Expression and clinical significance of CD44V5 and CD44V6 in resectable colorectal cancer.
OBJECTIVE
This study was conducted to evaluate the prognostic significance of CD44v5 and CD44v6 in resectable colorectal cancer.


METHODS
Membranous CD44v5 and CD44v6 levels were measured by an immunoenzymatic assay in tumors and surrounding mucosal samples obtained from 105 patients with resectable colorectal carcinomas.


RESULTS
There were no significant differences of CD44v5 levels between tumors [median: 3.2 (range: 0.9-83.5) ng/mg protein) and surrounding mucosal samples (3 (3-146.2) ng/mg protein]. However, tumor samples showed significantly higher CD44v6 levels [19.5 (2.2-562.9) ng/mg protein] than mucosal samples [5 (5-230) ng/mg protein] (P=0.0001). Patients with higher CD44v5 or CD44v6 content in tumor samples had a considerably shorter relapse-free survival (P<0.05, for both). Patients with a higher CD44v6 content also had a shorter relapse-free and overall survival in the multivariate analysis (P<0.05).


CONCLUSIONS
The results of this study suggest a role of CD44v5 and CD44v6 in colorectal cancer progression. Membranous CD44v levels in primary tumors, measured by immunoenzymatic assay, may contribute to a more precise prognostic estimation in patients with resectable colorectal cancer.
Servicio de Cirugía General, Hospital de Jove, Avda. Eduardo Castro s/n, 33920, Gijón, Asturias, Spain, fjvizoso@wanadoo.es
Francisco J
FJ
Vizoso
José C
JC
Fernández
María D
MD
Corte
Miguel
M
Bongera
Richard
R
Gava
María T
MT
Allende
José L
JL
García-Muñiz
Manuel
M
García-Morán
D000368:Aged; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology / Q000601:surgery*; D002448:Cell Adhesion; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery*; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000276:immunology; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011237:Predictive Value of Tests; D011379:Prognosis; D011446:Prospective Studies; D012680:Sensitivity and Specificity
C113800:CD44v5 antigen; C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors