Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei.
A food-grade strain with nisRK stably integrated into the genome, was constructed in order to implement the nisin-controlled expression system (NICE) in Lactobacillus casei ATCC393. Expression of beta-glucuronidase (gus) reporter gene was employed to optimize the system, which has been successfully used to produce the main antigenic protein from Norwalk virus, opening new perspectives for producing edible vaccines.
Martín
M Cruz
MC
Instituto de Productos Lácteos de Asturias (CSIC), 33300 Villaviciosa, Asturias, Spain.
Fernández
María
M
Martin-Alonso
José M
JM
Parra
Francisco
F
Boga
José A
JA
Alvarez
Miguel A
MA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics*; D005516:Food Microbiology; D005786:Gene Expression Regulation; D000071677:Histidine Kinase; D007780:Lacticaseibacillus casei / Q000235:genetics*; D009561:Nisin / Q000494:pharmacology*; D009663:Norwalk virus / Q000276:immunology*; D011494:Protein Kinases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D000900:Anti-Bacterial Agents; D000956:Antigens, Viral; D001426:Bacterial Proteins; D036022:Capsid Proteins; D014157:Transcription Factors; C080935:nisR protein, Lactococcus lactis; D009561:Nisin; D011494:Protein Kinases; D000071677:Histidine Kinase