Detection and genetic distance of resistant populations of Pseudosuccinea columella (Mollusca: Lymnaeidae) to Fasciola hepatica (Trematoda: Digenea) using RAPD markers.
Twelve natural populations of Pseudosuccinea columella snails, sampled in the western and central regions of Cuba, were analyzed using the RAPD-PCR technique to screen for resistance to Fasciola hepatica. Ten OPA primers previously shown to produce marker bands for resistance and susceptibility were tested. A new population of P. columella (El Azufre, Pinar del Río) exhibited the amplification patterns of resistant snails, and its resistant status was confirmed after experimental exposure to miracidia. No genetic variability was detected across or within the susceptible isolates. Similarly, the novel resistant isolate displayed an RAPD profile identical to the profile of two other isolates previously identified as resistant to F. hepatica. However, clear differences in RAPD banding patterns and genetic distance were observed between resistant and susceptible isolates.
Laboratorio de Malacología, Instituto Pedro Kouri, IPK. Apartado 601, Marianao 13, Ciudad Habana, Cuba.
Aymé Fernandez
AF
Calienes
Jorge
J
Fraga
Jean-Pierre
JP
Pointier
Mary
M
Yong
Jorge
J
Sanchez
Christine
C
Coustau
Alfredo
A
Gutiérrez
André
A
Théron
D000818:Animals; D003462:Cuba; D005211:Fascioliasis / Q000635:transmission*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D008195:Lymnaea / Q000235:genetics* / Q000469:parasitology*; D019105:Random Amplified Polymorphic DNA Technique