The LEI/L-DNase II pathway is activated in light-induced retinal degeneration in rats.
Retinal death induced by light seems to be a caspase-independent process. In this work we investigate the LEI/L-DNase II pathway, a caspase-independent pathway, in light-induced retinal degeneration in Fischer rats. Measurement of DNase activity in total retinal extracts of light exposed Fischer rats was performed by analysing a plasmid degradation on an agarose gel. The same method was used to measure the in vitro activity of recombinant LEI (reticulocyte lysate) after incubation with calpains. L-DNase II activity is observed in retinal extracts of light exposed Fischer rats and increases with time illumination. In this apoptotic death, the activation of calpains has been shown. Here we show that L-DNase II activation is not catalized by calpains. The present study indicates that the LEI/L-DNase II may be a possible pathway activated during photoreceptor apoptosis in light-induced retinal degeneration but that this pathway is not directly activated by calpains.
INSERM U598, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
Sabine
S
Chahory
Laura
L
Padron
Yves
Y
Courtois
Alicia
A
Torriglia
D000818:Animals; D017305:Annexin A1 / Q000494:pharmacology; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D004347:Drug Interactions; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D066298:In Vitro Techniques; D008027:Light / Q000009:adverse effects*; D008297:Male; D008840:Microfilament Proteins; D010196:Pancreatic Elastase / Q000494:pharmacology; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D012162:Retinal Degeneration / Q000201:enzymology* / Q000209:etiology / Q000378:metabolism; D013997:Time Factors; D000096985:Calponins
D017305:Annexin A1; D002135:Calcium-Binding Proteins; D004706:Endodeoxyribonucleases; D008840:Microfilament Proteins; D010196:Pancreatic Elastase; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D000096985:Calponins; C019329:deoxyribonuclease II