Inhibitory mechanism of inteferon-gamma on human fibroblasts from Tenon's capsule.
The inhibitory mechanism of interferon-gamma (IFN-gamma) on the fibroblasts from Tenon's capsule was studied. By using immunohistochemical SP method and pathological image system, the inhibitory effects of IFN-gamma on the expression of transforming growth factor beta receptor I in the in vitro cultured fibroblasts from Tenon's capsule were quantitatively analyzed. The results showed that IFN-gamma could reduce the expression of transforming growth factor beta receptor I in the fibroblasts with the following dose-effect relationship: Y = 1937.5-134.2 Igx (r=-0.971, P<0.01). It was concluded that IFN-gamma could inhibit the expression of transforming growth factor beta receptor I in the fibroblasts from Tenon's capsule. The modulation of the transforming growth factor beta receptor I expression by IFN-gamma may be beneficial to the alleviation of the hyperplasia of scar after trabeculectomy.
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 43002, China.
Bo
B
Han
Yizhen
Y
Hu
Xinchun
X
Xiong
D003228:Conjunctiva / Q000378:metabolism / Q000473:pathology*; D003238:Connective Tissue / Q000187:drug effects*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D018463:Filtering Surgery; D005901:Glaucoma / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis
D018125:Receptors, Transforming Growth Factor beta; D007371:Interferon-gamma