Mechanisms underlying diquertin-mediated regulation of neutrophil function in patients with non-insulin-dependent diabetes mellitus.
We studied the effects of dihydroquercetin (3.3.4.5.7-pentahydroxyflavanone, a new Russian patented preparation) on functional activity of polymorphonuclear neutrophils from patients with non-insulin-dependent diabetes mellitus. Flavonoids (quercetin and its derivative dihydroquercetin) dose-dependently suppressed generation of anion radicals and hypochlorous acid and production of malonic dialdehyde during oxidation of neutrophil membranes. Dihydroquercetin decreased activities of protein kinase C and myeloperoxidase in activated polymorphonuclear neutrophils and could bind transition metals (Fe2+). These properties determine the ability of dihydroquercetin to decrease in vitro functional activity of polymorphonuclear neutrophils from patients with non-insulin-dependent diabetes mellitus.
Fedosova
N F
NF
Department of General Pathology and Pathophysiology, Russian Medical Academy of Postgraduate Education, Russian Ministry of Health, Moscow, Russia.
Alisievich
S V
SV
Lyadov
K V
KV
Romanova
E P
EP
Rudko
I A
IA
Kubatiev
A A
AA
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000494:pharmacology*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000503:physiopathology*; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; C473100:diquertin; D013481:Superoxides; D006997:Hypochlorous Acid; D011794:Quercetin