Human cervical mucus can act in vitro as a selective barrier against spermatozoa carrying fragmented DNA and chromatin structural abnormalities.
OBJECTIVE
We have carried out experiments to determine if human cervical mucus can act as an in vitro selective barrier against spermatozoa morphologically normal that carry genetic structural abnormalities.


METHODS
Sperm chromatin abnormalities have been evaluated by Chromomycin A3 and "endogenous" nick translation.


RESULTS
The data obtained have shown that spermatozoa possessing higher levels of DNA protamination are more proficient in crossing the cervical mucus barrier. Moreover, the levels of positivity to endogenous nick translation treatment was practically zero in such spermatozoa.


CONCLUSIONS
We suggest that sperm penetration of cervical mucus could be used to select sperm preparations free of fragmented DNA or chromatin structural abnormalities for assisted reproduction.
Clinic of Sterility, Department of Obstetrics and Gynaecology, University Hospital of Geneva, Geneva, Switzerland.
P G
PG
Bianchi
A
A
De Agostini
J
J
Fournier
C
C
Guidetti
N
N
Tarozzi
D
D
Bizzaro
G C
GC
Manicardi
D002582:Cervix Mucus / Q000502:physiology*; D002843:Chromatin / Q000502:physiology*; D014128:Chromomycin A3 / Q000494:pharmacology; D004247:DNA / Q000502:physiology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D011897:Random Allocation; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology*
D002843:Chromatin; D005456:Fluorescent Dyes; D004247:DNA; D014128:Chromomycin A3