Problems in monitoring horizontal gene transfer in field trials of transgenic plants.
Transgenic crops are approved for release in some countries, while many more countries are wrestling with the issue of how to conduct risk assessments. Controls on field trials often include monitoring of horizontal gene transfer (HGT) from crops to surrounding soil microorganisms. Our analysis of antibiotic-resistant bacteria and of the sensitivity of current techniques for monitoring HGT from transgenic plants to soil microorganisms has two major implications for field trial assessments of transgenic crops: first, HGT from transgenic plants to microbes could still have an environmental impact at a frequency approximately a trillion times lower than the current risk assessment literature estimates the frequency to be; and second, current methods of environmental sampling to capture genes or traits in a recombinant are too insensitive for monitoring evolution by HGT. A model for HGT involving iterative short-patch events explains how HGT can occur at high frequencies but be detected at extremely low frequencies.
Heinemann
Jack A
JA
New Zealand Institute of Gene Ecology, University of Canterbury, 8020, Private Bag 4800, Christchurch, New Zealand. jack.heinemann@canterbury.ac.nz
Traavik
Terje
T
D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005820:Genetic Testing / Q000379:methods; D030821:Plants, Genetically Modified / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012107:Research Design; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D012988:Soil Microbiology
D011994:Recombinant Proteins