Detection and manipulation of biomolecules by magnetic carriers.
The detection and manipulation of single molecules on a common platform would be of great interest for basic research of biological or chemical systems. A promising approach is the application of magnetic carriers. The principles are demonstrated in this contribution. It is shown that paramagnetic beads can be detected by highly sensitive magnetoresistive sensors yielding a purely electronic signal. Different configurations are discussed. The capability of the sensors to detect even single markers is demonstrated by a model experiment. In addition, the paramagnetic beads can be used as carriers for biomolecules. They can be manipulated on-chip via currents running through specially designed line patterns. Thus, magnetic markers in combination with magnetoresistive sensors are a promising choice for future integrated lab-on-a-chip systems.
Department of Physics, University of Bielefeld, Universitätsstrasse 25, 33615 Bielefeld, Germany.
M
M
Brzeska
M
M
Panhorst
P B
PB
Kamp
J
J
Schotter
G
G
Reiss
A
A
Pühler
A
A
Becker
H
H
Brückl
D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods; D008280:Magnetics; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D014159:Transducers
D001704:Biopolymers