Reservoir of Candida albicans infection in a vascular bypass graft demonstrates a stable karyotype over six months.
We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.
Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA.
Paul
P
Lephart
Patricia
P
Ferrieri
Jo-Anne
JA
van Burik
D001769:Blood / Q000382:microbiology; D001807:Blood Vessel Prosthesis / Q000382:microbiology*; D002176:Candida albicans / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D016469:Fungemia; D005838:Genotype; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009055:Mouth / Q000382:microbiology; D016533:Mycological Typing Techniques; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D012007:Rectum / Q000382:microbiology; D012189:Retrospective Studies
D004271:DNA, Fungal