Mercaptoheterocyclic ligands grafted on a poly(ethylene vinyl alcohol) membrane for the purification of immunoglobulin G in a salt independent thiophilic chromatography.
In this study, we attempted a limited combinatorial approach for designing affinity ligands based on mercaptoheterocyclic components. The template, divinyl sulfone structure (DVS), which was grafted on poly(ethylene vinyl alcohol) (PEVA) hollow fiber membrane, has served for the tethering of different heterocyclic compounds as pyridine, imidazole, purine and pyrimidine rings. Their ability to adsorb specifically IgG in a salt independent manner out of pure IgG solution, mixture of IgG/albumin and human plasma was demonstrated. Mercapto methyl imidazole (MMI) has shown the best adsorption of IgG in terms of binding capacity. No subclass discrimination was observed on all tested ligands except for mercapto methyl pyrimidine where the major IgG subclass adsorbed was IgG3. MMI gave an IgG binding capacity of 100 microg/cm2 of hollow fiber membrane surface area.
Coffinier
Yannick
Y
Laboratoire d'Interactions Moléculaires et des Technologies de Séparation (LIMTechS), Centre de Recherche de Royallieu, Université de Technologies de Compiègne (UTC), BP 20.529, 60205 Compiègne Cedex, France.
Vijayalakshmi
Mookambeswaran A
MA
D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008024:Ligands; D008567:Membranes, Artificial; D011145:Polyvinyls / Q000737:chemistry*
D006571:Heterocyclic Compounds; D007074:Immunoglobulin G; D008024:Ligands; D008567:Membranes, Artificial; D011145:Polyvinyls; C020320:ethylene-vinyl alcohol copolymer