In vivo clearance of surfactant lipids during acute pulmonary inflammation.
BACKGROUND
A decrease in pulmonary surfactant has been suggested to contribute to the lung dysfunction associated with pulmonary inflammation. A number of studies have implicated surfactant clearance as a possible mechanism for altered pool sizes. The objective of the current study was to specifically investigate the mechanisms of surfactant clearance in a rodent model of acute pulmonary inflammation.


METHODS
Inflammation was induced by intrapulmonary instillation of lipopolysaccharide (LPS: 100 microg/kg). Lipid clearance was assessed at 18 and 72 hours post-LPS instillation by intratracheal administration of radiolabel surfactant-like liposomes 2 hours prior to isolation and analysis of inflammatory cells and type II cells.


RESULTS
At both 18 and 72 hours after LPS instillation there was significantly less radioactivity recovered in the lavage fluid compared to respective control groups (p < 0.05). At both time points, the number of cells recovered by lavage and their associated radioactivity was greater compared to control groups (p < 0.01). There was no difference in recovery of radioactivity by isolated type II cells or other cells obtained from enzymatic digestion of lung tissue.


CONCLUSIONS
These results show that increased clearance of surfactant lipids in our model of acute pulmonary inflammation is primarily due to the inflammatory cells recruited to the airspace and not increased uptake by alveolar type II cells.
Malloy
Jaret L
JL
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. j.malloy@cellbio.duke.edu <j.malloy@cellbio.duke.edu>
Wright
Jo Rae
JR
D000208:Acute Disease; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D050356:Lipid Metabolism; D008070:Lipopolysaccharides; D008297:Male; D008657:Metabolic Clearance Rate; D011014:Pneumonia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D011663:Pulmonary Surfactants