[Study of Magnolia grandiflora extracts in guinea pigs cardiac muscle].
Several extracts from diverse Magnolia grandiflora varieties were pharmacological evaluated in the cardiac muscle. From March to July, flowers and leaves from Magnolia grandiflora, native from the National Institute of Cardiology "Ignacio Chávez", from north, west, and orient zones from Mexico City, and from Puebla, Colima and Chiapas states were collected. They were separately processed and the extracts were obtained by maceration with ethanol-water (1:3 v/v) at 4 degrees C during two weeks. Qualitative analysis was accomplished with thin-layer, column and high-performance liquid chromatographies (HPLC). Functional and molecular analysis was made by specific chemical reactivity and by protonic magnetic resonance (RMN 1H). Pharmacological evaluation was completed in isolated and perfused male guinea pigs hearts. Extracts, fractions, and compounds were administrated by serial bolus in a gradual dose-response curves study in which left intraventricular pressure and coronary perfusion pressure were recorded, evaluating by such the positive inotropic and vasodilator effects of Magnolia grandiflora extracts. Vulgarenol and 2-p-hydroxyphenyl-2-hydroxy-ethylamine were isolated and identified, and the obtained results suggest that its positive inotropic and vasodilator effects are owed to these substances, being complemented by magnograndiolide and tyramine.
Departamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chavez, Tlalpan, México, DF. leonardodvm65@hotmail.com
Leonardo
L
del Valle Mondragón
Fermín Alejandro
FA
Tenorio López
Juan Carlos
JC
Torres Narváez
Gabriela
G
Zarco Olvera
Gustavo
G
Pastelín Hernández
D000818:Animals; D016022:Case-Control Studies; D002850:Chromatography, Gel; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D031566:Magnolia; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D016277:Ventricular Function, Left / Q000187:drug effects / Q000502:physiology; D017725:Ventricular Pressure / Q000187:drug effects / Q000502:physiology
D010936:Plant Extracts