Biodiversity amongst cultivable polycyclic aromatic hydrocarbon-transforming bacteria isolated from an abandoned industrial site.
The characterisation of a microbial community of a polycyclic aromatic hydrocarbons (PAHs) contaminated site (formerly Carbochimica, Trento, Italy) was carried out. A preliminary evaluation of the heterogeneity and the metabolic activity of the microbial community were attempted by denaturing gradient gel electrophoresis (DGGE) and reverse transcriptase-denaturing gel electrophoresis (RT-DGGE). The presence of a heterogeneous and metabolically active microbial community was found. To evaluate the PAH-transforming potential of the soil bacterial community, enrichment cultures were set up. Taxonomically diverse bacteria, showing different biochemical PAH-transforming pathways were obtained. Some of the isolates showed not nah-homologous PAH-transforming genotypes.
Zocca
Chiara
C
Laboratories of Microbial Biotechnology and Environmental Microbiology, Department of Science and Technology, University of Verona, Strada Le Grazie 15 - Ca' Vignal, 37134 Verona, Italy.
Di Gregorio
Simona
S
Visentini
Filippo
F
Vallini
Giovanni
G
D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants