The regulation of human beta-defensin 2 by the ETS transcription factor MEF (myeloid Elf-1-like factor) is enhanced by promyelocytic leukemia protein.
The human beta-defensin 2 (HBD 2) is a potent anti-bacterial peptide with a wide spectrum of activity. MEF (myeloid elf-1-like factor) or Elf4, a member of the ETS transcription factor family, has been shown to up-regulate the basal expression of the HBD 2 gene in epithelial cells. The mammalian cell nucleus is organized into distinct nuclear domains, one of which is the PML (promyelocytic leukemia) nuclear body involved in the regulation of transcription. Here, we show that PML stimulated MEF transcriptional activity, resulting in the up-regulation of endogenous HBD 2 expression.
Suico
Mary Ann
MA
Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan.
Lu
Zhuo
Z
Shuto
Tsuyoshi
T
Koga
Tomoaki
T
Uchikawa
Tomoko
T
Yoshida
Hiroki
H
Matsuzaki
Kazuhito
K
Nakao
Mitsuyoshi
M
Li
Jian-Dong
JD
Kai
Hirofumi
H
D000890:Anti-Infective Agents / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000502:physiology*; D009687:Nuclear Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D000071156:Promyelocytic Leukemia Protein; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D004268:DNA-Binding Proteins; C111931:ELF4 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D023083:beta-Defensins; C070576:PML protein, human