Structural close-related aromatic compounds have different effects on laccase activity and on lcc gene expression in the ligninolytic fungus Trametes sp. I-62.
Nine phenolic compounds (p-coumaric acid, ferulic acid, guaiacol, syringol, p-methoxyphenol, pyrocatechol, phloroglucinol, 3,5-dihydroxybenzoic acid, and syringaldazine) were tested for their ability to increase laccase production in the ligninolytic basidiomycete Trametes sp. I-62. All these compounds resulted in increases in laccase activity, with the highest levels being detected in the presence of p-coumaric acid (273-fold) and guaiacol (73-fold). The three laccase isozyme genes in this fungus lcc1, lcc2, and lcc3 are differentially expressed in the presence of some of these aromatics with total lcc transcript levels differing markedly depending on the aromatic compound tested. Guaiacol (the best inducer of lcc gene transcription) and p-coumaric acid selectively induced expression of lcc1 and lcc2; ferulic acid induced lcc3 expression, while 3,5-dihydroxybenzoic acid had no marked effect on laccase gene transcription. The results demonstrate that close-related aromatic compounds appear to have different effects on both laccase activity levels and lcc gene expression in this basidiomycete.
Terrón
María C
MC
Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, E-28040 Madrid, Spain.
González
Tania
T
Carbajo
José M
JM
Yagüe
Susana
S
Arana-Cuenca
Ainhoa
A
Téllez
Alejandro
A
Dobson
Alan D W
AD
González
Aldo E
AE
D003373:Coumaric Acids / Q000494:pharmacology; D020536:Enzyme Activators / Q000494:pharmacology; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D006139:Guaiacol / Q000494:pharmacology; D006841:Hydrocarbons, Aromatic / Q000494:pharmacology*; D062385:Hydroxybenzoates / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics; D042845:Laccase / Q000096:biosynthesis / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D020072:Polyporales / Q000201:enzymology* / Q000235:genetics; D011422:Propionates; D012331:RNA, Fungal / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012118:Resorcinols; D014158:Transcription, Genetic
D003373:Coumaric Acids; D020536:Enzyme Activators; D006841:Hydrocarbons, Aromatic; D062385:Hydroxybenzoates; D007527:Isoenzymes; D010636:Phenols; D011422:Propionates; D012331:RNA, Fungal; D012333:RNA, Messenger; D012118:Resorcinols; C076950:alpha-resorcylic acid; D006139:Guaiacol; C004999:ferulic acid; D042845:Laccase; C495469:p-coumaric acid