Use of low-molecular-weight polyethylene glycol in the crystallization of RNA oligomers.
We have crystallized a variety of RNA oligonucleotides in a form suitable for X-ray diffraction studies using polyethylene glycol with a low-molecular-weight distribution (PEG 400) as the precipitant. Crystallization experiments on a set of 26 RNA oligomers ranging from eight to 12 nucleotides in length resulted in eight diffraction-quality crystals. Of these eight RNA crystals, six utilized PEG 400 as the precipitating agent. We have also been able to obtain large single crystals of a DNA-RNA hybrid, transfer RNA (two different conditions) and a catalytic RNA from PEG 400 solutions. These results suggest that PEG 400 may be a generally useful alternative to 2-methyl-2,4-pentanediol (MPD) which has, thus far, been the most successful precipitant for DNA oligomers.
Structural Biology Division, Melvin Calvin Laboratory, University of California, Berkeley 94720, USA.
K J
KJ
Baeyens
J
J
Jancarik
S R
SR
Holbrook