Solid-state fermentation for production of griseofulvin on rice bran using Penicillium griseofulvum.
Griseofulvin is a secondary metabolite produced from fungal species that have morphology suitable for solid-state fermentation (SSF). Reports on production of griseofulvin by SSF are scarce. The present work investigates SSF for griseofulvin production, optimization of its process parameters vis-à-vis the conventional submerged fermentation and its downstream processing from the same. Rice bran adjusted to an initial moisture content (IMC) of 50% (v/w) inoculated with 1 mL of a suspension of 10(6) spores/mL under agitation at 250 rpm containing the modified Czapek-Dox medium and additional 0.1% choline chloride as a precursor gave a yield of griseofulvin in 9 days that was comparable to submerged fermentation after 28 days. The yield of griseofulvin (microg/g dry biomass) was comparable in SSF and submerged fermentation. The biomass was estimated by estimation of chitin. Discussions on the effect of each parameter in SSF have also been included.
Food and Fermentation Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
Sayali S
SS
Saykhedkar
Rekha S
RS
Singhal
D002855:Chromatography, Thin Layer / Q000379:methods; D005285:Fermentation; D006118:Griseofulvin / Q000096:biosynthesis*; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D010407:Penicillium / Q000378:metabolism*; D013696:Temperature
D006118:Griseofulvin