title: Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29.
abstract: Protein myristoylation is a co-translational process, catalyzed by N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60(c-src) expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60(c-src), and NIP71 in HCCLs.
authors.affiliation: Department of Pathology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 4H4.
authors.forename: Ponniah
authors.initials: P
authors.lastname: Selvakumar
authors.forename: Ashakumary
authors.initials: A
authors.lastname: Lakshmikuttyamma
authors.forename: Zoe
authors.initials: Z
authors.lastname: Lawman
authors.forename: Keith
authors.initials: K
authors.lastname: Bonham
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Dimmock
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000626:Aminopeptidases / Q000235:genetics*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms; D004791:Enzyme Inhibitors / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*
chemical_list: D004791:Enzyme Inhibitors; C492718:N-myristoyltransferase inhibitor protein 71, human; D009363:Neoplasm Proteins; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase; D000626:Aminopeptidases; C106541:methionine aminopeptidase 2; D008666:Metalloendopeptidases