Selective capture of prostatic basal cells and secretory epithelial cells for proteomic and genomic analysis.
Basal cells play an undefined role in signaling the growth and differentiation of normal secretory epithelial cells in the human prostate. Because basal cells disappear during malignant transformation, we hypothesize that loss of basal cell function may have a permissive role in progression of prostate intraepithelial neoplasia into invasive carcinoma. We describe an immuno-laser capture microdissection approach to selectively capture basal cells. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we identified several protein candidates selectively expressed in microdissected basal cells. We also demonstrate that the RNA derived form this technique is an excellent source for gene-array studies. Thus, we provide evidence that proteomic and microgenomic techniques can be successfully applied to investigate the expression profiles of basal and secretory cells after immuno-capture.
Diaz
Jose I
JI
Department of Microbiology, Eastern Virginia Medical School, Norfolk, USA.
Cazares
Lisa H
LH
Corica
Alberto
A
John Semmes
O
O
D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D007118:Immunoassay; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry; D042282:Microdissection / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000032:analysis; D015398:Signal Transduction
D012313:RNA