[Inhibitory effect of zinc on beta-hexosaminidase release from RBL-2H3 cells by synthetic chemicals].
The effects of foods and chemicals related to food hygiene on degranulation were evaluated using a method for assaying the enzyme activity of beta-hexosaminidase as an index of chemical mediator release from RBL-2H3 cells in vitro. Using a previously developed assay system, we had found a large number of inhibitors and promoters of degranulation of RBL-2H3 cells. In the present study, we examined the inhibitory effect of zinc chloride on the degranulation (beta-hexosaminidase release) from RBL-2H3 cells with or without antigen in the presence of the degranulation-promotive chemicals, namely, 4 food additives, 7 pesticides and 2 veterinary drugs. These promotive chemicals were classified into two types on the basis of inhibitory profile by zinc chloride: 1) those which showed marked degranulation-inhibitory action when the cells were stimulated with antigen, such as butylhydroxyanisole, dibutylhydroxytoluene, EPN, cis- and trans-permethrin, prothiofos, pyridaben, terbufos, 2) those which showed marked degranulation-inhibitory action whether the cells were stimulated with antigen or not, such as butyl p-hydroxybenzoate, o-phenylphenol, bitertanol, salinomycin. In conclusion, zinc had a dramatic inhibitory effect on enhanced degranulation induced by synthetic chemicals in vitro.
Tanaka
Yukio
Y
Osaka Prefectural Institute of Public Health, 1-3-69, Nakamichi, Higashinari-ku, Osaka 537-0025, Japan.
Taguchi
Shuzo
S
Yoshida
Seisaku
S
Hori
Shinjiro
S
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology*; D005503:Food Additives / Q000494:pharmacology*; D006589:Hexestrol / Q000494:pharmacology; D010575:Pesticides / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology; D051381:Rats; D017967:Zinc Compounds / Q000494:pharmacology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*; D000095662:Polyether Polyketides
D002712:Chlorides; D005503:Food Additives; D006589:Hexestrol; D010575:Pesticides; D011714:Pyrans; D017967:Zinc Compounds; D001619:beta-N-Acetylhexosaminidases; C010327:salinomycin; D000095662:Polyether Polyketides; C016837:zinc chloride