[The application of the gene-recombinated cell in the detection of PCBs compounds in environmental samples].
To develop novel and rapid bioassay systems for detection of PCBs in environmental and biological samples, two lines of gene-recombinated cells containing reporter gene, green fluorescent protein (GFP) and luciferase (Luc), were be chosen to detect the PCBs in the water, sediment and biological samples from environment. The result showed that the correlation between RFU of GFP and Luc activity and PCBs standard sample concentration was fine, r was 0.99188 and 0.98239 respectively. Compared with the instrument analysis with GC-ECD, the correlation between RFU of GFP and Luc activity and PCBs concentration in environmental and biological extracts was also fine. The method offered a new way of screening and semi-quantitative bioassay for PCBs compounds in environmental and biological samples.
Xu
Ting
T
School of the Environment, Nanjing University, Nanjing 210093, China.
Kong
Fan-xiang
FX
Sun
Cheng
C
Tan
Jian-kang
JK
Han
Xiao-bo
XB
Yan
Rong
R
D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D008156:Luciferases; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D011994:Recombinant Proteins / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004785:Environmental Pollutants; D011994:Recombinant Proteins; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D049452:Green Fluorescent Proteins; D011078:Polychlorinated Biphenyls; D008156:Luciferases