Simple and viable in vitro perfusion model for training microvascular anastomoses.
In this report, we describe a novel in vitro perfused microvessel model for training microvascular anastomotic exercises. Arteries and veins with a diameter of ca. 1 mm were explanted from chicken wings. These vessels were cannulated at both ends and mounted on a platform. Preserved, expired whole blood obtained from the blood bank was continuously injected through the proximal catheter, using an automatic perfusor. This in vitro perfused microvessel model exactly simulated the viable small-animal vessels. The setting is very simply and reliably repeated; the materials used are very cheap and universally available. There are no ethical questions involved. Vessels explanted from the human placenta or omentum may be used in a similar manner to gain the "feel" of functioning human microvascular tissue. But such materials are rarer and require the approval of ethical committees.
Krishnan
Kartik G
KG
Department of Neurological Surgery, Carl Gustav Carus University Hospital, Technical University of Dresden, Dresden, Germany. Kartik.Krishnan@uniklinikum-dresden.de
Dramm
Peter
P
Schackert
Gabriele
G
D000714:Anastomosis, Surgical / Q000193:education; D000818:Animals; D002645:Chickens; D066298:In Vitro Techniques; D008866:Microsurgery / Q000193:education*; D010477:Perfusion / Q000379:methods; D013663:Teaching / Q000379:methods*; D014656:Vascular Surgical Procedures / Q000193:education*; D014921:Wings, Animal / Q000098:blood supply*