Non-ideal tracer sedimentation equilibrium: a powerful tool for the characterization of macromolecular interactions in crowded solutions.
Non-ideal tracer sedimentation equilibrium is a technique devised to quantify the effect of high concentrations of unrelated macromolecules on the self- or hetero-associations of dilute macromolecules. Principles and experimental techniques are reviewed, and previous experimental work summarized. A new analysis of experimental data is presented that requires no a priori assumptions regarding the nature of weak repulsive interactions between solute species and the concentrated (crowding) species.
Rivas
Germán
G
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain.
Minton
Allen P
AP
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D005340:Fibrinogen / Q000032:analysis / Q000737:chemistry; D046911:Macromolecular Substances / Q000032:analysis / Q000737:chemistry*; D008962:Models, Theoretical; D011849:Radioactive Tracers; D012996:Solutions
D046911:Macromolecular Substances; D011849:Radioactive Tracers; D012996:Solutions; D005340:Fibrinogen