Time-domain fluorescence lifetime imaging applied to biological tissue.
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue.
Photonics Group, Physics Department, Imperial College London, London, UK. ds.elson@imperial.ac.uk
Dan
D
Elson
Jose
J
Requejo-Isidro
Ian
I
Munro
Fred
F
Reavell
Jan
J
Siegel
Klaus
K
Suhling
Paul
P
Tadrous
Richard
R
Benninger
Peter
P
Lanigan
James
J
McGinty
Clifford
C
Talbot
Bebhinn
B
Treanor
Stephen
S
Webb
Ann
A
Sandison
Andrew
A
Wallace
Dan
D
Davis
John
J
Lever
Mark
M
Neil
David
D
Phillips
Gordon
G
Stamp
Paul
P
French
D000818:Animals; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods*; D004724:Endoscopy; D005453:Fluorescence; D006801:Humans; D008853:Microscopy