Towards a multi-site synthetic vaccine to foot-and-mouth disease: addition of discontinuous site peptide mimic increases the neutralization response in immunized animals.
Synthetic replicas of both antigenic sites A and D of foot-and-mouth disease virus have been tested as a first step towards a multicomponent peptide vaccine candidate. A first evaluation has been performed by neutralization assays on cells with serum mixtures from guinea pigs immunized independently with site A (A24) and site D (D8) peptides. The addition of site D antibodies to site A antibodies has a synergistic effect on neutralization. In a second group of experiments, guinea pigs have been immunized with a dendrimeric tetravalent (MAP) presentation of site A peptide, alone or in combination with D8, using the same total peptide dose. While the first inoculation gives a preferential response to site A-only antigen, specific response to site D and global neutralization levels significantly increase after reimmunization, reflecting a synergistic effect of site D.
Department of Experimental and Health Sciences, Pompeu Fabra University, Dr. Aiguader 80, 08003 Barcelona, Spain.
Judit
J
Villén
Eliandre
E
de Oliveira
José Ignacio
JI
Núñez
Nicolás
N
Molina
Francisco
F
Sobrino
David
D
Andreu
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D006168:Guinea Pigs; D007114:Immunization; D008297:Male; D008958:Models, Molecular; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011487:Protein Conformation; D022223:Vaccines, Subunit / Q000627:therapeutic use; D014765:Viral Vaccines / Q000627:therapeutic use*
D022223:Vaccines, Subunit; D014765:Viral Vaccines