Identification of O2-induced peptides in an obligatory anaerobe, Clostridium acetobutylicum.
Clostridium acetobutylicum DSM792 (=ATCC824), a solvent producing obligate anaerobe, grew well after a shift in growth conditions from anoxic to microoxic at the mid exponential phase. In two-dimensional gel electrophoresis, a spot migrating at 45 kDa and three spots at 23 kDa accumulated after 30 min of flushing with 5% O(2)/95% N(2). Based on peptide mass fingerprints, the 45 kDa polypeptide was determined to be NP_347663 (A-type flavoprotein homologue) and the 23 kDa polypeptides were determined to be NP_350180 or NP_350181 (novel type rubrerythrin homologue). Northern blot analysis indicated that the expressions of these peptide transcripts were upregulated within 10 min after flushing with 5% O(2)/95% N(2).
Kawasaki
Shinji
S
Department of Biosciences, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan. kawashin@nodai.ac.jp
Ishikura
Jun
J
Watamura
Yusuke
Y
Niimura
Youichi
Y
D000595:Amino Acid Sequence; D001421:Bacteria, Anaerobic / Q000378:metabolism; D001426:Bacterial Proteins; D003013:Clostridium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005288:Ferredoxins; D016680:Genome, Bacterial; D006422:Hemerythrin; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D010100:Oxygen / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012416:Rubredoxins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D010455:Peptides; D012416:Rubredoxins; C055857:rubrerythrins; D010100:Oxygen