Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat.
Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive substances but also allergenic proteins that cause hypersensitive reactions. Thus, the development of hypoallergenic buckwheat would make this important pseudo-cereal available to allergic people. A major allergenic protein of buckwheat is Fag e 1. We isolated the respective cDNA, coding for a 22 kDa protein, from a recently developed autogamous strain of common buckwheat and confirmed its immunoglobulin E (IgE)-binding activity using recombinant Fag e 1 and sera of allergic patients. The derived amino acid sequence from Fag e 1 cDNA was used to synthesize an overlapping peptide library on nitrocellulose membranes for the determination of the Fag e 1 epitopes. We identified eight epitopes and the critical amino acids for IgE-binding within the epitopes. This epitope analysis of a major allergenic protein of buckwheat should help therapeutic efforts and aid in the development of hypoallergenic buckwheat.
Yoshioka
Hiroyuki
H
Graduate School of Agricultural and Biological Sciences, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka, Japan.
Ohmoto
Tsuyoshi
T
Urisu
Atsuo
A
Mine
Yoshinori
Y
Adachi
Taiji
T
D000485:Allergens / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D000939:Epitopes / Q000235:genetics* / Q000378:metabolism; D019612:Fagopyrum / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000276:immunology; D008222:Lymphokines / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D000485:Allergens; D052179:Antigens, Plant; D018076:DNA, Complementary; D000939:Epitopes; C492862:Fag e 1 allergen, Fagopyrum esculentum; D008222:Lymphokines; D010940:Plant Proteins; D011994:Recombinant Proteins; C013059:immunoglobulin-binding factors