Reverse genetics systems for the generation of segmented negative-sense RNA viruses entirely from cloned cDNA.
Reverse genetics is defined as the generation of virus entirely from cloned cDNA. For negative-sense RNA viruses, whose genomes are complementary to mRNA in their orientation, the viral RNA(s) and the viral proteins required for replication and translation must be provided to initiate the viral replication cycle. Segmented negative-sense RNA viruses were refractory to genetic manipulation until 1989. In this chapter, we review developments in the reverse genetics of segmented negative-sense RNA viruses, beginning with the in vitro reconstitution of viral polymerase complexes in the late 1980s and culminating in the generation of Bunyamwera and influenza virus entirely from plasmid DNA almost a decade later.
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
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Neumann
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Kawaoka
D018076:DNA, Complementary / Q000235:genetics; D016296:Mutagenesis; D010957:Plasmids; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication
D018076:DNA, Complementary; D012367:RNA, Viral; D012319:RNA Polymerase II; D012318:RNA Polymerase I