Genomic characterization of a slow/low maedi visna virus.
The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome were cloned and sequenced. This isolate has an overall similarity of approximately 80% with the K1514 Maedi Visna virus (MVV) and approximately 70% with the caprine arthritis encephalitis virus (CAEV) Co strain. Phylogenetic analysis based on SU and RT nucleotide sequences grouped P1OLV with previously reported ovine MVV. To determine the virus replication rate, sheep choroid plexus (SCP) and lung cells, macrophages (MØ), and goat synovial membrane (GSM) cells were inoculated with either P1OLV or with the lytic North American strain WLC-1. Viral RNA in culture supernatants was measured by one-tube real time quantitative RT-PCR. Significant differences were observed between the replication rates of the two viruses, with WLC-1 growing rapidly and to high levels in all the cells tested, while P1OLV replicated more slowly and to lower levels inducing persistent infections in lung and SCP cells. The U3 region of the LTR of P1OLV lacks the sequence repeats that are present in the LTRs of WLC-1 and MVV prototype K1514 and that contain additional binding sites for the AML(vis) transcriptional factor. To evaluate the contribution of LTR in the virus replication rate in vitro, we measured the basal activity of the promoter from P1OLV and WLC-1 in a luciferase-driven gene expression assay and lower levels of expression were achieved for P1OLV. The genetic and biological properties of P1OLV will be useful for the study of virus transcriptional factors and genes that may be responsible for the slow/low phenotype.
Laboratório Nacional de Investigação Veterinária, Dpt. Virologia, Estrada de Benfica 701, 1549-011 Lisboa, Portugal.
Sílvia C
SC
Barros
Fernanda
F
Ramos
Margarida
M
Duarte
Teresa
T
Fagulha
Benedita
B
Cruz
Miguel
M
Fevereiro
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000821:virology; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000821:virology*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology; D020079:Terminal Repeat Sequences / Q000235:genetics; D014779:Virus Replication; D014790:Visna-maedi virus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology