Heptakis(6-amino-6-deoxy)-beta-cyclodextrin as a chiral selector for the separation of anionic analyte enantiomers by capillary electrophoresis.
A hepta-substituted beta-cyclodextrin bearing seven amino groups, heptakis(6-amino-6-deoxy)-beta-cyclodextrin (per-6-NH2-beta-CD) was successfully used as a chiral selector for the enantioseparation of different anionic analytes. The running buffer pH and chiral selector concentration were the studied parameters crucial in achieving the maximum possible enantioresolution. Enantiomeric separation of a mixture of seven carboxybenzyl-amino acids was achieved in 24 min. Excellent resolution was obtained for carboxybenzyl-tryptophan (Rs = 11.2).
Budanova
Natalia
N
Analytical Chemistry Division, Chemistry Department, Moscow State University, Russia. natasha_budanova@mail.ru
Shapovalova
Elena
E
Lopatin
Sergei
S
Varlamov
Valery
V
Shpigun
Oleg
O
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D000838:Anions; D002021:Buffers; D002240:Carbohydrate Sequence; D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011422:Propionates / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000596:Amino Acids; D000838:Anions; D002021:Buffers; D002264:Carboxylic Acids; D008333:Mandelic Acids; D011422:Propionates; D047392:beta-Cyclodextrins; D014364:Tryptophan; C037938:mandelic acid