title: Survival of Apligraf in acute human wounds.
abstract: Apligraf consists of bovine collagen dermis seeded with allogeneic male fibroblasts and keratinocytes. It is been shown to promote healing, but the length of persistence and pathological features have not been characterized previously in acute wounds. Forty-eight deep dermal wounds were created and Apligraf, a split-skin graft (SSG), or a dressing was applied. Biopsies of wounds were taken for immunohistochemical analysis and polymerase chain reaction was performed to detect the Y chromosome from Apligraf cells in 14 female wounds. Male allogeneic DNA was detected in wounds for the first 4 weeks. All subsequent time points were negative apart from one biopsy at 6 weeks. The wounds took 4-9 weeks to heal, with the Apligraf exhibiting no features of engraftment. This was in contrast to the rapid healing seen in the SSG control group. Histology revealed a more intense cellular infiltrate, but less vascularization below Apligraf compared with controls. Evidence of an epidermal-mesenchymal interaction was observed. This is the first article to elucidate the survival of Apligraf allogeneic cells in acute wounds in immunocompetent human subjects for up to 6 weeks and demonstrates that in the management of acute surgical wounds, Apligraf has a role only as a temporary biological dressing.
authors.affiliation: Centre for Cutaneous Research, Barts and The London, Queen Mary's School of Medicine and Dentistry, London E1 2AT, UK.
authors.forename: M
authors.initials: M
authors.lastname: Griffiths
authors.forename: N
authors.initials: N
authors.lastname: Ojeh
authors.forename: R
authors.initials: R
authors.lastname: Livingstone
authors.forename: R
authors.initials: R
authors.lastname: Price
authors.forename: H
authors.initials: H
authors.lastname: Navsaria
mesh_terms: D021522:Bioartificial Organs; D001684:Biological Dressings; D002478:Cells, Cultured; D003094:Collagen / Q000627:therapeutic use*; D005260:Female; D005347:Fibroblasts / Q000473:pathology* / Q000637:transplantation; D006085:Graft Survival / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000473:pathology* / Q000637:transplantation; D008297:Male; D017768:Skin, Artificial; D016896:Treatment Outcome; D014950:Wounds, Penetrating / Q000473:pathology* / Q000628:therapy*
chemical_list: C117898:Apligraf; D003094:Collagen