Immunohistochemical diagnosis of mouse hepatitis virus and mycoplasma pulmonis infection with murine antiserum.
This study established a modified alkaline phosphatase-labelled avidin-biotin-complex (ABC-AP) method for diagnosis of mouse hepatitis virus (MHV) and Mycoplasma pulmonis infection from formalin-fixed, paraffin wax-embedded sections, murine antibody-positive serum being used as the primary reagent. With this method, MHV antigen in cAnNCrj.Cg-Foxn1(nu)/Foxn1(nu) mice and M. pulmonis antigen in Wistar rats were immunolabelled in tissue sections. MHV antigen was clearly detected in samples of liver, stomach, caecal and colonic mucosa, and spleen. M. pulmonis antigen was demonstrated on the luminal surface of bronchiolar epithelial cells. This method may prove useful in diagnosis when commercial antisera are unavailable or when immunosuppression prevents serological diagnosis.
Liang
C T
CT
National Laboratory Animal Center, National Applied Research Laboratories, Nan-Kang, Taipei 115, Taiwan, ROC.
Wu
S C
SC
Huang
Y T
YT
Lin
Y C
YC
Chang
W J
WJ
Chou
J Y
JY
Liang
S C
SC
Liu
C H
CH
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005260:Female; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D006517:Murine hepatitis virus / Q000276:immunology; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D045803:Mycoplasma pulmonis / Q000276:immunology; D051381:Rats
D000942:Antigens, Bacterial; D000956:Antigens, Viral; D007106:Immune Sera