A novel tumor cell line cloned from mutated human embryonic bone marrow mesenchymal stem cells.
A novel tumor cell line, denominated F6, was established from mutated human embryonic bone marrow mesenchymal stem cells (MSCs) which were induced by the GM-CSF and IL-4 in vitro. The characteristics of the F6 cell line, such as surface antigens, cell cycle, growth curve, gene expression, morphology, cytogenetics and tumor model were analyzed. The F6 cells were round and grew suspended in a plastic dish. The cell line has a strong self-renewal capability, was positive for CD13, CD29, CD44, but negative for CD1alpha, CD3, CD10, CD14, CD23, CD33, CD34, CD38, CD41, CD45, CD54 and HLA-DR. The surface antigens were lower than those of human embryonic MSCs. The karyotype of F6 cells was abnormal. The cell cycle included: G0/G1 phase, 52.24%; G2/M phase, 8.00%; S phase, 41.76%. After the cells had been passaged serially for more than 17 months (62 passages), their characteristics were still retained. The F6 cells resulted in tumors in SCID nude mice in vivo (8/8) and caused metastasis (3/8). The pathologic examination revealed that the tumor cells extensively invaded surrounding normal tissues such as dermis, muscular tissue, nerve tissue, adipose tissue and lymphoid tissue. F6 cell line, tumor tissues derived from F6 cells and the MSCs expressed different levels of the nucleostemin gene. These findings suggested that F6 may be a novel tumor cell line. It may provide evidence for the theory that cancer originates from stem cells, and may be useful for the investigation on safety of human MSCs in the clinical application.
Xu
Wenrong
W
School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China. icls@ujs.edu.cn
Qian
Hui
H
Zhu
Wei
W
Chen
Yongchang
Y
Shao
Qixiang
Q
Sun
Xiaochun
X
Hu
Jiabo
J
Han
Chongxu
C
Zhang
Xiran
X
D000818:Animals; D000941:Antigens / Q000737:chemistry; D001853:Bone Marrow / Q000196:embryology; D001854:Bone Marrow Cells / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D016193:G1 Phase; D019204:GTP-Binding Proteins; D006801:Humans; D007621:Karyotyping; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008854:Microscopy, Electron; D009154:Mutation; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D016192:Resting Phase, Cell Cycle; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D017422:Sequence Analysis, DNA; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D020298:U937 Cells
D000941:Antigens; D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D004247:DNA; D019204:GTP-Binding Proteins