A naturally occurring variation in the proline-rich region does not attenuate human immunodeficiency virus type 1 nef function.
We analyzed human immunodeficiency virus type 1 (HIV-1) Nef variants to further evaluate the functional relevance of the R71T substitution previously proposed to attenuate viral replication (Fackler et al., Curr. Biol. 11:1294-1299, 2001). Our results demonstrate that this variation in the proline-rich region does not significantly affect the functional activity of Nef or HIV-1 infectivity or replication.
Department of Virology-Universitätsklinikum, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Elke
E
Rücker
Jan
J
Münch
Steffen
S
Wildum
Matthias
M
Brenner
Jutta
J
Eisemann
Leonid
L
Margolis
Frank
F
Kirchhoff
D019943:Amino Acid Substitution; D015693:Gene Products, nef / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D015754:Genes, nef; D014644:Genetic Variation; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D016297:Mutagenesis, Site-Directed; D011392:Proline / Q000737:chemistry; D014774:Virulence / Q000235:genetics; D014779:Virus Replication / Q000235:genetics; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus; D011392:Proline