Phase diagram determination to elucidate the crystal growth of the photoreaction center from Rhodobacter sphaeroides.
Solubility curves (a phase diagram) of an amorphous precipitate and the orthorhombic crystals of a membrane protein, the photoreaction center from Rhodobacter sphaeroides, were determined. With the phase diagram, the process of crystal growth of the membrane protein was elucidated within a general physicochemical framework, and justification was provided of hitherto empirically selected crystallization conditions.
Department of Biomolecular Engineering, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan.
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Odahara
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Ataka
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Katsura