Concentration estimation via curve fitting: quantification of negative inotropic agents by using a simple mathematical method in guinea pig atria.
We created a simple method based on curve fitting in order to assess the concentration of pharmacological agonists or antagonists in the microenvironment of the receptors. We tested our method in electrically driven guinea pig left atria by estimating the concentration of N6-cyclopentyladenosine (CPA; A1 adenosine receptor agonist), acetyl-beta-methylcholine (muscarinic receptor agonist) and verapamil (L-type Ca2+ channel inhibitor) added previously to the atria in known amounts. Our results validated the fitness of the model under specified conditions. In addition, our data suggest a relatively slow elimination of CPA in isolated, practically bloodless guinea pig atrial myocardium.
Department of Pharmacology and Pharmacotherapy, University of Debrecen, H-4012 Debrecen, PO Box 12, Hungary. gehlru@king.pharmacol.dote.hu
Rudolf
R
Gesztelyi
Judit
J
Zsuga
Béla
B
Juhász
Péter
P
Dér
Miklós
M
Vecsernyés
A József
AJ
Szentmiklósi
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003864:Depression, Chemical; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006325:Heart Atria / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology*; D018721:Muscarinic Agonists / Q000494:pharmacology; D009200:Myocardial Contraction / Q000187:drug effects*; D018047:Receptors, Purinergic P1 / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D018721:Muscarinic Agonists; D018047:Receptors, Purinergic P1; D016210:Methacholine Chloride; C048599:N(6)-cyclopentyladenosine; D014700:Verapamil; D000241:Adenosine