[Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV].
OBJECTIVE
To investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV.


METHODS
HIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits.


RESULTS
All 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different.


CONCLUSIONS
The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.
Song
Ai-jing
AJ
Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Li
Xiu-hua
XH
Li
Juan
J
Xu
Si-hong
SH
Zhang
Feng
F
Wan
Yan-min
YM
Zhang
Chun-tao
CT
Wang
You-chun
YC
D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006678:HIV / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood*; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic