Nondestructive observation of bovine milk by NMR spectroscopy: analysis of existing States of compounds and detection of new compounds.
In this study were successfully observed the one- (1H, 13C) and two-dimensional (1H-13C, 1H-15N, 1H-31P) NMR spectra of milk directly without any pretreatment. The signals of each NMR spectrum were assigned, and their existing states were also analyzed. Lactose existed in a free state in milk. The signals due to the butyric acid chain can be assigned among the other fatty acid chains. Monounsaturated fatty acid (oleic acid chains) and polyunsaturated fatty acid chains (linoleic and linolenic acid) were assigned by their characteristic signals. The signals from citrate, N-acetylcarbohydrates, and lecithin could be observed directly in the 1H-13C HSQC NMR spectra; the assignment of their signals was made through the 1H-13C, 1H-15N, and 1H-31P HMBC spectra of extracted milk. Signals from creatine and N-acetylcarbohydrates were detected for the first time.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Fangyu
F
Hu
Kazuo
K
Furihata
Mie
M
Ito-Ishida
Shuichi
S
Kaminogawa
Masaru
M
Tanokura
D000107:Acetylation; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D019343:Citric Acid / Q000032:analysis; D003401:Creatine / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007785:Lactose / Q000032:analysis; D008055:Lipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000032:analysis
D002241:Carbohydrates; D005227:Fatty Acids; D008055:Lipids; D010713:Phosphatidylcholines; D019343:Citric Acid; D007785:Lactose; D003401:Creatine