An immunohistochemical study on the neuroendocrine system in the alimentary canal of the brown trout, Salmo trutta, L., 1758.
Several neurohormonal peptides of the gastrointestinal system of fish have been revealed by immunohistochemical methods. Among salmonids, the rainbow trout, Oncorhynchus mykiss (Walbaum) is the most studied species, whereas the informations about other species of the taxonomic group are lacking. The regional distribution and relative densities of cells belonging to the neuroendocrine system have been in this paper demonstrated in the gut of the brown trout, Salmo trutta Linnaeus. In the gastric mucosa, endocrine cells were detected, which were immunoreactive to bombesin-, gastrin-, and secretin-antisera. Endocrine cells containing gastrin-, bombesin-, cholecystokinin-8-, glucagon-, and leptin-like immunoreactivities were present in the pyloric caeca and intestine. The pancreatic endocrine islets contained glucagon-, and, possibly, secretin-like-immunoreactive endocrine cells, as well as a contingent of galanin-like-immunoreactive nerve fibres. The exocrine pancreatic parenchyma showed bombesin-like-immunoreactive nerve fibres. Within the tested regulatory peptides, bombesin and leptin were observed in both endocrine cells and nerve cell bodies and fibres. Leptin was in addition detected in epithelial cells of the gastric glands. In the brown trout we have never observed any immunoreactivity to the VIP antiserum (either in the stomach or in the intestine). Some special structural patterns (in particular those ones related to galanin- and leptin-immunohistochemical data) have thus been detected for the first time in the brown trout, and provide further data for a better knowledge of gut morpho-functional aspects in this economically important fish.
Bosi
Giampaolo
G
Department of Veterinary Sciences and Technologies for Food Safety, University of Milan, via Trentacoste n. 2, I-20134 Milan, Italy.
Di Giancamillo
Alessia
A
Arrighi
Silvana
S
Domeneghini
Cinzia
C
D000818:Animals; D001839:Bombesin / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism*; D004068:Digestive System Physiological Phenomena; D017615:Enteric Nervous System / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D007150:Immunohistochemistry; D020738:Leptin / Q000378:metabolism; D009490:Neurosecretory Systems / Q000378:metabolism*; D036361:Peptide Hormones / Q000378:metabolism*; D012633:Secretin / Q000378:metabolism; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*
D005755:Gastrins; D005768:Gastrointestinal Hormones; D020738:Leptin; D036361:Peptide Hormones; D012633:Secretin; D005934:Glucagon; D001839:Bombesin