Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP.
Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.
Department of Cytogenetics and Plant Molecular Biology, University of Lodz, ul. Banacha 12/16, 90-237, Poland. beatakosmider@interia.pl
Beata
B
Kosmider
Elzbieta
E
Zyner
Regina
R
Osiecka
Justyn
J
Ochocki
D000165:Acridine Orange / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D002945:Cisplatin / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D004996:Ethidium / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling; D015394:Molecular Structure; D009336:Necrosis; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
C488624:3-aminoflavone; D000970:Antineoplastic Agents; D005419:Flavonoids; D005456:Fluorescent Dyes; D013778:Tetrazolium Salts; D013844:Thiazoles; D004996:Ethidium; C022616:thiazolyl blue; D000165:Acridine Orange; D002945:Cisplatin