Genomic characterization of a Brazilian TT virus isolate closely related to SEN virus-F.
SEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using 'universal' primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38.
Departamento de Virologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Leonardo
L
Diniz-Mendes
Sylvie
S
Devalle
Christian
C
Niel
D000328:Adult; D001938:Brazil; D004266:DNA Virus Infections / Q000821:virology*; D004267:DNA Viruses / Q000145:classification / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D008297:Male; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000235:genetics*
D004279:DNA, Viral