A 'one-pot' assay for the accessibility of DNA in a nucleosome core particle.
The accessibility of nucleosomal DNA to transcription factors and other sequence-specific DNA binding proteins is of importance in the consideration of mechanisms of transcriptional control. Here, we report a simple novel assay which determines this accessibility at eight different rotationally equivalent sites on nucleosomal DNA and shows that linker histones and the chromosomal HMGB proteins, HMG-D and HMG-Z, have opposite effects on the accessibility of nucleosomal DNA. We compare this assay to previously described methods.
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Chenyi
C
Wu
Andrew
A
Travers
D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D007700:Kinetics; D023282:Nuclease Protection Assays / Q000379:methods*; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism*
D006609:High Mobility Group Proteins; D006657:Histones; D009707:Nucleosomes; D004247:DNA; D004262:DNA Restriction Enzymes