Robustness of the extraction step when parallel factor analysis (PARAFAC) is used to quantify sulfonamides in kidney by high performance liquid chromatography-diode array detection (HPLC-DAD).
The robustness of a multiresidue method has been analysed for the extraction and quantification of sulfamethoxypyridazine, sulfamethoxazole and sulfadimethoxine in porcine kidney by HPLC-DAD through a Plackett-Burman design. Two experimental responses were examined, the mean recovery from three replicates (accuracy) and their standard deviation (precision). Three factors were tested: the volume of phosphoric acid (pH) added in the extraction step, the time used for passing the sample through the solid-phase extraction cartridge (flow rate) and methanol volume to elute the analytes from the cartridge. Due to the non-specificity of the chromatograms (unknown matrix interferences coelute with each sulfonamide) the PARAFAC model was employed to evaluate the concentration recovered in the experiments of the Plackett-Burman design as well as to identify the spectra of the substances according to the criteria set in the European Decision 2002/657/EC for the analysis of residues. The extraction step was concluded to be robust to the recovery and the standard deviation of all three analytes.
Department of Chemistry, Faculty of Sciences, University of Burgos, Pza. Misael Banuelos s/n, 09001 Burgos, Spain.
Inmaculada
I
García
Luis
L
Sarabia
M Cruz
MC
Ortiz
J Manuel
JM
Aldama
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005163:Factor Analysis, Statistical; D007668:Kidney / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
D013449:Sulfonamides