Using ultraviolet light for disinfection of finished water.
Ultraviolet light is now recognised to be very effective for inactivation of Cryptosporidium parvum oocysts; however, its application for disinfection of finished water necessitates validation of UV reactors prior to their installation. Although reactor performance will likely be assessed using non-pathogenic microorganisms as biodosimetry surrogates, it would be prudent for the water industry to simultaneously measure Cryptosporidium oocysts inactivation in controlled bench-scale studies using the water matrix intended for disinfection. The likelihood of that occurring is dependent upon the availability of infectivity measurement procedures that are more user-friendly than the mouse infectivity assays currently used. This study describes a modified cell culture procedure that would enable reliable measurement of changes in oocysts' infectivity following their UV treatment. Also, a number of different biodosimetry surrogates were examined and one selected for comparing the UV doses delivered between bench-scale and full-scale biodosimetry studies. Impacts of UV disinfection on production of disinfection byproducts, effects of lamp ageing on effectiveness of disinfection and the costs associated with employing this technology were also examined.
American Water Quality Control and Research Laboratory, Belleville, IL 62220, USA. zbukhari@bellevillelab.com
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Z
Bukhari
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F
Abrams
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M
LeChevallier
D000818:Animals; D003458:Cryptosporidium / Q000472:pathogenicity*; D004203:Disinfection / Q000379:methods*; D033661:Oocysts; D014466:Ultraviolet Rays; D018508:Water Purification / Q000379:methods*