Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.
Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. The enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and the immobilized toxins were probed with monoclonal antibodies. The antibodies 2A3 and 1A8 allowed the specific detection of the B component of HBL and the nheA component of NHE. The assay enabled the reliable identification of HBL expressing colonies and differentiation from NHE producing but HBL negative colonies.
Moravek
Maximilian
M
Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, 85764 Oberschleissheim, Munich, Germany. m.moravek@mh.vetmed.uni-muenchen.de
Wegscheider
Monika
M
Schulz
Anja
A
Dietrich
Richard
R
Bürk
Christine
C
Märtlbauer
Erwin
E
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001409:Bacillus cereus / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D006460:Hemolysin Proteins; D006461:Hemolysis; D015151:Immunoblotting / Q000379:methods*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; C083961:hemolysin BL protein, Bacillus