DNA ploidy and S-phase fraction in the patients of chronic HCV and hepatocellular carcinomas.
Four hundred blue Fulgen-stained nuclei were measured from each lesion by using DNA image cytometry. The histopathological and cytopathological observations revealed that (52 cases, 69.3%) had a variable degrees of chronic hepatitis, (12cases 16 %) were emerging into cirrhosis, while (11 cases 14.7%) represented different grades of HCC. Most of the cases with minimal or mild chronic hepatitis were female, while most of male had moderate or severe chronic hepatitis, cirrhosis and HCC. DNA image analysis data gave the support of to the histological observations. All of chronic hepatitis C and cirrhotic cases showed normal diploid and/or tetraploid histograms, although increasing S-phase fraction s' values of the highly diseased chronic hepatitis and cirrhotic cases. Hepatocellular carcinomas and one cirrhotic case only revealed aneuploidy (diploid and tetraploid), while one case of poorly differentiated HCC revealed multiploid histogram. So, histopathological severity in cases of progressive chronic hepatitis seems to be associated with the age and sex of Egyptian society. Also, demonstrates the potential usefulness of image cytometry for the evaluation of the different histopathological problems.
Sanad
Samia M
SM
Department of Zoology, Faculty of Science, Zagazig University, Egypt.
Mangoud
Amal M
AM
Shalabi
Amro A
AA
Saber
Mahmoud
M
Fouad
Mahmoud A H
MA
D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000254:growth & development*; D019698:Hepatitis C, Chronic / Q000235:genetics / Q000473:pathology* / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology; D008297:Male; D011003:Ploidies; D016196:S Phase
D004279:DNA, Viral