title: Agrobacterium T-DNA integration: molecules and models.
abstract: Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule (T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host genome. Whereas extensive work has revealed the biological mechanisms governing the production, Agrobacterium-to-plant cell transport and nuclear import of the Agrobacterium T-DNA, the integration step remains largely unexplored, although several different T-DNA integration mechanisms have been suggested. Recent genetic and functional studies have revealed the importance of host proteins involved in DNA repair and maintenance for T-DNA integration. In this article, we review our understanding of the specific function of these proteins and propose a detailed model for integration.
authors.affiliation: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA. ttzvira@ms.cc.sunysb.edu
authors.forename: Tzvi
authors.initials: T
authors.lastname: Tzfira
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Li
authors.forename: Benoît
authors.initials: B
authors.lastname: Lacroix
authors.forename: Vitaly
authors.initials: V
authors.lastname: Citovsky
mesh_terms: D021581:Active Transport, Cell Nucleus; D004260:DNA Repair / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D008957:Models, Genetic; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA