Mechanism of CD150 (SLAM) down regulation from the host cell surface by measles virus hemagglutinin protein.
Measles virus has been reported to enter host cells via either of two cellular receptors, CD46 and CD150 (SLAM). CD46 is found on most cells of higher primates, while SLAM is expressed on activated B, T, and dendritic cells and is an important regulatory molecule of the immune system. Previous reports have shown that measles virus can down regulate expression of its two cellular receptors on the host cell surface during infection. In this study, the process of down regulation of SLAM by measles virus was investigated. We demonstrated that expression of the hemagglutinin (H) protein of measles virus was sufficient for down regulation. Our studies provided evidence that interactions between H and SLAM in the endoplasmic reticulum (ER) can promote the down regulation of SLAM but not CD46. In addition, we demonstrated that interactions between H and SLAM at the host cell surface can also contribute to SLAM down regulation. These results indicate that two mechanisms involving either intracellular interactions between H and SLAM in the ER or receptor-mediated binding to H at the surfaces of host cells can lead to the down regulation of SLAM during measles virus infection.
Welstead
G Grant
GG
Department of Medical Biophysics, University of Toronto, Canada.
Hsu
Eric C
EC
Iorio
Caterina
C
Bolotin
Shelly
S
Richardson
Christopher D
CD
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology / Q000821:virology; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000821:virology; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008459:Measles virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000502:physiology; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1
D015703:Antigens, CD; C497857:CD46 protein, human; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; C032397:hemagglutinin protein G, measles virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1