Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and genotype Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with Mycobacterial strains.
The performance of two DNA line probe assays, a new version of INNO-LiPA Mycobacteria (Innogenetics, Ghent, Belgium) and the GenoType Mycobacterium (Hain Diagnostika, Nehren, Germany), were evaluated for identification of mycobacterial species isolated from liquid cultures. Both tests are based on a PCR technique and designed for simultaneous identification of different mycobacterial species by reverse hybridization and line probe technology. The INNO-LiPA Mycobacteria v2 targeting the 16S-23S rRNA gene spacer region was developed for the simultaneous identification of 16 different mycobacterial species. The GenoType Mycobacterium, which targets the 23S rRNA gene, allows simultaneous identification of 13 mycobacterial species. Both tests were evaluated on 110 mycobacterial strains belonging to 22 different mycobacterial species (20 reference strains, 83 clinical strains, and 4 Mycobacterium kansasii strains isolated from tap water) that were previously inoculated into MB/BacT bottles. The sensitivity of both methods, defined as the number of positive results obtained with the Mycobacterium genus probe together with an interpretable result on the number of samples tested was 110 of 110 (100%) for INNO-LiPA and 102 of 110 (92.7%) for GenoType. For samples with interpretable results, INNO-LiPA was able to correctly identify 109 of 110 samples (99.1%), whereas the GenoType correctly identified 100 of 102 samples (98.0%). Both tests were easy to perform, rapid, and reliable when applied to mycobacterial identification directly from MB/BacT bottles.
Padilla
Eduardo
E
Servicio de Microbiología, Departamento de Genética y Microbiología, Hospital Universitario Germans Trias i Pujol, Universidad Autónoma de Barcelona, Ctra. del Canyet s/n, 08916 Badalona, Barcelona, Spain. epadilla.@ns.hugtip.scs.es
González
Victoria
V
Manterola
Jose María
JM
Pérez
Andrés
A
Quesada
María Dolores
MD
Gordillo
Sergio
S
Vilaplana
Cristina
C
Pallarés
María Angeles
MA
Molinos
Sonia
S
Sánchez
María Dolores
MD
Ausina
Vicente
V
D021903:DNA, Ribosomal Spacer; D005838:Genotype; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011934:Reagent Strips; D012680:Sensitivity and Specificity
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011934:Reagent Strips