The bisphosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats.
Many materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. We analyzed the quantity of new bone formed in vivo around calcium-immobilized titanium implants with surfaces modified using pamidronate (PAM), a nitrogen-containing bisphosphonate (N-BP), implants of pure titanium, and titanium implants immobilized with calcium ions. New bone formation was visualized using fluorescent labeling (calcein blue and alizarin complexone) with intravenous injection at 1 and 3 weeks after implantation. After 4 weeks, undecalcified sections were prepared, and new bone formation around the implants was examined by morphometry using confocal laser scanning microscopy images. After 1 week, more new bone formed around the PAM-immobilized implant than around the calcium-immobilized and pure titanium implants. This was also seen with the new bone formation after 3 weeks. After 4 weeks, significantly more new bones were formed around the BP-immobilized implant than around the calcium ion-implanted and pure titanium implants. The new N-BP-modified titanium surface stimulates new bone formation around the implant, which might contribute to the success of implant therapy.
Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science, 3-1-1 Maidashi Fukuoka 812-8582, Japan.
Hiroshi
H
Kajiwara
Takayoshi
T
Yamaza
Masao
M
Yoshinari
Tetsuya
T
Goto
Shinji
S
Iyama
Ikiru
I
Atsuta
Mizuho A
MA
Kido
Teruo
T
Tanaka
D000818:Animals; D001862:Bone Resorption / Q000517:prevention & control; D002118:Calcium; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004164:Diphosphonates / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D018613:Microscopy, Confocal; D016348:Osseointegration / Q000187:drug effects*; D000077268:Pamidronate; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000187:drug effects / Q000601:surgery / Q000648:ultrastructure; D014025:Titanium
D020099:Coated Materials, Biocompatible; D004164:Diphosphonates; D014025:Titanium; D000077268:Pamidronate; D002118:Calcium