[MN/CAIX gene expression in renal cell carcinoma detected by RT-PCR].
OBJECTIVE
To evaluate the reliability of reverse transcriptional (RT)-PCR in detecting MN/CAIX gene expression in renal cell carcinoma (RCC).


METHODS
MN/CAIX gene expression was detected in 5 RCC tissues and 5 normal renal tissues. The primers of MN/CAIX gene were designed for a two-step RT-PCR, and the products examined by 2% agarose gel electrophoresis and identified by sequence analysis.


RESULTS
Corresponding bands of MN/CAIX gene could be seen in RT-PCR products of cases RCC tissue from 4 but not in normal renal tissues.


CONCLUSIONS
RT-PCR is reliable for detecting the expression of MN/CAIX gene in RCC.
Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Yao-dong
YD
Jiang
Shao-bin
SB
Zheng
Zhan-hui
ZH
Wang
D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000096:biosynthesis* / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases