Catalase from the white shrimp Penaeus (Litopenaeus) vannamei: molecular cloning and protein detection.
Catalase is an antioxidant enzyme that plays a very important role in the protection against oxidative damage by breaking down hydrogen peroxide. It is a very highly conserved enzyme that has been identified from numerous species including bacteria, fungi, plants and animals, but the information about catalase in crustaceans is very limited. A cDNA containing the complete coding sequence for catalase from the shrimp Penaeus (Litopenaeus) vannamei was sequenced and the mRNA was detected by RT-PCR in selected tissues. Catalase was detected in hepatopancreas crude extracts by Western blot analysis with anti-human catalase polyclonal antibodies. The nucleotide sequence is 1692 bp long, including a 72-bp 5'-UTR, a coding sequence of 1515 bp and a 104-bp 3'-UTR. The deduced amino acid sequence corresponds to 505 amino acids with high identity to invertebrate, vertebrate and even bacterial catalases and contains the catalytic residues His71, Asn144, and Tyr354. The predicted protein has a calculated molecular mass of 57 kDa; which coincides with the size of the subunit (approximately 55 kDa) and the tetrameric protein (approximately 230 kDa) detected in hepatopancreas extracts under native conditions. Catalase mRNA level was higher in hepatopancreas, followed by gills and was not detected in muscle.
Tavares-Sánchez
Olga L
OL
Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, P.O. Box 1735, Hermosillo, Son 83000, Mexico.
Gómez-Anduro
Gracia A
GA
Felipe-Ortega
Ximena
X
Islas-Osuna
Maria A
MA
Sotelo-Mundo
Rogerio R
RR
Barillas-Mury
Carolina
C
Yepiz-Plascencia
Gloria
G
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001483:Base Sequence; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D043143:Hepatopancreas / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D033561:Penaeidae; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000975:Antioxidants; D018076:DNA, Complementary; D012333:RNA, Messenger; D002374:Catalase