The use of the cMyc epitope tag can be problematic for protein detection in Legionella pneumophila.
In various recent experiments with respect to protein secretion by L. pneumophila, protein detection based on the cMyc-tag seemed to be problematic. For one specific protein, we studied the reason for this problem in more detail by comparing protein expression in the presence and absence of the cMyc-tag. We demonstrated a decrease in the amount of mRNA transcript when the cMyc coding sequence is present. We can conclude that this epitope tag is not a preferential tag for protein detection in L. pneumophila.
De Buck
Emmy
E
Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, Leuven B-3000, Belgium.
Lebeau
Ilya
I
Van Mellaert
Lieve
L
Geukens
Nick
N
Anné
Jozef
J
Lammertyn
Elke
E
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D000939:Epitopes; D016271:Proto-Oncogene Proteins c-myc; D012329:RNA, Bacterial