NAD-sensitive hydrogel for the release of macromolecules.
A hydrogel membrane containing immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and hemoglobin). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked to dextran molecules that were subsequently crosslinked to form a gel. The resulting stable hydrogels contained both covalent and affinity crosslinks such that their intrinsic porosities were sensitive to competitive displacers of the affinity interaction between lysozyme and Cibacron blue. Transport experiments in a twin chamber diffusion cell showed that as NAD was added to the donor side, the dissociation of the binding sites between the Cibacron blue and the lysozyme led to an increase in protein diffusion through the hydrogel. The results showed that addition of NAD caused a saturable concentration-dependent increase in the transport of both cytochrome C and hemoglobin. This effect was shown to be both specific and reversible.
Department of Chemical Engineering, University of Bath, UK.
M
M
Tang
R
R
Zhang
A
A
Bowyer
R
R
Eisenthal
J
J
Hubble
D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D045304:Cytochromes c / Q000008:administration & dosage / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods; D006454:Hemoglobins / Q000008:administration & dosage / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000008:administration & dosage / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D014677:Pharmaceutical Vehicles / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D006454:Hemoglobins; D020100:Hydrogels; D046912:Multiprotein Complexes; D014677:Pharmaceutical Vehicles; D009243:NAD; D045304:Cytochromes c