Metabolomic analysis of Strychnos nux-vomica, Strychnos icaja and Strychnos ignatii extracts by 1H nuclear magnetic resonance spectrometry and multivariate analysis techniques.
1H Nuclear magnetic resonance spectrometry and multivariate analysis techniques were applied for the metabolic profiling of three Strychnos species: Strychnos nux-vomica (seeds, stem bark, root bark), Strychnos ignatii (seeds), and Strychnos icaja (leaves, stem bark, root bark, collar bark). The principal component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between all samples, using the three first components. The key compounds responsible for the discrimination were brucine, loganin, fatty acids, and Strychnos icaja alkaloids such as icajine and sungucine. The method was then applied to the classification of several "false angostura" samples. These samples were, as expected, identified as S. nux-vomica by PCA, but could not be clearly discriminated as root bark or stem bark samples after further statistical analysis.
Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, P.O. Box 9502, NL-2300 RA, Leiden, The Netherlands.
Michel
M
Frédérich
Young Hae
YH
Choi
Luc
L
Angenot
Goetz
G
Harnischfeger
Alfons W M
AW
Lefeber
Robert
R
Verpoorte
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D015999:Multivariate Analysis; D010936:Plant Extracts / Q000032:analysis*; D031451:Strychnos / Q000737:chemistry* / Q000145:classification
D010936:Plant Extracts