JNK MAP kinase is involved in the humoral immune response of the greater wax moth larvae Galleria mellonella.
We investigated the participation of MAP kinases in the response of Galleria mellonella larvae to immune challenge. JNK MAP kinase was activated in fat body 10-15 min after LPS injection in vivo. The level of JNK activation was time- and LPS dosage-dependent. JNK MAP kinase isolated from cell-free extract of fat bodies dissected from immune stimulated larvae phosphorylated c-Jun protein in vitro. The activity of Gm JNK kinase was abolished in the presence of the JNK specific inhibitor SP600125. Our data indicate a correlation between JNK phosphorylation and induction of antimicrobial activity in the insect hemolymph after immune stimulation. Hemolymph from larvae pre-treated with JNK specific inhibitor SP600125 showed a reduced level of antibacterial activity after LPS injection. JNK inhibition by SP600125 abolished antibacterial activity of the in vitro culture of G. mellonella fat body. Finally, we also show a correlation between JNK-dependent immune response of G. mellonella larvae and elevated temperature.
Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Sklodowska University, Lublin, Poland.
Iwona
I
Wojda
Patryk
P
Kowalski
Teresa
T
Jakubowicz
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D015153:Blotting, Western; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005216:Fat Body / Q000201:enzymology / Q000276:immunology; D006458:Hemolymph / Q000276:immunology; D048031:JNK Mitogen-Activated Protein Kinases; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System / Q000276:immunology*; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology* / Q000378:metabolism; D009036:Moths / Q000201:enzymology* / Q000276:immunology*; D010766:Phosphorylation
D000873:Anthracenes; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; C432165:pyrazolanthrone; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases