Expression and purification of the Bacillus subtilis thioredoxin superfamily protein YkvV.
Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thiol-disulfide oxidoreductase activity.
Tanaka
Ryoichi
R
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Araki
Yoko
Y
Mizukami
Makoto
M
Miyauchi
Akira
A
Ishibashi
Matsujiro
M
Tokunaga
Hiroko
H
Tokunaga
Masao
M
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013879:Thioredoxins / Q000378:metabolism*
D013879:Thioredoxins