Effects of fatty acid-free bovine serum albumin and fetal calf serum supplementing repair cultures on pre- and post-warm viability of biopsied bovine embryos produced in vitro.
The objective of this study was to investigate the influence of fatty acid-free bovine serum albumin (BSA) or fetal calf serum (FCS) on the re-expansion of biopsied blastocysts and post-warm viability of subsequently vitrified embryos. Firstly, blastocysts produced in vitro were biopsied at Day 7 and cultured to allow repair in TCM199 with 0.3% BSA or 5% FCS for 24 h. The re-expansion rates and mean total numbers of cells of the re-expanded embryos after the repair culture with BSA were almost the same as that with FCS. Secondly, after biopsied embryos were similarly cultured for repair with BSA or FCS, re-expanded embryos were selected for vitrification. After warming and exposure to 0.5 M sucrose with 20% FCS in mPBS, the embryos were cultured in TCM199 with 5% FCS for 24 h. The re-expansion rate and mean total number of cells in re-expanded blastocysts in the BSA treatment group (97.4 +/- 2.9% and 106 +/- 42) was significantly higher than that in the FCS treatment group (51.6 +/- 9.1% and 61 +/- 38), respectively (P<0.05 and P<0.01). In conclusion, both FCS and BSA supplementation can be useful for repairing cultures of bovine biopsied blastocysts; but, compared with BSA supplementation, FCS supplementation during repair culture reduces the post-warm viability of biopsied and subsequently vitrified embryos.
Yotsushima
Kenji
K
Toyama Prefectural Agricultural Research Center, Livestock Experiment Station, Japan. kenji.yotsushima@pref.toyama.lg.jp
Sakaguchi
Minoru
M
Shimizu
Manabu
M
Okimura
Tomoko
T
Izaike
Yoshiaki
Y
D000818:Animals; D001706:Biopsy; D001757:Blastomeres / Q000166:cytology* / Q000187:drug effects*; D002417:Cattle; D002470:Cell Survival; D015925:Cryopreservation / Q000662:veterinary; D046149:Embryo Culture Techniques / Q000662:veterinary*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005307:Fertilization in Vitro / Q000662:veterinary; D005326:Fetal Proteins / Q000494:pharmacology*; D006358:Hot Temperature; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D005230:Fatty Acids, Nonesterified; D005326:Fetal Proteins; D012710:Serum Albumin, Bovine