Crystallization and preliminary crystallographic data for the augmenter of liver regeneration.
A new cellular growth factor termed augmenter of liver regeneration (ALR) has been crystallized. ALR has been shown to have a proliferative effect on liver cells while at the same time producing an immunosuppressive effect on liver-resident natural killer cells and liver-resident mononuclear leukocytes. In addition, ALR appears to play an important role in the synthesis and stabilization of mitochondrial gene transcripts in actively regenerating cells. ALR crystals diffract to beyond 2 A resolution and belong to space group P2(1)2(1)2, with a = 125.1, b = 108.1 and c = 38.5 A. Based on four molecules per asymmetric unit, the Matthews coefficient is calculated to be 2.16 A(3) Da(-1) which corresponds to a solvent content of 43%.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA. rose@bcl4.biochem.uga.edu
J P
JP
Rose
C K
CK
Wu
A
A
Francavilla
J G
JG
Prelich
A
A
Iacobellis
M
M
Hagiya
A S
AS
Rao
T E
TE
Starzl
B C
BC
Wang