Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant Mycobacterium tuberculosis.
The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifampicin-resistant M. tuberculosis strains and showed the inability to detect one of the most frequent mutations, TCG-->TTG mutation in codon 531 of the rpoB gene, which was shown by automated sequencing.
Jelena
J
Tesić
Zorica
Z
Lepsanović
Veljko
V
Mirović
D024881:Drug Resistance, Bacterial; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012293:Rifampin / Q000494:pharmacology*; D017422:Sequence Analysis, DNA
D012293:Rifampin