Identification of collagen encapsulation at the dentin/adhesive interface.
OBJECTIVE
The purpose of this study was to investigate the dentin/adhesive interfacial characteristics of three current commercial adhesives with different relative hydrophilic/hydrophobic composition, using a nondestructive staining technique.


METHODS
Dentin surfaces of 18 unerupted human third molars were randomly selected for treatment with one of three commercial dentin bonding agents according to manufacturers' instructions for the "wet" bonding technique. The adhesives were ranked based on hydrophilic/hydrophobic component ratios (ie, ability to dissolve in water), highest to lowest, as follows: Uno (Pulpdent) > Prime&Bond NT (PBNT, Dentsply Caulk) > Single Bond (SB, 3M ESPE). Dentin/adhesive (d/a) interface sections were stained with Goldner's trichrome, a classical bone stain, and examined using light microscopy.


RESULTS
The extent and degree to which the adhesive encapsulates the demineralized dentin matrix is reflected in the color differences in the stained sections. The depth of demineralization appeared comparable among these bonding systems, but adhesive infiltration varied from highest to lowest as follows: Uno > PBNT > SB.


CONCLUSIONS
The differential staining technique provided a clear representation of the depth of dentin demineralization and extent/degree of adhesive encapsulation of the exposed collagen at the d/a interface. This technique provides a mechanism for readily identifying vulnerable sites at the d/a interface. The composition of the one-bottle adhesive systems has a substantial effect on the interfacial structure of the d/a bond.
Department of Oral Biology, University of Missouri-Kansas City, School of Dentistry, Kansas City, MO 64108-2784, USA. spencerp@umkc.edu
Paulette
P
Spencer
Yong
Y
Wang
J Lawrence
JL
Katz
D000134:Acid Etching, Dental; D001391:Azo Compounds; D001565:Benzoates / Q000737:chemistry; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003094:Collagen / Q000648:ultrastructure*; D004396:Coloring Agents; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004801:Eosine Yellowish-(YS); D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry; D008739:Methyl Green; D010756:Phosphoric Acids / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012995:Solubility; D014867:Water / Q000737:chemistry
D001391:Azo Compounds; D001565:Benzoates; D004396:Coloring Agents; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D010756:Phosphoric Acids; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C115612:single bond; C017768:trichrome stain; D014867:Water; C080129:pyromellitic dianhydride glycerol dimethacrylate adduct; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; D008739:Methyl Green; D003094:Collagen; D004801:Eosine Yellowish-(YS)