TGF-beta1 neutralizing antibodies decrease the fibrotic effects of ischemic priapism.
The objective of this study was to evaluate the possible role of transforming growth factor beta 1 (TGF-beta1) antibodies (ab) for the prevention of fibrotic effects of priapism in a rat model. In total, 30 adult Sprague-Dawley rats were divided into five groups. Priapism with 6 h (group 1), priapism with 6 h+ab (group 2), priapism with 24 h (group 3), priapism with 24 h+ab (group 4) and control (group 5). Priapism was induced with a vacuum erection device and a rubber band was placed at the base of the erect penis. At 1 h after the initiation of priapism, TGF-beta1 antibodies were given intracavernosaly. All rats underwent electrical stimulation of the cavernous nerve after 8 weeks. Intracavernous and systemic blood pressures were measured during the procedure. Rats in group 1 showed significantly higher (intracavernosal pressure (ICP) pressures to cavernous nerve stimulation and had higher ICP/BP ratios when compared to other groups. Similarly, histopathologic examination revealed less fibrosis in group 2, compared with the other groups. Consequently, TGF-beta1 antibodies antagonise the fibrotic effects of TGF-beta1, especially in cases with duration of priapism less than 6 h.
Sanli
O
O
Istanbul Faculty of Medicine, University of Istanbul, Istanbul, Turkey.
Armagan
A
A
Kandirali
E
E
Ozerman
B
B
Ahmedov
I
I
Solakoglu
S
S
Nurten
A
A
Tunç
M
M
Uysal
V
V
Kadioglu
A
A
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D001794:Blood Pressure; D004558:Electric Stimulation; D005355:Fibrosis / Q000517:prevention & control; D007511:Ischemia; D008297:Male; D010413:Penis / Q000098:blood supply* / Q000294:innervation / Q000473:pathology*; D011317:Priapism / Q000150:complications* / Q000503:physiopathology / Q000628:therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000276:immunology*; D053773:Transforming Growth Factor beta1
D000906:Antibodies; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1