Tackling the plant proteome: practical approaches, hurdles and experimental tools.
The study of complex biological questions through comparative proteomics is becoming increasingly attractive to plant biologists as the rapidly expanding plant genomic and expressed sequence tag databases provide improved opportunities for protein identification. This review focuses on practical issues associated with comparative proteomic analysis, including the challenges of effective protein extraction and separation from plant tissues, the pros and cons of two-dimensional gel-based analysis and the problems of identifying proteins from species that are not recognized models for functional genomic studies. Specific points are illustrated using data from an ongoing study of the tomato and pepper fruit proteomes.
Rose
Jocelyn K C
JK
Department of Plant Biology, Cornell University, Ithaca, NY 147853, USA. jr286@cornell.edu
Bashir
Sajid
S
Giovannoni
James J
JJ
Jahn
Molly M
MM
Saravanan
Ramu Subramanian
RS
D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D020543:Proteome
D010940:Plant Proteins; D020543:Proteome