Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples.
Three strains of a hitherto unknown, Gram-negative, anaerobic coccus were isolated from human samples. At the phenotypic level, the isolates displayed all the characteristics of bacteria belonging to the genus Veillonella. Sequence analysis revealed that the three strains shared >99.5% similarity in 16S rDNA sequence and >98.4% similarity in dnaK sequence. The three unknown strains formed a separate subclade that was clearly remote from Veillonella species of human and animal origin. Based on these results, the three strains were considered to represent a novel species within the genus Veillonella, for which the name Veillonella montpellierensis is proposed. The type strain of the species is ADV 281.99T (=CIP 107992T=CCUG 48299T).
Jumas-Bilak
Estelle
E
Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France.
Carlier
Jean-Philippe
JP
Centre National de Référence des Bactéries Anaérobies et du Botulisme, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France.
Jean-Pierre
Hélène
H
Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Teyssier
Corinne
C
Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France.
Gay
Bernard
B
Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Campos
Josiane
J
Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Marchandin
Hélène
H
Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
D000328:Adult; D000653:Amniotic Fluid / Q000382:microbiology; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005750:Gastric Juice / Q000382:microbiology; D020459:Genes, rRNA; D005840:Gentian Violet; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D008854:Microscopy, Electron; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014678:Veillonella / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D018832:Molecular Chaperones; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet