Rapid mixing methods for exploring the kinetics of protein folding.
Information on the time-dependence of molecular species is critical for elucidating reaction mechanisms in chemistry and biology. Rapid flow experiments involving turbulent mixing of two or more solutions continue to be the main source of kinetic information on protein folding and other biochemical processes, such as ligand binding and enzymatic reactions. Recent advances in mixer design and detection methods have opened a new window for exploring conformational changes in proteins on the microsecond time scale. These developments have been especially important for exploring early stages of protein folding.
Roder
Heinrich
H
Basic Science Division, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA. roder@fccc.edu
Maki
Kosuke
K
Cheng
Hong
H
Shastry
M C Ramachandra
MC
D000818:Animals; D004867:Equipment Design; D005453:Fluorescence; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D013997:Time Factors
D011506:Proteins