In vitro maturation rates of canine oocytes from anoestrous bitches in simple media.
The meiotic competence of canine oocytes collected from anoestrous bitch ovaries and cultured for 72 h in different media was studied. The base culture medium was TCM 199 enriched with 10% fetal bovine serum (TCM); the effect of supplementation with EGF (50 ng x mL(-1)) or ITS (insulin: 10 microg x mL(-1); transferrin: 5.5 microg x mL(-1); selenium: 5 microg x mL(-1)) was also studied. TCM was also compared to a Synthetic Oviductal Fluid (SOF). All the media contained FSH (0.1 UI x mL(-1)), LH (10 UI x mL(-1)), 17beta-oestradiol (4 microg x mL(-1)) and kanamycin. Despite the anoestrous stage of the donor bitches, resumption of meiosis occurred in a high proportion of the oocytes, (mean value 77.3%). The number of oocytes showing the 'germinal vesicle breakdown' nuclear stage was not influenced by the type of the culture medium used. ITS had a positive effect on nuclear progression to later stages (from metaphase I to metaphase II); however, this effect was not statistically significant.
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Padua, Italy. ada.rota@unipd.it
Ada
A
Rota
Giorgia
G
Cabianca
D000780:Anestrus; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D012643:Selenium / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D014168:Transferrin; D004815:Epidermal Growth Factor; D012643:Selenium