Analysis of human chorionic gonadotropin-monoclonal antibody interaction in BIAcore.
Kinetic studies of macromolecular ligant-ligate interaction have generated ample interest since the advent of plasmon resonance based instruments like BIAcore. Most of the studies reported in literature assume a simple 1:1 Langmuir binding and complete reversibility of the system. However we observed that in a high affinity antigen-antibody system [human chlorionic gonadotropin-monoclonal antibody (hCG-mAb)] dissociation in insignificant and the sensogram data cannot be used to measure the equilibrium and kinetic parameters. At low concentrations of mAb the complete sensogram could be fitted to a single exponential. Interestingly we found that at higher mAb concentrations, the binding data did not conform to a sample biomolecular model. Instead, the data fitted a two-step model, which may be because of surface heterogeneity of affinity sites. In this paper, we report on the global fit of the sensograms. We have developed a method by which a single two-minute sensogram can be used in high affinity systems to measure the association rate constant of the reaction and the functional capacity of the ligand (hCG) immobilized on the chip. We provide a rational explanation for the discrepancies generally observed in most of the BIAcore sensograms.
Ashish
Banerjee
B
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.
Murthy
Gundlupet Satyanarayana
GS
D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001665:Binding Sites; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D008024:Ligands