Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain.
A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I alpha chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immuno-histochemistry using SLA class I alpha chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I alpha chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.
Tang
Wei-Ran
WR
Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
Kiyokawa
Nobutaka
N
Eguchi
Tomoko
T
Matsui
Jun
J
Takenouchi
Hisami
H
Honma
Daisuk
D
Yasue
Hiroshi
H
Enosawa
Shin
S
Mimori
Kenichi
K
Itagaki
Mitsuko
M
Taguchi
Tomoko
T
Katagiri
Yohko U
YU
Okita
Hajime
H
Amemiya
Hiroshi
H
Fujimoto
Junichiro
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification*; D019556:COS Cells; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007150:Immunohistochemistry; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen