Glutamine supplemented parenteral nutrition prevents intestinal ischemia- reperfusion injury in rats.
OBJECTIVE
To examine whether glutamine prevents the injury to the intestinal mucosa after intestinal ischemia-reperfusion (I/R) in rats.


METHODS
Thirty male Sprague-Dawley rats were randomly divided into 3 groups: a standard parenteral nutrition (PN) group (n = 10); an I/R-PN group (n = 10); an I/R-glutamine enriched PN (I/R-Gln) group (n = 10). The superior mesenteric artery (SMA) was clamped. After 60 min of ischemia, reperfusion was initiated and infusion was started. All rats received isocaloric and isonitrogenous nutritional support for 48 h. Spleen, liver, mesenteric lymph nodes (MLN), and intestinal segments were removed for morphological and biochemical analyses, and blood samples were collected for bacterial culture and measurement of endotoxin levels. The permeability of intestinal mucosa was assayed by measurement of D-(-)-lactate levels in plasma.


RESULTS
In I/R-PN group, extensive epithelial atrophy was observed, mucosal thickness, villous height, crypt depth and villous surface area were decreased significantly compared with PN group, whereas these findings did not occur in the I/R-Gln group. The incidence of intestinal bacterial translocation to spleen, liver, MLN, and blood was significantly higher in I/R-PN group than that in other groups. Plasma endotoxin levels significantly increased in the I/R-PN group compared with the I/R-Gln group. Remarkably higher values of D-(-)-lactate were also detected in PN group compared with that in I/R-Gln group.


CONCLUSIONS
Glutamine protects the morphology and function of intestinal mucosa from injury after I/R in rats.
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China. wugh@zshospital.net
Guo-Hao
GH
Wu
Hao
H
Wang
Yan-Wei
YW
Zhang
Zhao-Han
ZH
Wu
Zhao-Guang
ZG
Wu
D000818:Animals; D001284:Atrophy; D018988:Bacterial Translocation; D005973:Glutamine / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000473:pathology; D007422:Intestines / Q000473:pathology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D010288:Parenteral Nutrition; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000178:diet therapy / Q000188:drug therapy* / Q000401:mortality / Q000517:prevention & control*; D015996:Survival Rate
D005973:Glutamine; D019344:Lactic Acid