[Effect of dexamethasone on adhesion and phagocytosis of bovine trebacular meshwork cells].
OBJECTIVE
To study the effect of dexamethasone on adhesion and phagocyosis of bovine trebacular cells (BTC).


METHODS
After treated with different concentrations of dexamethasone (10(-7) M, 10(-6) M, 10(-5) M) for three days, BTC were added to 96-well plates coated with various extracellular matrix (ECM) and incubated for 90 minutes at 37 degrees C, Methyl thiazolyl tetrazolium (MTT) assay were used to evaluate the amount of the cells attach to ECM; In addition, using latex micropheries 0.8 microm in diameter as markers,we investigated the effect of dexamethasone on phagocytosis of BTC.


RESULTS
All groups of Dexamethasone can inhibit BTC attach to various ECM and in a dose-depend manner; the phagocytosis of BTC were also inhibited by three concentrations of dexamethasone and in a dose-depend manner.


CONCLUSIONS
Dexamethasone can inhibit adhesion and phagocytosis of BTC, it may be related with pathogenasis of glucocorticoid induced glaucoma.
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo
Z
Li
Hong
H
Zhang
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D014129:Trabecular Meshwork / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
D007166:Immunosuppressive Agents; D003907:Dexamethasone