Allelic drop-out in the STR system ACTBP2 (SE33) as a result of mutations in the primer binding region.
In the course of routine genotyping of forensic reference samples by multiplex PCR, an allelic drop-out due to mutations in the primer binding regions of the highly polymorphic STR marker ACTBP2 was observed in 17 samples. The variation rate was estimated to be 0.0014 (95% confidence interval: 0.0006-0.003). The most frequently found mutation was an G to A transition in the reverse primer binding region which was present in 14 out of 17 cases. To overcome the problem we have added a modified reverse primer to different multiplex kits that led to the correct genotype.
Heinrich
Marielle
M
Institut für Rechtsmedizin, Universitätsklinikum Münster, Röntgenstrasse 23, 48149 Münster, Germany.
Müller
Miriam
M
Rand
Steven
S
Brinkmann
Bernd
B
Hohoff
Carsten
C
D001665:Binding Sites; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D017931:DNA Primers