Differentiation of Pasteurella multocida isolates from cases of atrophic rhinitis in pigs from Zimbabwe by RAPD and ribotyping.
Atrophic rhinitis in pigs is rarely reported in Southern Africa. To determine the relationship between Pasteurella multocida clones from clinical cases of atrophic rhinitis, twenty-one strains were characterised by selected phenotypic and genotypic methods. Biochemical analysis classified 18 strains as P. multocida subspecies multocida, whilst the remainder were grouped into separate unassigned biotypes. Capsular groups A (16/21) and D (l/21) were found among the isolates by PCR. Four ribotype patterns were obtained following HpaII ribotyping, whilst random amplification of polymorphic DNA (RAPD) revealed three main clusters. However, subclusters were also noted for each RAPD cluster. Our results indicate that RAPD offers a better discrimination of strains than ribotyping and that none of the phenotypic characters were directly related to the genotypic clusters.
Faculty of Veterinary Science, University of Zimbabwe, PO Box MP167, Mount Pleasant, Harare. francis.dziva@bbsrc.ac.uk
F
F
Dziva
H
H
Christensen
L A M G
LA
van Leengoed
K
K
Mohan
J E
JE
Olsen
D000818:Animals; D016667:Bacterial Capsules / Q000145:classification / Q000235:genetics; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D012222:Rhinitis, Atrophic / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D021521:Ribotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D015030:Zimbabwe / Q000453:epidemiology
D004269:DNA, Bacterial