Isolation and expression analysis of the testis-specific gene, human OPPO1.
OBJECTIVE
To investigate human spermatogenesis, we isolated human testis-specific genes.


METHODS
Using mouse amino acid sequences, we found the region including homology in amino acid level in the human genome sequences. The primers encompassing introns were made and RT-PCR and RACE were carried out. The resultant PCR products were sequenced.


RESULTS
The full-length cDNA of human OPPO1 was isolated. It encodes 257 amino acid residues. The expression of the human OPPO1 was predominantly in the testis. On the other hand, partial cDNAs of ZNF8, GR194, GR219, GR093, GR046, GR163, and GR200 were expressed in the various tissues.


CONCLUSIONS
Our data suggests that the human OPPO1 may play important roles in human spermatogenesis.
Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa, Japan. toshim@asahikawa-med.ac.jp
Toshinobu
T
Miyamoto
Kazuo
K
Sengoku
Hiroaki
H
Hayashi
Yoshihito
Y
Sasaki
Naoyuki
N
Takuma
Tsuyoshi
T
Yamashita
Mutsuo
M
Ishikawa
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D005786:Gene Expression Regulation; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013737:Testis / Q000378:metabolism / Q000502:physiology
D018076:DNA, Complementary; C485357:ODF4 protein, human; D029589:Seminal Plasma Proteins