Photo-acceleration of protein release from endosome in the protein transduction system.
The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.
Department of Physiology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. masayuki@cc.okayama-u.ac.jp
Masayuki
M
Matsushita
Hirofumi
H
Noguchi
Yun-Fei
YF
Lu
Kazuhito
K
Tomizawa
Hiroyuki
H
Michiue
Sheng-Tian
ST
Li
Kenzo
K
Hirose
Susan
S
Bonner-Weir
Hideki
H
Matsui
D004705:Endocytosis; D011992:Endosomes / Q000502:physiology* / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008027:Light; D009363:Neoplasm Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D021381:Protein Transport / Q000528:radiation effects
D009363:Neoplasm Proteins; D010455:Peptides