[Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat].
OBJECTIVE
To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells.


METHODS
After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats'viscera were detected by flow cytometry after stained by fluorescence, and also inspect the amount of DNA in different periods to find out the law of its variation.


RESULTS
It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen.


CONCLUSIONS
The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI.
Liu
Zhi-ping
ZP
Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
Chen
Xin
X
She
Yi-wen
YW
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005434:Flow Cytometry; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012394:Rosaniline Dyes; D013154:Spleen / Q000378:metabolism*; D013997:Time Factors
C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA