Promoting the cytocompatibility of polyurethane scaffolds via surface photo-grafting polymerization of acrylamide.
Polyurethane (PU) porous scaffolds were modified by grafting polymerization of acrylamide (AAm) initiated under UV light. A pre-adsorbing-monomer method was used beforehand. FTIR-ATR spectroscopy and X-ray photoelectron spectroscopy (XPS) measurements confirmed the presence of grafted PAAm on PU scaffolds. The measurement of water adsorption demonstrated the improvement of hydrophilicity after PU scaffolds were grafted with polyacrylamide (PAAm). The PAAm grafting degree related to the amount of AAm adsorbed, average pore diameter, and the degree of porosity. This study shows that higher degree of porosity and bigger porous areas yielded larger amounts of absorbed AAm and higher grafting degrees. In vitro human endothelial cell cultures of PU scaffolds modified with hydrophilic PAAm showed better cytocompatibility than the control matrix.
Zhu
Yabin
Y
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Gao
Changyou
C
Guan
Jianjun
J
Shen
Jiacong
J
D000180:Acrylic Resins; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010777:Photochemistry; D011140:Polyurethanes; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014466:Ultraviolet Rays; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011140:Polyurethanes; D000099166:Polyacrylamides