Mouse granulocyte-macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions.
The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase cell numbers of embryos. Addition of 2 ng/ml GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1- to 2-cell parthenotes developing in vitro. However, total cell numbers were not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcriptase polymerase chain reaction revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst stage parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium.
Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk 361-763, South Korea.
Xiang-Shun
XS
Cui
Jae Yeong
JY
Lee
Seok Hwa
SH
Choi
Mo Sun
MS
Kwon
Teoan
T
Kim
Nam-Hyung
NH
Kim
D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D046508:Culture Techniques; D004558:Electric Stimulation; D010064:Embryo Implantation / Q000235:genetics; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009865:Oocytes / Q000502:physiology; D010312:Parthenogenesis; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000235:genetics; D053606:Receptors, OSM-LIF; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D051269:Sodium-Glucose Transporter 1; D013552:Swine / Q000196:embryology*
D015850:Interleukin-6; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D051269:Sodium-Glucose Transporter 1; D012710:Serum Albumin, Bovine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor