Lack of influence of the anaerobic [NiFe] hydrogenase and L-1,2 propanediol oxidoreductase on the outcome of Actinobacillus pleuropneumoniae serotype 7 infection.
The genes for the large subunit of [NiFe] hydrogenase 2, (hybB) and for L-1,2 propanediol oxidoreductase (fucO), were identified in an Actinobacillus (A.) pleuropneumoniae serotype 7 strain. Based on the hypothesis that adaptation to anaerobic conditions in damaged lung tissue may play a role in A. pleuropneumoniae persistence in host tissues, deletion mutants with a deletion in the hybB or the fucO gene were constructed and examined in an aerosol infection model. Deletion of the hybB or fucO genes appeared to have no significant effect on A. pleuropneumoniae virulence.
Baltes
Nina
N
Department of Infectious Diseases, Institute of Microbiology, School of Veterinary Medicine, Infectious Diseases of Animals, Bischofsholer Damm 15, Hannover D-30173, Germany. nbaltes@gmx.de
Kyaw
Sunn
S
Hennig-Pauka
Isabel
I
Gerlach
Gerald-F
GF
D000189:Actinobacillus Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D000693:Anaerobiosis / Q000276:immunology; D000818:Animals; D001831:Body Temperature / Q000276:immunology; D006864:Hydrogenase / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008221:Lymphoid Tissue / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016254:Mutagenesis, Insertional; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology*
D000429:Alcohol Oxidoreductases; C021494:lactaldehyde reductase; C043296:nickel-iron hydrogenase; D006864:Hydrogenase