A new laccase from dried fruiting bodies of the monkey head mushroom Hericium erinaceum.
A laccase with a novel N-terminal sequence, a molecular mass of 63kDa, and inhibitory activity toward HIV-1 reverse transcriptase (IC(50)=9.5microM) was isolated from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. A chromatographic procedure involving ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose and fast protein liquid chromatography-gel filtration on Superdex 75 was employed. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose but unadsorbed on CM-cellulose. High activity of the enzyme was observed at pH 3-5 and at 50-80 degrees C. Its activity was completely abolished at pH 8 and 9 and after boiling for 10min. A temperature of 50 degrees C and a pH of 5.0 were optimal for its activity.
Wang
H X
HX
Department of Microbiology, China Agricultural University, Beijing, China.
Ng
T B
TB
D000363:Agaricales / Q000737:chemistry / Q000201:enzymology*; D000595:Amino Acid Sequence; D003890:Desiccation / Q000379:methods; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011489:Protein Denaturation; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013696:Temperature
D042845:Laccase