[Studies on the changes in the interleukin-13 expression and on activator protein-1 activity in rat pulmonary tissue with acute lung injury induced by endotoxin].
OBJECTIVE
To investigate the changes in plasma level of interleukin-13 (IL-13) and the changes in the pulmonary IL-13 mRNA content and the pulmonary activator protein-1 (AP-1) activity of the rats inflicted with acute lung injury (ALI) induced by lipopolysaccharide (LPS), so as to explore the relationship between IL-13 expression and AP-1 activity.


METHODS
One hundred and twenty Wistar rats were employed in the study and were randomly divided into A (2 mg/kg), B (4 mg/kg), C (6 mg/kg) and D (8 mg/kg) groups according to different dosage of LPS administration and a control group (NS group) at each observing time point. The rats were observed at 1, 2, 4 and 6 postburn hours (PBHs) and every 6 rats were deployed in every group and each time points. A model of systemic inflammatory response syndrome-acute lung injury (SIRS-ALI) was replicated in Wistar rats. The plasma content of IL-13 was assayed by enzyme-linked immunosorbent assay (ELISA), and the pulmonary tissue content of IL-13 mRNA and AP-1 activity by reverse transcriptase-polymerase chain reaction (RT-PCR) and electrophoretic mobility shift assays (EMSA).


RESULTS
The plasma content of IL-13, pulmonary content of IL-13 mRNA and AP-1 activity increased simultaneously after LPS administration. All the above indices were significantly different statistically between the LPS groups and the control group (P < 0.05 - 0.01). The plasma level of IL-13 and pulmonary tissue mRNA content and AP-1 activity in A, B, C and D groups were increased significantly with peak levels at 2 PBHs.


CONCLUSIONS
The pulmonary AP-1 activity increased with the enhanced expression of IL-13, which was related to the development of SIRS-ALI.
Institute of Respiratory Disease, Xinqiao Hospital, The Third Military Medical University. Chongqing 400037, P. R. China.
Qi
Q
Li
Gui-Sheng
GS
Qian
Nan
N
Yang
Chang-Zheng
CZ
Wang
Jian-Cheng
JC
Xu
Qing
Q
Zhang
D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D018793:Interleukin-13 / Q000097:blood / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018808:Transcription Factor AP-1 / Q000032:analysis / Q000502:physiology*
D004731:Endotoxins; D018793:Interleukin-13; D018808:Transcription Factor AP-1