ST64B is a defective bacteriophage in Salmonella enterica serovar Typhimurium DT64 that encodes a functional immunity region capable of mediating phage-type conversion.
The Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) defective bacteriophage ST64B has a putative immunity (immC) region consisting of cI, cro and cII-like genes. Since ST64B is widespread in S. Typhimurium, studies were undertaken to determine whether this region might be functional and influence phage typing results. Cloning of ST64B immC-like genes and their subsequent expression in S. Typhimurium DTs showed that this region is able to mediate phage-type conversion in DTs 41 and 44. This confirms the functionality of the immC region and the patterns of lysis produced by phage typing are consistent with the predicted mechanism of action of the encoded protein products.
Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, P.O. Box 14 Rundle Mall, Adelaide, South Australia 5000, Australia.
Carly P
CP
Tucker
Michael W
MW
Heuzenroeder
D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000302:isolation & purification* / Q000502:physiology*; D003001:Cloning, Molecular; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D005814:Genes, Viral; D010641:Phenotype; D017902:Podoviridae / Q000302:isolation & purification / Q000502:physiology*; D012483:Salmonella Phages / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012486:Salmonella typhimurium / Q000821:virology*; D017385:Sequence Homology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
D014157:Transcription Factors; D014764:Viral Proteins; C039000:cII protein, bacteriophage lambda