Stereospecific effects of catechin isomers on insulin induced lipogenesis in 3T3-L1 cells.
We studied the influence of (+)- and (-)-catechin contained in green tea on insulin induced lipogenesis in 3T3-L1 cells. In 14 days of culture with insulin, the intracellular triglyceride concentration and the activities of glycerophosphate dehydrogenase, a marker of adipose conversion, increased. The addition of 0.02 mg/ml (+)-isomer stimulated the accumulation of triglyceride induced by insulin, but the addition of the same concentration of (-)-isomer inhibited lipogenesis. The activities of glycerophosphate dehydrogenase were changed by (+)- and (-)-catechin in the same direction as the corresponding changes in triglyceride accumulation. These data suggest a biological significance of catechins, with opposite effects on lipid metabolism depending on the isomer.
Mochizuki
Miyako
M
Department of Health and Nutrition School of Health and Human Life, Nagoya Bunri University, Inazawa, Japan.
Hasegawa
Noboru
N
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin; D007536:Isomerism; D051379:Mice; D008517:Phytotherapy; D013662:Tea; D014280:Triglycerides / Q000378:metabolism*
D000960:Hypolipidemic Agents; D007328:Insulin; D013662:Tea; D014280:Triglycerides; D002392:Catechin