The central half of Pit2 is not required for its function as a retroviral receptor.
The type III sodium-dependent phosphate (NaPi) cotransporter, Pit2, is a receptor for amphotropic murine leukemia virus (A-MuLV) and 10A1 MuLV. In order to determine what is sufficient for Pit2 receptor function, a deletion mutant lacking about the middle half of the protein was made. The mutant supported entry for both viruses, unequivocally narrowing down the identification of the sequence that is sufficient to specify the receptor functions of Pit2 to its N-terminal 182 amino acids and C-terminal 170 amino acids.
Bøttger
Pernille
P
Department of Molecular Biology, Aarhus University, Aarhus C, Denmark.
Pedersen
Lene
L
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D009052:Leukemia Virus, Murine / Q000378:metabolism* / Q000502:physiology; D011991:Receptors, Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics
D011991:Receptors, Virus; C067981:leukemia virus receptor, gibbon ape