Molecular characterization of the nucleocapsid protein gene of Newcastle disease virus strains in Japan and development of a restriction enzyme-based rapid identifying method.
Nucleocapsid protein (NP) gene of Newcastle disease virus (NDV) strains mainly isolated in Japan from 1930 to 2001 was genetically characterized. By deduced amino acid sequence comparison, the N-terminal region (from 1 to 401 residues) of the NP protein was found to be highly conserved, while the C-terminal region was highly variable among the NDV isolates. A phylogenetic tree construct based on the nucleotide sequence of the complete NP gene revealed that the old (prior to 1970s) and the new (after 1980s) isolates could be classified into two major different groups, i.e., a group comprising virulent strains, and another group composed of avirulent strains. By restriction enzyme analysis using Pst I, none of the virulent strains were cleaved, while avirulent strains were cleaved. The results may be useful for simple primary screening test for differentiating NDV isolates.
Pham
H M
HM
Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Chang
K S
KS
Mase
M
M
Ohashi
K
K
Onuma
M
M
D000595:Amino Acid Sequence; D000818:Animals; D004262:DNA Restriction Enzymes; D014644:Genetic Variation; D007564:Japan; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics*; D014774:Virulence
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C409209:nucleoprotein, Newcastle disease virus; D004262:DNA Restriction Enzymes