Hepatic cell apoptosis was triggerred by HBx accumulation and independent on verapamil.
In order to study the roles of HBx and calcium inhibitor verapamil in apoptosis of human normal hepatic cells, L02-off, a pTet-off stably integrated human hepatic cell line was established, in which HBx expression was tightly induced by Doxycycline. The effect of different amounts of HBx and verapamil on apoptosis of human normal hepatic cells was detected. The study showed that apoptosis was triggered by accumulation of intracellular HBx, while verapamil had no effects on the apoptotic process. It was concluded that apoptosis mediated by HBx was dose-dependent but calcium-independent.
Wang
Haiping
H
Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Chen
Xiaoping
X
Bai
Xiangjun
X
D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006511:Hepatitis B Antigens / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D015534:Trans-Activators / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology*; D054334:Viral Regulatory and Accessory Proteins
D002121:Calcium Channel Blockers; D006511:Hepatitis B Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D014700:Verapamil; D002118:Calcium