Amplification of MGC2177, PLAG1, PSMC6P, and LYN in a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification.
Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10-20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications (MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2 approximately q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.
Tsang
Yvonne T M
YT
Texas Children's Cancer Center, Cancer Genomics Group, MC3-3320, Department of Pediatrics, Baylor College of Medicine, 6621 Fannin Street, Houston, TX 77030, USA.
Chang
Yi-Mieng
YM
Lu
Xinyan
X
Rao
Pulivarthi H
PH
Lau
Ching C
CC
Wong
Kwong-Kwok
KK
D000251:Adenosine Triphosphatases / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005784:Gene Amplification; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018198:Mixed Tumor, Malignant / Q000235:genetics* / Q000473:pathology; D009097:Multienzyme Complexes / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D046988:Proteasome Endopeptidase Complex; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D019061:src-Family Kinases / Q000235:genetics*
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009097:Multienzyme Complexes; C104382:PLAG1 protein, human; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases