title: Mitochondrial effects of HIV infection on the peripheral blood mononuclear cells of HIV-infected patients who were never treated with antiretrovirals.
abstract: To investigate the effects of HIV infection on mitochondrial DNA (mtDNA) content and other mitochondrial parameters, we used peripheral blood mononuclear cells (PBMCs) from 25 asymptomatic antiretroviral-naive human immunodeficiency virus (HIV)-infected patients and from 25 healthy control subjects. HIV-infected patients had significant decreases in mtDNA content (decrease, 23%; P<.05) and in the activities of mitochondrial respiratory chain (MRC) complex II (decrease, 41%; P<.001), MRC complex III (decrease, 38%; P<.001), MRC complex IV (decrease, 19%; P=.001), and glycerol-3-phosphate dehydrogenase (decrease, 22%; P<.001), along with increased lipid peroxidation of PBMC membranes (P=.007). Therefore, HIV infection is associated not only with mtDNA depletion, but also with extensive MRC disturbances and increased oxidative damage.
authors.affiliation: Mitochondrial Research Laboratory, Muscle Research Unit, Department of Internal Medicine, Hospital Clínic, Villarroel 170, 08036 Barcelona, Catalonia, Spain. omiro@clinic.ub.es
authors.forename: Oscar
authors.initials: O
authors.lastname: Miró
authors.forename: Sònia
authors.initials: S
authors.lastname: López
authors.forename: Esteban
authors.initials: E
authors.lastname: Martínez
authors.forename: Enric
authors.initials: E
authors.lastname: Pedrol
authors.forename: Ana
authors.initials: A
authors.lastname: Milinkovic
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Deig
authors.forename: Glòria
authors.initials: G
authors.lastname: Garrabou
authors.forename: Jordi
authors.initials: J
authors.lastname: Casademont
authors.forename: Josep M
authors.initials: JM
authors.lastname: Gatell
authors.forename: Francesc
authors.initials: F
authors.lastname: Cardellach
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004272:DNA, Mitochondrial / Q000378:metabolism; D005260:Female; D015658:HIV Infections / Q000473:pathology*; D006679:HIV Seropositivity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000821:virology*; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000821:virology*; D019562:Viral Load
chemical_list: D004272:DNA, Mitochondrial