Detection of Haemophilus influenzae type b by real-time PCR.
A real-time PCR assay targeting the capsulation locus of Haemophilus influenzae type b (Hib) was developed. The linear detection range was from 1 to 10(6) microorganisms per reaction mixture. No H. influenzae other than Hib or any other control bacteria typically found in the upper respiratory tract was detected.
Division of Infectious Diseases, University Children's Hospital of Zurich, Steinwiesstr. 75, CH-8032 Zurich, Switzerland.
Adrian
A
Marty
Oliver
O
Greiner
Philip J R
PJ
Day
Sibylle
S
Gunziger
Kathrin
K
Mühlemann
David
D
Nadal
D001483:Base Sequence; D003199:Computer Systems; D017931:DNA Primers; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers