Reduced N-acetylaspartate in the temporal cortex of rats reared in isolation.
BACKGROUND
Isolation rearing of rats is a nonpharmacologic, nonlesion manipulation that leads to deficits in prepulse inhibition (PPI) and other behavioral and neurochemical alterations reminiscent of schizophrenia. N-acetylaspartate (NAA) is present in high concentrations in the central nervous system and is found primarily in neurons. N-acetylaspartate is considered to be a marker of both neuronal loss and cellular dysfunction. Magnetic resonance spectroscopy studies have shown reductions of cortical and hippocampal NAA in schizophrenia, and a recent postmortem study has demonstrated a regionally selective temporal cortex deficit.


METHODS
The aim of the present study was to determine whether rats reared in isolation exhibit deficits in PPI and reductions in NAA in discrete brain regions, namely the temporal cortex, frontal cortex, hippocampus, and striatum.


RESULTS
Compared with socially housed rats, isolation rearing resulted in PPI deficits (p <.05) and reductions in NAA in the temporal cortex (p <.001), with no significant change in the other regions investigated.


CONCLUSIONS
These results suggest a disturbance of neuronal function, reflected by NAA reductions in the temporal cortex in isolation-reared rats, providing further evidence that isolation rearing can mimic aspects of the neuronal pathology of schizophrenia.
Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom. M.K.Harte@Sheffield.ac.uk
Michael K
MK
Harte
Susan B
SB
Powell
Lindsay M
LM
Reynolds
Neal R
NR
Swerdlow
Mark A
MA
Geyer
Gavin P
GP
Reynolds
D000818:Animals; D000831:Animals, Newborn; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008297:Male; D009433:Neural Inhibition / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012934:Social Isolation; D013702:Temporal Lobe / Q000378:metabolism*
D001224:Aspartic Acid; C000179:N-acetylaspartate