Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis.
Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland.
Ossi
O
Turunen
Janne
J
Jänis
Fred
F
Fenel
Matti
M
Leisola
D019943:Amino Acid Substitution; D001407:Bacillus / Q000201:enzymology; D001665:Binding Sites; D004220:Disulfides / Q000032:analysis; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods; D017433:Protein Structure, Secondary; D013816:Thermodynamics; D014242:Trichoderma / Q000201:enzymology; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D004220:Disulfides; D014995:Xylosidases