Specific Cre/Lox recombination in the mouse proximal tubule.
The present work reports for the first time the construction of a transgenic mouse strain with specific expression of Cre recombinase in the kidney proximal tubule. A Cre/loxP strategy was developed using sglt2 promoter to drive Cre recombinase expression in transgenic mice. The mouse sglt2 5' region consisting of the first exon, the first intron, and part of the second exon was cloned upstream of a nucleotide sequence encoding the Cre recombinase. Transgenic mice were generated by pronuclear injection, and tissue specificity of Cre expression was analyzed using reverse transcription-PCR. The iL1-sglt2-Cre mouse line scored positive for kidney transcription of Cre but not for the other tissues analyzed. Within the kidney, Cre transcripts were demonstrated to be restricted to the proximal tubule only. iL1-sglt2-Cre mice were bred with ROSA26-LacZ reporter mice that contained a loxP-flanked stop sequence upstream of the LacZ gene. X-gal staining and immunohistochemistry using specific antibodies (anti-megalin, anti-Tamm-Horsfall, anti-NaCl co-transporter, and anti-aquaporin 2) revealed that sglt2 drives Cre functional expression specifically in proximal tubules. The iL1-sglt2-Cre mouse therefore represents a powerful tool for Cre-LoxP-mediated conditional expression in the renal proximal tubule.
UMR-CNRS 6548, University of Nice-Sophia Antipolis, Parc Valrose, 06109 Nice Cedex 2, France.
Isabelle
I
Rubera
Chantal
C
Poujeol
Guillaume
G
Bertin
Lilia
L
Hasseine
Laurent
L
Counillon
Philippe
P
Poujeol
Michel
M
Tauc
D000818:Animals; D005260:Female; D019426:Integrases / Q000235:genetics* / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008871:Microvilli / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051297:Sodium-Glucose Transporter 2; D019076:Transgenes / Q000235:genetics
D009004:Monosaccharide Transport Proteins; C490299:Slc5a2 protein, mouse; D051297:Sodium-Glucose Transporter 2; C045073:Cre recombinase; D019426:Integrases