Mass spectrometric imaging of lipids in brain tissue.
The spatial distributions of various specific lipids in freeze-dried mouse brain sections were monitored using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Mouse brain sections were prepared by cryosectioning, rinsing in 0.15 M NH3HCOO, and freeze-drying, after which the samples were analyzed directly by TOF-SIMS, using Au3+ ions as primary ions. Positive and negative TOF-SIMS spectra of the tissue surface contained peaks from quasimolecular ions of a variety of specific lipids, including cholesterol, sulfatides, phosphatidylinositols, and phosphatidylcholines. Images showing the spatial signal intensity distributions of specific ions were recorded across analysis areas ranging from 100 x 100 microm(2) to 9 x 9 mm(2). The results demonstrate a highly complementary localization of cholesterol and phosphatidylcholine over dimensions from millimeter to micrometer range. Characteristic spatial distributions of several other lipids, including sulfatides and phosphatidylinositols, were observed. Principal component analysis was used to localize regions of the sample surface that show common spectral features. Spectra from different such regions showed large variations in lipid ion signals, indicating large variations in the lipid composition in different regions.
Department of Chemistry and Materials Technology, SP Swedish National Testing and Research Institute, P.O. Box 857, SE-50115 Borås, Sweden. peter.sjovall@sp.se
Peter
P
Sjövall
Jukka
J
Lausmaa
Björn
B
Johansson
D000818:Animals; D001923:Brain Chemistry; D002784:Cholesterol / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D051379:Mice; D010713:Phosphatidylcholines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D013433:Sulfoglycosphingolipids / Q000032:analysis
D008055:Lipids; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D013433:Sulfoglycosphingolipids; D002784:Cholesterol