[An improved animal model for chronic perforation of the tympanic membrane].
BACKGROUND
An inexpensive and valid animal model of chronic tympanic membrane perforation is needed.


METHODS
Twelve male rats were selected for different surgical procedures (subtotal tympanic membrane perforation with local microflaps, re-perforation without flaps, partial excision of the handle of malleus). The inhibition of spontaneous healing was accomplished by the application of prednisolon or mitomycin directly onto the tympanic membrane.


RESULTS
Only by additional partial excision of the handle of malleus followed by local application of mitomycin were we able to achieve a persistent tympanic membrane perforation.


CONCLUSIONS
Chronic tympanic membrane perforation using this procedure may be useful in further investigations of the medical impact of tympanic membrane healing.
Klinik und Poliklinik für Hals-Nasen-Ohren-Krankheiten, Kopf- und Halschirurgie der Ernst-Moritz-Arndt-Universität Greifswald.
H
H
Kaftan
W
W
Hosemann
A
A
Beule
D
D
Junghans
D000287:Administration, Topical; D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D008297:Male; D008866:Microsurgery / Q000379:methods; D016685:Mitomycin / Q000008:administration & dosage; D011239:Prednisolone / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D012008:Recurrence; D012086:Reoperation / Q000379:methods; D013524:Surgical Flaps; D014432:Tympanic Membrane / Q000473:pathology; D018058:Tympanic Membrane Perforation / Q000473:pathology / Q000601:surgery*; D014433:Tympanoplasty / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
D016685:Mitomycin; D011239:Prednisolone