Activation of eosinophils by rice-husk dust exposure: a possible mechanism for the aggravation of asthma during rice harvest.
Grain dust and other irritants affect the airway of allergic patients in rice-growing area during the harvest. The aim of this study was to elucidate the mechanism of airway hypersensitivity in rice-growing areas during the harvest. Firstly, the effect of rice-husk dust on eosinophil activation was studied. Secondary, the concentration of lipopolysaccharides (LPS), a potent activator of inflammatory cells, in rice-husk dust was measured. Since it is possible for LPS, a component of gram-negative bacterial cell wall, to adhere to the particle of smoke generated from rice-husk dust, LPS contained in the smoke was also measured. Furthermore, chemical irritants contained in the smoke generated from the rice-husk dust were analyzed. Microscopically, the dust contained fine thorns dropped off from the outer sheath of the rice, and irritated the skin, throat and eyes. The grain dust extract increased the expressions of eosinophil activation markers. These up-regulatory effects were largely dependent on LPS. The smoke contained LPS and several chemical irritants such as formaldehyde and acetaldehyde. Rice-husk dust and its smoke, hazardous air pollutants, probably play a major role in the aggravation of airway diseases in agricultural areas.
Kayaba
Hiroyuki
H
Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Hondo, Japan. kayaba@hos.akita-u.ac.jp
Meguro
Hitomi
H
Muto
Hajime
H
Kamada
Yumiko
Y
Adachi
Tetsuya
T
Yamada
Yoshiyuki
Y
Kanda
Akira
A
Yamaguchi
Kazutoshi
K
Hamada
Kazuyuki
K
Ueki
Shigeharu
S
Chihara
Junichi
J
D000293:Adolescent; D000328:Adult; D000382:Agricultural Workers' Diseases / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D004391:Dust; D004804:Eosinophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D012275:Oryza / Q000033:anatomy & histology / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D012906:Smoke
D004391:Dust; D008070:Lipopolysaccharides; D012906:Smoke