Duffy antigen receptor and genetic susceptibility of African Americans to acute rejection and delayed function.
BACKGROUND
The unique distribution of the alleles for the Duffy antigen receptor complex (DARC) that binds to chemokines may be associated with the rates of acute rejection and delayed allograft function (DGF) among African Americans.


METHODS
A prospective, multicenter cohort study enrolled 222 African American recipients of cadaveric renal allografts from eight adult transplant centers. Subjects were typed by allele-specific polymerase chain reaction (ASPCR) for the polymorphism at position 535 that determines the level of transcription. Associations of DARC genotypes were examined in Cox hazards models with episodes of acute rejection and in logistic regression models with the development of DGF.


RESULTS
FyB Null homozygosity was observed among 67% of the recipients. Fifteen percent of the study cohort experienced at least one episode of acute rejection, and the incidence of DGF was 42.5%. The number of FyB Null alleles and FyB Null homozygosity had no detectable association with the rate of acute rejection (P > 0.50) or with the development of DGF (P > 0.50).


CONCLUSIONS
The susceptibility of African American recipients to acute rejection and to DGF was not confirmed to be associated with DARC alleles or genotype. Future studies should exclude a potential role of donor-related DARC in transplant outcomes.
Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA. kevin.mange@pharma.novartis.com
Kevin C
KC
Mange
Eline Luning
EL
Prak
Malek
M
Kamoun
Yangzhu
Y
Du
Noah
N
Goodman
Theodore
T
Danoff
Tracey
T
Hoy
Melissa
M
Newman
Marshall M
MM
Joffe
Harold I
HI
Feldman
D000208:Acute Disease; D000328:Adult; D001741:Black or African American / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000235:genetics*; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011956:Receptors, Cell Surface / Q000235:genetics*
C473745:ACKR1 protein, human; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface