Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae.
Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity.
Liu
Zhen
Z
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry And Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Yang
Guan-Zhen
GZ
Wu
Xiang-Fu
XF
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002118:Calcium / Q000097:blood; D002478:Cells, Cultured; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D006996:Hypocalcemia / Q000097:blood / Q000139:chemically induced; D007814:Larva / Q000235:genetics*; D007915:Lepidoptera / Q000166:cytology* / Q000235:genetics*; D051379:Mice; D009154:Mutation / Q000235:genetics; D053244:Osteoprotegerin; D020836:Protein Structure, Quaternary; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D014162:Transfection
D006023:Glycoproteins; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C506030:TNFRSF11B protein, human; C506031:Tnfrsf11b protein, mouse; D002118:Calcium