Expression of monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 in porcine circovirus 2-induced granulomatous inflammation.
The expression of monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1) in granulomatous inflammation of lymph nodes was determined in 1-day-old piglets inoculated intranasally with porcine circovirus 2 (PCV2). The methods used were reverse transcription-polymerase chain reaction (RT-PCR), granulomatous lesion scoring, and in-situ hybridization. Maximum MCP-1 expression and MIP-1 expression were observed at 17 and 21 days post-inoculation (dpi), respectively. Granulomatous lesions of maximum severity were observed somewhat later (28 dpi). A close cell to cell relation between PCV2 and the two cytokines in serial sections from lymph nodes suggested that their induction was a direct response to PCV2. The results provide evidence of the ability of MCP-1 and MIP-1 to induce granulomatous inflammation in vivo.
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 141-742, Seoul, South Korea.
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Kim
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Chae
D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D054407:Chemokine CCL4; D018173:Circoviridae Infections / Q000276:immunology / Q000473:pathology*; D017925:Circovirus / Q000276:immunology; D006099:Granuloma / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D017403:In Situ Hybridization; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D018932:Chemokine CCL2; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins