A structure-activity study of the neuropeptide PF1, SDPNFLRFamide, using the dorsal body wall muscle of the chicken nematode, Ascaridia galli.
The action of a range of N terminally modified peptides structurally related to the nematode peptide PF1, SDPNFLRFamide, has been investigated using a dorsal muscle strip preparation from the chicken nematode, Ascaridia galli. Acetylcholine contracts this muscle preparation in a concentration-dependent manner when applied in the range 1-100 microM with an EC50 value of 9 microM. These contractions are reduced in the presence of PF1 and its analogues, with a threshold effect of PF1 of around 1 nM and an IC50 value of 470 nM against 10 microM acetylcholine. All the PF1 analogues tested were less potent than PF1 in reducing the acetylcholine contractions, indicating the importance of the N terminal amino acids in the action of PF1 in this preparation.
School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK.
C J
CJ
Franks
R J
RJ
Walker
Lindy
L
Holden-Dye
D000109:Acetylcholine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001197:Ascaridia; D002645:Chickens / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D019835:FMRFamide / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D009132:Muscles / Q000378:metabolism*; D009479:Neuropeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship; D013997:Time Factors
D009479:Neuropeptides; D010455:Peptides; C086748:SDPNFLRFamide; D019835:FMRFamide; D000109:Acetylcholine