Preparation and characterization of two LysB29 specifically labelled fluorescent derivatives of human insulin.
The preparation and characterization of two novel LysB29 selectively labelled fluorescent derivatives of human insulin are described. Two probes were chosen: 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) and 7-methoxycoumarin-4-acetic acid (MCA), which have a relatively small, compact structure and are able to react with amino groups to form highly fluorescent derivatives. The combination of solid phase peptide synthesis and enzymatic semisynthesis was chosen for preparation of these fluorescent derivatives. Using two different protocols of solid-phase peptide synthesis, two fluorescent octapeptides were prepared corresponding to the position B23-B30 of human insulin, each with a different fluorescent label, NBD or MCA, on the epsilon-amino group of lysine. Then, the fluorescent octapeptides were coupled to desoctapeptide-(B23-B30)-insulin by a trypsin catalysed reaction. The receptor binding affinities of two novel fluorescent derivatives of human insulin with NBD and MCA (HI-NBD and HI-MCA) were determined on rat adipose tissue plasma membranes. Both fluorescent insulins, HI-NBD and HI-MCA, had only slightly reduced binding affinity and will be used for studying the interaction of insulin with its receptor.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic.
Alice
A
Ciencialová
Lenka
L
Záková
Jiri
J
Jirácek
Jana
J
Barthová
Tomislav
T
Barth
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D007328:Insulin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism
D005456:Fluorescent Dyes; D007328:Insulin; D011972:Receptor, Insulin; D008239:Lysine