Upregulation of the transcript level of GTPase activating protein KIAA0603 in T cells from patients with atopic dermatitis.
We have analyzed transcription profiles in peripheral blood CD3(+) cells from patients with allergic diseases to better understand the genes that are involved. Transcription levels of the gene KIAA0603/AS160 in CD3(+) cells from patients with atopic dermatitis (AD) were significantly higher than in normal individuals. The KIAA0603 gene encodes a 1299 amino acid protein with two phosphotyrosine interaction domains at the N-terminal region and a TBC domain at the C-terminal region. The region containing the TBC domain has a 31% homology to human rab6 GTPase activating protein (GAP). When human primary CD3(+) cells were stimulated with anti-CD3 or calcium ionophore, the KIAA0603 transcript level was upregulated. The marked upregulation of KIAA0607 was accompanied by activation induced cell death of primary CD3+ cells. KIAA0603 is likely to be a Rab GAP that participates in the regulation of activated T cells, especially helper memory T cells. Expression of KIAA0603 in T cells may be involved in pathogenesis of AD.
Matsumoto
Yoshiko
Y
Genox Research, Inc., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
Imai
Yukiho
Y
Lu Yoshida
Ning
N
Sugita
Yuji
Y
Tanaka
Toshio
T
Tsujimoto
Gozoh
G
Saito
Hirohisa
H
Oshida
Tadahilo
T
D001483:Base Sequence; D017931:DNA Primers; D003876:Dermatitis, Atopic / Q000276:immunology / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D020690:GTPase-Activating Proteins; C518767:TBC1D4 protein, human; D020691:rab GTP-Binding Proteins