[Research of the amounts of flavonoids accumulated in the buds of single-styled hawthorn].
OBJECTIVE
To determine the amounts of flavonoids accumulated in the buds of single-styled hawthorn (Crataegus monogyna Jacq.); and to establish the possibility of usage of hawthorn buds in pharmacy practice.


METHODS
Different examples of hawthorn buds collected in 2001, 2002 and 2003 from some Lithuanian regions: Akmene (V1, V2, V3), Kedainiai (S1, S2, S3), Klaipeda (M1), Lazdijai (D1, D2, D3) and Vilnius (VL1, VL2) from branches of lower storey. Examples were extracted with ethanol and flavonoids were measured by spectroscopic method and by high performance liquid chromatography.


RESULTS
From 0.72 to 1.89% of flavonoids are found in the hawthorn buds. The dominating flavonoid is vitexin-2-O-rhamnosid (6.72-10.91 milligrams in one gram of dried crude drug). Other flavonoids are: vitexin: 0.88-6.53 milligrams/gram, hyperosid: 0.85-2.70 milligrams/gram, rutin: 0.72-2.10 milligrams/gram and quercitrin: 0.82-1.01 milligrams/gram.


CONCLUSIONS
The marks of phytochemical compounds of Lithuanian single-styled hawthorn buds corroborated the theory of possibility to use hawthorn buds as pharmaceutical crude drug and form sufficient ground for planning phytochemical and pharmacological researches of new hawthorn crude drug.
Jakstas
Valdas
V
Department of Pharmaceutical Chemistry and Pharmacognosy, Kaunas University of Medicine, A. Mickeviciaus 9, 44307 Kaunas, Lithuania. valdas.jakstas@med.kmu.lt
Janulis
Valdimaras
V
Labokas
Juozas
J
D047310:Apigenin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D006801:Humans; D008097:Lithuania; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000032:analysis; D012106:Research; D012431:Rutin / Q000032:analysis
D005419:Flavonoids; C021329:vitexin rhamnoside; D012431:Rutin; D047310:Apigenin; D011794:Quercetin; C032731:vitexin