Chitosan/gelatin composite microcarrier for hepatocyte culture.
Solid and porous chitosan/gelatin (CG) composite microcarriers were prepared by a water-in-oil emulsion process with additional freezing and lyophilization. Adult rat hepatocytes (10(6) cells ml(-1)) attached on CG microcarriers maintained at least 15 d of viability and differentiated functions. Over 15 d, unimmobilized hepatocytes released 1.34-fold less lactate dehydrogenase (LDH), and retained 1.63-, 1.51- and 1.28-fold higher albumin secretion, urea synthesis and 7-ethoxycoumarin deethylation activities, respectively, than those on collagen-coated microcarriers. The CG matrix is therefore a promising microcarrier for hepatocyte culture.
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Keguo
K
Li
Yun
Y
Wang
Zhenchuan
Z
Miao
Dayong
D
Xu
Yuefeng
Y
Tang
Meifu
M
Feng
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D048271:Chitosan / Q000737:chemistry*; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
D001672:Biocompatible Materials; D005780:Gelatin; D048271:Chitosan