Affinity-based screening techniques for enhancing lead discovery.
Contemporary, rational small-molecule lead discovery methods, comprising target identification, assay development, high-throughput screening (HTS), hit characterization and medicinal chemistry optimization, dominate early-stage drug discovery strategies in many pharmaceutical companies. There is a growing disparity between the increasing cost of funding these methods and the decreasing number of new drugs reaching the market. New strategies must be adopted to reverse this trend. The use of genomics- and proteomics-based target discovery efforts can aid the process by dramatically increasing the number of novel, more highly validated targets entering the discovery process, but HTS must meet this increased demand with faster, cheaper technologies. Although activity-based screening strategies are typically efficient, allowing one scientist to interrogate tens of thousands of compounds per day, affinity-based screening strategies can allow much greater efficiency in the overall process. Affinity-based methods can play a role in both facilitating the screening of a greater number of targets and in efficiently characterizing the primary hits discovered.
Comess
Kenneth M
KM
Abbott Laboratories, Global Pharmaceutical Research Division, 100 Abbott Park Road, Abbott Park, IL 60048, USA. kenneth.m.comess@abbott.com
Schurdak
Mark E
ME
D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000191:economics / Q000379:methods* / Q000639:trends; D013678:Technology, Pharmaceutical / Q000191:economics / Q000379:methods* / Q000639:trends