Dendrimer-functionalized self-assembled monolayers as a surface plasmon resonance sensor surface.
We report here a multistep route for the immobilization of DNA and proteins on chemically modified gold substrates using fourth-generation NH(2)-terminated poly(amidoamine) dendrimers supported by an underlying amino undecanethiol (AUT) self-assembled monolayer (SAM). Bioactive ultrathin organic films were prepared via layer-by-layer self-assembly methods and characterized by fluorescence microscopy, variable angle spectroscopic ellipsometry, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and attenuated total internal reflection Fourier transform infrared spectroscopy (ATR-FTIR). The thickness of the AUT SAM base layer on the gold substrates was determined to be 1.3 nm from ellipsometry. Fluorescence microscopy and AFM measurements, in combination with analyses of the XPS/ATR-FTIR spectra, confirmed the presence of the dendrimer/biopolymer molecules on the multilayer sensor surfaces. Model proteins, including streptavidin and rabbit immunoglobulin proteins, were covalently attached to the dendrimer layer using linear cross-linking reagents. Through surface plasmon resonance measurements, we found that sensor surfaces containing a dendrimer layer displayed an increased protein immobilization capacity, compared to AUT SAM sensor surfaces without dendrimer molecules. Other SPR studies also revealed that the dendrimer-based surfaces are useful for the sensitive and specific detection of DNA-DNA interactions. Significantly, the multicomponent films displayed a high level of stability during repeated regeneration and hybridization cycles, and the kinetics of the DNA-DNA hybridization process did not appear to be influenced by surface mass transport limiting effects.
Mark
Sonny S
SS
Department of Microbiology, Cornell University, Ithaca, New York 14853. USA. ssm12@cornell.edu
Sandhyarani
Neelakantapillai
N
Zhu
Changcheng
C
Campagnolo
Christine
C
Batt
Carl A
CA
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D007103:Immobilization; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D011073:Polyamines / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
D050091:Dendrimers; D008567:Membranes, Artificial; D011073:Polyamines; D011506:Proteins; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA