Characterization of the rutin-metal complex by electrospray ionization tandem mass spectrometry.
According to the strong application background of bioflavonoid and metal-flavonoid complexes, novel electrospray ionization tandem mass spectrometry (ESI-MSn) was applied to investigate the structure and fragmentation mechanism of transition metal-rutin complexes. In the full-scan mass spectra, different stoichiometric ratios of rutin-metal complexes were found. In the reaction between rutin and Cu, four kinds of complexes with four different stoichiometric ratios were produced. In the reaction between rutin and Zn, Mn(II), and Fe(II), only two kind of complexes with stoichiometric ratios of 1:1 and 1:2 occured. In further tandem mass spectrometric experiments of different rutin-metal complexes, product fragments came from the neutral loss of the external rhamnose and the internal glucose unit, oligosaccharide chain, aglycone, and small organic molecules. According to the MSn data, we proposed a mechanism for all fragments of the rutin-Cu complex A and the structure of two rutin-Cu complexes, C and D.
Bai
Yu
Y
Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P R, China.
Song
Fengrui
F
Chen
Maolian
M
Xing
Junpeng
J
Liu
Zhiqiang
Z
Liu
Shuying
S
D003300:Copper / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000032:analysis* / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D015032:Zinc / Q000737:chemistry
D008670:Metals; D009942:Organometallic Compounds; D008345:Manganese; D012431:Rutin; D003300:Copper; D007501:Iron; D015032:Zinc