Adenoviral mediated transfer of TIMP-3 partially prevents glutamate-induced cell death in primary cultured cortical neurons of the rat.
Metalloproteinases and tissue inhibitor of metalloproteinases (TIMPs) are associated with tissue reorganization after injury. The up-regulation of TIMP-3 has recently been found in ischemic brain although its functional implications are unclear. In this study we show that overexpression of TIMP-3 by adenovirus-mediated gene transfer partially protects neurons against excitotoxic death induced by glutamate in culture. The partial neuroprotection afforded by TIMP-3 has implications for our understanding of the physiological role of TIMP-3 in the normal and damaged central nervous system.
Department of Pharmacology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, PR China.
Rongbiao
R
Pi
Wei
W
Yin
Suqiu
S
Zheng
Pengxin
P
Qiu
Jian
J
Zhou
Wei
W
Guo
Tao
T
Su
Guangmei
G
Yan
D000256:Adenoviridae; D000818:Animals; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000821:virology; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D018698:Glutamic Acid / Q000633:toxicity*; D007150:Immunohistochemistry / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000821:virology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000502:physiology*; D014162:Transfection / Q000379:methods
D019717:Tissue Inhibitor of Metalloproteinase-3; D018698:Glutamic Acid