A study on the activity of fibroblast cells in connection with tissue recovery in the wounds of skin injury after whole-body irradiation.
The 6 Gy of whole-body irradiation (WBI) with gamma rays results in an impairment of injured skin tissue recovery and renders a delay in the healing process. For an understanding of whether WBI has damaging effects on fibroblasts in wounds, fibroblasts in wounds combined with WBI and those of simple incision were isolated and cultivated, and abilities connected with tissue repair, including proliferation, attachment, adhesion, and apoptosis, were determined by direct cell count, immunohistochemical staining for proliferation cell nuclear antigen (PCNA), and TUNEL assay. The results showed that the abilities of proliferation and the attachment and adhesion of fibroblasts from wounds combined with WBI significantly decreased in comparison with those having simple incisions on the 3rd and 5th days of posttrauma, whereas the apoptotic ratio of fibroblasts from wounds combined with WBI significantly increased. These data suggest that WBI may exert damaging effects on fibroblasts in wounds, which might be one of the dominant reasons for the impaired healing of wounds combined with WBI.
Institute of Combined Injury of PLA, State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing, P.R. China.
Jifu
J
Qu
Tianmin
T
Cheng
Chunmeng
C
Shi
Yuan
Y
Lin
Xinze
X
Ran
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000473:pathology* / Q000528:radiation effects*; D005720:Gamma Rays; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000528:radiation effects*; D012867:Skin / Q000293:injuries* / Q000473:pathology / Q000528:radiation effects*; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000528:radiation effects*; D014950:Wounds, Penetrating / Q000473:pathology*