A new approach to natural products discovery exemplified by the identification of sulfated nucleosides in spider venom.
Using a new approach based on the NMR spectroscopic analysis of the entire, unpurified spider venom, we identified a family of unusual sulfated nucleoside derivatives from the venom of the hobo spider, Tegenaria agrestis. These compounds are ribonucleoside mono- and disulfates derived from guanosine and xanthosine, some of which are glycosylated, bearing one or two D-fucose units. The use of NMR spectroscopy to characterize the unfractionated venom was central to the discovery of this heretofore overlooked class of venom components.
Taggi
Andrew E
AE
Contribution from The Cornell Institute for Research in Chemical Ecology Baker Laboratory, Cornell University, Ithaca, NY 14853, USA.
Meinwald
Jerrold
J
Schroeder
Frank C
FC
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry*; D013111:Spider Venoms / Q000737:chemistry*; D013112:Spiders / Q000378:metabolism; D013431:Sulfates / Q000032:analysis / Q000737:chemistry*
D009705:Nucleosides; D013111:Spider Venoms; D013431:Sulfates