Long-sarafotoxins: characterization of a new family of endothelin-like peptides.
Sarafotoxins (SRTXs) constitute a family of vasoactive peptides that were initially isolated from the venom of Atractaspis engaddensis, and that are structurally and functionally related to endothelins (ETs). Analysis of the venom of Atractaspis microlepidota microlepidota revealed several new SRTX molecules manifesting some new structural and functional characteristics. These novel SRTXs are longer by three amino acids than the previously described SRTXs, and are designated here "long-SRTXs". Six isoforms, derived from new poly-cistronic precursors, have been identified so far in the venom of this snake. One of these isoforms, designated SRTX-m, was chemically synthesized and its biological properties were studied. Our results show that SRTX-m induces toxicity in mice, mostly due to vasoconstriction, and also that it has a lower toxicity and potency than the more potent SRTX described up to now: sarafotoxin-b (SRTX-b) from A. engaddensis.
Hayashi
Mirian A F
MA
Biochemical and Biophysical Laboratory, Instituto Butantan, SP 05503-900 Sao Paulo, Brazil.
Ligny-Lemaire
Caroline
C
Wollberg
Zvi
Z
Wery
Michaël
M
Galat
Andrzej
A
Ogawa
Tomohisa
T
Muller
Bruno H
BH
Lamthanh
Hung
H
Doljansky
Yvon
Y
Bdolah
Avner
A
Stöcklin
Reto
R
Ducancel
Frédéric
F
D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000737:chemistry* / Q000633:toxicity; D006339:Heart Rate / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000633:toxicity; D011817:Rabbits; D012911:Snakes; D014662:Vasoconstrictor Agents / Q000737:chemistry / Q000633:toxicity; D014757:Viper Venoms / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity
D016232:Endothelins; D010455:Peptides; D020033:Protein Isoforms; D014662:Vasoconstrictor Agents; D014757:Viper Venoms; C057354:sarafotoxins s6