Effect of dietary fatty acids on the postprandial fatty acid composition of triacylglycerol-rich lipoproteins in healthy male subjects.
OBJECTIVE
The aim of the present study was to investigate the effect of trans-18:1 isomers compared to other fatty acids, especially saturates, on the postprandial fatty acid composition of triacylglycerols (TAG) in chylomicrons and VLDL.


METHODS
A randomised crossover experiment where five interesterified test fats with equal amounts of palmitic acid (P fat), stearic acid (S fat), trans-18:1 isomers (T fat), oleic acid (O fat), or linoleic acid (L fat) were tested.


METHODS
A total of 16 healthy, normolipidaemic males (age 23+/-2 y) were recruited.


METHODS
The participants ingested fat-rich test meals (1 g fat per kg body weight) and the fatty acid profiles of chylomicron and VLDL TAG were followed for 8 h.


RESULTS
The postprandial fatty acid composition of chylomicron TAG resembled that of the ingested fats. The fatty acids in chylomicron TAG were randomly distributed among the three positions in accordance with the distributions in test fats. Calculations of postprandial TAG concentrations from fatty acid data revealed increasing amounts up to 4 h but lower response curves (IAUC) for the two saturated fats in accordance with previous published data. The T fat gave results comparable to the O and L fats. The test fatty acids were much less reflected in VLDL TAG and there was no dietary influence on the response curves.


CONCLUSIONS
The fatty acid composition in the test fats as well as the positional distributions of these were maintained in the chylomicrons. No specific clearing of chylomicron TAG was observed in relation to time.
Bysted
A
A
BioCentrum-DTU, Section of Biochemistry and Nutrition, Center of Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark. aby@dfvf.dk
Hølmer
G
G
Lund
P
P
Sandström
B
B
Tholstrup
T
T
D000328:Adult; D019540:Area Under Curve; D002914:Chylomicrons / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007536:Isomerism; D008079:Lipoproteins, VLDL / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019518:Postprandial Period / Q000502:physiology; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism
D002914:Chylomicrons; D004041:Dietary Fats; D005227:Fatty Acids; D008079:Lipoproteins, VLDL; D014280:Triglycerides