Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides.
A versatile microplate bioassay for quick and sensitive determination of antibacterial activity was developed for use in screening medicinal plants and identification of their active principles. This assay can be used to determine minimum inhibitory concentrations for small quantities of organic or water-soluble plant extracts. Bioassay-guided fractionation of the stem and leaves of Peperomia galioides using this method found fractions containing grifolin and grifolic acid, which inhibited growth of Staphylococcus aureus and Staphylococcus epidermidis.
Department of Chemistry and Biochemistry, University of Massachusetts-Dartmouth, North Dartmouth 02747, USA.
Richard D
RD
Langfield
Frank J
FJ
Scarano
Mary E
ME
Heitzman
Miwako
M
Kondo
Gerald B
GB
Hammond
Catherine C
CC
Neto
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D036941:Peperomia; D010568:Peru; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D018547:Plant Stems; D010946:Plants, Medicinal; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D010936:Plant Extracts