A comprehensive characterization of the peptide and protein constituents of human seminal fluid.
BACKGROUND
Knowledge of the peptide and protein components of seminal fluid and their role in prostate diseases including benign prostatic hyperplasia and prostate carcinoma is scant. We have undertaken a proteomic analysis of semen as a forerunner to identifying sensitive and specific diagnostic markers of prostatic diseases; to aid in improved therapeutic intervention; and, to enhance our understanding of prostate health and disease.


METHODS
Peptide and protein components of pooled human seminal fluid (n = 5) were separated by gel electrophoresis (1D and 2D) and identified by either matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) or capillary liquid chromatography tandem mass spectrometry (LC-MS/MS).


RESULTS
Analysis by two-dimensional electrophoresis (2DE) established that there were multiple post-translational variants of the majority of the proteins. Hormones, growth factors and bioactive peptides were detected and identified.


CONCLUSIONS
We have identified over 100 protein and peptide components of normal human seminal fluid.
Fung
Kim Y C
KY
Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, Colorado, USA.
Glode
L Michael
LM
Green
Spencer
S
Duncan
Mark W
MW
D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*
D010455:Peptides; D011506:Proteins