Crystallization and preliminary X-ray analysis of a histidine kinase domain of the anaerobic sensor protein ArcB from Escherichia coli.
Crystals of a novel histidine protein kinase domain of the anaerobic sensor protein ArcB from Escherichia coli have been obtained by a hanging-drop vapor-diffusion method with micro- and macroseeding techniques. Preliminary X-ray crystallographic analysis revealed that they belong to space group P2(1)2(1)2(1) with dimensions a = 30.56, b = 34.93 and c = 110.78 A, having one molecule in the crystallographic asymmetric unit. The crystals diffract to at least 2.0 A resolution.
Department of Molecular Biology, Nara Institute of Science and Technology, Ikoma, Japan.
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Kato
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Ishige
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Mizuno
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Shimizu
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Hakoshima