title: Effect of c-fos overexpression on development and proliferation of peritoneal B cells.
abstract: We examined effects of c-fos overexpression on the development and property of peritoneal B-1 cells using transgenic (H2-c-fos) mice carrying the c-fos gene under the control of the constitutive H-2Kb promoter. The number of B-1b cells in the peritoneal cavity of H2-c-fos mice was 4-fold larger than that in control littermates. Although the numbers of total peritoneal B cells and B-1a cells were similar between them, the peritoneal B-2 cell number in H2-c-fos mice was reduced to 50% of control littermates, suggesting the effect of c-fos overexpression on a balance of B-1b and B-2 cells in a peritoneal cavity. Adoptive transfer experiments with hematopoietic stem cells of H2-c-fos and control mice into irradiated H2-c-fos mice demonstrated that the augmentation of B-1b cells is due to the c-fos effect in B cells and the effect on environment of the peritoneal cavity of H2-c-fos mice. When peritoneal B cells were cultured with LPS in the presence or absence of IL-4, cell proliferation of B-1b cells was the highest among these peritoneal B cell subsets, and the proliferation of H2-c-fos B-1b cells was 3-fold higher than that of control B-1b cells. This augmentation is due to the c-fos effect in B cells. IgG1 production of B-1b cells in these cultures was slightly higher than those of B-1a and peritoneal B-2 cells. Thus, the c-fos overexpression augments development of B-1b cells in a peritoneal cavity and proliferation of peritoneal B-1b cells to LPS.
authors.affiliation: Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mori
authors.forename: Akemi
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
authors.forename: Lisa
authors.initials: L
authors.lastname: Fujimura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Arima
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hatano
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokuhisa
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D005434:Flow Cytometry; D016762:Genes, fos / Q000235:genetics / Q000276:immunology*; D007107:Immune System / Q000254:growth & development*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D010529:Peritoneal Cavity / Q000502:physiology
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides