[Establishment and characterization of a neural stem cell line (RNSC-FMU 1) from rat brain].
A neural stem cell line (RNSC-FMU 1) from rat brain have been established successfully by isolating and culturing neural stem cells from newborn SD rat brain in vitro with free-serum medium and passaging with mechanical division. The cell line cultured can continuously generate in vitro for long-term and it is 21 months (>100 passages) so far. These cells keep the feature of neural stem cell and normal karyotype. These neural stem cells can be induced to differentiate into neurons, astrocytes and oligodendrocytes. The cells have an extensive self-renewal capacity; its doubling time of proliferation is about 20 h. The cells are also cryopreservable. Tumor formation is not observed in nude mice that explanted with the cells. This cell line is a good tool for research of neural stem cell.
Zheng
Zhi Hong
ZH
Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 350004.
Hu
Jian Shi
JS
Lin
Ling
L
You
Xiao Qing
XQ
Zhang
Qi
Q
Lin
Jian Yin
JY
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D015925:Cryopreservation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009474:Neurons / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology*