Retinol enhances differentiation of the gastric parietal cell lineage in developing rabbits.
In the gastric glands, parietal cells are the targets for anti-ulcer drugs because they contain the proton pump or HK-ATPase responsible for acid secretion. Little is known about factors influencing developmental expression and activity of HK-ATPase. In this study, the parietal cell lineage was investigated in rabbits at post-natal days 0 (P0) to P60 by using morphological and biochemical methods. Immunohistochemical and ultrastructural studies show that the HK-ATPase-expressing cells that appear at P0 and P3 are pre-parietal cells. However, terminally differentiated, mature parietal cells make their appearance at P7. These data correlate with the activity of HK-ATPase, measured as K(+)-dependent hydrolysis of p-nitrophenyl phosphate. Three-day-retinol treatment of P3-P30 rabbits induced an increase in the (i) production of parietal cells, (ii) intensity of the HK-ATPase immunostaining per cell, (iii) activity of HK-ATPase and (iv) amount of HK-ATPase protein measured by Western blotting. In conclusion, retinol upregulates the development of HK-ATPase in rabbits, perhaps due to precocious acceleration of the differentiation program of parietal cell lineage.
Department of Anatomy, Faculty of Medicine & Health Sciences, UAE University, Al-Ain, United Arab Emirates. skaram@uaeu.ac.ae
Sherif M
SM
Karam
Habib R
HR
Ansari
Wafa S
WS
Al-Dhaheri
Glory
G
Alexander
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis / Q000378:metabolism*; D010295:Parietal Cells, Gastric / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D011817:Rabbits; D014801:Vitamin A / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D014801:Vitamin A; D017506:H(+)-K(+)-Exchanging ATPase