Cloning, characterization and expression of the polypeptide release factor gene, eRF1, of Blepharisma japonicum.
The polypeptide release factor gene, eRF1, of Blepharisma japonicum (Bj-eRF1) was cloned and sequenced. Its coding region was 1314 base pairs and encodes a protein of 437 amino acids. The cloned gene was expressed in Escherichia coli and the recombinant Bj-eRF1 polypeptide was purified by Ni2+-nitrilotriacetic acid agarose and Superose12 chromatography. Pull-down analysis showed that the recombinant Bj-eRF1 interacts with the heterologously-expressed release factor, eRF3C, of Euplotes octocarinatus.
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, P.R. China.
Wei
W
Wang
Bao-Feng
BF
Chai
Klaus
K
Heckmann
Ai-Hua
AH
Liang
D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010454:Peptide Termination Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013149:Spiroplasma / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial / Q000235:genetics
D010454:Peptide Termination Factors; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C087971:peptide-chain-release factor 3