The preparation of side chain functionalized analogues of coenzyme Q for protein conjugation studies.
The synthesis of two analogues of CoQ (10 and 13) suitable for conjugation to a peptide or protein, and hence the development of an ELISA immunoassay, is presented. These analogues were synthesized from the protected quinone, 1-bromo-2-methyl-3,4,5,6-tetramethoxybenzene (1), itself prepared from commercially available CoQ-0 (3). Model coupling studies of one of the analogues (10) to N-acetyl-L-lysine methyl ester and a lysine containing dipeptide (N-acetyl-glycine-L-lysine methyl ester) were also undertaken as a first step to monoclonal antibody production.
Daines
Alison M
AM
Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
Abell
Andrew D
AD
D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*
D011506:Proteins; D014451:Ubiquinone