Protective effect of (+)-catechin against 5-fluorouracil-induced myelosuppression in mice.
The object of this study was to investigate the efficacy of (+)-catechin, which was isolated from Actinidia arguta Planch (Actinidiaceae), as a bone marrow cell proliferation-promoting compound against the hematotoxicity of 5-fluorouracil (5-FU) in mice. Intraperitoneally injected (+)-catechin (1 and 10 mg/kg per day) accelerated the recovery of the number of white blood cells (WBC) and platelets (PLT) but did not affect the number of circulating red blood cells (RBC). (+)-Catechin also augmented the number of myelocytes and splenocytes. Dual color flow cytometric analysis revealed that (+)-catechin reversed the reduction of the population of leukocytes (CD11b+ monocytes, Gr-1+ granulocytes and CD3+ T and CD45RA+ B lymphocytes) in whole blood, spleen and bone marrow caused by 5-FU. (+)-Catechin (1 and 10 mg/kg per day) showed remarkable recovery of Gr-1+ cells in all three types of tissues and of CD11b+ cells in the bone marrow cells. These findings suggest that (+)-catechin selectively enhances the recovery of the population of granulocytes reduced by 5-FU in mice.
Department of Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.
Fumihide
F
Takano
Tomoaki
T
Tanaka
Jiro
J
Aoi
Nobuo
N
Yahagi
Shinji
S
Fushiya
D000818:Animals; D001769:Blood / Q000187:drug effects*; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D002392:Catechin / Q000627:therapeutic use*; D005260:Female; D005434:Flow Cytometry; D005472:Fluorouracil / Q000037:antagonists & inhibitors* / Q000633:toxicity; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors* / Q000633:toxicity; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
D007166:Immunosuppressive Agents; D002392:Catechin; D005472:Fluorouracil