Upregulation of retinoic acid-inducible gene-I in T24 urinary bladder carcinoma cells stimulated with interferon-gamma.
Urinary bladder epithelial cells play an important role in the host defense against urinary tract infections. Interferon-gamma (IFN-gamma) is a potent cytokine that regulates immune responses by inducing multiple genes in many types of cells including urinary bladder epithelial cells. Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH-box family, which is involved in various reactions related to RNA metabolism, and is induced in leukemic cells by retinoic acid or in endothelial cells by lipopolysaccharide. We have studied the expression of RIG-I in T24 cells, a cell line derived from human urinary bladder epithelial carcinoma cells. IFN-gamma stimulated T24 cells to express RIG-I mRNA and protein in concentration- and time-dependent manners. Immunohistochemical analysis revealed the expression of RIG-I in the urinary bladder epithelium from a patient with chronic urinary tract infection and in a bladder epithelial carcinoma. We conclude that RIG-I may play some role in inflammatory reactions in the urinary tract epithelium.
Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Zaifu-cho, Japan. timaizum@cc.hirosaki-u.ac.jp
Tadaatsu
T
Imaizumi
Norito
N
Yagihashi
Masaharu
M
Hatakeyama
Koji
K
Yamashita
Akira
A
Ishikawa
Kageaki
K
Taima
Hidemi
H
Yoshida
Soroku
S
Yagihashi
Kei
K
Satoh
D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007700:Kinetics; D020365:RNA Helicases / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
D000970:Antineoplastic Agents; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D015847:Interleukin-4; D014212:Tretinoin; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases