Isolation and characterization of a new cell line from a renal carcinoma bone metastasis.
BACKGROUND
The use of cell lines isolated from metastases should enable the assessment of peculiar aspects of bone resorbing cytokine expression, as well as angiogenetic activity of renal carcinoma bone metastases.


METHODS
A cell line (CRBM-1990) was isolated from a renal cell carcinoma bone metastasis and compared with the ACHN and Caki-1 established lines. The expression of osteolytic cytokine and angiogenetic growth factors mRNAs, as well as the effect on migration and proliferation of a bovine bone cell line (BBE) were determined.


RESULTS
There were no significant differences between the three lines in IL-6, TGF-beta, VEGF-A, VEGF-B, VEGF-C and FGF-2 mRNAs expression. VEGF-D, PIGF, or RANK-L-specific mRNA were not expressed. CRBM-1990-, Caki-1- and ACHN-conditioned media significantly stimulated the migration and proliferation of BBE.


CONCLUSIONS
CRBM-1990 expressed IL-6-specific mRNA, but not RANK-L, expressed angiogenetic growth factors and induced migration and proliferation of bone endothelial cells at a non-significantly different level when compared with Caki-1 and ACHN.
Laboratory for Pathophysiology of Orthopedic Implants, Istituti Ortopedici Rizzoli, Bologna, Italy.
Sofia
S
Avnet
Elisabetta
E
Cenni
Donatella
D
Granchi
Francesca
F
Perut
Ilaria
I
Amato
Luca
L
Battistelli
Maria Luisa
ML
Brandi
Armando
A
Giunti
Nicola
N
Baldini
D000368:Aged; D042501:Angiogenic Proteins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism / Q000556:secondary*; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000556:secondary*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D002417:Cattle; D045744:Cell Line, Tumor; D002633:Chemotaxis; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins; D019859:Proto-Oncogene Proteins c-met; D053245:RANK Ligand; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D053246:Receptor Activator of Nuclear Factor-kappa B; D017978:Receptors, Growth Factor; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
D042501:Angiogenic Proteins; D002352:Carrier Proteins; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D017978:Receptors, Growth Factor; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human; D016212:Transforming Growth Factor beta; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met