In vivo radioprotection of mice by 3-methyl-1-phenyl-2-pyrazolin-5-one (edaravone; Radicut), a clinical drug.
Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one; Radicut) is a brain-protecting agent used clinically to treat acute ischemic stroke with a reaction mechanism of free radical scavenging. Since the initial stage of radiation damage involves the formation of free radicals, edaravone is expected to be effective in preventing lethal damage from ionizing radiation. In the present study, we used mice to examine in vivo the radioprotective effect of edaravone on whole body X-ray irradiation. A solution of edaravone was administered intraperitoneally to C3H mice (male, 10 weeks old), and they were irradiated with a total dose of 8.0 Gy. Edaravone exhibited dose-dependent and injection time-dependent radioprotection. When injected 30 min before the X-ray irradiation, it had the greatest radioprotective effect, whereas an injection after the irradiation showed no protective effect. The LD(50/30) was about 8.8 Gy for edaravone-injected mice and 6.6 Gy for control mice, yielding a DRF for edaravone (450 mg/kg bw) of 1.3. Edaravone decreased the body temperature transiently about 3-6C, but this did not seem to be responsible for the radioprotection. Since the radioprotection was observed only when the reagent was administered before the irradiation, the primary action of edaravone might be the quenching of free radicals with a short lifetime generated by the irradiation.
Redox Regulation Research Group, National Institute of Radiological Sciences, Chiba, Japan. anzai@nirs.go.jp
Kazunori
K
Anzai
Masako
M
Furuse
Akira
A
Yoshida
Azusa
A
Matsuyama
Takashi
T
Moritake
Koji
K
Tsuboi
Nobuo
N
Ikota
D000818:Animals; D000983:Antipyrine / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D001831:Body Temperature / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D000077553:Edaravone; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D004364:Pharmaceutical Preparations / Q000008:administration & dosage; D011832:Radiation Injuries / Q000209:etiology* / Q000517:prevention & control*; D011835:Radiation Protection / Q000379:methods*; D011836:Radiation Tolerance / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D013534:Survival; D016019:Survival Analysis; D016896:Treatment Outcome; D014916:Whole-Body Irradiation; D014965:X-Rays
D004364:Pharmaceutical Preparations; D011837:Radiation-Protective Agents; D000077553:Edaravone; D000983:Antipyrine