In vitro microleakage of four tracers with multiple applications to the same tooth.
Microleakage testing continues to be undertaken using a variety of techniques and methodologies. This study compared four microleakage tracers to determine if a difference exists in their ability to demonstrate microleakage on a single dental amalgam restorative material by testing in two phases. Class V amalgam restorations were placed on the facial surfaces of 105 extracted human premolars with all margins in enamel. The teeth were stored at 37 degrees C in water for two weeks except during thermocycling for 2500 cycles between 8 degrees C and 48 degrees C. The teeth were prepared for microleakage testing by sealing the external surfaces with nail polish and tinfoil, leaving the restoration and surrounding 1 mm exposed. In the first phase, four groups of 15 teeth were randomly assigned to 0.5% basic fuchsin dye, 2.0% fluorescent dye, 1.5% reactive orange 14 and 45Ca. In the second phase, another three groups of 15 teeth were immersed in 45Ca, then immersed in one of the remaining three tracers. Ridit analysis and Newman-Keuls multiple comparisons were used to compare the groups at a significance level of 0.05. The results indicate that there are differences in observed microleakage between tracers and there is no statistical influence on dye tracers by initial immersion in 45Ca.
Cochran
Michael A
MA
Graduate Operative Dentistry, Indiana University School of Dentistry, Indianapolis, IN, USA.
Gonzales
Ma Angela G
MA
Platt
Jeffrey A
JA
Moore
B Keith
BK
D001641:Bicuspid; D002132:Calcium Radioisotopes; D004396:Coloring Agents; D003723:Dental Amalgam; D003763:Dental Leakage / Q000175:diagnosis*; D003793:Dental Restoration, Permanent; D005456:Fluorescent Dyes; D006801:Humans; D012394:Rosaniline Dyes; D018709:Statistics, Nonparametric; D014227:Triazines
D002132:Calcium Radioisotopes; D004396:Coloring Agents; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; D014227:Triazines; C053575:reactive orange 14; D003723:Dental Amalgam