Opposed regulation of aluminum-induced apoptosis by glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor in rat brains.
When intracisternally injected to rat brain, aluminum induced apoptosis as assessed by DNA fragmentation and activation of caspase-3 and caspase-12. Co-administration of glial cell line-derived neurotrophic factor (GDNF) effectively prevented aluminum-induced cell death through reduced apoptosis whereas brain-derived neurotrophic factor (BDNF) accelerated aluminum-induced apoptosis, suggesting that the extent of aluminum neurotoxicity in vivo may depend on the biological activity of the neurotrophic factors.
Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, Songpa-gu, Seoul 138-736, South Korea.
Seung-Ju
SJ
Yang
Jong Eun
JE
Lee
Kwan Ho
KH
Lee
Jae-Wan
JW
Huh
Soo Young
SY
Choi
Sung-Woo
SW
Cho
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D053454:Caspase 12; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004347:Drug Interactions; D051100:Glial Cell Line-Derived Neurotrophic Factor; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009942:Organometallic Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*; D011753:Pyrones / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D019208:Brain-Derived Neurotrophic Factor; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009942:Organometallic Compounds; D011753:Pyrones; C067527:aluminum maltolate; C506443:Casp12 protein, rat; C505410:Casp3 protein, rat; D053454:Caspase 12; D053148:Caspase 3; D020169:Caspases