Stage of differentiation, proliferative index of tumor cells and cytotoxic activity of peripheral blood lymphocytes in colorectal cancer patients.
OBJECTIVE
To evaluate prognostic value of activity of cytotoxic lymphocytes of peripheral blood (CTL) of patients with colorectal cancer (CRC).


METHODS
2-year survival of 30 patients with colorectal cancer stage II (T2-3N0-1M0G1-3) was investigated in relation with clinical data, cytotoxic activity of peripheral blood lymphocytes (by MTT-test) and immunohistochemical peculiarities of tumor cells using anti-Ki-67-MABs.


RESULTS
It was revealed that some factors had prognostic significance, namely regional lymph nodes involvement (r = -0.69), proliferative activity of tumor cells (Ki-67-positivity) (r = -0.67), restoration of CTL function after operation (r = 0.46), adjuvant chemotherapy (r = 0.41) and differentiation stage of tumor (r = 0.40). Negative correlation between CTL activity before operation and tumor proliferative activity also exists (r = -0.35).


CONCLUSIONS
the data point to the involvement of natural killer cells in the control of tumor growth.
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiology NAS of Ukraine, Kyiv, Ukraine. kmol@rql.net.ua
Tatyana D
TD
Paholyuk
Lubov M
LM
Zacharzeva
Konstantin V
KV
Koshel
Gennadiy P
GP
Oliynichenko
Ninel M
NM
Berezhnaya
D000230:Adenocarcinoma / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation; D002455:Cell Division; D017024:Chemotherapy, Adjuvant; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D019394:Ki-67 Antigen