Rapid and efficient identification of cysteine-rich peptides by random screening of a venom gland cDNA library from the hexathelid spider Macrothele gigas.
We identified novel 10 multi-cysteine peptides, namely Magi 7-16, from the spider Macrothele gigas by simple random cDNA screening of the venom gland. Mass analysis of the crude venom detected the mass numbers of the cross-linked forms of all peptides, confirming their presence in the venom. Magi 11, a C-terminus amidated peptide, was chemically synthesized and was indistinguishable from the native peptide proving the feasibility of the method for peptide identification. Moreover, toxicological assays showed diverse lethal or paralytic activities of these peptide toxins on mice and/or insects.
Satake
H
H
Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto-Cho, Mishima-Gun, Osaka 618-8503, Japan.
Villegas
E
E
Oshiro
N
N
Terada
K
K
Shinada
T
T
Corzo
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D003001:Cloning, Molecular; D003545:Cysteine; D005088:Exocrine Glands / Q000737:chemistry / Q000378:metabolism; D015723:Gene Library; D006135:Gryllidae / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013111:Spider Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D013112:Spiders
C492636:Magi toxin; D019151:Peptide Library; D010455:Peptides; D013111:Spider Venoms; D003545:Cysteine