Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.
Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from Corynebacterium species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species 'Corynebacterium tuberculostearicum' and represented a distinct subline within the genus Corynebacterium. On the basis of both phenotypic and phylogenetic evidence, the formal description of Corynebacterium tuberculostearicum sp. nov. is proposed. The type strain of C. tuberculostearicum is Medalle XT (=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T).
Laboratoire de Génomique des Micro-organismes Pathogènes, Département Structure et Dynamique des Génomes, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.|Molecular Diagnostics Unit, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Cork, Ireland.|Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Carole
C
Feurer
Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Dominique
D
Clermont
Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
François
F
Bimet
Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Adina
A
Candréa
Unité de Génétique Mycobactérienne, Département Pathogénèse Microbienne, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Mary
M
Jackson
Laboratoire de Génomique des Micro-organismes Pathogènes, Département Structure et Dynamique des Génomes, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Philippe
P
Glaser
Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Chantal
C
Bizet
Collection de l'Institut Pasteur, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Catherine
C
Dauga
D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D003354:Corynebacterium Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005516:Food Microbiology; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S