New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology.
OBJECTIVE
To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).


METHODS
Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.


RESULTS
Two biomarkers with M(r) 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients, a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained.


CONCLUSIONS
These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression.
Zhu
Xiao-Dong
XD
Department of Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Zhongguancun Beiyitiao, Beijing 100080, China.
Zhang
Wei-Hua
WH
Li
Cheng-Lin
CL
Xu
Yang
Y
Liang
Wei-Jiang
WJ
Tien
Po
P
D015415:Biomarkers / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis; D002353:Carrier State; D015331:Cohort Studies; D003937:Diagnosis, Differential; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis*; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis; D008297:Male; D011237:Predictive Value of Tests; D040081:Protein Array Analysis / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D015415:Biomarkers