Detection of Haemophilus influenzae type b by real-time PCR.
A real-time PCR assay targeting the capsulation locus of Haemophilus influenzae type b (Hib) was developed. The linear detection range was from 1 to 10(6) microorganisms per reaction mixture. No H. influenzae other than Hib or any other control bacteria typically found in the upper respiratory tract was detected.
Marty
Adrian
A
Division of Infectious Diseases, University Children's Hospital of Zurich, Steinwiesstr. 75, CH-8032 Zurich, Switzerland.
Greiner
Oliver
O
Day
Philip J R
PJ
Gunziger
Sibylle
S
Mühlemann
Kathrin
K
Nadal
David
D
D001483:Base Sequence; D003199:Computer Systems; D017931:DNA Primers; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers