Solid-phase synthesis of circular oligonucleotides.
A protocol for the straightforward preparation of small circular oligodeoxyribonucleotides (2-28 nt) is reported. The assembly of the oligonucleotide chain (standard phosphoramidite chemistry) and cyclization by the phosphotriester method take place on a tailor-made nucleotide-derivatized solid support. Although cyclization yields are moderate, the procedure exploits a synthesis design that allows selective cleavage of the circular oligonucleotide from the support, which facilitates isolation of the target molecule by simple filtration.
Pedroso
Enrique
E
Department de Química Orgánica, Universitat de Barcelona, Spain.
Escaja
Nuria
N
Frieden
Miriam
M
Grandas
Anna
A
D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000138:chemical synthesis*
D009841:Oligonucleotides