Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities.
Curculin from Curculigo latifolia is a unique sweet protein that exhibits both sweet-tasting and taste-modifying activities. We isolated a gene that encodes a novel protein highly homologous to curculin. Using cDNAs of the previously known curculin (designated as curculin1) and the novel curculin isoform (curculin2), we produced a panel of homodimeric and heterodimeric recombinant curculins by Escherichia coli expression systems. It was revealed that sweet-tasting and taste-modifying activities were exhibited solely by the heterodimer of curculin1 and curculin2.
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
Maiko
M
Suzuki
Eiji
E
Kurimoto
Satoru
S
Nirasawa
Yutaka
Y
Masuda
Kouichi
K
Hori
Yoshie
Y
Kurihara
Nobuhisa
N
Shimba
Misako
M
Kawai
Ei-Ichiro
E
Suzuki
Koichi
K
Kato
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D031408:Curculigo / Q000737:chemistry* / Q000235:genetics*; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D013549:Sweetening Agents / Q000737:chemistry* / Q000494:pharmacology*; D013649:Taste / Q000187:drug effects* / Q000502:physiology
D004220:Disulfides; D010940:Plant Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D013549:Sweetening Agents; C065630:curculin