Interactions of butyric acid- and acetic acid-treated Salmonella with chicken primary cecal epithelial cells in vitro.
In vitro studies of the interaction between pathogenic bacteria and the chicken intestinal epithelium are hampered by the lack of a host- and tissue-specific in vitro model. Therefore, a reproducible method for isolation and cultivation of chicken primary cecal epithelial cells was developed. Cecal crypts were isolated and cultured in vitro to form a semiconfluent layer of epithelial cells. Incubation of Salmonella enteritidis with these cells resulted in invasion. Pretreatment of the Salmonella bacteria with butyric acid resulted in a significant decrease of invasion of the bacteria in the chicken cecal epithelial cells, whereas pretreatment with acetic acid increased invasiveness. These interactions of S. enteritidis with primary chicken cecal epithelial cells were similar to the interactions with other epithelial cell types.
Van Immerseel
Filip
F
Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
De Buck
Jeroen
J
De Smet
Isabel
I
Pasmans
Frank
F
Haesebrouck
Freddy
F
Ducatelle
Richard
R
D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology*; D002432:Cecum / Q000382:microbiology*; D002478:Cells, Cultured; D002645:Chickens; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D007633:Keratins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D012477:Salmonella enteritidis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014746:Vimentin / Q000378:metabolism
D014746:Vimentin; D020148:Butyric Acid; D007633:Keratins; D019342:Acetic Acid