3-D morphological characterization of the liver parenchyma by atomic force microscopy and by scanning electron microscopy.
A comparative study of atomic force microscopy (AFM) and scanning electron microscopy (SEM) imaging of the healthy human liver parenchyma was carried out to determine the similarities and the differences. In this study, we compared the fine hepatic structures as observed by SEM and AFM. Although AFM revealed such typical hepatic structures as bile canaliculi and hepatocytes, it also showed the location of the nucleus and chromatin granules in rough relief structure, which was not visible by SEM. By contrast, SEM visualized other structures, such as microvilli, the central vein, and collagenous fibers, none of which was visualized by AFM. For better orientation and confirmation of most of the structures imaged by SEM and AFM, Congo Red-stained specimens were also examined. Amyloid deposits in the Disse's spaces were shown especially clearly in these images. The differences between the SEM and AFM images reflected the characteristics of the detection systems and methods used for sample preparation. Our results reveal that more detailed information on hepatic morphology is obtained by exploiting the advantages of both SEM and AFM.
First Department of Anatomy, University of Vienna, A-1170 Vienna, Austria. mahmoud.melling@univie.ac.at
Mahmoud
M
Melling
Daniela
D
Karimian-Teherani
Sascha
S
Mostler
Mark
M
Behnam
Sonja
S
Hochmeister
D000328:Adult; D000682:Amyloid / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D022781:Hepatocytes / Q000648:ultrastructure*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D018625:Microscopy, Atomic Force / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D016707:Tissue Fixation / Q000379:methods
D000682:Amyloid