Effect of defibrillation pulses of different shapes on biomembranes: experimental study.
We studied the effects of high-voltage single, double unipolar, and double bipolar electric pulses of exponential or sine shape on erythrocyte membranes. Either single or double (mono- or bipolar) pulses were used. All pulses electroporated the membranes, and the electroporation threshold did not depend on the pulse shape. Two successive pulses decreased erythrocyte number in a nonadditive way. Similar to defibrillation of the whole heart, the effect of two bipolar pulses on erythrocytes was more pronounced than the effect of two unipolar pulses.
Moroz
V V
VV
Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia.
Bogushevich
M S
MS
Chernysh
A M
AM
Kozlova
E K
EK
Sharakshane
A S
AS
D004554:Electric Countershock / Q000009:adverse effects* / Q000379:methods; D004594:Electrophysiology; D018274:Electroporation; D004910:Erythrocyte Membrane / Q000502:physiology*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics