[Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation].
This paper studied the chitinase production of Beauveria bassiana Bb174 under solid state fermentation condition. The optimal medium consisted of wheat bran and silkworm chrysalis at the ratio of 4:1, supplemented with 1 g peptone L(-1) as nitrogen source and some other mineral nutrients. The enzyme activity reached 126 units per gram dry medium after cultured for 2 days at 28 degrees C and natural pH by inoculated 3 ml spore suspension into this medium. The optimal temperature and pH for chintinase production were 40 degrees C and 5.0, respectively. The temperature to lose 50% activity of the enzyme was 48 degrees C after incubated at 30-70 degrees C for 1 h. The enzyme was stable at 30-40 degrees C and pH 4-6, and the Km and Vmax values were 0.52 mg x ml(-1) and 0.7 deltaE680 x h(-1), respectively.
Department of Biology Science and Technology, Hefei College, Hefei 230022, China. jzhang1111@yahoo.com
Jie
J
Zhang
Jingmin
J
Cai
Ke
K
Wu
Shengxian
S
Jin
Renrui
R
Pan
Meizhen
M
Fan
D001203:Ascomycota / Q000201:enzymology* / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D013172:Spores, Fungal / Q000201:enzymology
D002688:Chitinases