Towards two-dimensional electrophoresis mapping of the cerebrospinal fluid proteome from a single individual.
We test the ability of state-of-the-art two-dimensional electrophoresis (2-DE) technology to enable the proteome mapping of ante mortem cerebrospinal fluid (CSF) from a single individual. Using the sensitive technologies of a fluorescent protein stain and fluorescence laser scanning of 2-DE gels, combined with matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry (MALDI-TOF/TOF-MS) for protein identification, a highly detailed 2-DE map of the CSF proteome was created. The 2-DE map contains 600 identified spots representing 82 different proteins. Of the 82 proteins identified, 25 have not appeared in any previously published 2-DE map of CSF, and 11 have not been previously reported to exist in CSF. Most of the identifications originate from an ante mortem CSF sample collected from a single hydrocephalus patient. A supplemental map created from neurologically normal patients is also presented. A webpage with protein identification and scoring information from both maps is available at http://www.leelab.org/csfmap.
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853-5201, USA.
Erin J
EJ
Finehout
Zsofia
Z
Franck
Kelvin H
KH
Lee
D002555:Cerebrospinal Fluid / Q000378:metabolism*; D019295:Computational Biology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006849:Hydrocephalus / Q000378:metabolism*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005456:Fluorescent Dyes; D020543:Proteome