Cloning of an agr homologue of Staphylococcus saprophyticus.
An agr homologue of Staphylococcus saprophyticus was identified, cloned and sequenced. The gene locus shows homologies to other staphylococcal agr systems, especially to those of S. epidermidis and S. lugdunensis. A putative RNAIII was identified and found to be differentially expressed during the growth phases. In contrast to the RNAIII molecules of S. epidermidis and S. aureus it does not contain an open reading frame that codes for a protein with homologies to the delta-toxin. Using PCR, the agr was found to be present in clinical isolates of S. saprophyticus.
Sakinc
Türkan
T
Institut für Hygiene und Mikrobiologie, Abteilung für Medizinische Mikrobiologie, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
Kulczak
Pawel
P
Henne
Karsten
K
Gatermann
Sören G
SG
D000222:Adaptation, Physiological; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D005786:Gene Expression Regulation; D023061:Gene Order; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification; D015534:Trans-Activators / Q000235:genetics*; D014552:Urinary Tract Infections / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D016372:RNA, Antisense; D012329:RNA, Bacterial; C096035:RNAIII, Staphylococcus aureus; D015534:Trans-Activators