title: An ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (MEHP) on testes in Shiba goat in vitro.
abstract: In this study, the effects of mono(2-ethylhexyl) phthalate (MEHP), one of metabolites of di(2-ethylhexyl) phthalate, on immature Shiba goat testes in vitro were examined. The testes of 2-month-old Shiba goats were cut into smaller pieces, and seeded in medium. At 1, 3, 6 and 9 hr after administration of MEHP at various concentrations (0, 100 nmol ml(-1), 1 nmol ml(-1), and 1 x 10(-3) nmol ml(-1), respectively), the specimens were obtained for light and transmission electron microscopic observations. As a result, at 1 hr after exposure to MEHP, the vacuolization and nuclear membrane rupture appeared in Sertoli cells. Such alterations tended to gradually increase in number in time and dose-dependent manners. Moreover, by MEHP treatment, apoptotic spermatogenic cells (characterized with chromatin condensation, cytoplasm shrinkage without membrane rupture, still functioning cell organelles, and packed cell contents in membrane-bounded bodies), apoptotic Sertoli cells (characterized with nuclear membrane lysis, nuclear condensation), necrotic spermatogenic cells (characterized with swollen and ruptured mitochondria, plasma membrane lysis, spilt cell contents, and chromatin clumps), and necrotic Sertoli cells (characterized with marginated chromatins along the nuclear membrane, ruptured vesicles within the MNB, some swollen and ruptured cell organelles, e.g. mitochondria) could be identified. Conclusively, ultrastructurally the treatment with MEHP at low concentration tends to lead spermatogenic and Sertoli cells to apoptosis, whereas that at high concentration tends to lead spermatogenic and Sertoli cells to necrosis. Thus, the testicular tissue culture is advantageous for screening testicular toxicity of chemicals.
authors.affiliation: Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-865, Japan. andrebbo4@yahoo.com
authors.forename: Bibin Bintang
authors.initials: BB
authors.lastname: Andriana
authors.forename: Tat Wei
authors.initials: TW
authors.lastname: Tay
authors.forename: Ishii
authors.initials: I
authors.lastname: Maki
authors.forename: Mohammad Abdul
authors.initials: MA
authors.lastname: Awal
authors.forename: Yoshiakira
authors.initials: Y
authors.lastname: Kanai
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kurohmaru
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity*; D015511:Goat Diseases / Q000139:chemically induced* / Q000473:pathology; D006041:Goats; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009336:Necrosis; D012708:Sertoli Cells / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013733:Testicular Diseases / Q000139:chemically induced* / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D014617:Vacuoles / Q000502:physiology / Q000648:ultrastructure
chemical_list: D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate