Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA.
We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.
Buller
Richard S
RS
Department of Pediatrics, Box 8116, Washington University School of Medicine at St. Louis Children's Hospital, One Children's Place, St. Louis, MO 63110, USA. buller@kids.wustl.edu
Storch
Gregory
G
D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004279:DNA, Viral