[Repopulation with exogenous mitochondria in the cell].
To create human mitochondrial DNA (mtDNA)-transferred cells as cell model of mitochondria defects-related diseases, platelet of normal young and old subjects were isolated as donor of mtDNA, then fused with mtDNA-depleted cells (rho0 cells) under induction of PEG1500. Auxtrophy test, cytochrome c oxidase activity assay and PCR amplification of mtDNA were done to confirm the transferring of mtDNA. Cell clones were visible in the medium 10 to 15 days after fusion, which grew well in medium lacking uridine, had positive COX activity and contained objective fragment of mtDNA by PCR, opposite to rho0 cells. Transferring of mtDNA to rho0 cells was identified, and mtDNA-transferred cell models were successfully created.
Liu
Fang
F
Department of Pharmacology, Xuan-Wu Hospital of Capital University of Medical Sciences, Beijing Key Laboratory for Brain Aging, Beijing 100053.
Zhang
Lan
L
Li
Lin
L
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology; D002460:Cell Line; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000502:physiology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV