Inhibitory effect of topical applications of nondenatured soymilk on the formation and growth of UVB-induced skin tumors.
Treatment of female SKH-1 hairless mice with ultraviolet B light twice a week for 20 weeks resulted in a population of tumor-free mice with a high risk of developing skin tumors during the next several months in the absence of additional UVB treatment (high-risk mice). Topical applications of nondenatured soymilk but not heat-denatured soymilk once a day, 5 days a week to these high-risk mice inhibited the formation and growth of skin tumors. Similar topical applications of soybean trypsin inhibitor or Bowman-Birk inhibitor also inhibited the formation and growth of skin tumors, but these agents were less active than nondenatured soymilk. Treatment of miniswine skin with nondenatured soymilk once a day for 5 days prior to UVB irradiation reduced or completely eliminated UVB-induced formation of thymine dimers and apoptotic cells in the epidermis. These data suggest that nondenatured soymilk could be applied to humans to prevent sunlight-induced skin damage and to reduce the risk of skin tumor formation and progression.
Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8020, USA.
Mou-Tuan
MT
Huang
Jian-Guo
JG
Xie
Connie B
CB
Lin
Menas
M
Kizoulis
Miri
M
Seiberg
Stanley
S
Shapiro
Allan H
AH
Conney
D000287:Administration, Topical; D000818:Animals; D017209:Apoptosis; D004249:DNA Damage; D005260:Female; D006358:Hot Temperature; D051379:Mice; D008812:Mice, Hairless; D009374:Neoplasms, Experimental; D012307:Risk Factors; D012878:Skin Neoplasms / Q000209:etiology* / Q000517:prevention & control* / Q000662:veterinary; D041942:Soy Milk / Q000008:administration & dosage* / Q000494:pharmacology*; D013552:Swine; D014466:Ultraviolet Rays / Q000009:adverse effects