Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT.
Matsumura
Hiroyuki
H
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Hasuwa
Hidetoshi
H
Inoue
Naokazu
N
Ikawa
Masahito
M
Okabe
Masaru
M
D000818:Animals; D019070:Cell Lineage; D004167:Diphtheria Toxin / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009929:Organ Size; D009928:Organ Specificity; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D004167:Diphtheria Toxin; D010446:Peptide Fragments; D014764:Viral Proteins; C029795:diphtheria toxin fragment A; C045073:Cre recombinase; D019426:Integrases