Transcriptional pattern analysis of adrenergic immunoregulation in mice. Twelve hours norepinephrine treatment alters the expression of a set of genes involved in monocyte activation and leukocyte trafficking.
We investigated in vivo effects of norepinephrine (NE) on the transcription of 200 immunologically relevant genes in the mouse. Balb/c mice were s.c. implanted with NE containing retard tablets. Twelve hours later, splenic mRNA was prepared and hybridized onto cDNA microarrays containing the sequences of the major cytokines, their receptors and all CD-antigens of the mouse. Consistent results were obtained with a set of five genes: in the NE-treated animals four genes (CXCR4, VCAM1, IL-1R2, CD 14) were found 2-8 fold upregulated as compared to sham treated animals, whereas the gene for CCR3 was downregulated (< 0.5 fold). The findings were confirmed using quantitative reverse transcriptase Real Time PCR. These first results prove the usefulness of gene microarray technology towards transcription pattern analysis in neuroimmune interactions. Furthermore, they support the relevance of catecholamines in the regulation of leukocyte migration and the inflammatory response.
Gruber-Olipitz
M
M
Institute of Pathophysiology, Medical University of Graz, Heinrichstrasse 31, A-8010 Graz, Austria.
Stevenson
R
R
Olipitz
W
W
Wagner
E
E
Gesslbauer
B
B
Kungl
A
A
Schauenstein
K
K
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology*; D015536:Down-Regulation / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D009490:Neurosecretory Systems / Q000187:drug effects / Q000276:immunology*; D009638:Norepinephrine / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D054397:Receptors, CCR3; D019718:Receptors, CXCR4 / Q000235:genetics; D019707:Receptors, Chemokine / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000235:genetics; D053574:Receptors, Interleukin-1 Type II; D013312:Stress, Physiological / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
C515351:Ccr3 protein, mouse; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D054397:Receptors, CCR3; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine; D017472:Receptors, Interleukin-1; D053574:Receptors, Interleukin-1 Type II; D019010:Vascular Cell Adhesion Molecule-1; D009638:Norepinephrine