Gene expression in mononuclear cells from patients with inflammatory bowel disease.
OBJECTIVE
Discovery of Nod2 as the inflammatory bowel disease 1 (IBD1) susceptibility gene has brought to light the significance of mononuclear cells in inflammatory bowel disease pathogenesis. The purpose of this study was to examine changes in gene expression in peripheral blood mononuclear cells in patients with untreated Crohn's disease (CD) and ulcerative colitis (UC) as compared to patients with other inflammatory gastrointestinal disorders and to healthy controls.


METHODS
We used a 2400 gene cDNA glass slide array (MICROMAX) to examine gene expression in peripheral blood mononuclear cells from seven patients with Crohn's disease, five patients with ulcerative colitis, 10 patients with other inflammatory gastrointestinal disorders, and 22 age- and sex-matched controls. Results. Novel categories of genes differentially expressed in Crohn's disease and ulcerative colitis patients included genes regulating hematopoietic cell differentiation and leukemogenesis, lipid raft-associated signaling, the actin cytoskeleton, and vesicular trafficking.


CONCLUSIONS
Altered gene expression in mononuclear cells may contribute to inflammatory bowel disease pathogenesis.
Stanley S. Scott Cancer Center, New Orleans, LA 70112, USA. emanni@lsuhsc.edu
Elizabeth E
EE
Mannick
Joseph C
JC
Bonomolo
Ronald
R
Horswell
Jennifer J
JJ
Lentz
Maria-Stella
MS
Serrano
Adriana
A
Zapata-Velandia
Mariella
M
Gastanaduy
Jessica L
JL
Himel
Steven L
SL
Rose
John N
JN
Udall
Conrad A
CA
Hornick
Zhiyun
Z
Liu
D003093:Colitis, Ulcerative / Q000097:blood* / Q000235:genetics*; D003424:Crohn Disease / Q000097:blood* / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger