Direct electrochemistry of porcine purple acid phosphatase (uteroferrin).
Cyclic voltammetry of the non-heme diiron enzyme porcine purple acid phosphatase (uteroferrin, Uf) has been reported for the first time. Totally reversible one-electron oxidation responses (FeIII-FeII --> FeII-FeIII) are seen both in the absence and in the presence of weak competitive inhibitors phosphate and arsenate, and dissociation constants of these oxoanion complexes formed with uteroferrin in its oxidized state (Uf(o)) have been determined. The effect of pH on the redox potentials has been investigated in the range 3 < pH < 6.5, enabling acid dissociation constants for Uf(o) and its phosphate and arsenate complexes to be calculated.
Bernhardt
Paul V
PV
Center for Metals in Biology, Department of Chemistry, University of Queensland, Brisbane 4072, Australia. P.Bernhardt@uq.edu.au
Schenk
Gerhard
G
Wilson
Gregory J
GJ
D000135:Acid Phosphatase / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001149:Arsenates / Q000494:pharmacology; D001667:Binding, Competitive; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004579:Electron Transport; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D010084:Oxidation-Reduction; D010710:Phosphates / Q000494:pharmacology; D013552:Swine
D001149:Arsenates; D004791:Enzyme Inhibitors; D006023:Glycoproteins; D010710:Phosphates; D007501:Iron; C091137:purple acid phosphatase; D000135:Acid Phosphatase; C025657:arsenic acid