Gene targeting: attention to detail.
When it comes to silencing genes in mice, not all approaches are equal. An example published in this issue of Cell (Patrucco et al., 2004) suggests that caution should be used when validating potential drug targets by genetic disruption.
Vanhaesebroeck
Bart
B
Ludwig Institute for Cancer Research, London, United Kingdom.
Rohn
Jennifer L
JL
Waterfield
Michael D
MD
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D018390:Gene Targeting; D051379:Mice; D005810:Multigene Family; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*
D000242:Cyclic AMP; D019869:Phosphatidylinositol 3-Kinases