Membrane bistability in thalamic reticular neurons during spindle oscillations.
The thalamic reticular (RE) nucleus is a major source of inhibition in the thalamus. It plays a crucial role in regulating the excitability of thalamocortical networks and in generating some sleep rhythms. Current-clamp intracellular recordings of RE neurons in cats under barbiturate anesthesia revealed the presence of membrane bistability in approximately 20% of neurons. Bistability consisted of two alternate membrane potentials, separated by approximately 17-20 mV. While non-bistable (common) RE neurons fired rhythmic spike-bursts during spindles, bistable RE neurons fired tonically, with burst modulation, throughout spindle sequences. Bistability was strongly voltage dependent and only expressed under resting conditions (i.e. no current injection). The transition from the silent to the active state was a regenerative event that could be activated by brief depolarization, whereas brief hyperpolarizations could switch the membrane potential from the active to the silent state. These effects outlasted the current pulses. Corticothalamic stimulation could also switch the membrane potential from silent to active states. Addition of QX-314 in the recording micropipette either abolished or disrupted membrane bistability, suggesting I(Na(p)) to be responsible for its generation. Thalamocortical cells presented various patterns of spindling that reflected the membrane bistability in RE neurons. Finally, experimental data and computer simulations predicted a role for RE neurons' membrane bistability in inducing various patterns of spindling in target thalamocortical cells. We conclude that membrane bistability of RE neurons is an intrinsic property, likely generated by I(Na(p)) and modulated by cortical influences, as well as a factor that determines different patterns of spindle rhythms in thalamocortical neurons.
Laboratoire de Neurophysiologie, Faculté de Médecine, Université Laval, Québec, G1K 7P4, Canada.
Pablo
P
Fuentealba
Igor
I
Timofeev
Maxim
M
Bazhenov
Terrence J
TJ
Sejnowski
Mircea
M
Steriade
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D002415:Cats; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D004569:Electroencephalography / Q000379:methods; D005071:Evoked Potentials; D019706:Excitatory Postsynaptic Potentials; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology* / Q000502:physiology*; D008012:Lidocaine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000145:classification / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D010507:Periodicity; D013569:Synapses / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
D000779:Anesthetics, Local; C070614:neurobiotin; C012647:QX-314; D001710:Biotin; D008012:Lidocaine