Mobile DNA elements in the gas vesicle gene cluster of the planktonic cyanobacteria Microcystis aeruginosa.
Insertion sequences (IS) have been characterized in Microcystis aeruginosa gas vesicle-deficient mutants. ISMae4, a homolog of the cyanobacterial IS702, belongs to the IS5 family, subgroup ISL2. ISMae2 and ISMae3 display typical IS features and express a transposase of the IS4 and IS1 family, respectively. ISMae1 exhibits a more complex genetic structure and harbours a degenerated transposase gene distantly related to IS1 elements. Hybridizations with IS-specific DNA probes suggest that transposition of ISMae2 and ISMae3 occurred by a replicative-type mechanism. To our knowledge this is the first report showing that IS1 elements can be mobile in cyanobacteria.
Unité des Cyanobactéries (URA-CNRS 2172), Département de Microbiologie fondamentale et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
Alyssa
A
Mlouka
Katia
K
Comte
Nicole
N
Tandeau de Marsac
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004261:DNA Replication; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D023061:Gene Order; D005798:Genes, Bacterial; D046931:Microcystis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D019895:Transposases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011506:Proteins; C038524:gas vesicle protein; D019895:Transposases