[Structural analysis of 22 full-length hepatitis B virus genomes isolated from patients with hepatocellular carcinoma].
OBJECTIVE
To study the characteristics of full-length Hepatitis B Virus (HBV) genomes isolated from patients with hepatocellular carcinoma (HCC).


METHODS
The full-length HBV genomes from the serum of HBsAg positive HCC patients were amplified by PCR, and then sequenced and analyzed its structure.


RESULTS
Twenty-two full-length HBV DNAs were obtained from different patients of HCC. Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C and serotype adr or adw2. Structural analysis showed that HBV obtained shared meaningful consensus mutations in B/T cell epitopes of surface and core antigens, transactivating domain of X protein and enhancer II/core promoter regions as compared to standard strains.


CONCLUSIONS
Genotype and gene mutation of HBV may be closely correlated with the carcinogenesis of HBV-related hepatocellular carcinoma.
Lin
Xu
X
Research Center of Molecular Medicine, Fujian Medical University, Fuzhou 350004, China. Linxu70@hotmail.com
Xu
Xiao
X
Zheng
Da-li
DL
Lin
Jian-yin
JY
D000596:Amino Acids / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006512:Hepatitis B Core Antigens / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D009154:Mutation; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
D000596:Amino Acids; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein