A new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes.
By performing cDNA AFLP on pre- and early parasitic juveniles, we identified genes encoding a novel type of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in the cyst nematode Heterodera schachtii. The proteins consist of three domains, a signal peptide for secretion, a mono-ubiquitin domain, and a short C-terminal positively charged domain. A gfp-fusion of this protein is targeted to the nucleolus in tobacco BY-2 cells. We hypothesize that the C-terminal peptide might have a regulatory function during syncytium formation in plant roots.
Tytgat
Tom
T
Department of Biology, Section Nematology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Vanholme
Bartel
B
De Meutter
Jan
J
Claeys
Myriam
M
Couvreur
Marjolein
M
Vanhoutte
Isabelle
I
Gheysen
Greetje
G
Van Criekinge
Wim
W
Borgonie
Gaetan
G
Coomans
August
A
Gheysen
Godelieve
G
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D009348:Nematoda / Q000235:genetics* / Q000472:pathogenicity; D010614:Pharynx / Q000166:cytology / Q000378:metabolism; D018517:Plant Roots / Q000469:parasitology; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D014026:Nicotiana / Q000235:genetics / Q000469:parasitology*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D015801:Helminth Proteins; D025801:Ubiquitin