In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites.
Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (beta-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 microg/mL) and beta-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites).
Department of Biomaterials & Biomimetics, New York University College of Dentistry, 345 E. 24th Street New York, New York 10010, USA. mi8@md.okayama-u.ac.jp
Miho
M
Inoue
Racquel Z
RZ
LeGeros
Masahisa
M
Inoue
Hidetsugu
H
Tsujigiwa
Hitoshi
H
Nagatsuka
Toshio
T
Yamamoto
Noriyuki
N
Nagai
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001031:Apatites / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009804:Odontoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009928:Organ Specificity; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared
D001031:Apatites; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase