[Antioxidative activities of two metabolites of cultured marine fungus, Halorosellinia oceanicum 323 in vitro].
OBJECTIVE
To investigate the antioxidative effects of 323-A and 323-B, two isomers extracted from the metabolites of cultured marine fungus, Halorosellinia oceanicum 323 in vitro.


METHODS
NADH-PMS-NBT system was used to produce superoxide free radical (O2*-), EDTANa2-Fe(II)-H2O2 system to generate hydroxyl free radical (*OH), H2O2 to stimulate oxidative hemolysis of erythrocytes of rats, Cys-Fe2+ to induce malondialdehyde (MDA) production in homogenates, and ferrous-ascorbic acid system to increase the turbidity of mitochondria suspension in the liver of rats. And the antioxidative activities of 323-A and 323-B were studied.


RESULTS
323-A and 323-B not only scavenge O2*- and *OH produced by the experimental systems directly, but also inhibit H2O2 stimulated oxidative hemolysis of erythrocytes of rats, depress MDA production in homogenates induced by Cys-Fe2+ system, and reduce the turbidity of mitochondria suspension in the liver of rats increased by ferrous-ascorbic acid system in vitro.


CONCLUSIONS
323-A and 323-B showed comprehensive cleaning actions on free radicals and protective effects on the functions of tissues and cells against oxidative lesion. The results suggested that the marine microorganic metabolites might be a novel and profound source of antioxidative reagents.
School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275.
Jinghui
J
Luo
Yingbao
Y
Yang
Yongcheng
Y
Lin
Zhiliang
Z
Chen
Guangce
G
Jiang
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D017665:Hydroxyl Radical / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D013481:Superoxides; D017665:Hydroxyl Radical; D008315:Malondialdehyde