Proteome-level differences between auxinic-herbicide-susceptible and -resistant wild mustard (Sinapis arvensis L.).
To identify proteins that may be involved in mediating auxinic herbicide resistance (i.e., resistance to dicamba, picloram, 2,4-D), we compared the proteomes of an auxinic-herbicide-susceptible (S) and -resistant (R) wild mustard (Sinapis arvensis L.) biotype at different developmental stages. Using two-dimensional electrophoresis and mass spectrometry, we identified 11 seedling and leaf proteins that showed reproducible differences in expression between the S and the R wild mustard biotype following application of dicamba. Our proteome-level studies revealed the increased expression of the enzyme peptidylprolyl cis-trans isomerase (PPIase), which has recently been implicated in auxin signal transduction. Juglone, an inhibitor of PPIase, interfered with the normal ability of R seeds to germinate in the presence of dicamba, whereas S seeds did not germinate in the presence of dicamba or dicamba plus juglone. When R and S plants (3-4 leaf stage) were treated with dicamba, S showed typical auxinic herbicide effects (e.g., epinasty) whereas R did not. However, the concomitant application of dicamba and juglone to greenhouse-grown R plants produced morphological changes that were consistent with known auxinic-herbicide-induced symptoms. This is the first report suggesting the potential involvement of differential expression of PPIase in mediating auxinic herbicide resistance.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
William
W
Yajima
J Christopher
JC
Hall
Nat N V
NN
Kav
D003996:Dicamba / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D019696:Peptidylprolyl Isomerase / Q000235:genetics; D010846:Picloram / Q000494:pharmacology; D020543:Proteome / Q000235:genetics*; D031226:Sinapis / Q000187:drug effects* / Q000235:genetics*
D006540:Herbicides; D007210:Indoleacetic Acids; D020543:Proteome; D019696:Peptidylprolyl Isomerase; D010846:Picloram; D003996:Dicamba