Application of ion-trap LC/MS/MS for determination of acrylamide in processed foods.
Ion-trap LC/MS/MS was evaluated for use in the determination of acrylamide (AA) in processed foods. Multiple reaction monitoring (MRM) analysis of a series of AA standard solutions containing deuterium-labeled acrylamide (AA-d3) as an internal standard was performed. A linear relationship between the concentration of AA and the ratio of peak area (AA/AA-d3) in the extracted ion chromatogram (m/z 55, 58 derived from m/z 72, 75, respectively) was obtained over a wide range of 2-20,000 ng/mL. The quantification limit of AA was 2 ng/mL. In analyses of 37 commercial foods, AA was detected in a potato snack at the maximum value of 3,570 ng/g and found in 23 foods prepared or cooked at high temperature. The samples were analyzed in triplicate and the relative standard deviations (RSD) were less than 15% in many processed foods.
Tsutsumiuchi
Kaname
K
College of Bioscience & Biotechnology, Chubu University, 1200, Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Hibino
Mariko
M
Kambe
Mariko
M
Oishi
Kaori
K
Okada
Masahiko
M
Miwa
Johji
J
Taniguchi
Hajime
H
D020106:Acrylamide / Q000032:analysis*; D002853:Chromatography, Liquid; D005504:Food Analysis / Q000379:methods*; D013058:Mass Spectrometry
D020106:Acrylamide