Determination of lignans in human plasma by liquid chromatography with coulometric electrode array detection.
The presence of mammalian lignans, mainly enterolactone, in human plasma has been related to lower incidence of certain cancers and cardiovascular disease. The plant lignans secoisolariciresinol and matairesinol have been reported to be precursors of mammalian lignans, but recently other plant lignans relatively abundant in the diet have also been identified as precursors. To evaluate the importance and contribution of these new dietary precursors to the mammalian lignan formation in vivo, metabolic studies in human subjects must be carried out. For this purpose a method based on high-performance liquid chromatography using coulometric electrode array detection for the simultaneous determination of nine plant lignans and two mammalian lignans in human plasma was developed, validated, and shown to fulfill the reliability criteria.
Institute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center, and Division of Clinical Chemistry, University of Helsinki, Biomedicum, P.O. Box 63, 00014, Helsinki, Finland. jose.penalvo@helsinki.fi
José L
JL
Peñalvo
Tarja
T
Nurmi
Kati
K
Haajanen
Nawaf
N
Al-Maharik
Nigel
N
Botting
Herman
H
Adlercreutz
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D006801:Humans; D017705:Lignans / Q000097:blood*; D008322:Mammals; D015394:Molecular Structure; D010936:Plant Extracts; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017705:Lignans; D010936:Plant Extracts