Transport of amino acid esters and the amino-acid-based prodrug valganciclovir by the amino acid transporter ATB(0,+).
OBJECTIVE
The purpose of this study was to analyze the transport of amino acid esters and the amino-acid-based prodrug valganciclovir by the Na(+)/Cl(-)-coupled amino acid transporter ATB(0,+).


METHODS
The interaction of amino acid esters and valganciclovir with the cloned rat ATB(0,+) was evaluated in a mammalian cell expression system and in the Xenopus oocyte expression system.


RESULTS
In mammalian cells, expression of ATB(0,+) induced glycine uptake. This uptake was inhibited by valine and its methyl, butyl, and benzyl esters. The benzyl esters of other neutral amino acids were also effective inhibitors. Valganciclovir, the valyl ester of ganciclovir, was also found to inhibit ATB(0,+)-mediated glycine uptake competitively. Exposure of ATB(0,+)-expressing oocytes to glycine induced inward currents. Exposure to different valyl esters (methyl, butyl, and benzyl), benzyl esters of various neutral amino acids, and valganciclovir also induced inward currents in these oocytes. The current induced by valganciclovir was saturable with a K0.5 value of 3.1+/-0.7 mM and was obligatorily dependent on Na+ and Cl-. The Na+:Cl-:valganciclovir stoichiometry was 2 or 3:1:1.


CONCLUSIONS
Amino acid esters and the amino-acid-based prodrug valganciclovir are transported by ATB(0,+). This shows that ATB(0,+) can serve as an effective delivery system for amino acid-based prodrugs.
Departments of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia 30912, USA.
Nagavedi S
NS
Umapathy
Vadivel
V
Ganapathy
Malliga E
ME
Ganapathy
D026962:Amino Acid Transport System ASC / Q000378:metabolism*; D021542:Amino Acids, Neutral / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D004952:Esters / Q000493:pharmacokinetics; D015774:Ganciclovir / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D006801:Humans; D015778:Minor Histocompatibility Antigens; D009865:Oocytes / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism; D011355:Prodrugs / Q000493:pharmacokinetics*; D000077562:Valganciclovir; D014982:Xenopus laevis
D026962:Amino Acid Transport System ASC; D021542:Amino Acids, Neutral; D004952:Esters; D015778:Minor Histocompatibility Antigens; D011355:Prodrugs; C420506:SLC1A5 protein, human; D000077562:Valganciclovir; D015774:Ganciclovir