Lignoids in insects: chemical probes for the study of ecdysis, excretion and Trypanosoma cruzi-triatomine interactions.
The effects of phytochemicals (lignans and neolignans) are reviewed in a variety of insect species with special focus on the recent advances on feeding, excretion and Trypanosoma cruzi interactions with Rhodnius prolixus. Burchellin, podophyllotoxin, pinoresinol, sesamin, licarin A, and nordihydroguaiaretic acid (NDGA) added to the diet of Rhodnius prolixus larvae induce antifeedant effects only in doses up to 100 microg/ml of blood meal. Additionally, pinoresinol and NDGA significantly inhibit ecdysis (ED(50)<20 microg/ml). Simultaneous application of ecdysone (1 microg/ml) counteracts ecdysial stasis as induced by NDGA in 5th-instar larvae. Experiments in vivo demonstrate that burchellin and podophyllotoxin (100 microg/ml) diminish excretion post-feeding. Simultaneous treatment with 5-hydroxytryptamine (1 mM, 5-HT), a diuretic hormone, partially reverses this effect of burchellin. Experiments in vitro, using isolated Malpighian tubules of R. prolixus, indicate that burchellin (i) decreases diuretic hormone levels in the hemolymph but not the amount of diuretic hormone stored in the thoracic ganglionic masses (including axons); (ii) reduces the volume of urine secreted by isolated Malpighian tubules; and (iii) 5-HT therapy cannot overcome the effect of burchellin on the Malpighian tubules. In R. prolixus fed on blood containing T. cruzi epimastigotes, the number of parasites in the digestive tract decreases drastically in the presence of burchellin and NDGA (10 microg/ml). When these phytochemicals are applied 20 days after T. cruzi infection, burchellin significantly reduces the gut infection, whereas NDGA does not. However, if the insects are pretreated with both compounds 20 days before subsequent infection with epimastigotes, the parasite infection is almost completely abolished. The same holds true when 5th-instar of R. prolixus are inoculated with 0.5 microg/microl/larva of both neolignans 1 day before infection. Taken together, these findings not only provide a better understanding of the lignoid function in insects, but also offer novel insights into basic physiological processes, which make lignoids interesting candidates for new types of insecticides.
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro 21045-900, RJ, Brazil. egarcia@ioc.fiocruz.br
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D000818:Animals; D001572:Benzofurans; D004149:Dioxoles; D005247:Feeding Behavior / Q000187:drug effects; D005663:Furans; D006790:Host-Parasite Interactions; D007313:Insecta / Q000187:drug effects* / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D017705:Lignans / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D008317:Malpighian Tubules / Q000187:drug effects; D009637:Masoprocol; D018978:Molting / Q000187:drug effects*; D011034:Podophyllotoxin; D012239:Rhodnius / Q000187:drug effects* / Q000469:parasitology / Q000502:physiology; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000502:physiology
D001572:Benzofurans; D004149:Dioxoles; D005663:Furans; D017705:Lignans; C432503:licarin A; C053585:burchellin; D009637:Masoprocol; D011034:Podophyllotoxin; C054125:sesamin; C103298:pinoresinol