Quantification of Saccharomyces cerevisiae viability using BacLight.
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
Environmental Biotechnology Laboratory, Department of Civil Engineering The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Tong
T
Zhang
Herbert H P
HH
Fang
D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival / Q000502:physiology*; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011419:Propidium; D011933:Reagent Kits, Diagnostic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000302:isolation & purification / Q000502:physiology
D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; D049452:Green Fluorescent Proteins; D011419:Propidium