[The first nucleotide of the codon located in the P site of the 80S ribosome is close to G1702 of the 18S rRNA as revealed by crosslinking to a pUUUGUU derivative containing a perfluorophenylazido group at the guanine].
Modification of the 18S rRNA with a pUUUGUU derivative carrying a perfluorophenylazido group at N7 of G was studied in the complex with the human 80S ribosome and Val-tRNA(Val), which directs modified GUU to the P site. Reverse transcription reported modification of invariant G1702 of the 18S rRNA. On evidence of the results and the earlier data on affinity modification of the human ribosome with tetra- or heptaribonucleotide derivatives carrying an alkylating group at the 3' end, the template was assumed to make a bend between the A- and P-site codons, which brings both codons closer to G1702 of the 18S rRNA.
Demeshkina
N A
NA
Repkova
M N
MN
Ven'iaminova
A G
AG
Graĭfer
D M
DM
Karpova
G G
GG
D001483:Base Sequence; D003062:Codon; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D012270:Ribosomes / Q000235:genetics*
D003062:Codon; D012337:RNA, Ribosomal, 18S