[The study on expression of NF-kappaB in experimental brain contusion in rats].
OBJECTIVE
To investigate the expression of NF-kappaB in different post-traumatic intervals and severity of brain injury.


METHODS
Fifty-four brain tissue samples of slight (n=24), moderate (n=24) brain injury, sham (n=3) and control (n=3) of rat were examined by immunohistochemical staining, westernblot and RT-PCR.


RESULTS
Up-regulating of NF-kappaB expression was found in tissues from traumatic brain injury compared with controls in early 1 hour after TBI, and reached peak at 24h and 48h and disappeared after 7 days. The expression of NF-kappaB mRNA has association with contusion severity.


CONCLUSIONS
NF-kappaB may benefit to the estimation of posttraumatic intervals of brain injury.
Tao
Lu-yang
LY
School of Medicine, Suzhou University, Suzhou 215007, China. luyangtao@suda.edu.cn
Chen
Xi-ping
XP
Ding
Mei
M
D000818:Animals; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D015601:Injury Severity Score; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D013997:Time Factors
D016328:NF-kappa B; D012333:RNA, Messenger