Gel filtration chromatography of metallothionein obtained from rabbit liver.
Metallothioneins (MTs) are known as heat-stable proteins and are able to be precipitated at 100 degrees C for 2 min. The aim of this work was to determine the content of metallothioneins and zinc in fraction after gel filtration (Sephadex G-75). The research was supposed to answer the question whether metallothioneins contain zinc ions after heat-treated cytosol. The rabbit liver cytosol was initially heat-treated to remove the heat-liable proteins and then cytosol was applied to a gel filtration column (Sephadex G-75). Metallothioneins content in each eluted fraction was measured by cadmium-hemoglobin affinity assay using the cadmium isotope (109Cd). Another experiment was conducted to further confirm whether the MTs peak contains zinc. A metal peak appeared at low molecular weight fractions. The metallothioneins isolated from rabbit liver contain zinc.
Department of Biochemistry and Molecular Biology, Medical University of Lublin.
Bolesław
B
Floriańczyk
D000818:Animals; D002850:Chromatography, Gel; D003545:Cysteine / Q000032:analysis; D003600:Cytosol / Q000737:chemistry*; D008668:Metallothionein / Q000032:analysis*; D011817:Rabbits; D015032:Zinc / Q000032:analysis*
D008668:Metallothionein; D015032:Zinc; D003545:Cysteine