[Level of tissue plasminogen activator protein in pulmonary artery in acute pulmonary thromboembolism in rabbit].
OBJECTIVE
To study the changes in tissue plasminogen activator(t-PA) protein in pulmonary artery and its clinical significance after acute pulmonary thromboembolism (PTE).


METHODS
Thirty rabbits were randomly divided into four groups after replicating a model of acute PTE in rabbit by thrombi occlusion method. Specimens were obtained from both normal and morbid pulmonary artery 3, 8 and 24 hours after APE, and protein contents of t-PA were determined using immunohistochemical method.


RESULTS
A few endothelial cells and smooth muscle cells of the normal pulmonary artery were positive for t-PA. After 3 hours of PTE, there was no significant changes in t-PA positive stain among embolismic, non-embolismic and normal pulmonary artery. After 8 and 24 hours of PTE, strong positive staining was found in the residual endothelial cells and a part of smooth muscle cells (all P<0.01).


CONCLUSIONS
There is significantly strong positive staining for t-PA in the pulmonary artery wall after pulmonary embolism, implying that the local fibrinolysis activity was enhanced, and it might be helpful for lysis of the embolus.
TianJin Thoracic Hospital, TianJin 300051, China.
Jing-xia
JX
Zhang
Gen-yi
GY
Sun
Yong-li
YL
Chen
Pei-xian
PX
Wang
Zhi-ming
ZM
Yuan
Yu-ling
YL
Zhou
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism*; D011655:Pulmonary Embolism / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D010959:Tissue Plasminogen Activator