Further evidence that epidermal growth factor enhances the intestinal adaptation following small bowel transplantation.
Recent reports indicate that epidermal growth factor (EGF) plays a crucial role for graft adaptation in rat model of small bowel transplantation (SBT). The administration of EGF enhances intestinal cell proliferating rate and the recovery of mucosal structure. However, the effect of EGF on biological functions including glucose absorption in intestinal graft remains to be elucidated. SBT was performed in the two-step procedure. On the first step, intestinal graft (30-cm jejunum) from Brown Norway rats was exteriorized through abdominal wall as a Thiry-Vella loop in recipient Lewis rats for one week. On the second surgery (POD 7), recipient jejunum was replaced orthotopically by the graft, and transplanted rats were treated intraperitoneally with EGF or its vehicle for 3 days. Analyses of histology and biological functions in the graft were done at POD 14. EGF increased both levels of villus height and crypt depth in the graft of transplanted groups. EGF enhanced the glucose absorption as well as the induction of sodium glucose cotransporter 2- to 3-fold in transplanted groups. Further, EGF stimulated the activities of disaccharidase (maltase and sucrase) and the induction of dipeptide cotransporter. These results demonstrate that EGF enhances the structural and functional adaptation of intestinal grafts after SBT. EGF may be useful therapy for patients following intestinal transplantation.
The Second Department of Surgery, Kansai Medical University, Osaka 570-8506, Japan.
Koji
K
Nakai
Yoshinori
Y
Hamada
Yasunori
Y
Kato
Katsuhiko
K
Kitagawa
Koshiro
K
Hioki
Seiji
S
Ito
Tadayoshi
T
Okumura
D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D004187:Disaccharides / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006085:Graft Survival / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects / Q000637:transplantation*; D008562:Membrane Glycoproteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D051269:Sodium-Glucose Transporter 1; D018709:Statistics, Nonparametric
D004187:Disaccharides; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D051269:Sodium-Glucose Transporter 1; D004815:Epidermal Growth Factor; D005947:Glucose