Determination of ciprofloxacin concentrations in human serum and urine by HPLC with ultraviolet and fluorescence detection.
OBJECTIVE
To develop a simple and sensitive high performance liquid chromatography method for the determination of ciprofloxacin concentrations in human serum and urine.


METHODS
Serum proteins were removed by ultrafiltration through a filtering device after the addition of a displacing reagent. Urine samples were diluted with mobile phase prior to injection. Separation was achieved with a C18 reverse-phase column and using ultraviolet (UVD) and fluorescence detection (FD) for serum samples and UVD for urine samples.


RESULTS
The quantitation limits of the assay were 20 ng/ml (FD) and 100 ng/ml (UVD) in serum and 1 microg/ml in urine. The assay was successfully applied to a pharmacokinetic study of ciprofloxacin in healthy volunteers.


CONCLUSIONS
The method presented for ciprofloxacin assay in human serum and urine requires less sample clean up and is more sensitive than those reported in the literature.
Department of Pharmacy Practice, School of Pharmacy and Pharmacal Sciences, Purdue University, Indianapolis, IN, USA. ksowinsk@iupui.edu
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Sowinski
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Kays
D000890:Anti-Infective Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D006801:Humans
D000890:Anti-Infective Agents; D002939:Ciprofloxacin