Antibody detection tests for Onchocerca volvulus: comparison of the sensitivity of a cocktail of recombinant antigens used in the indirect enzyme-linked immunosorbent assay with a rapid-format antibody card test.
There is a need for a specific, sensitive, and practical diagnostic test to monitor onchocerciasis elimination campaigns. In April 2001, an enzyme-linked immunosorbent assay (ELISA) using 3 recombinant antigens and a rapid-format antibody card test (immunochromatographic test; ICT) using an individual antigen were compared in a Mexican population with onchocerciasis. The sensitivity of the ELISA and ICT was 97% and 86%, respectively.
Rodríguez-Pérez
Mario A
MA
Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Boulevard del Maestro esquina Elías Piña, Reynosa, Tamaulipas, Mexico. mar@mail.cbg.ipn.mx
Domínguez-Vázquez
Alfredo
A
Méndez-Galván
Jorge
J
Sifuentes-Rincón
Ana M
AM
Larralde-Corona
Patricia
P
Barrera-Saldaña
Hugo A
HA
Bradley
Janette E
JE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017181:Onchocerca volvulus / Q000276:immunology / Q000302:isolation & purification*; D009855:Onchocerciasis / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000947:Antigens, Helminth