Effects of monocrotophos and its analogs in acetylcholinesterase activity's inhibition and its pattern of recovery on euryhaline fish, Oreochromis mossambicus.
Acute toxicity of monocrotophos (MCP) and its newly synthesized thiol analogs 2-butenoic acid-3-(diethoxyphosphinothionyl) methyl ester (RPR II) and 2-butenoic acid-3-(diethoxyphosphinothionyl) ethyl ester (RPR-V) was studied on euryhaline fish, Oreochromis mossambicus. The median lethal concentrations of MCP, RPR-II, and RPR-V are 11.506, 0.167, and 0.174 mgL(-1), respectively. Both the analogs were found to be 65-fold more toxic than MCP. Inhibitory and recovery patterns of brain, gill, and muscle acetylcholinesterase (AChE) was studied in vivo after exposure to LC(50) (96 h) concentrations. Recovery study was performed of regular intervals of day-1, -7, -14, -21, and -28 to establish the time course of 50% and 100% recovery in neurotransmitter enzyme of brain, gill, and muscle tissues. The sensitivity of the tissue AChE was in the order gill>brain>muscle. The relative toxicity of these compounds with regard to AChE was RPR-II>RPR-V>MCP. The MCP-treated fishes recovered at a faster rate than for RPR-V, followed by RPR-II.
Rao
J Venkateswara
JV
Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad 500 007, India. jv@iict.res.in
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D005880:Gills / Q000201:enzymology; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008999:Monocrotophos / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity*; D018482:Muscle, Skeletal / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000633:toxicity; D017210:Tilapia / Q000378:metabolism*; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D013438:Sulfhydryl Compounds; C066790:RPR 5; C066789:RPR 2; D008999:Monocrotophos; D000110:Acetylcholinesterase