title: Multiplex PCR method for identifying recombinant vaccine-related polioviruses.
abstract: The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.
authors.affiliation: Respiratory and Enteric Virus Branch, G-10, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov
authors.forename: David R
authors.initials: DR
authors.lastname: Kilpatrick
authors.forename: Karen
authors.initials: K
authors.lastname: Ching
authors.forename: Jane
authors.initials: J
authors.lastname: Iber
authors.forename: Ray
authors.initials: R
authors.lastname: Campagnoli
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Freeman
authors.forename: Nada
authors.initials: N
authors.lastname: Mishrik
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Liu
authors.forename: Mark A
authors.initials: MA
authors.lastname: Pallansch
authors.forename: Olen M
authors.initials: OM
authors.lastname: Kew
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification*; D023321:Poliovirus Vaccines / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods*; D014614:Vaccines, Synthetic / Q000592:standards*
chemical_list: D017931:DNA Primers; D023321:Poliovirus Vaccines; D014614:Vaccines, Synthetic