[The possibility of using the attenuated recombinant strain of Salmonella enteritidis producing the HBc-antigen as a rectal vaccine in an experiment].
Designing of a therapeutic vaccine for patients with chronic hepatitis B is a task of contemporary medicine. The natural way of administering the vaccine is the most attractive and inexpensive immunization variant. The use of attenuated strain Salmonella as a carrier of the virus antigen fits the purpose. With this idea in mind, the potentialities of the recombinant attenuated strain Salmonella Enteritidis E-23/pKHBc to replicate in the intestine, mesenteric nods and spleen of mice and to stimulate the immune response to HBc-antigen after its rectal administration (as suppositories) in mice were investigated. It was shown that the recombinant strain is discharged from the intestine within 6 hours after immunization, whereas, it is discharged on day 2 after immunization from the lymphatic nods and is traced there during the 5 subsequent days. Besides, the recombinant strain also possessed the possibility to persist in the spleen of mice for 42 days and to induce there the formation of antibodies to HBc-antigen.
Donin
M V
MV
Boĭchenko
M N
MN
Vorob'ev
A A
AA
D000285:Administration, Rectal; D000818:Animals; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016233:Immunotherapy, Active / Q000379:methods; D008221:Lymphoid Tissue / Q000276:immunology / Q000382:microbiology; D051379:Mice; D022562:Salmonella Vaccines / Q000008:administration & dosage* / Q000276:immunology; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D013488:Suppositories; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
D006512:Hepatitis B Core Antigens; D017325:Hepatitis B Vaccines; D022562:Salmonella Vaccines; D013488:Suppositories; D014613:Vaccines, Attenuated