[RAPD analysis on different isolates of Trichomonas vaginalis].
OBJECTIVE
To study genetic polymorphism of DNA on seven isolates of Trichomonas vaginalis.


METHODS
The random amplified polymorphic DNA (RAPD) technique was performed to amplify genomic DNA of the seven T. vaginalis isolates, including Beijing 1, Beijing 2, Chengde, Tangshan, Jiujiang 1, Jiujiang 2 and Jiujiang 3. The DNA bands detected were analyzed by clustering analysis with SPSS software.


RESULTS
The percentage of genetic similarity among the seven isolates was from 77.4% to 94.7%, showing a close genetic relationship among them. The percentages between the isolates of Beijing 1 and Tangshan, Jiujiang 1 and Jiujiang 2, Beijing 2 and Jiujiang 3 were 89.2%, 92.1% and 94.7% respectively, while that of Jiujiang 1 and Chengde was 77.4%, indicating a lower homology.


CONCLUSIONS
There are a close genetic relationship and certain gene polymorphism among the seven T. vaginalis isolates; geographical origin plays little role to the genetic characteristics.
Department of Biology, North China Coal Medical College, Tangshan 063000, China.
Li-jie
LJ
Yuan
Xing-zheng
XZ
Gao
D000818:Animals; D002681:China; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D006801:Humans; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D016054:DNA, Protozoan