In vivo flow cytometry: a new method for enumerating circulating cancer cells.
The fate of circulating tumor cells is an important determinant of their ability to form distant metastasis. Here, we demonstrate the use of in vivo flow cytometry as a powerful new method for detecting quantitatively circulating cancer cells. We specifically examine the circulation kinetics of two prostate cancer cell lines with different metastatic potential in mice and rats. We find that the cell line and the host environment affect the circulation kinetics of prostate cancer cells, with the intrinsic cell line properties determining the initial rate of cell depletion from the circulation and the host affecting cell circulation at later time points.
Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, 40 Blossom Street, BHX630, Boston, MA 02114, USA.
Irene
I
Georgakoudi
Nicolas
N
Solban
John
J
Novak
William L
WL
Rice
Xunbin
X
Wei
Tayyaba
T
Hasan
Charles P
CP
Lin
D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000097:blood / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured