Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli.
Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 micromol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 micromol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively. Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.
Dai
Z
Z
Bioprocessing Group, Environmental Technological Division, Pacific Northwest National Laboratory, P.O. Box 999, MSIN K2-10, Richland, WA 99352, USA. ziyudai@pnl.gov
Hooker
B S
BS
Quesenberry
R D
RD
Gao
J
J