Molecular cloning and characterization of a cDNA encoding the Paracoccidioides brasiliensis 135 ribosomal protein.
A 630 bp cDNA encoding an L35 ribosomal protein of Paracoccidioides brasiliensis, designated as Pbl35, was cloned from a yeast expression library. Pbl35 encodes a polypeptide of 125 amino acids, with a predicted molecular mass of 14.5 kDa and a pI of 11.0. The deduced PbL35 shows significant conservation in respect to other described ribosomal L35 proteins from eukaryotes and prokaryotes. Motifs of ribosomal proteins are present in PbL35, including a bipartite nuclear localization signal (NLS) that could be related to the protein addressing to the nucleolus for the ribosomal assembly. The mRNA for PbL35, about 700 nucleotides in length, is expressed at a high level in P. brasiliensis. The PbL35 and the deduced amino acid sequence constitute the first description of a ribosomal protein in P. brasiliensis. The cDNA was deposited in GenBank under accession number AF416509.
Jesuino
Rosália S A
RS
Laboratório de Biologia Molecular, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Pereira
Maristela
M
Felipe
M Sueli S
MS
Azevedo
Maristella O
MO
Soares
Célia M A
CM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019913:Nuclear Localization Signals / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010228:Paracoccidioides / Q000235:genetics*; D039104:RNA 3' Polyadenylation Signals / Q000235:genetics; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000502:physiology
D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D004271:DNA, Fungal; D019913:Nuclear Localization Signals; D012331:RNA, Fungal; D012333:RNA, Messenger; D012269:Ribosomal Proteins