Analysis of altered protein expression patterns of Chlamydia pneumoniae by an integrated proteome-works system.
We have identified, analyzed, and quantified differential protein expression profile of five C. pneumoniae proteins, Adk (adenylate kinase), AhpC (thiol-specific antioxidant), CrpA (15 KD cysteine rich protein), Map (methionine aminopeptidae), and Cpn0710 (hypothetical protein) under normal versus persistent growth conditions induced by interferon-gamma, at different time intervals of their replicative cycle by successfully employing the latest proteomic analysis tool, PDQuest 2-D analysis software. We have also determined that this software represents a reliable analytical tool for mapping protein expression patterns in C. pneumoniae.
Mukhopadhyay
Sanghamitra
S
Division of Infectious Diseases, University of Louisville, Louisville, Kentucky 40292, USA.
Miller
Richard D
RD
Summersgill
James T
JT
D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D016993:Chlamydophila pneumoniae / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics
D001426:Bacterial Proteins; D020543:Proteome; D007371:Interferon-gamma