Hyperosmotically induced accumulation of a phosphorylated p38-like MAPK involved in protoplast volume regulation of plasmolyzed wheat root cells.
A 46 kDa protein resembling immunochemically to the mammalian dually phosphorylated p38-MAPK was detected in wheat root cells under hyperosmotic conditions, using Western blot analysis. This protein accumulated in a time- and dose-dependent fashion and exhibited pharmacological sensitivity similar to the activated p38-MAPK. The application of a highly specific p38-MAPK inhibitor revealed that the p38-like MAPK is probably implicated in hyperosmotically induced tubulin cytoskeleton reorganization as well as in protoplast volume regulation and osmotic tolerance of wheat root cells. As far as we know, the p38-MAPK has not been previously reported in higher plants.
Komis
G
G
Faculty of Biology, Department of Botany, University of Athens, Athens 157 84, Greece.
Apostolakos
P
P
Gaitanaki
C
C
Galatis
B
B
D048429:Cell Size; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D009997:Osmotic Pressure / Q000187:drug effects; D010766:Phosphorylation; D018517:Plant Roots / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D011523:Protoplasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D011725:Pyridines / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014908:Triticum / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D048051:p38 Mitogen-Activated Protein Kinases
D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D013395:Sucrose; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580