Enzyme inhibition by the molluscicidal agent Punica granatum Linn. bark and Canna indica Linn. root.
Sublethal in vivo 24 h exposure to (40% and 80% of 24 h LC(50)) active fractions of Punica granatum bark or Canna indica root or in combination with other plant-derived molluscicides significantly inhibited the activity of acetylcholinesterase, acid/alkaline phosphatase, Na(+)K(+)ATPase and lactic dehydrogenase in the nervous tissue of Lymnaea acuminata. The inhibition kinetics of these enzymes indicates that active fractions of both the plants caused a competitive inhibition of AChE, LDH, ALP, ACP and Na(+)K(+)ATPase.
Tripathi
Sanjay M
SM
Department of Zoology, DDU Gorakhpur University, India.
Singh
V K
VK
Singh
Sanjay
S
Singh
D K
DK
D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008195:Lymnaea / Q000187:drug effects* / Q000201:enzymology; D029561:Lythraceae; D008975:Molluscacides / Q000494:pharmacology*; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D019862:Zingiberales
D004791:Enzyme Inhibitors; D008975:Molluscacides; D010936:Plant Extracts; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases