[Association of APOA5 gene single nucleotide polymorphism with levels of lipids and coronary heart disease in Chinese].
OBJECTIVE
To investigate the single nucleotide polymorphism 4 (SNP4) of the apolipoprotein A5 (APOA5) gene possible association with coronary heart disease(CHD) and its distribution of in Chinese Han population.


METHODS
APOA5 SNP4 genotyping was performed using polymerase chain reaction and Hae III restriction fragment length polymorphism analysis.


RESULTS
APOA5 allelic frequencies of T, C were 0.435, 0.565 and 0.374, 0.626 in CHD group and control group, respectively. There is significant difference in allele and genotype frequencies between CHD group and control group (P<0.05). The levels of plasma high density lipoprotein in CHD patients with CC genotype were higher than those in CHD patients with other genotypes (P<0.01). The frequencies of T allele and C allele in Chinese was significantly different from those in Caucasians (0.374 vs 0.663, 0.626 vs 0.337, P<0.01). The C allele was much more common in Chinese population.


CONCLUSIONS
The association is found between the Hae III polymorphism and CHD, There is a significant correlation between the CC genotype of the APOA5 and the levels of plasma high density lipoprotein-cholosteal in the CHD group.
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041 PR China.
He-kun
HK
Liu
Chun-ting
CT
Wang
Si-zhong
SZ
Zhang
Cui-ying
CY
Xiao
Xue-fei
XF
Li
Ke-lan
KL
Zhang
Li
L
Zhang
Zhi-guang
ZG
Su
Yong-xin
YX
Ma
Bin
B
Zhou
Ke-qin
KQ
Zheng
Gui-xing
GX
Li
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D008055:Lipids