[Level of tissue plasminogen activator protein in pulmonary artery in acute pulmonary thromboembolism in rabbit].
OBJECTIVE
To study the changes in tissue plasminogen activator(t-PA) protein in pulmonary artery and its clinical significance after acute pulmonary thromboembolism (PTE).


METHODS
Thirty rabbits were randomly divided into four groups after replicating a model of acute PTE in rabbit by thrombi occlusion method. Specimens were obtained from both normal and morbid pulmonary artery 3, 8 and 24 hours after APE, and protein contents of t-PA were determined using immunohistochemical method.


RESULTS
A few endothelial cells and smooth muscle cells of the normal pulmonary artery were positive for t-PA. After 3 hours of PTE, there was no significant changes in t-PA positive stain among embolismic, non-embolismic and normal pulmonary artery. After 8 and 24 hours of PTE, strong positive staining was found in the residual endothelial cells and a part of smooth muscle cells (all P<0.01).


CONCLUSIONS
There is significantly strong positive staining for t-PA in the pulmonary artery wall after pulmonary embolism, implying that the local fibrinolysis activity was enhanced, and it might be helpful for lysis of the embolus.
Zhang
Jing-xia
JX
TianJin Thoracic Hospital, TianJin 300051, China.
Sun
Gen-yi
GY
Chen
Yong-li
YL
Wang
Pei-xian
PX
Yuan
Zhi-ming
ZM
Zhou
Yu-ling
YL
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism*; D011655:Pulmonary Embolism / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D010959:Tissue Plasminogen Activator