Estrogen receptor alpha containing neurons in the monkey forebrain: lack of association with calcium binding proteins and choline acetyltransferase.
The present study used single and dual immunohistochemistry to determine the topography and chemical phenotype of ERalpha containing neurons within the monkey forebrain utilizing antibodies directed against the full-length human ERalpha (NCL-ER-6F11), calcium-binding proteins calbindin-D(28k), and parvalbumin as well as choline acetyltransferase (ChAT). Our findings demonstrate for the first time ERalpha immunoreactive (-ir) cells in the monkey cerebral cortex (layers I-II) and in the claustrum. In addition, ERalpha-ir cells were seen in the septum, basal forebrain, amygdala and hypothalamus. Double-labeled cells for ERalpha and calbindin-D(28k) were seen only in the ventrolateral part of the ventromedial hypothalamic nucleus. In contrast, the co-localization of ERalpha and parvalbumin or ChAT was not seen in any of the areas of the monkey forebrain examined. These observations suggest that estrogens, at least in part, via ERalpha regulate calbindin-D(28k) hypothalamic but not parvalbumin or ChAT containing neurons in select monkey forebrain regions.
Department of Neurological Sciences, Rush University Medical Center, 1735 West Harrison St., Chicago, IL 60612, USA.
Sylvia
S
Perez
T J
TJ
Sendera
J H
JH
Kordower
E J
EJ
Mufson
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis; D002795:Choline O-Acetyltransferase / Q000032:analysis* / Q000096:biosynthesis; D047628:Estrogen Receptor alpha; D005260:Female; D008253:Macaca mulatta; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000378:metabolism; D016548:Prosencephalon / Q000737:chemistry* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis* / Q000096:biosynthesis
D002135:Calcium-Binding Proteins; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen; D002795:Choline O-Acetyltransferase