Agarose-coated anion exchanger prevents cell-adsorbent interactions.
A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent.
Viloria-Cols
Maria E
ME
Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Hatti-Kaul
Rajni
R
Mattiasson
Bo
B
D000327:Adsorption; D000837:Anion Exchange Resins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D007780:Lacticaseibacillus casei / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*
D000837:Anion Exchange Resins; D012685:Sepharose