NMR analysis of enzyme-catalyzed and free-equilibrium mutarotation kinetics of monosaccharides.
We have exploited the saturation difference (SD) NMR technique to investigate the mutarotations of two sugars, l-fucose and d-ribose, at equilibrium. The KM and kcat values of Escherichia coli FucU-catalyzed mutarotation of l-fucose have been determined by this technique, and the values of KM for alpha- and beta-forms are shown to be the same. Similarly, the spontaneous mutarotation of d-ribose was investigated by using the same method, and it was found that d-ribose has an exceptionally high spontaneous alpha-to-beta conversion rate only between the furan forms.
Yusong-Gu, Gusong-Dong 373-1, Department of Chemistry and National Creative Research Institute Center, Korea Advanced Institute of Science and Technology, Daejon 305-701, South Korea.
Kyoung-Seok
KS
Ryu
Changhoon
C
Kim
Chankyu
C
Park
Byong-Seok
BS
Choi
D002236:Carbohydrate Conformation; D002238:Carbohydrate Epimerases / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D005663:Furans / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011714:Pyrans / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D005663:Furans; D011714:Pyrans; D005643:Fucose; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase