[Preparation of magnetoliposomes and its in vivo behavior on ICR mice].
OBJECTIVE
To prepare magnetoliposome (MLP) containing dextran-encapsulated magnetite (Fe3O4), and to examine its physicochemical properties and its in vivo behavior on ICR mice.


METHODS
Reverse phase evaporation method was used to formulate MLP and the Fe2+ concentration was measured by o-phenanthroline method. Then the basic properties of MLP and in vivo distribution were studied with the aid of 3H isotope as biomarker.


RESULTS
The mean diameter of MLP was 602.5 nm and the final concentration of encapsulated Fe3O4 was 88.1 mg x L(-1). Under natural conditions most of the MLP was taken up by spleen after the administration via tail vain, but its uptake was reduced under the magnetic field. There was a great difference in vivo distribution between the left and right lobes of the liver and the left and right kidneys in magnetic fields.


CONCLUSIONS
Reverse phase evaporation method was utilized to prepare magnetoliposomes. The formulation was stable and encapsulated high amount of magnetite. The delivery system could be oriented to certain tissues under magnetic field and satisfying magnetic responsiveness was observed.
Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Kuang-shi
KS
Wu
Jin-tian
JT
Tang
Xuan
X
Liu
Qiang
Q
Zhang
D000818:Animals; D003911:Dextrans / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005260:Female; D052203:Ferrosoferric Oxide; D007501:Iron / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007668:Kidney / Q000378:metabolism*; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D008280:Magnetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D036103:Nanotechnology; D010087:Oxides / Q000008:administration & dosage* / Q000493:pharmacokinetics; D010316:Particle Size; D013154:Spleen / Q000378:metabolism
D003911:Dextrans; D008081:Liposomes; D010087:Oxides; D007501:Iron; D052203:Ferrosoferric Oxide