[Analysis of chromosome translocations involving MML by hybridization with an oligonucleotide microarray].
Identification of chromosome rearrangements is of importance for exact diagnosis, risk assessment, and therapy in blood malignancies. A new method was proposed for rapid and accurate identification of leukemia forms caused by chromosome rearrangements involving MLL (11q23). The method combines reverse transcription-multiplex PCR and hybridization with an oligonucleotide microarray. The microarray was designed to detect the five most common MLL rearrangements: t(4;11) MLL/AF4, t(9;11) MLL/AF9, t(11;19) MLL/ELL, t(11;19) MLL/ENL, and dup(11) MLL/MLL. With clinical specimens, the method was shown to efficiently identify the chromosome translocations in leukemia patients.
Mitiaeva
O N
ON
Nasedkina
T V
TV
Zharinov
V S
VS
Isaeva
E A
EA
Turygin
A Iu
AIu
Chupeeva
V V
VV
Kreĭndlin
E Ia
EIa
Mirzabekov
A D
AD
D001483:Base Sequence; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D002894:Chromosomes, Human, Pair 4; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D051788:Myeloid-Lymphoid Leukemia Protein; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011519:Proto-Oncogenes / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
D017931:DNA Primers; D004268:DNA-Binding Proteins; C497422:KMT2A protein, human; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase