Simple and viable in vitro perfusion model for training microvascular anastomoses.
In this report, we describe a novel in vitro perfused microvessel model for training microvascular anastomotic exercises. Arteries and veins with a diameter of ca. 1 mm were explanted from chicken wings. These vessels were cannulated at both ends and mounted on a platform. Preserved, expired whole blood obtained from the blood bank was continuously injected through the proximal catheter, using an automatic perfusor. This in vitro perfused microvessel model exactly simulated the viable small-animal vessels. The setting is very simply and reliably repeated; the materials used are very cheap and universally available. There are no ethical questions involved. Vessels explanted from the human placenta or omentum may be used in a similar manner to gain the "feel" of functioning human microvascular tissue. But such materials are rarer and require the approval of ethical committees.
Department of Neurological Surgery, Carl Gustav Carus University Hospital, Technical University of Dresden, Dresden, Germany. Kartik.Krishnan@uniklinikum-dresden.de
Kartik G
KG
Krishnan
Peter
P
Dramm
Gabriele
G
Schackert
D000714:Anastomosis, Surgical / Q000193:education; D000818:Animals; D002645:Chickens; D066298:In Vitro Techniques; D008866:Microsurgery / Q000193:education*; D010477:Perfusion / Q000379:methods; D013663:Teaching / Q000379:methods*; D014656:Vascular Surgical Procedures / Q000193:education*; D014921:Wings, Animal / Q000098:blood supply*