Immunosuppressive activity of a molecule isolated from Artemisia annua on DTH responses compared with cyclosporin A.
Artemisia belongs to the family of Compositea; there are different Artemisias in Iran, of which Artemisia annua L. is grown in the north of Iran. In this study, Artemisinin was extracted and purified from the whole plants. The purification of Artemisinin was performed using column chromatography in different polarities of solvents and the results were evaluated by Thin Layer Chromatography (TLC). (1)H-NMR (NMR-500) spectroscopy was used to characterize the purified Artemisinin. The immunosuppressive activity of Artemisinin was investigated on Balb/c mice by DTH response in comparison to cyclosporin A (CsA). The data indicated that Artemisinin could suppress the delayed type hypersensitivity (DTH) against sheep blood capsule in Balb/c mice. Also its inhibitory effect on calmodulin (CaM) structure was determined by fluorescence spectroscopy. The data indicated an inhibitory effect of that on the activity of calmodulin by increasing the fluorescence emission of calmodulin. Both in vivo (DTH response) and in vitro (spectrofluorometry) studies indicated the activity of Artemisinin as an immunosuppressive agent and that the fluorescence emission of calmodulin is more than cyclosporin A.
Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
Shokoofeh
S
Noori
Gholam-Ali
GA
Naderi
Zuhair M
ZM
Hassan
Zohre
Z
Habibi
S Zahra
SZ
Bathaie
S Mahmoud M
SM
Hashemi
D000818:Animals; D031172:Artemisia annua / Q000737:chemistry; D037621:Artemisinins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D057927:Hydrophobic and Hydrophilic Interactions; D006968:Hypersensitivity, Delayed / Q000188:drug therapy / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018514:Plant Structures; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D037621:Artemisinins; D002147:Calmodulin; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; D016572:Cyclosporine; C031327:artemisinin