Use of fluorescent lectin probes for analysis of footprints from Pseudomonas aeruginosa MDC on hydrophilic and hydrophobic glass substrata.
Microbial footprints of Pseudomonas aeruginosa MDC attached for 1 h to clean or silanized glass were analyzed with fluorescently labeled lectin probes. Footprint composition varied, depending on cell physiology and substratum surface chemistry. This suggests that substratum physicochemistry affected the structure of cell surfaces of adsorbed organisms.
Bejarano
Eduardo Mora
EM
Departament of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, CEP 05508-900, São Paulo, São Paulo, Brazil.
Schneider
René Peter
RP
D001422:Bacterial Adhesion; D005456:Fluorescent Dyes; D005898:Glass; D037102:Lectins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*
D005456:Fluorescent Dyes; D037102:Lectins; D008070:Lipopolysaccharides