Electrochemical vapor generation of selenium species after online photolysis and reduction by UV-irradiation under nano TiO2 photocatalysis and its application to selenium speciation by HPLC coupled with atomic fluorescence spectrometry.
An online UV photolysis and UV/TiO2 photocatalysis reduction device (UV-UV/TiO2 PCRD) and an electrochemical vapor generation (ECVG) cell have been used for the first time as an interface between high-performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) for selenium speciation. The newly designed ECVG cell of approximately 115 microL dead volume consists of a carbon fiber cathode and a platinum loop anode; the atomic hydrogen generated on the cathode was used to reduce selenium to vapor species for AFS determination. The noise was greatly reduced compared with that obtained by use of the UV-UV/TiO2 PCRD-KBH4-acid interface. The detection limits obtained for seleno-DL: -cystine (SeCys), selenite (Se(IV)), seleno-DL: -methionine (SeMet), and selenate (Se(VI)) were 2.1, 2.9, 4.3, and 3.5 ng mL(-1), respectively. The proposed method was successfully applied to the speciation of selenium in water-soluble extracts of garlic shoots cultured with different selenium species. The results obtained suggested that UV-UV/TiO2 PCRD-ECVG should be an effective interface between HPLC and AFS for the speciation of elements amenable to vapor generation, and is superior to methods involving KBH4.
Department of Chemistry and the MOE Key Laboratory of Analytical Sciences, Xiamen University, Xiamen 361005, China.
Jing
J
Liang
Qiuquan
Q
Wang
Benli
B
Huang
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005740:Gases; D036103:Nanotechnology; D010084:Oxidation-Reduction; D010777:Photochemistry; D012643:Selenium / Q000737:chemistry* / Q000145:classification; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
D005740:Gases; C009495:titanium dioxide; D014025:Titanium; D012643:Selenium