[Esterase activities of Candida species].
The aim of this study was to determine the esterase activity of clinically important Candida species and to evaluate tween 80 opacity test medium as an additional identification method. A total of 118 Candida isolates (86 C. albicans, 12 C. tropicalis, 5 C. glabrata, 4 C. krusei, 3 C. guilliermondii, 3 C. kefyr, 3 C. parapsilosis, 1 C. famata, 1 C. dubliniensis) were examined for their response to tween 80 opacity test. Our results showed that, 83 of 86 (96.5%) Candida albicans, and all of the C. tropicalis and C. guilliermondii strains (100%) yielded a halo around their colonies. The remaining Candida species did not produce a positive test response after 13 days of incubation. It can be concluded that, tween 80 opacity test is a simple and economical test that can be used as a supplementary test for the identification of Candida species.
Kocaeli Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Kocaeli.
Sema Aşkin
SA
Keçeli
Fatma
F
Budak
D002175:Candida / Q000201:enzymology* / Q000302:isolation & purification; D003470:Culture Media; D004950:Esterases / Q000378:metabolism*; D006801:Humans; D011136:Polysorbates; D013501:Surface-Active Agents
D003470:Culture Media; D011136:Polysorbates; D013501:Surface-Active Agents; D004950:Esterases