Noninvasive histologic grading of solid astrocytomas using proton magnetic resonance spectroscopy.
BACKGROUND
Proton magnetic resonance spectroscopy (1H MRS) constitutes a promising modality to assess intracranial pathology. We present our experience using this method in grading solid brain astrocytomas.


METHODS
Using a 1.5-Tesla MRI unit, 71 patients with the radiographic diagnosis of astrocytoma were examined. Water-suppressed single-voxel 1H MRS was employed in all of our patients. The concentrations of choline (Cho), N-acetyl-aspartate (NAA), phosphocreatine-creatine (Pcr-Cr), myo-inositol (MI), lactate (Lac), lipids (Lip) as well as the metabolite ratios of Cho/Pcr-Cr, NAA/PCr-Cr and NAA/Cho were calculated. An appropriate surgical biopsy was performed. Standard pathology examination was employed in a double-blinded fashion.


RESULTS
An increased concentration of Cho and decreased concentrations of Pcr-Cr and NAA were detected. The concentrations of Lac, Lip and MI varied inconsistently, even among tumors of the same histologic grade. The Cho/Pcr-Cr ratio was calculated. This ratio was found to be 2.15 +/- 0.26 in 27 patients with astrocytomas grade I and II, 2.78 +/- 0.09 in 18 patients with grade III, and 5.40 +/- 0.16 in 26 patients with grade IV.


CONCLUSIONS
The increased concentration of Cho is due to the increased cellularity and a relatively increased number of membranous structures in highly malignant tumors. In abnormal anaerobic metabolic tumor states there is relatively less phosphorylization of creatine. By using the Cho/Pcr-Cr ratio the concomitant effects of structural and metabolic alteration can thereby be emphasized for diagnostic advantage.


CONCLUSIONS
The Cho/Pcr-Cr is a very important and statistically significant marker (p = 0.043) determining the degree of intracranial astrocytoma malignancy.
Department of Neurosurgery, Medical Center of Central Georgia, Mercer University School of Medicine, Macon, USA. knfountasmd@excite.com
Kostas N
KN
Fountas
Effie Z
EZ
Kapsalaki
Robert L
RL
Vogel
Ioannis
I
Fezoulidis
Joe Sam
JS
Robinson
Efstathios D
ED
Gotsis
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001254:Astrocytoma / Q000145:classification / Q000175:diagnosis* / Q000378:metabolism; D001932:Brain Neoplasms / Q000145:classification / Q000175:diagnosis* / Q000378:metabolism; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011522:Protons
D011522:Protons