[Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants].
The encoding sequence of gus gene from Escherichia coli was fused with maize Ubi-1 promoter and was introduced into maize genome via particle bombardment. Fertile transgenic maize plants were regenerated from bombarded type-I calluses which were derived from scutellar tissue of immature embryos based on PPT selection. Expression activity of gus gene under the control of Ubi-1 promoter was analysed using histochemical method, and the results showed that gus gene expressed in most tissues except anther. Ubi-GUS expression in pollen, egg cell and T1 immature embryos revealed that this promoter was active in early stages of plant development. Histochemically stained pollen grains of T0 plants showed a 1:1 segregation of the gus gene, which suggested that the foreign gene was inherited in Mendelian model in these plants. In addition, maize Ubi-1 promoter could reduce the copy number of foreign genes in transgenic maize plants, which might be useful in avoidance of gene silencing. T1 seeds have been harvested.
Xu
Zi Qin
ZQ
Provincial Key Laboratory of Biotechnology, Northwest University, Xian 710069.
Jia
Jing Fen
JF
Hao
Jian Guo
JG
Wang
Ying Juan
YJ
Feng
Shu Zhen
SZ
D015152:Blotting, Northern; D015139:Blotting, Southern; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D025822:Ubiquitin C / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D010940:Plant Proteins; C417884:Ubi-1 protein, Zea mays; D025822:Ubiquitin C