Analysis of hepatitis C virus quasispecies transmission and evolution in patients infected through blood transfusion.
OBJECTIVE
Studies on hepatitis C virus (HCV) quasispecies dynamics in the natural course of infection are rare owing to difficulties in obtaining samples from the early phase of infection.


METHODS
We studied 15 patients from the Transfusion-Transmitted Viruses Study who seroconverted to anti-HCV after receiving infected blood. Follow-up serum samples were collected every 2-3 weeks for 6 months, at 10 months, and at 11-16 years. Viral quasispecies in the second envelope hypervariable region 1 (E2/HVR1) and 5' untranslated region (5'UTR) were analyzed with single-strand conformation polymorphism (SSCP) and heteroduplex mobility assay (HMA).


RESULTS
Seven patients cleared infection within 7-24 weeks (mean, 14.0 wk) and 3 patients eventually became anti-HCV negative. In 6 patients with resolving hepatitis the SSCP band pattern remained stable, whereas in one patient minor changes appeared before clearance. In contrast, in all 8 patients progressing to chronicity, major changes in the E2/HVR1 quasispecies developed at 8-22 weeks (mean, 13.1 wk). Shannon entropy and medium mobility shift values derived from HMA gels remained stable in patients with resolving hepatitis but changed in those who developed chronic infection. Only 2 patients showed minor changes in 5'UTR. A decrease in E2/HVR1 complexity at the time of transmission (bottleneck) was found in 5 patients altogether.


CONCLUSIONS
Changes in E2/HVR1 quasispecies 8-22 weeks after infection, likely caused by mounting immune pressure, were predictive of ensuing chronic infection, whereas stability was associated with resolution. Our study also showed that composition of HCV quasispecies may be preserved during transmission from host to host.
Laskus
Tomasz
T
Department of Medicine, Mayo Clinic Scottsdale, Scottsdale, Arizona, USA.
Wilkinson
Jeffrey
J
Gallegos-Orozco
Juan F
JF
Radkowski
Marek
M
Adair
Debra M
DM
Nowicki
Marek
M
Operskalski
Eva
E
Buskell
Zelma
Z
Seeff
Leonard B
LB
Vargas
Hugo
H
Rakela
Jorge
J
D000328:Adult; D018562:Disease Transmission, Infectious; D019143:Evolution, Molecular; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000635:transmission / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013045:Species Specificity; D065227:Transfusion Reaction