title: Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens.
abstract: Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.
authors.affiliation: Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire de Lille, France. n-lemaitre@chru-lille.fr
authors.forename: Nadine
authors.initials: N
authors.lastname: Lemaître
authors.forename: Sylvie
authors.initials: S
authors.lastname: Armand
authors.forename: Anne
authors.initials: A
authors.lastname: Vachée
authors.forename: Odile
authors.initials: O
authors.lastname: Capilliez
authors.forename: Christine
authors.initials: C
authors.lastname: Dumoulin
authors.forename: René J
authors.initials: RJ
authors.lastname: Courcol
mesh_terms: D006801:Humans; D008168:Lung / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*