Direct evidence of the existence of specific LPS binding sites on vascular endothelial cells.
OBJECTIVE
To investigate the binding characteristics of endothelial cell (EC) with LPS free from the participation of serum factors.


METHODS
Laser confocal microscope was employed in the observation of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [(3)H]-LPS.


RESULTS
The binding of EC with LPS was saturable, time and concentration dependent and it could be competed with overdosed LPS of the same type. The fluorescence mainly distributed in cytoplasm, especially near the nucleus, which could also be stained.


CONCLUSIONS
There might be some specific LPS binding sites existing on ECs and LPS could function intracellularily.
Chen
Jian
J
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. hhhcc@tmmu.com.cn
Luo
Xiang-dong
XD
Luo
Qin
Q
Yin
Jun-fei
JF
Fu
Guo-jin
GJ
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D018613:Microscopy, Confocal; D011869:Radioligand Assay; D014471:Umbilical Veins / Q000166:cytology
D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; D016650:Fluorescein-5-isothiocyanate