Experimental methods for "in vivo" study of detruso-sphincteric pharmacological response: a critical review.
OBJECTIVE
We review "in vivo" methods most commonly used for the investigation of detruso-sphincteric pharmacological response. We compare this information with the procedures used in our Institution for these purposes.


METHODS
The medical databases MEDLINE, EMBASE and Pascal Biomed were searched to identify articles on this subject. The methods used have been critically analyzed and compared with the methods used in our experiments.


RESULTS
The "in vivo" dynamic investigation of vesico-sphincteric function began at our Institution in the late 70s. Methods for the study of vesical or urethral dynamic behaviour have been devised to be applied independently or simultaneously. Great difficulties have been encountered, both in our experience and in the work of revised authors, in the integrated investigation of Lower Urinary Tract function. Methods to overcome these drawbacks have been proposed.


CONCLUSIONS
Methods for "in vivo" studies of physiological and pharmacological detruso-sphincteric function are presented and compared with current procedures found in the literature. It is highlighted that an integrated method for the simultaneous study of vesico-sphincteric function is a difficult challenge yet to be taken up.
Cátedra y Servicio de Urología, Fundación Jiménez Díaz, Universidad Autónoma de Madrid, Madrid, Spain. r.vela@fjd.es
Remigio
R
Vela Navarrete
Francisco Carlos
FC
Pérez Martínez
Javier
J
Cabrera Pérez
María
M
Ramírez Pérez del Yerro
Carmen
C
González Enguita
Carlos
C
Castilla Reparaz
D000818:Animals; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D014521:Urethra / Q000187:drug effects* / Q000502:physiology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology