Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities.
Chemical and Biological Engineering Department, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Grazyna E
GE
Sroga
Jonathan S
JS
Dordick
D002477:Cells / Q000201:enzymology*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D003470:Culture Media; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D015202:Protein Engineering / Q000379:methods*; D013381:Subtilisins / Q000235:genetics
D003470:Culture Media; D004798:Enzymes; D013381:Subtilisins