Separation and conductimetric detection of C1-C7 aliphatic monocarboxylic acids and C1-C7 aliphatic monoamines on unfunctionized polymethacrylate resin columns.
The application of unfunctionized polymethacrylate resin (TSKgel G3000PWXL) as a stationary phase in liquid chromatography with conductimetric detection for C1-C7 aliphatic monocarboxylic acids (formic acid, acetic acid, propionic acid, butyric acid, isovaleric acid, valeric acid, 3,3-dimethylbutyric acid, 4-methylvaleric acid, hexanoic acid, 2-methylhexanoic acid, 5-methylhexanoic acid and heptanoic acid) and C1-C7 aliphatic monoamines (methylamine, ethylamine, propylamine, isobutylamine, butylamine, isoamylamine, amylamine, 1,3-dimethylbutylamine, hexylamine, 2-heptylamine and heptylamine) was attempted with C8 aliphatic monocarboxylic acids (2-propylvaleric acid, 2-ethylhexanoic acid, 2-methylheptanoic acid and octanoic acid) and C8 aliphatic monoamines (1,5-dimethylhexylamine, 2-ethylhexylamine, 1-methylheptylamine and octylamine) as eluents, respectively. Using 1 mM 2-methylheptanoic acid at pH 4.0 as the eluent, excellent separation and relatively high sensitive detection for these C1-C7 carboxylic acids were achieved on a TSKgel G3000PWXL column (150 mm x 6 mm i.d.) in 60 min. Using 2 mM octylamine at pH 11.0 as the eluent, excellent separation and relatively high sensitive detection for these C1-C7 amines were also achieved on the TSKgel G3000PWXL column in 60 min.
Ohta
Kazutoku
K
Ceramics Research Institute, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora, Simoshidami, Moriyama-ku, Nagoya 463-8560, Japan. kazu.ohta@aist.go.jp
Towata
Atsuya
A
Ohashi
Masayoshi
M
Takeuchi
Toyohide
T
D000588:Amines / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000588:Amines; D002264:Carboxylic Acids; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid