Protollin: a novel adjuvant for intranasal vaccines.
Protollin is a novel adjuvant comprising Proteosomes non-covalently complexed with LPS. Intranasal immunization of mice with Protollin combined with detergent-split influenza antigens (HA) or recombinant influenza hemagglutinin (rHA) enhanced serum IgG and mucosal IgA levels by up to 250-fold compared with immunization with the antigens alone. IFN-gamma responses were also enhanced compared to the levels produced by splenocytes from mice immunized with antigen alone, while production of IL-5 was abrogated. Mice immunized with Protollin-rHA were completely protected against lethal challenge with influenza virus, demonstrating that Protollin is an effective mucosal adjuvant for prophylactic vaccines.
Jones
Taff
T
ID Biomedical Corporation of Quebec, 7150 Frederick Banting, Ville St-Laurent, Montreal, QC, Canada H4S 2A1. tjones@idbiomedical.com
Cyr
Sonya
S
Allard
Francine
F
Bellerose
Nathalie
N
Lowell
George H
GH
Burt
David S
DS
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D002626:Chemistry, Pharmaceutical; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D004337:Drug Carriers; D004338:Drug Combinations; D005260:Female; D006388:Hemagglutinins / Q000032:analysis / Q000276:immunology; D018928:Immunity, Mucosal / Q000187:drug effects*; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007252:Influenza Vaccines / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control; D013154:Spleen / Q000166:cytology; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D004337:Drug Carriers; D004338:Drug Combinations; D006388:Hemagglutinins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D008070:Lipopolysaccharides; D008081:Liposomes; C493785:Protollin; D014612:Vaccines; D014614:Vaccines, Synthetic; D003546:Cysteine Endopeptidases