Translation efficiency mediated by the 5' untranslated region greatly affects protein production in Aspergillus oryzae.
We demonstrate that the 5' untranslated region (5'UTR) plays an important role in determining translation efficiency in Aspergillus oryzae, using a model beta-glucuronidase (GUS) expression system. Alterations in the 5' UTR resulted in an increase in GUS activity of up to eight-fold, without affecting mRNA levels. Moreover, using the most effective 5'UTR construct, we could achieve remarkable intracellular overproduction of GUS protein; and the GUS level reached more than 50% of the total soluble protein. This is the first experimental evidence indicating the feasibility of improving recombinant protein yield by promoting translation initiation in filamentous fungi.
Koda
Akio
A
General Research Laboratory, Ozeki Corporation, 4-9, Imazu, Dezaike-cho, Nishinomiya-shi, Hyogo, 663-8227, Japan. akio.kouda@ozeki.co.jp
Minetoki
Toshitaka
T
Ozeki
Kenji
K
Hirotsune
Masato
M
D020121:5' Untranslated Regions / Q000235:genetics*; D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005656:Fungal Proteins / Q000096:biosynthesis*; D005966:Glucuronidase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis
D020121:5' Untranslated Regions; D005656:Fungal Proteins; D005966:Glucuronidase