High gene expression of the mutant adenovirus vector, both in vitro and in vivo, with the insertion of integrin-targeting peptide into the fiber.
In the present study, a first-generation adenovirus (Ad) vector was modified with the RGD peptide inserted into the fiber. The insertion of an integrin-targeting sequence into the Ad vector notably enhanced the luciferase expression in the Coxsackie virus and Adenovirus Receptor-deficient A2058 and B16BL6 melanoma cells. The results of an in vivo study with tumor-bearing mice also showed that Ad-RGD-Luc had enhanced gene expression in many organs and in the B16BL6 tumor compared to that induced by a conventional Ad vector after intravenous injection.
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Japan.
J Q
JQ
Gao
S
S
Inoue
Y
Y
Tsukada
K
K
Katayama
Y
Y
Eto
S
S
Kurachi
H
H
Mizuguchi
T
T
Hayakawa
Y
Y
Tsutsumi
T
T
Mayumi
S
S
Nakagawa
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D016023:Integrins / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008546:Melanoma, Experimental / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics*; D009842:Oligopeptides / Q000096:biosynthesis / Q000235:genetics; D010455:Peptides / Q000235:genetics*
D004251:DNA Transposable Elements; D016023:Integrins; D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid; D008156:Luciferases