Cell attachment of periodontal ligament cells on commercially pure titanium at the early stage.
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
Center of Stomatology, Tongji Hospital, Tongji medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Bin
B
Zhou
Yingguang
Y
Cao
Lijuan
L
Wu
Yanxiang
Y
Yuan
Yinping
Y
Zeng
D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion; D021982:Cell-Matrix Junctions; D002478:Cells, Cultured; D015921:Dental Implants; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium