Lithospermic acid B isolated from Salvia miltiorrhiza ameliorates ischemia/reperfusion-induced renal injury in rats.
The present study was designed to examine whether lithospermic acid B (LSB) isolated from Salvia miltiorrhiza has an ameliorative effect on renal functional parameters in association with the expression of aquaporin 2 (AQP 2) and Na,K-ATPase in the ischemia-reperfusion induced acute renal failure (ARF) rats. LSB showed strong antioxidant activity against production of reactive oxygen species (ROS), ROS-induced hemolysis, and production of lipid peroxide in a dose-dependent manner. Polyuria caused by down-regulation of renal AQP 2 in the ischemia-reperfusion induced ARF rats was partially restored by administration of LSB (40 mg/kg, i.p.), restoring expression of AQP 2, in renal inner and outer medulla. The expression of Na,K-ATPase alpha1 subunit in outer medulla of the ARF rats was also restored in the ARF rats by administration of LSB, while beta1 subunit level was not altered. The renal functional parameters including creatinine clearance, urinary sodium excretion, urinary osmolality, and solute-free reabsorption were also partially restored in ischemia-ARF rats by administration of LSB. Histological study also showed that renal damages in the ARF rats were abrogated by administration of LSB. Taken together, these data indicate that LSB ameliorates renal defects in rats with ischemia-reperfusion induced ARF, most likely via scavenging of ROS.
Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea, South Korea.
Dae Gill
DG
Kang
Hyuncheol
H
Oh
Eun Jin
EJ
Sohn
Tae Young
TY
Hur
Kang Chang
KC
Lee
Kwang Jin
KJ
Kim
Tai Yo
TY
Kim
Ho Sub
HS
Lee
D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D000818:Animals; D051399:Aquaporin 2; D020346:Aquaporins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D053630:Depsides; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016877:Oxidants / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013481:Superoxides / Q000378:metabolism
C496025:Aqp2 protein, rat; D051399:Aquaporin 2; D020346:Aquaporins; D001572:Benzofurans; D053630:Depsides; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D016877:Oxidants; D011506:Proteins; D013481:Superoxides; D017665:Hydroxyl Radical; C076944:salvianolic acid B; D000254:Sodium-Potassium-Exchanging ATPase