Imaging glucose-regulated insulin secretion and gene expression in single islet beta-cells: control by AMP-activated protein kinase.
The mechanisms by which changes in glucose concentration regulate gene expression and insulin secretion in pancreatic islet beta-cells are only partly understood. Here we describe the development of new technologies for examining these processes at the level of single living beta-cells. We also present recent findings, made using these and other techniques, which implicate a role for adenosine 5'-monophosphate-activated protein kinase in glucose signaling in these cells.
Henry Wellcome Laboratories of Integrated Cell Signalling and Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, United Kingdom. g.a.rutter@bris.ac.uk
Guy A
GA
Rutter
Isabelle
I
Leclerc
Takashi
T
Tsuboi
Gabriela Da Silva
Gda S
Xavier
Frédérique
F
Diraison
Qingwen
Q
Qian
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D007328:Insulin; D049452:Green Fluorescent Proteins; D019869:Phosphatidylinositol 3-Kinases; D055372:AMP-Activated Protein Kinases; D005947:Glucose