The AcrAB-TolC pump is involved in macrolide resistance but not in telithromycin efflux in Enterobacter aerogenes and Escherichia coli.
The role of the AcrAB-TolC pump in macrolide and ketolide susceptibility in Escherichia coli and Enterobacter aerogenes was studied. Efflux pump inhibitor restored erythromycin, clarithromycin, and telithromycin susceptibilities to multidrug-resistant isolates. No modification of telithromycin accumulation was detected in E. aerogenes acrAB or tolC derivatives compared to that in the parental strain. Two independent efflux pumps, inhibited by phenylalanine arginine beta-naphthylamide, expel macrolides and telithromycin in E. aerogenes.
Enveloppe Bactérienne, Perméabilité et Antibiotique, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
Renaud
R
Chollet
Jacqueline
J
Chevalier
André
A
Bryskier
Jean-Marie
JM
Pagès
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000235:genetics; D021902:Enterobacter aerogenes / Q000187:drug effects* / Q000378:metabolism* / Q000472:pathogenicity; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D048628:Ketolides; D018942:Macrolides / Q000378:metabolism* / Q000494:pharmacology*; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D048628:Ketolides; D018942:Macrolides; D026901:Membrane Transport Proteins; C073126:tolC protein, E coli; D004917:Erythromycin; C029772:arginine beta-naphthylamide; D001120:Arginine; C106791:telithromycin