Utility of the Y-STR typing systems Y-PLEX 6 and Y-PLEX 5 in forensic casework and 11 Y-STR haplotype database for three major population groups in the United States.
The Y-PLEX 6 and Y-PLEX 5 systems enable analysis for 11 Y-STR loci. We present here the utility of these systems in forensic casework. A total of 188 samples, including 127 evidence samples, were analyzed using either or both of the systems. The evidence sample types included fingernail scrapings, sperm or seminal fluid, epithelial cells, blood and other tissues. The Y-STR typing systems provided useful probative results in difficult cases. A reference database for Caucasian (n = 517), African American (n = 535), and Hispanic (n = 245) population groups within the United States was generated for estimating the haplotype frequency in forensic casework. Among the individuals profiled, 311 Caucasians, 412 African Americans, and 194 Hispanics provided unique profiles in their respective population datasets. This is the first report describing the haplotype database for the set of 11 Y-STR loci recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Linkage analysis reveals that the frequencies from forensically important autosomal loci can be multiplied with the Y-STR haplotype frequency. The results from Y-PLEX systems have been accepted in courts in the United States.
Sinha
Sudhir K
SK
ReliaGene Technologies, Inc, New Orleans, LA 70123, USA. sinha@reliagene.com
Budowle
Bruce
B
Chakraborty
Ranajit
R
Paunovic
Ana
A
Guidry
Robin DeVille
RD
Larsen
Chris
C
Lal
Amrita
A
Shaffer
Megan
M
Pineda
Gina
G
Sinha
Siddhartha K
SK
Schneida
Elaine
E
Nasir
Huma
H
Shewale
Jaiprakash G
JG
D041322:Chromosomes, Human, Y; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting / Q000379:methods*; D030541:Databases, Genetic; D005260:Female; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D044469:Racial Groups / Q000235:genetics*; D012016:Reference Values; D020080:Tandem Repeat Sequences; D014481:United States
D004247:DNA