Experimental study on the PAR-1 expression around hemotoma following intracerebral hemorrhage in rats.
In order to explore the PAR-1 mRNA and protein expression around hemotoma following intracerebral hemorrhage and the relation between the PAR-1 expression and thrombin, collagenase VII was stereotaxically injected into right caudate nucleus in rats. The PAR-1 mRNA expression was detected by RT-PCR method and the PAR-1 protein expression by immunohistochemical method respectively. It was found that the PAR-1 mRNA and protein expression around hemotoma was increased at 6 h after intracerebral hemorrhage (P<0.05), peaked at 2 days (P<0.01), and then declined. The change pattern of the PAR-1 mRNA and protein expression was similar to that of intracerebral hemorrhage after thrombin intracerebral injection. The PAR-1 mRNA and protein expression in hirudin group showed no significant difference with control group. These results indicated that the PAR-1 mRNA and protein expression were markedly increased after intracerebral hemorrhage, which may be closely related to thrombin. Upregulation of the PAR-1 expression may involve in neurotoxic injury induced by thrombin.
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Jingxia
J
Guan
Shenggang
S
Sun
Xuebing
X
Cao
Zhibin
Z
Chen
D000818:Animals; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006406:Hematoma / Q000378:metabolism*; D006629:Hirudins / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044463:Receptor, PAR-1 / Q000096:biosynthesis* / Q000235:genetics; D013917:Thrombin / Q000378:metabolism
D005343:Fibrinolytic Agents; D006629:Hirudins; D012333:RNA, Messenger; D044463:Receptor, PAR-1; D013917:Thrombin