Comparison of the shape parameters of DNA-cationic lipid complexes and model polyelectrolyte-lipid complexes.
In this study, we used a rheological method to study the shape of DNA-cationic lipid complexes and model polyelectrolyte-lipid complexes. We introduced two kinds of anionic polyelectrolytes, sodium polygalacturonate (PGU) and sodium dextran sulfate (DSS), of varying size, as models for DNA. The prepared complexes were incubated under laminar flow conditions. The results show the same quantitative relation between the shape parameter of lipoplexes and the length of anionic polyelectrolytes, including DNA. The rheological behavior of PGU and DSS were similar to that of DNA.
Dalian Institute of Chemical Physics, Chinese Academy of Science, 457 Zhongshan Road, Dalian 116023, China. sunyan@dicp.ac.cn
Yan
Y
Sun
Xingju
X
Yu
Yasuyuki
Y
Takaoka
Sho-ichi
S
Oh-i
Nejat
N
Duzguneş
Sadao
S
Hirota
D000838:Anions; D002412:Cations; D004247:DNA / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D012212:Rheology
D000838:Anions; D002412:Cations; D008055:Lipids; D008081:Liposomes; D011108:Polymers; D004247:DNA