Overexpression of delta-like (Dlk) in a subset of myelodysplastic syndrome bone marrow trephines.
The mRNA expression level of the gene delta-like (Dlk), coding for a signal transducer related to the Delta-Notch family, was measured using real-time PCR methodology in a large series of control biopsies (n = 61) and bone marrow trephines from patients with myelodysplastic syndrome (MDS) (n = 93) and related myeloid disorders (AML, n = 16 and chronic myeloproliferative disease (CMPD), n = 38). It turned out that dlk is strongly overexpressed in a subset of MDS (16%) cases, however with higher frequency in blast rich MDS cases. Therefore, the quantitative detection of dlk mRNA can support the morphological diagnosis of MDS in selected cases.
Länger
Florian
F
Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, Hannover, Germany.
Stickel
Juliane
J
Tessema
Mathewos
M
Kreipe
Hans
H
Lehmann
Ulrich
U
D001856:Bone Marrow Examination / Q000379:methods; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C064045:delta protein