Hyperpolarization by glucose of feeding-related neurons in snail.
In the pond snail Lymnaea stagnalis, D-glucose action was investigated on electrical activity of identified central neurons. In the CNS preparations isolated from specimens that starved for 24-96 h, D-glucose added to a standard or HiDi saline at 500-700 microg/ml effectively hyperpolarized ca. 90% of feeding related neurons B1, SO and CGC. However, not all feeding-related neurons examined were responsive to glucose. Experiments on cells of the serotonergic Pedal A cluster have shown that hyperpolarizing action of D-glucose is retained following complete isolation of "hunger" neurons. Threshold concentration producing 1-3 mV hyperpolarization was ca. 50 microg/ml. The results suggest a direct glucose involvement in the mechanisms that control feeding behavior in Lymnaea.
Alania
Magda
M
Laboratory of Comparative Physiology, Institute for Developmental Biology, RAS, 26 Vavilov str., Moscow, 119909 Russia.
Dyakonova
Varya
V
Sakharov
D A
DA
D000818:Animals; D001522:Behavior, Animal; D002490:Central Nervous System / Q000378:metabolism; D005247:Feeding Behavior; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D009424:Nervous System Physiological Phenomena; D009474:Neurons / Q000378:metabolism*; D012908:Snails; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D012965:Sodium Chloride; D005947:Glucose