Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings.
Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century.
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic. radovan.hynek@vscht.cz
Radovan
R
Hynek
Stepanka
S
Kuckova
Janka
J
Hradilova
Milan
M
Kodicek
D000818:Animals; D002417:Cattle; D010150:Paint / Q000032:analysis*; D010151:Paintings; D010860:Pigments, Biological / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010860:Pigments, Biological; D011506:Proteins