Detection of trophic factor activated signaling molecules in cells by a compact fiber-optic sensor.
This paper describes a highly sensitive method to detect trophic factor activated signaling molecules in cells using a compact fiber optic biosensor. The method is demonstrated by quantitative detection of phosphorylation of signal transducers and activators of transcription 3 (STAT3) in neuroblastoma cells. A single fiber-optic probe based on total internal reflection fluorescence sensing system is used. A 405 nm diode laser is used for evanescent wave excitation of immobilized labelled analyte on the probe surface. A compact charged coupled device (CCD) based spectrometer is used for recording the fluorescence signal. The method is two orders of magnitude more sensitive than the Western blotting technique.
Kapoor
Rakesh
R
Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, 458 NSC, Buffalo, NY 14260, USA. rkapoor@acsu.buffalo.edu
Kaur
Navjot
N
Nishanth
Emmanuel T
ET
Halvorsen
Stanley W
SW
Bergey
Earl J
EJ
Prasad
Paras N
PN
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D009447:Neuroblastoma / Q000175:diagnosis / Q000378:metabolism*; D055100:Optical Fibers; D017978:Receptors, Growth Factor / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results; D050796:STAT3 Transcription Factor; D012680:Sensitivity and Specificity; D015398:Signal Transduction; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism*
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D017978:Receptors, Growth Factor; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators