c-Fos as a regulator of degranulation and cytokine production in FcepsilonRI-activated mast cells.
The AP-1 complex is composed of c-Jun and c-Fos and is a key component in the regulation of proinflammatory genes. Mast cells play a significant role in the initiation of many inflammatory responses, such as allergy and allergy-associated diseases. In the present work, we characterized the role of c-Fos in mast cell function by investigating IL-3-dependent cell proliferation, degranulation capability, and cytokine expression in c-Fos-deficient mice. In c-Fos-deficient mast cells, we found that FcepsilonRI-mediated degranulation was significantly inhibited, which correlates with the reduced expression of SWAP-70, VAMP-7, and Synaptotagmin I genes, which are involved directly in the degranulation process. These findings show that c-Fos plays an important role in FcepsilonRI-mediated regulation of mast cell function.
Department of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Yu-Nee
YN
Lee
Jan
J
Tuckerman
Hovav
H
Nechushtan
Gunter
G
Schutz
Ehud
E
Razin
Peter
P
Angel
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D015550:Cell Degranulation / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005434:Flow Cytometry; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis; D007377:Interleukin-3 / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D015778:Minor Histocompatibility Antigens; D018448:Models, Immunological; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000096:biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D017455:Receptors, IgE / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology; D050863:Synaptotagmin I; D050857:Synaptotagmins
D002135:Calcium-Binding Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015778:Minor Histocompatibility Antigens; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D017455:Receptors, IgE; C113365:Swap70 protein, mouse; D050863:Synaptotagmin I; C494311:Syt1 protein, mouse; D050857:Synaptotagmins