Molecular characterisation of streptomycin-resistant Mycobacterium tuberculosis strains isolated in Poland.
Primary drug resistance of Mycobacterium tuberculosis strains in Poland increased two-fold between 1997 and 2000. Among 3705 drug-resistant strains investigated in 2000, 169 were resistant to streptomycin alone or in combination with isoniazid, rifampicin and/or ethambutol. The molecular basis of streptomycin resistance for 88 (52%) of these strains in comparison with 15 susceptible controls was determined. The most prevalent mutation was the single substitution Lys43Arg in the rpsL gene, found in 30.7% of the strains analysed. However, as many as 51% of the strains investigated carried no mutation in the rpsL or rrs genes. The multiple mutations present in two Beijing family strains were also identified.
Brzostek
A
A
Centre for Medical Biology, Polish Academy of Sciences, Lodz, Poland.
Sajduda
A
A
Sliwiński
T
T
Augustynowicz-Kopeć
E
E
Jaworski
A
A
Zwolska
Z
Z
Dziadek
J
J
D000904:Antibiotics, Antitubercular; D004252:DNA Mutational Analysis; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D013307:Streptomycin
D000904:Antibiotics, Antitubercular; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin