Crystallization and preliminary X-ray analysis of a hydantoinase from Arthrobacter aurescens DSM 3745.
L-Hydantoinase from Arthrobacter aurescens DSM 3745 has been purified to homogeneity and crystallized from polyethylene glycol solutions in a form suitable for X-ray diffraction analysis. The crystals have been grown by the sitting-drop variant of the vapour-diffusion method. X-ray diffraction studies show that the crystals belong to the monoclinic space group P2(1) with a = 111.2, b = 74.4, c = 146.5 A and beta = 106.7 degrees. Its asymmetric unit contains four monomers related by 222 symmetry. The crystals diffract to at least 2.6 A.
May
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Universität Stuttgart, Institut für Bioverfahrenstechnik, Germany.
Siemann
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Syldatk
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Niefind
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K
Schomburg
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