Ginkgo biloba extract (EGb761) and FK506 preserve energy metabolites in the striatum during focal cerebral ischemia and reperfusion in gerbils monitored by microdialysis.
Cell death after cerebral ischemia is mediated by the accumulation of excitatory amino acids, calcium influx into cells and the generation of free radicals. The aim of this study was to evaluate changes in energy-related metabolites in the striatum of gerbils subjected to focal cerebral ischemia after pretreatment with Ginkgo biloba extract (EGb761), a well-known antioxidant, and FK506, a calcium-dependent phosphatase calcineurin inhibitor. Ischemia was induced by occlusion of the right common carotid artery and the right middle cerebral artery for 60 min. A microdialysis probe was inserted into the right striatum to monitor extracellular glucose, lactate and pyruvate levels. This study showed decreases in glucose (10% of the baseline), pyruvate (20% of the baseline) and lactate (60% of the baseline), and a 5-fold increase in the lactate to pyruvate ratio during ischemia in the control group. Both EGb761 treatment and the combination (EGb761 and FK506) therapy significantly preserved glucose (50% of the baseline) and pyruvate (60% of the baseline) levels during ischemia. The marked increase in the lactate to pyruvate ratio was not observed in the combination group. These results suggest that preservation of cellular energy metabolism during cerebral ischemia and after restoration with reperfusion may contribute to the neuroprotective effects of EGb761 and FK506.
Lin
Jing-Ying
JY
Department of Medical Technology, Chung-Tai Institute of Health Sciences and Technology, Taichung Veterans General Hospital, Taichung, Taiwan, ROC. jylin020@ms21.hinet.net
Cheng
Fu-Chou
FC
Chung
Shu-Ying
SY
Lin
Ming-Cheng
MC
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000187:drug effects*; D005849:Gerbillinae; D020441:Ginkgo biloba; D005947:Glucose / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000188:drug therapy / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D017551:Microdialysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D011773:Pyruvates / Q000378:metabolism; D015424:Reperfusion; D016559:Tacrolimus / Q000627:therapeutic use*; D013997:Time Factors; D000096983:Ginkgo Extract
D005947:Glucose; D007773:Lactates; D010936:Plant Extracts; D011773:Pyruvates; D016559:Tacrolimus; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract