Ceftazidime determination in serum by high-pressure liquid chromatography.
A rapid and sensitive high-pressure liquid chromatographic method with simple sample preparation was developed for the quantitative analysis of the beta-lactam antibiotic ceftazidime (CAS 78439-06-2, Fortum). A good linear relationship was established between the peak area and the amount of ceftazidime injected over a concentration range of 1 to 200 microg/ml. The detection limit of the method was calculated to be 0.9 microg/ml. Stability was shown at 4 degrees C and at -196 degrees C for time periods of 2 h and 84 days, respectively.
Humbert
Till
T
University Hospital Mainz, Clinic of Anaesthesiology, Mainz, Germany. humbert@anaesthesie.klinik.uni-mainz.de
Rümelin
Andreas
A
Fauth
Ulrich
U
D002442:Ceftazidime / Q000097:blood*; D002511:Cephalosporins / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results
D002511:Cephalosporins; D002442:Ceftazidime