[Conversion of L-cystine and L-cysteine to taurin by the enzyme systems of liver cells].
The kinetics of conversion of sulfur-containing amino acids L-cystine and L-cysteine to taurin by the enzyme system of cattle liver cells was studied, and a mathematical model was developed. It was shown that L-cystine and L-cysteine conversion obeyed the Michaelis-Menten equations of serial-sequential conversions with regard to inhibition by the final product and inactivation. The yield of taurin under the optimized conditions of L-cystine and L-cysteine conversion (temperature, 40 degrees C; pH 1.5 and 3.0, respectively; and addition of enzyme preparations in five equal portions at 2-h intervals) was in the range 80-85% of the substrate weight.
Mendeleev University of Chemical Technology of Russia, Moscow, 125047 Russia.
A V
AV
Soboleva
A A
AA
Krasnoshtanova
I A
IA
Krylov
D000818:Animals; D002417:Cattle; D003545:Cysteine / Q000378:metabolism*; D003553:Cystine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008962:Models, Theoretical; D013654:Taurine / Q000378:metabolism*; D013696:Temperature
D013654:Taurine; D003553:Cystine; D003545:Cysteine