Toward a robust model of packing and scale-up for chromatographic beds. 2. Flow packing.
We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical compression (consolidation) test is used, the model captures the primary phenomena of the scale-up process. However, moduli determined from flow-packing experiments improve the accuracy of the predictions and show that the apparent rigidity of chromatographic resins is lower for flow packing than for mechanical compression. Using a modulus from flow-packing experiments provided quantitative scale-up predictions of flow packing carried out in columns with diameters between 200 and 450 mm at different locations and by different operators.
Keener
Ronald N
RN
Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22904-4741, USA.
Maneval
James E
JE
Fernandez
Erik J
EJ
D008962:Models, Theoretical; D010316:Particle Size; D010539:Permeability; D010865:Pilot Projects; D012680:Sensitivity and Specificity