Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus.
The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 A. They belong to the space group P2(1)2(1)2(1) with cell dimensions a = 45.4, b = 85.7, c = 143.1 A with a single molecule of laccase in the asymmetric unit.
Department of Chemistry, University of York, Heslington, England.
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V
Ducros
G J
GJ
Davies
D M
DM
Lawson
K S
KS
Wilson
S H
SH
Brown
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P
Østergaard
A H
AH
Pedersen
P
P
Schneider
D S
DS
Yaver
A M
AM
Brzozowski