A comparative study on mouse MHC class I sequences detected in Schistosoma japonicum recovered from BALB/c (H-2d) and C57BL/6 (H-2b) mice.
The mouse major histocompatibility complex (MHC) class I sequence was detected in all the 8-week-old Schistosoma japonicum recovered from BALB/c (H-2d) and C57BL/6 (H-2b) mice by in situ polymerase chain reaction (in situ PCR). The signals of the mouse class I MHC sequence were observed in the nuclei of the mesenchymal and reproductive cells of 8-week-old S. japonicum. Furthermore, the class I MHC sequence was detected in each DNA extracted from S. japonicum cercariae maintained in BALB/c and C57BL/6 mice by nested PCR. To prove both horizontal and vertical transmission of this sequence in schistosomes, we have used cercariae obtained from parasites maintained in BALB/c mice to infect C57BL/6 and BALB/c mice, and vice versa. The MHC sequences from adult worms were compared to the cercarial MHC and host MHC sequences. Nucleotide sequence comparisons between adult worm DNA, host (H-2d and H-2b mice) DNA and cercarial DNA used for the infection suggested that the sequence of mouse class I MHC was incorporated into schistosome adults and inherited throughout their life-cycle.
Imase
Atsuko
A
Department of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan. imase@dokkyomed.ac.jp
Ohmae
Hiroshi
H
Iwamura
Yukio
Y
Kirinoki
Masashi
M
Matsuda
Hajime
H
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000032:analysis; D004195:Disease Models, Animal; D018562:Disease Transmission, Infectious / Q000662:veterinary; D022761:Gene Transfer, Horizontal / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics*; D006579:Heterozygote; D006790:Host-Parasite Interactions / Q000235:genetics; D017403:In Situ Hybridization; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000469:parasitology*; D008810:Mice, Inbred C57BL / Q000469:parasitology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics*; D012554:Schistosomiasis japonica / Q000235:genetics / Q000635:transmission* / Q000662:veterinary*; D013045:Species Specificity
D018509:DNA, Helminth