Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens.
The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.
Department of Pediatrics, Baylor College of Meidcine, Houston, TX 77030, USA.
Andreea C
AC
Cazacu
Gail J
GJ
Demmler
Mark A
MA
Neuman
Betty A
BA
Forbes
Sooyoung
S
Chung
Jewel
J
Greer
Ana E
AE
Alvarez
Robin
R
Williams
Nadine Y
NY
Bartholoma
D002845:Chromatography / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009980:Influenza A virus / Q000145:classification / Q000302:isolation & purification*; D009981:Influenza B virus / Q000145:classification / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D007753:Laboratories / Q000592:standards; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012137:Respiratory System / Q000821:virology*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic