Crystallization of microsomal triglyceride transfer protein from bovine liver.
The microsomal triglyceride transfer protein (MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7.0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P2(1)2(1)2(1) with refined lattice constants of a = 88.7, b = 100.9 and c = 201.1 A, with one heterodimer per asymmetric unit.
Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543, USA.
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Ohringer
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Jamil
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Chu
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Tabernero
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Einspahr
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Wetterau
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Sack