Human diffusely adhering Escherichia coli expressing Afa/Dr adhesins that use human CD55 (decay-accelerating factor) as a receptor does not bind the rodent and pig analogues of CD55.
Afa/Dr diffusely adhering Escherichia coli (DAEC) bacteria that are responsible for recurrent urinary tract and gastrointestinal infections recognized as a receptor the glycosylphosphatidylinositol (GPI)-anchored protein decay-accelerating factor (DAF; CD55) at the brush border of cultured human intestinal cells. Results show that Afa/Dr DAEC C1845 bacteria were poorly associated with the mucosa of the gastrointestinal tract of infected mice. We conducted experiments with Chinese hamster ovary (CHO) cells stably transfected with mouse (GPI or transmembrane forms), pig, or human CD55 or mouse Crry cDNAs or transfected with empty vector pDR2EF1 alpha. Recombinant E. coli AAEC185 bacteria expressing Dr or F1845 adhesins bound strongly to CHO cells expressing human CD55 but not to the CHO cells expressing mouse (transmembrane and GPI anchored), rat, or pig CD55 or mouse Crry. Positive clustering of CD55 around Dr-positive bacteria was observed in human CD55-expressing CHO cells but not around the rarely adhering Dr-positive bacteria randomly distributed at the cell surface of CHO cells expressing mouse, rat, or pig CD55.
Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie Paris XI, F-92296 ChAtenay-Malabry, France.
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D018830:Adhesins, Escherichia coli / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D018958:CD55 Antigens / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005856:Germ-Free Life; D006388:Hemagglutinins / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D051379:Mice; D051381:Rats; D013552:Swine
D018830:Adhesins, Escherichia coli; D018958:CD55 Antigens; C465564:Dr adhesin, E coli; D029968:Escherichia coli Proteins; D006388:Hemagglutinins