Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes.
BACKGROUND
Preimplantation Genetic Diagnosis (PGD) using FISH to analyze up to nine chromosomes to discard chromosomally abnormal embryos has resulted in an increase of pregnancy rates in certain groups of patients. However, the number of chromosomes that can be analyzed is a clear limitation. We evaluate the reliability of using comparative genomic hybridization (CGH) to detect the whole set of chromosomes, as an alternative to PGD using FISH.


RESULTS
We have analysed by CGH both, first polar bodies (1PBs) and metaphase II (MII) oocytes from 30 oocytes donated by 24 women. The aneuploidy rate was 48%. Considering two maternal age groups, a higher number of chromosome abnormalities were detected in the older group of oocytes (23% versus 75%, P < 0.02). About 33% of the 1PB-MII oocyte doublets diagnosed as aneuploid by CGH would have been misdiagnosed as normal if FISH with nine chromosome probes had been used.


CONCLUSIONS
We demonstrate the reliability of 1PB analysis by CGH, to detect almost any chromosome abnormality in oocytes as well as unbalanced segregations of maternal translocations in a time frame compatible with regular in vitro fertilization (IVF). The selection of euploid oocytes could help to increase implantation and pregnancy rates of patients undergoing IVF treatment.
Gutiérrez-Mateo
Cristina
C
Departament de Biologia Cel.lular, Fisiologia i Immunologia, Unitat de Medicina, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain. cristinia.gutierrez@uab.es
Wells
Dagan
D
Benet
Jordi
J
Sánchez-García
Jorge F
JF
Bermúdez
Mercedes G
MG
Belil
Itziar
I
Egozcue
Josep
J
Munné
Santiago
S
Navarro
Joaquima
J
D000328:Adult; D000782:Aneuploidy; D016922:Cellular Senescence / Q000235:genetics; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008423:Maternal Age; D008677:Metaphase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D019836:Preimplantation Diagnosis / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D008941:Spindle Apparatus / Q000235:genetics*