DNA replication reaction in Xenopus cell-free system is suppressed by high pressure.
Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.
Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka, 814-80, Japan.
Hitoshi
H
Takahashi
Takeo
T
Yamaguchi
Masaaki
M
Koga
Hiroshi
H
Kageura
Shigeyuki
S
Terada
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D004261:DNA Replication / Q000502:physiology*; D003856:Deoxyuracil Nucleotides / Q000737:chemistry; D005260:Female; D008297:Male; D009865:Oocytes / Q000378:metabolism*; D011312:Pressure / Q000009:adverse effects; D013094:Spermatozoa / Q000378:metabolism*; D014981:Xenopus; D014544:Uridine Triphosphate / Q000031:analogs & derivatives
D001710:Biotin; D003856:Deoxyuracil Nucleotides; C087624:biotin-16-dUTP; D014544:Uridine Triphosphate