Osmomechanics of the Propionigenium modestum F(o) motor.
In Propionigenium modestum, ATP is manufactured from ADP and phosphate by the enzyme ATP synthase using the free energy of an electrochemical gradient of Na+ ions. The P. modestum ATP synthase is a clear member of the family of F-type ATP synthases and the only major distinction is an extension of the coupling ion specificity to H+, Li+, or Na+, depending on the conditions. The use of Na+ as a coupling ion offers unique experimental options to decipher the ion-translocation mechanism and the osmotic and mechanical behavior of the enzyme. The single a subunit and the oligomer of c subunits are part of the stator and rotor, respectively, and operate together in the ion-translocation mechanism. During ATP synthesis, Na+ diffuses from the periplasm through the a subunit channel onto the Na+ binding site on a c subunit. From there it dissociates into the cytoplasm after the site has rotated out of the interface with subunit a. In the absence of a membrane potential, the rotor performs Brownian motions into either direction and Na+ ions are exchanged between the two compartments separated by the membrane. Upon applying voltage, however, the direction of Na+ flux and of rotation is biased by the potential. The motor generates torque to drive the rotation of the gamma subunit, thereby releasing tightly bound ATP from catalytic sites in F(1). Hence, the membrane potential plays a pivotal role in the torque-generating mechanism. This is corroborated by the fact that for ATP synthesis, at physiological rates, the membrane potential is indispensable. We propose a catalytic mechanism for torque generation by the F(o) motor that is in accord with all experimental data and is in quantitative agreement with the requirement for ATP synthesis.
Dimroth
P
P
Institut für Mikrobiologie, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland. micro.biol.ethz.ch
Matthey
U
U
Kaim
G
G
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001696:Biomechanical Phenomena; D017136:Ion Transport; D008564:Membrane Potentials; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000378:metabolism; D009995:Osmosis; D045184:Propionigenium / Q000201:enzymology*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D012964:Sodium / Q000378:metabolism
D020409:Molecular Motor Proteins; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D012964:Sodium; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases