Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification.
Metabolite, protein, and transcript analysis at the cellular level gives unparalleled insight into the complex roles tissues play in the plant system. However, while capillary electrophoresis and PCR amplification strategies make the profiling of metabolites and transcripts in specific cell types possible, the profiling of proteins in small samples represents a bottleneck. Here for the first time protein profiling has been achieved in a specific plant cell type: The application of specific cell sampling and shotgun peptide sequencing (nano LC/MS/MS) resulted in the identification of 63 unique proteins from pooled Arabidopsis trichome cells. A complete S-adenosylmethionine pathway cluster, two S-adenosylmethionine synthase isoforms, a glutathione S-conjugate translocator and other proteins involved in sulfur metabolism and detoxification are shown to be present in these cells, in agreement with previous work done at the level of trichome transcript analysis. The technology described here brings the simultaneous identification and localization of physiologically relevant cellular proteins within reach.
Wienkoop
Stefanie
S
Max Planck Institute of Molecular Plant Physiology, Metabolic Networks, 14424 Potsdam, Germany.
Zoeller
Daniela
D
Ebert
Berit
B
Simon-Rosin
Ulrike
U
Fisahn
Joachim
J
Glinski
Mirko
M
Weckwerth
Wolfram
W
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*
D029681:Arabidopsis Proteins; D013455:Sulfur