Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b.
Methylosinus trichosporium OB3b oxidized methane to methanol in the presence of a high concentration of Cu2+. Further oxidation of methanol to formaldehyde was prevented by adding 200 mM NaCl which acted as a methanol dehydrogenase H inhibitor. The bacterium, 0.6 mg dry cell ml(-1), in methane/air (1:4, v/v) at 25 degrees C in 12.9 mM phosphate buffer (pH 7) containing 20 mM sodium formate and 200 mM NaCl accumulated 7.7 mM methanol over 36 h.
Department of Biomaterials, Chosun University, Gwangju 501-759, Korea.
Sang Gui
SG
Lee
Jae Hwan
JH
Goo
Hee Gon
HG
Kim
Jeong-Il
JI
Oh
Young Min
YM
Kim
Si Wouk
SW
Kim
D018929:Cell Culture Techniques / Q000379:methods*; D003300:Copper / Q000494:pharmacology; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D020602:Methylosinus trichosporium / Q000254:growth & development / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride; D003300:Copper; D010088:Oxidoreductases; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane; D000432:Methanol