Production of lupin acid phosphatase in transgenic rice for use as a phytate-hydrolyzing enzyme in animal feed.
The acid phosphatase gene from lupin was expressed in transgenic rice plants under the control of the maize ubiquitin promoter or rice chlorophyll a/b binding protein (Cab) promoter. Transgenic rice leaves exhibited up to an 18-fold increase in phytate-hydrolyzing activity. Based on the phytate-hydrolyzing activity at pH 5.5, more than 85% this activity was retained after heat-treatment at 80 degrees C for 15 min, and the heterologous enzyme in leaf sections and leaf extracts was relatively stable during storage. A distinct increase in released phosphate was observed when the heterologous enzyme was mixed with the feed extract. These results suggest that the heterologous enzyme in rice plants may maintain its desired characteristics as a phytate-hydrolyzing enzyme when added to animal feed.
Hamada
Akira
A
Functional Chemicals Laboratory, Mitsui Chemicals, Inc, Togo 1144, Mobara 297-0017, Japan.
Yamaguchi
Ken-Ichi
K
Harada
Michiko
M
Nikumaru
Seiya
S
Wasaki
Jun
J
Shinano
Takuro
T
Matsui
Hirokazu
H
Osaki
Mitsuru
M
Honda
Hideo
H
D000135:Acid Phosphatase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000821:Animal Feed; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015151:Immunoblotting; D031297:Lupinus; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010833:Phytic Acid / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D018744:DNA, Plant; D011506:Proteins; D010833:Phytic Acid; D000135:Acid Phosphatase