Serological detection of type-specific IgG to herpes simplex virus by novel ELISAs based on recombinant and highly purified glycoprotein G.
Most commercially available tests for detection of herpes simplex virus-specific antibodies are not able to differentiate reliably between antibodies to the viral types 1 and 2. Therefore, the objective of the present study was to evaluate two novel enzyme-linked immunosorbent assays on the basis of recombinant and highly purified glycoprotein G for determination of herpes simplex virus type-specific antibodies. Procedures approved by the US Food and Drug Administration served as reference. 100% sensitivity was calculated for both the type 1 and the type 2 immunoassay. For the type 1 immunoassay, the specificity was 98.6% and the type 2 immunoassay had a specificity of 99.3%. There was a very good correlation between the results of the reference procedures and the evaluated tests. The novel immunoassays can be recommended for the reliable type-specific diagnosis of latent herpes simplex virus infections.
Institute of Virology and Antiviral Therapy, Friedrich-Schiller University, Jena, Germany. Andreas.Sauerbrei@med.uni-jena.de
Andreas
A
Sauerbrei
Peter
P
Wutzler
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D013045:Species Specificity; D014759:Viral Envelope Proteins
D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C050591:glycoprotein gG-1, herpes simplex virus type 1