Identification of inhibitors for UDP-galactopyranose mutase.
The flavoenzyme uridine 5'-diphosphate (UDP)-galactopyranose mutase (UGM) plays a key role in the cell wall biosynthesis of many pathogens, including Mycobacterium tuberculosis. Using a synthetic fluorescent ligand, we screened 16 000 compounds in a fluorescence polarization assay. Effective inhibitors of UGM were identified.
Soltero-Higgin
Michelle
M
Departments of Biochemistry and Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Carlson
Erin E
EE
Phillips
John H
JH
Kiessling
Laura L
LL
D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005454:Fluorescence Polarization / Q000379:methods; D020128:Inhibitory Concentration 50; D019751:Intramolecular Transferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007700:Kinetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D019751:Intramolecular Transferases; C098051:UDP-galactopyranose mutase