Electrophoretic analysis of di- and oligosaccharides derived from glycosaminoglycans on microchip format.
Microchip electrophoresis is a powerful tool for fast analysis of nucleic acids and has expanded its applicability to the analysis of various biological materials including proteins and carbohydrates. Glycosaminoglycans have intrinsic negative charges, and are good targets for electrophoretic analysis. In the present paper, we developed a method to analyze oligosaccharides and unsaturated disaccharides derived from some glycosaminoglycans after digestion with specific enzymes followed by derivatization with 2-aminoacrydone (AMAC) by reductive amination. The method described here allowed rapid analysis of oligosaccharides derived from glycosaminoglycans within 150 s with high sensitivity. We show an application of the present technique to the glycosaminoglycan analysis in cultured HeLa cells.
Matsuno
Yu-Ki
YK
Faculty of Pharmaceutical Sciences, Kinki University Kowakae 3-4-1, Higashi-osaka 577-8502, Japan.
Kinoshita
Mitsuhiro
M
Kakehi
Kazuaki
K
D000609:Aminoacridines / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000032:analysis; D004187:Disaccharides / Q000032:analysis; D046209:Electrophoresis, Microchip / Q000379:methods*; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis*; D012680:Sensitivity and Specificity
D000609:Aminoacridines; D004187:Disaccharides; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D009844:Oligosaccharides; C072515:2-aminoacridone; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates