Poly(N-isopropylacrylamide)-grafted gelatin as a thermoresponsive cell-adhesive, mold-releasable material for shape-engineered tissues.
Poly(N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin) can be used as a thermoresponsive cell-adhesive matrix and mold-releasable material for shape-engineered tissues. An example of such a tissue with predetermined shape is realized by fabrication of a tubular endothelial cell construct. A solution containing a mixture of PNIPAM-gelatin and PNIPAM was coated on the luminal surface of a glass capillary tube. After air-drying, endothelial cells were seeded and cultured for four days at 37 degrees C. The infusion of the culture medium into the tube at 20 degrees C resulted in the spontaneous detachment and removal of a tubular vascular tissue composed of endothelial cells and supramolecularly organized extracellular matrices produced by endothelial cells on the basal side of the tissue. The potential use of a thermoresponsive cell-adhesive and mold-releasable material in complex-shaped tissue-engineered devices is discussed.
Division of Biomedical Engineering, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka, Japan. matsuda@med.kyushu-u.ac.jp
Takehisa
T
Matsuda
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials; D001709:Biotechnology; D002196:Capillaries; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology; D005780:Gelatin / Q000737:chemistry*; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D008954:Models, Biological; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D003470:Culture Media; C052970:poly-N-isopropylacrylamide; D005780:Gelatin