Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction.
Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is an efficient tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of genomic sequences flanking DNA (T-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.
South China Agricultural University, College of Life Science, Guangzhou, China.
Yao-Guang
YG
Liu
Yuanlin
Y
Chen
Qunyu
Q
Zhang
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013816:Thermodynamics
D017931:DNA Primers; D004269:DNA, Bacterial; C036483:T-DNA