Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.
Cytokinins are hormones that are involved in plant growth and development. They are irreversibly degraded by cytokinin oxidases/dehydrogenases, flavoenzymes which contain a covalently bound flavine adenine dinucleotide (FAD) cofactor. Cytokinin oxidase from Zea mays (ZmCKO1) was overexpressed in the yeast Yarrowia lipolytica, purified (molecular weight 69 kDa) and crystallized using the hanging-drop method. Crystals belong to the monoclinic space group C2, with unit-cell parameters a = 250.6, b = 50.6, c = 51.5 A, beta = 94.1 degrees. A complete data set has been collected at 100 K to 1.95 A resolution on an X-ray synchrotron source.
Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr, 78026 Versailles CEDEX, France.
David
D
Kopecný
Pierre
P
Briozzo
Nathalie
N
Joly
Nicole
N
Houba-Hérin
Catherine
C
Madzak
Claude
C
Pethe
Michel
M
Laloue
D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D010088:Oxidoreductases; C019984:cytokinin oxidase