2D J-resolved spiral spectroscopic imaging at 7 T: application to mobile lipid mapping in a rat glioma.
Lactate (Lac) and mobile lipids (Lip), which are present in rat gliomas, are difficult to map because their 1H resonances overlap in the 1.3 ppm region. 2D J-resolved spectroscopy enables proper separation of the two resonance lines. To obtain high-spatial-resolution mapping of Lac and Lip resonances within a reasonable experiment time, we coupled 2D J-resolved spectroscopy with a fast spectroscopic imaging (SI) method, based on an out-and-in spiral k-space description. The method was applied to a rat glioma at 7 T, and Lac and Lip maps were reconstructed. The duration of SI (2D spatial, 2D spectral) was 64 min for a theoretical in-plane resolution of 1 x 1 mm, and a slice thickness of 2 mm (voxel size 8.2 microl, taking into account the point-spread function (PSF)).
Hiba
Bassem
B
Laboratoire Mixte INSERM-Université Joseph Fourier U594, Centre Hospitalier Universitaire, Grenoble, France.
Serduc
Raphaël
R
Provent
Peggy
P
Farion
Régine
R
Rémy
Chantal
C
Ziegler
Anne
A
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D005910:Glioma / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007773:Lactates / Q000378:metabolism; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
D007773:Lactates