Jamip1 (marlin-1) defines a family of proteins interacting with janus kinases and microtubules.
Jamip1 (Jak and microtubule interacting protein), an alias of Marlin-1, was identified for its ability to bind to the FERM (band 4.1 ezrin/radixin/moesin) homology domain of Tyk2, a member of the Janus kinase (Jak) family of non-receptor tyrosine kinases that are central elements of cytokine signaling cascades. Jamip1 belongs to a family of three genes conserved in vertebrates and is predominantly expressed in neural tissues and lymphoid organs. Jamip proteins lack known domains and are extremely rich in predicted coiled coils that mediate dimerization. In our initial characterization of Jamip1 (73 kDa), we found that it comprises an N-terminal region that targets the protein to microtubule polymers and, when overexpressed in fibroblasts, profoundly perturbs the microtubule network, inducing the formation of tight and stable bundles. Jamip1 was shown to associate with two Jak family members, Tyk2 and Jak1, in Jurkat T cells via its C-terminal region. The restricted expression of Jamip1 and its ability to associate to and modify microtubule polymers suggest a specialized function of these proteins in dynamic processes, e.g. cell polarization, segregation of signaling complexes, and vesicle traffic, some of which may involve Jak tyrosine kinases.
Unité de Signalisation des Cytokines, CNRS URA 1961, Cedex 15, France.
Corinna
C
Steindler
Zhi
Z
Li
Michèle
M
Algarté
Andrès
A
Alcover
Valentina
V
Libri
Josiane
J
Ragimbeau
Sandra
S
Pellegrini
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D019992:Databases as Topic; D003902:Detergents / Q000494:pharmacology; D019281:Dimerization; D005347:Fibroblasts / Q000378:metabolism; D017930:Genes, Reporter; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D053613:Janus Kinase 1; D019169:Jurkat Cells; D008156:Luciferases / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011061:Poly A / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D053634:TYK2 Kinase; D013997:Time Factors; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D003902:Detergents; C484851:JAKMIP1 protein, human; D011108:Polymers; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011061:Poly A; D012313:RNA; D008156:Luciferases; D005982:Glutathione Transferase; D011505:Protein-Tyrosine Kinases; C507904:JAK1 protein, human; D053613:Janus Kinase 1; D053634:TYK2 Kinase; C070139:TYK2 protein, human