Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus.
To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arrays. Caco-2 and CL-14 cell lines were found to be highly permissive to SARS-CoV, due to the presence of angiotensin-converting enzyme 2 as a functional receptor. In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS.
Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul-Ehrlich Str. 40, 60596, Frankfurt am Main, Germany. Cinatl@em.uni-frankfurt.de
J
J
Cinatl
G
G
Hoever
B
B
Morgenstern
W
W
Preiser
J-U
JU
Vogel
W-K
WK
Hofmann
G
G
Bauer
M
M
Michaelis
H F
HF
Rabenau
H W
HW
Doerr
D018938:Caco-2 Cells; D002470:Cell Survival; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000648:ultrastructure / Q000821:virology*; D008854:Microscopy, Electron; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D011991:Receptors, Virus / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*
D011991:Receptors, Virus