Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India.
Strain Sd/3T (=MTCC 4374T=DSM 15820T), an arsenic-resistant bacterium, was isolated from a sand sample obtained from an arsenic-contaminated aquifer in Chakdah district in West Bengal, India (23 degrees 3' N 88 degrees 35' E). The bacterium was Gram-positive, rod-shaped, non-motile, endospore-forming and yellowish-orange pigmented. It possessed all the characteristics that conform to the genus Bacillus, such as it had A4beta murein type (L-orn-D-Asp) peptidoglycan variant, MK-7 as the major menaquinone and iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. Based on its chemotaxonomic and phylogenetic characteristics, strain Sd/3T was identified as a species of the genus Bacillus. It exhibited maximum similarity (95%) at the 16S rRNA gene level with Bacillus cohnii; however, DNA-DNA similarity with B. cohnii was 60.7%. Strain Sd/3T also exhibited a number of phenotypic differences from B. cohnii (DSM 6307T). These data suggest that Sd/3T represents a novel species of the genus Bacillus. The name Bacillus indicus sp. nov. is proposed.
Suresh
K
K
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
Prabagaran
S R
SR
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
Sengupta
S
S
Geological survey of India, GSI Complex, DK-6, Sector-II, Bidhannagar, Kolkata - 700 091, India.
Shivaji
S
S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001151:Arsenic / Q000494:pharmacology*; D001407:Bacillus / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005840:Gentian Violet; D007194:India; D008969:Molecular Sequence Data; D009068:Movement; D010457:Peptidoglycan / Q000737:chemistry; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010457:Peptidoglycan; D010619:Phenazines; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D005840:Gentian Violet; D001151:Arsenic