Protective efficacy of IgA monoclonal antibodies to O and H antigens in a mouse model of intranasal challenge with Salmonella enterica serotype Enteritidis.
Protective properties of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis (S. enteritidis) were evaluated in a model of generalized infection after intranasal (i.n.) inoculation of BALB/c mice. Passive i.n. instillation of antibodies 1 h before i.n. challenge did not prevent infection, and mice developed rapid inflammatory response in the lower respiratory tract. The passive systemic immunization was partially protective and a single intravenous (i.v.) injection of both O and H antigen specific IgA antibodies prolonged survival period of the infected animals. Permanent secretion of O:9 specific IgA MAb 177E6 into the respiratory tract in a "backpack" tumor model protected 50% of animals infected i.n. with a high dose of virulent S. enteritidis strain. Thus, secretory IgA (S-IgA) directed against O:9 antigen alone can prevent bacterial invasion in the respiratory epithelium.
Department of Microbiology, Medical University of Sofia, Zdrave 2 street, 1431 Sofia, Bulgaria. ianko@medfac.acad.bg
Ianko D
ID
Iankov
Dragomir P
DP
Petrov
Ivan V
IV
Mladenov
Iana H
IH
Haralambieva
Ognian K
OK
Kalev
Maria S
MS
Balabanova
Ivan G
IG
Mitov
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000276:immunology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007116:Immunization, Passive / Q000379:methods; D007070:Immunoglobulin A / Q000276:immunology* / Q000494:pharmacology; D007700:Kinetics; D008171:Lung Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C022863:H antigen; D007070:Immunoglobulin A; D019081:O Antigens