Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1.
A novel, oil-degrading bacterium (strain T1) was isolated from a hot spring in Hokkaido, Japan. It efficiently degrades different types of fats and oils, including edible oil waste. When grown in a mineral salt medium containing 1% triacylglycerol (as salad oil), hydrolysis products were 1,3- and 1,2-diacylglycerols, monoacylglycerol, and free fatty acid. However, these products were almost completely consumed during cultivation at 30 degrees C for 5 days, indicating that extracellular lipase acts randomly at different sn-positions of acylglycerols and that strain T1 has a high capacity to utilize free fatty acids. Secreted lipase activity was induced by salad oil and oleic acid. This strain was a Gram-negative straight rod shaped, aerobic, with a polar flagellum, capable of growing in temperature ranges between 15 degrees C and 55 degrees C. The 16S rRNA gene sequence analysis and DNA-DNA hybridization revealed it as a new strain of Pseudomonas aeruginosa. The type strain was T1.
Hasanuzzaman
Mohammad
M
Graduate School of Environmental Earth Sciencee, Hokkaido University, Sapporo 064-0804, Japan.
Umadhay-Briones
Kathryn M
KM
Zsiros
Szilvia M
SM
Morita
Naoki
N
Nodasaka
Yoshinobu
Y
Yumoto
Isao
I
Okuyama
Hidetoshi
H
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004075:Diglycerides / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005407:Flagella / Q000648:ultrastructure; D020459:Genes, rRNA; D005989:Glycerides / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology; D007564:Japan; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D009821:Oils / Q000378:metabolism*; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014280:Triglycerides / Q000378:metabolism
C011439:1,2-diacylglycerol; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004075:Diglycerides; D005227:Fatty Acids; D005989:Glycerides; D009821:Oils; D012336:RNA, Ribosomal, 16S; D014280:Triglycerides; D008049:Lipase