Bilaterally identical monoclonality in a case of primary intraocular lymphoma.
OBJECTIVE
To report a case of primary intraocular lymphoma possessing bilaterally identical monoclonal rearrangements of the immunoglobulin heavy chain (IgH) gene.


METHODS
Observational case report.


METHODS
A 78-year-old woman with bilateral vitritis, subretinal infiltrates in the right eye, and no involvement of the central nervous system (CNS) was diagnosed histologically with diffuse large B-cell lymphoma by a transvitreal subretinal biopsy of the right eye. One month later, vitrectomy was performed on the left eye due to increased vitreous opacity. DNA was extracted from a vitrectomy specimen from each eye, and the third complementarity-determining region of IgH gene was analyzed by polymerase chain reaction and direct sequencing.


RESULTS
Monoclonal rearrangements of IgH gene with identical DNA sequence were detected in both eyes.


CONCLUSIONS
Bilaterally identical monoclonality was detected in a case of primary intraocular lymphoma with no CNS involvement.
Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan. eyetomo@kenroku.kanazawa-u.ac.jp
Tomomi
T
Higashide
Masayuki
M
Takahira
Hirokazu
H
Okumura
Makoto
M
Torisaki
Mayumi
M
Sakurai
Yutaka
Y
Shirao
Kazuhisa
K
Sugiyama
D000368:Aged; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D016133:Polymerase Chain Reaction; D019572:Retinal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D017422:Sequence Analysis, DNA; D014821:Vitrectomy
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains