Scavenger effect of flavonols on HOCl-induced luminol chemiluminescence.
Hypochlorous acid (HOCl), the main product of the myeloperoxidase system, is a strong oxidant and a potent chlorinating agent, which can damage host tissues. In the present work, the scavenger effect of three aglycone flavonols (myricetin, quercetin and kaempferol) and of the natural glycoside flavonol, rutin, was studied towards HOCl using luminol-dependent chemiluminescence (CL). At 1 micro mol/L fi nal concentration, rutin was the most powerful scavenger of HOCl with an inhibitory luminol oxidation of 91.4% +/- 3.2%. Quercetin, kaempferol and myricetin inhibited the luminol-dependent CL at the same concentration only by 75.9% +/- 3.4%, 57.7% +/- 5.3% and 43.3% +/- 3.5%, respectively. With increasing concentration of these flavonols, a dose-dependent inhibition of luminol CL was observed. In order to prove to what extent flavonols scavenge HOCl, their concentrations that gave 50% inhibition of luminescence (IC50) were compared to IC50 values of the sulphur-containing compounds N-acetyl cysteine (NAC) and taurine. The scavenging activities of compounds tested decrease in the order: rutin > NAC > quercetin > kaempferol > taurine. The present study revealed that rutin was the most effective scavenger agent.
Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat ABBAS, 19000 Setif, Algeria.
Laïd
L
Selloum
Hanifa
H
Djelili
Laïla
L
Sebihi
Juergen
J
Arnhold
D000111:Acetylcysteine / Q000737:chemistry; D044948:Flavonols / Q000737:chemistry*; D006997:Hypochlorous Acid / Q000737:chemistry*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry
D044948:Flavonols; D008165:Luminol; D012431:Rutin; D006997:Hypochlorous Acid; D000111:Acetylcysteine