Laminar optical tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media.
Laminar optical tomography (LOT) is a new technique that combines the advantages of diffuse optical tomography image reconstruction and a microscopy-based setup to allow noncontact imaging with 100-200-microm resolution effective over depths of 0-2.5 mm. LOT is being developed primarily for multispectral imaging of rat cortex, for which resolving functional dynamics in various layers of the brain's cortex (to depths of 1500 microm) is of increasing interest to neurophysiologists. System design and image reconstruction techniques are described, along with simulation and phantom results that demonstrate the characteristics and limitations of system accuracy and resolution.
Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA. ehillman@nmr.mgh.harvard.edu
Elizabeth M C
EM
Hillman
David A
DA
Boas
Anders M
AM
Dale
Andrew K
AK
Dunn
D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply* / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008833:Microcirculation / Q000166:cytology*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D019047:Phantoms, Imaging; D051381:Rats; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods*