Characterization of the epitopes specific for the monoclonal antibody 9F5-3a and quantification of oxidized HDL in human plasma.
BACKGROUND
We previously isolated a monoclonal antibody, 9F5-3a, that is specific for HDL oxidized by CuSO(4).


METHODS
We examined the characteristics of the 9F5-3a epitope by Western blot and measured the concentration of oxidized HDL in human plasma by enzyme-linked immunosorbent assay using this antibody.


RESULTS
The monoclonal antibody specifically reacted with oxidized HDL in a mixture of HDL, LDL and modified lipoproteins. Oxidation of the HDL particles accelerated cross-linkage of apolipoproteins caused by lipid peroxidation, and the cross-linked apolipoprotein AI selectively reacted with the 9F5-3a antibody. Mean (standard deviation) plasma concentrations of oxidized HDL were 127 (50) microg/L in 23 healthy controls, 191 (65) microg/L in 30 patients with non-insulin-dependent diabetes mellitus (P < 0.01 versus healthy controls) and 200 (87) microg/L in 25 patients with coronary artery disease (P < 0.01 versus healthy controls). The concentrations of oxidized HDL did not correlate with the concentrations of thiobarbituric acid-reactive substances.


CONCLUSIONS
The results indicate that determination of oxidized HDL concentration may be useful for identifying patients with atherosclerotic disease.
Nakajima
Takanori
T
Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Saitama 350-0495, Japan.
Matsunaga
Toshiyuki
T
Kawai
Shin-Ichiroh
S
Hokari
Shigeru
S
Inoue
Ikuo
I
Katayama
Shigehiro
S
Nagata
Atsuo
A
Komoda
Tsugikazu
T
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008075:Lipoproteins, HDL