An improved method for the solution cyclization of peptides under pseudo-high dilution conditions.
Depending on the ring size, the cyclization of peptides often is accompanied by dimerization or cyclodimerization. Hence, these macrocyclizations have to be performed under high dilution conditions. Efficient cyclization of peptides in solution with a minimum amount of solvent succeeds, when a dual syringe pump is used to simultaneously add the linear peptide precursor and a coupling reagent from two separate syringes.
Organic and Bioorganic Chemistry, Department of Chemistry, University of Bielefeld, P.O. Box 100131, 33501 Bielefeld, Germany.
Miroslav
M
Malesevic
Ulf
U
Strijowski
Dirk
D
Bächle
Norbert
N
Sewald
D045424:Complex Mixtures / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008832:Microchemistry / Q000379:methods*; D046912:Multiprotein Complexes / Q000138:chemical synthesis* / Q000302:isolation & purification*; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000302:isolation & purification*; D012996:Solutions
D045424:Complex Mixtures; D003432:Cross-Linking Reagents; D046912:Multiprotein Complexes; D010456:Peptides, Cyclic; D012996:Solutions