Chromatin immunoprecipitation assays.
Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain. Here, we show the step-by-step methods currently used in our lab to immunoprecipitate the formaldehyde crosslinked chromatin and further analyze the immuprecipitated DNA by semiquantitative PCR.
Department of Environmental Medical, New York University School of Medicine, Tuxedo, NY, USA.
Yan
Y
Yan
Haobin
H
Chen
Max
M
Costa
D002843:Chromatin / Q000737:chemistry* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011233:Precipitin Tests / Q000379:methods*; D012995:Solubility; D013010:Sonication
D002843:Chromatin