Crystallization and preliminary X-ray crystallographic analysis of recombinant transaldolase B from Eschericha coli.
Recombinant transaldolase from Escherichia coli, an enzyme of the pentose phosphate pathway has been crystallized by the vapor-diffusion method using polyethylene glycol 6000 as precipitant. The crystals are orthorhombic, space group P2(1)2(1)2(1) with cell dimensions a = 68.9, b = 91.3 and c = 130.5 A and diffract to 2 A resolution on a conventional X-ray source. The asymmetric unit very likely contains two subunits, corresponding to a packing density of 2.9 A(3) Da(-1).
Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Sweden.
J
J
Jia
Y
Y
Lindqvist
G
G
Schneider
U
U
Schörken
H
H
Sahm
G A
GA
Sprenger