Studies on alkaline protease produced by Bacillus sp. NG312.
An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at pH 11.0 and 60 degrees C. The temperature optimum was increased by 10 degrees C in presence of Ca2+. The crude enzyme was found to have half-life of 11 d at 37 degrees C and maximum stability at pH 9.0-10.0. It also exhibited very good stability in presence of detergent components and some locally available commercial detergent powders.
Institute of Microbial Technology, Sector 39-A, Chandigarh-160 036, India.
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