Hematopoietic progenitor cell large volume leukapheresis (LVL) on the Fenwal Amicus blood separator.
A technique for large volume leukapheresis (LVL) for hematopoietic progenitor cell (HPC) collection using the Fenwal Amicus is presented. It was compared to standard collections (STD) with regard to CD34+ cell yields and cross-cellular content. Optimal cycle volumes and machine settings were evaluated for LVL procedures. A total of 68 patients underwent 80 HPC collection procedures. Because of differences in CD34+ cell yields associated with peripheral white blood cell counts (WBC), the comparison was divided into groups of 20 with WBC < or =35 x 10(9)/L (< or =35 K) and those >35 x 10(9)/L (>35 K). Baseline CD34+ cell counts (peripheral count when patient started HPC collection) were used (median 18-23 cells/microl). Significantly more whole blood (corrected for anticoagulant) was processed with LVL (LVL 20 l vs. STD 13.5 l). For < or =35 K, median CD34+ x 10(6), WBC x 10(9), RBC ml, Plt x 10(11) yields/collection were 183, 21.2, 14, 0.8, respectively, for STD vs. 307, 22.1, 11, 1.0, respectively, for LVL. For >35 K, median CD34+ x 10(6), WBC x 10(9), RBC ml, Plt x 10(11) yields/collection were 189, 32.7, 15, 1.4, respectively, for STD vs. 69, 40.8, 21, 1.3, respectively, for LVL. We have described a method of LVL using the Amicus that, in patients with pre-procedure WBC < or =35 x 10(9)/L, collects more CD34+ cells than a standard procedure with acceptable cross-cellular content. This method is not recommended when pre-procedure WBC counts are >35 x 10(9)/L.
Division of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
Edwin A
EA
Burgstaler
Alvaro A
AA
Pineda
Jeffrey L
JL
Winters
D018952:Antigens, CD34 / Q000096:biosynthesis; D001772:Blood Cell Count; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D006403:Hematologic Tests; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods*; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors
D018952:Antigens, CD34