Alkali-stable high-pI isoelectric membranes for isoelectric trapping separations.
Alkali-stable, high-pI isoelectric membranes have been synthesized from quaternary ammonium derivatives of cyclodextrins and poly(vinyl alcohol), and bifunctional cross-linkers, such as glycerol-1,3-diglycidyl ether. The new, high-pI isoelectric membranes were successfully applied as cathodic membranes in isoelectric trapping separations in place of the hydrolytically more labile, polyacrylamide-based cathodic isoelectric membranes, and permitted the use of catholytes as alkaline as 1 M NaOH. The new high-pI isoelectric membranes have shown excellent mechanical stability, low electric resistance and long life times, even when subjected to electrophoresis with current densities as high as 80 mA/cm2.
Department of Chemistry, Texas A&M University, College Station, TX 778452-3012, USA.
Sanjiv
S
Lalwani
Evan
E
Shave
Helen C
HC
Fleisher
Kingsley
K
Nzeadibe
M Brent
MB
Busby
Gyula
G
Vigh
D003432:Cross-Linking Reagents / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004586:Electrophoresis / Q000295:instrumentation*; D008566:Membranes / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D011142:Polyvinyl Alcohol