The potential of poly(N-isopropylacrylamide) (PNIPAM)-grafted hyaluronan and PNIPAM-grafted gelatin in the control of post-surgical tissue adhesions.
Poly(N-isopropylacrylamide)-grafted hyaluronan (PNIPAM-HA) and PNIPAM-grafted gelatin (PNIPAM-gelatin), which exhibit sol-to-gel transformation at physiological temperature, were applied as control of tissue adhesions: tissue adhesion prevention material and hemostatic aid, respectively. The rat cecum, which was abraded using surgical gauze, was coated with PNIPAM-HA-containing PBS (concentration: 0.5 w/v%). The coated solution was immediately converted to an opaque precipitate at body temperature, which weakly adhered to and covered the injured rat cecum. One week after coating, tissue adhesion between the PNIPAM-HA-treated cecum and adjacent tissues was significantly reduced as compared with that between non-treated tissue and adjacent tissues. On the other hand, the coating of bleeding spots of a canine liver with PNIPAM-gelatin-containing PBS (concentration: 20 w/v%) resulted in spontaneous gel formation on the tissues and subsequently suppressed bleeding. Although these thermoresponsive tissue adhesion prevention and hemostatic materials are still prototypes at this time, both thermoresponsive biomacromolecules bioconjugated with PNIPAM, PNIPAM-HA and PNIPAM-gelatin, may serve as a tissue adhesion prevention material and hemostatic aid, respectively.
Department of Bioengineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. ohya@ri.ncvc.go.jp
Shoji
S
Ohya
Hiromichi
H
Sonoda
Yasuhide
Y
Nakayama
Takehisa
T
Matsuda
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001831:Body Temperature; D002432:Cecum / Q000187:drug effects / Q000293:injuries*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D011232:Chemical Precipitation; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects; D005355:Fibrosis; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D006488:Hemostasis, Surgical; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D006358:Hot Temperature; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D020100:Hydrogels; D007813:Laparotomy; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control*; D014945:Wound Healing / Q000187:drug effects
D000180:Acrylic Resins; D006490:Hemostatics; D020100:Hydrogels; C052970:poly-N-isopropylacrylamide; D005780:Gelatin; D006820:Hyaluronic Acid