Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea.
Two actinomycete strains, DSA1T and HD9T, were isolated from a potato tuber and soil from a potato-cultivating field in Jeju, Korea, respectively. A comprehensive 16S rDNA analysis revealed that the isolates belong to the genus Kribbella and share 97.7-98.6% sequence similarity to Kribbella species. The strains also contained typical chemotaxonomic markers of the genus Kribbella: ll-diaminopimelic acid, alanine, glycine and glutamic acid in the cell wall peptidoglycan; mannose, glucose, galactose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as characteristic phospholipids; and the major menaquinone MK-9 (H4). DNA-DNA hybridization experiments showed that the isolates represented two distinct genomic species. A number of phenotypic properties can be used to differentiate the two isolates from Kribbella species. On the basis of polyphasic evidence, two novel species are proposed: Kribbella solani sp. nov. for strain DSA1T (=KACC 20196T=JCM 12205T) and Kribbella jejuensis sp. nov. for strain HD9T (=KACC 20266T=JCM 12204T).
Song
Jaekyeong
J
Department of Biological Science, Myong Ji University, Yongin 449-728, Korea.|KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea.
Kim
Byung-Yong
BY
KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea.
Hong
Seung-Beom
SB
KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea.
Cho
Hyun-Suk
HS
KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea.
Sohn
Kyounghee
K
School of Biological Sciences, Seoul National University, Seoul, 151-742, Korea.
Chun
Jongsik
J
School of Biological Sciences, Seoul National University, Seoul, 151-742, Korea.
Suh
Joo-Won
JW
Department of Biological Science, Myong Ji University, Yongin 449-728, Korea.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D020459:Genes, rRNA / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D035281:Plant Tubers / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000382:microbiology*; D024482:Vitamin K 2 / Q000032:analysis
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2