Characterization of a myostatin-like gene from the bay scallop, Argopecten irradians.
A complete cDNA was cloned from the bay scallop (Argopecten irradians) that codes for a 382-amino-acid myostatin-like protein (sMSTN). The sMSTN sequence is most similar to mammalian myostatin (MSTN), containing a conserved proteolytic cleavage site (RXXR) and conserved cysteine residues in the C-terminus. Based on quantitative RT-PCR, the sMSTN gene is predominantly expressed in the adductor muscle, with limited expression in other tissues. Using the sMSTN sequence, a Ciona MSTN-like gene was also identified from the Ciona intestinalis genome. These findings indicate that the MSTN gene has been conserved throughout evolution and suggests that MSTN could play a major role in muscle growth and development in invertebrates, as it does in mammals.
Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
Hyun-Woo
HW
Kim
Donald L
DL
Mykles
Frederick W
FW
Goetz
Steven B
SB
Roberts
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D055435:Myostatin; D012313:RNA / Q000032:analysis; D016415:Sequence Alignment; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C525911:MSTN protein, human; D055435:Myostatin; D016212:Transforming Growth Factor beta; D012313:RNA