Proteomics to display tissue repair opposing injury response to LPS-induced liver injury.
OBJECTIVE
To examine the protein expression alterations in liver injury/repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver injury.


METHODS
Male BALB/c mice were treated with intra-peritoneal (i.p.) LPS (4 mg/kg) and sacrificed at 0, 6, 24 and 30 h to obtain livers. The livers were stained with hematoxylin and eosin for histopathologic analyses. Total liver protein was separated by two-dimensional gel electrophoresis (2-DE). The peptide mass of liver injury or repair related proteins were drawn up and the protein database was searched to identify the proteins.


RESULTS
Observations were as follows: (1) TRAIL-R2 was down regulated in livers of LPS-treated mice. TNFAIP1 was significantly up regulated at 6 h, then down- regulated at 24, 30 h with silent expression during senescent stage. (2) The amount of metaxin 2 and mitochondria import inner membrane translocase subunit TIM8a (TIMM8A) was increased upon treatment with LPS. (3) P34 cdc2 kinase was significantly up-regulated 30 h after LPS administration with silent expression during senescent, 6, 24 h treated stage. (4) The amount of proteasome activator 28 alpha subunit (PA28), magnesium dependent protein phosphatase (MDPP) and lysophospholipase 2 was decreased 6 h after LPS treatment but recovered or up-regulated 24 and 30 h after LPS treatment.


CONCLUSIONS
LPS-treated mouse liver displaying a time-dependent liver injury can result in expression change of some liver injury or repair related proteins.
Department of Gastroenterology, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
Xiao-Wei
XW
Liu
Fang-Gen
FG
Lu
Guang-Sen
GS
Zhang
Xiao-Ping
XP
Wu
Yu
Y
You
Chun-Hui
CH
Ouyang
Dong-Ye
DY
Yang
D000818:Animals; D015415:Biomarkers; D015180:Electrophoresis, Gel, Two-Dimensional; D008070:Lipopolysaccharides / Q000633:toxicity*; D008107:Liver Diseases / Q000473:pathology / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics; D015398:Signal Transduction / Q000502:physiology
D015415:Biomarkers; D008070:Lipopolysaccharides