Use of serial analysis of gene expression (SAGE) technology reveals new granulocytic markers.
Because of its short life span in blood, its low RNA content and its highly condensed nucleus, the granulocyte was initially considered as a terminally differentiated cell unable to express novel genes. However, mature granulocytes still contain a variety of mRNAs and may respond to external stimuli by rapid and complex changes in gene expression. The present work was undertaken to provide a wider view of the gene expression profile in unstimulated circulating PMNs. We used serial analysis of gene expression (SAGE) adapted to downsized extracts (SADE) to cope with their small mRNA content. As cell samples of the highest degree of purity were crucial for this project, we adapted a magnetic cell sorting method to reach the required high levels of purity (99.55 +/- 0.19%) together with low activation rates (1.37 +/- 0.28). We analyzed 20,787 tags identifying 8547 different transcripts, of which 47.8% were unknown, 34.6% were transcripts of known genes, and 13.8% matched with expressed sequence tags (EST). Highly expressed genes were those involved in cell mobility, diapedesis, cell signaling and destruction of micro-organisms. In addition, this method led to the identification of genes which had not previously been reported in granulocytes. These results could provide new molecular markers and a reliable reference for the investigation of pathologies involving alteration of the granulocyte gene expression profile.
Bertrand
Gérald
G
Etablissement Français du Sang Pyrénées-Méditerranée, Laboratoire R&D Agents Transmissibles par Transfusion, 240 Avenue Emile Jeanbrau, 34094 Montpellier cedex 5, France.
Coste
Joliette
J
Segarra
Christiane
C
Schved
Jean-François
JF
Commes
Thérèse
T
Marti
Jacques
J
D015415:Biomarkers; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D012333:RNA, Messenger