Cytogenetic and molecular cytogenetic analyses in diffuse astrocytomas.
Diffuse astrocytomas are highly variable tumors and show complex biologic behavior that is based on multi-step oncogenesis. We report cytogenetic and molecular cytogenetic investigations in 23 cases of diffuse astrocytomas. The results of conventional karyotyping, interphase fluorescence in situ hybridization (FISH), comparative genomic hybridization, multicolor FISH, and spectral karyotyping are reported. Various numerical and structural chromosomal aberrations were identified. Clustering of structural alterations in the short arm of chromosome 2 (2p) and the long arm of chromosome 7 (7q) were detected. Using spectral karyotyping, additional chromosome rearrangements not detectable by conventional methods were found. Some of these anomalies have not been previously described in diffuse astrocytomas. An independent validation of these discrepant findings is required.
Department of Neurosurgery, University of Leipzig, Liebigstrasse 20, 04103 Leipzig, Germany. wkrupp@medizin.uni-leipzig.de
Wolfgang
W
Krupp
Kathrin
K
Geiger
Ralf
R
Schober
Gerlinde
G
Siegert
Ursula G
UG
Froster
D000782:Aneuploidy; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor / Q000648:ultrastructure; D002869:Chromosome Aberrations; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping / Q000379:methods; D009693:Nucleic Acid Hybridization; D032681:Spectral Karyotyping