H(2) production and carbon utilization by Thermotoga neapolitana under anaerobic and microaerobic growth conditions.
H(2) production by Petrotoga miotherma, Thermosipho africanus, Thermotoga elfii, Fervidobacterium pennavorans, and Thermotoga neapolitana was compared under microaerobic conditions. Contrary to these previously reported strains being strict anaerobes, all tested strains grew and produced H(2) in the presence of micromolar levels of O(2). T. neapolitana showed the highest H(2) production under these conditions. Microscopic counting techniques were used to determine growth curves and doubling times, which were subsequently correlated with optical density measurements. The Biolog anaerobic microtiter plate system was used to analyze the carbon source utilization spectrum of T. neapolitana and to select non-metabolized or poorly metabolized carbohydrates as physiological buffers. Itaconic acid was successfully used as a buffer to overcome pH-induced limitations of cell growth and to facilitate enhanced production of CO-free H(2).
National Technology Energy Laboratory, 3610 Collins Ferry Rd., Morgantown, WV 26507, USA.
Suellen A
SA
Van Ooteghem
Amy
A
Jones
Daniel
D
Van Der Lelie
Bin
B
Dong
Devinder
D
Mahajan
D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism*; D013045:Species Specificity; D046949:Thermotoga neapolitana / Q000254:growth & development* / Q000378:metabolism*
D002244:Carbon; D006859:Hydrogen; D010100:Oxygen