An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid.
An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 mmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 micro mol/L, where the concentration of Fe(II) was 50 micro mol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).
Linxiang
Li
L
Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.
Abe
Yoshihiro
Y
Nagasawa
Yukiko
Y
Kudo
Risa
R
Usui
Noriko
N
Imai
Kazuhiro
K
Mashino
Tadahiko
T
Mochizuki
Masataka
M
Miyata
Naoki
N
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000032:analysis*; D006900:Hydroxylation; D010795:Phthalic Acids / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013481:Superoxides / Q000737:chemistry
D010795:Phthalic Acids; D013481:Superoxides; D017665:Hydroxyl Radical; C011363:terephthalic acid; D006861:Hydrogen Peroxide