Evaluation of a new automated, standardized generic nucleic acid extraction method (total nucleic acid isolation kit) used in combination with cytomegalovirus DNA quantification by COBAS AMPLICOR CMV MONITOR.
The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized, and time-saving tool for DNA extraction.
Gärtner
Barbara Christine
BC
Institute of Virology, University of the Saarland, Homburg/Saar, Haus 47, D-66421 Homburg/Saar, Germany. vibgae@uniklinik-saarland.de
Fischinger
Johannes Michael
JM
Litwicki
Anke
A
Roemer
Klaus
K
Mueller-Lantzsch
Nikolaus
N
D001331:Automation / Q000379:methods; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011183:Postoperative Complications / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014180:Transplantation / Q000009:adverse effects
D004279:DNA, Viral; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic