[Effects of flavone from leaves of Diospyros kaki on adventitial fibroblasts proliferation by advanced oxidation protein products in vitro].
OBJECTIVE
To observe the effects of flavone from leaves of Diospyros kaki on adventitial fibroblasts proliferation by advanced oxidation protein products (AOPP) in vitro.


METHODS
NIH-3T3 cells were cultured in vitro and treated with AOPP and flavone from leaves of Diospyros kaki, respectively, and observed in comparison with the control group. The ratio of cell proliferation was determined by non-radioactive MTS/PES assay.


RESULTS
The ratio of cell proliferation was 1.789 +/- 0.299 in the control group, and 2.064 +/- 0.141, 2.149 +/- 0.218, 2.108 +/- 0.165, 2.124 +/- 0.131 and 2.087 +/- 0.125 in AOPP groups corresponding to AOPP concentrations of 100, 50, 10, 1 and 0.1 microg/ml, respectively. It showed that AOPP significantly induced the fibroblasts proliferation when the concentration was above 100 ng/ml (P < 0.05). The ratio of cell proliferation was 1.714 +/- 0.179 in flavone from leaves of Diospyros kaki group corresponding to concentration of 50 microg/ml. It also showed that flavone from leaves of Diospyros kaki alone had no effect on fibroblasts proliferation (P > 0.05). With AOPP stimulation, flavone from leaves of Diospyros kaki significantly inhibited fibroblasts proliferation (P < 0.05).


CONCLUSIONS
Flavone from leaves of Diospyros kaki can significantly inhibit the adventitial fibroblasts proliferation stimulated by AOPP in vitro.
Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Ping
P
Ouyang
Shangxi
S
Liu
Weijian
W
Bei
Wenyan
W
Lai
Fanfan
F
Hou
Anlong
A
Xu
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D029788:Diospyros / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D047309:Flavones; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D047309:Flavones; D005419:Flavonoids; D017127:Glycation End Products, Advanced; C043562:flavone