Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates.
A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.
National Veterinary Institute, Section of Feed and Food Microbiology, P.O. Box 8156 Dep., 0033 Oslo, Norway. astrid.lovseth@vetinst.no
Astrid
A
Løvseth
Semir
S
Loncarevic
Knut G
KG
Berdal
D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D004768:Enterotoxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D018089:Superantigens / Q000235:genetics*
D001427:Bacterial Toxins; D017931:DNA Primers; D004768:Enterotoxins; D005098:Exotoxins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal