Structural analysis of mycobacterial lipoglycans.
Mycobacterium tuberculosis, the causative agent of tuberculosis, is one of the most effective human pathogens. The mycobacterial cell envelope contains lipoglycans, and of particular interest is lipoarabinomannan (LAM), one of the most potent mycobacterial immunomodulatory molecules. The importance of lipoarabinomannan (LAM) in the immunopathogenesis of tuberculosis has incited structural studies on this molecule to (1) establish a precise structural model of the molecule and (2) decipher the structure/function relationships. In recent years, we have focused on the two domains essential for LAM biologic activities: the mannosyl-phosphatidyl-myo-inositol anchor and the caps. We review here the recent procedures developed for the structural analysis of these domains.
Nigou
Jérôme
J
Department of Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205, Route de Narbonne, 31077 Toulouse Cedex 4, France. jerome.nigou@ipbs.fr
Gilleron
Martine
M
Brando
Thérèse
T
Puzo
Germain
G
D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D009163:Mycobacterium bovis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000737:chemistry; D010759:Phosphorus Isotopes; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008070:Lipopolysaccharides; D010716:Phosphatidylinositols; D010759:Phosphorus Isotopes; D005990:Glycerol; D008358:Mannose