Degradation of phenanthrene by Trametes versicolor and its laccase.
Phenanthrene is a three-ring polycyclic aromatic hydrocarbon and commonly found as a pollutant in various environments. Degradation of phenanthrene by white rot fungus Trametes versicolor 951022 and its laccase, isolated in Korea, was investigated. After 36 h of incubation, about 46% and 65% of 100 mg/l of phenanthrene added in shaken and static fungal cultures were removed, respectively. Phenanthrene degradation was maximal at pH 6 and the optimal temperature for phenanthrene removal was 30 degrees C. Although the removal percentage of phenanthrene was highest (76.7%) at 10 mg/l of phenanthrene concentration, the transformation rate was maximal (0.82 mg/h) at 100 mg/L of phenanthrene concentration in the fungal culture. When the purified laccase of T versicolor 951022 reacted with phenanthrene, phenanthrene was not transformed. The addition of redox mediator, 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) or 1-hydroxybenzotriazole (HBT) to the reaction mixture increased oxidation of phenanthrene by laccase about 40% and 30%, respectively.
Han
Mun-Jung
MJ
Division of Biological Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Choi
Hyoung-Tae
HT
Song
Hong-Gyu
HG
D052160:Benzothiazoles; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010616:Phenanthrenes / Q000378:metabolism*; D020072:Polyporales / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013451:Sulfonic Acids / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014230:Triazoles / Q000378:metabolism
D052160:Benzothiazoles; D003470:Culture Media; D010616:Phenanthrenes; D013451:Sulfonic Acids; D014230:Triazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C031181:phenanthrene; C011852:1-hydroxybenzotriazole; D042845:Laccase