Characterization of the DNA adenine 5'-GATC-3' methylase HpyIIIM from Helicobacter pylori.
The effect of inactivation of the 5'-GATC-3' methylase HpyIIIM in Helicobacter pylori (H. pylori) on mismatch repair, adherence, and in vitro fitness was examined. Chromosomal DNA from 90 H. pylori strains was isolated, and restriction enzyme digestion indicated all strains examined possess HpyIIIM. Wild-type H. pylori and a strain with an inactive HpyIIIM were found to have rifampicin mutation frequencies of 2.93 x 10(-7) and 1.05 x 10(-7) (p > 0.05), respectively, indicating that HpyIIIM does not appear to be important in mismatch repair. Adherence of H. pylori in an in vitro model cell system was also unaffected by inactivation of HpyIIIM. Inactivation of HpyIIIM did not result in a decrease in fitness, as determined by liquid in vitro competition experiments.
Department of Medical Microbiology and Immunology, University of Alberta, 128 Medical Sciences Building, Edmonton, Alberta, Canada T6G 2H7.
Joanne L
JL
Simala-Grant
Eugene
E
Lam
Monika
M
Keelan
Diane E
DE
Taylor
D001422:Bacterial Adhesion / Q000502:physiology; D002460:Cell Line; D004260:DNA Repair / Q000502:physiology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D015252:Deoxyribonucleases, Type II Site-Specific