Chemotaxis of Pseudomonas stutzeri OX1 and Burkholderia cepacia G4 toward chlorinated ethenes.
The chemotactic responses of Pseudomonas putida F1, Burkholderia cepacia G4, and Pseudomonas stutzeri OX1 were investigated toward toluene, trichloroethylene (TCE), tetrachloroethylene (PCE), cis-1,2-dichloroethylene (cis-DCE), trans-1,2-dichloroethylene (trans-DCE), 1,1-dichloroethylene (1,1-DCE), and vinyl chloride (VC). P. stutzeri OX1 and P. putida F1 were chemotactic toward toluene, PCE, TCE, all DCEs, and VC. B. cepacia G4 was chemotactic toward toluene, PCE, TCE, cis-DCE, 1,1-DCE, and VC. Chemotaxis of P. stutzeri OX1 grown on o-xylene vapors was much stronger than when grown on o-cresol vapors toward some chlorinated ethenes. Expression of toluene-o-xylene monooxygenase (ToMO) from touABCDEF appears to be required for positive chemotaxis attraction, and the attraction is stronger with the touR (ToMO regulatory) gene.
Department of Chemical Engineering, University of Connecticut, 191 Auditorium Road, U-3222, Storrs, CT 06269, USA.
Gönül
G
Vardar
Paola
P
Barbieri
Thomas K
TK
Wood
D000222:Adaptation, Physiological; D016956:Burkholderia cepacia / Q000235:genetics / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D008853:Microscopy; D010105:Oxygenases / Q000235:genetics / Q000502:physiology; D016958:Pseudomonas putida / Q000235:genetics / Q000502:physiology; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000502:physiology*
D006843:Hydrocarbons, Chlorinated; D010105:Oxygenases; C114360:toluene 2-xylene monooxygenase