Microscale transport and sorting by kinesin molecular motors.
As biomolecular detection systems shrink in size, there is an increasing demand for systems that transport and position materials at micron- and nanoscale dimensions. Our goal is to combine cellular transport machinery-kinesin molecular motors and microtubules-with integrated optoelectronics into a hybrid biological/engineered microdevice that will bind, transport, and detect specific proteins, DNA/RNA molecules, viruses, or cells. For microscale transport, 1.5 microm deep channels were created with SU-8 photoresist on glass, kinesin motors adsorbed to the bottom of the channels, and the channel walls used to bend and redirect microtubules moving over the immobilized motors. Novel channel geometries were investigated as a means to redirect and sort microtubules moving in these channels. We show that DC and AC electric fields are sufficient to transport microtubules in solution, establishing an approach for redirecting microtubules moving in channels. Finally, we inverted the geometry to demonstrate that kinesins can transport gold nanowires along surface immobilized microtubules, providing a model for nanoscale directed assembly.
Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Lili
L
Jia
Samira G
SG
Moorjani
Thomas N
TN
Jackson
William O
WO
Hancock
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D004574:Electromagnetic Fields; D004582:Electronics, Medical; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D016547:Kinesins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008870:Microtubules / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D008904:Miniaturization / Q000379:methods; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D009038:Motion; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D010812:Physical Stimulation / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013314:Stress, Mechanical
D020409:Molecular Motor Proteins; D016547:Kinesins