Defining the genome content of live plague vaccines by use of whole-genome DNA microarray.
Yersinia pestis whole-genome DNA microarrays were developed to perform genomic comparison of a collection of live plague vaccines. By using the genomic DNA to probe the DNA microarrays, we detected dozens of deletions and amplifications of the genomic regions in the 19 vaccine strains analyzed. The revealed genomic differences within the vaccine strains of different origins provide us an unprecedented opportunity to understand the molecular background of the variability of the immunogenic and protective potency of plague live vaccine. The whole-genome DNA microarray also provides an ideal tool to perform the pre-evaluation of a vaccine strain for its high throughput to determine the genomic features essential or unallowable for the live vaccines.
Zhou
Dongsheng
D
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing 100071, PR China.
Han
Yanping
Y
Dai
Erhei
E
Song
Yajun
Y
Pei
Decui
D
Zhai
Junhui
J
Du
Zongmin
Z
Wang
Jin
J
Guo
Zhaobiao
Z
Yang
Ruifu
R
D002876:Chromosomes, Bacterial / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010930:Plague / Q000382:microbiology; D010931:Plague Vaccine / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014613:Vaccines, Attenuated / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology*
D004269:DNA, Bacterial; D010931:Plague Vaccine; D014613:Vaccines, Attenuated