Preventive effects of a traditional Chinese medicine (Sho-saiko-to) on endotoxin-induced cytotoxicity and tumor necrosis factor-alpha production in J774A.1 Cells.
Sho-saiko-to, one of the most frequently prescribed Kampo medicines, is used clinically to treat chronic hepatitis and has shown confirmed clinical efficacy. The present study investigated whether Sho-saiko-to can suppress cytotoxicity and tumor necrosis factor (TNF)-alpha production in endotoxin-treated J774A.1 cells. Sho-saiko-to (10-20 microg/ml) did not affect the proliferation of J774A.1 cells, while a high concentration (50 microg/ml) of Sho-saiko-to induced a slight reduction in cell viability. Treatment with Sho-saiko-to (10-50 microg/ml) significantly inhibited endotoxin (10 microg/ml)-induced cytotoxicity in J774A.1 cells. In addition, Sho-saiko-to (20 microg/ml) suppressed TNF-alpha production by endotoxin (1 microg/ml)-activated J774A.1 cells. These findings suggest that the Kampo prescription Sho-saiko-to suppresses cytotoxicity or TNF-alpha production in macrophages treated with endotoxin and that it may be useful in improving septic shock symptoms. Sho-saiko-to may therefore protect against some of the various disturbances caused by endotoxins through its ability to inhibit TNF-alpha production in septic shock.
Sakaguchi
Shuhei
S
First Department of Hygienic Chemistry; Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan. shuhei@tohoku-pharm.ac.jp
Furusawa
Shinobu
S
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004731:Endotoxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008516:Medicine, Chinese Traditional; D020835:Medicine, Kampo; D051379:Mice; D012486:Salmonella typhimurium; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D014409:Tumor Necrosis Factor-alpha; C461320:shosaiko-to