Perforin expression in peripheral blood lymphocytes and skin-infiltrating cells in patients with lichen planus.
BACKGROUND
Current evidence suggests that lichen planus is a T-cell-mediated autoimmune disease in which cytotoxic mechanisms have been poorly investigated.


OBJECTIVE
We investigated the expression of perforin in subpopulations of peripheral blood lymphocytes (PBL) in exacerbation and remission phases of the disease as well as in skin lesions.


METHODS
We performed a simultaneous detection of perforin (intracellular molecule) and cell surface antigens on PBL by flow cytometry, and skin lesions were investigated by immunohistochemistry.


RESULTS
The most interesting finding was a significant increase of perforin expression in cytotoxic T lymphocytes (CD3+ perforin+ cells) in the exacerbation phase of disease (P < 0.05), which was mostly located in the CD8+ subpopulation (CD8+ perforin+) (P < 0.01). Using immunohistochemistry we confirmed the infiltration of T lymphocytes in skin lesions, especially of CD4+ and CD8+ phenotypes, compared with uninvolved (P < 0.05) and healthy skin (P < 0.01). The expression of perforin was also significantly higher in lesional skin compared with nonlesional and healthy skin (P < 0.05).


CONCLUSIONS
Our results clearly show the upregulation of perforin expression in peripheral blood as well as in lesions of patients with lichen planus and therefore suggest an important role for perforin in this autoimmune disease.
Department of Dermatovenerology, Medical Faculty, University of Rijeka, Kresimirova 42, HR-510000 Rijeka, Croatia.
L
L
Prpić Massari
M
M
Kastelan
F
F
Gruber
G
G
Laskarin
V
V
Sotosek Tokmadzić
N
N
Strbo
G
G
Zamolo
G
G
Zauhar
D
D
Rukavina
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007694:Killer Cells, Natural / Q000737:chemistry; D008010:Lichen Planus / Q000378:metabolism* / Q000473:pathology; D016131:Lymphocyte Subsets / Q000737:chemistry*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012867:Skin / Q000737:chemistry* / Q000473:pathology; D013601:T-Lymphocytes / Q000737:chemistry
D015703:Antigens, CD; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin