Toward a robust model of packing and scale-up for chromatographic beds. 2. Flow packing.
We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical compression (consolidation) test is used, the model captures the primary phenomena of the scale-up process. However, moduli determined from flow-packing experiments improve the accuracy of the predictions and show that the apparent rigidity of chromatographic resins is lower for flow packing than for mechanical compression. Using a modulus from flow-packing experiments provided quantitative scale-up predictions of flow packing carried out in columns with diameters between 200 and 450 mm at different locations and by different operators.
Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22904-4741, USA.
Ronald N
RN
Keener
James E
JE
Maneval
Erik J
EJ
Fernandez
D008962:Models, Theoretical; D010316:Particle Size; D010539:Permeability; D010865:Pilot Projects; D012680:Sensitivity and Specificity