Rapid identification of stress-related fingerprint from whole bacterial cells of Bifidobacterium lactis using matrix assisted laser desorption/ionization mass spectrometry.
Whole cells of Bifidobacterium lactis were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Characteristic and reproducible mass spectra were obtained in the mass range from 6 to 19 kDa. After several days of bacterial cell storage at 4 degrees C (D0, D2, and D6), only minor signal differences were observed. Under identical and reproducible conditions, fourteen relevant diagnostic ions were identified. Moreover, control- and stress-related fingerprints were rapidly obtained using MALDI-TOFMS by comparison of protein patterns obtained from non-stressed (control) versus stressed cells (addition of bile salts during growth). After quantitative validation of the MALDI-MS data by a statistical approach, two and eight signals were assigned as control- and stress-specific ions, respectively. This work provides the evidence that MALDI-TOFMS can be used for the identification of stress-related fingerprint of B. lactis bacterial cells and could have a high potential for the assessment of the physiological status of the cells.
Marvin-Guy
Laure F
LF
Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland. laure.marvin-guy@rdls.nestle.com
Parche
Stephan
S
Wagnière
Sandrine
S
Moulin
Julie
J
Zink
Ralf
R
Kussmann
Martin
M
Fay
Laurent B
LB
D001644:Bifidobacterium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D001647:Bile Acids and Salts / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D010449:Peptide Mapping; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001647:Bile Acids and Salts