[Study on biological behavior of osteoblast and vascular endothelial cell culture].
OBJECTIVE
To study the biological behavior of osteoblast and vascular endothelial cell culture.


METHODS
The osteoblasts and vascular endothelial cells were obtained from calvarial bone and renal cortex of 2-week rabbits respectively. The experiment were divided into group A (osteoblasts), group B (vascular endothelial cells) and group C (co-cultured osteoblasts and vascular endothelial cells). The cells were identified with cytoimmunochemical staining. The cellular biological behavior and compatibility were observed under inverted phase contrast microscope and with histological staining. The cells viability and alkaline phosphatase (ALP) activity were measured.


RESULTS
The cytoimmunochemical staining showed that the cultured cells were osteoblasts and vascular endothelial cells. The cellular compatibility of osteoblasts and vascular endothelial cells was good. The ALP activity was higher in group C than in group A and group B (P<0.01), and it was higher in group A than in group B (P<0.05). In group C, the cell proliferation were increased slowly early, but fast later.


CONCLUSIONS
The cellular compatibility of osteoblasts and vascular endothelial cells were good. The vascular endothelial cells can significantly increased the osteoblast viability and ALP activity, and the combined cultured cells have greater proliferation ability.
Rui
Gang
G
Department of Orthopedic Surgery, the First Affiliated Hospital, China Medical University, Shenyang Liaoning, 110001, PR China.
Wang
Zhesheng
Z
Lu
Gang
G
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D011817:Rabbits; D023822:Tissue Engineering
D000469:Alkaline Phosphatase