Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.
Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment.
Zeng
Rong
R
Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
Yang
Rui-Fu
RF
Shi
Mu-De
MD
Jiang
Man-Rong
MR
Xie
You-Hua
YH
Ruan
Hong-Qiang
HQ
Jiang
Xiao-Sheng
XS
Shi
Lv
L
Zhou
Hu
H
Zhang
Lei
L
Wu
Xiao-Dong
XD
Lin
Ying
Y
Ji
Yong-Yong
YY
Xiong
Lei
L
Jin
Yan
Y
Dai
Er-Hei
EH
Wang
Xiao-Yi
XY
Si
Bin-Ying
BY
Wang
Jin
J
Wang
Hong-Xia
HX
Wang
Cui-E
CE
Gan
Yong-Hua
YH
Li
Yu-Chuan
YC
Cao
Ju-Tian
JT
Zuo
Jiang-Ping
JP
Shan
Shi-Fang
SF
Xie
En
E
Chen
Song-Hua
SH
Jiang
Zhi-Qin
ZQ
Zhang
Xi
X
Wang
Yuan
Y
Pei
Gang
G
Sun
Bing
B
Wu
Jia-Rui
JR
D000818:Animals; D002522:Chlorocebus aethiops; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D010802:Phylogeny; D040901:Proteomics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
C487105:3a protein, SARS-CoV; D004220:Disulfides; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000086082:Viroporin Proteins