A synthetic peptide from a heptad repeat region of herpesvirus glycoprotein B inhibits virus replication.
Glycoprotein B (gB) is the most conserved glycoprotein of herpesviruses and plays important roles in virus infectivity. Two intervening heptad repeat (HR) sequences were found in the C-terminal half of all herpesvirus gBs analysed. A synthetic peptide derived from the HR region (aa 477-510) of bovine herpesvirus type 1 (BoHV-1) gB was studied for its ability to inhibit virus replication. The peptide interfered with cell-to-cell spread and consistently inhibited replication of BoHV-1, with a 50 % effective concentration value (EC(50)) of 5 microM. Inhibition of replication was obtained not only with herpesviruses including pseudorabies virus and herpes simplex virus type 1 but also partly with Newcastle disease virus. Possible mechanisms of membrane fusion inhibition by the peptide are discussed.
Okazaki
Katsunori
K
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Kida
Hiroshi
H
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D020449:Repetitive Sequences, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000494:pharmacology*; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects*
D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins