Autofluorescence of transplantable hepatoma A22 (MH-A22): prospects of tumor tissue optical biopsy.
OBJECTIVE
Autofluorescence of experimental tumor (hepatoma A22 (MH-A22)) was employed to discriminate the optical differences between necrotic and non-necrotic tumor, hemorrhagic tumor and healthy tissue.


METHODS
The experiment was performed ex vivo using the transplantable tumor from the right haunch of hybrid mice (C57Bl/CBA). Blue LED light (lambda em=405 nm) was applied for autofluorescence excitation and fibre optics based spectrofluorimeter was used for spectra detection.


RESULTS
We observed that necrotic tumor tissue is characterized by the absence of endogenous porphyrins fluorescence, and registered spectra do not possess differences in the red spectral region (600-710 nm) in comparison with normalized autofluorescence spectra of muscle. Moreover, only certain segments of non-necrotic tumor bear the fluorescence of endogenous porphyrins.


CONCLUSIONS
Based on the experimental results, we suggest that the absence of long-waved fluorescence differences between necrotic tumor tissue and healthy tissue, e.g. muscle can impede the demarcation between healthy and tumor tissue. The uneven distribution of endogenous porphyrins in non-necrotic tumor tissue as well as the absence of endogenous porphyrins fluorescence in the small experimental tumors complicates the localization of cancerous tissue based on the autofluorescence registration.
Tamosiunas
Mindaugas
M
Vilnius University, Laser Research Center, Vilnius 10222, Lithuania. mindaugas.tamosiunas@ff.vu.lt
Makaryceva
Julija
J
Labanauskiene
Jurate
J
Bagdonas
Saulius
S
Aleksandraviciene
Ceslava
C
Didziapetriene
Janina
J
Griciute
Laima
L
Rotomskis
Ricardas
R
D000818:Animals; D001706:Biopsy; D002678:Chimera; D005260:Female; D005453:Fluorescence; D008114:Liver Neoplasms, Experimental / Q000175:diagnosis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009336:Necrosis; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000637:transplantation