Micropatterns of spores displaying heterologous proteins.
Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.
Park
Tae Jung
TJ
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea.
Lee
Kyung-Bok
KB
Lee
Seok Jae
SJ
Park
Jong Pil
JP
Lee
Zee-Won
ZW
Choi
Soo-Keun
SK
Jung
Heung-Chae
HC
Pan
Jae-Gu
JG
Lee
Sang Yup
SY
Choi
Insung S
IS
D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013171:Spores, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins