Isolation and characterization of an IAA-responsive gene from Gossypium barbadense L.
The full-length cDNA of an IAA-responsive gene was cloned from Gossypium barbadense L. (designated as Gbiaa-Re) by rapid amplification of cDNA ends (RACE). Gbiaa-Re gene was 1043-bp long and contained a 573-bp open reading frame encoding a polypeptide of 190 amino acid residues. Homology analysis revealed that Gbiaa-Re strongly resembled known plant IAA-responsive genes. The conserved integrated domain "AUX_IAA, AUX/IAA family" resided within the region from L11, to V190 of GbIAA-RE, and the 4 typically conserved domains of IAA-responsive gene family were all found in GbIAA-RE. The secondary structure of GbIAA-RE consisted of 20.53% alpha helix, 13.68% extended strand and 65.79% random coil. In total, 12 phosphorylation sites, 1 N-glycosylation site and 4 O-beta-GlcNAc attachment sites were predicted. Southern blot analysis indicated that Gbiaa-Re belonged to a low-copy gene family. Semi-quantitative PCR analysis indicated that the expression of Gbiaa-Re gene was inducible by IAA. Our studies suggested that Gbiaa-Re was a new member of plant AUX/IAA gene family.
Song
Jun
J
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Chai
Yourong
Y
Pang
Yongzhen
Y
Zuo
Kaijing
K
Fei
Jiong
J
Liu
Xuefeng
X
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000187:drug effects*; D003368:Gossypium / Q000235:genetics*; D019807:Iodoacetic Acid / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018387:Codon, Initiator; D018388:Codon, Terminator; D017931:DNA Primers; D018076:DNA, Complementary; D019807:Iodoacetic Acid