Isolation and characterization of the CD133+ precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
A fast and effective method to enrich large number of neural precursors from the ventricular zone of human fetus by magnetic affinity cell sorting (MACS) is reported. After incubation with phycoerythrin (PE)-conjugated anti-CD133 antibodies and anti-PE magnetic beads followed by one cycle of MACS, CD133(+) cells were harvested at 85% purity as confirmed by flow-cytometry and immunocytochemistry. In contrast to CD133(-) cells, these CD133(+) cells initiated primary and secondary neurospheres in culture, and the progeny of sorted cells could be differentiated into both neurons and glia, indicating that these highly enriched cells are capable of self-renewal and multi-lineage potential.
Yu
S
S
Beijing Institute for Neuroscience and Beijing Center for Neural Regeneration and Repairing, Capital University of Medical Sciences, Beijing 100054, P.R. China.
Zhang
J Z
JZ
Zhao
C L
CL
Zhang
H Y
HY
Xu
Q
Q
D000071916:AC133 Antigen; D015703:Antigens, CD; D001921:Brain / Q000196:embryology*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008280:Magnetics; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification*; D013997:Time Factors
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides