[Construction of plasmid with green fluorescent protein gene for analysis of yeasts in yeast wastewater treatment].
Green fluorescent protein (GFP) can be utilized in analysis of the characteristics and distribution of a targeted strain in microbial communities. This study is the first step to establish a dynamic yeast monitoring technique in a wastewater treatment system using yeast by constructing a fluorescent yeast containing gfp gene. The gfp gene was inserted into pACT1-URA3, a powerful plasmid for introducing a foreign gene into Candida boidinii, and then transformed into E. coli JM109. The gfp gene was expressed, though not very highly. The results of the electrophoresis and polymerase chain reaction suggested that the newly constructed plasmid containing gfp gene might not exist in free form in the cells, but in some special way such as interaction with the chromosome.
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, CAS, Beijing 100085, China.
Yun
Y
Han
Xin-hui
XH
Xing
Min
M
Yang
Min-sheng
MS
Liu
Kai
K
Lou
D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014865:Waste Disposal, Fluid / Q000379:methods*; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins