Zona-float method for separating mouse eggs from other cells.
We have developed a new method for separating mouse eggs from other cells, such as cumulus cells, using centrifugation with Percoll. Solutions of 45, 22.5, 11.3, and 5.6% Percoll were tested. With the 22.5% solution, 99% of whole eggs obtained by in vitro fertilization were collected from the upper part of the Percoll solution, and 98% of 2-cell embryos collected from these eggs developed to the blastocyst stage. Offspring were obtained after transfer of collected embryos to female mice. The greatest advantage of this method is that undamaged eggs are separated from other cells in one simple operation, regardless of the number of eggs.
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Tsukuba, Ibaraki, Japan.
Hironori
H
Okada
Yoshihiro
Y
Hirose
Periyasamy
P
Manonmani
Masao
M
Ito
Tadashi
T
Sankai
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D008297:Male; D051379:Mice; D010063:Ovum; D011205:Povidone; D012822:Silicon Dioxide
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone