The influence of genotypic variation on metabolite diversity in populations of two endophytic fungal species.
The relationship between metabolite production and genotypic diversity in two endophytic fungi was investigated. We selected populations of Cylindrocarpon destructans and Heliscus lugdunensis from the roots of a single tree. A total of 49 isolates of both species were selected and classified by simple genotypic tests (random amplified polymorphic DNA analysis and rDNA-ITS sequencing). In a blind test, the ability of these fungi to produce natural products was tested by ethyl acetate extraction of hyphae and culture filtrates, followed by high-performance liquid chromatography analysis (HPLC). A positive relationship was found between genotype classification and the pattern of natural products produced by a given isolate. To test the robustness of this correlation, a discriminate selection procedure was carried out by collecting fungal isolates from a second site and selecting a sub-set of the population, on the basis of genotypic variability. This sub-set of fungal isolates produced greater numbers of unique metabolites than those selected indiscriminately.
School of Biological Sciences, Washington Singer Laboratories, Perry Road, University of Exeter, EX4 4QG, UK.
Fabian A
FA
Seymour
James E
JE
Cresswell
P Jack
PJ
Fisher
Hilary M
HM
Lappin-Scott
Hubert
H
Haag
Nicholas J
NJ
Talbot
D029661:Alnus / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D006999:Hypocreales / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer