Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes.
Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mRNAs and decreased levels of mRNA encoding proteins involved in translation and type III secretion.
Dahan
Sivan
S
Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
Knutton
Stuart
S
Shaw
Robert K
RK
Crepin
Valerie F
VF
Dougan
Gordon
G
Frankel
Gad
G
D000818:Animals; D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000382:microbiology*; D004912:Erythrocytes / Q000382:microbiology; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D018869:Heat-Shock Response; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011817:Rabbits; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins