Establishment of an ex vivo model of monocytes-derived macrophages differentiated from peripheral blood mononuclear cells (PBMCs) from HIV-1 transgenic rats.
It has been developed an HIV type 1 transgenic rat model (HIV-1 Tg Rat) which contains a gag-pol-deleted HIV-1 provirus regulated by the viral LTR promoter. Although it harbors a non infectious provirus, efficient viral expression occurs in different tissues and disease manifestations as well as immune-response alterations and pathologies similar to humans can be observed. Regulation of HIV-1 expression is influenced by various cellular factors and it is well known that macrophages are one of the major reservoir of HIV-1 infection and a vehicle for virus spread to other tissues. Purpose of our work was to establish an antigen presenting cells (monocyte-derived macrophages, MDM) ex vivo model from these HIV-1 transgenic rats. This model can be used to study function of HIV-infected MDM and their behavior like HIV-1 reservoir. Ultimately, these studies may be helpful in defining approaches to control HIV-1 spread.
Division of Basic Science, Institute of Human Virology, University of Maryland, 725 W Lombard St, Baltimore MD, 21201, USA.
Renata
R
Mazzucchelli
Marialaura
M
Amadio
Sabrina
S
Curreli
Frank
F
Denaro
Kristi
K
Bemis
William
W
Reid
Joseph
J
Bryant
Agostino
A
Riva
Massimo
M
Galli
Davide
D
Zella
D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000502:physiology*; D005822:Genetic Vectors; D015497:HIV-1; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D008954:Models, Biological; D051381:Rats