Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63.
Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.
Habe
Hiroshi
H
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. uhhabe@mail.ecc.u-tokyo.ac.jp
Chung
Jin-Sung
JS
Kato
Hiroyuki
H
Ayabe
Yuko
Y
Kasuga
Kano
K
Yoshida
Takako
T
Nojiri
Hideaki
H
Yamane
Hisakazu
H
Omori
Toshio
T
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005449:Fluorenes / Q000378:metabolism*; D023061:Gene Order; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010795:Phthalic Acids / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D002396:Catechols; D004269:DNA, Bacterial; D005449:Fluorenes; D010795:Phthalic Acids; C454813:2,3-dihydroxybiphenyl; C081458:1,1a-dihydroxy-1-hydrofluoren-9-one; C041509:fluorene; C032279:phthalic acid; C028401:9-fluorenone; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D010105:Oxygenases; C439587:extradiol dioxygenase; D006867:Hydrolases