title: Screening for dihydropyridine calcium channel blockers in plasma by automated solid-phase extraction and liquid chromatography/tandem mass spectrometry.
abstract: A liquid chromatographic/tandem mass spectrometric (LC/MS/MS) screening method was developed for the screening of 11 calcium channel blockers of the 1,4-dihydropyridine type in plasma samples for forensic and clinical cases. Plasma samples were extracted by automated solid-phase extraction. Analysis was performed using a reversed-phase C(18) column, gradient elution and a triple-quadrupole mass spectrometer with TurboIonSpray source in positive mode and multiple reaction monitoring. This method was found to be selective and sensitive for the detection of the target compounds at their therapeutic plasma concentrations.
authors.affiliation: Institute of Legal Medicine, Forensic Toxicology, University Hospital, Albertstrasse 9, D-79104 Freiburg, Germany. muellerc@ukl.uni-freiburg.de
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Mueller
authors.forename: Ana B Baranda
authors.initials: AB
authors.lastname: González
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weinmann
mesh_terms: D001331:Automation; D002121:Calcium Channel Blockers / Q000032:analysis* / Q000737:chemistry; D015220:Calcium Channels / Q000378:metabolism; D002853:Chromatography, Liquid; D004095:Dihydropyridines / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004095:Dihydropyridines; C038806:1,4-dihydropyridine