Copper deficiency induced expression of Fe-superoxide dismutase gene in Matteuccia struthiopteris.
Iron-superoxide dismutase (Fe-SOD) activity was not detected in extracts from the leaves of ferns, Equisetum arvense and Matteuccia struthiopteris. To know why ferns lack Fe-SOD activity, the Fe-SOD like gene (MsFeSOD1) was isolated from M. struthiopteris and its expression was investigated with a focus on the metals Fe and Cu using the prothalli of the fern. The expression of MsFeSOD1 mRNA was induced by a deficiency of Cu, but Fe-SOD activity was not detected. The recombinant protein of MsFeSOD1 produced in E. coli showed Fe-SOD activity. These findings suggest that the fern Fe-SOD like gene was transcriptionally regulated by Cu but an additional mechanism is involved in the formation of an active enzyme.
Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan.
Kenichi
K
Murao
Masayuki
M
Takamiya
Kanji
K
Ono
Hiroyoshi
H
Takano
Susumu
S
Takio
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D003300:Copper / Q000172:deficiency / Q000494:pharmacology / Q000502:physiology*; D029624:Ferns / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics*
D011994:Recombinant Proteins; D003300:Copper; D013482:Superoxide Dismutase