Contribution of a glial glutamate transporter to GABA synthesis in the retina.
Neuronal glutamate transporters have been shown to play a role in GABA synthesis by enhancing glutamate uptake. In the present study, we have examined whether a glial glutamate transporter, GLAST, has a role in GABA synthesis in the mammalian retina. We found that the retinal GABA level was about two-fold higher in the GLAST-/- mouse retina compared to that in the wild type. Endogenous glutamate level was also increased about 2-fold in the mutant. Therefore, loss of GLAST results in a higher retinal GABA level, probably due to increased availability of its precursor, glutamate. An increase in GABAergic activity can be expected to affect trigger features such as directional selective response of neurons in the GLAST-/- mouse retina.
Sarthy
Vijay P
VP
Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. vjsarthy@northwestern.edu
Marc
Robert E
RE
Pignataro
Leonardo
L
Tanaka
Kohichi
K
D027322:Amino Acid Transport System X-AG / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005110:Extracellular Space / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009457:Neuroglia / Q000378:metabolism*; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
D027322:Amino Acid Transport System X-AG; D000596:Amino Acids; D005973:Glutamine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid