title: [Expression of Bcl-2 mRNA and Bcl-2 protein in nasal mucusa of rat in allergic rhinitis model].
abstract: OBJECTIVE
  To study the expression of Bcl-2 (B-cell lymphoma/leukemia-2) mRNA and Bcl-2 protein in nasal mucusa of rat in allergic rhinitis (AR) model.
  
  
  METHODS
  Twenty male or female SD rats, weighing 180-220 g, free of nasal diseases, were randomly divided into two groups. AR model was established through intraperitoneal injection of ovabumin (OVA) as well as nasal challenge with OVA. The nasal mucusa thus obtained from two groups were studied by HE, in situ hybridization histochemical staining of Bcl-2 mRNA and immunohistochemical staining of bcl-2/bax protein.
  
  
  RESULTS
  The positive expression of Bcl-2 mRNA and Bcl-2/bax protein was presented in the glandular cells,epithelium cells and eosinophils. The staining density of Bcl-2 mRNA and Bcl-2 protein in AR rats increased significantly as compared with those in normal controls. No significant differences of Bax protein expression in two groups were found.
  
  
  CONCLUSIONS
  Bcl-2 mRNA and Bcl-2 protein were up-regulated in the mucusa of AR rats.
authors.affiliation: Department of Otolaryngology, the Second Hospital of Shanxi Medical University, Taiyuan, 030001, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Suo
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhao
authors.forename: Yunfang
authors.initials: Y
authors.lastname: An
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhengde
authors.initials: Z
authors.lastname: Tao
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics* / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger