Endotoxin removal from whole blood by a novel adsorption resin: efficiency and hemocompatibility.
The structural component of Gram- bacteria, endotoxin (ET), induces the release of endogenous mediators of sepsis. Attempts to remove these downstream molecules in vivo, have not improved survival. However, extracorporeal strategies such as continuous renal replacement therapy or therapeutic plasmapheresis have shown benefit. We are presenting an affinity-based extracorporeal technology for the removal of ET from whole blood. The small-scale device contains an adsorbent that removed 75% of ET present in whole blood. This affinity resin displayed good hemocompatibility regarding the coagulation pathway. Minimal platelet, neutrophil and complement activation were observed. There was also no evidence of consumption of coagulation factors or cell loss. In as much as ET participates in both the inflammatory and coagulation abnormalities in sepsis, this method represents an efficient and hemocompatible way to remove ET from whole blood, which, in an extracorporeal setting, may improve the outcome of sepsis.
Clarigen Inc., Carlsbad, CA 92008, USA. mcamoureux@clarigen.com
M C
MC
Amoureux
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N
Rajapakse
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E
Hegyi
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D
Le
P
P
Grandics
S
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Szathmary
D000327:Adsorption; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001779:Blood Coagulation Factors / Q000032:analysis; D002452:Cell Count; D002846:Chromatography, Affinity / Q000379:methods; D015926:Complement C3a / Q000032:analysis; D004731:Endotoxins / Q000097:blood*; D005340:Fibrinogen / Q000032:analysis; D016905:Gram-Negative Bacterial Infections / Q000628:therapy; D006487:Hemostasis; D006801:Humans; D019272:Leukocyte Elastase / Q000097:blood; D008024:Ligands; D009000:Monocytes / Q000378:metabolism; D015242:Ofloxacin / Q000494:pharmacology*; D012685:Sepharose; D012772:Shock, Septic / Q000628:therapy; D016212:Transforming Growth Factor beta / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000890:Anti-Infective Agents; D001779:Blood Coagulation Factors; D004731:Endotoxins; D008024:Ligands; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D015926:Complement C3a; D005340:Fibrinogen; D012685:Sepharose; D015242:Ofloxacin; D019272:Leukocyte Elastase