Inhibition of Streptococcus pyogenes adherence to HaCaT cells by a peptide corresponding to the streptococcal fibronectin-binding protein, SfbI, is strain dependent.
Streptococcus pyogenes (group A streptococcus, GAS) is a human-specific pathogen, which employs a large number of adhesins for colonization. Fibronectin-binding proteins (FBPs) play a major role in GAS adhesion to host cells. SfbI, a major streptococcal FBP, has been well studied. A peptide (peptide-MSG) based on this adhesin inhibits fibronectin (Fn)-binding by the pathogen. To test whether this peptide also inhibits adherence of GAS to host cells, adhesion assays were performed with strains possessing different combinations of genes for three distinct FBPs. Peptide-MSG inhibited GAS adherence to human keratinocytes (HaCaT) in a strain dependent manner. There is no consistent pattern between the effect and the ability to express one or more of the FBPs. A single peptide may be insufficient to prevent GAS adherence to host cells.
Menzies School of Health Research, Darwin, Australia.
Mandy L
ML
Edwards
Peter K
PK
Fagan
Rebecca J
RJ
Towers
Bart J
BJ
Currie
Kadaba S
KS
Sriprakash
D018829:Adhesins, Bacterial / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D013297:Streptococcus pyogenes / Q000187:drug effects* / Q000502:physiology
D018829:Adhesins, Bacterial; D005353:Fibronectins; D010446:Peptide Fragments; C078635:fibronectin-binding proteins, bacterial