Change in water temperature on the immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus.
Taiwan abalones, Haliotis diversicolor supertexta, held in 30 parts/per thousand seawater at 28 degrees C, were injected with TSB-grown Vibrio parahaemolyticus (1.6x10(5) cfu abalone(-1)) and then transferred to 20, 24, 28 and 32 degrees C. All abalones transferred to 32 degrees C died by 72 h. The mortality of V. parahaemolyticus-injected abalone held at 20 and 24 degrees C was significantly lower over 24-96 h, compared to animals held at 28 and 32 degrees C. In a separate experiment designed to measure immune function, abalones held in 30 per thousand seawater at 28 degrees C and then transferred to 20, 24, 28 and 32 degrees C were examined for total haemocyte count, phenoloxidase activity, respiratory burst, and phagocytic activity to V. parahaemolyticus after 24, 72 and 120 h. The phenoloxidase activity and phagocytic activity decreased significantly, whereas respiratory burst increased significantly in abalone transferred to 32 degrees C. It is concluded that transfer of abalone from 28 degrees C to 32 degrees C reduced their innate immunity and resistance against V. parahaemolyticus infection.
Cheng
Winton
W
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan, ROC.
Hsiao
I-Shan
IS
Hsu
Chih-Hung
CH
Chen
Jiann-Chu
JC
D000818:Animals; D001772:Blood Cell Count; D004198:Disease Susceptibility / Q000276:immunology*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D016897:Respiratory Burst / Q000276:immunology; D012623:Seawater; D012908:Snails / Q000276:immunology* / Q000382:microbiology*; D013624:Taiwan; D013696:Temperature; D014736:Vibrio parahaemolyticus
D014442:Monophenol Monooxygenase