The multi-mode polarization modulation spectrometer: part 1: simultaneous detection of absorption, turbidity, and optical activity.
Circular dichroism (CD) is an important spectroscopic technique for monitoring chirality and biological macromolecule conformation. However, during a CD measurement, absorbance, light scattering/turbidity, and fluorescence can also be detected. The simultaneous measurement of these different spectral features for a single sample is the basis of a multi-mode optical spectrometer. This allows time-efficient gathering of complementary information and provides a scheme to ensure that CD measurements are reliable. Aspects of circular polarization differential light scattering, pH, and temperature variation of a protein (antibody) solution are described. A procedure to help ensure that CD measurements are reliable is described.
Section de Pharmacie, Universitéde Genève, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
Tudor
T
Arvinte
Tam T T
TT
Bui
Ali A
AA
Dahab
Barthélemy
B
Demeule
Alex F
AF
Drake
Dhia
D
Elhag
Peter
P
King
D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods; D003627:Data Interpretation, Statistical; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods