Poly-N-acetyl glucosamine-mediated red blood cell interactions.
BACKGROUND
Investigations were performed to assess the effect of poly-N-acetyl glucosamine (p-GlcNAc) fiber slurry-mediated hemostasis by interactions with red blood cells.


METHODS
Red blood cell aggregation studies were performed using test material-coated microscope slides and multiphoton microscopic measurements. Enzymatic removal of red blood cell surface proteins was achieved using trypsin and neuraminidase treatment. Zeta-potential measurements (surface charge) were performed.


RESULTS
Red blood cells interact directly with poly-N-acetyl glucosamine polymers through ionic interactions and cell-surface proteins. The effective concentration of poly-N-acetyl glucosamine fiber material for 50% red blood cell aggregation was 0.28 mg/mL. The p-GlcNAc beta-configuration fibers and an alpha-configuration structural modification of the fibers both produced maximal responses because of their zeta-potentials, whereas other chemically modified p-GlcNAcs and chitosans were ineffective.


CONCLUSIONS
Poly-N-acetyl glucosamine-induced red blood cell aggregation is mediated by interactions with red blood cell surface charges.
Thatte
Hemant S
HS
Department Surgery, VA Boston Healthcare System, Brigham and Women's Hospital, Harvard Medical Schools, Boston, MA, USA. hemant_thatte@hms.harvard.edu
Zagarins
Sofija E
SE
Amiji
Mansoor
M
Khuri
Shukri F
SF
D000117:Acetylglucosamine / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan; D004353:Drug Evaluation, Preclinical; D004586:Electrophoresis; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006400:Hematocrit; D006488:Hemostasis, Surgical / Q000379:methods; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007477:Ions; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects; D036641:Microscopy, Fluorescence, Multiphoton; D055095:Optics and Photonics; D010973:Platelet Adhesiveness / Q000187:drug effects; D012995:Solubility; D013053:Spectrophotometry; D013499:Surface Properties / Q000187:drug effects
D006490:Hemostatics; D007477:Ions; D008565:Membrane Proteins; C113831:poly-N-acetyl glucosamine; D002686:Chitin; D048271:Chitosan; D000117:Acetylglucosamine