Development of a monolithic silica extraction tip for the analysis of proteins.
In proteomics, pre-treatment of sample is the most important procedure to remove the matrix for interfacing with mass spectrometry (MS). Additionally, for the samples with low concentration, the process of pre-concentration is required before MS analysis. We have newly developed solid-phase extraction (SPE) tool with pipette-tip shape for purification of bio-samples of various characteristics, utilizing monolithic silica gel as medium. The monolithic silica surface was modified with a C18 phase or coated with titania phase. A C18-bonded tip and a non-modified tip were used for sample concentration, desaltination and removal of detergents from sample. A titania-coated tip was also applied for purification and concentration of phosphorylated peptides. This novel pre-treatment method using monolithic silica extraction tip is much effective and suitable for protein analysis.
Miyazaki
Shota
S
GL Sciences Inc., Iruma, Saitama, Japan. s-miyazaki@gls.co.jp
Morisato
Kei
K
Ishizuka
Norio
N
Minakuchi
Hiroyoshi
H
Shintani
Yukihiro
Y
Furuno
Masahiro
M
Nakanishi
Kazuki
K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*
D011506:Proteins; D012822:Silicon Dioxide