Prothymosin alpha is localized in mitotic spindle during mitosis.
Prothymosin a is a small, acidic, ubiquitous protein, thought to play a role in cell proliferation, carcinogenesis and apoptosis. We have reported earlier that in the interphase nucleus prothymosin a exhibits a punctuated nuclear distribution associated with transcription sites. Moreover, the protein was found to localize in 1-6 subnuclear domains where PML and CstF64 proteins were also identified. In the present study we followed the subcellular distribution of prothymosin a during mitosis. Our data identify prothymosin a to colocalize with alpha-tubulin in the mitotic spindle throughout mitosis, and to decorate the midbody during cytokinesis. Moreover nocodazole treatment disrupted prothymosin alpha and alpha-tubulin colocalization at the centrosomes of the interphase cell. Prothymosin a was also found to decorate gamma-tubulin identified centrosomes, during mitosis. Taken together our colocalization study suggests involvement of prothymosin a, in the formation, maintenance, or functioning of the mitotic spindle.
Vareli
Katerina
K
Laboratory of Biological Chemistry, University of loannina Medical School, GR-451 10 loannina, Greece. kvareli@cc.uoi.gr
Frangou-Lazaridis
Maria
M
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D013947:Thymosin / Q000031:analogs & derivatives* / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000970:Antineoplastic Agents; D011498:Protein Precursors; D014404:Tubulin; C040782:prothymosin alpha; D013947:Thymosin; D015739:Nocodazole