Individuals' leukocyte DNA double-strand break repair as an indicator of radiosurgery responses for cerebral arteriovenous malformations.
To evaluate the feasibility of using radiosensitivity of peripheral leukocytes as a predictor of clinical therapeutic responses to radiosurgery in individuals with cerebral arteriovenous malformation (AVM), we enrolled 18 patients years after they had received Gamma Knife radiosurgery for their cerebral AVM. The AVMs were shown with different degrees of regression in size in posttherapeutic periods. The peripheral leukocytes of these patients were collected at the last neuroimaging follow-ups. The leukocytes, before and 1 and 2 h after 8 Gy external gamma-irradiation, were evaluated for the amounts of DNA double-strand breaks (DSB) in 50 randomly selected individual nuclei by the neutral single cell gel electrophoresis, or so-called comet analysis. After being adjusted for gender and age at radiosurgery, the individuals with less posttherapeutic regression in AMV sizes or relatively poor or inadequate responses to radiosurgery were shown to have significantly higher DSB repair capacity on their leukocytes by comet analysis. These results suggested that in vitro radiosensitivity of peripheral leukocytes may provide valuable information for predicting therapeutic response or for adjusting irradiation doses in AVM radiosurgery.
Department of Radiology, Taipei Veterans General Hospital, Taiwan.
Wan-Yuo
WY
Guo
Hung-Chi
HC
Pan
Hsiu-Mei
HM
Wu
Wanhwa A
WA
Hsieh
Mong-Hsun
MH
Tsai
Yau-Mei
YM
Chow
Wen-Yu
WY
Chung
Cheng-Ying
CY
Shiau
Shin-Kuang
SK
Chen
Wushou P
WP
Chang
D000293:Adolescent; D000328:Adult; D002648:Child; D004247:DNA / Q000528:radiation effects / Q000648:ultrastructure; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects*; D005240:Feasibility Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D002538:Intracranial Arteriovenous Malformations / Q000175:diagnosis* / Q000473:pathology / Q000532:radiotherapy*; D007962:Leukocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D017063:Outcome Assessment, Health Care / Q000379:methods*; D016634:Radiosurgery / Q000379:methods*; D013223:Statistics as Topic; D016896:Treatment Outcome
D004247:DNA