title: High-level gene expression in Lactobacillus plantarum using a pheromone-regulated bacteriocin promoter.
abstract: OBJECTIVE
  To use promoters and regulatory genes involved in the production of the bacteriocin sakacin P to obtain high-level regulated gene expression in Lactobacillus plantarum.
  
  
  RESULTS
  In a plasmid containing all three operons naturally involved in sakacin P production, the genes encoding sakacin P and its immunity protein were replaced by the aminopeptidase N gene from Lactococcus lactis (pepN) or the beta-glucuronidase gene from Escherichia coli (gusA). The new genes were precisely fused to the start codon of the sakacin P gene and the stop codon of the immunity gene. This set-up permitted regulated (external pheromone controlled) overexpression of both reporter genes in L. plantarum NC8. For PepN, production levels amounted to as much as 40% of total cellular protein.
  
  
  CONCLUSIONS
  Promoters and regulatory genes involved in production of sakacin P are suitable for establishing inducible high-level gene expression in L. plantarum.
  
  
  CONCLUSIONS
  This study describes a system for controllable gene expression in lactobacilli, giving some of the highest expression levels reported so far in this genus.
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, As, Norway.
authors.forename: G
authors.initials: G
authors.lastname: Mathiesen
authors.forename: E
authors.initials: E
authors.lastname: Sørvig
authors.forename: J
authors.initials: J
authors.lastname: Blatny
authors.forename: K
authors.initials: K
authors.lastname: Naterstad
authors.forename: L
authors.initials: L
authors.lastname: Axelsson
authors.forename: V G H
authors.initials: VG
authors.lastname: Eijsink
mesh_terms: D003086:Bacteriocin Plasmids / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics*; D018826:CD13 Antigens / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005966:Glucuronidase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics; D010675:Pheromones / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D001430:Bacteriocins; D010675:Pheromones; D011993:Recombinant Fusion Proteins; C087217:sakacin P protein, Lactobacillus; D005966:Glucuronidase; D018826:CD13 Antigens