Combined enzymatic hydrolysis and HPAEC method for simultaneous analysis of galacturonic acid and neutral sugars of pectin.
Pectic substances are heteropolysaccharides from plant cell walls, which mainly consist of a homogalacturonan backbone of predominantly alpha-(1-->4) linked galacturonic acid (GalA) residues. This chain is interrupted by ramified rhamnogalacturonan regions with a certain amount of neutral sugars (rhamnose, arabinose, galactose, glucose, xylose and mannose) present as side-chains. The GalA residues can be methyl-esterified at the carboxyl group. Usually uronic acids of pectin are determined as anhydrogalacturonic acid by spectrophotometry, using the metahydrodiphenyl method (Thibault, 1979), while the monosaccharides are determined by High-Performance Liquid Chromatography (HPLC) after a methanolysis (Quemener and Thibault, 1990) or by Gaz Liquid Chromatography (GLC) as alditol acetates according to Blakeney et al. (1983). In this paper, a combined enzymatic hydrolysis and HPLC method is validated for simultaneous analysis of galacturonic acid and neutral sugars without any derivatisation.
Garna
H
H
Unité de Chimie Biologique Industrielle, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Deportes, 2 B-5030, Gembloux.
Mabon
N
N
Wathelet
B
B
Paquot
M
M
D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006603:Hexuronic Acids / Q000032:analysis* / Q000302:isolation & purification; D006868:Hydrolysis; D027845:Malus; D010368:Pectins / Q000737:chemistry / Q000378:metabolism*
D002241:Carbohydrates; D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins