Impact of sewage sludges on Medicago truncatula symbiotic proteome.
The effects of sewage sludges were investigated on the symbiotic interactions between the model plant Medicago truncatula and the arbuscular mycorrhizal fungus Glomus mosseae or the rhizobial bacteria Sinorhizobium meliloti. By comparison to a control sludge showing positive effects on plant growth and root symbioses, sludges enriched with polycylic aromatic hydrocarbons or heavy metals were deleterious. Symbiosis-related proteins were detected and identified by two-dimensional electrophoresis and matrix-assisted laser desorption ionization mass spectrometry, and image analysis was used to study the effects of sewage sludges on M. truncatula symbiotic proteome.
UMR 1088 INRA/CNRS 5184/UB (Plante-Microbe-Environnement), INRA-CMSE, BP 86510, 21065 Dijon cedex, France.
Gwénaëlle
G
Bestel-Corre
Silvio
S
Gianinazzi
Eliane
E
Dumas-Gaudot
D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D029902:Medicago / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D038821:Mycorrhizae / Q000254:growth & development / Q000378:metabolism; D010449:Peptide Mapping; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D012722:Sewage / Q000032:analysis*; D016962:Sinorhizobium meliloti / Q000254:growth & development / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis
D010940:Plant Proteins; D020543:Proteome; D012722:Sewage