Loss of function mechanism in aprataxin-related early-onset ataxia.
Early-onset ataxia with ocular motor apraxia and hypoalbuminemia is an autosomal recessive form of cerebellar ataxia that occurs most commonly in Japan but is also frequently seen in Europe. This disease is caused by mutations in the aprataxin gene, but the functions of the gene product and the pathogenic mechanism remain unclear. The present study provides experimental evidence that the histidine triad (HIT) domain in aprataxin has enzymatic activity that is negatively regulated by the intramolecular interaction of the N-terminal domain. Furthermore, the reduction in HIT activity seen in all the disease-causing mutants tested, and the correlation between the reduced activity and the severe phenotype, support that aprataxin's physiological function is associated with its catalytic activity. Our findings suggest that the clinical phenotypes are caused by a loss of aprataxin function, attributable largely to diminished HIT activity but partially to a reduction in the levels of gene products.
Hirano
Makito
M
Department of Neurology, Nara Medical University, Nara, Japan. hirano_makito@yahoo.co.jp
Furiya
Yoshiko
Y
Kariya
Shingo
S
Nishiwaki
Tomohisa
T
Ueno
Satoshi
S
D017766:Acid Anhydride Hydrolases / Q000235:genetics; D017668:Age of Onset; D001259:Ataxia / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017385:Sequence Homology; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase; C438188:APTX protein, human