title: Stress regulation of sulfotransferases in male rat liver.
abstract: Sulfotransferase (SULT) catalyzed sulfation is responsible for hormone regulation and xenobiotic detoxification. Induction of SULTs by various hormones has been reported. Stress regulation of SULTs has not been reported, however. Here we report that rat liver SULTs can be regulated by physical stress (forced running, EX) and chemical stress (the organophosphorus pesticide parathion, PS). Both EX and PS increased rat liver phenol-sulfating SULT1A1 and hydroxysteroid-sulfating SULT2A1 activities. The increase in SULT1A1 activity did not correlate with protein (Western blot) or mRNA (RT-PCR) results but correlated well with increased non-protein soluble thiols. This suggests a possible Cys modification mechanism for stress regulation of SULT1A1. In vitro studies on GSH/GSSG effects on SULT1A1 activity support this conclusion. In contrast, SULT2A1 activity following physical or chemical stress treatments correlated well with protein and mRNA levels. This suggests a stress regulation mechanism of SULT2A1 at the gene transcription level, possibly occurring via hormones.
authors.affiliation: Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Smarajit
authors.initials: S
authors.lastname: Maiti
authors.forename: Shannon
authors.initials: S
authors.lastname: Grant
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Baker
authors.forename: Subramanya
authors.initials: S
authors.lastname: Karanth
authors.forename: Carey N
authors.initials: CN
authors.lastname: Pope
authors.forename: Guangping
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D015239:Arylsulfotransferase / Q000096:biosynthesis; D015153:Blotting, Western; D003600:Cytosol / Q000378:metabolism; D003720:Densitometry; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009284:Naphthols / Q000737:chemistry; D010805:Physical Conditioning, Animal; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D013438:Sulfhydryl Compounds / Q000737:chemistry; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000378:metabolism; D013455:Sulfur / Q000737:chemistry
chemical_list: D009284:Naphthols; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D012313:RNA; D013455:Sulfur; D015238:Sulfotransferases; D015239:Arylsulfotransferase; C117544:SULT1A1 protein, human; C028152:alcohol sulfotransferase; D005978:Glutathione; C028405:2-naphthol