Identification of microcystin-RR and [Dha7]microcystin-RR in commercial standards by electrospray ionization mass spectrometry.
Three different commercial standards of microcystin-RR were assessed for purity by the liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI/MS) technique. Although the liquid chromatograms with photodiode array detector for each standard looked virtually identical, the analysis of corresponding mass spectra revealed that only one of them contained microcystin-RR per purity assay. The second standard was a mixture of microcystin-RR, and its demethyl variant identified as [Dha7]microcystin-RR, and the third one contained [Dha7]microcystin-RR only. We strongly recommend applying LC coupled with MS for purity assay of microcystin standards.
Kubwabo
Cariton
C
Health Canada, Safe Environments Programme, Chemistry Research Division, Environmental Health Centre, PL, Tunney's Pasture, Ottawa, Ontario, Canada. Cariton_kubwabo@hc-sc.gc.ca
Vais
Natalia
N
Benoit
Frank M
FM
D017033:Anabaena / Q000737:chemistry; D007202:Indicators and Reagents; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
C492352:((Dha)7)-microcystin-RR; D007202:Indicators and Reagents; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063855:microcystin RR