[Cytotoxicity of chymopapain combined with pingyangmycin on mouse hepatoma cell line hepa-6].
OBJECTIVE
To observe the cytotoxicity of chymopapain combined with pingyangmycin (PYM) on mouse hepatoma cell line hapa-6 in vitro.


METHODS
MTT method.


RESULTS
Chymopapain showed cytotoxicities to hepa-6 (IC50 is about 1200 microg/ml) and had concentration dependency. Cooperation effect was showed when three different concentration chymopapain combined with PYM during 48 hours. It accorded with the change of enzyme activity of chymopapain in saline water. Cooperation effect was showed again when adding chymopapin for the second time at 48 hours.


CONCLUSIONS
Chymopapain has cytotoxicity on hepa-6 in vitro. Cooperation effect was showed when chymopapin was combined with PYM.
General Hospital of Guangzhou Military Command, Guangzhou 510010.
Cai
C
Xiaoling
Wang
W
Jie
Guo
G
Yong
Yang
Y
Taicheng
Xian
X
Jiang
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002917:Chymopapain / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D010206:Papain / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D004365:Drugs, Chinese Herbal; D001761:Bleomycin; C021771:zhengguangmycin; D010206:Papain; D002917:Chymopapain