[Screening of mimic epitopes of Trichinella spiralis antigen from phage 12-mer peptide library].
OBJECTIVE
To screen special mimic epitopes of Trichinella spiralis antigen from peptide library for exploring new diagnostic antigens.


METHODS
Ts-IgG purified from serum of trichinosis patients was used to screen the phage 12-mer peptide library for 5 rounds. 24 clones were picked out randomly to detect the immunoactivity. The sensitivity and specificity of the 6 clones (T1 - T6) whose A values were higher than others were tested by ELISA.


RESULTS
The sensitivity of the clones T1 - T6 was the same with larval antigen of Trichinella spiralis (TsA) (positive rate: 100%, P > 0.05), and there was no difference in specificity between T1 - T6 and TsA (negative rate: 0 - 40%, P > 0.05); T3 and T6 did not react with sera from patients of paragonimiasis, showing higher specificity than TsA (P < 0.05); T6 did not react with sera from patients of schistosomiasis, also showing higher specificity than TsA (P < 0.05).


CONCLUSIONS
The mimic antigenic epitopes of Trichinella spiralis have been successfully obtained by screening phage 12-mer peptide library.
Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hong
H
Pan
Chang-fu
CF
Jiang
Tian-qun
TQ
Li
Jia-hui
JH
Lei
Hong-bo
HB
Shi
Lan-ying
LY
Wei
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D019151:Peptide Library; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology
D000947:Antigens, Helminth; D000939:Epitopes; D019151:Peptide Library