Automated monitoring of phosphatidylcholine biosyntheses in Plasmodium falciparum by electrospray ionization mass spectrometry through stable isotope labeling experiments.
The metabolic pathways contributing to phosphatidylcholine biosyntheses in Plasmodium falciparum, the malaria-causing parasite, was explored by electrospray ionization mass spectrometry. Phosphatidylcholine produced by the CDP-choline pathway and by the methylation of phosphatidylethanolamine was identified and quantified through isotopic labeling experiments. A straightforward method based on cone voltage directed in-source fragmentations and relative abundance measurement of endogenous versus deuterated specific fragment ions was developed for simple and rapid automated data acquisition. Such high-throughput analytical protocol allowed us to measure the relative contribution of two different metabolic pathways leading to phosphatidylcholine without performing technically more demanding and time-consuming MS/MS or LC/MS experiments.
Enjalbal
Christine
C
UMR 5810, Laboratoire des Aminoacides Peptides et Protéines, Université Montpellier 2, Place E. Bataillon, 34095 Montpellier Cedex 05, France. enjalbal@univ-montp2.fr
Roggero
Rodolphe
R
Cerdan
Rachel
R
Martinez
Jean
J
Vial
Henri
H
Aubagnac
Jean-Louis
JL
D000818:Animals; D001331:Automation / Q000379:methods; D002853:Chromatography, Liquid; D003903:Deuterium; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010963:Plasmodium falciparum / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D010713:Phosphatidylcholines; D003903:Deuterium