[Isolation and partial characterization of DNA topoisomerase I from the nucleoids of white mustard chloroplasts].
DNA topoisomerase was isolated for the first time from nucleoids of white mustard (Sinapis alba L.) chloroplasts. The enzyme had a molecular weight of 70 kDa; it was ATP-independent, required the presence of mono- (K+) and bivalent (Mg2+) cations, and was capable of relaxing both negatively and positively supercoiled DNA. These results suggest that the enzyme isolated belongs to type IB DNA topoisomerases.
Belkina
G G
GG
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia.
Pogul'skaia
E V
EV
Iurina
N P
NP
D002412:Cations; D002467:Cell Nucleus / Q000201:enzymology; D002498:Centrifugation; D002736:Chloroplasts / Q000201:enzymology*; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism; D008274:Magnesium; D008970:Molecular Weight; D010957:Plasmids; D011188:Potassium; D031226:Sinapis / Q000201:enzymology*
D002412:Cations; D004278:DNA, Superhelical; D004264:DNA Topoisomerases, Type I; D008274:Magnesium; D011188:Potassium