Expression of cytochrome P450-4A isoforms in the rat cremaster muscle microcirculation.
OBJECTIVE
This study sought to identify any specific cytochrome P450 (CYP450) -4A enzyme isoforms expressed in arterioles and/or the surrounding parenchymal tissue of the rat cremaster muscle.


METHODS
RT-PCR was used to detect the presence of specific CYP450-4A isoforms in isolated muscle fibers and arterioles from the cremaster muscle of Sprague-Dawley rats; CYP450-4A protein expression was determined by Western blotting.


RESULTS
CYP450-4A3 mRNA was expressed in isolated muscle fibers and in cremasteric arterioles, while CYP450-4A8 mRNA was expressed only in cremasteric arterioles. CYP450-4A1 and CYP450-4A2 mRNA were not expressed in arterioles and skeletal muscle cells, although all four isoforms were strongly expressed in the liver. CYP450-4A protein was detected in both the isolated muscle fibers and in the isolated arterioles.


CONCLUSIONS
The present study identifies the specific pattern of cytochrome P450-4A isoform expression in arterioles and parenchymal cells of the skeletal muscle microcirculation, and supports the hypothesis that the cytochrome P-450 enzymes may play a role in the regulation of microvascular function in the skeletal muscle microcirculation.
Wang
Jingli
J
Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Maier
Kristopher G
KG
Roman
Richard J
RJ
De La Cruz
Lourdes
L
Zhu
Jiaxuan
J
Henderson
Lisa
L
Lombard
Julian H
JH
D000818:Animals; D001160:Arterioles / Q000201:enzymology*; D042926:Cytochrome P-450 CYP4A / Q000032:analysis / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000235:genetics*; D000072470:Cytochrome P450 Family 4; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D008297:Male; D008833:Microcirculation / Q000201:enzymology; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000166:cytology / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
C465387:Cyp4a1protein, rat; D007527:Isoenzymes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D000072470:Cytochrome P450 Family 4; C465389:Cyp4a3 protein, rat; D042926:Cytochrome P-450 CYP4A; C465388:cytochrome P-450 CYP4A2 (rat)