A powerful selection assay for mixture libraries of DNA alkylating agents.
A simple and powerful selection assay that permits the separation (rpHPLC), quantitation (ELSD), and identification (ESI-MS) of thermally released adenine adducts derived from duocarmycin analogues is detailed that can establish the most effective DNA alkylating agents in synthetic combinatorial mixtures.
Ham
Young-Wan
YW
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Boger
Dale L
DL
D000477:Alkylating Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000478:Alkylation / Q000187:drug effects; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D000080890:Duocarmycins; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology; D011760:Pyrrolidinones / Q000737:chemistry / Q000494:pharmacology
D000477:Alkylating Agents; D000080890:Duocarmycins; D007211:Indoles; D011758:Pyrroles; D011760:Pyrrolidinones; C066098:duocarmycin SA; D004247:DNA; C058289:duocarmycin A