An improved 'cold SSCP' method for the genotypic and subgenotypic characterization of Cryptosporidium.
A simple, non-isotopic PCR-based single-strand conformation polymorphism ('cold SSCP') method is described which allows the efficient detection of genetic variation among and within genotypes of Cryptosporidium parvum. This low cost approach has important advantages over other 'genotyping' methods and is applicable to a wide range of genetic loci and organisms.
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Vic. 3030, Australia. robinbg@unimelb.edu.au
Robin B
RB
Gasser
Youssef G
YG
Abs EL-Osta
Sandra
S
Prepens
Rachel M
RM
Chalmers
D000818:Animals; D003080:Cold Temperature; D003458:Cryptosporidium / Q000235:genetics*; D005838:Genotype; D018807:Polymorphism, Single-Stranded Conformational