Tumor neoangiogenesis by CD31 and CD105 expression evaluation in breast carcinoma tissue microarrays.
OBJECTIVE
The aim of the study was to evaluate CD31 and CD105 immunohistochemical expressions in tissue microarrays from 360 breast carcinomas.


METHODS
Computerized (ACIS/Chromavision) assisted image analysis was performed to compare immunoreactions in tissue microarrays with those in current paraffin and frozen sections. We also aimed to determine the CD105 and CD31 prognostic significance and relevance in routine practice by correlating results of immunodetections with patients' (n = 360) outcome (14.3-year follow-up).


RESULTS
The results show (a) that in tissue microarrays, the CD31 and CD105 expression quantified by image analysis device did not correlate with the measurements assessed on routine paraffin sections; (b) that CD105 expression is endowed of a prognostic significance in paraffin sections in terms of overall survival (P < 0.01), whereas in contrast, CD31 on paraffin sections did not correlate with patients overall survival; (c) that semiquantitative analysis of CD105 expression correlated with the image analysis measurements in frozen sections (rho = 0.671, P < 0.01) and paraffin (rho = 0.824, P < 0.01) sections. However, paraffin sections were less immunostained than frozen ones.


CONCLUSIONS
It is concluded (a) that CD105 may be suitable in paraffin sections to evaluated neoangiogenesis; and (b) that tissue microarrays are not suitable substrates for neoangiogenesis evaluation as a prognostic indicator in breast carcinomas, in contrast to current tissue sections.
Charpin
Colette
C
Department of Pathology, Centre Hospitalier, Universitaire Nord, France. colette.charpin@ao-hm.fr
Dales
Jean-Philippe
JP
Garcia
Stéphane
S
Carpentier
Séverine
S
Djemli
Amina
A
Andrac
Lucile
L
Lavaut
Marie-Noëlle
MN
Allasia
Claude
C
Bonnier
Pascal
P
D000230:Adenocarcinoma / Q000098:blood supply* / Q000175:diagnosis; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D015703:Antigens, CD; D001943:Breast Neoplasms / Q000098:blood supply* / Q000175:diagnosis / Q000378:metabolism; D000071063:Endoglin; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016612:Paraffin Embedding; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011379:Prognosis; D011956:Receptors, Cell Surface; D015996:Survival Rate; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1