Refined three-dimensional structure of cat-muscle (M1) pyruvate kinase at a resolution of 2.6 A.
The three-dimensional structure of cat-muscle pyoruvate kinase has been refined at a resolution of 2.6 A. The details of the structure permit interpretation of the original heavy-atom studies and give insight into the importance of conserved residues in pyruvate kinases and the allosteric behaviour of the enzyme. There are a small number of essential residues which determine the relative orientations of domains and the precise nature of intersubunit contacts. Arginine residues are particularly important.
Department of Biochemistry and Molecular Recognition Centre, School of Medical Sciences, University of Bristol.
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Allen
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Muirhead