Phylogeny of the family Pasteurellaceae based on rpoB sequences.
Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
Institute of Veterinary Bacteriology, University of Bern, CH-3012 Bern, Switzerland.
Bożena
B
Korczak
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark.
Henrik
H
Christensen
SmartGene GmbH, PSE-C EPFL-Ecublens, CH-1015 Lausanne, Switzerland.
Stefan
S
Emler
Institute of Veterinary Bacteriology, University of Bern, CH-3012 Bern, Switzerland.
Joachim
J
Frey
Institute of Veterinary Bacteriology, University of Bern, CH-3012 Bern, Switzerland.
Peter
P
Kuhnert
D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D015661:Pasteurellaceae / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit