Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides x nigra DN34).
A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001T, was isolated from internal poplar tissues (Populus deltoidesxnigra DN34) and identified as a member of the genus Methylobacterium. Phylogenetic analyses showed that strain BJ001T is related to Methylobacterium thiocyanatum, Methylobacterium extorquens, Methylobacterium zatmanii and Methylobacterium rhodesianum. However, strain BJ001T differed from these species in its carbon-source utilization pattern, particularly its use of methane as the sole source of carbon and energy, an ability that is shared with only one other member of the genus, Methylobacterium organophilum. In addition, strain BJ001T is the only member of the genus Methylobacterium to be described as an endophyte of poplar trees. On the basis of its physiological, genotypic and ecological properties, the isolate is proposed as a member of a novel species of the genus Methylobacterium, Methylobacterium populi sp. nov. (type strain, BJ001T=ATCC BAA-705T=NCIMB 13946T).
Department of Civil and Environmental Engineering, University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USA.
Benoit
B
Van Aken
Department of Microbiology, University of Iowa, 3-432 Bowen Science Building, Iowa City, IA 52242, USA.
Caroline M
CM
Peres
Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195, USA.
Sharon Lafferty
SL
Doty
Department of Civil and Environmental Engineering, University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USA.
Jong Moon
JM
Yoon
Department of Civil and Environmental Engineering, University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USA.
Jerald L
JL
Schnoor
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D020459:Genes, rRNA; D008697:Methane / Q000378:metabolism*; D020580:Methylobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D032107:Populus / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane