Dextransucrase mutants of Leuconostoc mesenteroides BI-08 strain.
We have isolated mutants constitutive for dextransucrase from Leuconostoc mesenteroides--BI-08 using ethyl methane sulfonate. The mutants produced mainly extracellular dexrtansucrase on glucose media with higher activity (3.5-4 times) than what the parental strain produced on glucose and 2 times more than what the parental strain produced on sucrose. Based on endo-dextranase from Penicillium hydrolysis, mutant BI-08 dextransucrases produced slightly different dextrans when they were elaborated on a glucose medium and on a sucrose medium Differences in viscosity, water solubility, susceptibility to endo-dextranase hydrolysis dextrans elaborated by different mutants grown on glucose media and sucrose media were found. SDS-PAGE analysis shows that the molecular masses of the dextransucrases from the studing mutants were 130-180 kDa.
Iliev
Ilia
I
Department of Biochemistry & Microbiology, Plovdiv University, 24, Tzar Assen, Str., Plovdiv-4000, Bulgaria.
Filibeva
Galina
G
Ivanova
Iskra
I
D018533:Biomass; D003470:Culture Media; D003910:Dextranase / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005947:Glucose; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D007934:Leuconostoc / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D009153:Mutagens; D009154:Mutation; D013379:Substrate Specificity; D013395:Sucrose
D003470:Culture Media; D009153:Mutagens; D013395:Sucrose; D005020:Ethyl Methanesulfonate; D005964:Glucosyltransferases; C020025:dextransucrase; D003910:Dextranase; D005947:Glucose