Identification of some lectin IB4 binding proteins in rat dorsal root ganglia.
Lectins are proteins that bind to glycoproteins and glycolipids. The isolectin Griffonia simplicifolia I-B4 (IB4) recognizes terminal alpha-galactose and binds to a subset of small and medium-sized neurons in the dorsal root ganglia (DRG). Using one and two-dimensional gel electrophoresis, we have identified several proteins that bind IB4 in sciatic nerve, dorsal horn, and DRG. Treatment with the enzyme alpha-galactosidase reduces IB4 binding, strongly suggesting the binding is specific for the IB4 epitope. Mass spectrometric analysis of tryptic digests of alpha-galactosidase sensitive bands identified three proteins that bind IB4: the laminin beta 2 chain and the light and medium subunits of neurofilaments.
Fullmer
Joseph M
JM
Department of Neuroscience, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Ave, St. Paul, MN 55108, USA.
Riedl
Maureen S
MS
Higgins
LeeAnn
L
Elde
Robert
R
D000818:Animals; D005727:Ganglia, Spinal / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D031295:Griffonia; D008297:Male; D037121:Plant Lectins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D006023:Glycoproteins; C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins