Enhancement of the antifungal activity of Bacillus subtilis F29-3 by the chitinase encoded by Bacillus circulans chiA gene.
Bacillus subtilis F29-3 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect of chitinase on the antifungal activity of B. subtilis F29-3, a 2.4-kb DNA fragment containing the chiA gene of Bacillus circulans WL-12 was ligated into a shuttle vector pHY300PLK and transformed into B. subtilis F29-3. A bioassay conducted on the culture supernatant showed that, in comparison to the B. subtilis control strain, B. subtilis F29-3 expressing the chiA gene exhibited a greater inhibition of spore germination of Botrytis elliptica, indicating that chitinase could enhance the antifungal function conferred by B. subtilis F29-3.
Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan, R.O.C. cychen@ntu.edu.tw
Chao-Ying
CY
Chen
Yi-Huei
YH
Wang
Chien-Jui
CJ
Huang
D000935:Antifungal Agents / Q000494:pharmacology; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D020171:Botrytis / Q000187:drug effects*; D002688:Chitinases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D005822:Genetic Vectors; D010572:Pest Control, Biological / Q000379:methods; D014170:Transformation, Genetic
D000935:Antifungal Agents; D002688:Chitinases