Serum levels of different forms of soluble CD38 antigen in burned patients.
The level of the total and dimeric (oligomeric) forms of soluble CD38 antigen (sCD38) has been determined by an ELISA sandwich method in serum from burned patients (n=18) and healthy volunteers (n = 25). The serum level of total sCD38 was insignificantly increased in patients at the stage of burn shock (135 +/- 10.8 U/ml, mean +/- S.E.M.) and significantly decreased between 4 and 14 postburn days in comparison with volunteers (69.5 +/- 10.8 U/ml versus 121 +/- 7.8 U/ml, P < 0.05). The serum level of soluble dimeric CD38 in burned patients was statistically lower than normal during all periods of observation (45.3 +/- 8.8 and 130 +/- 6.2 U/ml, respectively, P < 0.01). The relative number of CD38(+) lymphocytes was increased during the period of shock in comparison with healthy volunteers (21 +/- 1.6% versus 13 +/- 1.1%, P < 0.05). There were no correlations between number CD38(+) lymphocytes and total sCD38 or dimeric sCD38 serum levels. These data suggest that the mCD38 expression and serum level of total sCD38 are a markers the early postburn lymphocytes activation. The decrease of dimeric sCD38 level can reflect its dissociation to monomeric form in burned patients.
Lebedev
M Ju
MJ
Laboratory of Immunology, Institute of Traumatology and Orthopaedics, 603155 Verhne Volzhskaya nab. 18 Nizhny Novgorod, Russian Federation. miklgito@mail.ru
Egorova
N I
NI
Sholkina
M N
MN
Vilkov
S A
SA
Baryshnikov
A Ju
AJ
Novikov
V V
VV
D036541:ADP-ribosyl Cyclase / Q000097:blood*; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D002056:Burns / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008562:Membrane Glycoproteins; D012995:Solubility; D013997:Time Factors
D015703:Antigens, CD; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1