Inactivation of enteric microbes in water by electro-chemical oxidant from brine (NaCl) and free chlorine.
Oxidant solutions of mostly free chlorine can be electrochemically produced on-site from brine (NaCl) solution and used to disinfect water at the household or community level. In this study electrochemical oxidant (ECO) from brine and free chlorine were evaluated under laboratory conditions for inactivation of test microbes. Purified suspensions of Escherichia coli, the rugose strain of Vibrio cholerae, Clostridium perfringens spores, MS2 coliphage and Cryptosporidium parvum oocysts were treated with 2 mg/L or 5 mg/L solutions of ECO or free chlorine at 5 degrees C and 25 degrees C and pH 6, 8, and 10 (pH 7 and 25 degrees C only for C. parvum oocysts) for contact times <60 min. Under nearly all conditions, inactivation kinetics were more rapid for E. coli, V. cholerae, C. perfringens spores and MS2 coliphage with ECO than with free chlorine. ECO reduced E. coli, V. cholerae and MS2 by >4 log10 within 30 min and C. perfringens spores by >2 log10 within 10 min at pH 8 and 25 degrees C. Contrary to previous results, however, C. parvum oocysts were not inactivated by ECO, and the reasons for this difference are uncertain. The on-site electrolytic generation of oxidants from brine provided a convenient and inexpensive disinfectant containing free chlorine that was effective against many enteric microbes, for the treatment of household and community drinking-water supplies worldwide. However, the effectiveness of such oxidants for inactivating C. parvum oocysts was variable and sometimes ineffective.
Dept of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599-7431, USA.
L V
LV
Venczel
C A
CA
Likirdopulos
C E
CE
Robinson
M D
MD
Sobsey
D000818:Animals; D002713:Chlorine; D003016:Clostridium perfringens / Q000472:pathogenicity*; D016785:Cryptosporidium parvum / Q000472:pathogenicity*; D004203:Disinfection / Q000379:methods*; D004563:Electrochemistry; D004926:Escherichia coli / Q000472:pathogenicity*; D007700:Kinetics; D033661:Oocysts; D012492:Salts / Q000737:chemistry; D014734:Vibrio cholerae / Q000472:pathogenicity*; D018508:Water Purification / Q000379:methods*
D012492:Salts; C017082:brine; D002713:Chlorine