[HIV-1 serotyping of V3-mimicking peptides circulating in the Republic of Tajikistan among intravenous drug users].
The paper summarizes the results of HIV-1 serotyping by using 56 positive sera collected in the territory of the Republic of Tajikistan (RT) from intravenous drug users (IVDU). It was made by solid-phase ELISA based on synthetic peptides, mimicking different variants of the apical epitope of the HIV-1 gp120 V3-loop. Two types of conjugates, those specific to human IgG and IgA, were used to detect the immune complexes. Serotypes, as determined according to IgG and IgA-based ELISA, coincided, however, the latter were proven to be more suitable for serotyping. There is a high level of HIV-1 serotype heterogeneity among IVDU in RT; altogether, 4 serotypes were identified, i.e. B (10%), B+A/C (18%), A/C (20%) and A/C+B (52%). The modern serotype HIV-1 diversity in RT resembles the epidemiologic situation in the territory of the former USSR as observed in the late 80-ies-early 90-ies of the last century.
Burunova
V V
VV
Shchelkanov
M Iu
MIu
Tishkova
F Kh
FKh
Starikov
N S
NS
Pazilin
A S
AS
Belobrova
E A
EA
Petrenko
M S
MS
Iudin
A N
AN
Samuilov
V D
VD
Garaev
M M
MM
L'vov
D K
DK
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D006679:HIV Seropositivity / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008433:Mathematics; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D012703:Serotyping; D013045:Species Specificity; D015819:Substance Abuse, Intravenous / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D013620:Tajikistan / Q000453:epidemiology
D000939:Epitopes; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010446:Peptide Fragments