[Expression of the mouse serotonin receptor (5HT1c) cDNA in cultured insect cells].
Baculovirus-mediated cloning and expression of the mouse serotonin receptor (5HT1c) cDNA in insect cells was proposed to obtain an alternative to an oocyte-based system, which is commonly employed in electrophysiological studies of ionic channels. A recombinant bacmid was constructed, and the 5HT1c cDNA transferred into the AcNPV genome to yield a recombinant baculovirus. Infected inset Sf9 cells produced recombinant 5HT1c.
S N
SN
Belzhelarskaia
F
F
Sattov
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D051379:Mice; D008969:Molecular Sequence Data; D011985:Receptors, Serotonin / Q000235:genetics*; D018411:Spodoptera / Q000166:cytology / Q000235:genetics*
D018076:DNA, Complementary; D011985:Receptors, Serotonin; C112812:serotonin 1C receptor