S19-mRNA expression in squamous cell carcinomas of the upper aerodigestive tract.
BACKGROUND
The differential display method showed altered expression of ribosomal protein S19 gene in human head and neck squamous cell carcinoma (HNSCC) cell lines.


METHODS
To verify these results, RT-PCR analysis was carried out in 18 HNSCC and 17 benign epithelial cell lines as well as 30 HNSCC and 8 reference tissue samples. In the HNSCC cells S19 mRNA expression was significantly reduced as compared to benign epithelial cells.


RESULTS
Change of the S19 gene expression in surgical samples was detectable but not significant although the histopathological grading of the HNSCC biopsies correlated significantly with the S19 mRNA expression levels. The expression of ribosomal protein S6 and S14 genes were additionally analyzed using the same methods.


CONCLUSIONS
High correlation was found between the expression of S6/S14 and S19 suggesting that changes in S19 gene expression might be the result of loss of ribosomes in HNSCC cells.
Sengpiel
Verena
V
Department of Otolaryngology, Head and Neck Surgery, University of Marburg, Deutschhausstr. 3, 35037 Marburg, Germany.
Rost
Tobias
T
Görögh
Tibor
T
Rathcke
Immo O
IO
Werner
Jochen A
JA
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019893:Ribosomal Protein S6 Kinases / Q000096:biosynthesis / Q000235:genetics; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors
D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C051065:THRSP protein, human; D014157:Transcription Factors; C040343:ribosomal protein S19; D019893:Ribosomal Protein S6 Kinases