[The use of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin and isoniazid].
OBJECTIVE
To study the application of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin (RFP) and isoniazid (INH).


METHODS
Probes were designed and the gene chip was fabricated according to the 30 single nucleotide polymorphisms of 11 mutations on 4 genes associated with RFP and INH resistance. The mutations in Mycobacterium tuberculosis were detected by gene chip to analyze the resistance to INH and RFP.


RESULTS
85 of 110 (77.3%) strains resistant to INH and 22 of 30 (73.3%) strains sensitive to INH were detected, while 77 of 94 (81.9%) strains resistant to RFP and 40 of 46 (87.0%) strains sensitive to RFP were detected. The results from the gene-chip detection were consistent with the sequence information.


CONCLUSIONS
The gene-chip technology, a fast test with high accuracy, specificity and sensitivity, as shown in our study, is promising in the clinical detection of Mycobacterium tuberculosis resistant to INH and RFP.
Cui
Zhen-ling
ZL
Department of Basic Laboratory, Shanghai Pulmonologic Hospital, Shanghai 200433, China.
Jing
Feng-xiang
FX
Hu
Zhong-yi
ZY
Wu
Zi-rong
ZR
Zhao
Jian-long
JL
D024881:Drug Resistance, Bacterial / Q000235:genetics*; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
D007538:Isoniazid; D012293:Rifampin