Differential regulation of biglycan and decorin synthesis by connective tissue growth factor in cultured vascular endothelial cells.
It is possible that connective tissue growth factor (CTGF) serves as either an independent regulator or a downstream effector of transforming growth factor-beta (TGF-beta) on the proteoglycan synthesis in vascular endothelial cells. Since TGF-beta regulates endothelial proteoglycan synthesis in a cell density-dependent manner, dense and sparse cultures of bovine aortic endothelial cells were metabolically labeled with [(35)S]sulfate or (35)S-labeled amino acids in the presence of CTGF, and the labeled proteoglycans were characterized by biochemical techniques. The results indicate that CTGF suppresses the synthesis of biglycan but newly induced that of decorin in the cells when the cell density is low; in addition, no change was observed in the hydrodynamic size and the glycosaminoglycan chain length of these two small chondroitin/dermatan sulfate proteoglycans. The regulation of endothelial proteoglycan synthesis by CTGF is completely different from that by TGF-beta, suggesting that CTGF is not a downstream effector of TGF-beta but an independent regulator in vascular endothelial cells with respect to the proteoglycan synthesis.
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan. t-kaji@hokuriku-u.ac.jp
Toshiyuki
T
Kaji
Chika
C
Yamamoto
Mami
M
Oh-i
Takashi
T
Nishida
Masaharu
M
Takigawa
D000595:Amino Acid Sequence; D000818:Animals; D058578:Biglycan; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor; D058575:Decorin; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor