[Screening of cDNA library of Schistosoma japonicum with sera from rabbits vaccinated with ultraviolet-attenuated schistosomula].
OBJECTIVE
To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae.


METHODS
Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced.


RESULTS
Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.


CONCLUSIONS
Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Xiao-hong
XH
Li
Shu-xian
SX
Liu
Guang-cheng
GC
Song
Yu-xin
YX
Xu
Jian-ping
JP
Cao
Jia-xu
JX
Chen
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017238:Genes, Helminth; D007106:Immune Sera / Q000276:immunology*; D007814:Larva / Q000276:immunology / Q000528:radiation effects; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology*; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology
D018076:DNA, Complementary; D007106:Immune Sera; D014613:Vaccines, Attenuated