Crystallization and preliminary X-ray analysis of plant class I chitinase from rice.
We report here on crystallization and preliminary X-ray analysis of plant class I chitinase from rice (OsChia1b). Similar single crystals of full-length OsChia1b were obtained under two independent conditions. The crystals grown under these conditions diffracted up to 2.1 and 2.5 angstroms resolution, respectively, at a synchrotron beamline, and were found to belong to the tetragonal space group P4(3)2(1)2.
Kezuka
Yuichiro
Y
Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan.
Kitazaki
Keiji
K
Itoh
Yoshikane
Y
Watanabe
Jun
J
Takaha
Osamu
O
Watanabe
Takeshi
T
Nishizawa
Yoko
Y
Nonaka
Takamasa
T
D002688:Chitinases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins
D010940:Plant Proteins; D002688:Chitinases; C119825:chitinase C