Phenazine-1-carboxylic acid is negatively regulated and pyoluteorin positively regulated by gacA in Pseudomonas sp. M18.
The biosynthesis of antimicrobial metabolites is controlled by the GacS/GacA two-component regulatory system in Pseudomonas species. The production of phenazine-1-carboxylic acid and pyoluteorin is differentially regulated by GacA in Pseudomonas sp. M18. Pyoluteorin was reduced to nondetectable level in culture of the gacA insertional mutant strain M18G grown in King's medium B broth, whereas phenazine-1-carboxylic acid production was increased 30-fold over that of the wild-type strain. Production of both antibiotics was restored to wild-type levels after complementation in trans with the wild-type gacA gene. Expression of the translational fusions phzA'-'lacZ and pltA'-'lacZ confirmed the effect of GacA on both biosynthetic operons.
College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, PR China.
Yihe
Y
Ge
Xianqing
X
Huang
Sulian
S
Wang
Xuehong
X
Zhang
Yuquan
Y
Xu
D000222:Adaptation, Physiological; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter / Q000235:genetics / Q000502:physiology; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D010619:Phenazines / Q000378:metabolism*; D010636:Phenols; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism*; D011758:Pyrroles; D015398:Signal Transduction; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; D010619:Phenazines; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin; C037165:1-phenazinecarboxylic acid; D001616:beta-Galactosidase