Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria.
The chemical compositions of the essential oil of Curcuma zedoaria (Berg.) Rosc. were analyzed by gas chromatography-mass spectrometry (GC-MS) and showed a high content of epicurzerenone and curdione representing 46.6% and 13.7% of the total oil, respectively. The essential oil was evaluated for potential antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonasa aeruginosa, Vibrio parahaemolyticus, Salmonella typhimurium and Bacillus cereus. V. parahaemolyticus was sensitive to the presence of the essential oil, while the most resistant strain appeared to be E. coli. Based on 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay, nitroblue tetrazolium (NBT) reduction and cell morphology, the essential oil of C. zedoaria could inhibit the proliferation of human promyelocytic leukemia HL-60 cells. These results suggest that the essential oil has the antimicrobial activity against some of Gram- positive and negative pathogenic microorganisms and the components of the extract lead to the apoptosis of human cancer cell line.
Department of Nursing, Hungkuang University, Taichung 433-2, Taiwan.
Eric Y C
EY
Lai
Charng-Cherng
CC
Chyau
Jeng-Leun
JL
Mau
Chien-Chou
CC
Chen
Yi-Jui
YJ
Lai
Ching-Fang
CF
Shih
Long-Liu
LL
Lin
D017209:Apoptosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy; D018922:HL-60 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*
D009822:Oils, Volatile