title: The effect of xylanase on lignocellulosic components during the bleaching of wood pulps.
abstract: HPLC, SEM and XRD techniques have been proposed as methods for ascertaining the changes occurring in polysaccharides (cellulose and xylans) and fibres during the xylanase bleaching processes. TCF and ECF bleached pulps with and without enzyme pretreatment were analysed. The ratio of carbohydrates present in the pulp, observation of changes occurring in the surface of the fibres and the crystallinity and accessibility of the bleached fibres were determinated. These characteristics have been related with pulp properties. Xylan content decreased when pulp was bleached. Xylanase treatment substantially reduced the xylose content present in pulp, measured by HPLC after the hydrolysis method of the sample. Morphological changes in the fibres occurred when the enzymatic treatment was applied. Bleaching increased the crystallinity of the pulp and enzyme pretreatment also affected the crystallinity of cellulose fibres
authors.affiliation: Department of Textile and Paper Engineering, E.T.S.E.I.T. Polytechnical University of Catalonia. Colom 11, 08222 Terrassa, Spain. roncero@etp.upc.es
authors.forename: M Blanca
authors.initials: MB
authors.lastname: Roncero
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Torres
authors.forename: José F
authors.initials: JF
authors.lastname: Colom
authors.forename: Teresa
authors.initials: T
authors.lastname: Vidal
mesh_terms: D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005052:Eucalyptus / Q000648:ultrastructure; D007221:Industry / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010209:Paper; D014934:Wood; D014961:X-Ray Diffraction
chemical_list: C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases