In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes.
Chitosan-silicate hybrids were synthesized using gamma-glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.
Biomaterial Laboratory, Faculty of Engineering, Okayama University, 3-1-1 Tsushima-naka Okayama-shi 700-8530, Japan.
Yuki
Y
Shirosaki
Kanji
K
Tsuru
Satoshi
S
Hayakawa
Akiyoshi
A
Osaka
Maria Ascensão
MA
Lopes
José Domingos
JD
Santos
Maria Helena
MH
Fernandes
D000469:Alkaline Phosphatase / Q000032:analysis; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001859:Bone Neoplasms / Q000473:pathology; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009363:Neoplasm Proteins / Q000032:analysis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012516:Osteosarcoma / Q000473:pathology; D012821:Silanes / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D017155:Wettability
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D009363:Neoplasm Proteins; D012821:Silanes; C039462:gamma-glycidoxypropyltrimethoxysilane; D048271:Chitosan; D000469:Alkaline Phosphatase; D002118:Calcium