[Microstructure of tympanic membrane in chronic otitis media: immunohistochemical evaluation].
Chronic otitis media (COM) is an inflammatory process involving the middle ear mucosa and the tympanic membrane. The healing and epidermization is mostly impaired by immunological response of the host. Investigating the activity and the function of immunological response elements one can learn the immunological mechanisms taking place in chronic otitis media. The ultrastructural investigations of the tympanic membrane were done on its fragments obtained from 19 patients with COM during middle ear surgery, performed at ENT Department of Medical University of Gdańsk in the years 1997-1999. Immunohistochemical investigations were performed using monoclonal antibodies against tenascin, S-100 protein, Ki 67, CD 31, F VIII, HLA-DR, TGFbeta1 and EGFR. The control group was 11 healthy tympanic membranes from cadavers. The presence of tenascin was proven in all COM tympanic membranes and in 45.5% of those from control group. S-100 protein was present in 88.9% of the patients with COM and absent in control group. Ki 67 was observed in 44.4% of the patients with COM and in 27.3% of the healthy tympanic membranes. Angiogenesis factors (CD 31 and FVIII) were present in 77.8% of the investigated COM tympanic membranes, in control group in 45.5%. HLA-DR expression was observed in 90% COM patients, in control group in 72.7%. Growth factor TGFbeta1 was present in the all cases in mucous and fibrous layer and in 54.5% of healthy tympanic membranes. EGF receptor was present in 60% of COM patients, mainly in epithelial layer of tympanic membrane and in 54.5% of those from control group. The presented investigations confirm the immunological activity of tympanic membrane in chronic otitis media.
Katedra i Klinika Chorób Uszu, Nosa, Gardła i Krtani AM w Gdańsku. jerzyk@amedec.amg.gda.pl
Jerzy
J
Kuczkowski
Alicja
A
Bakowska
Bogusław
B
Mikaszewski
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D016022:Case-Control Studies; D002908:Chronic Disease; D066246:ErbB Receptors / Q000032:analysis; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010033:Otitis Media / Q000473:pathology* / Q000601:surgery; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011044:Poland; D009418:S100 Proteins / Q000032:analysis; D019063:Tenascin / Q000032:analysis; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis; D014432:Tympanic Membrane / Q000737:chemistry / Q000601:surgery / Q000648:ultrastructure*; D014841:von Willebrand Factor / Q000032:analysis
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D009418:S100 Proteins; D019063:Tenascin; D016212:Transforming Growth Factor beta; D014841:von Willebrand Factor; D066246:ErbB Receptors