Candidate regions of tumor suppressor gene by loss of heterozygosity analysis on chromosome 8p11.1-q13.3 in gastric cancer.
Loss of heterozygosity (LOH) is an important event of tumorigenesis. In this paper, we report the comprehensive LOH analyses with microsatellite markers and their results at chromosome 8p11.1-q13.3 in gastric cancer. The microsatellite markers D8S2323 and D8S2330 exhibited high LOH frequencies, 54.2 and 57.1%, respectively. However, LOH at 8q showed no relationship to either histological types or stages of gastric cancer. Finally, we settled six candidate regions on 8q in gastric cancer where there was a high possibility of being the tumor suppressor gene(s), and concluded that the LOH of 8q occurred in the primary tumorigenesis of gastric cancer.
Novartis Pharma Tsukuba Research Institute, Ohkubo 8, Tsukuba-shi, Ibaraki 300-2611, Japan.
Naoto
N
Kakinuma
Kazuyoshi
K
Kohu
Masaaki
M
Sato
Tatsuya
T
Yamada
Motowo
M
Nakajima
Tetsu
T
Akiyama
Susumu
S
Ohwada
Yasuhiko
Y
Shibanaka
D002898:Chromosomes, Human, Pair 8; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*