title: In vitro activity and killing effect of the synthetic hybrid cecropin A-melittin peptide CA(1-7)M(2-9)NH(2) on methicillin-resistant nosocomial isolates of Staphylococcus aureus and interactions with clinically used antibiotics.
abstract: The in vitro activity of CA(1-7)M(2-9)NH(2), a 15-residue synthetic hybrid peptide derived from the sequences of cecropin A and melittin, alone and in combination with amoxicillin-clavulanate, imipenem, clarithromycin, ciprofloxacin, rifampin, and vancomycin, was investigated against 40 nosocomial isolates of methicillin-resistant Staphylococcus aureus. Antimicrobial activity of CA(1-7)M(2-9)NH(2) was measured by minimal inhibitory concentration, MBC, and time-kill studies. All isolates were inhibited at concentrations of 1 to 16 microg/mL. Combination studies performed with S. aureusATCC 43300 demonstrated synergy only when CA(1-7)M(2-9)NH(2) was combined with amoxicillin-clavulanate and imipenem. Our findings show that CA(1-7)M(2-9)NH(2) is active against methicillin-resistant S. aureusand that its activity is enhanced when it is combined with several antimicrobial agents.
authors.affiliation: Institute of Infectious Diseases and Public Health, University of Ancona, Ancona, Italy. anonacmi@interfree.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Giacometti
authors.forename: Oscar
authors.initials: O
authors.lastname: Cirioni
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kamysz
authors.forename: Giuseppina
authors.initials: G
authors.lastname: D'Amato
authors.forename: Carmela
authors.initials: C
authors.lastname: Silvestri
authors.forename: Maria
authors.initials: M
authors.lastname: Simona Del Prete
authors.forename: Jerzy
authors.initials: J
authors.lastname: Lukasiak
authors.forename: Giorgio
authors.initials: G
authors.lastname: Scalise
mesh_terms: D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology*; D003428:Cross Infection / Q000188:drug therapy; D004347:Drug Interactions; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008297:Male; D008555:Melitten / Q000494:pharmacology*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D008555:Melitten; D015378:Imipenem; D019980:Amoxicillin-Potassium Clavulanate Combination