In vitro monitoring of nanogram levels of puerarin in human urine using flow injection chemiluminescence.
A rapid and sensitive chemiluminescence (CL) determination of puerarin with the flow injection technique was presented. It was found that puerarin could enhance the CL generated from luminol-KIO(4) system significantly. The increment of CL intensity was linear over the concentration of puerarin ranging from 0.3 to 100.0 ng mL(-1) (r(2)=0.9963), offering a detection limit as low as 0.1 ng mL(-1) (3 sigma). At a flow rate of 2.0 mL min(-1), one analysis cycle, including sampling and washing, could be accomplished in 20 s with a relative standard deviation of less than 5.0%. The experimental results demonstrated that the excretive amounts of puerarin reached its maximum in 3 h after taking 250 mL green drink containing 275 mg puerarin, and the puerarin excretive ratio during 24 h was 9.70% in the body of volunteers.
Wang
Changna
C
Department of Chemistry, Northwest University, Taibai Road 229, Xi'an 710069, China.
Song
Zhenghua
Z
D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D007529:Isoflavones / Q000652:urine*; D049449:Luminescence; D036103:Nanotechnology; D012680:Sensitivity and Specificity
D007529:Isoflavones; C033607:puerarin