Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds.
An 8S storage globulin from buckwheat seed, which resembles the structure and features common to the vicilin-like family of seed storage proteins, was analyzed for this paper. It was found that expression of the 8S globulin gene precedes that of the 13S globulin (the main buckwheat storage protein) and starts from an early stage of buckwheat seed development (9-11 days after flowering), continuing to accumulate throughout seed development to contribute approximately 7% of total seed proteins. This protein fraction might be more interesting for biotechnological application than the 13S buckwheat legumin consisting of 23-25 kDa subunits reported to be the major buckwheat allergen. A partial cDNA was also isolated, showing high homology with cDNAs coding for vicilin-like storage proteins from various plant species, and its expression profile throughout seed development as well as in different buckwheat tissues was analyzed.
Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11001 Belgrade, Serbia and Montenegro.
Mira Dj
MDj
Milisavljević
Gordana S
GS
Timotijević
Svetlana R
SR
Radović
Jelena M
JM
Brkljacić
Miroslav M
MM
Konstantinović
Vesna R
VR
Maksimović
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000302:isolation & purification; D019612:Fagopyrum / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment
D018076:DNA, Complementary; D010940:Plant Proteins; D055314:Seed Storage Proteins; C015575:vicilin protein, plant