Interaction between a negative regulator (Msa2/Nrd1) and a positive regulator (Cpc2) of sexual differentiation in Schizosaccharomyces pombe.
The sexual differentiation of Schizosaccharomyces pombe is controlled by many cellular components which have not been fully characterized. We isolated a gene called msa2 as a multi-copy suppressor of a sporulation abnormal mutant (sam1). Msa2p is identical with Nrd1p which has been characterized as a factor that blocks the onset of sexual differentiation. The yeast two-hybrid system was used to identify Cpc2p, a fission yeast homolog of the RACK1 protein, that interacted with Msa2p/Nrd1p. We confirmed that Msa2p/Nrd1p interacted with Cpc2p in S. pombe cells. An epistatic analysis of msa2/nrd1 and cpc2 suggests that Msa2p/Nrd1p was an upstream regulator for Cpc2p. A localization analysis of Cpc2p and Msa2p/Nrd1p indicates that both proteins were predominantly localized in the cytoplasm. The interaction of negative regulator Msa2p/Nrd1p with positive regulator Cpc2p suggests a new regulatory circuit in the sexual differentiation of S. pombe.
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue 690-8504, Japan.
Hee Tae
HT
Jeong
Yasuo
Y
Oowatari
Michiyo
M
Abe
Katsunori
K
Tanaka
Hideyuki
H
Matsuda
Makoto
M
Kawamukai
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D049452:Green Fluorescent Proteins; D008856:Microscopy, Fluorescence; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins; D012261:Ribonucleoproteins / Q000235:genetics / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic / Q000502:physiology; D020798:Two-Hybrid System Techniques
D004271:DNA, Fungal; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D029702:Schizosaccharomyces pombe Proteins; C000617313:cpc2 protein, S pombe; C491210:nrd1 protein, S pombe; D049452:Green Fluorescent Proteins