Proteomics: technologies and applications.
Enabling technologies for proteomics (studies examining the protein complement of the genome) have been in development for over 20 years. However, more recently, the field has become formalized by combining the techniques for large-scale protein separation (two-dimensional electrophoresis) with very precise, high fidelity approaches to the analysis and characterisation of the separated proteins (mass spectrometry). These approaches bring to reality the powerful scope of proteomics, enabling the interpretation of cell physiology through profiling the molecular biodescriptors of gene expression, the proteins. Applications of proteomics are wide ranging and can be found within many disciplines, although one of the more developed fields that is examined here covers the arena of toxicological and pharmacological profiling. In this paper, the current technologies that make proteomics possible will be looked at. Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development.
Molloy
Mark P
MP
Pfizer Global Research and Development, Ann Arbor Laboratories, USA.
Witzmann
Frank A
FA
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics; D012995:Solubility
D011506:Proteins