Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir-Blodgett lithography.
Topographically patterned surfaces are known to influence cellular behavior in a controllable manner. However, the relatively large surface areas (several cm2) required for many biomaterial applications are beyond the practical limits of traditional lithography. Langmuir-Blodgett lithography, a recently developed method, was used to fabricate regularly spaced grooves of different depths (50 and 150 nm) with a periodicity of 500 nm over several square centimeter on silicon surfaces. These topographies were transferred into polystyrene surfaces by means of nanoimprinting. Primary osteoblasts were cultured on the patterned polymer surfaces. They were observed to align, elongate and migrate parallel to the grooves. The combination of Langmuir-Blodgett lithography with nanoimprinting enables the fabrication of large, nanostructured surface areas on a wide spectrum of different biomaterials. Osteoblasts show a significant anisotropic behavior to these surfaces, which can enhance cell settlement on the surface or be used to direct tissue generation on the biomaterial interface.
Lenhert
Steven
S
Klinik und Poliklinik für Mund- und Kiefer-Gesichtschirurgie, Universitätsklinikum Münster, Waldeyerstrasse 30, Münster D-48149, Germany. lenhert@uni-muenster.de
Meier
Marie-Beatrice
MB
Meyer
Ulrich
U
Chi
Lifeng
L
Wiesmann
Hans Peter
HP
D000185:Actinin / Q000032:analysis; D000199:Actins / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002465:Cell Movement; D048430:Cell Shape; D003599:Cytoskeleton / Q000648:ultrastructure; D005353:Fibronectins / Q000032:analysis; D016023:Integrins / Q000032:analysis; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D011137:Polystyrenes / Q000494:pharmacology*; D012825:Silicon; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation; D016596:Vinculin / Q000032:analysis
D000199:Actins; D001672:Biocompatible Materials; D005353:Fibronectins; D016023:Integrins; D011137:Polystyrenes; D000185:Actinin; D016596:Vinculin; D012825:Silicon