Bone-constructing cells from ethmoid bone may have multilineage differentiation potential: preliminary report.
OBJECTIVE
In order to investigate multilineage differentiation in human cultured cells from ethmoid bone, we conducted a morphological study to examine adipogenic and chondrogenic differentiation.


METHODS
After reaching confluence, cells underwent terminal adipogenic differentiation by treatment with 100 microM indomethacin, 0.5 mM 1-methyl-3-isobutylxanthine, 1 microM dexamethasone (DEX), 10 microg/ml insulin and 0.3% dimethylsulfoxide in a medium supplemented with 10% fetal bovine serum. Chondrogenic differentiation was attempted by centrifuging a pelleted micromass using transforming growth factor-beta3 (TGF-beta3), DEX, ascorbic acid (AA), pyruvate acid, proline, glucose and (ITS)-plus.


RESULTS
The cultured cells displayed adipocyte but not chondrogenic lineage under these conditions. Considering the possibility that some differentiation potential may be lost with in vitro culture but maintained using another chondrogenic differentiation medium containing TGF-beta1, it is possible that cultured cells may have multilineage potential, including chondrogenic differentiation ability.


CONCLUSIONS
These morphological abilities of human cultured cells may indicate the possibility of the existence of mesenchymal stem cells in sinus bone. If mesenchymal stem cells exist in ethmoid bone, they may play an important role in future research on the regulation mechanisms of human bone tissue.
Ishino
Takashi
T
Department of Otorhinolaryngology, Head & Neck Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Japan. takashiishino@hotmail.com
Hirakawa
Katsuhiro
K
Takeno
Sachio
S
Furukido
Kyousuke
K
Sugimoto
Ichiro
I
Yajin
Koji
K
D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology; D024043:Collagen Type II / Q000032:analysis / Q000096:biosynthesis; D005004:Ethmoid Bone / Q000166:cytology* / Q000502:physiology; D006801:Humans; D008055:Lipids / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
D024043:Collagen Type II; D008055:Lipids