Elastic light scattering from single cells: orientational dynamics in optical trap.
Light-scattering diagrams (phase functions) from single living cells and beads suspended in an optical trap were recorded with 30-ms time resolution. The intensity of the scattered light was recorded over an angular range of 0.5-179.5 degrees using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed.
Genoptix, San Diego, California, USA.
Dakota
D
Watson
Norbert
N
Hagen
Jonathan
J
Diver
Philippe
P
Marchand
Mirianas
M
Chachisvilis
D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured; D004548:Elasticity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008545:Melanoma / Q000473:pathology*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation; D019047:Phantoms, Imaging; D010812:Physical Stimulation / Q000295:instrumentation / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity