title: Phosphoproteomic analysis of the developing mouse brain.
abstract: Proper development of the mammalian brain requires the precise integration of numerous temporally and spatially regulated stimuli. Many of these signals transduce their cues via the reversible phosphorylation of downstream effector molecules. Neuronal stimuli acting in concert have the potential of generating enormous arrays of regulatory phosphoproteins. Toward the global profiling of phosphoproteins in the developing brain, we report here the use of a mass spectrometry-based methodology permitting the first proteomic-scale phosphorylation site analysis of primary animal tissue, identifying over 500 protein phosphorylation sites in the developing mouse brain.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Ballif
authors.forename: Judit
authors.initials: J
authors.lastname: Villén
authors.forename: Sean A
authors.initials: SA
authors.lastname: Beausoleil
authors.forename: Daniel
authors.initials: D
authors.lastname: Schwartz
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011247:Pregnancy; D020543:Proteome; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D020543:Proteome