Role of beta-lactamases and porins in resistance to ertapenem and other beta-lactams in Klebsiella pneumoniae.
High-level resistance to ertapenem was produced by beta-lactamases of groups 1, 2f, and 3 in a strain of Klebsiella pneumoniae deficient in Omp35 and Omp36. From a wild-type strain producing ACT-1 beta-lactamase, ertapenem-resistant mutants for which the ertapenem MICs were up to 128 microg/ml and expression of outer membrane proteins was diminished could be selected.
Jacoby
George A
GA
Lahey Clinic, Burlington, Mssachusetts 01805, USA. george.a.jacoby@lahey.org
Mills
Debra M
DM
Chow
Nancy
N
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D000077727:Ertapenem; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics; D007769:Lactams; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D018272:Porins / Q000502:physiology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000502:physiology*; D047090:beta-Lactams / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D007769:Lactams; D018272:Porins; D047090:beta-Lactams; D001618:beta-Lactamases; D000077727:Ertapenem