Requirement of Rac1 and Rac2 expression by mature dendritic cells for T cell priming.
Upon maturation, dendritic cells (DCs) acquire the unique ability to activate naïve T cells. We used time-lapse video microscopy and two-photon imaging of intact lymph nodes to show that after establishing initial contact between their dendrites and naïve T lymphocytes, mature DCs migrate toward the contacted lymphocytes. Subsequently, the DCs tightly entrap the T cells within a complex net of membrane extensions. The Rho family guanosine triphosphatases Rac1 and Rac2 but not Rho itself control the formation of dendrites in mature DCs, their polarized short-range migration toward T cells, and T cell priming.
Benvenuti
Federica
F
Unité INSERM 365, Institut Curie, 26 rue d'Ulm, 75005 Paris, France.
Hugues
Stephanie
S
Walmsley
Marita
M
Ruf
Sandra
S
Fetler
Luc
L
Popoff
Michel
M
Tybulewicz
Victor L J
VL
Amigorena
Sebastian
S
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002450:Cell Communication; D016764:Cell Polarity; D022081:Cell Surface Extensions / Q000502:physiology / Q000648:ultrastructure; D003599:Cytoskeleton / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018715:Microscopy, Video; D020744:rac GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D000097766:RAC2 GTP-Binding Protein
D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein; D020741:rho GTP-Binding Proteins; D000097766:RAC2 GTP-Binding Protein