title: Diagnostic dose of synergized d-phenothrin for insecticide susceptibility testing by bottle bioassay.
abstract: The diagnostic dose of d-phenothrin synergized 1:1 with piperonyl butoxide for testing insecticide susceptibility of mosquitoes by bottle bioassay is reported for 2 mosquito species, Culex quinquefasciatus and Ochlerotatus taeniorhynchus. The diagnostic dose was defined as 2 times the 95% lethal concentration (LC95). LC50, LC90, and LC95 were estimated by probit analysis of dose response data. Procedures for diluting the commercial-grade off-the-shelf pesticide in acetone, treating the bottles, and calculating baseline data for insecticide-susceptible mosquito populations are described. The advantages and disadvantages of testing off-the-shelf commercial-grade pesticides that are maintained on premises by mosquito control programs, in contrast to using reagent-grade chemicals purchased from a chemical supply house, are also discussed. Data obtained by this method can be invaluable in making timely management decisions about the choice of pesticides in a control program.
authors.affiliation: Florida A&M University, John A. Mulrennan, Sr., Public Health Entomology Research and Education Center, College of Engineering Sciences, Technology and Agriculture, 4000 Frankford Avenue, Panama City, FL 32405, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Petersen
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Floore
authors.forename: William G
authors.initials: WG
authors.lastname: Brogdon
mesh_terms: D000818:Animals; D001681:Biological Assay; D003465:Culex; D004305:Dose-Response Relationship, Drug; D007305:Insecticide Resistance; D007306:Insecticides; D044969:Ochlerotatus; D010882:Piperonyl Butoxide; D011722:Pyrethrins; D013997:Time Factors
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C006166:phenothrin; D010882:Piperonyl Butoxide