[Increased expression of heparanase mRNA is correlated with metastasis of gastric carcinoma and is probably correlated with c-met protein].
OBJECTIVE
To explore the expression of human heparanase mRNA and its relationship with the clinicopathological characteristics and angiogenesis and its possible mechanism in gastric carcinomas.


METHODS
Expression of heparanase mRNA in gastric cancerous tissues and its corresponding adjacent tissues were detected by RT-PCR in forty-seven patients. Expression of c-met protein and microvessel density (MVD) were examined by immunohistochemcal staining.


RESULTS
Expression of heparanse mRNA was significantly higher in gastric cancerous tissues than in its adjacent tissues (74.5% vs. 19.1%, P < 0.01). MVD was significantly higher in heparanase positive group than its counterpart (51 +/- 15 vs. 31 +/- 10, P < 0.01). Expression of heparanase mRNA was correlated with expression of c-met (r = 0.714, P < 0.01). The positive rate of heparanse expression in gastric carcinomas were correlated with tumor diameter, invasive depth, regional lymph nodes metastasis and TNM stage, whereas there were no relation with gender, age, tumor differentiation and remote metastasis of the patient.


CONCLUSIONS
Increased heparanase expression contributes to metastasis and angiogenesis in gastric cancer. Heparanase mRNA expression correlates with poor clinicopathologic characteristics in its prognosis. C-met is correlated with heparanase expression.
Cai
Yong-guo
YG
The Third Military South West Hospital, Repartarment of Gastrointes-tinal, Chongqing 400038, China.
Fang
Dian-chun
DC
Yang
Shi-ming
SM
Luo
Yuan-hui
YH
Yang
Meng-hua
MH
Wang
Dong-xu
DX
D000328:Adult; D000368:Aged; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit; D009389:Neovascularization, Pathologic; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D000099175:Heparanase
D005966:Glucuronidase; D052002:NF-kappa B p50 Subunit; D019859:Proto-Oncogene Proteins c-met; D012333:RNA, Messenger; D014157:Transcription Factors; D000099175:Heparanase; C489548:NFKB1 protein, human