Influence of cetyltrimethylammonium bromide on phosphatidylcholine-coated capillaries.
Large unilamellar vesicles of egg-phosphatidylcholine (eggPC), a naturally occurring phospholipid, were used in capillary electrophoresis (CE) for semi-permanent coating of fused silica capillaries. The stability of the phospholipid coating was tested at different cetyltrimethylammonium bromide (CTAB) concentrations with and without CaCl(2) present in the coating solution. The effect of physical factors influencing the coating stability (e.g. duration of the coating time, storage temperature of the coating solution) were also studied. Standing overnight in background electrolyte (BGE) solution did not alter the eggPC phospholipid coating noticeably. The performance of the coating was tested with a mixture of basic proteins (lysozyme, ribonuclease A and alpha-chymotrypsinogen A). Highest efficiencies (over 200,000 plates m(-1)) were achieved when the capillary was filled for 15 h with a liposome solution containing both CTAB and CaCl(2).
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, PO Box 55, Helsinki 00014, Finland.
Ruth
R
Kuldvee
Maria V
MV
Lindén
Susanne K
SK
Wiedmer
Marja-Liisa
ML
Riekkola
D002122:Calcium Chloride / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D002593:Cetrimonium Compounds; D008081:Liposomes; D010713:Phosphatidylcholines; D002122:Calcium Chloride; D000077286:Cetrimonium