A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1.
Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.
Nisole
Sébastien
S
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Lynch
Clare
C
Stoye
Jonathan P
JP
Yap
Melvyn W
MW
D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae / Q000235:genetics*; D001483:Base Sequence; D036022:Capsid Proteins / Q000378:metabolism; D002874:Chromosome Mapping; D021984:Cyclophilin A / Q000235:genetics*; D015497:HIV-1 / Q000254:growth & development*; D007668:Kidney / Q000378:metabolism*; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases
D036022:Capsid Proteins; D011506:Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases; D021984:Cyclophilin A