[Therapeutic efficiency of amphotericin B liposome modified by RMP-7 to transport drug across blood brain barrier].
OBJECTIVE
To study the therapeutic efficiency of amphotericin B liposome (AmB-L) targeting to the brain in mice with meningitis.


METHODS
Amphotericin B liposome targeting to the brain were prepared by film-sonication method. Their concentration and encapsulation percentage were determined. The Candida albicans was injected into the brain of BALB/c mice and the meningitis model was set up. Then the therapeutic efficiency of amphotericin B liposome targeting to the brain was studied.


RESULTS
The encapsulation percentage of amphotericin B liposome was 93.3%. The meningitis model was set up after the Candida albicans was injected into the brain of BALB/c mice for 2 h. The therapeutic efficiency was increased after conjugating RMP-7 (the commercial nama is Cereport) to the surface of amphotericin B liposome.


CONCLUSIONS
The therapeutic efficiency of Amphotericin B liposome targeting to the brain in the mice with meningitis was better than that of the common amphotericin B liposome and the life of the mice in AmB-L-PEG-RMP-7 group was longer than that of the mice in AmB-L-PEG group and AmB-L-PEG + RMP-7 group.
Department of Physical Pharmacy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Xiao-bin
XB
Zhang
Shan
S
Yuan
Peng-cheng
PC
Lei
Xin-pu
XP
Hou
D000666:Amphotericin B / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002176:Candida albicans; D016503:Drug Delivery Systems; D005260:Female; D008081:Liposomes; D008297:Male; D016921:Meningitis, Fungal / Q000188:drug therapy* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley
D000935:Antifungal Agents; D008081:Liposomes; C068538:liposomal amphotericin B; C089876:RMP 7; D000666:Amphotericin B; D001920:Bradykinin