Immunohistochemical analysis of oxidative DNA damage in arsenic-related human skin samples from arsenic-contaminated area of China.
The appearance of 8-oxo-2'-deoxyguanosine (8-oxodG) was examined immunohistochemically using an 8-oxodG-monoclonal antibody in 28 cases of arsenic-related human skin tumors and in 20 cases of arsenic-unrelated human skin cancer to determine if the induction of oxidative stress participates in skin tumorigenesis caused by arsenics. The rate of 8-oxodG-positive was significantly higher in arsenic-related human skin cancer (28 of 28, 100%) than in arsenic-unrelated human skin cancer (3 of 20, 15%, P<0.01 by Chi2 test). Moreover, in all the arsenic-related skin samples, 8-oxodG was detected not only in tumor tissues but also in keratosis and normal tissues. These results suggest that the induction of oxidative stress may play an important role in arsenic carcinogenesis.
Department of Biochemical Toxicology, Nihon University, College of Pharmacy, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Yan
Y
An
Zenglin
Z
Gao
Zhongwen
Z
Wang
Shaohe
S
Yang
Jianfen
J
Liang
Yan
Y
Feng
Koichi
K
Kato
Masayuki
M
Nakano
Shoji
S
Okada
Kenzo
K
Yamanaka
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020261:Arsenic Poisoning / Q000150:complications*; D001706:Biopsy; D002681:China; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000506:poisoning*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012878:Skin Neoplasms / Q000209:etiology* / Q000235:genetics*
D004785:Environmental Pollutants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine