In vitro conidial germination in Arthrinium aureum and Arthrinium phaeospermum.
This paper describes the microscopic details of conidial germination and the influence of pH, sodium chloride concentration, 3% glucose, 3% saccharose and ultraviolet irradiation on the conidial germination in Arthrinium species. Under laboratory conditions, germination started after an incubation period of 90 minutes in 2% malt extract broth at 25 degrees C. In vitro, the conidia of Arthrinium species have a very low percentage of germination (A. phaeospermum: 7.9%; A. aureum: 15.8%). Conidia of this genus have a characteristic equatorial slit. Conidia may break spontaneously at this slit, releasing their cytoplasmic contents. Arthrinium phaeospermum attains its optimum germination percentage when its conidia are suspended in a sterile saline solution (pH 3.5) in a water bath at 20 degrees C for 15 minutes before being inoculated on 2% malt extract agar. Conidial suspensions of A. aureum may be held in the same conditions, but for 30 minutes.
Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain. magut@iqs.edu
Montserrat
M
Agut
María Angeles
MA
Calvo
D001203:Ascomycota / Q000254:growth & development* / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012965:Sodium Chloride / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014466:Ultraviolet Rays
D012965:Sodium Chloride; D013395:Sucrose; D005947:Glucose