SERF: a new antigen in the Cromer blood group system.
The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021.012 (CROM 12).
Banks
J
J
International Blood Group Reference Laboratory, Bristol, UK.
Poole
J
J
Ahrens
N
N
Seltsam
A
A
Salama
A
A
Hue-Roye
K
K
Storry
J R
JR
Palacajornsuk
P
P
Ma
B-W
BW
Lublin
D M
DM
Reid
M E
ME
D001483:Base Sequence; D001789:Blood Group Antigens / Q000032:analysis*; D017931:DNA Primers; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001789:Blood Group Antigens; D017931:DNA Primers; D007519:Isoantigens; C499797:SERF antigen, human