[Cytoplasmic male sterility-associated structural variation of the mitochondrial genome regions containing rps3 and orf215 in sugar beet Beta vulgaris L].
Several 5'-degenerate primers were selected by computer analysis and used for mtDNA typing in sugar beet cultivars with cytoplasms of the S (typical for cytoplasmic male sterility) or N (normal) type. A number of N- or S-specific markers were found to correspond to transcribed mitochondrial genes. One was from the orf215 region of the N-type mtDNA. A physical map of the corresponding region was constructed for the S-type mtDNA, and a substantial difference observed for the two genome types. One N-specific marker proved to contain a rearranged rps3 region and a truncated atp9 copy. With the known nucleotide sequence of this marker, three-primer PCR was designed and showed that both variants of the rps3 region simultaneously take place in the mtDNA pool, the new one occurring in a substochiometric proportion.
Ivanov
M K
MK
Revenko
A S
AS
Dymshits
G M
GM
D001483:Base Sequence; D027461:Beta vulgaris / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D018745:Genome, Plant; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D015183:Restriction Mapping
D017931:DNA Primers; D004272:DNA, Mitochondrial; D010940:Plant Proteins