Optimization of L-lactic acid production from glucose by Rhizopus oryzae ATCC 52311.
The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration in a bubble column was conducted under optimal conditions. Results showed a maximum lactic-acid production rate of 2.58 g/L/h was obtained with an initial glucose concentration of 94 g/L. Final lactic-acid concentration of 83 g/L was achieved after 32 h of fermentation with a weight of 0.88 g lactic acid/g glucose consumed.
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA.
Y
Y
Zhou
J M
JM
Domínguez
N
N
Cao
J
J
Du
G T
GT
Tsao