Determination of the concentration of protein in cerebrospinal fluid with a new dye-binding method.
The protein concentration of cerebrospinal fluid (CSF) was measured on 50 samples with a new dye-binding method using Coomassie Brilliant Blue G-250 (CBB). The results of this method correlated well (r = 0.99) with those of the trichloroacetic acid-Ponceau S method (TCA-PS). Since the CBC method involves only one step, it is recommended as a simple method of determining protein concentration in CSF.
Barsanti
J A
JA
Departments of Small Animal Medicine and Pathology, University of Georgia, Athens, Georgia 30602.
Duncan
J R
JR