Terbium-sensitized fluorescence method for the determination of pazufloxacin mesilate and its application.
A simple, rapid and highly sensitive fluorometric method for the determination of pazufloxacin mesilate (PZFX) is described. It is based on the formation of the complex [Tb(PZFX)2](3+), which shows the intensive characteristic bands of Tb3+. Optimum conditions for the determination were investigated. Under the optimum experimental condition, the fluorescence intensity responds linearly to the PZFX concentration in the range of 2.0 x 10(-8) - 5.0 x 10(-6) mol/l with a detection limit of 6.2 x 10(-9) mol/l. The method has been successfully applied to the determination of PZFX in urine and serum samples.
Department of Chemistry, Beijing Normal University, Beijing, PR, China.
Shilv
S
Chen
Hongwu
H
Ma
Huichun
H
Zhao
Ruiqing
R
Feng
Linpei
L
Jin
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000493:pharmacokinetics*; D000995:Antitubercular Agents / Q000032:analysis; D002138:Calibration; D024841:Fluoroquinolones / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010078:Oxazines / Q000032:analysis* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000032:analysis*; D014556:Urine
D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D024841:Fluoroquinolones; D010078:Oxazines; D013725:Terbium; C078052:pazufloxacin