Anaerobic 2-propanol degradation in anoxic paddy soil and the possible role of methanogens in its degradation.
The anaerobic degradation of 2-propanol in anoxic paddy soil was studied with soil cultures and a 2-propanol-utilizing methanogen. Acetone was the first and the major intermediate involved in the methanogenic degradation of 2-propanol. Analyses with a methanogenesis inhibitor, bacteria antibiotics, and the addition of H2 to the gas phase revealed that 2-propanol oxidation to acetone directly occurred using 2-propanol-utilizing methanogens, but not with H2-producing syntrophic bacteria, for which the removal of acetone is required for complete 2-propanol oxidation. The 2-propanol-utilizing strain IIE1, which is phylogenetically closely related to Methanoculleus palmolei, was isolated from paddy soil, and the potential role of the strain in 2-propanol degradation was investigated. 2-Propanol is one of the representative fermentation intermediates in anaerobic environments. This is the first report on the anaerobic 2-propanol degradation process.
Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8224, Japan. symbio@cc.hirosaki-u.ac.jp
Akio
A
Tonouchi
D019840:2-Propanol / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D000096:Acetone / Q000378:metabolism; D000667:Ampicillin / Q000494:pharmacology; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002725:Chloroform / Q000494:pharmacology; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D019638:Methanomicrobiaceae / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014640:Vancomycin / Q000494:pharmacology
D000085:Acetates; D000900:Anti-Bacterial Agents; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000096:Acetone; D014640:Vancomycin; D000667:Ampicillin; D002725:Chloroform; D006859:Hydrogen; D019840:2-Propanol; D008697:Methane