Lipid-mediated light activation of a mechanosensitive channel of large conductance.
This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-[[4-(4-butylphenyl)azo]phenoxy]pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL.
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Joost H A
JH
Folgering
Johanna M
JM
Kuiper
Alex H
AH
de Vries
Jan B F N
JB
Engberts
Bert
B
Poolman
D001391:Azo Compounds / Q000737:chemistry / Q000528:radiation effects; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology / Q000528:radiation effects; D004926:Escherichia coli / Q000737:chemistry / Q000502:physiology; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology / Q000528:radiation effects*; D013294:Lactococcus lactis / Q000737:chemistry / Q000502:physiology; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry / Q000528:radiation effects; D008055:Lipids / Q000737:chemistry* / Q000528:radiation effects*; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000528:radiation effects; D010713:Phosphatidylcholines / Q000737:chemistry / Q000528:radiation effects; D013237:Stereoisomerism; D013499:Surface Properties; D013997:Time Factors
D001391:Azo Compounds; D001426:Bacterial Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008055:Lipids; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; C510725:di-(5-((4-(4-butylphenyl)azo)phenoxy)pentyl)phosphate; C017251:1,2-oleoylphosphatidylcholine