Phage display selection of scFv to murine endothelial cell membranes.
The diversity of endothelial cells is becoming more apparent and more important in defining vessel systems that supply blood to normal organs and to tumors. Reagents that identify expression of cell surface determinants on these cells are crucial for differentiating among different vessel types. As a first step in this process we have selected a panel of 25 scFvs from a phage display library that bind to the endothelial cell line LEII. The scFvs are of high affinity and bind to some tumor cells as well as to the target endothelial cell. The scFvs can be divided into 8 epitope groups by use of competition binding studies. DNA sequencing of the members of these groups generally support the classification. This work shows that phage display is a rapid and efficient method for identification of reagents for cell surface molecules.
Kennel
Stephen J
SJ
Life Sciences Division, Oak Ridge National Laboratory, TN 37831-6123, USA. kennelsj@ornl.gov
Lankford
Trish
T
Foote
Linda
L
Wall
Melissa
M
Davern
Sandra
S
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D051379:Mice
D000906:Antibodies; D017931:DNA Primers; D000939:Epitopes