Development of a PCR-based mating-type assay for Clavicipitaceae.
This study developed a PCR-based mating-type assay for Clavicipitaceae. PCR primer sets for the mating-type genes MAT1-1-1 and MAT1-2-1 were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, Cordyceps and Epichloë). Most of the clavicipitaceous fungi possessed either MAT1-1-1 or MAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.
The Agricultural High-Tech Research Center, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. yokoyama@ccmfs.meijo-u.ac.jp
Eiji
E
Yokoyama
Kenzo
K
Yamagishi
Akira
A
Hara
D017931:DNA Primers; D004271:DNA, Fungal / Q000145:classification; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005838:Genotype; D006999:Hypocreales / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D012337:RNA, Ribosomal, 18S