Measurement of cortical and hippocampal epileptiform activity in freely moving rats by means of implantable radiotelemetry.
Implanted radiotelemetry has been used for the measurement of cortical electroencephalogram (EEG), locomotor activity, body temperature and cardiovascular parameters. This technique allows high quality data acquisition from freely moving animals with no complications of externalised apparatus. This paper focuses on the methodology for short and long-term monitoring of epileptiform activity by simultaneous cortical EEG, hippocampal (HC) EEG and electromyogram (EMG) in rats. The circadian rhythm of temperature (CRT) was monitored after surgery to estimate the need for post surgical recovery of animals. Different placements of EMG electrodes were assessed in order to minimise artefacts and increase sensitivity. The occurrence of epileptiform ictal and interictal activity following an acute injection of either 40 mg/kg pentylenetetrazole (PTZ) or 13.8 mg/kg kainic acid (KA) was investigated. The occurrence of spontaneous seizures was also monitored 5-8 weeks after administration of KA. The present study demonstrated a sensitive method for monitoring cortical EEG, hippocampal EEG and EMG short and long-term by implantable radiotelemetry in freely moving rats.
Department of Neuropharmacology, H. Lundbeck A/S, Ottiliavej 9, 2500 Valby, Denmark. jfb@lundbeck.com
Jesper F
JF
Bastlund
Poul
P
Jennum
Paul
P
Mohapel
Vanessa
V
Vogel
William P
WP
Watson
D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000503:physiopathology*; D004567:Electrodes, Implanted; D004569:Electroencephalography / Q000295:instrumentation / Q000379:methods*; D004576:Electromyography / Q000379:methods; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology*; D006624:Hippocampus / Q000503:physiopathology*; D007608:Kainic Acid; D008297:Male; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D013686:Telemetry / Q000295:instrumentation; D013997:Time Factors; D014851:Wakefulness / Q000502:physiology*
D007608:Kainic Acid; D010433:Pentylenetetrazole