Growth behavior in plant cell cultures based on emissions detected by a multisensor array.
The use of a multisensor array based on chemical gas sensors to monitor plant cell cultures is described. The multisensor array, also referred to as an electronic nose, consisted of 19 different metal oxide semiconductor sensors and one carbon dioxide sensor. The device was used to continuously monitor the off-gas from two plant cell suspension cultures, Morinda citrifolia and Nicotiana tabacum, cultivated under batch conditions. By analyzing the multiarray responses using two pattern recognition methods, principal component analysis and artificial neural networks, it was possible to monitor the course of the cultivations and, in turn, to predict (1) the biomass concentration in both systems and (2) the formation of the secondary metabolite, antraquinone, by M. citrifolia. The results identify the multisensor array method as a potentially useful analytical tool for monitoring plant process variables that are otherwise difficult to analyze on-line.
Komaraiah
Palle
P
Division of Biotechnology, Linköping University, I.F.M., SE-581 83 Linköping, Sweden.
Navratil
Marian
M
Carlsson
Maria
M
Jeffers
Paul
P
Brodelius
Maria
M
Brodelius
Peter E
PE
Kieran
Patricia M
PM
Mandenius
Carl-Fredrik
CF
D015374:Biosensing Techniques; D002455:Cell Division; D002460:Cell Line; D032066:Morinda / Q000166:cytology*; D016571:Neural Networks, Computer; D014026:Nicotiana / Q000166:cytology*