Optimization of the biodegradation of naphthalene by a microorganism isolated from petroleum contaminated soil.
Response surface methodology (RSM) was used to optimise the process parameters to obtain maximum biodegradation of naphthalene. The factorial design employed, a face-centred cube, helped in identifying the combined best parameter conditions. Naphthalene-containing medium was inoculated with Pseudomonas fluorescens and incubated at different levels of agitation, pH, and temperature, and analyzed by Gas Chromatography-Mass Spectroscopy. As a result of the optimisation process, the process parameters found for maximum degradation of naphthalene by P. fluorescens were an agitation speed of 186 rpm, a pH of 7.3, and a temperature of 22.8 degrees C.
Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada, A1B 3X9. amartin@mun.ca
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Martin
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Sivagurunathan
D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D007700:Kinetics; D009281:Naphthalenes / Q000493:pharmacokinetics*; D011551:Pseudomonas fluorescens / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
D009281:Naphthalenes; C031721:naphthalene