title: Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows.
abstract: OBJECTIVE
  To evaluate L-selectin (CD62L) and Mac-1 (CD11b) expression at the surface of blood and milk neutrophils during the early inflammatory response to Escherichia coli-induced mastitis in cows.
  
  
  METHODS
  6 healthy Holstein heifers in early lactation.
  
  
  METHODS
  Blood and milk samples were collected before and after intramammary administration of 10(4) CFU's of E coli in the left mammary gland quarters. Bacterial counts and electrolyte concentrations in milk, rectal temperature, differential blood leukocyte counts, milk somatic cell counts, neutrophil viability, and the expression of CD62L and CD11b on blood and milk neutrophils were determined longitudinally.
  
  
  RESULTS
  Bacteria grew during the first 6 hours after inoculation with a pronounced leukocytic influx. Coincident with neutrophil influx was an increase in CD62L+ and CD11b+ milk neutrophils, as well as an improved viability of milk neutrophils. The peak of the inflammatory reaction was reached approximately 12 hours after E coli inoculation. From that time forward, changes in CD62L and CD11b expression were opposed to each other, with a decrease in CD62L expression and an increase in CD11b expression on blood and milk neutrophils; the magnitude of the differences in CD62L and CD11b expression between blood and milk neutrophils decreased. Percentages of CD62L+ and CD11b+ milk neutrophils increased to percentages that were similar to blood neutrophils (ie, approx 92%).
  
  
  CONCLUSIONS
  The presence of adhesion molecules on a large percentage of milk neutrophils during the acute inflammatory response, together with the changes in receptor density, suggest a major role for CD62L and CD11b in neutrophil function during coliform mastitis.
authors.affiliation: Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Araceli
authors.initials: A
authors.lastname: Diez-Fraille
authors.forename: Jalil
authors.initials: J
authors.lastname: Mehrzad
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
authors.forename: Luc
authors.initials: L
authors.lastname: Duchateau
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
mesh_terms: D000818:Animals; D001769:Blood / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D019041:L-Selectin / Q000276:immunology*; D007958:Leukocyte Count; D016177:Macrophage-1 Antigen / Q000276:immunology*; D008414:Mastitis, Bovine / Q000276:immunology* / Q000382:microbiology; D008892:Milk / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen; D019041:L-Selectin