Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America.
Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, beta-tubulin, and translation elongation factor-1alpha. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.
Department of Wood Science, Chair Forest Products Biotechnology, University of British Columbia, 4036-2424 Main Mall, Vancouver, BC, Canada V6T 1Z4.
Young Woon
YW
Lim
Sepideh Massoumi
SM
Alamouti
Jae-Jin
JJ
Kim
Sangwon
S
Lee
Colette
C
Breuil
D000199:Actins / Q000235:genetics; D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification; D017124:Conserved Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009656:North America; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
D000199:Actins; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D014404:Tubulin