Overexpression of delta-like (Dlk) in a subset of myelodysplastic syndrome bone marrow trephines.
The mRNA expression level of the gene delta-like (Dlk), coding for a signal transducer related to the Delta-Notch family, was measured using real-time PCR methodology in a large series of control biopsies (n = 61) and bone marrow trephines from patients with myelodysplastic syndrome (MDS) (n = 93) and related myeloid disorders (AML, n = 16 and chronic myeloproliferative disease (CMPD), n = 38). It turned out that dlk is strongly overexpressed in a subset of MDS (16%) cases, however with higher frequency in blast rich MDS cases. Therefore, the quantitative detection of dlk mRNA can support the morphological diagnosis of MDS in selected cases.
Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, Hannover, Germany.
Florian
F
Länger
Juliane
J
Stickel
Mathewos
M
Tessema
Hans
H
Kreipe
Ulrich
U
Lehmann
D001856:Bone Marrow Examination / Q000379:methods; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C064045:delta protein