[Establishment of detection method for HCMVpp65 of the blood donors and its application in blood bank].
To establish method suitable to assay HCMVpp65 of the blood donors in blood bank and to supply safe blood to the patients, the immunocytochemical techniques were used, (6 - 8) x 10(6)/ml cells were counted, 50 x 10(3) cells were detected by light microscope, The results showed that 10 positive samples in 103 samples were found, positive rate was 9.71%, among 10 positive samples, 2 samples were still positive in the second detecting. In conclusion, this method is simple, quick and effective, suitable to detect HCMVpp65 of the blood donors in the blood bank.
Liu
Yan-Chun
YC
Jiangsu Province Blood Center, Nanjing 210042, China.
Liu
Hon-Li
HL
Liu
Yi
Y
Tang
Rong-Cai
RC
Sheng
Da-Xiang
DX
D001771:Blood Banks; D001782:Blood Donors; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010750:Phosphoproteins / Q000097:blood*; D014763:Viral Matrix Proteins / Q000097:blood*
D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C464963:pp67 protein, human cytomegalovirus