Correlating function and gene expression of individual basal ganglia neurons.
Functional studies at the level of individual neurons have greatly contributed to our current understanding of basal ganglia function and dysfunction. However, identification of the expressed genes responsible for these distinct neuronal phenotypes is less advanced. Qualitative and quantitative single-cell gene-expression profiling, combined with electrophysiological analysis, allows phenotype-genotype correlations to be made for individual neurons. In this review, progress on gene-expression profiling of individual, functionally characterized basal ganglia neurons is discussed, focusing on ion channels and receptors. In addition, methodological issues are discussed and emerging novel techniques are introduced that will enable a genome-wide comparison of function and gene expression for individual neurons.
Liss
Birgit
B
Molecular Neurobiology, Institute for Physiology, Philipps-University Marburg, Deutschhausstrasse 2, 35033 Marburg, Germany.
Roeper
Jochen
J
D000818:Animals; D001479:Basal Ganglia / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000032:analysis; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D011954:Receptors, Dopamine / Q000235:genetics / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007473:Ion Channels; D012333:RNA, Messenger; D018091:Receptors, AMPA; D011956:Receptors, Cell Surface; D011954:Receptors, Dopamine; D011978:Receptors, Nicotinic