[Degradation of 2,4-dinitrophenol by free and immobilized cells of Rhodococcus erythropolis HL PM-1].
Degradation of 2,4-dinitrophenol (2,4-DNP) by the cells of Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. Maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h-1. The reactor operated for 14 days without losing capacity; its half-lifetime equaled 16 days.
Pushchino State University, Pushchino, Moscow oblast, 142290 Russia.
A E
AE
Kitova
T N
TN
Kuvichkina
A Iu
AIu
Arinbasarova
A N
AN
Reshetilov
D019297:2,4-Dinitrophenol / Q000378:metabolism*; D000362:Agar; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology* / Q000592:standards; D005782:Gels; D006207:Half-Life; D012240:Rhodococcus / Q000378:metabolism*
D005782:Gels; D000362:Agar; D019297:2,4-Dinitrophenol