A comparison of the effects of oral inoculation with Rotashield and pentavalent reassortant rotavirus vaccine (WC3-PV) on suckling CB17scid mice.
The effects of oral inoculation into infant CB17(scid) mice of two reassortant rotavirus vaccines were compared. The vaccines were Rotashield and WC3-PV, a mixture of five reassortants (G1, G2, G3, G4 and P1; pentavalent reassortant vaccine). Control mice were inoculated with a placebo. At 6 days post-inoculation (p.i.), 8 of 13 (62 %; P<0.005) Rotashield-inoculated mice developed hepatitis and/or bile-duct obstruction compared with none of 11 mice given WC3-PV and none of 14 given placebo. In the Rotashield-inoculated mice, only serotype G3 rhesus rotavirus (RRV) was isolated from multiple sites, including intestine, liver, pancreas, spleen, blood and mesenteric lymph nodes. Recovery of RRV from Rotashield-inoculated mice followed a biphasic pattern. The two peaks of RRV recovery appeared to coincide firstly with replication in the intestine during days 1-3 p.i., and secondly with virus infection of the liver from days 10 to 15 p.i. WC3 reassortants of four different serotypes were detected only at day 1 p.i. in the intestine, liver, pancreas and blood cells from three WC3-PV-inoculated mouse pups. However, WC3-PV did not produce any hepatopathology. Rotashield and WC3-PV appeared to exhibit different biological activity in infant CB17(scid) mouse pups.
Department of Pediatrics, State University of New York at Buffalo, School of Medicine and Biomedical Sciences, Division of Infectious Diseases, The Women and Children's Hospital of Buffalo, Buffalo, NY 14222, USA.
Haiping
H
Qiao
Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
H Fred
HF
Clark
Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Matthew
M
DiVietro
Department of Pediatrics, State University of New York at Buffalo, School of Medicine and Biomedical Sciences, Division of Infectious Diseases, The Women and Children's Hospital of Buffalo, Buffalo, NY 14222, USA.
Marie
M
Riepenhoff-Talty
D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D000956:Antigens, Viral / Q000032:analysis; D002779:Cholestasis / Q000209:etiology; D003967:Diarrhea / Q000209:etiology; D006505:Hepatitis / Q000209:etiology; D051379:Mice; D016513:Mice, SCID; D016865:Reassortant Viruses / Q000276:immunology*; D012401:Rotavirus / Q000302:isolation & purification; D022243:Rotavirus Vaccines / Q000009:adverse effects*
D000956:Antigens, Viral; D022243:Rotavirus Vaccines