Anti-allergic activity of naringenin chalcone from a tomato skin extract.
The anti-allergic activity of a tomato extract was studied by using an in vitro histamine-release assay. The tomato skin extract exerted the strongest inhibition of histamine release. Chlorogenic acid, rutin and naringenin were identified in the 60% ethanol extract of tomato skin. However, the extract contained an unknown compound which strongly inhibited histamine release. This active compound in tomato skin was identified as naringenin chalcone (trans-2'4'6'4-tetrahydroxychalcone). Naringenin chalcone inhibited histamine release with an IC(50) value of 68 microg/ml. The anti-allergic activity of the tomato skin extract was next investigated by the in vivo mouse ear-swelling response. We found that naringenin chalcone showed the strongest inhibitory effect of the polyphenols of the tomato skin extract. These results indicate that a tomato skin extract could inhibit allergic reactions.
Research & Development Division, Kikkoman Corporation, Noda, Chiba 278-0037, Japan. tyamamo@mail.kikkoman.co.jp
Taichi
T
Yamamoto
Mineka
M
Yoshimura
Fumio
F
Yamaguchi
Tomoko
T
Kouchi
Ryohei
R
Tsuji
Makoto
M
Saito
Akio
A
Obata
Mamoru
M
Kikuchi
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D047188:Chalcones; D005419:Flavonoids / Q000737:chemistry; D006636:Histamine Release / Q000187:drug effects*; D006967:Hypersensitivity / Q000188:drug therapy*; D018551:Solanum lycopersicum / Q000737:chemistry*; D051379:Mice; D010636:Phenols / Q000737:chemistry; D059808:Polyphenols
D018926:Anti-Allergic Agents; D047188:Chalcones; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D002599:Chalcone; C027329:naringenin chalcone