Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications.
Recent developments in single molecule force spectroscopy (SMFS) allow direct observation and measurements of forces that hold protein-DNA complexes together. Furthermore, the mechanics of double-stranded (ds) DNA molecules in the presence of small binding ligands can be detected. The results elucidate molecular binding mechanisms and open the way for ultra sensitive and powerful biosensor applications.
Experimental Biophysics, Faculty of Physics, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany. robert.ross@physik.uni-bielefeld.de
Robert
R
Ros
Rainer
R
Eckel
Frank
F
Bartels
Andy
A
Sischka
Birgit
B
Baumgarth
Sven David
SD
Wilking
Alfred
A
Pühler
Norbert
N
Sewald
Anke
A
Becker
Dario
D
Anselmetti
D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*; D004548:Elasticity; D008024:Ligands; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D009690:Nucleic Acid Conformation; D010812:Physical Stimulation / Q000295:instrumentation / Q000379:methods; D011485:Protein Binding; D011487:Protein Conformation; D013314:Stress, Mechanical
D004268:DNA-Binding Proteins; D008024:Ligands; D004247:DNA