In situ analyses of genome instability in breast cancer.
Transition through telomere crisis is thought to be a crucial event in the development of most breast carcinomas. Our goal in this study was to determine where this occurs in the context of histologically defined breast cancer progression. To this end, we assessed genome instability (using fluorescence in situ hybridization) and other features associated with telomere crisis in normal ductal epithelium, usual ductal hyperplasia, ductal carcinoma in situ and invasive cancer. We modeled this process in vitro by measuring these same features in human mammary epithelial cell cultures during ZNF217-mediated transition through telomere crisis and immortalization. Taken together, the data suggest that transition through telomere crisis and immortalization in breast cancer occurs during progression from usual ductal hyperplasia to ductal carcinoma in situ.
Chin
Koei
K
Department of Laboratory Medicine and Comprehensive Cancer Center, University of California San Francisco, California, USA.
de Solorzano
Carlos Ortiz
CO
Knowles
David
D
Jones
Arthur
A
Chou
William
W
Rodriguez
Enrique Garcia
EG
Kuo
Wen-Lin
WL
Ljung
Britt-Marie
BM
Chew
Karen
K
Myambo
Kenneth
K
Miranda
Monica
M
Krig
Sheryl
S
Garbe
James
J
Stampfer
Martha
M
Yaswen
Paul
P
Gray
Joe W
JW
Lockett
Stephen J
SJ
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000473:pathology; D043171:Chromosomal Instability; D018450:Disease Progression; D006801:Humans; D006965:Hyperplasia / Q000235:genetics / Q000473:pathology; D017403:In Situ Hybridization; D016615:Telomere / Q000000981:diagnostic imaging*; D014407:Tumor Cells, Cultured; D014463:Ultrasonography