title: Application of polymethacrylate resin as stationary phase in liquid chromatography with UV detection for C1-C7 aliphatic monocarboxylic acids and C1-C7 aliphatic monoamines.
abstract: The application of unfunctionized polymethacrylate resin (TSKgel G3000PWXL) as a stationary phase in liquid chromatography with UV detection for C1-C7 aliphatic monocarboxylic acids (formic acid, acetic acid, propionic acid, butyric acid, isovaleric acid, valeric acid, 3,3-dimethylbutyric acid, 4-methylvaleric acid, hexanoic acid, 2-methylhexanoic acid, 5-methylhexanoic acid and heptanoic acid) and C1-C7 aliphatic monoamines (methylamine, ethylamine, propylamine, isobutylamine, butylamine, isoamylamine, amylamine, 1,3-dimethylbutylamine, hexylamine, 2-heptylamine and heptylamine) was carried out. Using dilute sulfuric acid as the eluent, the TSKgel G3000PWXL, resin acted as an advanced stationary phase for these C1-C7 carboxylic acids. Excellent simultaneous separation and symmetrical peaks for these C1-C7 carboxylic acids were achieved on a TSKgel G3000PWXL column (150 mm x 6 mm i.d.) in 60 min with 0.25 mM sulfuric acid containing 1 mM 2-methylheptanoic acid at pH 3.3 as the eluent. Using dilute sodium hydroxide as the eluent, the TSKgel G3000PWXL resin also behaved as an advanced stationary phase for these C1-C7 amines. Excellent simultaneous separation and good peaks for these C1-C7 amines were achieved on the TSKgel G3000PWXL column in 60 min with 10 mM sodium hydroxide containing 0.5 mM 1-methylheptylamine at pH 11.9 as the eluent.
authors.affiliation: Ceramics Research Institute, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora, Simoshidami, Moriyama-ku, Nagoya 463-8560, Japan. kazu.ohta@aist.go.jp
authors.forename: Kazutoku
authors.initials: K
authors.lastname: Ohta
authors.forename: Atsuya
authors.initials: A
authors.lastname: Towata
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohashi
authors.forename: Toyohide
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000588:Amines / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000588:Amines; D002264:Carboxylic Acids; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid