Differences in the inheritance stability of kanamycin resistance between transgenic cucumbers (Cucumis sativus L.) containing two constructs.
A phenotypic segregation of kanamycin resistance conferred by nosnptII was investigated in two groups of transgenic cucumber lines one containing the PR-2duidA-nosnptII construct and the other with the thaumatinII-nosnptII construct to the third and fifth generation, respectively. Expression of the nptII gene was more stable and predictable in the progeny of the PR-2d lines than that of thaumatin lines. In T2 and T3 generations representing seven PR-2d primary transformants, 78% of the hemizygous progenies exhibited a segregation ratio consistent with Mendelian inheritance; non-Mendelian ratio occurred in the remaining 22%. Mendelian segregation was observed in 46% of the hemizygous progenies derived from 11 thaumatin primary transformants. The segregation ratio for two and three independent loci appeared in single PR-2d and thaumatin lines, respectively.
Yin
Zhimin
Z
Institute of Plant Genetics, Polish Academy of Science, 60-479 Poznań, Poland.
Szwacka
Maria
M
Malinowski
Robert
R
Malepszy
Stefan
S
D003433:Crosses, Genetic; D018553:Cucumis sativus / Q000235:genetics*; D018014:Gene Transfer Techniques; D007613:Kanamycin Resistance / Q000235:genetics*; D008957:Models, Genetic; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology*; D036226:Seedlings / Q000235:genetics
D010940:Plant Proteins; C003427:thaumatin protein, plant