[Calcium ions localization in mixed synapses of mauthner cells after exposure to substances changing the gap junction conductivity].
Using the pyroantimonate method, the distribution of calcium ions in Mauthner cell mixed synapses was studied following their treatment with substances changing gap junction (GJ) synapse electrotonic conductivity. Ecdyson, the drug known to increase GJ conductivity, induced the appearance of calcium pyroantimonate precipitates covering the whole postsynaptic area of GJs, making them highly asymmetric. Formation of precipitate grains was also observed in the cleft of desmosome-like contacts (DLCs). Chlorpromazine, substance that decreases GJ conductivity, induced the formation of precipitates in GJ cleft and on both pre- and postsynaptic sides of their surfaces. No precipitates were seen in DLC cleft. These results show that ecdyson acts as a substance selectively increasing the GJ conductivity with no effect on DLC function. Chlorpromazine has dual effect, by blocking the communication through both GJ and DLC. Thus, using chemicals that change GJ permeability, the possibility of their interaction with actin-containing structures which also participate in the electrotonic signal transfer, should be taken into consideration.
Pavlik
L L
LL
Bezgina
E N
EN
Dzeban
D A
DA
Moshkov
D A
DA
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002413:Cations, Divalent; D002746:Chlorpromazine / Q000494:pharmacology; D004441:Ecdysterone / Q000494:pharmacology; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006054:Goldfish / Q000502:physiology*; D009431:Neural Conduction / Q000187:drug effects* / Q000502:physiology; D009475:Neurons, Afferent / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D013569:Synapses / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure
D002413:Cations, Divalent; D004441:Ecdysterone; D002118:Calcium; D002746:Chlorpromazine