Crystallization and preliminary X-ray analysis of a vanadium-dependent peroxidase from Ascophyllum nodosum.
Peroxidase from the brown alga Ascophyllum nodosum, a non-heme vanadium-dependent haloperoxidase, has been purified to homogeneity and crystallized from ammonium sulfate solutions in a form suitable for X-ray diffraction analysis. The crystals have been grown by the vapour-diffusion technique using the sitting-drop method. X-ray diffraction studies show that the crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with a = b = 114.3 and c = 276.0 A. The crystals diffract to at least 2.4 A resolution.
GBF, Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany.
M
M
Weyand
H J
HJ
Hecht
H
H
Vilter
D
D
Schomburg