From IHF protein to design and synthesis of a sequence-specific DNA bending peptide.
The design and synthesis of a small peptide that mimics the integration host factor (IHF), a major nucleoid-associated protein, is reported. IHF induces DNA compaction by sequence-specific binding that leads to significant bending of the DNA double strand. In a modular approach a small L-lysine dendrimer responsible for nonspecific charge-charge interactions was linked to a cyclopeptide. The latter was designed for specific DNA recognition in the minor groove followed by bending of the double strand.
Liebler
Eduard K
EK
Institut für Organische und Biomolekulare Chemie, Universität Göttingen, Tammannstrasse 2, D-37077 Göttingen, Germany.
Diederichsen
Ulf
U
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D034982:Integration Host Factors / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation
D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D010455:Peptides; D010456:Peptides, Cyclic; D004247:DNA