Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes.
Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mRNAs and decreased levels of mRNA encoding proteins involved in translation and type III secretion.
Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom.
Sivan
S
Dahan
Stuart
S
Knutton
Robert K
RK
Shaw
Valerie F
VF
Crepin
Gordon
G
Dougan
Gad
G
Frankel
D000818:Animals; D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000382:microbiology*; D004912:Erythrocytes / Q000382:microbiology; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D018869:Heat-Shock Response; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011817:Rabbits; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins