Identification of (+)-phyllocladene, (--)-sandaracopimaradiene, and (+)-kaurene as new fungal metabolites from fusicoccin-producing Phomopsis amygdali F6.
A chemical analysis of the diterpene hydrocarbons produced by fusicoccin-producing fungus Phomopsis amygdali F6 identified five phyllocladene-related tri- and tetracyclic diterpene hydrocarbons. The presence of (+)-phyllocladene, (--)-sandaracopimaradiene, (+)-isopimara-8,15-diene, and (+)-pimara-8(14),15-diene in the fungus was demonstrated by GC-MS, 1H-NMR, and [alpha]D measurements. (+)-Kaurene was also identified by GC-MS and chiral capillary GC. The possible biosynthetic relationship of these metabolites is discussed.
Course of the Science of Bioresources, The United Graduate School of Agricultural Sciences, Iwate University (Yamagata University), Wakaba-cho, Tsuruoka 997-8555, Japan.
Hiromichi
H
Kenmoku
Mio
M
Tanaka
Koichi
K
Ogiyama
Nobuo
N
Kato
Takeshi
T
Sassa
D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation
D004224:Diterpenes; D006027:Glycosides; C007808:fusicoccin