Lack of hydroxyl radical generation upon central administration of methamphetamine in rat caudate nucleus: a microdialysis study.
The most widely accepted concept of oxidative damage centers on the formation of hydroxyl radical (*OH) which has an extremely short-life and is the major damaging free radical. It was suggested that methamphetamine (METH) toxicity is mediated via production of *OH, as measured by 2,3-dihydroxybenzoic acid (2,3-DHBA). In this study we compared the effects of local caudate nucleus perfusion of METH with systemic administration of METH on *OH generation in relation to DA release. Local perfusion of METH (5 mM, 140 min) induced a higher level of dopamine (DA) release compared to the first METH injection (10 mg/kg, 3 times, i.p.). No significant correlation was found between changes in extracellular DA levels and *OH generation when perfusing METH locally; however, both increased after systemic METH administration.
Neurochemistry Laboratory, Division of Neurotoxicology, HFT-132, NCTR/FDA, Jefferson, AR 72079, USA.
Frederico C
FC
Pereira
Tice R
TR
Macedo
Syed Z
SZ
Imam
Carlos F
CF
Ribeiro
Syed F
SF
Ali
D000818:Animals; D002421:Caudate Nucleus / Q000187:drug effects* / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage*; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017665:Hydroxyl Radical; D008694:Methamphetamine