Isolation and characterization of a serine/threonine protein kinase SOS2 gene from Brassica napus.
A full-length cDNA of a new serine/threonine (Ser/Thr) protein kinase gene, designated as BnSOS2 (GenBank Acc. No.AY310413), was cloned from Brassica napus by rapid amplification of cDNA ends (RACE). The full-length cDNA of BnSOS2 was 1779 bp and contained a 1539-bp open reading frame encoding a protein of 512 amino acids. Homology analysis shows that BnSOS2 strongly resembles other Ser/Thr protein kinase genes, and that its putative protein belongs to a typical Ser/Thr kinase family. Northern blot analysis reveals that BnSOS2 is salt-inducible. Our results indicate that BnSOS2 is a new member of the plant SOS2 gene family, which may play an important role in salt tolerance of plants.
Wang
Jin
J
School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology RD Center, Fudan University, Shanghai 200433, China.
Wu
Weisheng
W
Zuo
Kaijing
K
Fei
Jiong
J
Sun
Xiaofen
X
Lin
Juan
J
Li
Xuofeng
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018744:DNA, Plant; D017346:Protein Serine-Threonine Kinases