Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation.
A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.
Suzuki
Nao
N
Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama, Japan.
Nakano
Yoshio
Y
Yoshida
Akihiro
A
Yamashita
Yoshihisa
Y
Kiyoura
Yusuke
Y
D000190:Actinomyces / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D018441:Biofilms; D003199:Computer Systems; D017931:DNA Primers; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D017931:DNA Primers; D015345:Oligonucleotide Probes