[Gene expression profile in early differentiation of K562 cells induced by matrine].
OBJECTIVE
To compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.


METHODS
K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.


RESULTS
From 8465 screened genes, 30 differentially expressed genes were found. Among them 18 showed decreased expression including oncogene and proto-oncogene, signal transduction gene, DNA binding and transcription gene etc, 12 showed increased expression including cell receptor gene, immune-associated gene, metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell.


CONCLUSIONS
The genes, that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.
Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, China.
Yan
Y
Zhang
Ling-Di
LD
Ma
Yu-Juan
YJ
He
Ji-Kai
JK
Jiang
D000470:Alkaloids / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D000090063:Proto-Oncogene Mas; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
D000470:Alkaloids; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011807:Quinolizines; D000093842:Matrines