title: Vaccination of C57/BL6 mice with Dobrava hantavirus nucleocapsid protein in Freund's adjuvant induced partial protection against challenge.
abstract: Dobrava hantavirus (DOBV) causes a severe form of hemorrhagic fever with renal syndrome (HFRS) for which there is no therapy or vaccine available. We compared the immunogenicity and protective efficacy of recombinant DOBV nucleocapsid protein (rDOBV N) given with Alum or Freund's as adjuvant, or PBS, in C57/BL6 mice. All mice given Alum or Freund's seroconverted as did 6/8 mice given rDOBV N with PBS. Reciprocal geometric mean total IgG-titers were 5380, 18,100, and 800, respectively, while the mean IgG1/IgG2a ratios were 17.5, 9.25, and 12, respectively. Furthermore, ELIspot assays showed higher levels of IL-4 producing peripheral blood mononuclear cells (PBMCs) in the group given Alum as compared to the other groups. Interestingly, only mice receiving rDOBV N with Freund's adjuvant were protected from challenge (75% protected), indicating that the strong Th2-type of immune response induced by Alum against rDOBV N did not induce protection in mice.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institutet, S-171 77 Stockholm, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Klingström
authors.forename: Irina
authors.initials: I
authors.lastname: Maljkovic
authors.forename: Bartek
authors.initials: B
authors.lastname: Zuber
authors.forename: Erik
authors.initials: E
authors.lastname: Rollman
authors.forename: Anne
authors.initials: A
authors.lastname: Kjerrström
authors.forename: Ake
authors.initials: A
authors.lastname: Lundkvist
mesh_terms: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000276:immunology / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000097:blood; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D019590:Nucleocapsid Proteins / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007376:Interleukin-2; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D015847:Interleukin-4; C041524:aluminum sulfate; D005620:Freund's Adjuvant