Quantitative determination of albendazole metabolites in sheep spermatozoa and seminal plasma by liquid chromatographic analysis with fluorescence detection.
A new analytical method for the simultaneous quantitative determination of albendazole metabolites in sheep spermatozoa and seminal plasma at levels down to 46.5 ng/mL for albendazole sulphoxide (ABZ-SO), 7.5 ng/mL for albendazole sulphone (ABZ-SO2) and 12 ng/mL for albendazole 2-aminosulphone (ABZ-SO2NH2) has been developed. Analytes were extracted from alkalinized samples with ethyl acetate. Separation was carried out on a C18 column in the presence of tetra-n-butylammonium (TBA) hydrogen sulphate and octanesulphonate sodium (OCT), as ion-pair agents. Fluorometric detection was performed with excitation and emission wavelengths set at 290 and 320 nm, respectively. Accuracy data showed overall recoveries (+/-S.E.M.) of 83.1+/-1.2% for ABZ-SO, 98.8+/-0.6% for ABZ-SO2 and 85.3+/-0.7% for ABZ-SO2NH2, in spermatozoa. Respective values in seminal plasma were 88.0+/-0.9%, 97.7+/-0.5% and 93.2+/-1.7%. Precision data suggested coefficient of variation (CV%) values lower than 5.9% for spermatozoa and 3.8% for seminal plasma. The method was successfully applied for the determination of the three albendazole metabolites in semen samples collected from rams that had been orally administered albendazole.
Batzias
G C
GC
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Aristotle University of Thessaloniki, GR-54 124 Thessaloniki, Greece. batzias@vet.auth.gr
Theodosiadou
E
E
Delis
G A
GA
D015766:Albendazole / Q000031:analogs & derivatives* / Q000032:analysis*; D000818:Animals; D000871:Anthelmintics / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D004356:Drug Storage; D005615:Freezing; D008297:Male; D015394:Molecular Structure; D012661:Semen / Q000737:chemistry*; D012756:Sheep; D013094:Spermatozoa / Q000737:chemistry*
D000871:Anthelmintics; C027187:albendazole sulfone; C085926:albendazole-2-aminosulfone; D015766:Albendazole; C027186:albendazole sulfoxide