Effects of ethanol on mouse embryonic brain development and heat shock protein 73 expression.
Effects of ethanol on brain development and heat shock protein 70 (HSP70) expression were investigated in mouse embryos using the whole embryo culture, mid-brain culture, and streptavidin-biotin peroxidase complex (SABC) method. In the whole embryo culture, ethanol (1, 2 and 4 mg/ml) inhibited brain development dose dependently and the most prevalent abnormality was the open cephalic neural tube. At doses of 1 and 2 mg/ml, ethanol significantly decreased constitutive HSP70 (HSP73) expression level compared to control values in embryonic brain areas. In mid-brain culture, ethanol exposure (from 1 to 16 mg/ml) during early neuroblast differentiation inhibited neuronal differentiation and proliferation. These results suggest that ethanol may affect embryonic brain development by decreasing HSP73 expression level as well as inhibiting neuronal differentiation and proliferation during the organogenic period.
Jing
Hua
H
Department of Food Science and Nutrition, Laboratory of Molecular Toxicology and Developmental Molecular Biology, School of Public Health, Peking University, Beijing 100083, PR China. jinghua@bjmu.edu.cn
Li
Yong
Y
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008636:Mesencephalon / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D038081:Organogenesis / Q000187:drug effects* / Q000502:physiology
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494372:Hspa8 protein, mouse; D000431:Ethanol