Protective effects of barley and its hydrolysates on gastric stress ulcer in rats.
This research intends to clarify the protective effect of barley and its hydrolysates with respect to a water immersion stress-induced ulcer in the rat model. The beta-(1-->3)-glucan content of barley, and specifically beta-(1-->4),(1-->3)-glucan content was determined and then gastric stress ulcerogenesis induced by water immersion was conducted using five-week-old male Sprague-Dawley rats (7 rats in one group). The barley diet group was fed 10% barley flour that was substituted with sucrose in the control diet. For the 3 groups fed on soluble dietary fiber (SDF), the diets were supplemented with 0.46 g of SDF, equivalent to 100 g of the control diet; 0.46 g of SDF is equivalent to 10 g of barley flour. The rats were housed in a stress-cage and immersed in a water bath (23 degrees C) up to their necks for 21 h. The content of SDF and beta-(1-->3)-glucan content in barley flour were 4.6% and 3.4%, respectively. Although strongly anti-ulcer activities were observed in the barley (10%), SDF isolated and beta-(1-->3)-glucan fraction (Hydrolysate I) prepared from barley flour after treatment with lichenase, in other words, beta-(1-->4),(1-->3)-glucan itself, its hydrolysate (Hydrolysate II) with beta-(1-->3)-glucosidase did not display any anti-ulcer activity. This finding suggests that the beta-(1-->3)-glucosyl-linkage on beta-(1-->3)-glucan is an important part of the active principle for anti-ulcerogenesis.
Department of Nutrition, College of Nutrition, Koshien University, Momijigaoka, Takarazuka, Japan. yoshizawa@koshien.ac.jp
Minako
M
Yoshizawa
Komaki
K
Yokoyama
Yoshihisa
Y
Nakano
Hisao
H
Nakamura
D000818:Animals; D004043:Dietary Fiber / Q000627:therapeutic use; D004195:Disease Models, Animal; D005504:Food Analysis; D005936:Glucans / Q000302:isolation & purification / Q000627:therapeutic use*; D001467:Hordeum / Q000737:chemistry; D007101:Immersion / Q000009:adverse effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013276:Stomach Ulcer / Q000178:diet therapy / Q000209:etiology / Q000517:prevention & control*; D013312:Stress, Physiological / Q000150:complications*; D047071:beta-Glucans
D004043:Dietary Fiber; D005936:Glucans; D047071:beta-Glucans; C067858:beta-glucan, (1-3)(1-4)-