The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae.
Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H(+)-ATPase and the plasma membrane H(+)-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H(+)-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.
Kellermayer
Richard
R
Department of Medical Genetics and Child Development Medicine, University of Pécs, Szigeti ut 12, 7623 Pécs, Hungary. richard.kellermayer@aok.pte.hu
Szigeti
Réka
R
Kellermayer
Miklós
M
Miseta
Attila
A
D002118:Calcium / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005838:Genotype; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D006180:Proton-Translocating ATPases; D005947:Glucose; D002118:Calcium