[Screening of cDNA library of Schistosoma japonicum with sera from rabbits vaccinated with ultraviolet-attenuated schistosomula].
OBJECTIVE
To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae.


METHODS
Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced.


RESULTS
Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.


CONCLUSIONS
Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.
Li
Xiao-hong
XH
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Liu
Shu-xian
SX
Song
Guang-cheng
GC
Xu
Yu-xin
YX
Cao
Jian-ping
JP
Chen
Jia-xu
JX
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017238:Genes, Helminth; D007106:Immune Sera / Q000276:immunology*; D007814:Larva / Q000276:immunology / Q000528:radiation effects; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology*; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology
D018076:DNA, Complementary; D007106:Immune Sera; D014613:Vaccines, Attenuated