Collection and X-ray microanalysis of airway surface liquid in the mouse using ion exchange beads.
The airway surface liquid (ASL) is a thin layer of liquid covering the airway epithelium. The ionic composition of the ASL is assumed to be important for airway function and may be altered in diseases such as cystic fibrosis and exercise-induced asthma. A method for collection of ASL is presented in which the fluid is collected using small dextran ion-exchange beads. The beads are equilibrated with the ASL in a humidity chamber, collected under silicon oil, dried and analyzed by X-ray microanalysis. Analysis of standard beads prepared by exposure to different salt solutions shows that linear calibration lines can be obtained, but that beads absorb different elements to a different extent. The results show that the ASL in mice is hypotonic, and that the mucus component of the ASL has an elemental composition that is different from that of the periciliary fluid.
Department of Medical Cell Biology, University of Uppsala, BMC, rum B1-118b, Box 571, Husargatan 3, Uppsala SE-75123, Sweden.
Harriet
H
Nilsson
Inna
I
Kozlova
Viengphet
V
Vanthanouvong
Godfried M
GM
Roomans
D000818:Animals; D003550:Cystic Fibrosis / Q000378:metabolism; D003911:Dextrans; D004577:Electron Probe Microanalysis / Q000379:methods*; D005260:Female; D007474:Ion Exchange; D051379:Mice; D014132:Trachea / Q000737:chemistry*
D003911:Dextrans