Cytotoxic constituents of Diadema setosum.
5,8-Epidioxycholest-6-en-3-ol (1), cholesterol (2), glycerol 1-palmitate (3) and glycerol 1,3-dioleate-2-stearate (4) were isolated from the methanol extract of the sea urchin Diadema setosum, which was collected from the Halong sea, Vietnam. Chemical structures were established based on extensive 1D, 2D-NMR, FAB-MS, El-MS spectroscopic data and GC-MS analysis. The NMR spectral data of compound 1 were reassigned by using HMQC and HMBC. Compound 1 was found to have strong cytotoxic effect against various cancer cell lines, such as KB (IC50, 2.0 microg/mL), FL (ICso, 3.93 microg/mL), and Hep-2 (IC50, 2.4 microg/mL) by in vitro assay.
Minh
Chau Van
CV
Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam.
Kiem
Phan Van
PV
Huong
Le Mai
le M
Kim
Young Ho
YH
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012617:Sea Urchins / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents