Inducible gene expression in mammalian cells and mice.
Inducible expression of desired transgenes in mammalian cells and animals is a current priority in basic and applied research, biopharmaceutical manufacturing, gene therapy, and tissue engineering, as well as in drug discovery. Among the most prominent human-compatible transgene control technologies are engineered promoter/transactivator configurations that adjust heterologous target gene transcription in response to clinically licensed antibiotics (tetracyclines, streptogramins, macrolides). In this chapter we provide a detailed case study on macrolide-inducible expression of the human model glycoprotein SEAP (human placental secreted alkaline phosphatase) in transgenic Chinese hamster ovary (CHO) cell cultures or following implantation of microencapsulated CHO cells into mice.
Institute of Biotechnology, Swiss Federal Institute of Technology, Zurich, Switzerland.
Wilfried
W
Weber
Martin
M
Fussenegger
D000818:Animals; D016466:CHO Cells / Q000637:transplantation; D002460:Cell Line; D006224:Cricetinae; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter; D005822:Genetic Vectors; D051379:Mice; D008822:Mice, Transgenic / Q000378:metabolism; D010957:Plasmids; D014162:Transfection / Q000379:methods*