Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time RT-PCR.
We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. rgscerg@gsc.riken.jp
Harukazu
H
Suzuki
Rie
R
Okunishi
Wataru
W
Hashizume
Shintaro
S
Katayama
Noriko
N
Ninomiya
Naoki
N
Osato
Kenjiro
K
Sato
Mari
M
Nakamura
Juri
J
Iida
Mutsumi
M
Kanamori
Yoshihide
Y
Hayashizaki
D000375:Aging / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods*; D020407:Internet; D051379:Mice; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
D012333:RNA, Messenger; D014157:Transcription Factors