Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation.
Quantitative polymerase chain reaction (Q-PCR) allows for the accurate and reproducible determination of the amount of target DNA in a sample through the measurement of PCR product accumulation in "real time." This method determines starting target DNA quantity over a large assay dynamic range and requires no post-PCR sample manipulation. When used in combination with the method of chromatin immunoprecipitation (ChIP), the amount of protein binding to a specific region of DNA can be accurately and rapidly determined. A method for quantifying the presence of acetylated histones H3 and H4 on different regions of a target locus using Q-PCR after ChIP is described.
Irvine
Ryan A
RA
Department of Urology, University of Southern California, Los Angeles, CA, USA.
Hsieh
Chih-Lin
CL
D000107:Acetylation; D002843:Chromatin / Q000378:metabolism*; D006657:Histones; D016133:Polymerase Chain Reaction / Q000379:methods*; D011233:Precipitin Tests / Q000379:methods*
D002843:Chromatin; D006657:Histones