Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus induces Hz-AM1 cell cycle arrest at the G2 phase with accumulation of cyclin B1.
The cell cycle phase distributions of Hz-AM1 cells grown in monolayer culture were G1 = 49.7 +/- 3.3%, S = 22.7 +/- 3.8% and G2/M = 27.8 +/- 4.2% without >4N DNA content. The culture doubling time was about 40 h and the duration of the G1, S and G2/M phases was estimated to be 10, 14 and 16 h, respectively. HaSNPV infection of Hz-AM1 cells resulted in both unsynchronized and synchronized G1 phase arrest at G2/M phase. HaSNPV infection also resulted in the appearance of more than 4N DNA content, which accumulated to the highest levels 72 h post-infection. We also found that the expression level of cyclin B1 increased significantly after 16 h post-infection, while cyclin A did not show any change. This observation supports the Hz-AM1-infected arrest at the G2/M phage. Cytoplasm location of cyclin B1 indicated that the Hz-AM1 cell cycle arrest was at the G2 phase.
Zhou
Rui
R
Institution of Entomology, Central China Normal University, Wuhan 430070, PR China.
Yu
Ze-Hua
ZH
Li
Xiao-qing
XQ
Jia
Fang
F
Wu
Jian-hong
JH
Chen
Xinwen
X
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D019925:Cyclin A / Q000378:metabolism; D019926:Cyclin B / Q000378:metabolism*; D056744:Cyclin B1; D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D016195:G2 Phase; D009036:Moths / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D013997:Time Factors
D019925:Cyclin A; D019926:Cyclin B; D056744:Cyclin B1; D004279:DNA, Viral