Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens.
The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.
Cazacu
Andreea C
AC
Department of Pediatrics, Baylor College of Meidcine, Houston, TX 77030, USA.
Demmler
Gail J
GJ
Neuman
Mark A
MA
Forbes
Betty A
BA
Chung
Sooyoung
S
Greer
Jewel
J
Alvarez
Ana E
AE
Williams
Robin
R
Bartholoma
Nadine Y
NY
D002845:Chromatography / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009980:Influenza A virus / Q000145:classification / Q000302:isolation & purification*; D009981:Influenza B virus / Q000145:classification / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D007753:Laboratories / Q000592:standards; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012137:Respiratory System / Q000821:virology*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic