VH gene segments in the mouse and human genomes.
We have examined the mouse genome sequence to determine its VH gene segment repertoire. In all, 141 segments are mapped to a 3 Mb region of chromosome 12. There is evidence that 92 of these are functional in the mouse strain used for the genome sequence, C57BL/6J; 12 are functional in other mouse strains, and 37 are pseudogenes. The mouse VH gene segment repertoire is therefore twice the size of that in humans. The mouse and human loci bear no large-scale similarity to each other. The 104 functional segments belong to one of the 15 known sequence subgroups, which have been further clustered into eight sets here. Seven of these sets, comprising 101 sequences, are related to five of the human VH families and have the same canonical structures in their hypervariable regions. Duplication of members of one set in the distal half of the locus is mainly responsible for the larger size of the mouse repertoire. Phylogenetic analysis of the VH segments indicates that most of the sequences in the human and mouse VH loci have arisen subsequent to the divergence of the two organisms from their common ancestor.
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. bdb@mrc-lmb.cam.ac.uk
Bernard
B
de Bono
Martin
M
Madera
Cyrus
C
Chothia
D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D005803:Genes, Immunoglobulin; D014644:Genetic Variation; D016678:Genome; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000145:classification / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000145:classification / Q000235:genetics*; D016013:Likelihood Functions; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016415:Sequence Alignment
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region