title: [C-reactive protein inhibits the expression of hypoxia-inducible factor-1alpha in human umbilical vein endothelial cells in hypoxic condition].
abstract: OBJECTIVE
  To investigate the effect of C-reactive protein (CRP) on expression of hypoxia-inducible factor-1alpha (HIF-1alpha).
  
  
  METHODS
  Using CoCl(2) (100 micromol/L) to simulate hypoxic condition for culturing human umbilical vein endothelial cells (HUVECs), we examined the effects of CRP on expression of HIF-1alpha. The proteins were extracted from the cells after a 24-hour exposure of the cells to CRP of varied concentrations in the presence of CoCl(2), and Western blotting was performed for quantification of HIF-1alpha expression, the results were analyzed statistically with SPSS software.
  
  
  RESULTS
  CRP at the concentration of 5 microg/ml decreased the expression of HIF-1alpha (P<0.001), producing the maximum inhibitory effect at the concentration of 100 microg/ml, an effect exhibiting dose-dependence.
  
  
  CONCLUSIONS
  CRP inhibits the expression of HIF-1alpha in HUVECs subjected to hypoxic condition, which serves as an important evidence for the inhibitory effect of CRP on angiogenesis in hypoxic condition.
authors.affiliation: Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yfsong789@yahoo.com.cn
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Song
authors.forename: Ping-sheng
authors.initials: PS
authors.lastname: Wu
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Lai
mesh_terms: D015153:Blotting, Western; D002097:C-Reactive Protein / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000032:analysis*; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D004268:DNA-Binding Proteins; C497442:HIF1A protein, human; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors; D003035:Cobalt; D002097:C-Reactive Protein; C018021:cobaltous chloride