Amplification of inhibitory activity of catechin against disease-related enzymes by conjugation on poly(epsilon-lysine).
A new inhibitor against disease-related enzymes, collagenase, hyaluronidase, and xanthine oxidase, has been developed by the laccase-catalyzed conjugation of catechin on poly(epsilon-lysine). The resulting poly(epsilon-lysine)-catechin conjugate showed greatly improved inhibition effects on activity of these enzymes, whereas the catechin monomer showed very low inhibition activity. The kinetic analysis on the inhibition of collagenase exhibited that the conjugate was a mixed-type inhibitor. The amplified activities might offer high potential as a therapeutic agent for prevention of various enzyme-related diseases.
Ihara
Noriko
N
Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, 615-8510 Japan.
Schmitz
Sarah
S
Kurisawa
Motoichi
M
Chung
Joo Eun
JE
Uyama
Hiroshi
H
Kobayashi
Shiro
S
D002392:Catechin / Q000737:chemistry* / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011107:Polylysine; D002392:Catechin