URB expression in human bone marrow stromal cells and during mouse development.
Seven genes preferentially expressed in undifferentiated human bone marrow stromal cells (BMSC) with respect to BMSC-derived osteoblasts were previously identified by differential display. Here we characterize the expression of one of these genes, URB, belonging to the sushi-repeat-containing protein superfamily. In culture, URB is expressed in both human primary and cloned BMSC, and is drastically downregulated during osteoblastic differentiation of these cells. Here we show that in mouse tissues a single 3.8kb Urb transcript is detected and that the mouse Urb protein is secreted as a 150kDa glycoprotein. During mouse development Urb RNA is barely detectable in 9dpc embryos and increases at later stages. Both in situ hybridization and immunohistochemistry analysis show Urb expression in mouse embryos starting from 14dpc mostly in cartilage. The temporal and spatial expression pattern of Urb suggests its role in mouse skeletogenesis.
Liu
Yi
Y
Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, 16132 Genova, Italy. yiliu@cba.unige.it
Monticone
Massimiliano
M
Tonachini
Laura
L
Mastrogiacomo
Maddalena
M
Marigo
Valeria
V
Cancedda
Ranieri
R
Castagnola
Patrizio
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000378:metabolism*; D018076:DNA, Complementary; D015536:Down-Regulation; D004622:Embryo, Mammalian / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010012:Osteogenesis / Q000502:physiology*; D020539:Sequence Analysis, Protein; D017154:Stromal Cells / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
C492631:CCDC80 protein, human; C448780:Ccdc80 protein, mouse; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D025521:Tumor Suppressor Proteins