Modelling the stratum corneum lipid organisation with synthetic lipid mixtures: the importance of synthetic ceramide composition.
Cholesterol (CHOL), free fatty acids (FFA) and nine classes of ceramides (CER1-CER9) form the main constituents of the intercellular lipid lamellae in stratum corneum (SC), which regulate the skin barrier function. Both the presence of a unique 13-nm lamellar phase, of which the formation depends on the presence of CER1, and its dense lateral packing are characteristic for the SC lipid organisation. The present study focuses on the lipid organisation in mixtures prepared with CHOL, FFA and a limited number of synthetic CER, namely CER1, CER3 and bovine brain CER type IV (SigmaCERIV). The main objective is to determine the optimal molar ratio of CER3 to SigmaCERIV for the formation of the 13-nm lamellar phase. CER3 contains a uniform acyl chain length, whereas SigmaCERIV contains fatty acids with varying chain lengths. Using small angle X-ray diffraction (SAXD), it is demonstrated that the CER3 to SigmaCERIV ratio affects the formation of the 13-nm lamellar phase and that the optimal ratio depends on the presence of FFA. Furthermore, the formation of the 13-nm lamellar phase is not very sensitive to variations in the total CER level, which is similar to the in vivo situation.
Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Department of Drug Delivery Technology, University of Leiden, PO Box 9502, 2300 RA, The Netherlands.
Miranda W
MW
de Jager
Gert S
GS
Gooris
Igor P
IP
Dolbnya
Maria
M
Ponec
Joke A
JA
Bouwstra
D002518:Ceramides / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis; D004817:Epidermis / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D014961:X-Ray Diffraction
D002518:Ceramides; D005227:Fatty Acids; D008055:Lipids; D002784:Cholesterol