A mass spectrometry plate reader: monitoring enzyme activity and inhibition with a Desorption/Ionization on Silicon (DIOS) platform.
A surface-based laser desorption/ionization mass spectrometry assay that makes use of Desorption/Ionization on Silicon Mass Spectrometry (DIOS-MS) has been developed to monitor enzyme activity and enzyme inhibition. DIOS-MS has been used to characterize inhibitors from a library and then to monitor their activity against selected enzyme targets, including proteases, glycotransferase, and acetylcholinesterase. An automated DIOS-MS system was also used as a high-throughput screen for the activity of novel enzymes and enzyme inhibitors. On two different commercially available instruments, a sampling rate of up to 38 inhibitors per minute was accomplished, with thousands of inhibitors being monitored. The ease of applying mass spectrometry toward developing enzyme assays and the speed of surface-based assays such as DIOS for monitoring inhibitor effectiveness and enzyme activity makes it attractive for a broad range of screening applications.
Shen
Zhouxin
Z
Mass Consortium Corporation, 3030 Bunker Hill Street, San Diego, CA 92109, USA. zshen@massconsortium.com
Go
Eden P
EP
Gamez
Alejandra
A
Apon
Junefredo V
JV
Fokin
Valery
V
Greig
Mike
M
Ventura
Manuel
M
Crowell
John E
JE
Blixt
Ola
O
Paulson
James C
JC
Stevens
Raymond C
RC
Finn
M G
MG
Siuzdak
Gary
G
D004791:Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D012825:Silicon / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004791:Enzyme Inhibitors; D004798:Enzymes; D012825:Silicon