[Investigation of the expression of calcitonin receptor mRNA in human osteoclasts on deciduous teeth].
OBJECTIVE
To investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.


METHODS
After fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.


RESULTS
There were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.


CONCLUSIONS
On the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptor mRNA, so it is feasible to use this kind of osteoclast to test the effect of external factors on the expression of CTR mRNA.
Key. Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Ming
M
Chen
Xing
X
Liang
Xiang-jun
XJ
Bao
Hang
H
Wang
Hui-qiang
HQ
Sun
Shao-heng
SH
Lu
D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D010010:Osteoclasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D018003:Receptors, Calcitonin / Q000096:biosynthesis* / Q000235:genetics; D014094:Tooth, Deciduous / Q000166:cytology / Q000378:metabolism*
D012333:RNA, Messenger; D018003:Receptors, Calcitonin