A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes.
This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.
Plant Gene Expression Center and Plant and Microbial Biology, USDA/ARS and UC-Berkeley, 800 Buchanan St., Albany, CA 94710, USA. sjb@uclink.berkeley.edu
Sheila A
SA
Johnson-Brousseau
Sheila
S
McCormick
D017360:Arabidopsis / Q000235:genetics*; D003433:Crosses, Genetic; D015870:Gene Expression; D010641:Phenotype; D011058:Pollen / Q000235:genetics* / Q000254:growth & development