title: The LEF-1 high-mobility group domain undergoes a disorder-to-order transition upon formation of a complex with cognate DNA.
abstract: Lymphoid enhancer-binding factor-1 (LEF-1), a member of the high-mobility group (HMG) family of proteins, functions as an architectural transcription factor. In complex with its cognate DNA, the LEF-1 domain is highly ordered, and its NMR spectra are characteristic of a folded globular protein. In contrast, the uncomplexed protein exhibits NMR evidence of substantial conformational heterogeneity, although circular dichroism spectra indicate that much of the alpha-helical secondary structure of the DNA-bound state is retained in the free protein. Heteronuclear NMR experiments performed on the free LEF-1 domain reveal that helix II and helix III of the HMG domain are folded, although helix III is truncated at its C-terminal end relative to the DNA-bound protein. The major hydrophobic core between helices II and III appears to be formed, but the minor core near the C-terminus of helix III is unstructured in the free protein. Backbone resonances of helix I are undetectable, probably as a result of exchange broadening due to fluctuations between two or more conformations on a microsecond-to-millisecond time scale. On the basis of the circular dichroism spectrum, this region of the polypeptide appears to adopt helical structure but the helix is not fully stabilized in the absence of DNA. These findings argue that, prior to binding, bending, and distorting DNA, the HMG domain of LEF-1 exists in a segmentally disordered or partially folded state. Upon complex formation, the protein domain undergoes a cooperative folding transition with DNA to a highly ordered and well-folded state.
authors.affiliation: Department of Molecular Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Love
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: John
authors.initials: J
authors.lastname: Chung
authors.forename: H Jane
authors.initials: HJ
authors.lastname: Dyson
authors.forename: Peter E
authors.initials: PE
authors.lastname: Wright
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D024742:HMG-Box Domains; D006609:High Mobility Group Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007536:Isomerism; D051821:Lymphoid Enhancer-Binding Factor 1; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011392:Proline / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D012996:Solutions / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D010446:Peptide Fragments; D012996:Solutions; D014157:Transcription Factors; D004247:DNA; D011392:Proline