Overexpression of chromosomal genes in Escherichia coli.
Conversion of some carbon sources into desired compounds by a biological system is the goal of many biotechnologists. The understanding of the mechanisms by which an organism does these conversions permits the improved production of specific metabolites. Pathway engineering involves the strategies to modify cells to overproduce desired molecules. We describe here the methodology to modify chromosomal genes by replacing their native regulatory regions with promoter cassettes to increase or deregulate expression of chromosomal genes.
Valle
Fernando
F
Department of Metabolic Pathway Engineering, Genencor International Inc., Palo Alto, CA, USA.
Flores
Noemí
N
D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005809:Genes, Regulator; D017930:Genes, Reporter; D010957:Plasmids; D011401:Promoter Regions, Genetic