Single molecule acid-base kinetics and thermodynamics.
We report a method in which temperature dependent single-molecule fluorescence measurements are used to study the kinetics and thermodynamics of the acid-base interaction in films of photoresist polymer. We use the two distinct fluorescent prototropic forms of Coumarin 6 (C6-->C6+) to indicate the state of the acid-base system. Data are analyzed using a statistical model of the intensity probability distributions, yielding temperature dependent proton exchange rates, which is confirmed through agreement with a simple two-state Monte Carlo model. The temperature dependent rates are used to calculate the activation enthalpy for proton exchange.
Mason
Michael D
MD
Department of Applied Physics, Yale University, New Haven, Connecticut 06520, USA.
Ray
Krishanu
K
Grober
Robert D
RD
Pohlers
Gerd
G
Cameron
James
J
D000143:Acids; D003374:Coumarins / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D015233:Models, Statistical; D009010:Monte Carlo Method; D017785:Photons; D011522:Protons; D013696:Temperature; D013816:Thermodynamics
D000143:Acids; D003374:Coumarins; D011522:Protons; C030123:coumarin