Leech filamin and Tractin: markers for muscle development and nerve formation.
The Lan3-14 and Laz10-1 monoclonal antibodies recognize a 400 kDa antigen that is specifically expressed by all muscle cells in leech. We show that the antigen recognized by both antibodies is a member of the filamin family of actin binding proteins. Leech filamin has two calponin homology domains and 35 filamin/ABP-repeat domains. In addition, we used the Laz10-1 antibody to characterize the development of the segmentally iterated dorsoventral flattener muscles. We demonstrate that the dorsoventral flattener muscle develops as three discrete bundles of myofibers and that CNS axons pioneering the DP nerve extend only along the middle bundle. Interestingly, the middle dorsoventral muscle anlage is associated with only non-neuronal expression of the L1-family cell adhesion molecule Tractin. This expression is transient and occurs at the precise developmental stages when DP nerve formation takes place. Based on these findings we propose that the middle dorsoventral muscle anlagen provides a substrate for early axonal outgrowth and nerve formation and that this function may be associated with differential expression of distinct cell adhesion molecules.
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA.
Deepa V
DV
Venkitaramani
Dong
D
Wang
Yun
Y
Ji
Ying-Zhi
YZ
Xu
Liliana
L
Ponguta
Katie
K
Bock
Birgit
B
Zipser
John
J
Jellies
Kristen M
KM
Johansen
Jørgen
J
Johansen
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular / Q000379:methods; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D064448:Filamins; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007865:Leeches; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development / Q000502:physiology; D009132:Muscles / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017421:Sequence Analysis / Q000379:methods; D014404:Tubulin / Q000378:metabolism
D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D012333:RNA, Messenger; D014404:Tubulin; C107284:tractin protein, Hirudo medicinalis