Influence of colloid, preservation medium and trimetazidine on renal medulla injury.
In organ transplantation, preservation injury is an important factor which could influence short-term and long-term graft outcome. The renal medulla is particularly sensitive to oxidant stress and ischemia-reperfusion injury (IRI). Using an autotransplant pig kidney model, we investigated renal function and medullary damage determined between day 1 and week 2 after 24- or 48-h cold storage in different preservation solutions: University of Wisconsin solution (UW), Hopital Edouard Herriot solution (a high Na+ version of UW), ECPEG (high Na+ preservation solution with PEG) and ICPEG (a high K+ version of ECPEG) with or without trimetazidine (TMZ). TMZ improved renal preservation and increased renal function when added in each preservation solution (particularly HEH and ECPEG). Medullary damage led to the early appearance of trimethylamine-N-oxide (TMAO) followed by 1H-NMR in urine and plasma. TMZ and ECPEG is the most efficient association to reduce medullary damage. This study clarifies the role of colloid and polarity solution and the role of mitochondrial protection by TMZ.
INSERM ERM 324, Rue de La Milétrie, CHU de Poitiers, 86000, Poitiers, France.
Carole
C
Doucet
Delphine
D
Dutheil
Isabelle
I
Petit
Keqiang
K
Zhang
Michel
M
Eugene
Guy
G
Touchard
Anne
A
Wahl
Francois
F
Seguin
Serge
S
Milinkevitch
Thierry
T
Hauet
Gerard
G
Mauco
D003102:Colloids; D003470:Culture Media; D007679:Kidney Medulla / Q000293:injuries*; D014292:Trimetazidine / Q000494:pharmacology*
D003102:Colloids; D003470:Culture Media; D014292:Trimetazidine