[Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing].
Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, 660036 Russia.
T G
TG
Volova
N A
NA
Voĭnov
D002248:Carbon Monoxide; D003031:Coal / Q000382:microbiology*; D003470:Culture Media; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005740:Gases / Q000378:metabolism; D011091:Polyesters / Q000032:analysis / Q000378:metabolism*; D013379:Substrate Specificity
D003031:Coal; D003470:Culture Media; D005740:Gases; D011091:Polyesters; D002248:Carbon Monoxide