The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae.
Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H(+)-ATPase and the plasma membrane H(+)-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H(+)-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.
Department of Medical Genetics and Child Development Medicine, University of Pécs, Szigeti ut 12, 7623 Pécs, Hungary. richard.kellermayer@aok.pte.hu
Richard
R
Kellermayer
Réka
R
Szigeti
Miklós
M
Kellermayer
Attila
A
Miseta
D002118:Calcium / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005838:Genotype; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D006180:Proton-Translocating ATPases; D005947:Glucose; D002118:Calcium