Clinical application of marrow mesenchymal stem cells for hard tissue repair.
Human marrow mesenchymal stem cells were cultured in a medium containing glycerophosphate, ascorbic acid, and dexamethasone (Dex) on alumina ceramic discs and on tissue culture polystyrene (TCPS) dishes. Cell proliferation followed by osteogenic differentiation was observed to be equal on both culture substrata. The differentiation resulted in the appearance of bone-forming osteoblasts, which fabricated mineralized matrices on these substrata. Stem cells kept at 4 degrees C for 24 h outside a CO2 incubator maintained a viability level of more than 90%. The regenerative cultured bone outside the incubator also maintained high alkaline phosphatase activity for several hours. These results verified that cultured bone fabricated at a cell processing center can be transported to distant hospitals for use in hard tissue repair. To date, the tissue engineered cultured bone formed on alumina ceramics in this environment have already been used in clinical situations, such as total ceramic ankle replacements.
Ohgushi
Hajime
H
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Amagasaki, Hyogo, Japan. hajime-ohgushi@aist.go.jp
Kitamura
Shigeyuki
S
Kotobuki
Noriko
N
Hirose
Motohiro
M
Machida
Hiroko
H
Muraki
Kaori
K
Takakura
Yoshinori
Y
D000328:Adult; D000368:Aged; D000537:Aluminum Oxide; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002455:Cell Division; D002516:Ceramics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010012:Osteogenesis; D023822:Tissue Engineering
D000537:Aluminum Oxide