[Effects of asiaticoside on cell proliferation and Smad signal pathway of hypertrophic scar fibroblasts].
OBJECTIVE
To investigate the effects of asiaticoside on the proliferation and the Smad signal pathway of the hypertrophic scar fibroblasts.


METHODS
The hypertrophic scar fibroblasts were cultured with tissue culture method. The expressions of Smad2 and Smad7 mRNA after asiaticoside treatment were determined by reverse transcription-polymerase chain reaction 48 hours later. The cell cycle, the cell proliferation, the cell apoptosis and the expression of phosphorylated Smad2 and Smad7 with (experimental group) or without (control group) asiaticoside were detected with flow cytometry, immunocytochemistry and Western blot.


RESULTS
Asiaticoside inhibited the hypertrophic scar fibroblasts from phase S to phase M. The Smad7 content and the expression of Smad7 mRNA were (1.33+/-1.26)% and (50.80+/-22.40)% in experimental group, and (9.15+/-3.36)% and (32.18+/-17.84)% in control group; there were significant differences between two groups (P<0.05). While the content and the mRNA expression of Smad2 had no significant difference between two groups.


CONCLUSIONS
Asiaticoside inhibits the scar formation through Smad signal pathway.
Department of Burns, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou Guangdong, PR China. lionps@263.net
Shu
S
Pan
Tianzeng
T
Li
Yeyang
Y
Li
D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D015398:Signal Transduction / Q000187:drug effects*; D051899:Smad2 Protein; D051906:Smad7 Protein; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D014315:Triterpenes / Q000494:pharmacology*
D004268:DNA-Binding Proteins; C497782:SMAD2 protein, human; C497806:SMAD7 protein, human; D051899:Smad2 Protein; D051906:Smad7 Protein; D015534:Trans-Activators; D014315:Triterpenes; C004446:asiaticoside