Elemental sulfur: toxicity in vivo and in vitro to bacterial luciferase, in vitro yeast alcohol dehydrogenase, and bovine liver catalase.
The aim of this research was to analyze the effects and the modes of action of elemental sulfur (S(0)) in bioluminescence and respiration of Vibrio fischeri cells and the enzymes crude luciferase, pure catalase, and alcohol dehydrogenase (ADH). Metallic copper removed sulfur and reduced the toxicity of acetone extracts of sediment samples analyzed in the bioluminescence test. The sulfur inhibition of cell bioluminescence was noncompetitive with decanal, the luciferase substrate; reversible, with maximum toxicity after 15 min (EC(50) = 11.8 microg/L); and almost totally recovered after 2 h. In vitro preincubation of crude luciferase extract with sulfur (0.28 ppm) weakly inhibited bioluminescence at 5 min, but at 30 min the inhibition reached 60%. Increasing the concentration of sulfur in the parts per million concentration range in vitro decreased bioluminescence, which was not constant, but depended on exposure time, and no dead-end/total inhibition was observed. The redox state of enzymes in the in vitro system significantly affected inhibition. Hydrogen peroxide restored fully and the reducing agent dithiothreitol, itself toxic, restored only partially luciferase activity in the presence of sulfur. Sulfur (5.5 ppm) slightly inhibited ADH and catalase, and dithiothreitol enhanced sulfur inhibition. High sulfur concentrations (2.2 ppm) inhibited the bioluminescence and enhanced the respiration rate of V. fischeri cells. Elemental sulfur data were interpreted to show that sulfur acted on at least a few V. fischeri cell sites: reversibly modifying luciferase at sites sensitive to/protected by oxidative and reducing agents and by affecting electron transport processes, resulting in enhanced oxygen consumption. Sulfur together with an enzyme reducing agent inhibited the oxidoreductive enzymes ADH and catalase, which have --SH groups, metal ion cofactors, or heme, respectively, in their active centers.
Department of Biochemistry and Biophysics, Vilnius University, Ciurlionio Str. 21, LT-2009 Vilnius, Lithuania. Anolda.Cetkauskaite@gf.vu.lt
Anolda
A
Cetkauskaite
Piia
P
Pessala
Anders
A
Södergren
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors*; D000447:Aldehydes; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors*; D002417:Cattle; D019015:Geologic Sediments / Q000737:chemistry; D008099:Liver / Q000201:enzymology; D008156:Luciferases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D010101:Oxygen Consumption / Q000187:drug effects; D013455:Sulfur / Q000633:toxicity*; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000447:Aldehydes; D014874:Water Pollutants, Chemical; C021170:decanaldehyde; D013455:Sulfur; D000426:Alcohol Dehydrogenase; D002374:Catalase; D008156:Luciferases