Transesterification activity of lipases immobilized in a phyllosilicate sol-gel matrix.
Lipases from Pseudomonas cepacia (P.c.) and Thermomyces lanuginosa (T.l.) were immobilized in a phyllosilicate sol-gel matrix and studied for their ability to catalyze the alcoholysis of fats and oils to simple alkyl esters. At 50 degrees C and 48 h reaction immobilized T.l. lipase gave higher alkyl ester yields (70 to 100%) from fats and oils regardless of chain length or degree of unsaturation of the acyl groups in the triacylglycerols than did immobilized P.c. lipase (20-90%), which preferred unsaturated oils. Both immobilized lipases catalyzed ester formation (80-90%) from greases containing a range of free fatty acids (2.6 to 36%). Molecular sieves had no effect on ester yields in the immobilized T.l. lipase-catalyzed alcoholysis of greases but did improve yields (5-10%) in the immobilized P.c. lipase-catalyzed reactions.
Hsu
An-Fei
AF
US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. ahsu@errc.ars.usda.gov
Jones
Kerby C
KC
Foglia
Thomas A
TA
Marmer
William N
WN
D016956:Burkholderia cepacia / Q000201:enzymology*; D002384:Catalysis; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D000431:Ethanol; D005223:Fats / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008567:Membranes, Artificial; D009821:Oils / Q000737:chemistry*; D044367:Phase Transition; D017640:Silicates / Q000737:chemistry*; D013149:Spiroplasma / Q000201:enzymology*
D004800:Enzymes, Immobilized; D005223:Fats; D008567:Membranes, Artificial; D009821:Oils; D017640:Silicates; D000431:Ethanol; D008049:Lipase