Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR.
Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.
Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, 1 Veterans Dr., Minneapolis, MN 55417, USA. johns007@umn.edu
James R
JR
Johnson
Krista
K
Owens
Montse
M
Sabate
Guillem
G
Prats
D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis; D005798:Genes, Bacterial / Q000235:genetics; D005843:Geography; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D017931:DNA Primers