[Comparative characterization of molecular and microbiological methods for controlling chemotherapy in new cases of pulmonary tuberculosis].
The purpose of the study was to define a role of molecular genetic techniques in the evaluation of the efficacy of short-term chemotherapy in patients with pulmonary tuberculosis. A total of 203 patients with first detected tuberculosis patients who isolated Mycobacterium tuberculosis (MBT) were examined. Microbiological methods (bacterioscopy and seeding) and polymerase chain reaction (PCR) were used to detect MBT in new cases. During chemotherapy, there was a reduction in the number of positive results of both bacterioscopy and PCR, which serves as a sign of abacillation. Following 2 months of chemotherapy, 50% of the patients with negative bacterioscopy and seeding were found to have MBT DNA. In the patients with negative bacterioscopy and seeding, the results of PCR were also negative by month 6. In a group of patients without bacterial isolation, but with positive sputum tests for MBT DNA, the efficiency of chemotherapy was confirmed by the negative results of PCR. The latter is shown to enhance the sensitivity of bacteriological tests for MBT and to reduce the time of obtaining their results.
Larionova
E E
EE
Kuz'min
A V
AV
Vavil'eva
I A
IA
Chernousova
L N
LN
Golyshevskaia
V I
VI
D000995:Antitubercular Agents / Q000627:therapeutic use*; D001431:Bacteriological Techniques / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology
D000995:Antitubercular Agents