The O-antigen of Salmonella enterica serotype Enteritidis PT4: a significant factor in gastrointestinal colonisation of young but not newly hatched chicks.
The lipopolysaccharide of Salmonella and other Gram negative pathogenic species has been implicated as a major virulence determinant and in this study we report the role of LPS of S. Enteritidis in the colonisation and persistent gastrointestinal infection of young poultry. The gene encoding the unique O-antigen ligase, waaL, was mutated by insertional inactivation in a well characterised S. Enteritidis strain, S1400/94. The waaL mutant, designated PCP, produced rough colonies on agar medium, did not agglutinate O9 antiserum, did not produce an LPS ladder on silver stained gels and was serum sensitive. PCP and a nalidixic acid marked derivative of S1400/94 (S1400/94 Nalr) were used to orally challenge young chicks, separately and together in competitive index experiments. At post-mortem examination of 1-day-old chicks challenged S1400/94 Nalr and PCP separately there were no significant differences in the numbers of S1400/94 Nalr and PCP bacteria in tissues sampled on days 1, 2, and 5. By day 42 after challenge S1400/94 Nalr bacteria were recovered in significantly higher numbers than PCP from the caecal contents (P < 0.001). In competitive index studies in the 1-day-old chick PCP colonised, invaded and persisted in lower numbers than S1400/94 Nalr. In 4-week-old chicks challenged separately, PCP bacteria were recovered from all tissues examined in significantly lower numbers than S1400/94 Nalr. In competitive index experiments in 4-week-old chicks, PCP was not detected at any site and at any time point. Therefore, the O-antigen of S. Enteritidis plays an important role in poultry infections although this role is less important in the newly hatched chick.
Department of Food and Environmental Safety, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
P
P
Carroll
R M
RM
La Ragione
A R
AR
Sayers
M J
MJ
Woodward
D000367:Age Factors; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000662:veterinary; D004064:Digestive System / Q000276:immunology / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000276:immunology; D016254:Mutagenesis, Insertional; D019081:O Antigens / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology*; D011897:Random Allocation; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D013047:Specific Pathogen-Free Organisms; D018709:Statistics, Nonparametric
D008070:Lipopolysaccharides; D019081:O Antigens