AID is required for c-myc/IgH chromosome translocations in vivo.
Chromosome translocations between c-myc and immunoglobulin (Ig) are associated with Burkitt's lymphoma in humans and with pristane- and IL6-induced plasmacytomas in mice. These translocations frequently involve Ig switch regions, suggesting that they might be the result of aberrant Ig class switch recombination (CSR). However, a direct link between CSR and chromosome translocations has not been established. We have examined c-myc/IgH translocations in IL6 transgenic mice that are mutant for activation induced cytidine deaminase (AID), the enzyme that initiates CSR. Here we report that AID is essential for the c-myc/IgH chromosome translocations induced by IL6.
Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA.
Almudena R
AR
Ramiro
Mila
M
Jankovic
Thomas
T
Eisenreich
Simone
S
Difilippantonio
Selina
S
Chen-Kiang
Masamichi
M
Muramatsu
Tasuku
T
Honjo
André
A
Nussenzweig
Michel C
MC
Nussenzweig
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002469:Cell Separation; D002875:Chromosomes / Q000648:ultrastructure; D003564:Cytidine Deaminase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D013997:Time Factors; D014178:Translocation, Genetic; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D007074:Immunoglobulin G; D007136:Immunoglobulins; D015850:Interleukin-6; D016271:Proto-Oncogene Proteins c-myc; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase