[Increased expression of heparanase mRNA is correlated with metastasis of gastric carcinoma and is probably correlated with c-met protein].
OBJECTIVE
To explore the expression of human heparanase mRNA and its relationship with the clinicopathological characteristics and angiogenesis and its possible mechanism in gastric carcinomas.


METHODS
Expression of heparanase mRNA in gastric cancerous tissues and its corresponding adjacent tissues were detected by RT-PCR in forty-seven patients. Expression of c-met protein and microvessel density (MVD) were examined by immunohistochemcal staining.


RESULTS
Expression of heparanse mRNA was significantly higher in gastric cancerous tissues than in its adjacent tissues (74.5% vs. 19.1%, P < 0.01). MVD was significantly higher in heparanase positive group than its counterpart (51 +/- 15 vs. 31 +/- 10, P < 0.01). Expression of heparanase mRNA was correlated with expression of c-met (r = 0.714, P < 0.01). The positive rate of heparanse expression in gastric carcinomas were correlated with tumor diameter, invasive depth, regional lymph nodes metastasis and TNM stage, whereas there were no relation with gender, age, tumor differentiation and remote metastasis of the patient.


CONCLUSIONS
Increased heparanase expression contributes to metastasis and angiogenesis in gastric cancer. Heparanase mRNA expression correlates with poor clinicopathologic characteristics in its prognosis. C-met is correlated with heparanase expression.
The Third Military South West Hospital, Repartarment of Gastrointes-tinal, Chongqing 400038, China.
Yong-guo
YG
Cai
Dian-chun
DC
Fang
Shi-ming
SM
Yang
Yuan-hui
YH
Luo
Meng-hua
MH
Yang
Dong-xu
DX
Wang
D000328:Adult; D000368:Aged; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit; D009389:Neovascularization, Pathologic; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D000099175:Heparanase
D005966:Glucuronidase; D052002:NF-kappa B p50 Subunit; D019859:Proto-Oncogene Proteins c-met; D012333:RNA, Messenger; D014157:Transcription Factors; D000099175:Heparanase; C489548:NFKB1 protein, human