Effect of divergent selection for testosterone production on testicular morphology and daily sperm production in boars.
The objective of this study was to characterize correlated responses in testicular morphology and daily sperm production to divergent selection for testosterone production. Duroc boars from high and low lines (HTL and LTL, respectively) divergently selected over 10 generations for testosterone production in response to a GnRH challenge followed by random selection were used. Testicular tissues were sampled from all available males of generation 20 (HTL, n = 46; and LTL, n = 13). Volume densities for Leydig cells, seminiferous tubules, and Sertoli cells were estimated along with sperm production. The HTL boars had greater volume densities of Leydig cells than did LTL (P < 0.01). Volume density of seminiferous tubules tended to differ between lines (P < 0.07), but Sertoli cell volume densities did not differ (P < 0.27). Sperm production traits, adjusted for age, did not differ significantly between lines. Body, testicular, and epididymal weights were recorded for boars from HTL (n = 82) and LTL (n = 44) from generations 20 and 21. After adjustment for BW, average paired testicular weights for HTL and LTL were 417 and 457 g (P < 0.01), respectively. Epididymal weights, adjusted for BW, were heavier for HTL (P < 0.01) than for LTL. To demonstrate that the selection lines still differed for testosterone production, lines were evaluated in generation 21. Endogenous testosterone production of the HTL (n = 54) and LTL (n = 44) testosterone production line averaged 49.0 ng/mL and 27.8 ng/mL (P < 0.01), respectively. Plasma FSH concentrations did not differ between lines (P < 0.30). Selection for testosterone production in response to a GnRH challenge was an effective method of changing testosterone concentrations, testicular size, epididymal weight, and volume density of Leydig cells. However, daily sperm production per gram of testes was unchanged. Based on the results of this study, selection for testosterone production is not recommended as a method of increasing sperm production in pigs.
Walker
S
S
Department of Animal Science, North Carolina State University, Raleigh 27695-7621, USA.
Robison
O W
OW
Whisnant
C S
CS
Cassady
J P
JP
D000818:Animals; D002452:Cell Count / Q000662:veterinary; D005640:Follicle Stimulating Hormone / Q000097:blood; D007985:Leydig Cells / Q000166:cytology; D008297:Male; D009929:Organ Size; D012641:Selection, Genetic; D012671:Seminiferous Tubules / Q000033:anatomy & histology / Q000502:physiology; D012708:Sertoli Cells / Q000166:cytology; D013076:Sperm Count / Q000662:veterinary; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D013737:Testis / Q000033:anatomy & histology* / Q000166:cytology / Q000502:physiology*; D013739:Testosterone / Q000097:blood* / Q000235:genetics
D013739:Testosterone; D005640:Follicle Stimulating Hormone