Proteomics of calcium-signaling components in plants.
Calcium functions as a versatile messenger in mediating responses to hormones, biotic/abiotic stress signals and a variety of developmental cues in plants. The Ca(2+)-signaling circuit consists of three major "nodes"--generation of a Ca(2+)-signature in response to a signal, recognition of the signature by Ca2+ sensors and transduction of the signature message to targets that participate in producing signal-specific responses. Molecular genetic and protein-protein interaction approaches together with bioinformatic analysis of the Arabidopsis genome have resulted in identification of a large number of proteins at each "node"--approximately 80 at Ca2+ signature, approximately 400 sensors and approximately 200 targets--that form a myriad of Ca2+ signaling networks in a "mix and match" fashion. In parallel, biochemical, cell biological, genetic and transgenic approaches have unraveled functions and regulatory mechanisms of a few of these components. The emerging paradigm from these studies is that plants have many unique Ca2+ signaling proteins. The presence of a large number of proteins, including several families, at each "node" and potential interaction of several targets by a sensor or vice versa are likely to generate highly complex networks that regulate Ca(2+)-mediated processes. Therefore, there is a great demand for high-throughput technologies for identification of signaling networks in the "Ca(2+)-signaling-grid" and their roles in cellular processes. Here we discuss the current status of Ca2+ signaling components, their known functions and potential of emerging high-throughput genomic and proteomic technologies in unraveling complex Ca2+ circuitry.
Reddy
Vaka S
VS
Department of Biology and Program in Cell and Molecular Biology, Colorado State University, 200 West Lake Street, Fort Collins, CO 80523, USA.
Reddy
Anireddy S N
AS
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D020013:Calcium Signaling; D008954:Models, Biological; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D010944:Plants / Q000378:metabolism*; D011485:Protein Binding; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D015398:Signal Transduction
D010940:Plant Proteins; D020543:Proteome