Serum/Plasma depletion with chicken immunoglobulin Y antibodies for proteomic analysis from multiple Mammalian species.
Plasma from different species is the most accessible and valuable source for biomarker discovery in clinical and animal samples. However, due to the high abundance of some proteins such as albumin and immunoglobulins, low-abundant proteins are often undetectable in proteomic analysis of plasma. We have established a plasma depletion scheme using chicken antibodies against various abundant proteins. This immunoaffinity purification procedure is able to deplete albumin across multiple species. The high binding capacity and specificity of the chicken antibody enables the efficient capture of its ligand from microliter volumes of plasma sample. The resulting two-dimensional gel analyses of the depleted and captured samples show significant enhancement of the low-abundant proteins and specific capture of the abundant ligand. By utilizing this sample preparation scheme, it is now possible to analyze the plasma proteome from multiple species in a potentially rapid and large-scale capacity for biomarker discovery, drug target discovery, and toxicology studies.
Hinerfeld
Douglas
D
Charles River Proteomic Services, 57 Union Street, Worcester, MA 01608, USA.
Innamorati
David
D
Pirro
John
J
Tam
Sun W
SW
D000818:Animals; D000906:Antibodies / Q000097:blood*; D002645:Chickens / Q000276:immunology*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D008863:Microspheres; D051381:Rats; D012709:Serum Albumin / Q000032:analysis / Q000302:isolation & purification*
D000906:Antibodies; C016512:IgY; D007136:Immunoglobulins; D012709:Serum Albumin