Generation, maintenance, and adoptive transfer of diabetogenic T-cell lines/clones from the nonobese diabetic mouse.
The ability to generate, maintain, and use cloned lines of T cells reactive for self-antigens has opened up a new avenue of investigation for researchers. These T-cell clones allow the rapid induction of tissue-specific autoimmunity with the intent of dissecting the contribution of the different cell types involved. T cells from the diabetes-prone nonobese diabetic mouse are proving to be a vital asset for understanding the T-cell-mediated pathogenesis that leads to overt beta-cell destruction. T-cell clone adoptive transfer protocols have been developed for use in immunodeficient strains, thus reducing the complexity of mechanism of disease initiation. Furthermore, these T-cell clones have been used to derive T-cell receptor transgenic (TCR-Tg) animals carrying only self-reactive T cells. The use of these TCR-Tg animals to study pathogenesis has also evolved from the ability to generate, maintain, and use T-cell cloned lines. This chapter focuses on primary culture for the generation of T-cell lines and clones, their long-term maintenance, and their use in disease transfer for studying the pathogenesis of end-organ autoimmunity.
Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh, PA, USA.
Martha J
MJ
Milton
Michelle
M
Poulin
Clayton
C
Mathews
Jon D
JD
Piganelli
D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001324:Autoantigens; D018929:Cell Culture Techniques; D002460:Cell Line; D002999:Clone Cells; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology* / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens; D005947:Glucose