Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon.
OBJECTIVE
To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes.


METHODS
PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing.


RESULTS
Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR.


CONCLUSIONS
E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.
Boyd
David A
DA
Nosocomial Infections, National Microbiology Laboratory, Health Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada.
Kibsey
Pamela
P
Roscoe
Diane
D
Mulvey
Michael R
MR
D001426:Bacterial Proteins / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics*; D005260:Female; D006801:Humans; D007669:Kidney Calculi / Q000150:complications / Q000382:microbiology; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000150:complications / Q000382:microbiology; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D010453:Peptide Synthases; C122181:VanD protein, Enterococcus faecium