[Screening urine markers of renal cell carcinoma using SELDI-TOF-MS].
OBJECTIVE
To screen relatively specifical markers in urines from renal cell carcinoma patients using surface-enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF-MS) ProteinChip technology.


METHODS
Urine samples from 40 renal cell carcinoma (RCC) patients, 40 healthy volunteers and 40 patients with other urogenital diseases were analyzed using IMAC-Cu-3 PoteinChip, which can specifically bind the metal-combining-proteins. Proteomic spectra were generated by mass spectrometry.


RESULTS
Four differentially expressed potentially biomarkers were identified with the relative molecular weights of 4020, 4637, 5070, and 5500. The sensitivity for diagnosing RCC was 57.5%, 66.7%, 63.7%, 65%, and specificity was 86.2%, 95%, 82.5% and 75% when the critical points were 2.0, 5.0, 5.0 and 5.0 respectively.


CONCLUSIONS
SELDI-TOF-MS ProteinChip technology is a quickly, easy and convenient, and high-throughput analyzing method capable of screening several relatively specific, potential biomarkers from the urines of RCC patients and had better clinical value.
Department of Urology, Shanghai 6th People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
Deng-Long
DL
Wu
Wen-Jing
WJ
Wang
Ming
M
Guan
San-Bao
SB
Jin
Chong-Rui
CR
Jin
Yuan-Fang
YF
Zhang
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000652:urine*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D014408:Biomarkers, Tumor