RNA interference for analysis of gene function in trypanosomatids.
Gene-specific silencing by RNA interference is a valuable tool for analysis of gene function in the protozoan parasite Trypanosoma brucei. The development of tetracycline-regulated vectors for production of double-stranded RNA has facilitated its widespread use. RNA interference provides a fast and efficient method for determining whether a gene is essential for growth and viability, reveals mechanistic information on gene function, and has greatly enhanced our understanding of complex biological processes. Finally, the creation of an RNA interference-based library has allowed, for the first time, an approach for conducting forward genetic experiments in this organism.
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA.
Shawn A
SA
Motyka
Paul T
PT
Englund
D000818:Animals; D020868:Gene Silencing; D017125:Genes, Protozoan; D005821:Genetic Techniques; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism
D015800:Protozoan Proteins