[Effect of genistein on expression of angiogenesis related factors in HER-2/neu-overexpressing breast cancer cells].
To study the molecular mechanism of inhibition of angiogenesis in HER-2/neu-overexpressing breast cancer by genistein, HER-2/neu negative expression breast cancer MCF-7 cells were transfected with HER-2/neu to establish HER-2/neu-overexpressing MCF-7 cells (named MCF-7/HER-2). Expression of vascular endothelial growth factor (VEGF), urokinase-type plasminogen activator (uPA), matrix metalloproteinase-2,9 (MMP-2,9) in MCF-7, MCF-7/HER-2 as well as genistein-treated MCF-7/HER-2 were measured by immunocytochemistry and Western blot. We found that the expression of VEGF, MMP-2,9 and uPA in MCF-7/HER-2 cells were highter than that in MCF-7 cells, those angiogenesis related factors expression in MCF-7/HER-2 cells significantly decreased after treatment with genistein. Genistein could inhibit expression of angiogenesis-related factors VEGF, MMP-2,9 and uPA in HER-2/neu-overexpressing breast cancer cells, and this may be part of molecular mechanism of its anti-angiogenesis in HER-2/neu-overexpressing breast cancer.
Department of Nutrition and Food Hygiene, Third Military Medical University, Chongqing 400038, China. yuxiaoping2005@sina.com
Xiao Ping
XP
Yu
Man Tian
MT
Mi
Jun Dong
JD
Zhu
D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D016588:Anticarcinogenic Agents; D019833:Genistein; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D018719:Erb-b2 Receptor Tyrosine Kinases; D014568:Urokinase-Type Plasminogen Activator; D042461:Vascular Endothelial Growth Factor A