Crystallization of the OP-G2 Fab fragment: a fibrinogen mimic with specificity for the platelet glycoprotein IIb/IIIa.
The OP-G2 monoclonal antibody binds to the platelet integrin, gpIIb/IIIa, in a mode that mimics fibrinogen binding. The specificity of this antibody is mediated by the third complementarity-determining region (CDR3) loop of the immunoglobulin heavy chain which contains a sequence (RYD) related to the RGD recognition sequence of fibrinogen. The OP-G2 Fab fragment has been crystallized by vapor diffusion from solutions containing polyethylene glycol and imidazole malate (pH 5.6). The crystals belong to space group P2(1)2(1)2 with a = 93.1, b = 83.8 and c = 53.7 A. One Fab molecule is present in the asymmetric unit. A complete data set to 2.0 A resolution has been collected.
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Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037, USA.
Williamson
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