In vitro expression of full-length and truncated bovine respiratory syncytial virus G proteins and their antibody responses in BALB/c mice.
Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, a significant and equivalent anti-BRSV G IgG response was elicited to the full-length and truncated BRSV G proteins. These constructs may be used to study host pathological and immunological responses.
Brady
Ryan P
RP
Department of Veterinary and Biomedical Sciences, University of Nebraska, East Campus Loop and Fair Street, Veterinary Basic Science, Lincoln, NE 68583-0905, USA.
Topliff
Christina L
CL
Kelling
Clayton L
CL
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D019556:COS Cells; D002417:Cattle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology* / Q000378:metabolism*; D014162:Transfection; D022223:Vaccines, Subunit / Q000096:biosynthesis / Q000276:immunology; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D004247:DNA; D019204:GTP-Binding Proteins