title: Objective and rapid assessment of pancreas graft viability using 31P-nuclear magnetic resonance spectroscopy combined with two- layer cold storage method.
abstract: BACKGROUND
  With the current shortage of donors, there is a critical need to optimally use "less-than-ideal" donors for pancreas transplantation. Objective and rapid means for assessing graft viability and suitability for transplantation are mandatory. This study examined the possibility of graft viability assessment and posttransplant outcome prediction using (31)P-nuclear magnetic resonance (NMR) spectroscopy combined with the two-layer cold-storage method (TLM).
  
  
  METHODS
  Canine pancreas grafts were preserved with TLM for 24 hours after 0, 60, or 120 minutes of warm ischemia (groups 1, 2, or 3, respectively). After preservation, we determined intragraft phosphate metabolites noninvasively using (31)P-NMR spectroscopy. Time required for this assessment was 5 minutes. Because our previous studies demonstrated that all grafts in groups 1 and 2 were successfully transplanted (the viable group), whereas all in group 3 failed to survive (the nonviable group), the possibility of posttransplant outcome prediction was examined on the basis of the comparison between these two groups.
  
  
  RESULTS
  The ratios of inorganic phosphate/gamma-adenosine triphosphate (Pi/gamma-ATP) and Pi/beta-ATP reflected the extent of graft damage, and the differences were statistically significant among groups 1, 2, and 3. On the basis of analyses of receiver operator characteristic curves, the optimum cutoff levels between the viable and nonviable groups were 1.6 and 2.2 for Pi/gammaATP and Pi/betaATP, respectively. The accuracy rates of these ratios were both 83%.
  
  
  CONCLUSIONS
  (31)P-NMR spectroscopy combined with TLM preservation could provide an objective, rapid, and possibly noninvasive means to assess pancreas graft viability and to determine suitability of damaged pancreata for organ transplantation.
authors.affiliation: Division of Gastroenterological Surgery, Department of Clinical Molecular Medicine, Graduate School of Medical Sciences, Kobe University, Kobe, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masaru
authors.initials: M
authors.lastname: Kanashiro
authors.forename: Shiri
authors.initials: S
authors.lastname: Li
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Goto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Keitaro
authors.initials: K
authors.lastname: Kakinoki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakai
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D004285:Dogs; D005260:Female; D006085:Graft Survival; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D016035:Pancreas Transplantation; D010759:Phosphorus Isotopes; D012680:Sensitivity and Specificity
chemical_list: D010759:Phosphorus Isotopes