Acute effects of Microcystis aeruginosa from the Patos Lagoon estuary, southern Brazil, on the microcrustacean kalliapseudes schubartii (Crustacea: Tanaidacea).
Toxic blooms of the cyanobacterium Microcystis aeruginosa, a microcystin producer, have been observed in the past two decades in the Patos Lagoon estuary (southern Brazil). This cyanobacterium reaches the estuary from northern waters and accumulates as toxic blooms in the shallow margins of the environment. Microcystins are phosphatase (PP1 and PP2A) inhibitors and cause animal death via alteration of the liver cell cytoskeletons and intrahepatic hemorrhage. The massive accumulation of toxic material affects the survival of several benthonic estuarine local organisms. The tanaidacea Kalliapseudes schubartii is a benthonic estuarine species which occurs at high densities throughout the year in mixohaline areas of the Patos Lagoon. This microcrustacean is of high ecological relevance and plays an important role in the estuarine food web, as it is consumed on a large scale by estuarine fish. This work verifies the acute toxicity of aqueous extracts of M. aeruginosa RST9501 and of sediments spiked with lyophilized material of the same strain on K. schubartii; it also evaluates the sublethal effects on tanaidacean oxygen consumption rates and glycogen levels under acute exposure to M. aeruginosa aqueous extracts. The strain M. aeruginosa RST9501 was cultured in BGN/2 medium. The aqueous extracts were prepared using the lyophilized material from the strain cultures. Acute tests were performed over 96 h at a salinity of 15, at six toxic concentrations, and resulted in an average 96-h LC50 of 1.44 mg ml(-1). The spiked sediment tests were performed with a 10-day duration, using the lyophilized material in three proportions of powder/sediment and showed an average LC50 of 1.79 mg ml(-1). Oxygen consumption was determined after 24 and 48 h of incubation in adult organisms exposed to sublethal aqueous extract concentrations and showed a significant increase at the highest concentrations. This suggests alterations in the organism's metabolism by exposure to the cyanobacterium extract. The glycogen levels were determined with a commercial kit (Glicox 500; DOLES Ltd.); after 24 and 48 h the dosages were administered in the same organisms utilized in the oxygen consumption test and did not demonstrate significant differences. The results demonstrate the possible risks of intoxication to which the natural populations of K. schubartii were exposed in the environment and emphasize the importance of studies involving sublethal concentrations of M. aeruginosa to other organisms of the trophic web in this aquatic system.
Montagnolli
W
W
Departamento de Quimica, Fundação Universidade Federal do Rio Grande, Brazil.
Zamboni
A
A
Luvizotto-Santos
R
R
Yunes
J S
JS
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D001938:Brazil; D003445:Crustacea / Q000187:drug effects*; D019015:Geologic Sediments / Q000737:chemistry; D006003:Glycogen / Q000378:metabolism; D007928:Lethal Dose 50; D052998:Microcystins; D046931:Microcystis / Q000737:chemistry*; D009792:Oceans and Seas; D010100:Oxygen / Q000378:metabolism; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000633:toxicity*; D023382:Toxicity Tests, Acute
D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D006003:Glycogen; D010100:Oxygen