Comparison of yeast (Candida maltosa) and bacterial (Rhodococcus erythropolis) phenol hydroxylase activity and its properties in the phenolic compounds biodegradation.
Aromatic contaminants of the environment, to which belongs phenol and its derivatives, are toxic and in most of the cases hard to degrade. Removal of these pollutants by biological, gentle and effective way, depends on specific environmental conditions in the locality and on the biodegradation potential of the used microbial population. Closer characterization of the biodegradation and enzyme mechanisms is therefore an essential assumption of the successful implementation of microbes. This paper is focused on comparison of the biodegradation activity between the soil yeast Candida maltosa and bacteria Rhodococcus erythropolis towards various aromatics connected with determination of the first enzyme of the phenol biodegradation pathway: phenol hydroxylase (PH). The effect of substrate type, substrate concentration, growth phase of the microorganisms and presence of humic acids in the cultivation medium, on phenol biodegradation and PH activity are discussed.
Fialová
A
A
Institute of Chemical Technology in Prague, Department of Fermentation Chemistry and Bioengineering, Technická 5, CZ-166 28, Prague 6, Czech Republic,. Ariana.Fialova@vscht.cz
Cejková
A
A
Masák
J
J
Jirků
V
V
D000393:Air Pollutants; D001673:Biodegradation, Environmental; D002175:Candida / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010636:Phenols / Q000493:pharmacokinetics*; D012240:Rhodococcus / Q000201:enzymology* / Q000254:growth & development; D018505:Waste Management / Q000379:methods
D000393:Air Pollutants; D010636:Phenols; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase