A polymeric immunoglobulin receptor-like milk protein with inhibitory activity on human immunodeficiency virus type 1 reverse transcriptase.
A protein with a molecular mass of 85 kDa and an N-terminal sequence resembling polymeric immunoglobulin receptor has been isolated from bovine milk. The isolation procedure involved removal of globulin from acid whey by precipitation with 1.8 M (NH(4))(2)SO(4) followed by addition of (NH(4))(2)SO(4) to attain a concentration of 3.6 M. Subsequent steps included chromatography on CM-Sepharose and Mono S and elution of the protein of interest with a linear NaCl concentration gradient. The polymeric immunoglobulin receptor-like milk protein inhibited HIV-1 reverse transcriptase (RT) with an IC(50) of 4.8 microM. However, it did not exhibit ribonuclease activity. Neither did it inhibit translation in a cell-free rabbit reticulocyte lysate system.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
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D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology; D002417:Cattle; D002845:Chromatography; D005260:Female; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D015497:HIV-1 / Q000201:enzymology*; D008894:Milk Proteins / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects*; D011817:Rabbits; D019056:Receptors, Polymeric Immunoglobulin / Q000235:genetics*; D012156:Reticulocytes / Q000201:enzymology
D019380:Anti-HIV Agents; D008894:Milk Proteins; D019056:Receptors, Polymeric Immunoglobulin; D054303:HIV Reverse Transcriptase