Segmental isotopic labeling for structural biological applications of NMR.
This chapter describes the preparation of precursor domains for the formation of multidomain segmentally labeled proteins by protein ligation.
Cowburn
David
D
New York Structural Biology Center, New York, NY, USA.
Shekhtman
Alexander
A
Xu
Rong
R
Ottesen
Jennifer J
JJ
Muir
Tom W
TW
D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D015202:Protein Engineering; D011522:Protons; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D011522:Protons; D011993:Recombinant Fusion Proteins; D003545:Cysteine