Crystallization and preliminary X-ray analysis of D-monellin.
D-Monellin is a chemically synthesized protein composed of all D-amino acids. It has an amino-acid sequence identical to L-monellin, a natural protein with potent sweetness. Two crystal forms of D-monellin were obtained. Both crystals were grown under conditions similiar to those used to crystallize natural L-monellin. Crystal form I has similar, but not identical, cell parameters to natural L-monellin and diffracts to 2.7 A resolution. Crystal form II is very different and diffracts to 1.7 A resolution using synchrotron radiation.
Graduate Group in Biophysics, Department of Chemistry and Lawrence Berkeley National Laboratory, University of California, Berkeley 94720, USA.
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Hung
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Kohmura
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Ariyoshi
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Kim