GluR4c, an alternative splicing isoform of GluR4, is abundantly expressed in the adult human brain.
We report the cloning of human GluR4c, an alternative splicing isoform of GluR4. Similar to rodent and chick GluR4c mRNA, human GluR4c had a 113-bp insert containing a stop codon, resulting in a short C terminus. The expression of human GluR4c was widespread in the brain, and was upregulated with development in the cerebellum and cerebral cortex where the level of it was about 30% of total GluR4 mRNA in adult stage. The GluR4 subunit may play a pivotal role in regulating channel properties as well as trafficking of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors in the adult human brain.
Department of Neurology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan.
Yukio
Y
Kawahara
Kyoko
K
Ito
Hui
H
Sun
Masayuki
M
Ito
Ichiro
I
Kanazawa
Shin
S
Kwak
D000328:Adult; D000368:Aged; D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D002645:Chickens; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D011180:Postmortem Changes; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods
D020033:Protein Isoforms; D012333:RNA, Messenger; D018091:Receptors, AMPA; C104724:glutamate receptor ionotropic, AMPA 4