Expression and purification of the Bacillus subtilis thioredoxin superfamily protein YkvV.
Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thiol-disulfide oxidoreductase activity.
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Ryoichi
R
Tanaka
Yoko
Y
Araki
Makoto
M
Mizukami
Akira
A
Miyauchi
Matsujiro
M
Ishibashi
Hiroko
H
Tokunaga
Masao
M
Tokunaga
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013879:Thioredoxins / Q000378:metabolism*
D013879:Thioredoxins