[Effects of nandrolone phenylpropionate on fibroblasts after injury in rats].
OBJECTIVE
To explore the effects of nandrolone phenylpropionate (NP) on fibroblasts after injury in rats.


METHODS
Thirty-two Wistar rats with a deep second-degree scald injury and 20% total body surface area were randomly divided into two groups to receive either 5 mg/kg NP (NP group) or normal saline as placebo (control group) every other day. Integrated optical density (IOD) of androgen receptor (AR) and mRNA expression level of alpha1 (I) procollagen were measured by immunohistochemistry and quantitive fluorescent RT-PCR individually on the post-burn days 4, 7, 14 and 21. Fibroblasts were isolated from granulation tissue of a Wistar rat and were cultured in RPMI1640 with the addition of NP at different concentrations. Cell viability of fibroblasts was measured by MTT test, and the proliferative index, by flow cytometry (FCM).


RESULTS
(1) Compared with the A value of the concurrent control group, higher A values were seen in NP groups containing different concentrations of NP, and significant difference of proliferative index was observed in NP group, P<0.05. (2) The expression of alpha1 (I) procollagen mRNA in NP groups was much higher than that in control groups. A significant difference between the two groups was noted on the post-burn days 7, 14 and 21, P<0.05, but no difference was seen on day 4. (3) The density of AR on fibroblasts in NP group was higher than that in control group at each time point. And a positive correlation between the expression of alpha1 (I) procollagen mRNA and the quantity of AR on fibroblasats was confirmed (r = 0.836).


CONCLUSIONS
Nandrolone phenylpropionate could promote fibroblast replication, increase the mRNA level of alpha1 (I) procollagen and enhance the density of AR on fibroblasts.
Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Ying
Y
Cen
Ning
N
Liu
Xiao-xue
XX
Liu
Kai
K
Li
D045930:Anabolic Agents / Q000494:pharmacology; D000818:Animals; D002056:Burns / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D006097:Granulation Tissue / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009277:Nandrolone / Q000494:pharmacology*; D010666:Phenylpropionates / Q000494:pharmacology; D011347:Procollagen / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism
D045930:Anabolic Agents; D010666:Phenylpropionates; D011347:Procollagen; D012333:RNA, Messenger; D011944:Receptors, Androgen; D009277:Nandrolone