Mitogenic properties of pokeweed lectin-D isoforms on human peripheral blood lymphocytes: non-mitogen PL-D1 and mitogen PL-D2.
We investigated native structures and mitogenic properties of pokeweed lectin-D isoforms (PL-D1 and -D2) on human peripheral blood lymphocytes along with other isolectins (PL-A to -C). Both native PL-D isoforms appeared to behave as monomers. PL-D2 proliferated the lymphocytes like PL-C, whereas PL-D1 had no mitogenicity. PL-D1 acquired mitogenic activity after trimming of the C-terminal dipeptide.
Laboratory of Protein Chemistry & Engineering, Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. kenichi@net.nagasaki-u.ac.jp
Kenichi
K
Yamaguchi
Mizuki
M
Uechi
Yoshinori
Y
Katakura
Tatsuya
T
Oda
Masatsune
M
Ishiguro
D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008938:Mitosis / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000276:immunology / Q000494:pharmacology*; D020033:Protein Isoforms; D054788:Ribosome Inactivating Proteins; D013778:Tetrazolium Salts / Q000494:pharmacology
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D011043:Pokeweed Mitogens; D020033:Protein Isoforms; D013778:Tetrazolium Salts; C104076:pokeweed lectin D; D054788:Ribosome Inactivating Proteins