Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes.
OBJECTIVE
T-Cell lymphomas constitute heterogeneous and aggressive tumors in which pathogenic alterations remain largely unknown. Expression profiling has demonstrated to be a useful tool for molecular classification of tumors.


METHODS
Using DNA microarrays (CNIO-OncoChip) containing 6386 cancer-related genes, we established the expression profiling of T-cell lymphomas and compared them to normal lymphocytes and lymph nodes.


RESULTS
We found significant differences between the peripheral and lymphoblastic T-cell lymphomas, which include a deregulation of nuclear factor-kappaB signaling pathway. We also identify differentially expressed genes between peripheral T-cell lymphoma tumors and normal T lymphocytes or reactive lymph nodes, which could represent candidate tumor markers of these lymphomas. Additionally, a close relationship between genes associated to survival and those that differentiate among the stages of disease and responses to therapy was found.


CONCLUSIONS
Our results reflect the value of gene expression profiling to gain insight about the molecular alterations involved in the pathogenesis of T-cell lymphomas.
Human Genetics Department, Bioinformatics Unit, Genomic Analysis Unit, and Genomics Unit, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain. bmartinez@cnio.es
Beatriz
B
Martinez-Delgado
Barbara
B
Meléndez
Marta
M
Cuadros
Javier
J
Alvarez
Jose Maria
JM
Castrillo
Ana
A
Ruiz De La Parte
Manuela
M
Mollejo
Carmen
C
Bellas
Ramon
R
Diaz
Luis
L
Lombardía
Fatima
F
Al-Shahrour
Orlando
O
Domínguez
Alberto
A
Cascon
Mercedes
M
Robledo
Carmen
C
Rivas
Javier
J
Benitez
D002470:Cell Survival; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D005810:Multigene Family; D016328:NF-kappa B / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016328:NF-kappa B