Structure of the O-polysaccharide of Proteus serogroup O34 containing 2-acetamido-2-deoxy-alpha-D-galactosyl phosphate.
On mild acid degradation of the lipopolysaccharide of Proteus vulgaris O34, strain CCUG 4669, the O-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. The resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text]The O-polysaccharide of Proteus mirabilis strain TG 276 was found to have the same structure and, based on the structural and serological data, this strain was proposed to be classified into the same Proteus serogroup O34.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
Andrei V
AV
Perepelov
Katarzyna
K
Kołodziejska
Anna N
AN
Kondakova
Marianna
M
Wykrota
Yuriy A
YA
Knirel
Zygmunt
Z
Sidorczyk
Antoni
A
Rozalski
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D005697:Galactosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry* / Q000302:isolation & purification; D011522:Protons; D012699:Serology; D013403:Sugar Phosphates / Q000737:chemistry*
D002247:Carbon Isotopes; D005697:Galactosides; D019081:O Antigens; D011522:Protons; D013403:Sugar Phosphates