Development of a accelerated solvent extraction and gel permeation chromatography analytical method for measuring persistent organohalogen compounds in adipose and organ tissue analysis.
A new analytical method has been developed for the quantification of 59 different persistent organohalogen compounds, such as polybrominated diphenyl ethers (PBDEs), polychlorinated naphthalenes (PCNs), polychlorinated biphenyls (PCBs), PCB metabolites, organochlorine pesticides (OCPs) in biological organ tissues. The optimum extraction and cleanup procedures were examined using accelerated solvent extraction (ASE), automated gel permeation chromatography (GPC) on Biobeads S-X3 and automated solid phase extraction (SPE) on silica-gel. The target compounds were divided into two fractions, non-polar compounds and more polar compounds, which in the latter fraction was subsequently methylated using diazomethane. Detection can be achieved by GC/MS in negative chemical ionization (NCI) mode. The average recoveries of the compounds spiked in swine liver, heart, kidney, and cattle adipose tissues were considered satisfactory, and it was confirmed that the method could be used in routine analysis.
Dioxin Research Group, Saitama Institute of Public Health, 639-1, Kamiokubo, Saitama 338-0824, Japan.
Koichi
K
Saito
Andreas
A
Sjödin
Courtney D
CD
Sandau
Mark D
MD
Davis
Hiroyuki
H
Nakazawa
Yasuhiko
Y
Matsuki
Donald G
DG
Patterson
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D003978:Diazomethane; D008401:Gas Chromatography-Mass Spectrometry; D006846:Hydrocarbons, Halogenated / Q000032:analysis*; D012997:Solvents; D013552:Swine; D014481:United States; D014781:Viscera / Q000737:chemistry*
D006846:Hydrocarbons, Halogenated; D012997:Solvents; D003978:Diazomethane