The molecular basis of adaptive evolution of squirrelfish rhodopsins.
The wavelengths of maximal absorption (lambdamax) of the rhodopsins of nine squirrelfishes (N. sammara, N. argenteus, S. punctatissimum, S. microstoma, S. diadema, S. xantherythrum, S. spiniferum, N. aurolineatus, and S. tiere) and two soldierfishes (M. violacea and M. berndti) vary between 481 and 502 nm. Phylogenetic and mutagenesis analyses suggest that the common ancestor of these pigments had a lambdamax value of approximately 493 nm, and the contemporary lambdamax values were generated mostly by amino acid replacements E122M, F261Y, and A292S. The probability of observing all these amino acid replacements at specific branches of the phylogenetic tree is only 2.5 x 10(-9); it is highly unlikely that these changes have occurred by neutral evolution. Because of a close association between the lambdamax values of these pigments and the wavelengths of light available to the corresponding species, the excess number of amino acid changes at specific branches in the phylogenetic tree strongly suggests that the rhodopsins have undergone adaptive changes at various stages of the holocentrid evolution.
Yokoyama
Shozo
S
Department of Biology, Emory University, Atlanta, Georgia, USA. syokoya@emory.edu
Takenaka
Naomi
N
D000818:Animals; D005075:Biological Evolution; D002417:Cattle; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D008027:Light; D016296:Mutagenesis; D010802:Phylogeny; D017354:Point Mutation; D012168:Retinal Pigments / Q000235:genetics; D012243:Rhodopsin / Q000235:genetics*; D014867:Water
D012168:Retinal Pigments; D014867:Water; D012243:Rhodopsin