title: Universal labeling chemistry for nucleic acid detection on DNA chips.
abstract: An efficient strategy for nucleic acid labeling and analysis on deoxyribonucleic acid (DNA) chips has been developed. This approach, which combines the fragmentation and the labeling steps, is based on the reactivity of the phosphates of DNA and ribonucleic acid (RNA) fragments and is using reporter molecules bearing a bromomethyl- or aryldiazomethane-reactive group. In this chapter, we describe the preparation of the reactive label and protocols for efficient labeling of any nucleic acid sequence, DNA or RNA, prior to their hybridization, detection, and analysis on DNA chips.
authors.affiliation: Advanced Technology/Molecular Diagnostics, BioMérieux, Marcy L'Etoile, France.
authors.forename: Ali
authors.initials: A
authors.lastname: Laayoun
authors.forename: Eloy
authors.initials: E
authors.lastname: Bernal-Méndez
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sothier
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Kotera
authors.forename: Alain
authors.initials: A
authors.lastname: Troesch
mesh_terms: D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D009696:Nucleic Acids