Simultaneous quantification of opiates, cocaine, and metabolites in hair by LC-APCI-MS/MS.
A quantitative LC-APCI-MS/MS method for simultaneous measurement of opiates, cocaine, and metabolites in hair was developed and validated. Cocaine and opiates were extracted from pulverized hair via sonication in methanol at 37 degrees C for 3 h. Samples were cleaned up using solid-phase extraction. LC separation was achieved in 20 min with identification and quantification by selected reaction monitoring. Calibration by linear regression analysis utilized deuterated internal standards and a weighting factor of 1/x (R(2) > 0.998). Limits of quantification (LOQ) ranged from 17 to 50 pg/mg for cocaine and metabolites and were 83 pg/mg for opiates. Standard curves were linear from the LOQ to 5000 pg/mg for cocaine and metabolites, except benzoylecgonine (2500 pg/mg). Opiate standard curves were linear from the LOQ to 12500 pg/mg. Accuracy ranged from 84 to 115% for all quantitative analytes. Precision, % relative standard deviation, was less than 11.0% for all analytes. Methanolic sonication produced less than 5% hydrolysis of cocaine and 6-acetylmorphine. The method should be useful for studying cocaine and opiate distribution into hair.
Scheidweiler
Karl B
KB
Chemistry and Drug Metabolism Section, Intramural Research Program, National Institute on Drug Abuse, 5500 Nathan Shock Drive, Baltimore, Maryland 21224, USA.
Huestis
Marilyn A
MA
D003042:Cocaine / Q000032:analysis* / Q000493:pharmacokinetics; D006197:Hair / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009294:Narcotics / Q000032:analysis* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D014465:Ultrasonics
D009294:Narcotics; D003042:Cocaine