title: Effect of sodium nitroprusside on ischemia-reperfusion injuries of the rat liver.
abstract: OBJECTIVE
  The role of NO (nitric oxide) in liver ischemia-reperfusion injury remains controversial, therefore the effect of sodium nitroprusside (SNP), as NO donor on ischemia-reperfusion injuries of the rat liver was studied.
  
  
  METHODS
  Warm ischemia (68% of total liver) of the rat liver was induced for 30 min, followed by reperfusion for 60 min. A dose of 1 or 10 microg/kg/min SNP were continuously administered during the reperfusion period. We performed the following assessments: (i) hepatic microcirculation, (ii) nitrosyl-hemoglobin (Hb-NO, a product of interactions between hemoglobin and NO) levels, (iii) NO2-/NO3- (end products of NO oxidation) levels, and (iv) the serum lactate-dehydrogenase (LDH) levels.
  
  
  RESULTS
  Hepatic microcirculation at 5 and 15 min after start of SNP infusion was significantly improved, compared with that in the control group. NO2-/NO3- and Hb-NO levels in both SNP groups were gradually elevated after reperfusion. Serum LDH levels at 60 min after start of reperfusion in both SNP groups were significantly lower than in the control group.
  
  
  CONCLUSIONS
  In the early phase of the reperfusion period, SNP injected increased hepatic microcirculation and reduced hepatocyte damage, suggesting that the administration of SNP may help present ischemia-reperfusion injuries.
authors.affiliation: Department Anesthesiology, Saitama Medical School, Saitama, Japan.
authors.forename: Ikyoko
authors.initials: I
authors.lastname: Kuroki
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Isatoshi
authors.initials: I
authors.lastname: Mizukami
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Isao
authors.initials: I
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000503:physiopathology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase