On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays.
Two trials were carried out to compare the use of different enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Sarcoptes scabiei var. suis. In the first trial, we employed four tests and 70 selected sera from a closed pig farm with sarcoptic mange. The sera were taken from the breeding area as well as from the finishing unit and compared with skin scrapings. The SARCOPTES-ELISA 2001 yielded the most positive results (88.58%), followed by the ELISA of the National Veterinary Institute, Uppsala (70%), the Acar-Test P-ELISA (52.86%), skin scrapings (48.57%), and the CHEKIT Sarcoptest (30%). In the second trial, eight litters from infected sows were examined from weeks 1-12 of life using the CHEKIT Sarcoptest and the SARCOPTES-ELISA 2001. The presence of maternal antibodies was highest on day 7 in both ELISAs and could be detected until weeks 5-9 of life. Antibodies increased as a result of an active immune response between days 56 and 63 in the SARCOPTES-ELISA 2001 and between days 70 and 77 of life in the CHEKIT Sarcoptest. Significant differences between the first and second litters were observed.
Löwenstein
M
M
Institute of Parasitology and Zoology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Michael.Loewenstein@vu-wien.ac.at
Kahlbacher
H
H
Peschke
R
R
D000818:Animals; D000906:Antibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D011933:Reagent Kits, Diagnostic; D012520:Sarcoptes scabiei / Q000276:immunology*; D012532:Scabies / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
D000906:Antibodies; D011933:Reagent Kits, Diagnostic