Biosynthesis of the thiamin pyrimidine: the reconstitution of a remarkable rearrangement reaction.
The conversion of 5-aminoimidazole ribonucleotide (AIR) into 4-amino-2-methyl-5-hydroxymethylpyrimidine (HMP) is a fascinating reaction on the thiamin biosynthetic pathway in bacteria and is probably the most complex unresolved rearrangement in primary metabolism. We have successfully reconstituted this reaction in a cell-free system. The E. coli thiC gene product and an additional unidentified E. coli protein are required for the reaction. In addition, SAM and nicotinamide cofactors are required for full activity. Labeling studies to determine the origin of most of the atoms in the pyrimidine are described. Based on these studies, a new mechanism for HMP formation is proposed.
Lawhorn
Brian G
BG
Baker Laboratory, Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Mehl
Ryan A
RA
Begley
Tadhg P
TP
D002474:Cell-Free System / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008968:Molecular Conformation; D011743:Pyrimidines / Q000096:biosynthesis* / Q000138:chemical synthesis* / Q000737:chemistry; D012265:Ribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors
D011743:Pyrimidines; D012265:Ribonucleotides; C024828:aminoimidazole ribotide; C012068:toxopyrimidine; D013831:Thiamine