Experimental squalene adjuvant. II. Harmlessness and local reactogenity.
Model experiments on laboratory animals (guinea pigs) were carried out to test the possible allergic reaction (possibility of sensitisation) to the repeated administration of an experimental lipoid adjuvant prepared on the basis of squalene (experimental squalene adjuvant--ESA). No significant differences were observed between the animals sensitised-provoked with ESA and control animals. In order to evaluate the local tissue reactivity (local reactogenity), also with regard to the process dynamics to the administration of ESA, comparative patho-anatomical and patho-histological examinations of tissues were carried out in the location of adjuvant administration. The examinations indicated very low local reactogenity of the experimental lipoid adjuvant prepared in our laboratory. The test of pyrogenicity also confirmed the safety of ESA, the labelled lysate sensitivity lambda was under 0.25 IU/cm3.
Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Kosice, Slovak Republic.
Zdenek
Z
Benísek
Judit
J
Süli
Daniel
D
Eliás
L'udovít
L
Lenhardt
Anna
A
Ondrejková
Róbert
R
Ondrejka
Stefan
S
Svrcek
Viera
V
Bajová
D000276:Adjuvants, Immunologic / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D004339:Drug Compounding; D004487:Edema / Q000473:pathology; D004655:Emulsions; D005334:Fever / Q000503:physiopathology; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology; D009821:Oils; D013185:Squalene / Q000009:adverse effects* / Q000494:pharmacology; D014867:Water
D000276:Adjuvants, Immunologic; D004655:Emulsions; D009821:Oils; D014867:Water; D013185:Squalene