Regulation of expression of mouse interferon-induced transmembrane protein like gene-3, Ifitm3 (mil-1, fragilis), in germ cells.
Mouse interferon-induced transmembrane protein (IFITM) gene, Ifitm3 (previously known as mil-1 and fragilis), is expressed in primordial germ cells (PGCs), in their precursors, and in germ cells of the fetal gonads (Saitou et al. [2002] Nature 418:293-300; Tanaka and Matsui [2002] Mech Dev 119S:S261-S267). By examining the expression of green fluorescent protein transgene under the control of DNA sequences flanking exon 1, we have identified domains that direct Ifitm3 transcription in PGCs and their precursors in gastrula stage and 13.5 days post coitum embryos. Germ cell-specific expression is achieved by the activity of a consensus element unique to the Ifitm genes, which may act to suppress Ifitm3 expression in somatic tissues. The lack of any influence of the interferon-stimulable response elements on transgene expression in the germ-line suggests that interferon-mediated response is not critical for activating Ifitm3.
Tanaka
Satomi S
SS
Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, Osaka, Japan. stanaka@cmri.usyd.edu.au
Nagamatsu
Go
G
Tokitake
Yuko
Y
Kasa
Miyuki
M
Tam
Patrick P L
PP
Matsui
Yasuhisa
Y
D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005091:Exons; D005260:Female; D005775:Gastrula; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005854:Germ Cells / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007372:Interferons / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid; D012737:Sex Factors; D013997:Time Factors; D019076:Transgenes
D020413:3' Untranslated Regions; C080082:IFITM3 protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D049452:Green Fluorescent Proteins; D004247:DNA; D007372:Interferons