Toxicological evaluation of bacterial decolourised anaerobically treated distillery effluent with common duckweed (Lemna minor).
Duckweed (Lemna minor) a small vascular plant, grows rapidly, is sensitive to a wide variety of toxicants and is easy to culture. A method is described that measures duckweed frond growth, chlorophyll, protein and biomass content as indicator of growth inhibition. The physico-chemical analysis of anaerobically treated distillery effluent revealed high BOD (28,000 mg/l), COD (52,400 mg/l) and dark brown colour (180,000 Co. Pt.). This effluent showed high toxicity to Lemna minor after 96 h of exposure in laboratory condition. EC50 of the fronds for chlorophyll, protein and biomass was found to be 25%, however, the bacterial decolourised effluent showed reduction of BOD (87.50%), COD (84.50%) and colour (76%). Further the toxicity evaluation with Lemna minor showed toxicity reduction up to 63% for all tested parameters. The EC50 noted for chlorophyll, protein and biomass was 100% concentration of decolourised effluent.
Environmental Microbiology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow-226 001, UP, India.
Ram
R
Chandra
Archana
A
Srivastava
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001681:Biological Assay; D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D007218:Industrial Microbiology; D007220:Industrial Waste; D010860:Pigments, Biological / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000187:drug effects*; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods*; D014873:Water Pollutants / Q000633:toxicity*
D007220:Industrial Waste; D010860:Pigments, Biological; D014873:Water Pollutants; D002734:Chlorophyll