Posttransplant lymphoproliferative disease after allogeneic transplantation of the spleen in miniature swine.
Spleen transplantation (SpTx) was performed in miniature swine across full major histocompatibility complex barriers to study the tolerogenic effect of the spleen. This study describes the development of posttransplant lymphoproliferative disease (PTLD) after allogeneic SpTx. Recipient pigs underwent whole body irradiation (100 cGy), thymic irradiation (700 cGy), and native splenectomy (day 0), and received a 45-day course of intravenous cyclosporine (trough level 400-800 ng/ml). After SpTx, two of seven pigs developed PTLD (1 donor-type, 1 host-type). These two pigs had greater T cell depletion and higher trough levels of cyclosporine. Early changes that occurred prior to the development of clinical features of PTLD were increased porcine lymphotropic herpesvirus-1 viral loads in blood and tissues, and increased numbers of leukocytes, B cells, and total serum IgM. PTLD can occur after allogeneic SpTx in swine. This model may be useful in studies of the pathogenesis of PTLD.
Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149, 13th Street, Boston, MA 02129, USA.
Frank J M F
FJ
Dor
Karen E
KE
Doucette
Nicolas J
NJ
Mueller
Robert A
RA
Wilkinson
Junaid A
JA
Bajwa
Isabel M
IM
McMorrow
Yau-Lin
YL
Tseng
Kenji
K
Kuwaki
Stuart L
SL
Houser
Jay A
JA
Fishman
David K C
DK
Cooper
Christene A
CA
Huang
D000818:Animals; D004195:Disease Models, Animal; D006650:Histocompatibility Testing; D007958:Leukocyte Count; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*; D011183:Postoperative Complications / Q000175:diagnosis*; D013552:Swine; D013556:Swine, Miniature; D014184:Transplantation, Homologous / Q000009:adverse effects*