Metabolism of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine by Clostridium bifermentans strain HAW-1 and several other H2-producing fermentative anaerobic bacteria.
Several H2-producing fermentative anaerobic bacteria including Clostridium, Klebsiella and Fusobacteria degraded octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) (36 microM) to formaldehyde (HCHO) and nitrous oxide (N2O) with rates ranging from 5 to 190 nmol h(-1)g [dry weight] of cells(-1). Among these strains, C. bifermentans strain HAW-1 grew and transformed HMX rapidly with the detection of the two key intermediates the mononitroso product and methylenedinitramine. Its cellular extract alone did not seem to degrade HMX appreciably, but degraded much faster in the presence of H2, NADH or NADPH. The disappearance of HMX was concurrent with the release of nitrite without the formation of the nitroso derivative(s). Results suggest that two types of enzymes were involved in HMX metabolism: one for denitration and the second for reduction to the nitroso derivative(s).
Zhao
Jian-Shen
JS
Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Que., Canada H4P 2R2.
Paquet
Louise
L
Halasz
Annamaria
A
Manno
Dominic
D
Hawari
Jalal
J
D000693:Anaerobiosis; D001392:Azocines / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003013:Clostridium / Q000378:metabolism*; D005285:Fermentation; D005557:Formaldehyde / Q000378:metabolism; D045182:Fusobacteria / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D007709:Klebsiella / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D012989:Soil Pollutants / Q000378:metabolism
D001392:Azocines; D006573:Heterocyclic Compounds, 1-Ring; D009573:Nitrites; D012989:Soil Pollutants; D009243:NAD; D005557:Formaldehyde; D009249:NADP; D006859:Hydrogen; D009609:Nitrous Oxide; C007950:octogen