[Induction of NO-synthase and glial fibrillary acidic protein in astrocytes of the temporal cortex in rats with audiogenic epileptiform reaction].
The localization of NADPH-diaphorase (NADPH-d), inducible NO-synthase (iNOS) and glial fibrillary acidic protein (GFAP) was studied in the astrocytes of the temporal cortex in rats of Krushinsky-Molodkina strain which are genetically prone to audiogenic seizures. The seizure was evoked by thrice-repeated acoustic stimulation. Wistar rats and acoustically untreated seizure-free Krushinsky-Molodkina rats were used as a control. The foci of brain damage were consistently found in the neocortex of the animals with audiogenic seizures. Epileptic foci, 300-400 microm in diameter, were localized in layers III-V; they were found to consist of the clusters of NADPH-d-positive astrocytes and to be present in both hemispheres. In the foci of cortical damage astrocytes expressed iNOS and an elevated level of GFAP. The number of GFAP-immunopositive astrocytes in the foci of damage was increased by 25-37% compared to the control and to undamaged areas of the cortex. Astrocyte NOS and GFAP induction found in this work, suggests the participation of glia in compensatory NO-dependent mechanisms, that are formed in the damage foci of neocortex during the audiogenic seizures.
Kalinichenko
S G
SG
Dudina
Iu V
IuV
Diuĭzen
I V
IV
Motavkin
P A
PA
D000161:Acoustic Stimulation; D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000378:metabolism*; D004195:Disease Models, Animal; D004790:Enzyme Induction; D020195:Epilepsy, Reflex / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D013045:Species Specificity; D013702:Temporal Lobe / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
D005904:Glial Fibrillary Acidic Protein; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D009252:NADPH Dehydrogenase