Significance of MiB-1 staining in smears with atypical glandular cells.
MiB-1 immunostaining may facilitate recognition of developing adenocarcinoma in cervical smear screening. In a retrospective analysis of prospectively collected data of 170 patients with atypical endocervical glandular cells and with repeat smears, archival Papanicolaou-stained smears were restained for MiB-1 and classified for the presence of preneoplastic changes of MiB-1 positive epithelial fragments. The results of classification based on MiB-1 positive epithelial fragments corresponded "roughly" with cytomorphological diagnoses. Of the 38 patients in which the primary smear was found to be MiB-1-positive, 12 of these persisted in the repeat smear. Thirty-eight of the 132 repeat smears of patients originally negative for MiB-1 pathology were positive. Of the total 50 MiB-1 positive repeat smears, four showed adenocarcinoma in situ on cytomorphological grounds. MiB-1 staining enhances detection of (pre)neoplastic changes. This approach does not destroy the morphology of the original smear and can be applied to routine material.
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands.
Mathilde E
ME
Boon
Astrid
A
Vinkestein
Annette
A
van Binsbergen-Ingelse
Caroline
C
van Haaften
D014408:Biomarkers, Tumor / Q000032:analysis; D004714:Endometrial Hyperplasia / Q000175:diagnosis / Q000473:pathology; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D065006:Papanicolaou Test; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D013194:Staining and Labeling; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology; D014626:Vaginal Smears
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen