Islet allograft survival induced by costimulation blockade in NOD mice is controlled by allelic variants of Idd3.
NOD mice develop type 1 autoimmune diabetes and exhibit genetically dominant resistance to transplantation tolerance induction. These two phenotypes are genetically separable. Costimulation blockade fails to prolong skin allograft survival in (NOD x C57BL/6)F1 mice and in NOD-related strains made diabetes-resistant by congenic introduction of protective major histocompatibility complex (MHC) or non-MHC Idd region genes. Here, we tested the hypothesis that the genetic basis for the resistance of NOD mice to skin allograft tolerance also applies to islet allografts. Surprisingly, costimulation blockade induced permanent islet allograft survival in (NOD x C57BL/6)F1 mice but not in NOD mice. After costimulation blockade, islet allograft survival was prolonged in diabetes-resistant NOD.B6 Idd3 mice and shortened in diabetes-free C57BL/6 mice congenic for the NOD Idd3 variant. Islet allograft tolerance could not be induced in diabetes-resistant NOD.B10 Idd5 and NOD.B10 Idd9 mice. The data demonstrate that 1) NOD mice resist islet allograft tolerance induction; 2) unlike skin allografts, resistance to islet allograft tolerance is a genetically recessive trait; 3) an Idd3 region gene(s) is an important determinant of islet allograft tolerance induction; and 4) there may be overlap in the mechanism by which the Idd3 resistance locus improves self-tolerance and the induction of allotolerance.
The University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Todd
T
Pearson
Peter
P
Weiser
Thomas G
TG
Markees
David V
DV
Serreze
Linda S
LS
Wicker
Laurence B
LB
Peterson
Anne-Marie
AM
Cumisky
Leonard D
LD
Shultz
John P
JP
Mordes
Aldo A
AA
Rossini
Dale L
DL
Greiner
D000483:Alleles; D000818:Animals; D023201:CD40 Ligand / Q000276:immunology; D003131:Combined Modality Therapy; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000601:surgery; D014644:Genetic Variation; D006085:Graft Survival / Q000502:physiology*; D007167:Immunotherapy; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D014184:Transplantation, Homologous
D023201:CD40 Ligand