The relationship among serum cytokines, chemokine, nitric oxide, and leptin in children with type 1 diabetes mellitus.
OBJECTIVE
The aim of this study was to investigate the relationship between cytokines, leptin and vascular tone-related chemokine and nitric oxide (NO) in type 1 diabetic children.


METHODS
Serum samples were collected from 58 children with type 1 diabetes and 33 of their healthy siblings.


RESULTS
Serum interleukin (IL)-8 was significantly higher and serum nitric oxide was significantly lower in the children with diabetes than in their healthy siblings. Stepwise regression analysis showed that there were significantly positive correlations between IL-1beta and IL-6, IL-1beta and nitric oxide, IL-4 and tumor-necrosis factor (TNF)-alpha, IL-4 and leptin, IL-8 and IL-2, and interferon (IFN)-gamma and IL-6, as well as significantly inversed correlations between IL-6 and IL-2, IL-8 and interferon-gamma, and leptin and TNF-alpha in siblings, not in the children with diabetes. However, there were significantly positive correlations between IL-2 and IL-4, IL-2 and leptin, IL-4 and IL-6, and TNF-alpha and IL-6 in children with diabetes.


CONCLUSIONS
Our results suggest that the alterations of circulating IL-8 and nitric oxide levels and cytokine network in children with diabetes may be associated with the cardiovascular disease in their adulthood.
Lo
Hui-Chen
HC
Department of Bioscience Technology, Chang-Jung Christian University, Tainan 711, Taiwan.
Lin
Su-Chen
SC
Wang
Yu-Mei
YM
D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D001835:Body Weight; D018925:Chemokines / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood / Q000378:metabolism; D020738:Leptin / Q000097:blood*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D012044:Regression Analysis
D018925:Chemokines; D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8; D020738:Leptin; D009569:Nitric Oxide; D007371:Interferon-gamma