Determination of quinolones in water samples by solid-phase extraction and liquid chromatography with fluorimetric detection.
A method is reported for the determination, in water samples, of 10 quinolones which are used as veterinary drugs. Analytes are isolated from samples by solid-phase extraction (SPE) and analysed by reversed-phase high-performance liquid chromatography using fluorimetric detection. A solid-phase extraction procedure based on retention on HBL OASIS cartridges and elution with a mixture of acetonitrile-water in basic medium is suitable for pre-concentration of the analytes. Pre-concentration factors up to 250 can be obtained. The quinolones are separated with an octyl silica-based column and mobile phases consisting of aqueous oxalic acid solutions and acetonitrile mixtures. The attained detection limits of the whole process are in the ng l(-1) level when 250 ml of water sample is processed. Recovery rates, from natural water samples spiked at 2060 ng l(-1) level, range from 70 to 100% and common standard deviation are about 6-12%.
Prat
M D
MD
Departament de Química Analítica, Universitat de Barcelona, Diagonal 647 Martí i Franquès, 1-11 E-08028 Barcelona, Spain. prat@apolo.qui.ub.es
Benito
J
J
Compañó
R
R
Hernández-Arteseros
J A
JA
Granados
M
M
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015363:Quinolones / Q000032:analysis*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000900:Anti-Bacterial Agents; D015363:Quinolones; D014874:Water Pollutants, Chemical