Genomic structure and multiple alternative transcripts of the mouse TRAD/RAD51L3/RAD51D gene, a member of the recA/RAD51 gene family.
The RecA/RAD51 family plays a central role in DNA recombinational repair. The targeted disruption of mouse RAD51L3/TRAD is lethal during embryogenesis, suggesting that this protein is essential for development. Recently, we reported multiple alternative splice variants of human RAD51L3/TRAD transcripts. In this study, we have identified multiple mouse transcript variants. Complete sequence analysis of the genomic and cDNA clones has confirmed that the exon-intron structures obey the GT/AG splicing rule, and that the multiplicity of the transcripts is due to alternative splicing. In addition, we have determined the transcription initiation site by rapid amplification of cDNA 5'-end (5'-RACE). These results show that the mouse gene structure is very similar to that of humans.
Kawabata
Masahiro
M
Department of Neuroscience, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. mbat@md.okayama-u.ac.jp
Akiyama
Kousuke
K
Kawabata
Teruyuki
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D051379:Mice; D008969:Molecular Sequence Data; D011938:Rec A Recombinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D024363:Transcription Initiation Site
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C491807:Rad51d protein, mouse; D011938:Rec A Recombinases