Possible direct induction by estrogen of calcitonin secretion from ultimobranchial cells in the goldfish.
The plasma level of calcitonin (CT), a calcium (Ca)-regulating hormone, is known to increase in female teleosts during the reproductive period. In the present study, a correlation between plasma CT and Ca and one between plasma CT and the gonad somatic index were demonstrated in the female goldfish but not in the male. To clarify the relationship between CT and Ca, we examined the plasma CT and Ca levels after injecting immature goldfish with estrogen. At day 1, the plasma CT level significantly increased, whereas the plasma Ca level was not changed from its initial level. This result suggests that the trigger of CT secretion is estrogen and that estrogen directly acts on the ultimobranchial gland (UBG), a CT-secreting organ. To determine whether the UBG is equipped with estrogen receptor (ER), an ER binding assay and immunohistochemical staining of UBG cells with an antibody against ER were conducted. As a result, estrogen-specific binding (Kd, 18.52 nM; Bmax, 1.35 pmol/mg protein) and ER-immunoreactivity in the UBG were demonstrated. Furthermore, the expression of alpha, beta, and gamma types of ER in the UBG was also detected by use of the reverse-transcription polymerase chain reaction. Thus, we concluded that estrogen acts on the UBG to induce the release of CT, which in turn plays an important role in reproduction directly and/or indirectly through Ca. This is the first report on the existence of ERs in a teleost UBG and the occurrence of CT secretion caused by estrogen.
Suzuki
Nobuo
N
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Uchiura, Ishikawa 927-0553, Japan. nobuo@kenroku.kanazawa-u.ac.jp
Yamamoto
Kazutoshi
K
Sasayama
Yuichi
Y
Suzuki
Tohru
T
Kurokawa
Tadahide
T
Kambegawa
Akira
A
Srivastav
Ajai K
AK
Hayashi
Shinji
S
Kikuyama
Sakaé
S
D000818:Animals; D002116:Calcitonin / Q000097:blood / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D004958:Estradiol / Q000502:physiology*; D005260:Female; D006054:Goldfish / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009929:Organ Size; D010053:Ovary / Q000254:growth & development; D011897:Random Allocation; D011960:Receptors, Estrogen / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012737:Sex Factors; D012741:Sexual Maturation / Q000502:physiology; D014460:Ultimobranchial Body / Q000166:cytology / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol; D002116:Calcitonin; D002118:Calcium