[Polymorphism of STR loci D12S391/D18S865 in Wenzhou Han population].
OBJECTIVE
To investigate the polymorphism and forensic efficiency values of STR loci D12S391/D18S865


METHODS
Population studies on D12S391 and D18S865 were carried out in a sample of 454 unrelated Wenzhou Han individuals using PCR followed by PAGE and silver stain detection.


RESULTS
11/7 alleles and 50/21 genotypes of D12S391/D18S865 were respectively observed among the 454 unrelated individuals. The genotype frequency matched the Hardy-Weinberg equilibrium, The heterozygotes (H) in D12S391/D18S865 were 0.7974/0.7379, Power of Exclusion were 0.9566/0.9077, polymorphism information content (PIC) were 0.8260/0.7279 respectively.


CONCLUSIONS
D12S391 and D18S865 were high polymorphic loci and the frequencies were in accordance with Hardy-Weinberg equilibrium (P>0.5), so the two loci can be used in forensic medicine and in other genetic research areas.
The Molecular Biology Research Center of Wenzhou Medical College, WenZhou 325027, China.
Shu-zhen
SZ
Wu
Hong-qin
HQ
Zhang
Yun-tian
YT
Bi
D000483:Alleles; D002681:China / Q000208:ethnology; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005554:Forensic Medicine; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D004247:DNA