Characterization of an NBS-LRR resistance gene homologue from soybean.
Conserved motifs such as the nucleotide-binding site (NBS) were found in many characterized plant disease resistance genes. Based on the NBS domain, resistance gene analogs have been isolated in our previous study and were used as probes to screen a soybean (Glycine max) cDNA library. A full-length cDNA, KR4, was isolated by screening the library and rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA was 3818 bp in length and the open reading frame coded for a polypeptide of 1211 amino acids with an NBS and five leucine-rich repeats domains, which were identified by Pfam protein analysis. Sequence alignment showed that KR4 was similar to 12 protein of tomato. Southern analysis indicated that the KR4 gene had low copies in soybean genome and it was mapped on the molecular linkage group E. Its expression was also investigated and it was found that KR4 was induced by exogenous salicylic acid and responded upon infection of soybean mosaic virus strain N3.
Wang
Bangjun
B
Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Wang
Yongjun
Y
Wang
Qiang
Q
Luo
Guangzuo
G
Zhang
Zhigang
Z
He
Chaoying
C
He
Si Jie
SJ
Zhang
Jinsong
J
Gai
Junyi
J
Chen
Shouyi
S
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007113:Immunity, Innate / Q000235:genetics; D000090263:Leucine-Rich Repeat Proteins; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000254:growth & development; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D030262:Soybean Proteins; D019204:GTP-Binding Proteins; D020156:Salicylic Acid