Comparative analysis of adenoviral transgene delivery via tail or portal vein into rat liver.
The potential indications for gene therapy are expanding continuously. Currently, hepatotropic adenoviruses are useful vector systems for targeting liver in experimental animal models. Although this gene delivery technique is widely distributed, there is no common sense about how these viruses should be applied. In general, the local delivery into portal vein and the systemic application via tail vein induces above all substantial transgene expression. We here comparatively analysed both methods and found that the systemic administration of an adeno-virus expressing the green fluorescent protein resulted in a stronger infiltration, a more homogenous distribution, and a higher inter-individual reproducibility of reporter gene expression in rat liver than organ-specific administration via the portal vein.
Herrmann
J
J
Institute of Clinical Chemistry and Pathobiochemistry, RWTH University Hospital, Aachen, Germany.
Abriss
B
B
van de Leur
E
E
Weiskirchen
S
S
Gressner
A M
AM
Weiskirchen
R
R
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D008099:Liver / Q000098:blood supply / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D011995:Recombination, Genetic; D013623:Tail / Q000098:blood supply; D019076:Transgenes
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins