High-resolution crystals and preliminary X-ray diffraction studies of a catalytic RNA.
High-resolution single crystals of a catalytic RNA molecule derived from the sequence of the satellite RNA of tobacco ringspot virus have been obtained. The unit-cell volumes of the RNA crystals vary depending on the crystallization conditions and temperature. The best crystal form, when flash frozen, has space group P1 with unit-cell dimensions a = 53.08, b = 71.81, c = 28.03 A, alpha = 98.43, beta = 104.32 and gamma = 74.54 degrees. This form diffracts to a resolution of 2.4 A. A heavy-atom derivative search is in progress.
Kim
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Department of Chemistry and Structural Biology Division of Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Holbrook
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Jancarik
J
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Pandit
J
J
Weng
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X
Bohm
A
A
Kim
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SH