title: Effect of a nutritional intervention promoting the Mediterranean food pattern on electrophoretic characteristics of low-density lipoprotein particles in healthy women from the Québec City metropolitan area.
abstract: The objective of the present study was to evaluate the effect of a nutritional intervention promoting the Mediterranean food pattern in free-living conditions on LDL electrophoretic characteristics in a group of seventy-one healthy women, aged between 30 and 65 years. The 12-week nutritional intervention consisted of two courses on nutrition and seven individual sessions with a dietitian. The first course provided information on the Mediterranean food pattern and the second was a cooking lesson. LDL peak particle diameter (LDL-PPD) and cholesterol levels in small (LDL-cholesterol<255 A) and large LDL fractions (LDL-cholesterol>260 A) were obtained by 2-16 % polyacrylamide gel electrophoresis of whole plasma. The sample was divided on the basis of baseline LDL-PPD using tertiles of the distribution (258.4 A and 260.0 A). Among the total sample of women, no significant change in LDL-PPD was observed in response to the nutritional intervention. However, subjects who at baseline were in the first tertile of the LDL-PPD distribution (<258.4 A) showed a significant increase in LDL-PPD and in the proportion of LDL %>260 A in response to the 12-week nutritional intervention (P<0.05). In contrast, LDL-PPD decreased significantly (P=0.007) among women with large LDL particles at baseline (LDL-PPD >260 A) while the proportion of LDL %<255 A and of LDL %>260 A remained unchanged. To conclude, changes in the food pattern, in response to a nutritional intervention promoting the Mediterranean food pattern, were accompanied by beneficial modifications in LDL electrophoretic characteristics in women who were characterised at baseline by smaller LDL particles.
authors.affiliation: Institute of Nutraceuticals and Functional Foods, 2440 Hochelaga Blvd, Laval University, Québec, Canada, G1K 7P4.
authors.forename: Julie
authors.initials: J
authors.lastname: Goulet
authors.forename: Benoît
authors.initials: B
authors.lastname: Lamarche
authors.forename: Amélie
authors.initials: A
authors.lastname: Charest
authors.forename: Geneviève
authors.initials: G
authors.lastname: Nadeau
authors.forename: Annie
authors.initials: A
authors.lastname: Lapointe
authors.forename: Sophie
authors.initials: S
authors.lastname: Desroches
authors.forename: Simone
authors.initials: S
authors.lastname: Lemieux
mesh_terms: D000328:Adult; D000368:Aged; D001055:Apolipoproteins B / Q000097:blood; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D038441:Diet, Mediterranean; D004435:Eating / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008875:Middle Aged; D010316:Particle Size
chemical_list: D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL