Electrochemical detector for microchip electrophoresis of poly(dimethylsiloxane) with a three-dimensional adjustor.
This paper presents an electrochemical detector for poly(dimethylsiloxane) (PDMS) microchip CE with a three-dimensional adjustor which makes it possible to accurately align a separate working electrode that can be easily fabricated in laboratory to the uncertain PDMS microchannel outlet. The substantial influence of the electrode-PDMS microchannel distance was investigated. The optimal electrode-outlet distance was found to be 10 microm for the PDMS microchannel with the width of 50 microm due to its relatively slow electroosmotic flow. Adrenaline and catechol were well separated, with a linear response range from 20 microM to 1 mM, and a detection limit of 2 microM for catechol, using carbon disk electrode (diameter of 300 microm). Furthermore, arginine and histidine can be well separated and detected directly in the PDMS microchannel using a Cu disk electrode (diameter of 150 microm).
Department of Chemistry, The Laboratory of Life Analytical Chemistry, Nanjing University, Nanjing 210093, China.
Ning
N
Bao
Jing-Juan
JJ
Xu
Yue-Hua
YH
Dou
Yan
Y
Cai
Hong-Yuan
HY
Chen
Xing-Hua
XH
Xia
D004129:Dimethylpolysiloxanes / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D012828:Silicones / Q000032:analysis*
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon