Stable isotope labeling of Arabidopsis thaliana for an NMR-based metabolomics approach.
Nuclear magnetic resonance (NMR) will become a key technology in plant metabolomics with the use of stable isotope labeling and advanced hetero-nuclear NMR methodologies. To demonstrate the power of this approach, we performed multi-dimensional hetero-nuclear NMR analysis of metabolic movement of carbon and nitrogen nuclei in Arabidopsis thaliana. First, distinct ethanol-stress response was investigated using (13)C-labeled wild type and an ethanol-hypersensitive mutant plants. Furthermore, we followed nitrogen fluxes in (15)N-labeled seeds during the initiation of germination in vivo. The future role of stable isotope-labeling combined with advanced hetero-nuclear NMR in plant metabolomics is discussed.
Kikuchi
Jun
J
Protein Research Group, Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro, Tsurumi, Yokohama, 230-0045 Japan.
Shinozaki
Kazuo
K
Hirayama
Takashi
T
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology; D018525:Germination / Q000187:drug effects / Q000235:genetics; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D009587:Nitrogen Isotopes / Q000032:analysis / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D000431:Ethanol