[Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar].
OBJECTIVE
To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.


METHODS
Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.


RESULTS
Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).


CONCLUSIONS
Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.
Li
Shi-rong
SR
Department of Plastic and Aesthetic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Liu
Jian-yi
JY
Ji
Shu-xing
SX
D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D055513:Connective Tissue Growth Factor; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; D003094:Collagen