Differentiation of the major Listeria monocytogenes serovars by multiplex PCR.
A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. The PCR test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 Listeria strains.
Laboratoire des Listeria-Centre National de Référence des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France. mdoumith@pasteur.fr
Michel
M
Doumith
Carmen
C
Buchrieser
Philippe
P
Glaser
Christine
C
Jacquet
Paul
P
Martin
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005516:Food Microbiology; D006801:Humans; D008087:Listeria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial