An integrated method for mutation detection using on-chip sample preparation, single-stranded conformation polymorphism, and heteroduplex analysis.
This work integrates rapid techniques for mutation detection by producing single-stranded DNA and (renatured) double-stranded DNA on-chip, labeling these with fluorescent DNA stains and then performing two complementary methods of mutation detection-single stranded conformation polymorphism (SSCP) analysis and heteroduplex analysis (HA). This involves the denaturation of double-stranded polymerase chain reaction (PCR) product into single-stranded DNA, the mutation analysis of the single-stranded DNA by SSCP and the rehybridized double-stranded DNA by HA. These steps were performed entirely on-chip within several minutes of operation. The combination of these two mutation detection methods on-chip provides a highly sensitive method of mutation detection for either genotyping or screening. Many mutation analysis methods rely upon fluorescently labeled samples from a PCR with fluorescently labeled primers. By labeling on-chip we not only attain improved signal strength, but the method is considerably more versatile. Although we used PCR products in this work, this method could be used to analyze DNA from any source. We believe that this combination of several procedures on a single chip represents a significant step in the development of higher levels of integration upon microfluidic devices.
Vahedi
Golnaz
G
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada.
Kaler
Karan
K
Backhouse
Christopher J
CJ
D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D046208:Microchip Analytical Procedures / Q000379:methods*; D009154:Mutation / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes