Effect of cefazolin loaded bone matrix gelatin on repairing large segmental bone defects and preventing infection.
OBJECTIVE
To explore the possibility of repairing long segmental bone defects and preventing infection with cefazolin loaded bone matrix gelatin (C-BMG).


METHODS
C-BMG was made from putting cefazolin into BMG by vacuum absorption and lyophilization techniques. The sustaining period of effective drug concentration in vitro and in vivo was detected. The time of inhibiting bacteria, and the drug concentration in local tissues (bone and muscle) and plasma after implantation of C-BMG were examined by high performance liquid chromatography.


RESULTS
The effective inhibition time to staphylococcus aureus of C-BMG was 22 days in vitro; while 14 days in vivo. The cefazolin concentration in local tissues was higher in early stage, and later it kept a stable and low drug release. C-BMG showed an excellent ability to repair segmental long bone defects.


CONCLUSIONS
C-BMG can gradually release cefazolin with effective drug concentration and has excellent ability to repair segmental bone defects. It can be used to repair segmental long bone defects and prevent infection after operation.
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. youhongbo001@sina.com
Hong-bo
HB
You
An-min
AM
Chen
D000818:Animals; D001857:Bone Matrix; D018786:Bone Substitutes / Q000627:therapeutic use; D002437:Cefazolin / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005780:Gelatin; D008826:Microbial Sensitivity Tests; D010012:Osteogenesis; D019736:Prostheses and Implants; D011817:Rabbits; D011885:Radius Fractures / Q000601:surgery*; D013211:Staphylococcus aureus / Q000187:drug effects; D013530:Surgical Wound Infection / Q000517:prevention & control*
D018786:Bone Substitutes; D002511:Cephalosporins; D005780:Gelatin; D002437:Cefazolin