Green tea flavan-3-ols and oligomeric proanthocyanidins inhibit the motility of infective larvae of Teladorsagia circumcincta and Trichostrongylus colubriformis in vitro.
The effects of a hot water infusion and an aqueous acetone extract of green tea (Camellia sinensis) on the motility of infective larvae of the sheep nematodes Teladorsagia circumcincta and Trichostrongylus colubriformis were investigated under in vitro conditions. The infusion and extract dose-dependently inactivated the infective larvae as assessed by the larval migration inhibition (LMI) assay. To determine the components responsible for the inhibitory activity, the hot water infusion and aqueous acetone extract of green tea were fractionated on Sephadex LH-20 and the green tea extract fractions (GTE-I-VIII) characterised by mass spectrometry. The larvae were exposed to increasing concentrations of these GTE fractions. Fractions containing epigallocatechin gallate (EGCG) and proanthocyanidin oligomers were most effective. GTE fractions were more effective against T. circumcincta than T. colubriformis larvae as assessed by the LMI assay.
Nutrition and Behaviour, AgResearch Ltd., Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand.
A L
AL
Molan
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S
Sivakumaran
P A
PA
Spencer
L P
LP
Meagher
D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000469:parasitology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D013058:Mass Spectrometry; D010028:Ostertagia / Q000187:drug effects* / Q000302:isolation & purification; D010029:Ostertagiasis / Q000469:parasitology / Q000662:veterinary; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013662:Tea; D014253:Trichostrongylosis / Q000469:parasitology / Q000662:veterinary; D014254:Trichostrongylus / Q000187:drug effects* / Q000302:isolation & purification
D000981:Antiprotozoal Agents; D005419:Flavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; D013662:Tea; C404987:flavan-3-ol