title: Prevalence of the K469E polymorphism of intercellular adhesion molecule 1 gene in Italian patients with inflammatory bowel disease.
abstract: BACKGROUND
  Intercellular adhesion molecule 1 plays an important role in the recruitment of leucocytes at sites of inflammation and is up-regulated in intestinal mucosa of inflammatory bowel disease. Intercellular adhesion molecule 1 gene lies on chromosome 19p13, implicated in determining susceptibility to inflammatory bowel disease. Recently, the polymorphism K469E of intercellular adhesion molecule 1 gene has been identified.
  
  
  OBJECTIVE
  To assess the potential association of this polymorphism with inflammatory bowel disease.
  
  
  METHODS
  A total of 165 inflammatory bowel disease patients, 75 with Crohn's disease and 90 with ulcerative colitis, and 187 controls were studied.
  
  
  METHODS
  The K469E polymorphism was detected by polymerase chain reaction and restriction enzyme analysis. Statistical analysis was performed by chi2-test.
  
  
  RESULTS
  In inflammatory bowel disease, the distribution of intercellular adhesion molecule 1 genotypes was 24.9% E/E, 44.2% E/K and 30.9% K/K. In controls, 11.8% showed E/E genotype, 55.6% E/K and 32.6% K/K. The frequency of the E/E genotype was significantly higher in inflammatory bowel disease (Crohn's disease and ulcerative colitis) patients than in controls. Subgroup analysis showed that the frequency of the E469 allele was significantly increased only in Crohn's disease patients with ileocolonic location of disease and penetrating behaviour compared with controls.
  
  
  CONCLUSIONS
  We found an association of inflammatory bowel disease with the E/E genotype of intercellular adhesion molecule 1 gene, while allele E469 was associated with a subgroup of Crohn's disease patients with more extensive location of disease and penetrating behaviour. However, further studies are needed to confirm our findings.
authors.affiliation: Department of Internal Medicine, Gastroenterology Unit, Catholic University of Rome, A. Gemelli University Hospital, Largo A. Gemelli 8, 00168 Rome, Italy. apapa@rm.unicatt.it
authors.forename: A
authors.initials: A
authors.lastname: Papa
authors.forename: R
authors.initials: R
authors.lastname: Pola
authors.forename: A
authors.initials: A
authors.lastname: Flex
authors.forename: S
authors.initials: S
authors.lastname: Danese
authors.forename: A
authors.initials: A
authors.lastname: Armuzzi
authors.forename: E
authors.initials: E
authors.lastname: Gaetani
authors.forename: L
authors.initials: L
authors.lastname: Guidi
authors.forename: I
authors.initials: I
authors.lastname: De Vitis
authors.forename: R
authors.initials: R
authors.lastname: Urgesi
authors.forename: A
authors.initials: A
authors.lastname: Grillo
authors.forename: M
authors.initials: M
authors.lastname: Serricchio
authors.forename: A S
authors.initials: AS
authors.lastname: Proia
authors.forename: G
authors.initials: G
authors.lastname: Fedeli
authors.forename: G
authors.initials: G
authors.lastname: Gasbarrini
authors.forename: P
authors.initials: P
authors.lastname: Pola
authors.forename: A
authors.initials: A
authors.lastname: Gasbarrini
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000235:genetics; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D007558:Italy; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D015995:Prevalence
chemical_list: D018799:Intercellular Adhesion Molecule-1