MGluRs regulate the expression of neuronal calcium sensor proteins NCS-1 and VILIP-1 and the immediate early gene arg3.1/arc in the hippocampus in vivo.
The metabotropic glutamate receptor (mGluR) agonist (R,S)-3,5-dihydroxyphenylglycine (DHPG) is involved in several forms of hippocampal synaptic plasticity. DHPG application can induce slow-onset potentiation, a form of long-term potentiation (LTP), in the dentate gyrus and in the CA1 region in vivo. The induction of LTP correlates with increased expression levels of neuronal calcium sensor (NCS), considered as key elements for plasticity. In this study we investigated mGluR- and time-dependent changes in the expression of two different NCS proteins. Following DHPG application in vivo NCS-1 and VILIP-1 expression increased, with significant levels reached after 8 and 24h. The effect was attenuated by treatment with the group I mGluR specific antagonist S-4-carboxyphenylglycine. The immediate early gene (IEG) arg3.1/arc showed highest expression levels 2h after DHPG-treatment. Therefore, mGluRs at concentrations which induce synaptic plasticity regulate the expression of IEGs and NCS proteins in different time frames and thus contribute to late phases of synaptic plasticity.
Brackmann
Marian
M
Signal Transduction Research Group, Neuroscience Research Center of the Charite, Tucholskystrasse 2, D-10117 Berlin, Germany.
Zhao
Congjian
C
Kuhl
Dietmar
D
Manahan-Vaughan
Denise
D
Braunewell
Karl-Heinz
KH
D000818:Animals; D020013:Calcium Signaling / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology; D003598:Cytoskeletal Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D017781:Genes, Immediate-Early / Q000187:drug effects / Q000235:genetics*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000235:genetics*; D007276:Injections, Intraventricular; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051596:Neurocalcin; D051580:Neuronal Calcium-Sensor Proteins; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000235:genetics*; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology
D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; D017874:Immediate-Early Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D044169:Receptors, Calcium-Sensing; D018094:Receptors, Metabotropic Glutamate; C496690:Vsnl1 protein, rat; C092069:activity regulated cytoskeletal-associated protein; C453101:carboxyphenylglycine; C101464:frequenin calcium sensor proteins; D008734:Methoxyhydroxyphenylglycol; D005998:Glycine; C010117:3,4-dihydroxyphenylglycol