Correlation of clinical features and methylation status of MGMT gene promoter in glioblastomas.
In an effort to extend the potential relationship between the methylation status of MGMT promoter and response to CENU therapy, we examined the methylation status of MGMT promoter in 44 patients with glioblastomas. Tumor specimens were obtained during surgery before adjuvant treatment, frozen and stored at -80 degrees C until for DNA extraction process. DNA methylation patterns in the CpG island of the MGMT gene were determined in every tumor by methylation specific PCR (MSP). These results were then related to overall survival and response to alkylating agents using statistical analysis. Methylation of the MGMT promoter was detected in 68% of tumors, and 96.7% of methylated tumors exhibited also an unmethylated status. There was no relationship between the methylation status of the MGMT promoter and overall survival and response to alkylating agents. Our observations do not lead us to consider promoter methylation of MGMT gene as a prognostic factor of responsiveness to alkylating agents in glioblastomas.
Blanc
J L
JL
Laboratory of Molecular Oncology, EA 2224, IBMIG, Poitiers, France. l.karayan-tapon@chu-poitiers.fr
Wager
M
M
Guilhot
J
J
Kusy
S
S
Bataille
B
B
Chantereau
T
T
Lapierre
F
F
Larsen
C J
CJ
Karayan-Tapon
L
L
D000368:Aged; D018906:Antineoplastic Agents, Alkylating / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology / Q000532:radiotherapy; D003131:Combined Modality Therapy; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D008875:Middle Aged; D009603:Nitroso Compounds / Q000627:therapeutic use*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000502:physiology; D016896:Treatment Outcome
D018906:Antineoplastic Agents, Alkylating; D004273:DNA, Neoplasm; D009603:Nitroso Compounds; C041148:N-(2-chloroethyl)-N-nitrosoacetamide; D019853:O(6)-Methylguanine-DNA Methyltransferase