Altered CD45 isoform expression affects lymphocyte function in CD45 Tg mice.
Transgenic mice have been constructed expressing high (CD45RABC) and low (CD45R0) molecular weight CD45 isoforms on a CD45-/- background. Phenotypic analysis and in vivo challenge of these mice with influenza and lymphocytic choriomeningitis viruses shows that T cell differentiation and peripheral T cell function are related to the level of CD45 expression but not to which CD45 isoform is expressed. In contrast, B cell differentiation is not restored, irrespective of the level of expression of a single isoform. All CD45 trangenic mice have T cells with an activated phenotype and increased T cell turnover. These effects are more prominent in CD8 than CD4 cells. The transgenic mice share several properties with humans expressing variant CD45 alleles and provide a model to understand immune function in variant individuals.
The Edward Jenner Institute for Vaccine Research, Compton, Berkshire RG20 7NN, UK. elma.tchilian@jenner.ac.uk
Elma Z
EZ
Tchilian
Ritu
R
Dawes
Lisa
L
Hyland
Maria
M
Montoya
Agnes
A
Le Bon
Persephone
P
Borrow
Sam
S
Hou
David
D
Tough
Peter C L
PC
Beverley
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009975:Orthomyxoviridae / Q000276:immunology; D020033:Protein Isoforms; D013601:T-Lymphocytes / Q000502:physiology*
D020033:Protein Isoforms; D017493:Leukocyte Common Antigens