A Sensitive Autoradlographic Procedure for Factor VIII-Related Antigen in Canine Plasma.
A second-antibody autoradiographic technique was found to be suitable for visualization of precipitin arcs produced by the electroimmunoassay or crossed immunoelectrophoresis of factor VIII-related antigen in canine plasma. With the new autoradiographic procedure the electroimmunoassay was approximately ten times more sensitive than with the commonly used protein stain, Coomassie Blue R 250. Unlike earlier autoradiographic techniques, second-antibody autoradiography requires only commercially available reagents.
Department of Veterinary Pathology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211.
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Johnson