Phenotypic plasticity in adult worms of Schistosoma mansoni (Trematoda:Schistosomatidae) evidenced by brightfield and confocal laser scanning microscopies.
A comparative morphometric study was performed to identify host-induced morphological alterations in Schistosoma mansoni adult worms. A wild parasite population was obtained from a naturally infected rodent (Nectomys squamipes) and then recovered from laboratory infected C3H/He mice. Furthermore, allopatric worm populations maintained for long-term under laboratory conditions in Swiss Webster mice were passed on to N. squamipes. Suckers and genital system (testicular lobes, uterine egg, and egg spine) were analyzed by a digital system for image analysis. Confocal laser scanning microscopy (CLSM) showed details of the genital system (testicular lobes, vitelline glands, and ovary) and the tegument just below the ventral sucker. Significant morphological changes (p < 0.05) were detected in male worms in all experimental conditions, with no significant variability as assessed by CLSM. Significant changes (p < 0.05) were evident in females from the wild population related to their ovaries and vitelline glands, whereas allopatric females presented differences only in this last character. We conclude that S. mansoni worms present the phenotypic plasticity induced by modifications in the parasite's microenvironment, mainly during the first passage under laboratory conditions.
Neves
Renata Heisler
RH
Disciplina de Parasitologia, Departamento de Patologia e Laboratórios, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rua Prof. Manoel de Abreu 444, 5 andar, 20551-170 Rio de Janeiro, RJ, Brasila.
Costa-Silva
Michele
M
Martinez
Elaine Machado
EM
Branquinho
Thiago B
TB
de Oliveira
Regina Maria Figueiredo
RM
Lenzi
Henrique Leonel
HL
Gomes
Delir Corrêa
DC
Machado-Silva
José Roberto
JR
D000818:Animals; D005260:Female; D006790:Host-Parasite Interactions; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H / Q000469:parasitology; D018613:Microscopy, Confocal; D010053:Ovary / Q000648:ultrastructure; D010641:Phenotype; D012377:Rodentia / Q000469:parasitology; D012550:Schistosoma mansoni / Q000648:ultrastructure*; D013737:Testis / Q000648:ultrastructure