Alterations in embryo development in progestogen-supplemented cows administered prostaglandin F2alpha.
The objective was to examine effects of elevated prostaglandin F2alpha (PGF) on embryo development in cows supplemented with exogenous progestogen. Cows were artificially inseminated at estrus (Day 0) and a synthetic progestogen supplemented in the feed from Days 3 to 8. Cows were allotted randomly to receive either 15 mg PGF (TRT) or saline (CON) at 06:00, 14:00 and 22:00 h from Days 5 to 8. Blood samples were collected at 06:00 and 22:00 h from Days 5 to 8 for determination of progesterone and 13,14-dihydro-15-keto-PGF2alpha (PGFM). Single embryos were recovered on Day 8, assigned a quality score, and stage of development recorded. Progesterone was lower from Days 5 to 8 in TRT versus CON cows (P = 0.0001). Concentrations of PGFM from Days 5 to 8 were elevated in TRT compared to CON cows (P = 0.0001). Embryo quality was reduced in TRT cows compared to CON cows (P = 0.059). Percentage of embryos considered transferable was decreased by administration of PGF (P = 0.003). Sixty-four percent of TRT embryos were retarded in development at Day 8, whereas 80% of CON embryos had developed to expanded blastocysts (P = 0.003). In conclusion, treatment of progestogen-supplemented cows with PGF reduced quality and retarded development of embryos. Decreased fertility in conditions causing elevated concentrations of PGF may result from altered embryo development and quality.
Hockett
M E
ME
Department of Animal Science, University of Tennessee, 205 C Brehm Animal Science Building, Knoxville, TN 37996-4574, USA.
Rohrbach
N R
NR
Schrick
F N
FN
D000818:Animals; D002417:Cattle; D015237:Dinoprost / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005260:Female; D011374:Progesterone / Q000097:blood; D045167:Progesterone Congeners / Q000494:pharmacology*
D045167:Progesterone Congeners; D011374:Progesterone; D015237:Dinoprost