Magnetite nanoparticle-loaded anti-HER2 immunoliposomes for combination of antibody therapy with hyperthermia.
Anti-HER2 antibody can induce antitumor responses, and can be used in delivering drugs to HER2-overexpressing cancer. Previously, we produced hyperthermia using magnetite nanoparticles that generate heat in an alternating magnetic field. In the present study, we constructed anti-HER2 immunoliposomes containing magnetite nanoparticles, which act as tumor-targeting vehicles, combining anti-HER2 antibody therapy with hyperthermia. The magnetite nanoparticle-loaded anti-HER2 immunoliposomes exerted HER2-mediated antiproliferative effects on SKBr3 breast cancer cells in vitro. Moreover, 60% of magnetite nanoparticles were incorporated into SKBr3, and the cells were then heated at 42.5 degrees C under an alternating magnetic field, resulting in strong cytotoxic effects. These results suggest that this novel therapeutic tool is applicable to treatment of HER2-overexpressing cancer.
Department of Biotechnology, School of Engineering, Nagoya University, Chikusa-ku, Furo-cho, 464-8603, Japan.
Akira
A
Ito
Yuko
Y
Kuga
Hiroyuki
H
Honda
Hiroyuki
H
Kikkawa
Atsushi
A
Horiuchi
Yuji
Y
Watanabe
Takeshi
T
Kobayashi
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism / Q000628:therapy; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D003131:Combined Modality Therapy; D004305:Dose-Response Relationship, Drug; D052203:Ferrosoferric Oxide; D005334:Fever; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007501:Iron; D008081:Liposomes / Q000737:chemistry*; D008280:Magnetics; D010087:Oxides; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D000068878:Trastuzumab
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D052203:Ferrosoferric Oxide; D007501:Iron; D008081:Liposomes; D010087:Oxides; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab