[Cloning and analyzing of the cDNA sequence of CHS-A gene of Narcissus].
Chalcone synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids. The production of flower pigment is specifically regulated by the activity of CHS. We cloned the cDNA sequence of CHS-A gene from Narcissus by PCR and analyzed the coding sequence of gene. The result demonstrated that the sequence of the coding region was 1167bp, encoding a protein of 389 amino acid which was more than 80% homology with CHS of the other 8 plants, such as Nicotine abacus and Solana tuberosum.
Huang
Yin Yi
YY
The Key Laboratory for Cell Biology and Tumor Cell Engineering, Ministry of Education, Xiamen University, Xiamen 361005.
Shen
Ming Shan
MS
Chen
Liang
L
Li
Peng
P
Chen
Mu Zhuan
MZ
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D031427:Narcissus / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology
D018076:DNA, Complementary; D000217:Acyltransferases; C020141:flavanone synthetase