Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana.
Despite the ubiquitous distribution of tRNA-related short interspersed elements (SINEs) in eukaryotic species, very little is known about the synthesis and processing of their RNAs. In this work, we have characterized in detail the different RNA populations resulting from the expression of a tRNA-related SINE S1 founder copy in Arabidopsis thaliana. The main population is composed of poly(A)-ending (pa) SINE RNAs, while two minor populations correspond to full-length (fl) or poly(A) minus [small cytoplasmic (sc)] SINE RNAs. Part of the poly(A) minus RNAs is modified by 3'-terminal addition of C or CA nucleotides. All three RNA populations accumulate in the cytoplasm. Using a mutagenesis approach, we show that the poly(A) region and the 3' end unique region, present at the founder locus, are both important for the maturation and the steady-state accumulation of the different S1 RNA populations. The observation that primary SINE transcripts can be post-transcriptionally processed in vivo into a poly(A)-ending species introduces the possibility that this paRNA is used as a retroposition intermediate.
CNRS UMR 6547 BIOMOVE and GDR 2157, Université Blaise Pascal Clermont-Ferrand II, 63177 Aubière Cedex, France.
Thierry
T
Pélissier
Cécile
C
Bousquet-Antonelli
Laurence
L
Lavie
Jean-Marc
JM
Deragon
D020413:3' Untranslated Regions; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D003593:Cytoplasm / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D026723:Polyadenylation; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D038621:Regulatory Sequences, Ribonucleic Acid; D020086:Short Interspersed Nucleotide Elements; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; D018749:RNA, Plant; D038621:Regulatory Sequences, Ribonucleic Acid; D012343:RNA, Transfer