Autocatalytic self-propagation of misfolded prion protein.
Prions are thought to replicate in an autocatalytic process that converts cellular prion protein (PrP(C)) to the disease-associated misfolded PrP isoform (PrP(Sc)). Our study scrutinizes this hypothesis by in vitro protein misfolding cyclic amplification (PMCA). In serial transmission PMCA experiments, PrP(Sc) was inoculated into healthy hamster brain homogenate containing PrP(C). Misfolded PrP was amplified by rounds of sonication and incubation and reinoculated into fresh brain homogenate every 10 PMCA rounds. The amplification depended on PrP(C) substrate and could be inhibited by recombinant hamster PrP. In serial dilution experiments, newly formed misfolded and proteinase K-resistant PrP (PrPres) catalyzed the structural conversion of PrP(C) as efficiently as PrP(Sc) from brain of scrapie (263K)-infected hamsters, yielding an approximately 300-fold total amplification of PrPres after 100 rounds, which confirms an autocatalytic PrP-misfolding cascade as postulated by the prion hypothesis. PrPres formation was not paralleled by replication of biological infectivity, which appears to require factors additional to PrP-misfolding autocatalysis.
Bieschke
Jan
J
Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor-Lynen-Strasse 23, 81377 Munich, Germany. jbiesch@scripps.edu
Weber
Petra
P
Sarafoff
Nikolaus
N
Beekes
Michael
M
Giese
Armin
A
Kretzschmar
Hans
H
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002384:Catalysis; D006224:Cricetinae; D019286:Endopeptidase K / Q000378:metabolism; D066298:In Vitro Techniques; D008647:Mesocricetus; D008954:Models, Biological; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding
D018621:PrPC Proteins; D018620:PrPSc Proteins; D011328:Prions; D019286:Endopeptidase K