Six genes strongly regulated by mercury in Pisum sativum roots.
Suppression subtractive hybridisation was used to isolate heavy metal-induced genes from Pisum sativum roots hydroponically exposed to 5 microM HgCl2 and 10 microM EDTA. Six genes were induced out of which one, PsHMIP6B, was novel. The other genes (PsSAMT, PsI2'H, PsNDA, PsAPSR, PsPOD) had not previously been isolated from pea and sequenced. All six genes were also induced after exposure to 5 microM HgCl2 in the absence of EDTA. The induction pattern was in some cases different for the two Hg species, demonstrating a quicker response to-free Hg2+ than Hg-EDTA. The stress-specificity of the gene regulation was investigated by hydroponically adding 5 microM Cd2+. Most Hg-induced cDNAs were also induced by Cd2+ but to a smaller extent than after Hg exposure. In addition, the gene expression was also probed for tissue specificity, which showed that all six genes were expressed in roots and not in leaves.
Department of Chemistry, Biochemistry and Biophysics, Göteborg University, P.O. Box 462, 405 30 Göteborg, Sweden.
Helena
H
Sävenstrand
Ake
A
Strid
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004492:Edetic Acid / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D008628:Mercury / Q000494:pharmacology*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D004492:Edetic Acid; D008628:Mercury