Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain.
A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I alpha chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immuno-histochemistry using SLA class I alpha chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I alpha chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.
Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
Wei-Ran
WR
Tang
Nobutaka
N
Kiyokawa
Tomoko
T
Eguchi
Jun
J
Matsui
Hisami
H
Takenouchi
Daisuk
D
Honma
Hiroshi
H
Yasue
Shin
S
Enosawa
Kenichi
K
Mimori
Mitsuko
M
Itagaki
Tomoko
T
Taguchi
Yohko U
YU
Katagiri
Hajime
H
Okita
Hiroshi
H
Amemiya
Junichiro
J
Fujimoto
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification*; D019556:COS Cells; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007150:Immunohistochemistry; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen