Simple and sensitive fluorimetric liquid chromatography method for the determination of valproic acid in plasma.
A simple and sensitive liquid chromatographic method is described for the analysis of valproic acid in human plasma. The method is based on the derivatization of valproic acid extracted from acidified plasma with 2-(2-naphthoxy)ethyl 2-(piperidino)ethanesulfonate. The resulting derivative is highly responsive to a fluorimetric detector (excitation at 230 nm and emission at 350 nm), giving a low detection limit of 0.6 microM (S/N = 3, 10 microl injected). The relative standard deviations of the method for intra- and inter-day analyses (n = 5) are below 3.3 and 4.1%, respectively. Toluene was used for the extraction of valproic acid from plasma and the toluene extract obtained was subjected to subsequent derivatization without solvent replacement. The simple method was applied to the analysis of valproic acid in plasma of dosed patients using only small amount of sample (10-50 microl plasma).
Lin
Ming-Chun
MC
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Kou
Hwang-Shang
HS
Chen
Cheng-Chung
CC
Wu
Shou-Mei
SM
Wu
Hsin-Lung
HL
D000328:Adult; D000927:Anticonvulsants / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014635:Valproic Acid / Q000097:blood*
D000927:Anticonvulsants; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012996:Solutions; D014635:Valproic Acid