Repression of the c-fms gene in fibroblast cells by c-Myc-MM-1-TIF1beta complex.
MM-1 has been reported to repress the E-box-dependent transcription activity of c-Myc by recruiting histone deacetylase 1 complex via TIF1beta/KAP1. In this study, to identify target genes for c-Myc-MM-1-TIF1beta, we established rat-1 cells harboring the dominant-negative form of TIF1beta to abrogate the pathway from TIF1beta to MM-1-c-Myc. This cell line, in which transcription activity of c-Myc was activated, was found to be tumorigenic. By DNA-microarray analysis of this cell line, expression and promoter activity of the c-fms oncogene were found to be upregulated. Of the two promoters, pE1 and pE2, in the c-fms gene, pE1 promoter activity was found to be activated in an E-box-dependent manner.
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
Akiko
A
Satou
Yuko
Y
Hagio
Takahiro
T
Taira
Sanae M M
SM
Iguchi-Ariga
Hiroyoshi
H
Ariga
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D005347:Fibroblasts / Q000502:physiology*; D017930:Genes, Reporter; D016236:Genes, fms / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000494:pharmacology*; D051381:Rats; D012097:Repressor Proteins / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D000076183:Tripartite Motif-Containing Protein 28
D017931:DNA Primers; D004268:DNA-Binding Proteins; C115636:PFDN5 protein, human; D016271:Proto-Oncogene Proteins c-myc; D012097:Repressor Proteins; D008156:Luciferases; C486304:Trim28 protein, rat; D000076183:Tripartite Motif-Containing Protein 28