Apoptosis in right-ventricle biopsy is not predictive of graft survival.
Myocardial dysfunction is common in grafted hearts from brain-dead donors, but the mechanisms involved remain unclear, although apoptosis has been suggested to play an important role. In this study, we investigated the presence of apoptotic myocardial cells in donor hearts as compared to control hearts to determine whether pre-existing apoptosis can predict donor heart dysfunction. Apoptosis was studied by in situ DNA fragmentation assay and by Western Blotting for caspase-3, the pivotal executive caspase of the apoptotic pathway. We show that brain-death induced myocardial apoptosis was not predictive of myocardial dysfunction in transplanted hearts.
Department of Pathology, Hôpital Louis Pradel, BP Lyon Montchat 69 394, Cedex 03 Lyon, France. lara.chalabreysse@chu-lyon.fr
L
L
Chalabreysse
C
C
Leroux
J-F
JF
Obadia
T
T
Greenland
F
F
Thivolet-Bejui
J F
JF
Mornex
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D001926:Brain Death; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D006085:Graft Survival; D016027:Heart Transplantation; D006352:Heart Ventricles / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D011237:Predictive Value of Tests; D014019:Tissue Donors
C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases