Real-time quantitative assay of telomerase activity using the duplex scorpion primer.
A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R2 = 0.994). The requirement of post-PCR steps is thus obviated.
Huang
Yanping
Y
State Key Lab of Functional Polymer Materials for Adsorption and Separation, Chemical School, Nankai University, Tianjin 300071, PR China.
Kong
Deming
D
Yang
Yi
Y
Niu
Ruifang
R
Shen
Hanxi
H
Mi
Huaifeng
H
D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D004789:Enzyme Activation; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D019098:Telomerase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*
D014408:Biomarkers, Tumor; D017931:DNA Primers; D019098:Telomerase