Monitoring of dnaK gene expression in Porphyromonas gingivalis by oxygen stress using DNA microarray.
Porphyromonas gingivalis, a Gram-negative anaerobe associated with adult periodontitis, expresses numerous potential virulence factors. dnaK, a member of the heat shock protein family, functions as a molecular chaperone and plays a role in microbial pathogenicity. However, little is known regarding its gene expression caused by oxygen stress in P. gingivalis. In the present study, a custom-made DNA microarray was designed and used to monitor dnaK gene expression in P. gingivalis caused by oxygen stress. The results demonstrated that dnaK mRNA was up-regulated in a short time, and the DNA microarray results were confirmed by real-time polymerase chain reaction analysis. These findings suggest that oxygen stress stimulates gene expression of dnaK and may have a relationship to the aerotolerance activity of this organism as well as its expression of pathogenesis.
Araki
Makoto
M
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Hiratsuka
Koichi
K
Kiyama-Kishikawa
Michiko
M
Abiko
Yoshimitsu
Y
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000235:genetics*; D010100:Oxygen / Q000494:pharmacology; D010518:Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000472:pathogenicity; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D018832:Molecular Chaperones; D037521:Virulence Factors; D000251:Adenosine Triphosphatases; C115251:DnaK protein, Brevibacillus choshinensis; D010100:Oxygen