An antioxidant hispidin from the mycelial cultures of Phellinus linteus.
In the course of screening for reactive oxygen species scavengers from natural products, an antioxidant was isolated from the mycelial culture broth of Phellinus linteus and identified as hispidin. The hispidin content was reached its maximum level at 12 days after onset of inoculation. About 2.5 mg/mL of hispidin was produced by P. linteus in a yeast-malt medium (pH 5.8, 25 degrees C). Hispidin inhibited 22.6 and 56.8% of the super oxide anion radical, 79.4 and 95.3% of the hydroxyl radical, and 28.1 and 85.5% of the DPPH radical at 0.1 and 1.0 mM, respectively. The positive control alpha-tocopherol scavenged 25.6 and 60.3%, 74.6 and 96.3%, and 32.7 and 77.5% of each radical, respectively, at the same concentrations. However, hispidin showed no significant activity on the hydrogen peroxide radical.
Division of Applied Biology & Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, 1370, Sankyuk-Dong, Daegu 702-701, Korea.
In-Hye
IH
Park
Shin-Kyo
SK
Chung
Kyung-Bok
KB
Lee
Yung-Choon
YC
Yoo
Suk-Kyung
SK
Kim
Geum-Soog
GS
Kim
Kyung-Sik
KS
Song
D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry*; D010851:Picrates; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification; D013481:Superoxides / Q000737:chemistry; D013997:Time Factors
D000975:Antioxidants; D001713:Biphenyl Compounds; D006834:Hydrazines; D010851:Picrates; D011753:Pyrones; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C007946:hispidin