Flow process for electroextraction of intracellular enzymes from the fission yeast, Schizosaccharomyces pombe.
Flow treatment of the yeast, Schizosaccharomyces pombe, with high intensity electric field pulses released intracellular enzymes such as glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase. Over 70% of the total activity was liberated within 4 h after pulse application. The optimal field intensities were considerably higher than that needed for irreversible plasma membrane permeabilization.
Ganeva
V
V
Faculty of Biology, University of Sofia, 8 blvd Dragan Tzankov, 1421, Bulgaria.
Galutzov
B
B
Teissié
J
J
D002463:Cell Membrane Permeability / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D018274:Electroporation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000096:biosynthesis* / Q000302:isolation & purification*; D007700:Kinetics; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis* / Q000302:isolation & purification*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000528:radiation effects*
D005656:Fungal Proteins; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D010735:Phosphoglycerate Kinase