title: [Expression and significance of estrogen receptor isoforms in the development of human breast carcinoma].
abstract: OBJECTIVE
  To investigate the estrogen receptors (ER)alpha and ERbeta expression and their relationship with clinicopathological parameters in human breast carcinoma.
  
  
  METHODS
  Samples were obtained from 30 breast carcinoma, reverse transcriptase polymerase chain reaction was used to measure the expression of ERalpha and ERbeta mRNA.
  
  
  RESULTS
  ERalpha mRNA level was up-regulated in breast carcinoma tissue compared with adjacent normal tissue (t = 7.399, P < 0.01) while down-regulated in ERbeta. The relative ratio of ERalpha and ERbeta was decreased in normal tissue vs. carcinoma (t = 6.385, P < 0.01), in patients with lymph node metastasis vs. those without lymph node metastasis (t = 2.602, P < 0.05), in late stage carcinoma vs. early stage (t = 3.754, P < 0.05).
  
  
  CONCLUSIONS
  ERalpha and ERbeta play divergent role in the development of human breast carcinoma.
authors.affiliation: Breast Disease Center, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Jia-qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Qiao
authors.forename: De-qi
authors.initials: DQ
authors.lastname: Yang
authors.forename: Fu-zhong
authors.initials: FZ
authors.lastname: Tong
authors.forename: Ying-jiang
authors.initials: YJ
authors.lastname: Ye
authors.forename: Zhi-rong
authors.initials: ZR
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D047629:Estrogen Receptor beta / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger