Interlaboratory comparison trial on cylindrospermopsin measurement.
The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.
Department of Hygienic Water Biology, National Institute of Environmental Health, P.O. Box 26, Budapest H-1450, Hungary. toroka@okk.antsz.hu
Andrea
A
Törökné
Maria
M
Asztalos
Maria
M
Bánkiné
Heike
H
Bickel
Georg
G
Borbély
Shmuel
S
Carmeli
Geoffrey A
GA
Codd
Jutta
J
Fastner
Qiong
Q
Huang
Andrew
A
Humpage
James S
JS
Metcalf
Erzsebet
E
Rábai
Assaf
A
Sukenik
Gyula
G
Surányi
Gabor
G
Vasas
Viktor
V
Weiszfeiler
D000470:Alkaloids; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D000087522:Cyanobacteria Toxins; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil