Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood.
OBJECTIVE
To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity.


METHODS
EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope. Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.


RESULTS
Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentration- and time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P<0.01).


CONCLUSIONS
Puerarin enhanced EPCs functional activity.
Zhu
Jun-Hui
JH
Department of Cardiovascular Diseases, the First Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310003, China.
Wang
Xing-Xiang
XX
Chen
Jun-Zhu
JZ
Shang
Yun-Peng
YP
Zhu
Jian-Hua
JH
Guo
Xiao-Gang
XG
Sun
Jian
J
D000071916:AC133 Antigen; D015703:Antigens, CD; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D007529:Isoflavones; C000606023:PROM1 protein, human; D010455:Peptides; D014665:Vasodilator Agents; D040301:Vascular Endothelial Growth Factor Receptor-2; C033607:puerarin