The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1.
The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space. In this study, we used the yeast two-hybrid system to search for proteins that interact with the cytoplasmic carboxy terminal tail region of GlyT2. Screening of a rat brain cDNA library identified the PDZ domain protein syntenin-1 as an intracellular binding partner of GlyT2. In pull-down experiments, the interaction between GlyT2 and syntenin-1 was found to involve the C-terminal amino acid residues of GlyT2 and the PDZ2 domain of syntenin-1. Syntenin-1 is widely expressed in brain and co-localizes with GlyT2 in brainstem sections. Furthermore, syntenin-1 binds syntaxin 1A, which is known to regulate the plasma membrane insertion of GlyT2. Thus, syntenin-1 may be an in vivo binding partner of GlyT2 that regulates its trafficking and/or presynaptic localization in glycinergic neurons.
Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528 Frankfurt am Main, Germany.
Koji
K
Ohno
Michael
M
Koroll
Oussama
O
El Far
Petra
P
Scholze
Jesus
J
Gomeza
Heinrich
H
Betz
D000595:Amino Acid Sequence / Q000235:genetics; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D050827:Syntaxin 1; D053676:Syntenins; D020798:Two-Hybrid System Techniques
D026921:Amino Acid Transport Systems, Neutral; D000954:Antigens, Surface; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493271:SLC6A5 protein, human; C494212:STX1A protein, human; C492863:Sdcbp protein, rat; C493269:Slc6a5 protein, rat; C494213:Stx1a protein, rat; D050827:Syntaxin 1; D053676:Syntenins; D005998:Glycine