Comparison of n-eicosane and phenanthrene removal by pure and mixed cultures of two marine bacteria.
The biotransformation activities of two hydrocarbonoclastic marine bacteria, Corynebacterium sp. and Sphingomonas sp. 2MPII, on n-eicosane and phenanthrene were investigated. During a 56-day experiment, in pure and mixed cultures, Corynebacterium sp. and Sphingomonas sp. 2MPII removed about 70% of the initial n-eicosane and phenanthrene concentrations (1 and 0.4 g L(-1), respectively). In pure cultures, culturable cell abundances increased over time, from 0.8 to 8.6 x 10(-11) CFU L(-1) (Corynebacterium sp.) and from 2.1 to 16 x 10(-11) CFU L(-1) (Sphingomonas sp. 2MPII ) but remained barely constant in mixed cultures. We defined a biotransformation index based on the number of culturable cells rather than the culture protein content, with the biotransformation cell yield (BCY) expressed in grams hydrocarbon CFU(-1) per day to better characterize hydrocarbon removal in pure and mixed cultures. The BCY was markedly higher in mixed than in pure cultures, increasing by a factor of 2-10.7 and 2.3-4.7 for n-eicosane and phenanthrene removal, respectively.
Laboratoire de Chimie Analytique de l'Environnement, UMR 6171, IFR PMSE 112, Europôle de l'Arbois, University of d'Aix Maseille, BP 80, 13545 Aix-en-Provence Cedex 4, France.
A D
AD
Syakti
M
M
Acquaviva
M
M
Gilewicz
P
P
Doumenq
J C
JC
Bertrand
D000473:Alkanes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003352:Corynebacterium / Q000254:growth & development / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D010616:Phenanthrenes / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D020578:Sphingomonas / Q000254:growth & development / Q000378:metabolism*
D000473:Alkanes; D010616:Phenanthrenes; C050821:eicosane; C031181:phenanthrene