title: Apo D in soft tissue tumors: a novel marker for dermatofibrosarcoma protuberans.
abstract: Using gene microarray expression profiling, we previously found that apolipoprotein D (Apo D) was highly expressed in dermatofibrosarcoma protuberans (DFSP). In this study, we confirm that Apo D is highly and relatively specifically expressed in DFSP using immunohistochemistry. A tissue microarray containing 421 soft tissue tumors was constructed and stained with antibodies against Apo D and CD34. Cytoplasmic immunostaining for Apo D was found in 9 of 10 typical DFSPs. In addition, 3 of 3 Bednar tumors and 2 of 3 giant cell fibroblastomas stained in conventional sections. In contrast, Apo D was immunoreactive in only a very small subset of a diverse collection of other soft tissue tumors, including Malignant Fibrous Histiocytoma (MFH), glomus tumor, neurofibroma, and malignant peripheral nerve sheath tumors. Immunostains for Apo D were negative in conventional sections of 16 fibrous histiocytomas, and an additional 12 variants of fibrous histiocytoma. Digital images of all immunohistochemical and hematoxylin and eosin tissue microarray stains are available at the accompanying website (http://microarray-pubs.stanford.edu/tma_portal/apod/). We conclude that Apo D is strongly expressed in DFSPs and neural lesions and may be useful in differentiating DFSP from fibrous histiocytoma.
authors.affiliation: Department of Pathology, Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA 94305, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: West
authors.forename: Jeff
authors.initials: J
authors.lastname: Harvell
authors.forename: Sabine C
authors.initials: SC
authors.lastname: Linn
authors.forename: Chih Long
authors.initials: CL
authors.lastname: Liu
authors.forename: Wijan
authors.initials: W
authors.lastname: Prapong
authors.forename: Tina
authors.initials: T
authors.lastname: Hernandez-Boussard
authors.forename: Kelli
authors.initials: K
authors.lastname: Montgomery
authors.forename: Torsten O
authors.initials: TO
authors.lastname: Nielsen
authors.forename: Brian P
authors.initials: BP
authors.lastname: Rubin
authors.forename: Rajiv
authors.initials: R
authors.lastname: Patel
authors.forename: John R
authors.initials: JR
authors.lastname: Goldblum
authors.forename: Patrick O
authors.initials: PO
authors.lastname: Brown
authors.forename: Matt
authors.initials: M
authors.lastname: van de Rijn
authors.forename: Chih Long
authors.initials: CL
authors.lastname: Lui
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001053:Apolipoproteins / Q000096:biosynthesis*; D053399:Apolipoproteins D; D014408:Biomarkers, Tumor / Q000378:metabolism; D018223:Dermatofibrosarcoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D040081:Protein Array Analysis; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D018952:Antigens, CD34; D001053:Apolipoproteins; D053399:Apolipoproteins D; D014408:Biomarkers, Tumor