Cholesterol synthesis and de novo lipogenesis in premature infants determined by mass isotopomer distribution analysis.
Premature infants change from placental supply of mainly carbohydrates to an enteral supply of mainly lipids earlier in their development than term infants. The metabolic consequences hereof are not known but might have long-lasting health effects. In fact, knowledge of lipid metabolism in premature infants is very limited. We have quantified de novo lipogenesis and cholesterogenesis on d 3 of life in seven premature infants (birth weight, 1319 +/- 417 g; gestational age, 30 +/- 2 wk). For comparison, five healthy adult subjects were also studied. All subjects received a 12-h [1-(13)C] acetate infusion, followed by mass isotopomer distribution analysis (MIDA) on lipoprotein-palmitate and plasma unesterified cholesterol. The fraction of lipoprotein-palmitate synthesized at the end of the infusion period was 5.4 +/- 3.9% in infants, which was in the same range as found in adult subjects on a normal diet, suggesting that hepatic de novo lipogenesis is not a major contributor to fat accumulation in these premature neonates. The fractional contribution of newly synthesized cholesterol to plasma unesterified cholesterol was 7.4 +/- 1.3% after a 12-h infusion. The calculated rate of endogenous cholesterol synthesis was 31 +/- 7 mg/kg/d, a value approximately three times higher than that found in adult subjects (10 +/- 6 mg/kg/d). These results indicate that the cholesterol-synthesizing machinery is well developed in premature infants.
Groningen University Institute for Drug Exploration, Center for Liver, Digestive and Metabolic Diseases, Department of Pediatrics, University Hospital Groningen, 9700 RB Groningen, The Netherlands.
Lorraine N
LN
Renfurm
Robert H J
RH
Bandsma
Henkjan J
HJ
Verkade
Christiaan V
CV
Hulzebos
Theo
T
van Dijk
Theo
T
Boer
Frans
F
Stellaard
Folkert
F
Kuipers
Pieter J J
PJ
Sauer
D000085:Acetates / Q000493:pharmacokinetics; D000328:Adult; D002247:Carbon Isotopes; D002784:Cholesterol / Q000032:analysis / Q000096:biosynthesis*; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis; D014280:Triglycerides / Q000032:analysis / Q000096:biosynthesis
D000085:Acetates; D002247:Carbon Isotopes; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol