Promoter variant-dependent expression of the STAT5A gene in bovine liver.
Using PCR-heteroduplex and sequencing we identified A/G substitution at position -488 in the promoter region of the bovine STAT5A gene. With reverse transcription-PCR (RT-PCR) and other means we showed that the STAT5A expression level in the liver was higher in cattle with AA than GG genotype. Electrophoretic mobility shift assay (EMSA) showed that the A-->G transition at position -488 increased the STAT5A gene promoter binding capacity for liver nuclear proteins [possibly hepatic nuclear factor (HNF)-3].
Department of Molecular Biology, Institute of Genetics and Animal Breeding Polish Academy of Sciences, Postepu 1, Jastrzebiec, 05-552 Wólka Kosowska, Poland. kflisikowski@yahoo.co.uk
Krzysztof
K
Flisikowski
Rafał R
RR
Starzyński
Lech
L
Zwierzchowski
D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D051541:Hepatocyte Nuclear Factor 3-alpha; D008099:Liver / Q000378:metabolism*; D008297:Male; D008894:Milk Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
D004268:DNA-Binding Proteins; D051541:Hepatocyte Nuclear Factor 3-alpha; D008894:Milk Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors