Proteomic analysis of post-mitochondrial fractions of young and old rat kidney.
Proteomic analysis is defined as the characterization of the entire set of proteins encoded by a genome. Two-dimensional (2D) electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) are key technologies used in proteomic analysis to gain information about protein expression profiles and post-translational modifications. Knowledge about aging processes can be gained by recognizing changes in protein expression. Thus, to better understand the aging process through protein profiling, post-mitochondrial (PM) fractions of young (13-month) and old (31-month) male Fischer 344 rat kidney were differentially analyzed by 2D. We detected a total number of 380 spots on 2D gel images. Among them, 167 spots showed 2-fold significant alterations (p<0.05) between young and old PM fractions. Further, 103 proteins were identified by MALDI-TOF MS. The PM fraction of aged rat kidney showed increases in antioxidative and proteolytic proteins and decreases in cytoskeletal proteins. In addition, we found age-related changes in transport and homeostasis proteins. Thus, our results demonstrated that proteomic analysis can be effectively applied to the assessment of the age status of protein expression, and thereby provide valuable information on age-related changes of proteome.
Interdisciplinary Research Program of Bioinformatics, Pusan National University, Gumjung-ku, Busan 609-735, South Korea.
Chul Hong
CH
Kim
Dae Ui
DU
Park
An Sung
AS
Chung
Yani
Y
Zou
Kyung Jin
KJ
Jung
Bo Kyung
BK
Sung
Byung Pal
BP
Yu
Hae Young
HY
Chung
D000375:Aging / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D003598:Cytoskeletal Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D006487:Hemostasis; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D051381:Rats; D011916:Rats, Inbred F344; D013047:Specific Pathogen-Free Organisms; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003598:Cytoskeletal Proteins; D011506:Proteins