[Adipose tissue-derived stromal cells as vector for gene therapy in central nervous system].
OBJECTIVE
To investigate exogenous gene expressing ability of adipose tissue-derived stromal cell (ADSCs) and cell distribution after they were transplanted into brains, and to get the genetically modified cells for autografting.


METHODS
ADSCs were transfected by Ad5beta gal adenovirus containing a report gene, LacZ gene, then they were transplanted into the adult brain of rats, or ADSCs labeled by Hoechst33258 were transplanted into the adult brain of rats to investigate the migration and distribution of cells.


RESULTS
ADSCs showed a good expression of LacZ with X-gal staining after transfecting and transplantation into adult brains, and they could incorporate into the host brain tissues and no disruption was observed. These cells showed good compatibility with the host brains.


CONCLUSIONS
The results indicate that ADSCs could incorporate into host brains and express exogenous gene steadily when they were transplanted into adult brain tissues, no overproliferation and gliosis were identified, and ADSCs may be used as a therapeutic gene delivery vehicle in treating CNS disorders in humans.
Department of Neurosurgery, Chaozhou Central Hospital, Guangdong Province, Chaozhou 521021, China.
Li-ye
LY
Yang
Jia-kun
JK
Zheng
Guo-zhen
GZ
Hui
Li-he
LH
Guo
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001921:Brain / Q000601:surgery*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D015316:Genetic Therapy; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009474:Neurons / Q000166:cytology; D051381:Rats; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation*; D014162:Transfection