Surface effects on PCR reactions in multichip microfluidic platforms.
We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Founders Pavilion, Philadelphia, PA 19104, USA.
Nicholas J
NJ
Panaro
Xing Jian
XJ
Lou
Paolo
P
Fortina
Larry J
LJ
Kricka
Peter
P
Wilding
D004867:Equipment Design; D019544:Equipment Failure Analysis / Q000379:methods*; D008422:Materials Testing / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010969:Plastics / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties
D010969:Plastics