A kinetic model for Xylella fastidiosa adhesion, biofilm formation, and virulence.
Xylella fastidiosa is the causal agent of citrus variegated chlorosis and Pierce's disease which are the major threat to the citrus and wine industries. The most accepted hypothesis for Xf diseases affirms that it is a vascular occlusion caused by bacterial biofilm, embedded in an extracellular translucent matrix that was deduced to be the exopolysaccharide fastidian. Fourier transform infrared spectroscopy analysis demonstrated that virulent cells which form biofilm on glass have low fastidian content similar to the weak virulent ones. This indicates that high amounts of fastidian are not necessary for adhesion. In this paper we propose a kinetic model for X. fastidiosa adhesion, biofilm formation, and virulence based on electrostatic attraction between bacterial surface proteins and xylem walls. Fastidian is involved in final biofilm formation and cation sequestration in dilute sap.
Osiro
Denise
D
Embrapa Instrumentação Agropecuária, Rua XV de Novembro 1452, São Carlos SP 13560-970, Brazil.
Colnago
Luiz Alberto
LA
Otoboni
Alda Maria Machado Bueno
AM
Lemos
Eliana Gertrudes Macedo
EG
de Souza
Alessandra Alves
AA
Coletta Filho
Helvécio Della
HD
Machado
Marcos Antônio
MA
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D002957:Citrus / Q000382:microbiology; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D011134:Polysaccharides / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014774:Virulence; D027843:Vitis / Q000382:microbiology; D044167:Xylella / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011134:Polysaccharides