[Recombinant human BMP-2 accelerates bone formation of mandibular distraction osteogenesis in rabbits].
OBJECTIVE
To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits.


METHODS
Bilateral mandibular osteotomies were performed in 12 mature rabbits. 5 mg rhBMP-2 with the collagen carrier was implanted in the osteotomy site of one side of the mandibles. Only the collagen sponge was placed in the contra-lateral side as control. The mandibles of 8 rabbits were lengthened by 6mm using a custom-made distractor. At 4 weeks after the end of distraction, all animals were killed and the distracted calluses were harvested and processed for histological, scanning electron microscopic, as well as Ca/P ratio analysis.


RESULTS
The regenerated bone was found in the distraction gap after mandibular lengthening. The mandibular side treated with rhBMP-2 had greater amounts of new bone formation and earlier mineralization than contra-lateral side (non-rhBMP-2 treated).


CONCLUSIONS
Recombinant human BMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in rabbits.
Wang
Zhi-guo
ZG
Key. Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Hu
Jing
J
Zou
Shu-jian
SJ
Li
Ji-Hua
JH
Gao
Zhan-wei
ZW
Wang
Da-zhang
DZ
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D005260:Female; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis; D019857:Osteogenesis, Distraction; D011817:Rabbits; D011859:Radiography; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen