Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis.
BACKGROUND
Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population.


METHODS
We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method.


RESULTS
We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children.


CONCLUSIONS
Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
Nakamura
Hiroyuki
H
Department of Environmental Medicine, Kochi University School of Medicine, Kohasu, Okoh-cho, Nankoku 783-8505, Japan. hiro-n@po.incl.ne.jp
Higashikawa
Fumiko
F
Miyagawa
Kiyoshi
K
Nobukuni
Yoshitaka
Y
Endo
Tomohiko
T
Imai
Toru
T
Ozasa
Kotaro
K
Motohashi
Yutaka
Y
Matsuzaki
Ichiyo
I
Sasahara
Shinichiro
S
Hatta
Kotaro
K
Ogino
Keiki
K
Eboshida
Akira
A
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D002648:Child; D032485:Cryptomeria / Q000276:immunology; D047209:Eosinophil Peroxidase; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D010544:Peroxidases / Q000235:genetics*; D011058:Pollen / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
D000485:Allergens; D047209:Eosinophil Peroxidase; D010544:Peroxidases