Bcl-2 overexpression does not promote axonal regeneration of the entorhino-hippocampal connections in vitro after axotomy.
CNS lesions trigger cell death in injured neurons and glia. Genes of the bcl-2 family play crucial roles in the control of apoptosis and cell survival in the CNS. Recently, it has been suggested that overexpression of bcl-2 induces axonal elongation and regeneration in vitro and in vivo. Here, we analyze the regenerative potential of bcl-2 overexpression in the axotomized entorhino-hippocampal connection in organotypic slice cocultures. Our results show that in slice cocultures from bcl-2-overexpressing mice, there is a decrease in the number of dead neurons in the entorhinal cortex. In addition, axonal regeneration is not enhanced after axotomy. Thus, in the entorhino-hippocampal formation in vitro, bcl-2 overexpression rescues neurons from axotomy-induced cell death but fails to enhance the regeneration of the entorhino-hippocampal connection.
Development and Regeneration of the CNS, Department of Cell Biology, I.R.B.B.-P.C.B., Science Park of Barcelona, University of Barcelona, Josep Samitier 1-5, E-08028 Barcelona, Spain.
Marta
M
Solé
Xavier
X
Fontana
Rosalina
R
Gavín
Eduardo
E
Soriano
José Antonio
JA
del Río
D000818:Animals; D001369:Axons / Q000378:metabolism*; D019771:Axotomy; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D018920:Coculture Techniques; D019938:Cyclin D1 / Q000378:metabolism*; D018728:Entorhinal Cortex / Q000166:cytology / Q000378:metabolism*; D020439:Growth Cones / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009416:Nerve Regeneration / Q000502:physiology*; D009924:Organ Culture Techniques; D019580:Perforant Pathway / Q000254:growth & development* / Q000378:metabolism
D019938:Cyclin D1