The anti-DNA knock-in model of systemic autoimmunity induced by the chronic graft-vs-host reaction.
The injection of spleen cells from bm12 mice into C57BL/6 recipients induces a chronic graft-vs-host reaction characterized by systemic autoimmunity, including anti-double-stranded DNA (anti-dsDNA) autoantibodies and immune complex-type proliferative glomerulonephritis. If the B6 recipient mice express an anti-DNA Vh site-directed transgene, the repertoire is skewed even more toward the anti-DNA response. Over a period of several weeks, high titers of serum anti-DNA antibodies appear and the mice develop renal damage. This permits the examination of the role of somatic immunoglobulin genetics and B-cell tolerance in a model of systemic lupus erythematosus.
Department of Medicine, University of Pennsylvania, Philadelphia, USA.
Robert
R
Eisenberg
Arpita
A
Choudhury
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis / Q000096:biosynthesis; D000921:Antibody-Producing Cells / Q000276:immunology; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D002908:Chronic Disease; D004247:DNA / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006087:Graft vs Host Reaction / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
D000974:Antibodies, Antinuclear; D004247:DNA