Regulation of fibrillin-1 by biglycan and decorin is important for tissue preservation in the kidney during pressure-induced injury.
There is growing evidence that the two small leucine-rich proteoglycans biglycan and decorin regulate the assembly of connective tissues and alter cell behavior during development and pathological processes. In this study, we have used an experimental animal model of unilateral ureteral ligation and mice deficient in either biglycan or decorin. We discovered that pressure-induced injury to the wild-type kidneys led to overexpression of decorin, biglycan, fibrillin-1, and fibrillin-2. In contrast, in biglycan-deficient kidneys the overexpression of fibrillin-1 was markedly attenuated and this was associated with cystic dilatation of Bowman's capsule and proximal tubules. Notably, we found that in ligated kidneys from decorin-null mice, fibrillin-1 expression was initially enhanced to the same extent as in wild-type animals. However, long-term obstruction resulted in down-regulation of fibrillin-1 and concurrent cystic dilatation of Bowman's capsule in 33% of kidneys at 5 months after obstruction. In all of the genotypes, no differences in fibrillin-2 expression were observed. These in vivo data correlated with a significant induction of fibrillin-1 expression in renal fibroblasts and mesangial cells by recombinant biglycan and decorin. Our results indicate a novel role for decorin and biglycan during pressure-induced renal injury by stimulating fibrillin-1 expression.
Schaefer
Liliana
L
Medizinische Klinik und Poliklinik D, Albert-Schweitzer-Str. 33, 48149 Münster, Germany. schaefl@uni-muenster.de
Mihalik
Daniel
D
Babelova
Andrea
A
Krzyzankova
Miroslava
M
Gröne
Hermann-Josef
HJ
Iozzo
Renato V
RV
Young
Marian F
MF
Seidler
Daniela G
DG
Lin
Guoqing
G
Reinhardt
Dieter P
DP
Schaefer
Roland M
RM
D000818:Animals; D058578:Biglycan; D058575:Decorin; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D000071838:Fibrillin-1; D000071840:Fibrillin-2; D000071837:Fibrillins; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005355:Fibrosis / Q000235:genetics / Q000473:pathology; D005786:Gene Expression Regulation; D005838:Genotype; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism; D006579:Heterozygote; D006720:Homozygote; D007668:Kidney / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000378:metabolism*; D011312:Pressure / Q000009:adverse effects; D011509:Proteoglycans / Q000235:genetics / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D054392:Adipokines
D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; D000071838:Fibrillin-1; D000071840:Fibrillin-2; D000071837:Fibrillins; D008840:Microfilament Proteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; C546885:Bgn protein, mouse; C546879:Dcn protein, mouse; C000605724:Fbn1 protein, mouse; D054392:Adipokines; C000605728:Fbn2 protein, mouse