title: Demarcating the gene-rich regions of the wheat genome.
abstract: By physically mapping 3025 loci including 252 phenotypically characterized genes and 17 quantitative trait loci (QTLs) relative to 334 deletion breakpoints, we localized the gene-containing fraction to 29% of the wheat genome present as 18 major and 30 minor gene-rich regions (GRRs). The GRRs varied both in gene number and density. The five largest GRRs physically spanning <3% of the genome contained 26% of the wheat genes. Approximate size of the GRRs ranged from 3 to 71 Mb. Recombination mainly occurred in the GRRs. Various GRRs varied as much as 128-fold for gene density and 140-fold for recombination rates. Except for a general suppression in 25-40% of the chromosomal region around centromeres, no correlation of recombination was observed with the gene density, the size, or chromosomal location of GRRs. More than 30% of the wheat genes are in recombination-poor regions thus are inaccessible to map-based cloning.
authors.affiliation: Washington State University, Pullman, WA 99164, USA.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Erayman
authors.forename: Devinder
authors.initials: D
authors.lastname: Sandhu
authors.forename: Deepak
authors.initials: D
authors.lastname: Sidhu
authors.forename: Muharrem
authors.initials: M
authors.lastname: Dilbirligi
authors.forename: P S
authors.initials: PS
authors.lastname: Baenziger
authors.forename: Kulvinder S
authors.initials: KS
authors.lastname: Gill
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000032:analysis; D017343:Genes, Plant; D005819:Genetic Markers; D018745:Genome, Plant; D010641:Phenotype; D020161:Physical Chromosome Mapping; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers