Tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats.
The treatment of persistent bone defects has encouraged the search for proper techniques or bone substitutes. In Dentistry, a common problem in the treatment of periodontal bone defects is the growth of tissues within the lesion, such as the junctional epithelium, which impair regeneration of these tissues. Guided tissue regeneration (GTR), based on the separation of the tissues by means of membranes or barriers, was developed in an attempt to improve periodontal regeneration. The aim of this study was to histologically evaluate the tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats. The study periods were 1, 3, 7, 15, 30 and 60 days after implantation. Analysis of the histological sections demonstrated a moderate to intense inflammatory response at 1 and 3 days, moderate at 7 and 15 days, and almost absent at 30 and 60 days. Resorption of the membrane began 15 days after implantation, and at 60 days only remnants could be detected in some animals. We concluded that the demineralized bovine cortical bone membrane was well tolerated by the tissues and is completely resorbed after 30-60 days by mononuclear cells and multinucleated giant cells, which disappear upon completion of the process.
Department of Endodontics, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil.
Rodrigo Cardoso
RC
de Oliveira
Renato
R
Menezes
Tânia Mary
TM
Cestari
Eulázio Mikio
EM
Taga
Rumio
R
Taga
Marília Afonso Rabelo
MA
Buzalaf
José Mauro
JM
Granjeiro
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001706:Biopsy; D001862:Bone Resorption / Q000473:pathology; D016025:Bone Transplantation / Q000379:methods* / Q000473:pathology; D002417:Cattle; D015726:Giant Cells / Q000473:pathology; D016591:Histocytological Preparation Techniques; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic; D009504:Neutrophils / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery*; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D008567:Membranes, Artificial