Gonadotropin-releasing hormone agonist inhibits estrone sulfatase expression of cystic endometriosis in the ovary.
OBJECTIVE
To clarify the inhibitory effect of GnRH agonist on estrone (E(1)) sulfatase expression.


METHODS
Retrospective immunohistochemical study.


METHODS
The Jikei University Hospital, Tokyo, Japan.


METHODS
Thirty-three women who had undergone cystectomy of the ovary or oophorectomy and were proved histopathologically to have cystic endometriosis in the ovary.


METHODS
Fifteen of the 33 patients were treated with GnRH agonists monthly for 2-6 months before surgery. The other 18 patients did not receive any hormonal therapy. Tissue sections were immunostained with an anti-E(1) sulfatase monoclonal antibody (KM1049) originating from human placenta.


METHODS
Microscopic evaluation to assess the presence and localization of E(1) sulfatase and to describe any variations in its expression with or without treatment with GnRH agonist.


RESULTS
Immunostaining showed that E(1) sulfatase was localized only on the glandular epithelial cells of cystic endometriosis in the ovary. The immunostaining with anti-E(1) sulfatase proved that GnRH agonist inhibited E(1) sulfatase expression in the cystic endometriosis in the ovary.


CONCLUSIONS
Gonadotropin-releasing hormone agonist inhibits E(1) sulfatase expression in cystic endometriosis in the ovary.
Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 3-25-8 Nihonbashi, Minato-ku, Tokyo 105-8461, Japan.
Kazuhiro
K
Maitoko
Hiroshi
H
Sasaki
D000328:Adult; D003692:Delayed-Action Preparations; D004715:Endometriosis / Q000188:drug therapy* / Q000201:enzymology / Q000601:surgery; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006801:Humans; D007150:Immunohistochemistry; D016729:Leuprolide / Q000627:therapeutic use*; D008875:Middle Aged; D010048:Ovarian Cysts / Q000188:drug therapy* / Q000201:enzymology / Q000601:surgery; D012189:Retrospective Studies; D013429:Sulfatases / Q000037:antagonists & inhibitors* / Q000378:metabolism
D003692:Delayed-Action Preparations; D004791:Enzyme Inhibitors; D007987:Gonadotropin-Releasing Hormone; D013429:Sulfatases; C020121:estrone sulfatase; D016729:Leuprolide