IL-4 deficiency does not impair the ability of dendritic cells to initiate CD4+ and CD8+ T cell responses in vivo.
Several reports have described a role of IL-4 in dendritic cell function. We have examined the number and phenotype of dendritic cells from C57Bl/6 wild-type and IL-4-/- mice, and compared their ability to induce T cell immune responses in vivo and in vitro. We observed that the number of dendritic cells in the spleens and lymph nodes of IL-4-/- mice is comparable to the number found in wild-type mice. In addition, the expression of maturation markers such as MHC II, CD40, CD80 and CD86, and of differentiation markers such as CD4, CD8 and CD11b, was also comparable in the two populations. Splenic wild-type and IL-4-/- dendritic cells were both able to present antigen to T cell receptor transgenic CD4+ or CD8+ T cells in culture. When pulsed with antigen in vitro and then injected subcutaneously into C57BL/6 host mice, both populations of dendritic cells were able to induce the division of T cell receptor transgenic CD4+ or CD8+ T cells in vivo. This was the case regardless of whether the antigen used in these experiments was a low or a high affinity T cell receptor ligand. Similarly, both populations of dendritic cells were able to activate antigen-specific cytotoxic T cell responses and initiate tumor-protective immune responses in vivo. We conclude that IL-4-/- and wild-type dendritic cells have a comparable ability to initiate T cell immune responses when in an IL-4-sufficient environment.
Malaghan Institute of Medical Research, Wellington, New Zealand.
Joanna M
JM
Roberts
Jianping
J
Yang
Franca
F
Ronchese
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005434:Flow Cytometry; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000172:deficiency*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology
D015847:Interleukin-4