Long term transgene expression by hepatocytes transduced with retroviral vectors requires induction of immune tolerance to the transgene.
OBJECTIVE
Gene therapy for inherited liver diseases requires permanent expression of the therapeutic gene. However, in vivo liver transduction with retroviral vectors triggers an immune elimination of transduced hepatocytes. Here we investigated whether immune response could be prevented by treatment with compounds known to induce tolerance in organ transplantation: CTLA4Ig and LF-15-0195.


METHODS
CTLA4Ig was administered either via i.p. injection of the drug or by i.m. injection of recombinant adenoviruses encoding CTLA4Ig. LF-15-0195 was administered i.p. All animals were subjected to partial hepatectomy and received beta-galactosidase retroviral vectors intravenously. Appearance of anti-beta-galactosidase antibodies was monitored and the number of positive hepatocytes was assessed at day 7 and at sacrifice.


RESULTS
No beta-galactosidase antibodies were detected as long as CTLA4Ig was detectable in serum. Short-term treatment with CTLA4Ig induced tolerance in a significant proportion of animals only at high dose (1 mg/kg). Administration of CTLA4Ig adenovectors resulted in prolonged secretion of CTLA4Ig and permanent absence of anti-beta-galactosidase antibodies. LF-15-0915 administration achieved tolerance in some animals.


CONCLUSIONS
In conclusion, manipulation of the immune system at the time of virus delivery using clinically relevant tolerance-inducing protocols is a promising approach to achieve long term expression after retrovirus-mediated gene transfer to the liver.
Puppi
Juliana
J
Biothérapie Hépatique, CIC-INSERM 00-04, CHU Hôtel Dieu, 44093 Nantes cedex 01, France.
Guillonneau
Carole
C
Pichard
Virginie
V
Bellodi-Privato
Marta
M
Cuturi
Maria Cristina
MC
Anegon
Ignacio
I
Ferry
Nicolas
N
D000069594:Abatacept; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D015870:Gene Expression / Q000276:immunology*; D005822:Genetic Vectors; D006146:Guanidines / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D007108:Immune Tolerance / Q000187:drug effects; D018796:Immunoconjugates / Q000235:genetics / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae / Q000235:genetics; D013997:Time Factors; D014161:Transduction, Genetic; D019076:Transgenes / Q000276:immunology*
D006146:Guanidines; D018796:Immunoconjugates; D007166:Immunosuppressive Agents; C434185:LF 150195; D000069594:Abatacept