A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor.
A Nepalese heterozygous carrier of a CCR5 mutant, designated 118delF, was characterized. There was a 3 basepair deletion at 352-354 in the CCR5 open reading frame, resulting in the deletion of the phe-118 residue located in the third transmembrane domain. The mutant protein has retained antigen specificity near the third extra-cellular loop (ECL3), but that of ECL2 is markedly reduced. The mutation has also abrogated HIV co-receptor activity. Clinically, the HIV disease had progressed slowly.
HIV Research Laboratories, Department of Microbiology, the University of Hong Kong, Hong Kong, China.
Xiu-Ying
XY
Zhao
Shui-Shan
SS
Lee
Ka-Hing
KH
Wong
Kenny Chi-Wai
KC
Chan
Zhi-Min
ZM
He
Selene
S
Ma
Fai
F
Ng
Chris Chung-Sing
CC
Chan
Tina
T
Ho
Mun-Hon
MH
Ng
Bo-Jian
BJ
Zheng
D000595:Amino Acid Sequence; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006579:Heterozygote; D006801:Humans; D009154:Mutation / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology; D015728:Receptors, HIV / Q000235:genetics* / Q000276:immunology; D017384:Sequence Deletion
D012333:RNA, Messenger; D012367:RNA, Viral; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D015728:Receptors, HIV