[Combined detection of CEA mRNA and CK 19 mRNA in peripheral blood cells of patients with lung cancer].
OBJECTIVE
To investigate the expression of CEA mRNA and CK(19) mRNA in peripheral blood cells of patients with lung cancer and evaluate its clinical significance.


METHODS
Peripheral blood nucleated cells of 50 patients with lung cancer were studied by RT-PCR to detect the expression of CEA mRNA and CK(19) mRNA.


RESULTS
The combined positive rate of CEA mRNA and CK(19) mRNA in patients with lung cancer (82.0%) was significantly higher than that in patients with benign lung diseases (28.0%) and healthy volunteers (8.1%) (P < 0.001). The expression rate had no relation to the clinical staging or histological type. Compared with single detection, combined detection increased the detection rate but did not decrease the specificity.


CONCLUSIONS
Combined detection of CEA mRNA and CK(19) mRNA expression in peripheral blood nucleated cells increase the sensitivity of detecting hematogenous dissemination of cancer cells. Long-term survival analysis and more specimens would be helpful for evaluating its clinical significance.
Department of Oncology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, China. lxfen21@21cn.com
Xu-fen
XF
Li
Yue
Y
Hu
Su-zhan
SZ
Zhang
Shu
S
Zheng
D000230:Adenocarcinoma / Q000097:blood* / Q000473:pathology; D000328:Adult; D000368:Aged; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000097:blood / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000473:pathology; D005260:Female; D006801:Humans; D007633:Keratins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002272:Carcinoembryonic Antigen; D012333:RNA, Messenger; D007633:Keratins