Immunoassays for collagens in chondrocyte and cartilage explant cultures.
Quantitative immunoassays have been developed to measure the content, degradation, and synthesis of types II and IX collagens in hyaline cartilages. Some of these assays and their applications are described in this chapter. These and other assays are commercially available. The applications of these assays are discussed with examples from recent publications.
Department of Health Sciences, St. Lawrence College, Kingston, Ontario, Canada.
R Clark
RC
Billinghurst
Fackson
F
Mwale
Anthony
A
Hollander
Mirela
M
Ionescu
A Robin
AR
Poole
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002356:Cartilage / Q000737:chemistry* / Q000276:immunology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry* / Q000276:immunology; D024043:Collagen Type II / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D024121:Collagen Type X / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data
D000906:Antibodies; D024043:Collagen Type II; D024121:Collagen Type X