A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes.
This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.
Johnson-Brousseau
Sheila A
SA
Plant Gene Expression Center and Plant and Microbial Biology, USDA/ARS and UC-Berkeley, 800 Buchanan St., Albany, CA 94710, USA. sjb@uclink.berkeley.edu
McCormick
Sheila
S
D017360:Arabidopsis / Q000235:genetics*; D003433:Crosses, Genetic; D015870:Gene Expression; D010641:Phenotype; D011058:Pollen / Q000235:genetics* / Q000254:growth & development