The rat kidney acylase 1. Evidence for a new cDNA form and comparisons with the porcine intestinal enzyme.
A new cDNA form encoding the rat kidney acylase I was characterized and found to show as much as 93.5% identity in its translated nucleotide sequence and, to a lesser extent, in its 3'-untranslated region with the nucleotide sequence we previously reported in 2000. Comparisons between the amino acid sequences of the two corresponding proteins showed the presence of N-terminal fragments with 88.5% identity and different cysteine profiles. The cDNA nucleotide sequence of the pig intestinal enzyme isolated from a marathon library turned out to be 100% identical to that of the kidney enzyme, but differed from those of the two rat kidney acylase I forms.
Perrier
Josette
J
Institut Méditerranéen de Recherche en Nutrition, INRA-UMR 1111 service 342, Faculté des Sciences et Techniques Saint-Jérôme, Avenue Escadrille Normandie Niemen, F-13397 Marseille cedex 20, France. josette.perrier@univ.u-3mrs.fr
Durand
Anne
A
Giardina
Thierry
T
Puigserver
Antoine
A
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis* / Q000302:isolation & purification; D015723:Gene Library; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D013552:Swine
D018076:DNA, Complementary; D007527:Isoenzymes; D000581:Amidohydrolases; C019672:aminoacylase I