Phylogenetic relationship of porcine endogenous retrovirus (PERV) in Chinese pigs with some type C retroviruses.
PCR amplification of proviral DNA extracted from peripheral blood lymphocytes of three Chinese pigs (Banna minipig inbreed (BMI), Wu-Zhi-Shan pig (WZSP) and Neijiang pig (NJP)), using primers corresponding to highly conserved regions of reverse transcriptase (RT) of pol gene and nucleocapsid sequence of gag gene. PCR products were then extracted and cloned into pGEM-T vector. Phylogenetic analysis of the nucleotide sequences of PERV-BMI, PERV-WZSP and PERV-WZSP revealed that they were of retroviral origin. Phylogenetic trees were constructed from the translated amino acids of PERVs and other type C retrovirus, as well as lentivirus of GenBank. The research demonstrated that PERVs of Chinese pigs and other PERVs were closely related to other pathogenic type C retroviruses. From the gag analysis, a novel subgroup of PERV was identified and this novel sequence described in this report would allow such investigation to be actively pursued.
Li
Zhang
Z
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, PR China.
Ping
Yu
Y
Shengfu
Li
L
Hong
Bu
B
Youping
Li
L
Yangzhi
Zeng
Z
Jingqiu
Cheng
C
D000818:Animals; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D020077:Endogenous Retroviruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017988:Gammaretrovirus / Q000145:classification / Q000235:genetics*; D015750:Genes, gag; D015751:Genes, pol; D016086:Lentivirus / Q000235:genetics; D008214:Lymphocytes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000821:virology*
D004279:DNA, Viral