Incidence of extended-spectrum beta-lactamases in clinical isolates of the family Enterobacteriaceae in a pediatric hospital.
The incidence of extended-spectrum beta-lactamases (ESBLs) was analyzed in Enterobacteriaceae population circulating in the Upper Silesian Child and Mother Health Center in Katowice (USC&MHC). Altogether 1164 clinical specimens, collected from children hospitalized in 8 different hospital units of USC&MHC were investigated. Five hundred and eighty-five clinical isolates of the family Enterobacteriaceae were identified in specimens collected from 403 patients. Two hundred and twenty-nine Enterobacteriaceae strains (39%) isolated from 162 patients were found to be putative ESBL producers as revealed by double-disc synergy (DDS) test. ESBL activity was the most prevalent in the population of Klebsiella pneumoniae (77%), followed by Klebsiella oxytoca (50%), Serratia marcescens (43%), Escherichia coli (30%), Enterobacter spp. (18%) and Proteus mirabilis (12%). ESBL producers demonstrated also wide resistance to the non-beta-lactam antimicrobial co-trimoxazole (93%) and the aminoglycosides netilmicin (88%), gentamicin (84%) and amikacin (79%).
Department of Microbiology, Medical University of Silesia, Katowice. hradosz@slam.katowice.pl
Radosz-Komoniewska
RK
Halina
Gniadkowski
G
Marek
Rogala-Zawada
RZ
Danuta
Nowakowska
N
Maria
Rudy
R
Maria
Wiechuła
W
Barbara
Martirosian
M
Gayane
D002648:Child; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D015994:Incidence; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000032:analysis*
D001618:beta-Lactamases