title: Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection.
abstract: An original system which uses Porous Graphitic Carbon as support and a mixture of organic solvents as mobile phase is proposed for the analysis of triterpenic acids by liquid chromatography. The separation of betulinic acid, ursolic acid, oleanolic acid, and 18alpha- and 18beta-glycyrrhetinic acids was carried out within a short time and monitored by evaporative light scattering detection as universal detection method. Molecular modelling studies show that the main contribution to the selectivity comes from the electrostatic interaction characterised by the dipole moment of the products.
authors.affiliation: Institut de Chimie Organique et Analytique, UMR CNRS 6005, Université d'Orléans, BP 6759, F-45067 Orléans Cedex 2, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Bérangère
authors.forename: Nathalie
authors.initials: N
authors.lastname: Caussarieu
authors.forename: Philippe
authors.initials: P
authors.lastname: Morin
authors.forename: Luc
authors.initials: L
authors.lastname: Morin-Allory
authors.forename: Michel
authors.initials: M
authors.lastname: Lafosse
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002853:Chromatography, Liquid; D006034:Glycyrrhetinic Acid / Q000032:analysis; D008027:Light; D008956:Models, Chemical; D008958:Models, Molecular; D009828:Oleanolic Acid / Q000032:analysis; D053978:Pentacyclic Triterpenes; D012542:Scattering, Radiation; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis; D014466:Ultraviolet Rays; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D002244:Carbon; D006034:Glycyrrhetinic Acid; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid