Alteration in the composition of cholesteryl glucosides and other lipids in Helicobacter pylori undergoing morphological change from spiral to coccoid form.
In this analysis of membrane lipid compositions in Helicobacter pylori, the membrane lipid profiles drastically changed during coccoid formation: cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside levels increased, cholesteryl-alpha-D-glucopyranoside and phosphatidyl ethanolamine decreased, and a coordinated increase in cardiolipin and decrease in phosphatidyl glycerol were observed. Cholesteryl-6-O-phosphatidyl-alpha-D-glucopyranoside was hardly detected in the spiral forms in the logarithmic phase, but subsequently increased throughout the coccoid conversion. These results suggest that environmental stresses induce the expression of certain regulatory systems for lipid metabolism in H. pylori, and that the resulting alterations in lipid composition play an important role in inducing the coccoid conversion.
Shimomura
Hirofumi
H
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical School, 3311-1, Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan. shimo@jichi.ac.jp
Hayashi
Shunji
S
Yokota
Kenji
K
Oguma
Keiji
K
Hirai
Yoshikazu
Y
D000332:Aerobiosis; D000693:Anaerobiosis; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D016480:Helicobacter pylori / Q000737:chemistry* / Q000166:cytology* / Q000254:growth & development; D007700:Kinetics; D008563:Membrane Lipids / Q000737:chemistry*
D008563:Membrane Lipids; C027578:cholesteryl glucoside; D002784:Cholesterol