title: Validation of method for total selenium determination in yeast by flame atomic absorption spectrometry.
abstract: A procedure using open digestion followed by flame atomic absorption spectrometry is described for measuring the total selenium content of Se-enriched yeast. The limits of detection and quantitation were 2.5 mg/L and 5 mg/L Se, respectively. The signal response was linear over the range of 5-50 mg/L Se, and the average recovery from spiked samples was 98.9%. The validated method was used to measure the Se content of Se-enriched yeast reference material and produced a result of 2145 +/- 38 mg/kg (n = 3), which is in good agreement with the certified level of 2125 +/- 65 mg/kg.
authors.affiliation: Alltech Biotechnology Centre, Dunboyne, Co. Meath, Ireland.
authors.forename: Cathal D
authors.initials: CD
authors.lastname: Connolly
authors.forename: Ronan F
authors.initials: RF
authors.lastname: Power
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hynes
mesh_terms: D002138:Calibration; D012015:Reference Standards; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis* / Q000592:standards; D013054:Spectrophotometry, Atomic / Q000379:methods / Q000592:standards; D015003:Yeasts / Q000737:chemistry*
chemical_list: D012643:Selenium