Application of moderate hydrostatic pressure induces unit-cell changes in rhombohedral insulin.
2Co(2+)-insulin crystals were subjected to hydrostatic pressures of up to 30 bar in a nitrogen gas cell. Changes in the diffraction pattern occurred at pressures as low as 5 bar. Analysis with standard image-processing software showed unit-cell dimension changes resulting in reductions in volume of up to 2.6%.
Nicholson
J
J
Biophysics Department, School of Biomolecular Sciences, Liverpool John Moores University, England.
Körber
F
F
Lambert
S
S