Surface-activated chemical ionization ion trap mass spectrometry in the analysis of amphetamines in diluted urine samples.
A new ionization method, named surface-activated chemical ionization (SACI), was employed for the analysis of five amphetamines (3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDE), amphetamine and methamphetamine) by ion trap mass spectrometry. The results so obtained have been compared with those achieved by using atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) using the same instrument, clearly showing that SACI is the most sensitive of the three. The limit of detection and linearity range for SACI were compared with those obtained using APCI and ESI, showing that the new SACI approach provides the best results for both criteria. SACI was used to analyze MDA, MDMA MDE, amphetamine and methamphetamine in four urine samples, and the quantitation results are compared with those achieved using ESI.
University of Milan, Centre for Bio-molecular Interdisciplinary Studies and Industrial Applications (CISI), Via Fratelli Cervi 93, 20090 Segrate Milano, Italy. dtoycr@tin.it
Simone
S
Cristoni
Luigi Rossi
LR
Bernardi
Piermario
P
Gerthoux
Elisabetta
E
Gonella
Paolo
P
Mocarelli
D000662:Amphetamines / Q000652:urine*; D001274:Atmospheric Pressure; D000697:Central Nervous System Stimulants / Q000652:urine*; D006801:Humans; D007477:Ions; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*
D000662:Amphetamines; D000697:Central Nervous System Stimulants; D007477:Ions