Structural and energetic consequences of expanding a highly cooperative stable DNA hairpin loop.
Many hairpin loops are expanded versions of smaller, stable ones. Herein we investigate the extent to which the energetics and structure of d(cGNAg) hairpin loops will tolerate sequence variation. Changing the closing base pair from CG to GC was found to completely eliminate loop-loop interactions; in contrast, expanding the loop at the 3'-end resulted in similar energetics and nonadditivity parameters as the parent loop, suggesting that loop-loop interactions remain intact and highly coupled upon expansion. Together, these data suggest that the CG closing base pair forms an essential platform upon which a stable d(GNA) hairpin loop can fold and that this loop can undergo 3'-expansion with little effect to its structure or energetics.
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Ellen M
EM
Moody
Philip C
PC
Bevilacqua
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D011685:Purine Nucleotides / Q000737:chemistry*; D013816:Thermodynamics
D011685:Purine Nucleotides; D004247:DNA