Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2.
Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation.
Department of Clinical Oral Molecular Biology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Higashiku, Fukuoka, Japan.
K
K
Iohara
M
M
Nakashima
M
M
Ito
M
M
Ishikawa
A
A
Nakasima
A
A
Akamine
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003785:Dental Pulp Capping / Q000379:methods; D003786:Dental Pulp Cavity / Q000601:surgery; D003804:Dentin / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D003810:Dentinogenesis / Q000187:drug effects / Q000502:physiology; D004285:Dogs; D006801:Humans; D009804:Odontoblasts / Q000187:drug effects; D011994:Recombinant Proteins; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013552:Swine; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2