Studies on middle silkgland proteins of cocoon colour sex-limited silkworm (Bombyx mori L.) using two-dimensional polyacrylamide gel electrophoresis.
Qualitative and quantitative differences in proteins expressed in the middle silkglands of male and female silkworm larvae that differ in silk colour were investigated by high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), followed by computer assisted image analysis. About 1000 protein spots were resolved in both the sexes and most proteins were shown to be distributed in the area from 15 kDa to 70 kDa and pH 4-8. It was found that some proteins displayed higher expression in yellow cocoon, while two proteins were only expressed in female silkworm silkgland tissue through the comparison and analysis by two-D software. These proteins especially existed in female silkworm middle silkgland tissue of yellow cocoon. Furthermore, these proteins might be involved in the expression of cocoon colour phenotype
Jin
Yuan-Xiang
YX
College of Animal Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Chen
Yu-Yin
YY
Xu
Meng-Kui
MK
Jiang
Yong-Huang
YH
D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000378:metabolism*; D003116:Color; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007091:Image Processing, Computer-Assisted; D019476:Insect Proteins / Q000378:metabolism*; D007814:Larva / Q000737:chemistry / Q000378:metabolism; D008297:Male; D010641:Phenotype; D020543:Proteome; D012737:Sex Factors; D047011:Silk / Q000378:metabolism*
D019476:Insect Proteins; D020543:Proteome; D047011:Silk