[Simultaneous determination of stevioside, rebaudioside A and glycyrrhizic acid in foods by HPLC].
A method for the simultaneous determination of stevioside (Stev), rebaudioside A (RebA) and glycyrrhizic acid (GA) in foods was developed. These sweeteners were extracted from foods, except for dried fishes and shellfishes, by dialysis against Tris-HCl buffer (pH 9.0). Dried fishes and shellfishes were extracted with Tris-HCl buffer--methanol (2:8). The extracts were cleaned up with an Oasis MAX cartridge. The cartridge was washed with 0.05 mol/L sodium acetate (pH 4.0)--methanol (19:1), and the three sweeteners were eluted with 0.1 mol/L phosphoric acid--acetonitrile (1:1). Stev, RebA and GA in the eluate were chromatographed on a Develosil RPAQUEOUS-AR-5 (4.6 mm i.d. x 250 mm) column with 0.02 mol/L phosphoric acid-acetonitrile--methanol (90:55:5) as a mobile phase and monitored at 210 nm for Stev and RebA, and at 254 nm for GA. The recoveries of Stev, RebA and GA from 8 kinds of foods spiked at the level of 0.1 g/kg were 81.7-101%, 81.5-100% and 78.6-95.0%, respectively. The determination limits were 0.01 g/kg in samples.
Tama Branch Institute, Tokyo Metropolitan Institute of Public Health, 3-16-25, Shibasaki-cho, Tachikawa, Tokyo 190-0023, Japan.
Narue
N
Sakamaki
Hiroko
H
Matsumoto
Kayo
K
Hagino
Mitsuo
M
Nakazato
Kazuo
K
Yasuda
D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D045786:Diterpenes, Kaurane; D005504:Food Analysis / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D019695:Glycyrrhizic Acid / Q000032:analysis*; D013549:Sweetening Agents / Q000032:analysis*; D013729:Terpenes / Q000032:analysis*
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005960:Glucosides; D013549:Sweetening Agents; D013729:Terpenes; C012043:stevioside; D019695:Glycyrrhizic Acid; C025474:rebaudioside A