Substrate-dependent activation of thermolysin by salt.
Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at X M NaCl was expressed as 4.7(x) when [NaCl](o) < 0.5 M and 2.3(x) when [NaCl](o) > 0.5 M respectively.
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Hiroshi
H
Oneda
Yuko
Y
Muta
Kuniyo
K
Inouye
D001407:Bacillus / Q000201:enzymology*; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry*; D013820:Thermolysin / Q000378:metabolism*
D005456:Fluorescent Dyes; D012492:Salts; D012965:Sodium Chloride; D013820:Thermolysin