Unraveling genes and pathways influenced by H-prune PDE overexpression: a model to study cellular motility.
H-prune, a new cyclic nucleotide phosphodiesterase, binds to nm23-H1, a metastasis suppressor protein. The overexpression of h-prune in the MDA-MB-435 breast carcinoma cell line causes a substantial decrease of cAMP, and an increase in cellular motility. This latest effect is correlated both to the h-prune phosphodiesterase activity and to the interaction between h-prune and nm23-H1 proteins. Understanding the molecular changes in tumor cells with an increased level of expression of h-prune might shed light on motility processes, which are the driving forces of the cells to move away from the primary tumor and to become metastatic. This report overview genes and pathways influenced by h-prune overexpression in a conventional breast cancer cellular model.
D'Angelo
Anna
A
Telethon Institute of Genetics and Medicine, Naples, Italy.
Zollo
Massimo
M
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000556:secondary; D002352:Carrier Proteins / Q000096:biosynthesis*; D002465:Cell Movement / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008957:Models, Genetic; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases
D002352:Carrier Proteins; C438732:PRUNE1 protein, human; D010744:Phosphoric Monoester Hydrolases; D010727:Phosphoric Diester Hydrolases