A new approach to natural products discovery exemplified by the identification of sulfated nucleosides in spider venom.
Using a new approach based on the NMR spectroscopic analysis of the entire, unpurified spider venom, we identified a family of unusual sulfated nucleoside derivatives from the venom of the hobo spider, Tegenaria agrestis. These compounds are ribonucleoside mono- and disulfates derived from guanosine and xanthosine, some of which are glycosylated, bearing one or two D-fucose units. The use of NMR spectroscopy to characterize the unfractionated venom was central to the discovery of this heretofore overlooked class of venom components.
Contribution from The Cornell Institute for Research in Chemical Ecology Baker Laboratory, Cornell University, Ithaca, NY 14853, USA.
Andrew E
AE
Taggi
Jerrold
J
Meinwald
Frank C
FC
Schroeder
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry*; D013111:Spider Venoms / Q000737:chemistry*; D013112:Spiders / Q000378:metabolism; D013431:Sulfates / Q000032:analysis / Q000737:chemistry*
D009705:Nucleosides; D013111:Spider Venoms; D013431:Sulfates