Modified technique of ureteroureterostomy in rat kidney transplantation.
Different strain combinations of rats are available to study immunological and transplant-related problems in the models of kidney transplantation. Although numerous modifications of surgical techniques for ureteric reconstruction are evaluated in order to reduce complications and to extend long-term survival, ureteric complications still occur frequently, especially when the difference in diameter of both donor and host ureters is disproportionate. Instead of using the current nonsplinted ureteroureterostomy (method A), a versatile and rapid technical modification (method B) was developed to perform reconstruction of ureters with disproportionate diameters. The overall incidence of ureteric complications was 80% (8/10) using method A, whereas this rate was significantly reduced to 15% (3/20) using method B (P < 0.001). Our modification proves the feasibility of nonsplinted ureteroureterostomy in a technical, highly demanding rat model of kidney transplantation with an acceptable rate of ureteric complication, considering the disproportionate difference in diameter between the host and donor ureters.
Division of Organ Transplantation, University of Wisconsin Hospital and Clinics, Madison, WI 53792-3236, USA.
Alexander
A
Pietsch
Philipp C
PC
Nett
Hans W
HW
Sollinger
Debra A
DA
Hullett
D000714:Anastomosis, Surgical; D000818:Animals; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D051381:Rats; D014513:Ureter / Q000601:surgery*; D014519:Ureterostomy / Q000379:methods*