Murine B7-H3 is a negative regulator of T cells.
T cell activation is regulated by the innate immune system through positive and negative costimulatory molecules. B7-H3 is a novel B7-like molecule with a putative receptor on activated T cells. Human B7-H3 was first described as a positive costimulator, most potently inducing IFN-gamma production and cellular immunity. In this study we examined the expression and function of mouse B7-H3. B7-H3 is mostly expressed on professional APCs; its expression on dendritic cells appears to be up-regulated by LPS. In contrast to human B7-H3, we found that mouse B7-H3 protein inhibited T cell activation and effector cytokine production. An antagonistic mAb to B7-H3 enhanced T cell proliferation in vitro and led to exacerbated experimental autoimmune encephalomyelitis in vivo. Therefore, mouse B7-H3 serves as a negative regulator of T cell activation and function.
Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Durbaka V R
DV
Prasad
Thang
T
Nguyen
Zhaoxia
Z
Li
Yang
Y
Yang
Julie
J
Duong
Ying
Y
Wang
Chen
C
Dong
D000818:Animals; D019138:Antibodies, Blocking; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D060887:B7 Antigens; D018122:B7-1 Antigen / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*
D019138:Antibodies, Blocking; D060887:B7 Antigens; D018122:B7-1 Antigen; C486038:Cd276 protein, mouse; D016207:Cytokines