[Effect of borneol on the intercellular tight junction and pinocytosis vesicles in vitro blood-brain barrier model].
OBJECTIVE
To explore the mechanism of borneol in opening the blood-brain barrier (BBB).


METHODS
Borneol contained serum was prepared and using Matin-Darby canine kidney epithelium (MDCKE) cell line as the in vitro BBB model to observe the effects of borneol on intercellular tight junction (ICTJ) and pinocytosis vesicles of BBB model.


RESULTS
Borneol reduced the ICTJ and caused increase of the number and enlarged the diameter of vesicles. The ICTJ was opened firstly 4 hrs after borneol treatment, then the pinocytosis was affected 24 hrs later. The effects disappeared 24 hrs after removal of the borneol contained serum, indicating that the above-mentioned effects were reversible.


CONCLUSIONS
Borneol could obviously loosen the ICTJ in BBB, accelerate the transportation of substance through the intercellular passage, it also could increase the number and volume of pinocytosis vesicles in BBB cells, thus to accelerate the transportation of substance by way of cell pinocytosis.
Institute of Clinical Pharmacology, Guangzhou TCM University, Guangzhou 510405. mileschen6@163.com
Yan-ming
YM
Chen
Ning-sheng
NS
Wang
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000502:physiology; D001892:Camphanes / Q000494:pharmacology*; D002460:Cell Line; D002463:Cell Membrane Permeability; D004847:Epithelial Cells / Q000166:cytology; D007668:Kidney / Q000166:cytology; D008297:Male; D008959:Models, Neurological; D010873:Pinocytosis / Q000187:drug effects*; D011817:Rabbits; D019108:Tight Junctions / Q000187:drug effects*
D001892:Camphanes; C022871:isoborneol