A gel-mediated feeding technique for protein crystal growth from hanging drops.
A procedure which allows an investigator to supply a crystal with fresh mother material without inducing significant growth defects is described. This technique requires that the crystal is grown in a gelled hanging or sitting drop. An example concerning a model macromolecule, hen egg-white lysozyme, is given. Extension of this procedure to other macromolecules is discussed.
Bernard
Y
Y
Laboratoire de Minéralogie-Cristallographie, associé au CNRS et aux Universités Paris 6 et 7, France.
Degoy
S
S
Lefaucheux
F
F
Robert
M C
MC