In vitro expression of full-length and truncated bovine respiratory syncytial virus G proteins and their antibody responses in BALB/c mice.
Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, a significant and equivalent anti-BRSV G IgG response was elicited to the full-length and truncated BRSV G proteins. These constructs may be used to study host pathological and immunological responses.
Department of Veterinary and Biomedical Sciences, University of Nebraska, East Campus Loop and Fair Street, Veterinary Basic Science, Lincoln, NE 68583-0905, USA.
Ryan P
RP
Brady
Christina L
CL
Topliff
Clayton L
CL
Kelling
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D019556:COS Cells; D002417:Cattle; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology* / Q000378:metabolism*; D014162:Transfection; D022223:Vaccines, Subunit / Q000096:biosynthesis / Q000276:immunology; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D004247:DNA; D019204:GTP-Binding Proteins