Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia.
We report a case of IgG-kappa multiple myeloma associated with neutrophilia (WBC 31,300/microl, neutrophil 90.5%). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35% in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.
Kohmura
Kanoko
K
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Miyakawa
Yoshitaka
Y
Kameyama
Kaori
K
Kizaki
Masahiro
M
Ikeda
Yasuo
Y
D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis; D007964:Leukocytosis / Q000209:etiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D009101:Multiple Myeloma / Q000150:complications / Q000378:metabolism*; D009194:Myeloma Proteins / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism; D009504:Neutrophils; D011509:Proteoglycans / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053668:Syndecan-1; D053667:Syndecans
D007145:Immunoglobulin kappa-Chains; D008562:Membrane Glycoproteins; D009194:Myeloma Proteins; D009363:Neoplasm Proteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans; D016179:Granulocyte Colony-Stimulating Factor