NF-kappaB prevents TNF-alpha-induced apoptosis in an oligodendrocyte cell line.
Nuclear factor kappa beta (NF-kappaB) inhibits apoptosis in sensory, hippocampal, and striatal neurons of the central nervous system. Although several apoptotic stimuli have been shown to activate NF-kappaB in oligodendrocytes, the function of NF-kappaB in this cell type remains unknown. In this study, we introduced plasmids expressing either the p50- or p65-subunit of human NF-kappaB into Central Glia-4 (CG-4)--a rat oligodendrocyte precursor cell line-and determined the influence of NF-kappaB function on tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis. Expression of NF-kappaB markedly prevented CG-4 apoptosis, with p50 being more effective than p65. This anti-apoptotic activity was repressed by IkappaB-alpha, an inhibitor of NF-kappaB. These results imply that NF-kappaB acts as a potent inhibitor of TNF-induced apoptosis in oligodendrocytes.
Hamanoue
Makoto
M
Department of Physiology, Toho University, School of Medicine, 5-21-16 Ohmori-nishi, Ohta-ku, Tokyo 143-8540, Japan. hamanoue-ns@umin.ac.jp
Yoshioka
Akira
A
Ohashi
Toya
T
Eto
Yoshikatsu
Y
Takamatsu
Ken
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha