[Development of recombinant human-derived IgG antibody against HBsAg].
BACKGROUND
To investigate preparation of human-derived anti-HBs IgG antibody.


METHODS
mRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test.


RESULTS
A whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant.


CONCLUSIONS
It can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.
Guo
Yu
Y
Laboratory of Hepatitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Liang
Mi-fang
MF
Bi
Sheng-li
SL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D006510:Hepatitis B Antibodies / Q000096:biosynthesis* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011994:Recombinant Proteins