Metabolomic analysis of molecular species of phospholipids from normotensive and preeclamptic human placenta electrospray ionization mass spectrometry.
Electrospray ionization mass spectrometry (ESI/MS) is a highly sensitive, fast and powerful technique for the metabolite and metabolomic analysis of mixtures of lipids in a biological extract. We have exploited this technique to identify and characterize various phospholipids present in the placenta of preeclamptic and normal pregnant women. Multiple major molecular species can be detected in each phospholipid class have arachidonic acid as major fatty acid constituent. There is no remarkable difference in the molecular composition in each of these phospholipids in both the extracts. However, there seems to be lower amounts of the plasmenyl phosphatidylethanolamine and greater amounts of free fatty acids in preeclamptic placenta.
Jain
Shashi
S
Department of Neurology, University of Cincinnati, Cincinnati OH 45267-0536, USA.
Jayasimhulu
Koka
K
Clark
Joseph F
JF
D001095:Arachidonic Acids / Q000737:chemistry; D002845:Chromatography; D002855:Chromatography, Thin Layer; D019856:Ethanolamine / Q000737:chemistry; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D006801:Humans; D007477:Ions; D013058:Mass Spectrometry; D010743:Phospholipids / Q000737:chemistry; D010920:Placenta / Q000378:metabolism*; D010955:Plasmalogens / Q000737:chemistry; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001095:Arachidonic Acids; D005230:Fatty Acids, Nonesterified; D007477:Ions; D010743:Phospholipids; D010955:Plasmalogens; D019856:Ethanolamine