Signal peptide of potato PinII enhances the expression of Cry1Ac in transgenic tobacco.
The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.
State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, Beijing 100094, China.
Yun-Jun
YJ
Liu
Yuan
Y
Yuan
Jun
J
Zheng
Ya-Zhong
YZ
Tao
Zhi-Gang
ZG
Dong
Jian-Hua
JH
Wang
Guo-Ying
GY
Wang
D000595:Amino Acid Sequence; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D004731:Endotoxins / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics; D006460:Hemolysin Proteins; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics; D014026:Nicotiana / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018744:DNA, Plant; D004731:Endotoxins; D006460:Hemolysin Proteins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C034104:proteinase inhibitor II protein, plant; D049452:Green Fluorescent Proteins