Nitric oxide spatial distribution in single cultured hippocampus neurons: investigation by projection of reconstructed 3-D image and visualization technique.
Recent studies have revealed a non-homogeneous distribution of nitric oxide synthase (NOS) in neurons. However, it is not yet clear whether the intracellular distribution of NOS represents the intracellular nitric oxide (NO) distribution. In the present study, software developed in our laboratory was applied to the reconstructed image obtained from confocal slice images in order to project the 3-D reconstructed images in any direction and to cut the neuron in different sections. This enabled the spatial distribution of NO to be visualized in any direction and section. In single neurons, NO distribution was seen to be heterogeneous. After stimulation with glutamate, the spatial changes in different areas of the neuron were different. These findings are consistent with immunocytochemical data on the intracellular localization of nNOS in hippocampus neurons, and will help to elucidate the specificity of nitric oxide signaling. Finally, the administration of SNAP and L-NAME was used to examine DAF-2 distribution in the neurons. The results showed this distribution to be homogenous; therefore, it did not account for the NO distribution results.
Yang
Yong
Y
Department of Biomedical Engineering, Zhejiang University (Yuqun Campus), Hangzhou 310027, PR China.
Ning
Gang-Min
GM
Kutor
John
J
Hong
Di-Hui
DH
Zhang
Mu
M
Zheng
Xiao-Xiang
XX
D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D009569:Nitric Oxide / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology
D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine