Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40.
In the 'post-genome' era, reverse genetics is one of the most informative and powerful means to investigate protein function. The chicken B lymphocyte line DT40 is widely used for reverse genetics because the cells have a number of advantages, including efficient gene targeting as well as a remarkably stable phenotype. Furthermore, the absence of functional p53 in DT40 cells enables identification of DNA damage using chromosome analysis by suppressing damage-induced apoptosis during interphase. This review summarizes the contribution of DT40 cells to reverse genetic studies of DNA damage response pathways in higher eukaryotic cells.
Yamazoe
Mitsuyoshi
M
CRESTO, The Japan Science and Technology Corporation, Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo-ku, Kyoto 606-8501, Japan.
Sonoda
Eiichiro
E
Hochegger
Helfrid
H
Takeda
Shunichi
S
D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D002645:Chickens; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D016158:Genes, p53; D005821:Genetic Techniques; D007399:Interphase; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic
D004247:DNA