title: [Correlation of expression of RhoA (RhoC and their effector ROCK-1 with malignant phenotype of ovarian cancer cells in vitro].
abstract: OBJECTIVE
  To investigate the expression of RhoA, RhoC and their effector ROCK-1 in four ovarian cancer cell lines in vitro and their correlation with invasiveness.
  
  
  METHODS
  Expression of RhoA, RhoC and ROCK-1 mRNA and protein in four ovarian cancer cell lines SW626, Skov-3, A2780 and Caov-3 was detected by RT-PCR and Western blot assay. Invasion assay was done in Boyden chamber.
  
  
  RESULTS
  The expression levels of RhoA, RhoC and ROCK-1 mRNA and protein varied in the four different cell lines examined. The expression level of RhoC, but not RhoA and ROCK-1, was significantly correlated with the invasive capability of these cells in vitro (r = 0.95, P < 0.01). Expression of RhoA at the level of transcription was not correlated with that at the translation level. The expression of RhoA and RhoC did not correlate with that of ROCK-1.
  
  
  CONCLUSIONS
  Expression level of RhoC may serve as an independent parameter in evaluating metastasis and become a new target in inhibiting ovarian cancer metastasis.
authors.affiliation: Department of Obstetrics & Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Han
authors.forename: A-li
authors.initials: AL
authors.lastname: Zhang
authors.forename: Ming-fu
authors.initials: MF
authors.lastname: Wu
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Fu-jun
authors.initials: FJ
authors.lastname: Li
authors.forename: Qing-lei
authors.initials: QL
authors.lastname: Gao
authors.forename: Guo-ning
authors.initials: GN
authors.lastname: Liao
authors.forename: Yun-ping
authors.initials: YP
authors.lastname: Lu
authors.forename: Shi-xuan
authors.initials: SX
authors.lastname: Wang
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D020741:rho GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D000075024:rhoC GTP-Binding Protein
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C066737:RHOA protein, human; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D000075024:rhoC GTP-Binding Protein