[Effect of autologous bone marrow mesenchymal stem cells and extrogenous sodium hyaluronate on repairing knee joint defect in rabbits].
OBJECTIVE
To explore the relationship of the limited resource of the autologous bone marrow mesenchymal stem cells (MSCs) in articular cavity to the treatment results of full-thickness articular cartilage defect, and to investigate whether the extrogenous sodium hyaluronate (SH) promotes the migration of MSCs cultured in vitro to the articular defect in vivo.


METHODS
Sixty-six Japan rabbits were made the model of the full-thickness articular cartilage defect (5 mm width and 4 mm depth). The autologous MSCs were extracted from the rabbit femur, cultured in vitro, labeled by Brdu, and injected into the injured articular cavity with or without SH. The experiment was divided into 4 groups; group A (MSCs and SH, n=15); group B (MSCs, n=15); group C (SH, n=18); and group D (non-treatment, n=18). The morphologic observation was made by HE staining, Mallory staining and immunohistochemical staining after 5 weeks, 8 weeks and 12 weeks of operation.


RESULTS
There were significant differences in the thickness of repairing tissue between group A and group B (P<0.01); but there were no significant differences between group A and group C, and between group B and group D (P>0.05). The histological observation showed that the main repairing tissue was fibrocartilage in group A and fiber tissue in group B.


CONCLUSIONS
MSCs cultured in vitro and injected into the articular cavity can not improve the treatment results of the articular cartilage defect. Extrogenous SH has effect on repairing cartilage defect. The extrogenous SH has no effect on the chemotaxis of the MSCs, and on the collection of MSCs into the joint defect.
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China. orthop@public.cd.sc.cn
Qingquan
Q
Kong
Zhou
Z
Xiang
Siping
S
Xian
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002358:Cartilage, Articular / Q000293:injuries* / Q000601:surgery; D002478:Cells, Cultured; D005260:Female; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D007718:Knee Injuries / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D011817:Rabbits
D006820:Hyaluronic Acid