Phosphorylation regulates Id3 function in vascular smooth muscle cells.
Understanding the mechanisms that regulate cell cycle progression in vascular smooth muscle cells (VSMCs) is key to understanding and modulating vascular lesion formation. Results of the present study provide the first evidence that phosphorylation of the helix-loop-helix factor Id3 in VSMCs occurs in vitro and in vivo and provides a regulatory switch controlling Id3-induced regulation of p21Cip1 and VSMC growth.
Cardiovascular Division, Department of Internal Medicine, and the Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA.
Scott T
ST
Forrest
Angela M
AM
Taylor
Ian J
IJ
Sarembock
Demetra
D
Perlegas
Coleen A
CA
McNamara
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001011:Aorta; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D000077214:Becaplermin; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21; D005786:Gene Expression Regulation; D006801:Humans; D006937:Hypercholesterolemia / Q000235:genetics / Q000378:metabolism; D051796:Inhibitor of Differentiation Proteins; D051379:Mice; D018345:Mice, Knockout; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D001057:Apolipoproteins E; C493956:CDKN1A protein, human; C493957:Cdkn1a protein, mouse; C493958:Cdkn1a protein, rat; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; C499848:Id3 protein, rat; D051796:Inhibitor of Differentiation Proteins; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D020574:Proto-Oncogene Proteins c-sis; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D000804:Angiotensin II; C067679:Idb3 protein, mouse; D000077214:Becaplermin