Automated carbohydrate profiling by capillary electrophoresis: a bioindustrial approach.
Automated, high-resolution, quantitative, high-throughput analysis of mono- and oligosaccharides, produced by enzymatic digestion of cellohexaose (model substrate) and lignocellulosic biomass, is demonstrated using high-performance capillary electrophoresis in conjunction with a single-step fluorophore labeling strategy for sensitive laser-induced fluorescence detection. Unattended batch sample processing from 96-well plates enabled reliable industrial-scale carbohydrate analysis. Excellent resolution of mono- and oligosaccharides was achieved under suppressed electroosmotic flow conditions, using either covalently or dynamically coated fused-silica capillary columns. The proposed approach readily supports the demands of bioindustrial operation environments with respect to high-throughput carbohydrate profiling.
Khandurina
Julia
J
Diversa Corp., San Diego, CA 92121, USA.
Blum
David L
DL
Stege
Justin T
JT
Guttman
András
A
D002241:Carbohydrates / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*
D002241:Carbohydrates; D005456:Fluorescent Dyes; D009844:Oligosaccharides; C099423:cellohexaose