[Role of interleukin-9 in the pathogenesis of chronic obstructive pulmonary disease].
OBJECTIVE
To study the role of interleukin-9 in the airway inflammation from patients with COPD.


METHODS
Induced sputum was obtained from 30 COPD patients with stable disease(group A),31 asthmatics patients with stable disease(group B) and 15 healthy individuals(group C). IL-9,IL-5 and IL-8 in sputum supernatants were measured by sandwich enzyme-linked immunosorbent assay(ELISA) and IL-9 positive expression and quantitative analysis were conducted by Streptavidin peroxidase method and image analysis technology.


RESULTS
The levels of IL-9 in group A and B were all significantly higher than those in groups C. IL-9 positive expression mainly located in the cytoplasm of macrophages. The positive rates of IL-9 in group A and B were all significantly higher than that of group C (chi(2)=20.821, 19.908, P<0.001 . The percentage of neutrophil and the level of IL-8 in group A was significantly higher than those of groups B and C. The level of IL-9 was positively correlated with the number of macrophages in group A(r=0.407,P=0.039).


CONCLUSIONS
The level of IL-9 from the patients with COPD was significantly higher than that of healthy individuals and correlated with the number of macrophages and the level of IL-8. This suggests that IL-9 may be used as a new diagnostic marker in airway inflammation of COPD.
Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100083, China.
Zheng
Z
Liu
Wan-zhen
WZ
Yao
Yan
Y
Chen
Yan-ling
YL
Ding
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D016906:Interleukin-9 / Q000096:biosynthesis* / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000503:physiopathology; D013183:Sputum / Q000378:metabolism*
D016209:Interleukin-8; D016906:Interleukin-9