Fibrin glue for the suture-less correction of penile chordee: a pilot study in a rabbit model.
OBJECTIVE
To evaluate the use of fibrin glue as a scaffold for patching defects in the tunica albuginea in a rabbit model for a future application in correcting chordee.


METHODS
Nine New Zealand white male rabbits were utilized. All had a 15 x 5-mm defect created in the ventral tunica albuginea. Fibrin glue (1 mL) was applied to cover the defect in tunica albuginea and the penile skin closed with a continuous 5/0 chromic catgut suture. Animals were killed in groups of three at 2, 6 and 12 weeks afterward. The evaluation included an artificial erection test with intracavernosal injection of prostaglandin E1 (5 microg), cavernosography and histopathological examination of sections of the penis stained with haematoxylin and eosin or Masson trichrome.


RESULTS
None of the rabbits died during the procedure or developed bleeding or haematoma afterward. All animals had straight erections on testing with prostaglandin (5 microg). There was no evidence of corporal narrowing or venous leakage on cavernosography. Histopathological evaluation showed evidence of the fibrin sealant layer, with angiogenesis and a cell infiltrate at 2 weeks. At 6 and 12 weeks there was completely normal regeneration of the tunica albuginea.


CONCLUSIONS
In this pilot study in a rabbit model the haemostatic effect of fibrin glue was confirmed on covering a defect in the tunica albuginea. Moreover, there was regeneration of normal tunica albuginea with no scarring at 6 weeks and maintained at 12 weeks. Further well-controlled studies are required before using fibrin glue for corporal body grafting to treat chordee.
Urology & Nephrology Centre, Mansoura University, Mansoura, Egypt.
Ashraf T
AT
Hafez
Ahmed
A
El-Assmy
Mohamed A
MA
El-Hamid
D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D006490:Hemostatics / Q000627:therapeutic use*; D007021:Hypospadias / Q000601:surgery*; D008297:Male; D010410:Penile Erection; D010413:Penis / Q000601:surgery*; D010865:Pilot Projects; D011817:Rabbits; D014014:Tissue Adhesives / Q000627:therapeutic use*; D014945:Wound Healing
D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D014014:Tissue Adhesives