Inhibitory effect of Bunium persicum on histamine (H1) receptors of guinea pig tracheal chains.
The antihistaminic effects of aqueous and macerated extracts, essential oil, 20 nM chlorpheniramine, and saline were tested by performing the cumulative log concentration-response curves of histamine-induced contraction of isolated guinea pig tracheal chains incubated with three different conditions including: (1) 1.4 microM indomethacin, (2) indomethacin, 1 microM propranolol, and 10 nM atropine, and (3) indomethacin and propranolol (for each group n = 8). The results showed clear parallel rightward shifts in histamine-response curves obtained in the presence of macerated extract in group 2, aqueous extract in group 3, and essential oil in groups 2 and 3 experiments compared with the curves obtained in the presence of saline. The EC50 (effective concentration of histamine causing 50% of maximum response) obtained in the presence of essential oil, extracts, and chlorpheniramine in all three sets of experiments were significantly higher than that of saline (P<0.05 to p<0.001). The maximum response obtained in the presence of aqueous extract in group 3 compared to group I and that of macerated extract in group 2 compared to the other two sets of experiments were improved. These results indicated a competitive antagonistic effect of Bunium persicum at histamine H1 receptors.
Department of Physiology, Ghlaem Medical Centre, Mashhad University of Medical Sciences, Mashhad 91735, Iran. mhboskabady@hotmail.com
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D000818:Animals; D019661:Apiaceae; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006632:Histamine; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D011969:Receptors, Histamine H1 / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects
D006634:Histamine H1 Antagonists; D010936:Plant Extracts; D011969:Receptors, Histamine H1; D006632:Histamine