Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid chromatography.
An automated screening method was developed for enzymatic transformations using a robotic system and rapid chiral supercritical fluid chromatography (SFC) analysis with a run time of 1.5 min. The method accelerates the enzyme selection process for screening biocatalysts, where a large number of enzymes are evaluated for activity and enantioselectivity. Kinetic resolution of secondary alcohols by enzymatic transesterification was used as a prototype for method development. The rapid automated method can be used effectively for screening enzymes and optimizing reaction conditions in biocatalysis.
Di
Li
L
Wyeth Research, P.O. Box CN 8000, Princeton, NJ 08543-8000, USA. dil@wyeth.com
McConnell
Oliver J
OJ
Kerns
Edward H
EH
Sutherland
Alan G
AG
D001331:Automation; D001711:Biotransformation; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D012371:Robotics; D013237:Stereoisomerism
D004798:Enzymes