Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor.
The Ca(2+)-binding protein calmodulin mediates cellular Ca(2+) signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions -858 and -728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between a GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.
Division of Applied Life Science (BK21 Program), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Hyeong Cheol
HC
Park
Man Lyang
ML
Kim
Yun Hwan
YH
Kang
Joo Mi
JM
Jeon
Jae Hyuk
JH
Yoo
Min Chul
MC
Kim
Chan Young
CY
Park
Jae Cheol
JC
Jeong
Byeong Cheol
BC
Moon
Ju Huck
JH
Lee
Hae Won
HW
Yoon
Sung-Ho
SH
Lee
Woo Sik
WS
Chung
Chae Oh
CO
Lim
Sang Yeol
SY
Lee
Jong Chan
JC
Hong
Moo Je
MJ
Cho
D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D017931:DNA Primers; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors