Determination of liquiritigenin and isoliquiritigenin in Glycyrrhiza uralensis and its medicinal preparations by capillary electrophoresis with electrochemical detection.
A simple, reliable, reproducible and sensitive method, based on capillary electrophoresis with electrochemical detection (ED), for the determination of liquiritigenin and isoliquiritigenin in Glycyrrhiza uralensis and its medicinal preparations was described. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits good responses at + 1000 mV (versus SCE) for the two analytes. Under the optimum conditions, the analytes were base-line separated within 8 min, and excellent linearity was obtained in the concentration range from 5.0 x 10(-4) to 1.0 x 10(-6) mol/l. The detection limit (S/N = 3) was 4.7 x 10(-7) and 2.9 x 10(-7) mol/l for liquiritigenin and isoliquiritigenin, respectively. This work provides a useful method for the analysis of traditional Chinese medicines.
School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China. yuhuacao@yahoo.com.cn
Yuhua
Y
Cao
Yun
Y
Wang
Chao
C
Ji
Jiannong
J
Ye
D002599:Chalcone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D047188:Chalcones; D004339:Drug Compounding; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D006035:Glycyrrhiza / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D013607:Tablets
D047188:Chalcones; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; D013607:Tablets; D002599:Chalcone; C040920:isoliquiritigenin; C083152:liquiritigenin