Preparation of dodecanol-tolerant strains of Yarrowia lipolytica.
Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strain MTLY35. These strains grew in rich media containing up to 10% (v/v) dodecanol. Dodecanol-tolerant strains remained dodecanol tolerant after they had been cultured in rich media without dodecanol. No significant amount of dodecanedioic acid was accumulated by the dodecanol-tolerant strains when grown on glucose in the presence of dodecanol.
Smit
Martha S
MS
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O Box 339, Bloemfontein 9300, South Africa. smitms.sci@mail.uovs.ac.za
Mokgoro
Masego M
MM
Setati
Evodia
E
Nicaud
Jean-Marc
JM
D042962:Acyl-CoA Oxidase / Q000172:deficiency* / Q000235:genetics; D000222:Adaptation, Physiological / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D007851:Dodecanol / Q000494:pharmacology*; D004361:Drug Tolerance / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D013045:Species Specificity; D025062:Yarrowia / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*
D007851:Dodecanol; D042962:Acyl-CoA Oxidase