Biodegradation of phenol by acclimatized Pseudomonas putida cells using glucose as an added growth substrate.
Biodegradation of phenol, a pollutant derived from many industrial processes, was achieved through acclimatized Pseudomonas putida cells. The strategy to overcome the inhibitory effect of phenol on microbial growth involved the addition of glucose, a conventional carbon source. A factorial experimental design was employed in order to optimize the initial phenol and glucose concentrations. The optimum conditions found were applied in 2-lt bioreactors. The development of acclimatized cells and the use of glucose as an added growth substrate resulted in a significant phenol degradation rate of 60.7 mg L(-1) h(-1) with a complete removal of 1200 mg L(-1) phenol.
Mamma
Diomi
D
Biotechnology Laboratory, Department of Chemical Engineering, National Technical University of Athens, Athens, Greece.
Kalogeris
Emmanuel
E
Papadopoulos
Nikolaos
N
Hatzinikolaou
Dimitris G
DG
Christrakopoulos
Paul
P
Kekos
Dimitris
D
D001673:Biodegradation, Environmental; D004202:Disinfectants / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007220:Industrial Waste; D019800:Phenol / Q000378:metabolism*; D016958:Pseudomonas putida / Q000254:growth & development / Q000502:physiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
D004202:Disinfectants; D007220:Industrial Waste; D019800:Phenol; D005947:Glucose