Bradyrhizobium betae sp. nov., isolated from roots of Beta vulgaris affected by tumour-like deformations.
Some varieties of sugar beet, Beta vulgaris, cultivated in northern Spain have large deformations that resemble the tumours produced by Agrobacterium species. In an attempt to isolate the agent responsible for these deformations, several endophytic slow-growing bacterial strains were isolated, the macroscopic morphology of which resembled that of Bradyrhizobium species. These strains were not able to produce tumours in Nicotiana tabacum plants and, based on phylogenetic analysis of their 16S rRNA, they are closely related to the genus Bradyrhizobium. Phenotypic and molecular characteristics of these strains revealed that they represent a species different from all Bradyrhizobium species previously described. Sequence analysis of the 16S-23S rDNA intergenic spacer region indicated that these novel strains form a homogeneous group, related to Bradyrhizobium japonicum, Bradyrhizobium liaoningense and Bradyrhizobium yuanmingense. DNA-DNA hybridization confirmed that these strains represent a novel species of the genus Bradyrhizobium, for which the name Bradyrhizobium betae sp. nov. is proposed. The type strain is PL7HG1T (=LMG 21987T=CECT 5829T).
Rivas
Raúl
R
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Willems
Anne
A
Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Palomo
José Luis
JL
Centro Regional de Diagnóstico de la Junta de Castilla y León, Salamanca, Spain.
García-Benavides
Pablo
P
Centro Regional de Diagnóstico de la Junta de Castilla y León, Salamanca, Spain.
Mateos
Pedro F
PF
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Martínez-Molina
Eustoquio
E
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Gillis
Monique
M
Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Velázquez
Encarna
E
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
D015373:Bacterial Typing Techniques; D027461:Beta vulgaris / Q000382:microbiology*; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D010941:Plant Tumors / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D013030:Spain
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S