Anti-genotoxicity of coffee against N-methyl-N-nitro-N-nitrosoguanidine in mouse lymphoma cells.
The main aim of this work was to test the hypothesis that instant coffee, a commonly consumed polyphenolic beverage with antioxidant activity, can protect mammalian cells against genotoxic effects in vitro. For this purpose, the L5178Y mouse lymphoma cell line was selected to assess modulatory effects of coffee on the genotoxicity of N-methyl-N-nitro-N-nitrosoguanidine (MNNG). We initiated the work with a set of preliminary experiments in which the cytokinesis-block micronucleus test was performed. Results obtained from these experiments demonstrated a dose-related decrease in genotoxicity following co-treatment of mouse lymphoma cells with three doses of caffeinated instant coffee. Both pre-treatment and co-treatment showed significant antigenotoxic effects against MNNG. Caffeinated and decaffeinated instant coffee samples inhibited genotoxicity. There was no significant change in the antigenotoxic effect of caffeinated instant coffee after filtration using a 0.2 microm filter. Similar in vitro experiments demonstrated antigenotoxic effects against MNNG when boiled coffee was used instead of instant coffee. On the basis of the findings from the above preliminary experiments, further work was carried out to evaluate the possible protective effects of caffeinated instant coffee against MNNG-induced DNA damage, mutation and chromosomal damage. Results from three or five independent experiments demonstrated significant protective effects of caffeinated instant coffee against MNNG-induced DNA damage in the comet assay, mutation at the Tk locus and chromosomal damage in the cytokinesis-block micronucleus test.
Abraham
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Stopper
H
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003069:Coffee / Q000737:chemistry*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005434:Flow Cytometry; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
D003069:Coffee; D020011:Protective Agents; D008769:Methylnitronitrosoguanidine