Diffusion of calcitonin through the wall of the root canal.
The aim of this study was to evaluate the in vitro diffusion of synthetic salmon calcitonin (CT), used as an intracanal medication, to the external root surface, with or without the presence of intact root cementum. Fifty-four human central incisors were used in the experiment, and were divided into two groups of 21 (test groups) and two groups of 6 teeth (control groups). After root canal preparation, 10 mul of calcitonin was inserted within the root canal chamber. The root was sealed and made externally impermeable. Specimens were then placed in tubes with saline solution buffered with phosphates and stored at 37 degrees C. The diffusion of calcitonin was measured after 1, 4 and 7 days. To count calcitonin present at the external media (PBS), ELISA test (an antigen-antibody reaction) was used. Results showed that there was calcitonin diffusion through dentin in all of the test samples. The absence of cementum increased the diffusion of calcitonin (p=0.05). The highest counts of CT were obtained on day 7 for groups with or without cementum - showing a direct relation between time and diffusion of the medication.
Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, Canada.
Selma Cristina Cury
SC
Camargo
Giulio
G
Gavini
Carlos Eduardo
CE
Aun
Douglas
D
Waterfield
Jeffrey M
JM
Coil
D000818:Animals; D002116:Calcitonin / Q000737:chemistry*; D003738:Dental Cements; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure; D003806:Dentin Permeability; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D012388:Root Canal Irrigants / Q000737:chemistry*; D018915:Root Canal Preparation; D013997:Time Factors; D014092:Tooth Root / Q000737:chemistry*
D003738:Dental Cements; D012388:Root Canal Irrigants; C028815:salmon calcitonin; D002116:Calcitonin