title: Life-history traits of standard and autochthonous cladocerans: II. Acute and chronic effects of acetylsalicylic acid metabolites.
abstract: Metabolic products are often more toxic than their pharmacological parent compounds. Therefore, the acute and chronic effects of the main metabolites--salicylic acid (SAL), gentisic acid (GEN), and o-hydroxyhippuric acid (HDP)--of acetylsalicylic acid (ASA), the active ingredient in Aspirin and many other pharmaceuticals, were assessed using standard (Daphnia magna) and autochthonous (Daphnia longispina) cladocerans. The sequence of decreasing levels of acute and chronic toxicity of ASA metabolites to daphnids was GEN > SAL > HDP. HDP did not present acute toxicity, but chronic exposures enabled the production of abnormal neonates and, in particular, egg abortion. Thus, reproduction was the end point most susceptible to HDP. On the other hand, SAL and GEN induced changes in the normal patterns of reproduction and growth of both species. In general, D. longispina was more sensitive than was D. magna, although the population growth of the autochthonous species was superior under SAL exposures than that of the standard test species. Although the concentrations that were determined to have a toxic effect were above the levels detected in aquatic environmental samples, exposure to low levels of pharmacologically active substances for a duration longer than the test period may induce changes in nontarget organisms.
authors.affiliation: Universidade de Aveiro, Departamento de Biologia, 3810-193 Aveiro, Portugal. catarina@bio.ua.pt
authors.forename: Catarina R
authors.initials: CR
authors.lastname: Marques
authors.forename: Nelson
authors.initials: N
authors.lastname: Abrantes
authors.forename: Fernando
authors.initials: F
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D001241:Aspirin / Q000378:metabolism; D033361:Cladocera / Q000502:physiology*; D003621:Daphnia / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D005618:Fresh Water; D005841:Gentisates / Q000633:toxicity*; D006626:Hippurates / Q000633:toxicity*; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D011174:Portugal; D012098:Reproduction / Q000187:drug effects; D020156:Salicylic Acid / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D005841:Gentisates; D006626:Hippurates; D014874:Water Pollutants, Chemical; C000693:salicylurate; D020156:Salicylic Acid; D001241:Aspirin; C010925:2,5-dihydroxybenzoic acid