Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.
Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment.
Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
Rong
R
Zeng
Rui-Fu
RF
Yang
Mu-De
MD
Shi
Man-Rong
MR
Jiang
You-Hua
YH
Xie
Hong-Qiang
HQ
Ruan
Xiao-Sheng
XS
Jiang
Lv
L
Shi
Hu
H
Zhou
Lei
L
Zhang
Xiao-Dong
XD
Wu
Ying
Y
Lin
Yong-Yong
YY
Ji
Lei
L
Xiong
Yan
Y
Jin
Er-Hei
EH
Dai
Xiao-Yi
XY
Wang
Bin-Ying
BY
Si
Jin
J
Wang
Hong-Xia
HX
Wang
Cui-E
CE
Wang
Yong-Hua
YH
Gan
Yu-Chuan
YC
Li
Ju-Tian
JT
Cao
Jiang-Ping
JP
Zuo
Shi-Fang
SF
Shan
En
E
Xie
Song-Hua
SH
Chen
Zhi-Qin
ZQ
Jiang
Xi
X
Zhang
Yuan
Y
Wang
Gang
G
Pei
Bing
B
Sun
Jia-Rui
JR
Wu
D000818:Animals; D002522:Chlorocebus aethiops; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D010802:Phylogeny; D040901:Proteomics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
C487105:3a protein, SARS-CoV; D004220:Disulfides; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000086082:Viroporin Proteins