Candidate regions of tumor suppressor gene by loss of heterozygosity analysis on chromosome 8p11.1-q13.3 in gastric cancer.
Loss of heterozygosity (LOH) is an important event of tumorigenesis. In this paper, we report the comprehensive LOH analyses with microsatellite markers and their results at chromosome 8p11.1-q13.3 in gastric cancer. The microsatellite markers D8S2323 and D8S2330 exhibited high LOH frequencies, 54.2 and 57.1%, respectively. However, LOH at 8q showed no relationship to either histological types or stages of gastric cancer. Finally, we settled six candidate regions on 8q in gastric cancer where there was a high possibility of being the tumor suppressor gene(s), and concluded that the LOH of 8q occurred in the primary tumorigenesis of gastric cancer.
Kakinuma
Naoto
N
Novartis Pharma Tsukuba Research Institute, Ohkubo 8, Tsukuba-shi, Ibaraki 300-2611, Japan.
Kohu
Kazuyoshi
K
Sato
Masaaki
M
Yamada
Tatsuya
T
Nakajima
Motowo
M
Akiyama
Tetsu
T
Ohwada
Susumu
S
Shibanaka
Yasuhiko
Y
D002898:Chromosomes, Human, Pair 8; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*