Biological laser printing of genetically modified Escherichia coli for biosensor applications.
One of the primary requirements of cell- or tissue-based sensors is the placement of cells and cellular material at or near the sensing elements of the device. The ability to achieve precise, reproducible and rapid placement of cells is the focus of this study. We have developed a technique, biological laser printing or BioLP, which satisfies these requirements and has advantages over current technologies. BioLP is capable of rapidly depositing patterns of active biomolecules and living cells onto a variety of material surfaces. Unlike ink jet or manual spotting techniques, this process delivers small volume (nl to fl) aliquots of biomaterials without the use of an orifice, thus eliminating potential clogging issues and enabling diverse classes of biomaterials to be deposited. This report describes the use of this laser-based printing method to transfer genetically-modified bacteria capable of responding to various chemical stressors onto agar-coated slides and into microtiter plates. The BioLP technology enables smaller spot sizes, increased resolution, and improved reproducibility compared to related technologies.
Barron
J A
JA
Biological Chemistry Section, Chemistry Division, Chemical Dynamics and Diagnostics Branch, Naval Research Laboratory, Bldg. 207, 4555 Overlook Avenue SW, Washington, DC 20375, USA.
Rosen
R
R
Jones-Meehan
J
J
Spargo
B J
BJ
Belkin
S
S
Ringeisen
B R
BR
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D016287:Computer Peripherals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D007834:Lasers; D009268:Nalidixic Acid / Q000032:analysis* / Q000494:pharmacology*; D011327:Printing / Q000295:instrumentation* / Q000379:methods
D009268:Nalidixic Acid