Comparison of sequence analysis and a novel discriminatory real-time PCR assay for detection and quantification of Lamivudine-resistant hepatitis B virus strains.
We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.
Wightman
Fiona
F
Department of Medicine, Monash University, Melbourne, Australia.
Walters
Tomos
T
Ayres
Anna
A
Bowden
Scott
S
Bartholomeusz
Angeline
A
Lau
Daryl
D
Locarnini
Stephen
S
Lewin
Sharon R
SR
D001483:Base Sequence; D003199:Computer Systems; D017931:DNA Primers; D014644:Genetic Variation; D006509:Hepatitis B / Q000175:diagnosis* / Q000188:drug therapy; D006515:Hepatitis B virus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology*; D008137:Longitudinal Studies; D016133:Polymerase Chain Reaction / Q000379:methods; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D019562:Viral Load
D017931:DNA Primers; D018894:Reverse Transcriptase Inhibitors; D019259:Lamivudine