Investigation of the use of immobilised metal affinity chromatography for the on-line sample clean up and pre-concentration of nucleotides prior to their determination by ion pair liquid chromatography-electrospray mass spectrometry: a pilot study.
This study explored an alternative way to enrich and pre-purify biological samples containing nucleoside mono-, di- and triphosphates. These compounds were trapped by immobilised metal affinity chromatography (IMAC) on a Poros 20 MC IMAC-column, which was conditioned with Fe3+. The IMAC-column was implemented in a column switching set-up separating nucleoside mono-, di- and triphosphates on a Hypersil ODS 35 mm x 0.3 mm capillary column hyphenated to electrospray mass spectrometry resulting in the first miniaturised column switching liquid chromatography-mass spectrometry (LC-MS) system for nucleotides.
The Nucleoside Research and Mass Spectrometry Unit, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Robin
R
Tuytten
Filip
F
Lemière
Walter
W
Van Dongen
Herman
H
Slegers
Russell P
RP
Newton
Eddy L
EL
Esmans
D002846:Chromatography, Affinity / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D009711:Nucleotides / Q000032:analysis / Q000302:isolation & purification*; D010865:Pilot Projects; D012680:Sensitivity and Specificity
D005290:Ferric Compounds; D009711:Nucleotides