System beta and system A amino acid transporters in the feline endotheliochorial placenta.
There is no knowledge of the transport mechanisms by which solutes cross the cat placenta or any other endotheliochorial placenta. Here, we investigated whether the amino acid transport systems beta and A are present in the cat placenta using a placental fragment uptake technique. Data were compared with studies in the human placenta, in which the presence of these two transport systems has been well established. A time course of [(3)H]taurine (substrate for system beta) and [(14)C]MeAIB (nonmetabolizable substrate for system A) uptake was determined in the term cat and human placental fragments in the presence and absence (choline substituted) of Na(+), and further studies were carried out over 15 min. Taurine uptake into both cat and human placenta fragments was found to be Na(+) and Cl(-) dependent, and Na(+)-dependent taurine uptake was blocked by excess beta-alanine. MeAIB uptake was found to be Na(+) dependent, and Na(+)-dependent MeAIB uptake was blocked by excess MeAIB or glycine. Western blotting and immunohistochemistry performed on cat and human placenta showed expression of TAUT and ATA2 (SNAT2), proteins associated with system beta and system A activity, respectively. This study therefore provides the first evidence of the presence of amino acid transport systems beta and A in the cat placenta.
Champion
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EE
Academic Unit of Child Health, University of Manchester, St. Mary's Hospital, Hathersage Road, Manchester M13 0JH, UK.
Mann
S J
SJ
Glazier
J D
JD
Jones
C J P
CJ
Rawlings
J M
JM
Sibley
C P
CP
Greenwood
S L
SL
D026905:Amino Acid Transport Systems / Q000145:classification / Q000378:metabolism*; D000818:Animals; D002415:Cats; D002823:Chorion / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D007700:Kinetics; D023421:Models, Animal; D010920:Placenta / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D013654:Taurine / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology
D026905:Amino Acid Transport Systems; D015091:beta-Alanine; C012924:2,2-dimethyl-beta-alanine; D013654:Taurine