[Electrofusion of building coupling system for degrading anthracene and fusants' choices].
Some researches have been carried out for the electro fusion of building coupling system for degrading anthracene and the choices of fusants. During the experiments, total rate of fusion stable in alternation current frequency 500 kHz, impulse intensity 300 V/cm, impulse width 5 micros, amount of impulse 2 and alternation current intensity 20-40 V/cm were found. The resistant plate with erythromycin and ampicillin can screen the fusant of autochthonous bacterium and surfactant-producing bacterium. The resistant plate with anthracene and karnamicin can screen the fusant of autochthonous bacterium and efficient anthracene-degradation bacterium. The new fusants were found to have stable fluorescent characteristics.
Zhang
Jia-yao
JY
School of Environmental Science and Resource, Wuhan University, Wuhan 430072, China.
Ji
Miao
M
Fang
Cheng-xiang
CX
Chen
Fei-li
FL
Chen
Ling
L
Deng
Huan-huan
HH
D000873:Anthracenes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002459:Cell Fusion / Q000379:methods; D004560:Electricity; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D000873:Anthracenes; D012989:Soil Pollutants; C034020:anthracene