title: Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8.
abstract: Ribosomal protein L27 is located near the peptidyltransferase center at the interface of ribosomal subunits, and is important for ribosomal assembly and function. We report the crystal structure of ribosomal protein L27 from Thermus thermophilus HB8, which was determined by the multiwavelength anomalous dispersion method and refined to an R-factor of 19.7% (R(free) = 23.6%) at 2.8 A resolution. The overall fold is an all beta-sheet hybrid. It consists of two sets of four-stranded beta-sheets formed around a well-defined hydrophobic core, with a highly positive charge on the protein surface. The structure of ribosomal protein L27 from T. thermophilus HB8 in the RNA-free form is investigated, and its functional roles in the ribosomal subunit are discussed.
authors.affiliation: RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Wang
authors.forename: Chie Hori
authors.initials: CH
authors.lastname: Takemoto
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Murayama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sakai
authors.forename: Ayako
authors.initials: A
authors.lastname: Tatsuguchi
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012269:Ribosomal Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D016963:Thermus thermophilus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012269:Ribosomal Proteins; C034661:ribosomal proteins L27