Quantitative analysis of sinistrin in serum with high-performance liquid chromatography for renal function testing.
Sinistrin, as inulin, is widely used as a marker for renal function testing. A reliable and accurate method with a simple sample preparation for the quantitative determination of sinistrin would be of advantage. We developed a high-performance liquid chromatography (HPLC)-based method with electrochemical detection for the quantitative measurement of sinistrin in serum and plasma. Sample preparation is easy and includes enzymatic removal of glucose, deproteinization, acid hydrolysis of sinistrin, and HPLC separation of fructose. The recovery of sinistrin from serum is the same as that from water and is near 100%. The method presented has a linear range up to 500 mg/L. The results from our method are in agreement with a fully enzymatic quantification (regression coefficient 1.01, coefficient of variation 0.97). Sinistrin concentrations in aqueous solutions can be measured down to 2mg/L with a coefficient of variation of 5.7%. Quantification in serum is primarily limited by its physiological fructose content. The sensitivity of the described method is sufficient for its use in renal function testing. We describe a method for quantification of sinistrin which allows accurate measurements especially at low concentrations and low sample volumes. This laboratory method may be used for obtaining sinistrin pharmacokinetics in renal function testing.
Oettl
Karl
K
Institute of Medical Chemistry and Pregl Laboratory, Medical University Graz, Graz A-8010, Austria. karl.oettl@meduni-graz.at
Payerl
Doris
D
Zitta
Sabine
S
Müller
Thomas
T
Estelberger
Willibald
W
Reibnegger
Gilbert
G
D002374:Catalase / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D007668:Kidney / Q000502:physiology*; D009844:Oligosaccharides / Q000097:blood*; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry
D009844:Oligosaccharides; D011506:Proteins; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose; C064636:sinistrin