Effects of cyclosporin-A-induced immunosuppression on periapical lesions in rats.
Cyclosporin A (CsA) might induce immune response alterations in periapical lesions and modify bone remodeling. This study determined the changes that occur in the periapical lesions of rats during CsA administration and after CsA withdrawal. After the induction of periapical lesions, the animals were treated with CsA (0-20 mg/kg/day) for 4 wks. Lesion volumes were measured by computed tomography. Histological observations and immunohistochemical evaluations were performed with anti-CD3 and anti-CD25 antibodies. CsA administration reduced lesion volumes, and the lesions significantly expanded after CsA withdrawal. CsA inhibited the proliferation and activation of T-cells at lesion sites. The effects of CsA on T-cells were dose-dependent up to 10 mg/kg/day, after which no significant difference was evident. These results suggest that CsA inhibits periapical destruction by interfering with T-cell function in periapical lesions.
Kawahara
T
T
Department of Restorative Dentistry, Osaka University Graduate School of Dentistry, Yamadaoka, Suita, Japan.
Murakami
S
S
Noiri
Y
Y
Ehara
A
A
Takemura
N
N
Furukawa
S
S
Ebisu
S
S
D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D016723:Bone Remodeling / Q000187:drug effects; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control; D017252:CD3 Complex / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D016572:Cyclosporine / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D015375:Receptors, Interleukin-2 / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects; D014057:Tomography, X-Ray Computed
D000906:Antibodies; D017252:CD3 Complex; D007166:Immunosuppressive Agents; D015375:Receptors, Interleukin-2; D016572:Cyclosporine