Effects of glucose on fsr-mediated biofilm formation in Enterococcus faecalis.
Biofilm production is frequently dependent on such environmental factors as cell density and glucose concentration. The Enterococcus faecalis quorum-sensing locus (fsr) increases enterococcal virulence in multiple animal models. To date, fsr has been shown to regulate the transcription of 2 downstream protease genes. We demonstrate that the effect of fsr mutations on biofilm formation, as well as the fsr-mediated catabolite control of biofilm, is mediated via these proteases. The present study provides additional insight into the mechanisms used by E. faecalis to establish nosocomial infection.
Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA. spillai@bidmc.harvard.edu
Satish K
SK
Pillai
George
G
Sakoulas
George M
GM
Eliopoulos
Robert C
RC
Moellering
Barbara E
BE
Murray
Roger T
RT
Inouye
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D009154:Mutation
D001426:Bacterial Proteins; C409031:FsrA protein, Enterococcus faecalis; D010450:Endopeptidases; D005947:Glucose