[Expression of inducible nitric oxide synthase mRNA in epithelial cell of nasal mucosa is upregulated through Toll-like receptor-4].
OBJECTIVE
To investigate the upregulation of inducible nitric oxide synthase (iNOS) in epithelial cell of nasal mucosa through Toll-like receptor-4 (TLR-4).


METHODS
Specimens from 30 patients of chronic sinusitis and 21 healthy adults were examined by in situ hybridization for TLR-4 and iNOS mRNA.


RESULTS
All 30 samples of chronic sinusitis showed a stronger expression of TLR-4 and iNOS than in controls (P<0.01). iNOS upregulated in nasal epithelium correlated with TLR-4 (r=0.435, P<0.01).


CONCLUSIONS
It suggested that TLR-4 may play a role in enhancing local host defence and inflammation via upregulating the expression of iNOS mRNA and the subsequent increased production of NO.
Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun, 130031, China.
Chengshuo
C
Wang
Zhen
Z
Dong
Guimei
G
Guan
Zhanquan
Z
Yang
D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D012852:Sinusitis / Q000378:metabolism; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II