Discrimination of human lung neoplasm from normal lung by two target genes.
Simple tools for discrimination of lung tissues can be useful in a fast machine-aided diagnosis, for example, by tumor-specific microarrays. We demonstrate that an easy ratio technique, based on the expression levels of only two genes differentially expressed in lung tumor and normal lung samples, allows discrimination of normal and neoplastic lung with a sensitivity of 100% and specificity of 90.5%. DNA microarray analysis of 99 lung tumor samples and 15 normal lung tissues revealed that receptor for advanced glycation end products (RAGE) mRNA is reduced fourfold (p = 7.8 x 10(-11)) and cyclin-B2 mRNA is upregulated twofold (p = 5.9 x 10(-18)) in lung carcinoma compared with normal lung. The microarray-calculated expression ratio of RAGE to cyclin-B2 was used in polymerase chain reaction analysis of 84 independent blinded samples to discriminate tumor and corresponding normal lung tissues. In 94.7% of the samples this quotient correctly distinguished non-small cell lung cancer from normal lung tissue, suggesting the RAGE/cyclin-B2 quotient as a potential means for diagnosis of lung cancer.
Department of Cardiothoracic Surgery, Martin Luther Universität Halle-Wittenberg, Ernst Grube Strasse 4, 06097 Halle, Germany. stefan.hofmann@medizin.uni-halle.de
Hans-Stefan
HS
Hofmann
Gesine
G
Hansen
Stefan
S
Burdach
Babett
B
Bartling
Rolf-Edgar
RE
Silber
Andreas
A
Simm
D000230:Adenocarcinoma / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D019926:Cyclin B / Q000235:genetics*; D056765:Cyclin B2; D005240:Feasibility Studies; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000556:secondary; D020411:Oligonucleotide Array Sequence Analysis; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics*; D015203:Reproducibility of Results
C503758:CCNB2 protein, human; D019926:Cyclin B; D056765:Cyclin B2; D005819:Genetic Markers; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic