Neutralization of venoms from two Southern Pacific Rattlesnakes (Crotalus helleri) with commercial antivenoms and endothermic animal sera.
The Southern Pacific Rattlesnake (Crotalus helleri) is found in southwestern California (USA), southward through north Baja California (MX) into the northern part of southern Baja California (MX). In this study, the venoms from two Southern Pacific Rattlesnakes were characterized. The two venoms were different in color, concentration, and enzyme activities. Two commercial antivenoms neutralized both C. helleri venoms differently. Antivipmyn (Fab2H) and CroFab (FabO) neutralized both venoms but had different ED50. Four times more Fab2H antivenom was required to neutralize the C. helleri venom No. 011-084-009 than the venom from the snake No. 010-367-284. The hemorrhagic activity of two C. helleri venoms were neutralized differently by endothermic animal sera having a natural resistance to hemorrhagic activity of snake venoms. Opossums and Mexican ground squirrel sera did not neutralize the hemorrhagic activity of the venom No. 010-367-284. The sera of gray woodrats and hispid cotton rats neutralized all hemorrhagins in both C. helleri venoms. This is the first reported case in which opossum serum has not neutralized hemorrhagic activity of pit viper venom. Differences in the compositions of C. helleri venoms and their ability to be neutralized may help explain why snakebites are a difficult medical problem to treat and why effective polyvalent antivenoms are difficult to produce.
Department of Biology, Natural Toxins Research Center (NTRC), Texas A&M University-Kingsville, MSC 158, Kingsville, TX 78363, USA.
Jacob A
JA
Galán
Elda E
EE
Sánchez
Alexis
A
Rodríguez-Acosta
John C
JC
Pérez
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D002140:California; D002852:Chromatography, Ion Exchange; D002863:Chromogenic Compounds / Q000378:metabolism; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors*; D017839:Crotalus; D004586:Electrophoresis; D005340:Fibrinogen / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006470:Hemorrhage / Q000139:chemically induced; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D007128:Immunoglobulin Fragments / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007928:Lethal Dose 50; D009500:Neutralization Tests; D009930:Organic Chemicals; D010974:Platelet Aggregation; D012377:Rodentia / Q000097:blood*; D044967:Serum / Q000378:metabolism*
D000997:Antivenins; D002863:Chromogenic Compounds; D003435:Crotalid Venoms; C431183:Crotalidae Polyvalent immune Fab; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D007328:Insulin; D009930:Organic Chemicals; C043626:hide powder azure; D005340:Fibrinogen; D018093:Gelatinases