Investigations on the chromatographic behavior of hybrid reversed-phase materials containing electron donor-acceptor systems. I. Contribution of sulfur-aromatic interactions.
The influence of sulfur-aromatic interactions on the chromatographic separation behavior of hybrid RP phases, containing thiol-groups and/or embedded sulfide-groups (S-RP) has been investigated. To allow a precise outline of this new interaction mode, a wide variety of S-RP phases with different alkyl chain length, with and without residual thiol-groups and silanol-endcapping were prepared and tested in comparison to some conventional monomerical as well as polymerical n-alkyl type RP phases. The solute test sets employed in this study comprised the classical chromatographic column tests from Engelhardt and Tanaka as well as test assemblies containing polycyclic aromatic hydrocarbons, stilbene-based cis/trans isomers and functional isomers of benzene. In general, a pronounced strong planar recognition ability as well as a strong increase in the retention for aromatic compounds have been noticed. It was furthermore found that not only the sulfur atom incorporated into the alkyl chain, but also the residual thiol-groups of the 3-propylthiol silica backbone contribute to the overall retention behavior of these novel S-RP type phases.
Horak
Jeannie
J
Institute of Analytical Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria.
Lindner
Wolfgang
W
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004583:Electrons; D011083:Polycyclic Compounds / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur / Q000737:chemistry*
D011083:Polycyclic Compounds; D013455:Sulfur; D012822:Silicon Dioxide