Influence of cultivating conditions on the alpha-galactosidase biosynthesis from a novel strain of Penicillium sp. in solid-state fermentation.
OBJECTIVE
The work is intended to achieve optimum culture conditions of alpha-galactosidase production by a mutant strain Penicillium sp. in solid-state fermentation (SSF).


RESULTS
Certain fermentation parameters involving incubation temperature, moisture content, initial pH value, inoculum and load size of medium, and incubation time were investigated separately. The optimal temperature and moisture level for alpha-galactosidase biosynthesis was found to be 30 degrees C and 50%, respectively. The range of pH 5.5-6.5 was favourable. About 40-50 g of medium in 250-ml flask and inoculum over 1.0 x 10(6) spores were suitable for enzyme production. Seventy-five hours of incubation was enough for maximum alpha-galactosidase production. Substrate as wheat bran supplemented with soyabean meal and beet pulp markedly improved the enzyme yield in trays.


CONCLUSIONS
Under optimum culture conditions, the alpha-galactosidase activity from Penicillium sp. MAFIC-6 indicated 185.2 U g(-1) in tray of SSF.


CONCLUSIONS
The process on alpha-galactosidase production in laboratory scale may have a potentiality of scaling-up.
Wang
C L
CL
National Feed Engineering and Technology Research Centre, China Agricultural University, Beijing, China.
Li
D F
DF
Lu
W Q
WQ
Wang
Y H
YH
Lai
C H
CH
D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D013696:Temperature; D014867:Water; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000235:genetics*
D003470:Culture Media; D014867:Water; D000519:alpha-Galactosidase