MHC expression after human neural stem cell transplantation to brain contused rats.
Human neural stem cells survive and improve motor function after transplantation to the contused brain. However, the transplants might be rejected and that depends on the graft immunogenicity, the host immunological status and the immunosuppression strategy. We transplanted human neural stem cells to rats with brain contusion and analyzed the donor and host MHC antigen expression and the effect of a short-term immunosuppression with cyclosporine. In vitro human neural stem cells expressed only MHC-II antigens. This expression was down-regulated 6 weeks after transplantation. The host response was characterized by an increased MHC-II expression which was down-regulated by a longer term of immunosuppression. These findings are novel and necessary in order to understand the immunogenicity of human neural stem cell grafts.
Department of Clinical Neuroscience, Section of Neurosurgery, Karolinska Institute, Stockholm, Sweden. faiez.al-nimer@kus.se
Faiez
F
Al Nimer
André
A
Wennersten
Staffan
S
Holmin
Xia
X
Meijer
Lars
L
Wahlberg
Tiit
T
Mathiesen
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism* / Q000628:therapy; D002452:Cell Count / Q000379:methods; D053331:Ectodysplasins; D005786:Gene Expression Regulation; D006649:Histocompatibility Antigens / Q000145:classification / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007223:Infant; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013997:Time Factors; D014183:Transplantation, Heterologous
D000943:Antigens, Differentiation; C506018:EDA protein, human; D053331:Ectodysplasins; D006649:Histocompatibility Antigens; D008565:Membrane Proteins