Crystallization and preliminary X-ray analysis of pectin lyase A from Aspergillus niger.
The major secreted pectin lyase (E.C. 4.2.2.10) from Aspergillus niger, strain 4M-147, has been purified and crystallized by the hanging-drop method using polyethylene glycol as precipitant. The crystals belong to the space group P2(1)2(1)2(1) with cell dimensions a = 45.2, b = 83.2, c = 93.1 A (1 A = 0.1 nm) and a single molecule in the asymmetric unit. The crystals diffract to at least 2.0 A resolution and are suitable for structure determination.
Department of Protein Engineering, Institute of Food Research, Reading, England.
J
J
Jenkins
M
M
Scott
O
O
Mayans
R
R
Pickersgill
G
G
Harris
I
I
Connerton
T
T
Gravesen