Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b.
Methylosinus trichosporium OB3b oxidized methane to methanol in the presence of a high concentration of Cu2+. Further oxidation of methanol to formaldehyde was prevented by adding 200 mM NaCl which acted as a methanol dehydrogenase H inhibitor. The bacterium, 0.6 mg dry cell ml(-1), in methane/air (1:4, v/v) at 25 degrees C in 12.9 mM phosphate buffer (pH 7) containing 20 mM sodium formate and 200 mM NaCl accumulated 7.7 mM methanol over 36 h.
Lee
Sang Gui
SG
Department of Biomaterials, Chosun University, Gwangju 501-759, Korea.
Goo
Jae Hwan
JH
Kim
Hee Gon
HG
Oh
Jeong-Il
JI
Kim
Young Min
YM
Kim
Si Wouk
SW
D018929:Cell Culture Techniques / Q000379:methods*; D003300:Copper / Q000494:pharmacology; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D020602:Methylosinus trichosporium / Q000254:growth & development / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride; D003300:Copper; D010088:Oxidoreductases; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane; D000432:Methanol