Production of recombinant proteins: challenges and solutions.
Efficient strategies for the production of recombinant proteins are gaining increasing importance, as more applications that require high amounts of high-quality proteins reach the market. Higher production efficiencies and, consequently, lower costs of the final product are needed for obtaining a commercially viable process. In this chapter, common problems in recombinant protein production are reviewed and strategies for their solution are discussed. Such strategies include molecular biology techniques, as well as manipulation of the culture environment. Finally, specific problems relevant to different hosts are discussed.
Instituto de Biotechnología, UNAM, Cuernavaca, Morelos, México.
Laura A
LA
Palomares
Sandino
S
Estrada-Mondaca
Octavio T
OT
Ramírez
D019149:Bioreactors; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006031:Glycosylation; D010957:Plasmids; D014176:Protein Biosynthesis / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D016662:Virus Integration; D014780:Viruses; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D021382:Protein Sorting Signals; D011506:Proteins