Determination of ochratoxin A in meat products by high-performance liquid chromatography coupled to electrospray ionisation sequential mass spectrometry.
A method based on liquid chromatography with electrospray ionisation ion trap mass spectrometry, for the determination of ochratoxin A (OTA) in meat products using ochratoxin B (OTB) as an internal standard, is described. Fragmentation patterns of OTA and OTB were studied by sequential mass spectrometry. Trace determination was then accomplished by consecutive reaction monitoring (CRM) of a fragment obtained by MS(3) experiments. This led to a better signal-to-noise ratio and to a higher specificity of the technique. The response to OTA was linear over at least one concentration decade with a limit of detection of 0.6 ng/g. The method was applied to pig tissue samples naturally contaminated by OTA.
Dipartimento di Chimica, Università di Bari, Via E. Orabona 4, 70126 Bari, Italy.
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I
Losito
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L
Monaci
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F
Palmisano
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Tantillo
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A