Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in cultured tobacco cells.
Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent dyes and green fluorescent protein (GFP). Observation using the endocytosis markers, FM4-64 and Lucifer Yellow CH, suggested that there is a membrane flow from the plasma membrane to autolysosomes. Using these dyes as well as GFP-AtVam3p, sporamin-GFP and gamma-VM23-GFP fusion proteins as markers of the central vacuole, we found transport of components of the central vacuole to autolysosomes. Thus endocytosis and the supply from the central vacuole may contribute to the formation of autolysosomes.
Yano
Kanako
K
School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan.
Matsui
Sumiko
S
Tsuchiya
Tomohiro
T
Maeshima
Masayoshi
M
Kutsuna
Natsumaro
N
Hasezawa
Seiichiro
S
Moriyasu
Yuji
Y
D001343:Autophagy / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D004705:Endocytosis / Q000502:physiology; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism* / Q000648:ultrastructure
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins