The Salmonella enterica serovar typhimurium divalent cation transport systems MntH and SitABCD are essential for virulence in an Nramp1G169 murine typhoid model.
Nramp1 is a transporter that pumps divalent cations from the vacuoles of phagocytic cells and is associated with the innate resistance of mice to diverse intracellular pathogens. We demonstrate that sitA and mntH, genes encoding high-affinity metal ion uptake systems in Salmonella enterica serovar Typhimurium, are upregulated when Salmonella is internalized by Nramp1-expressing macrophages and play an essential role in systemic infection of congenic Nramp1-expressing mice.
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3 Canada.
Michelle L
ML
Zaharik
Vivian Li
VL
Cullen
Angela M
AM
Fung
Stephen J
SJ
Libby
Sonya L
SL
Kujat Choy
Bryan
B
Coburn
David G
DG
Kehres
Michael E
ME
Maguire
Ferric C
FC
Fang
B Brett
BB
Finlay
D000818:Animals; D001426:Bacterial Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004195:Disease Models, Animal; D006801:Humans; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D012486:Salmonella typhimurium / Q000378:metabolism / Q000472:pathogenicity*; D014435:Typhoid Fever / Q000382:microbiology; D014774:Virulence; D000099173:Natural Resistance-associated Macrophage Protein 1
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1; C433793:MntH protein, bacteria; C473779:SitA protein, Shigella flexneri