Screening of some Australian Flacourtiaceae species for in vitro antioxidant, cytotoxic and antimicrobial activity.
A total of 27 methanol extracts obtained from different plant parts of 10 species of rain forest trees belonging to four genera of the Flacourtiaceae and originating from Australia were investigated. In vitro cytotoxicity was measured by an ATP Lite-M assay method against the mouse P388 lymphocytic leukemia cell line. The total antioxidant activity has been assessed based on scavenging activity of stable ABTS free radicals. The minimum inhibition concentration (MIC) was determined by the dilution method performed in 96 well plates against four different microbes. The leaf extract of Casearia sp. (RB 3051), mature stem extract of Casearia grayi and stem extract of Scolopia braunii were found to have most antioxidant activity (IC50 = 2.9 microg/ml), cytotoxic activity (LC50 = 0.89 microg/ml) and antimicrobial activity against all four different microbes, respectively. The results obtained suggested that among the four genera studied Casearia is the most promising in respect of finding significant antioxidant, cytotoxic and also antimicrobial activity.
Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore, Australia, Lismore, NSW 2480, Australia. mamosaddik@yahoo.com
M A
MA
Mosaddik
L
L
Banbury
P
P
Forster
R
R
Booth
J
J
Markham
D
D
Leach
P G
PG
Waterman
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001315:Australia; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D008519:Medicine, Traditional; D051379:Mice; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D018547:Plant Stems; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D031308:Salicaceae; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts