Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis.
OBJECTIVE
To evaluate the effect of oxidative stress on the spermatogenesis and lactate dehydrogenase-X (LDH-X) activity in mouse testis.


METHODS
For creating different levels of oxidative stress in mice, three selenium (Se) level diets were fed in separate groups for 8 weeks. Group 1 animals were fed yeast-based Se-deficient (0.02 ppm) diet. Group 2 and Group 3 animals were fed with the same diet supplemented with 0.2 ppm and 1 ppm Se as sodium selenite, respectively. After 8 weeks, biochemical and histopathological observations of the testis were carried out. LDH-X levels in the testis were analyzed by western immunoblot and ELISA.


RESULTS
A significant decrease in testis Se level was observed in Group 1 animals, whereas it was enhanced in Group 3 as compared to Group 2. The glutathione peroxidase (GSH-Px) activity was significantly reduced in both the liver and testis in Group 1, but not in Group 2 and 3. A significant increase in the testis glutathione-S-transferase (GST) activity was observed in Group 1, whereas no significant change was seen in Groups 2 and 3. Histological analysis of testis revealed a normal structure in Group 2. A significant decrease in the germ cell population in Group 1 was observed as compared to Group 2 with the spermatids and mature sperm affected the most. Decrease in the lumen size was also observed. In the Se-excess group (Group 3), displacement of germ cell population was observed. Further, a decrease in the LDH-X level in testis was observed in Group 1.


CONCLUSIONS
Excessive oxidative stress in the Se deficient group, as indicated by changes in the GSH-Px/GST activity, affects the spermatogenic process with a reduction in mature sperm and in turn the LDH-X level.
Kaur
Parminder
P
Department of Biophysics, Panjab University, Chandigarh-160014, India.
Bansal
M P
MP
D000818:Animals; D004032:Diet; D005982:Glutathione Transferase / Q000378:metabolism; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D012643:Selenium / Q000172:deficiency* / Q000493:pharmacokinetics / Q000494:pharmacology*; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000473:pathology / Q000502:physiology*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4; D005982:Glutathione Transferase; D012643:Selenium