Segmental isotopic labeling for structural biological applications of NMR.
This chapter describes the preparation of precursor domains for the formation of multidomain segmentally labeled proteins by protein ligation.
New York Structural Biology Center, New York, NY, USA.
David
D
Cowburn
Alexander
A
Shekhtman
Rong
R
Xu
Jennifer J
JJ
Ottesen
Tom W
TW
Muir
D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D015202:Protein Engineering; D011522:Protons; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D011522:Protons; D011993:Recombinant Fusion Proteins; D003545:Cysteine