Postischemic mild hypothermia reduces neurotransmitter release and astroglial cell proliferation during reperfusion after asphyxial cardiac arrest in rats.
The present study investigated whether postischemic mild hypothermia attenuates the ischemia-induced striatal glutamate (GLU) and dopamine (DA) release, as well as astroglial cell proliferation in the brain. Anesthetized rats were exposed to 8 min of asphyxiation, including 5 min of cardiac arrest. The cardiac arrest was reversed to restoration of spontaneous circulation (ROSC), by brief external heart massage and ventilation within a period of 2 min. After the insult and during reperfusion, the extracellular glutamate and dopamine overflow increased to, respectively, 3000% and 5000% compared with the baseline values in the normothermic group and resulted in brain damage, ischemic neurons and gliosis. However, when hypothermia was induced for a period of 60 min after the insult and restoration of spontaneous circulation, the glutamate and dopamine overflows were not significantly different from that in the sham group. Histological analysis of the brain showed that postischemic mild hypothermia reduced brain damage, ischemic neurons, as well as astroglial cell proliferation. Thus, postischemic mild hypothermia reduces the excitotoxic process, brain damage, as well as astroglial cell proliferation during reperfusion. Moreover, these results emphasize the trigger effect of dopamine on the excitotoxic pathway.
Department of Critical Care Medicine and Cerebral Resuscitation Research Group, van de Vrije Universiteit Brussel, Laarbeeklaan 101, Brussels B-1090, Belgium. ndphiis@az.vub.ac.be
S
S
Hachimi-Idrissi
A
A
Van Hemelrijck
A
A
Michotte
I
I
Smolders
S
S
Sarre
G
G
Ebinger
L
L
Huyghens
Y
Y
Michotte
D000818:Animals; D001237:Asphyxia / Q000378:metabolism*; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D006323:Heart Arrest / Q000378:metabolism*; D007036:Hypothermia, Induced / Q000379:methods*; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D013997:Time Factors
D018377:Neurotransmitter Agents