title: Implantation of bone marrow mononuclear cells using injectable fibrin matrix enhances neovascularization in infarcted myocardium.
abstract: Neovascularization may improve cardiac function and prevent further scar tissue formation in infarcted myocardium. A number of studies have demonstrated that bone marrow-derived cells have the potential to induce neovascularization in ischemic tissues. In this study, we hypothesized that implantation of bone marrow mononuclear cells (BMMNCs) using injectable fibrin matrix further enhances neovascularization in infarcted myocardium compared to BMMNC implantation without matrix. To test this hypothesis, infarction was induced in rat myocardium by cryoinjury. Three weeks later, rat BMMNCs were mixed with fibrin matrix and injected into the infarcted myocardium. Injection of either BMMNCs or medium alone into infarcted myocardium served as controls. Eight weeks after the treatments, histological analyses indicated that implantation of BMMNCs using fibrin matrix resulted in more extensive tissue regeneration in the infarcted myocardium compared to BMMNC implantation without matrix. Examination with fluorescence microscopy revealed that cells labeled with a fluorescent dye prior to implantation survived in the infarcted myocardium at 8 weeks of implantation. Importantly, implantation of BMMNCs using fibrin matrix resulted in much more extensive neovascularization in infarcted myocardium than BMMNC implantation without matrix. The microvessel density in infarcted myocardium was significantly higher (p < 0.05) when BMMNCs were implanted using fibrin matrix (350 +/- 22 microvessels/mm2) compared to BMMNC implantation without matrix (262 +/- 13 microvessels/mm2) and medium injection (76 +/- 9 microvessels/mm2). In addition, average internal diameter of microvessels was significantly larger (p < 0.05) in BMMNC implantation with fibrin matrix group (14.6 +/- 1.2 microm) than BMMNC implantation without matrix group (10.2 +/- 0.7 microm) and medium injection group (7.3 +/- 0.5 microm). These results suggest that fibrin matrix could serve as a cell implantation matrix that enhances neovascularization efficacy for myocardial infarction treatment.
authors.affiliation: Department of Chemical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, South Korea.
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Ryu
authors.forename: Il-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Myeong-Chan
authors.initials: MC
authors.lastname: Cho
authors.forename: Kyung-Kuk
authors.initials: KK
authors.lastname: Hwang
authors.forename: Hainan
authors.initials: H
authors.lastname: Piao
authors.forename: Shuguang
authors.initials: S
authors.lastname: Piao
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lim
authors.forename: Yoo Sun
authors.initials: YS
authors.lastname: Hong
authors.forename: Cha Yong
authors.initials: CY
authors.lastname: Choi
authors.forename: Kyung Jong
authors.initials: KJ
authors.lastname: Yoo
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D016026:Bone Marrow Transplantation / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology; D005337:Fibrin / Q000008:administration & dosage*; D007267:Injections; D008833:Microcirculation / Q000473:pathology / Q000503:physiopathology; D009000:Monocytes / Q000473:pathology* / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology* / Q000601:surgery*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D005337:Fibrin