FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and FtsY. Although flhF is located in the fla/che operon, B. subtilis 168 flhF mutant cells assemble flagella and are motile.
Zanen
Geeske
G
Department of Molecular Bacteriology, University of Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.
Antelmann
Haike
H
Westers
Helga
H
Hecker
Michael
M
van Dijl
Jan Maarten
JM
Quax
Wim J
WJ
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D005407:Flagella / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D009068:Movement; D016254:Mutagenesis, Insertional; D009154:Mutation; D009876:Operon; D021381:Protein Transport; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D018271:Signal Recognition Particle / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D020543:Proteome; D018271:Signal Recognition Particle; C078317:flhF protein, Bacteria; D020559:Monomeric GTP-Binding Proteins