Mycobacterium parmense sp. nov.
The isolation and identification of a novel, slow-growing, scotochromogenic, mycobacterial species is reported. A strain, designated MUP 1182T, was isolated from a cervical lymph node of a 3-year-old child. MUP 1182T is alcohol- and acid-fast, with a lipid pattern that is consistent with those of species that belong to the genus Mycobacterium. It grows slowly at 25-37 degrees C, but does not grow at 42 degrees C. The isolate was revealed to be biochemically distinct from previously described mycobacterial species: it has urease and Tween hydrolysis activities and lacks nitrate reductase, 3-day arylsulfatase and beta-glucosidase activities. Comparative 16S rDNA sequencing showed that isolate MUP 1182T represents a novel, slow-growing species that is related closely to Mycobacterium lentiflavum and Mycobacterium simiae. On the basis of these findings, the name Mycobacterium parmense sp. nov. is proposed, with MUP 1182T (=CIP 107385T=DSM 44553T) as the type strain.
Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma, 43100 Parma, Italy.
Franco
F
Fanti
Laboratorio di Microbiologia e Virologia, Ospedale di Careggi, 50139 Florence, Italy.
Enrico
E
Tortoli
Mayo Clinic and Mayo Foundation, Division of Clinical Microbiology, Rochester, MN 55905, USA.
Leslie
L
Hall
Mayo Clinic and Mayo Foundation, Division of Clinical Microbiology, Rochester, MN 55905, USA.
Glenn D
GD
Roberts
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 38124 Braunschweig, Germany.
Reiner M
RM
Kroppenstedt
Dipartimento Materno-Infantile, Reparto di Pediatria e Oncoematologia, Azienda Ospedaliera di Parma, 43100 Parma, Italy.
Icilio
I
Dodi
Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma, 43100 Parma, Italy.
Stefania
S
Conti
Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma, 43100 Parma, Italy.
Luciano
L
Polonelli
Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma, 43100 Parma, Italy.
Carlo
C
Chezzi
D001192:Arylsulfatases / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D020459:Genes, rRNA; D006801:Humans; D008055:Lipids / Q000032:analysis; D008198:Lymph Nodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D010802:Phylogeny; D011136:Polysorbates / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013194:Staining and Labeling; D013696:Temperature; D014510:Urease / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D011136:Polysorbates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D001192:Arylsulfatases; D001617:beta-Glucosidase; D014510:Urease