DFU, a selective COX-2 inhibitor, suppresses MCF-7 xenograft tumor growth in mice.
Cyclooxygenase-2 (COX-2) inhibitors are regarded as potentially important in strategies for cancer treatment. however, the precise mechanisms of these anti-inflammatory drugs as anti-cancer therapy are still unknown. In this study, we examined the effect of DFU both in vitro on MCF-7 cell growth, as well as in vivo on tumor growth produced by MCF-7 cell injection in mice. DFU has growth inhibitory effects on tumor growth in mice compared to the control group. We examined the tumor tissues for apoptosis and angiogenesis by immunostaining. Apoptosis was detected only in the treatment group. DFU treatment also resulted in the inhibition of angiogenesis, as well as decreased COX-2 expression. Results of this study suggest that inhibitory effects of DFU might be COX-2 dependent.
Matsumoto
Gaku
G
Department of Surgery, Tokyo Metropolitan Komagome Hospital, Honkomagome, Bunkyo-ku, Tokyo 113-8677, Japan. gaku0508@ruby.ocn.ne.jp
Rahman
Mohammad Atiqur
MA
Muta
Mariko
M
Nakamura
Tadahiko
T
Bando
Hiroko
H
Saji
Shigehira
S
Tsuruta
Koji
K
Okamoto
Atsutake
A
Toi
Masakazu
M
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051546:Cyclooxygenase 2; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases; D013997:Time Factors
C106876:5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5H)-furanone; D004791:Enzyme Inhibitors; D005663:Furans; D007527:Isoenzymes; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases