Suppressive effects of ascorbate derivatives on ultraviolet-B-induced injury in HaCaT human keratinocytes.
The aging of skin, including sunburning, is caused by ultraviolet (UV) irradiation. Here, we examined the inhibitory effect of ascorbic acid (AsA) and its derivatives AsA 2-phosphate (AA-2P) and AsA 2-glucoside (AA-2G) on UV-B- induced cytotoxicity in HaCaT keratinocytes. Results show that cell viability significantly decreased when exposed to UV-B at 0.1-0.4 J/cm2 in a dose-dependent manner. In this study, AsA could not inhibit cytotoxicity, but AA-2P and AA-2G was able to cancel the harmful effect of UV-B when treated at high levels of 0.5-5 mM. These results indicate that the masking of the C-2 OH group may be an effective modification for AsA to inhibit UV-B-induced cytotoxicity in human keratinocytes.
Laboratory of Food Biological Chemistry, Faculty of Agriculture, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-0082, Japan.
Shin
S
Yasuda
Mikiro
M
Tada
Koji
K
Yamada
Kyoya
K
Takahata
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; C011669:ascorbate-2-phosphate; C065378:ascorbic acid 2-O-glucoside; D001205:Ascorbic Acid