[Effect of S-2-(3-aminopropylamino) ethyl phosphorothioic acid on apoptosis and proliferation inhibition of HL-60 cell line].
To study the effects of S-2-(3-aminopropylamino) ethyl phosphorothioic acid (WR-2721, amifostine) on proliferation inhibition and apoptosis of HL-60 human leukemia cell line, the cell apoptosis rate of HL-60 was determined by annexin V/PI double staining method. Cell proliferation and chemotherapy sensitivity were analyzed with XTT assay, and the changes of cell cycle were observed through flow cytometry. The results showed that WR-2721 could significantly inhibit HL-60 cell proliferation. After treatment (30 min, 37 degrees C) with WR-2721, the sensitivity of HL-60 cells to VP16 was enhanced, and the IC(50) descended from 52.5 micro g/ml to 40.5 microg/ml. After 72 hours treatment of HL-60 cells with WR-2721, the early apoptotic cells (annexin V-FITC positive/PI negative) were increased from (5.5 +/- 1.9)% to (48.5 +/- 8.4)% (P < 0.001), late apoptotic cells (annexin V-FITC positive/PI positive) were increased from (1.2 +/- 0.5)% to (39.0 +/- 4.0)% (P < 0.001), and HL-60 cells were arrested in G(2)-M phase. In conclusion, WR-2721 treatment can enhance HL-60 cell chemotherapy sensitivity to VP16, inhibit proliferation, induce apoptosis and accumulation of cells in G(2)-M phase.
Chen
Bao-An
BA
Department of Hematology, The Affiliated Zhongda Hospital of East-south University, Nanjing 210009, China. cba8888@hotmail.com
Li
Cui-Ping
CP
Huang
Cheng-Yin
CY
Zhou
Min
M
Cheng
Xin
X
Chen
Jin
J
Fu
Qiang
Q
Gao
Feng
F
Gao
Chong
C
Ding
Jiao-Hua
JH
Sun
Geng-Yun
GY
Wang
Jun
J
Cheng
Jian
J
D004999:Amifostine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004357:Drug Synergism; D005047:Etoposide / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans
D005047:Etoposide; D004999:Amifostine