Lack of modulating influence of GSTM1 and GSTT1 polymorphisms on urinary biomonitoring markers in coke-oven workers.
BACKGROUND
Coke-oven workers (COWs) are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons (PAHs). Urinary 8-hydroxy-2-deoxyguanosine (8-OH-dG) and 1-hydroxypyrene (1-OHP) are biological markers of oxidative DNA damage and PAHs metabolism, respectively. In this study, we investigated whether polymorphisms of glutathione S-transferase (GSTM1 and GSTT1) can modulate the relationship between urinary 8-OH-dG and 1-OHP concentrations among the COWs.


METHODS
This was a cross-sectional study. Between February and November of 2001, 53 topside-oven and 130 side-oven workers with the presence of GSTM1 and GSTT1 genotypes were investigated.


RESULTS
Urinary 1-OHP and 8-OH-dG concentrations (mean +/- SD) in the topside-oven workers with the presence of GSTM1 were 107.2 +/- 107.9 and 15.3 +/- 9.7 ng/ml, respectively, which were not significantly different from those in the absence of GSTM1 (84.1 +/- 104.5 and 12.8 +/- 14.1 ng/ml). The similar insignificant results were also noted in the sideoven workers. For GSTT1 polymorphism, the results remained insignificant. In contrast, individual excretion of urinary 8-OH-dG and 1-OHP concentrations were still highly correlated (Spearman correlation coefficients: r = 0.43, P < 0.0001, n = 183).


CONCLUSIONS
GST may not play a role in the regulation of metabolism of urinary biological markers in COWs.
Graduate Institute of Occupational Safety and Health and Department of Occupational Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Ming-Tsang
MT
Wu
Chih-Hong
CH
Pan
Ching-Yi
CY
Chen
Chiou-Jong
CJ
Chen
Li-Hung
LH
Huang
Li-Yu
LY
Tsai
Chia-Tsuan
CT
Huang
Chi-Kung
CK
Ho
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000652:urine; D003430:Cross-Sectional Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000378:metabolism; D016273:Occupational Exposure; D016272:Occupational Health; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects / Q000378:metabolism*; D011110:Polymorphism, Genetic; D011721:Pyrenes / Q000378:metabolism*
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers; D003849:Deoxyguanosine; D005982:Glutathione Transferase; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C413545:glutathione S-transferase T1; C033146:1-hydroxypyrene