Protective activity of cedron (Aloysia triphylla) infusion over genetic damage induced by cisplatin evaluated by the comet assay technique.
Using the comet assay technique, this paper examines the protection from the cisplatin-induced genetic damage in mouse bone marrow cells provided by cedron-leaf infusion. Animals were separated into six groups: (I) untreated, (II) negative control, (III) treated with cedron-leaf infusion (5%), (IV) treated with cisplatin (6 mg/kg b.w.), (V) pretreated with infusion and treated with cisplatin and (VI) positive control (cyclophosphamide, 20 mg/kg b.w.). Based on the tail moment values found, four types of comets were distinguished. No statistical differences (P<0.01) were found between untreated animals, negative control and infusion treated mice. As expected, treatment of mice with a single dose of cis-DDP-induced genetic damage and the pretreatment with infusion prior to cis-DDP injection inhibited the capacity of cisplatin to induce genetic damage. Cell viability was up to 90% in all cases. The results suggest that infusion could exert its in vivo antigenotoxic action by enhancing the antioxidant status of bone marrow cells. The found could be attributed to its scavenging potency towards free radicals.
Laboratorio de Genética Toxicológica, GENETOX, Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad del Bío-Bío, Casilla 447, Chillán, Chile. ezamoran@ubiobio.cl
Enrique
E
Zamorano-Ponce
Julia
J
Fernández
Gilda
G
Vargas
Pilar
P
Rivera
Marta A
MA
Carballo
D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology*; D001854:Bone Marrow Cells; D002470:Cell Survival; D002945:Cisplatin / Q000009:adverse effects*; D020552:Comet Assay; D004249:DNA Damage; D005609:Free Radicals / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D029648:Verbenaceae / Q000737:chemistry*
D000970:Antineoplastic Agents; D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D002945:Cisplatin