title: Gram-positive merA gene in gram-negative oral and urine bacteria.
abstract: Clinical mercury resistant (Hg(r)) Gram-negative bacteria carrying Gram-positive mercury reductase (merA)-like genes were characterized using DNA-DNA hybridization, PCR and sequencing. A PCR assay was developed which discriminated between the merA genes related to Staphylococcus and those related to the Bacillus/Streptococcus merA genes by the difference in size of the PCR product. DNA sequence analysis correlated with the PCR assay. The merA genes from Acinetobacter junii, Enterobacter cloacae and Escherichia coli were sequenced and shared 98-99% identical nucleotide (nt) and 99.6-100% amino acid identity with the Staphylococcus aureus MerA protein. A fourth merA gene, from Pantoeae agglomerans, was partially sequenced (60%) and had 99% identical nt and 100% amino acid identity with the Streptococcus oralis MerA protein. All the Hg(r) Gram-negative bacteria transferred their Gram-positive merA genes to a Gram-positive Enterococcus faecalis recipient with the resulting transconjugants expressing mercury resistance. These Gram-positive merA genes join Gram-positive tetracycline resistance and Gram-positive macrolide resistance genes in their association with mobile elements which are able to transfer and express in Gram-negative bacteria.
authors.affiliation: University of Washington, Seattle, WA 98195, USA.
authors.forename: Kayode K
authors.initials: KK
authors.lastname: Ojo
authors.forename: Diane
authors.initials: D
authors.lastname: Tung
authors.forename: Henrique
authors.initials: H
authors.lastname: Luis
authors.forename: Mario
authors.initials: M
authors.lastname: Bernardo
authors.forename: Jorge
authors.initials: J
authors.lastname: Leitao
authors.forename: Marilyn C
authors.initials: MC
authors.lastname: Roberts
mesh_terms: D000595:Amino Acid Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics*; D008628:Mercury / Q000494:pharmacology; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014556:Urine / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010088:Oxidoreductases; C021551:mercuric reductase; D008628:Mercury