Alanine-threonine polymorphism of Helicobacter pylori RpoB is correlated with differential induction of interleukin-8 in MKN45 cells.
Geographical differences in the genetic diversity of Helicobacter pylori isolates were examined by analyzing rpoB sequences. An extremely high level of allelic diversity among H. pylori strains was found. The rpoB sequences of Asian and non-Asian (North and South American, European, and South African) strains were found to differ. An amino acid polymorphism (alanine and threonine RpoB types) was found at the 497th residue by deduced amino acid analysis. RpoB with a threonine residue (RpoB(Thr)) was uniquely present in East Asian countries, and two-thirds of the H. pylori isolate population in this region was RpoB(Thr); however, this type was rare or absent in Western countries, where RpoB(Ala) predominated. RpoB(Thr) strains induced a much larger amount of interleukin-8, a chemokine that plays an important role in chronic inflammation, than RpoB(Ala) strains in cultured MKN45 cells.
Lee
Keun-Hwa
KH
Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-799, Korea.
Cho
Myung-Je
MJ
Yamaoka
Yoshio
Y
Graham
David Y
DY
Yun
Yeo-Jun
YJ
Woo
So-Yon
SY
Lim
Chang-Young
CY
Ko
Kwan-Soo
KS
Kim
Bum-Joon
BJ
Jung
Hyun-Chae
HC
Lee
Woo-Kon
WK
Rhee
Kwang-Ho
KH
Kook
Yoon-Hoh
YH
D000409:Alanine; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018920:Coculture Techniques; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000276:immunology; D005843:Geography; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016209:Interleukin-8 / Q000096:biosynthesis*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013912:Threonine
D017931:DNA Primers; D004269:DNA, Bacterial; D016209:Interleukin-8; D013912:Threonine; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D000409:Alanine