Structural and expression analysis of the porcine FUS2 gene.
The putative porcine tumor suppressor gene FUS2 or N-acetyltransferase (Nat6) assigned to SSC13q21 spans 864-basepairs (bp) of genomic DNA, consisting of a single exon encoding a protein of 288 amino acids (aa), with 73% identity to the human and 74% to the mouse protein. Similar to man and mouse, the gene possesses an N-acetyltransferase domain, but the cell attachment motif arginine-glycine-aspartate (RGD) is exclusively found in the pig gene. Expression studies of the gene in several organs by RT-amplification and by quantitative polymerase chain reaction (Q-PCR) showed that FUS2 is widely expressed in porcine tissues. A point mutation was detected at position 836 of the coding sequence (G to A) leading to an amino acid substitution from cystein (C) to tyrosine (Y) at position 278 of the protein. Genes of the tumor suppressor gene (TSG) cluster act together to suppress tumor growth through their functional activation of tumor suppressing pathways. Studies in humans have proven that mutations in N-acetyltransferase genes are associated with some kind of cancers. Knowledge of structure and function of the respective porcine genes and proteins is important. Pigs-in particular minipigs-will be the non-rodent biomodels for human oncology and cancer therapy in the future.
Gatphayak
Kesinee
K
Institute of Veterinary Medicine, University of Göttingen, Groner Landstrasse 2 37073 Göttingen, Germany.
Knorr
Christoph
C
Chen
Kefei
K
Brenig
Bertram
B
D000123:Acetyltransferases / Q000235:genetics*; D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes / Q000235:genetics; D016147:Genes, Tumor Suppressor; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D004247:DNA; D000123:Acetyltransferases; D001191:Arylamine N-Acetyltransferase