Recombinant bivalent vaccine against foot-and-mouth disease virus serotype O/A infection in guinea pig.
In this study, two DNA fragments encoding amino acid (141-160)-(21-140)-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.
Yi
Jian-Zhong
JZ
State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai, China.
Liu
Ming-Qiu
MQ
Zhu
Cai-Zhu
CZ
Zhang
Qiang
Q
Sheng
Zu-Tian
ZT
Du
Qing-Yun
QY
Yan
Wei-Yao
WY
Zheng
Zhao-Xin
ZX
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000939:Epitopes; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology*; D006168:Guinea Pigs; D012703:Serotyping; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines