[Matrix metalloproteinase-9 expression by endothelial cells incubated with peripheral blood leukocytes from patients with cerebral infarction].
OBJECTIVE
Matrix metalloproteinase-9 (MMP-9) have been implicatedin the development of cerebral infarction. In a prospective study we investigated whether the MMP-9 expression are increased by endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions.


METHODS
Using Immuno-fluorescence technique, Human umbilical vein endothelial cells were cocultured with leucocytes for 24 hours in vitro before used for detecting the MMP-9 expression. The leucocytes were purified from peripheral blood in 6 patients with acute cerebral infarction and in 6 age- and sex-matched healthy subjects. the smears of endothelial cells were stained with MMP-9 monoclonal antibodies, and detected using the ABC ELITE kit.


RESULTS
Laser-Confocus confirmed the presence of MMP-9 within endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions. Compared with the control group, MMP-9 expressions were significantly higher in the stroke group (P < 0.001).


CONCLUSIONS
Our study demonstrated that the leukocytes from acute cerebral infarction patients were Activated, and could stimulate vessel endothelial cells excrete MMP-9. Activated leukocytes and MMP-9 expression by endothelial cells may have an important role in acute cerebrovascular ischemia and its consequences.
Department of Neurology, 1st Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Ji-ying
JY
Zhou
Wei-wei
WW
Dong
D000368:Aged; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000097:blood / Q000201:enzymology*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000201:enzymology*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D011446:Prospective Studies; D014471:Umbilical Veins / Q000166:cytology / Q000201:enzymology
D015415:Biomarkers; D020780:Matrix Metalloproteinase 9