A role for DNA polymerase delta in gene conversion and crossing over during meiosis in Saccharomyces cerevisiae.
A screen for mutants of budding yeast defective in meiotic gene conversion identified a novel allele of the POL3 gene. POL3 encodes the catalytic subunit of DNA polymerase delta, an essential DNA polymerase involved in genomic DNA replication. The new allele, pol3-ct, specifies a protein missing the last four amino acids. pol3-ct shows little or no defect in DNA replication, but displays a reduction in the length of meiotic gene conversion tracts and a decrease in crossing over. We propose a model in which DNA synthesis determines the length of strand exchange intermediates and influences their resolution toward crossing over.
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06520-8103, USA.
Laurent
L
Maloisel
Jaya
J
Bhargava
G Shirleen
GS
Roeder
D000483:Alleles; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic / Q000235:genetics*; D017931:DNA Primers; D005656:Fungal Proteins / Q000235:genetics*; D005720:Gamma Rays; D005785:Gene Conversion / Q000235:genetics*; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D013045:Species Specificity; D014466:Ultraviolet Rays
D017931:DNA Primers; D005656:Fungal Proteins