[Effects of simulated microgravity on preimplantation embryonic development of Kunming mouse in vitro].
In order to investigate the development of Kunming mouse preimplantation embryos cultured in vitro under simulated microgravtiy, one-cell and 4-cell embryos of Kunming mouse (Fertilization In Situ) were cultured in CZB or KSOM media under simulated microgravity or normal gravity environment respectively. The results showed that under normal gravity, the percentage of passing 2-cell development block embryos was not different in CZB with in KSOM, but the percentage of blastocysts was lower in CZB than that in KSOM significantly. The percentages of passing 2-cell development block embryos and blastocysts in CZB or KSOM were lower under simulated microgravity than those under normal gravity. It is suggested that the frequency of early embryonic lethality is possibly increased by simulated microgravity.
Zhang
Shou Quan
SQ
College of Animal Science, South China Agricultural University, Guangzhou 510642.
Gao
Shu Jing
SJ
Jiang
Qin Yan
QY
Feng
Ding Yuan
DY
Pen
Zhi Yong
ZY
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D051379:Mice; D011247:Pregnancy; D014893:Weightlessness / Q000009:adverse effects*