Duffy antigen is important for the lethal effect of the lethal strain of Plasmodium yoelii 17XL.
We studied the potential role of the Duffy antigen and glycophorin A as receptors for rodent malaria parasite invasion of erythrocytes. Parasitemia increased exponentially after infection with Plasmodium berghei NK65, P. chabaudi, and P. vinckei in Duffy antigen knockout, glycophorin A knockout, and wild-type mice, indicating that the Duffy antigen and glycophorin A are not essential for these malaria parasites. However, parasitemia of the Duffy antigen knockout mice infected with P. yoelii 17XL remained constant from day 5 to 14 after infection, and then decreased, resulting in autotherapy. The treatment of P. yoelii 17XL-infected Duffy antigen knockout mice with anti-CD4 antibody increased the parasitemia 15 days after infection and the mice eventually died, indicating that CD-4-positive cells play an important role in the clearance of P. yoelii 17XL at the late stage of the infection.
Laboratory of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, 113-0033, Japan.
Nobuyoshi
N
Akimitsu
Hye-Sook
HS
Kim
Hiroshi
H
Hamamoto
Koushirou
K
Kamura
Nobuko
N
Fukuma
Nagisa
N
Arimitsu
Kanako
K
Ono
Yusuke
Y
Wataya
Motomi
M
Torii
Kazuhisa
K
Sekimizu
D000818:Animals; D051379:Mice; D000953:Antigens, Protozoan / Q000502:physiology; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D001789:Blood Group Antigens; D015704:CD4 Antigens / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004375:Duffy Blood-Group System / Q000235:genetics / Q000502:physiology; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D006021:Glycophorins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D008288:Malaria / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D018345:Mice, Knockout; D010962:Plasmodium berghei / Q000472:pathogenicity; D016788:Plasmodium chabaudi / Q000472:pathogenicity; D010967:Plasmodium yoelii / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D015800:Protozoan Proteins / Q000502:physiology; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000502:physiology
D000953:Antigens, Protozoan; D000961:Antilymphocyte Serum; D001789:Blood Group Antigens; D015704:CD4 Antigens; C066001:Duffy antigen binding protein, Plasmodium; D004375:Duffy Blood-Group System; D006021:Glycophorins; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; C483025:Ackr1 protein mouse