In vitro evaluation of the antimicrobial activity of five root canal sealers.
The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.
Department of Restorative Dentistry, Area of Endodontics, Dental School of Piracicaba, UNICAMP, Piracicaba, SP, Brazil. bpgomes@fop.unicamp.br
Brenda Paula Figueiredo de Almeida
BP
Gomes
José Assis
JA
Pedroso
Rogério Castilho
RC
Jacinto
Morgana Eli
ME
Vianna
Caio Cezar Randi
CC
Ferraz
Alexandre Augusto
AA
Zaia
Francisco José
FJ
de Souza-Filho
D000190:Actinomyces / Q000187:drug effects; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001729:Bismuth / Q000008:administration & dosage / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000008:administration & dosage / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D004853:Epoxy Resins / Q000008:administration & dosage / Q000494:pharmacology; D005557:Formaldehyde / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology; D008422:Materials Testing; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013053:Spectrophotometry; D013211:Staphylococcus aureus / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D013943:Thymol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors
D000890:Anti-Infective Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C080620:Sealer 26; C011244:corticosteroid methanetriol mixture; C534916:epoxy resin-based root canal sealer; C074417:Endo-Fill; D005557:Formaldehyde; D013943:Thymol; D003907:Dexamethasone; D002126:Calcium Hydroxide; D001729:Bismuth; D006854:Hydrocortisone