Effect of poly(ethylene imine) molecular weight and pegylation on organ distribution and pharmacokinetics of polyplexes with oligodeoxynucleotides in mice.
The in vivo body distribution and the pharmacokinetics of a 20mer double-stranded nuclear factor kappaB decoy oligodeoxynucleotide (ODN) complexed with 25-kDa poly(ethylene imine) (PEI), low molecular weight 2.7-kDa PEI, and PEGylated PEI [bPEI(25k)-glPEG(550)(50)] after intravenous injection were studied in BALB/c mice using a double-labeling technique to follow simultaneously the distribution of both complex components. The polymers were radioactively labeled with (125)I by Bolton-Hunter reagent and the decoys with [gamma-(32)P]ATP by an enzymatic 5'-end-labeling technique. After i.v. bolus injections into the jugular vein, organ samples were taken after 15 min, 2 h and 12 h. For pharmacokinetic studies blood and plasma samples were collected from 20 s up to 2 h. Uncomplexed decoy was found to be degraded already after 15 min and was rapidly eliminated renally into urine. Complexation with the homopolymers increased the organ levels and circulation time of ODN after 15 min, with similar organ distribution profiles for (125)I and (32)P. In contrast to the behavior of free ODN, the complexes were mainly distributed into liver and spleen. Whereas the organ concentrations of (125)I remained high over 12 h, the (32)P values of ODN decreased in a time-dependent manner, likely due to separation of the complexes and degradation of the DNA. Although PEGylated PEI demonstrated a slower (125)I-uptake into the RES organs compared with 25-kDa PEI due to the shielding effect of PEG [poly(ethylene glycol)], it was not able to better stabilize the complexes in the circulation or protect DNA from degradation.
Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, USA. fischerd@staff.uni-marburg.de
Dagmar
D
Fischer
Berit
B
Osburg
Holger
H
Petersen
Thomas
T
Kissel
Ulrich
U
Bickel
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019540:Area Under Curve; D004249:DNA Damage; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012157:Mononuclear Phagocyte System / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000493:pharmacokinetics*; D016376:Oligonucleotides, Antisense / Q000097:blood / Q000493:pharmacokinetics*; D010761:Phosphorus Radioisotopes; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics*; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010761:Phosphorus Radioisotopes; D011108:Polymers; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D011094:Polyethyleneimine