[Protection of NT3 gene transfection on the guinea pig cochlea treated with gentamicin].
OBJECTIVE
To study the protection of NT3 gene transfection on the guinea pig cochlea treated with gentamicin.


METHODS
The liposome plasmid complex (pIRES2-EGFP-NT3) was injected into the guinea pig cochlea, then the guinea pigs were treated with gentamicin (120 mg/kg, 14 d). The DPOAE and ABR were measured at 1 d and 2 w after stop the gentamicin and the expression of NT-3 was observed respectively, and the hair cells were observed.


RESULTS
Compared with the group of NT3, the DPOAE of the guinea pigs transfected with blank vector and PBS were evidently dropped (P<0.05). The ABR thresholds were similar with the results of DPOAE. The hair cells with the FITC-phalloidin immunohistochemical dyeing showed that the absence of hair cells in the groups of NT3 were little severity than the compared groups.


CONCLUSIONS
This study showed that cochlear NT3 gene transfection could provide safe and effective production to the guinea pigs treated with gentamicin.
Department of Otolaryngology, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, China.
Zhihong
Z
Deng
Jinling
J
Wang
Jianhua
J
Qiu
Shunli
S
Liu
Chengji
C
Wang
Angang
A
Yang
D000818:Animals; D003051:Cochlea / Q000187:drug effects*; D003638:Deafness / Q000139:chemically induced / Q000628:therapy*; D015316:Genetic Therapy; D005839:Gentamicins / Q000009:adverse effects*; D006168:Guinea Pigs; D020933:Neurotrophin 3 / Q000235:genetics*; D014162:Transfection
D005839:Gentamicins; D020933:Neurotrophin 3