Lipopolysaccharides of Bacteroides fragilis, Chlamydia trachomatis and Pseudomonas aeruginosa signal via toll-like receptor 2.
Recognition of bacterial lipopolysaccharide (LPS) is critical in the host defence against Gram-negative infection. While enterobacterial LPS signals via Toll-like receptor 4 (TLR4), it has recently been reported that the LPS of Leptospira interrogans, Legionella pneumophila, Rhizobium species Sin-1 and at least one strain of Porphyromonas gingivalis are capable of signalling via TLR2. Using a TLR transfection assay and measurement of an NF-kappaB-sensitive promoter region, the results show that the LPS of Bacteroides fragilis NCTC-9343, Chlamydia trachomatis LGV-1 and Pseudomonas aeruginosa PAC-611 also signal via TLR2 and it is pointed out that all TLR2-signalling LPS discovered to date demonstrate relatively weak endotoxicity in some models and structural features distinct from those LPS shown to signal via TLR4.
Erridge
Clett
C
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK 2Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, UK.
Pridmore
Alison
A
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK 2Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, UK.
Eley
Adrian
A
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK 2Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, UK.
Stewart
John
J
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK 2Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, UK.
Poxton
Ian R
IR
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK 2Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, UK.
D000222:Adaptation, Physiological; D001441:Bacteroides fragilis / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000737:chemistry / Q000276:immunology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D016952:Legionella pneumophila; D007921:Leptospira interrogans; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism*; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D009000:Monocytes; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology; D016966:Porphyromonas gingivalis; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D012231:Rhizobium; D015398:Signal Transduction; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016209:Interleukin-8; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D008156:Luciferases