Binding interactions of Escherichia coli with globotetraosylceramide (globoside) using a surface plasmon resonance biosensor.
Surface plasmon resonance with an alkane L1 chip was used to investigate the binding of uropathogenic Escherichia coli, carrying adhesion receptors, to globotetraosylceramide (globoside; GbO4). The immobilization of globoside was reproducible and resulted in a stable globoside layer on the L1 chip. The data indicated that the globoside-immobilized L1 chip could be used for studying interactions with live or chemically fixed E. coli. The results indicated that the dissociation time was significantly reduced in glutaraldehyde-fixed E. coli as compared to living cells. Overall, the report demonstrates the significance of the L1 chip in terms of sensitivity, specificity, handling, and speed when studying globoside/E. coli interactions. This model may assist in screening for compounds that can inhibit the binding of uropathogenic E. coli to glycolipid ligands on target cells.
Pourshafie
Mohammad R
MR
Department of Chemistry and Biomedical Sciences, University of Kalmar, Norra vagen 49, Kalmar SE-391 82, Sweden.
Marklund
Britt-Inger
BI
Ohlson
Sten
S
D001422:Bacterial Adhesion / Q000502:physiology; D015374:Biosensing Techniques / Q000379:methods; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism*; D005915:Globosides / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D005915:Globosides; C024032:globotetraosylceramide