Alpha-methylacyl-CoA racemase: a multi-institutional study of a new prostate cancer marker.
OBJECTIVE
To test whether alpha-methylacyl-CoA racemase (AMACR) is a sensitive and specific marker of prostate cancer.


RESULTS
The expression levels of AMACR mRNA were measured by real-time polymerase chain reaction. A total of 807 prostatic specimens were further examined by immunohistochemistry specific for AMACR. Quantitative immunostaining analyses were carried out by using the ChromaVision Automated Cellular Imaging System and the Ariol SL-50 Imaging System, respectively. AMACR mRNA levels measured in prostatic adenocarcinoma were 55 times higher than those in benign prostate tissue. Of 454 cases of prostatic adenocarcinoma, 441 were positive for AMACR, while 254 of 277 cases of benign prostate were negative for AMACR. The sensitivity and specificity of AMACR immunodetection of prostatic adenocarcinomas were 97% and 92%, respectively. Both positive and negative predictive values were 95%. By automatic imaging analyses, the AMACR immunostaining intensity and percentage in prostatic adenocarcinomas were also significantly higher than those in benign prostatic tissue (105.9 versus 16.1 for intensity, 45.7% versus 0.02% and 35.03% versus 4.64% for percentage, respectively).


CONCLUSIONS
We have demonstrated the promising features of AMACR as a biomarker for prostate cancer in this large series and the potential to develop automated quantitative diagnostic tests.
University of Massachusetts medical School, Worcester, MA, USA.
Z
Z
Jiang
C L
CL
Wu
B A
BA
Woda
K A
KA
Iczkowski
P G
PG
Chu
M S
MS
Tretiakova
R H
RH
Young
L M
LM
Weiss
R D
RD
Blute
C B
CB
Brendler
T
T
Krausz
J C
JC
Xu
K L
KL
Rock
M B
MB
Amin
X J
XJ
Yang
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019998:Racemases and Epimerases / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase