Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary.
Previously, bone morphogenetic protein-7 (BMP-7) was suggested as a factor that may act to facilitate the transition of follicles from primordial stage to the pool of developed primary, preantral, and antral follicles (Lee et al. 2001: Biol Reprod 65:994-999.). Thus, aim of the present study was to evaluate effect(s) of BMP-7 on the primordial-primary follicle transition. Neonatal mouse ovaries were cultured in the presence or absence of 100 mIU/ml FSH with various doses of BMP-7 (0, 10, and 100 ng/ml). After 4-day culture period, number of follicles was counted and the expression of transcripts for FSH receptor (FSHR), kit ligand (KL), and c-kit was measured by RT-PCR. BMP-7 alone at 100 ng/ml concentration stimulated follicle development with concurrent increase of mRNA for FSHR. BMP-7 alone down-regulated KL expression however, the ratio between KL1 and KL2 was increased. There was no change in the c-kit mRNA expression. Results of the present study suggest that the BMP-7 is one of the factors involved in primordial-primary follicle transition in the mouse ovary and it may play a role in expression of FSHR for further follicular development.
Lee
Woo-Sik
WS
Infertility Medical Center, CHA General Hospital, Seoul, Korea.
Yoon
Se-Jin
SJ
Yoon
Tae-Ki
TK
Cha
Kwang-Yul
KY
Lee
Sook-Hwan
SH
Shimasaki
Shunichi
S
Lee
Sangho
S
Lee
Kyung-Ah
KA
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000378:metabolism / Q000502:physiology*; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D011962:Receptors, FSH / Q000096:biosynthesis; D019089:Stem Cell Factor / Q000096:biosynthesis; D016212:Transforming Growth Factor beta / Q000502:physiology*
D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011962:Receptors, FSH; D019089:Stem Cell Factor; D016212:Transforming Growth Factor beta; D005640:Follicle Stimulating Hormone; D019009:Proto-Oncogene Proteins c-kit