[Purification and characterization of sanguicin--a bacteriocin produced by Streptococcus sanguis].
OBJECTIVE
Streptococcus sanguis plays an important Role in maintaining the periodontal microecological balance. Previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by S. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (PPB). The aim of this study was to purify sanguicin and investigate its characters.


METHODS
The raw extract of sanguicin was obtained from cells of S. sanguis by ultrasonication, salting out and dialysis. Then the diethyl-aminoethyl-sepharose gradual washing was done in conjunction with glucosan gel filtration for in the purfication of sanguicin. The characters of purified sanguicin were determined and its inhibitiory effect on PPB was measured by agar diffusion test.


RESULTS
The purifed sanguicin was obtained; it was heat labile and proteinaceous; and by SDS-PAGE, it showed a main band with the relative molecular mass of 65 x 10(3). The purifed sanguicin had a strong inhibitory effect on P. gingivalis, P. intermedia and F. nucleatum in vitro.


CONCLUSIONS
Our methods are useful for the purification of sanguicin. Sanguicin has inhibitory effect on PPB obviously and will have a good prospect in periodontal ecological therapy.
Department of Oral Medicine, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Hui
H
Deng
Yi
Y
Ding
Ming-de
MD
Fu
Xiao-rong
XR
Xiao
Juan
J
Liu
Ting
T
Zhou
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D016967:Fusobacterium nucleatum / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D013298:Streptococcus sanguis / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C022273:sanguicin