Characterization of molecular transport in poly(dimethylsiloxane) microchannels for electrophoresis fabricated with synchrotron radiation-lithography and UV-photolithography.
In the present paper, a study was undertaken of molecular transport in ploy(dimethylsiloxane) microchannels that were fabricated by ultraviolet (UV)-photolithography and synchrotron radiation (SR)-lithography characterized and compared for microchip capillary electrophoresis by evaluating in-channel molecular dispersion. A fluorescent tag, sulforhodamine B was used as the probing molecule. It was found that microchannels made by SR-lithography fabrication were superior to those made by UV-photolithography fabrication in terms of molecular transport performance. A deep insight into surface conditions characterized by scanning electron microscopy suggested it was related to the difference in surface roughness. Chromatographic retention in electropherograms further supported such a conclusion, which depended on the phase ratio of the channel surface. The results revealed for PDMS microchannels in this work were in good agreement with the phenomenon found for glass microchannels in the literature.
The Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
Bi-Feng
BF
Liu
Motoaki
M
Ozaki
Hideaki
H
Hisamoto
Yuichi
Y
Utsumi
Tadashi
T
Hattori
Shigeru
S
Terabe
D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000528:radiation effects; D019075:Electrophoresis, Capillary / Q000379:methods*; D004867:Equipment Design / Q000295:instrumentation; D044085:Microfluidics / Q000295:instrumentation; D009038:Motion; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects; D012235:Rhodamines / Q000737:chemistry* / Q000528:radiation effects; D017356:Synchrotrons; D014466:Ultraviolet Rays
D004129:Dimethylpolysiloxanes; D011108:Polymers; D012235:Rhodamines; C022027:lissamine rhodamine B