Toxicological, cellular and gene expression responses in earthworms exposed to copper and cadmium.
This study correlates sub-organismal changes with toxicological effects in earthworms (Lumbricus rubellus) exposed to copper and cadmium. Both metals reduced survival and reproduction at the highest concentration (LC50 5.11 microM Cu g(-1) and 4.04 microM Cd g(-1); cocoon production EC50s 5.17 microM Cu g(-1) and 1.86 microM Cd g(-1), all values as dry mass soil). Cadmium significantly reduced lysosomal membrane stability (at 1.86 microM Cd g(-1) and higher), upregulated metallothionein gene expression (at least sevenfold in all treatments) and reduced lysosome-associated-glycoprotein gene expression. Copper did not lower lysosomal membrane stability, but did upregulate metallothionein gene expression (at 2.5 microM Cu g(-1)), reduce lysosome-associated-glycoprotein gene expression and gave a nonlinear pattern for mitochondrial ribosomal subunit transcript expression (reduced at 0.35 and 0.811 microM Cu g(-1); higher at 2.5 microM Cu g(-1)). Correlation of metal body residue concentrations and cellular and molecular genetic responses with juvenile production rate confirmed a relationship for metallothionein expression, lysosomal membrane stability and cadmium tissue concentration in cadmium-exposed worms. Relationships between responses were also found for both metals. These suggested mechanisms for the interaction of cadmium and copper with specific gene products and with organelle (mitochondrial, lysosomal) functioning.
Centre for Ecology and Hydrology, Ecological Risk, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire PE28 2LS, UK. dasp@ceh.ac.uk
David J
DJ
Spurgeon
Stephen R
SR
Stürzenbaum
Claus
C
Svendsen
Peter K
PK
Hankard
A John
AJ
Morgan
Jason M
JM
Weeks
Peter
P
Kille
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D007928:Lethal Dose 50; D008247:Lysosomes / Q000187:drug effects; D008668:Metallothionein / Q000378:metabolism; D009835:Oligochaeta / Q000187:drug effects* / Q000502:physiology; D012313:RNA / Q000187:drug effects; D000077278:RNA, Mitochondrial; D012098:Reproduction / Q000187:drug effects; D012987:Soil
D000077278:RNA, Mitochondrial; D012987:Soil; D002104:Cadmium; D012313:RNA; D003300:Copper; D008668:Metallothionein