Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique.
A DNA array has been fabricated on glass substrates, which enables high-throughput analysis of single-base mismatches. In this work, microfabrication-compatible plasma-polymerization (PP) method was used for immobilizing probe DNAs to study the hybridization behavior by changing surface properties. The immobilization matrix consisting of 35 A of PP layer, applied additionally on the streptavidin absorbed hexamethyldisiloxane (HMDS)-PP layer, was constructed on the substrates to anchor biotinylated DNA probes onto the surface. The hydrophobic immobilization matrix was considered to enhance hybridization accuracy and efficiency, compared with its hydrophilic acetonitrile-PP layers. The oligonucleotide arrays fabricated on HMDS-PP surface were shown to be effective in detection of single nucleotide polymorphisms (SNPs) of ApoE gene.
Miyachi
Hirotaka
H
Laboratory of Advanced Bioelectronics, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 4, 1-1-1 Higashi, Tsukuba-city, Ibaraki 305-8562, Japan.
Ikebukuro
Kazunori
K
Yano
Kazuyoshi
K
Aburatani
Hiroyuki
H
Karube
Isao
I
D001057:Apolipoproteins E / Q000032:analysis / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D008420:Manufactured Materials; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D001057:Apolipoproteins E; D020099:Coated Materials, Biocompatible; D015342:DNA Probes; D011108:Polymers