Assessment of oxidative stress in the spontaneously hypertensive rat brain using electron spin resonance (ESR) imaging and in vivo L-Band ESR.
This study examined the blood brain barrier (BBB)-permeable nitroxyl compound, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (MC-PROXYL), as a spin probe for the assessment of oxidative stress in the brain by electron spin resonance (ESR) imaging and in vivo L-band ESR. Preliminary comparisons were made by ESR imaging of MC-PROXYL in the isolated brains of normal Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and stroke prone SHR (SHRSP). The decay of the ESR images of MC-PROXYL in the isolated brains was faster in SHR than in normal WKY, but was only moderate in SHRSP. In addition, the decay rate of MC-PROXYL in the heads of live rats, as measured noninvasively by L-band ESR, was faster in SHR than in WKY, and was slower in SHR than in SHRSP. Taken together, our data suggest that the oxidative stress of SHR is not as high as that in high oxidative stress animal models such as SHRSP. This is the first study to present reconstructed 3D images of the distribution of MC-PROXYL in the isolated SHR brain. The ESR technique employed herein appears to be a powerful tool for evaluating oxidative stress and for detecting the region of oxidative stress in the brain of SHR.
Lee
Masaichi-Chang-Il
MC
Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College, Yokosuka, Japan.
Shoji
Hirofumi
H
Miyazaki
Hiroyuki
H
Yoshino
Fumihiko
F
Hori
Norio
N
Toyoda
Minoru
M
Ikeda
Yukio
Y
Anzai
Kazunori
K
Ikota
Nobuo
N
Ozawa
Toshihiko
T
D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D020022:Genetic Predisposition to Disease; D006973:Hypertension / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008297:Male; D018384:Oxidative Stress; D011759:Pyrrolidines / Q000493:pharmacokinetics; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics / Q000378:metabolism*; D011921:Rats, Inbred WKY; D013113:Spin Labels; D020521:Stroke / Q000235:genetics; D014018:Tissue Distribution
D003497:Cyclic N-Oxides; C109806:MCPROXYL; D011759:Pyrrolidines; D013113:Spin Labels