Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae.
Checkpoint controls ensure the completion of cell cycle events with high fidelity in the correct order. Here we show the existence of a novel checkpoint that ensures coupling of cell wall synthesis and mitosis. In response to a defect in cell wall synthesis, S. cerevisiae cells arrest the cell-cycle before spindle pole body separation. This arrest results from the regulation of the M-phase cyclin Clb2p at the transcriptional level through the transcription factor Fkh2p. Components of the dynactin complex are required to achieve the G2 arrest whilst keeping cells highly viable. Thus, the dynactin complex has a function in a checkpoint that monitors cell wall synthesis.
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Masaya
M
Suzuki
Ryoji
R
Igarashi
Mizuho
M
Sekiya
Takahiko
T
Utsugi
Shinichi
S
Morishita
Masashi
M
Yukawa
Yoshikazu
Y
Ohya
D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism*; D019926:Cyclin B / Q000235:genetics; D000072159:Dynactin Complex; D051858:Forkhead Transcription Factors; D016195:G2 Phase; D005786:Gene Expression Regulation; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D008941:Spindle Apparatus; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic
C093749:CLB2 protein, S cerevisiae; D018797:Cell Cycle Proteins; D019926:Cyclin B; D000072159:Dynactin Complex; C464958:Fkh2 protein, S cerevisiae; D051858:Forkhead Transcription Factors; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors