Rv2686c-Rv2687c-Rv2688c, an ABC fluoroquinolone efflux pump in Mycobacterium tuberculosis.
The Mycobacterium tuberculosis Rv2686c-Rv2687c-Rv2688c operon, encoding an ABC transporter, conferred resistance to ciprofloxacin and, to a lesser extent, norfloxacin, moxifloxacin, and sparfloxacin to Mycobacterium smegmatis. The resistance level decreased in the presence of the efflux pump inhibitors reserpine, carbonyl cyanide m-chlorophenylhydrazone, and verapamil. Energy-dependent efflux of ciprofloxacin from M. smegmatis cells containing the Rv2686c-Rv2687c-Rv2688c operon was observed.
Department of Genetics and Microbiology, University of Pavia, 27100 Pavia, Italy.
Maria Rosalia
MR
Pasca
Paola
P
Guglierame
Fabio
F
Arcesi
Marco
M
Bellinzoni
Edda
E
De Rossi
Giovanna
G
Riccardi
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D017931:DNA Primers; D004351:Drug Resistance; D004734:Energy Metabolism / Q000187:drug effects; D024841:Fluoroquinolones / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D006835:Hydrazones / Q000494:pharmacology; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism*; D009876:Operon / Q000235:genetics; D012110:Reserpine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D018528:ATP-Binding Cassette Transporters; D000890:Anti-Infective Agents; D002121:Calcium Channel Blockers; D017931:DNA Primers; D024841:Fluoroquinolones; D006835:Hydrazones; C070053:carbonyl 3-chlorophenylhydrazone; D002939:Ciprofloxacin; D012110:Reserpine; D014700:Verapamil