RHD 1227A is an important genetic marker for RhD(el) individuals.
Approximately 30% of apparently Rh- Taiwanese people actually were RhD(el), a rare variant of the Rh system that might carry a grossly intact RHD gene. Several studies have indicated that the RhD(el) trait might be generated by multiple molecular mechanisms. In this study, a total of 294 Taiwanese serologically RhD- blood donors were tested for Rh phenotypes and RHD genotypes. Among them, total RHD deletion, partial RHD gene, and RhD(el) were found in 185 (62.9%), 15 (5.1%), and 94 (32.0%), respectively. The 1227A allele and exon 9 of the RHD gene were found in all 94 RhD(el) donors. The Ccee was the most prevalent phenotype in the RhD(el) group (78/94 [83%]), and the ccee phenotype was highly prevalent in the true D- group (87.6%). RHD 1227A can be used as an important and useful genetic marker for RhD(el). It can be detected easily by a simple, rapid, specific sequence primer-polymerase chain reaction method.
Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan.
Jung-Chin
JC
Chen
Tsun-Mei
TM
Lin
Yi Lin
YL
Chen
Ya-Hui
YH
Wang
Ying-Tai
YT
Jin
Chung-Tai
CT
Yue
D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D017931:DNA Primers; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D013624:Taiwan
D017931:DNA Primers; D005819:Genetic Markers; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen