Signal transducer and activator of transcription 3 (Stat3) expression and activation in rat uterus during early pregnancy.
Signal transducer and activator of transcription 3 (Stat3), a member of the Stat family, is specifically activated during mouse embryo implantation. The aim of this study was to investigate the expression, activation and regulation of Stat3 in rat uterus during early pregnancy, pseudopregnancy, delayed implantation and artificial decidualization. Stat3 mRNA was highly expressed in the luminal epithelium on day 5 and in the luminal epithelium and underlying stromal cells at implantation sites on day 6 of pregnancy. There was a strong level of Stat3 protein expression and phosphorylation in the stromal cells near the lumen and in the luminal epithelium on day 5 of pregnancy, which was similar to day 5 of pseudopregnancy. In the afternoon of day 6, the strong level of Stat3 phosphorylation was detected only in the luminal epithelium. Stat3 was highly expressed and activated in the decidual cells from days 7 to 9 of pregnancy and under artificial decidualization in the present study. Our results suggest that the strong level of Stat3 activation in the luminal epithelium and underlying stromal cells during the pre-implantation period may be important for establishing uterine receptivity as in mice, and the high level of Stat3 expression and activation in decidual cells may play a role during decidualization.
Teng
Chun-Bo
CB
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China and Heilongjiang Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China.
Diao
Hong-Lu
HL
Ma
Hong
H
Cong
Jing
J
Yu
Hao
H
Ma
Xing-Hong
XH
Xu
Li-Bin
LB
Yang
Zeng-Ming
ZM
D000818:Animals; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D003656:Decidua / Q000502:physiology; D010064:Embryo Implantation; D010065:Embryo Implantation, Delayed / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D010766:Phosphorylation; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics*; D014599:Uterus / Q000737:chemistry* / Q000378:metabolism
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators