Expression of mptC of Listeria monocytogenes induces sensitivity to class IIa bacteriocins in Lactococcus lactis.
Sensitivity to class IIa bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (PTS) permease, in Listeria monocytogenes. To assess the involvement of this protein complex in class IIa bacteriocin activity, the mptACD operon, encoding, was heterologously expressed in an insensitive species, namely Lactococcus lactis, using the NICE double plasmid system. Upon induction of the cloned operon, the recombinant Lc. lactis became sensitive to leucocin A. Pediocin PA-1 and enterocin A also showed inhibitory activity against Lc. lactis cultures expressing mptACD. Furthermore, the role of the three genes of the mptACD operon was investigated. Derivative plasmids containing various combinations of these three genes were made from the parental mptACD plasmid by divergent PCR. The results showed that expression of mptC alone is sufficient to confer sensitivity to class IIa bacteriocins in Lc. lactis.
Laboratory of Fundamental and Applied Microbiology, LCEE UMR CNRS 6008, University of Poitiers, 40, avenue du Recteur Pineau, 86022 Poitiers Cedex, France.|Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
Manilduth
M
Ramnath
Laboratory of Fundamental and Applied Microbiology, LCEE UMR CNRS 6008, University of Poitiers, 40, avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Safia
S
Arous
The Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Centre for Advanced Food Studies, LMC, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
Anne
A
Gravesen
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
John W
JW
Hastings
Laboratory of Fundamental and Applied Microbiology, LCEE UMR CNRS 6008, University of Poitiers, 40, avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Yann
Y
Héchard
D001430:Bacteriocins / Q000145:classification / Q000494:pharmacology*; D001483:Base Sequence; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000235:genetics*; D009561:Nisin / Q000494:pharmacology; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D001430:Bacteriocins; D004269:DNA, Bacterial; D021122:Protein Subunits; D011994:Recombinant Proteins; D009561:Nisin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase