Gene expression differences of regenerating rat liver in a short interval successive partial hepatectomy.
OBJECTIVE
To identify the genes expressed differentially in the regenerating rat liver in a short interval successive partial hepatectomy (SISPH), and to analyze their expression profiles.


METHODS
Five hundred and fifty-one elements selected from subtractive cDNA libraries were conformed to a cDNA microarray (cDNA chip). An extensive gene expression analysis following 0-36-72-96-144 h SISPH was performed by microarray.


RESULTS
Two hundred and sixteen elements were identified either up- or down-regulated more than 2-fold at one or more time points of SISPH. By cluster analysis and generalization analysis, 8 kinds of ramose gene expression clusters were generated in the SISPH. Of the 216 elements, 111 were up-regulated and 105 down-regulated. Except 99 unreported genes, 117 reported genes were categorized into 22 groups based on their biological functions. Comparison of the gene expression in SISPH with that after partial hepatectomy (PH) disclosed that 56 genes were specially altered in SISPH, and 160 genes were simultaneously up-regulated or down-regulated in SISPH and after PH, but in various amount and at different time points.


CONCLUSIONS
Genes expressed consistently are far less than that intermittently; the genes strikingly increased are much less than that increased only 2-5 fold; the expression trends of most genes in SISPH and in PH are similar, but the expression of 56 genes is specifically altered in SISPH. Microarray combined with suppressive subtractive hybridization can in a large scale effectively identify the genes related to liver regeneration.
Xu
Cun-Shuan
CS
College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan Province, China. xucs@x263.net
Zhang
An-Shi
AS
Han
Hong-Peng
HP
Yuan
Jin-Yun
JY
Chang
Cui-Fang
CF
Li
Wen-Qiang
WQ
Yang
Ke-Jin
KJ
Zhao
Li-Feng
LF
Li
Yu-Chang
YC
Zhang
Hui-Yong
HY
Rahman
Salman
S
Zhang
Jing-Bo
JB
D000818:Animals; D016000:Cluster Analysis; D005260:Female; D015870:Gene Expression; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000502:physiology*; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley