Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages.
The phagocytosis of pathogens is a critical event in host defense, not only for clearance of the invading microorganism, but also for the subsequent immune response. We have examined Dectin-1, a proinflammatory nonopsonic receptor for beta-glucans, and show that it mediates the internalization of beta-glucan-bearing ligands, including yeast particles. Although requiring tyrosine phosphorylation and the cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM)-like motif, uptake mediated by Dectin-1 was different from any previously reported phagocytic receptor and was not dependent on Syk-kinase in macrophages. Furthermore, intracellular trafficking of this receptor was influenced by the nature of the beta-glucan ligand, which has significance for the biologic activity of these immunomodulatory carbohydrates.
Herre
Jurgen
J
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Marshall
Andrew S J
AS
Caron
Emmanuelle
E
Edwards
Alexander D
AD
Williams
David L
DL
Schweighoffer
Edina
E
Tybulewicz
Victor
V
Reis e Sousa
Caetano
C
Gordon
Siamon
S
Brown
Gordon D
GD
D016475:3T3 Cells; D000818:Animals; D005936:Glucans / Q000378:metabolism; D037181:Lectins, C-Type; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008565:Membrane Proteins / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000494:pharmacology / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D012756:Sheep; D000099181:Dectin-1
D005936:Glucans; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011505:Protein-Tyrosine Kinases; D000099181:Dectin-1