Somatostatin controls LFA-1 gene expression by altering neuraminidase expression in spleen cells.
This study investigated whether neuraminidase (Neu) affects LFA-1 mRNA expression in spleen cells and whether somatostatin (SOM) and substance P (SP) treatment induce changes in the Neu mRNA expression level in spleen cells. Neu treatments down-regulated the LFA-1 mRNA levels after culturing for 2 h. SOM increased the Neu mRNA level slightly after 24-h culture and strongly after 48-h culture. These results suggest that prolonged exposure to SOM may regulate the Neu activation pathway, which in turn impairs the regulation of LFA-1 expression.
Biopotency Evaluation Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Korea.
Won-Kee
WK
Yoon
Ho-Jun
HJ
Kim
Hwa-Young
HY
Son
Kyu-Shik
KS
Jeong
Sang-Joon
SJ
Park
Tae-Hwan
TH
Kim
Sung-Ho
SH
Kim
Se-Ra
SR
Kim
Si-Yun
SY
Ryu
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D009439:Neuraminidase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013004:Somatostatin / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology
D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D013373:Substance P; D013004:Somatostatin; D009439:Neuraminidase