Effect of feeding on peripheral circadian rhythms and behaviour in mammals.
Although feeding time is a dominant cue for circadian rhythms in mammalian peripheral tissue, the effect of feeding and fasting on circadian gene expression and behaviour is unknown. Here we report that fasting does not affect the phase of rhythmic mRNA expression levels of the clock genes, mPer1, mPer2 and of the clock controlled gene, mDBP. However, the levels of each of these genes were significantly altered in different ways and recovered by feeding. We also found that feeding enhances phase-shifting to a new light-dark cycle of rhythmic mPer2 mRNA expression in the heart. Furthermore, feeding enhances the phase-shifting to new light-dark cycle of behaviour more than fasting. Our data indicate that feeding is an important cue for circadian behaviour rhythms as well as for the photo-entrainment of peripheral clock gene expression.
Kobayashi
Hisato
H
Clock Cell Biology, Institute of Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Oishi
Katsutaka
K
Hanai
Shuji
S
Ishida
Norio
N
D000818:Animals; D056926:CLOCK Proteins; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D003624:Darkness; D004435:Eating / Q000235:genetics / Q000502:physiology*; D005215:Fasting; D005247:Feeding Behavior; D005786:Gene Expression Regulation; D008027:Light; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D056926:CLOCK Proteins; C538944:Clock protein, mouse