Generation of serotype 1/serotype 2 reassortant viruses of the infectious bursal disease virus and their investigation in vitro and in vivo.
Infectious bursal disease virus (IBDV) is the causative agent of acute or immunosuppressive disease in chickens. Serotype 1 strains are pathogenic whereas serotype 2 strains neither cause disease nor protect against infection with the serotype 1 strains. The target organ of serotype 1 strains is the bursa Fabricii (BF). The molecular determinants of this tropism, and therefore pathogenicity, are poorly understood. IBDV is a non-enveloped icosahedral virus particle of 60 nm in diameter, which contains two genome segments of double-stranded RNA. Here, the generation of interserotypic reassortants using the reverse genetics approach is reported. The results of in vitro and in vivo investigations show that genome segment A determines the bursa tropism of IBDV, whereas segment B is involved in the efficiency of viral replication; they further indicate the significance of the interaction of the polymerase (segment B) with the structural protein VP3 (segment A) or the viral genome for efficient virus formation and replication.
Faculty of Veterinary Medicine, Institute for Virology, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany.
Kati
K
Zierenberg
Rüdiger
R
Raue
Hermann
H
Nieper
Md Rafiqul
MR
Islam
Nicolas
N
Eterradossi
Didier
D
Toquin
Hermann
H
Müller
D000818:Animals; D002060:Bursa of Fabricius / Q000821:virology; D002478:Cells, Cultured; D002645:Chickens; D004195:Disease Models, Animal; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D011485:Protein Binding; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000502:physiology; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology; D015678:Viral Structural Proteins / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
C478244:VP3 protein, infectious bursal disease virus; D014764:Viral Proteins; D015678:Viral Structural Proteins; D012324:RNA-Dependent RNA Polymerase