RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes.
Lymphocyte function-associated antigen 1 (LFA-1) is relatively nonadhesive on resting lymphocytes; however, the mechanisms underlying changes in its adhesiveness are poorly understood. In this study, we generated a Jurkat T cell clone, J+hi1.14, that contained low amounts of mRNA for RhoH, a leukocyte-specific inhibitory Rho family member. J+hi1.14 cells expressed constitutively adhesive LFA-1 and the cells bound spontaneously to intracellular adhesion molecules 1, 2 and 3. Reconstitution of RhoH mRNA expression in J+hi1.14 cells reverted the adhesion phenotype to that of wild-type. We obtained similar results using RNA interference in peripheral blood lymphocytes. These data demonstrate that RhoH is required for maintenance of lymphocyte LFA-1 in a nonadhesive state.
Cherry
Lisa K
LK
Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham & Women's Hospital, Smith Building room 650, 1 Jimmy Fund Way, Boston, Massachusetts 02115, USA.
Li
Xiaoyu
X
Schwab
Pascale
P
Lim
Bing
B
Klickstein
Lloyd B
LB
D015139:Blotting, Southern; D002448:Cell Adhesion / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism; D019169:Jurkat Cells; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D009154:Mutation; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000276:immunology*
D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; C447841:RhoH protein, human; D014157:Transcription Factors; D018799:Intercellular Adhesion Molecule-1; D020741:rho GTP-Binding Proteins