High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples.
A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively.
Computational Analytical and Structural Sciences, Psychiatry CEDD, GlaxoSmithKline, Via A. Fleming 4, 37135 Verona, Italy. mark.e.hows@gsk.com
Mark E P
ME
Hows
Laurent
L
Lacroix
Christian
C
Heidbreder
Andrew J
AJ
Organ
Ajit J
AJ
Shah
D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D001923:Brain Chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003042:Cocaine / Q000032:analysis* / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017551:Microdialysis / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015306:Biogenic Monoamines; D003042:Cocaine