AMID is a p53-inducible gene downregulated in tumors.
AMID, also called PRG3, is an AIF-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. In this report, we demonstrated that human AMID gene promoter was activated by p53 in reporter gene assays. Chromatin immunoprecipitation experiments indicated that p53 could bind to human AMID promoter. Deletion mutagenesis indicated that human AMID promoter contains two p53-responsive elements. Furthermore, expression array analysis indicated that human AMID mRNA expression was downregulated in a majority of human tumors. Our findings point to the possibility that AMID is a p53-downstream gene involved in tumorigenesis.
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Min
M
Wu
Liang-Guo
LG
Xu
Tian
T
Su
Yang
Y
Tian
Zhonghe
Z
Zhai
Hong-Bing
HB
Shu
D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D017930:Genes, Reporter; D016158:Genes, p53; D006801:Humans; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics
C462504:ferroptosis suppressor protein 1, human; D051017:Apoptosis Regulatory Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger