Contrasting effects of coplanar versus non-coplanar PCB congeners on immunomodulation and CYP1A levels (determined using an adapted ELISA method) in the common sea star Asterias rubens L.
Biological effects of two structurally contrasting PCB congeners (coplanar 77 and non coplanar 153) were investigated by measuring the induction of CYP1A immunopositive protein (CYP1A IPP) in the pyloric caeca and the production of reactive oxygen species (ROS) by amoebocytes in the common sea star Asterias rubens. CYP1A IPP was quantified using a specially designed ELISA which uses competitive binding between sea stars and trout CYP1A IPPs. Only the coplanar congener had a significant effect on the two considered biological responses. Intensity of the effects was dose-dependent. However, the highest dose of PCB 77 induced a dramatic decrease of ROS production. It is concluded that coplanar PCBs straightforwardly affect key biological processes such as the immune system and mixed-function oxidase (MFO) system.
Danis
Bruno
B
Laboratoire de Biologie marine (CP 160/15), Université Libre de Bruxelles, Av. F.D. Roosevelt 50, B-1050 Brussels, Belgium. bdanis@ulb.ac.be
Goriely
Stanislas
S
Dubois
Philippe
P
Fowler
Scott W
SW
Flamand
Véronique
V
Warnau
Michel
M
D000818:Animals; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011708:Pylorus / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013215:Starfish / Q000276:immunology* / Q000378:metabolism*; D023382:Toxicity Tests, Acute
D017382:Reactive Oxygen Species; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1