The ab initio crystal structure solution of proteins by direct methods. VI. Complete phasing up to derivative resolution.
The procedure described in the papers I-V of this series [Giacovazzo, Siliqi & Ralph (1994). Acta Cryst. A50, 503-505; Giacovazzo, Siliqi & Spagna (1994). Acta Cryst. A50, 609-621; Giacovazzo, Siliqi & Zanotti (1995). Acta Cryst. A51, 177-188; Giacovazzo & Gonzalez Platas (1995). Acta Cryst. A51, 398-404; Giacovazzo, Siliqi & Gonzalez Platas (1995). Acta Cryst. A51, 811-820], aiming at estimating protein phases via a single heavy-atom derivative, has been improved so as to extend phase determination to all the reflections up to derivative resolution. The quality of the resulting electron-density maps is checked for a number of test strutures. Some of the maps are immediately interpretable, and some can be interpreted after some cycles of solvent flattening and/or histogram matching. The correlation with classical SIR techniques is also discussed.
Giacovazzo
C
C
Dipartimento Geomineralogico, Universita' di Bari, Italy. giacovazzo@area.ba.cnr.it
Siliqi
D
D
Gonzalez Platas
J
J
Hecht
H J
HJ
Zanotti
G
G
York
B
B