Synthesis and evaluation of novel bioactive composite starch/bioactive glass microparticles.
The aim of the development of composite materials is to combine the most desired properties of two or more materials. In this work, the biodegradable character, good controlled-release properties, and natural origin of starch-based biomaterials are combined with the bioactive and bone-bonding properties of bioactive glass (BG). Novel, bioactive composite starch-BG microparticles were synthesized starting from a blend of starch and polylactic acid (50%/50% wt) with BG 45S5 powder using a simple emulsion method. Morphological and chemical characterization showed that these particles exhibited a spherical morphology with sizes up to 350 microm and that BG 45S5 was incorporated successfully into the composite particles. Upon immersion in a solution simulating body fluids, for periods up to 3 weeks, their bioactive nature was confirmed, as a calcium-phosphate layer resembling biological apatite was formed onto their surface. The short-term cytotoxicity of these materials was also tested by placing 24-h leachables of the materials extracted in culture medium in contact with a fibroblastic cell line (L929) up to 72 h. At this time period, two biochemical tests--MTT and total protein quantification--were performed. The results showed that these materials are not cytotoxic. These results constitute the basis of future encapsulation studies using bone-acting therapeutic agents such as bone morphogenetic proteins or other bone-relevant factors. The particles developed here may be very useful for applications in which controlled release, degradability, and bone-bonding ability are the main requirements.
Silva
G A
GA
3B's Research Group, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. gsilva@dep.uminho.pt
Costa
F J
FJ
Coutinho
O P
OP
Radin
S
S
Ducheyne
P
P
Reis
R L
RL
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002516:Ceramics; D005898:Glass / Q000737:chemistry; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D010316:Particle Size; D010758:Phosphorus / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D012825:Silicon / Q000378:metabolism; D013213:Starch / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D013499:Surface Properties; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C425330:bioactive glass 45S5; D010758:Phosphorus; D019344:Lactic Acid; C033616:poly(lactide); D013213:Starch; D002118:Calcium; D012825:Silicon