title: Interaction between plasminogen activator inhibitor type-2 and pre-mRNA processing factor 8.
abstract: The plasminogen activator inhibitor type-2 (PAI-2) dependent apoptosis protection is due to the 33 amino acids fragment located between helix C and D of PAI-2, this fragment may interact with some unknown intracellular proteins. In this study we used the fragment between helix C and D of PAI-2 as a bait to perform a yeast two-hybrid screen using a cDNA library constructed with HeLa cells during apoptosis, and retrieved a clone encoding 94 amino acid residues of C-terminus of pre-mRNA processing factor 8 (PRPF8). Co-immunoprecipitation experiments confirmed that PAI-2 could interact with PRPF8 in vivo. PAI-2 could bind PRPF8 C-terminal in both the inside and outside of nuclear. These results suggested that the interaction between these two proteins might not be involved in the apoptosis process.
authors.affiliation: Department of Molecular Genetics, Shanghai Medical School of Fudan University, Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Chang
authors.initials: C
authors.lastname: Tong
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.forename: Yun-Song
authors.initials: YS
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002999:Clone Cells; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D017396:Plasminogen Activator Inhibitor 2 / Q000737:chemistry / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; C115374:PRPF8 protein, human; D017396:Plasminogen Activator Inhibitor 2; D012322:RNA Precursors; D016601:RNA-Binding Proteins