Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals.
Levels of antioxidant defenses and lipid peroxidation were evaluated in mussels exposed to lead (200 mg/l), iron (500 microg/l), cadmium (200 microg/l) and copper (40 microg/l), for 12, 24, 72 and 120 h. Glutathione S-transferase (GST) activity was unchanged with all treatments. Catalase (CAT) increased after 120 h of exposure to all metals. Mussels exposed to Cd for 12 h, and to Cu and Fe for 120 h had increased lipid peroxidation, which might be associated to decreased levels of reduced glutathione (GSH) and glutathione peroxidase (GPx) activity. Pb exposure caused GSH depletion after 12 h and increased GPx activity after 120 h. Negative correlations were observed between the enzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx) and malonaldehyde (MDA) levels after Fe and Cu exposure, indicating a protective role of PHGPx against lipid peroxidation, and suggesting the use of this enzyme as a new potential biomarker of toxicity associated with contaminant exposure in mussels.
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26.077, 05513-970 São Paulo, SP, Brazil.
Eduardo Alves
EA
de Almeida
Sayuri
S
Miyamoto
Afonso Celso Dias
AC
Bainy
Marisa Helena Gennari
MH
de Medeiros
Paolo
P
Di Mascio
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000502:physiology; D005979:Glutathione Peroxidase / Q000032:analysis / Q000494:pharmacology*; D015227:Lipid Peroxidation; D019216:Metals, Heavy / Q000633:toxicity*; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase
D000975:Antioxidants; D015415:Biomarkers; D019216:Metals, Heavy; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase