Effects of dopamine HCl on structural parameters of bovine brain membranes.
Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine.HCl on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), which were obtained from the bovine cerebral cortex. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, and radiationless energy transfer from the tryptophan of membrane proteins to Py-3-Py and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS) was also utilized. Dopamine.HCl increased both the bulk lateral mobility and annular lipid fluidity, and it had a greater fluidizing effect on the inner monolayer than on the outer monolayer. Furthermore, the drug had a clustering effect on membrane proteins.
College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Pusan 602-739, Korea.
Moon-Kyoung
MK
Bae
Min-Hoi
MH
Huh
Seung-Woo
SW
Lee
Hyun-Gu
HG
Kang
Jae-Ho
JH
Pyun
Myeong-Hee
MH
Kwak
Hye-Ock
HO
Jang
Il
I
Yun
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000648:ultrastructure; D004298:Dopamine / Q000494:pharmacology*; D005456:Fluorescent Dyes; D005470:Fluorometry; D066298:In Vitro Techniques; D008051:Lipid Bilayers; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000502:physiology; D011721:Pyrenes
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008563:Membrane Lipids; D011721:Pyrenes; C035116:1,3-di-(1-pyrenyl)propane; C027132:1-anilino-8-naphthalenesulfonate; D004298:Dopamine