Allogenic antibody-mediated identification of head and neck cancer antigens.
Recently, we described a new target-identification technology, autoantibody-mediated identification of antigens (AMIDA). AMIDA takes advantage of autologous serum autoantibodies to identify disease-associated antigens. Here, we evaluated the allogenic variant of AMIDA (allo-AMIDA), using permanent cancer cell lines as an antigen-pool rather than primary biopsy samples. Twelve different proteins were retrieved exclusively with antibodies from cancer patients, but not from healthy donors. The expression of three of these antigens, e-FABP, hnRNP H, and Grb2, was evaluated in more detail. All three proteins were strongly overexpressed in primary carcinomas and metastases thereof, as compared to healthy epithelium. Additionally, serum reactivity against e-FABP was detected in 20% of cancer patients but only 2% of healthy volunteers. In summary, we demonstrate that permanent cancer cell lines represent a reliable source for tumour-associated antigens. Moreover, we show that allo-AMIDA is suitable for the identification of tumour-specific antigens overcoming the limitations of autologous screening techniques.
Rauch
Jens
J
Department of Head and Neck Surgery, Ludwig-Maximilians-Universität München, Marchioninistr.15, D-81377 Munich, Germany.
Ahlemann
Martin
M
Schaffrik
Martina
M
Mack
Brigitte
B
Ertongur
Suna
S
Andratschke
Michaela
M
Zeidler
Reinhard
R
Lang
Stephan
S
Gires
Olivier
O
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000737:chemistry*; D001323:Autoantibodies / Q000737:chemistry; D002352:Carrier Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D051380:GRB2 Adaptor Protein; D006258:Head and Neck Neoplasms / Q000276:immunology* / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D007159:Immunologic Tests; D047468:Immunoprecipitation; D009362:Neoplasm Metastasis; D011994:Recombinant Proteins / Q000737:chemistry
D048868:Adaptor Proteins, Signal Transducing; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D002352:Carrier Proteins; C493412:FABP5 protein, human; D050556:Fatty Acid-Binding Proteins; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D011994:Recombinant Proteins