Angiogenic and cardiac functional effects of dual gene transfer of VEGF-A165 and PDGF-BB after myocardial infarction.
Therapeutic angiogenesis is a potential treatment modality for myocardial ischemia. phVEGF-A(165), phPDGF-BB, or a combination of the two were injected into the myocardial infarct border zone in rats 7 days after ligation of the coronary left anterior descending artery. Cardiac function was measured by echocardiography. Hearts were harvested 1 and 4 weeks after plasmid injection. phVEGF-A(165) increased capillary density more than phPDGF-BB, and phPDGF-BB preferentially stimulated arteriolar growth. The combination increased both capillaries and arterioles but did not enhance angiogenesis any more than single plasmid treatments did. VEGF-A(165) and the combination of phVEGF-A(165) and phPDGF-BB counteracted left ventricular dilatation after 1 week but did not counteract further deterioration.
Department of Cardiology, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden.
Xiaojin
X
Hao
Agneta
A
Månsson-Broberg
Pontus
P
Blomberg
Göran
G
Dellgren
Anwar J
AJ
Siddiqui
Karl-Henrik
KH
Grinnemo
Eva
E
Wärdell
Christer
C
Sylvén
D000818:Animals; D000077214:Becaplermin; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D018919:Neovascularization, Physiologic; D010957:Plasmids / Q000008:administration & dosage; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D000077214:Becaplermin