Analysis of erythrocyte and platelet membrane proteins in various forms of beta-thalassemia.
Major membrane proteins have been quantitatively analyzed in erythrocytes and platelets from patients with homozygous (splenectomized and non-splenectomized) and heterozygous forms of beta-thalassemia depending on severity of clinical manifestation of this disease. Quantitative analysis of erythrocyte membrane proteins revealed increase in alpha- and beta-spectrin. (In non-splenectomized patients with homozygous beta-thalassemia the amount of this protein was lower than in corresponding controls.) Besides spectrin, the increase of 2.1-2.3 fractions of ankyrin, and the decrease of band 3 protein (anion-transport protein), 4.1, palladin, and glyceraldehyde-3-phosphate dehydrogenase were also found. Analysis of major platelet membrane proteins revealed significant increase in gelsolin. This increase was found in all forms of beta-thalassemia irrespective of gender. Significant changes in platelet membrane protein fractions were found in patients (especially non-splenectomized) with homozygous beta-thalassemia. These included significant decrease in myosin, profilin, and gamma-actin and increase in actin-binding protein in both male and female patients. The content of other protein fractions (alpha-actinin, tubulin, tropomyosin) remained unchanged. Changes in protein fractions of erythrocytes and platelets correlated with severity of clinical manifestation of the disease.
Alekperova
G A
GA
Azerbaijan Medical University, Baku 370022, Azerbaijan.
Orudzhev
A G
AG
Javadov
S A
SA
D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000293:Adolescent; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D017487:Ankyrins / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002648:Child; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D018260:Gelsolin / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D051304:Profilins / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism; D014335:Tropomyosin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D017086:beta-Thalassemia / Q000097:blood / Q000378:metabolism*
D000199:Actins; D001457:Anion Exchange Protein 1, Erythrocyte; D017487:Ankyrins; D003598:Cytoskeletal Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; C412722:PALLD protein, human; D010750:Phosphoproteins; D051304:Profilins; D014335:Tropomyosin; D014404:Tubulin; D000185:Actinin; D013049:Spectrin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009218:Myosins