[Cyclic nucleotide regulation of intracellular Ca2+ release in secretory cells of salivary glands of Chironomus plumosus larvae].
We investigate action of cAMP and cGMP on calcium content in tissue of salivary gland of Chironomus plumosus larvae L. using arsenaso III. In order to permeabilize cells we incubate it in medium with saponin (0.1 mg/ml during 10 min) with next incubation in necessary solution. The internal incubation solution contents (in mM): 15.3 NaCl, 129.94 KCl, 0.35 Na2HPO4, 0.44 KH2PO4, 5.55 glucose, pH 7.0. It was shown that cAMP (1 mkM) didn't cause release calcium through IP3-channels, because IP3 didn't intensify affect of cAMP, which has been established before. We observed that cAMP (100 mkM) intensified ryanodine's action to increase calcium content in saponin-treated gland tissue. We concluded that cAMP (100 mkM) activated calcium releasing though ryanodine-sensitive channels and inhibited IP3-dependent calcium releasing without any stimulation. Different concentration of cGMP didn't cause change in calcium content in saponin-treated gland tissue. But at presence of tapsigargine in incubation medium cGMP (100 mkM) inhibited Ca2+-released channels.
S V
SV
Bychkova
V V
VV
Man'ko
M Iu
MIu
Klevets'
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002683:Chironomidae / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D007814:Larva / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology
D019837:Ryanodine Receptor Calcium Release Channel; D012503:Saponins; D019284:Thapsigargin; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium