Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues.
Dentin matrix protein 1 (DMP1) is an Arg-Gly-Asp-containing acidic phosphoprotein that was originally identified from a rat incisor cDNA library and was thought to be a dentin-specific protein. DMP1 was later shown to express in a number of hard tissue-forming cells, including osteoblasts, osteocytes, ameloblasts, and cementoblasts, and was considered to play important roles in mineralization. Further, DMP1 gene expression was also detected in fetal bovine brain and in newborn mouse brain. These findings indicate the possibility of DMP1 expression in other soft tissues. In the present study, to clarify the significance of DMP1 expression in nonmineralized tissues, we made a specific antibody to mouse DMP1 peptides and demonstrated that DMP1 protein was localized in mouse brain, pancreas, and kidney by immunohistochemistry. Further DMP1 mRNA was detected in nonmineralized mouse tissues including liver, muscle, brain, pancreas, and kidney by RT-PCR. Based on the evidence that the localization and the expression of DMP1 are not restricted to mineralized tissues, we assume that DMP1 may have functions other than the regulation of mineralization.
Terasawa
Makiko
M
Developmental Oral Health Science, Department of Orofacial Development and Function, Division of Oral Health Science, Tokyo Medical and Dental University, Tokyo, Japan.
Shimokawa
Reiko
R
Terashima
Tatsuo
T
Ohya
Keiichi
K
Takagi
Yuzo
Y
Shimokawa
Hitoyata
H
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002113:Calcification, Physiologic; D002417:Cattle; D003429:Cross Reactions; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014083:Tooth Germ / Q000276:immunology / Q000378:metabolism
C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins