Effects of exposure to genistein during pubertal development on the reproductive system of male mice.
Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may affect adversely the development of the male reproductive system. Twenty-one days old ICR mice weaned from dams fed with a casein-based AIN-76A diet during gestation and lactation were exposed to genistein (2.5 and 5.0 mg/kg/day, p.o.) for 5 weeks. 17beta-Estradiol (7.5 microg/kg/day) and corn oil were used for the positive and negative vehicle controls, respectively. The animals were fed the casein-based AIN-76A diet throughout the experiment. There were no significant differences in body weights of mice between the genistein groups and the negative control group. No significant differences in relative reproductive organ weights were found among all experimental groups. Sperm counts in epididymis and testes were slightly decreased in the genistein-exposed groups compared with control group. Sperm motile characteristics in genistein-exposed groups were slightly higher than those of the control group. Levels of phospholipid hydroxide glutathione peroxidase mRNA in the testis, epididymis, and prostate of mice exposed to genistein or estradiol were significantly higher than those of the controls (P<0.05). Exposure to genistein caused hyperplasia of Leydig cells in the testis and a slight increase of interstitial fibroblasts in the epididymis, while estradiol treatment caused severe damage to the testis and epididymis. These results suggest that dietary uptake of genistein during the juvenile period may affect male reproductive development, resulting in a slight decrease in sperm count, but with an increase in sperm motion quality.
Lee
Beom-Jun
BJ
Department of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jung
Eun-Yong
EY
Yun
Young-Won
YW
Kang
Jong-Koo
JK
Baek
In-Jeoung
IJ
Yon
Jung-Min
JM
Lee
Yoon-Bok
YB
Sohn
Heon-Soo
HS
Lee
Jae-Yong
JY
Kim
Kang-Sung
KS
Nam
Sang-Yoon
SY
D000818:Animals; D016588:Anticarcinogenic Agents / Q000633:toxicity*; D001835:Body Weight; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology; D019833:Genistein / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000235:genetics; D006965:Hyperplasia; D007985:Leydig Cells / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000032:analysis; D012670:Seminiferous Epithelium / Q000187:drug effects / Q000473:pathology; D012741:Sexual Maturation; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects*
D016588:Anticarcinogenic Agents; D012333:RNA, Messenger; D004958:Estradiol; D019833:Genistein; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase