In vivo HIV-1 Rev multimerization in the nucleolus and cytoplasm identified by fluorescence resonance energy transfer.
Nuclear export of intron-containing human immunodeficiency virus type 1 RNA is mediated by the viral Rev protein. Rev is a nucleocytoplasmic transport protein that directly binds to its cis-acting Rev-responsive element RNA. Rev function depends on its ability to multimerize. The in vivo dynamics and the subcellular dependence of this process are still largely unexplored. To visualize and quantitatively analyze the mechanism of Rev multimeric assembly in live cells, we used high resolution in vivo fluorescence resonance energy transfer (FRET) and fluorescence recovery after photobleaching. By using two different dynamic FRET approaches (acceptor photobleaching and donor bleaching time measurements), we observed a strong Rev-Rev interaction in the nucleoli of living cells. Most interestingly, we could also detect Rev multimerization in the cytoplasm; however, FRET efficiency in the cytoplasm was significantly lower than in the nucleolus. By using fluorescence recovery after photobleaching, we investigated the mobility of Rev within the nucleolus. Mathematical modeling of the fluorescence recovery after photobleaching recoveries enabled us to extract relative association and dissociation constants and the diffusion coefficient of Rev in the nucleolus. Our results show that Rev multimerizes in the nucleolus of living cells, suggesting an important role of the nucleolus in nucleocytoplasmic transport.
Human Retrovirus Section, Basic Research Laboratory and Image Analysis Laboratory, Science Application International Corporation, NCI-Frederick, Frederick, Maryland 21702-1201, USA.
Dirk
D
Daelemans
Sylvain V
SV
Costes
Edward H
EH
Cho
Rebecca A
RA
Erwin-Cohen
Stephen
S
Lockett
George N
GN
Pavlakis
D002466:Cell Nucleolus / Q000378:metabolism* / Q000821:virology; D003593:Cytoplasm / Q000378:metabolism* / Q000821:virology; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D015694:Gene Products, rev / Q000378:metabolism*; D017930:Genes, Reporter; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D013997:Time Factors; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D054321:rev Gene Products, Human Immunodeficiency Virus