A novel potassium deficiency-induced stimulon in Anabaena torulosa.
Potassium deficiency enhanced the synthesis of fifteen proteins in the nitrogen-fixing cyanobacterium Anabaena torulosa and of nine proteins in Escherichia coli. These were termed potassium deficiency-induced proteins or PDPs and constitute hitherto unknown potassium deficiency-induced stimulons. Potassium deficiency also enhanced the synthesis of certain osmotic stress-induced proteins. Addition of K+ repressed the synthesis of a majority of the osmotic stress-induced proteins and of PDPs in these bacteria. These proteins contrast with the dinitrogenase reductase of A. torulosa and the glycine betaine-binding protein of E. coli, both of which were osmo-induced to a higher level in potassium-supplemented conditions. The data demonstrate the occurrence of novel potassium deficiency-induced stimulons and a wider role of K+ in regulation of gene expression and stress responses in bacteria
Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Anuradha
A
Alahari
Shree Kumar
SK
Apte
D017033:Anabaena / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D017037:Dinitrogenase Reductase / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D009997:Osmotic Pressure; D033902:Periplasmic Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D011188:Potassium / Q000494:pharmacology / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects
D001426:Bacterial Proteins; D017037:Dinitrogenase Reductase; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C053437:PROX protein, E coli; D033902:Periplasmic Binding Proteins; D011188:Potassium