Involvement of Leishmania donovani major surface glycoprotein gp63 in promastigote multiplication.
The major surface glycoprotein gp63 of the kinetoplastid protozoal parasite Leishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing gp63 antisense RNA from an episomal vector in L. donovani promastigotes, gp63-deficient transfectants were obtained. Reduction of the gp63 level resulted in increased generation times, altered cell morphology, accumulation of cells in the G2/M phase of the cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth was stimulated, and the number of binucleate cells reduced, by addition to the culture of a bacterially expressed fusion protein containing the fibronectin-like SRYD motif and the zinc-binding (metalloprotease) domain of gp63. These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like properties of gp63 may be important in this process
Indian Institute of Chemical Biology, Kolkata, India.
Sanjeev
S
Pandey
Phuljhuri
P
Chakraborti
Rakhi
R
Sharma
Santu
S
Bandyopadhyay
Dwijen
D
Sarkar
Samit
S
Adhya
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D020868:Gene Silencing; D007893:Leishmania donovani / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D015800:Protozoan Proteins / Q000502:physiology*; D016372:RNA, Antisense; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D015800:Protozoan Proteins; D016372:RNA, Antisense; D011993:Recombinant Fusion Proteins; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania