Chemical segregation and reduction of Raman background interference using drop coating deposition.
A new application of the recently described drop coating deposition Raman (DCDR) method facilitates the segregation and independent spectral characterization of mixture components. The quality of the normal (un-enhanced) Raman spectra are significantly improved as a result of reduced spectral interference from fluorescent impurities and buffer compounds. Fluorescence of commercial amino acid (O-phospho-L-serine) and protein (myoglobin) samples is reduced by over an order of magnitude using DCDR, more effectively than prolonged photo-bleaching. Furthermore, DCDR is used to obtain high-quality Raman spectra of proteins, lysozyme, and insulin, derived from solutions with up to 1000-fold excess buffer concentration. Possible thermodynamic and kinetic contributions to the observed segregation phenomena are discussed.
Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, USA.
Dongmao
D
Zhang
Melissa F
MF
Mrozek
Yong
Y
Xie
Dor
D
Ben-Amotz
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D007328:Insulin / Q000032:analysis; D007536:Isomerism; D009113:Muramidase / Q000032:analysis; D009211:Myoglobin / Q000032:analysis; D010768:Phosphoserine / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013816:Thermodynamics
D000596:Amino Acids; D007328:Insulin; D009211:Myoglobin; D010768:Phosphoserine; D009113:Muramidase