Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.
Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan. f0512@cc.nagasaki-u.ac.jp
Maria Cecilia
MC
Huaman
Yoshiya
Y
Sato
Jose Luis
JL
Aguilar
Angelica
A
Terashima
Humberto
H
Guerra
Eduardo
E
Gotuzzo
Hiroji
H
Kanbara
D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods* / Q000592:standards; D000704:Analysis of Variance; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D015490:HTLV-I Infections / Q000150:complications; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis*
D000947:Antigens, Helminth