Peptides resulting from the pVP2 C-terminal processing are present in infectious pancreatic necrosis virus particles.
The capsid of birnaviruses contains two proteins, VP2 and VP3, which derive from the processing of a large polyprotein, NH(2)-pVP2-VP4-VP3-COOH. The proteolytic cascade involved in processing the polyprotein, and in the final maturation of pVP2 (the precursor of VP2), has recently been shown to generate VP2 and four structural peptides in infectious bursal disease virus and blotched snakehead virus. The presence of peptides in infectious pancreatic necrosis virus particles was investigated using mass spectrometry and N-terminal sequencing of virus particles. Three peptides deriving from the C terminus of pVP2 (residues 443-486, 487-495 and 496-508 of the polyprotein) and 14 additional peptides produced by further processing of peptides [443-486] and [496-508] were identified. These results indicate that the presence of several virus-encoded peptides in the virions is a hallmark of birnaviruses.
Galloux
Marie
M
Unité de Virologie et Immunologie Moléculaires, INRA, F-78350 Jouy-en-Josas, France.
Chevalier
Christophe
C
Unité de Virologie et Immunologie Moléculaires, INRA, F-78350 Jouy-en-Josas, France.
Henry
Celine
C
Unité de Biochimie et Structure des Protéines, INRA, F-78350 Jouy-en-Josas, France.
Huet
Jean-Claude
JC
Unité de Biochimie et Structure des Protéines, INRA, F-78350 Jouy-en-Josas, France.
Costa
Bruno Da
BD
Unité de Virologie et Immunologie Moléculaires, INRA, F-78350 Jouy-en-Josas, France.
Delmas
Bernard
B
Unité de Virologie et Immunologie Moléculaires, INRA, F-78350 Jouy-en-Josas, France.
D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000032:analysis*; D018061:Infectious pancreatic necrosis virus / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D014771:Virion / Q000737:chemistry*
D036022:Capsid Proteins; D010446:Peptide Fragments; D011498:Protein Precursors