Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase.
Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.
Shigechi
Hisayori
H
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 6547-8501, Japan.
Koh
Jun
J
Fujita
Yasuya
Y
Matsumoto
Takeshi
T
Bito
Yohei
Y
Ueda
Mitsuyoshi
M
Satoh
Eiichi
E
Fukuda
Hideki
H
Kondo
Akihiko
A
D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D010957:Plasmids; D011994:Recombinant Proteins; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013213:Starch / Q000378:metabolism*; D016985:Streptococcus bovis / Q000201:enzymology / Q000235:genetics; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D011994:Recombinant Proteins; D000431:Ethanol; D013213:Starch; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase