Production of bacterial cellulose by Acetobacter xylinum BPR2001 using molasses medium in a jar fermentor.
Bacterial cellulose (BC) production by Acetobacter xylinum subsp. sucrofermentans BPR2001 using molasses medium was carried out in a jar fermentor. When molasses was subjected to H(2)SO(4)-heat treatment, the maximum BC concentration increased to 76% more than that achieved using untreated molasses, and the specific growth rate increased 2-fold. When the initial sugar concentrations in the H(2)SO(4)-heat treated molasses were varied from 23 g/l to 72 g/l, BC concentration, production rate, and yield were maximum at sugar concentrations of 23 g/l and 37 g/l, and production of by-products, such as polysaccharides and CO(2), was lower than at sugar concentrations of 48 g/l and 72 g/l, indicating that maintaining a lower molasses concentration is essential for efficient BC production in jar fermentors, this being due mainly to the complex nature of molasses. Molasses has a clear advantage over pure sugars as a carbon source from an economic viewpoint.
Bae
S O
SO
Chemical Resources Laboratory, Tokyo Institute of Technology, R1-29-4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
Shoda
M
M
D019149:Bioreactors; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D008965:Molasses; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013464:Sulfuric Acids
D003470:Culture Media; D011135:Polysaccharides, Bacterial; D013464:Sulfuric Acids; D002245:Carbon Dioxide; D002482:Cellulose; C033158:sulfuric acid