title: Biocompatibility of two commercial forms of mineral trioxide aggregate.
abstract: OBJECTIVE
  To examine the biocompatibility of two commercial forms of mineral trioxide aggregate (MTA), by evaluating the morphology of an established cell line.
  
  
  METHODS
  The two cements were cast on glass cover slips and cured for 1 or 28 days. Saos-2 osteosarcoma cells were trypsinized and seeded at a density of 1 x 10(5) cells and were then placed in medium over the material-coated coverslips for 1, 5 and 7 days. After these time intervals the media were discarded and the cells fixed. Cell morphological investigation was performed by scanning electron microscopy at various magnifications ranging from x 250 to x 500. The biocompatibility of cement constituents, alusilicate flux and bismuth oxide was also investigated.
  
  
  RESULTS
  All cement samples cured for 1 day showed a confluent cell monolayer after 5 and 7 days. The response to both materials was similar. Materials cured for 28 days showed incomplete cell confluence after 1 and 5 days. Alusilicate flux and bismuth oxide did not demonstrate biocompatibility.
  
  
  CONCLUSIONS
  The 1-day cured samples of two commercial forms of MTA showed good biocompatibility. However, the 28-day cured samples were less biocompatible after 1 and 5 days.
authors.affiliation: Department of Conservative Dentistry, Guy's, King's and St Thomas' Dental Institute, King's College London, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Camilleri
authors.forename: F E
authors.initials: FE
authors.lastname: Montesin
authors.forename: S
authors.initials: S
authors.lastname: Papaioannou
authors.forename: F
authors.initials: F
authors.lastname: McDonald
authors.forename: T R
authors.initials: TR
authors.lastname: Pitt Ford
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000538:Aluminum Silicates / Q000633:toxicity; D001729:Bismuth / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity*; D045744:Cell Line, Tumor; D048429:Cell Size / Q000187:drug effects; D004338:Drug Combinations; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010087:Oxides / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D000538:Aluminum Silicates; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C033301:bismuth oxide; D001729:Bismuth