Retroviral capsid determinants of Fv1 NB and NR tropism.
The specificity determinants for susceptibility to resistance by the Fv1 n and b alleles map to amino acid 110 of the murine leukemia virus CA protein. To study the interaction between Fv1 and CA, we examined changes in CA resulting in the loss of susceptibility to Fv1 resistance in naturally occurring NB- and NR-tropic viruses. A variety of amino acid changes affecting Fv1 tropism were identified, at CA positions 82, 92 to 95, 105, 114, and 117, and they all were mapped to the apparent exterior of virion-associated CA. These amino acids may form a binding surface for Fv1.
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Anthony
A
Stevens
Michael
M
Bock
Scott
S
Ellis
Paul
P
LeTissier
Kate N
KN
Bishop
Melvyn W
MW
Yap
Willie
W
Taylor
Jonathan P
JP
Stoye
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D041702:BALB 3T3 Cells; D001483:Base Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D014774:Virulence / Q000235:genetics
D036022:Capsid Proteins; D004279:DNA, Viral; C103189:Fv1 protein, mouse; D011506:Proteins; D004247:DNA