[Influence of substrates coated on cultured dorsal root ganglion cells].
Dorsal root ganglia from rat embryos were digested with trypsin to form single cell suspension. The cells were seeded on culture plates coated respectingly with poly-L-lysin (PLL), laminin (LN), PLL combined with LN, or collagen type I (CoI), then cultured in NB1 media. The cell survival and neurite outgrowth of cultured dorsal root ganglion cells were observed by phase-contrast microscopy. The results showed that the dorsal root ganglion neurons grown on PLL combined with LN were dispersively distributed at high survival rate, and those cells grown on CoI were clustered with thicker and longer neurites. It is suggested that the cell growth pattern could be influenced by different substances coated on the plates, and PLL combined with LN may provide a better substrum to culture and study single neuron soma and neurite.
Guo
Li
L
Department of Neurology, the Second Hospital, Hebei Medical University, Shijiazhuang 050000.
Wang
Li Qin
LQ
Song
Xue Qin
XQ
Wang
Xiao Juan
XJ
Li
Chun Yan
CY
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects; D007797:Laminin / Q000494:pharmacology; D008858:Microscopy, Phase-Contrast; D011107:Polylysine / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D007797:Laminin; D011107:Polylysine