Sea-buckthorn juice protects mice against genotoxic action of cisplatin.
OBJECTIVE
To study the influence of sea-buckthorn (SB) juice on the micronucleus (MN) frequency in bone marrow cells and sperm abnormality induced by cisplatin (CP, cis-dichlordiammineplatinum-II).


METHODS
The experiments were performed with male Swiss albino mice. SB juice (0.3 ml) prepared ex tempore was given to mice by gavage during 5 or 10 days. 3 h after the last gavage, mice received CP at doses either 1.2 mg/kg or 2.4 mg/kg i.p. MN frequency was studied in bone marrow polychromatic erythrocytes 24 h after the injection of the drug. The abnormality of sperm heads was studied by microscopy.


RESULTS
The SB juice decreased significantly the number of MN in bone marrow cells induced by CP at dose of 1.2 mg/kg by 36.5% and 47.9% (when it was given 5 and 10 days, consequently), and by 19.0% (p > 0.05) at dose of 2.4 mg/kg. The SB juice decreased significantly also the damaging effect of CP on sperm head at low dose and not significantly at the higher one. Antigenotoxic effect of juice was 45.0% and 16.6%, respectively.


CONCLUSIONS
SB juice decreased significantly the genotoxic effect of CP at dose of 1.2 mg/kg on somatic (bone marrow) and germ (sperm) cells of mice. At higher dose of the drug the effects was not statistically significant.
Cancer Research Centre, Yerevan, Armenia. armenn@freenet.am
Armen
A
Nersesyan
Rafael
R
Muradyan
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002945:Cisplatin / Q000633:toxicity*; D005638:Fruit; D031281:Hippophae; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology
D000970:Antineoplastic Agents; D002945:Cisplatin