Crystallization and preliminary X-ray analysis of a bacterial glutathione transferase.
Crystals of a bacterial glutathione S-transferase from Proteus mirabilis have been grown from polyethylene glycol by the hanging-drop vapour-diffusion method. Successful crystallization required the presence of the substrate glutathione. The crystals belong to the tetragonal space group P4 with cell dimensions a = b = 90.9 and c = 117.3 A. They contain between three and six monomers in the asymmetric unit and diffract to beyond 2.3 A resolution.
The Ian Potter Foundation Protein Crystallography Laboratory, St. Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
S C
SC
Feil
M C
MC
Wilce
J
J
Rossjohn
N
N
Allocati
A
A
Aceto
C
C
Di Ilio
M W
MW
Parker