Nisin-controlled production of pediocin PA-1 and colicin V in nisin- and non-nisin-producing Lactococcus lactis strains.
The introduction of chimeric genes encoding the fusion leader of lactococcin A-propediocin PA-1 or procolicin V under the control of the inducible nisA promoter and the lactococcin A-dedicated secretion genes (lcnCD) into Lactococcus lactis strains, including a nisin producer, expressing the two component regulator NisRK led to the production or pediocin PA-1 or colicin V, respectively.
Food Safety Science Division, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich, UK.
Nikki
N
Horn
Antonio
A
Fernández
Helen M
HM
Dodd
Michael J
MJ
Gasson
Juan M
JM
Rodríguez
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis*; D003087:Colicins / Q000096:biosynthesis*; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009561:Nisin / Q000378:metabolism*; D000070017:Pediocins
D001426:Bacterial Proteins; D001430:Bacteriocins; D003087:Colicins; D003470:Culture Media; D000070017:Pediocins; C074288:pediocin PA-1; D009561:Nisin