[Effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain].
The effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied. After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days, the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain. The results showed that Cu2+ and Cu-EDTA complex can induce the expression of HSP70 significantly. Comparing with the test groups exposed to Cu2+, the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal. It was found that the fish exposed to the concentration of Cu2+ which below the national fishery water quality standard of 0.01 mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment Science, Nanjing University, Nanjing 210093, China.
Hua
H
Shen
Xiao-rong
XR
Wang
Jing-fei
JF
Zhang
Hui
H
Liu
Yong-juan
YJ
Zhao
D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D003300:Copper; D004492:Edetic Acid