[Analytic study of dot blotting for the detection of anti-Jo-1, anti-M2, anti-ribosomes and anti-LKM].
The Cyto-Dot 4 HM043 kit commercialised by BMD, has replaced the Cyto-Dot HM010 kit that allowed three auto-antibodies detection (anti-Jo-1, anti-M2 and anti-ribosomal protein). Detection of anti-LKM1 auto-antibody was added. These four auto-antibodies have in common only the intracytoplasmic localisation of their respective antigen. The aim of our study was to evaluate this new kit using 104 sera and to compare our results with reference techniques (indirect immunofluorescence IF for anti-M2, anti-ribosomal protein and anti-LKM1, double immunodiffusion ID for anti-Jo-1 and anti-LKM1, western blotting WB for anti-M2) and with Cyto-Dot HM010. The one hundred and four sera were divided into five groups: Group I (n = 12) with anti-Jo-1 detected by ID; Group II (n = 28) with 26 anti-M2 positive by IF and WB, 2 anti-M2 positive only by WB; Group III (n = 10) with anti-ribosomal protein detected by IF 5 of which precipitated by ID; Group IV (n = 32) with anti-LKM1 by IF and ID divided into 18 AIH2 and 14 HCV; Group V (n = 22) consisting of 14 healthy individuals and 8 patients with hypergammaglobulinemia. Results of this study are similar to those of Cyto-Dot HM010 for the three auto-antibodies already in use. Cyto-Dot 4 is a very good anti-LKM1 confirmation method as it is ID.
Service d'immunologie et hématologie biologiques, Hôpital Saint-Antoine AP-HP, Paris.
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Huguet
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Sghiri
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Ballot
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Johanet
D001168:Arthritis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western / Q000592:standards; D017675:CREST Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D016022:Case-Control Studies; D003882:Dermatomyositis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D019084:Fluorescent Antibody Technique, Indirect / Q000592:standards; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D019693:Hepatitis, Autoimmune / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006643:Histidine-tRNA Ligase / Q000276:immunology*; D006801:Humans; D006942:Hypergammaglobulinemia / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015151:Immunoblotting / Q000379:methods* / Q000592:standards; D005779:Immunodiffusion / Q000592:standards; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D024101:Mitochondrial Proteins; D017285:Polymyositis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012270:Ribosomes / Q000276:immunology*; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D001324:Autoantigens; D024101:Mitochondrial Proteins; D011933:Reagent Kits, Diagnostic; C060729:anti-liver kidney microsome antibody; C052359:DLAT protein, human; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D006643:Histidine-tRNA Ligase