Anticonvulsant activity and chemical composition of Artemisia dracunculus L. essential oil.
Artemisia dracunculus L. (Asteraceae) has been used orally as an antiepileptic remedy in Iranian folkloric medicine. The anticonvulsant potential and composition of the essential oil obtained from the aerial parts of the plant were assessed in this study. The essential oil exerted dose- and time-dependent antiseizure activity in both maximal electroshock (MES) and pentylenetetrazole models of experimental seizures with ED50 values of 0.84 and 0.26 ml/kg, respectively. At some anticonvulsant doses, the essential oil produced sedation and motor impairment assessed by rotarod test. Gas chromatography (GC)/mass spectrometry (MS) analysis of the essential oil revealed the presence of trans-anethole (21.1%), alpha-trans-ocimene (20.6%), limonene (12.4%), alpha-pinene (5.1%), allo ocimene (4.8%), methyl eugenol (2.2%), beta-pinene (0.8%), alpha-terpinolene (0.5%), bornyl acetate (0.5%) and bicyclogermacrene (0.5%) as the main components. The observed anticonvulsant and sedative effects could be related to the presence of monoterpenoids in the essential oil.
Sayyah
Mohammad
M
Department of Physiology and Pharmacology, Institute Pasteur of Iran, Tehran. sayyah@institute.pasteur.ac.ir
Nadjafnia
Leila
L
Kamalinejad
Mohammad
M
D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D019306:Artemisia; D004305:Dose-Response Relationship, Drug; D004597:Electroshock / Q000379:methods; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D010433:Pentylenetetrazole / Q000633:toxicity; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*
D000927:Anticonvulsants; D009822:Oils, Volatile; D010936:Plant Extracts; D010433:Pentylenetetrazole