Genotoxic and teratogenic potential of marine sediment extracts investigated with comet assay and zebrafish test.
Organic extracts of marine sediments from the North Sea and the Baltic Sea were investigated with two toxicity assays. The comet assay based on the fish cell line Epithelioma papulosum cyprini (EPC) was applied to determine the genotoxic potential; zebrafish embryos (Danio rerio) were used to quantify the teratogenic potential of the samples. EC(50) values were calculated from dose-response curves for both test systems. Highest teratogenic and genotoxic effects normalised to total organic carbon (TOC) content were detected in sediment samples of different origins. Polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) are not likely to be the causes of the observed effects, as demonstrated by a two-step fractionation procedure of selected extracts. The toxic potential was more pronounced in fractions having polarity higher than those possessed by PAHs and PCBs. The suitability of the two in vitro test systems for assessing genotoxic and teratogenic effects of marine sediment extracts could be demonstrated.
Kammann
Ulrike
U
Federal Research Centre for Fisheries, Institute for Fishery Ecology, Palmaille 9, 22767 Hamburg, Germany. ulrike.kammann@ifo.bfa-fisch.de
Biselli
Scarlett
S
Hühnerfuss
Heinrich
H
Reineke
Ninja
N
Theobald
Norbert
N
Vobach
Michael
M
Wosniok
Werner
W
D000818:Animals; D020552:Comet Assay; D004784:Environmental Monitoring / Q000379:methods*; D004787:Environmental Pollution; D019015:Geologic Sediments; D007928:Lethal Dose 50; D009152:Mutagenicity Tests; D012623:Seawater; D018675:Toxicity Tests; D015027:Zebrafish