Lipid-mediated light activation of a mechanosensitive channel of large conductance.
This paper describes the reversible activation of a mechanosensitive channel via a light-sensitive lipid mimic. For these experiments, the mechanosensitive channel of large conductance (MscL) protein from Lactococcus lactis and Escherichia coli was reconstituted in lipid bilayers composed of 80 mol % 1,2-dioleoyl-sn-glycero-3-phosphocholine and 20 mol % di-(5-[[4-(4-butylphenyl)azo]phenoxy]pentyl)phosphate (4-Azo-5P). Light-induced isomerization of the azobenzene moiety of 4-Azo-5P from trans to cis was used to activate MscL.
Folgering
Joost H A
JH
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Kuiper
Johanna M
JM
de Vries
Alex H
AH
Engberts
Jan B F N
JB
Poolman
Bert
B
D001391:Azo Compounds / Q000737:chemistry / Q000528:radiation effects; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology / Q000528:radiation effects; D004926:Escherichia coli / Q000737:chemistry / Q000502:physiology; D007473:Ion Channels / Q000737:chemistry* / Q000502:physiology / Q000528:radiation effects*; D013294:Lactococcus lactis / Q000737:chemistry / Q000502:physiology; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry / Q000528:radiation effects; D008055:Lipids / Q000737:chemistry* / Q000528:radiation effects*; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000528:radiation effects; D010713:Phosphatidylcholines / Q000737:chemistry / Q000528:radiation effects; D013237:Stereoisomerism; D013499:Surface Properties; D013997:Time Factors
D001391:Azo Compounds; D001426:Bacterial Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008055:Lipids; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; C510725:di-(5-((4-(4-butylphenyl)azo)phenoxy)pentyl)phosphate; C017251:1,2-oleoylphosphatidylcholine