Protection of pigs from swine dysentery by vaccination with recombinant BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae.
Swine dysentery (SD) is an important endemic infection in many piggeries, and control can be problematic. In this study the efficacy of BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae, was evaluated as an SD vaccine. Non-lipidated BmpB was expressed in Escherichia coli as a histidine-tagged protein (His6-BmpB), or as an 8 kDa carboxy-terminal portion fused to maltose-binding protein (MBP-BmpB-F604). The purified proteins were emulsified with oil-based adjuvants for intramuscular (im) administrations. In experiment 1, 20 weaner pigs were vaccinated im with 1 mg of His6-BmpB. After 3 weeks, 10 received 1 mg of the protein orally (im/oral), and 10 received 1 mg im (im/im). Ten acted as unvaccinated controls. In experiment 2, 12 pigs were vaccinated im with 1 mg of His6-BmpB, and 12 with 1 mg of MBP-BmpB-F604. Three weeks later, each was given 1 mg of the same protein orally. Twelve pigs acted as unvaccinated controls. All pigs were challenged orally with B. hyodysenteriae 2 weeks after their second vaccination. In both experiments, all pigs vaccinated with His6-BmpB developed serum antibodies to BmpB, and oral administration provided boosting of im-induced serum antibody titres. In experiment 1, seven non-vaccinated control pigs developed dysentery and severe colitis. Three pigs vaccinated im/oral developed diarrhoea; two had severe colitis and one had mild lesions. Four pigs vaccinated im/im developed diarrhoea; one had severe colitis and the others had mild lesions. In experiment 2, six control pigs developed SD with severe colitis. Two His6-BmpB vaccinated pigs developed SD with mild colitis. Nine pigs vaccinated with MBP-BmpB-F604 developed SD and severe colitis. Overall, 50-70% of controls and 17-40% of His6-BmpB vaccinated pigs developed disease. Vaccination with MBP-BmpB-F604 did not induce serum titres against BmpB, nor confer protection. The incidence of disease for the three His6-BmpB vaccinated groups was significantly less (P = 0.047) than for the control groups, with a approximately 50% reduction. BmpB appears to have potential as an SD vaccine component.
La
Tom
T
School of Veterinary and Biomedical Sciences, Division of Veterinary and Biomedical Sciences, Murdoch University, WA 6150, Australia.
Phillips
Nyree D
ND
Reichel
Michael P
MP
Hampson
David J
DJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D003106:Colon / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004403:Dysentery / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D058619:Maltose-Binding Proteins; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013144:Spirochaetales / Q000235:genetics / Q000276:immunology*; D013145:Spirochaetales Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D022223:Vaccines, Subunit / Q000276:immunology / Q000627:therapeutic use
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C415945:BmpB protein, Brachyspira hyodysenteriae; D002352:Carrier Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins; D058619:Maltose-Binding Proteins; D011994:Recombinant Proteins; D022223:Vaccines, Subunit