Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues.
The goal of this study was to generate cell lines from a variety of insect tissues that could be useful for developing in vitro assays with tissue-specific properties. In this article, we describe the establishment of new cell cultures from differentiated (primarily neural) and undifferentiated tissues (primarily embryonic) and their initial characterization. Cell lines were established from the following tissues of the budworm, Heliothis virescens, and the bollworm, Helicoverpa zea: larval ventral nerve cords (4 lines), larval midguts (1 line), adult ovaries (1 line), and embryonic tissues (11 lines). Cell lines were primarily characterized by morphological examination and polymerase chain reaction (PCR) (both deoxyribonucleic acid amplification fingerprinting and inter-simple sequence repeats PCR).
Biological Control of Insects Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Columbia, Missouri 65203-3535, USA. goodmanc@missouri.edu
Cynthia L
CL
Goodman
Amy A
AA
Wang
Henda
H
Nabli
Arthur H
AH
McIntosh
Jennifer L
JL
Wittmeyer
James J
JJ
Grasela
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting; D005260:Female; D017344:Genes, Insect; D007422:Intestines / Q000196:embryology; D007814:Larva / Q000166:cytology; D009036:Moths / Q000166:cytology* / Q000235:genetics; D009474:Neurons / Q000166:cytology; D010053:Ovary / Q000166:cytology; D016133:Polymerase Chain Reaction
D004247:DNA