Expression of receptor for advanced glycation end products (RAGE) and MMP-9 in human pancreatic cancer cells.
OBJECTIVE
Amphoterin is considered as a regulator for the ability of invasion and migration in tumor cells and embryonic neurons through binding to receptor for advanced glycation end products (RAGE), a multiligand cell surface molecule of the immunoglobulin superfamily. As matrix metalloproteinase-9 (MMP-9, gelatinase B) has been reported to play a critical role in tumor progression and metastasis, we have examined the relation of RAGE and MMP in human pancreatic cancer.


METHODS
Three representative human pancreatic carcinoma cells were rendered for the study which show different metastatic potential, PANC-1 and MIA PaCa-2 as the cells with high ability, BxPC-3 as with low. The expression of RAGE was examined by RT-PCR. The expression of MMP-9 protein was examined by Western blotting.


RESULTS
RAGE was strongly expressed in MIA PaCa-2 and PANC-1 that have high metastatic ability. On the contrary, RAGE was expressed little in BxPC-3 that has low ability. Similarly, expression of MMP-9 showed almost the same tendency. RAGE and MMP-9 are expressed concordant with the metastatic ability of the human pancreatic cancer cells.


CONCLUSIONS
Control of these molecules could be a key to regulating the metastatic ability of pancreatic cancer and this may be exploited in targeted therapy of this cancer.
Takada
Moriatsu
M
Department of Gastroenterological Surgery, Graduate School of Medicine, Kobe University, Kobe, Japan. moriatsu@med.kobe-u.ac.jp
Hirata
Kenro
K
Ajiki
Tetsuo
T
Suzuki
Yasuyuki
Y
Kuroda
Yoshikazu
Y
D015153:Blotting, Western; D045744:Cell Line, Tumor; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D020780:Matrix Metalloproteinase 9