Research on solid-state fermentation on rice chaff with a microbial consortium.
A microbial consortium of Trichoderma reesei AS3.3711, Aspergillus niger 3.316 and Saccharomyces cerevisiaes AS2.399 was constructed to decomposed rice chaff on the basis of the characters of each microorganism and the mechanism of cellulases. In this experiment, rice chaff was pretreated before fermentation with NaOH so that the lignin structure of rice chaff was degraded and hemicellulose was dissolved partly, which remove the protection of lignin and hemicellulose on cellulose and demolish its special crystal structure. After pretreatment, rice chaff can be degraded more easily with the microbial consortium. The optimal technical paths and technological methods were achieved for intenerating rice chaff with the microbial consortium perfectly through orthogonal experiment. According to the technological methods, some experiments were done at 30 degrees C with pH 4.5. It was found that the highest filter paper enzyme activity (FPA) was 5.64 U/g and the ratio of cellulose degradation (RCD) was 28.05%.
Yang
Y H
YH
State Key Laboratory of Biomechanics and Tissue Engineering, Institute of Bioengineering, Chongqing University, Chongqing 400044, China.
Wang
B C
BC
Wang
Q H
QH
Xiang
L J
LJ
Duan
C R
CR
D001234:Aspergillus niger / Q000378:metabolism; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D018360:Crystallography, X-Ray; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008031:Lignin / Q000737:chemistry; D012275:Oryza / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000378:metabolism
D011134:Polysaccharides; D012965:Sodium Chloride; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase