Reliability of intraocular pressure by Tono-Pen XL over amniotic membrane patch in human.
OBJECTIVE
To determine the reliability of Tono-Pen XL in measuring intraocular pressure (IOP) over the amniotic membrane patch in human eyes.


METHODS
Ten healthy volunteers (nine males and one female) participated in this study. After topical anesthesia, IOP was measured using the Tono-Pen XL before and after the application of a single-layer amniotic membrane patch in the right eye and double-layer amniotic membrane patch in the left eye.


RESULTS
We found no statistical difference between the IOP measured with and without a single layer amniotic membrane (P = 0.808). Nor did we find any statistical difference between the IOP measured with and without double-layer amniotic membrane patch (P = 0.813). The average thickness of the single- and the double-layer amniotic membrane patch measured by pachymetry was 131.9 +/- 31.4 microm (mean +/- SD) and was 246.1 +/- 69.3 microm (mean +/- SD), respectively.


CONCLUSIONS
This study showed that it is reliable to measure IOP by Tono-Pen XL over single or a double layer of amniotic membrane patch on healthy human eyes. Further studies are required to determine the accuracy of IOP measurement over amniotic membrane patch on diseased corneas.
Yoshita
Tsuyoshi
T
Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Kobayashi
Akira
A
Takahashi
Mami
M
Sugiyama
Kazuhisa
K
D000328:Adult; D001684:Biological Dressings; D005260:Female; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000295:instrumentation*