Crystallization and preliminary X-ray crystallographic studies of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli.
Crystals of 7alpha-hydroxysteroid dehydrogenase from E. coli, which is a tetramer in its active form, have been obtained by a hanging-drop vapor-diffusion method in the presence of the coenzyme NAD(+). Crystals as large as 0.25 x 0.25 x 0.75 mm could be grown within a month at pH 8.5 with polyethylene glycol as precipitating agent. Preliminary X-ray crystallographic analysis revealed that they belong to one of the enantiomorphic space groups P4(1)2(1)2 or P4(3)2(1)2 with dimensions a = b = 81.66 and c = 214.6 A, having two subunits in an asymmetric unit. The crystals diffract to at least 2.3 A resolution.
Department of BioEngineering, Nagaoka University of Technology, Niigata, Japan.
N
N
Tanaka
T
T
Nonaka
T
T
Yoshimoto
D
D
Tsuru
Y
Y
Mitsui