title: ATR-FTIR spectroscopy studies of iron-sulfur protein and cytochrome c1 in the Rhodobacter capsulatus cytochrome bc1 complex.
abstract: Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the midpoint potential of heme was lowered from the wild-type value of 320 mV to 60 mV. Overall redox difference spectra of the wild type and M183K mutant were essentially identical, indicating that the mutation did not cause any major structural perturbation. Spectra were compared with data on the bovine bc(1) complex, and tentative assignments of several bands could be made by comparison with available data on model compounds and crystallographic structures. The bacterial spectra showed contributions from ubiquinone that were much larger than in the bovine enzyme, arising from additional bound and adventitious ubiquinone. The M183K mutant enabled selective reduction of the iron-sulfur protein which in turn allowed the IR redox difference spectra of ISP and cytochrome c(1) to be deconvoluted at high signal/noise ratios, and features of these spectra are interpreted in light of structural and mechanistic information.
authors.affiliation: Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Masayo
authors.initials: M
authors.lastname: Iwaki
authors.forename: Artur
authors.initials: A
authors.lastname: Osyczka
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Moser
authors.forename: P Leslie
authors.initials: PL
authors.lastname: Dutton
authors.forename: Peter R
authors.initials: PR
authors.lastname: Rich
mesh_terms: D003575:Cytochromes c1 / Q000737:chemistry*; D014450:Electron Transport Complex III / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D008958:Models, Molecular; D016354:Rhodobacter capsulatus / Q000201:enzymology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D007506:Iron-Sulfur Proteins; D003575:Cytochromes c1; D014450:Electron Transport Complex III