Tissue anti-adhesion potential of ibuprofen-loaded PLLA-PEG diblock copolymer films.
This study was designed to evaluate the effect of polyethylene glycol (PEG) and nonsteroidal anti-inflammatory drug (ibuprofen) on the prevention of postsurgical tissue adhesion. For this, poly(L-lactic acid) (PLLA)-PEG diblock copolymers were synthesized by ring opening polymerization of L-lactide and methoxy polyethylene glycol (Mw 5000) of different compositions. The synthesized copolymers were characterized by gel permeation chromatography and 1H-nuclear magnetic resonance spectroscopy. PLLA-PEG copolymer films were prepared by solvent casting. The prepared copolymer films were more flexible and hydrophilic than the control PLLA film, as investigated by the measurements of glass transition temperature, water absorption content, and water contact angle. The drug release behavior from the ibuprofen (10 wt%)-loaded copolymer films was examined by high performance liquid chromatography. It was observed that the drug was released gradually up to about 40% of total loading amount after 20 days, depending on PEG composition; more drug release from the films with higher PEG compositions. In vitro cell adhesions on the copolymer films with/without drug were compared by the culture of NIH/3T3 mouse embryo fibroblasts on the surfaces. For in vivo evaluation of tissue anti-adhesion potential, the copolymer films with/without drug were implanted between the cecum and peritoneal wall defects of rats and their tissue adhesion extents were compared. It was observed that the ibuprofen-containing PLLA-PEG films with high PEG composition (particularly PLLA113-PEG113 film with PEG composition, 50 mol%) were very effective in preventing cell or tissue adhesion on the film surfaces, probably owing to the synergistic effects of highly mobile, hydrophilic PEG and anti-inflammatory drug, ibuprofen.
Department of Polymer Science and Engineering, Hannam University, 133 Ojeong Dong, Daedeog Gu, Daejeon 306-791, Republic of Korea. jhlee@hannam.ac.kr
Jin Ho
JH
Lee
Ae Kyung
AK
Go
Se Heang
SH
Oh
Ka Eul
KE
Lee
Soon Hong
SH
Yuk
D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery; D020341:Absorbable Implants; D000042:Absorption; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002432:Cecum / Q000473:pathology / Q000601:surgery; D002448:Cell Adhesion / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical; D004343:Drug Implants; D004357:Drug Synergism; D007052:Ibuprofen / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D007773:Lactates / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D010537:Peritoneum / Q000473:pathology / Q000601:surgery; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D000267:Tissue Adhesions / Q000517:prevention & control*; D017155:Wettability
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D004343:Drug Implants; D007773:Lactates; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D007052:Ibuprofen