Superoxide dismutase moderates basal and induced bacterial adherence and interleukin-8 expression in airway epithelial cells.
Bacterial infection of the tracheobronchial tree is a frequent, serious complication in patients receiving treatment with oxygen and mechanical ventilation, resulting in increased morbidity and mortality. Using human airway epithelial cell culture models, we examined the effect of hyperoxia on bacterial adherence and the expression of interleukin-8 (IL-8), an important mediator involved in the inflammatory process. A 24-h exposure to 95% O(2) increased Pseudomonas aeruginosa (PA) adherence 57% in A549 cells (P < 0.01) and 115% in 16HBE cells (P < 0.01) but had little effect on Staphylococcus aureus (SA) adherence. Exposure to hyperoxia, followed by a 1-h incubation with SA, further enhanced PA adherence (P < 0.01), suggesting that hyperoxia and SA colonization may enhance the susceptibility of lung epithelial cells to gram-negative infections. IL-8 expression was also increased in cells exposed to both hyperoxia and PA. Stable or transient overexpression of manganese superoxide dismutase reduced both basal and stimulated levels of PA adherence and IL-8 levels in response to exposure to either hyperoxia or PA. These data indicate that hyperoxia increases susceptibility to infection and that the pathways are mediated by reactive oxygen species. Therapeutic intervention strategies designed to prevent accumulation of intracellular reactive oxygen species may reduce opportunistic pulmonary infections.
Arita
Yuko
Y
CardioPulmonary Research Institute, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Winthrop-University Hospital, SUNY Stony Brook School of Medicine, Mineola, NY 11501, USA.
Joseph
Ansamma
A
Koo
Hshi-Chi
HC
Li
Yuchi
Y
Palaia
Thomas A
TA
Davis
Jonathan M
JM
Kazzaz
Jeffrey A
JA
D000230:Adenocarcinoma; D001422:Bacterial Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D018496:Hyperoxia; D016209:Interleukin-8 / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D020545:Respiratory Mucosa / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D016209:Interleukin-8; D011994:Recombinant Proteins; D010088:Oxidoreductases; D013482:Superoxide Dismutase