Use of chemical characteristics to predict the relative bioavailability of supplemental organic manganese sources for broilers.
Twelve organic Mn sources and MnSO4 were evaluated by polarographic analysis and via solubility in buffers (pH 5 and 2) and deionized water. Fractions from solubility tests were evaluated by gel filtration chromatography for structural integrity. Organic Mn sources included five Mn methionine complexes (Mn Met A to Mn Met E), two Mn proteinates (Mn Pro A and Mn Pro B), and five Mn amino acids (Mn AA A to Mn AA E). Sources varied considerably in chemical characteristics. Chelation strength (Qf) ranged from weak (1.9 Qf-values) to strong complexes (115.4 Qf-values). No complexed Mn was found in filtrates at pH 2.0 or 5.0. A 42-d bioassay was used to estimate relative bioavailability of Mn sources for chicks fed diets supplemented with 60, 120, or 180 mg Mn/kg. Bone Mn, heart Mn, heart manganese-superoxide dismutase activity (MnSOD), and heart MnSOD mRNA increased (P < 0.001) as dietary Mn increased. Only heart MnSOD mRNA tended (P < 0.10) to differ among dietary Mn sources. For bioassays of Mn, the MnSOD mRNA level in heart was more sensitive than the MnSOD activity in heart or other indices. Relative to MnSO4 (assigned 100%), slope ratios of MnSOD mRNA levels in heart gave bioavailabilities of 99, 132, and 113% for Mn Met E, Mn AA B, and Mn AA C sources with weak, moderate, and strong chelation strength, respectively. The bioavailability of Mn was more closely related to chelation strength as measured by polarography than to chemical traits assessed by solubility or structural integrity.
Li
S
S
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Luo
X
X
Liu
B
B
Crenshaw
T D
TD
Kuang
X
X
Shao
G
G
Yu
S
S
D000821:Animal Feed; D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D001682:Biological Availability; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D002850:Chromatography, Gel / Q000662:veterinary; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005782:Gels / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption / Q000187:drug effects; D008297:Male; D008345:Manganese / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D009206:Myocardium / Q000201:enzymology; D011897:Random Allocation; D012995:Solubility; D013482:Superoxide Dismutase / Q000378:metabolism
D005782:Gels; D008345:Manganese; D013482:Superoxide Dismutase