Analysis of the roles of Pins and heterotrimeric G proteins in asymmetric division of Drosophila neuroblasts.
Drosophila neuroblasts divide asymmetrically to give rise to two daughter cells of distinct fates and sizes. A number of studies in asymmetric cell division have begun to elucidate the molecular mechanisms underlying asymmetric protein/RNA localization, spindle orientation, and spindle asymmetry. This article describes methods of analyzing the role of Pins and heterotrimeric G proteins in Drosophila neuroblast asymmetric division.
Life Sciences Laboratory, National University of Singapore, Singapore.
Fengwei
F
Yu
D020816:Amino Acid Motifs; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D004330:Drosophila / Q000196:embryology / Q000502:physiology*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D017434:Protein Structure, Tertiary; D020798:Two-Hybrid System Techniques
D029721:Drosophila Proteins; D020962:Heterotrimeric GTP-Binding Proteins