T2182C mutation in 23S rRNA is associated with clarithromycin resistance in Helicobacter pylori isolates obtained in Bangladesh.
Twelve clarithromycin-resistant (MIC, > or = 1 microg/ml) Helicobacter pylori isolates were analyzed for point mutations in the 23S rRNA gene. Sequence analysis of all of the resistant isolates revealed a T-to-C transition mutation at position 2182. Transformation experiments confirmed that a single T-to-C transition mutation at position 2182 is associated with clarithromycin resistance.
Laboratory Sciences Division, ICDDR, B, GPO Box 128, Dhaka 1000, Bangladesh.
Rasel
R
Khan
Shamsun
S
Nahar
Jinath
J
Sultana
Mian Mashhud
MM
Ahmad
Motiur
M
Rahman
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001459:Bangladesh / Q000453:epidemiology; D001483:Base Sequence; D017291:Clarithromycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017354:Point Mutation; D012329:RNA, Bacterial / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin