[Analytical aspects of the semiquantitative determination of urinary iodine using ferroin: value of rapid screening for iodine deficiency or excess].
Iodine is an essential element for thyroid hormone synthesis. Iodine disorders induced biological and/to clinical expression of thyroid dysfunction. Inappropriate iodine intake (by default or by excess) is worrying in terms of public health in France regarding the iodine deficiency and the frequency of iatrogen iodine overloads. Urinary iodine determination which generally implicates the use of a cerimetric method, is an useful tool to evaluate iodine intakes. In this study, we described the analytical aspects of a semiquantitative method of urinary iodine using a redox indicator, ferroin. This method allows the screening of iodine excess or deficiency in a short time (< 3 hours) with a good specificity and sensitivity. Since this assay does not require specific apparatus, it could be easily developed in clinical chemistry laboratories for the detection of inappropriate iodine intakes, and could be useful for prevention programs of iodine deficiency.
Service de biochimie C, Groupe hospitalier Pitié-Salpêtrière, 47-83 boulevard de l'Hôpital, 75013 Paris. emmanuelle.plantin-carrenard@psl.ap-hop-paris.fr
E
E
Plantin-Carrenard
F
F
Cattan
A
A
Aurengo
B
B
Dumérat
M-J
MJ
Foglietti
J-L
JL
Beaudeux
D015982:Bias; D003124:Colorimetry / Q000379:methods / Q000592:standards; D016002:Discriminant Analysis; D006801:Humans; D007202:Indicators and Reagents; D007455:Iodine / Q000172:deficiency / Q000506:poisoning / Q000652:urine; D008403:Mass Screening / Q000379:methods* / Q000592:standards; D010084:Oxidation-Reduction; D010618:Phenanthrolines; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013696:Temperature; D013861:Thiocyanates / Q000652:urine; D013997:Time Factors; D016482:Urinalysis / Q000379:methods* / Q000592:standards
D007202:Indicators and Reagents; D010618:Phenanthrolines; D013861:Thiocyanates; C001635:ferroin; D007455:Iodine