[Evaluation of isolation media for the detection of Bartonella henselae--isolation of Bartonella henselae from domestic cats].
Bartonella henselae is a causative agent of cat scratch disease. We preliminarily tested four media for the bacterial growth, including agar plates with sheep, horse or rabbit blood, and chocolate agar. Of these media, rabbit blood and chocolate agar plate were found to be more excellent for the growth than the medium with sheep or horse blood. Blood samples from 60 domestic cats in Yamaguchi Prefecture were then cultured using 7% rabbit blood agar plates and BACTEC9050 (BD), automated blood culture microbial detection system. B. henselae was isolated from six of the 60 (10%) blood samples. Tiny colonies of B. henselae were visible on the agar medium after one week of culture at 35 degrees C in the 5% CO2 atmosphere. BACTEC 9050 detected B. henselae in one of the 10 blood samples and it took two weeks to detect the bacteria automatically, though gram stain failed to show organisms in the blood culture bottle. In conclusion, rabbit blood or chocolate agar and incubation of agar media more than one week and of BACTEC more than two weeks are recommended for the detection of B. henselae.
Tsuneoka
Hidehiro
H
Department of Clinical Laboratory, Yamaguchi-Kouseiren Nagato General Hospital.
Ishida
Chizuru
C
Umeda
Akiko
A
Inokuma
Hisashi
H
Tsukahara
Masato
M
D000362:Agar; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D018416:Bartonella henselae / Q000254:growth & development / Q000302:isolation & purification*; D002372:Cat-Scratch Disease / Q000382:microbiology; D002415:Cats / Q000382:microbiology*; D003470:Culture Media / Q000592:standards*; D005069:Evaluation Studies as Topic; D006736:Horses; D011817:Rabbits; D012756:Sheep
D003470:Culture Media; D000362:Agar