Facile synthesis of membrane-embedded peptides utilizing lipid bilayer-assisted chemical ligation.
Lipid bilayer-assisted chemical ligation between thiolester and N-terminal cysteine peptides has been developed with successful application to the synthesis of membrane protein segments possessing both two transmembrane and one extracellular regions.
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. aotaka@pharm.kyoto-u.ac.jp
Akira
A
Otaka
Satoshi
S
Ueda
Kenji
K
Tomita
Yoshiaki
Y
Yano
Hirokazu
H
Tamamura
Katsumi
K
Matsuzaki
Nobutaka
N
Fujii
D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism
D004952:Esters; D008024:Ligands; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D013438:Sulfhydryl Compounds; D003545:Cysteine