[Construction of DNA vaccine of Trichinella spiralis muscle larvae and its expression in mice].
OBJECTIVE
To construct and express recombinant plasmid containing the structural gene encoding Mr 31 000 antigen of Trichinella spiralis muscle larvae.


METHODS
The target gene TspE1 was amplified by RT-PCR, cloned into pUC18 vector, and sub-cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3-TspE1 by gene-gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining.


CONCLUSIONS
The recombinant plasmid pcDNA3-TspE1 was successfully constructed and expressed in the BALB/c mice.
Cui
Jing
J
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhang
Hong-wei
HW
Wang
Zhong-quan
ZQ
Li
Yong-long
YL
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis / Q000235:genetics; D019470:Biolistics; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics / Q000276:immunology; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology; D010957:Plasmids / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017160:Trichinella spiralis / Q000276:immunology*; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000276:immunology
D000947:Antigens, Helminth; D004274:DNA, Recombinant; D019444:Vaccines, DNA