Imaging of human metaphase chromosomes by atomic force microscopy in liquid.
Human metaphase chromosomes were observed using an intermittent contact mode of atomic force microscopy (AFM) in a phosphate-buffered saline solution to clarify their conformation close to that in the physiological state. In the AFM images in liquid, symmetric alternating ridges and grooves were evident on their surface of the paired sister chromatids. The number of the ridges and grooves were rather specific to the type of the chromosome. The structural changes of chromosomes caused by trypsin treatment were also directly observable using AFM in liquid. These results suggest that the intermittent contact mode AFM is useful not only for analyzing the structure of chromosomes in a liquid condition but also for studying the effect of chemical treatments on chromosomes in relation to their structural changes.
Division of Microscopic Anatomy and Bio-imaging, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Science, Niigata, Japan. o-hoshi@med.niigata-u.ac.jp
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Hoshi
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Owen
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Miles
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Ushiki
D002877:Chromosomes, Human / Q000235:genetics*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008677:Metaphase / Q000235:genetics*; D018625:Microscopy, Atomic Force / Q000379:methods*; D012996:Solutions / Q000737:chemistry
D012996:Solutions