[Impact of traditional Chinese medicine WPY on ICAM-1 expression and MPO activity in pancreas and lungs of rats with acute necrotizing pancreatitis].
OBJECTIVE
To explore the character of the ICAM-1 induced change of polymorphonuclear neutrophil (PMN) in the pancreas and lung of rats with acute necrotizing pancreatitis (ANP) and to evaluate the effects of Chinese medicine WPY on ANP.


METHODS
ANP model was induced by retrograde injection of 3.5% sodium taurocholic acid into the biliopancreatic duct of Wistar rats. Expression of ICAM-1 at different time points in pancreas and lung was assessed by immunohistochemistry. The quantity of polymorphonuclear neutrophil (PMN) in tissues was measured by the level of myeloperoxidase activity (MPO). In addition, the indices above were also observed after the administration of WPY.


RESULTS
In the ANP group, ICAM-1 expression and MPO activity were evidently up-regulated at 3 h in pancreas and at 6 h in lung, and both reached their highest level at 12 h. In the WPY treated group, ICAM-1 expression and MPO activity in pancreas and lung were significantly decreased at 12 h.


CONCLUSIONS
The numerous PMNs infiltration mediated by ICAM-1 plays an important role in the damage to pancreas and lung caused by ANP. The traditional Chinese medicine WPY can attenuate ICAM-1 expression and MPO activity in pancreas and lung during ANP.
Wang
Jiao
J
Department of Traditional Chinese Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Jiang
Jun-ming
JM
Cheng
Wei-bo
WB
Zhang
Zhao-da
ZD
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000378:metabolism; D019283:Pancreatitis, Acute Necrotizing / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012250:Rheum / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase