Estimating the energetic contribution of hydrogen bonding to the stability of Candida methylica formate dehydrogenase by using double mutant cycle.
An homology model of Candida methylica formate dehydrogenase (cm FDH) was constructed based on the Pseudomonas sp. 101 formate dehydrogenase (ps FDH) structure. In wild type cm FDH, Thr169 and Thr226 can form hydrogen bonds with each other. We measured the interaction energy between the two threonines independent of other interactions in the proteins by using a so-called double mutant cycle and assessing the protein stability from the concentration of guanidine hydrochloride needed to denature 50% of the molecules. We conclude that the hydrogen bonds stabilize the wild type protein by -4 kcal mol(-1).
Karagüler
Nevin Gül
NG
Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK. karaguler@itu.edu.tr
Sessions
Richard B
RB
Moreton
Kathleen M
KM
Clarke
Anthony R
AR
Holbrook
J John
JJ
D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000737:chemistry*; D019791:Guanidine / Q000737:chemistry; D006860:Hydrogen Bonding; D007700:Kinetics; D008962:Models, Theoretical; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011549:Pseudomonas / Q000378:metabolism; D012984:Software; D013816:Thermodynamics; D013912:Threonine / Q000378:metabolism
D013912:Threonine; D005560:Formate Dehydrogenases; D019791:Guanidine