A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin.
A hemorrhagic proteinase, jerdohagin, was purified from Trimeresurus jerdonii venom by gel filtration and ion-exchange chromatographies. It was a single chain polypeptide with an apparent molecular weight of 96 kDa as estimated by SDS-PAGE under the non-reducing and reducing conditions. Internal peptide sequencing indicated that it consisted of metalloproteinase, disintegrin-like and cysteine-rich domains and belonged to the class III snake venom metalloproteinases (class P-III SVMPs). Like other typical metalloproteinases, hemorrhagic activities of jerdohagin were completely inhibited by EDTA, but not by PMSF. Jerdohagin preferentially degraded alpha-chain of human fibrinogen. Interestingly, jerdohagin did not activate human prothrombin, whereas it cleaved human prothrombin and fragment F1 of activated human prothrombin.
Chen
Run-Qiang
RQ
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China.
Jin
Yang
Y
Wu
Jian-Bo
JB
Zhou
Xing-Ding
XD
Li
Dong-sheng
DS
Lu
Qiu-Min
QM
Wang
Wan-Yu
WY
Xiong
Yu-Liang
YL
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000378:metabolism*; D004492:Edetic Acid / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D006470:Hemorrhage / Q000139:chemically induced; D006801:Humans; D007328:Insulin / Q000378:metabolism; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011516:Prothrombin / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017841:Trimeresurus
D003435:Crotalid Venoms; D007328:Insulin; D010446:Peptide Fragments; C045879:prothrombin fragment 1.2; D011516:Prothrombin; D005340:Fibrinogen; D004492:Edetic Acid; D045726:Metalloproteases