Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila.
Among the four methods for extracting extracellular polymeric substances (EPS) from Rhodopseudomonas acidophila (EDTA, NaOH, H(2)SO(4), heating/centrifugation), EDTA extraction was found to be the most effective. The contents of the major components of EPS from R. acidophila, i.e., carbohydrate, protein and nucleic acid, were 6.5, 58.4 and 5.4 mg g(-1) dry cells, respectively. The optimum extraction time was 1-3 h and the EDTA dosage was approximately 2.8 g g(-1) dry cells. Under these conditions, no cell lysis was observed. The EPS content and the percentage of the three main components were greatly dependent on the extraction method. The intensity of absorption peaks for photosynthetic pigments in the UV-visible spectrum of bacteria remained unchanged prior to and after EDTA extraction; and no pigment peaks appeared in the EPS spectrum. This suggests that few cells were destroyed and lysis did not occur. UV-visible spectrum analysis, an easy and rapid technique, could be used to monitor cell lysis during EPS extraction from R. acidophila.
Department of Chemistry, Laboratory of Environmental Biotechnology, University of Science and Technology of China, Hefei, Anhui 230026, China.
Guo-Ping
GP
Sheng
Han-Qing
HQ
Yu
Zhou
Z
Yu
D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D001704:Biopolymers / Q000032:analysis* / Q000302:isolation & purification*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004492:Edetic Acid; D009696:Nucleic Acids / Q000032:analysis / Q000302:isolation & purification; D045322:Photosynthetic Reaction Center Complex Proteins / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000302:isolation & purification; D012241:Rhodopseudomonas / Q000737:chemistry*; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D001704:Biopolymers; D009696:Nucleic Acids; D045322:Photosynthetic Reaction Center Complex Proteins; D011135:Polysaccharides, Bacterial; D004492:Edetic Acid