Production of 2-hydroxy-4-methoxybenzaldehyde in roots of tissue culture raised and acclimatized plants of Decalepis hamiltonii Wight & Arn., an endangered shrub endemic to Southern India and evaluation of its performance vis-a-vis plants from natural habitat.
Axillary buds obtained from field grown plants of D. hamiltonii were used to initiate multiple shoots on Murashige and Skoog's medium (MS) supplemented with 2 mg L(-1) 6-benzyl aminopurine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Profuse rooting was achieved when the actively growing shoots were cultured on MS medium supplemented with 1.0 mg l(-1) indole-3-butyric acid (IBA). Regenerated plants were grown successfully in the plains, in contrast to wild growth in high altitudes and rocky crevices of hilly regions. Roots of different sizes from one-year-old tissue culture raised field grown plants had the same profile of 2-hydroxy-4-methoxybenzaldehyde as that of wild plants. A maximum of 0.14% and 0.12% 2-hydroxy-4-methoxybenzaldehyde was produced in roots of one year old tissue culture derived plants and greenhouse grown plants respectively.
Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 013, India.
P
P
Giridhar
T
T
Rajasekaran
S
S
Nagarajan
G A
GA
Ravishankar
D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D002455:Cell Division; D046508:Culture Techniques; D008401:Gas Chromatography-Mass Spectrometry; D007194:India; D007210:Indoleacetic Acids / Q000378:metabolism; D008956:Models, Chemical; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D013696:Temperature
D001547:Benzaldehydes; D007210:Indoleacetic Acids; C118297:2-hydroxy-4-methoxybenzaldehyde; C030737:indoleacetic acid