Deodorization with ku-ding-cha containing a large amount of caffeoyl quinic acid derivatives.
Caffeoyl quinic acid (CQA) derivatives in ku-ding-cha, mate, coffee, and related plants were determined by HPLC. One ku-ding-cha contained a large amount of 3,5-dicaffeoylquinic acid (3,5-diCQA, 10.6% in dry weight) as well as 3-CQA (1.7%), 4-CQA (1.1%), 5-CQA (6.3%), 3,4-diCQA (1.8%), and 4,5-diCQA (4.3%). In this ku-ding-cha, the total caffeic acid moiety was 90.3 mmol/100 g of dry weight. The leaves of Ilex latifolia, which is one original species of ku-ding-cha, and another plant of the same genus, I. rotunda, also contained 3,5-diCQA (9.5 and 14.6%), 3-CQA (4.3 and 1.9%), and 5-CQA (4.8 and 3.8%), respectively, whereas raw coffee bean contained 5.5% 5-CQA and other low CQA derivatives. 3,5-DiCQA and 5-CQA with an apple acetone powder (AP) containing polyphenol oxidase showed high capturing activities toward thiols, and two addition compounds between 3,5-diCQA and methane thiol were also identified. Ku-ding-cha indicated extremely strong capturing activities toward methanethiol, propanethiol, and 2-propenethiol in the presence of apple AP. Furthermore, drinking ku-ding-cha reduced the amount of allyl methyl sulfide gas, well-known to persist as malodorous breath long after the ingestion of garlic.
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. negishi@sakura.cc.tsukuba.ac.jp
Osamu
O
Negishi
Yukiko
Y
Negishi
Fumiyoshi
F
Yamaguchi
Tatsuyuki
T
Sugahara
D000498:Allyl Compounds / Q000737:chemistry; D001628:Beverages / Q000032:analysis; D028241:Camellia sinensis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D040503:Coffea / Q000737:chemistry; D003836:Deodorants / Q000737:chemistry*; D031659:Ligustrum / Q000737:chemistry*; D027845:Malus / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry
D000498:Allyl Compounds; D003836:Deodorants; D013438:Sulfhydryl Compounds; D013440:Sulfides; C472707:caffeoylquinic acid; D011801:Quinic Acid; C077207:allyl methyl sulfide