DKT1, a novel K+ channel from carrot, forms functional heteromeric channels with KDC1.
We report the isolation and characterisation of DKT1, a new carrot K+ channel alpha-subunit belonging to the Shaker-like family. DKT1 is expressed in many tissues of the adult plant, suggesting that it may play important roles in both nutrition and other important physiological processes. During embryo development, DKT1 is expressed at later phases implying the involvement of K+ in embryo maturation. When co-expressed with KDC1 in Xenopus oocytes, DKT1 is able to form functional, heteromeric channels, suggesting that possible interactions between these two ion channels in plant tissues may modulate K+ uptake.
CRIBI, Centro Interdipartimentale per le Biotecnologie Innovative, Università di Padova, Viale G. Colombo 3, 35131 Padova, Italy. elide@mail.cribi.unipd.it
Elide
E
Formentin
Serena
S
Varotto
Alex
A
Costa
Patrick
P
Downey
Monica
M
Bregante
Alessia
A
Naso
Cristiana
C
Picco
Franco
F
Gambale
Fiorella
F
Lo Schiavo
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018552:Daucus carota / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
D018076:DNA, Complementary; C419388:KDC1 protein, Daucus carota; D010940:Plant Proteins; D015221:Potassium Channels; D018749:RNA, Plant