A powerful selection assay for mixture libraries of DNA alkylating agents.
A simple and powerful selection assay that permits the separation (rpHPLC), quantitation (ELSD), and identification (ESI-MS) of thermally released adenine adducts derived from duocarmycin analogues is detailed that can establish the most effective DNA alkylating agents in synthetic combinatorial mixtures.
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Young-Wan
YW
Ham
Dale L
DL
Boger
D000477:Alkylating Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000478:Alkylation / Q000187:drug effects; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D000080890:Duocarmycins; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology; D011760:Pyrrolidinones / Q000737:chemistry / Q000494:pharmacology
D000477:Alkylating Agents; D000080890:Duocarmycins; D007211:Indoles; D011758:Pyrroles; D011760:Pyrrolidinones; C066098:duocarmycin SA; D004247:DNA; C058289:duocarmycin A