Expression of c-Fos and c-Jun in developing mouse lens.
We carried out immunohistochemistry in embryonic eye-specific antibodies in the mouse to determine the spatial/temporal expression pattern of c-Fos and c-Jun proteins--the main components of the AP-1 transcription factor, in lens epithelial cells during mouse lens morphogenesis. c-Fos protein expression was detected in the equatorial epithelium at E14.5-P25, with a peak at P1-P8. c-Jun protein expression was detected in equatorial lens epithelial cells at E18.5-P8, with a peak at P1. During these intervals, the anterior epithelium lacked expression of c-Fos and c-Jun. They were not detected in an adult mouse lens. The expression pattern of the AP-1 transcription factor in equatorial epithelium may suggest its role in fiber differentiation.
Department of Ophthalmology, Wakayama Medical University, School of Medicine, Kimiidera, Wakayama, Japan. shirai@wakayama-med.ac.jp
Kumi
K
Shirai
Shizuya
S
Saika
Yuka
Y
Okada
Emiko
E
Senba
Yoshitaka
Y
Ohnishi
D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007908:Lens, Crystalline / Q000196:embryology* / Q000254:growth & development; D051379:Mice; D008813:Mice, Inbred ICR; D009024:Morphogenesis; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1