Monosialoganglioside increases catalase activity in cerebral cortex of rats.
Monosialoganglioside (GM1) is a neuroprotective agent that has been reported to scavenge free radicals generated during reperfusion and to protect receptors and enzymes from oxidative damage. However, only a few studies have attempted to investigate the effects of GM1 on enzymatic antioxidant defenses of the brain. In the present study, we evaluate the effects of the systemic administration of GM1 on the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and on spontaneous chemiluminescence and total radical-trapping potential (TRAP) in cerebral cortex of rats ex vivo. The effects of GM1 on CAT activity and spontaneous chemiluminescence in vitro were also determined. Animals received two injections of GM1 (50 mg/kg, i.p.) or saline (0.85% NaCl, i.p.) spaced 24 h apart. Thirty minutes after the second injection the animals were sacrificed and enzyme activities and spontaneous chemiluminescence and TRAP were measured in cell-free homogenates. GM1 administration reduced spontaneous chemiluminescence and increased catalase activity ex vivo, but had no effect on TRAP, SOD or GSH-Px activities. GM1, at high concentrations, reduced CATactivity in vitro. We suggest that the antioxidant activity of GM1 ganglioside in the cerebral cortex may be due to an increased catalase activity.
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Michele Rechia
MR
Fighera
Juliana Sartori
JS
Bonini
Roberto
R
Frussa-Filho
Carlos Severo
CS
Dutra-Filho
Martine E Kienzle
ME
Hagen
Maribel Antonello
MA
Rubin
Carlos Fernando
CF
Mello
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology*; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019097:Spin Trapping; D013482:Superoxide Dismutase / Q000378:metabolism
D005677:G(M1) Ganglioside; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase