[The expression and significance of costimulatory molecule in peripheral blood B lymphocytes in rheumatoid arthritis].
OBJECTIVE
To research the mechanisms of immune function disorder in the patients with rheumatoid arthritis (RA).


METHODS
Monoclonal antibodies against CD(80), CD(86), CD(40) were used for flow cytometry and expression of costimulatory molecule CD(80), CD(86), CD(40) on peripheral blood B lymphocytes was studied in patients with RA, and healthy human were used as control groups. We also used enzyme linked immunosorbent assay to detect the cytokines level of Th1 cell including interleukin (IL)-2, interferon-gamma and those of Th2 cell including IL-6, IL-10. These samples are serum and synovial fluid of patients with RA, respectively.


RESULTS
Compared with control group, the expression of CD(86) on peripheral blood B lymphocytes in patients with RA was obviously decreased (P < 0.01), the expression of CD(40) was also significantly increased (P < 0.05). No statistically significant differences in CD(80) positive peripheral blood B lymphocytes between RA group and control group were observed. Comparing with control group, the level of IL-2 and interferon-gamma in serum and synovial fluid of patients with RA are obviously increase (P < 0.01 or P < 0.05). While, the level of IL-6 and IL-10 are decrease (P < 0.01 or P < 0.05).


CONCLUSIONS
The disorder expression of costimulatory molecule CD(86), CD(40) on peripheral blood B lymphocytes may be associated closely with the imbalance of Th1/Th2 cytokines level in patients with RA. These will give us a new idea on the therapeutic strategy of RA.
Central Laboratory, Hangzhou First People's Hospital, Hangzhou 310006, China. lengjh5@hotmail.com
Jian-hang
JH
Leng
Zhi-yong
ZY
Hu
Guang-chao
GC
Zhuo
Ke-yi
KY
Wang
Zi-wei
ZW
Wang
Liang
L
Peng
Yu-fang
YF
Zhang
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018122:B7-1 Antigen / Q000097:blood; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood; D008875:Middle Aged; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; D016207:Cytokines; D008562:Membrane Glycoproteins