Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins.
Several types of jasomonic acid (JA) derivatives, including JA--amino acid conjugates, a JA--biotin conjugate, a JA--dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA--binding proteins. These JA derivatives, excepting the JA--fluoresceine conjugate, exhibited significant biological activities in a rice seedling assay, a rice phytoalexin-inducing assay, and/or a soybean phenylalanine ammonia-lyase-inducing assay. These JA derivatives could therefore be useful probes for identifying JA--binding proteins. The activity spectra of the prepared compounds were different from each other, suggesting that different types of JA receptors were involved in the perception of JA derivatives in the respective bioassays.
Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Yusuke
Y
Jikumaru
Tadao
T
Asami
Hideharu
H
Seto
Shigeo
S
Yoshida
Tadashi
T
Yokoyama
Naomi
N
Obara
Morifumi
M
Hasegawa
Osamu
O
Kodama
Makoto
M
Nishiyama
Kazunori
K
Okada
Hideaki
H
Nojiri
Hisakazu
H
Yamane
D000596:Amino Acids / Q000737:chemistry*; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D003517:Cyclopentanes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry*; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000378:metabolism; D012275:Oryza / Q000378:metabolism; D054883:Oxylipins; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism; D011485:Protein Binding; D012717:Sesquiterpenes; D013025:Glycine max / Q000378:metabolism; D013729:Terpenes; D000094162:Phytoalexins
D000596:Amino Acids; D003517:Cyclopentanes; D005452:Fluoresceins; D015335:Molecular Probes; D054883:Oxylipins; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C011006:jasmonic acid; D001710:Biotin; D003907:Dexamethasone; D010650:Phenylalanine Ammonia-Lyase; D000094162:Phytoalexins