title: In vitro host response assessment of biomaterials for cardiovascular stent manufacture.
abstract: The deployment of a vascular stent during angioplasty has greatly reduced the risks of restenosis. However, the presence of the device still induces a host response as well as a mechanical action on the blood vessel wall and an alteration of the haemodynamics. Platelet and inflammatory cells can adhere on the stent surface and be activated to produce biochemical signals able to stimulate an excessive proliferation of the smooth muscle cells with the consequent obstruction of the vessel lumen. For these reasons, the host response to two of the materials used in stent manufacture, stainless steel and diamond-like carbon, was investigated in vitro. The data showed that stainless steel induced a higher level of host response both in terms of platelet aggregation and macrophage activation. However, the spreading of inflammatory cells was more accentuated on diamond-like carbon. The inflammatory cells produced levels of platelet-derived growth factor, a key signal in smooth muscle cell proliferation, similar to stainless steel thus suggesting that carbon coatings may not be able to prevent restenosis.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Cockcroft Building, Lewes Road, Brighton BN2 4GJ, UK. m.santin@brighton.ac.uk
authors.forename: Matteo
authors.initials: M
authors.lastname: Santin
authors.forename: Lyuba
authors.initials: L
authors.lastname: Mikhalovska
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Lloyd
authors.forename: Sergey
authors.initials: S
authors.lastname: Mikhalovsky
authors.forename: Louise
authors.initials: L
authors.lastname: Sigfrid
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Denyer
authors.forename: Susanna
authors.initials: S
authors.lastname: Field
authors.forename: Dennis
authors.initials: D
authors.lastname: Teer
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D002478:Cells, Cultured; D018130:Diamond / Q000737:chemistry*; D005549:Foreign-Body Reaction / Q000209:etiology* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000276:immunology; D008422:Materials Testing / Q000379:methods*; D010973:Platelet Adhesiveness / Q000276:immunology; D010974:Platelet Aggregation / Q000276:immunology; D013193:Stainless Steel / Q000737:chemistry*; D015607:Stents / Q000009:adverse effects*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D013193:Stainless Steel; D018130:Diamond