Mechanism-based inactivation of human leukocyte elastase via an enzyme-induced sulfonamide fragmentation process.
We describe herein the design and in vitro biochemical evaluation of a novel class of mechanism-based inhibitors of human leukocyte elastase (HLE) that inactivate the enzyme via an unprecedented enzyme-induced sulfonamide fragmentation cascade. The inhibitors incorporate in their structure an appropriately functionalized saccharin scaffold. Furthermore, the inactivation of the enzyme by these inhibitors was found to be time-dependent and to involve the active site. Biochemical, HPLC, and mass spectrometric studies show that the interaction of these inhibitors with HLE results in the formation of a stable acyl complex and is accompanied by the release of (L) phenylalanine methyl ester. The data are consistent with initial formation of a Michaelis-Menten complex and subsequent formation of a tetrahedral intermediate with the active site serine (Ser(195)). Collapse of the tetrahedral intermediate with tandem fragmentation results in the formation of a highly reactive conjugated sulfonyl imine which can either react with water to form a stable acyl enzyme and/or undergo a Michael addition reaction with an active site nucleophilic residue (His(57)). It is also demonstrated herein that this class of compounds can be used in the design of inhibitors of serine proteases having either a neutral or basic primary substrate specificity. Thus, the results suggest that these inhibitors constitute a potential general class of mechanism-based inhibitors of (chymo)trypsin-like serine proteases.
Wei
Liuqing
L
Department of Chemistry, Wichita State University, Wichita, KS 67260, USA.
Lai
Zhong
Z
Gan
Xiangdong
X
Alliston
Kevin R
KR
Zhong
Jiaying
J
Epp
Jeff B
JB
Tu
Juan
J
Perera
Asiri B
AB
Van Stipdonk
Michael
M
Groutas
William C
WC
D015195:Drug Design; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013379:Substrate Specificity; D013449:Sulfonamides / Q000737:chemistry*
D004791:Enzyme Inhibitors; D013449:Sulfonamides; D019272:Leukocyte Elastase