Label free novel electrical detection using micromachined PZT monolithic thin film cantilever for the detection of C-reactive protein.
In this paper, we report the novel electrical measurement for the label-free detection of C-reactive protein (CRP) using resonant frequency shift in the monolithic thin film cantilever of micromachined Pb(Zr0.52Ti0.48)O3 (PZT) which was fabricated with the composition of SiO2/Ta/Pt/PZT/Pt/SiO2 on silicon nitride (SiNx) supporting layer for the dual purpose of electrical self-excitation and sensing. The specific binding characteristics of CRP antigen to its antibody, which is immobilized with Calixcrown SAMs on Au surface deposited on microcantilever, is determined in high sensitivity to the nanogram level per milliliter by measuring the resonant frequency shift. The nanomechanical PZT cantilever turns out a robust platform for the highly specific antigen-antibody interaction and provides with the novel tool for qualification and quantification of biomolecules without any sample labeling and bulky optical apparatus.
Lee
Jeong Hoon
JH
Microsystem Research Center, 39-1 Haweolgok-dong, Seongbuk-gu, KIST, Seoul 136-791, South Korea.
Yoon
Ki Hyun
KH
Hwang
Kyo Seon
KS
Park
Jaebum
J
Ahn
Saeyoung
S
Kim
Tae Song
TS
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002097:C-Reactive Protein / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007854:Lead / Q000737:chemistry*; D008420:Manufactured Materials; D008567:Membranes, Artificial; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014025:Titanium / Q000737:chemistry*; D014159:Transducers; D015040:Zirconium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; C065536:lead titanate zirconate; D007854:Lead; D002097:C-Reactive Protein; D015040:Zirconium; D014025:Titanium