Is mRNA and protein level of CD46 altered in measles virus vaccine strain S191-infected cells?
Previous research showed that the expression of measles virus receptor CD46 was downregulated after expression of measles virus hemagglutinin protein on the surface of the virus infected cell or triggered by infected cell-to-cell contact. We reported here that the mRNA level of CD46 in MV infected cells was not changed which was tested by real-time quantitative PCR. To further analyse the surface expression alteration of CD46 after MV infection, flow cytometric analysis and indirect immunofluorescence were used to detect the protein level of CD46. Altogether, our results provided a demonstration that the expression of CD46 was not downregulated by the infection of MV strain S191 both on mRNA level and cellular surface protein level. Previous results reported that the "downregulation" of CD46 expression on the cell surface may take place because H protein masks the antibody recognition site on CD46 which results in "downregulation" of the expression of CD46.
Hu
Chunling
C
Key Laboratory of Virology, Ministry of Education, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, People's Republic of China.
Qi
Yipeng
Y
Zhang
Peng
P
Liu
Xin
X
Xu
Qin
Q
Chen
Xi
X
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D016466:CHO Cells; D002522:Chlorocebus aethiops; D003199:Computer Systems; D006224:Cricetinae; D017931:DNA Primers; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008458:Measles Vaccine; D008459:Measles virus / Q000235:genetics / Q000502:physiology*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014709:Vero Cells
D015703:Antigens, CD; C497857:CD46 protein, human; D017931:DNA Primers; D008458:Measles Vaccine; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D012367:RNA, Viral