Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli.
Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-alpha-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.
Department of Biology, National Cheng Kung University, Tainan City, Taiwan ROC.
Shun-Fen
SF
Tzeng
May J
MJ
Tsai
Shih-Chieh
SC
Hung
Henrich
H
Cheng
D015153:Blotting, Western / Q000379:methods; D001853:Bone Marrow / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003563:Cyclic CMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005347:Fibroblasts / Q000502:physiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007381:Intermediate Filament Proteins; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D009479:Neuropeptides / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D016704:Synapsins / Q000378:metabolism; D015534:Trans-Activators / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
C495401:ADCYAP1 protein, human; D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D007381:Intermediate Filament Proteins; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D016704:Synapsins; D015534:Trans-Activators; D014404:Tubulin; C046643:alpha-internexin; C044882:neurofilament protein L; D003563:Cyclic CMP; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate