[Experimental study on regulatory effect of compound gengniankang on endocrine and immune functions in aged female rats with osteoporosis].
OBJECTIVE
To observe the effect of compound gengniankang (GNK) in regulating the endocrine and immune functions in aged female rats.


METHODS
Aged female rats with osteoporosis were selected as the object for observation and healthy young rats were taken for control. Animals were administered by GNK and nilestriol respectively, through gastric perfusion, for 3 months to observe the therapeutic effect of drug treatment on osteoporosis and the regulatory effect on endocrine and immune function. Bone mineral density (BMD) was measured by double energy X-ray absorption technique, serum levels of estradiol (E2), follicule-stimulating hormone (FSH) and luteinizing hormone (LH) were determined by RIA, T-cell subsets and apoptosis in spleen were detected by flow cytometry.


RESULTS
In aged rats with osteoporosis, the BMD decreased, serum level of E2 lowered, FSH and LH levels raised, splenic CD4+, CD4+/CD8+ significantly decreased and T-cell apoptosis rate significantly elevated. GNK could increase the BMD, lower the FSH and LH levels, but showed no significant effect on E2 level. It could increase the CD4+ and CD4+/CD8+ ratio to nearby the normal range, and reduce the apoptosis of T-cells.


CONCLUSIONS
GNK has therapeutic effect on osteoporosis in aged rats, and is able to regulate the endocrine and enhance the immune function in organism.
Wu
Su-hui
SH
Department of Obstetrics and Gynecology, the First Hospital of Shanxi Medical University, Taiyuan (030001). shwu1215@tom.com
Sun
Jing-fen
JF
Guo
Shu-zhen
SZ
D015502:Absorptiometry, Photon; D000375:Aging; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D010024:Osteoporosis / Q000097:blood* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000473:pathology
D004365:Drugs, Chinese Herbal; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone