[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac].
The cell line (BTI-TN-5B1-4) of Trichoplusia ni had been selected by Bacilllus thuringiensis (Bt) toxin Cry1Ac for 86 generations continuously. A high level of resistance was achieved and the partial characterizations of the resistance-selected BTI-TN-5B1-4 cells were studied in order to explore the mechanism of the resistance to Bt toxin. The cells selected by Cry1Ac had low level of cross-resistance (19.7 fold) to Cry1C, their ability of tolerance in the hypotonic solution increased evidently (2.5 fold), and there were differential membrane proteins between resistance-selected and unselected susceptible cells on two dimensional electrophoresis maps. We supposed the changes of membrane proteins in resistant cells would be linked to the cross-resistance to Cry1C and the increase of tolerance in hypotonic solution.
Institute of Entomology, Central China Normal University, Wuhan 430079, China.
Kai Yu
KY
Liu
Hong
H
Yang
Cai Fu
CF
Jiang
Jian Xin
JX
Peng
Hua Zhu
HZ
Hong
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007038:Hypotonic Solutions / Q000494:pharmacology; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D009036:Moths / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007038:Hypotonic Solutions; D019476:Insect Proteins; D008565:Membrane Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis