title: Cellular-free magnesium depletion in brain and muscle of normal and preeclamptic pregnancy: a nuclear magnetic resonance spectroscopic study.
abstract: Preeclampsia is a pregnancy disorder of unknown origin, characterized by vasospasm, elevated blood pressure, and increased neuromuscular irritability, features common to syndromes of magnesium deficiency. Evidence of serum and ionized magnesium metabolism disturbances have been observed in women with preeclampsia. This and the therapeutic utility of magnesium in preeclampsia led us to investigate the extent to which an endogenous tissue magnesium deficiency might be present in and contribute to its pathophysiology. We used (31)P nuclear magnetic resonance spectroscopy to noninvasively measure in situ intracellular-free magnesium levels in brain and skeletal muscle of fasting nonpregnant women (n=12), and of third trimester women with uncomplicated pregnancies (n=11) and preeclampsia (n=7). Compared with nonpregnant controls (brain 519+/-59 micromol/L; muscle 604+/-34 micromol/L), brain and skeletal muscle intracellular magnesium levels were significantly lower in both normal pregnant (brain 342+/-23 micromol/L; muscle 482+/-40 micromol/L; P=0.05 for both tissues) and preeclamptic women (brain 229+/-17 micromol/L; muscle 433+/-46 micromol/L; P=0.05 for both tissues). Brain intracellular magnesium was further reduced in preeclamptics compared with normal pregnant subjects (P=0.05). For all pregnant subjects, blood pressure was significantly and inversely related to the concomitantly measured intracellular magnesium level in brain (systolic, r=-0.59, P=0.01; diastolic, r=-0.52, P=0.02) but not in muscle. Cellular magnesium depletion is characteristic of normal pregnancy and may be one factor contributing to the pathophysiology of preeclampsia. Furthermore, the influence of central nervous system factors on blood pressure may be mediated, at least in part, by ambient intracellular magnesium levels.
authors.affiliation: Weill Medical College of Cornell University, New York, NY, USA.
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Resnick
authors.forename: Mario
authors.initials: M
authors.lastname: Barbagallo
authors.forename: Mordechai
authors.initials: M
authors.lastname: Bardicef
authors.forename: Orit
authors.initials: O
authors.lastname: Bardicef
authors.forename: Yoram
authors.initials: Y
authors.lastname: Sorokin
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Evelhoch
authors.forename: Ligia J
authors.initials: LJ
authors.lastname: Dominguez
authors.forename: Brian A
authors.initials: BA
authors.lastname: Mason
authors.forename: David B
authors.initials: DB
authors.lastname: Cotton
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D001794:Blood Pressure; D001923:Brain Chemistry; D005215:Fasting; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000737:chemistry; D008274:Magnesium / Q000032:analysis; D008275:Magnesium Deficiency / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000503:physiopathology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
chemical_list: D000255:Adenosine Triphosphate; D008274:Magnesium