title: Identification of two new HLA alleles: B*3546* and B*5611*. How reliable are the published HLA-B intron 2 sequences?
abstract: Polymerase chain reaction-sequence-specific primer (PCR-SSP) typing for human leukocyte antigen (HLA)-B in a male 25-year-old Caucasian individual of Iranian origin and in a 42-year-old German Caucasian bone marrow donor revealed reaction patterns that did not agree with any known HLA specificity, thus suggesting in both cases the existence of a novel allele. Sequence-based typing (SBT) after allelic separation revealed the sequences of the new alleles HLA-B*5611 and B*3546. The sequence patterns of both new alleles might have been generated as the results of double crossing over, possibly over several generations. During the analysis of the HLA-B*3546 intron 2 sequence for possible crossing over points, a base insert, an additional G after position 700, was found. This insert was analyzed using SBT and PCR-SSP and was found to be present not only in all samples carrying B*35, but also in all HLA-B specificities tested. It appears that all known HLA-B alleles may contain a G insert at position 700 of intron 2, and that the published intron 2 sequence alignments of the HLA-B locus may contain errors at this position.
authors.affiliation: Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Heidelberg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Czachurski
authors.forename: S
authors.initials: S
authors.lastname: Scherer
authors.forename: S
authors.initials: S
authors.lastname: Gehrke
authors.forename: G
authors.initials: G
authors.lastname: Laux
authors.forename: G
authors.initials: G
authors.lastname: Opelz
authors.forename: J
authors.initials: J
authors.lastname: Mytilineos
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005091:Exons / Q000235:genetics*; D005858:Germany; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D015235:HLA-B Antigens