Tumor necrosis factor alpha antibody prevents brain damage of rats with acute necrotizing pancreatitis.
OBJECTIVE
To study the protective effects of tumor necrosis factor alpha (TNFalpha) antibody on pancreatic encephalopathy in rats.


METHODS
One hundred and twenty SD rats were randomly divided into normal control group, acute necrotizing pancreatitis group and TNFalpha antibody treated group. Acute hemorrhage necrotizing pancreatitis model in rats was induced by retrograde injection of 50 g/L sodium taurocholate into the pancreatobiliary duct. Serum TNFalpha was detected and animals were killed 12 h after drug administration. Changes in content of brain water, MDA and SOD as well as leucocyte adhesion of brain microvessels were measured.


RESULTS
In TNFalpha antibody treated group, serum TNFalpha level was decreased. Content of brain water, MDA and SOD as well as leucocyte adhesion were decreased significantly in comparison with those of acute necrotizing pancreatitis group (P<0.05).


CONCLUSIONS
TNFalpha antibody can alleviate the brain damage of rats with acute hemorrhage necrotizing pancreatitis.
Yang
Yan-Ling
YL
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Li
Ji-Peng
JP
Li
Kai-Zong
KZ
Dou
Ke-Feng
KF
D000818:Animals; D000906:Antibodies / Q000627:therapeutic use*; D001921:Brain / Q000276:immunology / Q000473:pathology*; D001925:Brain Damage, Chronic / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D007958:Leukocyte Count; D008315:Malondialdehyde / Q000032:analysis; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000139:chemically induced / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis; D013656:Taurocholic Acid; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D014867:Water / Q000032:analysis
D000906:Antibodies; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D008315:Malondialdehyde; D013656:Taurocholic Acid; D013482:Superoxide Dismutase