Evidence for mitochondrial thioesterase 1 as a peroxisome proliferator-activated receptor-alpha-regulated gene in cardiac and skeletal muscle.
The physiological role of mitochondrial thioesterase 1 (MTE1) is unknown. It was proposed that MTE1 promotes fatty acid (FA) oxidation (FAO) by acting in concert with uncoupling protein (UCP)3. We previously showed that ucp3 is a peroxisome proliferator-activated receptor-alpha (PPAR alpha)-regulated gene, allowing induction when FA availability increases. On the assumption that UCP3 and MTE1 act in partnership to increase FAO, we hypothesized that mte1 is also a PPAR alpha-regulated gene in cardiac and skeletal muscle. Using real-time RT-PCR, we characterized mte1 gene expression in rat heart and soleus muscles. Messenger RNA encoding for mte1 was 3.2-fold higher in heart than in soleus muscle. Cardiac mte1 mRNA exhibited modest diurnal variation, with 1.4-fold higher levels during dark phase. In contrast, skeletal muscle mte1 mRNA remained relatively constant over the course of the day. High-fat feeding, fasting, and streptozotocin-induced diabetes, interventions that increase FA availability, muscle PPAR alpha activity, and muscle FAO rates, increased mte1 mRNA in heart and soleus muscle. Conversely, pressure overload and hypoxia, interventions that decrease cardiac PPAR alpha activity and FAO rates, repressed cardiac mte1 expression. Specific activation of PPAR alpha in vivo through WY-14643 administration rapidly induced mte1 mRNA in cardiac and skeletal muscle. WY-14643 also induced mte1 mRNA in isolated adult rat cardiomyocytes dose dependently. Expression of mte1 was markedly lower in hearts and soleus muscles isolated from PPAR alpha-null mice. Alterations in cardiac and skeletal muscle ucp3 expression mirrored that of mte1 in all models investigated. In conclusion, mte1, like ucp3, is a PPAR alpha-regulated gene in cardiac and skeletal muscle.
Stavinoha
Melissa A
MA
Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 2121 W. Holcombe Blvd., IBT 1011B, Houston, TX 77030, USA.
RaySpellicy
Joseph W
JW
Essop
M Faadiel
MF
Graveleau
Christophe
C
Abel
E Dale
ED
Hart-Sailors
Mary L
ML
Mersmann
Harry J
HJ
Bray
Molly S
MS
Young
Martin E
ME
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D004041:Dietary Fats / Q000378:metabolism; D004195:Disease Models, Animal; D004790:Enzyme Induction / Q000502:physiology; D005215:Fasting / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation; D006321:Heart / Q000187:drug effects; D000860:Hypoxia / Q000378:metabolism; D007473:Ion Channels; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000201:enzymology; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D047493:PPAR alpha / Q000378:metabolism*; D010170:Palmitoyl-CoA Hydrolase / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D020025:Peroxisome Proliferators / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D004041:Dietary Fats; D005227:Fatty Acids; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047493:PPAR alpha; D020025:Peroxisome Proliferators; D011743:Pyrimidines; D012333:RNA, Messenger; C000604393:Ucp3 protein, mouse; C000604394:Ucp3 protein, rat; D000071247:Uncoupling Protein 3; C006253:pirinixic acid; D010170:Palmitoyl-CoA Hydrolase