Antisense Tiam1 down-regulates the invasiveness of 95D cells in vitro.
As a specific guanine nucleotide exchange factor of Rac1, Tiam1 (T-lymphoma invasion and metastasis inducing protein 1) is involved in a number of cellular events, such as cytoskeleton reorganization, cell adhesion, and cell migration. Since Tiam1 was implicated in the invasion and metastasis of T-lymphoma cells and breast tumor cells, we compared the expression level of Tiam1 in two human giant-cell lung carcinoma cell strains with high or low metastasis potential, and found that Tiam1 expression level in high-metastatic 95D cells was higher than that in low-metastatic 95C cells. To further confirm the role of Tiam1 in invasion and metastasis, we constructed the antisense Tiam1 expression plasmid (pcDNA3-anti-Tiam1), which was transfected into 95D cells. A stable transfected clone with decreased Tiam1 expression was screened and selected for further research. Transwell assay showed that down-regulation of endogenous Tiam1 by anti-Tiam1 can reduce the in vitro invasiveness of 95D cells. Our results suggested that Tiam1 signaling contributed to the invasion and metastasis of the human giant-cell lung carcinoma cells.
Department of Molecular Genetics, Shanghai Medical College, Fudan University and Key Laboratory of Molecular Medicine, Ministry of National Education, Shanghai 200032, China.
Min
M
Hou
Li
L
Tan
Xia
X
Wang
Yun-Song
YS
Zhu
D001483:Base Sequence; D018286:Carcinoma, Giant Cell / Q000235:genetics / Q000503:physiopathology; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000235:genetics; D015536:Down-Regulation; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000503:physiopathology; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000503:physiopathology*; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology*; D015398:Signal Transduction; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; D014162:Transfection
D016373:DNA, Antisense; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human