title: Evidence for the presence of ferritin in plant mitochondria.
abstract: In this work, evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems and Arabidopsis thaliana cell cultures. The proteins were separated by SDS/PAGE. A protein, with an apparent molecular mass of approximately 25-26 kDa (corresponding to that of ferritin), was cross-reacted with an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunoprecipitation. The purified protein was analyzed by MALDI-TOF mass spectrometry and the results of both mass finger print and peptide fragmentation by post source decay assign the polypeptide sequence to the pea ferritin (P < 0.05). The mitochondrial localization of ferritin was also confirmed by immunocytochemistry experiments on isolated mitochondria and cross-sections of pea stem cells. The possible role of ferritin in oxidative stress of plant mitochondria is discussed.
authors.affiliation: Dipartimento di Biologia ed Economia Agro-Industriale, Sezione di Biologia Vegetale, Università di Udine, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Zancani
authors.forename: Carlo
authors.initials: C
authors.lastname: Peresson
authors.forename: Antonino
authors.initials: A
authors.lastname: Biroccio
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
authors.forename: Irene
authors.initials: I
authors.lastname: Murgia
authors.forename: Carlo
authors.initials: C
authors.lastname: Soave
authors.forename: Fulvio
authors.initials: F
authors.lastname: Micali
authors.forename: Angelo
authors.initials: A
authors.lastname: Vianello
authors.forename: Francesco
authors.initials: F
authors.lastname: Macrì
mesh_terms: D017360:Arabidopsis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000737:chemistry*; D008970:Molecular Weight; D018532:Pisum sativum / Q000737:chemistry; D059828:Plant Cells; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D011233:Precipitin Tests; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D005293:Ferritins