Regeneration and tolerance factor modulates the effect of adenosine triphosphate-induced interleukin 1 beta secretion in human macrophages.
These studies characterize a molecule known as regeneration and tolerance factor (RTF), which controls inflammation by regulating interleukin 1 beta (IL-1 beta) secretion. Recently, it has been demonstrated that the interaction of adenosine triphosphate (ATP) with the P2X7 purinoceptor induces the secretion of IL-1 beta and initiates the inflammatory response. In these experiments, that the addition of ATP to macrophages was found to induce P2X7 activation and secretion of IL-1 beta. This secretion is enhanced with anti-RTF antibody in combination with exogenous ATP (p< 0.005). RTF is also revealed to be able to influence surface ATPase activity and, increase PI incorporation, which is an indicator of P2X7 activation. We demonstrate that RTF has a role in controlling IL-1 beta secretion by regulating P2X7 activity.
Department of Microbiology and Immunology, Finch University of Health Sciences, The Chicago Medical School, North Chicago, IL 60064, USA.
Richard
R
Derks
Kenneth
K
Beaman
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011419:Propidium / Q000737:chemistry; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058486:Receptors, Purinergic P2X7; D013491:Suppressor Factors, Immunologic / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D014343:Trypan Blue; D006180:Proton-Translocating ATPases
D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate; D000911:Antibodies, Monoclonal; D007371:Interferon-gamma; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011419:Propidium; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D013491:Suppressor Factors, Immunologic; D014343:Trypan Blue; C546468:P2RX7 protein, human; C525845:ATP6V0A2 protein, human; D006180:Proton-Translocating ATPases