Proteomics to display tissue repair opposing injury response to LPS-induced liver injury.
OBJECTIVE
To examine the protein expression alterations in liver injury/repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver injury.


METHODS
Male BALB/c mice were treated with intra-peritoneal (i.p.) LPS (4 mg/kg) and sacrificed at 0, 6, 24 and 30 h to obtain livers. The livers were stained with hematoxylin and eosin for histopathologic analyses. Total liver protein was separated by two-dimensional gel electrophoresis (2-DE). The peptide mass of liver injury or repair related proteins were drawn up and the protein database was searched to identify the proteins.


RESULTS
Observations were as follows: (1) TRAIL-R2 was down regulated in livers of LPS-treated mice. TNFAIP1 was significantly up regulated at 6 h, then down- regulated at 24, 30 h with silent expression during senescent stage. (2) The amount of metaxin 2 and mitochondria import inner membrane translocase subunit TIM8a (TIMM8A) was increased upon treatment with LPS. (3) P34 cdc2 kinase was significantly up-regulated 30 h after LPS administration with silent expression during senescent, 6, 24 h treated stage. (4) The amount of proteasome activator 28 alpha subunit (PA28), magnesium dependent protein phosphatase (MDPP) and lysophospholipase 2 was decreased 6 h after LPS treatment but recovered or up-regulated 24 and 30 h after LPS treatment.


CONCLUSIONS
LPS-treated mouse liver displaying a time-dependent liver injury can result in expression change of some liver injury or repair related proteins.
Liu
Xiao-Wei
XW
Department of Gastroenterology, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
Lu
Fang-Gen
FG
Zhang
Guang-Sen
GS
Wu
Xiao-Ping
XP
You
Yu
Y
Ouyang
Chun-Hui
CH
Yang
Dong-Ye
DY
D000818:Animals; D015415:Biomarkers; D015180:Electrophoresis, Gel, Two-Dimensional; D008070:Lipopolysaccharides / Q000633:toxicity*; D008107:Liver Diseases / Q000473:pathology / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics; D015398:Signal Transduction / Q000502:physiology
D015415:Biomarkers; D008070:Lipopolysaccharides