title: [Relationship of polycyclic aromatic hydrocarbon exposure with peripheral blood lymphocyte DNA damage in coke oven workers].
abstract: OBJECTIVE
  To investigate the relationship between lymphocyte DNA damage and polycyclic aromatic hydrocarbons (PAHs) exposure in coke oven workers.
  
  
  METHODS
  Two hundred and thirty-five coke oven workers and 30 controls were selected in this study. Alkaline single-cell gel electrophoresis was used to evaluate the lymphocyte DNA damage, HPLC was employed to measure 1-hydroxypyrene levels in spot urine samples which were obtained at the end of a workweek (4 days of 8 hours/day) and personal information including occupational exposure, age, sex, smoking and drinking status was collected by the questionnaire.
  
  
  RESULTS
  The lymphocyte DNA damage level expressed as olive moment in coke oven workers was significantly higher than that of controls [2.47 (0.22 approximately 46.68) vs 0.94 (0.42 approximately 4.21), P < 0.01], and correlation between urinary 1-hydroxypyrene concentrations and olive moment was found (Spearman Partial correlation coefficient = 0.22, P < 0.01) in coke oven workers. The 1.9 of olive moment value was used as the limit to determine whether the subject DNA damage was positive. The coke oven workers had significantly higher risk in DNA damage (adjusted OR = 5.38, 95% CI = 2.07 approximately 14.08) than did controls, and dose-response relationships were also found between external exposure (exposure category) or internal doses (urinary 1-hydroxypyrene) and DNA damage.
  
  
  CONCLUSIONS
  There are dose-effect and dose-response relationships between PAHs exposure and lymphocyte DNA damage in coke oven workers.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Leng
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Ye-Ping
authors.initials: YP
authors.lastname: Gu
authors.forename: Wen-Zhong
authors.initials: WZ
authors.lastname: Zhang
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Li
authors.forename: Zu-Fei
authors.initials: ZF
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Zhong-Xu
authors.initials: ZX
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Feng-Sheng
authors.initials: FS
authors.lastname: He
mesh_terms: D000328:Adult; D000818:Animals; D003077:Coke / Q000009:adverse effects*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D011721:Pyrenes / Q000032:analysis / Q000378:metabolism; D011795:Surveys and Questionnaires
chemical_list: D003077:Coke; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C033146:1-hydroxypyrene