[Function of actin in renal tubular epithelial cell of newborn swine during ATP deficiency].
OBJECTIVE
To investigate the variations of actin of newborn porcine Renal Tubalar Epithelial (RTE) cell during ATP deficiency and shed light on the possible mechanisms of renal deficiency during newborn asphyxia.


METHODS
After the establishment of ATP deficiency cell-model, the ATP content of the RTE cell of the control and hypoxic groups was determined. And the variations of actin in newborn porcine RTE cell during ATP deficiency were detected using flow-cytometry.


RESULTS
It was found that the ATP content of new-born porcine RTE cell decreased with the continuance of hypoxia, and the changes in G-actin and F-actin contents of RTE cell both appeared at the time of one-minute ATP deficiency. The G-actin decreased first and then increased, and the F-actin decreased step by step.


CONCLUSIONS
As the ATP deficiency time elongated, G-actin of the newborn porcine RTE cell decreased first and then increased, and the F-actin decreased step by step. This may destruct the cell bone-skeleton of the newborn RTE cell and maybe one of the important mechanisms of renal deficiency during newborn asphyxia.
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Da-peng
DP
Chen
Yu-jia
YJ
Yao
Juan
J
Chen
D000199:Actins / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000172:deficiency / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000378:metabolism; D015687:Cell Hypoxia; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology; D013552:Swine
D000199:Actins; D000255:Adenosine Triphosphate