Vif is an auxiliary factor of the HIV-1 reverse transcriptase and facilitates abasic site bypass.
The HIV-1 accessory protein Vif was found to modulate the RNA- and DNA-dependent DNA synthesis activity of the viral RT (reverse transcriptase) in two ways: (i) it stimulated the binding of the viral RT to the primer by increasing the association rate kcat/K(m) and by decreasing the thermodynamic barrier DeltaH([ES]) for complex formation, and (ii) it increased the polymerization rate of HIV-1 RT. A Vif mutant lacking the final 56 amino acids at the C-terminus failed to stimulate the viral RT. On the other hand, another Vif mutant lacking the first 43 amino acids at the N-terminus, which are involved in RNA binding and interaction with the viral protease, was able to stimulate RT activity. In addition, Vif was found to promote the bypass of an abasic site by HIV-1 RT.
Cancio
Reynel
R
Istituto di Genetica Molecolare IGM - CNR, via Abbiategrasso 207, I-27100 Pavia, Italy.
Spadari
Silvio
S
Maga
Giovanni
G
D017931:DNA Primers / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D016346:Gene Products, vif / Q000502:physiology*; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D009696:Nucleic Acids / Q000378:metabolism; D010455:Peptides / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012191:Retroviridae Proteins / Q000502:physiology; D013379:Substrate Specificity; D013698:Templates, Genetic; D013816:Thermodynamics; D054320:vif Gene Products, Human Immunodeficiency Virus
D017931:DNA Primers; D004279:DNA, Viral; D016346:Gene Products, vif; D009696:Nucleic Acids; D010455:Peptides; D011687:Purines; D011743:Pyrimidines; D012367:RNA, Viral; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; D054320:vif Gene Products, Human Immunodeficiency Virus; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases