Empirical modeling of batch fermentation kinetics for poly(glutamic acid) production and other microbial biopolymers.
An empirical kinetic model is proposed for the batch production of poly(glutamic acid) from Bacillus subtilis IFO 3335. In addition, the proposed model was used to fit the kinetic data of poly(glutamic acid) production from other bacterial strains using different media, as well as kinetic data from different strains for the production of the exocellular biopolymers dextran, hyaluronic acid, xanthan, alginate, and the endocellular biopolymer polyhydroxybutyrate. The empirical model treats the biopolymer as a component of the biomass and fits the experimental biomass data using a sigmoidal relationship that includes the maximum specific growth rate, mu(max), and the substrate saturation parameter, K(S). An empirical parameter, the relative coefficient (r), quantifies, in relative terms, the degree of nongrowth-associated biopolymer formation.
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada, N6A 5B9.
Andrew
A
Richard
Argyrios
A
Margaritis
D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D001704:Biopolymers / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D005285:Fermentation / Q000502:physiology; D007700:Kinetics; D008954:Models, Biological; D011099:Polyglutamic Acid / Q000096:biosynthesis*; D013045:Species Specificity
D001426:Bacterial Proteins; D001704:Biopolymers; D011099:Polyglutamic Acid