Improving the functional expression of N-carbamoylase by directed evolution using the green fluorescent protein fusion reporter system.
Department of Biological Studies, Korea Advanced Institute of Science and Technology, Yusung-Gu, Taejon, Korea.
Hee-Sung
HS
Park
Ki-Hoon
KH
Oh
Hak-Sung
HS
Kim
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D019020:Directed Molecular Evolution / Q000379:methods*; D017930:Genes, Reporter; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D000581:Amidohydrolases; C451299:N-carbamoylamino acid amidohydrolase