Bactericidal activity of glycinecin A, a bacteriocin derived from Xanthomonas campestris pv. glycines, on phytopathogenic Xanthomonas campestris pv. vesicatoria cells.
The ability of glycinecin A, a bacteriocin derived from Xanthomonas campestris pv. glycines 8ra, to kill closely related bacteria has been demonstrated previously by our group. In the present study, we aimed at determining the glycinecin A-induced cause of death. Treatment with glycinecin A caused slow dissipation of membrane potential and rapid depletion of the pH gradient. Glycinecin A treatment also induced leakage of potassium ions from X. campestris pv. vesicatoria YK93-4 cells and killed sensitive bacterial cells in a dose-dependent manner. Sensitive cells were killed within 2 h of incubation, most likely due to the potassium ion efflux caused by glycinecin A. These results suggest that the bactericidal mechanism of action of glycinecin A is correlated with the permeability of membranes to hydroxyl and potassium ions, leading to the lethal activity of the bacteriocin on the target bacteria.
Pham
Huy Thang
HT
Department of Biochemistry, Cheju National University Medical College, Ara 1, Jeju 690-756, Korea.
Riu
Key Zoung
KZ
Jang
Kong Man
KM
Cho
Somi K
SK
Cho
Moonjae
M
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D010935:Plant Diseases / Q000382:microbiology*; D011188:Potassium / Q000378:metabolism; D018892:Proton-Motive Force / Q000187:drug effects; D016959:Xanthomonas campestris / Q000145:classification / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C439290:glycinecin A; D011188:Potassium