Alternative splicing of murine interleukin-4 mRNA.
We found an alternative form of mRNA with spliced second exon (IL-4delta2 mRNA) in mouse bone marrow and splenic cells. At rest, the amount of IL-4 mRNA markedly surpassed that of IL-4delta2 mRNA. Stimulation increased the content of both mRNA forms, but the alternative variant is accumulated more intensively and rapidly. We did not detect predominance of IL-4delta2 mRNA over full-length mRNA variant in the studied mouse tissues.
Yatsenko
O P
OP
Laboratory of Immunopoesis Regulation, Institute of Clinical Immunology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk.
Filipenko
M L
ML
Voronina
E N
EN
Khrapov
E A
EA
Sennikov
S V
SV
Kozlov
V A
VA
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014018:Tissue Distribution
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D015847:Interleukin-4