EC_oligos: automated and whole-genome primer design for exons within one or between two genomes.
CONCLUSIONS
EC_oligos designs oligonucleotides (oligos) from exons of annotated genomic sequence information. It can automatically and rapidly select oligos that are conserved between two sets of sequence data, and can pair up oligos for use as PCR primers. It can do this on a whole-genome scale and according to user-defined criteria.


BACKGROUND
The source code, executable program and user manual are available at ftp://ftp.ebi.ac.uk/pub/software/dos/EC_oligos/.
Department of Plant Science, McGill University, Ste-Anne-de-Bellevue, Montreal, QC, Canada.
Shaolin
S
Liu
Nicholas A
NA
Tinker
Stephen J
SJ
Molnar
Diane E
DE
Mather
D000465:Algorithms; D002874:Chromosome Mapping / Q000379:methods*; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics*; D005091:Exons / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D017931:DNA Primers