Overexpression of c-Jun induced by quercetin and resverol inhibits the expression and function of the androgen receptor in human prostate cancer cells.
Previously, we reported that quercetin and resveratrol inhibit the function of androgen receptor (AR). Further studies showed that these two polyphenols caused an increase in expression of c-Jun as well as its phosphorylated form in a dose-dependent manner in prostatic cell lines. Gel shift assay showed that induced c-Jun has specific DNA binding activity. Transient transfections demonstrated that c-Jun repressed prostate-specific antigen promoter activity and transcriptional activity of the AR promoter. These results support a mechanism in which overexpressed c-Jun mediates inhibitory effect on the function of AR. These polyphenols might potentially be useful in prostate cancer prevention.
Department of Urology, Mayo Clinic college of Medicine, Mayo Clinic, Guggenheim Building 520B, Rochester, MN 55905, USA.
Huiqing
H
Yuan
Yunqian
Y
Pan
Charles Y F
CY
Young
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010636:Phenols; D011471:Prostatic Neoplasms / Q000473:pathology*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D011794:Quercetin / Q000494:pharmacology*; D011944:Receptors, Androgen / Q000187:drug effects* / Q000502:physiology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols; D016755:Proto-Oncogene Proteins c-jun; D011944:Receptors, Androgen; D013267:Stilbenes; D011794:Quercetin; D000077185:Resveratrol