Establishment and characterization of metastasizing cell lines from a heterotransplanted human adenoid cystic carcinoma.
Adenoid cystic carcinoma (ACC) is a rare malignant tumor of the head and neck occurring in the salivary glands. We established a human ACC line which is serially transplantable in nude mice and designated it as KOA-1. The KOA-1 tumor doubled in 9.3 days and retained the histologic characteristics of a solid pattern of ACC even after 22 serial passages. The KOA-1 metastasized to the lung 2 months after subcutaneous transplantation into nude mice. We further established a subline of KOA-1 by an in vivo selection method and named it KOA-1L3. The KOA-1L3 doubled in 1.3 days and metastasized to the lung within 1 week after subcutaneous transplantation. These tumor lines may serve as a useful model for exploration of the biological behavior and treatment of human ACC.
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine Japan. ume@med.kobe-u.ac.jp
Masahiro
M
Umeda
Hideki
H
Komatsubara
Yasutaka
Y
Ojima
Tsutomu
T
Minamikawa
Yasuyuki
Y
Shibuya
Satoshi
S
Yokoo
Takahide
T
Komori
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology* / Q000556:secondary; D002423:Cause of Death; D002455:Cell Division; D045744:Cell Line, Tumor; D062109:Dermatologic Surgical Procedures; D005856:Germ-Free Life; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D008938:Mitosis; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured