Immobilization of lipase from Candida rugosa on layered double hydroxides for esterification reaction.
Synthesis of layered double hydroxides (LDHs) of Zn/Al-NO3- hydrotalcite (HIZAN) and Zn/Al-diocytyl sodium sulfosuccinate (DSS) nanocomposite (NAZAD) with a molar ratio of Zn/Al of 4:1 were carried out by coprecipitation through continuous agitation. Their structures were determined using X-ray diffractometer spectra, which showed that basal spacing for LDH synthesized by both methods was about 8.89 A. An expansion of layered structure of about 27.9 A was observed to accommodate the surfactant anion between the interlayer. This phenomenon showed that the intercalation process took place between the LDH interlayer. Lipase from Candida rugosa was immobilized onto these materials by physical adsorption method. It was found that the protein loading onto NAZAD is higher than HIZAN. The activity of immobilized lipase was investigated through esterification of oleic acid and 1-butanol in hexane. The effects of pore size, surface area, reaction temperature, thermostability of the immobilized lipases, storage stability in organic solvent, and leaching studies were investigated. Stability was found to be the highest in the nanocomposite NAZAD.
Rahman
Mohd Basyaruddin A
MB
Centre for Research in Enzyme & Microbial Technology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor. basya@fsas.upm.edu.my
Basri
Mahiran
M
Hussein
Mohd Zobir
MZ
Rahman
Raja Nor Zaliha A
RN
Zainol
Dara Hatira
DH
Salleh
Abu Bakar
AB
D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D006586:Hexanes; D006878:Hydroxides / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning
D004800:Enzymes, Immobilized; D006586:Hexanes; D006878:Hydroxides; D008049:Lipase