Analysis of malignancy-associated DNA changes in interphase nuclei of buccal epithelium in persons with breast diseases.
OBJECTIVE
To study from the point of view of statistical and geometrical theory of pattern recognition, the peculiarities of the distribution of optical density of DNA in the interphase nuclei of mammary buccal epithelium upon pathology.


METHODS
Cytogenetic investigation of buccal smears and computer-based image analysis were used.


RESULTS
It is shown that in malignant neoplasms of the mammary glands compared with its values in benign, the optical density of DNA in the nuclei of buccal epithelium increase in a range from 0.15 to 0.30 conventional units. The sensitivity of that criterium varied from 80.5% to 96.8%, and specificity was 92.3%.


CONCLUSIONS
The method proposed may be recommended as an additional one for improvement of the diagnosis of mammary pathologies.
Boroday
Natalya
N
R.E.Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine. inst@iepor.kiev.ua
Klyushin
Dmitriy
D
Petunin
Yuriy
Y
Andrushkiw
Roman
R
D000328:Adult; D001699:Biometry / Q000379:methods; D001941:Breast Diseases / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology; D004247:DNA / Q000235:genetics*; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D007399:Interphase; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology*; D010363:Pattern Recognition, Automated; D012680:Sensitivity and Specificity
D004247:DNA