title: Identification of a novel apolipoprotein, ApoN, in ovarian follicular fluid.
abstract: A novel apolipoprotein, designated ApoN, has been identified in bovine ovarian follicular fluid using chromatographic purification methods, amino acid sequence analysis, molecular biology, and bioinformatics. The apolipoprotein is a hydrophobic 12-kDa protein processed from the C terminus of a 29-kDa precursor expressed in a number of tissues, including the ovary, testis, the anterior chamber of the eye, skeletal muscle, uterus, and liver. Bovine, porcine, and murine ApoN display significant homology at the amino acid level across the entire precursor sequence. Surprisingly, there appears to be no orthologous protein in the human, although an APON-like pseudogene is found on chromosome 12. The N-terminal fragment of the ApoN precursor shows significant homology with the N-terminal sequence of the precursor of the cholesterol transport regulatory protein ApoF, but the corresponding C-terminal sequences of ApoN and ApoF possess no homology. ApoN is present in the high-density lipoprotein fraction of bovine serum and both the high-density lipoprotein and low-density lipoprotein fractions of bovine follicular fluid and is found in several tissues that are associated with local immunological privilege. These data suggest that ApoN may play a role in steroidogenesis and/or immunoregulation in the gonads of nonhuman species, as well as similar roles in other tissues.
authors.affiliation: The Centre for Molecular Reproduction and Development, Monash Institute of Reproduction and Development, 27-31 Wright Street, Clayton 3168, Melbourne, Victoria, Australia. moira.obryan@med.monash.edu.au
authors.forename: Moira K
authors.initials: MK
authors.lastname: O'Bryan
authors.forename: Lynda M
authors.initials: LM
authors.lastname: Foulds
authors.forename: James F
authors.initials: JF
authors.lastname: Cannon
authors.forename: Wendy R
authors.initials: WR
authors.lastname: Winnall
authors.forename: Julie A
authors.initials: JA
authors.lastname: Muir
authors.forename: Kim
authors.initials: K
authors.lastname: Sebire
authors.forename: A Ian
authors.initials: AI
authors.lastname: Smith
authors.forename: Hooi-Hong
authors.initials: HH
authors.lastname: Keah
authors.forename: Milton T W
authors.initials: MT
authors.lastname: Hearn
authors.forename: David M
authors.initials: DM
authors.lastname: de Kretser
authors.forename: Mark P
authors.initials: MP
authors.lastname: Hedger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001053:Apolipoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010053:Ovary / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
chemical_list: D000906:Antibodies; D001053:Apolipoproteins; D018076:DNA, Complementary