Cricoid regeneration using in situ tissue engineering in canine larynx for the treatment of subglottic stenosis.
The purpose of the present study was to evaluate the efficacy of cricoid regeneration via in situ tissue engineering in a canine larynx for the treatment of subglottic stenosis. As the tissue scaffold, a Marlex mesh tube coated by collagen sponge was used for a rigid airway framework and for tissue regrowth around the tube. On 5 dogs, the larynx was exposed and the anterior third of the cricoid cartilage was resected. The tube was anastomosed to the lower edge of the thyroid cartilage and to the first tracheal cartilage. By postoperative endoscopic examination at 3 to 7 months, no airway obstruction was observed in any of the dogs. There was granulation tissue in 2 dogs and slight mesh exposure in 1 dog, but they were asymptomatic. Confluent regeneration of the epithelium over the scaffold and good incorporation of the scaffold mesh into the host tissue were observed after surgery.
Omori
Koichi
K
Department of Otolaryngology, Fukushima Medical School, School of Medicine, Fukushima City (Omori), Japan.
Nakamura
Tatsuo
T
Kanemaru
Shinichi
S
Kojima
Hisayoshi
H
Magrufov
Akhmar
A
Hiratsuka
Yasuyuki
Y
Shimizu
Yasuhiko
Y
D000818:Animals; D003413:Cricoid Cartilage / Q000502:physiology / Q000601:surgery*; D004285:Dogs; D006097:Granulation Tissue / Q000502:physiology; D006801:Humans; D007829:Laryngostenosis / Q000601:surgery*; D008567:Membranes, Artificial; D011126:Polypropylenes; D019736:Prostheses and Implants; D012038:Regeneration; D013526:Surgical Mesh; D023822:Tissue Engineering
D008567:Membranes, Artificial; D011126:Polypropylenes