Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique.
A DNA array has been fabricated on glass substrates, which enables high-throughput analysis of single-base mismatches. In this work, microfabrication-compatible plasma-polymerization (PP) method was used for immobilizing probe DNAs to study the hybridization behavior by changing surface properties. The immobilization matrix consisting of 35 A of PP layer, applied additionally on the streptavidin absorbed hexamethyldisiloxane (HMDS)-PP layer, was constructed on the substrates to anchor biotinylated DNA probes onto the surface. The hydrophobic immobilization matrix was considered to enhance hybridization accuracy and efficiency, compared with its hydrophilic acetonitrile-PP layers. The oligonucleotide arrays fabricated on HMDS-PP surface were shown to be effective in detection of single nucleotide polymorphisms (SNPs) of ApoE gene.
Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 4, 1-1-1 Higashi, Tsukuba-city, Ibaraki 305-8562, Japan.
Hirotaka
H
Miyachi
Kazunori
K
Ikebukuro
Kazuyoshi
K
Yano
Hiroyuki
H
Aburatani
Isao
I
Karube
D001057:Apolipoproteins E / Q000032:analysis / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D008420:Manufactured Materials; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D001057:Apolipoproteins E; D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D011108:Polymers