Protollin: a novel adjuvant for intranasal vaccines.
Protollin is a novel adjuvant comprising Proteosomes non-covalently complexed with LPS. Intranasal immunization of mice with Protollin combined with detergent-split influenza antigens (HA) or recombinant influenza hemagglutinin (rHA) enhanced serum IgG and mucosal IgA levels by up to 250-fold compared with immunization with the antigens alone. IFN-gamma responses were also enhanced compared to the levels produced by splenocytes from mice immunized with antigen alone, while production of IL-5 was abrogated. Mice immunized with Protollin-rHA were completely protected against lethal challenge with influenza virus, demonstrating that Protollin is an effective mucosal adjuvant for prophylactic vaccines.
ID Biomedical Corporation of Quebec, 7150 Frederick Banting, Ville St-Laurent, Montreal, QC, Canada H4S 2A1. tjones@idbiomedical.com
Taff
T
Jones
Sonya
S
Cyr
Francine
F
Allard
Nathalie
N
Bellerose
George H
GH
Lowell
David S
DS
Burt
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D002626:Chemistry, Pharmaceutical; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004337:Drug Carriers; D004338:Drug Combinations; D005260:Female; D006388:Hemagglutinins / Q000032:analysis / Q000276:immunology; D018928:Immunity, Mucosal / Q000187:drug effects*; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007252:Influenza Vaccines / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control; D013154:Spleen / Q000166:cytology; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D004337:Drug Carriers; D004338:Drug Combinations; D006388:Hemagglutinins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D008070:Lipopolysaccharides; D008081:Liposomes; C493785:Protollin; D014612:Vaccines; D014614:Vaccines, Synthetic; D003546:Cysteine Endopeptidases