Interleukin-11 as an osteoprotegerin-inducing factor in culture medium of blastic cells from a patient with acute megakaryocytic leukemia complicated with osteosclerosis.
We came across a rare case of acute megakaryocytic leukemia, the clinical course of which was relatively chronic and nonaggressive. This case was complicated with generalized severe osteosclerosis (OS). The medium in which blastic cells from the patient were cultured showed a strong activity to enhance the expression of an osteosclerotic cytokine, osteoprotegerin (OPG), as revealed by real-time quantitative RT-PCR and Western blot analysis. The OPG-inducing activity of the culture medium was neutralized by the anti-interleukin-11 (IL-11) antibody. These results indicate that IL-11 produced by the blasts was a causative factor of the OS observed in this patient.
Department of Internal Medicine (Section 4), Sapporo Medical University, School of Medicine, Sapporo, Japan.
Tsutomu
T
Sato
Takuya
T
Matsunaga
Masaya
M
Kida
Kazuhiro
K
Morii
Takuro
T
Machida
Yutaka
Y
Kawano
Kiminori
K
Nakamura
Kageaki
K
Kuribayashi
Kohichi
K
Takada
Satoshi
S
Iyama
Yasushi
Y
Sato
Tetsuji
T
Takayama
Minoru
M
Takahashi
Junji
J
Kato
Manabu
M
Chokki
Yoshiro
Y
Niitsu
D000328:Adult; D001752:Blast Crisis / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D017370:Interleukin-11 / Q000502:physiology*; D007947:Leukemia, Megakaryoblastic, Acute / Q000150:complications* / Q000473:pathology; D008297:Male; D053244:Osteoprotegerin; D010026:Osteosclerosis / Q000209:etiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D006023:Glycoproteins; D017370:Interleukin-11; D053244:Osteoprotegerin; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human