Base-discriminating fluorescent (BDF) nucleoside: distinction of thymine by fluorescence quenching.
A novel fluorescence BDF probe containing pyrene-labeled 7-deaza-2[prime or minute]-deoxyadenosine has been developed for the detection of thymine base on a target DNA.
Saito
Yoshio
Y
NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan. saito@mech.ce.nihon-u.ac.jp
Miyauchi
Yohei
Y
Okamoto
Akimitsu
A
Saito
Isao
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D003839:Deoxyadenosines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008969:Molecular Sequence Data; D009705:Nucleosides / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013941:Thymine / Q000032:analysis* / Q000737:chemistry
D003839:Deoxyadenosines; D005456:Fluorescent Dyes; D009705:Nucleosides; D011721:Pyrenes; D004247:DNA; D013941:Thymine