An improved method for detection of Leishmania amastigotes by an antibody probe against the small subunit of leishmanial ribonucleotide reductase.
By taking advantage of an antibody raised against the small M2 subunit of ribonucleotide reductase of Leishmania that reacts with the enzyme in the nucleus of the parasite but does not cross-react with the same enzyme of the host macrophage, an improved fluorescence-staining method is developed for enumeration of leishmanial amastigotes inside the macrophage. The method offers an accurate and easy way of counting, compared with Giemsa staining.
Chang
Shu-Ching
SC
Division of infectious diseases, Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan, ROC.
Kuzmenok
Oleg
O
Chiang
Su-Chi
SC
Lee
Sho Tone
ST
D000818:Animals; D000913:Antibodies, Protozoan; D007891:Leishmania / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000469:parasitology; D017737:Macrophages, Peritoneal / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012262:Ribonucleoside Diphosphate Reductase / Q000276:immunology*
D000913:Antibodies, Protozoan; C093713:ribonucleotide reductase M2; D012262:Ribonucleoside Diphosphate Reductase