Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide.
Ligusticum chuanxiong and Angelica sinensis have been widely used in traditional Chinese medicine to treat some pathological settings such as atherosclerosis and hypertension. We determined the protective effect of the extract of Ligusticum chuanxiong and Angelica sinensis (ELCAS) on human umbilical vein endothelial cells (ECV304) damage induced by hydrogen peroxide. ECV304 cells were pre-treated with ELCAS and exposed to 5 mM hydrogen peroxide. The results show that ELCAS dose- and time-dependently protected ECV304 cells against hydrogen peroxide damage and suppressed the production of reactive oxygen species (ROS). The decrement of ROS may be associated with increased activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Western blot analysis revealed that ELCAS significantly increased the phosphorylation of ERK and promoted eNOS expression. These observations indicate that ELCAS protected ECV304 cells against hydrogen peroxide damage by enhancing the antioxidative ability, activating ERK and eNOS signaling pathway. Our data also provide new evidence of Ligusticum chuanxiong and Angelica sinensis in preventing both cardiovascular and cerebrovascular diseases.
Hou
Yong Zhong
YZ
P.O. Box 6888, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, 300072, PR China.
Zhao
Guang Rong
GR
Yang
Jie
J
Yuan
Ying Jin
YJ
Zhu
Guo Guang
GG
Hiltunen
Raimo
R
D000704:Analysis of Variance; D029971:Angelica sinensis / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002374:Catalase / Q000096:biosynthesis*; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D042783:Endothelial Cells / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D030004:Ligusticum; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D013997:Time Factors
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C101574:chuangxiong extract