Gene targeting: attention to detail.
When it comes to silencing genes in mice, not all approaches are equal. An example published in this issue of Cell (Patrucco et al., 2004) suggests that caution should be used when validating potential drug targets by genetic disruption.
Ludwig Institute for Cancer Research, London, United Kingdom.
Bart
B
Vanhaesebroeck
Jennifer L
JL
Rohn
Michael D
MD
Waterfield
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D018390:Gene Targeting; D051379:Mice; D005810:Multigene Family; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*
D000242:Cyclic AMP; D019869:Phosphatidylinositol 3-Kinases