Targeting liposomes to tumor endothelial cells for neutron capture therapy.
The aim of our work is to target (10)B to the tumor vasculature for neutron capture therapy (NCT). Alpha (v)-integrin specific RGD-peptides were coupled to liposomes that encapsulated dodecahydrododecaborate. These RGD-liposomes strongly associated with human umbilical vein endothelial cells (HUVEC) expressing this integrin and were internalized. Proliferating HUVEC proved sensitive to treatment with gamma-irradiation resulting in decreased cell viability and pronounced inhibition of DNA-synthesis with increasing dose. Irradiation of RGD-(10)B-liposome incubated HUVEC with neutrons strongly inhibited endothelial cell viability. These results suggest that efficient NCT can be achieved by targeting (10)B-liposomes to angiogenic endothelium in tumors.
Koning
Gerben A
GA
Department of Radiochemistry, Interfaculty Reactor Institute, Delft University of Technology, Mekelweg 15, NL-2629JB Delft, The Netherlands. g.a.koning@iri.tudelft.nl
Fretz
Marjan M
MM
Woroniecka
Urszula
U
Storm
Gert
G
Krijger
Gerard C
GC
D001895:Boron / Q000008:administration & dosage; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000528:radiation effects*; D005720:Gamma Rays / Q000627:therapeutic use; D006801:Humans; D007554:Isotopes / Q000008:administration & dosage; D008081:Liposomes; D009369:Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D009389:Neovascularization, Pathologic / Q000532:radiotherapy; D009842:Oligopeptides / Q000008:administration & dosage
D004273:DNA, Neoplasm; D007554:Isotopes; D008081:Liposomes; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D001895:Boron