Effect of EGCG, a major component of green tea, on the expression of Ets-1, c-Fos, and c-Jun during angiogenesis in vitro.
In this study, we used rat aortic endothelial cells and human umbilical vein endothelial cells growing in collagen gel as a model system to study the tea catechin, (-)-epigallocatechin (EGCG), on the differential expression of transcription factors, Ets-1, c-Fos, and c-Jun during endothelial morphogenesis in vitro. Cells growing in collagen gel from 0 to 2 h remained spherical. After 6 h, the cells became elongated and underwent morphogenesis. At 24 h, cells started to organize to form capillary-like tubular structures. At 48 h, most cells in the gel formed a network of branching and tubular structures. Immunohistochemistry and immunofluorescence microscopy showed that the reaction products of Ets-1, c-Fos, and c-Jun presented predominantly in the nucleus. No reaction products appeared in the cells that were organized to form capillary-like tubular structures. After adding EGCG to the collagen gel, cells became elongated in the first 6 h and then remained quiescent. No tubular structure was formed. Western blotting showed that the levels of Ets-1, c-Fos, and c-Jun reached the highest levels at 12-24 h, decreasing to the basal level at 48 h. After adding EGCG to the collagen gel, levels were lower than for the non-EGCG-treated groups. These results indicated that the morphogenesis of endothelial cells in collagen gel was inhibited by EGCG through the down-regulation of Ets-1, c-Fos, and c-Jun.
Taichung Veterans General Hospital, Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
Hui Chin
HC
Lai
Wei Ting
WT
Chao
Ying Tsung
YT
Chen
Vivian Cheng
VC
Yang
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology; D015153:Blotting, Western; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002455:Cell Division; D003094:Collagen; D003470:Culture Media; D015536:Down-Regulation; D042783:Endothelial Cells; D009024:Morphogenesis; D009389:Neovascularization, Pathologic; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D050783:Proto-Oncogene Proteins c-ets; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D051381:Rats; D014157:Transcription Factors / Q000096:biosynthesis*; D014471:Umbilical Veins / Q000166:cytology
D016588:Anticarcinogenic Agents; D003470:Culture Media; C494041:ETS1 protein, human; C494043:Ets1 protein, rat; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate