Nutritional deficiency affects cell cycle status and viability in A549 cells: role of p27Kip1.
We investigated how nutritional deficiency affects cell cycle and cell viability in A549 lung adenocarcinoma cells. Deprivation of various amino acids or glucose induced cell cycle arrest and cell death in a different manner. Cell death on deprivation of these nutrients was increased by downregulating of p27Kip1 with RNA interference. It was also observed that intrinsic p27Kip1 was segregated in cytoplasm in a glucose-deprived situation. In conclusion, amino acid or glucose deprivation induced cell cycle arrest and cell death, part of which is thought to be rescued by the existence of cytoplasmic p27Kip1.
Department of Pulmonary Medicine, Yokohama City University Medical Center, 4-57, Urahune-cho, Minami-ku, Yokohama City 232-0024, Japan.
Takeo
T
Ishii
Mina
M
Fujishiro
Michiaki
M
Masuda
Koji
K
Okudela
Hitoshi
H
Kitamura
Shinji
S
Teramoto
Takeshi
T
Matsuse
D000230:Adenocarcinoma / Q000473:pathology*; D000596:Amino Acids / Q000378:metabolism; D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D002470:Cell Survival; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D015536:Down-Regulation; D016147:Genes, Tumor Suppressor; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D044342:Malnutrition; D034622:RNA Interference; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*
D000596:Amino Acids; D018797:Cell Cycle Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases; D005947:Glucose