Clinical significance of neutrophil CD177 mRNA expression in Ph-negative chronic myeloproliferative disorders.
The PRV-1 gene has been proposed as a marker of polycythaemia vera (PV). PRV-1 and NB1 are alleles of the polymorphic gene CD177, which belongs to the Ly-6/uPAR superfamily, and their coding regions differ at only four nucleotides. We studied neutrophil CD177 mRNA levels in normal subjects and in 235 patients with Ph-negative chronic myeloproliferative disorders (CMD), including PV, essential thrombocythaemia and myelofibrosis with myeloid metaplasia. Additional disease states were investigated for comparison. Highly variable neutrophil CD177 mRNA levels were observed in normal individuals. Neutrophils isolated from the bone marrow, or from peripheral blood following granulocyte colony-stimulating factor administration showed markedly higher CD177 expression than circulating granulocytes on steady state. Increased neutrophil CD177 mRNA levels were detected in all CMD. Elevated values were also found in reactive conditions and in disorders such as chronic myeloid leukaemia and myelodysplastic syndromes. In the differential diagnosis between PV and secondary erythrocytosis, the assay sensitivity was 68% while its specificity was 60%. These findings indicate that an elevated neutrophil CD177 mRNA level is not a specific marker for the diagnosis of PV nor for that of CMD. From a clinical viewpoint, neutrophil CD177 mRNA overexpression is rather a marker of abnormal neutrophil production and/or release in patients with CMD.
Passamonti
Francesco
F
Division of Haematology, University of Pavia Medical School & IRCCS Policlinico S. Matteo, 27100 Pavia, Italy.
Pietra
Daniela
D
Malabarba
Lucia
L
Rumi
Elisa
E
Della Porta
Matteo Giovanni
MG
Malcovati
Luca
L
Bonfichi
Maurizio
M
Pascutto
Cristiana
C
Lazzarino
Mario
M
Cazzola
Mario
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D058851:GPI-Linked Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000235:genetics / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010677:Philadelphia Chromosome; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis*; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases