Functional significance of S6K overexpression in meningioma progression.
One common genetic change in anaplastic meningiomas is amplification of chromosome 17q23 containing the S6 kinase (S6K) gene. We show, for the first time to our knowledge, increased S6K mRNA expression in anaplastic meningiomas compared with benign tumors. To evaluate S6K as a candidate meningioma progression gene, we generated IOMM-Lee human meningioma cell lines overexpressing S6K. Whereas no effect of S6K overexpression on meningioma cell growth, motility, or adhesion was observed in vitro, S6K overexpression resulted in increased tumor size in vivo. Collectively, these results suggest that S6K is functionally important for meningioma progression and may represent a target for future meningioma therapy.
Surace
Ezequiel I
EI
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Lusis
Eriks
E
Haipek
Carrie A
CA
Gutmann
David H
DH
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D018450:Disease Progression; D005786:Gene Expression Regulation; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019893:Ribosomal Protein S6 Kinases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D014162:Transfection / Q000379:methods
D012333:RNA, Messenger; D019893:Ribosomal Protein S6 Kinases