Rack-1, GAPDH3, and actin: proteins of Myzus persicae potentially involved in the transcytosis of beet western yellows virus particles in the aphid.
Beet western yellows virus (BWYV) is a Polerovirus that relies on the aphid Myzus persicae for its transmission, in a persistent-circulative mode. To be transmitted, the virus must cross the midgut and the accessory salivary glands (ASG) epithelial barriers in a transcytosis mechanism where vector receptors interact with virions. In this paper, we report in vitro interaction experiments between BWYV and aphid components. Using the M. persicae clone from Colmar, we showed that a set of aphid polypeptides, separated by SDS-PAGE or 2D electrophoresis (2DE), can bind in vitro to purified wild type or mutant particles. Using subcellular fractionation, we showed that the 65-kDa polypeptide identified as symbionin is a soluble protein whereas the other polypeptides seem to be associated more or less strongly to the membrane. We hypothesize that three polypeptides, identified by mass spectrometry as Rack-1, GAPDH3, and actin, may be involved in the epithelial transcytosis of virus particles in the aphid vector.
Seddas
Pascale
P
Unité de Recherche Biologie des Interactions Virus/vecteurs, Institut National de la Recherche Agronomique, 68021 Colmar cedex, France. pseddas@colmar.inra.fr
Boissinot
Sylvaine
S
Strub
Jean-Marc
JM
Van Dorsselaer
Alain
A
Van Regenmortel
Marc H V
MH
Pattus
Franc
F
D000199:Actins / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000378:metabolism* / Q000821:virology*; D027461:Beta vulgaris / Q000821:virology; D001693:Biological Transport, Active; D015180:Electrophoresis, Gel, Two-Dimensional; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D019476:Insect Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007303:Insect Vectors / Q000378:metabolism* / Q000821:virology*; D017793:Luteovirus / Q000235:genetics / Q000472:pathogenicity*; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D011485:Protein Binding; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D000199:Actins; D019476:Insect Proteins; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases