A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives.
A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved.
Xie
Jianping
J
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Chen
Xiaofeng
X
Zhang
Jiyou
J
Liu
Jiaqin
J
Tian
Jianniao
J
Chen
Xingguo
X
Hu
Zhide
Z
D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007834:Lasers; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D009327:4-Chloro-7-nitrobenzofurazan