Neuronal nicotinic synapse assembly requires the adenomatous polyposis coli tumor suppressor protein.
Normal cognitive and autonomic functions require nicotinic synaptic signaling. Despite the physiological importance of these synapses, little is known about molecular mechanisms that direct their assembly during development. We show here that the tumor-suppressor protein adenomatous polyposis coli (APC) functions in localizing alpha3-nicotinic acetylcholine receptors (nAChRs) to neuronal postsynaptic sites. Our quantitative confocal microscopy studies indicate that APC is selectively enriched at cholinergic synapses; APC surface clusters are juxtaposed to synaptic vesicle clusters and colocalize with alpha3-nAChRs but not with the neighboring synaptic glycine receptors or perisynaptic alpha7-nAChRs on chick ciliary ganglion (CG) neurons. We identify PSD (postsynaptic density)-93, beta-catenin, and microtubule end binding protein EB1 as APC binding partners. PSD-93 and beta-catenin are also enriched at alpha3-nAChR postsynaptic sites. EB1 shows close proximity to and partial overlap with alpha3-nAChR and APC surface clusters. We tested the role of APC in neuronal nicotinic synapse assembly by using retroviral-mediated in vivo overexpression of an APC dominant-negative (APC-dn) peptide to block the interaction of endogenous APC with both EB1 and PSD-93 during synapse formation in CG neurons. The overexpressed APC-dn led to dramatic decreases in alpha3-nAChR surface levels and clusters. Effects were specific to alpha3-nAChR postsynaptic sites; synaptic glycine receptor and perisynaptic alpha7-nAChR clusters were not altered. In addition, APC-dn also reduced surface membrane-associated clusters of PSD-93 and EB1. The results show that APC plays a key role in organizing excitatory cholinergic postsynaptic specializations in CG neurons. We identify APC as the first nonreceptor protein to function in localizing nAChRs to neuronal synapses in vivo.
Temburni
Murali Krishna
MK
Department of Neuroscience, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Rosenberg
Madelaine M
MM
Pathak
Narendra
N
McConnell
Russell
R
Jacob
Michele H
MH
D025601:Adenomatous Polyposis Coli Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002642:Chick Embryo; D002799:Cholinergic Fibers / Q000737:chemistry / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000196:embryology; D017491:Genes, APC; D007395:Interneurons / Q000737:chemistry / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D018009:Receptors, Glycine / Q000032:analysis; D011978:Receptors, Nicotinic / Q000032:analysis / Q000737:chemistry* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013569:Synapses / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D015534:Trans-Activators / Q000032:analysis; D020798:Two-Hybrid System Techniques; D051176:beta Catenin
D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D018009:Receptors, Glycine; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D051176:beta Catenin; C033652:postsynaptic density proteins