[Transformation of phospholipids by cabbage phospholipase D in mixed micelles containing 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate].
We compared the activities of cabbage phospholipase D during hydrolysis and transesterification of phosphatidylcholine in mixed micelles of surface-active compounds with various physicochemical properties. Mixed micelles of phospholipids and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (ratio, 1:2) were among the best substrates. Hydrolysis and transphosphatidylation were studied in micelles containing 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Mixed micelles of phosphatidylcholine and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate may serve as a new substrate for measurements of phospholipase D activity and preparative isolation of phospholipids using this enzyme.
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141 Belarus.
V M
VM
Shnigir
M A
MA
Kisel'
D001937:Brassica / Q000201:enzymology*; D002793:Cholic Acids / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D004951:Esterification; D006868:Hydrolysis; D008823:Micelles; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010739:Phospholipase D / Q000032:analysis / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D002793:Cholic Acids; D003902:Detergents; D008823:Micelles; D010713:Phosphatidylcholines; D010743:Phospholipids; D010739:Phospholipase D; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate