[Genetic polymorphisms of three STR loci on chromosome X and their forensic application in a Chinese Han population].
OBJECTIVE
To understand the allele structure and genetic polymorphism at three STR loci on chromosome X in Chinese Han population and make an evalution of their forensic application.


METHODS
EDTA-blood samples were collected from the unrelated individuals in Chengdu, China. After being extracted with Chelex method, the DNA samples were amplified by PCR technique. The PCR products were analyzed by PAG electrophoresis and the approach of automated fluorescence detection. Hardy-Weinberg equilibrium of females was tested and every forensic interested value was calculated.


RESULTS
The polymorphisms of all 3 STR loci were obtained from 100 unrelated females and 120 unrelated males from Chinese Han ethnic group. Chi-square tests on the genotype frequencies in females did not reveal deviations from Hardy-Weinberg equilibrium.


CONCLUSIONS
The obtained data are beneficial to understanding the population genetics of the three STR loci in Chinese Han population. For forensic genetics, the obtained data can be used to calculate the probabilities dealing with the paternity test and the individual identification.
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Mei-sen
MS
Shi
Bin-wu
BW
Ying
Jian-qiang
JQ
Deng
Ying-bi
YB
Li
Jin
J
Wu
Jing
J
Yan
Ji
J
Zhang
Yi-ping
YP
Hou
D002681:China / Q000208:ethnology; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences