[Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice].
OBJECTIVE
To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice.


METHODS
Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds.


RESULTS
In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1-3h after incision, and it returned to lower lever by 24h, but increased again at 48h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6h after incision, and the maximum ratio was (51.41 +/- 3.12)% in a 72h-old wound. Then the positive cells gradually decreased (29.38 +/- 2.64)%-(5.56 +/- 4.74)% after incision 96-168h.


CONCLUSIONS
The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.
Zhang
Hui
H
Department of Forensic Pathology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. zhanghui7879@hotmail.com
Zhu
Shao-hua
SH
Qin
Qi-sheng
QS
D000818:Animals; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D012867:Skin / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000503:physiopathology
D016753:Interleukin-10