[Prostaglandin E1 reduces reperfusion injury of myocardium by inhibiting cytokines production during cardiac surgery].
To investigate the effects of prostaglandin E1 (PGE1) on the cytokines (IL-6, IL-8, IL-10) levels and ischemic -reperfusion injury of myocardium during cardiac surgery.



A total of 30 patients undergoing cardiac surgery (mitral valve replacement) under extracorporeal circulation were randomized into two groups: PGE1 group (receiving 0.04 microg.kg(-1). min(-1) of Lipo-PGE1 from the beginning of surgery to the end of study, n=15) and control group (no PGE1 given, n=15). Levels of serum IL-6, IL-8 and IL-10 were measured by enzyme-linked immunosorbent assays (ELISA). Isoenzyme of creatine kinase with muscle and brain subunits(CKMB) and troponin-T (cTn-T) were measured by ultraviolet absorption spectrophotometry method and enzyme immunoassay on 5 time-points during the study.



In both groups serum IL-6, IL-8, CK-MB and cTn-T levels increased significantly after aorta declamping (especially from 2 h after aorta declamping) compared with preoperative levels (P<0.05).However,the elevations of these cytokines and enzymes were more prominent in the control group than in the PGE1 group (P<0.05). Serum IL-10 concentration increased significantly from 2 h after aorta declamping compared with preoperative value (P<0.05); but there were no differences between the two groups. IL-6 and IL-8 levels were correlated with CK-MB and cTn-T concentrations (r2=0.40, r2=0.38 P>0.05 and r2=0.56, r2=0.14; P>0.05, respectively).



During cardiac surgery (mitral valve replacement) PGE1 may suppressed the production of IL-6, IL-8 but not IL-10, which may be related to its myocardial protection effect.
He
Zheng-fu
ZF
Department of Cardiothoracic Surgery, the Sir Run Run Shaw Hopital, College of Medicine, Zhejiang University, Hangzhou 310016, China. hezhengfu@21cn.com
Li
Zhi-jun
ZJ
D000527:Alprostadil / Q000627:therapeutic use*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008943:Mitral Valve / Q000601:surgery; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D012214:Rheumatic Heart Disease / Q000601:surgery; D020107:Troponin T / Q000097:blood
D015850:Interleukin-6; D016209:Interleukin-8; D020107:Troponin T; D016753:Interleukin-10; D000527:Alprostadil