Disrupted expression of intermediate filaments in the testis of rhesus monkey after experimental cryptorchidism.
Cytoskeletons in Sertoli cell play an important role in process of spermatogenesis. The expression and distribution of the intermediate filaments, vimentin, keratin and desmin, were studied in the Sertoli cells of the cryptorchid testis of rhesus monkey. Vimentin was localized in the perinuclear region of Sertoli cells of the normal testis. An intense increase in vimentin immunoreactivity was observed with appearance of disorganized staining in the Sertoli cells of the cryptorchid testes. Cytokeratin 18, a marker of immature Sertoli cells, re-expressed in the cells of the adult cryptorchid testes. Desmin was also observed in the Sertoli cells in addition to the peritubular myoid cells on 30 days after the cryptorchid operation. These data suggest that Sertoli cells in primate can be affected by the heat stress. The altered changes in intermediate filaments could be possible to induce the Sertoli cell functional changes that would partially contribute to the germ cell apoptosis leading to azoospermia or oligozoospermia.
Zhang
Zhi-Hong
ZH
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China.
Hu
Zhao-Yuan
ZY
Song
Xin-Xin
XX
Xiao
Li-Juan
LJ
Zou
Ru-Jin
RJ
Han
Chun-Sheng
CS
Liu
Yi-Xun
YX
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D013737:Testis / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
D014746:Vimentin; D007633:Keratins