[Human anti-HCMV neutralizing Fab antibody generated by phage display library].
BACKGROUND
Cloning recombinant human Fab fragment against HCMV for the purpose of prophylaxis and control of HCMV infection.


METHODS
The authors constructed a HCMV phage display library with 2 x 10(6) clones, then used purified HCMV viral lysates to pan the library, then screened by ELISA.


RESULTS
Three clones showed positive responses in ELISA, they also showed high specificity in IFA, two of them could neutralize HCMV in neutralizing assays.


CONCLUSIONS
The specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. These results provide us the basis for further research of neutralizing recombinant human whole IgG molecule.
Duan
Tao
T
Virus Emergency Biotech Center, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Liang
Mi-fang
MF
Gu
Shu-yan
SY
D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology*; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000821:virology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D019151:Peptide Library
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library