Characterization of a new C-type lectin from common carp Cyprinus carpio.
A new C-type lectin with putative mannose specificity was identified from the expressed sequence tag (EST) analysis of cDNA library from common carp head kidney (HK), stimulated with concanavalin A and lipopolysaccharide (LPS) during a routine EST analysis. The full sequence of 627 bp was identified by 5'-rapid amplification of cDNA ends. The gene is composed of 146 amino acid residues, including an 18-residue signal peptide for secretion and a single carbohydrate-recognition domain of approximately 118 residues typical of C-type lectins. Based on the predicted structure, this is a calcium dependent C-type lectin with putative mannose specificity suggested by the presence of an EPN motif. By hemagglutination assay, the mannose specificity of the Ca-CTL was determined. Reverse transcription (RT) PCR analysis demonstrated the expression of Ca-CTL mRNA in the hematopoietic organs and also the level of expression increased with LPS induction. Localization studies by in situ hybridization showed the presence of transcripts in the organs.
Savan
Ram
R
Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Endo
Makoto
M
Sakai
Masahiro
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002347:Carps / Q000378:metabolism*; D015723:Gene Library; D037181:Lectins, C-Type / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D037181:Lectins, C-Type