The mannitol cycle in Pleurotus ostreatus (Florida).
In mushroom, presence of the mannitol cycle has not been reported so far although the polyol is supposed to be generated by the reduction of fructose by mannitol dehydrogenase. This study submits evidence for the presence of the mannitol cycle in Pleurotus ostreatus. The key enzyme of the cycle, mannitol-1-phosphate dehydrogenase (M1PDH), was present appreciably in all the developmental stages of the mushroom. However, the enzyme level dropped significantly at the onset of sporulation. The presence of M1DPH was confirmed by isozyme analysis and RT-PCR mediated amplification of a approximately 400 bp DNA fragment.
Chakraborty
Tapas Kumar
TK
Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 054, India.
Basu
Debabrata
D
Das
Nirmalendu
N
Sengupta
Subhabrata
S
Mukherjee
Mina
M
D004591:Electrophoresis, Polyacrylamide Gel; D005631:Fructokinases / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism*; D009024:Morphogenesis; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013172:Spores, Fungal / Q000201:enzymology; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
D005656:Fungal Proteins; D009097:Multienzyme Complexes; D012331:RNA, Fungal; D008353:Mannitol; D013401:Sugar Alcohol Dehydrogenases; C021544:mannitol-1-phosphate dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D005631:Fructokinases; C046173:fructokinase; C034215:fructose-6-phosphatase; D010744:Phosphoric Monoester Hydrolases; C021543:mannitol-1-phosphatase