Using skin models to assess the effects of a protection cream on skin barrier function.
BACKGROUND
There is a basic necessity to understand the mechanisms of the protective effects of emulsions. This would promote the development of protective cosmetics and therefore improve the prevention and treatment of occupational skin diseases. However, for such studies, no reliable skin model is available.


OBJECTIVE
An in vitro skin model test was developed to evaluate the protective mechanism of cosmetic ingredients.


METHODS
The efficacy of three products was assessed by an in vivo test (Repetitive Occlusive Irritation Test) and then 3-dimensional skin model tests were carried out.


RESULTS
In vivo test results demonstrate that the best protection against sodium dodecyl sulphate is offered by a multiple emulsion. In the case of a skin model test, sodium dodecyl sulphate led to cell damage, an increase in pro-inflammatory markers and some barrier lipids. The multiple emulsion increased the content of skin lipids, without inducing irritation or cell death.


CONCLUSIONS
Skin models react similarly to sodium dodecyl sulphate compared to human skin and therefore they are suitable to study barrier repair after sodium dodecyl sulphate damage. It is likely that the superior protective effect of the multiple emulsion in vivo is based on the increased amount of skin barrier lipids.
zur Mühlen
A
A
Degussa, Stoko Skin Care, Laboratory for Toxicology and Ecology, Krefeld, Germany. Annette.zur_muehlen@degussa.com
Klotz
A
A
Weimans
S
S
Veeger
M
M
Thörner
B
B
Diener
B
B
Hermann
M
M
D000279:Administration, Cutaneous; D000293:Adolescent; D000328:Adult; D002518:Ceramides / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis / Q000096:biosynthesis; D004655:Emulsions / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis; D007509:Irritants / Q000009:adverse effects; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009824:Ointments / Q000008:administration & dosage*; D010577:Petrolatum / Q000008:administration & dosage*; D020011:Protective Agents / Q000008:administration & dosage*; D015203:Reproducibility of Results; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012869:Skin Absorption / Q000187:drug effects*; D023422:Skin Irritancy Tests / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects
D002518:Ceramides; D004655:Emulsions; D007375:Interleukin-1; D007509:Irritants; D009824:Ointments; D020011:Protective Agents; D012967:Sodium Dodecyl Sulfate; D010577:Petrolatum; D007770:L-Lactate Dehydrogenase; D015232:Dinoprostone