Micro patterning of active proteins with perforated PDMS sheets (PDMS sieve).
We propose a novel technique for patterning active proteins on a glass substrate using a perforated polydimethylsiloxane (PDMS) sheet-sieve. The sieve, which has tapering holes, is fabricated by spin-coating PDMS on a pyramidal-shaped mold. By means of this sieve, FITC (fluorescent isothiocyanate, bovine)-albumin was successfully spotted in a 5 x 5 microm(2) area in an array. The patterned spots were perfectly isolated, which eliminates the problem of non-specific binding of proteins to undesired areas. To show that proteins maintained their activity after the patterning, we used F(1)-ATPase biomolecular motors; their activity can easily be verified by observing their rotary motion after patterning. Selective patterning with three kinds of fluorescent micro beads indicated the possibility of patterning of different proteins on the same substrate by using the sieve.
Atsuta
Kyoko
K
Center for International Research on Micro Mechatronics (CIRMM), Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. atsuta@iis.u-tokyo.ac.jp
Noji
Hiroyuki
H
Takeuchi
Shoji
S
D000251:Adenosine Triphosphatases / Q000737:chemistry; D000418:Albumins / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D000418:Albumins; D004129:Dimethylpolysiloxanes; D011108:Polymers; D011506:Proteins; D000251:Adenosine Triphosphatases