Association between paraoxonase-1 activity and lipid peroxidation indicator levels in people living in the Antalya region with angiographically documented coronary artery disease.
BACKGROUND
Paraoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated enzyme capable of hydrolyzing lipid peroxides. Thus, PON1 plays a preventing role in atherosclerosis by protecting against lipid peroxidation.


OBJECTIVE
The incidence of coronary artery disease (CAD) is high in the Turkish population, and many risk factors have been studied as determinants of CAD. In Turkish people living in the Antalya region, we aimed to determine serum PON1 activity and its relation to lipoproteins and lipid peroxidation markers.


METHODS
We measured the activity of serum PON1 together with concentrations of a variety of lipid constituents--total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), HDL cholesterol (HDL-C), triglycerides (TG), apolipoprotein (apo) A-I, apoB, and lipid peroxidation indicators (conjugated diene [CD] and thiobarbituric acid-reactive substances [TBARS])--in 108 patients with CAD and 64 healthy subjects (controls).


RESULTS
We found that the PON1 activity was significantly reduced in patients with CAD (222.37 +/- 11.31 IU/l) compared with controls (331.75 +/- 20.98 IU/l). These patients had significantly lower HDL-C, PON1/HDL-C, apoA-I, PON1/ApoA-I, and ApoA-I/ApoB, and higher LDL-C, TC/HDL-C, LDL-C/HDL-C, apoB, CD and TBARS than did controls. Total cholesterol and apoA-I concentrations were significantly higher in women than in men in both groups. After multiple logistic regression analysis, TBARS (odds ratio [OR] 568.87; p = 0.000), age (OR 1.10; p = 0.000), gender (OR 4.58; p = 0.008), apoA-I/apoB (OR 0.046; p = 0.003), and PON1/apoA-I (OR 0.58; p = 0.007) were independently indicative of the presence of CAD.


CONCLUSIONS
This is the first report of decreased serum PON1 activity and increased lipid peroxidation indicators (CD and TBARS) of patients with CAD living in Antalya, Turkey. Our results indicate that TBARS levels, age, gender, apoA-1/ApoB, and PON1/apoA-I ratios are important markers of CAD.
Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Ayşe Yeşim
AY
Göçmen
Saadet
S
Gümüşlü
Ender
E
Semiz
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000378:metabolism; D001055:Apolipoproteins B / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D015243:Cholesterol, VLDL / Q000378:metabolism; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000503:physiopathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D013223:Statistics as Topic; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014421:Turkey / Q000453:epidemiology
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D043303:Aryldialkylphosphatase