D-003 and warfarin interaction on the bleeding time and venous thrombosis experimentally induced in rats.
D-003 is a mixture of higher aliphatic primary acids isolated and purified from sugarcane wax, the main component of which is octacosanoic acid. D-003 exhibits a cholesterol-lowering effect as well as antiplatelet and antithrombotic effects in experimental models. Warfarin is a coumarin derivative with anticoagulant activity that acts as a vitamin K antagonist. Since in clinical practice warfarin and D-003 could be administered together, the objective of this study was to evaluate the effects of the simultaneous administration of both drugs on the bleeding time and the venous thrombosis experimentally induced in rats. The combined therapy of minimally effective doses of D-003 and warfarin produced an antithrombotic effect significantly higher than those produced by each monotherapy. Likewise, the prolongation of bleeding time induced by warfarin was increased by the simultaneous administration with D-003, showing a synergistic effect between both drugs.
Arruzazabala
María Lourdes
ML
Center of Natural Products, National Center for Scientific Research, Ave. 25 and 158, PO Box 6880, Cubanacán, Havana, Cuba. cpn@cnic.edu.cu
Molina
Vivian
V
Carbajal
Daisy
D
Más
Rosa
R
D000818:Animals; D001760:Bleeding Time; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005227:Fatty Acids / Q000008:administration & dosage*; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020246:Venous Thrombosis / Q000188:drug therapy*; D014859:Warfarin / Q000008:administration & dosage*
C418063:D-003 sugarcane wax acid mixture; D005227:Fatty Acids; D005343:Fibrinolytic Agents; D014859:Warfarin