Prokaryotic expression of antibodies and affibodies.
Recent advances have been made in the development of systems for the display and expression of recombinant antibodies and affibodies in filamentous phages, Escherichia coli and other prokaryotic cells. Emphasis has been placed on improving phage and phagemid vectors, alternative systems for expression in different cellular compartments (e.g. the outer membrane, periplasm, cytoplasm and extracellular secretion) and novel multimerization systems for generating bivalent or multivalent binding molecules.
Fernández
Luis Angel
LA
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain. lafdez@cnb.uam.es
D000906:Antibodies / Q000235:genetics; D000917:Antibody Formation / Q000502:physiology*; D000941:Antigens / Q000096:biosynthesis* / Q000235:genetics; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D019151:Peptide Library; D011387:Prokaryotic Cells / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*
D000906:Antibodies; D000941:Antigens; D019151:Peptide Library