Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation.
A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.
Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama, Japan.
Nao
N
Suzuki
Yoshio
Y
Nakano
Akihiro
A
Yoshida
Yoshihisa
Y
Yamashita
Yusuke
Y
Kiyoura
D000190:Actinomyces / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D018441:Biofilms; D003199:Computer Systems; D017931:DNA Primers; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D017931:DNA Primers; D015345:Oligonucleotide Probes