Protonmotive cooperativity in cytochrome c oxidase.
Cooperative linkage of solute binding at separate binding sites in allosteric proteins is an important functional attribute of soluble and membrane bound hemoproteins. Analysis of proton/electron coupling at the four redox centers, i.e. Cu(A), heme a, heme a(3) and Cu(B), in the purified bovine cytochrome c oxidase in the unliganded, CO-liganded and CN-liganded states is presented. These studies are based on direct measurement of scalar proton translocation associated with oxido-reduction of the metal centers and pH dependence of the midpoint potential of the redox centers. Heme a (and Cu(A)) exhibits a cooperative proton/electron linkage (Bohr effect). Bohr effect seems also to be associated with the oxygen-reduction chemistry at the heme a(3)-Cu(B) binuclear center. Data on electron transfer in cytochrome c oxidase are also presented, which, together with structural data, provide evidence showing the occurrence of direct electron transfer from Cu(A) to the binuclear center in addition to electron transfer via heme a. A survey of structural and functional data showing the essential role of cooperative proton/electron linkage at heme a in the proton pump of cytochrome c oxidase is presented. On the basis of this and related functional and structural information, variants for cooperative mechanisms in the proton pump of the oxidase are examined.
Papa
Sergio
S
Department of Biomedical Science, Faculty of Medicine, University of Foggia, Foggia, Italy. papabchm@cimedoc.uniba.it
Capitanio
Nazzareno
N
Capitanio
Giuseppe
G
Palese
Luigi L
LL
D000818:Animals; D001419:Bacteria; D001665:Binding Sites; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D006418:Heme / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D017494:Proton Pumps / Q000737:chemistry / Q000378:metabolism*; D018892:Proton-Motive Force
D017494:Proton Pumps; C027728:heme a; D006418:Heme; D003576:Electron Transport Complex IV