Ethanol does not alter the binding of the gamma-hydroxybutyric acid (GHB) receptor ligand [3H]NCS-382 in the rat brain.
We investigated the effect of ethanol on the binding of the gamma-hydroxybutyric acid (GHB) receptor ligand [3H]NCS-382 in the rat cerebral cortex and hippocampus. Ethanol (50-100 mM) did not alter the binding of [3H]NCS-382. Furthermore, acute (3g/kg, p.o.) as well as chronic (9-15 g/kg/day p.o. for 6 days) administration of ethanol also did not have any significant effect on the binding of [3H]NCS-382 in the rat cerebrocortical and hippocampal membranes. These observations suggest that ethanol does not interact directly with the GHB receptor in vitro or in vivo, and GHB receptor may not be involved in the pharmacological effects of ethanol.
Department of Pharmacology, MC 7764, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio TX 78229-3900, USA.
Ashok K
AK
Mehta
Nicole M
NM
Muschaweck
Maharaj K
MK
Ticku
D000818:Animals; D001567:Benzocycloheptenes / Q000378:metabolism*; D001665:Binding Sites / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D008024:Ligands; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012978:Sodium Oxybate / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
C039403:4-hydroxybutyric acid receptor; D001567:Benzocycloheptenes; D008024:Ligands; D011956:Receptors, Cell Surface; D014316:Tritium; C066471:NCS 382; D000431:Ethanol; D012978:Sodium Oxybate