Reproductive evaluation of aqueous crude extract of Achillea millefolium L. (Asteraceae) in Wistar rats.
The present study was conducted to evaluate the toxicity of the exposure to the aqueous extract from leaves (AE) of Achillea millefolium L. on reproductive endpoints in Wistar rats. Adult male rats were treated daily with yarrow extract (0.3, 0.6 and 1.2 g/kg/day) during 90 days by oral gavage. Endpoints including reproductive organ weights, sperm and spermatid numbers as well as sperm morphology were evaluated. No clinical signs of toxicity were detected over the treatment period, and body weight gain was similar in all groups. A significant increase in the percentage of abnormal sperm in the group treated with the highest dose of yarrow extract was detected with no other important changes in the other reproductive endpoints studied in the male rats. Furthermore, a possible estrogenic/antiestrogenic activity of the yarrow extract screened after a 3-day treatment of immature female rats which did not show any uterotrophic effects.
Departamento de Farmacologia, Universidade Federal do Paraná, Curitiba/PR, Brazil. pdalsenter@ufpr.br
Paulo R
PR
Dalsenter
Ana M
AM
Cavalcanti
Anderson J M
AJ
Andrade
Samanta L
SL
Araújo
Maria C A
MC
Marques
D031162:Achillea / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D005260:Female; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects / Q000648:ultrastructure; D014599:Uterus / Q000187:drug effects
D004968:Estrogens, Non-Steroidal; D010936:Plant Extracts