Enhancement of human immunodeficiency virus type 1 infectivity by replacing the region including Env derived from defective particles with an ability to form particle-mediated syncytia in CD4+T cells.
The infection and subsequent replication rates of human immunodeficiency virus type 1 (HIV-1) affect the pathogenicity. The initial stage of HIV-1 infection is largely regulated by viral envelope sequence. We previously reported that the defective doughnut-shaped particles produced from a persistently infected cell clone, named L-2, obtained from human CD4+ T-cell line MT-4 that was persistently infected with HIV-1 LAI strain, efficiently form particle-mediated syncytia with uninfected human CD4+ T-cell line, MOLT-4. Here, we prepared a molecular clone (pL2) containing the L-2 provirus to characterize the viral genetic region contributing to this activity to form particle-mediated syncytia. Several recombinants were constructed with pNL4-3 by replacing the pL2-derived region including full-length env. Characterization of the particles obtained by transfection with these recombinant clones confirmed that pL2-derived env carried the particle-mediated syncytia formation activity. It is noteworthy that the pL2-derived env region could also contribute to enhancement of infectivity in CD4+ T-cell lines as well as primary peripheral blood mononuclear cells (PBMCs). Thus, the HIV-1 particle-mediated syncytium formation activity could also contribute to the enhancement of HIV-1 infectivity.
Department of Virology, Research Institute for Microbial Disease, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Masanobu
M
Kinomoto
Tetsu
T
Mukai
Yong-Gang
YG
Li
Yukie
Y
Iwabu
Jiranan
J
Warachit
J Alejandro
JA
Palacios
Madiha S
MS
Ibrahim
Shoutaro
S
Tsuji
Toshiyuki
T
Goto
Kazuyoshi
K
Ikuta
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D015726:Giant Cells / Q000276:immunology / Q000648:ultrastructure / Q000821:virology; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D008854:Microscopy, Electron; D010957:Plasmids; D011533:Proviruses / Q000235:genetics; D011994:Recombinant Proteins; D014162:Transfection; D014771:Virion / Q000276:immunology / Q000648:ultrastructure; D014779:Virus Replication
D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins