Crystallization and preliminary X-ray study of a new crystal form of cytochrome c' from Rhodobacter capsulatus.
A new crystal form of diheme cytochrome c' from Rhodobacter capsulatus has been obtained and preliminary crystallographic experiments have been performed. The crystals belong to the space group P2(1)2(1)2 with unit-cell dimensions of a = 47.82, b = 72.59, c = 34.32 A. The assumption that an asymmetric unit of the crystal contains one half of the homodimer molecule indicates that the monomers in the dimeric molecule may be related by a crystallographic twofold axis. Crystals diffract up to 1.7 A resolution using the X-ray beam from synchrotron radiation, and 11 127 unique structure factors were obtained with an R(merge) of 7.1% from 52 922 indexed reflections. Structure analysis by means of molecular-replacement methods is now underway.
Higuchi
Y
Y
Department of Life Science, Himeji Institute of Technology, Hyogo, Japan.
Okama
Y
Y
Kanda
T
T
Misaki
S
S
Yasuoka
N
N
Meyer
T E
TE
Cusanovich
M A
MA