Development of a rat model of graded contusive spinal cord injury using a pneumatic impact device.
An animal model of spinal cord trauma is essential for understanding the injury mechanisms, cord regeneration, and to aid the development of new therapeutic modalities. This study focused on the development of a graded experimental contusion model for spinal cord injury (SCI) using a pneumatic impact device made in Korea. A contusive injury was made to the dorsal aspect of the cord. Three trauma groups were defined according to the impact velocity (IV). A control group (n=6), received laminectomy only. Group 1 (n=10), 2 (n=10), and 3 (n=10) had IVs of 1.5 m/sec, 2.0 m/sec, and 3.5 m/sec respectively. Functional assessments were made up to the 14th day after injury. The cord was removed at the 14th postinjury day and prepared for histopathologic examination. Significant behavioral and histopathological abnormalities were found in control and each trauma group. All trauma groups showed severe functional impairment immediately after injury but following different rates of functional recovery (Fig. 5). As the impact velocity and impulse increased, the depth of contusive lesion revealed to be profound the results show that the rat model reproduces spinal cord lesions consistently, has a distinctive value in assessing the effects of impact energy.
Department of Neurosurgery, College of Medicine, Chung-Ang University, Seoul, Korea.
Sang Jun
SJ
Yeo
Sung Nam
SN
Hwang
Seung Won
SW
Park
Young Baeg
YB
Kim
Byung Kook
BK
Min
Jeong Taik
JT
Kwon
Jong Sik
JS
Suk
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D006801:Humans; D007796:Laminectomy; D008297:Male; D023421:Models, Animal; D009043:Motor Activity / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy