Genetic transformation of mature citrus plants.
Most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. Evaluation of characteristics related to fruits is a requisite to release any new variety into the market. Because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. Direct transformation of mature material could ensure the production of adult transgenic plants, bypassing in this way the juvenile phase. Invigoration of the source adult material, establishment of adequate transformation and regeneration conditions, and acceleration of plant development through grafting allowed us to produce transgenic mature sweet orange trees flowering and bearing fruits in a short time period.
Department of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain.
Magdalena
M
Cervera
José
J
Juárez
Luis
L
Navarro
Leandro
L
Peña
D016960:Agrobacterium tumefaciens / Q000235:genetics; D002478:Cells, Cultured; D002957:Citrus / Q000235:genetics* / Q000254:growth & development; D046508:Culture Techniques / Q000379:methods; D005638:Fruit / Q000235:genetics; D007202:Indicators and Reagents; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
D007202:Indicators and Reagents