Extracellular matrix family proteins that are potential targets of Dd-STATa in Dictyostelium discoideum.
Dd-STATa is a functional Dictyostelium homologue of metazoan STAT (signal transducers and activators of transcription) proteins, which is activated by cAMP and is thereby translocated into the nuclei of anterior tip cells of the prestalk region of the slug. By using in situ hybridization analyses, we found that the SLF308 cDNA clone, which contains the ecmF gene that encodes a putative extracellular matrix protein and is expressed in the anterior tip cells, was greatly down-regulated in the Dd-STATa-null mutant. Disruption of the ecmF gene, however, resulted in almost no phenotypic change. The absence of any obvious mutant phenotype in the ecmF-null mutant could be due to a redundancy of similar genes. In fact, a search of the Dictyostelium whole genome database demonstrates the existence of an additional 16 homologues, all of which contain a cellulose-binding module. Among these homologues, four genes show Dd-STATa-dependent expression, while the others are Dd-STATa-independent. We discuss the potential role of Dd-STATa in morphogenesis via its effect on the interaction between cellulose and these extracellular matrix family proteins.
Shimada
Nao
N
Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Nishio
Keiko
K
Maeda
Mineko
M
Urushihara
Hideko
H
Kawata
Takefumi
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D000242:Cyclic AMP / Q000502:physiology; D004023:Dictyostelium / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D017353:Gene Deletion; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
D016326:Extracellular Matrix Proteins; D015800:Protozoan Proteins; D015534:Trans-Activators; D000242:Cyclic AMP