[Effects of reconstructive human acidic fibroblast growth factor and wild type acidic fibroblast growth factor on skin cell proliferation].
OBJECTIVE
To investigate the effects of reconstructive human acidic fibroblast growth factor (aFGF) and wild type aFGF on skin cell proliferation in rat.


METHODS
Neonatal rat skin (area of 2 mmx2 mm) was cultured in Dulbecco's modification of Eagle's medium containing reconstructive human aFGF and wild type aFGF, respectively. The concentrations of aFGF were 1 microg/L, 10 microg/L, and 100 microg/L. After being cultured for 4 days, the area of skin was measured.


RESULTS
After treatment with two different growth factors in three different concentrations (1 microg/L, 10 microg/L and 100 microg/L) for 4 days, the areas of skin in three reconstructive human aFGF groups were 1.4, 1.5 and 1.3 fold of that of control, respectively and the areas of three wild type aFGF groups were 1.5, 3.2 and 1.6 fold of that of control, respectively, while the area of skin in the control group was (2.96+/-1.12) mm(2). In comparison with those of other groups, the skin area of 10 microg/L wild type aFGF group was significantly increased (P<0.05).


CONCLUSIONS
Reconstructive human aFGF confers less impact on cutaneous cell growth. The capability of wild type aFGF to induce cutaneous cell proliferation is much greater than that of reconstructive human aFGF.
Key Research Laboratory of Wound Repair, Burns Institute, 304th Hospital of PLA, BeiJing 100037, China.
Jing-yu
JY
Zhao
Xiao-bing
XB
Fu
Wei
W
Chen
Tong-zhu
TZ
Sun
Shu-yun
SY
Zheng
Li-xin
LX
Weng
Ying-min
YM
Zhang
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D046509:Tissue Culture Techniques
D016220:Fibroblast Growth Factor 1