title: From the Cover: The dynamics of genomic-length DNA molecules in 100-nm channels.
abstract: We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min.
authors.affiliation: Department of Physics, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Jonas O
authors.initials: JO
authors.lastname: Tegenfeldt
authors.forename: Christelle
authors.initials: C
authors.lastname: Prinz
authors.forename: Han
authors.initials: H
authors.lastname: Cao
authors.forename: Steven
authors.initials: S
authors.lastname: Chou
authors.forename: Walter W
authors.initials: WW
authors.lastname: Reisner
authors.forename: Robert
authors.initials: R
authors.lastname: Riehn
authors.forename: Yan Mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Edward C
authors.initials: EC
authors.lastname: Cox
authors.forename: James C
authors.initials: JC
authors.lastname: Sturm
authors.forename: Pascal
authors.initials: P
authors.lastname: Silberzan
authors.forename: Robert H
authors.initials: RH
authors.lastname: Austin
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D016678:Genome; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA