Effects of granulocyte-colony stimulating factor on wound healing in a mouse model of burn trauma.
The effects of burn trauma and granulocyte-colony stimulating factor (G-CSF) treatment on wound healing in a surgical incision model were studied. Sixty adult male mice were used in this study. Under general anesthesia hot water at 97 degrees C was applied for 3 sec to the dorsum of the mice in order to achieve 20% burn wound. After burn trauma, full thickness midline skin incision 2 cm in length was performed on the abdominal wall and then were sutured primarily with 4/0 polypropylene. In Group I only skin incision was performed, group II had skin incision and burn, in group III G-CSF (0.03 BU/30 g) was applied intraperitoneally after burn and skin incision. Breaking strength and 5-hydroxyproline (5-HP) levels of the wounds were calculated 5 and 10 days after the procedure. 5-HP levels and breaking strength values showed statistical difference between groups II-III and I-II (p<0.05). 5-HP levels were lowest in incision and burn group (41.80 microg/mg). Breaking strength levels were also lowest in the same group (0.12 kg) (p<0.05). These results suggest that third degree burn causes a significant impairment on incisional wound healing and G-CSF ameliorates this impairment.
Eroglu
Erol
E
Department of General Surgery, Suleyman Demirel University, School of Medicine, Isparta, Turkey. heroleroglu@hotmail.com
Agalar
Fatih
F
Altuntas
Irfan
I
Eroglu
Fusun
F
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism / Q000627:therapeutic use*; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D051379:Mice; D011897:Random Allocation; D013718:Tensile Strength; D014945:Wound Healing
D016179:Granulocyte Colony-Stimulating Factor; D006909:Hydroxyproline