title: Dengue virus surveillance: the co-circulation of DENV-1, DENV-2 and DENV-3 in the State of Rio de Janeiro, Brazil.
abstract: This study presents the results obtained in the monitoring of dengue virus (DENV) transmission in the Greater Metropolitan Region of the State of Rio de Janeiro, in the period 2000-2001. A total of 5324 serum samples from suspected cases of dengue were analysed in order to confirm dengue infection. The introduction of DENV-3 to the region in December 2000 resulted in the co-circulation of three serotypes: DENV-1, DENV-2 and DENV-3. In this study, virus isolation and/or reverse transcriptase PCR (RT-PCR) confirmed 52.3% (42/79) of DENV-3 cases, showing the importance of acute serum samples in the virological surveillance of the disease. Despite the introduction of a new serotype, an outbreak due to DENV-1 was observed in the municipality of Niteroi. The restriction site-specific PCR (RSS-PCR) patterns obtained for DENV-1 and DENV-2 isolated in that period showed that those strains belonged to the subtypes previously circulating in the state. DENV-3 RSS-PCR patterns confirmed that these viruses belonged to subtype C (Sri Lanka/India strains), represented by the strain circulating on the American continent. These data showed the importance of an active surveillance programme in countries where dengue is endemic.
authors.affiliation: Laboratory of Flavivirus, Department of Virology, Oswaldo Cruz Institute, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. tsimone@ioc.fiocruz.br
authors.forename: T S
authors.initials: TS
authors.lastname: De Simone
authors.forename: R M R
authors.initials: RM
authors.lastname: Nogueira
authors.forename: E S M
authors.initials: ES
authors.lastname: Araújo
authors.forename: F R
authors.initials: FR
authors.lastname: Guimarães
authors.forename: F B
authors.initials: FB
authors.lastname: Santos
authors.forename: H G
authors.initials: HG
authors.lastname: Schatzmayr
authors.forename: R V
authors.initials: RV
authors.lastname: Souza
authors.forename: G
authors.initials: G
authors.lastname: Teixeira Filho
authors.forename: M P
authors.initials: MP
authors.lastname: Miagostovich
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology; D001938:Brazil / Q000453:epidemiology; D002648:Child; D003715:Dengue / Q000097:blood / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D015994:Incidence; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011159:Population Surveillance / Q000379:methods; D012703:Serotyping / Q000379:methods; D017678:Sex Distribution; D014504:Urban Health
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M