The effect of a two-hour, room temperature incubation of human spermatozoa in TEST-yolk buffer on the rate of fertilization in vitro.
OBJECTIVE
To reassess the use of TEST-yolk buffer (TYB) in an in vitro fertilization (IVF) program by comparing fertilization rates achieved in a glucose-free cleavage medium by the standard IVF preparation of sperm versus a 2-h, room temperature incubation of sperm in TYB.


METHODS
Oocytes collected for IVF were randomly split into two groups and inseminated with either TYB-treated sperm or IVF-prepared sperm.


METHODS
Stanford Reproductive Endocrinology and Infertility Center.


METHODS
Fifty couples undergoing IVF with at least 10 mature oocytes.


METHODS
Fertilization rates in vitro.


RESULTS
Fertilization rates were significantly higher (p = 0.015) with TYB treatment. The average 2PN fertilization rate was 49.6% (188/379) for the IVF group and 57.4% (221/385) in the IVF with TYB group.


CONCLUSIONS
A 2-h, room temperature incubation of sperm in TYB produces significantly higher 2PN fertilization rates as compared to standard IVF preparation of sperm in a current generation cleavage medium.
Jensen
Julie R
JR
California Polytechnic State University, San Luis Obispo, California, USA.
Walker
John H
JH
Milki
Amin A
AA
Westphal
Lynn
L
Behr
Barry
B
D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008297:Male; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D011256:Pregnancy Outcome; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014325:Tromethamine / Q000737:chemistry*
D003470:Culture Media; C077847:TES-Tris yolk buffer; D014325:Tromethamine; D005947:Glucose