Cytokine removal with a novel adsorbent polymer.
OBJECTIVE
We sought to characterize a novel adsorbent polymer in terms of cytokine removal.


METHODS
We challenged 50 rats with lipopolysaccharide to obtain cytokine-rich blood and circulated this through cartridges containing polymer. In separate experiments, cell-free supernatants were passed through cartridges containing polymer. We measured tumor necrosis factor alpha, interleukin 10 and interleukin 6 concentrations under a variety of conditions to evaluate adsorption kinetics.


RESULTS
All three cytokines were rapidly removed from the blood with less than 50% of the initial concentrations present after 1 h of circulation through the cartridge. There was no significant difference in the effect across a range of blood flows and Ca2+ concentrations. Adsorption was decreased somewhat by extremely low temperature (4 degrees C).


CONCLUSIONS
The adsorbent polymer removes cytokines with high efficiency, and binding is relatively unaffected by a variety of physical conditions.
The MANTRA Laboratories, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
Mingchen
M
Song
James
J
Winchester
Robert L
RL
Albright
Vincent J
VJ
Capponi
Michael D
MD
Choquette
John A
JA
Kellum
D000818:Animals; D002118:Calcium; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000737:chemistry; D016207:Cytokines / Q000097:blood / Q000302:isolation & purification*; D016753:Interleukin-10 / Q000097:blood / Q000302:isolation & purification; D015850:Interleukin-6 / Q000097:blood / Q000302:isolation & purification; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology; D008297:Male; D011108:Polymers / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D016060:Sorption Detoxification / Q000379:methods*; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha / Q000302:isolation & purification
D017077:Culture Media, Conditioned; D016207:Cytokines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D002118:Calcium