The fdh operon of Sulfurospirillum multivorans.
The complete single copy fdh operon (approximately 5.7 kb) encoding the formate dehydrogenase subunits of the gram negative, reductively dehalogenating anaerobe Sulfurospirillum multivorans was sequenced and analyzed. The gene fdhA encoding the catalytically active periplasmic subunit is part of an operon (fdhEABCD) containing additional structural genes. The genes fdhEABCD were cotranscribed as indicated by RT-PCR and primer extension experiments. Two mRNAs for fdhEABCD and fdhABCD were either transcribed independently from two transcription start sites upstream of fdhE and fdhA or might result from posttranscriptional processing of the full-length fdhEABCD mRNA. The operon shows a high degree of similarity to the fdh operons of Campylobacter jejuni and Wolinella succinogenes in terms of architecture and putative cofactor binding motifs of the gene products.
Lehrstuhl für Angewandte und Okologische Mikrobiologie, Institut für Mikrobiologie, FSU Jena, Philosophenweg 12, D-07743 Jena, Germany. roland.schmitz@uni-jena.de
Roland P H
RP
Schmitz
Gabriele
G
Diekert
D000595:Amino Acid Sequence; D020565:Epsilonproteobacteria / Q000201:enzymology / Q000235:genetics*; D005560:Formate Dehydrogenases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D021122:Protein Subunits / Q000235:genetics; D014158:Transcription, Genetic
D021122:Protein Subunits; D005560:Formate Dehydrogenases