In vitro conidial germination in Arthrinium aureum and Arthrinium phaeospermum.
This paper describes the microscopic details of conidial germination and the influence of pH, sodium chloride concentration, 3% glucose, 3% saccharose and ultraviolet irradiation on the conidial germination in Arthrinium species. Under laboratory conditions, germination started after an incubation period of 90 minutes in 2% malt extract broth at 25 degrees C. In vitro, the conidia of Arthrinium species have a very low percentage of germination (A. phaeospermum: 7.9%; A. aureum: 15.8%). Conidia of this genus have a characteristic equatorial slit. Conidia may break spontaneously at this slit, releasing their cytoplasmic contents. Arthrinium phaeospermum attains its optimum germination percentage when its conidia are suspended in a sterile saline solution (pH 3.5) in a water bath at 20 degrees C for 15 minutes before being inoculated on 2% malt extract agar. Conidial suspensions of A. aureum may be held in the same conditions, but for 30 minutes.
Agut
Montserrat
M
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. magut@iqs.edu
Calvo
María Angeles
MA
D001203:Ascomycota / Q000254:growth & development* / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012965:Sodium Chloride / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014466:Ultraviolet Rays
D012965:Sodium Chloride; D013395:Sucrose; D005947:Glucose