title: A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans.
abstract: Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5' ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchus -specific genes were identified, and their implications for nematode control strategies are discussed.
authors.affiliation: Genome Sequencing Center, Department of Genetics, Washington University School of Medicine, MO 63108, St. Louis, USA, mmitreva@watson.wustl.edu
authors.forename: M
authors.initials: M
authors.lastname: Mitreva
authors.forename: A A
authors.initials: AA
authors.lastname: Elling
authors.forename: M
authors.initials: M
authors.lastname: Dante
authors.forename: A P
authors.initials: AP
authors.lastname: Kloek
authors.forename: A
authors.initials: A
authors.lastname: Kalyanaraman
authors.forename: S
authors.initials: S
authors.lastname: Aluru
authors.forename: S W
authors.initials: SW
authors.lastname: Clifton
authors.forename: D McK
authors.initials: DM
authors.lastname: Bird
authors.forename: T J
authors.initials: TJ
authors.lastname: Baum
authors.forename: J P
authors.initials: JP
authors.lastname: McCarter
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D022761:Gene Transfer, Horizontal; D017238:Genes, Helminth; D005810:Multigene Family; D010641:Phenotype; D010935:Plant Diseases / Q000469:parasitology; D010944:Plants / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D018510:RNA, Helminth / Q000235:genetics*; D020038:RNA, Spliced Leader / Q000235:genetics*; D013045:Species Specificity; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity
chemical_list: D018509:DNA, Helminth; D018510:RNA, Helminth; D020038:RNA, Spliced Leader