Escherichia coli serogroup O107/O117 lipopolysaccharide binds and neutralizes Shiga toxin 2.
The AB(5) toxin Shiga toxin 2 (Stx2) has been implicated as a major virulence factor of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains in the progression of intestinal disease to more severe systemic complications. Here, we demonstrate that supernatant from a normal E. coli isolate, FI-29, neutralizes the effect of Stx2, but not the related Stx1, on Vero cells. Biochemical characterization of the neutralizing activity identified the lipopolysaccharide (LPS) of FI-29, a serogroup O107/O117 strain, as the toxin-neutralizing component. LPSs from FI-29 as well as from type strains E. coli O107 and E. coli O117 were able bind Stx2 but not Stx1, indicating that the mechanism of toxin neutralization may involve inhibition of the interaction between Stx2 and the Gb(3) receptor on Vero cells.
Gamage
Shantini D
SD
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, OH 45267-0524 USA.
McGannon
Colleen M
CM
Weiss
Alison A
AA
D000818:Animals; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000737:chemistry*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D022622:Shiga Toxin 1 / Q000378:metabolism / Q000633:toxicity; D022641:Shiga Toxin 2 / Q000378:metabolism* / Q000633:toxicity; D014281:Trihexosylceramides / Q000378:metabolism; D014709:Vero Cells
D019081:O Antigens; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014281:Trihexosylceramides; C018549:globotriaosylceramide