Heparin binding protein is increased in chronic leg ulcer fluid and released from granulocytes by secreted products of Pseudomonas aeruginosa.
Recently it was demonstrated by Gautam, et al. that release of neutrophil-borne heparin-binding protein (HBP), also known as CAP37/azurocidin, induced endothelial hyperpermeability and neutrophil efflux. Here, we show that chronic leg ulcer fluid, in contrast to wound fluid from acute wounds, contains highly increased levels of HBP. Immunohistochemistry demonstrated the presence of HBP in chronic ulcer tissues. Furthermore, secreted products of Pseudomonas aeruginosa were found to induce release of HBP from human neutrophils. Our data suggest a possible link between bacterial presence and HBP-release in chronic ulcers. Thus, targeting HBP offers an interesting option for reduction of endothelial barrier dysfunction in chronic ulcers.
Section for Dermatology, Department of Medical Microbiology, Dermatology and Infection, Lund University, Biomedical Center, Lund, Sweden.
Katarina
K
Lundqvist
Heiko
H
Herwald
Andreas
A
Sonesson
Artur
A
Schmidtchen
D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D007611:Aprotinin / Q000378:metabolism; D001706:Biopsy; D001798:Blood Proteins / Q000096:biosynthesis / Q000502:physiology*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis / Q000502:physiology*; D015331:Cohort Studies; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D007871:Leg Ulcer / Q000382:microbiology / Q000473:pathology*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D014945:Wound Healing
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D017077:Culture Media, Conditioned; D007611:Aprotinin