Application of SEC-ICP-MS for comparative analyses of metal-containing species in cancerous and healthy human thyroid samples.
Size exclusion chromatography (SEC) was coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for speciation study of trace metals in cancerous thyroid tissues in comparison to healthy thyroids aimed to estimation of changes in metalloprotein speciation in pathological tissue. The study showed a presence of species binding Cu, Zn, Cd and Pb in healthy thyroid tissue with a good reproducibility of chromatographic results, whereas the same species could not be detected in cancerous tissues. Thus, remarkable differences with respect to metal-binding species were revealed between healthy and pathological thyroid samples, pointing out a completely different distribution of trace metals in cancerous tissues. The metal-binding species could not be identified in the frame of this work because of a lack of appropriate standards. Nevertheless, the results obtained confirm the suitability of SEC-ICP-MS for monitoring of changes in trace metal distribution in cancerous tissue and will help to better understand the role of metal-containing species in thyroid pathology.
Institute of Inorganic Chemistry and Analytical Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55099 Mainz, Germany.
Sergei F
SF
Boulyga
Valeria
V
Loreti
Jörg
J
Bettmer
Klaus G
KG
Heumann
D002850:Chromatography, Gel / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods; D008670:Metals / Q000032:analysis*; D013961:Thyroid Gland / Q000737:chemistry*; D013964:Thyroid Neoplasms / Q000737:chemistry*; D014131:Trace Elements / Q000032:analysis*
D008670:Metals; D014131:Trace Elements