An opaque-2-like transcription factor from pearl millet.
Pearl millet (Pennisetum glaucum L.), a species of the Poaceae family, is an important food crop in Africa, Asia and South America. Its nutritional value is due to storage prolamins accumulated in the seeds. In other species of the same family, the expression of the genes coding for storage prolamins is mediated by the regulatory protein opaque-2. In this paper we show that an opaque-2 -like protein is present in pearl millet too and is expressed during the early stages of seed development. The organization of the gene coding for this protein is similar to that of orthologous genes in other Poaceae species, i.e. six exons separated by five introns. A comparison of amino acid homologies with other described opaque-2 proteins is presented.
Embrapa-Recursos Genéticos e Biotecnologia--Cenargen, Parque Estacedil;ao Biológica--PqEB Final W5 Norte, 70770-900 Brasília--DF, Brazil.
Lucilia Helena
LH
Marcellino
Goran
G
Neshich
Antonio Furtado
AF
Neto
Eugen Silvano
ES
Gander
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D007438:Introns / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D031782:Pennisetum / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors