Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule.
Intercellular adhesion molecule 1 (ICAM-1) is the cellular receptor for the major group of human rhinovirus serotypes, including human rhinovirus 14 (HRV14) and HRV16. A naturally occurring variant of ICAM-1, ICAM-1Kilifi, has altered binding characteristics with respect to different HRV serotypes. HRV14 binds to ICAM-1 only transiently at physiological temperatures but forms a stable complex with ICAM-1Kilifi. Conversely, HRV16 forms a stable complex with ICAM-1 but does not bind to ICAM-1Kilifi. The three-dimensional structures of HRV14 and HRV16, complexed with ICAM-1, and the structure of HRV14, complexed with ICAM-1Kilifi, have been determined by cryoelectron microscopy (cryoEM) image reconstruction to a resolution of approximately 10 angstroms. Structures determined by X-ray crystallography of both viruses and of ICAM-1 were fitted into the cryoEM density maps. The interfaces between the viruses and receptors contain extensive ionic networks. However, the interactions between the viruses and ICAM-1Kilifi contain one less salt bridge than between the viruses and ICAM-1. As HRV16 has fewer overall interactions with ICAM-1 than HRV14, the absence of this charge interaction has a greater impact on the binding of ICAM-1Kilifi to HRV16 than to HRV14.
Department of Biological Sciences, Purdue University, 915 W. State St., West Layfayette, IN 47909-2054, USA.
Chuan
C
Xiao
Tobias J
TJ
Tuthill
Carol M
CM
Bator Kelly
Lisa J
LJ
Challinor
Paul R
PR
Chipman
Richard A
RA
Killington
David J
DJ
Rowlands
Alister
A
Craig
Michael G
MG
Rossmann
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D012229:Rhinovirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics
D046911:Macromolecular Substances; D011991:Receptors, Virus; D014764:Viral Proteins; D018799:Intercellular Adhesion Molecule-1