A reversible adsorption-desorption interface of DNA based on nano-sized zirconia and its application.
It is essential for the information storage in DNA-based bio-chips to construct a reversible exchange interface of DNA. Here, a highly reproducible and reversible adsorption-desorption interface of DNA based on the nano-sized zirconia in different pH solution was successfully fabricated. The results showed that DNA can be adsorbed onto the nano-sized zirconia from its solution, and can desorb from the nanoparticles in 0.10 M KOH solution. When the matrix with nanoparticles returns to the DNA solution again, DNA can be re-adsorbed onto them as initial state. Moreover, the interaction of DNA with non-electroactive molecules, 2,2'-bipyridine, has been studied by electrochemistry method in the aid of probe Co(phen)(3)(3+). The experiments showed that when 2,2'-bipyridine was added into the test solution, the voltammetric peak currents of Co(phen)(3)(3+) decreased; and the decrease value of peak current against the concentration of 2,2'-bipyridine has a good Langmuir relationship, by which the equilibrium constant of interaction between 2,2'-bipyridine and DNA was estimated to be 1.57 x 10(4)M(-1).
The Laboratory of Life Analytical Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093, China.
Shou-Qing
SQ
Liu
Jing-Juan
JJ
Xu
Hong-Yuan
HY
Chen
D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D036103:Nanotechnology; D015040:Zirconium / Q000737:chemistry*
D004247:DNA; D015040:Zirconium; C028541:zirconium oxide