Isolation and some properties of fimbriae of oral Streptococcus intermedius.
Streptococcus intermedius 1208-1 carried linear fiber-like fimbriae that extended radially from the cell surface. The fimbriae were isolated by pipetting and sonication and were purified by ammonium sulfate precipitation followed by a column chromatography series. Heat treatment in the presence of sodium dodecyl sulfate resulted in the dissociation into smaller molecules. Rabbit antiserum raised against the purified protein reacted with fimbriae on the surface of bacteria under immunogold staining. Serotype g or g-related strains produced the fimbriae and aggregated in human saliva. The aggregation was inhibited by the anti-fimbriae immunoglobulin Fab fragment or the purified fimbriae.
Department of Oral Medicine and Health Promotion, Kagoshima University Dental School, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan. yamatai@dentb.hal.kagoshima-u.ac.jp
Taihei
T
Yamaguchi
Nobuko
N
Matsunoshita
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D036781:Fimbriae Proteins / Q000302:isolation & purification*; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000648:ultrastructure; D034367:Streptococcus intermedius / Q000737:chemistry* / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D036781:Fimbriae Proteins