The effect of osteogenic growth factors on bone growth into a ceramic filled defect around an implant.
Currently available synthetic bone substitutes perform poorly compared to autograft. It is hoped that by adding osteogenic growth factors to the materials, new bone formation could be increased and the clinical outcome improved. In this study, IGF-1, bFGF and TGFbeta1, alone and in combination, were absorbed onto a carrier of beta-tricalcium phosphate (betaTCP) and implanted into a defect around a hydroxyapatite-coated, stainless steel implant in the proximal tibia of rat in a model of revision arthroplasty. Animals were sacrificed at 6 and 26 weeks for routine histology and histomorphometry and mechanical push out tests. The results show that only bFGF had a significant effect on ceramic resorption. The groups that received bFGF and bFGF in combination with TGFbeta1 had smaller and fewer betaTCP particles remaining in the defect at 6 and 26 weeks. No growth factor combination significantly enhanced new bone formation or the mechanical strength of the implant. These results indicate that, of the growth factors tested, only bFGF had any beneficial effect on the host response to the implant, perhaps by delaying osteoblast differentiation and thereby prolonging osteoclast access to the ceramic.
Clarke
S A
SA
Orthopaedic Research Unit, University of Cambridge, Box 180, Addenbrooke's Hospital, Hill's Road, Cambridge CB2 2QQ, UK.
Brooks
R A
RA
Lee
P T H
PT
Rushton
N
N
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002130:Calcium Phosphates / Q000032:analysis; D002516:Ceramics; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D020007:Implants, Experimental; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000601:surgery*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D002130:Calcium Phosphates; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C485817:beta-tricalcium phosphate; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I