[Application of RNAi technology to knockdown gene expression in vivo in mammalians].
Post-transcriptional gene silencing (PTGS) initiated by dsRNA, which result in specific degradation of homologous mRNA, is called RNAi. The discovery of RNAi greatly intrigued researchers, and was followed by a flood of papers that described the phenomenon and mechanism of RNAi. More excitingly, RNAi has recently been developed into a new tool, showing promising role in reverse genetics, gene therapy and anti-viral infection. This review provides the progress of the application of RNAi technology in mammalian animals.
Institute of Cardiovascular Sciences, Peking University, Beijing 100083.
Ai-Hong
AH
Zhang
Guo-Qing
GQ
Liu
D000818:Animals; D005786:Gene Expression Regulation; D020868:Gene Silencing; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D011494:Protein Kinases; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011494:Protein Kinases