Antimicrobial activity of reuterin in combination with nisin against food-borne pathogens.
Antimicrobial activity of reuterin individually or in combination with nisin against different food-borne Gram-positive and Gram-negative pathogens in milk was investigated. Reuterin (8 AU/ml) exhibited bacteriostatic activity against Listeria monocytogenes, whereas its activity was slightly bactericidal against Staphylococcus aureus at 37 degrees C. Higher bactericidal activity was detected against Escherichia coli O157:H7, Salmonella choleraesuis subsp. choleraesuis, Yersinia enterocolitica, Aeromonas hydrophila subsp. hydrophila and Campylobacter jejuni. A significant synergistic effect on L. monocytogenes and a slight additive effect on S. aureus after 24 h at 37 degrees C were observed when reuterin was combined with nisin (100 IU/ml). The combination of reuterin with nisin did not enhance the antimicrobial effect of reuterin against Gram-negative pathogens.
Dpto. de Tecnología de Alimentos, INIA, Carretera de La Coruña km 7, 28040 Madrid, Spain.
Juan L
JL
Arqués
Javier
J
Fernández
Pilar
P
Gaya
Manuel
M
Nuñez
Eva
E
Rodríguez
Margarita
M
Medina
D000447:Aldehydes; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004357:Drug Synergism; D005516:Food Microbiology; D005985:Glyceraldehyde / Q000031:analogs & derivatives; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D008892:Milk / Q000382:microbiology*; D009561:Nisin / Q000494:pharmacology*; D011407:Propane; D013696:Temperature; D013997:Time Factors
D000447:Aldehydes; D000900:Anti-Bacterial Agents; D009561:Nisin; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D011407:Propane