Differentiation of mouse embryonic stem cells into a defined neuronal lineage.
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.
Friedrich Miescher Institute, CH-4058 Basel, Switzerland.
Miriam
M
Bibel
Jens
J
Richter
Katrin
K
Schrenk
Kerry Lee
KL
Tucker
Volker
V
Staiger
Martin
M
Korte
Magdalena
M
Goetz
Yves-Alain
YA
Barde
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D004347:Drug Interactions; D004558:Electric Stimulation / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005136:Eye Proteins; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018756:GABA Antagonists / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000378:metabolism; D051579:Neurogranin; D009474:Neurons / Q000145:classification / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D018408:Patch-Clamp Techniques; D011810:Quinoxalines / Q000494:pharmacology; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000378:metabolism; D018091:Receptors, AMPA / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D012097:Repressor Proteins; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D016708:Synaptophysin; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism; D014633:Valine / Q000031:analogs & derivatives* / Q000494:pharmacology
D001640:Bicuculline; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D005136:Eye Proteins; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018756:GABA Antagonists; D005904:Glial Fibrillary Acidic Protein; D018698:Glutamic Acid; D018398:Homeodomain Proteins; D007211:Indoles; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D051579:Neurogranin; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011810:Quinoxalines; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA; D018091:Receptors, AMPA; D017475:Receptors, Nerve Growth Factor; D012097:Repressor Proteins; D016708:Synaptophysin; D013779:Tetrodotoxin; D014212:Tretinoin; D014404:Tubulin; D014633:Valine; C086936:Fabp7 protein, mouse; C577485:Nes protein, mouse; C496650:Nrgn protein, mouse; C000605732:Pax6 protein, mouse; C462941:beta3 tubulin, mouse; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; C007293:DAPI; C056639:2-amino-5-phosphopentanoic acid