Steady-state detection of cholesterol contained in the plasma membrane of a single cell using lipid bilayer-modified microelectrodes incorporating cholesterol oxidase.
Platinum microelectrodes modified with a lipid bilayer membrane incorporating cholesterol oxidase are used for detection of cholesterol contained in the plasma membrane of a single cell. Amperometric responses are consistent with enzymatic catalysis being rate limiting and cholesterol diffusing laterally in the plasma membrane to the electrode contact site. Importantly, electrode response appears to correlate with the cholesterol content of the cell plasma membrane. The electrodes should be useful for characterizing cellular cholesterol tracking pathways involved in pathogenesis of disease.
Department of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-7078, USA.
Anando
A
Devadoss
James D
JD
Burgess
D000818:Animals; D015374:Biosensing Techniques; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008839:Microelectrodes; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D014981:Xenopus
D008051:Lipid Bilayers; D002784:Cholesterol; D002789:Cholesterol Oxidase