RScore: a peptide randomicity score for evaluating tandem mass spectra.
RScore, a new criterion of randomicity for evaluating tandem mass (MS/MS) spectra, is described. RScore is defined as the relative quality in cross-correlation and matched intensity percentage of a potentially positive peptide to those of other possible candidates for the same spectrum. By utilizing RScore combined with less stringent SEQUEST score filters, the number of true positive peptides can be increased and the number of false positives in datasets from a known protein mixture can be reduced compared with current SEQUEST parameters used alone. This algorithm is simple and adds little overheads to SEQUEST computation.
Li
Fuxin
F
Laboratory of Complex Systems and Intelligence Science, Institute of Automation, Chinese Academy of Sciences, Beijing 100080, China. lifuxin2001@yahoo.com.cn
Sun
Wei
W
Gao
Youhe
Y
Wang
Jue
J
D000465:Algorithms; D000818:Animals; D002417:Cattle; D013058:Mass Spectrometry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000145:classification; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D019151:Peptide Library; D010455:Peptides; D011506:Proteins