Thidiazuron-induced morphogenetic response in petiole cultures of Pelargonium x hortorum and Pelargonium x domesticum and its histological analysis.
The possibility of inducing somatic embryogenesis in petiole cultures of two cultivars of Pelargonium x hortorum and of one cultivar of Pelargonium x domesticum using thidiazuron (TDZ) was investigated. Petioles were cultivated on a modified Murashige and Skoog medium with different concentrations and application periods of TDZ. Regeneration was achieved with all TDZ treatments for all cultivars and was highly variable. Shoots of different shapes and somatic embryo-like structures were observed. Histological examination revealed that no somatic embryos were formed, and regenerants had to be classified as shoots and shoot-like or leaf-like structures. The importance of these results on the classification of regeneration induced by TDZ in these species and on the propagation of these pelargoniums is discussed.
Department Plant Propagation, Institute of Vegetable and Ornamental Crops Grossbeeren/Erfurt eV, Kühnhäuser Str. 101, 99189, Erfurt-Kühnhausen, Germany. haensch@erfurt.igzev.de
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D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology; D038081:Organogenesis / Q000187:drug effects / Q000502:physiology; D031316:Pelargonium / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D010671:Phenylurea Compounds / Q000494:pharmacology*; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D012639:Seeds / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D013830:Thiadiazoles / Q000494:pharmacology*; D046509:Tissue Culture Techniques
D006133:Growth Substances; D010671:Phenylurea Compounds; D013830:Thiadiazoles; C016785:thidiazuron