Application of derivative spectrophotometry to simultaneous determination of indomethacin and 5-methoxy-2-methyl-3-indoleacetic acid in metindol injections.
Derivative spectrophotometry was employed to develop a rapid and accurate method for simultaneous determination of indomethacin and 5-methoxy-2-methyl-3-indoleacetic acid as its possible impurity in Metindol injections. At the selected wavelengths, 233.04 and 284.65 nm, no interference between the components determined was observed. Under the established experimental conditions, recoveries of the particular components were from 96.14 to 98.17%. Linearity was maintained over a broad range of concentrations, from 11.88 x 10(-3) to 35.64 x 10(-3) mg/mL for indomethacin and 0.4 to 1.2 mg/mL for 5-methoxy-2-methyl-3-indoleacetic acid. The limit of detection was found to be 6.0 x 10(-3) mg/mL for indomethacin and 0.04 x 10(-3) mg/mL for 5-methoxy-2-methyl-3-indoleacetic acid. The limits of quantitation were found to be 10.0 x 10(-3) mg/mL and 0.20 x 10(-3) mg/mL, respectively.
Jagiellonian University, Collegium Medicum, Department of Inorganic and Analytical Chemistry, 30-688 Kraków, ul. Medyczna 9, Poland.
Mariusz
M
Stolarczyk
Jan
J
Krzek
Włodzimierz
W
Rzeszutko
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003720:Densitometry; D007210:Indoleacetic Acids / Q000032:analysis*; D007213:Indomethacin / Q000008:administration & dosage / Q000032:analysis*; D007267:Injections; D019999:Pharmaceutical Solutions; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007210:Indoleacetic Acids; D019999:Pharmaceutical Solutions; C030098:5-methoxy-methylindoleacetic acid; D007213:Indomethacin