Morphological, histochemical, and immunocytochemical study of CO2 and Er:YAG laser effect on oral soft tissues.
OBJECTIVE
The purpose of this study was to investigate the morphological, histochemical, and immunocytochemical changes of the oral mucosa after CO(2) or Er:YAG laser irradiation.


BACKGROUND
There have been no comparative reports on CO(2) and Er:YAG laser effects on human oral soft tissues.


METHODS
Tissue preservation was studied in 40 oral biopsies of young patients obtained by CO(2) and Er:YAG laser surgery. Hematoxylin-eosin and Giemsa stains, PAS/diastase treatment, AE1 and AE2 cytokeratins, MiB1/Ki67, and bcl-2 immunoreactions were performed on the laser cut edges on formalin fixed, paraffin embedded biopsies.


RESULTS
CO(2) laser biopsies show blisters, clefts and erosions of the epithelium. Intracellular edema and lengthened nuclei were also seen. The glycogen content results decreased in CO(2) laser biopsies. Good expression for cytokeratins and cell-cycle proliferation markers were found in Er:YAG biopsies, on the contrary the apoptosis marker was better expressed in CO(2) laser biopsies.


CONCLUSIONS
The results suggest that Er:YAG laser may be routinely used in surgery, because of its minimal damage of the epithelial tissue, its low inflammatory reaction, its quicker healing process and its lower risk of scarring.
Zaffe
Davide
D
Department of Anatomy and Histology, University of Modena and Reggio Emilia, Italy.
Vitale
Marina C
MC
Martignone
Alessandra
A
Scarpelli
Francesco
F
Botticelli
Annibale R
AR
D000293:Adolescent; D001706:Biopsy; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D007742:Labial Frenum / Q000473:pathology / Q000601:surgery*; D053685:Laser Therapy / Q000295:instrumentation*; D009061:Mouth Mucosa / Q000473:pathology*; D014945:Wound Healing / Q000528:radiation effects*