title: Therapeutic antibody gene transfer: an active approach to passive immunity.
abstract: Advances in gene transfer approaches are enabling the possibility of applying therapeutic antibodies using DNA. In particular gene transfer in combination with electroporation is promising and can result in generating in vivo antibody concentrations in the low therapeutic range. However, several important problems need to be dealt with before antibody gene transfer can become a valuable supplement to the current therapies. As antibody production following gene transfer is difficult to control, the danger of inducing autoimmune conditions or uncontrollable side effects occurs in cases in which autologous antigens are targeted. It is suggested that the most promising area of application therefore appears to be infectious disease in which heterologous antigens are targeted and concerns for long-term antibody exposure are minimal. Finally, genes encoding fully human antibodies will enhance long-term expression and decrease problems linked to immunogenicity.
authors.affiliation: Genmab B.V., Yalelaan 60, P.O. Box 85199, 3508 AD Utrecht, The Netherlands.
authors.forename: Joost M
authors.initials: JM
authors.lastname: Bakker
authors.forename: Wim K
authors.initials: WK
authors.lastname: Bleeker
authors.forename: Paul W H I
authors.initials: PW
authors.lastname: Parren
mesh_terms: D000906:Antibodies / Q000235:genetics* / Q000276:immunology*; D015551:Autoimmunity; D003141:Communicable Diseases / Q000276:immunology / Q000628:therapy; D018274:Electroporation; D018014:Gene Transfer Techniques / Q000009:adverse effects; D005822:Genetic Vectors; D006801:Humans; D007116:Immunization, Passive
chemical_list: D000906:Antibodies