[Alteration of protein and amino acid components and Rubisco activity in leaves of thermo-sensitive mutant line of rice during induced of green and yellow banding].
We have determined the Rubisco components and activity, whole leaf protein and amino acid components of thermo-sensitive mutant line 1103s of rice (Oryza sativa ssp. indica) leaves during induces green and yellow banding in this study. The results are as follows: The structure and components of Rubisco in the mutant are the same as in the wild form and relatively stable, but the activity of the mutant Rubisco greatly changes as a special protein of molecular weight 56.2 kD (PI=4.5) appears and disappears. When the green-yellow bands appear, the special protein disappears, and the activity of the mutant-rice Rubisco decreases, whereas when the green-yellow bands in the same part of the leaves disappear, the special protein appears and the activity of Rubisco is increase. The above shows the changes of the activity of the mutant-rice Rubisco during photosynthesis are closely related to the special protein in the leaves and its structure and components, and possibly to the regulating protein of Rubisco. The protein particularly regulates metabolic processes of amino acids preventing regulation of the preceding amino acids, such that the formation of the structural material in chloroplast is prevented, and finally the chloroplast thylakoid structure degenerates.
Institute of Botany, The Chinese Academy of Sciences, Beijing 100093.
Ji Rong
JR
Shao
Xue Mei
XM
Zhu
Rong
R
Xie
Zheng Long
ZL
Ren
Jing San
JS
Sun
D000596:Amino Acids / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*
D000596:Amino Acids; D010940:Plant Proteins; D012273:Ribulose-Bisphosphate Carboxylase