Solid phase synthesis of an amphiphilic peptide modified for immobilisation at the C-terminus.
The solid phase peptide synthesis (SPPS) of the amphiphilic peptide Ac-(Leu-Ala-Arg-Leu)(3)-linker, which is modified at the C-terminus with 1,8-diamino-3,6-dioxaoctane as linker moiety, has been investigated. Two different approaches that allow for the synthesis of C-terminally modified, side-chain protected peptides were examined. The solid phase peptide synthesis using aliphatic safety-catch resin followed by activation and aminolysis with the mono-Boc protected linker was compared with the synthesis on 1,8-diamino-3,6-dioxaoctane loaded 2-chlorotrityl resin.
Wilking
Sven D
SD
Organic and Bioorganic Chemistry, Department of Chemistry, University of Bielefeld, PO Box 100131, D-33501 Bielefeld, Germany.
Sewald
Norbert
N
D000327:Adsorption; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis*; D044367:Phase Transition; D011485:Protein Binding; D012117:Resins, Synthetic / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D003432:Cross-Linking Reagents; D010455:Peptides; D012117:Resins, Synthetic; D013501:Surface-Active Agents