[Isolation of cell membranes from Saccharomyces cerevisiae for evaluation of the protein composition].
We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts, containing the complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isolation of cell membranes. The membrane fraction obtained by this method was suitable for studies of protein composition of these structures by means of two-dimensional electrophoresis.
Aliverdieva
D A
DA
Pricaspian Institute for Bioresource, Dagestan Research Center, Russian Academy of Sciences, Makhachkala, 367025 Russia.
Malygin
A G
AG
Lagutina
L S
LS
Shol'ts
K F
KF
D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
D029701:Saccharomyces cerevisiae Proteins