Crystal structure of the bacterial nucleoside transporter Tsx.
Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.
Department of Cell Biology, Howard Hughes Medical Institute and Harvard Medical School, Boston, MA 02115, USA.
Jiqing
J
Ye
Bert
B
van den Berg
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D033703:Nucleoside Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D033703:Nucleoside Transport Proteins; D009705:Nucleosides; D011991:Receptors, Virus; C066743:Tsx protein, E coli