[Simultaneous determination of pseudoephedrine and chlorpheniramine in human plasma by HPLC-UV detection method].
OBJECTIVE
To establish a sensitive and specific method to simultaneous determination of pseudoephedrine and chlorpheniramine in human plasma.


METHODS
Pseudoephedrine and chlorpheniramine were extracted from alkaline plasma with t-butyl methyl ether as the base form, and were back-extracted into 1.5% hydrochloride solution. The two drugs were simultaneous determined by RP-HPLC with ultraviolet detection at 200 nm, using dextromethorphan as internal standard. A C18 column (250 mm x 46 mm ID) and a mobile phase containing acetonitrile-water-triethylamine (46:54:0.2, containing 10 mmol x L(-1) sodium dodecyl sulfate (SDS) and 60 mmol x L(-1) NaH2 PO4, adjusted pH to 2.6 with H3PO4) were used.


RESULTS
The limit of quantification was 10.0 and 0.5 microg x L(-1), the linear range was 1.5 - 0.01 mg x L(-1) and 75 - 0.5 microg x L(-1), for pseudoephedrine and chlorpheniramine, respectively. The within-day and between-day RSD were less than 12.4%, and the average recovery was between 97.3% - 109.4%.


CONCLUSIONS
The method was sensitive, specific, simple, and suitable for drug level monitoring in clinical pharmacokinetic study.
Ge
Qing-hua
QH
National Pharmaceutical Engineering Research Center, Shanghai 200437, China. geqinghua@sina.com
Zhou
Zhen
Z
Zhi
Xiao-jin
XJ
Wang
Hao
H
D000328:Adult; D002744:Chlorpheniramine / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003692:Delayed-Action Preparations; D004809:Ephedrine / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D013056:Spectrophotometry, Ultraviolet
D003692:Delayed-Action Preparations; D002744:Chlorpheniramine; D004809:Ephedrine