Ceacam1a-/- mice are completely resistant to infection by murine coronavirus mouse hepatitis virus A59.
CEACAM1a glycoproteins are members of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family. Isoforms expressing either two or four alternatively spliced Ig-like domains in mice have been found in a number of epithelial, endothelial, or hematopoietic tissues. CEACAM1a functions as an intercellular adhesion molecule, an angiogenic factor, and a tumor cell growth inhibitor. Moreover, the mouse and human CEACAM1a proteins are targets of viral or bacterial pathogens, respectively, including the murine coronavirus mouse hepatitis virus (MHV), Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis, as well as Moraxella catarrhalis in humans. We have shown that targeted disruption of the Ceacam1a (MHVR) gene resulting in a partial ablation of the protein in mice (p/p mice) led to reduced susceptibility to MHV-A59 infection of the modified mice in the BALB/c background. We have now engineered and produced a Ceacam1a-/- mouse that exhibits complete ablation of the CEACAM1a protein in every tissue where it is normally expressed. We report that 3-week-old Ceacam1a-/- mice in the C57BL/6 genetic background are fully resistant to MHV-A59 infection by both intranasal and intracerebral routes. Whereas virus-inoculated wild-type +/+ C57BL/6 mice showed profound liver damage and spinal cord demyelination under these conditions, Ceacam1a-/- mice displayed normal livers and spinal cords. Virus was recovered from liver and spinal cord tissues of +/+ mice but not of -/- mice. These results indicate that CEACAM1a is the sole receptor for MHV-A59 in both liver and brain and that its deletion from the mouse renders the mouse completely resistant to infection by this virus.
Department of Microbiology, University of Colorado Health Sciences Center, Denver, USA.
Erin
E
Hemmila
Claire
C
Turbide
Melanie
M
Olson
Serge
S
Jothy
Kathryn V
KV
Holmes
Nicole
N
Beauchemin
D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000502:physiology; D015815:Cell Adhesion Molecules; D018352:Coronavirus Infections / Q000209:etiology / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004247:DNA / Q000235:genetics; D018390:Gene Targeting; D006023:Glycoproteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D006524:Hepatitis, Viral, Animal / Q000209:etiology / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D006517:Murine hepatitis virus / Q000472:pathogenicity*; D011991:Receptors, Virus / Q000172:deficiency* / Q000235:genetics / Q000502:physiology
D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D011991:Receptors, Virus; D004247:DNA