Distribution of pH regulators in the rat laryngeal nerve: the spatial relationship between Na(+)/HCO(3)(-) cotransporters and Na(+)/H(+) exchanger type 3.
We studied the distribution of Na(+)/HCO(3)(-) cotransporters (NBC) and Na(+)/H(+) exchanger type 3 (NHE3) in the laryngeal nerve by immunohistochemistry to elucidate the spatial relationship of pH regulation system in the peripheral nerves. The nervous components, i.e., the nerve cells in the nodose and local ganglia and nerve fibers, were immunoreactive for NBC. Glial components such as Schwann cells and satellite cells surrounding nerve fibers and nerve cell bodies were also immunoreactive for NBC in most cases, while the cells comprising the perineurium and endoneurium were immunoreactive for NHE3. These results suggest that NBC-dependent pH regulation systems are present in the laryngeal nerve. Whereas, NHE3 may regulate extracellular pH rather than intracellular pH. In conclusion, spatial relationship of NBC and NHE3 in the laryngeal nerve would be important for pH regulation. Perineural lymph may have key role for acid-induced modulation of axons and Schwann cells.
Yamamoto
Yoshio
Y
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Morioka, Iwate 020-8550, Japan. yyoshio@iwate-u.ac.jp
Taniguchi
Kazuyuki
K
D000136:Acid-Base Equilibrium; D000818:Animals; D005455:Fluorescent Antibody Technique / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007823:Laryngeal Nerves / Q000378:metabolism*; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D027982:Sodium-Bicarbonate Symporters / Q000378:metabolism*; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
D016900:Neurofilament Proteins; C000613387:Slc9a3 protein, rat; D027982:Sodium-Bicarbonate Symporters; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; C051774:neurofilament protein H; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase