Effects of ingesting Lactobacillus- and Bifidobacterium-containing yogurt in subjects with colonized Helicobacter pylori.
BACKGROUND
Evidence suggests that ingesting lactic acid bacteria exerts a suppressive effect on Helicobacter pylori infection in both animals and humans. Supplementing with Lactobacillus- and Bifidobacterium-containing yogurt (AB-yogurt) was shown to improve the rates of eradication of H. pylori in humans.


OBJECTIVE
We administered AB-yogurt to subjects with asymptomatic H. pylori to test whether the yogurt could inhibit H. pylori growth.


METHODS
The in vitro inhibition of H. pylori growth was determined by inoculating Lactobacillus acidophilus La5 or Bifidobacterium lactis Bb12 on plates that were inoculated with H. pylori. Assessment of the viability of H. pylori was performed by the mixed culture method with La5 or Bb12. In an intervention study, 59 adult volunteers infected with H. pylori were given AB-yogurt (10(7) colony-forming units of both La5 and Bb12/mL) twice daily after a meal for 6 wk. Eleven subjects positive for H. pylori infection were treated with milk placebo as control subjects. H. pylori bacterial loads were determined with use of the (13)C-urea breath test, which was performed before and 4 and 8 wk after the start of AB-yogurt supplementation.


RESULTS
Bb12 exerted an in vitro inhibitory effect against H. pylori, whereas La5 did not show an effect. Administration of AB-yogurt decreased the urease activity of H. pylori after 6 wk of therapy (P < 0.0001).


CONCLUSIONS
Regular intake of yogurt containing Bb12 and La5 effectively suppressed H. pylori infection in humans.
Wang
Kuan-Yuan
KY
Department of Internal Medicine, Kaohsiung Municipal United Hospital, Kaohsiung, Taiwan, Republic of China.
Li
Shui-Nin
SN
Liu
Chiang-Shin
CS
Perng
Daw-Shyong
DS
Su
Yu-Chung
YC
Wu
Deng-Chyang
DC
Jan
Chang-Ming
CM
Lai
Chun-Huang
CH
Wang
Tsu-Nai
TN
Wang
Wen-Ming
WM
D000328:Adult; D000898:Antibiosis; D001644:Bifidobacterium / Q000502:physiology*; D001944:Breath Tests; D002247:Carbon Isotopes; D015169:Colony Count, Microbial; D005260:Female; D005516:Food Microbiology; D016481:Helicobacter Infections / Q000382:microbiology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000254:growth & development*; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D007779:Lactobacillus acidophilus / Q000502:physiology; D008297:Male; D008875:Middle Aged; D019936:Probiotics; D016896:Treatment Outcome; D015014:Yogurt / Q000382:microbiology*
D002247:Carbon Isotopes