Reclassification of Amycolatopsis mediterranei DSM 46095 as Amycolatopsis rifamycinica sp. nov.
Previous experiments have suggested that the rifamycin-producing strain DSM 46095 might not belong to Amycolatopsis mediterranei. Analysis of its 16S rRNA gene sequence and construction of a phylogenetic tree showed most similarity to Amycolatopsis kentuckyensis NRRL B-24129T, Amycolatopsis lexingtonensis NRRL B-24129T and Amycolatopsis pretoriensis NRRL B-24133T, but the strain was probably not a member of any of these species. Results from DNA-DNA hybridization experiments and comparison of DNA profiling patterns using pulsed-field gel electrophoresis also supported the assignment of strain DSM 46095 to a novel species. Analyses of phospholipids, fatty acid methyl esters and physiological characteristics also showed that the differences between different isolates of A. mediterranei and A. mediterranei DSM 46095 were as large as those between Amycolatopsis species. Strain DSM 46095 represents a novel species of the genus Amycolatopsis for which the name Amycolatopsis rifamycinica sp. nov. is proposed, with the type strain NT 19T (=DSM 46095T=ATCC 27643T).
Bala
Shashi
S
Department of Zoology, University of Delhi, Delhi 110007, India.
Khanna
Richie
R
Department of Zoology, University of Delhi, Delhi 110007, India.
Dadhwal
M
M
Department of Zoology, University of Delhi, Delhi 110007, India.
Prabagaran
S R
SR
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Shivaji
S
S
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Cullum
John
J
Department of Genetics, Kaiserslautern University of Technology, 67663 Kaiserslautern, Germany.
Lal
Rup
R
Department of Zoology, University of Delhi, Delhi 110007, India.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012294:Rifamycins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012294:Rifamycins