Specific band observed in VCD predicts the anomeric configuration of carbohydrates.
The vibrational circular dichroism (VCD) technique was applied to carbohydrates and produced a glycoside characteristic VCD band, which was named the "glycoside band." It confirmed that this vibration was related to anomeric configurations of carbohydrates in the case of the d-series by means of isotope substitution. This band is defined as a negative, sharp, and intense VCD band at around 1145 cm-1, exhibited by d-sugars with an axial alpha-glycosidic linkage in the 4C1 conformation. As applications of this band, the VCD studies of the maltose-cellobiose mixture and the monitoring of the amyloglucosidase reaction were examined.
Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genomic Science and Technology, Hokkaido University, Kita-ku, Sapporo 001-0021, Japan. kmonde@glyco.sci.hokudai.ac.jp
Kenji
K
Monde
Tohru
T
Taniguchi
Nobuaki
N
Miura
Shin-Ichiro
S
Nishimura
D002236:Carbohydrate Conformation; D002475:Cellobiose / Q000737:chemistry; D002942:Circular Dichroism / Q000379:methods; D004187:Disaccharides / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D008320:Maltose / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods
D004187:Disaccharides; D006027:Glycosides; D002475:Cellobiose; D008320:Maltose; D005947:Glucose