Effect of nitric oxide on H+ -efflux in presence of various nutrients in Candida albicans.
In the present study tentative link has been established between H+ -efflux and effect of NO in presence of various nutrients (glucose, 2-deoxy-D-glucose, xylose, proline, glutamic acid and lysine) in C. albicans using sodium nitroprusside (SNP) as a potent source of NO. It was observed that there was a decreasing trend in pH with time, in control, while SNP treated cells showed an initial decline in pH for 10-15 min, followed by an increase in pH up to 30 min. In presence of glucose there was an enhancement in H+ -efflux by 9-fold whereas proline, glutamic acid and lysine showed enhancement by 3, 6 and 1.5-fold respectively. Similar trends in increase in pH after 15 min in SNP treated cells of Candida was observed in presence of all nutrients used. It was demonstrated for the first time that H+ -ATPase of C. albicans was affected by NO.
Enzyme Kinetics and Molecular Physiology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi 110 025, India.
Md Mahfuzul
MM
Haque
Nikhat
N
Manzoor
M Ejaz
ME
Hussain
Luqman A
LA
Khan
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D002176:Candida albicans / Q000187:drug effects* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000378:metabolism / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000737:chemistry; D011522:Protons; D013997:Time Factors
D007477:Ions; D011522:Protons; D009599:Nitroprusside; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases