Electrocatalytic detection of thiols using an edge plane pyrolytic graphite electrode.
The first example of using an edge plane pyrolytic graphite electrode in electroanalysis is reported as the determination of homocysteine, N-acetylcysteine, cysteine and glutathione is studied. The response of the electrode in the direct oxidation of thiol moieties is explored and found to be electrocatalytic producing a reduction in the overpotential while having enhanced signal-to-noise characteristics compared to glassy carbon and basal plane pyrolytic graphite electrodes. The effectiveness of the methodology is examined in the determination of cysteine species in a growth tissue media that contains a high number of common biological interferences. The advantageous properties of this electrode for thiol determination lie in its excellent catalytic activity, sensitivity and simplicity.
Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, UKOX1 3QZ.
Ryan R
RR
Moore
Craig E
CE
Banks
Richard G
RG
Compton