Myristic acid increases delta6-desaturase activity in cultured rat hepatocytes.
In order to study the effects of saturated fatty acids on delta6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic (C16:0) acids. After optimization, the standard in vitro conditions for the measurement of delta6-desaturase activity were as follows: 60 micromol x L(-1) alpha-linolenic acid (C18:3n-3), reaction time of 20 min and protein content of 0.4 mg. Data showed that cell treatment with 0.5 mmol x L(-1) myristic acid during 43 h specifically increased delta6-desaturase activity. This improvement, reproducible for three substrates of delta6-desaturase, i.e. oleic acid (C18:1n-9), linoleic acid (C18:2n-6) and alpha-linoleic acid (C18:3n-3) was dose-dependent in the range 0.1-0.5 mmol x L(-1) myristic acid concentration.
Laboratoire de Biochimie, INRA-ENSA, 65 rue de Saint-Brieuc, CS84215, 35042 Rennes, France.
Sophie
S
Jan
Hervé
H
Guillou
Sabine
S
D'Andrea
Stéphanie
S
Daval
Monique
M
Bouriel
Vincent
V
Rioux
Philippe
P
Legrand
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D044943:Fatty Acid Desaturases / Q000187:drug effects / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology*; D007850:Lauric Acids / Q000494:pharmacology; D050602:Linoleoyl-CoA Desaturase; D008297:Male; D019814:Myristic Acid / Q000494:pharmacology*; D019308:Palmitic Acid / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D007850:Lauric Acids; D019814:Myristic Acid; C030358:lauric acid; D019308:Palmitic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase