RNA interference directed against Poly(ADP-Ribose) polymerase 1 efficiently suppresses human immunodeficiency virus type 1 replication in human cells.
We established small interfering RNA (siRNA) directed against poly(ADP-ribose) polymerase 1 (PARP-1) that effectively reduces the expression of PARP-1 in two human cell lines. Established siRNA against PARP-1 significantly suppressed human immunodeficiency virus type 1 (HIV-1) replication, as well as the activation of the integrated HIV-1 long terminal repeat promoter. These results indicate that PARP-1 is required for efficient HIV-1 replication in human cells. We propose that PARP-1 may serve as a cellular target for RNA interference-mediated gene silencing to inhibit HIV-1 replication.
Department of Biochemistry, Nara Medical University, Shijo 840, Kashihara, Nara 364-8521, Japan. mkameoka@naramed-u.ac.jp
Masanori
M
Kameoka
Souichi
S
Nukuzuma
Asako
A
Itaya
Yasuharu
Y
Tanaka
Katsuya
K
Ota
Kazuyoshi
K
Ikuta
Koichiro
K
Yoshihara
D002460:Cell Line; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D014779:Virus Replication
D034741:RNA, Small Interfering; D011065:Poly(ADP-ribose) Polymerases