Acetylcholine-induced translocation of RhoA in freshly isolated single smooth muscle cells of rat bronchi.
By using immunofluorostaining and confocal laser microscopy, acetylcholine-induced translocation of RhoA was visualized in freshly isolated bronchial smooth muscle cells of the rat. The cellular distribution of RhoA at rest was observed uniformly in the cytosolic space with no staining in the nucleus, whereas acetylcholine stimulation induced a relocalization of RhoA to the cell periphery. From the results of line scans and surface plots, the peripheral to cytosolic ratio of RhoA was significantly increased by acetylcholine stimulation. Thus, the present study clearly demonstrated an acetylcholine-induced translocation of RhoA to the plasma membrane in single bronchial smooth muscle cells of the rat.
Chiba
Yoshihiko
Y
Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo 142-8501, Japan. chiba@hoshi.ac.jp
Uchida
Tetsuro
T
Sakai
Hiroyasu
H
Oku
Teruaki
T
Itoh
Saotomo
S
Tsuji
Tsutomu
T
Misawa
Miwa
M
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009119:Muscle Contraction / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D020742:rhoA GTP-Binding Protein / Q000187:drug effects* / Q000378:metabolism
D020742:rhoA GTP-Binding Protein; D000109:Acetylcholine