Crystal structure of ADP-ribosylated ribosomal translocase from Saccharomyces cerevisiae.
The crystal structure of ADP-ribosylated yeast elongation factor 2 in the presence of sordarin and GDP has been determined at 2.6 A resolution. The diphthamide at the tip of domain IV, which is the target for diphtheria toxin and Pseudomonas aeruginosa exotoxin A, contains a covalently attached ADP-ribose that functions as a very potent inhibitor of the factor. We have obtained an electron density map of ADP-ribosylated translation factor 2 revealing both the ADP-ribosylation and the diphthamide. This is the first structure showing the conformation of an ADP-ribosylated residue and confirms the inversion of configuration at the glycosidic linkage. Binding experiments show that the ADP-ribosylation has limited effect on nucleotide binding affinity, on ribosome binding, and on association with exotoxin A. These results provide insight to the inhibitory mechanism and suggest that inhibition may be caused by erroneous interaction of the translation factor with the codon-anticodon area in the P-site of the ribosome.
Jørgensen
René
R
Macromolecular Crystallography, Department of Molecular Biology, University of Aarhus, Gustav Wieds vej 10C, DK8000 Aarhus, Denmark.
Yates
Susan P
SP
Teal
David J
DJ
Nilsson
Jakob
J
Prentice
Gerry A
GA
Merrill
A Rod
AR
Andersen
Gregers Rom
GR
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000935:Antifungal Agents / Q000378:metabolism; D001665:Binding Sites; D003460:Crystallization; D006153:Guanosine Diphosphate / Q000378:metabolism; D007192:Indenes; D020652:Peptide Elongation Factor 2 / Q000737:chemistry* / Q000378:metabolism; D021381:Protein Transport; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D000935:Antifungal Agents; D007192:Indenes; D020652:Peptide Elongation Factor 2; D029701:Saccharomyces cerevisiae Proteins; C003329:sordarin; D006153:Guanosine Diphosphate; D000246:Adenosine Diphosphate Ribose