title: Divergent effects of caloric restriction on gene expression in normal and long-lived mice.
abstract: Long-lived Ames dwarf mice share many phenotypic characteristics with animals subjected to caloric restriction (CR) but they are not CR mimetics. CR prolongs longevity in both normal and Ames dwarf mice. Using real-time polymerase chain reaction and western blot, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice subjected to 30% CR. The results revealed divergent responses of dwarf and normal animals to CR raising an interesting possibility that CR may affect longevity of normal and dwarf mice by different mechanisms. Moreover, effects of dwarfism on the expression of the examined genes differed from the effects of CR, thus adding to the evidence that these long-lived mutants are not CR mimetics.
authors.affiliation: Department of Internal Medicine, Southern Illinois University, School of Medicine, Springfield 62794-9628, USA. mmasternak@siumed.edu
authors.forename: Michal M
authors.initials: MM
authors.lastname: Masternak
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Regaiey
authors.forename: Michael S
authors.initials: MS
authors.lastname: Bonkowski
authors.forename: Jacob
authors.initials: J
authors.lastname: Panici
authors.forename: Liou
authors.initials: L
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Grzegorz K
authors.initials: GK
authors.lastname: Przybylski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bartke
mesh_terms: D000818:Animals; D015153:Blotting, Western; D031204:Caloric Restriction; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D007328:Insulin / Q000502:physiology; D008136:Longevity / Q000502:physiology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D016133:Polymerase Chain Reaction
chemical_list: D007328:Insulin