title: RGM and its receptor neogenin regulate neuronal survival.
abstract: Repulsive guidance molecule (RGM) is an axon guidance protein that repels retinal axons upon activation of the neogenin receptor. To understand the functions of RGM-neogenin complexes in vivo, we used gene transfer technology to perturb their expression in the developing neural tube of chick embryos. Surprisingly, neogenin over-expression or RGM down-expression in the neural tube induces apoptosis. Neogenin pro-apoptotic activity in immortalized neuronal cells and in the neural tube is associated with the cleavage of its cytoplasmic domain by caspases. Thus neogenin is a dependence receptor inducing cell death in the absence of RGM, whereas the presence of RGM inhibits this effect.
authors.affiliation: UMR CNRS 7102, Université Paris 6, 9 Quai Saint Bernard, 75005 Paris, France.
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsunaga
authors.forename: Servane
authors.initials: S
authors.lastname: Tauszig-Delamasure
authors.forename: Philippe P
authors.initials: PP
authors.lastname: Monnier
authors.forename: Bernhard K
authors.initials: BK
authors.lastname: Mueller
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Strittmatter
authors.forename: Patrick
authors.initials: P
authors.lastname: Mehlen
authors.forename: Alain
authors.initials: A
authors.lastname: Chédotal
mesh_terms: D000818:Animals; D017209:Apoptosis; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D015536:Down-Regulation; D004789:Enzyme Activation; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats
chemical_list: D030161:Avian Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D034741:RNA, Small Interfering; C091279:neogenin; D049452:Green Fluorescent Proteins; D020169:Caspases