Heparan sulfate-independent infection attenuates high-neurovirulence GDVII virus-induced encephalitis.
The high-neurovirulence Theiler's murine encephalomyelitis virus (TMEV) strain GDVII uses heparan sulfate (HS) as a coreceptor to enter target cells. We report here that GDVII virus adapted to growth in HS-deficient cells exhibited two amino acid substitutions (R3126L and N1051S) in the capsid and no longer used HS as a coreceptor. Infectious-virus yields in CHO cells were 25-fold higher for the adapted virus than for the parental GDVII virus, and the neurovirulence of the adapted virus in intracerebrally inoculated mice was substantially attenuated. The adapted virus showed altered cell tropism in the central nervous systems of mice, shifting from cerebral and brainstem neurons to spinal cord anterior horn cells; thus, severe poliomyelitis, but not acute encephalitis, was observed in infected mice. These data indicate that the use of HS as a coreceptor by GDVII virus facilitates cell entry and plays an important role in cell tropism and neurovirulence in vivo.
Department of Neurology, Evanston Hospital, 2650 Ridge Ave., Evanston, IL 60201, USA.
Honey V
HV
Reddi
A S Manoj
AS
Kumar
Aisha Y
AY
Kung
Patricia D
PD
Kallio
Brian P
BP
Schlitt
Howard L
HL
Lipton
D019943:Amino Acid Substitution; D000818:Animals; D001921:Brain / Q000166:cytology / Q000821:virology; D016466:CHO Cells; D018188:Cardiovirus Infections / Q000503:physiopathology* / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D018792:Encephalitis, Viral / Q000503:physiopathology* / Q000821:virology; D006497:Heparan Sulfate / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000821:virology; D011051:Poliomyelitis / Q000503:physiopathology* / Q000821:virology; D013116:Spinal Cord / Q000166:cytology / Q000821:virology; D017945:Theilovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
D006497:Heparan Sulfate