Bacillus subtilis YhcR, a high-molecular-weight, nonspecific endonuclease with a unique domain structure.
In a continuing effort to identify ribonucleases that may be involved in mRNA decay in Bacillus subtilis, fractionation of a protein extract from a triple-mutant strain that was missing three previously characterized 3'-to-5' exoribonucleases (polynucleotide phosphorylase [PNPase], RNase R, and YhaM) was undertaken. These experiments revealed the presence of a high-molecular-weight nuclease encoded by the yhcR gene that was active in the presence of Ca(2+) and Mn(2+). YhcR is a sugar-nonspecific nuclease that cleaves endonucleolytically to yield nucleotide 3'-monophosphate products, similar to the well-characterized micrococcal nuclease of Staphylococcus aureus. YhcR appears to be located principally in the cell wall and is likely to be a substrate for a B. subtilis sortase. Zymogram analysis suggests that YhcR is the major Ca(2+)-activated nuclease of B. subtilis. In addition to having a unique overall domain structure, YhcR contains a hitherto unknown structural domain that we have named "NYD," for "new YhcR domain."
Oussenko
Irina A
IA
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine of New York University, New York, NY 10029, USA.
Sanchez
Roberto
R
Bechhofer
David H
DH
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins; D002118:Calcium / Q000494:pharmacology; D002473:Cell Wall / Q000201:enzymology; D003001:Cloning, Molecular; D003067:Coenzymes / Q000494:pharmacology; D017124:Conserved Sequence; D003546:Cysteine Endopeptidases; D015226:Dinucleoside Phosphates / Q000378:metabolism; D004720:Endonucleases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005798:Genes, Bacterial; D008345:Manganese / Q000494:pharmacology; D008836:Micrococcal Nuclease / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics; D017434:Protein Structure, Tertiary; D012260:Ribonucleases / Q000378:metabolism; D016415:Sequence Alignment
D001426:Bacterial Proteins; D003067:Coenzymes; D015226:Dinucleoside Phosphates; D008345:Manganese; D019881:Aminoacyltransferases; C120542:sortase A; D011117:Polyribonucleotide Nucleotidyltransferase; D004720:Endonucleases; D012260:Ribonucleases; C489660:YhcR protein, Bacillus subtilis; D008836:Micrococcal Nuclease; D003546:Cysteine Endopeptidases; D002118:Calcium