Evaluation of cardioprotective effects of recombinant soluble P-selectin glycoprotein ligand-immunoglobulin in myocardial ischemia-reperfusion injury by real-time myocardial contrast echocardiography.
OBJECTIVE
The purpose was to examine the cardioprotective effects of recombinant P-selectin glycoprotein ligand-immunoglobulin (rPSGL-Ig) in ischemia-reperfusion injury by real-time myocardial contrast echocardiography (MCE).


BACKGROUND
P-selectin mediates leukocyte recruitment into areas of inflammation.


METHODS
Sixteen pigs underwent 45 min of left anterior descending coronary artery occlusion followed by reperfusion and received rPSGL-Ig or vehicle. To assess changes in myocardial perfusion (A x beta), MCE was performed.


RESULTS
After 120 min of reperfusion, A x beta in the risk area was higher (0.84 +/- 0.15 dB/s vs. 0.28 +/- 0.1 dB/s, p < 0.0001), and the infarct size was lower (30.3 +/- 12% vs. 57 +/- 14%, p = 0.002) in the rPSGL-Ig group compared with the vehicle group.


CONCLUSIONS
Recombinant PSGL-Ig improved postischemic reflow accurately detected by real-time MCE.
Department of Cardiology, University of Heidelberg, Heidelberg, Germany. alexander_hansen@med.uni-heidelberg.de
Alexander
A
Hansen
Anjali
A
Kumar
David
D
Wolf
Kamilla
K
Frankenbergerova
Arthur
A
Filusch
Marie-Luise
ML
Gross
Sebastian
S
Mueller
Hugo
H
Katus
Helmut
H
Kuecherer
D000818:Animals; D003287:Contrast Media; D004195:Disease Models, Animal; D004452:Echocardiography; D008562:Membrane Glycoproteins / Q000627:therapeutic use*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000517:prevention & control*; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000627:therapeutic use; D015427:Reperfusion Injury / Q000000981:diagnostic imaging / Q000517:prevention & control*; D013552:Swine
D003287:Contrast Media; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011994:Recombinant Proteins