A CDC6-like factor from the archaea Sulfolobus solfataricus promotes binding of the mini-chromosome maintenance complex to DNA.
The archaeal replication apparatus appears to be a simplified version of the eukaryotic one with fewer polypeptides and simpler protein complexes. Herein, we report evidence that a Cdc6-like factor from the hyperthermophilic crenarchaea Sulfolobus solfataricus stimulates binding of the homohexameric MCM-like complex to bubble- and fork-containing DNA oligonucleotides that mimic early replication intermediates. This function does not require the Cdc6 ATP and DNA binding activities. These findings may provide important clues to understanding how the DNA replication initiation process has evolved in the more complex eukaryotic organisms.
Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131-Napoli, Italy.
Mariarita
M
De Felice
Luca
L
Esposito
Biagio
B
Pucci
Mariarosaria
M
De Falco
Mosè
M
Rossi
Francesca M
FM
Pisani
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D001105:Archaea / Q000378:metabolism; D019843:Archaeal Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000502:physiology; D019847:Chromosomes, Archaeal; D004261:DNA Replication; D019641:DNA, Archaeal / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D047468:Immunoprecipitation; D026341:Minichromosome Maintenance 1 Protein / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D011485:Protein Binding; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000502:physiology; D016944:Sulfolobus / Q000378:metabolism*
D019843:Archaeal Proteins; C066503:CDC6 protein, S cerevisiae; D018797:Cell Cycle Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D026341:Minichromosome Maintenance 1 Protein; D009841:Oligonucleotides; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate