A recombinant luteinising-hormone-releasing-hormone immunogen bioeffective in causing prostatic atrophy.
Previous studies with a semi-synthetic vaccine indicated the utility of immunization against luteinising-hormone-releasing-hormone (LHRH) in prostate cancers. To overcome the limitations of the previous vaccine, which caused carrier induced suppression of antibody response on repeated immunizations and was costly to synthesize, two recombinant vaccines were designed, in which diptheria or tetanus toxoid used as carriers were replaced by 4-5 T non B peptides. The paper reports the immunogenecity, efficacy and safety of these multimer vaccines in rats, a homologous experimental animal. All animals generated anti-LHRH antibodies, which caused the decline of testosterone to castration levels at and above 0.15 OD units of antibody titres. The prostate was significantly atrophied in all animals immunized with these vaccines.
Talwar
G P
GP
The Talwar Research Foundation, E-8 Neb Valley, Neb Serai, New Delhi 110068, India. talwar37@hotmail.com
Raina
Komal
K
Gupta
Jagdish C
JC
Ray
Ruma
R
Wadhwa
S
S
Ali
Mushir M
MM
D000818:Animals; D001835:Body Weight / Q000502:physiology; D019496:Cancer Vaccines / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy; D008297:Male; D009929:Organ Size / Q000502:physiology; D011467:Prostate / Q000033:anatomy & histology / Q000254:growth & development / Q000276:immunology; D011471:Prostatic Neoplasms / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000276:immunology; D012671:Seminiferous Tubules / Q000166:cytology; D013739:Testosterone / Q000097:blood
D019496:Cancer Vaccines; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone