[DNA extraction using Chelex resin for oncogenic amplification analysis in head and neck tumours].
DNA extraction from tissues can be the most laborious and complex step in amplifying DNA by PCR when phenol-choroform procedure is used. We compare this lengthy, slow and expensive extraction method with other two based in the use of Chelex-100 resin. This chelating resin has been applied for extracting DNA from different tissues to use with the PCR. These procedures are simple, rapid and do not require multiple steps. In this study we compared DNA extraction from 30 head and neck squamous cell carcinomas (HNSCC) using organic solvent precipitation, Chelex 100 resin with and without proteinase K pretreatment. The results show that proteinase K-Chelex 100 procedure is as efficient as the phenol-chloroform one.
García González
L A
LA
Servicio de ORL, Hospital Carmen y Severo Ochoa, Asturias. lgarciag@hcso.sespa.es
Rodrigo Tapia
J P
JP
Sánchez Lazo
P
P
Ramos
S
S
Suárez Nieto
C
C
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002614:Chelating Agents; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011137:Polystyrenes; D011145:Polyvinyls
D002614:Chelating Agents; D004273:DNA, Neoplasm; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex