Comparative effects of various nitric oxide donors on ferritin regulation, programmed cell death, and cell redox state in plant cells.
Past studies investigating the regulatory functions of nitric oxide (NO) in plant cells have utilized various NO-donors that release NO in different redox forms, which has lead to problems in the interpretation of data. In the present study, the effects of different NO-donors releasing NO with either NO+ (SNP) or NO' (SNAP, GSNO, NOC-18) character have been compared in plant cells. In particular, ferritin regulation, programmed cell death, cellular redox state, and ROS-scavenging enzymes in Arabidopsis thaliana and Nicotiana tabacum cells were examined. The results show that SNP behaves differently than the other NO-donors tested; indeed, SNP induces accumulation of ferritin transcripts in Arabidopsis, whereas SNAP inhibits its accumulation. Moreover, among the assortment of donors tested, only SNP caused programmed cell death and suppression of ROS-scavenging systems.
Murgia
Irene
I
Sezione di Fisiologia e Biochimica delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, via Celoria, Milano, Italy. irene.murgia@unimi.it
de Pinto
Maria Concetta
MC
Delledonne
Massimo
M
Soave
Carlo
C
De Gara
Laura
L
D017209:Apoptosis / Q000187:drug effects*; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005293:Ferritins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D009569:Nitric Oxide / Q000096:biosynthesis; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D059828:Plant Cells; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
C093285:NOC 18; D020030:Nitric Oxide Donors; D009603:Nitroso Compounds; D017382:Reactive Oxygen Species; C110881:S-nitro-N-acetylpenicillamine; D009599:Nitroprusside; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D005293:Ferritins; D010396:Penicillamine