Oxysterol regulation of estrogen receptor alpha-mediated gene expression in a transcriptional activation assay system using HeLa cells.
In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor alpha-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17beta-estradiol >> genistein >> beta-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = beta-epoxysitosterol = 7beta-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.
Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan. hiroyosi@junshin-c.ac.jp
Hiroyoshi
H
Sato
Satoko
S
Nishida
Hiroko
H
Tomoyori
Masao
M
Sato
Ikuo
I
Ikeda
Katsumi
K
Imaizumi
D047628:Estrogen Receptor alpha / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans
D047628:Estrogen Receptor alpha; D004967:Estrogens