[Effects of rare earth compounds on human peripheral mononuclear cell telomerase and apoptosis].
OBJECTIVE
To study the effects of rare earth exposure on human telomerase and apoptosis of human peripheral mononuclear cells (PBMNs).


METHODS
Rare earth mine lot in Xunwu county, the biggest ion absorptive rare earth mine lot of China, was selected as the study site. Another village of Xunwu county, with comparable geological structure and social environment was selected as the control site. Thirty healthy adults were randomly selected from the study site as exposure group and another 30 healthy adults randomly selected from the control site as control group. The blood content of 15 rare earth elements, including La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu and Y, were determined by inductive coupled plasma-source mass spectrometry (ICP-MS). The total contents of rare earth elements in the blood were calculated. The TRAP and FCM assays were carried out to analyse the telomerase and apoptosis of human PBMNCs respectively.


RESULTS
In the exposure group, the concentration of La, Ce, Dy and Y were significantly higher (P<0.001), and Pr, Nd, Sm, Gd and Yb were higher than those in the control group (P<0.05). The total content of rare earth in the blood of exposure group showed significant difference compared with control group (P<0.001). Telomerase activity in PBMNs of the exposure group was higher than that in the control group (P<0.05); there were 11 adults in the exposure group (30 adults) and 5 adults in control group (30 adults) showed positive telomerase activity. The average age of the exposure group was (38.69 +/- 8.02) years-old, while the control group was (40.45 +/- 9.02) years-old (P >0.05). It was found that there was a significant relationship between telomerase activity and the total content of rare earth elements (P <0.01). 3. The proportion of apoptosis was not different between the two groups (P >0.05), but the cells in the S-phase and G2-M phase were increased (P <0.01) in the exposed group.


CONCLUSIONS
The telomerase activity of PBMNs in the rare earth elements exposed group was higher than that of the control group, and there is no effect on apoptotic rate of PBMNs, but may promote the diploid DNA replication, and increase the percentage of G2/M and S phase cells.
The Second Affiliated Hospital of Jiangxi Medical College, Nanchang 330006, China.
Li
L
Yu
Yu-Cheng
YC
Dai
Zhao-Kang
ZK
Yuan
Jie
J
Li
D017209:Apoptosis / Q000187:drug effects*; D004781:Environmental Exposure / Q000009:adverse effects; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008674:Metals, Rare Earth / Q000009:adverse effects* / Q000032:analysis; D019098:Telomerase / Q000378:metabolism*
D008674:Metals, Rare Earth; D019098:Telomerase