Proteome study of human cerebrospinal fluid following traumatic brain injury indicates fibrin(ogen) degradation products as trauma-associated markers.
Traumatic brain injury (TBI), like other central nervous system pathologies, causes changes in the composition of cerebrospinal fluid (CSF). Consequently analysis of the CSF components is important to better understand the pathological processes involved in such diseases. The aim of this work was to identify specific markers of severe TBI. Proteomic analysis including two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to compare the CSF protein profile of severe TBI patients and controls. Proteins (alpha 1 antitrypsin, haptoglobin 1 alpha1, alpha2, and beta) belonging to the acute phase response showed an increased expression in severe TBI patients. Two other proteins, identified as proteolytic degradation products of the carboxyl-terminal portion of the fibrinogen beta, were present only in TBI patients. The presence of these markers could correlate with a post-traumatic local increase in fibrinolysis as well as to an inflammatory event following CNS tissue injury.
Proteomics Unit, San Raffaele Scientific Institute, Milan, Italy.
Antonio
A
Conti
Yovan
Y
Sanchez-Ruiz
Angela
A
Bachi
Luigi
L
Beretta
Elisabetta
E
Grandi
Massimiliano
M
Beltramo
Massimo
M
Alessio
D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D001930:Brain Injuries / Q000134:cerebrospinal fluid*; D002556:Cerebrospinal Fluid Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005340:Fibrinogen / Q000134:cerebrospinal fluid* / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D020543:Proteome
D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D020543:Proteome; D005340:Fibrinogen