The Sac3 homologue shd1 is involved in mitotic progression in mammalian cells.
Saccharomyces Sac3 required for actin assembly was shown to be involved in DNA replication. Here, we studied the function of a mammalian homologue SHD1 in cell cycle progression. SHD1 is localized on centrosomes at interphase and at spindle poles and mitotic spindles, similar to alpha-tubulin, at M phase. RNA interference suppression of endogenous shd1 caused defects in centrosome duplication and spindle formation displaying cells with a single apparent centrosome and down-regulated Mad2 expression, generating increased micronuclei. Conversely, increased expression of SHD1 by DNA transfection with shd1-green fluorescent protein (gfp) vector for a fusion protein of SHD1 and GFP caused abnormalities in centrosome duplication displaying cells with multiple centrosomes and deregulated spindle assembly with up-regulated Mad2 expression until anaphase, generating polyploidy cells. These results demonstrated that shd1 is involved in cell cycle progression, in particular centrosome duplication and a spindle assembly checkpoint function.
Departments of Immunology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Sefat-E-
SE
Khuda
Mikoto
M
Yoshida
Yan
Y
Xing
Tatsuya
T
Shimasaki
Motohiro
M
Takeya
Kazuhiko
K
Kuwahara
Nobuo
N
Sakaguchi
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000502:physiology; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D064246:Mad2 Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D012097:Repressor Proteins; D014162:Transfection
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C577697:MAD2L1 protein, human; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins