Cloning and expression analysis of a MAPKKK gene and a novel nodulin gene of Lotus japonicus.
We isolated a cDNA encoding mitogen-activated protein kinase kinase kinase alpha, designated LjM3Kalpha, from Lotus japonicus, a model legume. The gene was expressed constitutively in roots, root nodules, and shoots. We also identified a novel nodulin gene, LjNUF, that shows specific expression in nodules. LjNUF resembles the C-terminal half of a hypothetical protein (pir//D85436), the N-terminal half of which is similar to a portion of mitogen-activated protein kinase kinase kinase gamma. Although LjNUF was predicted to be a secreted protein, its function remains to be clarified.
Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Natsuko
N
Kinoshita
Yasuhiro
Y
Ooki
Yuichi
Y
Deguchi
Svetlana A
SA
Chechetka
Hiroshi
H
Kouchi
Yosuke
Y
Umehara
Katsura
K
Izui
Shingo
S
Hata
D017343:Genes, Plant / Q000235:genetics*; D000070116:Lotus / Q000235:genetics*; D020930:MAP Kinase Kinase Kinases / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D018514:Plant Structures / Q000235:genetics*
D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D020930:MAP Kinase Kinase Kinases