A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining.
We report on the development of a detailed two-dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time-of-flight (TOF/TOF)-mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two-dimensional gel electrophoresis (2-DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein-specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations.
Hayduk
Eric J
EJ
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA. KHL9@cornell.edu
Choe
Leila H
LH
Lee
Kelvin H
KH
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes / Q000737:chemistry*; D013058:Mass Spectrometry; D010750:Phosphoproteins / Q000378:metabolism*; D020543:Proteome
D005456:Fluorescent Dyes; D010750:Phosphoproteins; D020543:Proteome