The Cl-/HCO3- exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance.
Pendrin (Pds; Slc26A4) is a new anion exchanger that is believed to mediate apical Cl(-)/HCO(3)(-) exchange in type B and non-A-non-B intercalated cells of the connecting tubule and cortical collecting duct. Recently, it has been proposed that this transporter may be involved in NaCl balance and blood pressure regulation in addition to its participation in the regulation of acid-base status. The purpose of our study was to determine the regulation of Pds protein abundance during chronic changes in chloride balance. Rats were subjected to either NaCl, NH(4)Cl, NaHCO(3), KCl, or KHCO(3) loading for 6 days or to a low-NaCl diet or chronic furosemide administration. Pds protein abundance was estimated by semiquantitative immunoblotting in renal membrane fractions isolated from the cortex of treated and control rats. We observed a consistent inverse relationship between Pds expression and diet-induced changes in chloride excretion independent of the administered cation. Conversely, NaCl depletion induced by furosemide was associated with increased Pds expression. We conclude that Pds expression is specifically regulated in response to changes in chloride balance.
Institut National de la Santé et de la Recherche Médicale Unité 356, Institut Fédératif de Recherche 58, Université René Descartes, Paris, France.
Fabienne
F
Quentin
Régine
R
Chambrey
Marie Marcelle
MM
Trinh-Trang-Tan
Marinos
M
Fysekidis
Michèle
M
Cambillau
Michel
M
Paillard
Peter S
PS
Aronson
Dominique
D
Eladari
D000643:Ammonium Chloride / Q000008:administration & dosage; D000818:Animals; D001639:Bicarbonates / Q000008:administration & dosage; D002462:Cell Membrane / Q000737:chemistry; D027963:Chloride-Bicarbonate Antiporters / Q000032:analysis*; D002712:Chlorides / Q000008:administration & dosage / Q000378:metabolism*; D004032:Diet; D005665:Furosemide / Q000008:administration & dosage; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D015151:Immunoblotting; D007668:Kidney / Q000737:chemistry*; D008297:Male; D011189:Potassium Chloride / Q000008:administration & dosage; D017680:Potassium Compounds / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D017693:Sodium Bicarbonate / Q000008:administration & dosage; D012965:Sodium Chloride / Q000008:administration & dosage; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage; D000076988:Sulfate Transporters
D001639:Bicarbonates; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D017680:Potassium Compounds; C494615:Slc26A4 protein, rat; D017673:Sodium Chloride, Dietary; D000076988:Sulfate Transporters; D000643:Ammonium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D005665:Furosemide; D017693:Sodium Bicarbonate; C026329:potassium bicarbonate