title: Evaluation of chiral purity for a substituted imidazole p38 MAP kinase inhibitor and its intermediates using a single chiral capillary electrophoresis method.
abstract: The chiral separation of a substituted imidazole p38 MAP kinase inhibitor and its intermediates was investigated using capillary electrophoresis (CE) with various sulfated cyclodextrins. After initial screens, a single CE chiral method with a randomly sulfated beta-CD was selected for the evaluation of chiral purity for all three compounds. Operational parameters, such as the concentration of the chiral selectors, background electrolyte (or mobile phase) pH, organic modifiers, and temperature were varied in order to achieve an optimized method. The optimal method was validated in terms of linearity, sensitivity, precision, ruggedness, and specificity.
authors.affiliation: Merck Research Laboratories, Merck and Co. Inc., Rahway, NJ 07065, USA. lili_zhou@merck.com
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.forename: Richard
authors.initials: R
authors.lastname: Thompson
authors.forename: Dean
authors.initials: D
authors.lastname: Ellison
authors.forename: Jean M
authors.initials: JM
authors.lastname: Wyvratt
mesh_terms: D000097:Acetonitriles; D019075:Electrophoresis, Capillary / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry* / Q000302:isolation & purification*; D007202:Indicators and Reagents; D015394:Molecular Structure; D012996:Solutions; D013237:Stereoisomerism; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000097:Acetonitriles; D004791:Enzyme Inhibitors; D007093:Imidazoles; D007202:Indicators and Reagents; D012996:Solutions; D047392:beta-Cyclodextrins; D048051:p38 Mitogen-Activated Protein Kinases; C031215:betadex; C032159:acetonitrile