Separation of polyethylene glycol-modified proteins by open tubular capillary electrochromatography.
This study involves the characterization of six polyethylene glycol-modified proteins by open tubular capillary electrochromatography, a high-resolution, versatile and reproducible technique for the analysis of biomolecules and pharmaceuticals. Optimized conditions were obtained with respect to type of capillary modification (cholesterol and octadecyl), applied voltage (+20 and -20 kV), buffer pH (2.14-8.14) and addition of methanol modifier to the mobile phase. Electrochromatograms were obtained with both cathodic and anodic applied electric fields. In the case of one PEG-protein, superoxide dismutase, a comparison was made to a previous study. Reproducibility and column lifetime were also evaluated in assessing the usefulness of the method.
Pesek
Joseph J
JJ
Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. pesek@sjsu.edu
Matyska
Maria T
MT
Krishnamoorthi
Vidhya
V
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D011092:Polyethylene Glycols