Woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids.
Two cauliform bacteria (CM243T and CM251) isolated by J. Poindexter from the Atlantic Ocean were characterized by 16S rRNA gene sequencing, TaqI restriction fragment length polymorphism and single-strand conformation polymorphism analyses of the internally transcribed 16S-23S rDNA spacer (ITS1) region, analysis of fatty acids from cellular lipids, mass spectrometry of polar lipids and physiological properties. The two strains showed very low diversity of polar lipids with diacyl-sulfoquinovosyl glycerols as the predominant lipids. The two bacterial strains were observed to have nearly identical 16S rRNA gene sequences and could not be differentiated by their ITS1 regions. The isolates differed from species of the genus Maricaulis by their 16S rRNA gene sequences, polar lipids and fatty acid patterns. On the basis of the genotypic analyses and estimations of phylogenetic similarities, physiological and chemotaxonomic characteristics, it is proposed that the isolates represent a new genus and species, for which the name Woodsholea maritima gen. nov., sp. nov. (type strain CM243T=VKM B-1512T=LMG 21817T) is proposed.
GBF - German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Wolf-Rainer
WR
Abraham
GBF - German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Carsten
C
Strömpl
BCCM/LMG Bacteria Collection, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Marc
M
Vancanneyt
Area de Microbiologia, Campus UIB - Edifici Guillem Colom, Universitat de les Illes Balears, Crta. Valldemossa km 7·5, 07122 Palma de Mallorca, Spain.
Antonio
A
Bennasar
BCCM/LMG Bacteria Collection, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Jean
J
Swings
GBF - German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Heinrich
H
Lünsdorf
Dept of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada.
John
J
Smit
The Macaulay Research Institute, Craigiebuckler, Aberdeen AB15 8QH, UK.
Edward R B
ERB
Moore
D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D042063:Caulobacteraceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification; D008853:Microscopy; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S