Poly-N-acetyl glucosamine-mediated red blood cell interactions.
BACKGROUND
Investigations were performed to assess the effect of poly-N-acetyl glucosamine (p-GlcNAc) fiber slurry-mediated hemostasis by interactions with red blood cells.


METHODS
Red blood cell aggregation studies were performed using test material-coated microscope slides and multiphoton microscopic measurements. Enzymatic removal of red blood cell surface proteins was achieved using trypsin and neuraminidase treatment. Zeta-potential measurements (surface charge) were performed.


RESULTS
Red blood cells interact directly with poly-N-acetyl glucosamine polymers through ionic interactions and cell-surface proteins. The effective concentration of poly-N-acetyl glucosamine fiber material for 50% red blood cell aggregation was 0.28 mg/mL. The p-GlcNAc beta-configuration fibers and an alpha-configuration structural modification of the fibers both produced maximal responses because of their zeta-potentials, whereas other chemically modified p-GlcNAcs and chitosans were ineffective.


CONCLUSIONS
Poly-N-acetyl glucosamine-induced red blood cell aggregation is mediated by interactions with red blood cell surface charges.
Department Surgery, VA Boston Healthcare System, Brigham and Women's Hospital, Harvard Medical Schools, Boston, MA, USA. hemant_thatte@hms.harvard.edu
Hemant S
HS
Thatte
Sofija E
SE
Zagarins
Mansoor
M
Amiji
Shukri F
SF
Khuri
D000117:Acetylglucosamine / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan; D004353:Drug Evaluation, Preclinical; D004586:Electrophoresis; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006400:Hematocrit; D006488:Hemostasis, Surgical / Q000379:methods; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007477:Ions; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects; D036641:Microscopy, Fluorescence, Multiphoton; D055095:Optics and Photonics; D010973:Platelet Adhesiveness / Q000187:drug effects; D012995:Solubility; D013053:Spectrophotometry; D013499:Surface Properties / Q000187:drug effects
D006490:Hemostatics; D007477:Ions; D008565:Membrane Proteins; C113831:poly-N-acetyl glucosamine; D002686:Chitin; D048271:Chitosan; D000117:Acetylglucosamine