Morphogenesis and regionalization of the medaka embryonic brain.
We examined the morphogenesis and regionalization of the embryonic brain of an acanthopterygian teleost, medaka (Oryzias latipes), by in situ hybridization using 14 gene probes. We compared our results with previous studies in other vertebrates, particularly zebrafish, an ostariophysan teleost. During the early development of the medaka neural rod, three initial brain vesicles arose: the anterior brain vesicle, which later developed into the telencephalon and rostral diencephalon; the intermediate brain vesicle, which later developed into the caudal diencephalon, mesencephalon, and metencephalon; and the posterior brain vesicle, which later developed into the myelencephalon. In the late neural rod, the rostral brain bent ventrally and the axis of the brain had a marked curvature at the diencephalon. In the final stage of the neural rod, ventricles began to develop, transforming the neural rod into the neural tube. In situ hybridization revealed that the brain can be divided into three longitudinal zones (dorsal, intermediate, and ventral) and many transverse subdivisions, on the basis of molecular expression patterns. The telencephalon was subdivided into two transverse domains. Our results support the basic concept of neuromeric models, including the prosomeric model, which suggests the existence of a conserved organization of all vertebrate neural tubes. Our results also show that brain development in medaka differs from that reported in other vertebrates, including zebrafish, in gene-expression patterns in the telencephalon, in brain vesicle formation, and in developmental speed. Developmental and genetic programs for brain development may be somewhat different even among teleosts.
Kage
Takahiro
T
National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Takeda
Hiroyuki
H
Yasuda
Takako
T
Maruyama
Kouichi
K
Yamamoto
Naoyuki
N
Yoshimoto
Masami
M
Araki
Kazuo
K
Inohaya
Keiji
K
Okamoto
Hiroyuki
H
Yasumasu
Shigeki
S
Watanabe
Kaori
K
Ito
Hironobu
H
Ishikawa
Yuji
Y
D000818:Animals; D019521:Body Patterning / Q000235:genetics; D001921:Brain / Q000196:embryology*; D001931:Brain Mapping; D019070:Cell Lineage / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D017403:In Situ Hybridization; D038081:Organogenesis / Q000235:genetics / Q000502:physiology*; D009990:Oryzias / Q000196:embryology* / Q000235:genetics
D005819:Genetic Markers