The 980-nm diode laser for brain surgery: histopathology and recovery period.
The 980-nm diode laser has been under investigation for neurosurgery because of a local peak in the absorption spectra of water around this wavelength. This work was carried out to examine the extent of thermal changes and the recovery process of laser-induced brain lesions. In order to study the quality of the lesions, a conventional monopolar electrocoagulation technique was applied comparatively. An in vivo stereotaxic neurosurgical procedure was performed on Wistar rats. Bilateral brain lesions of the same size (2-3 mm diameter) were created with a diode laser and via electrocoagulation. Subjects were sacrificed 0, 2 and 7 days after surgery in order to observe the healing process of the necrotic tissue. The surgical after-effects of both types of lesions were identified through immunohistochemical staining with CD68 macrophage marker and haematoxylin eosin (H&E). CD68 was found to be more efficient than H&E in determining the thermally altered areas. Histological examinations showed that the 980-nm diode laser system has a remarkable ablating ability with minimal thermal damage of nearby tissue.
Institute of Biomedical Engineering, Bogazici University, Bebek, Istanbul, Turkey.
Ozguncem
O
Bozkulak
Hasim Ozgur
HO
Tabakoglu
Ayla
A
Aksoy
Ozlem
O
Kurtkaya
Aydin
A
Sav
Resit
R
Canbeyli
Murat
M
Gulsoy
D000818:Animals; D001921:Brain / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D004564:Electrocoagulation; D005260:Female; D007150:Immunohistochemistry; D053685:Laser Therapy; D008028:Light Coagulation; D008264:Macrophages / Q000473:pathology; D017628:Microglia / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar