Typing of Yersinia Enterocolitica Isolates by ITS profiling, REP- and ERIC-PCR.
Thirty-five Yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (ITS) profiling, REP- and ERIC-PCR. ERIC-PCR revealed the presence of seven different genotypes. Amplification of the 16S-23S rDNA spacer region by ITS profiling gave similar results with nine different genotypes. REP-PCR was found to be more discriminatory for typing of Y. enterocolitica than ERIC-PCR and ITS profiling. Fifteen different DNA patterns were obtained by this technique. Based on data obtained by three methods it was found that: (i) Y. enterocolitica strains belonging to the same serotype can represent different genotypes and vice versa; (ii) isolates recovered from humans, pigs and foxes exhibit limited heterogeneity and, independent of the origin, one or two prevailing genotypes were always observed; and (iii) many human Y. enterocolitica isolates shared common genotypes with porcine isolates.
Wojciech
Ł
Ł
Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Agriculture University Norwid St. 31, 50-375 Wroclaw, Poland.
Staroniewicz
Z
Z
Jakubczak
A
A
Ugorski
M
M
D000818:Animals; D016172:DNA Fingerprinting / Q000662:veterinary; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005589:Foxes / Q000382:microbiology; D005838:Genotype; D006801:Humans; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology; D015009:Yersinia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal