SARS coronavirus detection.
We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.
Robert Koch-Institut, Berlin, Germany. nitschea@rki.de
Andreas
A
Nitsche
Brunhilde
B
Schweiger
Heinz
H
Ellerbrok
Matthias
M
Niedrig
Georg
G
Pauli
D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000821:virology*
D012367:RNA, Viral