Histopathological analysis in experimental macular surgery with trypan blue.
OBJECTIVE
To analyse the effect of trypan blue on the retina in an experimental setting of macular surgery.


METHODS
Porcine eyes were used within 3 hours after death. The eyes were hemisected and the vitreous removed. Trypan blue (0.15%) was applied over the trephined posterior pole, whereas the rest of the eye cup was filled with a balanced salt solution (BSS). The dye and the BSS were removed after 1 minute and the complete eye cup irrigated and filled with fresh BSS. Both the treated and untreated retinas were illuminated with a standard surgical light pipe and source at maximum power for 10 minutes. Both the trypan blue exposed retina and the non-treated surrounding retina were processed for histology.


RESULTS
Exposure of the retina to trypan blue for 1 minute, followed by illumination caused no histologically detectable damage compared to the controls. No microarchitectural disorganisation, cellular disruption, or affection of the vitreoretinal interface was detected.


CONCLUSIONS
These findings indicate that a 1 minute exposure of trypan blue followed by illumination does not cause an acute morphologically detectable toxic effect on the porcine retina.
Department of Ophthalmology I, Eberhard-Karls University of Tuebingen, Schleichstrasse 12-15, 72076 Tuebingen, Germany. salvatore.grisanti@med.uni-tuebingen.de
S
S
Grisanti
P
P
Szurman
O
O
Tatar
F
F
Gelisken
S
S
Aisenbrey
J
J
Oficjalska-Mlynczak
R
R
Kaczmarek
K U
KU
Bartz-Schmidt
D000818:Animals; D004396:Coloring Agents / Q000009:adverse effects*; D004353:Drug Evaluation, Preclinical; D006863:Hydrogen-Ion Concentration; D008266:Macula Lutea / Q000601:surgery*; D012160:Retina / Q000187:drug effects* / Q000473:pathology; D013552:Swine; D014343:Trypan Blue / Q000009:adverse effects*
D004396:Coloring Agents; D014343:Trypan Blue