[Effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain].
The effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied. After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days, the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain. The results showed that Cu2+ and Cu-EDTA complex can induce the expression of HSP70 significantly. Comparing with the test groups exposed to Cu2+, the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal. It was found that the fish exposed to the concentration of Cu2+ which below the national fishery water quality standard of 0.01 mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.
Shen
Hua
H
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment Science, Nanjing University, Nanjing 210093, China.
Wang
Xiao-rong
XR
Zhang
Jing-fei
JF
Liu
Hui
H
Zhao
Yong-juan
YJ
D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D003300:Copper; D004492:Edetic Acid