title: Plumbagin quantification in roots of Plumbago scandens L. obtained by different extraction techniques.
abstract: The Plumbago genus belongs to the Plumbaginaceae family and it is known due to its variety of biological uses, most of them attributed to the presence of naphthoquinones. Plumbagin is a naturally occurring naphthoquinone that can be obtained from roots of Plumbago scandens L. In order to find out the better technique for plumbagin extraction, were applied: static maceration, dynamic maceration, with assistance of ultrasonic waves and in Soxhlet apparatus. Four compounds were qualitatively detected in all extracts: the naphthoquinones plumbagin and epi-isoshinanolone, palmitic acid and sitosterol. Plumbagin was always the major component in all analyzed extracts and it was quantitatively determined by gas chromatograph coupled with mass spectrometer. Soxhlet was the most efficient extraction technique however, prolonged heating time promoted plumbagin degradation.
authors.affiliation: Laboratório de Química de Produtos Naturais, Fiocruz, Rio de Janeiro, RJ, 21041-250, Brasil. s_paiva@far.fiocruz.br
authors.forename: Selma R
authors.initials: SR
authors.lastname: de Paiva
authors.forename: Lucilene A
authors.initials: LA
authors.lastname: Lima
authors.forename: Maria Raquel
authors.initials: MR
authors.lastname: Figueiredo
authors.forename: Maria Auxiliadora C
authors.initials: MA
authors.lastname: Kaplan
mesh_terms: D002849:Chromatography, Gas; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D029606:Plumbaginaceae / Q000737:chemistry*
chemical_list: D009285:Naphthoquinones; D010936:Plant Extracts; C014758:plumbagin