title: A RecA filament capping mechanism for RecX protein.
abstract: The RecX protein is a potent inhibitor of RecA protein activities. RecX functions by specifically blocking the extension of RecA filaments. In vitro, this leads to a net disassembly of RecA protein from circular single-stranded DNA. Based on multiple observations, we propose that RecX has a RecA filament capping activity. This activity has predictable effects on the formation and disassembly of RecA filaments. In vivo, the RecX protein may limit the length of RecA filaments formed during recombinational DNA repair and other activities. RecX protein interacts directly with RecA protein, but appears to interact in a functionally significant manner only with RecA filaments bound to DNA.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Julia C
authors.initials: JC
authors.lastname: Drees
authors.forename: Shelley L
authors.initials: SL
authors.lastname: Lusetti
authors.forename: Sindhu
authors.initials: S
authors.lastname: Chitteni-Pattu
authors.forename: Ross B
authors.initials: RB
authors.lastname: Inman
authors.forename: Michael M
authors.initials: MM
authors.lastname: Cox
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001665:Binding Sites / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D011485:Protein Binding / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C430523:RecX protein, Xanthomonas campestris; D000251:Adenosine Triphosphatases; D004265:DNA Helicases