Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene.
OBJECTIVE
To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.


METHODS
BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.


RESULTS
DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.


CONCLUSIONS
BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.
Bai
Yang
Y
PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China. Baiyang1030@hotmail.com
Zhang
Ya-Li
YL
Chen
Ye
Y
Jin
Jian-Feng
JF
Zhang
Zhao-Shan
ZS
Zhou
Dian-Yuan
DY
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D016481:Helicobacter Infections / Q000517:prevention & control* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping
D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; C400429:BabA protein, Helicobacter pylori; D001428:Bacterial Vaccines; D011994:Recombinant Proteins