DNA-functionalized nanotube membranes with single-base mismatch selectivity.
We describe synthetic membranes in which the molecular recognition chemistry used to accomplish selective permeation is DNA hybridization. These membranes contain template-synthesized gold nanotubes with inside diameters of 12 nanometers, and a "transporter" DNA-hairpin molecule is attached to the inside walls of these nanotubes. These DNA-functionalized nanotube membranes selectively recognize and transport the DNA strand that is complementary to the transporter strand, relative to DNA strands that are not complementary to the transporter. Under optimal conditions, single-base mismatch transport selectivity can be obtained.
Kohli
Punit
P
Department of Chemistry and Center for Research at the Bio/Nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
Harrell
C Chad
CC
Cao
Zehui
Z
Gasparac
Rahela
R
Tan
Weihong
W
Martin
Charles R
CR
D020137:Base Pair Mismatch; D001692:Biological Transport; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006046:Gold; D008567:Membranes, Artificial; D043942:Nanotubes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization
D008567:Membranes, Artificial; D006046:Gold; D004247:DNA; C085497:endodeoxyribonuclease SfcI; D015252:Deoxyribonucleases, Type II Site-Specific