Crystallization and preliminary X-ray studies of azurin-I and azurin-II from denitrifying bacterium Alcaligenes xylosoxidans GIFU 1051.
Two distinct azurins named azurin-I and azurin-II were isolated from Alcaligenes xylosoxidans GIFU 1051 (AzG-I and AzG-II). They have been purified and crystallized. The crystals of AzG-I belong to the monoclinic crystal system, space group C2, unit-cell parameters a = 130.6, b = 54.4, c = 74.7 A, beta = 96.1 degrees. The crystals of AzG-II belong to the tetragonal crystal system, space group P4(1)22 or P4(3)22, unit-cell parameters a = b = 52.6, c = 100.7 A. Both crystals diffract up to 2.0 A resolution and are suitable for X-ray crystallographic studies.
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Suita, Japan.
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Li
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Inoue
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Gotowda
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Suzuki
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Yamaguchi
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Kataoka
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Kai