Chitinibacter tainanensis gen. nov., sp. nov., a chitin-degrading aerobe from soil in Taiwan.
Five strains with strong chitinolytic activity were isolated from a soil sample collected from southern Taiwan. The strains shared more than 92% DNA-DNA similarity, indicating membership of the same genospecies. This close relationship was supported by high similarities in fatty acid composition and biochemical characteristics. A 16S rRNA gene sequence analysis indicated that the isolates were members of the class 'Betaproteobacteria', in which they formed an individual subline of descent that was distantly related (<94% similarity) to lineages defined by Formivibrio citricus DSM 6150T and Iodobacter fluviatilis DSM 3764T. On the basis of the phylogenetic and phenotypic distinctness of these novel chitin-degrading organisms, a new genus, Chitinibacter, is proposed, with Chitinibacter tainanensis (type strain, S1T=BCRC 17254T=DSM 15459T) as the type species.
Chern
Lih-Ling
LL
Bioresource Collection and Research Centre, Food Industry Research and Development Institute, PO Box 246, Hsinchu 300, Taiwan.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zelkuturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Lee
Shu-Fen
SF
Bioresource Collection and Research Centre, Food Industry Research and Development Institute, PO Box 246, Hsinchu 300, Taiwan.
Lee
Fwu-Ling
FL
Bioresource Collection and Research Centre, Food Industry Research and Development Institute, PO Box 246, Hsinchu 300, Taiwan.
Chen
Jeen-Kuan
JK
Department of Environment and Biotechnology, Refining and Manufacturing Research Centre, Chinese Petroleum Corporation, Chia-Yi, Taiwan.
Fu
Huei-Mei
HM
Bioresource Collection and Research Centre, Food Industry Research and Development Institute, PO Box 246, Hsinchu 300, Taiwan.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002686:Chitin / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000648:ultrastructure; D020459:Genes, rRNA; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D013624:Taiwan; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002686:Chitin