Fermentation of sugar cane bagasse hemicellulose hydrolysate to L(+)-lactic acid by a thermotolerant acidophilic Bacillus sp.
Sugar cane bagasse hemicellulose, hydrolyzed by dilute H2SO4, supplemented with mineral salts and 0.5% corn steep liquor, was fermented to L(+)-lactic acid using a newly isolated strain of Bacillus sp. In batch fermentations at 50 degrees C and pH 5, over 5.5% (w/v) L(+)-lactic acid was produced (89% theoretical yield; 0.9 g lactate per g sugar) with an optical purity of 99.5%.
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA.
Milind
M
Patel
Mark
M
Ou
L O
LO
Ingram
K T
KT
Shanmugam
D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis*; D011134:Polysaccharides / Q000378:metabolism*; D031786:Saccharum / Q000378:metabolism* / Q000382:microbiology*; D013045:Species Specificity; D013696:Temperature
D011134:Polysaccharides; D019344:Lactic Acid; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse