G-CSF induced progenitor mobilization in mice with PIGA- blood cells.
OBJECTIVE
In patients with paroxysmal nocturnal hemoglobinuria (PNH) a proportion of blood cells are deficient in glycosyl phosphatidylinositol (GPI) anchored proteins due to a mutation in the PIGA gene. Previous studies showed that in PNH the majority of circulating early progenitor cells were normal but after G-CSF were mainly, of the PNH phenotype. This suggested that GPI-linked proteins contribute to the regulation of progenitor trafficking from bone marrow to peripheral blood.


METHODS
To test this hypothesis we studied progenitor cells in bone marrow, spleen, and peripheral blood in response to G-CSF in mice genetically engineered to have a proportion of blood cells deficient in GPI-linked proteins (LF mice).


RESULTS
In contrast to humans, LF and wild-type mice have comparable numbers of progenitor cells in bone marrow, spleen, and peripheral blood. Similarly, in LF mice the proportion of PIGA- progenitor cells in peripheral blood corresponds the proportion of PIGA- progenitor cells measured in bone marrow and spleen. After G-CSF the number of circulating progenitors significantly increased but the proportion of PIGA- cells remained the same in peripheral blood,bone marrow, and spleen.


CONCLUSIONS
Our data indicate that under basal laboratory conditions the lack of GPI-linked protein does not cause a retention of progenitor cells in the bone marrow. This implies that the preferential circulation of normal progenitor cells in patients with PNH requires an additional component that most likely is provided by the altered microenvironment of the underlying bone marrow failure.
Han
Bing
B
Division of Hematology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
Unsinger
Jacqueline
J
Liu
Fulu
F
Link
Dan C
DC
Bessler
Monica
M
D000818:Animals; D017261:Glycosylphosphatidylinositols / Q000097:blood*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000628:therapy*; D006801:Humans; D016130:Immunophenotyping; D008565:Membrane Proteins / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout
D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; C080086:phosphatidylinositol glycan-class A protein; D016179:Granulocyte Colony-Stimulating Factor