Expression of tetraspanins in peripheral blood leukocytes: a comparison between normal and infectious conditions.
The role of tetraspanins is undefined, despite their detection in diverse cell types and functions. This study addresses the characterization of tetraspanin expression levels in normal peripheral blood leukocytes (PBL) and in patients with bacterial infection. Membranal and cytoplasmic expression of CD9, CD53, CD63, CD81, CD82 and CD151 in polymorphonuclears (PMN), monocytes, B and T lymphocytes was assessed using flow cytometry. Results suggested that for normal PBL, PMN are distinguished by dominant cytoplasmic CD63; monocytes and B cells prevailingly express CD53; CD82 is primarily expressed on T-cell membranes. However, a major trend of downregulation was demonstrated for the examined tetraspanins, except CD63, in all patients' PBL subtypes. Therefore, tetraspanin modulation in infections may be attributed to elevated leukocyte motility in immune reactions and this is compatible with the previous publications of tetraspanins as metastasis suppressors. This work represents the first comprehensive baseline of tetraspanin expression in normal PBL and in infectious disorders.
Oncogenetic Laboratory, Sapir Medical Center, Meir Hospital, Kfar Saba, Israel.
T
T
Tohami
L
L
Drucker
J
J
Radnay
H
H
Shapira
M
M
Lishner
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D051861:Kangai-1 Protein; D007962:Leukocytes / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000235:genetics; D011014:Pneumonia / Q000276:immunology / Q000503:physiopathology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D060185:Tetraspanin 24; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D060245:Tetraspanin 29; D060149:Tetraspanin 30; D014552:Urinary Tract Infections / Q000276:immunology / Q000503:physiopathology*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C554715:CD151 protein, human; C554894:CD53 protein, human; C554586:CD63 protein, human; C554798:CD81 protein, human; C497688:CD82 protein, human; C554869:CD9 protein, human; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins; D011518:Proto-Oncogene Proteins; D060185:Tetraspanin 24; D060265:Tetraspanin 25; D060225:Tetraspanin 28; D060245:Tetraspanin 29; D060149:Tetraspanin 30