Five hydrophobin genes in Fusarium verticillioides include two required for microconidial chain formation.
Five hydrophobin genes have been identified in the fungal corn pathogen Fusarium verticillioides. HYD1, HYD2, and HYD3 encode Class I hydrophobins. The predicted structures of Hyd1p and Hyd2p are 80% similar, while Hyd3p has an unusually small number of amino acids between the third and fourth cysteines. HYD4 and HYD5 encode Class II hydrophobins. Mutants with HYD1-5 individually deleted and a hyd1deltahyd2delta double mutant were similar to wild-type strains in the amount of disease caused in a corn seedling infection assay and in the number of microconidia produced. Microconidial chains were rare in hyd1delta and hyd2delta mutants as microconidia were present almost exclusively as false heads. Transformation of hyd1delta and hyd2delta mutants with HYD1 and HYD2, respectively, restored microconidial chain formation, but transformation with HYD1::AcGFP and HYD2::AcGFP did not complement the mutation. HYD1::AcGFP and HYD2::AcGFP localized to the outside of conidia in false heads and in chains.
Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany.
Uta
U
Fuchs
Kirk J
KJ
Czymmek
James A
JA
Sweigard
D000595:Amino Acid Sequence; D016384:Consensus Sequence; D020285:Cryoelectron Microscopy; D004271:DNA, Fungal / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology*; D005670:Fusarium / Q000166:cytology / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D017353:Gene Deletion; D005800:Genes, Fungal; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008853:Microscopy; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics* / Q000502:physiology; D016254:Mutagenesis, Insertional; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013172:Spores, Fungal / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
D004271:DNA, Fungal; D005656:Fungal Proteins; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins