Effect of pH on the production of the antitumor antibiotic retamycin by Streptomyces olindensis.
The effect of pH on cell growth and retamycin production in batch bioreactor cultures of Streptomyces olindensis ICB20 was investigated. In fermentations pH-controlled over the range 6.0-8.0, the highest retamycin production was achieved at pH 7.0, and the maximum concentration of retamycin, about 1.36 A (absorbance) units, was about 43, 58 and 232% higher than the values obtained at pH 7.5, 6.0 and 8.0 respectively.
Departamento de Engenharia Química, Escola Politécnica da Universidade de São Paulo, P.O. Box 61548, CEP 05424-970, São Paulo, SP, Brazil.
Luciana M
LM
Guimarães
Renata L de Araújo
RL
Furlan
Leandro M
LM
Garrido
Armando
A
Ventura
Gabriel
G
Padilla
Maria Cândida R
MC
Facciotti
D018943:Anthracyclines / Q000378:metabolism*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D006863:Hydrogen-Ion Concentration; D013302:Streptomyces / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; C002920:retamycin