Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe.
Sets of serum and milk samples were collected from various countries and prepared, lyophilised and distributed by 1 laboratory to 12 reference laboratories in Europe. The serum sets contained the three European bovine herpesvirus 1 (BHV1) reference serum samples (EU1, EU2 and EU3), serum samples from naturally and experimentally BHV1-infected cattle, from vaccinated, and vaccinated-challenged cattle, from uninfected cattle, and a series of serum dilutions. In addition, sets of milk samples were distributed. The samples were tested for antibodies against BHV1 in virus neutralisation tests, in gB-specific ELISAs, in indirect ELISAs and in gE-specific ELISAs. It was found that the virus neutralisation test and the gB-specific ELISAs were most sensitive for the detection of antibodies in serum, whereas for assaying milk samples the indirect ELISAs were the tests of choice. The results show that the quality of most laboratories appeared to be adequate, but that one laboratory performed considerably below an acceptable level of quality. Four samples from the panel have been proposed that might be selected as reference sera in addition to the three European reference samples.
Central Institute for Animal Disease Control, Wageningen University and Research Centre, P.O. Box 2004, 8203 AA Lelystad, The Netherlands. hans.kramps@wur.nl
Johannes A
JA
Kramps
Malcolm
M
Banks
Martin
M
Beer
Pierre
P
Kerkhofs
Myriam
M
Perrin
Gerard J
GJ
Wellenberg
Jan T Van
JT
Oirschot
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005060:Europe; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis*; D007753:Laboratories / Q000592:standards; D008892:Milk / Q000276:immunology* / Q000821:virology; D009500:Neutralization Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D011786:Quality Control; D012016:Reference Values; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins