The effect of captivity and diet on KLH isoform ratios in Megathura crenulata.
Aquaculture of the giant keyhole limpet, Megathura crenulata, may provide a reliable long-term supply of keyhole limpet hemocyanin (KLH) for many promising biomedical applications. However, previous studies have reported a complete loss of the KLH1 isoform under certain cultivation conditions. We examined whether captivity per se and diet caused a significant change in the isoform profile of M. crenulata. Although there was a trend toward a decreasing percentage of KLH1 in some animals, in general isoform profiles were not significantly affected by captivity or dietary limitations. Further, the percentage of KLH1 significantly increased for limpets with previously low levels of KLH1 when fed a supplemental mixed diet. Our results indicate that normal isoform profiles can be maintained in limpets held in captivity even when fed insufficient diets, and that these conditions do not cause a complete loss of either KLH isoform. Notably, the enhancement of abnormally low levels of KLH1 suggests that variability in isoform profiles could potentially be minimized through diet. While there is a need for further research on the factors responsible for the variability of KLH, overall, these results support the premise that culture of M. crenulata may provide a sustainable source of this biomedically important product.
Stellar Biotechnologies, Aquaculture Division, Inc., 417 E. Hueneme Road #170, Port Hueneme, CA 93041, USA. LiveOaks@aol.com
Frank R
FR
Oakes
Sarah
S
McTee
John
J
McMullen
Carolynn S
CS
Culver
Daniel E
DE
Morse
D000818:Animals; D004032:Diet; D006433:Hemocyanins / Q000737:chemistry* / Q000378:metabolism*; D008974:Mollusca / Q000737:chemistry*; D020033:Protein Isoforms; D013045:Species Specificity
D020033:Protein Isoforms; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin