Isolation of diferulic bridges ester-linked to arabinan in sugar beet cell walls.
After degradation of sugar beet cell walls with Driselase and fractionation of the solubilised products by hydrophobic interaction chromatography, a dehydrodiferuloylated oligoarabinan was isolated. Its structure was assigned to two dimers of (1-->5)-linked arabinose units esterified by a central 8-O-4' ferulic dimer. These results provide the first direct evidence that pectic arabinans in sugar beet cell walls may be covalently cross-linked through dehydrodiferulates.
Levigne
Sébastien
S
Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, INRA, rue de la Géraudière, B.P. 71627, F-44316 Nantes Cedex 3, France.
Ralet
Marie-Christine
MC
Quéméner
Bernard
B
Thibault
Jean-François
JF
D027461:Beta vulgaris / Q000737:chemistry*; D002850:Chromatography, Gel; D003373:Coumaric Acids / Q000737:chemistry* / Q000302:isolation & purification; D019281:Dimerization; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization
D003373:Coumaric Acids; D007202:Indicators and Reagents; D009844:Oligosaccharides; D011134:Polysaccharides; C030080:araban; C004999:ferulic acid