Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli.
Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 micromol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 micromol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively. Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.
Bioprocessing Group, Environmental Technological Division, Pacific Northwest National Laboratory, P.O. Box 999, MSIN K2-10, Richland, WA 99352, USA. ziyudai@pnl.gov
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Dai
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Hooker
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Quesenberry
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Gao