On the slowing of S phase in response to DNA damage in fission yeast.
Eukaryotic cells slow their progression through S phase upon DNA damage. The mechanism that leads to this slowing is called the intra-S-phase checkpoint. Previous studies demonstrated that in the fission yeast Schizosaccharomyces pombe this checkpoint is mediated by a pathway that includes Rad3 (similar to human ATR and ATM) and Cds1 (similar to human Chk1 and Chk2). Here we present evidence that a major downstream target of this pathway is the cyclin-dependent kinase, Cdc2. We also present evidence suggesting that the intra-S-phase checkpoint makes a relatively minor contribution to the survival of cells with damaged DNA.
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Sanjay
S
Kumar
Joel A
JA
Huberman
D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics; D004249:DNA Damage; D005838:Genotype; D016296:Mutagenesis; D016196:S Phase; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*
D016203:CDC2 Protein Kinase