Crystallization and preliminary X-ray analysis of a cohesin domain of the cellulosome from Clostridium thermocellum.
Recombinant cohesin-2, a unique type of protein-recognition domain from the cellulosome of Clostridium thermocellum, has been crystallized by the hanging-drop vapor-diffusion method. The crystals are monoclinic, space group C2 with unit-cell dimensions a = 79.91, b = 47.86, c = 51.13 A, beta = 126.77 degrees. There is most likely to be one molecule per asymmetric unit, corresponding to a packing density of 2.16 A(3) Da(-1). The crystals diffract to beyond 2.3 A on a conventional laboratory rotating-anode source.
Shimon
L J
LJ
Department of Chemical Services, The Weizmann Institute of Science, Rehovot, Israel. cvlinda@weismann.ac.il
Frolow
F
F
Yaron
S
S
Bayer
E A
EA
Lamed
R
R
Morag
E
E
Shoham
Y
Y