3-nitro-3-deaza-2'-deoxyadenosine as a versatile photocleavable 2'-deoxyadenosine mimic.
A new photocleavable 2'-deoxyadenosine mimic, 3-nitro-3-deaza-2'-deoxyadenosine (NidA), was prepared and introduced into DNA fragments via its 6-O-trimethylphenyl precursor phophoramidite. Photocleavage of the resulting oligonucleotide is highly efficient in single and double strands. Hybridization properties of NidA are very similar to those of deoxyadenosine.
LEDSS, UMR5616 CNRS, ICMG FR-2607 - BP53, Université Joseph Fourier, 38041 Grenoble, France.
Caroline
C
Crey-Desbiolles
Jean
J
Lhomme
Pascal
P
Dumy
Mitsuharu
M
Kotera
D040761:Biomimetic Materials / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D003839:Deoxyadenosines / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry
C492391:3-nitro-3-deaza-2'-deoxyadenosine; D003839:Deoxyadenosines; D009841:Oligonucleotides; D004247:DNA; C058118:2'-deoxyadenosine