Establishment and characterization of two cell lines (HEC-155, HEC-180) derived from uterine papillary serous adenocarcinoma.
Uterine papillary serous adenocarcinoma (UPSC) is an uncommon histologic subtype of endometrial cancer that characteristically behaves aggressively with a poor prognosis. We established two novel cell lines derived from UPSC designated HEC-155 and HEC-180. Both cell lines have been growing steadily in monolayer cultures for over ten years. Overexpression of p53, Ki67 and p27 was detected in both cell lines by immunohistochemistry. Using a DNA sequencing technique, a point mutation of p53 was detected in exon 8, codon 286 in HEC-155 and in exon 6, codon 195 in HEC-180. These newly established cell lines should be useful for investigating the characteristics of UPSC.
Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.
T
T
Iida
M
M
Hamano
N
N
Yoshida
K
K
Yonamine
K
K
Hayashi
K
K
Kiguchi
B
B
Ishizuka
Y
Y
Nishimura
T
T
Arai
M
M
Kawaguchi
H
H
Hata
J
J
Watanabe
H
H
Kuramoto
D001707:Biopsy, Needle; D002455:Cell Division / Q000502:physiology; D045744:Cell Line, Tumor; D018283:Cystadenocarcinoma, Papillary / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008875:Middle Aged; D009154:Mutation; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology*
D016159:Tumor Suppressor Protein p53