High pretransplant serum levels of CXCL10/IP-10 are related to increased risk of renal allograft failure.
In experimental models, the chemokine CXCL10/IP-10 is required for graft failure owing to both acute and chronic rejection. In the present study, pretransplantation sera from 316 cadaver kidney graft recipients were tested for serum CXCL10 and CCL22/MDC levels by an ELISA assay. Kidney graft recipients with normally functioning grafts showed significantly lower serum CXCL10 levels than patients who experienced graft failure, whereas no differences for serum CCL22 levels were observed. After the assignment of all patients to four groups according to serum CXCL10 levels, the death-censored survival rates of grafts were 97.5%, 93.6%, 89.7%, 78.7% (p = 0.0006) at 5 years, while no influence was observed on patient survival. Accordingly, patients with the highest CXCL10 levels showed an increased frequency and severity of rejection episodes. Serum C-reactive protein (CRP) level was also assayed in the same samples. Increase of serum CRP levels represented a predictive parameter for death, but not for graft failure. Multivariate analysis demonstrated that among the analyzed variables, CXCL10 had the highest predictive power of graft loss (RR 2.787). Thus, measurement of pretransplant serum CXCL10 levels might represent a clinically useful parameter to identify subjects who are at high risk of severe rejection and graft failure.
Department of Clinical Pathophysiology, University of Florence, Italy.
Mario
M
Rotondi
Alberto
A
Rosati
Andrea
A
Buonamano
Laura
L
Lasagni
Elena
E
Lazzeri
Fabio
F
Pradella
Vittorio
V
Fossombroni
Calogero
C
Cirami
Francesco
F
Liotta
Giorgio
G
La Villa
Mario
M
Serio
Elisabetta
E
Bertoni
Maurizio
M
Salvadori
Paola
P
Romagnani
D000328:Adult; D015415:Biomarkers / Q000097:blood; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000097:blood*; D005260:Female; D006084:Graft Rejection / Q000453:epidemiology; D006085:Graft Survival / Q000502:physiology; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012307:Risk Factors; D017211:Treatment Failure
D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D019743:Chemokines, CXC