PrPc and reggies/flotillins are contained in and released via lipid-rich vesicles in Jurkat T cells.
A new model of caveolin association with lipid body cores has recently been proposed which may be relevant to a number of cellular processes, e.g. lipid body generation. Here we show that PrPc and reggie-1 and reggie-2 also occur in the cores of Nile Red/Bodipy-stained (neutral lipid-containing) vesicular structures and, in immunoblots, in the lipid-enriched fraction after density gradient centrifugation. These lipid-rich vesicles increase in number following cell feeding with oleic acid, differ from early endosome antigen 1- and Lamp-2-positive endosomes/lysosomes, exhibit an opaque content and lack surrounding actin staining. Our results suggest that the content of these vesicles, together with reggie-1 and -2 and PrPc, is expelled.
Department of Biology, University of Konstanz, 78457, Konstanz, Germany. alexander.reuter@uni-konstanz.de
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D000199:Actins / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D010078:Oxazines; D018621:PrPC Proteins / Q000378:metabolism*; D013194:Staining and Labeling
D000199:Actins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D010078:Oxazines; D018621:PrPC Proteins; C106354:flotillins; C044808:nile red