[Studies on the change of intercellular connection and the appearance and function of ectodesmata-like structure in garlic clove during the dormancy development].
The characteristics of intercellular connection have been observed during the dormancy development of garlic by the transmission electron microscope (TEM). The results indicated that the appearances of the different states of intercellular connection were physiological adaptation to the development of garlic clove during storage. Ectodesmata-like existed at the wall boundary between the declining tissue and the living cells in the garlic during germination period. By Confocal Laser Scanning Microscopy (CLSM) combined with fluorescence probe, 457Da Lucifer Yellow (LYCH) which was impermeable to the membrane, could enter the living parenchyma cell through symplastic route. The result proved that ectodesmata-like structure, which may be recognized as the modified plasmodesata, still retained physiological activity during a certain time and function as the channels of the symplastic transport of nutrient.
Zhang
Ying Ying
YY
College of Life Science, Agricultural University of Hebei, Baoding 071001.
Wang
Dong Mei
DM
Hou
Chun Yan
CY
Han
Sheng Fang
SF
Li
Xiao Juan
XJ
Liu
Gang
G
Liu
Na
N
D005737:Garlic / Q000502:physiology* / Q000648:ultrastructure*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D007365:Intercellular Junctions / Q000502:physiology* / Q000648:ultrastructure*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D031425:Plasmodesmata / Q000502:physiology / Q000648:ultrastructure