Evaluation of Xenostrip-Tv, a rapid diagnostic test for Trichomonas vaginalis infection.
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet mount and PCR test with primers TVK3 and TVK7 and/or a positive PCR test confirmed by a second PCR assay with primers TVA5-1 and TVA6; 26 (6%) were positive by wet mount, and 36 (8.4%) were positive by Xenostrip-Tv test. Since the Xenostrip-Tv test is rapid and easy to perform and proved to be more sensitive than wet mount, it should be considered as an alternative to wet mount for point-of-care diagnosis of trichomoniasis, especially in settings where microscopy is impractical.
Sexually Transmitted Infections Branch, Division of Sexually Transmitted Diseases Prevention, NCHSTP, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS-G39, Atlanta, GA 30333, USA. Apillay@cdc.gov
A
A
Pillay
J
J
Lewis
R C
RC
Ballard
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014245:Trichomonas Infections / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000302:isolation & purification*
D017931:DNA Primers; D011934:Reagent Strips