Increase of caspase-3 activity by lignans from Machilus thunbergii in HL-60 cells.
Nine lignans and two butanolides were isolated from the stem bark of Machilus thunbergii and their structures were identified as machilin A (1), licarin B (2), zuonin B (3), macelignan (4), secoisolancifolide (5), isolancifolide (6), oleiferin C (7), meso-dihydroguaiaretic acid (8), licarin A (9), machilin F (10), and nectandrin B (11) by spectroscopic means. These compounds were assessed for their abilities to activate a caspase-3 activity in human promyeloid leukemic HL-60 cells. The intracellular caspase-3 activity of macelignan (4), oleiferin C (7), meso-dihydroguaiaretic acid (8), and licarin A (9) increased approximately 3.04, 6.16, 2.10, and 3.10-fold at 100 microM over that of untreated control. In addition, compounds 4, 7, 8, and 9 induced internucleosomal DNA fragmentation in HL-60 cells.
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Bo-Young
BY
Park
Byung-Sun
BS
Min
Ok-Kyoung
OK
Kwon
Sei-Ryang
SR
Oh
Kyung-Seop
KS
Ahn
Tae-Jin
TJ
Kim
Doo-Young
DY
Kim
KiWhan
K
Bae
Hyeong-Kyu
HK
Lee
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D027421:Persea / Q000737:chemistry*
D017705:Lignans; C575181:isolancifolide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D015107:4-Butyrolactone