DNA vaccination of pigs with open reading frame 1-7 of PRRS virus.
We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with ORF7 consistently induced antibodies after three vaccinations, while antibodies were only sporadically detected in the remaining groups. After six vaccinations, all pigs were inoculated with PRRS virus and the post-inoculation antibody response was studied. Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres.
Danish Institute for Food and Veterinary Research, Lindholm, DK-4771 Kalvehave, Denmark. amba@dfvf.dk
Annette Malene
AM
Barfoed
Merete
M
Blixenkrone-Møller
Merethe Holm
MH
Jensen
Anette
A
Bøtner
Søren
S
Kamstrup
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D019470:Biolistics; D003001:Cloning, Molecular; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D009500:Neutralization Tests; D016366:Open Reading Frames / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia / Q000097:blood / Q000276:immunology
D000914:Antibodies, Viral; D017931:DNA Primers; D000939:Epitopes; D019444:Vaccines, DNA; D014765:Viral Vaccines