[Disinfectant tests at 20 and 10 degrees C to determine the virucidal activity against circoviruses].
To evaluate virucidal activity against porcine circovirus type 2 (PCV2), four disinfectants were tested under laboratory conditions. As basis to perform the testing the "Guidelines for testing chemical disinfectants" of the German Veterinary Association (DVG-guidelines) were applied. For simulation of field conditions, the tests were carried out in virus carrier tests, at 20 and 10 degrees C, and under protein load (40% foetal calf serum (FCS) in virus suspension). For disinfection of PCV2 at 20 degrees C an exposure time of 120 min in 2% Disinfectant 1 (20% glutaraldehyde, 12% 2-propenal, polymer with formaldehyde) or Disinfectant 2 (55% formic acid, 7% glyoxylic acid) was necessary. 1% of Disinfectant 3 (Component 1: Potassium peroxomonosulphate. Component 2: Active detergents) disinfected PCV2 on carriers within 180 min. After a reaction time of 120 min with 1% and 60 min with 2% Disinfectant 4 (21% glutaraldehyde, 17% formaldehyde) there could not be detected any virus. Reduction on effectivity through temperature reduction to 10 degrees C were more significant for aldehyde containing preparations Disinfectant 1 and Disinfectant 4 than for Disinfectant 2 and Disinfectant 3. These losses on effectivity could be corrected through extension of exposure time or increase of concentration.
Klinik für Vögel, Reptilien, Amphibien und Fische, Justus-Liebig-Universität Giessen. ayhan.yilmaz@vetmed.uni-giessen.de
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D000818:Animals; D018173:Circoviridae Infections / Q000188:drug therapy / Q000517:prevention & control / Q000662:veterinary; D017925:Circovirus / Q000187:drug effects* / Q000254:growth & development; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006920:Hygiene; D007700:Kinetics; D013553:Swine Diseases / Q000188:drug therapy / Q000517:prevention & control; D013696:Temperature; D016896:Treatment Outcome
D004202:Disinfectants