In vitro autoradiographic localization of calcitonin and amylin binding sites in monkey brain.
Calcitonin (CT) and amylin are related peptides with potent central actions, including suppression of appetite and gastric acid secretion. Little is known about the distribution and binding characteristics of amylin receptors in species other than rat; therefore, in this study, by using in vitro autoradiography, we have mapped the distribution of 125I-rat amylin binding sites in the monkey brain and compared this distribution to that of binding sites for 125I-salmon CT (125I-sCT). Highest densities of 125I-amylin binding were in the hypothalamus, including the arcuate nucleus and parts of the ventromedial hypothalamic nuclei, and the solitary nucleus. Rostrally, moderate to high density binding was present in parts of the preoptic area, bed nucleus of the stria terminalis, amygdala and accumbens nucleus (Acb). Caudally, binding of amylin was more restricted, with moderate to high density binding present only in dorsal raphe, and area postrema. The primary visual cortex displayed strong and periodic CT binding in layer 4. The subcortical pattern of distribution of amylin and CT receptors in the monkey was similar to that seen previously in the rat, although the relative densities of binding to different brain structures were not always conserved. As with rat, monkey amylin receptors were a subset of the sites labeled with 125I-sCT. Analysis of receptor specificity indicated a greater relative potency of CT peptides in competing for 125I-amylin binding in monkey, when compared to rat, while, there was a decrease in the relative potency of CT gene-related peptides, potentially due to differences the level of receptor activity modifying proteins (RAMPs) in monkey versus rat brain. Amylin receptors in primates are likely to perform a similar role to those in rats; however, the interaction of the receptors with related peptides may differ.
Paxinos
G
G
Prince of Wales Medical Research Institute, The University of New South Wales, Sydney 2052, Australia. g.paxinos@unsw.edu.au
Chai
S Y
SY
Christopoulos
G
G
Huang
X-F
XF
Toga
A W
AW
Wang
H Q
HQ
Sexton
P M
PM
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites / Q000502:physiology; D001667:Binding, Competitive / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001931:Brain Mapping; D002116:Calcitonin / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D058228:Islet Amyloid Polypeptide; D008032:Limbic System / Q000166:cytology / Q000378:metabolism; D008251:Macaca; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D018003:Receptors, Calcitonin / Q000378:metabolism*; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide / Q000378:metabolism*; D013045:Species Specificity; D044767:Ubiquitin-Protein Ligases
D000682:Amyloid; C429530:DTL protein, human; D007457:Iodine Radioisotopes; D058228:Islet Amyloid Polypeptide; D009687:Nuclear Proteins; D018003:Receptors, Calcitonin; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide; D002116:Calcitonin; D044767:Ubiquitin-Protein Ligases