A comparison of topical formulations for the prevention of human schistosomiasis.
Recently, a dimeticone formulation has been shown to be effective at preventing Schistosoma cercariae infecting skin, while DEET (N,N-diethyl-m-toluamide), a highly effective insecticide, has been shown to have activity against cercariae. Seven formulations, 3 containing DEET, were prepared and applied to excised human skin in Franz cells for 1 h. Schistosoma cercariae were applied for 30 min at 1 and 24 h, and the number that penetrated the skin calculated (n = 9). DEET could not be incorporated into the dimeticone formulation, yet it remained the most effective at preventing cercarial penetration, both 1 and 24 h after application. The ointments that contained DEET did prevent penetration but their mode of action was due to the toxicity of DEET against the cercariae. The persistence of the protection afforded by the dimeticone formulation after washing suggests that the formulation may be interacting with the stratum corneum to prevent cercarial recognition of skin.
Cooper
E
E
Department of Pharmacy, King's College London, 150 Stamford St, SE1 9NN, UK.
Iqbal
A
A
Bartlett
A
A
Marriott
C
C
Whitfield
P J
PJ
Brown
M B
MB
D000818:Animals; D002626:Chemistry, Pharmaceutical; D003671:DEET / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007302:Insect Repellents / Q000008:administration & dosage / Q000627:therapeutic use*; D012550:Schistosoma mansoni / Q000187:drug effects*; D012552:Schistosomiasis / Q000517:prevention & control*; D012867:Skin / Q000187:drug effects
D007302:Insect Repellents; D003671:DEET