Comparison of conventional and fast gas chromatography in human plasma fatty acid determination.
A fast gas chromatographic technique for the determination of fatty acids in human plasma was developed. Its validation and comparison to a conventional method are here reported. The fast method significantly reduced the time required for analysis by a factor of 5 (total time of 3.2 min) while maintaining a similar resolution. Reproducibility of qualitative and quantitative data was measured in both applications. The results demonstrated that the applied fast gas chromatography (GC) conditions do not affect the analytical quality of the assays, allowing a short analysis time.
Department de Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n 08028, Barcelona, Spain.
Isabel
I
Bondia-Pons
Ana I
AI
Castellote
M Carmen
MC
López-Sabater
D002849:Chromatography, Gas / Q000379:methods*; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
D005227:Fatty Acids