Control of glycolysis in mature boar spermatozoa: effect of pH in vitro.
The glycolytic pathway in boar sperm is sensitive to pH, which decreases as lactate is produced from either glucose or fructose in vitro. The build up of lactate appears to be due to the saturation of mitochondrial lactate transporters, which causes the cytoplasmic pH to fall. Phosphofructokinase has been shown to be sensitive to this drop in pH rather than to the build up of lactate ions or ATP, thereby controlling the rate of glycolysis in vitro.
School of Molecular and Microbial Biosciences, Discipline of Biochemistry, The University of Sydney, Sydney, NSW, Australia. a.jones@mmb.usyd.edu.au
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D000818:Animals; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D008297:Male; D025221:Phosphofructokinases / Q000378:metabolism; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
D019344:Lactic Acid; D025221:Phosphofructokinases; D005947:Glucose