Myocardial contrast echocardiography in assessing microcirculation in baboons with chagas disease.
OBJECTIVE
Microvascular abnormalities have been postulated in the pathogenesis of chagasic cardiomyopathy. The objective of this study was to evaluate the relationship between coronary microcirculation and systolic function impairment in baboons with Chagas disease using myocardial contrast echocardiography (MCE).


METHODS
Seventeen seropositive (5 males, 12 females; mean age 20 years) and 13 age- and gender-matched seronegative baboons underwent MCE using intravenous octafluoropropane human albumin microspheres. Color-coding was used to enhance tissue contrast in assessing regional myocardium uniformity and texture. Dipyridamole (0.54 mg/kg) was given to a subset of 4 animals to challenge coronary flow reserve. Systolic indices included left ventricular fractional shortening, velocity of circumferential fiber shortening, and left and right ventricular ejection fractions.


RESULTS
Four of the 17 (24%) seropositive primates had decreased fractional shortening (25 +/- 8% vs. 40 +/-5%, p <.005), velocity of circumferential fiber shortening (1.05 +/- 0.36 circ/s vs. 1.84 +/- 0.23 circ/s, p <.0001), and reduced right ventricular ejection fraction (44 +/- 9% vs. 54 +/- 4%, p <.05) compared to other seropositive animals. Seropositive and seronegative groups showed no significant differences on the coronary microcirculation pattern as evaluated by MCE, including the 4 baboons with systolic function impairment. Moreover, coronary flow vasoreactivity resulted in a significant increase in myocardial flow as detected by color-coding masking.


CONCLUSIONS
Chagasic heart disease is present in 24% of seropositive baboons spontaneously infected with Trypanosoma cruzi. MCE reveals a discrepancy between coronary microcirculation at rest and alterations in myocardial contractility, suggesting preservation of the microvascular integrity in this unique animal model.
The University of Texas Health Science Center at San Antonio, Division of Cardiology, San Antonio, Texas 78229-3900, USA. Zabalgoitia@uthscsa.edu
Miguel
M
Zabalgoitia
Jaime
J
Ventura
Jose L
JL
Lozano
Lori
L
Anderson
K D
KD
Carey
G B
GB
Hubbard
Jeff T
JT
Williams
John L
JL
Vandeberg
D000418:Albumins; D000818:Animals; D014355:Chagas Disease / Q000209:etiology / Q000503:physiopathology*; D003326:Coronary Circulation; D004452:Echocardiography / Q000379:methods*; D005260:Female; D005466:Fluorocarbons; D006334:Heart Function Tests; D015994:Incidence; D008297:Male; D008833:Microcirculation / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology; D010215:Papio; D014349:Trypanosoma cruzi
D000418:Albumins; C099458:FS 069; D005466:Fluorocarbons