Preparative isolation and purification of syringin and edgeworoside C from Edgeworthia chrysantha Lindl by high-speed counter-current chromatography.
The bioactive compound syringin along with edgeworoside C were separated from the n-butanol extract of the stems and barks of Edgeworthia chrysantha Lindl (E. papyrifera) by high-speed counter-current chromatography (HSCCC) while it was difficult to purify each compound by silica gel column chromatography. Syringin was isolated from this plant for the first time. The two-phase solvent system used was composed of ethyl acetate-ethanol-water at an optimized volume ratio of 15:1:15 (v/v/v). Preparative HSCCC yielded, from 110mg of the partially purified extract, 28mg of syringin and 45 mg edgeworoside C each at over 96% purity by high-performance liquid chromatography analysis. Their structures were identified by electron impact ionization MS, 1H NMR and 13C NMR.
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. yanjizhong@hzcnc.com
Jizhong
J
Yan
Shengqiang
S
Tong
Jianjun
J
Chu
Liuqing
L
Sheng
Gang
G
Chen
D003374:Coumarins / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D009005:Monosaccharides / Q000302:isolation & purification*; D010666:Phenylpropionates / Q000302:isolation & purification*; D029645:Thymelaeaceae / Q000737:chemistry*
D003374:Coumarins; D005960:Glucosides; D009005:Monosaccharides; D010666:Phenylpropionates; C496796:edgeworoside C; C028305:syringin