[The age-related specificity of site-oriented immunity in respiratory-syncytial virus infection].
ELISA test systems were designed on the basis of synthetic peptides (SP) simulating the primary structure of functionally significant epitopes of the respiratory and cyncyntial virus (RCV) F-protein for the purpose of investigating the structure and age-related peculiarities of humoral immunity in respect to separate epitopes of RCV F-protein. One of them (221-232) simulates a part of RCV "virus-neutralizing domain" and another one (479-491) is highly important for the fusion mechanisms. New SP-based ELISA were used to examine pair sera in 159 patients with documented RCV infection including children, aged up to 3 years and 3 to 15, and adults. The activity of anti-RCV antibodies to SP was found to be significantly lower in children aged up to 3 years versus the older children and especially versus the adults. The virus neutralizing and, to a greater extent, fusion-inhibiting activities of antibodies were increasing with age, which collated with the results of detecting the antibodies to SP by immune-enzyme assay. The results testify to synchronism of formation of antibodies to different epitopes of the RCV F-protein. The shaping-up of antibodies with the above SP could denote the protective properties of humoral immunity, which justifies the use of the SP-based ELISA in its analysis, especially, in babies as well as in different-type immunodeficiency and immunopathology conditions.
Krivitskaia
V Z
VZ
Sominina
A A
AA
Vlasov
G P
GP
Sukhovetskaia
V F
VF
Golovanova
A K
AK
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007223:Infant; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017434:Protein Structure, Tertiary; D018357:Respiratory Syncytial Virus Infections / Q000097:blood*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D013045:Species Specificity; D014760:Viral Fusion Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D014760:Viral Fusion Proteins; D014764:Viral Proteins