Altered GABAB receptor immunoreactivity in the gerbil hippocampus induced by baclofen and phaclofen, not seizure activity.
The present study was performed to determine whether the effects induced by GABA(B) receptor-acting drugs would be related with the alteration in GABA(B) receptor expression in the hippocampus using Mongolian gerbil, a genetic epilepsy model. The distribution patterns of both GABA(B) receptor 1A/B and GABA(B)receptor 2 immunoreactivities were similarly detected in the hippocampi of normal and seizure-prone gerbils. Following baclofen (GABA(B) receptor agonist) or phaclofen (GABA(B) receptor antagonist) treatment, GABA(B) receptor immunoreactivities were decreased or increased by dose-dependent manners, respectively. Vigabatrin (GABA transaminase inhibitor) or 3-mercaptopropionic acid (GAD inhibitor) treatment did not affect GABA(B) receptor expressions. These findings suggest that GABA(B) receptor expression in the gerbil hippocampus may be altered by baclofen or phaclofen treatment.
Park
Seung-Kook
SK
Department of Anatomy, College of Medicine, Hallym University, Kangwon-do Chunchon 200-702, South Korea.
An
Sung-Jin
SJ
Hwang
In Koo
IK
Kim
Dae Won
DW
Jung
Ju-Young
JY
Won
Moo Ho
MH
Choi
Soo-Young
SY
Kwon
Oh-Shin
OS
Jeong
Young-Gil
YG
Kang
Tae-Cheon
TC
D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005849:Gerbillinae; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D018080:Receptors, GABA-B / Q000378:metabolism*; D020888:Vigabatrin / Q000494:pharmacology
C091727:3-mercaptoproline; D004791:Enzyme Inhibitors; D018756:GABA Antagonists; D018080:Receptors, GABA-B; C052329:phaclofen; D011392:Proline; D020888:Vigabatrin; D001418:Baclofen