Characterization of a CENP-C homolog in Arabidopsis thaliana.
Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.
Ogura
Yutaka
Y
Research Institute for Bioresources, Okayama University, Kurashiki, Japan. yogura@rib.okayama-u.ac.jp
Shibata
Fukashi
F
Sato
Hiroshi
H
Murata
Minoru
M
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D002503:Centromere / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; C060905:centromere protein C