Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae.
Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity.
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry And Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Zhen
Z
Liu
Guan-Zhen
GZ
Yang
Xiang-Fu
XF
Wu
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002118:Calcium / Q000097:blood; D002478:Cells, Cultured; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D006996:Hypocalcemia / Q000097:blood / Q000139:chemically induced; D007814:Larva / Q000235:genetics*; D007915:Lepidoptera / Q000166:cytology* / Q000235:genetics*; D051379:Mice; D009154:Mutation / Q000235:genetics; D053244:Osteoprotegerin; D020836:Protein Structure, Quaternary; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D014162:Transfection
D006023:Glycoproteins; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C506030:TNFRSF11B protein, human; C506031:Tnfrsf11b protein, mouse; D002118:Calcium