt (1.0M NaCl), but not non-ionic detergents such as Triton X-100 (1.0%) and n-octyl glucoside (1.0%), could dissociate eEF1A from the PSD core. In cultured cortical neurons, eEF1A was colocalized with postsynaptic markers (PSD95 and SynGAPalpha), but not with a presynaptic marker (synaptophysin). These results indicate that eEF1A is present in the PSD of the excitatory synapses.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Cho
authors.forename: Jae-Seob
authors.initials: JS
authors.lastname: Jung
authors.forename: Bok Hyun
authors.initials: BH
authors.lastname: Ko
authors.forename: IngNyol
authors.initials: I
authors.lastname: Jin
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D015151:Immunoblotting; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism
chemical_list: D020648:Peptide Elongation Factor 1