Antigen-specific lymphocyte sequestration in lymphoid organs: lack of essential roles for alphaL and alpha4 integrin-dependent adhesion or Galphai protein-coupled receptor signaling.
Selective lymphocyte sequestration was described over 30 years ago as the transient withdrawal of Ag-specific lymphocytes from the circulation as a result of their activation in secondary lymphoid organs. We used a TCR-transgenic adoptive transfer system to further characterize the Ag and adjuvant dependence of this process in mice. In addition, we examined the contribution of the alpha(L) and alpha(4) integrin chains as well as Galpha(i) protein-coupled receptor signaling to the retention of Ag-specific T cells in peripheral lymph nodes. Our results demonstrate that selective lymphocyte sequestration is T cell autonomous and adjuvant independent, and that the duration of sequestration is not controlled by the continued presence of Ag in secondary lymphoid organs. This process is not critically dependent on the alpha(L) and alpha(4) integrin chains or Galpha(i) protein-coupled receptor signaling. Selective lymphocyte sequestration may be mediated by redundant mechanisms and/or controlled by novel or nonclassical adhesion or trafficking molecules.
Arnold
Carrie N
CN
Department of Pathology, Laboratory of Immunology and Vascular Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. aggie99@stanford.edu
Butcher
Eugene C
EC
Campbell
Daniel J
DJ
D019264:Adoptive Transfer; D000818:Animals; D000941:Antigens / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D016023:Integrins / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D008221:Lymphoid Tissue / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D051379:Mice; D037342:Pertussis Toxin / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000502:physiology
D000941:Antigens; D016023:Integrins; D043562:Receptors, G-Protein-Coupled; D037342:Pertussis Toxin