Identification of (+)-phyllocladene, (--)-sandaracopimaradiene, and (+)-kaurene as new fungal metabolites from fusicoccin-producing Phomopsis amygdali F6.
A chemical analysis of the diterpene hydrocarbons produced by fusicoccin-producing fungus Phomopsis amygdali F6 identified five phyllocladene-related tri- and tetracyclic diterpene hydrocarbons. The presence of (+)-phyllocladene, (--)-sandaracopimaradiene, (+)-isopimara-8,15-diene, and (+)-pimara-8(14),15-diene in the fungus was demonstrated by GC-MS, 1H-NMR, and [alpha]D measurements. (+)-Kaurene was also identified by GC-MS and chiral capillary GC. The possible biosynthetic relationship of these metabolites is discussed.
Kenmoku
Hiromichi
H
Course of the Science of Bioresources, The United Graduate School of Agricultural Sciences, Iwate University (Yamagata University), Wakaba-cho, Tsuruoka 997-8555, Japan.
Tanaka
Mio
M
Ogiyama
Koichi
K
Kato
Nobuo
N
Sassa
Takeshi
T
D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation
D004224:Diterpenes; D006027:Glycosides; C007808:fusicoccin