Assessment of the potential irritation and photoirritation of novel amino acid-based surfactants by in vitro methods as alternative to the animal tests.
The ultraviolet-A radiation damage effects on skin and eyes will be increased by phototoxic compounds which could be present in pharmaceutical or cosmetic formulations. Great efforts have been made in the last years to find surfactants to replace those with phototoxic potential in commercial use. Series of different in vitro models for phototoxicity, included to validated neutral red uptake (NRU) 3T3 phototoxicity assay are useful screening tools. The phototoxic effects of a novel family of glycerol amino acid-based surfactant compounds were examined via these assays. Human red blood cells and two immortalised cell lines, murine fibroblast cell line 3T3, and one human keratinocyte cell line, HaCaT, were the in vitro models employed to predict potential photoirritation. The phototoxic end-points assessed were hemolysis (human red blood cell test) and resazurin transformation to resorufin and NRU in cell culture methods. The results suggest that no phototoxic effects by any new amino acid derived-surfactants, could be identified.
Benavides
Tomas
T
Departament Fisiologia-Farmàcia, Facultat de Farmàcia, Universitat de Barcelona (BCN), Avinguda Joan XXIII s/N, 08028 Barcelona, Spain.
Martínez
Verónica
V
Mitjans
Montserrat
M
Infante
María Rosa
MR
Moran
Carmen
C
Clapés
Pere
P
Clothier
Richard
R
Vinardell
María Pilar
MP
D000596:Amino Acids / Q000494:pharmacology; D000826:Animal Testing Alternatives; D000818:Animals; D001769:Blood / Q000187:drug effects; D002478:Cells, Cultured; D017484:Dermatitis, Phototoxic; D005347:Fibroblasts / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007509:Irritants / Q000009:adverse effects; D051379:Mice; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000009:adverse effects*
D000596:Amino Acids; D007509:Irritants; D013501:Surface-Active Agents; D005990:Glycerol