Robustness of the extraction step when parallel factor analysis (PARAFAC) is used to quantify sulfonamides in kidney by high performance liquid chromatography-diode array detection (HPLC-DAD).
The robustness of a multiresidue method has been analysed for the extraction and quantification of sulfamethoxypyridazine, sulfamethoxazole and sulfadimethoxine in porcine kidney by HPLC-DAD through a Plackett-Burman design. Two experimental responses were examined, the mean recovery from three replicates (accuracy) and their standard deviation (precision). Three factors were tested: the volume of phosphoric acid (pH) added in the extraction step, the time used for passing the sample through the solid-phase extraction cartridge (flow rate) and methanol volume to elute the analytes from the cartridge. Due to the non-specificity of the chromatograms (unknown matrix interferences coelute with each sulfonamide) the PARAFAC model was employed to evaluate the concentration recovered in the experiments of the Plackett-Burman design as well as to identify the spectra of the substances according to the criteria set in the European Decision 2002/657/EC for the analysis of residues. The extraction step was concluded to be robust to the recovery and the standard deviation of all three analytes.
García
Inmaculada
I
Department of Chemistry, Faculty of Sciences, University of Burgos, Pza. Misael Banuelos s/n, 09001 Burgos, Spain.
Sarabia
Luis
L
Ortiz
M Cruz
MC
Aldama
J Manuel
JM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005163:Factor Analysis, Statistical; D007668:Kidney / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
D013449:Sulfonamides