[The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes].
OBJECTIVE
To observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level.


METHODS
In isolated ventricular myocytes of guinea pig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ik1 were observed using the whole cell patch clamp technique.


RESULTS
Ouabain (5 micromol x L(-1)) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ik1 in guinea pig ventricular myocytes. Aconitine (1 micromol x L(-1)) lengthened the APD90, increased ICa-L, decreased Ito and increased Ik1 in rat ventricular myocytes.


CONCLUSIONS
The targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito, and Ik1. APD, ICaL, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine- induced arrhythmic models on cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These two new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.
Gong
Dong-mei
DM
Department of Pharmacology, Harbin Medical University, Harbin 150086, China. gdmxxl@ems.hrbmu.edu.cn
Shan
Hong-li
HL
Zhou
Yu-hong
YH
Dong
De-li
DL
Yang
Bao-feng
BF
D000157:Aconitine / Q000494:pharmacology*; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000473:pathology / Q000503:physiopathology*; D020746:Calcium Channels, L-Type / Q000187:drug effects*; D002469:Cell Separation; D005260:Female; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000473:pathology; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology*; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D020746:Calcium Channels, L-Type; D024661:Potassium Channels, Inwardly Rectifying; D010042:Ouabain; D000157:Aconitine