Electrophoresis in organogels.
A new matrix for electrophoresis, a low molecular weight organogel, is described. Dansylated amino acids and peptides were separated by planar and capillary electrophoresis in acetonitrile gels of trans-(1S,2S)-1,2-bis(dodecylamido)cyclohexane. The superior separation ability of the organogel over its corresponding buffer solution in capillary electrophoresis is illustrated. Organogels provide all the advantages associated with planar matrixes with 100% efficient recovery and transfer of the analytes to a mass spectrometer. We demonstrate that the planar gel can be liquefied and injected as is into an ESI-MS to identify the separands.
Mizrahi
Shaul
S
Casali Institute of Applied Chemistry, Chemistry Institute, Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Gun
Jenny
J
Kipervaser
Z Gidon
ZG
Lev
Ovadia
O