[Expression of proteinase cathepsin L1 gene of Schistosoma japonicum in Escherichia coli].
OBJECTIVE
To express the proteinase cathepsin L1 gene of Schistosoma japonicum (SjCL1) in Escherichia coli JM109 cells.


METHODS
The SjCL1 gene was amplified from the recombinant plasmid pcDNA3-SjCL1 by PCR. The gene was cloned into a prokaryotic expression vector pGEX4T-1 to construct a recombinant plasmid pGEX-SjCL1. The E. coli JM109 cells were transformed with the recombinant plasmid pGEX-SjCL1 and the transformants were induced by IPTG to express the recombinant protein, the target protein was then identified by SDS-PAGE and Western blotting.


RESULTS
A 1 kb length PCR product was obtained and a recombinant plasmid pGEX-SjCL1 was constructed. The expression product was detected by SDS-PAGE and Western blotting and an expression band about 62000 was found.


CONCLUSIONS
The SjCL1 gene is effectively expressed in the E. coli JM109 cells.
Department of Parasitology, Zhong Shan Medical College, Sun Yat-Sen University, Guangzhou 510099, China.
Bing
B
Yi
Zhuo-ya
ZY
Li
Ai
A
He
Xiao-ying
XY
Zheng
Bin
B
Zheng
Hao-jie
HJ
Zhou
Yi
Y
Wang
Rui-lin
RL
Zhang
Nan
N
Yu
Xi-mei
XM
Zhan
D000818:Animals; D002403:Cathepsins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D002403:Cathepsins