Mitochondrial function in turkey skeletal muscle--impact on meat quality.
1. M. iliotibialis (MIT) and M. pectoralis (MP) of the BUT Big 6 and Kelly BBB turkey were characterised with respect to physical properties, mitochondrial function, metabolic state, morphology and meat quality. 2. Mitochondrial enzyme activity and respiration rates in MP declined with increasing age while glycolytic enzyme activity remained nearly constant. 3. There were no major differences between BUT Big 6 and Kelly BBB with respect to histological, histochemical or biochemical variables. In spite of the greater adult weight of BUT Big 6, body proportion was equal in both strains. 4. In agreement with the histochemical findings MIT showed higher oxidative capacities, while glycolytic enzyme activity was higher in MP. 5. Pyruvate was the best substrate for oxidative phosphorylation in MIT, but not in MP. Pyruvate dehydrogenase (PDH) activity was up to 15 times less in MP and blood lactate was correlated with intramuscular pH. 6. Turkey breast muscle was restricted in its ability to oxidise pyruvate, especially in those animals that tended to develop intramuscular acidosis post mortem. 7. It is concluded that the in vivo metabolic environment results in acidosis and impaired meat quality, at least in turkey M. pectoralis.
Muskellabor der Klinik und Poliklinik für Neurologie der Martin-Luther-Universität, Halle-Wittenberg, Halle (Saale), Germany.
J R
JR
Opalka
M
M
Wicke
F N
FN
Gellerich
R
R
Schmidt
F
F
Rosner
S
S
Zierz
G
G
von Lengerken
D000138:Acidosis; D000375:Aging; D000818:Animals; D001823:Body Composition; D006019:Glycolysis; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000097:blood; D008460:Meat; D008854:Microscopy, Electron; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000254:growth & development / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011180:Postmortem Changes; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D011786:Quality Control; D012519:Sarcoplasmic Reticulum / Q000502:physiology; D014422:Turkeys
D011768:Pyruvate Dehydrogenase Complex; D019344:Lactic Acid; D019289:Pyruvic Acid