The presence of nicotinamide adenine dinucleotide-independent Haemophilus paragallinarum in México.
Two isolates of Haemophilus paragallinarum were obtained from a layer chicken in Mexico. The isolates were confirmed as H. paragallinarum by polymerase chain reaction and conventional biochemical identification. The isolates were nicotinamide adenine dinucleotide (NAD) independent-growing on blood agar without the need of a nurse colony as well as on a complex medium that lacked both NAD and chicken serum. Both isolates were pathogenic, causing the typical clinical signs of infectious coryza in susceptible chickens. One isolate was Page serovar B/Kume serovar B-1 and the other isolate was Page serovar C/Kume serovar C-2. The isolates were associated with a field outbreak that involved an egg drop of 20% over a 3-wk period and a doubling of weekly mortality (from 0.1% to 0.2%). This is the first report of NAD-independent H. paragallinarum outside South Africa and is the first time that NAD-independent H. paragallinarum of serovar B has been reported.
Laboratorios Avilab, Porcicultores 80 Tepatitlan, Jalisco, CP 47600, Mexico.
A J
AJ
García
E
E
Angulo
P J
PJ
Blackall
A M
AM
Ortiz
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002645:Chickens / Q000382:microbiology*; D006192:Haemophilus Infections / Q000382:microbiology*; D044134:Haemophilus paragallinarum / Q000302:isolation & purification* / Q000378:metabolism; D008800:Mexico; D009243:NAD / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary
D009243:NAD