Analysis of corticosteroids by high performance liquid chromatography-electrospray mass spectrometry.
A high performance liquid chromatography electrospray mass spectrometry (HPLC-ESI-MS) method, for the detection of corticosteroids in cosmetics has been developed. A water-acetonitrile linear gradient on a C-18 reversed-phase column was found to be suitable in separating triamcinolone and its main derivatives, which greatly differ in lipophilicity. Detection was performed in negative electrospray ionisation mode. Good correlation between peaks areas and solutions concentration was found in the range 0.05-10.0 micro g ml(-1) and the detection limits resulted in the range of 20-45 pg injected. The method was successfully applied to the analysis of real samples of shampoo.
Panusa
Alessia
A
Dipartimento di Chimica Fisica ed Inorganica, Universita degli Studi di Bologna, Viale Risorgimento 4, 40136 Bologna, Italy.
Ottaviani
Monica
M
Picardo
Mauro
M
Camera
Emanuela
E
Gagliardi
Luigi
L
Chimenti
Paola
P
Granese
Arianna
A
Tonelli
Domenica
D
D000305:Adrenal Cortex Hormones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000737:chemistry*; D013287:Illicit Drugs / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D000305:Adrenal Cortex Hormones; D003358:Cosmetics; D013287:Illicit Drugs