Dopamine as a robust anchor to immobilize functional molecules on the iron oxide shell of magnetic nanoparticles.
We report on the use of dopamine (DA) as a robust molecular anchor to link functional molecules to the iron oxide shell of magnetic nanoparticles. Using nitrilotriacetic acid (NTA) as the functional molecule, we created a system with an M/Fe2O3-DA-NTA (M = Co or SmCo5.2) nanostructure, which possesses high stability and specificity for separating histidine-tagged proteins. The well-established biocompatibility of iron oxide and the robust covalent bonds between DA and Fe2O3 render this strategy attractive for constructing biofunctional magnetic nanoparticles containing iron oxide.
Xu
Chenjie
C
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Xu
Keming
K
Gu
Hongwei
H
Zheng
Rongkun
R
Liu
Hui
H
Zhang
Xixiang
X
Guo
Zhihong
Z
Xu
Bing
B
D004298:Dopamine / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D006639:Histidine / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D008164:Luminescent Proteins / Q000302:isolation & purification; D008280:Magnetics; D036103:Nanotechnology; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D010316:Particle Size; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D005290:Ferric Compounds; D007093:Imidazoles; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D006639:Histidine; C029899:imidazole; D009571:Nitrilotriacetic Acid; D004298:Dopamine