title: A validated chiral HPLC method for the enantiomeric separation of tolterodine tartarate.
abstract: An isocratic chiral HPLC method was developed for the separation of tolterodine tartarate enantiomers. The mobile phase consists of n-hexane and isopropyl alcohol in the ratio of 980:20 (v/v) with 1 ml diethylamine and 0.6 ml trifluoroacetic acid. Chiralcel OD-H (250 mm x 4.6mm) column was used at constant room temperature. Flow rate was kept at 0.5 ml/min. This method is capable of detecting the S-isomer up to 0.1 microg/ml. The method was validated in terms of linearity, precision, limit of detection (LOD) and limit of quantification (LOQ).
authors.affiliation: Dr. Reddy's Laboratories Ltd., Bulk Actives Unit-III, IDA-Bollaram, Hyderabad 502325, AP, India.
authors.forename: Y Ravindra
authors.initials: YR
authors.lastname: Kumar
authors.forename: G
authors.initials: G
authors.lastname: Ramulu
authors.forename: V V
authors.initials: VV
authors.lastname: Vevakanand
authors.forename: Gopal
authors.initials: G
authors.lastname: Vaidyanathan
authors.forename: Keesari
authors.initials: K
authors.lastname: Srinivas
authors.forename: M Kishore
authors.initials: MK
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Mukkanti
authors.forename: M Satyanarayana
authors.initials: MS
authors.lastname: Reddy
authors.forename: S
authors.initials: S
authors.lastname: Venkatraman
authors.forename: M V
authors.initials: MV
authors.lastname: Suryanarayana
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000737:chemistry*; D015394:Molecular Structure; D010665:Phenylpropanolamine / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D000068737:Tolterodine Tartrate
chemical_list: D001559:Benzhydryl Compounds; D003408:Cresols; D010665:Phenylpropanolamine; D000068737:Tolterodine Tartrate