Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments.
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.
Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Torstein
T
Tengs
Thomas
T
LaFramboise
Robert B
RB
Den
David N
DN
Hayes
Jianhua
J
Zhang
Saikat
S
DebRoy
Robert C
RC
Gentleman
Keith
K
O'Neill
Bruce
B
Birren
Matthew
M
Meyerson
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D019295:Computational Biology; D003198:Computer Simulation; D044708:DNA, Concatenated / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D015894:Genome, Human; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008390:Markov Chains; D016133:Polymerase Chain Reaction; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification
D044708:DNA, Concatenated; D015252:Deoxyribonucleases, Type II Site-Specific