Detection of human papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to the hybrid capture II and MY-PCR assays and a two-step nested PCR assay.
The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.
Giovannelli
Lucia
L
Dipartimento di Igiene e Microbiologia, Università di Palermo, Italy.
Lama
Anna
A
Capra
Giuseppina
G
Giordano
Viviana
V
Aricò
Pietro
P
Ammatuna
Pietro
P
D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis
D004279:DNA, Viral