[Microsatellite instability and loss of heterozygosity of human leucocyte antigen class I gene in cervical carcinoma].
OBJECTIVE
To investigate the frequency of microsatellite instability (MSI) and loss of heterozygosity (LOH) at human leucocyte antigen(HLA) class I loci in cervical carcinoma and the detailed deletion mapping in this region.


METHODS
LOH and MSI of HLA class I genes were analyzed in 30 paired blood and tumor samples by PCR based single-stranded length polymorphism (PCR-SSLP).


RESULTS
Of the 30 cases, 23(76.7%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: C3_2_11 (50%), C1_4_4 (37%), C1_2_5 (36.7%), D6S276 (48.3%). MSI was found in 20 out of 30 cases (66.7%).


CONCLUSIONS
The data suggest that the LOH and MSI of HLA class I gene might participate in the carcinogenesis of cervical carcinoma. Meanwhile, the minimal deletion region might be defined between C1_2_5 and C3_2_11, thus providing the evidence for cloning the tumor suppressor genes associated with cervical carcinoma.
Rong
Yuan
Y
Research Institute of Virology, School of Medicine, Wuhan University, Wuhan, Hubei, 430071 PR China.
Wu
Xu-feng
XF
Chen
Guan-min
GM
Zhao
Min
M
Wu
Xin-xing
XX
D000328:Adult; D000368:Aged; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D053842:Microsatellite Instability; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D015395:Histocompatibility Antigens Class I