[Study on the extraction process for free isofraxidin and hydrolytic condition of combined isofraxidin in Ciwujia with orthogonal design].
OBJECTIVE
To optimize the extraction and hydrolytic condition of isofraxidin in Ciwujia.


METHODS
The study was carried out through orthogoral experimental design. The content of isofraxidin was determined as marker to evaluate the effect on the extraction and hydrolytic condition of different process.


RESULTS
The optimum extraction condition was 12 times methanol and 3 times for sixty minutes every time. And the optimum hydrolytic condition was that hydrolysis for two hours, ten times amount of 15% sulfuric acid at 100 degrees C.
Shenyang Pharmaceutical University, Shenyang 110016.
Xinrong
X
Yuan
Kaishun
K
Bi
Qiang
Q
Li
D002855:Chromatography, Thin Layer; D003374:Coumarins / Q000302:isolation & purification*; D028001:Eleutherococcus / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D003374:Coumarins; C008182:isofraxidin