The secretion signal of YopN, a regulatory protein of the Yersinia enterocolitica type III secretion pathway.
The type III secretion signal of Yersinia enterocolitica YopN was mapped using a gene fusion approach. yopN codons 1 to 12 were identified as critical for signal function. Several synonymous mutations that abolish secretion of hybrid proteins without altering the codon specificity of yopN mRNA were identified.
Goss
John W
JW
Committee on Microbiology, The University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA.
Sorg
Joseph A
JA
Ramamurthi
Kumaran S
KS
Ton-That
Hung
H
Schneewind
Olaf
O
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics*; D001692:Biological Transport; D004252:DNA Mutational Analysis; D005798:Genes, Bacterial; D017930:Genes, Reporter; D019868:Kanamycin Kinase / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity
D001426:Bacterial Proteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals; C070015:YopN protein, Yersinia; D019868:Kanamycin Kinase