Fasciola gigantica: immunization of rabbits with proteins isolated from coproantigen.
Two fractions were isolated from coproantigen by ion-exchange chromatography in which DEAE cellulose was utilized. Both fractions and crude antigen were characterized by SDS-polyacrylamide gel electrophoresis which revealed 13 bands of molecular weight ranged from 205-31 in crude coproantigen. While fraction I resolved into six bands of molecular weight 198, 178, 148, 111, 101 & 45. Fraction II showed seven bands of 191 KDa, 178KDa, 166KDa, 118KDa, 98.5KDa, 72KDa & 32KDa. Fraction 1I was higher immunoreactivity than fraction by ELISA. Three immunoreactive bands of 191KDa, 118KDa & 98.5KDa were identified in fraction II using immunoblot assay. Five bands of 178KDa, 148KDa, 111KDa, 101KDa & 45KDa were detected in fraction I. Immunization of rabbits twice with fraction II in Freund's adjuvant with two weeks interval followed by challenge with F. gigantica metacercariae resulted in 66.6% protection from infection. The protection was assessed by detect-ion of hepatic damage, worm recoveries and antibody response. High level of IgG response in vaccinated rabbits than control infected ones occurred and being responsible for the recorded protection.
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Eman H
EH
Abdel-Rahman
Kadria N
KN
Abdel-Megeed
D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D011817:Rabbits / Q000276:immunology / Q000469:parasitology*; D014611:Vaccination / Q000662:veterinary*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth