[Inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma].
OBJECTIVE
To evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.


METHODS
The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT.


RESULTS
The expression of PTEN protein was induced on day 1, and greatly increasing on day 3 - 5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-II.


CONCLUSIONS
Adenovirus-mediated introduction of exogenous PTEN into human endometrial carcinoma cells can induce growth suppression. PTEN gene may be a novel therapeutic agent for endometrial carcinoma.
Wan
Xiao-yun
XY
Woman's Hospital, Zhejiang University, Hangzhou 310006, China. xiaoyunw@yahoo.com
Shi
Yi-fu
YF
Li
Juan-qing
JQ
D000256:Adenoviridae / Q000235:genetics*; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D011995:Recombination, Genetic; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
D025521:Tumor Suppressor Proteins; D007335:Insulin-Like Growth Factor II; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human