High resolution MRI relaxation measurements of water in the articular cartilage of the meniscectomized rat knee at 4.7 T.
Measurements by magnetic resonance imaging (MRI) of the spin-spin (T2), spin-lattice (T1) and spin-density (M0) parameters of water protons, optimized by using the Cramér-Rao Lower Bound (CRLB) theory, were made to quantify the effect of surgically induced osteoarthritis on rat knee cartilage at 4.7 T. Partial meniscectomy was performed on the right medial condyle of four Sprague Dawley rats, leaving the left medial condyle as a control. The animals were euthanized 3 weeks after the operation; the entire limbs were removed and T2 and T1 relaxation measurements and M0 measurements of the protons of water were obtained using conventional Carr-Purcell-Meiboom-Gill (CPMG) and saturation recovery methods. M0 was normalized with respect to a water phantom, to obtain the relative spin-density M0%. Weight-bearing cartilage areas on the meniscectomized medial condyles exhibited a significant increase of T2 relaxation time (p < 0.001) and of M0% (p < 0.01) with respect to the control; T1 relaxation times did not show any statistically significant changes. CRLB-based sampling optimization offered an insight to improved measurement precision and a reduction of scanning time against conventional sampling methods methods. Quantitative MRI assessment of the meniscectomized rat knee shows that cartilage exhibits changes in T2 and M0 values 3 weeks after operation.
Herchel Smith Laboratory for Medicinal Chemistry, University of Cambridge School for Clinical Medicine, CB2-2PZ UK.
Yiannis
Y
Spandonis
Frank P
FP
Heese
Laurance D
LD
Hall
D000818:Animals; D001834:Body Water; D002358:Cartilage, Articular / Q000473:pathology* / Q000601:surgery; D007719:Knee Joint / Q000473:pathology* / Q000601:surgery; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010003:Osteoarthritis / Q000473:pathology* / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric