HRP/[Zn-Cr-ABTS] redox clay-based biosensor: design and optimization for cyanide detection.
A novel inexpensive and simple amperometric biosensor, based on the immobilization of HRP into redox active [Zn-Cr-ABTS] layered double hydroxide, is applied to the determination of cyanide. The electrochemical transduction step corresponds to the reduction at 0.0 V of ABTS+* enzymatically formed in the presence of H2O2. The biosensor has a fast response to H2O2 (8s) with a linear range of 1.7 x 10(-9) to 2.1 x 10(-6) M and a sensitivity of 875 mA M(-1) cm(-2). The apparent Michaelis-Menten constant (KMapp) is 12 microM. The detection of cyanide is performed via its non competitive inhibiting action on the HRP/[Zn-Cr-ABTS] electrode. The concentration range of the linear response and the apparent inhibition constant (ki) are 5 x 10(-9) to 4 x 10(-8) and 1.4 x 10 (-7) M, respectively.
Shan
Dan
D
Laboratoire d'Electrochimie Organique et de Photochimie Rédox, UMR CNRS 5630, Institut de Chimie Moléculaire de Grenoble, FR CNRS 2607, Université Joseph Fourier, Grenoble, France.
Cosnier
Serge
S
Mousty
Christine
C
D000538:Aluminum Silicates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D000077215:Clay; D003486:Cyanides / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013451:Sulfonic Acids / Q000138:chemical synthesis*
D000538:Aluminum Silicates; D003486:Cyanides; D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D009942:Organometallic Compounds; D013451:Sulfonic Acids; C500139:zinc(II) chromium(III) hydroxide 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonate; D006735:Horseradish Peroxidase; D000077215:Clay