title: [Baculovirus expression of two human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus].
abstract: OBJECTIVE
  To develop human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus (HAV) by baculovirus expression system.
  
  
  METHODS
  The heavy and light chain genes of two human-derived neutralizing Fab antibodies to HAV were cloned into baculovirus expression vector Pac-kappa-Fc and Pac-L-Fc, and further expressed in insect cells as IgG antibodies. The IgG products were purified and well characterized.
  
  
  RESULTS
  The baculovirus expressed McAb HAFc16 fully retained the specificity of binding to hepatitis A virus and the competition with mouse anti-hepatitis A virus McAb using ELISA. The viral neutralization assay in vitro demonstrated the retention of antibody function after expression of the human antibody in insect cells. The other expressed antibody HAFc78 also has the neutralizing activity but it is directed against different epitopes of HAV when compared with HAFc16.
  
  
  CONCLUSIONS
  The recombinant baculovirus/insect cells expressed human neutralizing IgG antibodies to hepatitis A virus retained all biological functions specific for hepatitis A virus. The results provided the possibility of using these antibodies to rapidly protect high risk or early exposure populations from hepatitis A virus infection.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Cao
authors.forename: Mi-fang
authors.initials: MF
authors.lastname: Liang
authors.forename: Qing-ling
authors.initials: QL
authors.lastname: Meng
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Wang
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Xu
authors.forename: Ke-qian
authors.initials: KQ
authors.lastname: Guo
authors.forename: Mei-yun
authors.initials: MY
authors.lastname: Zhan
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
authors.forename: De-xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D030041:Hepatitis A virus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006508:Hepatitis Antibodies; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D011994:Recombinant Proteins