title: Role of interconnections in porous bioceramics on bone recolonization in vitro and in vivo.
abstract: The interconnections in a porous biomaterial are the pathways between the pores. They conduct cells and vessels between pores. Thus they favour bone ingrowth inside ceramics. The aim of our study was to determine the effect on bone ingrowth of interconnections in two ceramics: hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) with the same porosity of about 50% and a mean pores size of 100-300 microm and a mean interconnection size of 30-100 microm. In vitro, four discs for osteoblast culture were studied after 14 and 28 days of incubation. The results show that human osteoblasts can penetrate interconnections over 20 microm in size, and colonize and proliferate inside macropores, but the most favourable size is over 40 microm. In vivo, eight cylinders were implanted in the middle shaft of both rabbit femurs for 12 or 24 weeks. The histomorphometric results show that interconnections in porous ceramics favour bone ingrowth inside the macropores. In the HA group the rate of calcification and bone ingrowth do not differ, and chondroid tissue is observed inside pores. But in beta-TCP, the calcification rate and the bone ingrowth increased significantly. At week 12 significant correlation between new bone ingrowth and the size of the interconnections is observed between new bone ingrowth and the density of pores. In conclusion we notice that in vivo a 20 microm interconnection size only allows cell penetration and chondroid tissue formation; however the size of the interconnections must be over 50 microm to favour new bone ingrowth inside the pores. We propose the concept of "interconnection density" which expresses the quantity of links between pores of porous materials. It assures cell proliferation and differentiation with blood circulation and extracellular liquid exchange. In resorbable materials, pore density and interconnection density are more important than their size, contrary to unresorbable materials in which the sizes and the densities are equally important.
authors.affiliation: Institut de Recherche sur les Maladies du Squelette, Ru de Docteur Calot, 62608 Berck-Sur-Mer Cedex, France.
authors.forename: J X
authors.initials: JX
authors.lastname: Lu
authors.forename: B
authors.initials: B
authors.lastname: Flautre
authors.forename: K
authors.initials: K
authors.lastname: Anselme
authors.forename: P
authors.initials: P
authors.lastname: Hardouin
authors.forename: A
authors.initials: A
authors.lastname: Gallur
authors.forename: M
authors.initials: M
authors.lastname: Descamps
authors.forename: B
authors.initials: B
authors.lastname: Thierry