Growth phase-dependent expression of an endoglucanase encoding gene (eglS) in Streptomyces rochei A2.
Different growth phases of Streptomyces rochei A2, a cellulolytic strain isolated from termite gut, were determined in liquid culture. The strain exhibited a multiphasic growth curve typical of streptomycetes. In S. rochei A2, the secreted endoglucanase EglS is coded by a TTA-containing gene, conserved in other Streptomyces. The onset of S. rochei A2 eglS gene expression was determined by RT-PCR using total RNA extracted at different growth phases. The eglS gene was expressed only during transition, second rapid growth and stationary phases, the timing of expression being transcriptionally regulated.
Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Microbiologia e Virologia Generale e Biotecnologie Microbiche, University of Cagliari, Cittadella universitaria SS 554, 09042 Monserrato (CA), Italy.
Elena
E
Tamburini
Brunella
B
Perito
Giorgio
G
Mastromei
D002455:Cell Division; D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D012331:RNA, Fungal / Q000096:biosynthesis; D013302:Streptomyces / Q000166:cytology / Q000201:enzymology* / Q000235:genetics
D012331:RNA, Fungal; D002480:Cellulase