Chromatin immunoprecipitation assays.
Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain. Here, we show the step-by-step methods currently used in our lab to immunoprecipitate the formaldehyde crosslinked chromatin and further analyze the immuprecipitated DNA by semiquantitative PCR.
Yan
Yan
Y
Department of Environmental Medical, New York University School of Medicine, Tuxedo, NY, USA.
Chen
Haobin
H
Costa
Max
M
D002843:Chromatin / Q000737:chemistry* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011233:Precipitin Tests / Q000379:methods*; D012995:Solubility; D013010:Sonication
D002843:Chromatin