[Biodegradation of aromatic hydrocarbons and dynamics of microbe growth in soils contaminated with mineral oil].
Some bacteria and fungi selected from brown soil contaminated with petroleum were taken as test microbes. Degradation of mineral oil, by different combinations of microbes and the correlation between the degradation rate and microbial growth were studied. The bacteria and fungi were inoculated to a liquid substrate spiked with 1000 milligrams diesel-oil per liter. The temperature of rocking-bed was controlled at 25 to 30 centigrade and the experiment continued for 100 days. From 0 to 60 hours it was sampled continuously to monitor the dynamic of microbial growth, and sampled termly from 5 to 100 days to study the growth of microbes and the dynamic of degradation rate. At the same time, the suspension of 0 to 20 centimeters of topsoil of the meadow umber was taken as soil microbial control, and the culture without microbes as blank control. Results showed that degradation effect of the introduced microbes were superior to that of the indigenous microbes in short term (about 20 days), while the advantage of indigenous microbes got stronger and stronger, and the predominance maintained until the experiment was stopped. At the end of the experiment the degradation rate in the treatment of indigenous microbes reached 79.24%, which was above the results of other 3 treatments, and the difference was significant.
Song
Xue-ying
XY
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Song
Yu-fang
YF
Sun
Tie-heng
TH
Zhou
Qi-xing
QX
D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D006841:Hydrocarbons, Aromatic / Q000032:analysis / Q000378:metabolism*; D008899:Mineral Oil / Q000032:analysis / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D006841:Hydrocarbons, Aromatic; D012989:Soil Pollutants; D008899:Mineral Oil