Decreased levels of myeloperoxidase in induced sputum of patients with COPD after treatment with oral glucocorticoids.
BACKGROUND
Inhaled glucocorticoids may decrease exacerbations in some patients with COPD, and oral glucocorticoids may improve FEV(1) and shorten hospital stay during exacerbations. The mechanism of these improvements is unknown. This study examines the effect of oral glucocorticoids on markers of neutrophilic airway inflammation.


METHODS
Eighteen patients with COPD received oral prednisone, 0.5 mg/kg/d for 2 weeks. Clinical status, lung function measurements, and sputum induction were performed before and after treatment with oral prednisone. Levels of the neutrophil chemoattractant (interleukin-8 [IL-8]) and neutrophil activation marker (myeloperoxidase [MPO]) were measured in the supernatant of induced sputum by enzyme-linked immunosorbent assay.


RESULTS
Levels of MPO decreased significantly after treatment with prednisone (p = 0.0004): before treatment median, 2.54 microg/mL (range, 1.49 to 12.58 microg/mL); after treatment median, 1.79 microg/mL (range, 1.32 to 3.57 microg/mL). Treatment with prednisone did not influence the levels of IL-8.


CONCLUSIONS
The treatment of patients with COPD with oral glucocorticoids decreases the activation of neutrophils, which may be partially responsible for clinical improvement in these patients.
Barczyk
Adam
A
Department of Pneumology, Silesian Medical Academy, Katowice, ul. Medykow 14, Poland. adagne@mp.pl
Sozañska
Ewa
E
Trzaska
Marzena
M
Pierzchala
Wladyslaw
W
D000284:Administration, Oral; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D018375:Neutrophil Activation / Q000187:drug effects; D009195:Peroxidase / Q000032:analysis*; D011241:Prednisone / Q000008:administration & dosage / Q000494:pharmacology; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy*; D013183:Sputum / Q000737:chemistry / Q000201:enzymology*
D005938:Glucocorticoids; D016209:Interleukin-8; D009195:Peroxidase; D011241:Prednisone