Direct evidence of interaction of a green tea polyphenol, epigallocatechin gallate, with lipid bilayers by solid-state Nuclear Magnetic Resonance.
The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solid-state (31)P and (2)H NMR. The (31)P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The (2)H NMR spectrum of [4-(2)H]EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These (31)P and (2)H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.
Laboratory of Functional Food Science and COE Program in the 21st Century, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan.
Shigenori
S
Kumazawa
Katsuko
K
Kajiya
Akira
A
Naito
Hazime
H
Saito
Satoru
S
Tuzi
Michikazu
M
Tanio
Masayuki
M
Suzuki
Fumio
F
Nanjo
Eri
E
Suzuki
Tsutomu
T
Nakayama
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*
D005419:Flavonoids; D008051:Lipid Bilayers; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D002392:Catechin; D003903:Deuterium; C045651:epigallocatechin gallate; D004134:Dimyristoylphosphatidylcholine