Creation of a transgenic mouse for hair-cell gene targeting by using a modified bacterial artificial chromosome containing Prestin.
We made a transgenic mouse that expresses Cre recombinase activity in inner ear hair cells by using a modified bacterial artificial chromosome containing Prestin. Cre recombinase activity was restricted to inner and outer hair cells, a subset of vestibular hair cells, spiral and vestibular ganglia in the inner ear, and a subset of cells in the testis, epididymis, and ear bone. This mouse will be useful for hair-cell-specific gene targeting.
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee.
Yong
Y
Tian
Mingyuan
M
Li
Bernd
B
Fritzsch
Jian
J
Zuo
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004822:Epididymis / Q000378:metabolism; D018390:Gene Targeting; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism*; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*; D019426:Integrases / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020409:Molecular Motor Proteins; D011506:Proteins / Q000235:genetics*; D013737:Testis / Q000378:metabolism; D014725:Vestibular Nerve / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics*
D020409:Molecular Motor Proteins; C498720:Pres protein, mouse; D011506:Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases