3-nitro-3-deaza-2'-deoxyadenosine as a versatile photocleavable 2'-deoxyadenosine mimic.
A new photocleavable 2'-deoxyadenosine mimic, 3-nitro-3-deaza-2'-deoxyadenosine (NidA), was prepared and introduced into DNA fragments via its 6-O-trimethylphenyl precursor phophoramidite. Photocleavage of the resulting oligonucleotide is highly efficient in single and double strands. Hybridization properties of NidA are very similar to those of deoxyadenosine.
Crey-Desbiolles
Caroline
C
LEDSS, UMR5616 CNRS, ICMG FR-2607 - BP53, Université Joseph Fourier, 38041 Grenoble, France.
Lhomme
Jean
J
Dumy
Pascal
P
Kotera
Mitsuharu
M
D040761:Biomimetic Materials / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D003839:Deoxyadenosines / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry
C492391:3-nitro-3-deaza-2'-deoxyadenosine; D003839:Deoxyadenosines; D009841:Oligonucleotides; D004247:DNA; C058118:2'-deoxyadenosine