Nicotinic acetylcholine receptor subtypes in nociceptive dorsal root ganglion neurons of the adult rat.
Stimulation of nicotinic acetylcholine receptors (nAChR) excites peripheral sensory nerve fibres, but also exert antinociceptive effects. The differences in these nAChR-mediated effects could be related to the expression of different nAChR subtypes located on nociceptive neurons. In the present study, we focused on the recently described alpha 10-nAChR subunit, and on alpha 4 and alpha 7 subunits, which are the most abundant subunits in the central nervous system. In nociceptive neurons from thoracic and lumbar dorsal root ganglia (DRG), nAChR subunits were found at transcriptional (RT-PCR), translational (immunohistochemistry) and functional levels. Cultured DRG neurons express mRNA for the subunits alpha 2-7 and alpha 10. The alpha-subunit proteins 4, 7 and 10 were colocalised in virtually all nociceptive neurons that were identified by immunoreactivity for the vanilloid receptor TRPV-1. These findings were corroborated by current recordings and calcium measurements, which revealed excitatory inward currents and calcium responses in capsaicin sensitive neurons.
Haberberger
Rainer Viktor
RV
Institute for Anatomy and Cell Biology Justus Liebig University, Aulweg 123, D-35385 Giessen, Germany. rainer.v.haberberger@anatomie.med.uni-giessen.de
Bernardini
Nadia
N
Kress
Michaela
M
Hartmann
Petra
P
Lips
Katrin Susanne
KS
Kummer
Wolfgang
W
D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology; D010146:Pain / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011978:Receptors, Nicotinic / Q000096:biosynthesis* / Q000145:classification
D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D009538:Nicotine