Tat cell-penetrating peptide has the characteristics of a poly(proline) II helix in aqueous solution and in SDS micelles.
Tat cell-penetrating peptide (GRKKRRQRRRPPQG) is able to translocate and carry molecules across cell membranes. Using CD spectroscopy the conformation of this synthetic peptide was studied in aqueous and membrane-mimicking, micellar SDS solutions at different temperatures. The CD spectrum of the Tat cell-penetrating peptide in SDS micellar solution was virtually unchanged from that in aqueous solution, and at low temperature it was close to that of a poly(proline) II helix.
Ruzza
Paolo
P
Institute of Biomolecular Chemistry, Padova Unit, CNR, 35131 Padova, Italy. paolo.ruzza@unipd.it
Calderan
Andrea
A
Guiotto
Andrea
A
Osler
Alessio
A
Borin
Gianfranco
G
D002942:Circular Dichroism; D015696:Gene Products, tat / Q000737:chemistry* / Q000378:metabolism*; D008823:Micelles; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D015696:Gene Products, tat; D008823:Micelles; D010455:Peptides; D012996:Solutions; D014867:Water; C011083:polyproline; D012967:Sodium Dodecyl Sulfate