The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure.
We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pre-transfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.
Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Tempakucho, Toyohashi, Aichi 441-8580, Japan.
Tomoaki
T
Ando
Terumichi
T
Tanaka
Yo
Y
Kikuchi
D001483:Base Sequence; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020871:RNA Stability / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D014443:Tyrosine / Q000378:metabolism
D014443:Tyrosine; D012343:RNA, Transfer