Immune escape mechanism: defective resting and stimulated leukocyte-endothelium interaction in hepatocellular carcinoma of the rat.
Hepatocellular carcinoma represents an increasing therapeutic challenge due to its high incidence and early metastasis. Numerous studies have demonstrated the influence of the vascular system on tumor growth and development. In addition, the role of leukocyte-endothelium interaction in tumor vessels is of particular significance with regard to immunological tumor therapy. In this study we used an experimental in vivo animal model that allows a quantitative analysis on vessel morphology, microcirculation, and leukocyte-endothelium interaction. The vessel architecture in tumor tissue was found to be extremely heterogeneous, with a consecutively variable blood flow velocity. Following superfusion with chemotactic factors (fMLP, LB4), the leukocyte-endothelium interaction in tumor tissue with respect to leukocyte sticking was significantly reduced in comparison to healthy liver tissue. In conclusion, one of the main mechanisms for the reduced leukocyte-endothelium interaction in tumor tissue seems to be a decreased expression of adhesion molecules such as ICAM-1, indicating an effective immune escape mechanism for this tumor.
Maksan
Sasa-Marcel
SM
Department of Surgery, University of Mainz, Mainz, Germany. maksan@ach.klinik.uni-mainz.de
Araib
Patrick Morad
PM
Ryschich
Eduard
E
Gebhard
Martha Maria
MM
Schmidt
Jan
J
D000818:Animals; D001783:Blood Flow Velocity; D006528:Carcinoma, Hepatocellular / Q000503:physiopathology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002630:Chemotactic Factors / Q000502:physiology; D004730:Endothelium, Vascular / Q000503:physiopathology; D006439:Hemodynamics; D007962:Leukocytes / Q000502:physiology; D008099:Liver / Q000098:blood supply* / Q000503:physiopathology; D008113:Liver Neoplasms / Q000503:physiopathology*; D008297:Male; D008833:Microcirculation / Q000503:physiopathology*; D023421:Models, Animal; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011912:Rats, Inbred ACI
D015815:Cell Adhesion Molecules; D002630:Chemotactic Factors