Preparation and characterization of polyclonal antibodies against VLDL receptor.
The polyclonal antibodies against VLDL receptor were prepared and identified. Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The collected blood serum of the immunized rabbits was analyzed and identified by using ELISA and Western Blot. The results showed that the rabbit against mouse and human VLDL receptor antibodies were obtained with high titer and could recognize the natural VLDL receptors through Western blot. The prepared polyclonal antibodies against VLDL receptor provide a new tool to study the protein of VLDL receptor.
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Shen
S
Qu
Tao
T
Chen
Fan
F
Wu
Yanhua
Y
Yin
Hao
H
Bi
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D011973:Receptors, LDL / Q000276:immunology*
D000906:Antibodies; D010455:Peptides; D011973:Receptors, LDL; C036053:VLDL receptor