CMY-13, a novel inducible cephalosporinase encoded by an Escherichia coli plasmid.
An IncN plasmid (p541) from Escherichia coli carried a Citrobacter freundii-derived sequence of 4,252 bp which included an ampC-ampR region and was bound by two directly repeated IS26 elements. ampC encoded a novel cephalosporinase (CMY-13) with activity similar to that of CMY-2. AmpR was likely functional as indicated in induction experiments.
Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece. miriagou@mail.pasteur.gr
V
V
Miriagou
L S
LS
Tzouvelekis
L
L
Villa
E
E
Lebessi
A C
AC
Vatopoulos
A
A
Carattoli
E
E
Tzelepi
D002510:Cephalosporinase / Q000096:biosynthesis / Q000235:genetics*; D016971:Citrobacter freundii / Q000201:enzymology / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D065093:beta-Lactamase Inhibitors
D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D065093:beta-Lactamase Inhibitors; C490912:CMY-13 cephalosporinase, E coli; D002510:Cephalosporinase