Daily injection of insulin attenuated impairment of liver circadian clock oscillation in the streptozotocin-treated diabetic mouse.
Recent studies have shown a dampened amplitude of clock gene rhythm in the heart and liver of streptozotocin (STZ)-treated rats and mice, however it is unknown whether impairment is due to dysfunction of the suprachiasmatic nucleus (SCN) or not. Rhythmic expression of mPER2 was dampened in the STZ-treated mouse liver but not SCN and cerebral cortex. Injection of insulin could normalize an impairment of mPer2 and mPER2 expression rhythm in the liver, when it was injected at nighttime, but not at daytime. In the present study, we demonstrated that insulin-dependent diabetes impaired oscillation of the peripheral clock gene and its product. Insulin injection can recover dampened oscillation of the peripheral clock depending on its injection time.
Department of Pharmacology, School of Science and Engineering, Waseda University, Higashifusimi 2-7-5, Nishitokyo-Shi 202-0021, Japan.
Koji
K
Kuriyama
Kei
K
Sasahara
Takashi
T
Kudo
Shigenobu
S
Shibata
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009687:Nuclear Proteins / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D056950:Period Circadian Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors
D001786:Blood Glucose; D018797:Cell Cycle Proteins; D007328:Insulin; D009687:Nuclear Proteins; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D014157:Transcription Factors