Detection of Helicobacter pylori in water by direct PCR.
OBJECTIVE
This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR.


RESULTS
Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample.


CONCLUSIONS
DPCR should provide an improved method to assess contamination of water by H. pylori.


CONCLUSIONS
This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector.
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA.
J A
JA
Benson
K A
KA
Fode-Vaughan
M L P
ML
Collins
D004269:DNA, Bacterial / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D004269:DNA, Bacterial