Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection.
Gene deletion analysis is a powerful tool for resolving the contributions of individual open reading frames to the physiology of cells. Analysis of deletion phenotypes in conjunction with a specific pathway reporter can identify constituents of a physiological pathway and reveal potential effectors that regulate the pathway by quantifying the phenotypic responses of the mutant cells. This article describes a high-throughput method of analyzing a yeast gene deletion library for novel G-protein signaling modulators using a yeast pheromone pathway-specific reporter.
Chasse
Scott A
SA
Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, 27599-7260, USA.
Dohlman
Henrik G
HG
D003625:Data Collection; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015723:Gene Library; D005800:Genes, Fungal; D017930:Genes, Reporter; D016366:Open Reading Frames; D010675:Pheromones / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015398:Signal Transduction; D001616:beta-Galactosidase
D010675:Pheromones; D043562:Receptors, G-Protein-Coupled; D001616:beta-Galactosidase