Co-treatment with ethanol enhances the toxicity of 6-hydroxydopamine.
6-Hydroxydopamine (6-OHDA) is a widely used neural toxin in the pathogenesis research of Parkinson's disease (PD). In this work, we have studied the effect of ethanol on the toxicity of 6-OHDA on PC12 cell and SK-N-SH cell. Ethanol alone had little toxicity to these cells. However, if using 40 microM 6-OHDA along with 400 mM ethanol on PC12 cell or SK-N-SH cell for 24h, there was much more cell loss than using 40 microM 6-OHDA alone when detected by 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay or flow cytometric assay. The toxicity of 6-OHDA was enhanced only if using at least 200 mM ethanol, and the cell loss was increased with the increase of ethanol concentration. We had also found that ethanol could enhance the toxicity of 6-OHDA only when using ethanol and 6-OHDA at the same time, ethanol treatment either before or after 6-OHDA treatment did not show such effect. This effect of ethanol suggests that ethanol may contribute to the degeneration of dopaminergic cells.
Ming
Zhuo
Z
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghia 20031, PR China.
Zhi-shun
Lu
L
Jin-fa
Gu
G
Lan-yin
Sun
S
Xin-yuan
Liu
L
D018663:Adrenergic Agents / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D009447:Neuroblastoma; D016627:Oxidopamine / Q000633:toxicity*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
D018663:Adrenergic Agents; D002492:Central Nervous System Depressants; D013778:Tetrazolium Salts; D013844:Thiazoles; D000431:Ethanol; D016627:Oxidopamine; C022616:thiazolyl blue