Disruption of the RAD51 gene sensitizes S. cerevisiae cells to the toxic and mutagenic effects of hydrogen peroxide.
The RAD51 gene was disrupted in three different parental wild-type strains to yield three rad51 null strains with different genetic background. The rad51 mutation sensitizes yeast cells to the toxic and mutagenic effects of H2O2, suggesting that Rad51-mediated repair, similarly to that of RecA-mediated, is relevant to the repair of oxidative damage in S. cerevisiae. Moreover, pulsed-field gel electrophoresis analysis demonstrated that increased sensitivity of the rad51 mutant to H2O2 is accompanied by its decreased ability to repair double-strand breaks induced by this agent. Our results show that ScRad51 protects yeast cells from H2O2-induced DNA double-strand breakage.
Dudásová
Z
Z
Laboratory of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia.
Dudás
A
A
Alemayehu
A
A
Vlasáková
D
D
Marková
E
E
Chovanec
M
M
Vlcková
V
V
Brozmanová
J
J
D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000502:physiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051135:Rad51 Recombinase; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase