Sensitive determination of domoic acid using high-performance liquid chromatography with electrogenerated tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection.
A new sensitive determination method of domoic acid based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The method exhibited good reproducibility. The relative standard deviation of six replicate measurements was 1.6% for 10 ng ml(-1). A calibration graph, based on a standard domoic acid solution, was linear over the range of 1 - 500 ng ml(-1) (coefficient of correlation, r2 = 0.9995) and the detection limit was 8 pg (signal-to-noise ratio = 3) without any preconcentration and derivatization steps. This new method was successfully applied to a real sample of blue mussels spiked with 2 microg g(-1) domoic acid.
Graduate School of Cultural Studies and Human Science, Kobe University, Tsurukabuto, Nada, Kobe, Japan.
Hitoshi
H
Kodamatani
Keiitsu
K
Saito
Nobumitsu
N
Niina
Shigeo
S
Yamazaki
Akihiko
A
Muromatsu
Ikuo
I
Sakurada
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D017377:Diatoms; D004563:Electrochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D008163:Luminescent Measurements; D008956:Models, Chemical; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012758:Shellfish; D013053:Spectrophotometry; D013997:Time Factors; D014466:Ultraviolet Rays
D009942:Organometallic Compounds; C058901:tris(2,2'-bipyridine)ruthenium III; D015082:2,2'-Dipyridyl; C012301:domoic acid; D007608:Kainic Acid