Crystallization and preliminary X-ray studies of plastocyanin from Silene expressed in E. coli.
Plastocyanin from Silene (SilPc) expressed in E. coli has been crystallized in a form suitable for X-ray diffraction analysis by a macroseeding method using ammonium sulfate as a precipitant in acetate buffer (pH = 5.5). These crystals belong to the trigonal space group P3(1)21 or P3(2)21 with lattice parameters a = b = 76.6, c = 65.5 A, indicating an asymmetric unit containing two plastocyanin molecules. The crystals diffracted to at least 2.0 A.
Li
C
C
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Suita, Japan.
Inoue
T
T
Gotowda
M
M
Hamada
K
K
Nishio
N
N
Hibino
T
T
Takabe
T
T
Kai
Y
Y