Influences of excessive Cu on photosynthesis and growth in ectomycorrhizal Pinus sylvestris seedlings.
Growth and photosynthesis responses were measured for Scots pine (Pinus sylvestris L. cv.) inoculated with ectomycorrhizal fungi (Suillus bovinus) under 6.5 and 25 mg/L Cu treatments to evaluate ectomycorrhizal seedlings' tolerance to heavy metal stress. Results showed that excessive Cu can significantly impair the growth and photosynthesis of pine seedlings, but such impairment is much smaller to the ectomycorrhizal seedlings. Under 25 mg/L Cu treatment, the dry weight of ectomycorrhizal seedlings is 25% lower than the control in contrary to 53% of the non-mycorrhizal seedlings, and the fresh weight of ectomycorrhizal roots was significantly higher than those of non-mycorrhizal roots, about 25% and 42% higher at 6.5 and 25 mg/L Cu treatments respectively. Furthermore, ectomycorrhizal fungi induced remarkable difference in the growth rate and pigment content of seedlings under excessive Cu stress. At 25 mg/L Cu, the contents of total chlorophyll, chlorophyll-a and chlorophyll-b were 30% higher in ectomycorrhizal plants than those in non-mycorrhizal plants. O2 evolution and electron transport of PSI and PSII were restrained by elevated Cu stress. However, no significant improvement was observed in reducing the physiological restraining in ectomycorrhizal seedlings over the non-mycorrhizal ones.
College of Environmental Sciences, Peking University, Beijing 100871, China. yhuang@urban.pku.edu.cn
Yi
Y
Huang
Shu
S
Tao
D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D003300:Copper / Q000633:toxicity*; D038821:Mycorrhizae / Q000254:growth & development*; D010788:Photosynthesis; D028223:Pinus / Q000382:microbiology* / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D036226:Seedlings; D012989:Soil Pollutants / Q000633:toxicity*
D012989:Soil Pollutants; D002734:Chlorophyll; C037184:chlorophyll b; D003300:Copper; D000077194:Chlorophyll A