Crystallization and preliminary X-ray diffraction studies of recombinant staphylokinase.
Staphylokinase, a fibrin-specific plasminogen activator, was highly expressed in Escherichia coli and purified by ion-exchange and gel-filtration chromatography. The purified recombinant staphylokinase was fully active and readily crystallized against 1.2 M sodium citrate in 100 mM Tris-HCl buffer at pH 8.0 using the hanging-drop method. Crystals of staphylokinase diffract to better than 2.2 A resolution. The crystal belongs to the tetragonal space group P4(1)2(1)2 or its enantiomorph with unit-cell parameters a = b = 67.5, c = 150.1 A. There are two molecules in the asymmetric unit. In this paper, we described the first crystallization of a kind of plasminogen activator and present the results of preliminary X-ray diffraction data from the native protein.
Zhan
C
C
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
Wan
Z
Z
Chang
W
W
Yue
J
J
Liang
D
D
Tang
Q
Q
Gu
Y
Y
Zhang
X
X
Xu
G
G
Zhu
Y
Y
Song
H
H