Vitellogenin mRNA expression in Cherax quadricarinatus during secondary vitellogenic at first maturation females.
PCR products of 1.1 and 0.9 kb were generated using Cherax quadricarinatus genomic DNA in the first case, and hepatopancreas and ovary cDNAs in the second case. These PCR products were cloned and analyzed for nucleotide sequences. The 1.1 kb fragment was used as a probe for Northern hybridization, revealing a transcript of approximately 8 kb in both tissues. Results from both Northern blot and RT-PCR analyses showed that the mRNA enconding the 3' end of the vitellogenin cDNA was present simultaneously in both hepatopancreas and ovary tissues in secondary vitellogenic at first maturation females, but was not detected in male hepatopancreas. The deduced amino acid sequences of Vitellogenin (Vg) cDNAs from ovary and hepatopancreas confirmed the existence at least two different Vg genes, and two different sites of synthesis.
CIBNOR-Centro de Investigaciones Biológicas del Noroeste, S.C. Programa de Acuacultura, La Paz 23000, B.C.S., México. vserrano04@cibnor.mx
Vania
V
Serrano-Pinto
Igor
I
Landais
Marie-Helene
MH
Ogliastro
Meliza
M
Gutiérrez-Ayala
Humberto
H
Mejía-Ruíz
Humberto
H
Villarreal-Colmenares
Alejandra
A
García-Gasca
Celia
C
Vázquez-Boucard
D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology*; D005260:Female; D043143:Hepatopancreas / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism*
D012333:RNA, Messenger; D014819:Vitellogenins