Respiratory virus infections in Stockholm during seven seasons: a retrospective study of laboratory diagnosis.
A retrospective analysis of the virological findings in all respiratory samples (7303) analysed at the laboratory of Karolinska Hospital between 1993 and 2000 was performed. The findings were studied according to age and seasonal variation, and the methods were evaluated. Most samples were from children. RSV was the dominant agent, found in 34% of all samples from children 0-1 y of age. Influenza A was found in 13% of samples from the age group 2-5 y. Influenza A dominated among adults and the elderly. RSV was found only in 2% of samples from patients 81 y or older. Adenovirus was found among children and adults, but not at all among the elderly. Both antigen detection and virus isolation were performed on 79% (5776) of the samples. For diagnosis of influenza A, virus isolation was more sensitive than immunofluorescence, but for diagnosis of RSV immunofluorescence was more sensitive than virus isolation. Thus, the analysis verified that influenza A is common not only among adults and the elderly, but also among small children. RSV was an uncommon finding among the elderly. Immunofluorescence is sensitive and rapid for the diagnosis of particularly RSV among small children and influenza in all age groups.
Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden. maria.rotzen-ostlund@kus.se
Maria Rotzén
MR
Ostlund
Benita Zweygberg
BZ
Wirgart
Annika
A
Linde
Lena
L
Grillner
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D012189:Retrospective Studies; D012621:Seasons; D013548:Sweden / Q000453:epidemiology; D014776:Virus Cultivation; D014777:Virus Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D014780:Viruses / Q000145:classification / Q000302:isolation & purification*
D000956:Antigens, Viral