Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.
A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.
Gurumurthy
Prema
P
Biochemistry Department, Tuberculosis Research Centre (Indian Council of Medical Research), Chennai 600 031, India. icmrtrc@vsnl.com
Gayathri
T N
TN
Bhagavathy
S
S
Venkatesan
P
P
D000995:Antitubercular Agents / Q000493:pharmacokinetics* / Q000652:urine*; D002624:Chemistry, Clinical / Q000379:methods*; D002845:Chromatography / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004977:Ethambutol / Q000493:pharmacokinetics* / Q000652:urine*; D006679:HIV Seropositivity / Q000150:complications / Q000188:drug therapy; D006801:Humans; D007538:Isoniazid / Q000737:chemistry; D004364:Pharmaceutical Preparations; D011718:Pyrazinamide / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
D000995:Antitubercular Agents; D004364:Pharmaceutical Preparations; D012117:Resins, Synthetic; D011718:Pyrazinamide; D004977:Ethambutol; C046797:amberlite; D007538:Isoniazid