CpG-oligodeoxynucleotides enhance porcine immunity to Toxoplasma gondii.
Protection against a challenge infection with Toxoplasma gondii VEG strain oocysts was examined in pigs after vaccination with T. gondii RH strain tachyzoites with or without a porcine specific synthetic oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs. Six groups of pigs were immunized with incomplete Freund's adjuvant (IFA) and either vehicle, tachyzoites alone or in combination with three different doses of CpG ODN or with CpG ODN alone. Protection from challenge was significantly (P < 0.05) improved in pigs vaccinated using CpG ODN as an adjuvant with tachyzoites compared to all other groups. The CpG ODN tachyzoite-immunized pigs also had higher serum parasite specific IgG antibody, no clinical signs of disease, and 52% had no demonstrable tissue cysts after the challenge infection. These data indicate that CpG ODN is a potential safe and effective adjuvant for the T. gondii RH strain vaccine in pigs.
Kringel
H
H
Danish Center for Experimental Parasitology, The Royal Veterinary and Agricultural University, Dyrlaegevej 100, DK-1870 Frederiksberg C, Denmark.
Dubey
J P
JP
Beshah
E
E
Hecker
R
R
Urban
J F
JF
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001831:Body Temperature / Q000276:immunology; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D014611:Vaccination / Q000379:methods / Q000662:veterinary*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides