Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa.
FpvA, the pyoverdine outer-membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine-loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 A resolution and a three-wavelength MAD data set was collected at 3.6 A resolution using crystals of selenomethionine-substituted protein. The crystals grew under similar conditions and both belong to space group C2, but have different unit-cell parameters.
Cobessi
David
D
Département Récepteurs et Protéines Membranaires, UPR 9050 CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67410 Illkirch, France. cobessi@esbs.u-strasbg.fr
Célia
Hervé
H
Folschweiller
Nicolas
N
Heymann
Michael
M
Schalk
Isabelle
I
Abdallah
Mohamed
M
Pattus
Franc
F
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D009842:Oligopeptides / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000166:cytology
D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; C042453:pyoverdin