The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12.
The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidated except that all the azaserine-resistant strains isolated so far carry lesions in the aroP gene as a primary determinant. Here, we demonstrate that, in addition to AroP, the LIV-I/LS system, an ATP-binding cassette type transporter, is involved in azaserine sensitivity of E. coli, by genetic analysis and transport studies, in which Ki value for azaserine was determined to be approximately 10(-3) M.
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. koyataka@kais.kyoto-u.ac.jp
Takashi
T
Koyanagi
Takane
T
Katayama
Hideyuki
H
Suzuki
Hidehiko
H
Kumagai
D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001377:Azaserine / Q000378:metabolism* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007930:Leucine / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010649:Phenylalanine / Q000378:metabolism
D026905:Amino Acid Transport Systems; D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010649:Phenylalanine; D001377:Azaserine; D007930:Leucine