Mutational analysis of the fruA promoter region demonstrates that C-Box and 5-base-pair elements are important for expression of an essential developmental gene of Myxococcus xanthus.
Myxococcus xanthus uses extracellular signals during development to regulate gene expression. C-signaling regulates the expression of many genes induced after 6 h into development. FruA is a protein that is necessary for cells to respond to C-signaling, but expression of the fruA gene does not depend on C-signaling. Yet the fruA promoter region has a C box and a 5-bp element, similar to the promoter regions of several C-signal-dependent genes, where these sequences are crucial. Here, we show that the C box and 5-bp elements are important for expression of fruA, demonstrating for the first time that these sequences play a role in the expression of a gene that does not depend on C-signaling and is required for M. xanthus development.
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
D
D
Srinivasan
Lee
L
Kroos
D000222:Adaptation, Physiological; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D020043:Genes, Essential; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development*; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C104848:FruA protein, Myxococcus xanthus; D014157:Transcription Factors; D001616:beta-Galactosidase