Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.
Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rRNA gene sequencing is a reliable tool for rapid genus-level identification of Brucella spp. and their differentiation from closely related organisms.
Gee
Jay E
JE
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, MS-D11, 1600 Clifton Rd., N.E., Atlanta, GA 30333, USA. JGee1@cdc.gov
De
Barun K
BK
Levett
Paul N
PN
Whitney
Anne M
AM
Novak
Ryan T
RT
Popovic
Tanja
T
D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015203:Reproducibility of Results
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S