A simple and cheaper in house varicella zoster virus antibody indirect ELISA.
We have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre. Also, the addition of an extra step with 8M urea allowed the assessment of VZV IgG avidity without excessive costs. The cost per sample to test VZV IgG was 2.7 times cheaper with our ELISA, allowing the testing of many samples without the burden of production of VZV antigen in the laboratory.
Ono
Erika
E
Division of Pediatric Infectious Diseases, Federal University of São Paulo, Sao Paulo, SP, Brazil.
Lafer
Manuel Mindlin
MM
Weckx
Lily Yin
LY
Granato
Celso
C
de Moraes-Pinto
Maria Isabel
MI
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D000915:Antibody Affinity / Q000276:immunology; D002644:Chickenpox / Q000175:diagnosis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D014645:Herpesvirus 3, Human / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D012680:Sensitivity and Specificity; D014613:Vaccines, Attenuated / Q000276:immunology
D000914:Antibodies, Viral; D022283:Herpesvirus Vaccines; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated