Crystallization and initial X-ray analysis of xylose isomerase from Thermotoga neapolitana.
Crystals of xylose isomerase from the hyperthermophile Thermotoga neapolitana have been grown using the microbatch method under oil from solutions containing Jeffamine ED 4000 as precipitant. The space group of the crystals is C222(1) with cell dimensions a = 161.8, b = 121.9, c = 98.9 A and they contain two monomers in the asymmetric unit. The crystals diffract beyond 2.7 A.
Blackett Laboratory, Imperial College of Science, Technology and Medicine, London, England. n.chayen@ic.ac.uk
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Chayen
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Conti
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Vieille
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JG
Zeikus