IPNV carrier Atlantic salmon growers do not express Mx mRNA and poly I:C-induced Mx response does not cure the carrier state.
Injection of infectious pancreatic necrosis virus (IPNV) in post-smolt Atlantic salmon induced a rapid and persistent expression of Mx mRNA from day 1 to at least day 11 when Mx:beta actin ratios were still at peak values of about 1.0. In contrast, an Atlantic salmon grower population, shown to be carriers of IPNV by culture of the virus from plastic adherent kidney leucocytes, showed no evidence of the expression of Mx transcripts. Nevertheless, IPNV-carrier growers showed a typical Mx response following injection of poly I:C, beginning on day 1, peaking on day 3 (mean Mx:beta actin ratio 0.82) and disappearing by day 7. Notwithstanding such treatment, IPNV continued to persist in growers as the virus could still be isolated 14 days after poly I:C injection.
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, UK.
K
K
Lockhart
S K
SK
Gahlawat
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D
Soto-Mosquera
T J
TJ
Bowden
A E
AE
Ellis
D000199:Actins / Q000378:metabolism; D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D017931:DNA Primers; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D018061:Infectious pancreatic necrosis virus / Q000276:immunology*; D064547:Myxovirus Resistance Proteins; D011070:Poly I-C / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020317:Salmo salar; D013997:Time Factors
D000199:Actins; D017931:DNA Primers; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D011070:Poly I-C