title: [Interspecies nuclear transfer using nonquiescent somatic cell].
abstract: It is the point at issue in intraspecies nuclear transfer whether quiescence is necessary for development of nuclear transfer reconstructed embryos. In the interspecies nuclear transfer, some reports have proved that quiescent cell is able to support preimplantation development of the interspecies reconstructed embryos. Are non-quiescent cells able to support preimplantation development of the interspecies reconstructed embryos? We used non-quiescent somatic cells from C57BL/6 mice and giant pandas as donors to transfer into enucleated rabbit oocytes. After electrofusion (the electrofusion rates were 62.2% and 71.6%, respectively) and electrical activation, 5.1% of those mouse-rabbit reconstructed embryos developed to blastocyst in vitro, and 4.2% of panda-rabbit reconstructed embryos developed to blastocyst after transferring into ligated rabbit oviduct. These results indicate that non-quiescent cell from C57BL/6 mouse and giant panda could be dedifferentiated in enucleated rabbit oocytes and support early embryo development.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080.
authors.forename: Jin Song
authors.initials: JS
authors.lastname: Li
authors.forename: Zhi Ming
authors.initials: ZM
authors.lastname: Han
authors.forename: Zhi Yu
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Duan Cheng
authors.initials: DC
authors.lastname: Wen
authors.forename: Zhong Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Min Kang
authors.initials: MK
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Lian
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.forename: Peng Yan
authors.initials: PY
authors.lastname: Wang
authors.forename: He Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Da Yuan
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D011817:Rabbits; D001503:Ursidae