title: Molecular polymerase chain reaction diagnosis of pulmonary mucormycosis caused by Cunninghamella bertholletiae.
abstract: OBJECTIVE
  Mucormycosis is an uncommon but frequently fatal infection caused by strains of mucorales in immunocompromised hosts. In this study, we report a case of pulmonary mucormycosis associated with acute lymphocytic leukaemia caused by Cunninghamella bertholletiae, a rare pathogen.
  
  
  METHODS
  Retrospective analysis of the stored serum, sputum, and necropsy lung tissue samples from this patient, enabled subspecies identification by means of panfungal polymerase chain reaction (PCR), direct DNA sequencing of the PCR products, and homology search with nucleotide basic local alignment search tool.
  
  
  RESULTS
  The development of a reliable diagnostic blood test for angio-invasive fungal infections such as mucormycosis is desirable, because the sensitivity of culture for these fungi is extremely low.
  
  
  CONCLUSIONS
  Panfungal PCR on serial serum samples might be useful for the diagnosis of pulmonary mucormycosis.
authors.affiliation: Third Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi, Japan. kmakoto@kochi-ms.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Kazuto
authors.initials: K
authors.lastname: Togitani
authors.forename: Hisanori
authors.initials: H
authors.lastname: Machida
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Uemura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Hirokuni
authors.initials: H
authors.lastname: Taguchi
mesh_terms: D020093:Cunninghamella; D004271:DNA, Fungal / Q000235:genetics; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008172:Lung Diseases, Fungal / Q000150:complications / Q000175:diagnosis*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009091:Mucormycosis / Q000150:complications / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications*; D012189:Retrospective Studies; D016415:Sequence Alignment
chemical_list: D004271:DNA, Fungal