Ionizing radiation affects human MART-1 melanoma antigen processing and presentation by dendritic cells.
Radiation is generally considered to be an immunosuppressive agent that acts by killing radiosensitive lymphocytes. In this study, we demonstrate the noncytotoxic effects of ionizing radiation on MHC class I Ag presentation by bone marrow-derived dendritic cells (DCs) that have divergent consequences depending upon whether peptides are endogenously processed and loaded onto MHC class I molecules or are added exogenously. The endogenous pathway was examined using C57BL/6 murine DCs transduced with adenovirus to express the human melanoma/melanocyte Ag recognized by T cells (AdVMART1). Prior irradiation abrogated the ability of AdVMART1-transduced DCs to induce MART-1-specific T cell responses following their injection into mice. The ability of these same DCs to generate protective immunity against B16 melanoma, which expresses murine MART-1, was also abrogated by radiation. Failure of AdVMART1-transduced DCs to generate antitumor immunity following irradiation was not due to cytotoxicity or to radiation-induced block in DC maturation or loss in expression of MHC class I or costimulatory molecules. Expression of some of these molecules was affected, but because irradiation actually enhanced the ability of DCs to generate lymphocyte responses to the peptide MART-1(27-35) that is immunodominant in the context of HLA-A2.1, they were unlikely to be critical. The increase in lymphocyte reactivity generated by irradiated DCs pulsed with MART-1(27-35) also protected mice against growth of B16-A2/K(b) tumors in HLA-A2.1/K(b) transgenic mice. Taken together, these results suggest that radiation modulates MHC class I-mediated antitumor immunity by functionally affecting DC Ag presentation pathways.
Department of Radiation Oncology, Division of Surgical Oncology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Yu-Pei
YP
Liao
Chun-Chieh
CC
Wang
Lisa H
LH
Butterfield
James S
JS
Economou
Antoni
A
Ribas
Wilson S
WS
Meng
Keisuke S
KS
Iwamoto
William H
WH
McBride
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology / Q000528:radiation effects*; D000951:Antigens, Neoplasm; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology / Q000528:radiation effects*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D008213:Lymphocyte Activation; D058965:MART-1 Antigen; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D009363:Neoplasm Proteins / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D014161:Transduction, Genetic
D000951:Antigens, Neoplasm; D015395:Histocompatibility Antigens Class I; D058965:MART-1 Antigen; C548667:MLANA protein, human; C548668:Mlana protein, mouse; D009363:Neoplasm Proteins