Surfactant protein D binding to terminal alpha1-3-linked fucose residues and to Schistosoma mansoni.
Pulmonary surfactant protein (SP)-D is an important component of the innate immune system of the lung, which is thought to function by binding to specific carbohydrates on the surface of viruses and unicellular pathogens. SP-D has been shown to have a relatively high affinity for the monosaccharides mannose, glucose, and fucose. However, there is limited information on SP-D binding to complex carbohydrate structures, and binding of SP-D to fucose in the context of an oligosaccharide has not yet been investigated. In this study, we used surface plasmon resonance spectroscopy to examine the potential of SP-D to bind to various synthetic fucosylated oligosaccharides, and identified Fucalpha1-3GalNAc and Fucalpha1-3GlcNAc elements as strong ligands. These types of fucosylated glycoconjugates are presented at the surface of Schistosoma mansoni, a parasitic worm that, during development, transiently resides in the lung. In line with the findings by surface plasmon resonance, we found that SP-D can bind to larval stages of S. mansoni, demonstrating for the first time that SP-D interacts with multicellular lung pathogens.
van de Wetering
J Koenraad
JK
Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. j.j.vanhellemond@vet.uu.nl
van Remoortere
Alexandra
A
Vaandrager
Arie B
AB
Batenburg
Joseph J
JJ
van Golde
Lambert M G
LM
Hokke
Cornelis H
CH
van Hellemond
Jaap J
JJ
D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008024:Ligands; D008168:Lung / Q000166:cytology; D018613:Microscopy, Confocal; D008956:Models, Chemical; D011485:Protein Binding; D037663:Pulmonary Surfactant-Associated Protein D / Q000737:chemistry* / Q000378:metabolism; D012550:Schistosoma mansoni / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013997:Time Factors
D002241:Carbohydrates; D006023:Glycoproteins; D008024:Ligands; D037663:Pulmonary Surfactant-Associated Protein D; D003208:Concanavalin A; D005643:Fucose