Optimizing RNA extraction yield from whole blood for microarray gene expression analysis.
OBJECTIVE
Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications.


METHODS
We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip.


CONCLUSIONS
Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
Vascular Biology Research Group and London Regional Genomics Centre, Robarts Research Institute and University of Western Ontario, London, ON, N6A 5K8, Canada.
Jian
J
Wang
John F
JF
Robinson
Hafiz M R
HM
Khan
David E
DE
Carter
James
J
McKinney
Brooke A
BA
Miskie
Robert A
RA
Hegele
D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D012313:RNA / Q000097:blood* / Q000235:genetics*
D012313:RNA