title: Silica sol-gel/organic hybrid material for protein encapsulated column of capillary electrochromatography.
abstract: A new-type of sol-gel/organic hybrid composite material using gelatin or chitosan with tetramethoxysilane was developed for the bovine serum albumin (BSA)-encapsulated monolithic column for capillary electrochromatography (CEC). The composite monolith was used to immobilize BSA in a fused-silica capillary. The addition of gelatin and chitosan to the alkoxysilane enabled the enantioseparation of Trp. A very small amount of these polymers were effective for the enantioseparation. Especially, the monolithic column prepared from chitosan with tetramethoxysilane showed a high enantioselectivity for Trp enantiomers and the value (alpha' = t2/t1, t1: fast eluted enantiomer, t2: second eluted enantiomer) reached 1.15 on CEC mode. Furthermore, the composite materials exhibited a higher stability compared to the silica sol-gel column. These results showed that the sol-gel/organic hybrid composite was useful as a monolithic matrix for the BSA-encapsulated column for CEC.
authors.affiliation: Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada Shizuoka, Shizuoka 422-8526, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kato
authors.forename: Hazuki
authors.initials: H
authors.lastname: Saruwatari
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai-Kato
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Toyo'oka
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D005782:Gels; D009930:Organic Chemicals / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D005782:Gels; D009930:Organic Chemicals; D011506:Proteins; D012822:Silicon Dioxide