Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats.
OBJECTIVE
Microcirculatory disturbances are important early pathophysiological events in various organs during acute pancreatitis. The aim of the study was to evaluate changes in microperfusion of the pancreas, liver, kidney, stomach, colon, skeletal muscle, and to investigate the influence of heparin on the organ microcirculation in caerulein-induced experimental acute pancreatitis.


METHODS
Acute pancreatitis was induced by 4 intraperitoneal injections of caerulein (Cn) (15 microg/kg). The organ microcirculation was measured by laser Doppler flowmetry. Serum interleukin 6 and hematocrit levels were analysed.


RESULTS
Acute pancreatitis resulted in a significant drop of microperfusion in all examined organs. Heparin administration (2 x 2.5 mg/kg) improved the microcirculation in pancreas (36.9 +/- 4% vs 75.9 +/- 10%), liver (56.6 +/- 6% vs 75.2 +/- 16%), kidney (45.1 +/- 6% vs 79.3 +/- 5%), stomach (65.2 +/- 8% vs 78.1 +/- 19%), colon (69.8 +/- 6% vs 102.5 +/- 19%), and skeletal muscle (59.2 +/- 6% vs 77.9 +/- 13%). Heparin treatment lowered IL-6 (359.0 +/- 66 U/mL vs 288.5 +/- 58 U/mL) and hematocrit level (53 +/- 4% vs 46 +/- 3%).


CONCLUSIONS
Heparin administration has a positive influence on organ microcirculatory disturbances accompanying experimental Cn-induced acute pancreatitis.
Department of General and Gastroenterological Surgery, St. Vincent a'Paulo Hospital, Gdynia, Poland. marek-dobosz@wp.pl
Marek
M
Dobosz
Lucjanna
L
Mionskowska
Stanislaw
S
Hac
Sebastian
S
Dobrowolski
Dariusz
D
Dymecki
Zdzislaw
Z
Wajda
D000208:Acute Disease; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002108:Ceruletide; D006400:Hematocrit; D006493:Heparin / Q000494:pharmacology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000187:drug effects
D000925:Anticoagulants; D002108:Ceruletide; D006493:Heparin