Differential compartmentalization of the calpain/calpastatin network with the endoplasmic reticulum and Golgi apparatus.
Calpain, a calcium-activated cysteine protease, is involved in modulating a variety of cell activities such as shape change, mobility, and apoptosis. The two ubiquitous isoforms of this protease, calpain I and II, are considered to be cytosolic proteins that can translocate to various sites in the cell. The activity of calpain is modulated by two regulatory proteins, calpastatin, the specific endogenous inhibitor of calpain, and the 28-kDa regulatory subunit. Using velocity gradient centrifugation, the results of this study confirm and greatly expand upon our previous finding that the calpain/calpastatin network is associated with the endoplasmic reticulum and Golgi apparatus in cells. Moreover, confocal microscopy demonstrates that calpain II colocalizes with specific proteins found in these organelles. Additional experiments reveal that hydrophobic rather than electrostatic interactions are responsible for the association of the calpain/calpastatin network with these organelles. Treatment of the organelles with Na2CO3 or deoxycholate reveal that calpain I, 78-kDa calpain II, and the regulatory subunit are "embedded" within the organelle membranes similar to integral membrane proteins. Proteinase K treatment of the organelles shows that calpain I and II, calpastatin, and the regulatory subunit localize to the cytosolic surface of the organelle membranes, and a subset of calpain II and the regulatory subunit are also found within the lumen of these organelles. These results provide a new and novel explanation for how the calpain/calpastatin network is organized in the cell.
Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky Medical Center, Lexington, Kentucky 40536-0298, USA.
Joshua L
JL
Hood
William H
WH
Brooks
Thomas L
TL
Roszman
D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002154:Calpain / Q000737:chemistry*; D045744:Cell Line, Tumor; D002499:Centrifugation, Density Gradient; D002614:Chelating Agents / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D019286:Endopeptidase K / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008861:Microsomes / Q000378:metabolism; D008954:Models, Biological; D011189:Potassium Chloride / Q000494:pharmacology; D020033:Protein Isoforms; D012965:Sodium Chloride / Q000494:pharmacology; D055672:Static Electricity; D013997:Time Factors; D025801:Ubiquitin / Q000737:chemistry
D002135:Calcium-Binding Proteins; D002614:Chelating Agents; D003902:Detergents; D020033:Protein Isoforms; D025801:Ubiquitin; D003840:Deoxycholic Acid; D012965:Sodium Chloride; D004533:Egtazic Acid; D011189:Potassium Chloride; C033668:calpastatin; D004492:Edetic Acid; D019286:Endopeptidase K; D002154:Calpain