Characterization of Leishmania parasites isolated from provinces of the Islamic Republic of Iran.
Leishmania parasites isolated in the Islamic of Iran were studied by a random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Of 82 isolates, 80 were from cutaneous lesions, 1 from a human throat lesion and 1 from a dog. Of these, 42 isolates were L. tropica, 36 were L. major and 2 were L. infantum. There were 2 unidentified isolates (from the throat lesion and a cutaneous lesion) and these demonstrated 52% and 48% similarity with L. tropica and L. infantum. Both L. tropica and L. major were isolated from four provinces indicating a recent change in the epidemiology of cutaneous leishmaniasis. L. tropica was isolated from three provinces; L. major from one province. L. infantum was isolated from a human cutaneous lesion and from a dog in Bushehr province.
Motazedian
H
H
Department of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
Noamanpoor
B
B
Ardehali
S
S
D000818:Animals; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004197:Disease Reservoirs / Q000706:statistics & numerical data / Q000662:veterinary; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D019353:Endemic Diseases / Q000706:statistics & numerical data; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007492:Iran / Q000453:epidemiology; D018314:Leishmania infantum / Q000235:genetics / Q000302:isolation & purification; D018320:Leishmania major / Q000235:genetics / Q000302:isolation & purification; D007895:Leishmania tropica / Q000235:genetics / Q000302:isolation & purification; D007896:Leishmaniasis / Q000453:epidemiology* / Q000469:parasitology* / Q000517:prevention & control / Q000662:veterinary; D018579:Patient Selection; D011159:Population Surveillance; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012111:Residence Characteristics / Q000706:statistics & numerical data
D000981:Antiprotozoal Agents; D016054:DNA, Protozoan