Effects of dendritic cells transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.
OBJECTIVE
To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.


METHODS
The wild-type p53 was transduced to DCs with adenovirus, and the DCs were stimulated by gastric cancer lysates. The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCs to induce efficient and specific immunological response in anti-51Cr-labeled target cells was studied. BALB/c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53DC was evaluated. Tumor-bearing mice were treated with Lywt-p53DC.


RESULTS
The surface molecules of Lywt-p53DC had a high expression of B7-1 (86.70 +/- 0.07%), B7-2 (18.77 +/- 0.08%), MHC-I (87.20 +/- 0.05%) and MHC-II (56.70 +/- 0.07%); T lymphocytes had a specific CTL lysis ability induced by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immune protection of Lywtp-53DC was obvious, the tumor diameter in Lywtp-53DC group was 3.10 +/- 0.31 mm, 2.73 +/- 0.23 mm, 3.70 +/- 0.07 mm on d 13, 16 and 19, respectively, which were smaller than control, DC, wtp53DC and LyDC group (P<0.05). Tumor growth rate in Lywtp53DC group was slower than that in other groups (P<0.05).


CONCLUSIONS
DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.
Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China. sxshwyq@sina.com
Hua-Wen
HW
Sun
Qi-Bing
QB
Tang
Yong-Jun
YJ
Cheng
Sheng-Qian
SQ
Zou
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D016219:Immunotherapy, Adoptive; D051379:Mice; D008807:Mice, Inbred BALB C; D013274:Stomach Neoplasms / Q000276:immunology* / Q000503:physiopathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014020:Tissue Extracts / Q000276:immunology; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D000954:Antigens, Surface; D014020:Tissue Extracts; D016159:Tumor Suppressor Protein p53