Recombinant fraction 1 protein of Yersinia pestis activates murine peritoneal macrophages in vitro.
Fraction 1 antigen of Yersinia pestis is a capsule protein of 17.5kDa, known to induce thymocyte proliferation and have anti-phagocytic role in macrophages. It serves as a major protective antigen against challenge of Y. pestis by inducing high concentration of IgG1 antibody response. In the present investigation it is observed that 10microg/ml of rF1 antigen activated murine peritoneal macrophages in vitro. rF1 induced the production of TNF-alpha, IL-1, IL-6, and NO. rF1 treatment also induced increased transcription of IFN-gamma and its related chemokines KC, IP-10, MIP-1alpha, MIP-1beta, MCP-1, RANTES in macrophages. Significantly increased transcription of TLR5 was observed in macrophages treated with rF1, while the expression of TLR2 and TLR4 remained unaffected.
School of Biotechnology, Banaras Hindu University, Varanasi 221 005, UP, India. ajit.sodhi@lycos.com
Ajit
A
Sodhi
Rajesh Kumar
RK
Sharma
H V
HV
Batra
Urmil
U
Tuteja
D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism; D008262:Macrophage Activation / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015010:Yersinia pestis / Q000378:metabolism*
D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D012273:Ribulose-Bisphosphate Carboxylase