Type II collagen peptides for measuring cartilage degradation.
This paper describes two new immunoassays for a peptide of the triple helix of type II collagen (Coll 2-1) and its nitrated form (Coll 2-1 NO(2)). In healthy subjects aged between 20 and 65 years old, Coll 2-1 and Coll 2-1 NO(2) levels in serum were in means 125.13+/-3.71 and 0.16+/-0.08 nmol/l, respectively. These levels did not significantly vary with age. However, up to 45 years of age, Coll 2-1 NO(2) levels in women were significantly higher than in men. In patients with knee osteoarthritis (OA), Coll 2-1 in serum was found to be elevated compared to healthy controls (267.45+/-26.42 nmol/l vs 126.78+/-6.61 nmol/l). Further, we have demonstrated that an increase of the urinary levels of Coll 2-1 or Coll 2-1 NO(2) over 1 year was predictive of joint space narrowing progression in OA patients. In conclusion, these preliminary results indicate that Coll 2-1 could be a predictive marker of knee OA progression.
Bone and Cartilage Research Unit, Institute of Pathology, CHU Sart-Tilman, 4000 Liège, Belgium. yhenrotin@ulg.ac.be
Y
Y
Henrotin
M
M
Deberg
J-E
JE
Dubuc
E
E
Quettier
S
S
Christgau
J-Y
JY
Reginster
D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002358:Cartilage, Articular / Q000473:pathology*; D016022:Case-Control Studies; D024043:Collagen Type II / Q000097:blood* / Q000652:urine; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood / Q000473:pathology*; D017698:Postmenopause / Q000097:blood / Q000652:urine; D011817:Rabbits; D012737:Sex Factors
D015415:Biomarkers; C499177:COL2A1 protein, human; D024043:Collagen Type II