Sub-cellular proteomic analysis of a Medicago truncatula root microsomal fraction.
Since the last decade, Medicago truncatula has emerged as one of the model plants particularly investigated in the field of plant-microbe interactions. Several genetic and molecular approaches including proteomics have been developed to increase knowledge about this plant species. To complement the proteomic data, which have mainly focused on the total root proteins from M. truncatula, we carried out a sub-cellular approach to gain access to the total membrane-associated proteins. Following the setting up of the purification process, microsomal proteins were separated on 2-DE. Ninety-six out of the 440 well-resolved proteins were identified by MALDI-TOF peptide mass fingerprinting. A high percent (83%) of successful protein identification was obtained when using M. truncatula clustered EST database for queries. During the purification process, the enrichment in membrane-associated proteins was monitored on 2-D gels. The membrane location of microsomal proteins was further confirmed using PMF identification. This study reports a fractionation process for characterizing microsomal root proteins of M. truncatula, which could be an interesting tool for investigating the molecular mechanisms involved in root symbioses.
Valot
Benoît
B
UMR 1088 INRA/CNRS 5184/UB Plante-Microbe-Environment, INRA/CMSE, BP 86510, 21065 Dijon, France.
Gianinazzi
Silvio
S
Eliane
Dumas-Gaudot
DG
D015180:Electrophoresis, Gel, Two-Dimensional; D029902:Medicago / Q000378:metabolism*; D010449:Peptide Mapping; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D018517:Plant Roots / Q000378:metabolism*; D040901:Proteomics; D013559:Symbiosis
D010940:Plant Proteins