Acute phase response in reindeer after challenge with Escherichia coli endotoxin.
The serum concentrations of two acute phase proteins (APPs), haptoglobin (Hp) and serum amyloid-A (SAA), were monitored in reindeer after challenge with endotoxin. Four adult female reindeer received either 0.1 microg/kg Escherichia coli 0111:B4 lipopolysaccharide B or saline solution intravenously. At the second challenge, the treatments were reversed. In addition to the APPs, changes in blood chemistry and rectal temperature were monitored. The endotoxin challenge caused a significant increase in SAA (peak 48 h) and a sharp decrease (8-12 h) of serum iron concentrations in all animals. The mean Hp concentration increased at 8 h and remained elevated until 48 h, but no statistically significant differences were found. This investigation demonstrates that challenge with a single-bolus dose of E. coli endotoxin can activate the acute phase response (APR) and SAA appears to be a more sensitive indicator of the APR than Hp during bacterial infection in reindeer.
Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, P.O. BOX 57, 00014 Helsinki, Finland. toomas.orro@helsinki.fi
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D000210:Acute-Phase Reaction / Q000097:blood / Q000276:immunology*; D000818:Animals; D019446:Endotoxemia / Q000097:blood / Q000276:immunology; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D007501:Iron / Q000097:blood; D012053:Reindeer / Q000276:immunology*; D000685:Serum Amyloid A Protein / Q000378:metabolism
D004731:Endotoxins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; C044101:endotoxin, Escherichia coli; D007501:Iron