Preventive effect of Thea sinensis melanin against acetaminophen-induced hepatic injury in mice.
The preventive effect of Thea sinensis melanin (TSM) against overdoses of N-acetyl-p-aminophenol (NAPAP) was studied on ICR mice. Animals were given 400 mg/kg intraperitoneally (i.p.) of NAPAP, and TSM was injected i.p. in doses 10-40 mg/kg 2 h before intoxication. The protective effects were evidenced by a complete blockage of the NAPAP-induced elevation of plasma alanine aminotransferase (ALT) activity, decreased concentration of thiobarbituric acid reactive substances (TBARS) to the control level, and a partial prevention of reduced glutathione (GSH) depletion in the liver tissue. Preadministration of TSM also caused restoration of superoxide dismutase (SOD) activity and resumed content of coenzymes Q9 and Q10. TSM by itself, however, did not affect the hepatic functional parameters, including serum ALT, TBARS, GSH, SOD, or coenzymes Q in the liver. Administration of TSM caused a dose-dependent inhibition of N-nitrosodimethylamine demethylase activity with ED50 of 15.8 mg/kg. Activities of ethoxyresorufin O-dealkylase and pentoxyresorufin O-alkylase isozymes were changed insignificantly. The immune suppressive effect of NAPAP on the in vivo antibody-forming cell responses was demonstrated using ICR-sensitized mice with sheep red blood cells. The joint effect of TSM and NAPAP indicated the capability of TSM to recover immunity of the animals to the level of intact mice. Results obtained demonstrate that TSM preadministration can prevent the multiple toxic effects of NAPAP.
Hung
Yao-Ching
YC
Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan.
Sava
Vasyl M
VM
Makan
Svetlana Yu
SY
Hong
Meng-Yen
MY
Huang
G Steven
GS
D000082:Acetaminophen / Q000008:administration & dosage / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury; D005978:Glutathione / Q000032:analysis; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D008543:Melanins / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D008543:Melanins; D017392:Thiobarbituric Acid Reactive Substances; D000082:Acetaminophen; D000410:Alanine Transaminase; D005978:Glutathione