Tandem time-of-flight mass spectrometer for photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization (MALDI-TOF-PD-TOF) using a linear-plus-quadratic potential reflectron.
A tandem time-of-flight mass spectrometer for the study of photodissociation of biopolymer ions generated by matrix-assisted laser desorption ionization was designed and constructed. A reflectron with linear and quadratic (LPQ) potential components was used. Characteristics of the LPQ reflectron and its utility as the second stage analyzer of the tandem mass spectrometer were investigated. Performance of the instrument was tested by observing photodissociation of [M + H](+) from angiotensin II, a prototype polypeptide. Quality of the photodissociation tandem mass spectrum was almost comparable to that of the post-source decay spectrum. Monoisotopic selection of the parent ion was possible, which was achieved through the ion beam-laser beam synchronization. General theoretical considerations needed for a successful photodissociation of large biopolymer ions are also presented.
National Creative Research Initiative Center for Control of Reaction Dynamics and School of Chemistry, Seoul National University, Seoul 151-742, South Korea.
Joo Yeon
JY
Oh
Jeong Hee
JH
Moon
Myung Soo
MS
Kim
D000803:Angiotensin I / Q000737:chemistry; D000804:Angiotensin II / Q000737:chemistry; D001704:Biopolymers / Q000032:analysis*; D001920:Bradykinin / Q000737:chemistry; D036103:Nanotechnology; D010446:Peptide Fragments / Q000737:chemistry; D010777:Photochemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D014662:Vasoconstrictor Agents / Q000737:chemistry
D001704:Biopolymers; D010446:Peptide Fragments; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000803:Angiotensin I; D001920:Bradykinin