Non-enzymatic platelet-activating factor formation by acetylated proteins.
Substantial amounts of platelet-activating factor (PAF 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine), the potent phospholipid mediator of allergic and inflammatory reactions, are formed upon incubation of acetylated low-density lipoprotein, acetylated bovine serum albumin (BSA) and acetylated apolipoprotein A-I with 1-0-hexadecyl-sn-glycero-3-phosphocholine (lyso-PAF). Acetylated BSA produced 0.3 nmol PAF/mg of protein after a 6 h incubation period with 40 microM lyso-PAF. The transfer of acetate bound to acetylated proteins to lyso-PAF was non-enzymatic. Chemical PAF formation by acetylated proteins, involved in lipid metabolism and transport, could lead to complication of inflammatory and allergic events.
Thanou
Paraskevi G
PG
Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina 45110, Greece.
Tsoukatos
Demokritos C
DC
D000107:Acetylation; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry; D001792:Blood Platelets / Q000737:chemistry; D002413:Cations, Divalent; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000737:chemistry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013696:Temperature
D016632:Apolipoprotein A-I; D002413:Cations, Divalent; D008077:Lipoproteins, LDL; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D012710:Serum Albumin, Bovine