Serum levels of matrix extracellular phosphoglycoprotein (MEPE) in normal humans correlate with serum phosphorus, parathyroid hormone and bone mineral density.
Matrix extracellular phosphoglycoprotein (MEPE), a member of the Small Integrin Binding Ligand N-linked Glycoprotein (SIBLING) family, is primarily expressed in normal bone and has been proposed as a phosphaturic factor because of high expression and secretion in oncogenic hypophosphatemic osteomalacia tumors. In order to begin to address the role of MEPE in normal human physiology, we developed a competitive ELISA to measure serum levels of MEPE. The ELISA was used to characterize the distribution pattern in a population consisting of 114 normal adult subjects. The mean value of MEPE was 476 +/- 247 ng/ml and levels decreased significantly with increasing age. MEPE levels were also significantly correlated with serum phosphorus and parathyroid hormone (PTH). In addition, MEPE levels correlated significantly with measures of bone mineral density in the femoral neck and total hip in a subset of 50 elderly subjects. The results are consistent with MEPE being involved in phosphate and bone metabolism in a normal population.
Division of Geriatric Medicine, Johns Hopkins Bayview Medical Center, 5501 Hopkins Bayview Circle, Room 5B 79 JHAAC, Baltimore, Maryland 21224, USA.
A
A
Jain
N S
NS
Fedarko
M T
MT
Collins
R
R
Gelman
M A
MA
Ankrom
M
M
Tayback
L W
LW
Fisher
D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood; D015519:Bone Density; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000097:blood*; D005272:Femur Neck / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood*; D006621:Hip Joint / Q000378:metabolism; D006801:Humans; D010281:Parathyroid Hormone / Q000097:blood*; D010750:Phosphoproteins / Q000097:blood*; D010758:Phosphorus / Q000097:blood*; D012016:Reference Values
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C486579:MEPE protein, human; D010281:Parathyroid Hormone; D010750:Phosphoproteins; D010758:Phosphorus