CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins.
Cytotoxic T lymphocytes (CTL) are important for controlling equine infectious anemia virus (EIAV). Because Gag matrix (MA) and capsid (CA) are the most frequently recognized proteins, the hypothesis that CTL from EIAV-infected horses with diverse MHC class I alleles recognize epitope clusters (EC) in these proteins was tested. Four EC were identified by CTL from 15 horses and 8 of these horses had diverse MHC class I alleles. Two of the eight had CTL to EC1, six to EC2, five to EC3, and four to EC4. Because EC2-4 were recognized by CTL from >50% of horses with diverse alleles, the hypothesis was accepted. EC1 and EC3 were the most conserved EC and these more conserved broadly recognized EC may be most useful for CTL induction, helping overcome MHC class I polymorphism and antigenic variation.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99165-7040, USA.
Chungwon
C
Chung
Robert H
RH
Mealey
Travis C
TC
McGuire
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology*; D002353:Carrier State / Q000276:immunology* / Q000821:virology; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D004859:Equine Infectious Anemia / Q000276:immunology; D015683:Gene Products, gag / Q000737:chemistry / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000276:immunology*
D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag; D015395:Histocompatibility Antigens Class I; D014763:Viral Matrix Proteins