title: Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene.
abstract: Rhodococcus sp. strain T104 is able to utilize both limonene and biphenyl as growth substrates. Furthermore, T104 possesses separate pathways for the degradation of limonene and biphenyl. Previously, we found that a gene(s) involved in limonene degradation was also related to indigo-producing ability. To further corroborate this observation, we have cloned and sequenced a 8,842-bp genomic DNA region with four open reading frames, including one for indole oxygenase, which converts indole to indigo (a blue pigment), The reverse transcription PCR data demonstrated that the identified indole oxygenase gene is specifically induced by limonene, thereby implicating this gene in the degradation of limonene by T104.
authors.affiliation: Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Choi
authors.forename: Dockyu
authors.initials: D
authors.lastname: Kim
authors.forename: Sung-Cheol
authors.initials: SC
authors.lastname: Koh
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: So
authors.forename: Jong-Sul
authors.initials: JS
authors.lastname: Kim
authors.forename: Eungbin
authors.initials: E
authors.lastname: Kim
mesh_terms: D003001:Cloning, Molecular; D053138:Cyclohexenes; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D000077222:Limonene; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010105:Oxygenases / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013729:Terpenes / Q000378:metabolism*
chemical_list: D053138:Cyclohexenes; D004269:DNA, Bacterial; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013729:Terpenes; D000077222:Limonene; D010105:Oxygenases; D049308:Dioxygenases