Genetics of spinosad resistance in a multi-resistant field-selected population of Plutella xylostella.
Resistance to the bacteria-derived insecticides spinosad (Conserve), abamectin (Vertimec), Bacillus thuringiensis var kurstaki (Btk) (Dipel), B thuringiensis var aizawai (Bta) (Xentari), B thuringiensis crystal endotoxins Cry1Ac and Cry1Ca, and to the synthetic insecticide fipronil was estimated in a freshly-collected field population (CH1 strain) of Plutella xylostella (L) from the Cameron Highlands, Malaysia. Laboratory bioassays at G1 indicated significant levels of resistance to spinosad, abamectin, Cry1Ac, Btk, Cry1Ca, fipronil and Bta when compared with a laboratory insecticide-susceptible population. Logit regression analysis of F1 reciprocal crosses indicated that resistance to spinosad in the CH1 population was inherited as a co-dominant trait. At the highest dose of spinosad tested, resistance was close to completely recessive, while at the lowest dose it was incompletely dominant. A direct test of monogenic inheritance based on a back-cross of F1 progeny with CH1 suggested that resistance to spinosad was controlled by a single locus.
Sayyed
Ali H
AH
Department of Biological Sciences, Imperial College London, Silwood Park campus, Ascot, Berkshire, UK. h.sayyed@imperial.ac.uk
Omar
Dzolkhifli
D
Wright
Denis J
DJ
D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity; D001427:Bacterial Toxins / Q000633:toxicity; D004338:Drug Combinations; D004731:Endotoxins / Q000633:toxicity; D005799:Genes, Dominant / Q000235:genetics; D005808:Genes, Recessive / Q000235:genetics; D005816:Genetic Complementation Test; D006460:Hemolysin Proteins; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000633:toxicity*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000633:toxicity; D018942:Macrolides / Q000633:toxicity*; D009036:Moths / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011498:Protein Precursors / Q000633:toxicity; D011720:Pyrazoles / Q000633:toxicity
D000083722:Bacillus thuringiensis Toxins; C029103:Bacillus thuringiensis protoxin; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004338:Drug Combinations; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D018942:Macrolides; D011498:Protein Precursors; D011720:Pyrazoles; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C048324:abamectin; D007559:Ivermectin; C082360:fipronil; C415329:spinosad