[Anti-SARS virus activities of different recombinant human interferons in cell culture system].
BACKGROUND
To study the anti-SARS virus activities of different recombinant human interferons on the cell culture system.


METHODS
Anti-SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda) cell culture.


RESULTS
The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5+/-129.5) IU/ml, (149.0+/-71.7) IU/ml, (69.5+/-61.5) IU/ml, (87.3+/-47.1) IU/ml, respectively or (0.6+/-0.5) ng/ml, (10.6+/-5.1) ng/ml, (3.5+/-3.1) ng/ml, (0.9+/-0.5) ng/ml, respectively.


CONCLUSIONS
All the tested recombinant interferons showed anti-SARS virus activities on the Rda cell culture with different sensitivities.
Duan
Zhao-jun
ZJ
Beijing Jin Di Ke Biotechnology Institute, State Research Center for Biotechnological Engineering, Beijing 100176, China.
Zhang
Li-lan
LL
Xie
Zhi-ping
ZP
Yu
Zhi-ai
ZA
Zhang
Li-ping
LP
Zhang
Bin
B
Liu
Yong-qing
YQ
Wang
Jian-wei
JW
Li
Wu-ping
WP
Zhang
Cheng-hai
CH
Ma
Xue-jun
XJ
Shu
Yue-long
YL
Duan
Shu-min
SM
Li
De-xin
DX
Hou
Yun-de
YD
D000998:Antiviral Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D011994:Recombinant Proteins; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects*; D045169:Severe Acute Respiratory Syndrome / Q000188:drug therapy / Q000821:virology
D000998:Antiviral Agents; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins