Lignification and related enzymes in Glycine max root growth-inhibition by ferulic acid.
Changes in soluble and cell wall bound peroxidase (POD, EC 1.11.1.7) activity, phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity, and lignin content in roots of ferulic acid-stressed soybean (Glycine max (L.) Merr.) seedlings and their relationships with root growth were investigated. Three-day-old soybean seedlings were cultivated in half-strength Hoagland nutrient solution containing 1.0 mM ferulic acid for 24-72 hr. Length, fresh weight, and dry weight of roots decreased, while soluble and cell wall bound POD activity, PAL activity, and lignin content increased after ferulic acid treatment. These enzymes probably participate in root growth reduction in association with cell wall stiffening related to the formation of cross-linking among cell wall polymers and lignin production.
dos Santos
Wanderley Dantas
WD
Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo, 5790, Maringá, PR, 87020-900, Brazil.
Ferrarese
Maria de Lourdes L
Mde L
Finger
Aline
A
Teixeira
Aline C N
AC
Ferrarese-Filho
Osvaldo
O
D001835:Body Weight; D002473:Cell Wall / Q000737:chemistry; D003373:Coumaric Acids / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D036226:Seedlings / Q000254:growth & development / Q000502:physiology; D013025:Glycine max; D013997:Time Factors
D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D008031:Lignin; C004999:ferulic acid; D010544:Peroxidases; D010650:Phenylalanine Ammonia-Lyase