title: High level activity of 2', 5'-oligoadenylate synthetase in dog serum.
abstract: Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 +/- 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 +/- 2.1), rabbit (4.0 +/- 1.1), and guinea pig (0.3 +/- 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iwata
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Miyuki
authors.initials: M
authors.lastname: Fujino
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sato
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hosokawa-Kanai
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tuchiya
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sokawa
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000097:blood*; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western / Q000662:veterinary; D002415:Cats; D002848:Chromatography, DEAE-Cellulose / Q000662:veterinary; D004285:Dogs / Q000097:blood*; D005260:Female; D006168:Guinea Pigs; D011817:Rabbits; D011863:Radioimmunoassay / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D015088:2',5'-Oligoadenylate Synthetase