Paradoxical effects of elastase inhibitor guamerin on the tissue repair of two different wound models: sealed cutaneous and exposed tongue wounds.
Innate elastase inhibitors are known to be putatively involved in the regulation of tissue inflammation by inhibiting polymorphonuclear leukocyte (PMN) derived proteinases. The aim of this study was to evaluate affects of leukocyte elastase suppression and PMN infiltration on wound healing in mouse by administering the recombinant elastase inhibitor guamerin (rEIG) in two different wound models; 1) impaired pin-punctured dorsal mucosa of anterior tongue wound, 60 mice, treated with saline containing rEIG that were fed ad libitum and 2) stable linear excisional cutaneous wound, 40 mice, covered with fibrin sealant containing rEIG. The progress of healing was analyzed by histological methods. The tongue wounds treated with rEIG became edematous around the pin-punctured tongue wound, and influx of inflammatory cells and PMN into the underlying stromal tissue were seen rapidly after wounding and peaked between 2-4 days. Whereas the control mice showed almost no wheal formation in the pin-punctured wound, a far lesser levels of PMN infiltration, and almost complete wound closure in 4 days. In the other model, the liner excisional cutaneous wound treated with fibrin sealant containing rEIG showed early wound constriction, lesser degree of inflammatory cells influx, and complete reepithelialization in 4-5 days, whereas the wound of control mice with the fibrin sealant alone showed contrary delayed reepithelialization, greater degree of inflammatory cell infiltration, and consequencial formation of greater granulation tissue at wound site. Taken together, these data suggest paradoxical effects of rEIG on the wound healing where in the wound exposed to infiltrating milieu of microorganisms in the oral cavity, the rEIG aggravates the wound healing by interfering with other innate defensive factors and extended greater flux of PMNs to inflamed wound site, while in the wound enclosed by fibrin, the rEIG accelerated wound healing by inhibiting the inflammation-generated proteases and the acute inflammatory reaction.
Department of Oral Pathology, College of Dentistry, Kangnung National University, Gangneung, Korea.
Suk Keun
SK
Lee
Sang Shin
SS
Lee
In Sun
IS
Song
Yeon Sook
YS
Kim
Young Wook
YW
Park
Jae Yong
JY
Joo
Heung Sik
HS
Um
Jin Woo
JW
Kim
Kyeong Yeon
KY
Kim
Seung Jin
SJ
Choi
Kyung-Hwan
KH
Jung
Soo Il
SI
Chung
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D007447:Invertebrate Hormones / Q000032:analysis / Q000493:pharmacokinetics / Q000494:pharmacology*; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D014059:Tongue / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
D004791:Enzyme Inhibitors; D015718:Fibrin Tissue Adhesive; D007447:Invertebrate Hormones; C093975:guamerin; D019272:Leukocyte Elastase