Kinetics of biotransformation of 2,4-dichlorophenol using UASB-reactor.
Chlorophenol compounds are environmental pollutants that are both anthropogenic and xenobiotics. Some of these chemicals are carcinogens and are both toxic to a number biochemical processes. Biotransformation of 2,4-dichlorophenol (2,4-DCP) was studied in the presence of glucose on an upflow anaerobic sludge blanket reactor (UASB) using mixed culture. A continuously operated UASB reactor was employed using mixed synthetic wastewater. Results obtained from the 1.8 L volume capacity UASB reactor were subjected to kinetic evaluation constants. Results indicate that the degradation of 2,4-DCP in the presence of glucose was strongly influenced by the concentration of the compound. High degradation levels were observed when the concentration of 2,4-DCP was in the range of 50-150 mg L(-1). Concentrations of 2,4-DCP above 160 mg L(-1) were toxic to microbes even in the presence of glucose. The maximum degradation of 2,4-DCP was found to be 70.4% when initial concentration of 2,4-DCP was 124 mg L(-1) and glucose concentration of 500 mg L(-1) at hydraulic retention time of 13.2 hr. The biodegradation followed first order reaction kinetics with a rate constant (K) of 0.67, Vmax of 0.244 kg m(-3) day(-1), Ks of 0.117 kg m(-3) day(-1) and correlation coefficient of 0.766.
Atuanya
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Department of Microbiology, University of Benin, Benin City, Nigeria. atuei@uniben.edu.ng
Chakrabarti
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D000693:Anaerobiosis; D000871:Anthelmintics; D019149:Bioreactors; D001711:Biotransformation; D002733:Chlorophenols / Q000493:pharmacokinetics*; D007700:Kinetics
D000871:Anthelmintics; D002733:Chlorophenols; C004762:2,4-dichlorophenol