[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth].
OBJECTIVE
To evaluate the efficiency of marker gene and reporter gene transfection into human squamous cell carcinoma of larynx implanted in nude mice with a new EGFR mediated non-viral polypeptide gene delivery system.


METHODS
Labeled streptavidin biotin (LSAB) was applied to examine over-expression of epidermal growth factor receptor (EGFR) on the squamous cell carcinoma of larynx. The complex of marker gene or reporter gene and gene delivery system was prepared and subsequently inoculated with Hep 2 cells or injected into laryngeal carcinoma implanted on nude mice.


RESULTS
Over-expression of EGFR was observed on inoculated Hep 2 cells and on 65% of laryngeal carcinoma specimen examined (15/23). Positive staining was located on the surface of the cells and some of them in the cytoplasma. Green fluorescence was observed on Hep 2 cells under fluorescent microscope 48 hrs after PEGFP-C1 gene transfection and became the strongest 72 hrs after transfection. The rate of transfection was 80% (78/97). Blue staining was noticed 12 hrs after beta-gal gene transfer and it became more remarkable 24 hrs after gene transfection with X-gal staining on the implanted human laryngeal carcinoma on nude mice.


CONCLUSIONS
Squamous cell carcinoma of head and neck over-expressed EGFR and a non-viral GE7 polypeptide gene delivery system could transfer exogenous genes into laryngeal carcinoma cells and tissues with high efficiency and targeting both in vitro and in vivo suggesting that GE7 would be applicable as a new targeted, high efficient gene delivery system to the gene therapy of squamous cell carcinoma of head and neck.
Xiao
Shui-fang
SF
Department of Otorhinolaryngology-Head and Neck Surgery, First Hospital of Peiking University, Beijing 100034, China. xsfxdd@public.bta.net.cn
Xi
Lin
L
Zhou
Yi-ding
YD
Wang
Cheng-yuan
CY
Tian
Pei-kun
PK
Gu
Jian-ren
JR
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D051379:Mice; D009368:Neoplasm Transplantation; D010455:Peptides / Q000235:genetics; D014162:Transfection
D010455:Peptides; D066246:ErbB Receptors