A RAPD marker associated with B chromosomes in Partamona helleri (Hymenoptera, Apidae).
The hymenopteran Partamona helleri is found in southwestern Brazil in the Mata Atlântica from the north of the state of Santa Catarina until the south of Bahia. This work shows that P. helleri can carry up to four B chromosomes per individual. In order to obtain more information about P. helleri B chromosomes, the RAPD technique was used to detect DNA fragments associated with these chromosomes. The results showed that the RAPD technique is useful to detect specific sequences associated with B chromosomes. One RAPD marker was identified, cloned and used as probe in a DNA blot analysis. This RAPD marker hybridized with sequences present only in individuals containing B chromosomes.
Tosta
V C
VC
Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa-MG, Brazil. vandertosta@yahoo.com.br
Fernandes-Salomão
T M
TM
Tavares
M G
MG
Pompolo
S G
SG
Barros
E G
EG
Campos
L A O
LA
D000818:Animals; D001516:Bees / Q000235:genetics*; D001938:Brazil; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D020732:Cytogenetic Analysis / Q000379:methods; D004247:DNA / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D008677:Metaphase / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D005819:Genetic Markers; D004247:DNA