Pathophysiological significance of a reaction in mouse gastrointestinal tract associated with delayed-type hypersensitivity.
OBJECTIVE
To explore the pathophysiological significance of delayed type hypersensitivity (DTH) reaction in mouse gastrointestinal tract induced by an allergen 2,4-dinitrochlorobenzene (DNCB).


METHODS
BALB/c mice were randomly divided into control and DTH(1-6) groups. After sensitized by DNCB smeared on the abdominal skin, the mice were challenged with DNCB by gavage or enema. The weight, stool viscosity and hematochezia were observed and accumulated as disease active index (DAI) score; the gastrointestinal motility was represented by active charcoal propulsion rate; the colon pathological score was achieved by macropathology and HE staining of section prepared for microscopy; and the leukocyte migration inhibitory factor (LMIF) activity was determined by indirect capillary assay of the absorbance (A) of migrated leukocytes.


RESULTS
Active charcoal propulsion rates of small intestine in the DNCB gavages groups were significantly higher than that in the control group (P<0.01). The DAI scores and pathological score in DNCB enema groups were also higher than that in the control group (P<0.05), and there were significant rises in LMIF activity in DNCB enema groups as compared with control groups (P<0.01).


CONCLUSIONS
Mouse gastrointestinal DTH reaction could be induced by DNCB, which might facilitate the mechanism underlying the ulcerative colitis.
Yu
Wan-Gui
WG
Department of Physiology, Medical College of Yangtze University, Jingzhou, Hubei Province, China.
Lin
Ping
P
Pan
Hui
H
Xiao
Lan
L
Gong
En-Cong
EC
Mei
Lin
L
D000818:Animals; D004137:Dinitrochlorobenzene; D004342:Drug Hypersensitivity / Q000503:physiopathology*; D041981:Gastrointestinal Tract / Q000503:physiopathology*; D006968:Hypersensitivity, Delayed / Q000139:chemically induced* / Q000503:physiopathology*; D007509:Irritants; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D004137:Dinitrochlorobenzene; D007509:Irritants