Cardiac function-related gene expression profiles in human atrial myocytes.
To obtain insights into the molecular pathogenesis of heart failure in humans, we have analyzed the expression profiles of>12,000 genes in a total of 17 human specimens of right atrial myocytes. From this large data set, we here tried to identify gene clusters, expression level of which is correlated precisely with clinical parameter values of cardiac function. We could reveal that cardiac myocytes with normal sinus rhythm were clearly differentiated, in the point of view of gene expression, from those with atrial fibrillation. Further, an expression profile-based prediction of arrhythmia by a newly developed "weighted-distance method" could efficiently diagnose our samples. We could even construct calculation formulae for the values of left ventricular ejection fraction based on the expression level of selected genes. To our best knowledge, this is the first report to indicate that pumping ability of heart can be predicted by any measures of atrium.
Division of Functional Genomics, Jichi Medical School, Kawachigun, Tochigi, Japan.
Ruri
R
Ohki-Kaneda
Jun
J
Ohashi
Keiji
K
Yamamoto
Shuichi
S
Ueno
Jun
J
Ota
Young Lim
YL
Choi
Koji
K
Koinuma
Yoshihiro
Y
Yamashita
Yoshio
Y
Misawa
Katsuo
K
Fuse
Uichi
U
Ikeda
Kazuyuki
K
Shimada
Hiroyuki
H
Mano
D000293:Adolescent; D000328:Adult; D000368:Aged; D001281:Atrial Fibrillation / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D005820:Genetic Testing / Q000379:methods; D006325:Heart Atria / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010641:Phenotype; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013318:Stroke Volume; D018487:Ventricular Dysfunction, Left / Q000150:complications / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism
D011506:Proteins