title: [Comparison of chromosomal defects in primary tumor and metastases by comparative genomic hybridization (CGH)].
abstract: Molecular methods can complement the classical methods in pathology like macroscopic, histological and immunohistochemical examinations.Comparative genomic hybridization is a cytogenetic method to screen for gains and losses of chromosomal material in tumor cells. This method allows defect studies of archival paraffin-embedded and formalin-fixed tumor material. CGH can detect gains and losses of chromosomal material that are at least 10 to 20 megabases in size. This genome-wide screening method allows to study the cytogenetic relationship between differently located tumors of a patient. To answer the question if these different tumors are metastases of the primary lung tumor or independent primary tumors CGH analysis is a supplementary method that introduces new prospectives.
authors.affiliation: Institut für Pathologie, Berufsgenossenschaftliche Kliniken Bergmannsheil Bochum. simon-franky@t-online.de
authors.forename: F
authors.initials: F
authors.lastname: Simon
authors.forename: M
authors.initials: M
authors.lastname: Vogt
authors.forename: C
authors.initials: C
authors.lastname: Kuhnen
authors.forename: G
authors.initials: G
authors.lastname: Johnen
authors.forename: K-M
authors.initials: KM
authors.lastname: Müller
mesh_terms: D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000235:genetics*; D002877:Chromosomes, Human; D006801:Humans; D009362:Neoplasm Metastasis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000469:parasitology*; D009693:Nucleic Acid Hybridization / Q000379:methods*