title: The use of FTIR microscopy for evaluation of herpes viruses infection development kinetics.
abstract: The kinetics of Herpes simplex infection development was studied using an FTIR microscopy (FTIR-M) method. The family of herpes viruses includes several members like H. simplex types I and II (HSV I, II), Varicella zoster (VZV) viruses which are involved in various human and animal infections of different parts of the body. In our previous study, we found significant spectral differences between normal uninfected cells in cultures and cells infected with herpes viruses at early stages of the infection. In the present study, cells in cultures were infected with either HSV-I or VZV and at various times post-infection they were examined either by optical microscopy or by advanced FTIR-M. Spectroscopic measurements show a consistent decrease in the intensity of the carbohydrate peak in correlation with the viral infection development, observed by optical microscopy. This decrease in cellular carbohydrate level was used as indicator for herpes viruses infection kinetics. This parameter could be used as a basis for applying a spectroscopic method for the evaluation of herpes virus infection development. Our results show also that the development kinetics of viral infection has an exponential character for these viruses.
authors.affiliation: The Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. evitaly@bgumail.bgu.ac.il
authors.forename: Vitaly
authors.initials: V
authors.lastname: Erukhimovitch
authors.forename: Igor
authors.initials: I
authors.lastname: Mukmanov
authors.forename: Marina
authors.initials: M
authors.lastname: Talyshinsky
authors.forename: Yelena
authors.initials: Y
authors.lastname: Souprun
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Huleihel
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates; D002623:Chemistry Techniques, Analytical; D002522:Chlorocebus aethiops; D006564:Herpesviridae / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014709:Vero Cells
chemical_list: D002241:Carbohydrates