Trace analysis of rapamycin in human blood by micellar electrokinetic chromatography.
A capillary electrophoretic method with UV detection at 278 nm has been developed for analysis of the immunosuppressant rapamycin (sirolimus) in human blood at low microgram per liter levels. Separation has been achieved in an acidic carrier electrolyte containing sodium dodecylsulfate and 30% (v/v) acetonitrile. For sample clean-up and preconcentration, an off-line solid-phase extraction step using a silica-based reversed-phase material and an on-capillary focussing technique were employed. The latter allows the injection of increased sample volumes without excessive band broadening. Although this new method is less sensitive than existing liquid chromatographic procedures combined with mass spectrometry, it is fully suited to routine analysis of rapamycin in blood from patients treated with this drug. Last but not least the low costs make it an attractive alternative to established methods.
Institute for Analytical Chemistry, Johannes-Kepler-University Linz, Altenbergerstrasse 69, 4040 Linz, Austria.
Wolfgang
W
Buchberger
Matthias
M
Ferdig
Rudolf
R
Sommer
Thuy Diep Thanh
TD
Vo
D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D006801:Humans; D008823:Micelles; D008968:Molecular Conformation; D012015:Reference Standards; D012680:Sensitivity and Specificity; D020123:Sirolimus / Q000097:blood* / Q000737:chemistry* / Q000592:standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods / Q000592:standards
D008823:Micelles; D020123:Sirolimus