Copper deficiency induced expression of Fe-superoxide dismutase gene in Matteuccia struthiopteris.
Iron-superoxide dismutase (Fe-SOD) activity was not detected in extracts from the leaves of ferns, Equisetum arvense and Matteuccia struthiopteris. To know why ferns lack Fe-SOD activity, the Fe-SOD like gene (MsFeSOD1) was isolated from M. struthiopteris and its expression was investigated with a focus on the metals Fe and Cu using the prothalli of the fern. The expression of MsFeSOD1 mRNA was induced by a deficiency of Cu, but Fe-SOD activity was not detected. The recombinant protein of MsFeSOD1 produced in E. coli showed Fe-SOD activity. These findings suggest that the fern Fe-SOD like gene was transcriptionally regulated by Cu but an additional mechanism is involved in the formation of an active enzyme.
Murao
Kenichi
K
Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan.
Takamiya
Masayuki
M
Ono
Kanji
K
Takano
Hiroyoshi
H
Takio
Susumu
S
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D003300:Copper / Q000172:deficiency / Q000494:pharmacology / Q000502:physiology*; D029624:Ferns / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics*
D011994:Recombinant Proteins; D003300:Copper; D013482:Superoxide Dismutase