Allosteric regulation and temperature dependence of oxygen binding in human neuroglobin and cytoglobin. Molecular mechanisms and physiological significance.
Two new globin proteins have recently been discovered in vertebrates, neuroglobin in neurons and cytoglobin in all tissues, both showing heme hexacoordination by the distal His(E7) in the absence of gaseous ligands. In analogy to hemoglobin and myoglobin, neuroglobin and cytoglobin are supposedly involved in O2 storage and delivery, although their physiological role remains to be solved. Here we report O2 equilibria of recombinant human neuroglobin (NGB) and cytoglobin (CYGB) measured under close to physiological conditions and at varying temperature and pH ranges. NGB shows both alkaline and acid Bohr effects (pH-dependent O2 affinity) and temperature-dependent enthalpy of oxygenation. O2 and CO binding equilibrium studies on neuroglobin mutants strongly suggest that the bound O2 is stabilized by interactions with His(E7) and that this residue functions as a major Bohr group in the presence of Lys(E10). As shown by the titration of free thiols with 4,4'-dithiodipyridine and by mass spectrometry, this mechanism of modulating O2 affinity is independent of formation of an internal disulfide bond under the experimental conditions used, which stabilize thiols in the reduced form. In CYGB, O2 binding is cooperative, consistent with its proposed dimeric structure. Similar to myoglobin but in contrast to NGB, O2 binding to CYGB is pH-independent and exothermic throughout the temperature range investigated. Our data support the hypothesis that CYGB may be involved in O2-requiring metabolic processes. In contrast, the lower O2 affinity in NGB does not appear compatible with a physiological role involving mitochondrial O2 supply at the low O2 tensions found within neurons.
Department of Zoophysiology, Institute of Biological Sciences, C. F. Møllers Alle 131, Aarhus University, DK-8000 Aarhus C, Denmark. angela.fago@biology.au.dk
Angela
A
Fago
Christian
C
Hundahl
Sylvia
S
Dewilde
Kambiz
K
Gilany
Luc
L
Moens
Roy E
RE
Weber
D000495:Allosteric Site; D000818:Animals; D002248:Carbon Monoxide / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000078842:Cytoglobin; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D005914:Globins / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D013058:Mass Spectrometry; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D000078787:Neuroglobin; D009474:Neurons / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D013997:Time Factors
C000627265:CYGB protein, human; C000627267:Cygb protein, mouse; D000078842:Cytoglobin; D004220:Disulfides; D008024:Ligands; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D011725:Pyridines; D011994:Recombinant Proteins; C007542:4,4'-dipyridyl disulfide; D006639:Histidine; D002248:Carbon Monoxide; D005914:Globins; D008239:Lysine; D010100:Oxygen