Analysis of radiolytic products of lipid in irradiated dried squids (Todarodes pacificus).
The lipid portion of dried squids (Todarodes pacificus) was extracted, and its hydrocarbons and 2-alkylcyclobutanones were separated using a florisil column. Both compounds were identified by gas chromatography and mass spectrometry and used to investigate the production of radiation-induced hydrocarbons and 2-alkylcyclobutanones. Concentrations of the hydrocarbons and 2-alkylcyclobutanones increased linearly with the radiation dosage. The major hydrocarbons in the irradiated dried squids were pentadecane and 1-tetradecene, which originated from palmitic acid. The amount of pentadecane was the highest among the radiation-induced hydrocarbons in the dried squids. The major 2-alkylcyclobutanone in the irradiated dried squids was 2-dodecylcyclobutanone, which was formed from the large amount of palmitic acid. 2-Tetradecylcyclobutanone, which may be produced from stearic acid in sample lipids, was also detected. Radiation-induced hydrocarbons and 2-alkylcyclobutanones were detected at > or = 0.5 kGy. These compounds were not detected in dried squids that were not irradiated. Radiation-induced hydrocarbons can be used as a detection marker for irradiated dried squids; however, the amount of 2-alkylcyclobutanones produced was not enough to be used as a marker. Radiolytic products of lipids, such as hydrocarbons or 2-alkylcyclobutanones. can be used to monitor food safety for consumers, ensuring proper irradiation labeling in foods and quarantine treatment in international trade.
Kim
Jun-Hyoung
JH
Department of Food and Nutrition, Chosun University, Gwangju 501-759, Korea.
Seo
Hye-Young
HY
Kim
Kyong-Su
KS
D000818:Animals; D002074:Butanones / Q000032:analysis; D003257:Consumer Product Safety; D049832:Decapodiformes / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005227:Fatty Acids / Q000528:radiation effects*; D005514:Food Irradiation; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis
D002074:Butanones; D005227:Fatty Acids; D006838:Hydrocarbons