Oocyte transfer and gamete intrafallopian transfer in the mare.
Methods for the collection and transfer of equine oocytes have been developed, and uses of these techniques have resulted in new clinical and research possibilities. Because oocyte transfer avoids reproductive problems associated with the oviduct, uterus, and cervix, pregnancies can be produced from many mares that cannot carry a pregnancy or produce embryos. Oocytes for clinical transfers are usually collected from preovulatory follicles and cultured for a short interval or transferred directly into a recipient's oviduct. For oocyte transfer, the recipient is inseminated within the uterus. A large number (1 x 10(9) to 2 x 10(9)) of motile sperms are preferred for inseminations. In contrast, sperm and oocyte are transferred into the oviduct during gamete intrafallopian transfer (GIFT). Therefore, a lower number (1 x 10(5) to 2 x 10(5)) of sperm can be used. Potentially, GIFT could be used in situations where sperm numbers are limited. Use of oocyte transfer and GIFT in clinical and research settings will aid us in understanding the interactions between oocyte, sperm, and oviduct in the equine.
Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. emc@colostate.edu
E M
EM
Carnevale
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D015181:Gamete Intrafallopian Transfer / Q000662:veterinary*; D006736:Horses; D009865:Oocytes / Q000637:transplantation*; D011247:Pregnancy; D020858:Tissue and Organ Harvesting / Q000662:veterinary