Identification and molecular cloning of putative odorant-binding proteins from the American palm weevil, Rhynchophorus palmarum L.
We have identified and cloned the cDNAs encoding two odorant-binding proteins (OBPs) from the American palm weevil (APW) Rhynchophorus palmarum (Coleoptera, Curculionidae). Degenerate primers were designed from the N-terminal sequences and were used in polymerase chain reaction (PCR) in order to obtain full-length sequences in both males and females. In both sexes, two different cDNAs were obtained, encoding 123 and 115 amino acid-deduced sequences. Each sequence showed few amino acid differences between the sexes. The proteins were named RpalOBP2 and RpalOBP4 for male, RpalOBP2' and RpalOBP4' for female, with the types 2 and 4 presenting only 34% identities. These proteins shared high identity with previously described coleopteran OBPs. In native gels, RpalOBP2 clearly separated into two bands and RpalOBP4 into three bands, suggesting the presence of several conformational isomers. Thus, OBP diversity in this species may rely on both the presence of OBPs from different classes and the occurrence of isoforms for each OBP.
INRA, UR 258 Phytopharmacie et Médiateurs Chimiques, Route de Saint-Cyr, F-78026 Versailles Cedex, France. patricia.le-meillour@univ-lille1.fr
Patricia
P
Nagnan-Le Meillour
Marie-Christine
MC
François
Emmanuelle
E
Jacquin-Joly
D000569:Americas; D000818:Animals; D003001:Cloning, Molecular; D001517:Coleoptera / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018035:Receptors, Odorant; C045648:odorant-binding protein