Increase of crustacean sensitivity to purified hepatotoxic cyanobacterial extracts by manipulation of experimental conditions.
Toxic cyanobacterial blooms are one of the most common consequences of water eutrophication. Microbiotests with crustaceans are not expensive and are easy to prepare for screening tests. They can be applied in the determination of bioactivity and interaction between toxic substances in water, including hepatotoxins. The principal aim of this study was to modify the standard conditions in the Thamnotoxkit F trade mark and Artoxkit M in order to increase crustacean sensitivity to purified cyanobacterial extracts containing microcystins. The results reported show that exposure time, higher temperature, and presence of DMSO can increase the sensitivity of microbiotests to microcystins. The best sensitivity with the Artemia salina test was achieved after a 48-h exposure at 25 degrees C. The tests using a 24-h exposure at 27 degrees C were the most sensitive for Thamnocephalus platyurus. The test without preincubation with DMSO provided the best correlation of microcystin concentration and LC(50) for Thamnocephalus platyurus and is recommended.
Department of Applied Ecology, University of Lodz, 12/16 Banacha Str, 90-237 Lodz, Poland.
Agata
A
Drobniewska
Małgorzata
M
Tarczyńska
Joanna
J
Mankiewicz
Tomasz
T
Jurczak
Maciej
M
Zalewski
D000818:Animals; D033202:Anostraca / Q000187:drug effects*; D001156:Artemia / Q000187:drug effects*; D001427:Bacterial Toxins / Q000633:toxicity*; D001681:Biological Assay / Q000379:methods; D000087522:Cyanobacteria Toxins; D004121:Dimethyl Sulfoxide; D005068:Eutrophication; D008387:Marine Toxins / Q000633:toxicity*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000633:toxicity*; D013696:Temperature; D013997:Time Factors; D018675:Toxicity Tests
D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D004121:Dimethyl Sulfoxide