[A primary study on the gene expression profiling of human brain contusion by cDNA microarray].
OBJECTIVE
To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray.


METHODS
The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues.


RESULTS
Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1 (HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue.


CONCLUSIONS
The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.
Faculty of Forensic Medicine, Tongji Medical College, Huazfong University of Science and Technology, Wuhan 430030, China. yiwu@public.wh.hb.cn
Zhou
Z
Yi-wu
Zhang
Z
Yi-gu
Liu
L
Yan
D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D011519:Proto-Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D050720:Retinoblastoma-Like Protein p107 / Q000096:biosynthesis / Q000235:genetics
D005819:Genetic Markers; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D050720:Retinoblastoma-Like Protein p107