Micropatterns of spores displaying heterologous proteins.
Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea.
Tae Jung
TJ
Park
Kyung-Bok
KB
Lee
Seok Jae
SJ
Lee
Jong Pil
JP
Park
Zee-Won
ZW
Lee
Soo-Keun
SK
Choi
Heung-Chae
HC
Jung
Jae-Gu
JG
Pan
Sang Yup
SY
Lee
Insung S
IS
Choi
D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013171:Spores, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins