The cme gene of Clostridium difficile confers multidrug resistance in Enterococcus faecalis.
Antibiotic resistance in C. difficile by efflux has been previously suggested. The genome of C. difficile 630 was screened for sequences encoding putative proteins homologous to NorA from Staphylococcus aureus. Four ORFs homologous to efflux genes were cloned into the pAT79 shuttle vector under the control of transcription and translation signals of Gram-positive bacteria and expressed in Enterococcus faecalis JH2-2 and S. aureus RN4220. One of these sequences, designated cme conferred resistance to ethidium bromide, safranin O, and erythromycin in E. faecalis. The three other ORFs did not confer detectable resistance in both bacteria.
Lebel
Sacha
S
Faculté de Pharmacie, Département de Microbiologie, Unité Ecosystème Microbien Digestif et Santé, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France. sacha.lebel@cep.u-psud.fr
Bouttier
Sylvie
S
Lambert
Thierry
T
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active; D003001:Cloning, Molecular; D016360:Clostridioides difficile / Q000235:genetics*; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016366:Open Reading Frames; D010619:Phenazines / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004917:Erythromycin; D004996:Ethidium; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010619:Phenazines; D011994:Recombinant Proteins; C075040:NorA protein, Staphylococcus; C009195:safranine T