Liver injury after ischemia and reperfusion: the role of oxygen free radicals.
OBJECTIVE
This article describes conditions of ischemia and reperfusion in dog's liver. The aim of the study was to observe hepatic injury from oxygen free radicals and to evaluate mannitol and ascorbic acid as scavengers.


METHODS
The criteria for evaluation were histopathological and biochemical. Twenty-one dogs were divided into three equal groups: group A with 90 minutes of ischemia and 10 minutes of reperfusion, and groups B and C with 120 minutes of ischemia and 10 minutes of reperfusion. In treatment group C, before the ischemia, mannitol and ascorbic acid were injected.


RESULTS
Differences were significant for malondialdehyde in A and B group postischemia (p < 0.001). In group C, a significant difference (p < 0.091) was not observed. The histopathological examination revealed fatty degeneration, alterations of mitochondrial structure, and membranic cysts. In group C, the alterations were considerably milder.


CONCLUSIONS
We can suggest the use of ascorbic acid and mannitol at the clinical level for scavenging and inhibition of oxygen free radicals in ischemic liver.
Medical Corp. Hellenic Army, Grigoriou E 25, Drama, Greece.
Stavros
S
Tselepidis
Lysimahos
L
Papazoglou
Agellos
A
Dessiris
Ioannis
I
Vlemas
Georgios
G
Papageorgiou
Athanasia
A
Stournara
Anastasios
A
Minas
D032761:Animal Experimentation; D000818:Animals; D004285:Dogs; D007511:Ischemia / Q000150:complications*; D008099:Liver / Q000293:injuries*; D017382:Reactive Oxygen Species / Q000009:adverse effects*; D015424:Reperfusion / Q000009:adverse effects*
D017382:Reactive Oxygen Species