Interaction of ACE2 and integrin beta1 in failing human heart.
ACE2 purified from failing human heart was found to form a complex with integrin beta1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 microM and a Vmax of 2.13 pmol/min/microl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.
The Center for Functional Genomics, Proteomics Core Facility, University at Albany, Rensselaer, NY, USA.
Qishan
Q
Lin
Rebecca S
RS
Keller
Barbara
B
Weaver
Lawrence S
LS
Zisman
D000595:Amino Acid Sequence; D000085962:Angiotensin-Converting Enzyme 2; D002268:Carboxypeptidases / Q000378:metabolism*; D009202:Cardiomyopathies / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A
D019012:Integrin beta1; D002268:Carboxypeptidases; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2