Differential distribution of synaptic endings containing glutamate, glycine, and GABA in the rat dorsal cochlear nucleus.
The dorsal cochlear nucleus (DCN) integrates the synaptic information depending on the organization of the excitatory and inhibitory connections. This study provides, qualitatively and quantitatively, analyses of the organization and distribution of excitatory and inhibitory input on projection neurons (fusiform cells), and inhibitory interneurons (vertical and cartwheel cells) in the DCN, using a combination of high-resolution ultrastructural techniques together with postembedding immunogold labeling. The combination of ultrastructural morphometry together with immunogold labeling enables the identification and quantification of four major synaptic inputs according to their neurotransmitter content. Only one category of synaptic ending was immunoreactive for glutamate and three for glycine and/or gamma-aminobutyric-acid (GABA). Among those, nine subtypes of synaptic endings were identified. These differed in their ultrastructural characteristics and distribution in the nucleus and on three cell types analyzed. Four of the subtypes were immunoreactive for glutamate and contained round synaptic vesicles, whereas five were immunoreactive for glycine and/or GABA and contained flattened or pleomorphic synaptic vesicles. The analysis of the distribution of the nine synaptic endings on the cell types revealed that eight distributed on fusiform cells, six on vertical cells and five on cartwheel cells. In addition, postembedding immunogold labeling of the glycine receptor alpha1 subunit showed that it was present at postsynaptic membranes in apposition to synaptic endings containing flattened or pleomorphic synaptic vesicles and immunoreactive for glycine and/or GABA on the three cells analyzed. This information is valuable to our understanding of the response properties of DCN neurons.
Rubio
Maria E
ME
Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269-4156, USA. maria.rubio@uconn.edu
Juiz
José M
JM
D000818:Animals; D017626:Cochlear Nucleus / Q000166:cytology*; D003712:Dendrites / Q000145:classification / Q000378:metabolism / Q000648:ultrastructure; D018698:Glutamic Acid / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods; D008959:Models, Neurological; D009433:Neural Inhibition; D009474:Neurons / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005998:Glycine