title: Pre-treatment with ozonized oxygen (O3) aggravates inflammation in septic rats.
abstract: OBJECTIVE
  Ozone is produced by neutrophils during bacterial killing. Its application was found to be beneficial in peritonitis patients. Therefore, we measured survival and cytokines after ozone pre-treatment in septic rats.
  
  
  METHODS
  With approval, 40 male Wistar-rats were allocated to 1) ozone pre-treatment for five days before intra-abdominal sepsis, or 2) no pre-treatment.
  
  
  METHODS
  The primary endpoint was mortality at 120 h. Secondary endpoints were plasma cytokine levels.
  
  
  RESULTS
  In the control group mortality was 50% (10/20 rats). After ozone pre-treatment, survival was only 35% (7/20 rats, Log-Rank test: P = 0.10). Ozone increased TNF-alpha and MIP-2 after infection: 127 +/- 23 pg/ml and 94 +/- 19 pg/ml (control group: 398 pg/ml and 369 pg/ml; P < 0.002 and P < 0.01). IL-6 levels were similar in both groups.
  
  
  CONCLUSIONS
  Ozone pre-treatment was pro-inflammatory in sepsis with a trend to reduced survival. Therefore, its effects in sepsis should be further evaluated in animal trials.
authors.affiliation: Department of Anaesthesia and Critical Care, University of Marburg, Baldingerstrasse 1, 35033, Marburg, Germany. torossia@med.uni-marburg.de.
authors.forename: A
authors.initials: A
authors.lastname: Torossian
authors.forename: S
authors.initials: S
authors.lastname: Ruehlmann
authors.forename: L
authors.initials: L
authors.lastname: Eberhart
authors.forename: M
authors.initials: M
authors.lastname: Middeke
authors.forename: H
authors.initials: H
authors.lastname: Wulf
authors.forename: A
authors.initials: A
authors.lastname: Bauhofer
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced* / Q000150:complications* / Q000473:pathology; D007267:Injections; D008297:Male; D010126:Ozone / Q000008:administration & dosage* / Q000494:pharmacology*; D011292:Premedication; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications* / Q000188:drug therapy / Q000473:pathology*; D016019:Survival Analysis
chemical_list: D010126:Ozone