Production of an anti-complement exo-polymer produced by Auricularia auricula-judae in submerged culture.
Exo-polymer (EP) was produced at 1.2 g l(-1) in submerged culture of Auricular auricula-judae. Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50). The activating pathway of the complement system occurred through both the classical and alternative pathways, though the major pathway was the classical one. Fractionation of AJ-0 using Sepharose CL-6B gel chromatography gave three major fractions (AJ-Fr-I, II and III) of which the first was the most active. The mycelial growth and EP production of A. auricula-judae were optimal at pH 6, 25 degrees C and pH 5, 25 degrees C, respectively.
Department of Biotechnology, Daegu University, Gyungsan, Gyungbuk 712-714, South Korea.
Sang-Chul
SC
Jeong
Sung-Pill
SP
Cho
Byung-Keun
BK
Yang
Young-Ah
YA
Gu
Jin-Ho
JH
Jang
Tae-Lin
TL
Huh
Chi-Hyun
CH
Song
D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods*; D003169:Complement Inactivator Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology*; D013149:Spiroplasma / Q000254:growth & development* / Q000378:metabolism*
D001704:Biopolymers; D003169:Complement Inactivator Proteins