Capillary electrophoresis of biological particles: viruses, bacteria, and eukaryotic cells.
A review about the application of electrophoretic methods in the capillary format for the investigation of large biological assemblies like viruses, bacteria, yeast or entire mammalian cells is given. These entities are of a size ranging between some nanometers and tens of micrometers. They can form colloidal solutions or dispersions and move under the influence of an electric field. They are separated by zone electrophoresis according to their different electrophoretic velocity, and characterized by the electrophoretic mobility, which is easily determinable in free solution in capillaries or in other microdevices. As the charge of these particles, when being amphoteric, is pH-dependent, isoelectric focusing can also be carried out and the capillary format is increasingly being employed for their separation and determination of pI values. Furthermore, interactions with ligands can be assessed by various modes of affinity capillary electrophoresis. Capillary zone electrophoresis has thus become a valuable tool for investigation of large macromolecular assemblies in the field of biochemistry, clinical chemistry, toxicology, and nutrition chemistry amongst many others.
Kremser
Leopold
L
Institute for Analytical Chemistry, University of Vienna, Vienna, Austria. kremser@anc.univie.ac.at
Blaas
Dieter
D
Kenndler
Ernst
E
D001419:Bacteria / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005057:Eukaryotic Cells / Q000737:chemistry*; D007525:Isoelectric Focusing / Q000379:methods; D014780:Viruses / Q000737:chemistry*