[Cryptosporidium oocyst viability and infectivity evaluation by flow cytometry].
Evaluating waterborne Cryptosporidium sp. oocyst infectivity is presently a major Issue for the estimation of environmental risks. The aim of this presentation, was to describe a new model suitable for determining in vivo oocyst infectivity. In this model, infection was assessed in suckling mice seven days after oocyst ingestion by measuring the number of gut oocysts using flow cytometry. Four days old mice were orally infected by serially diluted C. parvum oocyst suspensions. This model was found highly sensitive since ingestion of 1-10 oocysts resulted in infection in 70% of animals. Assays with Cryptosporidium oocysts from the environment suggest that this model may contribute to the evaluation of environmental risks due to the parasite.
Laboratoire de Parasitologie ADEN, EA 3234, Faculté de médecine et de pharmacie de Rouen, 76031 Rouen Cedex.
A
A
Delaunay
A
A
Baishanbo
L
L
Favennec
G
G
Gargala
D000818:Animals; D000833:Animals, Suckling; D016785:Cryptosporidium parvum / Q000472:pathogenicity* / Q000502:physiology*; D005434:Flow Cytometry; D008279:Magnetic Resonance Imaging; D051379:Mice; D033661:Oocysts / Q000472:pathogenicity* / Q000502:physiology*