[Degradation of the herbicide atrazine by the soil mycelial fungus INBI 2-26(-)--a producer of cellobiose dehydrogenase].
Nonsporulating mycelial fungi producing cellobiose dehydrogenase (CDH) and isolated from soils of South Vietnam with high residual content of dioxins are capable of growing on a solid medium in the presence of high atrazine concentrations (to 500 mg/l). At 20 and 50 mg/l atrazine, the area of fungal colonies was 1.5-1.2-fold larger, respectively, compared with control colonies of the same age, whereas development of the colonies at 500 mg/l atrazine was delayed by 5 days, compared with controls grown in the absence of atrazine. Surface cultivation of the fungus on a minimal medium with glucose as a sole source of carbon and energy decreased the initial concentration of atrazine (20 mg/l) 50 times in 40 days; in addition, no pronounced sorption of atrazine by mycelium was detected. This was paralleled by accumulation in the culture medium of extracellular CDH; atrazine increased the synthesis of this enzyme two- to threefold. Accumulation of beta-glucosidase (a mycelium-associated enzyme) and cellulases preceded the formation of CDH.
Khromonygina
V V
VV
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Saltykova
A I
AI
Vasil'chenko
L G
LG
Kozlov
Iu P
IuP
Rabinovich
M L
ML
D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002237:Carbohydrate Dehydrogenases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D044602:Cellulases / Q000032:analysis / Q000096:biosynthesis; D003470:Culture Media; D004147:Dioxins / Q000032:analysis; D005658:Fungi / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism*; D025282:Mycelium; D012988:Soil Microbiology; D013997:Time Factors; D014744:Vietnam; D001617:beta-Glucosidase / Q000032:analysis / Q000096:biosynthesis
D003470:Culture Media; D004147:Dioxins; D006540:Herbicides; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D044602:Cellulases; D001617:beta-Glucosidase; D001280:Atrazine