[The first polar body of human oocytes does not help predict the precise location of the spindle].
OBJECTIVE
To observe the precise position of the metaphase II spindle for facilitating clinical practice of assisted reproduction.


METHODS
LC-PolScope imaging system was used for observing the spindles in human metaphase II oocytes matured in vitro or in vivo, and the angle between the center of the oocyte, the spindle and the first polar body was measured and compared between in vitro and in vivo matured oocytes.


RESULTS
In vitro and in vivo matured oocytes were significantly different in the angle between the spindle and the first polar body (P=0.006).


CONCLUSIONS
The deviation of the spindle from the polar body in in vitro matured oocyte was significantly smaller than that in in vivo matured oocyte, and the first polar body does not help predict the precise location of the metaphase II spindle. The imaging system is safe and effective for observing the spindles.
Zhu
Liang
L
Assisted Reproductive Technology Center, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. zhu_liang75@sina.com
Kong
Ling-hong
LH
Li
Hong
H
Chen
Shi-Ling
SL
Xing
Fu-qi
FQ
D005260:Female; D006801:Humans; D008677:Metaphase; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000502:physiology*