Staurosporine restores GTPgammaS induced block of rapid endocytosis in chromaffin cells.
Fast capacitance measurements demonstrated that chromaffin cells retrieve membrane by several kinetically different pathways. Here, we show that rapid endocytosis is blocked and slow endocytosis reduced by intracellular application of GTPgammaS, an activator of G-proteins, but not by the competitive blocker GDPbetaS. The inhibition of rapid endocytosis by GTPgammaS can be restored with GDPbetaS or staurosporine completely. But only staurosporine partially abolishes the reduction of slow endocytosis by GTPgammaS. Besides triggering exocytosis, GTPgammaS elicits large exo- and endocytotic vesicles that contributed significantly to the total membrane traffic, indicating a third pathway of membrane shuttle.
Department of Psychiatry (Molecular Neurobiology), University of Erlangen-Nuremberg, Schwabachanlage 6, 91054 Erlangen, Germany. andreas.henkel@psych.imed.uni-erlangen.de
Andreas Wolfram
AW
Henkel
Tobias Kristian
TK
Vogt
Maria Kerstin
MK
Henkel
D000313:Adrenal Medulla; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D007700:Kinetics; D019311:Staurosporine / Q000494:pharmacology*
D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019311:Staurosporine