RNA interference for analysis of gene function in trypanosomatids.
Gene-specific silencing by RNA interference is a valuable tool for analysis of gene function in the protozoan parasite Trypanosoma brucei. The development of tetracycline-regulated vectors for production of double-stranded RNA has facilitated its widespread use. RNA interference provides a fast and efficient method for determining whether a gene is essential for growth and viability, reveals mechanistic information on gene function, and has greatly enhanced our understanding of complex biological processes. Finally, the creation of an RNA interference-based library has allowed, for the first time, an approach for conducting forward genetic experiments in this organism.
Motyka
Shawn A
SA
Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA.
Englund
Paul T
PT
D000818:Animals; D020868:Gene Silencing; D017125:Genes, Protozoan; D005821:Genetic Techniques; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism
D015800:Protozoan Proteins