Acute impairment of hepatic microcirculation and recruitment of nonparenchymal cells by intrasplenic hepatocyte transplantation.
OBJECTIVE
Over the last 20 years, hepatocyte transplantation (HcTx) has advanced from the experimental to the clinical stage. To date, HcTx has been performed in 30 patients in the United States. Regardless whether hepatocytes are transplanted into the spleen and migrate to the liver or are injected directly into the portal vein, transplanted liver cells will, to some extent, congest the recipient liver microcirculation. The potential negative consequences of intrasplenic HcTx were the subject of this study.


METHODS
By using intravital microscopy, the authors investigated whether intrasplenic HcTx of 20 x 10(6) allogenic hepatocytes would influence liver perfusion, excretory liver function, and nonparenchymal cells (Kupffer and Ito cells) in vivo.


RESULTS
The sinusoidal perfusion rate declined significantly from 94% (control) to 84% on day 1 and 76% on day 7. Bile acid excretion decreased in a similar fashion from 0.924 mg/h (control) to 0.669 mg/h on day 7. The authors observed a significant increase of Ito cells from 81.1 cells per microscopic field (control) to 97.1 (day 1) and an increase of Kupffer cells (KC; 6.1 cells per microscopic field on day 1 v 3.8 on control).


CONCLUSIONS
This study shows an acute impairment of hepatic microcirculation and hepatucellular function along with an recruitment and activation of nonparenchymal cells in the early posttransplantation period after intrasplenic HcTx. Kupffer cell recruitment indicates an activation of local host defense, and Ito cell activation implies the initiation of liver repair mechanisms owing to ischemia-related cell damage.
Wilhelm
A
A
Department of General Surgery, Georg-August-University, Goettingen, Germany.
Leister
I
I
Sabandal
P
P
Krause
P
P
Becker
H
H
Markus
P M
PM
D000818:Animals; D001646:Bile / Q000378:metabolism; D002465:Cell Movement; D017690:Cell Transplantation / Q000009:adverse effects; D022781:Hepatocytes / Q000637:transplantation*; D007728:Kupffer Cells / Q000502:physiology; D008099:Liver / Q000473:pathology; D008102:Liver Circulation; D008297:Male; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen; D016042:Transplantation, Heterotopic / Q000009:adverse effects*; D014184:Transplantation, Homologous