Low expression of the putative tumour suppressor gene gravin in chronic myeloid leukaemia, myelodysplastic syndromes and acute myeloid leukaemia.
The putative tumour suppressor gene gravin is down-regulated in several solid tumours and is implicated in tumorigenesis. We have evaluated the expression levels of the gravin gene in the CD34(+)/blast cells of a range of myeloid malignancies as compared with controls using real-time quantitative polymerase chain reaction (PCR). Gravin was markedly down-regulated in 41 of 41 patients with acute myeloid leukaemia (AML), nine of 10 patients with myelodysplastic syndromes (MDS) and 33 of 33 patients with chronic myeloid leukaemia (CML), of whom 24 were in blast crisis (BC). We have shown that gravin is consistently down-regulated in the CD34(+)/blast cells of myeloid malignancies and may play a role in the molecular pathogenesis of these disorders.
Leukaemia Research Fund Molecular Haematology Unit, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, UK. jacqueline.boultwood@ndcls.ox.ac.uk
Jacqueline
J
Boultwood
Andrea
A
Pellagatti
Fiona
F
Watkins
Lisa J
LJ
Campbell
Noor
N
Esoof
Nicholas C P
NC
Cross
Helen
H
Eagleton
Tim J
TJ
Littlewood
Carrie
C
Fidler
James S
JS
Wainscoat
D054758:A Kinase Anchor Proteins; D000208:Acute Disease; D018952:Antigens, CD34 / Q000032:analysis; D001752:Blast Crisis / Q000235:genetics; D018797:Cell Cycle Proteins; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004252:DNA Mutational Analysis; D015536:Down-Regulation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology; D007951:Leukemia, Myeloid / Q000235:genetics*; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054758:A Kinase Anchor Proteins; C491162:AKAP12 protein, human; D018952:Antigens, CD34; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; D011506:Proteins