Increased plasma HDL cholesterol levels and biliary cholesterol excretion in hamster by LCAT overexpression.
Lecithin cholesterol acyltransferase (LCAT) is a key enzyme in the metabolism of high density lipoprotein (HDL), which has been found inversely correlated with atherosclerosis. Adenovirus mediated overexpression of human LCAT (hLCAT) in hamsters resulted in increased levels of plasma total cholesterol, HDL cholesterol, phospholipids and enlarged particle size of HDL. It also increased cholesterol and total bile acid concentrations in bile. Hepatic mRNA level of cholesterol 7alpha-hydroxylase increased 2.7-fold in hamsters. However, such effects were not observed in mice in a parallel experiment. This study suggests that overexpression of hLCAT in hamsters facilitated reverse cholesterol transport. Similar metabolic changes in humans might modify atherogenic risk.
Zhang
Ai-Hong
AH
Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiology, Ministry of Education, Peking University, 38 Xue Yuan Road, Beijing 100083, PR China.
Gao
Song
S
Fan
Jiang-Lin
JL
Huang
Wei
W
Zhao
Tie-Qiang
TQ
Liu
George
G
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001646:Bile / Q000378:metabolism*; D001692:Biological Transport; D015153:Blotting, Western; D002784:Cholesterol / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D050356:Lipid Metabolism; D008075:Lipoproteins, HDL / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D012333:RNA, Messenger; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase