N-acetylcysteine in intestinal reperfusion injury: an experimental study in rats.
BACKGROUND
The aim of the present study was to investigate the effect of N-acetylcysteine on intestinal reperfusion injury.


METHODS
Forty Sprague-Dawley rats were divided into four groups (n = 10): sham, sham + N-acetylcysteine, reperfusion, and reperfusion + N-acetylcysteine. Thirty minutes of ischaemia +/- 30 min of reperfusion was performed under 100 mg/kg N-acetylcysteine or placebo, administered 30 min before the operation in the groups where appropriate. Ileum samples were resected for histopathologic evaluation and tissue malondialdehyde and super oxide dismutase level determination.


RESULTS
The mean mucosal injury score and malondialdehyde level of the reperfusion and reperfusion + N-acetylcysteine groups were significantly higher than that of the control and control + N-acetylcysteine group (P < 0.01, P < 0.05, respectively). Mean super oxide dismutase level of the control + N-acetylcysteine group was significantly higher than that of the other groups (P < 0.05).


CONCLUSIONS
N-Acetylcysteine did not prevent intestinal reperfusion injury by means of histopathologic findings and malondialdehyde level.
Department of General Surgery,Medical School, Ankara University, 06170 Ankara, Turkey. selcukhazinedaroglu@yahoo.com
Selcuk M
SM
Hazinedaroglu
Ferda
F
Dulger
Huseyin A
HA
Kayaoglu
Mevlut
M
Pehlivan
Ebru
E
Serinsoz
Orhan
O
Canbolat
Nezih
N
Erverdi
D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D007082:Ileum / Q000201:enzymology / Q000473:pathology; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000111:Acetylcysteine