[Proliferation and apoptosis in primary gastrointestinal B-cell non-Hodgkin  s lymphoma and its association with Helicobacter pylori].
OBJECTIVE
To investigate primary gastrointestinal B-cell non-Hodgkin  s lymphoma for clinicopathological features, proliferation, apoptosis and its association with Helicobacter pylori (HP).


METHODS
Classification of tumors, expression of HP, proliferation and apoptosis  related gene products were studied by immunohistochemistry. Apoptosis was studied by TUNEL (TdT [terminal deoxynucleotidyl transferase] dUTP nick end-labeling).


RESULTS
There were 15 cases of mucosa  associated lymphoid tissue (MALT) lymphoma (9 in stomach, 6 in intestine) and 42 cases of diffuse large B cell lymphoma (DLBCL) (28 in stomach, 14 in intestine) in all the 57 cases of primary gastrointestinal BCL. The average apoptotic indexes (AI) were 0.16%, 2.54% in MALT lymphoma and DLBCL respectively. The average proliferation indexes (PI) were 2.22% and 8.71%,respectively. The p53 positive rates were 6.7% and 35.7%. PI (P=0.026) and p53 (P=0.044) had significant differences; But Bcl-2 positive rates were 60.0% and 35.7%,and HP positive rates were 66.7% and 23.8%, respectively. HP had significant differences (P=0.005).


CONCLUSIONS
HP and gastrointestinal MALT lymphoma had significant relation. Apoptosis and proliferative activity were higher in DLBCL than in MALT. The higher Bcl-2 expression in MALT may be related to lower apoptosis. p53 promotes apoptosis and p53 gene mutation may play a role in the progression from MALT to DLBCL. HP antibody may be used in HP detection in gastrointestinal BCL.
Department of Pathology, Peking University First Hospital, Beijing 100034, China. yinhf@hotmail.com
Hong-fang
HF
Yin
Ting
T
Li
Ying
Y
Zhang
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000382:microbiology / Q000473:pathology*; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D007414:Intestinal Neoplasms / Q000382:microbiology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology