Screening of Y chromosome microdeletion which contains AZF regions in 71 Turkish azoospermic men.
Screening of Y chromosome microdeletion which contains AZF regions in 71 turkish azoospermic men: In 71 Turkish men Y chromosome microdeletions have been studied before intracytoplasmic sperm injection (ICSI). DNA samples were amplified with 18 STS primers of the azoospermia factor (AZF) region on the Y chromosome by using multiplex polymerase chain reaction (PCR). Microdeletions were detected in 4 azoospermic men (5.6 %); one with a deletion in the AZFb region, while the 3 others had a large deletion extending over multiple chromosomal regions (AZFb+c+d and AZFa+b+c+d). In the patients with microdeletion, no spermatogenetic activity could be detected in testis biopsies. This result confirms the idea that Y chromosome microdeletion analysis is important in investigating the possibility of finding sperm in testicular sperm extraction (TESE). Therefore, we point out the importance of genetic testing and counselling regarding Y chromosome microdeletion for couples requesting ICSI.
Ege University, Faculty of Science, Department of Biology, Izmir, Turkey.
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Okutman-Emonts
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Pehlivan
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Tavmergen
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Tavmergen-Goker
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Ozkinay
D016022:Case-Control Studies; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D056426:Genetic Loci; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000453:epidemiology / Q000235:genetics*; D029589:Seminal Plasma Proteins / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology
D029589:Seminal Plasma Proteins