Lack of genotoxicity of formocresol, paramonochlorophenol, and calcium hydroxide on mammalian cells by comet assay.
Formocresol, paramonochlorophenol, and calcium hydroxide are widely used in dentistry because of their antibacterial activities in root canal disinfection. However, the results of genotoxicity studies using these materials are inconsistent in literature. The goal of this study was to examine the genotoxic potential of formocresol, paramonochlorophenol, and calcium hydroxide using mouse lymphoma cells and human fibroblasts cells in vitro by the comet assay. Data were assessed by Kruskal-Wallis nonparametric test. The results showed that all compounds tested did not cause DNA damage for the tail moment or tail intensity parameters. These findings suggest that formocresol, paramonochlorophenol, and calcium hydroxide do not promote DNA damage in mammalian cells and that the comet assay is a suitable tool to investigate genotoxicity.
Ribeiro
Daniel Araki
DA
Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu School of Medicine, Botucatu, SP, Brazil. ak92@hotmail.com
Marques
Mariângela Esther Alencar
ME
Salvadori
Daisy Maria Fávero
DM
D000704:Analysis of Variance; D000818:Animals; D002126:Calcium Hydroxide / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002733:Chlorophenols / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D005566:Formocresols / Q000633:toxicity*; D006801:Humans; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D012388:Root Canal Irrigants / Q000633:toxicity*; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured
D002733:Chlorophenols; D005566:Formocresols; D009153:Mutagens; D012388:Root Canal Irrigants; C031025:formocresol; C029107:4-chlorophenol; D002126:Calcium Hydroxide