Serum P-selectin level during controlled ovarian hyperstimulation--a preliminary report.
OBJECTIVE
To measure levels of serum P-selectin in patients undergoing controlled ovarian hyperstimulation (COH) cycles and to determine their possible correlation to COH variables.


METHODS
Large university-based infertility and in vitro fertilization unit.


METHODS
Fourteen consecutive patients undergoing our routine COH protocol for unexplained infertility.


METHODS
Blood was drawn three times during the COH cycle: (1) day 2 or 3 of the menstrual cycle, before gonadotropin treatment (Day-0); (2) day of or prior to human chorionic gonadotropin (hCG) administration (Day-hCG); and (3) day of ovulation (Day-OVU). Serum levels of sex steroids and P-selectin were compared among the three time points. P-selectin was measured with a commercial quantitative sandwich immunoassay technique. To reduce interpatient variability, the percent difference between the Day-0 (non-stimulated, basal) level and the Day-hCG and Day-OVU levels was calculated.


RESULTS
P-selectin level on Day-hCG was significantly higher than on Day-0 (P < 0.05) and non-significantly higher than on Day-OVU (P < 0.12). No significant correlations were observed between serum P-selectin and patient age, amount of gonadotropins used, or estradiol or progesterone level.


CONCLUSIONS
The increase in serum P-selectin level during COH until peak estradiol suggests that COH may potentiate a state of platelet activation which is substantially attenuated after hCG administration.
Orvieto
Raoul
R
Department of Obstetrics and Gynecology, Rabin Medical Center, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. orvieto@clalit.org.il
Badir
Walid
W
Bar
Jacob
J
La Marca
Antonio
A
Ashkenazi
Jacob
J
Avrech
Ori M
OM
Fisch
Benjamin
B
D000328:Adult; D015232:Dinoprostone / Q000097:blood; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D016471:Ovarian Hyperstimulation Syndrome / Q000139:chemically induced / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D019007:P-Selectin / Q000097:blood*; D011374:Progesterone / Q000097:blood
D006062:Gonadotropins; D019007:P-Selectin; D011374:Progesterone; D015232:Dinoprostone