The CaPRP1 gene encoding a putative proline-rich glycoprotein is highly expressed in rapidly elongating early roots and leaves in hot pepper (Capsicum annuum L. cv. Pukang).
Most of the proline-rich cell wall glycoprotein genes isolated from higher plants are preferentially expressed in the transmitting tissues of the flower organ. In conducting expressed sequence tag (EST) analysis, which was prepared from 5-day-old early roots of hot pepper (Capsicum annuum L. cv. Pukang), we identified a cDNA clone, pCaPRP1, encoding a putative cell wall proline-rich glycoprotein. CaPRP1 (Mr=28 kDa, pI=9.98) was most closely related to Nicotiana alata NaPRP4 (71%), while most distantly related to soybean PvPRP (37%). The predicted primary structure of CaPRP1 contains a putative N-terminal signal peptide, six repeats of the Lys-Pro-Pro tripeptide, four repeats of a five-amino acid sequence [Pro-(Ser/The)-Pro-Pro-Pro] and one potential N-glycosylation site (Asn-Asn-Ser). In contrast to most proline-rich cell wall glycoprotein genes, CaPRP1 was highly expressed in rapidly elongating very early roots and young leaves as well as developing flower tissues. Although the physiological function of CaPRP1 is not yet clear, there are several possibilities for its role in cell expansion and elongation during early development of hot pepper plants.
Mang
Hyung Gon
HG
Department of Biology, College of Science, Yonsei University, Seoul 120-749, South Korea.
Lee
Jae-Hoon
JH
Park
Jong-A
JA
Pyee
Jaeho
J
Pai
Hyun-Sook
HS
Lee
JooHun
J
Kim
Woo Taek
WT
D000595:Amino Acid Sequence; D002212:Capsicum / Q000033:anatomy & histology / Q000502:physiology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism*; D055232:Proline-Rich Protein Domains; D012313:RNA / Q000378:metabolism; D016415:Sequence Alignment
D010455:Peptides; D010940:Plant Proteins; D012313:RNA