Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion.
An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 microL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS(3) was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS(3) ratio is proposed.
Leuthold
Luc Alexis
LA
Laboratory of Pharmaceutical Analytical Chemistry, Life Sciences Mass Spectrometry, School of Pharmacy, University of Geneva, 20 Boulevard d'Yvoy, CH-1211 Geneva 4, Switzerland.
Grivet
Chantal
C
Allen
Mark
M
Baumert
Mark
M
Hopfgartner
Gérard
G
D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004364:Pharmaceutical Preparations