GMPOZ, a BTB/POZ domain nuclear protein, is a regulator of hormone responsive gene expression in barley aleurone.
GAMYB is a GA-responsive activator of hydrolase gene expression in the aleurone layer of germinated cereal grains. We have isolated a putative GAMYB-binding protein, GMPOZ, which contains a BTB/POZ domain found in certain animal transcriptional regulators. Although BTB/POZ domain proteins are numerous in plants, very few are yet characterized. We found that GMPOZ is nuclear localized and that GMPOZ mRNA is expressed highly in anthers as well as aleurone. Transient silencing of the GMPOZ gene suggests that GMPOZ is involved in hormone responsive gene expression in aleurone.
Woodger
Fiona J
FJ
CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia. woodger@rsbs.anu.edu.au
Jacobsen
John V
JV
Gubler
Frank
F
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D020868:Gene Silencing; D005809:Genes, Regulator / Q000235:genetics; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D018076:DNA, Complementary; D009687:Nuclear Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger