title: Isolation of Citrobacter sp. mutants defective in decolorization of brilliant green by transposon mutagenesis.
abstract: To identify genes involved in the decolorization of brilliant green, we isolated random mutants generated by transposon insertion in brilliant green-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 19 mutants with a complete defect in terms of the brilliant green color removing ability. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 7 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. By comparing these with a sequence database, putative protein products encoded by bg genes were identified as follows: bg 3 as a LysR-type regulatory protein; bg 11 as a MalG protein in the maltose transport system; bg 14 as an oxidoreductase; and bg 17 as an ABC transporter. The sequences deduced from the three bg genes, bg 2, bg 7 and bg 16, showed no significant similarity to any protein with a known function, suggesting that these three bg genes may encode unidentified proteins responsible for the decolorization of brilliant green.
authors.affiliation: Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
authors.forename: Moon-Sun
authors.initials: MS
authors.lastname: Jang
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Yong-Lark
authors.initials: YL
authors.lastname: Choi
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Cho
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D002954:Citrobacter / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D017353:Gene Deletion; D016254:Mutagenesis, Insertional; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014157:Transcription Factors / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C079123:MalG protein, E coli; D000644:Quaternary Ammonium Compounds; D014157:Transcription Factors; C073470:LysR protein, Bacteria; D010088:Oxidoreductases; C006798:brilliant green