Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C.
A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. The human Aurora paralogues Aurora-A, Aurora-B, and Aurora-C have a highly conserved catalytic domain. Extensive studies on the role of Aurora-A and Aurora-B have revealed distinct localizations and functions in regulating mitotic processes, whereas little is known about Aurora-C. The present study shows that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. We show that INCENP binds and activates Aurora-C in vivo and in vitro. Furthermore, Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells. We therefore suggest that Aurora-C is a novel chromosomal passenger protein that cooperates with Aurora-B to regulate mitotic chromosome dynamics in mammalian cells.
Li
Xiangyu
X
Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550. turano@med.nagoya-u.ac.jp
Sakashita
Gyosuke
G
Matsuzaki
Hideki
H
Sugimoto
Kenji
K
Kimura
Keiji
K
Hanaoka
Fumio
F
Taniguchi
Hisaaki
H
Furukawa
Koichi
K
Urano
Takeshi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D019556:COS Cells; D020134:Catalytic Domain; D002453:Cell Cycle; D002460:Cell Line; D002503:Centromere / Q000648:ultrastructure*; D002843:Chromatin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism; D002875:Chromosomes / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry; D006801:Humans; D047468:Immunoprecipitation; D007399:Interphase; D018613:Microscopy, Confocal; D008938:Mitosis; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013347:Subcellular Fractions; D013997:Time Factors; D014162:Transfection
D000911:Antibodies, Monoclonal; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; C083767:INCENP protein, human; C576921:AURKB protein, human; C576927:AURKC protein, human; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases