Transmural distribution of iron in the hypoxic and reoxygenated rabbit left ventricular myocardium.
Transmural distribution of low molecular weight iron (LMWI), total iron, and protein carbonyls (PC) was investigated in the perfused rabbit heart under aerobic conditions, and after 60 min hypoxia followed or not by 3 min reoxygenation. In the aerobic perfused hearts, LMWI, total iron and PC did not show significant transmural differences. Hypoxia increased LMWI and PC levels, which were significantly higher in the subendocardium than in the subepicardium; further significant changes were not observed after reoxygenation. Total iron showed no transmural difference and was not significantly affected by both hypoxia and reoxygenation. Free iron was undetectable in the myocardial effluent of all experimental groups. Thus, hypoxia favors myocardial iron decompartmentalisation and oxidative stress, which are significantly greater in the inner than in the outer ventricular layers. Such findings may add further insight into the problem of the vulnerability of the mammalian subendocardium to injury induced by oxygen deprivation.
Lapenna
Domenico
D
Department of Medicine and Science of Aging, 'G. d'Annunzio' University School of Medicine and 'G. d'Annunzio' University Foundation, 66100, Chieti, Italy.
Ciofani
Giuliano
G
Pierdomenico
Sante D
SD
Di Gioacchino
Mario
M
Neri
Matteo
M
Giamberardino
Maria Adele
MA
Sabbioni
Enrico
E
Cuccurullo
Franco
F
D000818:Animals; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism*; D006439:Hemodynamics / Q000187:drug effects; D000860:Hypoxia / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D018384:Oxidative Stress; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion; D011817:Rabbits
D007501:Iron; D010100:Oxygen