Chitinase induced by jasmonic acid, methyl jasmonate, ethylene and protein phosphatase inhibitors in rice.
Chitinase is a pathogenesis-related protein that hydrolyzes chitin, a major component of fungal cell walls. Two-week-old rice seedling leaf, leaf sheath and root tissues responded to an exogenous treatment by jasmonic acid (JA) with induction of the chitinases as determined by immunoblot analysis using an anti-endochitinase antibody. Induced accumulation of these chitinases was observed within 24 to 48 h in the leaf sheaths, leaves and roots. Besides, ethylene generator ethephon and abiotic stressor copper could also induce chitinases accumulation among various plant hormones and stress agents examined. Cycloheximide effectively blocked their accumulation by JA, suggesting that de novo protein synthesis is required. Partial blockage of the induced accumulation of chitinases by NADPH oxidase inhibitor and free radical scavengers suggested involvement of reactive oxygen species. Moreover, induced accumulation of these chitinases also by methyl jasmonate and certain protein phosphatase inhibitors indicated their potential importance and wider role in rice seedlings.
Molecular Genetics Department, Institute of Plant Science, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba 305-8602, Japan.
Randeep
R
Rakwal
Guangxiao
G
Yang
Setsuko
S
Komatsu
D000085:Acetates / Q000494:pharmacology; D002688:Chitinases / Q000032:analysis / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D054883:Oxylipins; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018514:Plant Structures / Q000187:drug effects / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D004791:Enzyme Inhibitors; D005030:Ethylenes; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; C007517:diphenyleneiodonium; C011006:jasmonic acid; C072239:methyl jasmonate; C036216:ethylene; D003513:Cycloheximide; D010749:Phosphoprotein Phosphatases; D002688:Chitinases; C005073:ethephon