Genetic diversity (RAPD-PCR) of lactobacilli isolated from "Almagro" eggplant fermentations from two seasons.
Genetic diversity of 323 strains of lactobacilli isolated from an Almagro eggplant manufacturing plant was analyzed by using random amplified polymorphic DNA (RAPD). Thirty-four distinct RAPD patterns were obtained with 95% of isolates grouped into 18 main clusters. Genetic diversity was higher in brines from season II, Lactobacillus plantarum and Lactobacillus fermentum/cellobiosus being the species with the greatest number of genotypes. A single L. fermentum/cellobiosus genotype comprised isolates from both seasons and could be considered endemic to that factory.
Seseña
Susana
S
Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias del Medio Ambiente, Avda. Carlos III s/n, 45071 Toledo, Spain. ssprieto@amb-to.uclm.es
Sánchez
Isabel
I
Palop
Llanos
L
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005285:Fermentation; D005511:Food Handling; D005516:Food Microbiology; D014644:Genetic Variation; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D032323:Solanum melongena / Q000382:microbiology*
D004269:DNA, Bacterial