Oxidation of tyrosine diketopiperazine to DOPA diketopiperazine with tyrosine hydroxylase.
The diketopiperazine of DOPA was synthesized in high yield from the diketopiperazine of tyrosine using PC12 cell lysate, which expresses high levels of tyrosine hydroxylase. This represents the first use of this enzyme to prepare DOPA-containing peptides.
Saleh
Maysoon B
MB
Department of Chemistry and Biochemistry, Center of Excellence in Biomedical and Marine Biotechnology, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431, USA.
Kerr
Russell G
RG
D000818:Animals; D004295:Dihydroxyphenylalanine / Q000096:biosynthesis*; D054659:Diketopiperazines; D015394:Molecular Structure; D010084:Oxidation-Reduction; D016716:PC12 Cells; D010455:Peptides / Q000138:chemical synthesis; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D054659:Diketopiperazines; D010455:Peptides; D010879:Piperazines; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D014446:Tyrosine 3-Monooxygenase