Bisphenol A exerts thyroid-hormone-like effects on mouse oligodendrocyte precursor cells.
We report studies on the mechanism of action of bisphenol A (BPA) on the differentiation of oligodendrocyte precursor cells (OPCs). Our results show that: (1) BPA inhibits the differentiation of OPCs induced by exposure to thyroid hormone (T3). (2) The effect is mediated through various mechanisms via the thyroid hormone receptor (TRbeta1) which is considered to be responsible for OPC differentiation. (3) The action of BPA on OPC differentiation does not involve the FcRgamma-Fyn-myelin basic protein (MBP) cascade as an inducer of OPC differentiation nor does it suppress CREB phosphorylation, which is considered to be induced by the T3-TR complex. (4) The presence of MBP isoforms (21.5, 18.5, 17.0 and 14.0 kDa) was detected in OPCs, and the expression of exon 2-containing isoforms (i.e. 17.0 and 21.5 kDa) was upregulated upon treatment with T3. In contrast, expression of MBP was inhibited by BPA.
Seiwa
Chika
C
Glial Cell Research Group, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Nakahara
Jin
J
Komiyama
Takatsugu
T
Katsu
Yoshinao
Y
Iguchi
Taisen
T
Asou
Hiroaki
H
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001559:Benzhydryl Compounds; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D004676:Myelin Basic Protein / Q000187:drug effects / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects*; D037042:Thyroid Hormone Receptors beta / Q000096:biosynthesis; D014284:Triiodothyronine / Q000378:metabolism; D000098826:Bisphenol A Compounds
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D004676:Myelin Basic Protein; D010636:Phenols; D020033:Protein Isoforms; D037042:Thyroid Hormone Receptors beta; D014284:Triiodothyronine; C006780:bisphenol A; D000098826:Bisphenol A Compounds