[Application of EITB in immunodiagnosis of cysticercosis].
OBJECTIVE
To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis.


METHODS
Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared.


RESULTS
Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14000 and 16600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB.


CONCLUSIONS
A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.
Department of Immunology, Weifang Medical College, Weifang 261042, China.
Li-na
LN
Wang
Ling-yun
LY
Ge
Feng
F
Miao
Zhen-hua
ZH
Yu
Yu-bing
YB
Liu
Tian-min
TM
Zhen
Gui-ping
GP
Li
Shu-fang
SF
Yang
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional; D007159:Immunologic Tests; D012680:Sensitivity and Specificity
D000947:Antigens, Helminth