Identification of a neutralizing epitope in the betaE-betaF loop of VP1 of equine rhinitis A virus, defined by a neutralization-resistant variant.
Equine rhinitis A virus strain 393/76 (ERAV.393/76) was passaged in the presence of post-infection ERAV.393/76 equine polyclonal antiserum (EPA). Viruses with increased resistance to neutralization by EPA were obtained after 15 passages. Compared with the parent virus, five plaque-purified, neutralization-resistant mutant viruses, in addition to the non-plaque-purified viruses that were examined, had a Glu-->Lys change at position 658, which is located in the predicted betaE-betaF (EF) loop of VP1. Rabbit antiserum was prepared against the isolated EF loop of ERAV.393/76 VP1 expressed as a fusion protein with glutathione S-transferase. This antiserum bound to purified ERAV.393/76 in Western blots, but not to the neutralization-resistant mutant virus or to ERAV.PERV/62, a naturally occurring ERAV strain that has a Lys residue at position 658. These results suggest that the EF loop of VP1 is involved in a neutralization epitope of ERAV.
Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia.
Rachel A
RA
Stevenson
Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia.
Jin-An
JA
Huang
Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia.
Michael J
MJ
Studdert
Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia.
Carol A
CA
Hartley
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000032:analysis; D016679:Genome, Viral; D005973:Glutamine / Q000737:chemistry; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008239:Lysine / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D014709:Vero Cells
D036022:Capsid Proteins; D000939:Epitopes; D005973:Glutamine; D008239:Lysine