An agonist human ICOS monoclonal antibody that induces T cell activation and inhibits proliferation of a myeloma cell line.
The inducible costimulator (ICOS), the third member of the CD28/CD152 receptor family, is an important costimulatory molecule during the immune response. In this study, a functional anti-human ICOS MAb 2C7 was obtained. The specificity of this MAb was verified by flow cytometry, Western blotting, and competition with anti-ICOS MAb ISA3. This MAb could well recognize ICOS molecule expressed on activated T cells and induce the activation as well as proliferation of T cells prestimulated by anti-human CD3 MAb. Furthermore, we found that MAb 2C7 could induce the growth arrest of XG2 cells, a human multiple myeloma cell line, which abnormally expressed ICOS molecule, and led to its apoptosis after 48 h of treatment. This functional anti-human ICOS MAb provides a valuable tool for further study of biological functions as well as signal transduction of ICOS/GL50.
Deng
Zhong-Bin
ZB
Institute of Biotechnology, Soochow University, Suzhou, China.
Zhu
Wei
W
Lu
Chang-Ming
CM
Shi
Qin
Q
Ju
Song-Gunag
SG
Ma
Hong-Bing
HB
Xu
Yin
Y
Zhang
Xue-Guang
XG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology*; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D000939:Epitopes / Q000276:immunology; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D000939:Epitopes; C556692:ICOS protein, human; C556694:Icos protein, mouse; D060889:Inducible T-Cell Co-Stimulator Protein