Time-domain fluorescence lifetime imaging applied to biological tissue.
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue.
Elson
Dan
D
Photonics Group, Physics Department, Imperial College London, London, UK. ds.elson@imperial.ac.uk
Requejo-Isidro
Jose
J
Munro
Ian
I
Reavell
Fred
F
Siegel
Jan
J
Suhling
Klaus
K
Tadrous
Paul
P
Benninger
Richard
R
Lanigan
Peter
P
McGinty
James
J
Talbot
Clifford
C
Treanor
Bebhinn
B
Webb
Stephen
S
Sandison
Ann
A
Wallace
Andrew
A
Davis
Dan
D
Lever
John
J
Neil
Mark
M
Phillips
David
D
Stamp
Gordon
G
French
Paul
P
D000818:Animals; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods*; D004724:Endoscopy; D005453:Fluorescence; D006801:Humans; D008853:Microscopy