Pro-oxidative effects of Tempo in systems containing oxidants.
2,2,6,6-Tetramethylpiperidine-1-oxyl (Tempo), previously reported by us to augment oxidation of glutathione induced by peroxynitrite (Glebska J, Skolimowski J, Kudzin Z, Gwozdzinski K, Grzelak A, Bartosz G. Pro-oxidative activity of nitroxides in their reactions with glutathione. Free Radic Biol Med 2003; 35: 310-316) was found to increase oxidation of glutathione induced by various oxidants, including persulfate, tert-butyl hydroperoxide and hydrogen peroxide. Tempo augmented also the inactivation and thiol loss of alcohol dehydrogenase induced by 2,2'-azobis(2-amidinopropane) (AAPH) and oxidative degradation of deoxyribose induced by ammonium persulfate and tert-butyl hydroperoxide. These results point to a pro-oxidative effect of nitroxides on a range of biomolecules subjected to the action of various oxidants.
Department of Molecular Biophysics, University of Lodz, Lodz, Poland. abal@biol.uni.lodz.pl
Aneta
A
Balcerczyk
Agnieszka
A
Grzelak
Miroslaw
M
Soszynski
Grzegorz
G
Bartosz
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000378:metabolism*; D003855:Deoxyribose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D010084:Oxidation-Reduction
D000975:Antioxidants; D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; D016877:Oxidants; D003855:Deoxyribose; D000426:Alcohol Dehydrogenase; D005978:Glutathione; C039900:nitroxyl; C003959:TEMPO