Preliminary crystallographic analysis of the ParE subunit of Escherichia coli topoisomerase IV.
The ParE subunit of Escherichia coli topoisomerase IV has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylyl-beta,gamma-imidodiphosphate (ADPNP). The crystals are of the orthorhombic space group, P2(1)2(1)2(1), with unit-cell dimensions a = 92.6, b = 119.1, c = 135.3 A. Data have been collected to 3.5 A resolution from frozen native crystals. Self-rotation function analysis of these data indicate the position of a molecular twofold axis. Higher resolution native data are being collected and a derivative search is underway.
Subramanya
H S
HS
Laboratory of Molecular Biophysics, University of Oxford, England.
Peng
H
H
Marians
K J
KJ
Wigley
D B
DB