[Experimental study of periosteal osteoblasts adhesion to artificial bone scaffolds based on rapid prototype].
OBJECTIVE
To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.


METHODS
Infant rat calvarias osteoblasts were isolated and expanded in vitro and the cells (2nd passage) were seeded onto scaffolds with porosity 80%, 90%, 95% at a density of 2.06 x 10(9)/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.


RESULTS
(1) The osteoblasts' adhesion amounts increased with culture time in three porosity group (P < 0.05), but the increase were different among three groups, 80% group was 0.35 x 10(5), 90% group was 2.84 x 10(5); (2) Through SEM observations, it showed that osteoblasts adhered to all scaffolds well.


CONCLUSIONS
The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasibility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.
Xian
Su-qin
SQ
Key. Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Chai
Feng
F
Zhao
Yi-min
YM
Wang
Zhong-yi
ZY
Liu
Xiao-fang
XF
Li
Tiao-cheng
TC
D000818:Animals; D018786:Bone Substitutes; D002448:Cell Adhesion; D002478:Cells, Cultured; D010006:Osteoblasts / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes