Effect of trans-resveratrol on the thrombogenicity and atherogenicity in apolipoprotein E-deficient and low-density lipoprotein receptor-deficient mice.
Resveratrol is one of the major polyphenolics in red wine that has been shown to exert the preventive effects against cardiovascular diseases. The effect of trans-resveratrol (t-RES) administered as an ingredient of the diet on the atherothrombotic tendency was assessed in genetically hypercholesterolemic mice after laser-induced damage on endothelium. Mice lacking both apolipoprotein E and low-density lipoprotein receptor (apoE-/-/LDLR-/-) were fed with a high-fat diet with or without t-RES (9.6 and 96 mg/kg diet) for 8 weeks. The atherosclerotic tendency was morphometrically analyzed in their aortae. The thrombotic tendency was determined by inducing thrombus by the irradiation of a helium-neon laser on carotid arteries of these mice with injection of Evans blue. Atherosclerotic area and thrombus size were evaluated by image analyzing in a computer system. Even though the plasma concentrations of lipids (total cholesterol and triacylglycerol) did not change in the control and t-RES groups, a significant decrease (approximately 30%) in the formation of atheroma was observed in the aortae of the t-RES group. The size of laser-induced thrombus that mostly consisted of platelet aggregates was significantly reduced (approximately 25%) in the t-RES group compared with that in the control group. Thus, t-RES orally administrated with a high-fat diet in apoE-/-/LDLR-/- mice significantly suppressed atherosclerosis in their aortae and reduced the laser-induced thrombosis in their carotid arteries.
Department of Nutritional Science, Faculty of Food Culture, Kurashiki Sakuyo University, 3515 Nagao, Tamashima, Kurashiki City, Okayama, 710-0292 Japan. Hideharu.Fukao@fuji.ksu.ac.jp
Hideharu
H
Fukao
Yoshinobu
Y
Ijiri
Mayuko
M
Miura
Masaru
M
Hashimoto
Tsutomu
T
Yamashita
Chizuru
C
Fukunaga
Kazuhiro
K
Oiwa
Yasuhiro
Y
Kawai
Makato
M
Suwa
Junichiro
J
Yamamoto
D000818:Animals; D001018:Aortic Diseases / Q000209:etiology / Q000517:prevention & control*; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D001161:Arteriosclerosis / Q000209:etiology / Q000517:prevention & control*; D002341:Carotid Artery Thrombosis / Q000209:etiology / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D004036:Diet, Atherogenic; D004041:Dietary Fats / Q000008:administration & dosage; D004353:Drug Evaluation, Preclinical; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010974:Platelet Aggregation / Q000187:drug effects / Q000528:radiation effects; D011833:Radiation Injuries, Experimental; D011973:Receptors, LDL / Q000172:deficiency* / Q000235:genetics; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D019851:Thrombophilia / Q000209:etiology / Q000517:prevention & control*; D014280:Triglycerides / Q000097:blood
D001057:Apolipoproteins E; D004041:Dietary Fats; D011973:Receptors, LDL; D013267:Stilbenes; D014280:Triglycerides; D002784:Cholesterol; D000077185:Resveratrol