In situ hybridization: detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples.
In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.
Mabruk
Mohamed J E M F
MJ
Advanced Medical and Dental Institute, University Sains Malaysia, Eureka Complex, 11800 USM, Pulau Penang, Malaysia. mmabruk03@yahoo.co.uk
D004279:DNA, Viral / Q000032:analysis*; D005557:Formaldehyde / Q000737:chemistry*; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods* / Q000639:trends; D015341:Nucleic Acid Probes / Q000032:analysis; D016612:Paraffin Embedding; D012367:RNA, Viral / Q000032:analysis
D004279:DNA, Viral; D015341:Nucleic Acid Probes; D012367:RNA, Viral; D005557:Formaldehyde