Towards complete dephosphorylation and total conversion of phytates in poultry feeds.
The rate of phytate P removal from feed (level of dephosphorylation, DL) and the extent to which the molecule of phytic acid is deprived of phosphate moieties (conversion degree, CD) were studied in vitro and in a feeding trial with broilers fed corn-soybean diets. In the in vitro model, phytase A asymptotically increased DL and CD. Phytase B influenced DL only at low dosages of phytase A [0 or 250 phytase activity units (FTU)/kg], but it enhanced CD irrespective of phytase A activity. In the feeding trial, 3-phytase A and 6-phytase A (at 750 FTU/kg) exerted similar effects on broiler performance and similarly influenced bone mineralization, P retention, and Ca retention. Phytase B [6,400 acid phosphatase activity units (ACPU)/kg] enhanced feed intake, BW gain (BWG), toe ash, and P retention but not the retention of Ca. Myo-inositol fed at 0.1% significantly increased BWG, but it reduced P retention. Under conditions of a higher CD (excess of phytase B), 3-phytase A was more effective in enhancing performance than 6-phytase A, but it reduced Ca retention. Lower phytase B activities (0 to 3,200 ACPU/kg) with added 6-phytase A were more necessary for optimal growth of chickens than for enhanced P and Ca retention (4,800 to 6,400 ACPU/kg). The efficacy of both forms of phytase A and phytase B depended on the Ca level in feed. There is enough evidence to conclude that myo-inositol phosphates resulting from simultaneous action of 3-phytase A and phytase B affect bird physiology differently than intermediates accumulated by the action of 6-phytase A and phytase B.
University of Agriculture, Department of Food Biotechnology, 29-Listopada Ave. 46, 31-425 Kraków, Poland. kzyla@ar.krakow.pl
K
K
Zyła
M
M
Mika
B
B
Stodolak
A
A
Wikiera
J
J
Koreleski
S
S
Swiatkiewicz
D010832:6-Phytase / Q000378:metabolism / Q000494:pharmacology; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism / Q000493:pharmacokinetics; D002136:Calcium, Dietary / Q000008:administration & dosage; D002645:Chickens / Q000502:physiology*; D004032:Diet; D004435:Eating; D007294:Inositol / Q000008:administration & dosage; D009929:Organ Size; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism / Q000493:pharmacokinetics; D010833:Phytic Acid / Q000378:metabolism* / Q000493:pharmacokinetics; D013025:Glycine max; D015430:Weight Gain; D003313:Zea mays
D002136:Calcium, Dietary; D010710:Phosphates; D010758:Phosphorus; D007294:Inositol; D010833:Phytic Acid; D010832:6-Phytase; D002118:Calcium