Vitamin D receptor alleles, bone mineral density and turnover in postmenopausal osteoporotic and healthy women.
OBJECTIVE
Vitamin D receptor (VDR) gene polymorphisms and bone metabolic markers were investigated as potential genetic markers for osteoporosis in postmenopausal Turkish women. The relationship between their VDR gene polymorphisms and bone states was determined.


METHODS
Restriction fragment length polymorphisms at the VDR gene locus (i.e., for BsmI, ApaI, and TaqI) was investigated in 75 postmenopausal osteoporotic (53.16 +/- 1.31 years) and 66 healthy (52.62 +/- 1.69 years) Turkish women and the genotypes were related to bone mineral density (BMD) at femoral neck (FN), lumbar spine (L1-4), trochanter, Ward's triangle (Ward's) and metabolic parameters of bone turnover.


RESULTS
In osteoporotic women, TaqI genotype-related differences of the VDR gene were found to be significant at all BMD sites; TT genotype had higher L1-4 BMD values than Tt and tt (p < 0.05); tt genotype had significantly lower BMD at FN (p < 0.05), trochanter (p < 0.01), and Ward's (p < 0.05) compared to TT genotype. The tt genotype was found to be associated with higher (p < 0.05) serum osteocalcin levels compared to Tt and TT genotypes in the osteoporotic women, whereas no such association was found for the healthy women.


CONCLUSIONS
Our data showed an association between VDR TaqI genotype and BMD at the FN, L1-4, trochanter and Ward's triangle in nonobese postmenopausal osteoporotic women. Thus the VDR gene Taql polymorphism modulates differences in BMD in the postmenopausal osteoporotic women.
Duman
Belgin Süsleyici
BS
Department of Medical Biology and Genetics, Kadir Has University Medical Faculty, Gayrettepe-Istanbul, Turkey.
Tanakol
Refik
R
Erensoy
Nevin
N
Oztürk
Melek
M
Yilmazer
Selma
S
D015992:Body Mass Index; D015519:Bone Density / Q000235:genetics*; D016723:Bone Remodeling / Q000502:physiology*; D002118:Calcium / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D006909:Hydroxyproline / Q000652:urine; D015675:Osteocalcin / Q000097:blood; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*; D014421:Turkey
D018167:Receptors, Calcitriol; D015675:Osteocalcin; C053864:endodeoxyribonuclease BsmI; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases; D006909:Hydroxyproline; D002118:Calcium