Poly(decyl methacrylate)-based fluorescent PEBBLE swarm nanosensors for measuring dissolved oxygen in biosamples.
150-250 nm Poly(decyl methacrylate)(PDMA) fluorescent ratiometric nanosensors for dissolved oxygen have been developed. Platinum octaethylporphine ketone (PtOEPK), the oxygen-sensitive dye, and octaethylporphyrin (OEP), the oxygen-insensitive dye, have been incorporated into PDMA nanoparticles to make the sensors ratiometric. Based on the corresponding Stern-Volmer plot, these nanosensors exhibit almost complete linearity over the whole range of dissolved molecular oxygen from 0 to 42.5 ppm (deoxygenated to pure oxygen-bubbled water). The overall quenching response is up to 97.5%, the best so far for all dissolved oxygen optical sensors. These PEBBLE nanosensors also show very good reversibility and stability to leaching and photobleaching, as well as very short response times and no perturbation by proteins. In human plasma they demonstrate a robust oxygen sensing capability, little affected by light scattering and autofluorescence. Potential applications include intracellular oxygen imaging and microresolved pressure profiles in biological and other heterogenous environments.
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
Youfu
Y
Cao
Yong-Eun
YE
Lee Koo
Raoul
R
Kopelman
D005456:Fluorescent Dyes; D006801:Humans; D008689:Methacrylates; D036103:Nanotechnology; D043942:Nanotubes; D009944:Organoplatinum Compounds / Q000097:blood*; D010100:Oxygen / Q000032:analysis* / Q000097:blood; D011166:Porphyrins / Q000097:blood*
D005456:Fluorescent Dyes; D008689:Methacrylates; D009944:Organoplatinum Compounds; D011166:Porphyrins; C582846:platinum(II) octaethylporphine ketone; D010100:Oxygen