The complete structure of the lipooligosaccharide from the halophilic bacterium Pseudoalteromonas issachenkonii KMM 3549T.
Novel lipooligosaccharide components were isolated and identified from the lipooligosaccharide fraction of the halophilic marine bacterium Pseudoalteromonas issachenkonii type strain KMM 3549T. The complete structure was achieved by chemical analysis, 2D NMR spectroscopy and MALDI mass spectrometry as the following: [carbohydrate formula see text] All sugars are d-pyranoses. Hep is L-glycero-D-manno-heptose, Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid, P is phosphate, residues and substituents in italic are not stoichiometrically linked. In addition, by MALDI mass spectrometry of the intact LOS, the lipid A moiety was also identified as a mixture of penta-, tetra- and triacylated species.
Silipo
Alba
A
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy.
Leone
Serena
S
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Sturiale
Luisa
L
Garozzo
Domenico
D
Nazarenko
Evgeny L
EL
Gorshkova
Raisa P
RP
Ivanova
Elena P
EP
Gorshkova
Natalya M
NM
Molinaro
Antonio
A
D001406:Bacillaceae / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D031161:Pseudoalteromonas / Q000737:chemistry* / Q000145:classification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013379:Substrate Specificity
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides