An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton.
A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C18 column (4.6 mm x 250 mm, 5 microm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. Absolute recoveries were > 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glanded Gossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton.
Cai
Yingfan
Y
College of Bioinformation, Chongqing University of Post and Telecom, Chongqing, People's Republic of China.
Zhang
Hong
H
Zeng
Yu
Y
Mo
Jianchuan
J
Bao
Jinku
J
Miao
Chen
C
Bai
Jie
J
Yan
Fang
F
Chen
Fang
F
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005838:Genotype; D003368:Gossypium / Q000235:genetics / Q000378:metabolism*; D006072:Gossypol / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results
D006072:Gossypol