Experimental bile-duct ligation resulted in accumulation of oxidized low-density lipoproteins in BALB/c mice liver.
OBJECTIVE
Oxidized low-density lipoproteins (LDL), which are produced during oxidative stress by the process of lipid peroxidation, have also been proposed to have complex roles in many other immuno-inflammatory mechanisms. It has been shown that bile-duct ligation results in oxidative stress in the liver of animals. The aim of this study was to investigate if oxidized LDL are produced in the liver tissues of bile-duct-ligated mice.


METHODS
Obstructive jaundice was induced in BALB/c mice by the ligation and division of the common bile duct. Liver concentrations of glutathione and malondialdehyde were measured in the sham-operated (n = 10) and bile-duct-ligated (n = 10) mice on the 10th day of obstructive jaundice. The presence of oxidized LDL in the liver tissue sections was evaluated using a special, novel immunofluorescent staining method. The final step was to explore the existence of oxidized LDL under fluorescent microscopy.


RESULTS
Compared with sham-operated mice, jaundiced mice showed significantly higher levels of malondialdehyde and lower concentrations of reduced glutathione in the liver. While there was no staining in the sham-operated group, bile-duct ligation resulted in positive oxidized LDL staining in the liver tissues of mice. The present study testifies that bile-duct ligation results in oxidative stress and enhanced lipid peroxidation in the hepatic tissues of BALB/c mice and moreover, that oxidized LDL accumulate in the liver of mice with experimental obstructive jaundice.


CONCLUSIONS
Oxidized LDL may be an important and direct indicator of ongoing oxidative stress and enhanced lipid peroxidation in obstructive jaundice. The potential roles of this finding were also discussed, briefly.
Department of Surgery, School of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey. mustafacomert@hotmail.com
Mustafa
M
Cömert
Ishak Ozel
IO
Tekin
Serefden
S
Acikgöz
Yücel
Y
Ustündağ
Bulent H
BH
Uçan
Zeki
Z
Acun
Figen
F
Barut
Vildan
V
Sümbüloğlu
D000818:Animals; D001652:Bile Ducts; D005978:Glutathione / Q000378:metabolism; D041781:Jaundice, Obstructive / Q000378:metabolism*; D008026:Ligation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008111:Liver Function Tests; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D008315:Malondialdehyde; D005978:Glutathione