Combined enzymatic hydrolysis and HPAEC method for simultaneous analysis of galacturonic acid and neutral sugars of pectin.
Pectic substances are heteropolysaccharides from plant cell walls, which mainly consist of a homogalacturonan backbone of predominantly alpha-(1-->4) linked galacturonic acid (GalA) residues. This chain is interrupted by ramified rhamnogalacturonan regions with a certain amount of neutral sugars (rhamnose, arabinose, galactose, glucose, xylose and mannose) present as side-chains. The GalA residues can be methyl-esterified at the carboxyl group. Usually uronic acids of pectin are determined as anhydrogalacturonic acid by spectrophotometry, using the metahydrodiphenyl method (Thibault, 1979), while the monosaccharides are determined by High-Performance Liquid Chromatography (HPLC) after a methanolysis (Quemener and Thibault, 1990) or by Gaz Liquid Chromatography (GLC) as alditol acetates according to Blakeney et al. (1983). In this paper, a combined enzymatic hydrolysis and HPLC method is validated for simultaneous analysis of galacturonic acid and neutral sugars without any derivatisation.
Unité de Chimie Biologique Industrielle, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Deportes, 2 B-5030, Gembloux.
H
H
Garna
N
N
Mabon
B
B
Wathelet
M
M
Paquot
D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006603:Hexuronic Acids / Q000032:analysis* / Q000302:isolation & purification; D006868:Hydrolysis; D027845:Malus; D010368:Pectins / Q000737:chemistry / Q000378:metabolism*
D002241:Carbohydrates; D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins