Measurement of novel dietary fibers.
With the recognition that resistant starch (RS) and nondigestible oligosaccharides (NDO) act physiologically as dietary fiber (DF), a need has developed for specific and reliable assay procedures for these components. The ability of AOAC DF methods to accurately measure RS is dependent on the nature of the RS being analyzed. In general, NDO are not measured at all by AOAC DF Methods 985.29 or 991.43, the one exception being the high molecular weight fraction of fructo-oligosaccharides. Values obtained for RS, in general, are not in good agreement with values obtained by in vitro procedures that more closely imitate the in vivo situation in the human digestive tract. Consequently, specific methods for the accurate measurement of RS and NDO have been developed and validated through interlaboratory studies. In this paper, modifications to AOAC fructan Method 999.03 to allow accurate measurement of enzymically produced fructo-oligosaccharides are described. Suggested modifications to AOAC DF methods to ensure complete removal of fructan and RS, and to simplify pH adjustment before amyloglucosidase addition, are also described.
Megazyme International Ireland Ltd., Bray Business Park, Southern Cross Rd, Bray, County Wicklow, Ireland. barry@megazyme.com
Barry V
BV
McCleary
Patricia
P
Rossiter
D004043:Dietary Fiber / Q000032:analysis*; D004798:Enzymes / Q000737:chemistry; D005630:Fructans / Q000032:analysis; D006593:Hexokinase / Q000737:chemistry; D007202:Indicators and Reagents; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013213:Starch / Q000032:analysis
D004043:Dietary Fiber; D004798:Enzymes; D005630:Fructans; D007202:Indicators and Reagents; D009844:Oligosaccharides; D011134:Polysaccharides; C008315:maltodextrin; D013213:Starch; D006593:Hexokinase