Inhibition of cutaneous oxidative stress and two-stage skin carcinogenesis by Hemidesmus indicus (L.) in Swiss albino mice.
Chemopreventive potential of H. indicus on 7,12-dimethyl-benz[a]anthracene (DMBA)-initiated and 12-O-tetradecanoyl 13-phorbol acetate (TPA) promoted murine skin carcinogenesis has been assessed. Topical application of H. indicus resulted in significant protection against cutaneous tumorigenesis. Topical application of plant extract prior to that of TPA resulted in significant inhibition against TPA-caused induction of epidermal ornithine decarboxylase (ODC) activity and DNA synthesis. Application of H. indicus at a dose level of 1.5 and 3.0 mg/kg body weight in acetone prior to that of TPA treatment resulted in significant inhibition of oxidative stress. The level of lipid peroxidation was significantly reduced. In addition, depleted levels of glutathione and reduced activities of antioxidant enzymes were restored respectively). The results indicate that H. indicus is a potent chemopreventive agent in skin carcinogenesis.
Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi 110 062, India. sarwat786@rediffmail.com
Sarwat
S
Sultana
Aftab
A
Alam
Naghma
N
Khan
Sonia
S
Sharma
D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D031102:Hemidesmus; D015227:Lipid Peroxidation; D051379:Mice; D018384:Oxidative Stress; D012867:Skin / Q000201:enzymology / Q000378:metabolism*; D012878:Skin Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*
D002273:Carcinogens; D004247:DNA; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase