Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component.
One DNA A (KA30) and five different DNA B components (KA21, KA22, KA27, KA28 and KA34) of a geminivirus, Mungbean yellow mosaic virus-Vigna (MYMV-Vig) were cloned from a pooled sample of field-infected Vigna mungo plants from Vamban, South India. MYMV-Vig DNA A (KA30) and one of the DNA B components (KA27) exhibited 97% and 95% sequence identities, respectively, to those of MYMV reported from Thailand. However, the DNA B components KA21, KA22, KA28 and KA34 exhibited only 71 to 72% sequence identity to MYMV DNA B. Co-existence of multiple DNA B components in field-infected V. mungo was proved by Southern and PCR analyses. Each of the five DNA B components was infective together with the DNA A upon agroinoculation. Agroinoculation with mixed cultures of Agrobacterium with partial dimers of DNA A and all five DNA Bs proved that all five DNA B components can co-infect a single V. mungo plant.
Centre for Plant Molecular Biology and Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
A S
AS
Karthikeyan
R
R
Vanitharani
V
V
Balaji
S
S
Anuradha
P
P
Thillaichidambaram
P V
PV
Shivaprasad
C
C
Parameswari
V
V
Balamani
M
M
Saminathan
K
K
Veluthambi
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis*; D007887:Fabaceae / Q000821:virology*; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity; D007194:India; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence
D004279:DNA, Viral