title: Neonatal exposure to diethylstilbestrol leads to impaired action of androgens in adult male hamsters.
abstract: Neonatal treatment with diethylstilbestrol (DES) leads to disruption of spermatogenesis in adult animals after apparently normal testicular development during puberty indicating aberrant androgen action in DES-exposed adult hamsters. The present study determined the effects of exogenous androgens in neonatally DES-exposed hamsters. Exogenous androgens failed to reverse the disruption of spermatogenesis in DES-exposed animals. Neonatal DES exposure caused a significant decrease in seminal vesicle weight, and abnormal histology. While exogenous androgens caused a significant increase in seminal vesicle weight in control animals, they failed to restore the seminal vesicle weight and normal histology in DES-exposed animals. Northern blot and/or RT-PCR analysis revealed that (1) AR, ERalpha and ERbeta mRNA levels were unchanged in DES-exposed animals, and (2) mRNA levels for the AR-responsive genes calreticulin, SEC-23B, and ornithine decarboxylase were significantly decreased in DES-exposed animals. Our results suggest that neonatal DES exposure impairs the action of androgens on target organs in male hamsters.
authors.affiliation: Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
authors.forename: SriTulasi
authors.initials: S
authors.lastname: Karri
authors.forename: Heather
authors.initials: H
authors.lastname: Johnson
authors.forename: William J
authors.initials: WJ
authors.lastname: Hendry
authors.forename: Simon C
authors.initials: SC
authors.lastname: Williams
authors.forename: Shafiq A
authors.initials: SA
authors.lastname: Khan
mesh_terms: D000726:Androgen Antagonists / Q000008:administration & dosage / Q000633:toxicity*; D000728:Androgens / Q000502:physiology / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D006224:Cricetinae; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000633:toxicity*; D013196:Dihydrotestosterone / Q000494:pharmacology; D004359:Drug Therapy, Combination; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D007279:Injections, Subcutaneous; D008297:Male; D009929:Organ Size / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000502:physiology / Q000633:toxicity*; D043343:Testosterone Propionate / Q000494:pharmacology
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D004968:Estrogens, Non-Steroidal; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D013196:Dihydrotestosterone; D013739:Testosterone; D004054:Diethylstilbestrol; D043343:Testosterone Propionate