Analysis of fluorescence excitation-emission matrix of multicomponent drugs. A case study with human placental extract used as wound healer.
The possibility of finger printing of multi component drugs, where a number of components are fluorescent, has been explored after generation of excitation-emission matrix (EEM) contour plots. With nicotinamide adenine dinucleotide, reduced form (NADH) and N-acetyl tryptophanamide (NATA) serving as reference compounds, the contour widths were found to be proportional to fluorophore concentration. EEM generated from different batches of processed human placental extract, used as wound healer, in presence and absence of extraneous fluorescence probes like 1-anilino 8-naphthalenesulphonic acid (1-ANS) or dansyl chloride were found to be highly consistent. Variation of contour widths of these EEM after excitation at 280 or 340 nm were essentially zero subject to the limit of detection of variation (n = 5). This high degree of consistency between batches has been supported from analysis with such independent techniques like UV-absorption spectra, Fourier Transformed IR spectra, TLC and reverse phase HPLC. The advantages of EEM analysis have been discussed.
Datta
Piyali
P
Department of Drug Design, Development and Molecular Modeling, Indian Institute of Chemical Biology, 4 Raja S.C. Mallick Road, Jadavpur, Calcutta 700032, India. p_datt@rediffmail.com
Bhattacharyya
Debasish
D
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes; D006801:Humans; D010924:Placental Extracts / Q000032:analysis* / Q000627:therapeutic use; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing / Q000187:drug effects*
D005456:Fluorescent Dyes; D010924:Placental Extracts