Evaluation of a method based on liquid chromatography/electrospray tandem mass spectrometry for analyzing eight triazolic and pyrimidine fungicides in extracts of processed fruits and vegetables.
The feasibility of using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) for determining 8 fungicides (triadimenol, penconazole, propiconazole, hexaconazole, cyproconazole, myclobutanil, fenarimol, and bitertanol) in extracts of tomato puree and lemon juice concentrate has been evaluated. A miniaturized extraction-partition procedure requiring small amounts of nonchlorinated solvents has been used. The extracts (5 microL) were analyzed by LC/ESI-MS/MS without any previous cleanup step. Chromatographic determination has been performed using a C18 column and isocratic elution. Seventeen MS/MS transitions of precursor ions were monitored simultaneously (2 or 3 for each pesticide). The excellent selectivity and good linearity of the LC/MS/MS method allowed quantitation and identification at low levels (limits of quantitation <0.010 mg/kg), even in difficult matrixes, with a run time of only 1.5 min.
Sannino
Anna
A
Stazione Sperimentale per l'Industria delle Conserve Alimentari, Viale F. Tanara, Parma, Italy. annasannino@yahoo.it
D002853:Chromatography, Liquid; D002957:Citrus / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D007202:Indicators and Reagents; D018551:Solanum lycopersicum / Q000737:chemistry; D011743:Pyrimidines / Q000032:analysis*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
D005659:Fungicides, Industrial; D007202:Indicators and Reagents; D011743:Pyrimidines; D014230:Triazoles