Sample displacement chromatography of Atlantic Salmon (Salmo salar) thrombin.
A modified method of sample displacement chromatography (SDC) was used to purify active salmon thrombin on a heparin-coupled matrix to near homogeneity in milligram amounts from 117 ml plasma. This was achieved by combining a low-pressure multi-column affinity chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification. Some characteristics of salmon thrombin are presented.
Manseth
Even
E
Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Chr.M.Falsensgate 1, P.O. Box 5040, N-1432 As, Norway. even.manseth@ikbm.nlh.no
Skjervold
Per O
PO
Flengsrud
Ragnar
R
D000595:Amino Acid Sequence; D000818:Animals; D001550:Benzamidines / Q000737:chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D011516:Prothrombin / Q000737:chemistry; D012474:Salmon; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013917:Thrombin / Q000032:analysis / Q000737:chemistry*; D014426:Turtles
D001550:Benzamidines; C037405:heparin-sepharose; D011516:Prothrombin; D012685:Sepharose; D013917:Thrombin; C032157:benzamidine