Doxorubicin accumulation in individually electrophoresed organelles.
We report the doxorubicin content in individual organelles following their capillary electrophoretic separation and illustrate that chemical accumulation at the subcellular level is highly heterogeneous. In individual mitochondria from cultured human leukemia cells DOX amount is around 50 zmol, 2 orders of magnitude higher than expected from diffusion during drug treatment, and spans 2 orders of magnitude.
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Adrian B
AB
Anderson
Guohua
G
Xiong
Edgar A
EA
Arriaga
D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000032:analysis / Q000493:pharmacokinetics*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin