Quantification of MDR-1 gene expression in canine tissues by real-time reverse transcription quantitative polymerase chain reaction.
MDR-1 gene product mediated multidrug resistance is thought to play a major role in the outcome of chemotherapy in some canine tumors, especially malignant lymphoma. In the present study, MDR-1 RNA expression in normal lymph node and liver tissue as well as in tumor biopsies from 23 dogs with lymphomas and two dogs with liver tumors was measured by real-time RT-quantitative PCR. MDR-1 gene expression was detected in all samples analyzed. Comparably high MDR-1 RNA levels were measured in all normal liver tissues, one of the lymphomas and a cholangiocarcinoma. MDR-1 expression levels in canine lymphomas were found to vary over a wide range with most tumors expressing relative low levels. Interestingly, gastrointestinal lymphomas expressed higher MDR-1 RNA levels than multicentric lymphomas (p = 0.03). In conclusion, real-time RT-quantitative PCR appears to be a suitable method for sensitive and quantitative determination of MDR-1 gene expression in canine normal and neoplastic tissues.
Culmsee
K
K
Clinic of Small Animals, School of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany. katja.culmsee@tiho-hannover.de
Gruber
A D
AD
von Samson-Himmelstjerna
G
G
Nolte
I
I
D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D019450:Genes, MDR / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000662:veterinary*; D008198:Lymph Nodes / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary
D012333:RNA, Messenger