Sugar utilization and anoxia tolerance in rice roots acclimated by hypoxic pretreatment.
Although most cereal roots cannot elongate under anoxic conditions, primary roots of three-day-old rice (Oryza sativa L.) seedlings were able to elongate during a 24-h period of anoxia. Hypoxic pretreatment (H-PT) increased the elongation of their roots. Sucrose synthase (EC 2.4.1.13), glucokinase (EC 2.7.1.2), fructokinase (EC 2.7.1.4), pyruvate decarboxylase (EC 4.1.1.1) and alcohol dehydrogenase (EC 1.1.1.1) activities were increased by anoxia in both H-PT and non-pretreated (N-PT) roots. However, these activities were greater in the H-PT roots than in the N-PT roots. The average rate of production of ethanol for the initial 6h after the onset of anoxia was 3.7 and 1.4 micromolg(-1) fresh weight h(-1) for the H-PT and N-PT roots, respectively, suggesting that ethanolic fermentation may increase more quickly in the H-PT roots than in the N-PT roots. Roots of the seedlings lost ATP and total adenine nucleotides in anoxia, however, the H-PT roots maintained higher levels of ATP and total adenine nucleotides compared to the N-PT roots. These results show that rice roots are able to utilize the set of enzymes involved in the metabolism of soluble sugars under anoxia. The ability to maintain an active fermentative metabolism for production of ATP by fueling the glycolytic pathway with fermentable carbohydrate is probably greater in H-PT than in N-PT roots.
Faculty of Agriculture, Department of Biochemistry and Food Science, Kagawa University, Miki, Kagawa, Japan. hisashi@ag.kagawa-u.ac.jp
Hisashi
H
Kato-Noguchi
D000064:Acclimatization / Q000187:drug effects / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D050260:Carbohydrate Metabolism; D000431:Ethanol / Q000378:metabolism; D005631:Fructokinases / Q000378:metabolism; D005941:Glucokinase / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D011767:Pyruvate Decarboxylase / Q000378:metabolism
D000431:Ethanol; D000255:Adenosine Triphosphate; D000426:Alcohol Dehydrogenase; D005964:Glucosyltransferases; C022699:sucrose synthase; D005631:Fructokinases; D005941:Glucokinase; C046173:fructokinase; D011767:Pyruvate Decarboxylase; D010100:Oxygen