Expression of neurokinin a receptor mRNA in human nasal mucosa.
In airway tissues, it has been suggested that tachykinins act as the transmitter for afferent sensory nerves which respond to various irritants and may be involved in airway allergic reactions. Three classes of tachykinin receptor have been recognized, denoted NK1, NK2 and NK3, which exhibit preferential affinity for substance P, neurokinin A and neurokinin B, respectively. We used molecular probes to study the gene expression and distribution of NK2 receptor in human nasal mucosa. Total RNA was isolated from human nasal mucosa and NK2 receptor mRNA was detected in these tissues using reverse transcriptase polymerase chain reaction (RT-PCR). For an in situ hybridization study of human nasal mucosa, we utilized the PCR directly to incorporate a T7 RNA polymerase promoter sequence onto the NK2 receptor cDNA, and these PCR products were used as the DNA template for producing digoxigenin-labeled antisense and sense RNA probes. These studies revealed that NK2 receptor mRNA was expressed in blood vessels. The results suggest a primary role for neurokinin A in the form of vascular responses in the upper respiratory tract.
Shirasaki
Hideaki
H
Department of Otolaryngology, School of Medicine, Sapporo Medical University, Japan. puchibe@spa.att.ne.jp
Kanaizumi
Etsuko
E
Watanabe
Kazumasa
K
Konno
Nobuhiro
N
Sato
Jun
J
Asakura
Kohji
K
Kataura
Akikatsu
A
Himi
Tetsuo
T
D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism*; D015508:Nasal Obstruction / Q000209:etiology / Q000235:genetics; D015288:Neurokinin A / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D018041:Receptors, Neurokinin-2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014420:Turbinates / Q000378:metabolism
D012333:RNA, Messenger; D018041:Receptors, Neurokinin-2; D015288:Neurokinin A