A combination of selective COX-2 inhibitors and hydrogen peroxide increase the reactive oxygen species formation in osteosarcoma cells after X-ray irradiation.
We investigated whether a combination of selective COX-2 inhibitors and hydrogen peroxide increases the effect of X-ray irradiation, with regard to reactive oxygen species (ROS) formation in an osteosarcoma cell line. COX-2 inhibitor did not induce ROS formation when combined with irradiation. A low dose concentration of COX-2 inhibitor in combination with hydrogen peroxide and irradiation did affect ROS formation in the intracellular compartment; however, this same combination of agents at high doses did not modulate the effect of irradiation. Therefore, low doses of COX-2 inhibitor and hydrogen peroxide together, in combination with irradiation, is a potentially useful alternative form of radiotherapy for apoptotic-resistant neoplasms such as osteosarcoma.
Department of Orthopaedics, Kochi Medical School, Kochi University, Oko-cho, Nankoku, Kochi 783-8505, Japan. takahast@kochi-ms.ac.jp
Toshiaki
T
Takahashi
Yasuhiro
Y
Ogawa
Toshihiro
T
Kobayashi
Hiroshi
H
Sonobe
Harumichi
H
Seguchi
Toshikazu
T
Tani
Shoji
S
Yoshida
D000068579:Celecoxib; D045744:Cell Line, Tumor; D003470:Culture Media; D016861:Cyclooxygenase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004347:Drug Interactions; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D011720:Pyrazoles / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000528:radiation effects; D013449:Sulfonamides / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D016861:Cyclooxygenase Inhibitors; D011720:Pyrazoles; D017382:Reactive Oxygen Species; D013449:Sulfonamides; D006861:Hydrogen Peroxide; D000068579:Celecoxib