Vascular associated death1, a novel GRAM domain-containing protein, is a regulator of cell death and defense responses in vascular tissues.
The hypersensitive response (HR) is a programmed cell death that is commonly associated with plant disease resistance. A novel lesion mimic mutant, vad1 (for vascular associated death1), that exhibits light conditional appearance of propagative HR-like lesions along the vascular system was identified. Lesion formation is associated with expression of defense genes, production of high levels of salicylic acid (SA), and increased resistance to virulent and avirulent strains of Pseudomonas syringae pv tomato. Analyses of the progeny from crosses between vad1 plants and either nahG transgenic plants, sid1, nonexpressor of PR1 (npr1), enhanced disease susceptibility1 (eds1), or non-race specific disease resistance1 (ndr1) mutants, revealed the vad1 cell death phenotype to be dependent on SA biosynthesis but NPR1 independent; in addition, both EDS1 and NDR1 are necessary for the proper timing and amplification of cell death as well as for increased resistance to Pseudomonas strains. VAD1 encodes a novel putative membrane-associated protein containing a GRAM domain, a lipid or protein binding signaling domain, and is expressed in response to pathogen infection at the vicinity of the hypersensitive lesions. VAD1 might thus represent a new potential function in cell death control associated with cells in the vicinity of vascular bundles.
Lorrain
Séverine
S
Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique 2594, Boîte Postale 27, 31326 Castanet-Tolosan, Cedex, France.
Lin
Baiqing
B
Auriac
Marie Christine
MC
Kroj
Thomas
T
Saindrenan
Patrick
P
Nicole
Michel
M
Balagué
Claudine
C
Roby
Dominique
D
D017209:Apoptosis / Q000502:physiology*; D017360:Arabidopsis / Q000033:anatomy & histology / Q000382:microbiology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000472:pathogenicity; D020156:Salicylic Acid / Q000378:metabolism; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D008565:Membrane Proteins; C109369:NDR1 protein, Arabidopsis; D011506:Proteins; D014157:Transcription Factors; D020156:Salicylic Acid