Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones.
Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT gene following treatment with fungal elicitor, phytohormones, cycloheximide, and inhibitors of protein kinase/phosphatases. SALT expression was up-regulated following treatment with a fungal elicitor, jasmonic acid (JA), abscisic acid (ABA), and NaCl. However, salicylic acid (SA) alone or in combination with one of the other elicitors not only strongly inhibited SALT gene expression but also exhibited an antagonistic effect in suspension cells and leaves. Cycloheximide inhibited SALT accumulation in suspension cells and in leaves, but the inhibitors of protein kinase/phosphatase did not. Immunolocalization revealed that SALT protein was present in xylem parenchyma cells of vascular bundles in the major and minor leaf veins.
Kim
Sun Tae
ST
Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Chinju, Korea. staekim@naver.com
Kim
Sang Gon
SG
Hwang
Du Hyeon
DH
Kang
Sun Young
SY
Koo
Sung Cheol
SC
Cho
Moo Je
MJ
Kang
Kyu Young
KY
D000040:Abscisic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D005819:Genetic Markers / Q000187:drug effects / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology
D003517:Cyclopentanes; D004791:Enzyme Inhibitors; D005819:Genetic Markers; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; C066246:late embryogenesis abundant protein, plant; C076973:SALT protein, Oryza sativa; C011006:jasmonic acid; D000040:Abscisic Acid; D003513:Cycloheximide; D011494:Protein Kinases; D020156:Salicylic Acid