Downmodulation of CD3epsilon expression in CD8alpha+beta- T cells of feline immunodeficiency virus-infected cats.
Feline immunodeficiency virus (FIV) infection in cats is associated with an increase of feline CD (fCD)8alpha+beta- and fCD8alpha+beta low cells in peripheral blood. To investigate these cells in more detail, an anti-fCD3epsilon mAb, termed NZM1, was generated, which recognizes the extracellular epitope of the fCD3epsilon molecule. The anti-fCD3epsilon mAb proved to be more suitable for identifying feline T cells than the anti-fCD5 one, which has been used as a pan-T-cell reagent in cats, because of the presence of fCD5+fCD3epsilon- cells among lymphocytes. Although the fCD8alpha+beta- and fCD8alpha+beta low cells in the FIV-infected cats expressed fCD3epsilon, a subset of fCD8alpha+beta- cells expressed fCD3epsilon antigen at a lower level than the T cells whose phenotype was fCD4+, or fCD8alpha+beta low. The lower expression of fCD3epsilon may be associated with the immune status of fCD8alpha+beta- T cells.
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yorihiro
Y
Nishimura
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Masayuki
M
Shimojima
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Eiji
E
Sato
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoshihiro
Y
Izumiya
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yukinobu
Y
Tohya
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Takeshi
T
Mikami
Laboratory of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.|Host and Defense, PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.|Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Takayuki
T
Miyazawa
D000818:Animals; D017252:CD3 Complex / Q000032:analysis* / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002415:Cats; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015536:Down-Regulation; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology*; D005434:Flow Cytometry; D016089:Immunodeficiency Virus, Feline; D007963:Leukocytes, Mononuclear; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
D017252:CD3 Complex; D016693:Receptors, Antigen, T-Cell, alpha-beta