[Influence of calcium ionophore A23187 on neurotransmitter release in rat brain synaptosomes].
The effect of calcium ionophore A23187 on the release of nonmetabolizable glutamate analogues [3H]D-aspartate and the exocytosis registered by fluorescent dyes in synaptosomes was investigated. It was shown that A23187 is able to induce neurotransmitter release both in calcium-containing and calcium-free medium, the effect in the latter case being more pronounced. Calcium ionophore is able to induce exocytosis registered by acridine orange and FM 2-10. The influence of A23187 on the fluorescence of acridine orange was mainly calcium-independent, whereas the change in the fluorescence of FM 2-10 was calcium-dependent. It was suggested that the calcium-independent increase in acridine orange fluorescence is related to the dissipation of pH gradient in synaptic vesicles. Probably, the calcium-independent release of D-aspartate is also associated with the dissipation of pH gradient and subsequent leakage of neurotransmitters.
Vasim
T V
TV
Lavrukevich
T V
TV
Rakovich
A A
AA
Fedorovich
S V
SV
Konev
S V
SV
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology*; D005089:Exocytosis; D005453:Fluorescence; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D000001:Calcimycin