Preparation of dodecanol-tolerant strains of Yarrowia lipolytica.
Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strain MTLY35. These strains grew in rich media containing up to 10% (v/v) dodecanol. Dodecanol-tolerant strains remained dodecanol tolerant after they had been cultured in rich media without dodecanol. No significant amount of dodecanedioic acid was accumulated by the dodecanol-tolerant strains when grown on glucose in the presence of dodecanol.
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O Box 339, Bloemfontein 9300, South Africa. smitms.sci@mail.uovs.ac.za
Martha S
MS
Smit
Masego M
MM
Mokgoro
Evodia
E
Setati
Jean-Marc
JM
Nicaud
D042962:Acyl-CoA Oxidase / Q000172:deficiency* / Q000235:genetics; D000222:Adaptation, Physiological / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D007851:Dodecanol / Q000494:pharmacology*; D004361:Drug Tolerance / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D013045:Species Specificity; D025062:Yarrowia / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*
D007851:Dodecanol; D042962:Acyl-CoA Oxidase