title: Optimal timing to repopulation of resident alveolar macrophages with donor cells following total body irradiation and bone marrow transplantation in mice.
abstract: To determine the time required to repopulate mouse lungs with donor alveolar macrophages following total body irradiation (TBI) and bone marrow transplantation (BMT), C57Bl/6 mice were subjected to TBI with 900 cGy, followed by transplantation of bone marrow cells from mice expressing green fluorescent protein (GFP) in their somatic cells. The mice were euthanized at either 30 (n=5), 60 (n=5) or 90 (n=5) days following BMT. Thirty days following transplantation, 87.8 +/- 3.9% (mean +/- S.E.M.) circulating leukocytes in recipient mice were derived from the donor, as determined by fluorescence activated cell sorting (FACS) analysis for GFP. However, only 46.9 +/- 7.4% of the resident alveolar cells expressed GFP, indicating incomplete repopulation. By day 60 post-transplantation, the percentage of bronchoalveolar lavage fluid (BALF) cells expressing GFP reached 74.5 +/- 2.4%, remaining stable 90 days after transplantation (80.4 +/- 1.9%). We conclude that 60 days after TBI with 900 cGy and bone marrow transplantation, the majority of the lung resident alveolar macrophages is of donor origin. This study provides useful information regarding the time of reconstitution with donor alveolar macrophages in the pulmonary airspaces of recipient mice following marrow transplantation.
authors.affiliation: Pulmonary and Critical Care Division, Department of Medicine, University of Washington, Seattle, WA 98108, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Matute-Bello
authors.forename: Janet S
authors.initials: JS
authors.lastname: Lee
authors.forename: Charles W
authors.initials: CW
authors.lastname: Frevert
authors.forename: W Conrad
authors.initials: WC
authors.lastname: Liles
authors.forename: Steven
authors.initials: S
authors.lastname: Sutlief
authors.forename: Kimberly
authors.initials: K
authors.lastname: Ballman
authors.forename: Venus
authors.initials: V
authors.lastname: Wong
authors.forename: Amy
authors.initials: A
authors.lastname: Selk
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Martin
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D016026:Bone Marrow Transplantation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins