Enzyme assays for high-throughput screening.
Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic activity is detected using labelled substrates or indirect sensor systems that produce a detectable spectroscopic signal upon reaction. Recent advances in the development of high-throughput enzyme assays have identified new labels and chromophores to detect a wide range of enzymes activities. Enzyme activity profiling and fingerprinting have also been used as tools for identification and classification, while microarray formats have been devised to increase throughput.
Goddard
Jean-Philippe
JP
Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
Reymond
Jean-Louis
JL
D002384:Catalysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004798:Enzymes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004798:Enzymes