Optimization of production of conjugated linoleic acid from soybean oil.
Linoleic acid from soybean oil was used to synthesize conjugated linoleic acid (CLA), and the response surface methodology (RSM) was applied to optimize the process. A temperature of -35 degrees C and a solvent to oil sample ratio of 8 were suggested for removal of saturated fatty acids by low-temperature crystallization. The ratio of oil sample/urea/methanol suggested was 1:2:5.5 (w/w/v) for removal of oleic acid by urea crystallization. A temperature of 150 degrees C and a time of 140 min were found to be the optimal conditions in the isomerization for the production of c-9,t-11 and t-10,c-12 CLA isomers.
Yang
Tsung-Shi
TS
Department of Food Science, Chungtai Institute of Health Sciences and Technology, No.11 Pu-tzu lane, Pei-tun District 406, Taichung, Taiwan. tsyang@chtai.ctc.edu.tw
Liu
Tai-Ti
TT
D003080:Cold Temperature; D003460:Crystallization; D005227:Fatty Acids / Q000737:chemistry; D044243:Linoleic Acids, Conjugated / Q000138:chemical synthesis*; D019301:Oleic Acid / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry*; D014508:Urea
D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D019301:Oleic Acid; D013024:Soybean Oil; D014508:Urea