Effects of contrast media on porcine bone marrow-derived mononuclear cells and calf myoblast viability and secretion of VEGF and MCP-1.
We investigated the effect of contrast media on bone marrow-derived cell viability, growth factor secretion, and myoblast viability. Bone marrow was exposed to contrast media, mononuclear cells were isolated, viability was assessed by Trypan blue exclusion or cultured for 4 weeks, and conditioned medium was assayed for vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1). Skeletal myoblasts viability was assessed after exposing them to contrast media. In separate experiments, bone marrow or bone marrow-derived mononuclear cells were exposed to contrast media, cultured for 40 hr, then assessed for viability. None of the contrast media tested had any effect on bone marrow-derived cell viability. Hypaque or Hexabrix increased myoblasts viability by 8-10%. VEGF and MCP-1 concentrations in the conditioned medium increased in a time-related manner. These findings support the concept that for cell therapy, bone marrow cells or myoblasts may be mixed with contrast media and injected into ischemic myocardium without compromise in viability or function.
Cardiovascular Research Institute, Washington Hospital Center, Washington, District of Columbia.
Richard
R
Baffour
Rajbabu
R
Pakala
David
D
Hellinga
Rufus
R
Seabron
Jana
J
Fournadjiev
Roswitha
R
Wolfram
Petros
P
Okubagzi
Stephen E
SE
Epstein
Ron
R
Waksman
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D003287:Contrast Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D032448:Myoblasts, Skeletal / Q000187:drug effects*; D013552:Swine; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D018932:Chemokine CCL2; D003287:Contrast Media; D042461:Vascular Endothelial Growth Factor A