Preliminary X-ray crystallographic analysis of human salivary cystatin.
Human salivary cystatin, a thiol proteinase inhibitor, has been implicated in potential antimicrobial and antiviral functions of saliva. A variant of human salivary cystatin SN expressed and purified in an Escherichia coli expression system lacking residues 12-16 near the N-terminus (Delta12-16) has been crystallized by the vapor-diffusion technique. The crystals are of the hexagonal space group P622 and have cell constants of a = 85.41, b = 85.41, c = 131.6 A, alpha = beta = 90, gamma = 120 degrees, and contain two molecules of molecular weight 13 500 per asymmetric unit. The crystals diffract up to a resolution of 2.2 A and are suitable for X-ray diffraction analysis.
Department of Oral Biology and Dental Research Institute, School of Dental Medicine, State University of New York at Buffalo, NY 14214, USA. subbu@crystall.sdm.buffalo.edu
N
N
Ramasubbu
T
T
Weaver
C C
CC
Tseng
L A
LA
Bobek
M J
MJ
Levine