[Effect of magnesium lithospermate B on endothelial cells in human aorta after free radical injury].
OBJECTIVE
To study the cardiovascular effects of magnesium lithospermate B (MLB), a water soluble extract from red sage root, on human aortic endothelial cells (HAECs) proliferation and in treating free radical injured endothelial cells, so as to further understand the cardiovascular pharmacological mechanism of MLB.


METHODS
HAECs of 3-6 passages were used in the experiment. MLB of different concentrations was used to treat the cells, and cell proliferation was observed using morphological and MTT method. The free radical injured model cell was made by glucose-glucose oxidase method for observing the effect of MLB on its microstructure by transmission electron microscopy.


RESULTS
MLB in concentration of 1.0 mg/ml and 0.5 mg/ml had significant cytotoxicity (P<0.01), when its concentration was lower than 0.2 mg/ml, the drug showed no adverse reaction on cell proliferation. MLB showed significantly cell protective effect against free radical injury, the effect in protecting ultrastructure of cells, such as mitochondria, could be demonstrated in transmission electron microscopy.


CONCLUSIONS
MLB showed a low cytotoxicity on HAECs, it could obviously protect cells from free radical injury.
Zhang
Zhi-xin
ZX
Department of Pediatrics, China-Japan Friendship Hospital, Beijing (100029). sunrj@mail.nsfc.gov.cn
Gao
Fu-yun
FY
Li
Ke-ming
KM
D001011:Aorta / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000473:pathology* / Q000648:ultrastructure; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; C061091:lithospermate B