Facile synthesis of membrane-embedded peptides utilizing lipid bilayer-assisted chemical ligation.
Lipid bilayer-assisted chemical ligation between thiolester and N-terminal cysteine peptides has been developed with successful application to the synthesis of membrane protein segments possessing both two transmembrane and one extracellular regions.
Otaka
Akira
A
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. aotaka@pharm.kyoto-u.ac.jp
Ueda
Satoshi
S
Tomita
Kenji
K
Yano
Yoshiaki
Y
Tamamura
Hirokazu
H
Matsuzaki
Katsumi
K
Fujii
Nobutaka
N
D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism
D004952:Esters; D008024:Ligands; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D013438:Sulfhydryl Compounds; D003545:Cysteine