Homing-associated cell adhesion molecules and cell cycle status on the nucleated cells in the bone marrow, mobilized peripheral blood and cord blood.
Homing-associated cell adhesion molecules (H-CAM) on the CD34+ cells play an important role for the engraftment process following hematopoietic stem cell transplantation (HSCT). However, it seems that not only CD34+ cells but also other nucleated cells (NCs) with H-CAM could be implicated in the engraftment process and the proliferation of hematopoietic stem cells. We investigated the differences of HCAM and cell cycle status on the NCs in cord blood (CB), bone marrow (BM), and mobilized peripheral blood (PB). The proportions of CXCR4+ cells within the NC populations were greater in CB than in PB or BM (p=0.0493), although the proportions of CXCR4+, CD44+, and CD49d+ cells within the CB CD34+ cell populations were same within BM or PB. A lower proportion of CD34+CD49d+ cells within the CD34+ cell populations was more noted in CB than in PB or BM (p=0.0085). There were no differences in cell cycle status between CB and BM or PB. Our results suggest that the migrating potential of CB would be enhanced with increased CXCR4 expression on the NCs, but the adhesion potential of CB CD34+ cells would be less than that of PB and BM. These findings may help explain why the lower cell dose is required and engraftment is delayed in cord blood stem cell transplantation.
Lee
Young-Ho
YH
Hematopoietic Stem Cell Transplantation Program, Dong-A Cancer Center, Dong-A University Medical Center, Busan, Korea. yhlee1@daunet.donga.ac.kr
Lee
Young-Ah
YA
Noh
Kyu-Tae
KT
Kim
Kyeong-Hee
KH
Han
Jin-Yeong
JY
Seo
Su-Yeong
SY
Kwon
Hyuk-Chan
HC
Kim
Jae-Seok
JS
Kim
Hyo-Jin
HJ
D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D039441:Integrin alpha4 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism
D018952:Antigens, CD34; C497483:CD44 protein, human; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D019718:Receptors, CXCR4; D039441:Integrin alpha4