Use of capillary (zone) electrophoresis for determining felinine and it's application to investigate the stability of felinine.
A rapid capillary electrophoresis method was established to quantify felinine (2-amino-7-hydroxy-5,5-dimethyl-4-thiaheptanoic acid) in cat urine and used to investigate felinine stability. Synthetic felinine was stable in the presence of oxygen while 11% of the natural felinine in urine disappeared after 4 days exposure to air. Both synthetic felinine and the natural felinine (in urine) were stable for up to 3 months when stored at -5 degrees C and 20 degrees C. Thirty percent of the synthetic felinine was lost after 5 hours at 100 degrees C while 95% of the natural felinine disappeared after only 2 hours at the same temperature. The recovery of felinine under certain conditions was greater than 100%. It is possible that acetyl-felinine may be present in the urine and that it is deacetylated during incubation. Overall synthetic felinine was found to be stable but the felinine in cat urine much less so. Other compounds present in the urine may contribute to the decomposition of felinine.
Rutherfurd
S M
SM
Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. S.M.Rutherfurd@massey.ac.nz
Zhang
F
F
Harding
D R K
DR
Woolhouse
A D
AD
Hendriks
W H
WH
D000818:Animals; D000440:Butanols / Q000737:chemistry; D002415:Cats; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D010100:Oxygen / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014556:Urine / Q000737:chemistry
D000440:Butanols; C098250:felinine; D003545:Cysteine; D010100:Oxygen