Trypanocidal failure suggested by PCR results in cattle field samples.
The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after controlled treatment of 738 cattle from 10 villages in Kénédougou, Burkina Faso with isometamidium chloride and diminazene aceturate. Using three sets of primers, PCR was three-four times more sensitive and better at species identification, than standard microscopic examination. The better sensitivity and species specificity of PCR have important advantages for drug resistance studies in the field.
Institute for Parasitology and International Animal Health, Freie Universität Berlin, Koenigsweg 67, D-14163 Berlin, Germany.
Yvonne
Y
Gall
Tanja
T
Woitag
Burkhard
B
Bauer
Issa
I
Sidibe
John
J
McDermott
Dieter
D
Mehlitz
Peter-Henning
PH
Clausen
D000818:Animals; D002417:Cattle; D004133:Diminazene / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D004352:Drug Resistance, Microbial; D021261:Parasitic Sensitivity Tests / Q000379:methods*; D010617:Phenanthridines / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017211:Treatment Failure; D014344:Trypanocidal Agents / Q000627:therapeutic use*; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000188:drug therapy
D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride; C003915:diminazene aceturate; D004133:Diminazene