Deformation-induced release of ATP from erythrocytes in a poly(dimethylsiloxane)-based microchip with channels that mimic resistance vessels.
The ability of nitric oxide to relax smooth muscle cells surrounding resistance vessels in vivo is well documented. Here, we describe a series of studies designed to quantify amounts of adenosine triphosphate (ATP), a known stimulus of NO production in endothelial cells, released from erythrocytes that are mechanically deformed as these cells traverse microbore channels in lithographically patterned microchips. Results indicate that micromolar amounts of ATP are released from erythrocytes flowing through channels having cross sectional dimensions of 60 x 38 micron (2.22 +/- 0.50 microM ATP). Microscopic images indicate that erythrocytes, when being pumped through the microchip channels, migrate toward the center of the channels, leaving a cell-free or skimming layer at the walls of the channel, a profile known to exist in circulatory vessels in vivo. A comparison of the amounts of ATP released from RBCs mechanically deformed in microbore tubing (2.54 +/- 0.15 microM) vs a microchip (2.59 +/- 0.32 microM) suggests that channels in microchips may serve as functional biomimics of the microvasculature. Control studies involving diamide, a membrane-stiffening agent, suggest that the RBC-derived ATP is not due to cell lysis but rather physical deformation.
Department of Chemistry, Saint Louis University, St. Louis, Missouri 63103, USA.
Alexander K
AK
Price
David J
DJ
Fischer
R Scott
RS
Martin
Dana M
DM
Spence
D000255:Adenosine Triphosphate / Q000097:blood*; D004129:Dimethylpolysiloxanes; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D046208:Microchip Analytical Procedures
D004129:Dimethylpolysiloxanes; D000255:Adenosine Triphosphate