title: Proton conductance of influenza virus M2 protein in planar lipid bilayers.
abstract: Purified M2 protein from the Udorn strain of influenza virus was reconstituted into planar lipid bilayers from liposomes. In 1 mM HCl, the single-channel conductance was measured as 6 pS with open probability of < or =0.03. The current voltage curve is linear over the achievable voltage range. The current amplitude is amantadine sensitive. In HCl solutions, the single-channel current was essentially invariant with changes in [Cl(-)], [Na(+)], and [tetraethylammonium] ([TEA(+)]), but dependent on [H(+)]. The reversal potential, determined with asymmetrical hydrogen chloride solution, is very close to the equilibrium potential of hydrogen. This appears to be the first report of single-channel proton currents with the full-length M2 protein.
authors.affiliation: Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah 84602, USA.
authors.forename: Viksita
authors.initials: V
authors.lastname: Vijayvergiya
authors.forename: Ryan
authors.initials: R
authors.lastname: Wilson
authors.forename: Adam
authors.initials: A
authors.lastname: Chorak
authors.forename: Philip Fei
authors.initials: PF
authors.lastname: Gao
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Cross
authors.forename: David D
authors.initials: DD
authors.lastname: Busath
mesh_terms: D000547:Amantadine / Q000737:chemistry / Q000494:pharmacology; D004553:Electric Conductivity; D004594:Electrophysiology; D006851:Hydrochloric Acid / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D007477:Ions; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000378:metabolism; D008564:Membrane Potentials; D011522:Protons; D012964:Sodium / Q000737:chemistry; D019789:Tetraethylammonium / Q000737:chemistry; D014763:Viral Matrix Proteins / Q000737:chemistry*; D000086082:Viroporin Proteins
chemical_list: D000547:Amantadine; D006851:Hydrochloric Acid; D006859:Hydrogen; D007477:Ions; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D011522:Protons; D012964:Sodium; D019789:Tetraethylammonium; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins