A new approach for rapidly reshaping single-chain antibody in vitro by combining DNA shuffling with ribosome display.
Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti-4-1BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping.
Wang
Xiang-Bin
XB
Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China.
Zhou
Bing
B
Yin
Chang-Cheng
CC
Lin
Qing
Q
Huang
Hua-Liang
HL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D032621:DNA Shuffling; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D012270:Ribosomes / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment
D007135:Immunoglobulin Variable Region; D019151:Peptide Library