What can one learn from experiments about the elusive transition state?
We present the results of an exact analysis of a model energy landscape of a protein to clarify the idea of the transition state and the physical meaning of the phi values determined in protein engineering experiments. We benchmark our findings to various theoretical approaches proposed in the literature for the identification and characterization of the transition state.
Chang
Iksoo
I
Department of Physics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Cieplak
Marek
M
Banavar
Jayanth R
JR
Maritan
Amos
A
D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011336:Probability; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods; D017510:Protein Folding; D017433:Protein Structure, Secondary
D010455:Peptides