Comparative analysis of micro and macro B chromosomes in the Korean field mouse Apodemus peninsulae (Rodentia, Murinae) performed by chromosome microdissection and FISH.
Comparative analysis of micro B and macro B chromosomes of the Korean field mouse Apodemus peninsulae, collected in populations from Siberia and the Russian Far East, was performed with Giemsa, DAPI, Ag-NOR staining and chromosome painting with whole and partial chromosome probes generated by microdissection and DOP-PCR. DNA composition of micro B chromosomes was different from that of macro B chromosomes. All analyzed micro B chromosomes contained clusters of DNA repeats associated with regions characterized by an uncondensed state in mitosis. Giemsa and DAPI staining did not reveal these regions. Their presence in micro B chromosomes led to their special morphology and underestimation in size. DNA repeat clusters homologous to DNA of micro B chromosome arms were also revealed in telomeric regions of some macro B chromosomes of specimens captured in Siberian regions. Neither active NORs nor clusters of ribosomal DNA were found in the uncondensed regions of micro B chromosomes. Possible evolutionary pathways for the origin of macro and micro B chromosomes are discussed.
Rubtsov
N B
NB
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. rubt@bionet.nsc.ru
Karamysheva
T V
TV
Andreenkova
O V
OV
Bochkaerev
M N
MN
Kartavtseva
I V
IV
Roslik
G V
GV
Borissov
Y M
YM
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000737:chemistry* / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007723:Korea; D008297:Male; D042282:Microdissection / Q000379:methods*; D009115:Muridae / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D015342:DNA Probes