[Expression of PDCD5 in tissues of normal cervix, CIN I-III and cervical cancer].
OBJECTIVE
To investigate the expression of PDCD5 in tissues of normal cervix, CIN I-III, cervical cancer and explore the relationship between PDCD5 and cervical cancer.


METHODS
After we defined the most fitful condition, tissues from 18 cases of normal cervix, 19 of CIN I, 18 of CIN II, 20 of CIN III and 18 of cervical cancer were defined by indirect immunohistochemical technique. Positive expression rates and intensity of PDCD5 protein were investigated by observing under microscope and analyzing with computer imaging technique. The results were analyzed with one-way anova.


RESULTS
The results of immunohistochemical staining showed that the percentage of strong positive cells in normal cervical tissue and CIN I were significant higher than those of CIN II, CIN III and cervical cancer. On the whole of the condition of immunohistochemical staining, the expressions of PDCD5 were downregulated along the progression of cervical atypical epithelia, but that in CIN I was upregulated. The ODs of normal cervix,CIN I-III,cervical cancer were 0.322, 0.366, 0.287, 0.252, and 0.206 respectively. The intensity of each group showed obvious differences.


CONCLUSIONS
We found that the expression of PDCD5 was upregulated in CIN I and downregulated in CIN II, CIN III and cervical cancer. It suggests that PDCD5 is an important apoptosis regulating factor in the occurrence of cervical cancer.
Department of Obstetric and Gynecology, Peking University First Hospital, Beijing 100034, China. xyxlzh44542@sina.com
Zhao-hui
ZH
Liu
Dai
D
Zhang
Ke-min
KM
Li
Qin-ping
QP
Liao
D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis*; D002584:Cervix Uteri / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human