Effect of potassium channel modulators on toxicity of Cleistanthus collinus.
The study was conducted to determine the effects of boiled extract of Cleistanthus collinus on rats by observing ECG changes and electrolyte levels in serum and urine. Influence of minoxidil and glibenclamide on Cleistanthus collinus induced toxicity was determined. ED50 for arrhythmia, changes in contractility and heart rate were recorded using the isolated frog heart. Cleistanthus at low doses caused transient tachycardia and increase in contractility and at high dose caused arrhythmia and cardiac arrest in rat. LD50 was found to be 1690 mg/kg. Minoxidil potentiated cardiac toxicity, whereas glibenclamide did not produce any significant change. High concentration of potassium in Cleistanthus extract hindered comparison of its levels. There was excretion of sodium even in the presence of hyponatraemia. Cleistanthus at low dose caused transient tachycardia and increase in contractility and at high dose caused arrhythmia and cardiac arrest in isolated frog heart. ED50 for arrhythmia was found to be 1406 mg/kg. Acute toxicity was mainly due to depressive cardiac activity of Cleistanthus. It also caused renal failure. Potassium channel modulators did not have important role in acute cardiac toxicity treatment. Probably in chronic toxicity, electrolyte level changes are involved and potassium channel modulators might have a role.
Department of Pharmacology, Christian Medical College and Hospital, Vellore 632 002, India. vishnujose@hotmail.com
Vinu M
VM
Jose
K N
KN
Anand
L
L
Jeyaseelan
Kalpana
K
Ernest
Alice
A
Kuruvilla
D000818:Animals; D004562:Electrocardiography; D004573:Electrolytes / Q000378:metabolism; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D006027:Glycosides / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D017705:Lignans / Q000633:toxicity*; D008297:Male; D008914:Minoxidil / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D010936:Plant Extracts; D011188:Potassium / Q000737:chemistry / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry* / Q000378:metabolism; D011898:Ranidae; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000737:chemistry; D013997:Time Factors; D014197:Trees
D004573:Electrolytes; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; D015221:Potassium Channels; C010088:cleistanthin; D008914:Minoxidil; D012964:Sodium; D011188:Potassium; D005905:Glyburide