Presence of SNAP-25 in rat mast cells.
Mast cells participate in inflammation and allergies by releasing active mediators stored in numerous cytoplasmic granules. Degranulation implies compound exocytosis which involves a combination of granule-granule and granule-plasma membrane fusions. One of the most important proteins in the exocytotic process in neural and endocrine cells is the synaptosomal associated protein of 25 kDa (SNAP-25). In the present study, using a highly specific monoclonal antibody against SNAP-25, we have demonstrated by immunocytochemistry, western blot and immunoelectron microscopy the presence of SNAP-25 in rat peritoneal mast cells. Likewise we localized the protein mainly on the membrane of the secretory granules. Thus while the precise function of SNAP-25 in mast cells remains to be elucidated, it may be envolved in granule-granule fusion needed in degranulation.
Dpto. de Microbiología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, CP 20100, Aguascalientes, Ags, Mexico. emsalin@correo.uaa.mx
Eva
E
Salinas
Javier
J
Ventura
Laura E
LE
Córdova
J
J
Luis Quintanar
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D008407:Mast Cells / Q000737:chemistry* / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D050825:Synaptosomal-Associated Protein 25
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25