A two-dimensional proteome map of maize endosperm.
We have established a proteome reference map for maize (Zea mays L.) endosperm by means of two-dimensional gel electrophoresis and protein identification with LC-MS/MS analysis. This investigation focussed on proteins in major spots in a 4-7 pI range and 10-100 kDa M(r) range. Among the 632 protein spots processed, 496 were identified by matching against the NCBInr and ZMtuc-tus databases (using the SEQUEST software). Forty-two per cent of the proteins were identified against maize sequences, 23% against rice sequences and 21% against Arabidopsis sequences. Identified proteins were not only cytoplasmic but also nuclear, mitochondrial or amyloplastic. Metabolic processes, protein destination, protein synthesis, cell rescue, defense, cell death and ageing are the most abundant functional categories, comprising almost half of the 632 proteins analyzed in our study. This proteome map constitutes a powerful tool for physiological studies and is the first step for investigating the maize endosperm development.
INRA/INA-PG/UPS/CNRS UMR8120, Ferme du Moulon, Gif-sur-Yvette, France.
Valérie
V
Méchin
Thierry
T
Balliau
Sophie
S
Château-Joubert
Marlène
M
Davanture
Olivier
O
Langella
Luc
L
Négroni
Jean-Louis
JL
Prioul
Claudine
C
Thévenot
Michel
M
Zivy
Catherine
C
Damerval
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D013058:Mass Spectrometry; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome