Experimental lagochilascariosis: histopathological study of inflammatory response to larval migration in the Murine model.
The goal of this study was to investigate the pattern of inflammatory response induced by Lagochilascaris minor in murine experimental model. For this purpose 115 mice were given 1000-3000 L. minor infective eggs "per os" and 51 uninfected mice were considered as controls. Four hours post-inoculation (PI), 3rd stage larvae were seen passing through the mucosa of terminal ends of small intestine. Six hours PI larvae were observed as an embolus inside the portal vein and also migrating through the liver parenchyma. During the first 24 h larvae-containing eggs of L. minor were observed in the lumen of intestinal tract. Two days PI larvae were seen migrating through lung parenchyma associated with an initial neutrophilic perivasculitis. From the 13th day of this experimental study, L. minor larvae were found mainly in skeletal muscles, in the center of granulomas. Concentric fibrosis with mixed inflammatory infiltrate involved the larvae after the 47th day PI, persistently. This experimental murine study with L. minor indicated that the 3rd stage larvae penetrated via ileum-cecal mucosa reaching the liver and probably other tissues through the hematogenic via. Throughout its pathway the larvae induced a granulomatous reaction, with abundant polimorphonuclear cells.
Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, 74605-050, Brazil.
Amélia Regina
AR
Semerene
Ruy de Souza
Rde S
Lino
Jayrson Araújo
JA
Oliveira
Albino Verçosa
AV
Magalhães
Mariane Martins Araújo
MM
Stefani
Alverne Passos
AP
Barbosa
Dulcinéa Maria Barbosa
DM
Campos
D000328:Adult; D000818:Animals; D002648:Child; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007814:Larva / Q000254:growth & development / Q000472:pathogenicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009348:Nematoda / Q000254:growth & development* / Q000472:pathogenicity; D009349:Nematode Infections / Q000469:parasitology*; D013997:Time Factors