Proteoglycans and catabolic products of proteoglycans present in ligament.
The aim of the present study was to characterize the proteoglycans and catabolic products of proteoglycans present in the tensile region of ligament and explant cultures of this tissue, and to compare these with those observed in the tensile region of tendon. Approx. 90% of the total proteoglycans in fresh ligament was decorin, as estimated by N-terminal amino acid sequence analysis. Other species that were detected were biglycan and the large proteoglycans versican (splice variants V(0) and/or V1 and/or V2) and aggrecan. Approx. 23% of decorin detected in the matrix was degraded. Intact decorin and decorin fragments similar to those observed in the matrix that retained the N-terminus were also observed in the medium of ligament cultures. Intact biglycan core protein was detected in the matrix and medium of ligament cultures, and two fragments originating from the N-terminal region of biglycan were observed in the matrix of cultured ligament. Versican and versican fragments that retained the N-terminus of versican core protein were detected in fresh matrix and medium of tendon cultures. Approx. 42% of versican present in the fresh ligament was degraded. Aggrecan catabolites appearing in the culture medium were derived from aggrecanase cleavage of the core protein. An intact link protein and a degradation product from the N-terminal region of type XII collagen were also detected in the medium of the ligament explant.
School of Human Biosciences, La Trobe University, Melbourne, Victoria 3086, Australia. m.ilic@latrobe.edu.au
Mirna Z
MZ
Ilic
Phillip
P
Carter
Alicia
A
Tyndall
Jayesh
J
Dudhia
Christopher J
CJ
Handley
D053674:Aggrecans; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D058578:Biglycan; D002417:Cattle; D017885:Collateral Ligaments / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type; D008297:Male; D008662:Metacarpophalangeal Joint; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods; D046509:Tissue Culture Techniques
D053674:Aggrecans; D000911:Antibodies, Monoclonal; C546884:BGN protein, human; D058578:Biglycan; D003470:Culture Media; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D010446:Peptide Fragments; D011509:Proteoglycans