Effects of oxidized low-density lipoprotein on leukocyte-endothelial interactions in the rat mesenteric microcirculation during pregnancy.
OBJECTIVE
The accumulation of evidence implicates oxidized lipoproteins in atherosclerosis. Treatment of endothelial cells with these lipoproteins stimulates monocyte binding and the production of chemotactic factors that contribute to inflammation and endothelial injury and dysfunction. In preeclampsia, circulating low-density lipoprotein particles, which are susceptible to oxidation, are increased. We studied leukocyte-endothelial interactions that were related to oxidized lipoproteins in pregnant rats.


METHODS
We examined oxidized low-density lipoprotein-induced leukocyte behavior and uptake of fluorescent-labeled oxidized low-density lipoprotein in rat mesenteric venules during pregnancy, with the use of intravital microscopy with a video imager.


RESULTS
The administration of oxidized low-density lipoprotein significantly reduced rolling velocities of leukocytes in venules and increased the numbers of leukocytes that adhered to endothelium in both nonpregnant and pregnant rats. These interactions were attenuated in pregnancy, when uptake of labeled oxidized low-density lipoprotein into leukocytes and endothelial cells also was decreased.


CONCLUSIONS
Pregnancy may be associated with antioxidant effects.
Department of Obstetrics and Gynecology, Ehime University School of Medicine, Shigenobu, Japan. tkatayam@m.ehime-u.ac.jp
Tomihiro
T
Katayama
Akari
A
Tanaka-Shiraishi
Masaki
M
Kiyomura
Takashi
T
Matsumoto
Yasuki
Y
Kusanagi
Masaharu
M
Ito
D000818:Animals; D001161:Arteriosclerosis / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D005260:Female; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000502:physiology
D008077:Lipoproteins, LDL