[Editing of the mitochondrial cox3 transcript may yield a new site of protein-protein interactions in wild cereal Elymus sibiricus L].
With PCR, RT-PCR, and direct sequencing, complete nucleotide sequences were established for the Elymus sibiricus mitochondrial cytochrome c oxidase subunit 3 gene (cox3) and its cDNA. The cox3 transcript was shown to have 12 editing sites with changes affecting the amino acid sequence of the protein product. The editing of the primary cox3 transcript was found to change the position of a site of protein-protein interactions. The results demonstrate again the important role of mRNA editing in posttranscriptional regulation of the expression of plant mitochondrial genes.
Tauson
E L
EL
Verbitskiĭ
D S
DS
Klimenko
E S
ES
Konstantinov
Iu M
IuM
Kulinchenko
M V
MV
Arziev
A Sh
ASh
Kobzev
V F
VF
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D018744:DNA, Plant; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D031749:Elymus / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018744:DNA, Plant; D008565:Membrane Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C093750:COX3 protein, S cerevisiae; D003576:Electron Transport Complex IV