A non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean.
A non-specific lipid transfer peptide (nsLTP) with antimicrobial activity was isolated from the mung bean (Phaseolus mungo) seeds. The procedure entailed aqueous extraction, ion exchange chromatography on CM-Sephadex and high performance liquid chromatography (HPLC) on POROS-HS-20. The peptide exhibited a molecular mass of 9.03 kDa in mass spectrometry. It exerted antifungal action toward Fusarium solani, Fusarium oxysporum, Pythium aphanidermatum and Sclerotium rolfsii, and antibacterial action against Staphylococcus aureus but not against Salmonella typhimurium. The lipid binding of this peptide was very similar to that of a previously described lipid transfer protein extracted from wheat seeds and maize seeds, indicating that it possessed lipid transfer activity. The present findings add to the scarcity of the literature on leguminous nsLTPs.
Wang
Shao Yun
SY
Insititute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
Wu
Jin Hong
JH
Ng
T B
TB
Ye
Xiu Yun
XY
Rao
Ping Fan
PF
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007887:Fabaceae / Q000201:enzymology*; D005658:Fungi / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002352:Carrier Proteins; C072087:sterol carrier proteins