POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer.
DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.
The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA. clausted@systemsbiology.org
Christopher
C
Lausted
Timothy
T
Dahl
Charles
C
Warren
Kimberly
K
King
Kimberly
K
Smith
Michael
M
Johnson
Ramsey
R
Saleem
John
J
Aitchison
Lee
L
Hood
Stephen R
SR
Lasky
D004271:DNA, Fungal / Q000235:genetics; D005740:Gases; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D009038:Motion; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000295:instrumentation*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D012997:Solvents; D013997:Time Factors; D015003:Yeasts / Q000145:classification / Q000235:genetics
D004271:DNA, Fungal; D005740:Gases; D009841:Oligonucleotides; D012997:Solvents