title: Expression of hemokinin 1 mRNA by murine dendritic cells.
abstract: Hemokinin 1 is encoded by preprotachykinin C (PPT-C) mRNA, and has been proposed as a regulator of B and T cell lymphopoiesis. Here we demonstrate the expression of mouse PPT-C mRNA by CD11b+ macrophages, CD11c+ dendritic cells and in the microglial cell line EOC 13.31. Expression was detected in freshly isolated CD11b+CD11c+ bone marrow cells, as well as in M-CSF expanded bone marrow-derived macrophages and GM-CSF expanded bone marrow-derived dendritic cells. There was preferential expression of PPT-C mRNA in dendritic cell subpopulations that were CD11b+, but not B220+ or GR-1+. These studies are the first to demonstrate PPT-C mRNA expression by cells of the myeloid lineage.
authors.affiliation: Department of Biology, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, NC 28223, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Nelson
authors.forename: Ian
authors.initials: I
authors.lastname: Marriott
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Bost
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D018845:CD11 Antigens / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D022423:Myeloid Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D038042:Myelopoiesis / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D015320:Tachykinins / Q000235:genetics*
chemical_list: D018845:CD11 Antigens; D011498:Protein Precursors; D012333:RNA, Messenger; C423233:Tac4 protein, mouse; D015320:Tachykinins; C044206:preprotachykinin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D017493:Leukocyte Common Antigens