Lowered glucose suppressed the proliferation and increased the differentiation of murine neural stem cells in vitro.
Cerebral ischemia is known to activate endogenous neural stem cells (NSCs), but its mechanisms remain unknown. Since lowered glucose supply seems to mediate ischemic actions, we examined the effect of low glucose on NSC activities in vitro. Low glucose applied during the proliferation period diminished EGF-induced proliferation of NSCs without affecting subsequent differentiation, but low glucose directly exposed during the differentiation period facilitated the differentiation of NSCs into neurons and astrocytes. These findings suggest that low glucose facilitated NSC differentiation, but it diminished NSC proliferation. Moreover, the effect of low glucose may be dependent on the timing of application.
Division of Neurobiology and Behavior, Department of Translational Medical Science, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Nobutaka
N
Horie
Takahiro
T
Moriya
Masato
M
Mitome
Naoki
N
Kitagawa
Izumi
I
Nagata
Kazuyuki
K
Shinohara
D000818:Animals; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D009420:Nervous System / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D001973:Bromodeoxyuridine; D005947:Glucose