Generation of Streptomyces globisporus SMY622 strain with increased landomycin E production and it's initial characterization.
Landomycin E (LaE) overproducing strain Streptomyces globisporus SMY6222 has been developed using UV induced mutagenesis and selection for streptomycin resistance. SMY622 has been shown by HPLC to produce 200-fold higher amounts of LaE when comparing with parental strain. The levels of transcription of regulatory gene lndI and oxygenase gene lndE are two times higher in the mutant than in the wild type. Gene rpsL for ribosomal protein S12 from SMY622 was shown to contain point mutation K43R. Possible reasons for increased LaE synthesis in SMY622 are discussed.
Department of Genetics and Biotechnology of Ivan Franko National University of L'viv, Grushevskogo st.4, L'viv 79005, Ukraine.
Oleksandr
O
Gromyko
Yuriy
Y
Rebets
Bohdan
B
Ostash
Andriy
A
Luzhetskyy
Masahiro
M
Fukuhara
Andreas
A
Bechthold
Tatsunosuke
T
Nakamura
Victor
V
Fedorenko
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016296:Mutagenesis; D010105:Oxygenases / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D012641:Selection, Genetic; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology
D000617:Aminoglycosides; D004269:DNA, Bacterial; D012269:Ribosomal Proteins; C113593:landomycin E; C034212:ribosomal protein S12; D010105:Oxygenases