Inhibitory mechanism of inteferon-gamma on human fibroblasts from Tenon's capsule.
The inhibitory mechanism of interferon-gamma (IFN-gamma) on the fibroblasts from Tenon's capsule was studied. By using immunohistochemical SP method and pathological image system, the inhibitory effects of IFN-gamma on the expression of transforming growth factor beta receptor I in the in vitro cultured fibroblasts from Tenon's capsule were quantitatively analyzed. The results showed that IFN-gamma could reduce the expression of transforming growth factor beta receptor I in the fibroblasts with the following dose-effect relationship: Y = 1937.5-134.2 Igx (r=-0.971, P<0.01). It was concluded that IFN-gamma could inhibit the expression of transforming growth factor beta receptor I in the fibroblasts from Tenon's capsule. The modulation of the transforming growth factor beta receptor I expression by IFN-gamma may be beneficial to the alleviation of the hyperplasia of scar after trabeculectomy.
Han
Bo
B
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 43002, China.
Hu
Yizhen
Y
Xiong
Xinchun
X
D003228:Conjunctiva / Q000378:metabolism / Q000473:pathology*; D003238:Connective Tissue / Q000187:drug effects*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D018463:Filtering Surgery; D005901:Glaucoma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis
D018125:Receptors, Transforming Growth Factor beta; D007371:Interferon-gamma