[The effects of DNA damage induced by acetaldehyde].
The effects of DNA damage induced by the typical environmental pollutant acetaldehyde were studied with single cell gel electrophoresis (SCGE) and high performance liquid chromatography with electrochemical detection (HPLC-EC). The results showed that acetaldehyde not only could cause DNA strand breakage but also DNA-DNA, DNA-protein crosslinks of lymphocytes of human peripheral blood. The reaction of acetaldehyde with DNA in vitro was weak, but the oxidative ability was enhanced and the reaction could produce a number of 8-OHdG adducts mediated by the Fe2+. The animal experiment shows that acetaldehyde can cause the oxidative DNA damage of rat lung tissues, which suggests that acetaldehyde have the potential genotoxicity and its chemical mechanism is relative to the crosslinks and oxidation with DNA.
Xi
Zhu-ge
ZG
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Chao
Fu-huan
FH
Yang
Dan-feng
DF
Sun
Yong-mei
YM
Li
Guan-xian
GX
Zhang
Hua-shan
HS
Zhang
Wei
W
Yang
Yu-hua
YH
Liu
Huan-liang
HL
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000079:Acetaldehyde / Q000737:chemistry / Q000633:toxicity*; D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D004785:Environmental Pollutants / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051381:Rats
D004785:Environmental Pollutants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000079:Acetaldehyde