Binding interactions of Escherichia coli with globotetraosylceramide (globoside) using a surface plasmon resonance biosensor.
Surface plasmon resonance with an alkane L1 chip was used to investigate the binding of uropathogenic Escherichia coli, carrying adhesion receptors, to globotetraosylceramide (globoside; GbO4). The immobilization of globoside was reproducible and resulted in a stable globoside layer on the L1 chip. The data indicated that the globoside-immobilized L1 chip could be used for studying interactions with live or chemically fixed E. coli. The results indicated that the dissociation time was significantly reduced in glutaraldehyde-fixed E. coli as compared to living cells. Overall, the report demonstrates the significance of the L1 chip in terms of sensitivity, specificity, handling, and speed when studying globoside/E. coli interactions. This model may assist in screening for compounds that can inhibit the binding of uropathogenic E. coli to glycolipid ligands on target cells.
Department of Chemistry and Biomedical Sciences, University of Kalmar, Norra vagen 49, Kalmar SE-391 82, Sweden.
Mohammad R
MR
Pourshafie
Britt-Inger
BI
Marklund
Sten
S
Ohlson
D001422:Bacterial Adhesion / Q000502:physiology; D015374:Biosensing Techniques / Q000379:methods; D004912:Erythrocytes / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism*; D005915:Globosides / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D005915:Globosides; C024032:globotetraosylceramide