Storage and secretion of the peritrophic matrix protein Ag-Aper1 and trypsin in the midgut of Anopheles gambiae.
The gene Ag-Aper1 encodes a peritrophic matrix (PM) protein from the mosquito Anopheles gambiae. Ag-Aper1 gene expression and protein localization in the mosquito midgut were studied during the course of a blood meal. Ag-Aper1 mRNA abundance does not change appreciably during the course of blood ingestion and digestion. Prior to a blood meal, the protein is stored in secretory vesicles of midgut epithelial cells. Moreover, Ag-Aper1 colocalizes to the same secretory vesicles as trypsin, indicating that these proteins use a common secretory pathway. Blood feeding triggers the secretion of vesicle contents into the midgut lumen, after which Ag-Aper1 is incorporated into the PM. Newly synthesized Ag-Aper1 protein was again detected within the midgut epithelial cells at 60 h after blood ingestion.
Devenport
M
M
Department of Genetics, Case Western Reserve University, Cleveland, OH, USA.
Fujioka
H
H
Jacobs-Lorena
M
M
D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D000906:Antibodies / Q000378:metabolism; D001769:Blood / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004063:Digestion / Q000502:physiology; D004064:Digestive System / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism*
D000906:Antibodies; C113435:Aper1 protein, Anopheles gambiae; D002352:Carrier Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D014357:Trypsin