Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells.
Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3-10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14-15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.
Ha
Moon Kyung
MK
Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
Chung
Kee Yang
KY
Lee
Ju Hee
JH
Bang
Dongsik
D
Park
Yoon Kee
YK
Lee
Kwang Hoon
KH
D000918:Antibody Specificity; D015415:Biomarkers; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D003429:Cross Reactions; D020405:Dermis / Q000098:blood supply / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D050556:Fatty Acid-Binding Proteins; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D015595:Skin Aging / Q000502:physiology*; D001616:beta-Galactosidase
D015415:Biomarkers; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D001616:beta-Galactosidase