An innovative technique for inoculating recombinant baculovirus into the silkworm Bombyx mori using lipofectin.
The insect baculovirus expression system is one of the most effective eukaryotic expression systems known, and has been widely used to produce numerous recombinant proteins. The current traditional inoculation method consists of injecting recombinant baculovirus directly into insects, thus causing potential contamination to the environment due to virus diffusion during the inoculation process. In the present experiment, we directly introduced baculovirus DNA into silkworms using a cationic lipofectin reagent instead of directly injecting the virus. This new method produced the same infection results as the traditional method. A new safe infection technique without direct use of the virus was thus developed.
Wu
Xiaofeng
X
College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, PR China. wuxiaofeng@zju.edu.cn
Cao
Cuiping
C
Kumar
V Shyam
VS
Cui
Weizheng
W
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000302:isolation & purification; D012831:Bombyx / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019476:Insect Proteins; D007814:Larva / Q000235:genetics / Q000378:metabolism / Q000821:virology; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D019476:Insect Proteins; D010714:Phosphatidylethanolamines; D011994:Recombinant Proteins; C020888:1,2-dielaidoylphosphatidylethanolamine