Alanine-threonine polymorphism of Helicobacter pylori RpoB is correlated with differential induction of interleukin-8 in MKN45 cells.
Geographical differences in the genetic diversity of Helicobacter pylori isolates were examined by analyzing rpoB sequences. An extremely high level of allelic diversity among H. pylori strains was found. The rpoB sequences of Asian and non-Asian (North and South American, European, and South African) strains were found to differ. An amino acid polymorphism (alanine and threonine RpoB types) was found at the 497th residue by deduced amino acid analysis. RpoB with a threonine residue (RpoB(Thr)) was uniquely present in East Asian countries, and two-thirds of the H. pylori isolate population in this region was RpoB(Thr); however, this type was rare or absent in Western countries, where RpoB(Ala) predominated. RpoB(Thr) strains induced a much larger amount of interleukin-8, a chemokine that plays an important role in chronic inflammation, than RpoB(Ala) strains in cultured MKN45 cells.
Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-799, Korea.
Keun-Hwa
KH
Lee
Myung-Je
MJ
Cho
Yoshio
Y
Yamaoka
David Y
DY
Graham
Yeo-Jun
YJ
Yun
So-Yon
SY
Woo
Chang-Young
CY
Lim
Kwan-Soo
KS
Ko
Bum-Joon
BJ
Kim
Hyun-Chae
HC
Jung
Woo-Kon
WK
Lee
Kwang-Ho
KH
Rhee
Yoon-Hoh
YH
Kook
D000409:Alanine; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018920:Coculture Techniques; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000276:immunology; D005843:Geography; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016209:Interleukin-8 / Q000096:biosynthesis*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013912:Threonine
D017931:DNA Primers; D004269:DNA, Bacterial; D016209:Interleukin-8; D013912:Threonine; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D000409:Alanine