Analysis of differential gene expression in healthy and osteoarthritic cartilage and isolated chondrocytes by microarray analysis.
The regulation of chondrocytes in osteoarthritic cartilage and the expression of specific gene products by these cells during early-onset and late-stage osteoarthritis are not well characterized. With the introduction of cDNA array technology, the measurement of thousands of different genes in one small tissue sample can be carried out. Interpretation of gene expression analyses in articular cartilage is aided by the fact that this tissue contains only one cell type in both normal and diseased conditions. However, care has to be taken not to over- and misinterpret results, and some major challenges must be overcome in order to utilize the potential of this technology properly in the field of osteoarthritis.
Aigner
Thomas
T
Cartilege Research, Department of Pathology, University of Erlangen-Nürnberg, Erlangen, Germany.
Saas
Joachim
J
Zien
Alexander
A
Zimmer
Ralf
R
Gebhard
Pia M
PM
Knorr
Thomas
T
D002356:Cartilage / Q000737:chemistry / Q000378:metabolism*; D019902:Chondrocytes / Q000737:chemistry / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification
D012313:RNA