Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments.
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.
Tengs
Torstein
T
Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
LaFramboise
Thomas
T
Den
Robert B
RB
Hayes
David N
DN
Zhang
Jianhua
J
DebRoy
Saikat
S
Gentleman
Robert C
RC
O'Neill
Keith
K
Birren
Bruce
B
Meyerson
Matthew
M
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D019295:Computational Biology; D003198:Computer Simulation; D044708:DNA, Concatenated / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D015894:Genome, Human; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008390:Markov Chains; D016133:Polymerase Chain Reaction; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification
D044708:DNA, Concatenated; D015252:Deoxyribonucleases, Type II Site-Specific