Imaging glucose-regulated insulin secretion and gene expression in single islet beta-cells: control by AMP-activated protein kinase.
The mechanisms by which changes in glucose concentration regulate gene expression and insulin secretion in pancreatic islet beta-cells are only partly understood. Here we describe the development of new technologies for examining these processes at the level of single living beta-cells. We also present recent findings, made using these and other techniques, which implicate a role for adenosine 5'-monophosphate-activated protein kinase in glucose signaling in these cells.
Rutter
Guy A
GA
Henry Wellcome Laboratories of Integrated Cell Signalling and Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, United Kingdom. g.a.rutter@bris.ac.uk
Leclerc
Isabelle
I
Tsuboi
Takashi
T
Xavier
Gabriela Da Silva
Gda S
Diraison
Frédérique
F
Qian
Qingwen
Q
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D007328:Insulin; D049452:Green Fluorescent Proteins; D019869:Phosphatidylinositol 3-Kinases; D055372:AMP-Activated Protein Kinases; D005947:Glucose