Identification of post-mortem cerebrospinal fluid proteins as potential biomarkers of ischemia and neurodegeneration.
Only few biological markers are currently available for the routine diagnosis of brain damage-related disorders including cerebrovascular, dementia, and other neurodegenerative diseases. In this study, post-mortem cerebrospinal fluid samples were used as a model of massive brain insult to identify new markers potentially relevant for neurodegeneration. The protein pattern of this sample was compared to the one of cerebrospinal fluid from healthy subjects by two-dimensional gel electrophoresis. Using gel imaging, N-terminal microsequencing, mass spectrometry, and immunodetection techniques, we identified 13 differentially expressed proteins. Most of these proteins have been previously reported to be somehow associated with brain destruction or with the molecular mechanisms underlying certain neurodegenerative conditions. These data indicate that the identified proteins indeed represent potential biomarkers of brain damage. We recently showed that H-FABP, a protein highly homologous to E-FABP and A-FABP identified in this study, is a potential marker of Creutzfeldt-Jakob disease and stroke.
Lescuyer
Pierre
P
Biomedical Proteomics Research Group, Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland. pierre.lescuyer@sim.hcuge.ch
Allard
Laure
L
Zimmermann-Ivol
Catherine G
CG
Burgess
Jennifer A
JA
Hughes-Frutiger
Séverine
S
Burkhard
Pierre R
PR
Sanchez
Jean-Charles
JC
Hochstrasser
Denis F
DF
D001344:Autopsy; D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D007511:Ischemia / Q000503:physiopathology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019636:Neurodegenerative Diseases / Q000503:physiopathology*; D010449:Peptide Mapping; D013129:Spinal Puncture
D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins