Interactions and chaperone function of alphaA-crystallin with T5P gammaC-crystallin mutant.
T5P gammaC-crystallin mutation is associated with Coppock-like cataract, one of the autosomal dominant congenital cataracts. It is not known why the abundant alpha-crystallin cannot prevent the mutation-related aggregation. Our previous studies indicate that the mutation changes conformation and reduces solubility and stability, but it is not known whether it is these events or the loss of interaction with other crystallins that causes the cataract. It is also not known whether the alpha-crystallin can protect T5P mutant as effectively from heat-induced aggregation as the wild-type (WT) gammaC-crystallin. To investigate the mechanism of interactions and chaperone function between alphaA- and gammaC-crystallin, human alphaA-crystallin and W9F mutant as well as WT gammaC-crystallin and T5P mutant were cloned. Interactions between alphaA- and gammaC-crystallin were studied with fluorescence resonance energy transfer (FRET), and chaperone activity was assessed by the suppression of heat-induced aggregation of substrate proteins. Conformational changes of substrate proteins were studied by spectroscopic measurements. The results indicate that the T5P mutant showed a slightly greater FRET than WT gammaC-crystallin with alphaA-crystallin, and alphaA-crystallin could effectively prevent both WT and T5P gammaC-crystallin from heat-induced aggregation. Spectroscopic measurements show that both alphaA-crystallin and gammaC-crystallin underwent only slight conformational change after chaperone binding. Together with previous results obtained with a two-hybrid system assay of interactions between alphaA- and gammaC-crystallin, the present FRET and chaperone results indicate that loss of interactions of T5P mutant with other crystallins may play a larger role than the protection afforded by chaperone-like activity in Coppock-like cataract.
Center for Ophthalmic Research, Brigham and Women's Hospital, 221 Longwood Avenue, Boston, MA 02115, USA. jliang@rics.bwh.harvard.edu
Jack J-N
JJ
Liang
D002386:Cataract / Q000378:metabolism / Q000503:physiopathology; D031541:Fluorescence Resonance Energy Transfer; D006358:Hot Temperature; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D009154:Mutation; D011487:Protein Conformation; D017510:Protein Folding; D025941:Protein Interaction Mapping; D038202:alpha-Crystallin A Chain / Q000378:metabolism*; D038222:gamma-Crystallins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D018832:Molecular Chaperones; D038202:alpha-Crystallin A Chain; D038222:gamma-Crystallins