A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate.
An effective vaccine against Edwardsiella tarda has not been reported, one of main reasons is the variation in its serotypes. This study aimed to develop an effective vaccine against different serotypes of E. tarda. A conserved 37 kDa outer membrane protein (OMP) of E. tarda was obtained by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). It showed comprising several proteins by two-dimensional (2D) PAGE analysis and showed a high similarity to glyceraldehyde-3-phosphate dehydrogenase by N-terminal amino acid sequence analysis. Japanese flounder (Paralichthys olivaceus) vaccinated with the 37 kDa OMP was significantly protected against the infections by different serotypes of E. tarda. A specific antibody was also detected by using ELISA. This study suggests that the 37 kDa OMP is an effective potent vaccine candidate against different serotypes of E. tarda.
Department of Aquaculture, Fish Disease Laboratory, Faculty of Agriculture, Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi 783-8502, Japan.
Kenji
K
Kawai
Ying
Y
Liu
Kouhei
K
Ohnishi
Syun-Ichirou
S
Oshima
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D017124:Conserved Sequence; D020609:Edwardsiella tarda / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control*; D005399:Fishes / Q000276:immunology*; D006041:Goats / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D008969:Molecular Sequence Data; D019081:O Antigens / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D012703:Serotyping; D016019:Survival Analysis
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019081:O Antigens