Regulated transcripts in the hippocampus following transections of the entorhinal afferents.
Based on the data from a cDNA microarray experiment which was carried out to screen the differential expressed genes in the rat hippocampus 10 days after removal of the entorhinal afferents, we confirmed the increase of expression of eight transcripts encoding protein osteonectin, thymosin-beta4, gelsolin, MHC I, MHC II, beta2-microglobulin, and interferon-gamma receptor using Northern blot. In situ hybridization revealed that the up-regulation of all these 8 transcripts localized specifically in the denervated target areas, the hippocampal stratum lacunosum-moleculare, and the dentate outer molecular layer. The results suggest that these molecules may have roles in the plasticity events in the hippocampus after entorhinal deafferentation.
Key Laboratory of Neurobiology, Shanghai Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
Guo-Xin
GX
Ying
Xin
X
Liu
Wen-Yuan
WY
Wang
Yan
Y
Wang
Jing-Hui
JH
Dong
Hui-Feng
HF
Jin
Cheng
C
Huang
Chang-Fu
CF
Zhou
D000222:Adaptation, Physiological / Q000502:physiology; D000344:Afferent Pathways / Q000166:cytology / Q000378:metabolism* / Q000601:surgery*; D000818:Animals; D018728:Entorhinal Cortex / Q000166:cytology / Q000378:metabolism* / Q000601:surgery*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D014157:Transcription Factors