Molecular characterisation of streptomycin-resistant Mycobacterium tuberculosis strains isolated in Poland.
Primary drug resistance of Mycobacterium tuberculosis strains in Poland increased two-fold between 1997 and 2000. Among 3705 drug-resistant strains investigated in 2000, 169 were resistant to streptomycin alone or in combination with isoniazid, rifampicin and/or ethambutol. The molecular basis of streptomycin resistance for 88 (52%) of these strains in comparison with 15 susceptible controls was determined. The most prevalent mutation was the single substitution Lys43Arg in the rpsL gene, found in 30.7% of the strains analysed. However, as many as 51% of the strains investigated carried no mutation in the rpsL or rrs genes. The multiple mutations present in two Beijing family strains were also identified.
Centre for Medical Biology, Polish Academy of Sciences, Lodz, Poland.
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A
Brzostek
A
A
Sajduda
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T
Sliwiński
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E
Augustynowicz-Kopeć
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A
Jaworski
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Z
Zwolska
J
J
Dziadek
D000904:Antibiotics, Antitubercular; D004252:DNA Mutational Analysis; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D013307:Streptomycin
D000904:Antibiotics, Antitubercular; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin