Validation of a real-time monitoring method for aniline in freshwater by high-performance liquid chromatography on porous graphitic carbon/electrospray ionization tandem mass spectrometry.
Aniline is an anthropogenic organic compound widely used in polymer, rubber, pharmaceutical and dye industries but also used in biodegradability assays of chemical compounds as a positive biodegradation standard. By the two approaches, the rapid determination of aniline is necessary because of the high toxicity of aniline on hemoglobin. A high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for the determination of aniline in water is described here. This method, using benzylamine as internal standard, was validated. No time-consuming sample preparation was needed. A rapid separation (7 min between two chromatographic runs) of aniline and benzylamine was performed on a Hypercarb porous graphitic carbon column using a gradient of methanol and 100 mM formic acid. The obtained limits of detection and quantification were 10 and 1 ng/mL, respectively. The response for aniline was quadratic. We show that this problem could be circumvented by showing that the [calculated concentration = f(introduced concentration)] function was linear. The linearity range was 10-1000 ng/mL. An example of an application consisting of an aniline 42-day degradation kinetic in water was demonstrated.
Delépée
Raphaël
R
Institut de Chimie Organique et Analytique (ICOA), UMR 6005, Université d'Orléans, rue de Chartres. BP 6759, 45067 Orléans cedex 2, France. Raphael.Delepee@univ-orleans.fr
Chaimbault
Patrick
P
Antignac
Jean-Philippe
JP
Lafosse
Michel
M
D000814:Aniline Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000814:Aniline Compounds; C023650:aniline