A survey of Leishmania braziliensis genome by shotgun sequencing.
We have carried out a survey of the genome of Leishmania (Viannia) braziliensis by shotgun sequencing. Approximately 15% of the haploid genome of the parasite (5.15 Mb of genomic sequence) was obtained. A large number of known and putative genes, predicted to be involved in several cellular processes, were identified. Some genomic features were investigated, such as the general G + C content, which was found to be lower than L. major (57% versus 63%). BlastN searches revealed that 60.2% of the clusterized GSS sequences displayed similarity to L. major genomic sequences, while a BlastX search showed that 45.3% of the thus obtained predicted protein sequences showed similarity to annotated proteins of L. major. Further comparison of the degree of conservation between L. major and L. braziliensis revealed that coding regions are much more conserved than non-coding ones. The shotgun sequence analysis of Leishmania braziliensis appears to be an efficient and suitable strategy contributing to the search for vaccines and novel drug targets. The sequence data described in this paper have been submitted to the dbGSS database under the following accession numbers (BX530413 to BX530454; BX530456 to BX530718; BX538354 to BX539305; BX539350 to BX540325; BX541002 to BX544869; BX544893 to BX545685; BX897701 to BX897710; BX905184 to BX907797; BX907798 to BX908381; BX908403 to BX908718). All data including sequences are also available at (www.ebi.ac.uk/embl/).
Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, 14049-900, Brasil.
Eliane C
EC
Laurentino
Jeronimo C
JC
Ruiz
Gholam
G
Fazelinia
Peter J
PJ
Myler
Wim
W
Degrave
Marcelo
M
Alves-Ferreira
José Marcos C
JM
Ribeiro
Angela K
AK
Cruz
D000818:Animals; D001482:Base Composition; D017124:Conserved Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D007892:Leishmania braziliensis / Q000235:genetics*; D018320:Leishmania major / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D016054:DNA, Protozoan