[Expression and signification of pigment epithelium-derived factor in experimental choroidal neovascularization of rat].
OBJECTIVE
To investigate the expression and signification of pigment epithelium-derived factor in choroidal neovascularization (CNV) induced by krypton laser on Brown Norway rat.


METHODS
Five groups of 30 rats were photocoagulated by krypton laser in single eye to induce CNV. 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks after photocoagulation, The retina was processed for histopathology, in situ hybridization of PEDF mRNA, immunohistochemistry of PEDF and FVIIIR:Ag.


RESULTS
CNV was formed 1 week after photocoagulation, and reached its peak at 3 weeks. PEDF mRNA, PEDF expressed in the ganglion cells, inner nuclear layer, retinal pigment epithelial cells in normal BN retina. After photocoagulation, the expression of PEDF mRNA and PEDF was observed in the ganglion cells, inner nuclear layer, retinal pigment epithelial cells, and the lesions of outer nuclear layer and choroid; the expression of PEDF in the lesions was weaker than that at the edge of the lesions. From 3 days to 4 weeks, the expression density of FVIIIR:Ag gradually increased, however, the expression density of PEDF mRNA and PEDF in the lesions decreased (P < 0.05). The expression of FVIIIR:Ag was inversely correlated with that of PEDF during the development of CNV (r = -0.84, P < 0.05).


CONCLUSIONS
CNV is inversely correlated with PEDF. The down-regulation of PEDF may be one of the main reasons for the formation and development of CNV.
Shi
Xue-hui
XH
Department of Ophthalmology, General Hospital of PLA, Beijing 100853, China.
He
Shou-zhi
SZ
Zhao
Shi-hong
SH
D000818:Animals; D020256:Choroidal Neovascularization / Q000378:metabolism* / Q000473:pathology; D005136:Eye Proteins / Q000096:biosynthesis*; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D051381:Rats; D011914:Rats, Inbred BN; D012160:Retina / Q000378:metabolism / Q000473:pathology; D015843:Serpins / Q000096:biosynthesis*; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor