Association between the PPARalpha-L162V polymorphism and components of the metabolic syndrome.
Genetic factors, alone or in interaction with components of the diet, are thought to be involved in the development of the metabolic syndrome. The objective of our study was first to compare the frequency of the peroxisome proliferator-activated receptor (PPAR)alpha-L162V polymorphism in a sample of men with and without the metabolic syndrome as defined by the National Cholesterol Education Program-Adult Treatment Panel III (NCEP-ATPIII) guidelines, and secondly, to evaluate gene-diet interaction effects on features of the metabolic syndrome. The PPARalpha-L162V genotype was determined in a sample of 632 men by a polymerase chain reaction-restriction length polymorphism (PCR-RFLP)-based method; fat as well as saturated fat intakes were evaluated by a dietitian-administered food frequency questionnaire. The frequency of the V162 allele was similar in men with ( n=281) and without ( n=351) the metabolic syndrome ( chi(2)=0.03, p=0.84) but was higher in subjects having simultaneously abdominal obesity, hypertriglyceridemia, and low high-density lipoprotein cholesterol (HDL-C) levels ( chi(2)=3.73, p=0.05). Carriers of the V162 were characterized by higher plasma apolipoprotein B and triglyceride (TG) levels ( p=0.10, p=0.004). In a model including the PPARalpha-L162V polymorphism, fat or saturated fat, its interaction, and covariates (smoking habits, and energy and alcohol intake), the interaction explained a significant percentage of the variance observed in waist circumference ( p<0.05). In conclusion, the PPARalpha-L162V polymorphism alone or in interaction with dietary fat intake is associated with components of the metabolic syndrome.
Lipid Research Center, CHUQ-CHUL Pavilion, 2705 Laurier Blvd, TR-93, Ste-Foy, QC, Canada, G1V 4G2.|Food Science and Nutrition Department, Laval University, Ste-Foy, QC, Canada.
Julie
J
Robitaille
Lipid Research Center, CHUQ-CHUL Pavilion, 2705 Laurier Blvd, TR-93, Ste-Foy, QC, Canada, G1V 4G2.|Department of Social and Preventive Medicine, Division of Kinesiology, Laval University, Ste-Foy, QC, Canada.
Charles
C
Brouillette
Lipid Research Center, CHUQ-CHUL Pavilion, 2705 Laurier Blvd, TR-93, Ste-Foy, QC, Canada, G1V 4G2.
Alain
A
Houde
Food Science and Nutrition Department, Laval University, Ste-Foy, QC, Canada.
Simone
S
Lemieux
Department of Social and Preventive Medicine, Division of Kinesiology, Laval University, Ste-Foy, QC, Canada.
Louis
L
Pérusse
Lipid Research Center, CHUQ-CHUL Pavilion, 2705 Laurier Blvd, TR-93, Ste-Foy, QC, Canada, G1V 4G2.|Food Science and Nutrition Department, Laval University, Ste-Foy, QC, Canada.|Molecular Endocrinology and Oncology Research Center, CHUQ-CHUL Pavilion, Ste-Foy, QC, Canada.
André
A
Tchernof
Community Genomic Medicine Center, Montreal University and Lipid Clinic, Chicoutimi Hospital, Chicoutimi, QC, Canada.
Daniel
D
Gaudet
Lipid Research Center, CHUQ-CHUL Pavilion, 2705 Laurier Blvd, TR-93, Ste-Foy, QC, Canada, G1V 4G2. marie-claude.vohl@crchul.ulaval.ca.|Food Science and Nutrition Department, Laval University, Ste-Foy, QC, Canada. marie-claude.vohl@crchul.ulaval.ca.
Marie-Claude
MC
Vohl
D000328:Adult; D000428:Alcohol Drinking; D001055:Apolipoproteins B / Q000097:blood; D001837:Body Weights and Measures; D008078:Cholesterol, LDL / Q000097:blood / Q000235:genetics*; D004041:Dietary Fats; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000235:genetics*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011792:Quebec; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D012907:Smoking; D011795:Surveys and Questionnaires; D013577:Syndrome; D014157:Transcription Factors / Q000235:genetics*
D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D004041:Dietary Fats; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors