[Study on the correlation of interleukin-13 polymorphism and susceptibility to chronic obstructive pulmonary disease in Chinese Han population].
OBJECTIVE
To investigate the polymorphism of interleukin (IL)-13 gene in Chinese Han population, and to study the possible association of IL-13 polymorphism and the susceptibility to chronic obstructive pulmonary disease (COPD).


METHODS
Using genomic DNA extracted with phenol:chloroform:isoamyl alcohol from the blood of 94 healthy smokers and 88 COPD smokers, three single nucleotide polymorphism (SNP) sites in IL-13 gene marked as 1103C/T, 4257G/A, 4738G/A were determined by polymerase chain reaction/restriction fragment length polymorphism analysis or polymerase chain reaction/single strand conformation analysis. In addition, plasma IL-13 concentration was measured by ELISA on 24 healthy smokers and 24 smokers with chronic obstructive pulmonary disease.


RESULTS
(1) To 4257G/A and 4738G/A SNP sites, the genotype frequencies and allele frequencies were not significantly different between healthy smokers and COPD smokers (P > 0.1). (2) To 1103C/T SNP site, relative higher CC genotype frequency and C allele frequency were found in healthy smokers (P < 0.05), also C allele could prevent smokers from chronic obstructive pulmonary disease, with OR 0.56 and 95% confidence interval was 0.34 - 0.91. (3) Plasma IL-13 concentration was not affected by genotype of 4257G/A or 4738G/A SNP sites. However, 1103CC showed for significantly lower IL-13 concentration in plasma (P < 0.05).


CONCLUSIONS
The genotype of IL-13 1103C/T SNP site was associated with the susceptibility to COPD in Chinese Han population and 1103CC accounted for relative lower plasma IL-13 concentration and lower risk for COPD.
Department of Respiratory Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Rui-cheng
RC
Hu
Yong-jian
YJ
Xu
Zhen-xiang
ZX
Zhang
D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005006:Ethnicity; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018793:Interleukin-13 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000235:genetics*; D012307:Risk Factors
D018793:Interleukin-13