Concanavalin A crystallized in complex with the trisaccharide 3,6-di-O-methyl-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside.
Concanavalin A was co-crystallized in two crystal forms with 3,6-di-O-methyl- (alpha-D-mannopyranosyl) alpha- D-mannopyranoside, which is primarily responsible for the high-affinity binding of N-linked carbohydrates to concanavalin A. Both crystal forms have space group P2(1) and contain a complete concanavalin A tetramer in the asymmetric unit. Form A was crystallized using polyethylene glycol methyl ether as the precipitant and has unit-cell dimensions a = 59.83, b = 64.84 and c = 125.92 A, beta = 93.87 degrees. Form B was obtained using phosphate as the precipitant and has unit-cell dimensions a = 81.94, b = 66.75 and c = 108.92 A, beta = 97.58 degrees. Form B was stable in the X-ray beam for several days and diffracted to 3.15 A resolution. Form A crystals could not withstand X-ray radiation at room temperature, but produced high-quality data under cryogenic conditions. The latter are suitable for a 2.3 A resolution structure determination by molecular replacement.
Laboratorium voor Ultrastructuur, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Sint-Genesius-Rode, Belgium. bouckaej@vub.ac.be
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Bouckaert
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Poortmans
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Wyns