Determination of thiabendazole in orange juice and rind by liquid chromatography with fluorescence detection and confirmation by gas chromatography/mass spectrometry after extraction by matrix solid-phase dispersion.
A method was developed for the determination of thiabendazole (TBZ) in orange juice and rind based on matrix solid-phase dispersion (MSPD). TBZ was extracted with ethyl acetate and the extract was subsequently cleaned up on a solid-phase extraction column. Fungicide residues were determined by liquid chromatography with fluorescence detection. Recoveries through the method ranged from 87 to 97% with relative standard deviations < or = 11%. The detection and quantitation limits were 0.15 and 0.50 microg/kg, respectively. The confirmation of TBZ residues in positive samples was performed by solid-phase microextraction followed by gas chromatography with mass spectrometric detection using selected ion monitoring. The developed method was applied to determine TBZ levels in commercial orange juices and in juice and rind of fresh oranges. The influence of storage and washing of fruits on TBZ residues was also studied.
INIA, Departamento de Medio Ambiente, INIA, Apdo. 8111, 28080 Madrid, Spain.
Beatriz
B
Albero
Consuelo
C
Sánchez-Brunete
José L
JL
Tadeo
D001628:Beverages / Q000032:analysis*; D002853:Chromatography, Liquid; D002957:Citrus / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013827:Thiabendazole / Q000032:analysis*
D005659:Fungicides, Industrial; D007202:Indicators and Reagents; D010573:Pesticide Residues; D012997:Solvents; D013827:Thiabendazole