Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications.
Recent developments in single molecule force spectroscopy (SMFS) allow direct observation and measurements of forces that hold protein-DNA complexes together. Furthermore, the mechanics of double-stranded (ds) DNA molecules in the presence of small binding ligands can be detected. The results elucidate molecular binding mechanisms and open the way for ultra sensitive and powerful biosensor applications.
Ros
Robert
R
Experimental Biophysics, Faculty of Physics, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany. robert.ross@physik.uni-bielefeld.de
Eckel
Rainer
R
Bartels
Frank
F
Sischka
Andy
A
Baumgarth
Birgit
B
Wilking
Sven David
SD
Pühler
Alfred
A
Sewald
Norbert
N
Becker
Anke
A
Anselmetti
Dario
D
D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*; D004548:Elasticity; D008024:Ligands; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D009690:Nucleic Acid Conformation; D010812:Physical Stimulation / Q000295:instrumentation / Q000379:methods; D011485:Protein Binding; D011487:Protein Conformation; D013314:Stress, Mechanical
D004268:DNA-Binding Proteins; D008024:Ligands; D004247:DNA