Proliferation and differentiation of neural stem cells in adult rats after cerebral infarction.
OBJECTIVE
To investigate proliferation and differentiation of neural stem cells in adult rats after cerebral infarction.


METHODS
Models of cerebral infarction in rats were made and the time-course expression of bromodeoxyuridine (BrdU), Musashi1, glial fibrillary acidic protein (GFAP), and neuronal nuclear antigen (NeuN) were determined by immunohistochemistry and immunofluorescence staining. BrdU and Musashi1 were used to mark dividing neural stem cells. GFAP and NeuN were used to mark differentiating neural stem cells.


RESULTS
Compared with controls, the number of BrdU-labeled and BrdU-labeled with Musashi 1-positive cells increased strikingly 1 day after cerebral infarction; approximately 6 fold with a peak 7 days later; markedly decreased 14 days later, but was still elevated compared with that of controls; decling to the control level 28 days later. The number of BrdU-labeled with GFAP-positive cells nearly remained unchanged in the hippocampus after cerebral infarction. The number of BrdU-labeled with NeuN-positive cells increased strikingly 14 days after cerebral infarction, reached maximum peak in the hippocampus 28 days after cerebral infarction in rats.


CONCLUSIONS
Cerebral infarction stimulate proliferation of inherent neural stem cells and most proliferated neural stem cells differentiate into neurons.
Zhang
Bo
B
Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730.
Wang
Ren-Zhi
RZ
Yao
Yong
Y
Liu
Zhi-Hai
ZH
Lian
Zhi-Gang
ZG
Zou
Yu-Jie
YJ
Wei
Yu-Kui
YK
D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*
D034961:Antigens, Nuclear; D005904:Glial Fibrillary Acidic Protein; C491296:Msi1 protein, rat; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D001973:Bromodeoxyuridine