Carbohydrate metabolism in growing rice seedlings under arsenic toxicity.
We studied in the seedlings of two rice cultivars (Malviya-36 and Pant-12) the effect of increasing levels of arsenic in situ on the content of sugars and the activity of several enzymes of starch and sucrose metabolism: alpha-amylase (EC 3.2.1.1), beta-amylase (EC 3.2.1.2), starch phosphorylase (EC 2.4.1.1), acid invertase (EC 3.2.1.26), sucrose synthase (EC 2.4.1.13) and sucrose phosphate synthase (EC 2.4.1.14). During a growth period of 10-20 d As2O3 at 25 and 50 microM in the growth medium caused an increase in reducing, non-reducing and total soluble sugars. An increased conversion of non-reducing to reducing sugars was observed concomitant with As toxicity. The activities of alpha-amylase, beta-amylase and sucrose phosphate synthase declined, whereas starch phosphorylase, acid invertase and sucrose synthase were found to be elevated. Results indicate that in rice seedlings arsenic toxicity causes perturbations in carbohydrate metabolism leading to the accumulation of soluble sugars by altering enzyme activity. Sucrose synthase possibly plays a positive role in synthesis of sucrose under As-toxicity.
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, India.
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D001151:Arsenic / Q000008:administration & dosage / Q000633:toxicity*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage; D050260:Carbohydrate Metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010087:Oxides / Q000008:administration & dosage / Q000633:toxicity; D013213:Starch / Q000378:metabolism; D024941:Starch Phosphorylase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism; D001614:beta-Amylase / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000378:metabolism
D001152:Arsenicals; D010087:Oxides; D013395:Sucrose; D013213:Starch; D005964:Glucosyltransferases; D024941:Starch Phosphorylase; C022699:sucrose synthase; C022698:sucrose-phosphate synthase; D000516:alpha-Amylases; D001614:beta-Amylase; D043324:beta-Fructofuranosidase; D001151:Arsenic; D000077237:Arsenic Trioxide