Co-localization of Trax and Mea2 in Golgi complex of pachytene spermatocytes in the mouse.
A golgin family protein, Mea2, is expressed at enhanced level in pachytene spermatocytes and is indispensable for mouse spermatogenesis. Because Trax was shown to interact with Mea2 in yeast two-hybrid, we investigated the localization of Trax in pachytene spermatocytes with immunofluorescent staining. Trax was found to accumulate in the Golgi complex of mid-late pachytene spermatocytes and intermingled with granular Mea2 signal in the central region. In a subline of the Mea2 mutant mouse, a truncated form of Mea2 devoid of the N-terminal region, DeltaMea2, was expressed. It localized to the rim of Golgi complex and thus occupied a region separate from that of Trax.
Matsuda
Miyuki
M
Kanagawa Cancer Center Research Institute, Nakao 1-1-2, Asahi-ku, Yokohama 241-0815, Japan.
Kondo
Masaaki
M
Kashiwabara
Shin-Ichi
S
Yoshihara
Mitsuyo
M
Sutou
Shizuyo
S
Matsukuma
Shoichi
S
D000818:Animals; D001324:Autoantigens; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D013090:Spermatocytes / Q000166:cytology* / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism
D001324:Autoantigens; D004268:DNA-Binding Proteins; C512726:Golga3 protein, mouse; C512728:Mea1 protein, mouse; D008565:Membrane Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C498303:Tsn protein, mouse; C498306:Tsnax protein, mouse