Preconcentration technique for nonylphenol using cellulose cotton with homogenous liquid-liquid extraction for liquid chromatographic analysis.
A powerful preconcentration method for nonylphenol (NP) has been developed for liquid-chromatography by combining the use of cellulose cotton (solid-phase extraction) with homogeneous liquid-liquid extraction. A 100 ml of sample solution was preconcentrated using cotton, and the eluate obtained (acetonitrile; 5 ml) was further preconcentrated to 50 microl within 10 min using a homogeneous liquid-liquid extraction method (volume ratio, 2,000-fold; 100 ml--> 50 microl). The sample concentration increases from preconcentration was 1,599-fold, and NP was extracted into the sedimented phase at 80%. The proposed method was applied to high performance liquid chromatography with fluorescence detection (FL/HPLC); the lowest determination limit obtained was 1.0 x 10(-9) mol l(-1).
Takagai
Yoshitaka
Y
Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa 4-12-1, Hitachi, Ibaraki, 316-8511, Japan.
Kubota
Toshio
T
Akiyama
Ryoutaro
R
Aoyama
Eiji
E
Igarashi
Shukuro
S
D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D010636:Phenols; C025256:nonylphenol; D002482:Cellulose