Dynamic alterations of specific histone modifications during early murine development.
In order to investigate whether covalent histone modifications may be involved in early embryonic reprogramming events, changes in global levels of a series of histone tail modifications were studied during oocyte maturation and pre-implantation mouse development using indirect immunofluorescence and scanning confocal microscopy. Results showed that histone modifications could be classified into two strikingly distinct categories. The first contains stable 'epigenetic' marks such as histone H3 lysine 9 methylation [Me(Lys9)H3], histone H3 lysine 4 methylation [Me(Lys4)H3] and histone H4/H2A serine 1 phosphorylation [Ph(Ser1)H4/H2A]. The second group contains dynamic and reversible marks and includes hyperacetylated histone H4, histone H3 arginine 17 methylation [Me(Arg17)H3] and histone H4 arginine 3 methylation [Me(Arg3)H4]). Our results also showed that removal of these marks in eggs and early embryos occurs during metaphase suggesting that the enzymes responsible for the loss of these modifications are probably cytoplasmic in nature. Finally, we provide data demonstrating that treatment of cellular histones with peptidylarginine deiminase (PAD) results in loss of staining for the histone H4 arginine 3 methyl mark, suggesting that PADs can reverse histone arginine methyl modifications.
Department of Cell Biology, University of Virginia Health Science Center, PO Box 800732, Charlottesville, VA 22908, USA.
Olga F
OF
Sarmento
Laura C
LC
Digilio
Yanming
Y
Wang
Julie
J
Perlin
John C
JC
Herr
C David
CD
Allis
Scott A
SA
Coonrod
D000107:Acetylation; D000818:Animals; D001120:Arginine / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006657:Histones / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008677:Metaphase / Q000502:physiology*; D008745:Methylation; D051379:Mice; D018613:Microscopy, Confocal; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D010766:Phosphorylation; D000076342:Protein-Arginine Deiminases; D012694:Serine / Q000378:metabolism
D006657:Histones; D012694:Serine; D001120:Arginine; D006867:Hydrolases; D000076342:Protein-Arginine Deiminases; D008239:Lysine