Induction of neutralising antibodies restricts the use of human granulocyte/macrophage colony stimulating factor for vaccine studies in rhesus macaques.
Granulocyte/macrophage-colony stimulating factor (GM-CSF) is a valuable adjuvant to enhance induction of cellular immune responses in rodents. Less information is available regarding its use as an adjuvant in primates or humans. We explored recombinant human GM-CSF for potential vaccine studies in rhesus macaques and focused on its effect on peripheral monocytes as progenitors of dendritic cells and its potential immunogenicity. Application of human GM-CSF to nine animals led to an average 32-fold increase in monocyte numbers. This was not observed upon re-treatment, which coincided with GM-CSF-specific neutralising antibodies. These also neutralised the activity of rhesus macaque GM-CSF. The data underscore the need to use species-specific GM-CSF for immunomodulation in primates.
Department of Medical Microbiology and Immunology of Infection, Charité-University Medicine Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany.
Martin
M
Eisenblätter
Christiane
C
Stahl-Hennig
Seraphin
S
Kuate
Nicole
N
Stolte
Edith
E
Jasny
Helmut
H
Hahn
Melissa
M
Pope
Klara
K
Tenner-Racz
Paul
P
Racz
Ralph M
RM
Steinman
Klaus
K
Uberla
Ralf
R
Ignatius
D000818:Animals; D019138:Antibodies, Blocking / Q000096:biosynthesis* / Q000276:immunology*; D015153:Blotting, Western; D002469:Cell Separation; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors* / Q000276:immunology*; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D009000:Monocytes / Q000276:immunology; D009500:Neutralization Tests; D014612:Vaccines / Q000276:immunology*
D019138:Antibodies, Blocking; D016207:Cytokines; D014612:Vaccines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor