Molecular detection and characterization of Dichelobacter nodosus in ovine footrot in India.
Dichelobacter nodosus was detected in three clinical cases of ovine footrot in Kashmir, India. The detection was done by PCR in three clinical specimens directly, without isolating the organism, using species-specific 16S rDNA primers. Positive results were indicated by amplification of a 783 bp product. All the three samples were subjected to serogrouping by multiplex PCR using group (A-I) specific primers. All the three samples revealed the presence of serogroup B of D. nodosus by yielding a single band of 283 bps.
Wani
S A
SA
Division of Microbiology and Immunology, Faculty of Veterinary Sciences and A.H., S.K. University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190006, India. microlab@sancharnet.in
Samanta
I
I
Bhat
M A
MA
Buchh
A S
AS
D000818:Animals; D020376:Dichelobacter nodosus / Q000235:genetics* / Q000302:isolation & purification*; D005535:Foot Rot / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary*; D007194:India; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012757:Sheep Diseases / Q000382:microbiology*; D034561:Sheep, Domestic / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S