Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.
Cryocrystallography is routinely used in macromolecular crystallography laboratories. The main advantage of X-ray diffraction data collection near 100K is that crystals display much less radiation damage than seen at room temperature. Techniques and tools are described to facilitate cryoprotecting and flash-cooling crystals for data collection.
Pflugrath
J W
JW
Rigaku/MSC, Inc., 9009 New Trails Drive, The Woodlands, TX 77381, USA. jwp@RigakuMSC.com
D002623:Chemistry Techniques, Analytical; D003080:Cold Temperature; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry*
D011506:Proteins