[Expression of proteinase cathepsin L1 gene of Schistosoma japonicum in Escherichia coli].
OBJECTIVE
To express the proteinase cathepsin L1 gene of Schistosoma japonicum (SjCL1) in Escherichia coli JM109 cells.


METHODS
The SjCL1 gene was amplified from the recombinant plasmid pcDNA3-SjCL1 by PCR. The gene was cloned into a prokaryotic expression vector pGEX4T-1 to construct a recombinant plasmid pGEX-SjCL1. The E. coli JM109 cells were transformed with the recombinant plasmid pGEX-SjCL1 and the transformants were induced by IPTG to express the recombinant protein, the target protein was then identified by SDS-PAGE and Western blotting.


RESULTS
A 1 kb length PCR product was obtained and a recombinant plasmid pGEX-SjCL1 was constructed. The expression product was detected by SDS-PAGE and Western blotting and an expression band about 62000 was found.


CONCLUSIONS
The SjCL1 gene is effectively expressed in the E. coli JM109 cells.
Yi
Bing
B
Department of Parasitology, Zhong Shan Medical College, Sun Yat-Sen University, Guangzhou 510099, China.
Li
Zhuo-ya
ZY
He
Ai
A
Zheng
Xiao-ying
XY
Zheng
Bin
B
Zhou
Hao-jie
HJ
Wang
Yi
Y
Zhang
Rui-lin
RL
Yu
Nan
N
Zhan
Xi-mei
XM
D000818:Animals; D002403:Cathepsins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D002403:Cathepsins