Recombinant fraction 1 protein of Yersinia pestis activates murine peritoneal macrophages in vitro.
Fraction 1 antigen of Yersinia pestis is a capsule protein of 17.5kDa, known to induce thymocyte proliferation and have anti-phagocytic role in macrophages. It serves as a major protective antigen against challenge of Y. pestis by inducing high concentration of IgG1 antibody response. In the present investigation it is observed that 10microg/ml of rF1 antigen activated murine peritoneal macrophages in vitro. rF1 induced the production of TNF-alpha, IL-1, IL-6, and NO. rF1 treatment also induced increased transcription of IFN-gamma and its related chemokines KC, IP-10, MIP-1alpha, MIP-1beta, MCP-1, RANTES in macrophages. Significantly increased transcription of TLR5 was observed in macrophages treated with rF1, while the expression of TLR2 and TLR4 remained unaffected.
Sodhi
Ajit
A
School of Biotechnology, Banaras Hindu University, Varanasi 221 005, UP, India. ajit.sodhi@lycos.com
Sharma
Rajesh Kumar
RK
Batra
H V
HV
Tuteja
Urmil
U
D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism; D008262:Macrophage Activation / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015010:Yersinia pestis / Q000378:metabolism*
D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D012273:Ribulose-Bisphosphate Carboxylase