Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.
The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes of the enzyme's catalytic machinery upon binding.
Gloster
Tracey M
TM
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, UK.
Williams
Spencer J
SJ
Roberts
Shirley
S
Tarling
Chris A
CA
Wicki
Jacqueline
J
Withers
Stephen G
SG
Davies
Gideon J
GJ
D017485:1-Deoxynojirimycin / Q000737:chemistry*; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004187:Disaccharides / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D050112:Imino Pyranoses; D010880:Piperidines / Q000737:chemistry*; D013054:Spectrophotometry, Atomic / Q000379:methods; D048170:Streptomyces lividans / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry*
D004187:Disaccharides; D004791:Enzyme Inhibitors; D050112:Imino Pyranoses; D010880:Piperidines; C098432:isofagomine; D017485:1-Deoxynojirimycin; D014995:Xylosidases; C004173:xylobiose