Histological and morphometrical examinations of suprarenal glands in rats after experimental administration of atorvastatin and ethanol.
The experiment was carried out on Wistar rat males weighting about 250 g. Animals from control group I received water and standard granulated fodder ad libitum. Animals from control group II received 20% ethanol instead of water. Animals from experimental group I received Atorvastatin in the dose of 0.28 mg/24h, animals from experimental group II received Atorvastatin in the dose of 2.8 mg/24h, animals from experimental group III received 20% ethanol + Atorvastatin in the dose of 0.28 mg. After 6 weeks the animals were decapitated. H+E staining, PAS method for detection of neutral mucopolysaccharides and Thomas's reaction for detection of lipids were made on 6 micron thick sections. The surface areas of the nuclei sections were assessed in the zona fasciculata. It was stated that ethanol causes strong hyperemia of suprarenal cortex, the high dose of atorvastatin--2.8 mg/24h and small dose of atorvastatin--0.28 mg/24h administered with ethanol cause the decrease of lipid amount in the whole suprarenal cortex and degenerative changes in many cells of the zona fasciculata.
Zarebska
Alicja
A
Department and Institute of Histology and Embryology with the Laboratory for Experimental Cytology, Medical University of Lublin, Lublin, Poland.
Izdebska
Magdalena
M
Sekita-Krzak
Joanna
J
Matusiewicz
Janusz
J
Visconti
Józef
J
D000311:Adrenal Glands / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D000924:Anticholesteremic Agents / Q000633:toxicity; D000069059:Atorvastatin; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D006538:Heptanoic Acids / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D011758:Pyrroles / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000924:Anticholesteremic Agents; D006538:Heptanoic Acids; D011758:Pyrroles; D000431:Ethanol; D000069059:Atorvastatin