Effects of phenylhydrazine or recombinant human erythropoietin on deformability and activity of dehydrogenase glucose-6-phosphate and acetylcholinesterase in Wistar rats blood enriched in reticulocytes.
Deformability and activity of the enzymes: acetylcholinesterase (AChE) and dehydrogenase glucose-6-phosphate (G-6-PD), were assayed for RBC enriched in immature reticulocytes. Reticulocytosis was evoked by administration of two different drugs: recombinant human erythropoietin (rHuEPO) and phenylhydrazine (PHZ) to two groups of Wistar rats. After treatment with the former compound, a group of animals exhibited 17.33% reticulocytes in blood whereas a group of rats treated with the latter drug reached 57.66% of these cells in blood. A marked decrease in RBC deformability was found in both groups of animals. AChE did not significantly change activity neither in PHZ-treated nor in rHuEPO-treated rats, whereas G-6-PD activity was significantly decreased in the PHZ-treated group.
Nowak
Ewa
E
Department of Biochemistry, Agricultural University of Warszawa, Rakowiecka 26/30,02-528 Warszawa, Poland.
Wyrwicz
Grazyna
G
Dabrowski
Zbigniew
Z
Smoleński
Olgierd
O
Spodaryk
Krzysztof
K
D000110:Acetylcholinesterase / Q000187:drug effects* / Q000494:pharmacology*; D000818:Animals; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects* / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D010659:Phenylhydrazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D012156:Reticulocytes / Q000502:physiology*
D016877:Oxidants; D010659:Phenylhydrazines; D011994:Recombinant Proteins; C030299:phenylhydrazine; D004921:Erythropoietin; D005954:Glucosephosphate Dehydrogenase; D000110:Acetylcholinesterase