Determination of alkanolamines in cattails (Typha latifolia) utilizing electrospray ionization with selected reaction monitoring and ion-exchange chromatography.
Selected reaction monitoring (SRM) with electrospray ionization was used as a specific detection technique for the analysis of alkanolamines in plant tissue extracts. Ion-exchange chromatography was used as the method of separation. Quantification was based on monitoring the loss of either H2O or 2(H2O) from the protonated molecule [M+H]+. The method provided increased selectivity for all analytes and better detection limits for three of the six analytes investigated compared with an earlier method using selected ion monitoring with liquid chromatography. Instrumental detection limits ranged from 6-300 pg injected for monoethanolamine (MEA), monoisopropanolamine (MIPA), diethanolamine (DEA), methyldiethanolamine (MDEA), diisopropanolamine (DIPA), and triethanolamine (TEA). Method robustness and selectivity were demonstrated by the determination of DIPA and a known transformation product MIPA in over 35 plant extract samples derived from a laboratory study of plant uptake mechanisms.
Environment Canada, National Water Research Institute, Saskatoon, SK, Canada.
Kerry M
KM
Peru
John V
JV
Headley
William J
WJ
Doucette
D000605:Amino Alcohols / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014020:Tissue Extracts / Q000737:chemistry; D032401:Typhaceae / Q000737:chemistry*
D000605:Amino Alcohols; D014020:Tissue Extracts