Anomalous diffusion in living yeast cells.
The viscoelastic properties of the cytoplasm of living yeast cells were investigated by studying the motion of lipid granules naturally occurring in the cytoplasm. A large frequency range of observation was obtained by a combination of video-based and laser-based tracking methods. At time scales from 10(-4) to 10(2) s, the granules typically perform subdiffusive motion with characteristics different from previous measurements in living cells. This subdiffusive behavior is thought to be due to the presence of polymer networks and membranous structures in the cytoplasm. Consistent with this hypothesis, we observe that the motion becomes less subdiffusive upon actin disruption.
The Niels Bohr Institute, Blegdamsvej 17, DK-2100 Copenhagen Ø, Denmark.
Iva Marija
IM
Tolić-Nørrelykke
Emilia-Laura
EL
Munteanu
Genevieve
G
Thon
Lene
L
Oddershede
Kirstine
K
Berg-Sørensen
D000199:Actins / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D003593:Cytoplasm / Q000378:metabolism; D004058:Diffusion; D009068:Movement; D012568:Schizosaccharomyces / Q000502:physiology* / Q000648:ultrastructure; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
D000199:Actins