Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.
Mitogenic activity of UV-inactivated influenza viruses in cord blood lymphocytes (CBL), as measured by cytokine release, was investigated. Using prototype viruses of subtype H3N2 (A/Aichi/68), H2N2 (A/Japan/57), and H1N1 (A/Puerto Rico/34) for influenza A virus, and B/Lee/40 for influenza B virus, the results indicated that both Th1 and Th2 cytokines were induced. Stimulation indices were significantly higher for IFNgamma, IL-4 and IL-10 by influenza A viruses than by influenza B virus. Stimulation indices for IL-2 and IL-6 were lower, as these two cytokines were spontaneously released by cord blood lymphocytes in culture. Alignment of the amino acid sequences of the HA for the viruses used in this study indicated that influenza B virus lacked sequence homology to the antigenic sites identified for influenza A virus. Therefore, the antigenic sites may play a role in the mitogenic property, and cord blood lymphocytes could provide a system to compare this property for clinical isolates of influenza virus.
Divanovic
Senad
S
Department of Microbiology and Molecular Genetics, Oklahoma State University, 306 Life Science East, Stillwater, OK 74078, USA.
Lai
Alexander C K
AC
D000595:Amino Acid Sequence; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000276:immunology* / Q000528:radiation effects; D016415:Sequence Alignment
D016207:Cytokines