Effect of voluntary exercise on genetically obese Cpefat/fat mice: quantitative proteomics of serum.
OBJECTIVE
To compare the effect of voluntary exercise on body weight, food consumption, and levels of serum proteins between wild-type and carboxypeptidase E-deficient (Cpefat/fat) mice.


METHODS
Study 1 consisted of three groups of female mice: Cpefat/fat mice with continuous access to exercise wheels for 3 weeks (n = 4); wild-type C57BKS mice with access to exercise wheels for 3 weeks (n = 4); and sedentary Cpefat/fat mice (n = 3). Activity, body weight, and food consumption were monitored for this period and a subsequent 9-week period without exercise wheels. Study 2 consisted of four groups of male mice (n = 6 to 7 each): Cpefat/fat mice with exercise wheels, wild-type mice with exercise wheels, and Cpefat/fat and wild-type mice without exercise wheels. Body weight and food consumption were measured over 4 weeks. Sera were collected, and the protein profile was determined by 2-dimensional gel electrophoresis and mass spectrometry.


RESULTS
Cpefat/fat mice were moderately hyperphagic but lost weight during the initial exercise period because of greater energy expenditure. The effect of exercise was temporary, and the mice gained weight after the second week. Several serum proteins were found to be altered by exercise: haptoglobin was decreased by exercise in Cpefat/fat mice, and several kallikreins were increased by exercise in wild-type mice.


CONCLUSIONS
The access to exercise wheels provided an initial weight loss in Cpefat/fat mice, but this effect was offset by elevated food consumption. The serum proteomics results indicated that Cpefat/fat and wild-type mice differed in their response to exercise.
Yuan
Quan
Q
Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Fontenele-Neto
Jose D
JD
Fricker
Lloyd D
LD
D000273:Adipose Tissue; D000375:Aging; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D001823:Body Composition; D001835:Body Weight; D043423:Carboxypeptidase H / Q000235:genetics* / Q000502:physiology; D004435:Eating; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D009765:Obesity / Q000097:blood* / Q000235:genetics*; D005082:Physical Exertion / Q000502:physiology*; D017354:Point Mutation
D001786:Blood Glucose; D001798:Blood Proteins; D043423:Carboxypeptidase H