Dopamine as a robust anchor to immobilize functional molecules on the iron oxide shell of magnetic nanoparticles.
We report on the use of dopamine (DA) as a robust molecular anchor to link functional molecules to the iron oxide shell of magnetic nanoparticles. Using nitrilotriacetic acid (NTA) as the functional molecule, we created a system with an M/Fe2O3-DA-NTA (M = Co or SmCo5.2) nanostructure, which possesses high stability and specificity for separating histidine-tagged proteins. The well-established biocompatibility of iron oxide and the robust covalent bonds between DA and Fe2O3 render this strategy attractive for constructing biofunctional magnetic nanoparticles containing iron oxide.
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Chenjie
C
Xu
Keming
K
Xu
Hongwei
H
Gu
Rongkun
R
Zheng
Hui
H
Liu
Xixiang
X
Zhang
Zhihong
Z
Guo
Bing
B
Xu
D004298:Dopamine / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D006639:Histidine / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D008164:Luminescent Proteins / Q000302:isolation & purification; D008280:Magnetics; D036103:Nanotechnology; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D010316:Particle Size; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D005290:Ferric Compounds; D007093:Imidazoles; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D006639:Histidine; C029899:imidazole; D009571:Nitrilotriacetic Acid; D004298:Dopamine