Signals through gp130 upregulate Wnt5a and contribute to cell adhesion in cardiac myocytes.
Glycoprotein 130 (gp130), a common receptor of IL-6 family cytokines, plays critical roles in cardiac functions. Here, we demonstrate that the stimulation of gp130 with leukemia inhibitory factor (LIF) promoted cell adhesion in a cadherin-dependent manner in cultured cardiomyocytes. Wnt5a was upregulated by the stimulation of gp130 with IL-6 family cytokines, accompanied by N-cadherin protein upregulation. Wnt5a was not induced by LIF in cardiomyocytes expressing dominant-negative STAT3. Ablation of Wnt5a by antisense cDNA inhibited LIF-induced cell adhesion. Collectively, signals through gp130 upregulate Wnt5a through STAT3, promoting the N-cadherin-mediated cell adhesion.
Department of Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, 1-6 Yamadaoka, Suita City, Osaka 565-0871, Japan. fujio@phs.osaka-u.ac.jp
Yasushi
Y
Fujio
Takahisa
T
Matsuda
Yuichi
Y
Oshima
Makiko
M
Maeda
Tomomi
T
Mohri
Takashi
T
Ito
Tomoka
T
Takatani
Mayo
M
Hirata
Yoshikazu
Y
Nakaoka
Ryusuke
R
Kimura
Tadamitsu
T
Kishimoto
Junichi
J
Azuma
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130; D016207:Cytokines / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor; D008562:Membrane Glycoproteins / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
D015703:Antigens, CD; D015820:Cadherins; D016207:Cytokines; D004268:DNA-Binding Proteins; C494199:IL6ST protein, human; C494201:Il6st protein, rat; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008562:Membrane Glycoproteins; D011506:Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C494088:Stat3 protein, rat; D015534:Trans-Activators; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C491837:Wnt5a protein, rat; D050822:Cytokine Receptor gp130; C092073:cardiotrophin 1