LcoP, an osmoregulated betaine/ectoine uptake system from Corynebacterium glutamicum.
In Corynebacterium glutamicum, four uptake systems for compatible solutes have been characterized so far. DHPE (DeltabetPDeltaputPDeltaproPDeltaectP), a derivative of the C. glutamicum type strain ATCC 13032 carrying deletions in the corresponding genes, still showed a low betaine uptake rate of 1.4 nmol/(min mg cdm). Genome analyses revealed the presence of a putative carrier, named low capacity osmoregulated permease (LcoP), which shows similarities to compatible solute transporters of the betaine/carnitine/choline transporter (BCCT)-family. Deletion of lcoP in DHPE resulted in betaine and ectoine uptake deficiency. LcoP, a betaine and ectoine permease is regulated at the expression and the activity level by the external osmolality. Addition of local anesthetics modulated the activity of BCCT-family members BetP, EctP, and LcoP in a different manner, indicating a different type of lipid-protein interaction.
Institut für Biochemie der Universität zu Köln, Zülpicher Str. 47, 50674 Köln, Germany.
Ralf
R
Steger
Martin
M
Weinand
Reinhard
R
Krämer
Susanne
S
Morbach
D000595:Amino Acid Sequence; D000599:Amino Acids, Diamino / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003352:Corynebacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011392:Proline / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology
D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D001622:Betaine; D012965:Sodium Chloride; D013012:Sorbitol; D012313:RNA; C045628:ectoine; D011392:Proline