Phototoxicity against tumor cells and Sindbis virus by an octahedral rhodium bisbipyridyl complex and evidence for the genome as a target in viral photoinactivation.
An octahedral rhodium complex (cis-dichloro(dipyrido[3,2a-2',3'c]phenazine)(1,10-phenanthroline)rhodium(III) chloride; DPPZPHEN) has been prepared that can penetrate tumor cell membranes and the Sindbis viral capsid. The compound is phototoxic to these entities when irradiated with UVA light. Model studies with calf thymus and supercoiled plasmid DNA indicate that the complex can both bind with, and nick, nucleic acid. Analysis of Sindbis virus, following irradiation with the metal complex, confirmed that the viral genome was rendered noninfectious by this treatment.
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
Elton L
EL
Menon
Rushika
R
Perera
Maribel
M
Navarro
Richard J
RJ
Kuhn
Harry
H
Morrison
D000818:Animals; D002417:Cattle; D004249:DNA Damage; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D010782:Photolysis; D010957:Plasmids / Q000528:radiation effects; D012238:Rhodium / Q000737:chemistry*; D012845:Sindbis Virus / Q000528:radiation effects*; D013950:Thymus Gland / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D009942:Organometallic Compounds; C490279:cis-dichloro(dipyrido(3,2a-2',3'c)phenazine)(1,10-phenanthroline)rhodium(III) chloride; D012238:Rhodium