Crystallization of a Humicola lanuginosa lipase-inhibitor complex with the use of polyethylene glycol monomethyl ether.
The fungal Humicola lanuginosa lipase complexed with the inhibitor n-dodecylphosphonate ethyl ester was crystallized in space group P2(1)2(1)2(1) with pseudotetragonal unit-cell parameters of a = 131.7 (2), b = 131.3 (1), c = 75.4 (1) A. 92% of X-ray diffraction data to 2.8 A resolution were collected with a final R(merge) = 8.5%. The crystals were grown using a new kind of precipitant - polyethylene glycol monomethyl ether (Peg-mme) of molecular weight 5000.
Department of Chemistry, University of York, Heslington, England.
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Brzozowski