Ca2+ dependence and inhibitory effects of trifluoperazine on plasma membrane ATPase of Thermoactinomyces vulgaris.
Ca2+ enhanced the plasma membrane Ca(2+)-ATPase (PMCA) specific activities in wild-type strain 1227 and mutant strains 1278, 1286, and 1261 of Thermoactinomyces vulgaris. The Ca(2+)-ATPase specific activities showed marked increase with increasing concentrations of Ca2+ added in the form of CaCl2 in the culture medium and reached the optimum values at 0.6 mM in strains 1227, 1278, and 1286 and at 0.7 mM in strain 1261 of T. vulgaris. Trifluoperazine, a specific blocker of calmodulin, when added in vivo at concentrations of 2 microM and 8 microM along with the respective optimal concentrations of Ca2+, decreased the PMCA-specific activities to a low level in a dose-dependent manner. The results of the present investigation suggest the presence of a Ca(2+)-dependent protein activator (CaDPA) in the microenvironment constituting this enzyme; and such Ca(2+)-modulated protein has been assigned to play an important role in the enhancement of PMCA levels in this aerobic, spore-forming, thermophilic actinomycete.
Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi-110007, India.
Kartiki
K
Bhatnagar
Ved Pal
VP
Singh
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002122:Calcium Chloride / Q000378:metabolism / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D018492:Dopamine Antagonists / Q000494:pharmacology; D008848:Micromonosporaceae / Q000201:enzymology* / Q000235:genetics; D014268:Trifluoperazine / Q000737:chemistry / Q000494:pharmacology*
D002147:Calmodulin; D018492:Dopamine Antagonists; D014268:Trifluoperazine; D000251:Adenosine Triphosphatases; D002122:Calcium Chloride; D002118:Calcium