Osmoadaptation-related genes in inner medulla of mouse kidney using microarray.
To distinguish biological molecular processes of osmotic stress occurring in inner medulla, we utilized microarrays to monitor expression profiles. RNAs from three segments (cortex, outer medulla, and inner medulla) of mouse kidney were isolated and applied to microarrays. We found 35 genes expressed highly in inner medulla. Next, microarrays for the RNAs from mouse medullary collecting duct cell line (mIMCD) cells and osmotically adapted mIMCD cells (HT cells) were performed (designed as resistant to 1270mOsm/H(2)O). Of 35 genes highly expressed in inner medulla, 6 genes such as; B-cell translocation gene protein (BTG), myc-basic motif homologue, gelsolin, cell surface glycoprotein, laminin beta2, and tubulo-interstitial nephritis antigen, were also expressed highly in HT cells. Using real-time PCR, we confirmed the expression of six genes. Additionally acute osmotic stress induced the BTG. By comparing the inner medulla to a mIMCD3, we identified genes which respond to acute and chronic hyperosmotic stress.
Yoshida
Takumi
T
Department of Blood Purification, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan. tyoshida@kc.twmu.ac.jp
Müller
Eva
E
Stears
Robin
R
Shirota
Satsuki
S
Tsuchiya
Ken
K
Akiba
Takashi
T
Gullans
Steven R
SR
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology*; D007668:Kidney / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism*; D007679:Kidney Medulla / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009997:Osmotic Pressure; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000502:physiology*
D014157:Transcription Factors