Taurine protected myocardial mitochondria injury induced by hyperhomocysteinemia in rats.
Taurine can protect against cardiovascular diseases, whereas elevated levels of plasma homocysteine are associated with atherosclerotic and thromboembolic cardiovascular diseases. To illustrate the effects of taurine on hyperhomocysteinemia, we observed the myocardial mitochondria dysfunction in the rats with hyperhomocysteinemia induced by diet methionine loading, and the therapeutic effect of taurine. A methionine diet increased plasma homocysteine concentration (133.51 +/- 27.91 micromol/L vs 12.31 +/- 2.58 micromol/L in control, P < 0.01), stimulated the production of reactive oxygen species (ROS) in the myocardial mitochondria, and inhibited the activities of mitochondrial Mn-superoxide dismutase and catalase. The 45Ca uptake and Ca2+-ATPase activity in the myocardial mitochondria were significantly lowered in rats with hyperhomocysteinemia. Taurine supplements effectively attenuated the hyperhomocysteinemia-induced ROS production and inhibition of Mn-superoxide dismutase and catalase activities in the myocardial mitochondria, and increased its 45Ca uptake and Ca2+-ATPase activity. Thus, taurine antagonizes the oxidative stress injury in the myocardial mitochondria induced by the hyperhomocysteinemia.
Institute of Cardiovascular Diseases Research, First Hospital, Peking University, Beijing, P.R. China. changlin73@163.com
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D000818:Animals; D000838:Anions; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000517:prevention & control; D002374:Catalase / Q000378:metabolism; D006710:Homocysteine / Q000097:blood*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D008345:Manganese / Q000378:metabolism; D008715:Methionine / Q000737:chemistry / Q000494:pharmacology; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects / Q000473:pathology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides; D013654:Taurine / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D000838:Anions; D017382:Reactive Oxygen Species; D006710:Homocysteine; D013481:Superoxides; D013654:Taurine; D008345:Manganese; D008315:Malondialdehyde; D008715:Methionine; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase; D000252:Calcium-Transporting ATPases; D010100:Oxygen; D002118:Calcium