title: Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout.
abstract: The sequences and gene organisation of two LEAP-2 molecules (LEAP-2A and LEAP-2B) from rainbow trout, Oncorhynchus mykiss are presented. Both genes consist of a 3 exon/2 intron structure, with exon sizes comparable to known mammalian genes. LEAP-2A notably differs from LEAP-2B in having larger introns and a larger 3' UTR. The predicted proteins contain a signal peptide and prodomain, followed by a mature peptide of 41 aa containing four conserved cysteines. The RXXR cleavage site to release the mature peptide was also conserved. Both genes were found to be constitutively expressed in the liver, with expression in the intestine, and to a lesser extent the skin, evident after bacterial challenge.
authors.affiliation: Department of Zoology, Scottish Fish Immunology Research Centre, University of Aberdeen, Zoology Building, Tillydrone Avenue, Aberdeen AB24 2TZ, UK.
authors.forename: Yong-An
authors.initials: YA
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.forename: Chin-I
authors.initials: CI
authors.lastname: Chang
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Secombes
mesh_terms: D000333:Aeromonas / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D064451:Hepcidins; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000235:genetics* / Q000276:immunology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; D011506:Proteins; D012313:RNA