Detection of trophic factor activated signaling molecules in cells by a compact fiber-optic sensor.
This paper describes a highly sensitive method to detect trophic factor activated signaling molecules in cells using a compact fiber optic biosensor. The method is demonstrated by quantitative detection of phosphorylation of signal transducers and activators of transcription 3 (STAT3) in neuroblastoma cells. A single fiber-optic probe based on total internal reflection fluorescence sensing system is used. A 405 nm diode laser is used for evanescent wave excitation of immobilized labelled analyte on the probe surface. A compact charged coupled device (CCD) based spectrometer is used for recording the fluorescence signal. The method is two orders of magnitude more sensitive than the Western blotting technique.
Institute for Lasers, Photonics and Biophotonics, University at Buffalo, State University of New York, 458 NSC, Buffalo, NY 14260, USA. rkapoor@acsu.buffalo.edu
Rakesh
R
Kapoor
Navjot
N
Kaur
Emmanuel T
ET
Nishanth
Stanley W
SW
Halvorsen
Earl J
EJ
Bergey
Paras N
PN
Prasad
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D009447:Neuroblastoma / Q000175:diagnosis / Q000378:metabolism*; D055100:Optical Fibers; D017978:Receptors, Growth Factor / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results; D050796:STAT3 Transcription Factor; D012680:Sensitivity and Specificity; D015398:Signal Transduction; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism*
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D017978:Receptors, Growth Factor; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators