Multiplex PCR method for identifying recombinant vaccine-related polioviruses.
The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.
Kilpatrick
David R
DR
Respiratory and Enteric Virus Branch, G-10, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. DKilpatrick@cdc.gov
Ching
Karen
K
Iber
Jane
J
Campagnoli
Ray
R
Freeman
Christopher J
CJ
Mishrik
Nada
N
Liu
Hong-Mei
HM
Pallansch
Mark A
MA
Kew
Olen M
OM
D001483:Base Sequence; D017931:DNA Primers; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification*; D023321:Poliovirus Vaccines / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods*; D014614:Vaccines, Synthetic / Q000592:standards*
D017931:DNA Primers; D023321:Poliovirus Vaccines; D014614:Vaccines, Synthetic