Improved cell growth and Taxol production of suspension-cultured Taxus chinensis var. mairei in alternating and direct current magnetic fields.
The growth of suspension cultures of Taxus chinensis var. mairei and Taxol production were promoted both by a sinusoidal alternating current magnetic field (50 Hz, 3.5 mT) and by a direct current magnetic field (3.5 mT). Taxol production increased rapidly from the 4th d with the direct current magnetic field but most slowly with the alternating current magnetic field. The maximal yield of Taxol was 490 microg l(-1) with the direct current magnetic field and 425 microg l(-1) with the alternating current magnetic field after 8 d of culture, which were, respectively, 1.4-fold and 1.2-fold of that without exposure to a magnetic field.
Department of Pharmaceutical Engineering, School of Chemical Engineering & Technology, Tianjin University, 300072, PR China.
Gui-Min
GM
Shang
Jin-Chuan
JC
Wu
Ying-Jin
YJ
Yuan
D000222:Adaptation, Physiological / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D017239:Paclitaxel / Q000096:biosynthesis*; D020946:Taxus / Q000502:physiology* / Q000528:radiation effects*
D017239:Paclitaxel