Atomic force microscopy studies on DNA structural changes induced by vincristine sulfate and aspirin.
We report that atomic force microscopy (AFM) studies on structural variations of a linear plasmid DNA interact with various concentrations of vincristine sulfate and aspirin. The different binding images show that vincrinstine sulfate binding DNA chains caused some loops and cleavages of the DNA fragments, whereas aspirin interaction caused the width changes and conformational transition of the DNA fragments. Two different DNA structural alternations could be explained by the different mechanisms of the interactions with these two components. Our work indicates that the AFM is a powerful tool in studying the interaction between DNA and small molecules.
Zhu
Yi
Y
Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China.
Zeng
Hu
H
Xie
Jianming
J
Ba
Long
L
Gao
Xiang
X
Lu
Zuhong
Z
D001241:Aspirin / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure*; D015252:Deoxyribonucleases, Type II Site-Specific; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000187:drug effects / Q000648:ultrastructure*; D014750:Vincristine / Q000494:pharmacology*
D014750:Vincristine; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; D001241:Aspirin