Multiple cleavage sites for polymeric immunoglobulin receptor.
Human polymeric immunoglobulin receptor (pIgR) was expressed in baby hamster kidney (BHK) cells using a recombinant vaccinia virus transfection system. Cleavage of pIgR on the cell surface was partially inhibited by the proteinase inhibitor, leupeptin. We addressed the question whether some particular regions of pIgR could affect the efficient cleavage of this molecule, with the following results: (1) a mutant lacking the entire cytoplasmic region resulted in release of secretory component (SC) into the culture supernatant much faster than wild-type; (2) a pIgR mutant lacking the entire extracellular domain 6, the region containing the susceptible cleavage sites, could be cleaved and released as a mutant SC. The transport kinetics of this mutant between endoplasmic reticulum (ER) and Golgi or Golgi and the cell surface was equivalent to wild-type pIgR. Our results indicate that although the main cleavage site is in domain 6, at least one other cleavage site may exist.
Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan. asano-m@dent.nihon-u.ac.jp
Masatake
M
Asano
Nobuko
N
Takenouchi-Ohkubo
Naoyuki
N
Matsumoto
Yoshitaka
Y
Ogura
Hirofumi
H
Nomura
Hisashi
H
Suguro
Itaru
I
Moro
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics / Q000378:metabolism*; D012635:Secretory Component / Q000276:immunology; D013329:Structure-Activity Relationship; D014162:Transfection; D014616:Vaccinia virus
D007976:Leupeptins; D019056:Receptors, Polymeric Immunoglobulin; D012635:Secretory Component; C032854:leupeptin