title: SIVmac Gag p27 capsid protein gene expression in potato.
abstract: A cDNA encoding the Simian immunodeficiency virus type (SIV(mac)) Gag capsid protein was introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation methods. The gag gene was detected in the genomic DNA of transformed leaf tissues by PCR DNA amplification. Immunoblot analysis of transformed potato plant extracts with anti-Gag monoclonal antibody showed that biologically active Gag protein was synthesized in transformed tuber tissues. Based on ELISA results, recombinant Gag protein made up 0.006-0.014% of total soluble tuber protein. The synthesis of SIV Gag in transformed potato tubers opens the way for development of Gag-based edible plant vaccines for protection against SIV and potentially HIV-1 infection.
authors.affiliation: Department of Biochemistry and Microbiology, Center for Molecular Biology and Gene Therapy, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: Tae-Geum
authors.initials: TG
authors.lastname: Kim
authors.forename: Ruth
authors.initials: R
authors.lastname: Ruprecht
authors.forename: William H R
authors.initials: WH
authors.lastname: Langridge
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000302:isolation & purification*; D016960:Agrobacterium tumefaciens / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics / Q000302:isolation & purification*; D015870:Gene Expression; D015683:Gene Products, gag / Q000235:genetics / Q000302:isolation & purification*; D015658:HIV Infections / Q000517:prevention & control*; D006801:Humans; D018449:SAIDS Vaccines / Q000235:genetics / Q000302:isolation & purification*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics
chemical_list: D016915:AIDS Vaccines; D036022:Capsid Proteins; D015683:Gene Products, gag; D018449:SAIDS Vaccines