Inhibitory effect of polypeptide from Chlamys farreri on UVA-induced apoptosis in human keratinocytes.
Polypeptide from Chlamys farreri (PCF, Mr = 879) is a novel marine active product isolated from gonochoric Chinese scallop Chlamys farreri which has been served as sea food for several thousand years. As an octapeptide, PCF consists of 8 amino acids, namely, Pro, Asn, Ser, Thr, Arg, Hyl, Cys, and Gly. PCF had been identified as a marine chemopreventive drug that protected hairless mice's epidermis against UV-induced damage in our previous study. However, the molecular mechanisms that underlie the effect of PCF on ultraviolet A-induced apoptosis in ketatinocytes are not well understood yet. In the present study, PCF was investigated as a potential inhibitory agent for UVA-induced apoptosis in a human keratinocyte cell line, HaCaT. The effects of PCF on UVA-induced generation of ROS and MDA, DNA damage, apoptosis rate were examined. We also investigated whether PCF could inhibit UVA-induced decreasing of mitochondrial membrane potential and the changing of morphology of the cells. We found that, compared with UVA only group, PCF attenuated UVA-induced generation of ROS and MDA, increased the mitochondrial membrane potential, and decreased the apoptosis rate. These results indicate that PCF may protect HaCaT keratinocytes against UVA-induced apoptosis.
Dou
Mei
M
Department of Pharmacology, Medical College of Qingdao University, Qingdao, China.
Han
YanTao
Y
Han
ZhiWu
Z
Chen
XiangGuo
X
Wang
YueJun
Y
Tan
JinShan
J
Wang
ChunBo
C
Hou
YingYi
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D020552:Comet Assay; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D008315:Malondialdehyde / Q000037:antagonists & inhibitors; D008564:Membrane Potentials / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D008974:Mollusca; D009842:Oligopeptides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D014466:Ultraviolet Rays / Q000009:adverse effects*
D009842:Oligopeptides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde