Site-specific vibrational dynamics of the CD3zeta membrane peptide using heterodyned two-dimensional infrared photon echo spectroscopy.
Heterodyned two-dimensional infrared (2D IR) spectroscopy has been used to study the amide I vibrational dynamics of a 27-residue peptide in lipid vesicles that encompasses the transmembrane domain of the T-cell receptor CD3zeta. Using 1-(13)C[Double Bond](18)O isotope labeling, the amide I mode of the 49-Leucine residue was spectroscopically isolated and the homogeneous and inhomogeneous linewidths of this mode were measured by fitting the 2D IR spectrum collected with a photon echo pulse sequence. The pure dephasing and inhomogeneous linewidths are 2 and 32 cm(-1), respectively. The population relaxation time of the amide I band was measured with a transient grating, and it contributes 9 cm(-1) to the linewidth. Comparison of the 49-Leucine amide I mode and the amide I band of the entire CD3zeta peptide reveals that the vibrational dynamics are not uniform along the length of the peptide. Possible origins for the large amount of inhomogeneity present at the 49-Leucine site are discussed.
Mukherjee
Prabuddha
P
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Krummel
Amber T
AT
Fulmer
Eric C
EC
Kass
Itamar
I
Arkin
Isaiah T
IT
Zanni
Martin T
MT
D017252:CD3 Complex / Q000032:analysis* / Q000737:chemistry*; D003198:Computer Simulation; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D017785:Photons; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014732:Vibration
D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D008051:Lipid Bilayers; D008081:Liposomes; D008565:Membrane Proteins; D010455:Peptides