Effects of tylosin use on erythromycin resistance in enterococci isolated from swine.
The effect of tylosin on erythromycin-resistant enterococci was examined on three farms; farm A used tylosin for growth promotion, farm B used tylosin for treatment of disease, and farm C did not use tylosin for either growth promotion or disease treatment. A total of 1,187 enterococci were isolated from gestation, farrowing, suckling, nursery, and finishing swine from the farms. From a subset of those isolates (n = 662), 59% (124 out of 208), 28% (80 out of 281), and 2% (4 out of 170) were resistant to erythromycin (MIC >/= 8 microg/ml) from farms A, B, and C, respectively. PCR analysis and Southern blotting revealed that 95% (65 out of 68) of isolates chosen from all three farms for further study were positive for ermB, but all were negative for ermA and ermC. By using Southern blotting, ermB was localized to the chromosome in 56 of the isolates while 9 isolates from farms A and B contained ermB on two similar-sized plasmid bands (12 to 16 kb). Pulsed-field gel electrophoresis revealed that the isolates were genetically diverse and represented a heterogeneous population of enterococci. This study suggests that although there was resistance to a greater number of enterococcal isolates on a farm where tylosin was used as a growth promotant, resistant enterococci also existed on a farm where no antimicrobial agents were used.
Jackson
Charlene R
CR
United States Department of Agriculture, Agricultural Research Service, Antimicrobial Resistance Research Unit, Richard B. Russell Research Center, Athens, GA 30605, USA. cjackson@saa.ars.usda.gov
Fedorka-Cray
Paula J
PJ
Barrett
John B
JB
Ladely
Scott R
SR
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology*; D015645:Tylosin / Q000494:pharmacology*
D001426:Bacterial Proteins; D004917:Erythromycin; C111078:ErmTR protein, bacteria; D008780:Methyltransferases; D015645:Tylosin