[Establishment of a culture system of chick embryo for mouse tooth germ development].
OBJECTIVE
To establish a new culture system for mouse tooth germs in chick embryo.


METHODS
The mandibular first molar germ fragments of 15 embryonic days' Kunming mouse embryo were implanted into the lateral mesenchyme of 4-5 days' chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.


RESULTS
The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differentiated maturely and secreted matrix.


CONCLUSIONS
4-5 days' wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.
Yan
Zheng-bin
ZB
Key. Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Tian
Wei-dong
WD
Liu
Lei
L
Hou
Jin-qiu
JQ
Chen
Xi-zhe
XZ
Li
Zhi-yong
ZY
D000565:Ameloblasts; D000818:Animals; D002642:Chick Embryo; D051379:Mice; D008963:Molar / Q000196:embryology; D009804:Odontoblasts; D014083:Tooth Germ / Q000196:embryology*