[Effect of exogenous collagen on cell function in construction of artificial biotendon in vitro].
OBJECTIVE
To investigate the influence of the exogenous collagen on the function of cells in construction of artificial biotendon.


METHODS
Three materials including human hair, carbon fiber (CF) and polyglycolic acid (PGA) were combined with exogenous collagen and co-cultured with standard transferred human embryonic tenocytes at a concentration of 3 x 10(6)/mm3 in vitro. The cell number and morphology were observed under inverted microscope and scanning electron microscope after 2 hours, 3 days and 5 days.


RESULTS
In the artificial biotendon combined with collagen, the cells concentrated around the materials and the cells adhering to the materials turned into round after 2 hours. After 3 days, the adhering cells increased. After 5 days, the shape of the cells changed from round to spindle.


CONCLUSIONS
Exogenous collagen will facilitate the cells to adhere onto materials and proliferate.
Fang
Yue
Y
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu Sichuan, PR China.
Yang
Zhiming
Z
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002244:Carbon; D000077482:Carbon Fiber; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D018920:Coculture Techniques; D024042:Collagen Type I / Q000494:pharmacology*; D004622:Embryo, Mammalian; D006197:Hair; D006801:Humans; D011100:Polyglycolic Acid; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D000077482:Carbon Fiber; D024042:Collagen Type I; D011100:Polyglycolic Acid; D002244:Carbon