Latanoprost eye drops increase concentration of glycosaminoglycans in posterior rabbit sclera.
OBJECTIVE
Glycosaminoglycans are important components of ocular tissues such as the sclera. The pressure reducing effect of a new antiglaucoma drug, latanoprost, is based on an increase in the uveo-scleral outflow by way of modulation of the intracellular matrix of the ciliary body. The purpose of the study was to test the effect of latanoprost on the content of glycosaminoglycans in rabbit cornea and sclera.


METHODS
Twelve rabbits were studied. Six rabbits were treated for 12 weeks with latanoprost eye drops and 6 with hydroxypropylmethylcellulose, dextran 70 eye drops for control. Samples were taken from cornea and anterior, lateral, and posterior sclera. Glycosaminoglycans were determined quantitatively by spectrophotometry (uronic acids).


RESULTS
A significant increase in the concentration of uronic acids was found in all three localisations of sclera from latanoprost-treated animals. The increase was 26%, 24%, and 20% in anterior, lateral, and posterior sclera, respectively.


CONCLUSIONS
Long-term treatment with latanoprost induces biochemical changes in sclera. The results indicate that topically applied latanoprost reaches the posterior parts of the rabbit eye.
Trier Eye Clinic and Research Laboratories, Hellerup, Denmark. ktrier@dadlnet.dk
Klaus
K
Trier
Søren Munk
SM
Ribel-Madsen
D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D007322:Instillation, Drug; D000077338:Latanoprost; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D011817:Rabbits; D012590:Sclera / Q000187:drug effects* / Q000378:metabolism; D013053:Spectrophotometry; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic; D014574:Uronic Acids; C020341:glucosaminoglycans; D000077338:Latanoprost