Antisera to polycyclic aromatic hydrocarbons and their application on the detection of chemical carcinogens in blood sera of oncological patients.
OBJECTIVE
To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure.


METHODS
Reactions of antibodies with haptens have been estimated by non-competitive and competitive ELISA with synthesized protein conjugates of PAHs as a coating antigene.


RESULTS
All the model rabbit antisera have been stated to react with anthracene, chrysene, pyrene, benzo(a)pyrene and benz(a)anthracene irrespective of the hapten used to immunize animals. The cross-reactivity of serum antibodies to all five PAHs has been found in blood samples of oncological patients.


CONCLUSIONS
It is recommended to use the conjugates of anthracene, chrysene and pyrene for detecting human antibodies to more dangerous environmental carcinogens--benzo(a)pyrene and benz(a)anthracene.
Kemerovo Scientific Centre, Siberian Branch of Russian Academy of Sciences, Kemerovo 650610, Russia. dci@kemtel.ru
Andrey N
AN
Glushkov
Tatiana P
TP
Anosova
Mikhail V
MV
Kostyanko
Mikhail P
MP
Anosov
Elena G
EG
Polenok
Sergey V
SV
Cherno
Stella A
SA
Mun
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000378:metabolism*; D002273:Carcinogens / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D007114:Immunization; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000941:Antigens; D002273:Carcinogens; D006241:Haptens; D007106:Immune Sera; D011084:Polycyclic Aromatic Hydrocarbons; D012710:Serum Albumin, Bovine