Denaturing gradient gel electrophoresis analysis of bacterial populations in 5-stage biological nutrient removal process with step feed system for wastewater treatment.
Changes in the bacterial populations of a 5-stage biological nutrient removal (BNR) process, with a step feed system for wastewater treatment, were monitored by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA fragments. DGGE analysis indicated seasonal community changes were observed, however, community profiles of the total bacteria of each reactor showed only minor differences in the samples obtained from the same season. The number of major bands was higher in the summer samples, and decreased during the winter period, indicating that the microbial community structure became simpler at low temperatures. Since the nitrogen and phosphate removal efficiencies were highly maintained throughout the winter operation period, the bacteria which still remaining in the winter sample can be considered important, playing a key role in the present 5-stage BNR sludge. The prominent DGGE bands were excised, and sequenced to gain insight into the identities of the predominant bacterial populations present, and most were found to not be closely related to previously characterized bacteria. These data suggest the importance of culture-independent methods for the quality control of wastewater treatment.
Lee
Soo-Youn
SY
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Kim
Hyeon-Guk
HG
Park
Jong Bok
JB
Park
Yong Keun
YK
D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D011157:Population Dynamics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012621:Seasons; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid; D018508:Water Purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010710:Phosphates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009584:Nitrogen