Effects of glucose on fsr-mediated biofilm formation in Enterococcus faecalis.
Biofilm production is frequently dependent on such environmental factors as cell density and glucose concentration. The Enterococcus faecalis quorum-sensing locus (fsr) increases enterococcal virulence in multiple animal models. To date, fsr has been shown to regulate the transcription of 2 downstream protease genes. We demonstrate that the effect of fsr mutations on biofilm formation, as well as the fsr-mediated catabolite control of biofilm, is mediated via these proteases. The present study provides additional insight into the mechanisms used by E. faecalis to establish nosocomial infection.
Pillai
Satish K
SK
Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA. spillai@bidmc.harvard.edu
Sakoulas
George
G
Eliopoulos
George M
GM
Moellering
Robert C
RC
Murray
Barbara E
BE
Inouye
Roger T
RT
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D009154:Mutation
D001426:Bacterial Proteins; C409031:FsrA protein, Enterococcus faecalis; D010450:Endopeptidases; D005947:Glucose