Electrochemical detection of ascorbic acid (vitamin C) using a glassy carbon electrode.
A method for the detection of ascorbic acid (AA) was developed using square wave anodic stripping voltammetric (SWASV) analysis with a glassy carbon electrode (GCE). The results indicated a sensitive oxidation peak current of AA on the GCE. A linear curve was obtained within a concentration range of 1-130.0 microg/L with a preconcentration time of 270 s. The relative standard deviation of 30.0 microg/L observed was 0.1016% (n = 12) under optimum conditions. The low detection limit (S/N) was pegged at 0.30 microg/L. Results showed that the method developed can be used to assay biological and pharmaceutical samples, and food samples, as well as other materials requiring AA analyses.
Ly
Suw Young
SY
Department of Fine Chemistry, School of Applied Chemical Engineering, Seoul National University of Technology, Seoul, South Korea. suwyoung@snut.ac.kr
Chae
Jung Ik
JI
Jung
Young Sam
YS
Jung
Woon Won
WW
Lee
Hye Jeong
HJ
Lee
Seong Ho
SH
D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000975:Antioxidants; D001205:Ascorbic Acid