A 50-kDa isoform of mouse brain acyl-CoA hydrolase: expression and molecular properties.
Brain acyl-CoA hydrolase (BACH) is responsible for most of the long-chain acyl-CoA hydrolyzing activity in the brain and is localized exclusively in neurons. There are two BACH isoforms: the major isoform, a 43-kDa BACH, and a lesser isoform, a 50-kDa BACH. In our previous work [Brain Res. Mol. Brain Res. 98 (2002) 81], a possibility was raised that these BACH isoforms might be generated from a single mRNA species via a mechanism of alternative use of translation start sites. However, the results obtained in the current study indicated that the 43-kDa BACH and 50-kDa BACH are not generated from a single mRNA species, but from distinct mRNA species transcribed by alternative use of transcription start sites. The molecular properties of the 50-kDa BACH were compared to those of the 43-kDa BACH. Palmitoyl-CoA hydrolase activity and protein stability were almost the same between both BACH isoforms. In addition, both 43-kDa BACH and 50-kDa BACH that were fused to green fluorescent protein showed cytosolic distribution. These results suggest that the 50-kDa BACH plays a similar role as the 43-kDa BACH. Therefore, since the 43-kDa BACH is expressed at higher levels than 50-kDa BACH, the 43-kDa BACH should largely contribute to understanding the physiological functions of the BACH gene in neurons.
Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan. takagim@ps.toyaku.ac.jp
Mitsuhiro
M
Takagi
Keitaro
K
Kawabe
Tetsuya
T
Suga
Junji
J
Yamada
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D001923:Brain Chemistry; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D015972:Gene Expression Regulation, Neoplastic; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009447:Neuroblastoma / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D010170:Palmitoyl-CoA Hydrolase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017385:Sequence Homology; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D010170:Palmitoyl-CoA Hydrolase