Key differences between lateral and apical branching in hyphae of Neurospora crassa.
We examined in fine detail growth kinetics and intracellular events during lateral and apical branching in hyphae of Neurospora crassa. By high-resolution video-enhanced light microscopy, we found remarkable differences in the events preceding lateral vs apical branching. While apical branching involved a significant disturbance in the apical growth of the parental hypha, lateral branching occurred without any detectable alterations in the growth of the parental hypha. Prior to the emergence of a lateral branch, an incipient Spitzenkörper was formed about 12-29 microm behind the apex. Lateral branch formation did not interfere with the elongation rate of the primary hypha, the shape of its apex or the behavior of its Spitzenkörper. In sharp contrast, apical branching was preceded by marked changes in physiology and morphology of the parental hypha and by a sharp drop in elongation rate. The sequence involved a cytoplasmic contraction, followed by a retraction, dislocation, and disappearance of the Spitzenkörper; hyphal elongation decreased sharply and a transient phase of isotropic growth caused the hyphal apex to round up. Growth resumed with the formation of two or more apical branches, each one with a Spitzenkörper formed by gradual condensation of phase-dark material (vesicles) around an invisible nucleation site. The observed dissimilarities between lateral and apical branching suggest that these morphogenetic pathways are triggered differently. Whereas apical branching may be traced to a sudden discrete disruption in cytoplasmic organization (cytoplasmic contraction), the trigger of lateral branching probably stems from the subapical accumulation of wall precursors (presumably vesicles) reaching a critical concentration.
Department of Plant Pathology, University of California, Riverside 92521-0122, USA.
Meritxell
M
Riquelme
Salomon
S
Bartnicki-Garcia
D002473:Cell Wall / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D025301:Hyphae / Q000166:cytology* / Q000254:growth & development*; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video; D009024:Morphogenesis; D009492:Neurospora crassa / Q000166:cytology* / Q000254:growth & development*