Genetic and culture-based approaches for detecting macrolide resistance in Chlamydia pneumoniae.
Three clinical Chlamydia pneumoniae isolates for which the MIC of azithromycin increased after treatment were investigated for genetic evidence of macrolide resistance. Attempts to induce antibiotic resistance in vitro were made. No genetic mechanism was identified for the phenotypic change in these C. pneumoniae isolates. No macrolide resistance was obtained in vitro.
Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA. riska@att.net
Paul F
PF
Riska
Andrei
A
Kutlin
Patrick
P
Ajiboye
Arnold
A
Cua
Patricia M
PM
Roblin
Margaret R
MR
Hammerschlag
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D002690:Chlamydia Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000235:genetics*; D017291:Clarithromycin / Q000494:pharmacology; D017931:DNA Primers; D024881:Drug Resistance, Bacterial; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D018942:Macrolides; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D017963:Azithromycin; D017291:Clarithromycin