Non-enzymatic platelet-activating factor formation by acetylated proteins.
Substantial amounts of platelet-activating factor (PAF 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine), the potent phospholipid mediator of allergic and inflammatory reactions, are formed upon incubation of acetylated low-density lipoprotein, acetylated bovine serum albumin (BSA) and acetylated apolipoprotein A-I with 1-0-hexadecyl-sn-glycero-3-phosphocholine (lyso-PAF). Acetylated BSA produced 0.3 nmol PAF/mg of protein after a 6 h incubation period with 40 microM lyso-PAF. The transfer of acetate bound to acetylated proteins to lyso-PAF was non-enzymatic. Chemical PAF formation by acetylated proteins, involved in lipid metabolism and transport, could lead to complication of inflammatory and allergic events.
Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina 45110, Greece.
Paraskevi G
PG
Thanou
Demokritos C
DC
Tsoukatos
D000107:Acetylation; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry; D001792:Blood Platelets / Q000737:chemistry; D002413:Cations, Divalent; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000737:chemistry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013696:Temperature
D016632:Apolipoprotein A-I; D002413:Cations, Divalent; D008077:Lipoproteins, LDL; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D012710:Serum Albumin, Bovine