[Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus].
BACKGROUND
To study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).


METHODS
Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.


RESULTS
The inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies.


CONCLUSIONS
The ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.
Yan
Qing-guo
QG
Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Huang
Gao-sheng
GS
Guo
Ying
Y
Wang
Zhe
Z
Feng
Ji-liang
JL
Yang
Guo-rong
GR
D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000737:chemistry* / Q000276:immunology / Q000821:virology; D042282:Microdissection; D012469:Salivary Glands / Q000737:chemistry / Q000276:immunology / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral