Halobacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine Permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description of H. salinarum.
Two rod-shaped haloarchaeal strains, A1 and A2, were isolated from a bore core from a salt mine in Austria. The deposition of the salt is thought to have occurred during the Permian period (225-280 million years ago). The 16S rDNA sequences of the strains were 97.1% similar to that of the type species of the genus Halobacterium, which was also determined in this work. Polar lipids consisted of C20-C20 derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and sulfated tetraglycosyl diether. Optimal salinity for growth was 15-17.5% NaCl; Mg++ was tolerated up to a concentration of 1 M. The DNA-DNA reassociation value of strain A1T was 25% with H. salinarum DSM 3754T and 41% with Halobacterium sp. NRC-1, respectively. Based on these results and other properties, e.g. whole cell protein patterns, menaquinone content and restriction patterns of DNA, strains A1 and A2 are members of a single species, for which we propose the name H. noricense. The type strain is A1 (DSM 15987T, ATCC BAA-852T, NCIMB 13967T). Since we present evidence that Halobacterium sp. NRC-1 is a member of H. salinarum, an emended description of H. salinarum is provided.
Fachbereich für Molekulare Biologie, Abteilung Mikrobiologie, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.
Claudia
C
Gruber
Andrea
A
Legat
Marion
M
Pfaffenhuemer
Christian
C
Radax
Gerhard
G
Weidler
Hans-Jürgen
HJ
Busse
Helga
H
Stan-Lotter
D019843:Archaeal Proteins / Q000302:isolation & purification; D001317:Austria; D001482:Base Composition; D002338:Carotenoids / Q000032:analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019766:Genes, Archaeal; D019015:Geologic Sediments / Q000382:microbiology*; D006217:Halobacterium / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019617:Halobacterium salinarum / Q000145:classification* / Q000235:genetics; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D013045:Species Specificity; D009626:Terminology as Topic; D024482:Vitamin K 2 / Q000032:analysis
D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D002338:Carotenoids; D012965:Sodium Chloride