title: [Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus].
abstract: BACKGROUND
  To study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).
  
  
  METHODS
  Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.
  
  
  RESULTS
  The inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies.
  
  
  CONCLUSIONS
  The ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.
authors.affiliation: Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Yan
authors.forename: Gao-sheng
authors.initials: GS
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Ji-liang
authors.initials: JL
authors.lastname: Feng
authors.forename: Guo-rong
authors.initials: GR
authors.lastname: Yang
mesh_terms: D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000737:chemistry* / Q000276:immunology / Q000821:virology; D042282:Microdissection; D012469:Salivary Glands / Q000737:chemistry / Q000276:immunology / Q000821:virology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral