Catalytic activity of zymomonas mobilis extracellular "levan-levansucrase" complex in sucrose medium.
The fructan biosynthesis by ethanol sedimented "levan-levansucrase" complex from Zymomonas mobilis fermentation broth as well as purified levansucrase was investigated. The fructooligosaccharide (FOS) producing activity of "levan-levamsucrase" sediment was investigated in 55% sucrose syrup at 45 degrees C. It was shown that FOS in the syrup were presented by 1-kestose, 6-kestose, neokestose and nystose. The increase of gluconic acid concentration was observed in the reaction mixture during the incubation suggesting about presence of glucose/fructose oxidoreductase in "levan-levansucrase" sediment. The influence of ethanol, glycerol and NaCl on levan and fructooligosaccharide formation by "levan-levansucrase" complex and purified levansucrase was studied and the changes in the ratio between different activities of levansucrase (sucrose hydrolysis, levan biosynthesis and FOS formation) were observed. Ethanol increases the FOS biosynthesis part in total activity of purified levansucrase. The technology of the production of prebiotics containing food product--fructan syrup by "levan-levansucrase " sediment as biocatalyst was developed.
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda boulevard 4., LV 1586, Riga, Latvia.
M
M
Bekers
D
D
Upite
E
E
Kaminska
J
J
Laukevics
R
R
Ionina
A
A
Vigants
D002384:Catalysis; D003470:Culture Media; D005110:Extracellular Space / Q000201:enzymology; D005630:Fructans / Q000378:metabolism; D006602:Hexosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013395:Sucrose; D017355:Zymomonas / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D005630:Fructans; D013395:Sucrose; C072599:levan; D006602:Hexosyltransferases; C021513:levansucrase