Effects of periconceptional undernutrition on the initiation of parturition in sheep.
In sheep, parturition is initiated by increased fetal hypothalamic-pituitary-adrenal axis (HPAA) activity leading to PGE(2) and PGF(2alpha) production and a rise in the 17beta-estradiol-progesterone (E(2)/P(4)) ratio. Uteroplacental PG production can also increase fetal HPAA activity. Periconceptional maternal undernutrition accelerates fetal HPAA maturation resulting in preterm labor. We determined whether preterm labor was preceded by an increase in PG concentrations and E(2)/P(4) ratio and whether these increases preceded or followed the corresponding rise in cortisol concentrations. Singleton-bearing ewes were nourished ad libitum (N, n = 9) or undernourished (UN, n = 10) to reduce maternal weight by 15% from -61 days (d) to +30 d after mating with ad libitum intake thereafter. Paired maternal and fetal blood samples were collected from 126 d until delivery. Half the UN group delivered prematurely (>2 SD below mean gestation for the flock). PG and cortisol concentrations and E(2)/P(4) ratio increased before delivery in the same way in both groups. However, the increases occurred 7-10 d earlier in UN than in N animals. In both UN and N fetuses cortisol concentrations rose before fetal and maternal PG concentrations and maternal E(2)/P(4) ratio. Periconceptional maternal undernutrition induces preterm delivery in sheep by advancing the expected prepartum rise in cortisol and PG concentrations and E(2)/P(4) ratio. The rise in fetal cortisol concentration precedes the rise in fetal and maternal PG concentrations and maternal E(2)/P(4) ratio, suggesting that the underlying mechanism is likely to be acceleration of fetal HPAA maturation, resulting in initiation of the normal process of parturition.
Liggins Institute, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Vasumathy
V
Kumarasamy
Murray D
MD
Mitchell
Frank H
FH
Bloomfield
Mark H
MH
Oliver
Melanie E
ME
Campbell
John R G
JR
Challis
Jane E
JE
Harding
D000818:Animals; D000831:Animals, Newborn / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005508:Food Deprivation; D005865:Gestational Age; D036801:Parturition / Q000502:physiology*; D011247:Pregnancy; D047928:Premature Birth / Q000503:physiopathology; D039401:Prenatal Nutritional Physiological Phenomena / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D012756:Sheep
C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost; D015232:Dinoprostone