A field comparison of four fungal aerosol sampling instruments: inter-sampler calibrations and caveats.
Four bioaerosol samplers (Reuter Centrifugal, Andersen N6 Single Stage, Surface Air System Super 90, and Air-o-Cell) were used to take c. 300 side-by-side measurements at 75 public building sites. Regression models were developed to examine the relationships between each method pair. The models demonstrate that measurements from these instruments are not directly comparable, requiring inter-instrument calibration. Sampling location (indoor vs. outdoor) was a confounder in all the pairwise comparisons between samplers. In addition, the slopes of the relationships between all method pairs except one differed in indoor vs. outdoor locations. These results emphasize that direct comparisons between methods should not be undergone without prior calibration. Where measurement circumstances are similar to those of this study, the regression models might serve as a basis to convert measurements made with one instrument to those made with another. However, the robustness and generalizability of the models in different measurement settings needs to be assessed.


CONCLUSIONS
Many different bioaerosol sampling devices are in common use for indoor air quality studies. If data from research studies are to be compared, an approximation of the relationships between the equipment would be useful. A comparison of three culturable sampling devices (Andersen N6, SAS 90, RCS) and one particulate sampling device (Air-o-Cell) collecting simultaneous samples under field conditions showed high linear correlations between methods. However, while direct comparisons between sampling data were not possible, the regression models reported here explained 60-85% of the variance in fungal concentrations, and underscored the importance of the effect of environment on measurement.
School of Occupational and Environmental Hygiene, University of British Columbia, Vancouver, BC, Canada.
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Lee
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Teschke
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Brauer
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Bartlett
D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D002138:Calibration; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D005658:Fungi / Q000302:isolation & purification*; D012044:Regression Analysis