Upregulation and protein trafficking of aquaporin-2 attenuate cold-induced osmotic damage during cryopreservation.
Aquaporins (AQPs) are a recently discovered family of proteins that function as transmembrane water channels. These proteins regulate the delicate osmotic balance across the cell plasma membrane. Given that osmotic damage is the major contributing factor to cell death during freezing, we hypothesized that regulation of AQPs may have an unrealized role in protecting cells from osmotic damage during cryopreservation. Rat kidney inner medullar collecting duct (IMCD) cells were treated with arginine vasopressin (AVP) to increase the amount of AQP2 in the external plasma membrane before freezing in University of Wisconsin solution at -4 degrees C for 24 h. This resulted in a significant increase in cell viability on warming. Conversely, treatment of IMCD cells with AVP and W7 (which inhibits AQP2 protein trafficking to the plasma membrane) before freezing resulted in a 55% decrease in cell viability. These preliminary data indicate that regulation of AQP2 can attenuate cold-induced osmotic damage in rat kidney IMCD cells.
Wang
Wenjun
W
Department of Chemistry, SUNY-Binghamton, Binghamton, New York 13902, USA.
Ben
Robert N
RN
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D051399:Aquaporin 2; D020346:Aquaporins / Q000378:metabolism*; D001127:Arginine Vasopressin / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D003080:Cold Temperature; D015925:Cryopreservation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione; D007328:Insulin; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D021381:Protein Transport; D011887:Raffinose; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology; D015854:Up-Regulation; D014665:Vasodilator Agents / Q000494:pharmacology
C496025:Aqp2 protein, rat; D051399:Aquaporin 2; D020346:Aquaporins; D004791:Enzyme Inhibitors; D007328:Insulin; D019188:Organ Preservation Solutions; D013449:Sulfonamides; C055206:University of Wisconsin-lactobionate solution; D014665:Vasodilator Agents; D001127:Arginine Vasopressin; D000493:Allopurinol; C017967:W 7; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose