[Experimental study on p53 gene mutation in keloid fibroblasts].
OBJECTIVE
To explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts.


METHODS
Tissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts.


RESULTS
The points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples.


CONCLUSIONS
p53 gene mutation might play an important role in the formation and development of keloids.
Department of Plastic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P. R. China.
Wang
W
Liu
You-Hui
YH
Jiang
You-Liang
YL
Li
Zi-Hao
ZH
Lin
Hua
H
Jiang
Qian
Q
Tan
Jian-Lin
JL
Zhang
Zi-Min
ZM
Wang
D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D016158:Genes, p53; D006801:Humans; D007627:Keloid / Q000235:genetics*; D008297:Male; D009154:Mutation