Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS.
A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.
Casabianca
Anna
A
Institute di Biological Chemistry Giorgio Fornaini, University of Urbino, Italy.
Orlandi
Chiara
C
Fraternale
Alessandra
A
Magnani
Mauro
M
D000818:Animals; D019380:Anti-HIV Agents / Q000627:therapeutic use; D052160:Benzothiazoles; D003959:Diamines; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000821:virology*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000254:growth & development* / Q000302:isolation & purification; D011804:Quinolines; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity; D019562:Viral Load
D019380:Anti-HIV Agents; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D012337:RNA, Ribosomal, 18S; D012367:RNA, Viral; C098022:SYBR Green I