[An oligonucleotide primer system for amplification of the ribulose-1,5-bisphosphate carboxylase/oxygenase genes of bacteria of various taxonomic groups].
Based on the analysis of GenBank nucleotide sequences of the cbbL and cbbM genes, coding for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC), the key enzyme of the Calvin cycle, a primer system was designed that allows about 800-bp-long fragments of these genes to be PCR-ampliflied in various photo- and chemotrophic bacteria. The efficiency of the designed primer system in detection of RuBPC genes was demonstrated in PCR with DNA of taxonomically diverse bacteria possessing RuBPC genes with a known primary structure. Nucleotide sequences of RuBPC gene fragments of bacteria belonging to the genera Acidithiobacillus. Ectothiorhodospira, Magnetospirillum, Methylocapsa, Thioalkalispira, Rhodobacter, and Rhodospirillum were determined to be deposited with GenBank and to be translated into amino acid sequences and subjected to phylogenetic analysis.
E M
EM
Spiridonova
I A
IA
Berg
T V
TV
Kolganova
R N
RN
Ivanovskiĭ
B B
BB
Kuznetsov
T P
TP
Turova
D042763:Acidithiobacillus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D020587:Ectothiorhodospira / Q000235:genetics; D020563:Gammaproteobacteria / Q000235:genetics; D005798:Genes, Bacterial; D042201:Magnetospirillum / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012230:Rhizobiaceae / Q000235:genetics; D016992:Rhodobacter / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D017931:DNA Primers; D012273:Ribulose-Bisphosphate Carboxylase