Determination of residual clenbuterol in pork meat and liver by HPLC with electrochemical detection.
OBJECTIVE
To detect the residual clenbuterol in pork meat and liver using HPLC with Coulometric electrode array system.


METHODS
Homogenized meat or liver sample was treated with 1 mol x L(-1) hydrochloric acid and centrifuged, the fat existing in meat or liver tissue was removed by diethyl ether. The pH of the remaining aqueous layer was adjusted to 10.8 +/- 0.2 or 11.6 +/- 0.2 for meat or liver and liquid-liquid extraction with diethyl ether was followed. The ether extract was evaporated to dryness, the residue was dissolved in the mobile phase. The mobile phase A consisted of 50 mmol x L(-1) phosphoric acid-30 mmol x L(-1) triethylamine and was adjusted to pH 4.0 with 2 mol x L(-1) sodium hydroxide solution. The mobile phase B consisted of methanol-acetonitrile (30:45). A mixture of mobile phase A and B (80:20) was used in the method. A four electrode array module was selected for quantitation, the electrode potentials were set at 450, 600, 650 and 680 mV respectively.


RESULTS
The two calibration curves for meat and liver showed good linearity between 1.88 - 60.16 ng x g(-1), the detection limit of clenbuterol was 1.2 ng x g(-1).


CONCLUSIONS
This method using HPLC-electrochemical detection is reproducible, and the sensitivity is good enough for the determination of clenbuterol in meat and liver.
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Xue-zhu
XZ
Zhang
Yi-ru
YR
Gan
Fu-nian
FN
Zhao
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002976:Clenbuterol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D013552:Swine
D002976:Clenbuterol