title: Tumor neoangiogenesis by CD31 and CD105 expression evaluation in breast carcinoma tissue microarrays.
abstract: OBJECTIVE
  The aim of the study was to evaluate CD31 and CD105 immunohistochemical expressions in tissue microarrays from 360 breast carcinomas.
  
  
  METHODS
  Computerized (ACIS/Chromavision) assisted image analysis was performed to compare immunoreactions in tissue microarrays with those in current paraffin and frozen sections. We also aimed to determine the CD105 and CD31 prognostic significance and relevance in routine practice by correlating results of immunodetections with patients' (n = 360) outcome (14.3-year follow-up).
  
  
  RESULTS
  The results show (a) that in tissue microarrays, the CD31 and CD105 expression quantified by image analysis device did not correlate with the measurements assessed on routine paraffin sections; (b) that CD105 expression is endowed of a prognostic significance in paraffin sections in terms of overall survival (P < 0.01), whereas in contrast, CD31 on paraffin sections did not correlate with patients overall survival; (c) that semiquantitative analysis of CD105 expression correlated with the image analysis measurements in frozen sections (rho = 0.671, P < 0.01) and paraffin (rho = 0.824, P < 0.01) sections. However, paraffin sections were less immunostained than frozen ones.
  
  
  CONCLUSIONS
  It is concluded (a) that CD105 may be suitable in paraffin sections to evaluated neoangiogenesis; and (b) that tissue microarrays are not suitable substrates for neoangiogenesis evaluation as a prognostic indicator in breast carcinomas, in contrast to current tissue sections.
authors.affiliation: Department of Pathology, Centre Hospitalier, Universitaire Nord, France. colette.charpin@ao-hm.fr
authors.forename: Colette
authors.initials: C
authors.lastname: Charpin
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Dales
authors.forename: Stéphane
authors.initials: S
authors.lastname: Garcia
authors.forename: Séverine
authors.initials: S
authors.lastname: Carpentier
authors.forename: Amina
authors.initials: A
authors.lastname: Djemli
authors.forename: Lucile
authors.initials: L
authors.lastname: Andrac
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Lavaut
authors.forename: Claude
authors.initials: C
authors.lastname: Allasia
authors.forename: Pascal
authors.initials: P
authors.lastname: Bonnier
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply* / Q000175:diagnosis; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D015703:Antigens, CD; D001943:Breast Neoplasms / Q000098:blood supply* / Q000175:diagnosis / Q000378:metabolism; D000071063:Endoglin; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016612:Paraffin Embedding; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011379:Prognosis; D011956:Receptors, Cell Surface; D015996:Survival Rate; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1