[Profiles of the expression of phospholipase A2 family mRNAs by macrophages infected with Mycobacterium tuberculosis].
Previously, we found that collaboration of reactive nitrogen intermediates (RNI) and free fatty acids (FFA) plays crucial roles in the expression of macrophage (Mphi) antimicrobial activity against Mycobacterium tuberculosis (MTB). Phospholipase A2 (PLA2) families hydrolyze phospholipids and release FFA from their sn-2 position. In the present study, we examined profiles of the mRNA expression of PLA2 families by Mphis stimulated with MTB by using RT-PCR method, and the following results were obtained. First, the expression of type IV cytosolic PLA2 (cPLA2), which is highly specific to arachidnic acid moiety, was significantly up-regulated by MTB stimulation of Mphis. Second, the expression of type IIa secretory PLA2 (sPLA2) was not observed for Mphis with or without MTB stimulation. Third, the profile of mRNA expression of type V sPLA2 was nearly the same as that of type IV cPLA2 for Mphis before and after MTB stimulation. These findings suggest that type IV cPLA2 and type V sPLA2 both play important roles in the FFA-mediated Mphi Cap antimicrobial activity against MTB organisms.
Sano
Chiaki
C
Department of Microbiology and Immunology, Shimane University School of Medicine.
Shimizu
Toshiaki
T
Sato
Katsumasa
K
Tomioka
Haruaki
H
D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000502:physiology; D008264:Macrophages / Q000201:enzymology* / Q000382:microbiology*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D010741:Phospholipases A / Q000096:biosynthesis* / Q000235:genetics; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000096:biosynthesis; D026361:Reactive Nitrogen Species / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; D026361:Reactive Nitrogen Species; D010741:Phospholipases A; D054467:Phospholipases A2