Telomerase-immortalized lymphatic and blood vessel endothelial cells are functionally stable and retain their lineage specificity.
OBJECTIVE
Microvasculature plays an important role in a variety of physiological and pathological processes. The authors have previously shown that primary cultures of human microvascular endothelial cells consist of 2 distinct populations of blood vascular and lymphatic endothelial cells. These subtypes are ephemeral and lose purity through passaging. To generate reproducible in vitro and in vivo experiments stable blood and lymphatic endothelial cell lines are an essential prerequisite.


METHODS
In this study they have used human telomerase gene-immortalized nontransformed human microvascular endothelial cell cloned pure cultures of blood and lymphatic endothelial cell subpopulations. Flow cytometry, immunofluorescence, Northern and Western blotting, microarray gene analysis, as well as basic functional assays were used to characterize these clones.


CONCLUSIONS
Immortalized blood and lymphatic subpopulations are stable and functionally specialized cell lineages that expressed pan-endothelial and cell-type-specific markers. They are excellent candidates for long-term culture studies on microvascular-related diseases.
Department of Surgery, University of Vienna, Vienna, Austria.
Sebastian F
SF
Schoppmann
Afschin
A
Soleiman
Romana
R
Kalt
Yukari
Y
Okubo
Christian
C
Benisch
Usha
U
Nagavarapu
G Scott
GS
Herron
Silvana
S
Geleff
D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D019070:Cell Lineage; D002999:Clone Cells / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology; D004729:Endothelium, Lymphatic / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D019098:Telomerase / Q000235:genetics / Q000502:physiology*
D015415:Biomarkers; D019098:Telomerase