Molecular analysis of Plasmodium ovale variants.
Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data. Three different SSUrRNA sequences (Pov 1-3) were found in each variant-type isolate, and two different SSUrRNA sequences (Poc 1-2) in each classic-type isolate. Pov 1-3 were closer to sequences previously found in the Cameroon and MAL/MAI isolates, whereas Poc 1-2 were closer to sequences previously found in two clones of the Nigerian I/CDC strain. The 3' half of Pov 1-3 was identical to the partial sequence of the SSUrRNA gene from the London School (LS) strain. Results support grouping P. ovale into two groups, the classic type (including the Nigerian I/CDC strain) and the variant type (Cameroon, MAL/MAI, and LS isolates).
Nagoya University Graduate School of Medicine, Japan.
Thin Thida
TT
Win
Amadu
A
Jalloh
Indah Setyawati
IS
Tantular
Takafumi
T
Tsuboi
Marcelo Urbano
MU
Ferreira
Masatsugu
M
Kimura
Fumihiko
F
Kawamoto
D000818:Animals; D001210:Asia, Southeastern; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008288:Malaria / Q000469:parasitology; D008969:Molecular Sequence Data; D041122:Plasmodium ovale / Q000145:classification* / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
D015800:Protozoan Proteins; D012335:RNA, Ribosomal; D003546:Cysteine Endopeptidases