Hematopoietic development of human embryonic stem cells in culture.
The isolation of embryonic stem (ES) cells from human preimplantation blastocysts creates an exciting new starting point to analyze the earliest stages of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.
Tian
Xinghui
X
Stem Cell Institute and Department of Medicine, University of Minnesota, Minneapolis, USA.
Kaufman
Dan S
DS
D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D013234:Stem Cells / Q000166:cytology*