Mapping of the Physcomitrella patens proteome.
The moss Physcomitrella patens is unique among land plants due to the high rate of homologous recombination in its nuclear DNA. The feasibility of gene targeting makes Physcomitrella an unrivalled model organism in the field of plant functional genomics. To further extend the potentialities of this seed-less plant we aimed at exploring the P. patens proteome. Experimental conditions had to be adopted to meet the special requirements connected to the investigations of this moss. Here we describe the identification of 306 proteins from the protonema of Physcomitrella. Proteins were separated by two dimensional electrophoresis, excised form the gel and analysed by means of mass spectrometry. This reference map will lay the basis for further profound studies in the field of Physcomitrella proteomics.
Sarnighausen
Eric
E
Plant Biotechnology, University of Freiburg, Schaenzlestr. 1, 79104 Freiburg, Germany. Eric.Sarnighausen@biologie.uni-freiburg.de
Wurtz
Virginie
V
Heintz
Dimitri
D
Van Dorsselaer
Alain
A
Reski
Ralf
R
D000595:Amino Acid Sequence; D019068:Bryopsida / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*
D010940:Plant Proteins; D020543:Proteome