Prothymosin alpha is localized in mitotic spindle during mitosis.
Prothymosin a is a small, acidic, ubiquitous protein, thought to play a role in cell proliferation, carcinogenesis and apoptosis. We have reported earlier that in the interphase nucleus prothymosin a exhibits a punctuated nuclear distribution associated with transcription sites. Moreover, the protein was found to localize in 1-6 subnuclear domains where PML and CstF64 proteins were also identified. In the present study we followed the subcellular distribution of prothymosin a during mitosis. Our data identify prothymosin a to colocalize with alpha-tubulin in the mitotic spindle throughout mitosis, and to decorate the midbody during cytokinesis. Moreover nocodazole treatment disrupted prothymosin alpha and alpha-tubulin colocalization at the centrosomes of the interphase cell. Prothymosin a was also found to decorate gamma-tubulin identified centrosomes, during mitosis. Taken together our colocalization study suggests involvement of prothymosin a, in the formation, maintenance, or functioning of the mitotic spindle.
Laboratory of Biological Chemistry, University of loannina Medical School, GR-451 10 loannina, Greece. kvareli@cc.uoi.gr
Katerina
K
Vareli
Maria
M
Frangou-Lazaridis
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D013947:Thymosin / Q000031:analogs & derivatives* / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000970:Antineoplastic Agents; D011498:Protein Precursors; D014404:Tubulin; C040782:prothymosin alpha; D013947:Thymosin; D015739:Nocodazole