[Preparation and identification of monoclonal antibodies against the Region II+ motif in circumsporozoite protein of Plasmodium falciparum].
OBJECTIVE
To develop and identify the monoclonal antibodies (McAbs) against Region II+ motif in circumsporozoite protein of Plasmodium falciparum.


METHODS
BALB/c mice were immunized with 12 peptides within Region II+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained.


RESULTS
ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II+. IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii.


CONCLUSIONS
McAbs obtained can probe the Region II+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.
Zhang
Rui-juan
RJ
Department of Etiological Biology, Second Military Medical University, Shanghai 200433, China.
Zhu
Huai-min
HM
Li
Xiang-yu
XY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan