Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display.
SQAG9, a new class of immunosuppressive sulfoquinovosylacylglycerol, and its biotinylated derivatives have been synthesized. A T7 Phage library, composed of random cDNA fragments from Drosophila melanogaster, displayed a possible binding peptide of 14 amino acids. The immobilized synthetic peptide on a sensor chip showed a dissociation constant of K(D)=1.5 x 10(-6) against SQAG9 in a surface plasmon resonance experiment.
Yamazaki
Takayuki
T
Genome and Drug Research Center, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
Aoki
Satoko
S
Ohta
Keisuke
K
Hyuma
Shinji
S
Sakaguchi
Kengo
K
Sugawara
Fumio
F
D000818:Animals; D017123:Bacteriophage T7 / Q000235:genetics*; D004331:Drosophila melanogaster; D006017:Glycolipids / Q000138:chemical synthesis* / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000138:chemical synthesis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D006017:Glycolipids; D007166:Immunosuppressive Agents; D010455:Peptides; C496611:sulfoquinovosylacylglycerol