Determination of allosteric interactions using radioligand-binding techniques.
Methods are presented for identifying and quantifying allosteric interactions of G-protein-coupled receptors with labeled and unlabeled ligands using radioligand-binding assays. The experimental designs and analyses are based on the simplest ternary complex allosteric model.
Lazareno
Sebastian
S
MRC Technology, London, United Kingdom.
D000494:Allosteric Regulation; D000818:Animals; D006801:Humans; D008024:Ligands; D011869:Radioligand Assay / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*
D008024:Ligands; D043562:Receptors, G-Protein-Coupled