[Experimental study on p53 gene mutation in keloid fibroblasts].
OBJECTIVE
To explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts.


METHODS
Tissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts.


RESULTS
The points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples.


CONCLUSIONS
p53 gene mutation might play an important role in the formation and development of keloids.
Liu
Wang
W
Department of Plastic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P. R. China.
Jiang
You-Hui
YH
Li
You-Liang
YL
Lin
Zi-Hao
ZH
Jiang
Hua
H
Tan
Qian
Q
Zhang
Jian-Lin
JL
Wang
Zi-Min
ZM
D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D016158:Genes, p53; D006801:Humans; D007627:Keloid / Q000235:genetics*; D008297:Male; D009154:Mutation