Acute phase response in reindeer after challenge with Escherichia coli endotoxin.
The serum concentrations of two acute phase proteins (APPs), haptoglobin (Hp) and serum amyloid-A (SAA), were monitored in reindeer after challenge with endotoxin. Four adult female reindeer received either 0.1 microg/kg Escherichia coli 0111:B4 lipopolysaccharide B or saline solution intravenously. At the second challenge, the treatments were reversed. In addition to the APPs, changes in blood chemistry and rectal temperature were monitored. The endotoxin challenge caused a significant increase in SAA (peak 48 h) and a sharp decrease (8-12 h) of serum iron concentrations in all animals. The mean Hp concentration increased at 8 h and remained elevated until 48 h, but no statistically significant differences were found. This investigation demonstrates that challenge with a single-bolus dose of E. coli endotoxin can activate the acute phase response (APR) and SAA appears to be a more sensitive indicator of the APR than Hp during bacterial infection in reindeer.
Orro
T
T
Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, P.O. BOX 57, 00014 Helsinki, Finland. toomas.orro@helsinki.fi
Sankari
S
S
Pudas
T
T
Oksanen
A
A
Soveri
T
T
D000210:Acute-Phase Reaction / Q000097:blood / Q000276:immunology*; D000818:Animals; D019446:Endotoxemia / Q000097:blood / Q000276:immunology; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D007501:Iron / Q000097:blood; D012053:Reindeer / Q000276:immunology*; D000685:Serum Amyloid A Protein / Q000378:metabolism
D004731:Endotoxins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; C044101:endotoxin, Escherichia coli; D007501:Iron