Characterization of the mouse adeno-associated virus AAVS1 ortholog.
The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proximal to the recently identified Mbs85 gene. These sequence motifs can be specifically nicked by the viral Rep protein required for the initiation of site-specific AAV DNA integration. Furthermore, these signals can serve as a minimal origin for Rep-dependent DNA replication. In addition, we isolated the mouse Mbs85 proximal promoter and show transcriptional activity in three mouse cell lines.
Dutheil
Nathalie
N
Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, One Gustave L. Levy Pl., Box 1496, New York, NY 10029, USA.
Yoon-Robarts
Miran
M
Ward
Peter
P
Henckaerts
Els
E
Skrabanek
Lucy
L
Berns
Kenneth I
KI
Campagne
Fabien
F
Linden
R Michael
RM
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D016662:Virus Integration
D011506:Proteins