Antifungal constituents of Clytostoma ramentaceum and Mansoa hirsuta.
Ethanol extracts of Clytostoma ramentaceum Bur. & K. Schum and Mansoa hirsuta DC. (Bignoniaceae) inhibited the growth of standardized cultures of Aspergillus niger and Fusarium oxysporum, at concentrations of 400 microg and 500 microg, in bioautographic assays. The activity-guided fractionation of C. ramentaceum extract afforded ursolic acid and 2-(3',4'-dihydroxyphenyl) ethanol, both active against the test fungi (100 microg). These compounds are reported for the first time in C. ramentaceum and were not detected in M. hirsuta extract, according to HPLC analysis. The bioguided study of M. hirsuta resulted in five active fractions (100 to 200 microg), whose GC-MS analysis allowed us to identify 11 compounds, mostly alkanols and alkanodiols, that may be regarded as the antifungal constituents of M. hirsuta.
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Alessandra Duarte
AD
Rocha
Alaíde Braga
AB
de Oliveira
José Dias
JD
de Souza Filho
Júlio Antônio
JA
Lombardi
Fernão Castro
FC
Braga
D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001234:Aspergillus niger / Q000187:drug effects; D029402:Bignoniaceae; D005670:Fusarium / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D000935:Antifungal Agents; D010936:Plant Extracts