Stimulatory effects of casein phosphopeptide (CPP-III) on mRNA expression of cytokines in Caco-2 cells.
The effect of CPP-III, a commercially available casein phosphopeptide, on the mRNA expression of cytokines in Caco-2 cells was investigated. CPP-III enhanced the mRNAs of interleukin (IL)-6 and tumor necrosis factor-alpha while IL-1beta was not affected. The mRNA expression of IL-6 was stronger in the presence of both CPP-III and bacterial components such as peptidoglycan from Lactobacillus acidophilus and lipopolysaccharide from Salmonella typhimurium. These results suggest that CPP-III influences the expression of cytokines in intestinal epithelial cells.
Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiita, Nagano 399-4598, Japan. tkawafb@shinshu-u.ac.jp
Takeshi
T
Kawahara
Hajime
H
Otani
D018938:Caco-2 Cells; D002364:Caseins / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007779:Lactobacillus acidophilus / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology; D010748:Phosphopeptides / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D002364:Caseins; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D010748:Phosphopeptides; D014409:Tumor Necrosis Factor-alpha