The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis.
An ERF/AP2-type transcription factor (CaPF1) was isolated by differential-display reverse transcription-PCR, following inoculation of the soybean pustule pathogen Xanthomonas axonopodis pv glycines 8ra, which induces hypersensitive response in pepper (Capsicum annuum) leaves. CaPF1 mRNA was induced under conditions of biotic and abiotic stress. Higher levels of CaPF1 transcripts were observed in disease-resistant tissue compared with susceptible tissue. CaPF1 expression was additionally induced using various treatment regimes, including ethephon, methyl jasmonate, and cold stress. To determine the role of CaPF1 in plants, transgenic Arabidopsis and tobacco (Nicotiana tabacum) plants expressing higher levels of CaPF1 were generated. Gene expression analyses of transgenic Arabidopsis and tobacco revealed that the CaPF1 level in transgenic plants affects expression of genes that contain either a GCC or a CRT/DRE box in their promoter regions. Furthermore, transgenic Arabidopsis plants expressing CaPF1 displayed tolerance against freezing temperatures and enhanced resistance to Pseudomonas syringae pv tomato DC3000. Disease tolerance was additionally observed in CaPF1 transgenic tobacco plants. The results collectively indicate that CaPF1 is an ERF/AP2 transcription factor in hot pepper plants that may play dual roles in response to biotic and abiotic stress in plants.
Yi
So Young
SY
Plant Genomics Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejeon 305-600, Korea.
Kim
Jee-Hyub
JH
Joung
Young-Hee
YH
Lee
Sanghyeob
S
Kim
Woo-Taek
WT
Yu
Seung Hun
SH
Choi
Doil
D
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002212:Capsicum / Q000235:genetics* / Q000502:physiology*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005615:Freezing; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*
D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins; D014157:Transcription Factors