The fluorescence enhancement effect of the Tb-Gd-guanosine-5'-triphosphate-phen system and its analytical application.
It has been found that Tb(3+) can react with guanosine-5'-triphosphate (GTP) and o-phenanthroline (phen), resulting in the intrinsic fluorescence of Tb(3+). This fluorescence can be enhanced by adding La(3+), Gd(3+), Lu(3+), Sc(3+), Y(3+), and so on, among which Gd(3+) produces the greatest enhancement. These are new co-luminescence systems. The experiments indicate under optimum conditions, the fluorescence intensity of the Tb-Gd-GTP-phen system is proportional to the concentration of GTP over the range 1x10(-9) to 3x10(-5) mol/l. The detection limit is 3.1x10(-10) mol/l. The proposed method provides the most sensitive fluorimetry of GTP so far. The mechanism of the Tb-Gd-GTP-phen system has also been studied. The data indicates that there is a large congeries of Tb-Gd-GTP-phen, and the fluorescence enhancement of the Tb-Gd-GTP-phen system is considered to originate from intramolecular and intermocular energy transfers, and the energy-insulating sheath of the Gd complex.
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
Zongshan
Z
Zhao
Jinghe
J
Yang
Xia
X
Wu
Benyu
B
Su
Changxia
C
Sun
Shufang
S
Liu
D005682:Gadolinium / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013725:Terbium / Q000737:chemistry*
D009942:Organometallic Compounds; D010618:Phenanthrolines; D013725:Terbium; D006160:Guanosine Triphosphate; D005682:Gadolinium; C025205:1,10-phenanthroline