Microfluidic ELISA: on-chip fluorescence imaging.
Fluorescent reactions of a heterogeneous sandwich enzyme-linked immunoassay (ELISA) in an all-PDMS [poly (dimethylsiloxane)] microfluidic device were detected using a cooled charge coupled device (CCD) camera interfaced with an epifluorescence microscope. The study represents preliminary efforts to integrate biochemical reactions and detection on-chip using the "hybrid" detection approach. In initial experiments, the PDMS chip microsensor was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17nM. Thus, we demonstrate here the extension of this hybrid integrated technique to on-chip imaging and quantification of light emission from a biochemical immunoassay in PDMS chip.
Department of Chemical Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. eddyetes@adelphia.net
Edward
E
Eteshola
Michal
M
Balberg
D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007075:Immunoglobulin M / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D012828:Silicones / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; D007075:Immunoglobulin M; D012828:Silicones; C013830:baysilon