[Effects of anti- CXCR4 monoclonal antibody on adhesiveness of human acute myelocytic leukemia cell line HL-60 and expression of Bcl-2, Fas proteins].
To study the importance of chemokine SDF-1 in surviving of acute myelocytic leukemia cells HL-60, the adhesion ability of HL-60 and expression of Bcl-2, Fas protein when SDF-1 activity was blocked by anti-CXCR4 monoclonal antibody (12G5) were compared with those detected before MAb incubation, in this experiment, HL-60 cell were cultured and co-cultured with normal marrow stromal cell. The adhesion rate was detected while the expression of Bcl-2 and Fas proteins were assayed by immunohistochemical technique when SDF-1 activity was inhibited. The results showed that cell adhesion rate of HL-60 decreased while the expression Bcl-2 decreased and Fas increased. It is concluded that inhibition of SDF-1 activity increases cell apoptosis and thus reduces life-span of leukemia cell at certain level.
Wei
Li
L
Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Kong
Pei-Yan
PY
Chen
Xing-Hua
XH
Peng
Xian-Gui
XG
Chang
Cheng
C
Zeng
Dong-Feng
DF
Liu
Hong
H
Liu
Lin
L
Wang
Qing-Yu
QY
Zhang
Yi
Y
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis; D002448:Cell Adhesion; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000502:physiology*; D018922:HL-60 Cells / Q000737:chemistry / Q000166:cytology; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D019718:Receptors, CXCR4 / Q000502:physiology*; D019014:fas Receptor / Q000032:analysis*
D000911:Antibodies, Monoclonal; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019253:Proto-Oncogene Proteins c-bcl-2; D019718:Receptors, CXCR4; D019014:fas Receptor