Chromosomal aberrations in lymphocytes of lung cancer patients treated with carbon ions.
The purpose of this study is to investigate the normal tissue damage caused by carbon-ion therapy. We measured chromosomal aberrations in peripheral blood lymphocytes before, during, and after radiotherapy, using the techniques of fluorescence in situ hybridization (FISH) and chemically induced premature chromosome condensation (PCC). Twenty-two lung cancer patients treated at HIMAC (Heavy Ion Medical Accelerator in Chiba) entered the study and signed an informed consent. Frequencies of lymphocytes with chromosomal aberrations at the end of carbon-ion therapy varied among the patients. This frequency was significantly correlated to the radiation field size and weakly correlated to the counts of white blood cells and lymphocytes during the treatment. As a result, we have found that chromosomal aberrations in peripheral blood lymphocytes from patients treated for lung cancer by carbon-ions were dependent on target volume, possibly reflecting the increased involvement of lymph nodes in the target field.
Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, Chiba, Japan.
Ryonfa
R
Lee
Shigeru
S
Yamada
Naoyoshi
N
Yamamoto
Tadaaki
T
Miyamoto
Koichi
K
Ando
Marco
M
Durante
Hirohiko
H
Tsujii
D000368:Aged; D000369:Aged, 80 and over; D002247:Carbon Isotopes / Q000009:adverse effects / Q000627:therapeutic use; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002877:Chromosomes, Human / Q000528:radiation effects* / Q000648:ultrastructure; D005260:Female; D063193:Heavy Ion Radiotherapy; D020450:Heavy Ions / Q000009:adverse effects*; D006801:Humans; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000097:blood / Q000150:complications / Q000473:pathology / Q000532:radiotherapy*; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D011832:Radiation Injuries / Q000097:blood* / Q000209:etiology / Q000473:pathology*; D011878:Radiotherapy / Q000009:adverse effects
D002247:Carbon Isotopes