Effects of serum from aplastic anemia patients on the expression of cyclin D3 isoform in umbilical cord blood CD34+ cells.
The pathogenesis of aplastic anemia (AA) was explored and the effects of AA serum on the expression of crucial cyclin D isoform (cyclin D3) in umbilical cord blood hematopoietic stem/progenitor cells were observed. The CD34+ cells were isolated from the cord blood with MIDI-MACS Semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; The expression level of cyclin D3 was assayed by semi-quantitative RT-PCR and Western-blot after the hematopoietic stem/progenitor cells were incubated in AA serum. The results showed that the AA serum could inhibit the formation of CFU-GM and down regulate the expression level of the cyclin D3 at the mRNA and protein level respectively. In conclusion, the AA serum could inhibit the proliferation of hematopoietic stem cells and down regulate level of cyclin D3, which might be one mechanism of hematopoiesis inhibition in AA.
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Fankai
F
Meng
Xiyou
X
Tan
Wenli
W
Liu
Hanying
H
Sun
Jianfeng
J
Zhou
Chunrui
C
Li
Dan
D
Liu
Li
L
He
Lan
L
Sun
D000741:Anemia, Aplastic / Q000097:blood*; D018952:Antigens, CD34 / Q000378:metabolism*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D056743:Cyclin D3; D016213:Cyclins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D044967:Serum
D018952:Antigens, CD34; C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins; D020033:Protein Isoforms; D012333:RNA, Messenger