title: Necessity's sharp pinch.
abstract: In a recent issue of Cell, Hughes and coworkers (Mnaimneh et al., 2004) provide a great leap forward in the analysis of essential yeast genes by constructing a strain set that expresses each essential gene from a tetracycline-regulatable promoter.
authors.affiliation: Department of Biochemistry and Molecular Biology, Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA.
authors.forename: Kristin T
authors.initials: KT
authors.lastname: Chun
authors.forename: Mark G
authors.initials: MG
authors.lastname: Goebl
mesh_terms: D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D020043:Genes, Essential; D005800:Genes, Fungal / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*; D014158:Transcription, Genetic
chemical_list: D011500:Protein Synthesis Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D013752:Tetracycline