A distinctive class of spermidine synthase is involved in chilling response in rice.
A cDNA for a putative 42 kD spermidine synthase (OsSPDS2) was cloned from rice. The deduced OsSPDS2 sequence showed highest similarity with Arabidopsis AtSPDS3. Phylogenetic analysis revealed that OsSPDS2 and AtSPDS3 form a distinctive subclass in the spermidine synthase family in plants. OsSPDS2 mRNA accumulated in roots during long term exposure to chilling temperature (12 degrees C). In contrast, no such induction of the paralogous OsSPDS1 was observed during the chilling treatment. ABA treatment up-regulated OsSPDS2, whereas salt stress did not change OsSPDS2 levels significantly. Data suggested a distinct function of OsSPDS2 in chilling response in rice.
Winter Stress Laboratory, Department of Low Temperature Sciences, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Toyohira-ku, Sapporo, Japan. rzi@affrc.go.jp
Ryozo
R
Imai
Asghar
A
Ali
Habibur R
HR
Pramanik
Kentaro
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Nakaminami
Naoki
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Sentoku
Hideki
H
Kato
D000040:Abscisic Acid / Q000494:pharmacology; D000064:Acclimatization / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D011436:Spermidine Synthase / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D011436:Spermidine Synthase