Synthesis of DNA using a new two-step cycle.
For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5'-carbonate protected 2'-deoxynucleoside-3'-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.
Dellinger
Douglas J
DJ
Agilent Laboratories, Fort Collins, CO, USA.
Betley
Jason R
JR
Wyrzykiewicz
Tadeusz K
TK
Caruthers
Marvin H
MH
D000577:Amides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000577:Amides; D007202:Indicators and Reagents; D010756:Phosphoric Acids; D004247:DNA; C011067:phosphoramidic acid