Persistence of human immunodeficiency virus type 1 subtype B DNA in dried-blood samples on FTA filter paper.
The stability of human immunodeficiency virus type 1 (HIV-1) DNA in whole blood collected on filter paper (FTA Card) was evaluated. After >4 years of storage at room temperature in the dark our qualitative assay detected virus at a rate similar to that of our initial test (58 of 60, 97%; P = 0.16), suggesting long-term HIV-1 DNA stability.
Department of Pediatrics, University of Washington, Seattle, USA.
Chung-Chen
CC
Li
Ingrid A
IA
Beck
Kristy D
KD
Seidel
Lisa M
LM
Frenkel
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis*; D001800:Blood Specimen Collection / Q000379:methods; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D010209:Paper; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004279:DNA, Viral