Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation.
Androhep Plus, a long-term extender (up to 7 days) and Beltsville Thawing Solution (BTS), a short-term extender (up to 3 days), are commonly used for liquid storage of porcine semen. To test the hypothesis that modifications in sperm viability, motility, chlortetracycline (CTC) fluorescence patterns, and protein tyrosine phosphorylation occur during semen storage in extenders, we compared these end points at different periods of storage in either Androhep Plus or BTS. Sperm from five boars were assessed daily over 12 days of storage (n = 5 ejaculates from different boars). Viability was not different (P < 0.05 between extenders, except on Day 2, when Androhep Plus maintained better viability. Differences in the percentage of motile (total) sperm due to extender were evident on Days 2, 4, 5, and 6, when Androhep Plus was superior to BTS (P < 0.05). The percentages of progressively motile sperm also differed, with Androhep Plus supporting higher rates on Days 2, 4, 5, 7, 8, 9, 10, and 11 (P < 0.05). The CTC fluorescence pattern distribution differed due to extender as early as Day 2; storage in Androhep Plus induced higher levels of pattern B sperm (P < 0.05) than storage in BTS. A tyrosine-phosphorylated protein of Mr 21,000 appeared after 10 days in sperm incubated in BTS, and was identified as a phospholipid hydroperoxide glutathione peroxidase. Therefore, modifications in viability, motility, CTC fluorescence patterns, and sperm protein tyrosine phosphorylation were apparent during sperm storage in extenders; these may affect the fertilizing capacity of the semen.
Dubé
Charlotte
C
Département des Sciences Animales, Centre de Recherche en Biologie de la Reproduction, Pavillon Paul-Comtois, Université Laval, Sainte-Foy, Que., Canada G1K 7P4.
Beaulieu
Martin
M
Reyes-Moreno
Carlos
C
Guillemette
Christine
C
Bailey
Janice L
JL
D000818:Animals; D002470:Cell Survival; D002751:Chlortetracycline; D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes; D008297:Male; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012996:Solutions; D013075:Sperm Capacitation; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology*; D013552:Swine; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism*
D005456:Fluorescent Dyes; D010750:Phosphoproteins; D012996:Solutions; D014443:Tyrosine; D002751:Chlortetracycline