A partial sequence of lipoxygenase gene from genomic DNA of aromatic rice (Oryza sativa L.).
Aromatic (Bas-370, PB-1) and non-aromatic (Pusa-677, Pusa-834) rice were selected for the characterization and for distribution of lipoxygenase (Lox) genes. Polymorphism was observed when genomic DNA of rice varieties was hybridized with a heterologous lipoxygenase probe. A distinct polymorphic fragment (approximately 1.2 kb) was found in Bas-370. Sub-genomic library of Bas-370 was constructed and screened with LoxA probe. The smallest putative clone (pBas-14) of approximately 1.2 kb was sequenced. Complete nucleotide and deduced amino acid sequence showed the clone was 1134 bp long and comprised of 378 amino acid residues. PCR amplification of genomic DNA from four rice varieties with a soybean Lox primer also showed a polymorphic fragment of size approximately 600 bp (amplicon) in aromatic varieties that was sequenced directly. Nucleotide sequence alignment between pBas-14 and amplicon concluded that the amplicon was a part of the insert pBas-14.
Basak
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S
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India.
Sachdev
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A
Johari
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RP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant; D015698:Genomic Library; D008084:Lipoxygenase / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D015342:DNA Probes; D018744:DNA, Plant; D010940:Plant Proteins; D008084:Lipoxygenase