[The abnormity and clinical value of tyrosine kinase signaling pathway in lung adenocarcinoma].
OBJECTIVE
To study the abnormity and clinical value of tyrosine kinase signaling pathway in lung adenocarcinoma.


METHODS
Total RNA was extracted form 10 lung adenocarcinoma samples and matched normal tissues. cDNA was labeled fluorescence during reverse transcription and hybridized on 20 slides of microarray with 13 824 genes. After washed and scanned, the cy3:cy5 ratios were obtained by computer analysis. Each experiment was repeated two times and the mean value was chosen. Cluster analysis and discriminant analysis were used to mine the pool data. The results were combined with clinical prognostic factors for further analysis.


RESULTS
According to the abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma, sample cluster divided the 10 samples into 3 groups and gene cluster divided all genes into 5 groups. Different prognosis samples had different characteristic genes. The patients with the best prognosis overexpressed some genes related to immunity and inhibition proliferation, and those with the worst prognosis overexpressed some genes related to proliferation while the genes related to immunity and inhibition proliferation were expressed at a low level.


CONCLUSIONS
There are 3 type abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma that are related to different biological behavior and different prognosis.
Lung Cancer Research Institute; Cancer Center, Guangdong Provincial People's Hospital, Guangzhou 510080, China. gzyilong@hotmail.com
Yi-long
YL
Wu
Jia-ying
JY
Lin
Xue-ning
XN
Yang
Gui-bin
GB
Qiao
Kun
K
Wang
Gang
G
Chen
D000230:Adenocarcinoma / Q000201:enzymology*; D000368:Aged; D016000:Cluster Analysis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012313:RNA / Q000032:analysis; D015398:Signal Transduction
D012313:RNA; D011505:Protein-Tyrosine Kinases