Accumulation of the common mitochondrial DNA deletion induced by ionizing radiation.
Point mutations and deletions in mitochondrial DNA (mtDNA) accumulate as a result of oxidative stress, including ionizing radiation. As a result, dysfunctional mitochondria suffer from a decline in oxidative phosphorylation and increased release of superoxides and other reactive oxygen species (ROS). Through this mechanism, mitochondria have been implicated in a host of degenerative diseases. Associated with this type of damage, and serving as a marker of total mtDNA mutations and deletions, the accumulation of a specific 4977-bp deletion, known as the common deletion (Delta-mtDNA(4977)), takes place. The Delta-mtDNA(4977) has been reported to increase with age and during the progression of mitochondrial degeneration. The purpose of this study was to investigate whether ionizing radiation induces the formation of the common deletion in a variety of human cell lines and to determine if it is associated with cellular radiosensitivity. Cell lines used included eight normal human skin fibroblast lines, a radiosensitive non-transformed and an SV40 transformed ataxia telangiectasia (AT) homozygous fibroblast line, a Kearns Sayre Syndrome (KSS) line known to contain mitochondrial deletions, and five human tumor lines. The Delta-mtDNA(4977) was assessed by polymerase chain reaction (PCR). Significant levels of Delta-mtDNA(4977) accumulated 72 h after irradiation doses of 2, 5, 10 or 20 Gy in all of the normal lines with lower response in tumor cell lines, but the absolute amounts of the induced deletion were variable. There was no consistent dose-response relationship. SV40 transformed and non-transformed AT cell lines both showed significant induction of the deletion. However, the five tumor cell lines showed only a modest induction of the deletion, including the one line that was deficient in DNA damage repair. No relationship was found between sensitivity to radiation-induced deletions and sensitivity to cell killing by radiation.
Department of Experimental Radiation Oncology, M.D. Anderson Cancer Center, The University of Texas, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Sheela
S
Prithivirajsingh
Michael D
MD
Story
Sherry A
SA
Bergh
Fady B
FB
Geara
K Kian
KK
Ang
Sheikh M
SM
Ismail
Craig W
CW
Stevens
Thomas A
TA
Buchholz
William A
WA
Brock
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000528:radiation effects*; D005260:Female; D005347:Fibroblasts / Q000502:physiology / Q000528:radiation effects*; D006801:Humans; D007259:Infrared Rays; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011839:Radiation, Ionizing; D017384:Sequence Deletion; D012867:Skin / Q000166:cytology
D017931:DNA Primers; D004272:DNA, Mitochondrial