Serum P-selectin level during controlled ovarian hyperstimulation--a preliminary report.
OBJECTIVE
To measure levels of serum P-selectin in patients undergoing controlled ovarian hyperstimulation (COH) cycles and to determine their possible correlation to COH variables.


METHODS
Large university-based infertility and in vitro fertilization unit.


METHODS
Fourteen consecutive patients undergoing our routine COH protocol for unexplained infertility.


METHODS
Blood was drawn three times during the COH cycle: (1) day 2 or 3 of the menstrual cycle, before gonadotropin treatment (Day-0); (2) day of or prior to human chorionic gonadotropin (hCG) administration (Day-hCG); and (3) day of ovulation (Day-OVU). Serum levels of sex steroids and P-selectin were compared among the three time points. P-selectin was measured with a commercial quantitative sandwich immunoassay technique. To reduce interpatient variability, the percent difference between the Day-0 (non-stimulated, basal) level and the Day-hCG and Day-OVU levels was calculated.


RESULTS
P-selectin level on Day-hCG was significantly higher than on Day-0 (P < 0.05) and non-significantly higher than on Day-OVU (P < 0.12). No significant correlations were observed between serum P-selectin and patient age, amount of gonadotropins used, or estradiol or progesterone level.


CONCLUSIONS
The increase in serum P-selectin level during COH until peak estradiol suggests that COH may potentiate a state of platelet activation which is substantially attenuated after hCG administration.
Department of Obstetrics and Gynecology, Rabin Medical Center, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. orvieto@clalit.org.il
Raoul
R
Orvieto
Walid
W
Badir
Jacob
J
Bar
Antonio
A
La Marca
Jacob
J
Ashkenazi
Ori M
OM
Avrech
Benjamin
B
Fisch
D000328:Adult; D015232:Dinoprostone / Q000097:blood; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D016471:Ovarian Hyperstimulation Syndrome / Q000139:chemically induced / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D019007:P-Selectin / Q000097:blood*; D011374:Progesterone / Q000097:blood
D006062:Gonadotropins; D019007:P-Selectin; D011374:Progesterone; D015232:Dinoprostone