Supramolecular pellicle precursors.
Saliva contacting with solid surfaces in the oral cavity forms a coat termed the pellicle. However, its formation is not fully understood. Although indications for the existence of supramolecular pellicle precursors have been reported, the possible relationship between them and pellicle formation is unclear. This study investigates the ability of supramolecular precursors to form the pellicle via interaction with a solid surface. Fixed and unfixed salivary globes were spread onto a microscopic grid and examined by transmission electron microscopy. Biochemical pretreatment of saliva revealed that neither disulphide links nor transglutaminase-mediated crosslinking are responsible for maintaining the salivary globes, i.e. supramolecular pellicle precursors. However, the detergent, sodium dodecyl sulphate, caused dissociation of the salivary globes, indicating their micellar nature. Saliva contacting a formvar film for 10 s did not form a complete surface coating, but single supramolecular pellicle precursors were observed attached to the surface. After extension of the contact time to 60 s, a surface layer was formed by clustering and fusion of the supramolecular pellicle precursors. The supramolecular pellicle precursors are unstable and attain a thermodynamically more favourable state by adhesion to a solid surface. As a result, a layer of fused precursors covering the solid surface is formed -- the salivary pellicle.
Vitkov
Ljubomir
L
Department of Electron Microscopy, Light Microscopy and Digital Image Acquisition, University of Salzburg, Salzburg, Austria.
Hannig
Matthias
M
Nekrashevych
Yuriy
Y
Krautgartner
Wolf Dietrich
WD
D044622:Dental Pellicle / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D008823:Micelles; D008854:Microscopy, Electron; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors; D012471:Salivary Proteins and Peptides / Q000737:chemistry; D000267:Tissue Adhesions
D046911:Macromolecular Substances; D008823:Micelles; D010455:Peptides; D011498:Protein Precursors; D012471:Salivary Proteins and Peptides