Proteome analysis by bio-active ceramic water in rat liver: contribution to antioxidant enzyme expression, SOD I.
The purpose of this study was to investigate the protective effect of bio-active ceramic water on rat liver. Male Wistar rats were divided into 4 groups of 15 animals each. Groups 1 and 2 were fed bio-active ceramic water and tap water for 4 months, respectively. Groups 3 and 4 were treated with the same condition for 12 months. The changes of protein expression of these four groups were investigated using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Eleven proteins were significantly up-regulated in bio-active ceramic water treated rat liver including aldehyde dehydrogenase I and II, albumin, fructose-1,6-bisphosphatase, and superoxide dismutase I (SOD I). The most highly expressed protein, SOD I with up-regulated enzyme activity, was confirmed by immunoblots as a major antioxidant capable of detoxifying normally generated reactive oxygen species. These data suggest that modified protein expression of the liver contributes to enhance liver function.
Yun
Hae-Sun
HS
Department of Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Jeong
Won-Il
WI
Do
Sun-Hee
SH
Jeong
Da-Hee
DH
Jung
Yi-Rang
YR
Park
Jin-Kyu
JK
Cho
Eun-Mi
EM
Jeong
Kyu-Shik
KS
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D014867:Water / Q000008:administration & dosage*; D018508:Water Purification / Q000379:methods*
D000975:Antioxidants; D004798:Enzymes; D020543:Proteome; D014867:Water; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1