Improvement of anemia associated with chronic renal failure by recombinant human erythropoietin treatment in ICR-derived glomerulonephritis (ICGN) mice.
The ICR-derived glomerulonephritis (ICGN) mouse, a novel inbred mouse strain with a hereditary nephrotic syndrome, develops severe anemia associated with chronic renal failure. To reveal the pathogenic mechanism of anemia in ICGN mice, we subcutaneously administered recombinant human erythropoietin (rhEPO; 5 IU/mouse/day) or saline for 5 days to ICGN mice. In terminal-stage ICGN mice with severe anemia, rhEPO significantly increased hematocrit (Ht), red blood cells (RBC) and hemoglobin levels. Endogenous EPO levels in peripheral blood were reduced by rhEPO injection. No histopathological changes in bone marrow and kidneys were induced by rhEPO injection. Insufficiency of EPO may cause anemia in ICGN mice.
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Japan.
Misuzu
M
Yamaguchi-Yamada
Noboru
N
Manabe
Yasufumi
Y
Goto
Sayuri
S
Anan
Keiko
K
Miyamoto
Youhei
Y
Miyamoto
Masaya
M
Nagao
Yoshie
Y
Yamamoto
Atsuo
A
Ogura
D000740:Anemia / Q000188:drug therapy* / Q000209:etiology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D004195:Disease Models, Animal; D004906:Erythrocyte Count; D004921:Erythropoietin / Q000627:therapeutic use*; D005260:Female; D005921:Glomerulonephritis / Q000150:complications / Q000188:drug therapy*; D006400:Hematocrit; D006454:Hemoglobins; D007279:Injections, Subcutaneous; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011994:Recombinant Proteins
D006454:Hemoglobins; D011994:Recombinant Proteins; D004921:Erythropoietin