The use of the cMyc epitope tag can be problematic for protein detection in Legionella pneumophila.
In various recent experiments with respect to protein secretion by L. pneumophila, protein detection based on the cMyc-tag seemed to be problematic. For one specific protein, we studied the reason for this problem in more detail by comparing protein expression in the presence and absence of the cMyc-tag. We demonstrated a decrease in the amount of mRNA transcript when the cMyc coding sequence is present. We can conclude that this epitope tag is not a preferential tag for protein detection in L. pneumophila.
Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, Leuven B-3000, Belgium.
Emmy
E
De Buck
Ilya
I
Lebeau
Lieve
L
Van Mellaert
Nick
N
Geukens
Jozef
J
Anné
Elke
E
Lammertyn
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D000939:Epitopes; D016271:Proto-Oncogene Proteins c-myc; D012329:RNA, Bacterial