title: Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
abstract: Expression array data for >3000 individual clones from two suppression subtractive hybridization libraries revealed 147 genes overexpressed in non-small-cell lung cancer (NSCLC) cell lines. Of these 147 genes, 30 genes have previously unknown cancer association and 65 genes have been associated with cancers other than NSCLC. The identification of 52 genes previously associated with NSCLC by different methodologies supports the validity of the strategy used here. Of the 147 genes, 19 have no prior named Unigene cluster designation, and are designated herein as L1 to L19. Quantitative real-time PCR and cancer profiling arrays were used as independent validation tools to confirm tumor overexpression for five of the 'L' genes in tumor cell lines and patient samples from NSCLC and other cancers. Follow-up studies for candidate NSCLC-associated genes can be useful in providing valuable insight into the etiology of lung cancer as well as providing potentially interesting diagnostic or therapeutic targets for further investigation.
authors.affiliation: Department of Antibody Technologies, Seattle Genetics Inc., 21823 30th Drive SE, Bothell, WA 98021, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Petroziello
authors.forename: Andrew
authors.initials: A
authors.lastname: Yamane
authors.forename: Lori
authors.initials: L
authors.lastname: Westendorf
authors.forename: Melissa
authors.initials: M
authors.lastname: Thompson
authors.forename: Charlotte
authors.initials: C
authors.lastname: McDonagh
authors.forename: Charles
authors.initials: C
authors.lastname: Cerveny
authors.forename: Che-Leung
authors.initials: CL
authors.lastname: Law
authors.forename: Alan
authors.initials: A
authors.lastname: Wahl
authors.forename: Paul
authors.initials: P
authors.lastname: Carter
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000235:genetics; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000235:genetics*
chemical_list: D005819:Genetic Markers