Crystallization and preliminary X-ray analysis of human dUTPase.
Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P2(1)2(1)2(1) with cell dimensions a = 67.51, b = 68.26 and c = 91.00 A. The crystallographic asymmetric unit contains one trimer of identical subunits.
Larsson
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Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. gunilla.larsson@biokem.lu.se
Nyman
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Svensson
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