[cDNA microarray analysis of gene expression in acquired methotrexate-resistant of human choriocarcinoma].
OBJECTIVE
To identify the molecular components involved in acquired methotrexate-resistance of human choriocarcinoma.


METHODS
Methotrexate-resistant cell line (JAR/MTX) was derived from JAR cell line by exposed to intervally and progressively increasing higher concentration of MTX. cDNA microarray analyses of JAR/MTX and its parental cell line JAR were performed.


RESULTS
JAR/MTX was established after one year with stable resistance. Its resistant index to MTX was 7.3. Nine genes were differential expressed between JAR/MTX and JAR cells. INSR, SLC1A3, SAT, HBB, and FLJ12443 were underexpressed and HS1, TXNRD1, TAGLN2, and EEF2 were overexpressed in JAR/MTX cells.


CONCLUSIONS
The cDNA microarray system showed that several alterations of gene expression were present in acquired methotrexate-resistance of human choriocarcinoma.
Chen
Ya-xia
YX
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Xie
Xing
X
Cheng
Qi
Q
D017209:Apoptosis; D002448:Cell Adhesion; D002455:Cell Division; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D018076:DNA, Complementary / Q000032:analysis; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis
D018076:DNA, Complementary; D008727:Methotrexate