Effect of hemolysis on cardiac troponin T determination by the Elecsys 2010 immunoanalyzer.
OBJECTIVE
To evaluate the effect of hemolysis on the measurement of cardiac troponin T (cTnT) using the Elecsys 2010 immunoanalyzer.


METHODS
cTnT concentrations were measured using the Elecsys 2010. Interference studies were conducted by mixing serum of known cTnT concentration with either hemolysates or serum to which purified hemoglobin (Hgb) had been added. Hemolysates were prepared by mechanical disruption. PROBIT analysis was conducted to determine the probability of a cTnT result of >0.1 microg/l being decreased to <0.1 microg/l due to hemoglobin interference.


RESULTS
Purified hemoglobin as well as serum-hemolysates mediated a concentration-dependent inhibition of cTnT determination with the Elecsys 2010. With every 1 g/l increase in hemoglobin, the probability of cTnT >0.1 is decreased by 2.5%.


CONCLUSIONS
A concentration-dependent negative bias in the measurement of cTnT is associated with increasing hemoglobin concentrations in serum.
Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, AB, Canada. martha.lyon@cls.ab.ca
Martha E
ME
Lyon
Cindy L
CL
Ball
Richard D
RD
Krause
George A
GA
Slotsve
Andrew W
AW
Lyon
D002624:Chemistry, Clinical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis / Q000096:biosynthesis; D006461:Hemolysis; D006801:Humans; D015233:Models, Statistical; D009206:Myocardium / Q000378:metabolism*; D015203:Reproducibility of Results; D013053:Spectrophotometry; D020107:Troponin T / Q000032:analysis* / Q000096:biosynthesis
D006454:Hemoglobins; D020107:Troponin T; D004492:Edetic Acid