Fine localization of Nefl and Nef3 and its exclusion as candidate gene for lens rupture 2(lr2).
Cataract causing lr2 gene is found in the CXSD mouse, which is a recombinant inbred strain of BALB/c and STS mice. For the process of positional cloning of lr2, several candidate genes were selected in the middle region of chromosome 14, but most of them were excluded by combination of recombination and homozygosity mapping. Components of neurofilament proteins, neurofilament light polypeptide (Nefl) and neurofilament3 medium (Nef3), were linked to D14Mit87 which was not separated from the lr2 locus in the homozygosity mapping. When the expression levels of Nefl and Nef3 in eyes were compared in CXSD and BALB/c mice, there were no differences in expression levels. The cDNA sequences of the two genes from CXSD, BALB/c and STS mice were subsequently compared. Several nucleotide differences in cDNA sequences were detected between the mice strains but the majority of the changes were silent mutations that did not alter the amino acids. The sole amino acid difference, E567K in the glutamate rich region of Nfm, between BALB/c and CXSD was found to be a simple genetic polymorphism because the same substitution existed in STS, a non-cataract mouse strain. Therefore we excluded Nefl and Nef3 from the candidate genes for lr2 based on expression and mutation analyses.
Rhee
Sang Dal
SD
Korea Research Institute of Chemical Technology, Yusong-gu, Taejon.
Kim
Eunmin
E
Yoon
Sungjoo Kim
SK
Yang
Sung-Don
SD
Okumoto
Masaaki
M
Han
Sang-Seop
SS
Song
Chang-Woo
CW
D000818:Animals; D002386:Cataract / Q000235:genetics*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D016900:Neurofilament Proteins / Q000032:analysis*
D016900:Neurofilament Proteins; C044882:neurofilament protein L; C061195:neurofilament protein M