[The comparison of three ways for extracting nucleic acid from rats hair].
OBJECTIVE
To discuss the method of extracting nucleic acid from rat's hair.


METHODS
The method with PCR buffer, SDS-proteinase K or Chelex-100 were used to extract the nucleic acid from rat's hair separately, and the isolated nucleic acid was analyzed by PCR and electrophoresis.


RESULTS
The success rate of antracting mt DNA from the hair gollcle, we gound the method with SDS-prot einase kis lower than the ather two methods (chi1(2) = 42.421, chi2(2) = 28.800, P<0.01). This is same as the result ot entracting nuclear DNA (chi1(2) = 49.091, chi2(2) = 30.767, P<0.01). While no alifference has been bound between the method with PCR buffer and with chelex-100 (mtDNA: chi(2) = 0.296; nuclear DNA: chi(2) = 0.048, P>0.05). The method with PCR buffer can't extract nucleic acid from hair shafts, the method with SDS-proteinase K can't extract mtDNA from one or two hairs, the method with Chelex-100 can extract mtDNA and nuclear DNA from single rat's hair or hair shafts.


CONCLUSIONS
The method with Chelex-100 is suitable for extracting nucleic acid from hair.
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Yujuan
Y
Hu
Weijia
W
Kong
Yuechen
Y
Han
Ying
Y
Wang
Huamao
H
Cheng
Min
M
Chen
D000818:Animals; D002614:Chelating Agents; D004247:DNA / Q000235:genetics; D006197:Hair / Q000737:chemistry*; D009696:Nucleic Acids / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D051381:Rats; D012117:Resins, Synthetic
D002614:Chelating Agents; D009696:Nucleic Acids; D012117:Resins, Synthetic; C024997:Chelex 100; D004247:DNA