[The induced apoptosis of recombinant adenovirus carrying melittin gene for hepatocellular carcinoma cell].
OBJECTIVE
To observe the induced apoptosis of recombinant adenovirus carrying melittin gene (Ad-rAFP-Mel) for hepatocellular carcinoma cell line (BEL-7402).


METHODS
The morphological observe, DNA electrophoresis, TUNEL and Flow cytometry assay were used to study the apoptosis of BEL-7042 cell line transfected by Ad-rAFP-Mel.


RESULTS
The morphological changes and apoptosis of BEL-7402 transfected by Ad-rAFP-Mel were confirmed with microscopy and DNA electrophoresis, TUNEL, Flow cytometry assay. The DNA ladder could be demonstrated on DNA electrophoresis in Ad-rAFP-Mel group. The apoptosis rates of BEL-7402 cells in Ad-rAFP-Mel, Ad-rAFP, and control groups were (21.5+/-2.4)%, (10.5+/-4.4)% and (3.0+/-1.4)% respectively by TUNEL assay (F = 38.0, P < 0.05) and were (7.3+/-0.5)%, (3.9+/-0.1)% and (0.8+/-0.1)% respectively by flow cytometry assay (F = 415.1, P < 0.05).


CONCLUSIONS
It seems that melittin inducing apoptosis might be one of the antitumor mechanisms.
Department of Chinese Traditional Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Bai
B
Li
Chang-quan
CQ
Ling
Chen
C
Zhang
Wei
W
Gu
Shao-xiang
SX
Li
Xue-qiang
XQ
Huang
Ya-ni
YN
Zhang
Chao-qin
CQ
Yu
D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008555:Melitten / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D011993:Recombinant Fusion Proteins; D008555:Melitten