[Studies on random amplified polymorphic DNA fingerprinting of Cortex Magnoliae Officinalis].
OBJECTIVE
To identify Chinese traditional medicine (CTM) "Hou-pu" (Cortex Magnoliae Officinalis), its counterfeits and substitues.


METHODS
Total genomic DNA samples of ten plant species were amplified by randomly amplified polymorphic DNA (RAPD).


RESULTS
Ten samples were able to be distinguished through their amplified DNA banding patterns on the agarose gels after electrophoresis.


CONCLUSIONS
RAPD is able to identify "Hou-pu", its counterfeits and substitutes quickly and truly, which is also quite valuable for correctly introducing plant.
Liu
Wensheng
W
Guangdong Provincial People's Hospital, Guangzhou 510080.
Zhu
Jianming
J
He
Bin
B
Su
Yingjuan
Y
D016000:Cluster Analysis; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination; D031566:Magnolia / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D018744:DNA, Plant