[Isolation of ABA-regulated genes in Oryza sativa through fluorescent differential display PCR (FDD-PCR)].
Abscisic acid (ABA) was critical in plant seed development and response to environmental factors such as stress situations. To study the possible ABA related signaling transduction pathways, we tried to isolate the ABA-regulated genes through fluorescent differential display PCR (FDD-PCR) technology using rice seedling as materials (treated with ABA for 2, 4, 8 and 12h). In the 17 fragments isolated, 14 and 3 clones were up-and down-regulated respectively. Sequence analyses revealed that the encoded proteins were involved in photosynthesis (7 fragments), signal transduction (1 fragments), transcription (2 fragments), metabolism and resistance (6 fragments), and unknown protein (1 fragments). 3 clones, encoding putative alpha/beta hydrolase fold, putative vacuolar H+ -ATPase B subunit, putative tyrosine phosphatase, were confirmed to be regulated under ABA treatment by RT-PCR and northern blot analysis. FDD-PCR and possible functional mechanisms of ABA were discussed.
Xu
Shou Ling
SL
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, The Chinese Academy of Sciences, Shanghai 200032.
Shen
Si Shi
SS
Xu
Zhi Hong
ZH
Xue
Hong Wei
HW
D000040:Abscisic Acid / Q000494:pharmacology*; D015152:Blotting, Northern; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D010937:Plant Growth Regulators; D000040:Abscisic Acid