[Cytogenetic maps of lampbrush chromosomes of newts of the genus Pleurodeles: an algorithm of lampbrush chromosome identification in Pleurodeles waltl by immunocytochemical marker-loop staining with polyclonal anti-Ro52 antisera].
Our work was aimed at developing a simple and effective method of identification of most or all chromosomes of Pleurodeles newts. To this end, we used DAPI staining of the chromomeres of newt lampbrush chromosomes and immunochemical reactions between the ribonucleoprotein (RNP) marker loops and polyclonal antibodies against human zinc-finger protein Ro52 (52-kDa Ro/SS-A). A method has been developed to obtain newt lampbrush chromosome preparations. Cytological maps of P. waltl chromosomes (Spanish population/subspecies) showing distributions of chromomeres and marker loops along the chromosome length were constructed.
Vishniakova
N M
NM
Lacroix
J-C
JC
Rodionov
A V
AV
D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D007106:Immune Sera; D007150:Immunohistochemistry; D007621:Karyotyping / Q000379:methods*; D010999:Pleurodeles / Q000235:genetics* / Q000276:immunology; D012261:Ribonucleoproteins / Q000032:analysis* / Q000276:immunology; D000099207:SS-A Antigen
D015415:Biomarkers; D007106:Immune Sera; D012261:Ribonucleoproteins; D000099207:SS-A Antigen