Comparative FISH analysis of distribution of B chromosome repetitive DNA in A an d B chromosomes in two subspecies of Podisma sapporensis (Orthoptera, Acrididae).
FISH analysis of B chromosome repetitive DNA distribution in A and B chromosomes of two subspecies of Podisma sapporensis (P. s. sapporensis and P. s. krylonensis) was performed. In the B chromosomes, C-positive regions contained homologous DNA repeats present also in some C-positive A chromosome regions. Most C-negative regions contained DNA repeats characteristic of A chromosome euchromatic regions. The two subspecies analyzed differed in the location of A chromosome regions enriched with repeats homologous to repeats of B chromosomes. The only common region enriched with these B chromosome repeats in both subspecies was the X chromosome pericentromeric region. The origin of B chromosomes in P. sapporensis is discussed.
Institutes of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk State University, Novosibirsk, Russia.
A G
AG
Bugrov
T V
TV
Karamysheva
D N
DN
Rubtsov
O V
OV
Andreenkova
N B
NB
Rubtsov
D000818:Animals; D002871:Chromosome Banding / Q000379:methods; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004625:Embryo, Nonmammalian; D022041:Euchromatin / Q000235:genetics; D005260:Female; D006110:Grasshoppers / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics
D022041:Euchromatin; D004247:DNA