A synthetic peptide from a heptad repeat region of herpesvirus glycoprotein B inhibits virus replication.
Glycoprotein B (gB) is the most conserved glycoprotein of herpesviruses and plays important roles in virus infectivity. Two intervening heptad repeat (HR) sequences were found in the C-terminal half of all herpesvirus gBs analysed. A synthetic peptide derived from the HR region (aa 477-510) of bovine herpesvirus type 1 (BoHV-1) gB was studied for its ability to inhibit virus replication. The peptide interfered with cell-to-cell spread and consistently inhibited replication of BoHV-1, with a 50 % effective concentration value (EC(50)) of 5 microM. Inhibition of replication was obtained not only with herpesviruses including pseudorabies virus and herpes simplex virus type 1 but also partly with Newcastle disease virus. Possible mechanisms of membrane fusion inhibition by the peptide are discussed.
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Katsunori
K
Okazaki
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Hiroshi
H
Kida
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D020449:Repetitive Sequences, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000494:pharmacology*; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects*
D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins