Expression and characterization of cholera toxin B-pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTB-PsaA to induce humoral immune response in mice.
Cholera toxin B subunit (CTB) is responsible for CT holotoxin binding to the cell and has been described as a mucosal adjuvant for vaccines. In this work, the ctxB gene was genetically fused to the psaA gene from Streptococcus pneumoniae, a surface protein involved in its colonization in the host that is also considered a vaccine antigen candidate against this pathogen. The CTB-PsaA fusion protein was expressed in Escherichia coli, and the purified protein was used for intranasal immunization experiments in Balb/C mice. CTB-PsaA was able to induce both systemic and mucosal antibodies evaluated in serum, saliva, and in nasal and bronchial wash samples, showing that CTB-PsaA is a promising molecule to be investigated as S. pneumoniae vaccine antigen candidate.
Arêas
Ana Paula Mattos
AP
Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
Oliveira
Maria Leonor Sarno
ML
Miyaji
Eliane Namie
EN
Leite
Luciana Cezar Cerqueira
LC
Aires
Karina Araújo
KA
Dias
Waldely Oliveira
WO
Ho
Paulo Lee
PL
D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000942:Antigens, Bacterial / Q000235:genetics / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology*; D002772:Cholera Toxin / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; C051592:pneumococcal surface protein A; D002772:Cholera Toxin