Detection of the impairment of CD80 expression on circulating monocytes in HIV-infected Thai children.
The mechanism of progressive anergic response in HIV-infected children has yet to be adequately described. One possibility is inappropriate delivery of an essential second signal for T-cell activation due to the inappropriate presentation of co-stimulatory molecules. To determine whether the ligand for the secondary signal is impaired in pediatric AIDS, we compared the level of CD80 expression by circulating monocytes in HIV-infected and-noninfected children (15 mild/asymptomatic, 13 symptomatic and 12 HIV seronegative children). By two-color flow cytometry analysis, there was no statistically significant difference in the percentage of monocytes expressing CD80 among the groups (i.e., 63.2 +/- 15.8, 60.9 +/- 12.7, 61.04 +/- 10.9 for uninfected children, mild-asymptomatic children and symptomatic children, respectively). However, both infected groups showed statistically significant lower levels of CD80 expression, with mean fluorescent intensities of 40.9 +/- 15.9 and 38.8 +/- 10.7 compared to 57.05 +/- 16.3 for the uninfected control group. Our data demonstrated a correlation between HIV infection and impairment of CD80 by circulating monocytes. Whether the impairment on CD80 expression contributes to destruction of the immunological network in HIV-infected children requires further investigation.
Ubol
Sukathida
S
Department of Microbiology, Faculty of Science, Mahidol University, 272 RAMA VI Rd, Ratchathewee, Bangkok 10400, Thailand. scsul@mahidol.ac.th
Aiamkitsumrit
Benjamaz
B
Nitayaphan
Sorachai
S
Sirinavin
Sayomporn
S
Kitthawee
Sangvorn
S
D018122:B7-1 Antigen / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000472:pathogenicity; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000473:pathology*; D013785:Thailand
D018122:B7-1 Antigen