Birth of piglets through the non-surgical transfer of blastocysts produced in vitro.
In this study, we attempted to produce piglets by non-surgically transferring blastocysts produced in vitro, using a flexible catheter as the transfer instrument. Cumulus-oocyte complexes (COCs) were aspirated from the follicles of ovaries obtained at a local slaughterhouse. They were then matured in modified North Carolina State University (NCSU)-37 medium for 44-46 h and fertilized in porcine gamete medium (PGM). Ten hours after in vitro fertilization (IVF), presumptive zygotes were removed from the cumulus cells and cultured in porcine zygote medium (PZM)-5. Blastocysts were cultured for five days after IVF and, using a catheter for deep intrauterine insemination without sedation, they were transcervically transferred into the uterine horn of six recipients (45-50 blastocysts/recipients) whose estrous cycles were synchronized, at 5 days after human chorionic gonadotropin (hCG) injection. Of the six recipients, one sow became pregnant and farrowed seven piglets (four live piglets) 119 days after hCG injection. The body weight at birth of the newborns ranged from 0.8 to 1.4 kg. These results indicate that it is possible to obtain piglets by transcervically transferring blastocysts produced by IVF and in vitro cultures in chemically defined media.
Department of Production Diseases, National Institute of Animal Health, Tsukuba, Obaraki, Japan. schie@affrc.go.jp
Chie
C
Suzuki
Shokichi
S
Iwamura
Koji
K
Yoshioka
D000818:Animals; D001755:Blastocyst; D046149:Embryo Culture Techniques; D004624:Embryo Transfer / Q000295:instrumentation / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D008098:Litter Size; D011247:Pregnancy; D011256:Pregnancy Outcome; D034421:Sus scrofa