Ascorbic acid responsive genes during neuronal differentiation of embryonic stem cells.
Ascorbic acid has been reported to enhance differentiation of embryonic stem (ES) cells into neurons, however, the specific functions of ascorbic acid have not been defined yet. To address this issue, gene expression profiling was performed using cDNA microarray. Ascorbic acid increased the expressions of genes involved in neurogenesis, maturation, and neurotransmission. Furthermore, statistical analysis using Fisher's exact test revealed ascorbic acid significantly modulated the genes involved in cell adhesion and development category. These results provide information on the role for ascorbic acid during neuronal differentiation of ES cells and might contribute to large-scale generation of neurons for future clinical treatment.
Shin
Dong-Mi
DM
Department of Food and Nutrition, Seoul National University, Seoul 151-742, Korea.
Ahn
Joon-Ik
JI
Lee
Ki-Hwan
KH
Lee
Yong-Sung
YS
Lee
Yeon-Sook
YS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers; D015152:Blotting, Northern / Q000379:methods; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012701:Serotonin / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology; D014404:Tubulin / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D012333:RNA, Messenger; D014404:Tubulin; C462941:beta3 tubulin, mouse; D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase; D001205:Ascorbic Acid