Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites.
A water sample from the North Sea was used to isolate the abundant heterotrophic bacteria that are able to grow on complex marine media. Isolation was by serial dilution and spread plating. Phylogenetic analysis of nearly complete 16S rRNA gene sequences revealed that one of the strains, HEL-45T, had 97.4% sequence similarity to Sulfitobacter mediterraneus and 96.5 % sequence similarity to Staleya guttiformis. Strain HEL-45T is a Gram-negative, non-motile rod and obligate aerobe and requires sodium and 1-7% sea salts for growth. It contains storage granules and does not produce bacteriochlorophyll. Optimal growth temperatures are 25-30 degrees C. The DNA base composition (G+C content) is 60.1 mol%. Strain HEL-45T has Q10 as the dominant respiratory quinone. The major polar lipids are phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl choline, phosphatidyl ethanolamine and an aminolipid. The fatty acids comprise 18 : 1omega7c, 18 : 0, 16 : 1omega7c, 16 : 0, 3-OH 10 : 0, 3-OH 12 : 1 (or 3-oxo 12 : 0) and traces of an 18 : 2 fatty acid. Among the hydroxylated fatty acids only 3-OH 12 : 1 (or 3-oxo 12 : 0) appears to be amide linked, whereas 3-OH 10 : 0 appears to be ester linked. The minor fatty acid components (between 1 and 7%) allow three subgroups to be distinguished in the Sulfitobacter/Staleya clade, placing HEL-45T into a separate lineage characterized by the presence of 3-OH 12 : 1 (or 3-oxo 12 : 0) and both ester- and amide-linked 16 : 1omega7c phospholipids. HEL-45T produces indole and derivatives thereof, several cyclic dipeptides and thryptanthrin. Phylogenetic analysis of 16S rRNA gene sequences and chemotaxonomic data support the description of a new genus and species, to include Oceanibulbus indolifex gen. nov., sp. nov., with the type strain HEL-45T (=DSM 14862T=NCIMB 13983T).
Wagner-Döbler
Irene
I
GBF - Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany.
Rheims
Holger
H
GBF - Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany.
Felske
Andreas
A
GBF - Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany.
El-Ghezal
Aymen
A
Technical University of Braunschweig, D-38106 Braunschweig, Germany.
Flade-Schröder
Dirk
D
Technical University of Braunschweig, D-38106 Braunschweig, Germany.
Laatsch
Hartmut
H
University of Göttingen, D-37077 Göttingen, Germany.
Lang
Siegmund
S
Technical University of Braunschweig, D-38106 Braunschweig, Germany.
Pukall
Rüdiger
R
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany.
Tindall
Brian J
BJ
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany.
D000220:Adaptation, Biological; D000332:Aerobiosis; D001429:Bacteriochlorophylls / Q000096:biosynthesis; D001482:Base Composition; D003594:Cytoplasmic Granules; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D007211:Indoles / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009068:Movement; D009659:North Sea; D009997:Osmotic Pressure; D010456:Peptides, Cyclic / Q000096:biosynthesis; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012965:Sodium Chloride; D013696:Temperature; D014871:Water Microbiology
D001429:Bacteriochlorophylls; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D007211:Indoles; D008055:Lipids; D010456:Peptides, Cyclic; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; C030374:indole; D005840:Gentian Violet