Application of derivative spectrophotometry for simultaneous determination of quinapril and hydrochlorothiazide in the combination tablets.
A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI ). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 microg/mL for QUI and 2.5 to 15.0 microg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 microg/mL for QUI and 0.12 and 0.4 microg/mL for HYD, respectively. The accuracy (recovery 100.5-102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.
Medical University of Lublin, Department of Medicinal Chemistry, Faculty of Pharmacy, Chodźki Str, Lublin, Poland. kzchl@asklepios.am.lublin.pl
Dorota
D
Kowalczuk
Hanna
H
Hopkala
D000959:Antihypertensive Agents / Q000032:analysis*; D002138:Calibration; D004338:Drug Combinations; D004355:Drug Stability; D006852:Hydrochlorothiazide / Q000032:analysis*; D000432:Methanol; D000077583:Quinapril; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D044005:Tetrahydroisoquinolines / Q000032:analysis*
D000959:Antihypertensive Agents; D004338:Drug Combinations; D012997:Solvents; D013607:Tablets; D044005:Tetrahydroisoquinolines; D006852:Hydrochlorothiazide; D000077583:Quinapril; D000432:Methanol