Supplemental diagnosis of Kudoa funduli (Myxozoa) parasitizing Fundulus heteroclitus (Cyprinodontidae) from coastal northeastern North America.
The diagnosis of Kudoa funduli (Hahn, 1915) Meglitsch, 1948 (Myxozoa), is supplemented through study of new material collected from Fundulus heteroclitus (Cyprinodontidae) in coastal waters of Nova Scotia, Canada, and Connecticut. Plasmodia normally develop intracellularly in striated muscle of the flank and head, eventually rupturing and releasing spores. Spores disperse along adjacent epimysium, sometimes as far as the skin surface. Some plasmodia develop extracellularly within the bony cavities of vertebrae. Formalin-fixed spores viewed with a light microscope possess rounded edges, an inconspicuous apical region, thin sutural ridges, measure 6.6-7.4 microm wide, 4.3-5 microm thick, and 5.1-5.4 microm long, and have 4 equally sized polar capsules, 1.7-2.3 microm length by 1.4-1.7 microm width. Scanning electron microscopy revealed that spores are almost stellate, with inconspicuous uplifted tips, and that, within intracellular plasmodia, are embedded in an extensive honeycomb-like matrix. Prevalence of infection of K. funduli was 100% in host populations sampled in both Nova Scotia and Connecticut. Molecular sequence data of the 18S ribosomal DNA (737 base pairs) reveal that K. funduli is a valid species and a member of a clade that includes Kudoa dianae Dyková, Avila, and Fiala, 2002, Kudoa miniauriculata Whitaker, Kent, and Sakanari, 1996, and Kudoa paniformis Kabata and Whitaker, 1981.
Departmento de Biologia Celular, Universidad Federal do Parana, Caixa Postal 19031, Brazil.
Fabiola
F
Akaishi
Russell
R
Easy
Sylvie
S
St-Jeans
Simon
S
Courtenay
Ciro A de Oliveira
CA
Ribeiro
David
D
Cone
D000818:Animals; D001483:Base Sequence; D003237:Connecticut / Q000453:epidemiology; D004275:DNA, Ribosomal / Q000737:chemistry; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D023781:Fundulidae / Q000469:parasitology*; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D008969:Molecular Sequence Data; D009674:Nova Scotia / Q000453:epidemiology; D010802:Phylogeny; D015995:Prevalence; D011529:Protozoan Infections, Animal / Q000453:epidemiology / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D033761:Spores, Protozoan / Q000648:ultrastructure
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S