The effect of supplementation with fish oil during pregnancy on breast milk immunoglobulin A, soluble CD14, cytokine levels and fatty acid composition.
BACKGROUND
Breast milk contains many immunomodulatory factors (soluble CD14 (sCD14), IgA and cytokines) with the potential to influence infant immune development.


OBJECTIVE
To determine if changes in breast milk omega-3 polyunsaturated fatty acid (n-3 PUFA) composition as a result of maternal dietary fish oil supplementation during pregnancy can modify levels of these immunological parameters in breast milk.


METHODS
In a randomized controlled trial, 83 atopic women received either 4 g fish oil capsules (containing 3.7 g n-3 PUFA) (n = 40) or 4 g olive oil capsules (n = 43) from 20 weeks gestation until delivery. Breast milk was collected 3 days post-partum and fatty acids were analysed by gas liquid chromatography and IgA, sCD14 and cytokines (IL-5, IL-6, IL-10, TNF-alpha and IFN-gamma) were quantitated by ELISA or time resolved fluorescence (TRF).


RESULTS
Omega-3 docosahexaenoic acid (DHA; 22:6n-3) and eicosapentaenoic acid (EPA; 20:5n-3) levels were significantly higher (P < 0.001) in breast milk from women supplemented with fish oil (n = 33, DHA mean 1.15%, SD 0.47% and EPA mean 0.16%, SD 0.07%) than in samples from the control group (n = 40, DHA mean 0.50%, SD 0.17% and EPA mean 0.05%, SD 0.02%). Breast milk arachidonic acid (AA; 20:4n-6) levels were significantly lower (P = 0.045) in the fish oil group (mean 0.55%, SD 0.12%) compared with the control group (mean 0.61%, SD 0.14%). Breast milk IgA was positively correlated with DHA (P = 0.046) and 22:5n-3 (P = 0.003), but inversely correlated with linoleic acid (LA; 18:2n-6) (P=0.034). Levels of sCD14 were also positively correlated with 22:5n-3 (P=0.009). Cytokines involved in IgA synthesis (IL-10 and IL-6) were also significantly correlated with both IgA and n-3 PUFA levels, although there were no differences in the levels of breast milk IgA, sCD14 or cytokines between study groups.


CONCLUSIONS
Supplementation with fish oil during pregnancy significantly alters early post-partum breast milk fatty acid composition. omega-3 PUFA levels were positively associated with IgA and sCD14 levels, suggesting a relationship between fatty acid status and mucosal immune function.
School of Paediatrics and Child Health, University of Western Australia, Perth, Western Australia.
J A
JA
Dunstan
J
J
Roper
L
L
Mitoulas
P E
PE
Hartmann
K
K
Simmer
S L
SL
Prescott
D016009:Chi-Square Distribution; D016207:Cytokines / Q000032:analysis; D019587:Dietary Supplements; D004311:Double-Blind Method; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D039382:Maternal Nutritional Physiological Phenomena; D008895:Milk, Human / Q000276:immunology*; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology*
D016207:Cytokines; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007070:Immunoglobulin A; D018950:Lipopolysaccharide Receptors; D000069463:Olive Oil; D010938:Plant Oils