Transforming growth factor-beta3-loaded microtextured membranes for skin regeneration in dermal wounds.
Adverse effects of wound healing, such as excessive scar tissue formation, wound contraction, or nonhealing wounds represent a major clinical issue in today's healthcare. Transforming growth factor (TGF)-beta3 has specifically been implicated in wound healing. Our hypothesis was that local administration of TGF-beta3 to excisional dermal wounds would diminish wound contraction and scar formation. Microtextured wound covers, containing different concentrations of TGF-beta3, were placed onto full-thickness excisional skin wounds in guinea pigs. Tattooed reference marks were used to quantify wound contraction. Sixty-four male guinea pigs in four study groups (5 ng TGF-beta3, 50 ng TGF-beta3, no growth factor, sham wound) were followed for up to 6 weeks. We analyzed 19 different parameters of wound healing. Results showed that, in some instances, the 50-ng TGF-beta3 group gave less contraction, whereas the 5-ng TGF-beta3 group gave more contraction. These differences confirm that TGF-beta3 has an optimum working concentration, and suggest this concentration to be closer to 50 ng than to 5 ng TGF-beta3. However, only very few significant differences occurred, and thus we conclude that the clinical relevance of our findings is negligible. Earlier studies, reporting clinically improved wound healing by TGF-beta3, could therefore not be confirmed by this study.
Department of Biomaterials, College of Dental Science, University Medical Centre, PO Box 9101, 6500HB, Nijmegen, the Netherlands.
D P P
DP
Vooijs
X F
XF
Walboomers
J A T C
JA
Parker
J W
JW
Von den Hoff
J A
JA
Jansen
D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D012038:Regeneration / Q000502:physiology; D012828:Silicones / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000473:pathology*; D013499:Surface Properties; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism*; D053782:Transforming Growth Factor beta3; D014945:Wound Healing
D000199:Actins; D001672:Biocompatible Materials; D012828:Silicones; D016212:Transforming Growth Factor beta; D053782:Transforming Growth Factor beta3