Interleukin 10 genotype as a risk factor for sudden infant death syndrome: determination of IL-10 genotype from wax-embedded postmortem samples.
In a previous study an association was shown between SIDS and an interleukin-10 (IL-10) genotype. That study was carried out on frozen, unfixed tissue samples, but these are difficult to obtain. Fixed samples used for pathological examination are available. The purpose of this study was to extend the previous work by establishing methods to extract and genotype DNA from fixed, wax-embedded tissues specimens and to use the results to seek confirmation of the association between IL-10 genotype and SIDS in a larger collection of SIDS babies. Using an amplification refractory mutation system-polymerase chain reaction method, a total of 38 infants were genotyped for IL-10 alleles and compared with controls. There was a significant association between the IL-10 -592*A allele and SIDS, consistent with the earlier findings. This study lends support to the hypothesis that IL-10 genotype is related to the susceptibility of babies to SIDS.
Korachi
May
M
Oral Microbiology Laboratory, University Dental Hospital of Manchester, Higher Cambridge Street, Manchester M15 6FH, UK.
Pravica
Vera
V
Barson
Anthony J
AJ
Hutchinson
Ian V
IV
Drucker
David B
DB
D001344:Autopsy; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D013398:Sudden Infant Death / Q000235:genetics*; D016610:Tissue Embedding / Q000379:methods; D014885:Waxes
D014885:Waxes; D016753:Interleukin-10