INFgamma stimulates arginine transport through system y+L in human monocytes.
Freshly isolated human monocytes transport L-arginine mostly through a sodium independent, NEM insensitive pathway inhibited by L-leucine in the presence, but not in the absence of sodium. Interferon-gamma (IFNgamma) stimulates this pathway, identifiable with system y+L, and markedly enhances the expression of SLC7A7, the gene that encodes for system y+L subunit y+LAT1, but not of SLC7A6, that codes for the alternative subunit y+LAT2. System y+ plays a minor role in arginine uptake by monocytes and the expression of system y+-related genes, SLC7A1 and SLC7A2, is not changed by IFNgamma. These results demonstrate that system y+L is sensitive to IFNgamma.
Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale e Clinica, Plesso Biotecnologico Integrato, Università degli Studi di Parma, Via Volturno 39, 43100 Parma, Italy.
Bianca Maria
BM
Rotoli
Ovidio
O
Bussolati
Roberto
R
Sala
Amelia
A
Barilli
Enrica
E
Talarico
Gian C
GC
Gazzola
Valeria
V
Dall'Asta
D027241:Amino Acid Transport System y+L; D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D005033:Ethylmaleimide / Q000494:pharmacology; D027301:Fusion Regulatory Protein 1, Light Chains / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000494:pharmacology
D027241:Amino Acid Transport System y+L; D026923:Amino Acid Transport Systems, Basic; D027301:Fusion Regulatory Protein 1, Light Chains; D011994:Recombinant Proteins; C469951:SLC7A6 protein, human; C421261:SLC7A7 protein, human; D007371:Interferon-gamma; D001120:Arginine; D012964:Sodium; D007930:Leucine; D005033:Ethylmaleimide