[Prolactin-binding activity of bovine granulosa cells on luteinization and exposure to somatotropin in vitro].
The influence of luteinization and bovine somatotropin (ST, 5-50 ng/ml) during cultivation of bovine granulosa cells on their ability to bind [125I]-labeled bovine prolactin (PRL) was studied. On the second day of cultivation in serumfree medium, granulosa cells from immature antral follicles underwent spontaneous luteinization, in both the absence and presence of ST. The level of [125I]-PRL specific binding to cells increased after two days of cultivation, with a negative correlation being revealed between estradiol production by the cells and their PRL-binding activity. At the same time, the addition of ST to the culture medium had no effect on the level of [125I]-PRL specific binding to native and luteinizing granulosa cells. The findings suggest a stimulatory influence of the luteal differentiation process on the PRL-binding activity of bovine granulosa cells, this influence is independent of the action of ST.
Lebedeva
I Iu
IIu
Kuz'mina
T I
TI
Lebedev
V A
VA
Schneider
F
F
Alm
H
H
Torner
H
H
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology; D007457:Iodine Radioisotopes; D034461:Luteinization / Q000502:physiology; D011388:Prolactin / Q000378:metabolism*; D011485:Protein Binding
D007457:Iodine Radioisotopes; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone