Discovering protein-protein interactions.
The ongoing genomics and proteomics efforts have helped identify many new genes and proteins in living organisms. However, simply knowing the existence of genes and proteins does not tell us much about the biological processes in which they participate. Many major biological processes are controlled by protein interaction networks. A comprehensive description of protein-protein interactions is therefore necessary to understand the genetic program of life. In this tutorial, we provide an overview of the various current high-throughput methods for discovering protein-protein interactions, covering both the conventional experimental methods and new computational approaches.
Ng
See-Kiong
SK
Knowledge Discovery Department, Institute for Infocomm Research, 21 Heng Mui Keng Terrace, Singapore 119613, Singapore. skng@i2r.a-star.edu.sg
Tan
Soon-Heng
SH
D019677:Artificial Gene Fusion; D002846:Chromatography, Affinity; D019295:Computational Biology; D030562:Databases, Protein; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008954:Models, Biological; D019151:Peptide Library; D010802:Phylogeny; D040081:Protein Array Analysis / Q000706:statistics & numerical data; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000235:genetics; D020798:Two-Hybrid System Techniques / Q000706:statistics & numerical data
D046911:Macromolecular Substances; D019151:Peptide Library; D011506:Proteins; D012333:RNA, Messenger