[Studies on the characteristic of interferon-gamma mediating resistance in mice infected with Schistosoma japonicum].
OBJECTIVE
To investigate the molecular characteristic of interferon-gamma mediating protective immunity against schistosomiasis japonica in mice.


METHODS
CD4+ T cells were isolated from spleens of mice infected with Schistosoma japonicum at different time-points. The cDNA microarray technique combined with RT-PCR was used to explore IFN-gamma inducible GTPase family gene expression profile of CD4+ T cell. IGTP, a representative IFN-gamma, inducible GTPase having vital anti-infection activity, was amplified from spleen of BALB/c mice using RT-PCR, then cloned into pGEM(r)-T easy vector for sequencing.


RESULTS
IFN-gamma inducible GTPase family had the similar characteristic over the course of S. japonicum infection. The gene expression of these members were up-regulated or had little change at 3 wk post-infection, then down-modulated from 6 wk to 13 wk post-infection, which was also confirmed by RT-PCR. As for IGTP, two inserts were identified after sequencing. One was 142 bp shorter than another, but the fragment was lost due to low annealing temperature.


CONCLUSIONS
There is a dramatic inhibition of IFN-gamma pathway and IFN-gamma-dependent anti-infective immunity during the infection of S. japonicum.
Ji
Min-jun
MJ
Institute for Molecular Biology of Medicine, Nanjing Medial University, Nanjing 210029, China.
Cai
Xiao-ping
XP
Su
Chuan
C
Wu
Hai-wei
HW
Li
Guang-fu
GF
Wang
Yong
Y
Zhu
Xiang
X
Wang
Xin-jun
XJ
Zhang
Zhao-song
ZS
Wu
Guan-ling
GL
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D007371:Interferon-gamma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation
D007371:Interferon-gamma; D020558:GTP Phosphohydrolases