Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs.
Fluorescent cis-platinum compounds that react with the N7 atom of guanine are useful for labeling nucleic acids influorescence hybridization applications. Here we report that cyanin (CyN) cis-platinum labeling of DNA samples for array comparative genomic hybridizations (arrayCGH) can be achieved reproducibly and reliably. We demonstrate that degrees of labeling of approximately 1% of all nucleotides in test and reference DNA samples with CyN3- and CyN5-cis-platinum produces arrayCGH signal-to-background ratios ranging from 30 to 40. The arrayCGH results achieved during analyses of mouse and human tumor samples were comparable to those achieved using enzymatic labeling. Thus, we conclude that Cy-cis-platinum labeling is an alternative to enzymatic labeling for arrayCGH.
Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. a.k.raap@lumc.nl
Anton K
AK
Raap
Marja J M
MJ
van der Burg
Jeroen
J
Knijnenburg
Eric
E
Meershoek
Carla
C
Rosenberg
Joe W
JW
Gray
Joop
J
Wiegant
J Graeme
JG
Hodgson
Hans J
HJ
Tanke
D002232:Carbocyanines / Q000737:chemistry*; D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
C478828:BEBO cyanine dye; D002232:Carbocyanines; D004247:DNA; D002945:Cisplatin