title: Removal of carbonaceous and nitrogenous pollutants from a synthetic wastewater using a membrane-coupled bioreactor.
abstract: Two modified Ludzack-Ettinger (MLE)-type membrane-coupled bioreactors (MBRs) were investigated in this study for the purpose of removing both nitrogenous and carbonaceous pollutants from a synthetic wastewater. During the first MBR experiment, removal efficiencies were high (>90%) for chemical oxygen demand (COD) and ammonia, but total nitrogenous pollutant removal efficiency was poor (approximately 25%). Bacterial community analysis of ammonia oxidizing bacteria (AOB) by a nested PCR-DGGE approach detected two Nitrosomonas-like populations and one Nitrosospira-like population. During the initial portion of the second MBR experiment, COD and ammonia removal efficiencies were similar to the first MBR experiment until the COD of the influent wastewater was increased to provide additional electron donors to support denitrification. Total nitrogen removal efficiencies eventually exceeded 90%, with a hydraulic residence time (HRT) of 24 h and a recirculation ratio of 8. When the HRT of the MBR experiment was decreased to 12 h, however, ammonia removal efficiency was adversely affected. A subsequent increase in the HRT to 18 h helped improve removal efficiencies for both ammonia (>85%) and total nitrogenous compounds (approximately 70%). Our research demonstrates that MBRs can be effectively designed to remove both carbonaceous and nitrogenous pollutants. The ability of the microbial community to switch between anoxic (denitrifying) and oxic (nitrifying) conditions, however, represents a critical process constraint for the application of MLE-type MBR systems, such that little benefit is gained compared to conventional designs.
authors.affiliation: Department of Civil Engineering, University of Minnesota, 500 Pillsbury Drive SE, 122 CivE, Minneapolis, MN 55455, USA.
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Ghosh
authors.forename: Timothy M
authors.initials: TM
authors.lastname: LaPara
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D017672:Nitrogen Compounds / Q000378:metabolism*; D043369:Nitrosomonadaceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009606:Nitrosomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D009930:Organic Chemicals / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D009566:Nitrates; D009573:Nitrites; D017672:Nitrogen Compounds; D009930:Organic Chemicals; D011506:Proteins; D014874:Water Pollutants, Chemical; D000641:Ammonia