Effect of Brazilian propolis on scratching behavior induced by compound 48/80 and histamine in mice.
We studied the effect of Brazilian propolis on scratching behavior induced by compound 48/80 and histamine in ICR mice. Propolis granular A.P.C dose-related inhibited scratching behavior induced by compound 48/80 and significant inhibition were observed at 1000 mg/kg. However, histamine-induced scratching behavior was not inhibited by propolis granular A.P.C even at 1000 mg/kg. Propolis ethanol extract at 10 microg/ml or more inhibited histamine release from rat mast cells induced by compound 48/80. In addition, it blocked increased vascular permeability induced by compound 48/80. The inhibitory effect of propolis on scratching behavior induced by compound 48/80 was gradually enhanced by repeated administration, and 500 mg/kg propolis granular A.P.C, which caused no effect through single administration, significantly inhibited scratching behavior after repeated administration for 4 weeks. From these findings, it is assumed that the inhibition of scratching behavior induced by propolis occurs through a mast cell-dependent mechanism.
Faculty of Pharmaceutical Sciences, Department of Pharmacology, Okayama University, Tushima-naka 1-1-1, Okayama 700-8530, Japan.
Yoshifumi
Y
Shinmei
Maria Alejandra
MA
Hossen
Kiyoshi
K
Okihara
Hiroyuki
H
Sugimoto
Hideo
H
Yamada
Chiaki
C
Kamei
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001938:Brazil; D002199:Capillary Permeability / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D006632:Histamine; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006636:Histamine Release / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011429:Propolis / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011537:Pruritus / Q000188:drug therapy* / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D003189:p-Methoxy-N-methylphenethylamine
D006634:Histamine H1 Antagonists; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D011429:Propolis