Dynamic properties of nuclear pore complex proteins in gp210 deficient cells.
Gp210, an integral membrane protein of the nuclear pore complex (NPC), is believed to be involved in NPC biogenesis. To test this hypothesis, we have investigated dynamic properties of the NPC and distribution of NPC proteins in NIH/3T3 cells lacking gp210. POM121 (the other integral NPC protein) and NUP107 (of the NUP107/160 complex) were correctly distributed at the nuclear pores in the absence of gp210. Furthermore, fluorescence recovery after photobleaching experiments showed that POM121 and NUP107 remained stably associated at the NPCs. We conclude that gp210 cannot be required for incorporation of POM121 or NUP107 or be required for maintaining NPC stability.
Center for Biotechnology, Karolinska Institute, Huddinge, Sweden.
Charlotta
C
Eriksson
Cecilia
C
Rustum
Einar
E
Hallberg
D016475:3T3 Cells; D000818:Animals; D045744:Cell Line, Tumor; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000235:genetics*; D051379:Mice; D018613:Microscopy, Confocal; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000172:deficiency / Q000235:genetics*; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics*; D014162:Transfection
D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; C512902:Nup210 protein, mouse; D049452:Green Fluorescent Proteins