A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin.
A hemorrhagic proteinase, jerdohagin, was purified from Trimeresurus jerdonii venom by gel filtration and ion-exchange chromatographies. It was a single chain polypeptide with an apparent molecular weight of 96 kDa as estimated by SDS-PAGE under the non-reducing and reducing conditions. Internal peptide sequencing indicated that it consisted of metalloproteinase, disintegrin-like and cysteine-rich domains and belonged to the class III snake venom metalloproteinases (class P-III SVMPs). Like other typical metalloproteinases, hemorrhagic activities of jerdohagin were completely inhibited by EDTA, but not by PMSF. Jerdohagin preferentially degraded alpha-chain of human fibrinogen. Interestingly, jerdohagin did not activate human prothrombin, whereas it cleaved human prothrombin and fragment F1 of activated human prothrombin.
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China.
Run-Qiang
RQ
Chen
Yang
Y
Jin
Jian-Bo
JB
Wu
Xing-Ding
XD
Zhou
Dong-sheng
DS
Li
Qiu-Min
QM
Lu
Wan-Yu
WY
Wang
Yu-Liang
YL
Xiong
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000378:metabolism*; D004492:Edetic Acid / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D006470:Hemorrhage / Q000139:chemically induced; D006801:Humans; D007328:Insulin / Q000378:metabolism; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011516:Prothrombin / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017841:Trimeresurus
D003435:Crotalid Venoms; D007328:Insulin; D010446:Peptide Fragments; C045879:prothrombin fragment 1.2; D011516:Prothrombin; D005340:Fibrinogen; D004492:Edetic Acid; D045726:Metalloproteases