A comparison of 14 antibodies for the biochemical detection of the cystic fibrosis transmembrane conductance regulator protein.
Interest in the biochemical detection of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) protein followed soon after cloning of the gene and prediction of the protein structure. Ever since, antibodies (Abs) have been produced and used to detect CFTR in both heterologously and endogenously expressing cells and tissues. Although designed to be sensitive and specific, these Abs produce, in most cases, unsatisfactory results when used for the biochemical detection of CFTR either by Western blot or by immunoprecipitation. The lack of Abs that can reliably detect the CFTR protein is a major constraint to studies of CF. We compared 14 different Abs for their ability to detect CFTR in both stably transfected and endogenously expressing cell lines.
Farinha
Carlos M
CM
Centre of Human Genetics, National Institute of Health, Avenida Padre Cruz, 1649-016 Lisboa, Portugal.
Mendes
Filipa
F
Roxo-Rosa
Mónica
M
Penque
Deborah
D
Amaral
Margarida D
MD
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003550:Cystic Fibrosis / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000276:immunology*; D006801:Humans; D047468:Immunoprecipitation
D000906:Antibodies; C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator