Stereospecific effects of catechin isomers on insulin induced lipogenesis in 3T3-L1 cells.
We studied the influence of (+)- and (-)-catechin contained in green tea on insulin induced lipogenesis in 3T3-L1 cells. In 14 days of culture with insulin, the intracellular triglyceride concentration and the activities of glycerophosphate dehydrogenase, a marker of adipose conversion, increased. The addition of 0.02 mg/ml (+)-isomer stimulated the accumulation of triglyceride induced by insulin, but the addition of the same concentration of (-)-isomer inhibited lipogenesis. The activities of glycerophosphate dehydrogenase were changed by (+)- and (-)-catechin in the same direction as the corresponding changes in triglyceride accumulation. These data suggest a biological significance of catechins, with opposite effects on lipid metabolism depending on the isomer.
Department of Health and Nutrition School of Health and Human Life, Nagoya Bunri University, Inazawa, Japan.
Miyako
M
Mochizuki
Noboru
N
Hasegawa
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin; D007536:Isomerism; D051379:Mice; D008517:Phytotherapy; D013662:Tea; D014280:Triglycerides / Q000378:metabolism*
D000960:Hypolipidemic Agents; D007328:Insulin; D013662:Tea; D014280:Triglycerides; D002392:Catechin