Evidence that HSP70 gene expression may be useful for assessing the cytocompatibility of dental biomaterials.
In the current studies, we examined the possibility of using HSP70 gene regulation as a cytocompatibility test for dental biomaterials. For this reason, we assessed the effects of three metal salts, HgCl2, CuSO4 and NiCl2 on HSP70 gene expression in HeLa S3 cells using real-time Taqman quantitative PCR. Incubation of the cells for 4 h in medium containing HgCl2 (20 or 40 microM), CuSO4 (157, 313, 625 or 1250 microM) or NiCl2 (5000 and 10000 microM) significantly induced HSP70 mRNA. The real-time Taqman quantitative PCR was able to detect HSP70 mRNA induction at 4-fold lower concentrations of HgCl2 and 8-fold lower concentrations of CuSO4 than the Neutral Red cell viability assay. These results indicate that real-time Taqman quantitative PCR, in combination with the monitoring of cell viability, may be a valuable tool for distinguishing between specific HSP70 mRNA induction and cytocompatibility of metals in dental biomaterials.
Hashimoto
Yoshiya
Y
Deaprtment of Biomaterials, Osaka Dental University, Kuzuhahanazono-cho Hirakata, Osaka, Japan. yoshiya@cc.osaka-dent.ac.jp
Ueda
Akihiro
A
Nakamura
Masaaki
M
D000704:Analysis of Variance; D002470:Cell Survival / Q000187:drug effects; D019327:Copper Sulfate / Q000633:toxicity*; D003764:Dental Materials / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008627:Mercuric Chloride / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis
D003764:Dental Materials; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D008627:Mercuric Chloride; C022838:nickel chloride; D009532:Nickel; D019327:Copper Sulfate