title: Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.
abstract: We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
authors.affiliation: Unité de Génétique Moléculaire des Levures, URA2171 CNRS and UFR 927 Université Pierre et Marie Curie, Paris-Cedex 15, France. jboyer@pasteur.fr
authors.forename: Jeanne
authors.initials: J
authors.lastname: Boyer
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Badis
authors.forename: Cécile
authors.initials: C
authors.lastname: Fairhead
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Talla
authors.forename: Florence
authors.initials: F
authors.lastname: Hantraye
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Fabre
authors.forename: Gilles
authors.initials: G
authors.lastname: Fischer
authors.forename: Christophe
authors.initials: C
authors.lastname: Hennequin
authors.forename: Romain
authors.initials: R
authors.lastname: Koszul
authors.forename: Ingrid
authors.initials: I
authors.lastname: Lafontaine
authors.forename: Odile
authors.initials: O
authors.lastname: Ozier-Kalogeropoulos
authors.forename: Miria
authors.initials: M
authors.lastname: Ricchetti
authors.forename: Guy-Franck
authors.initials: GF
authors.lastname: Richard
authors.forename: Agnès
authors.initials: A
authors.lastname: Thierry
authors.forename: Bernard
authors.initials: B
authors.lastname: Dujon
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D005804:Genes, Lethal / Q000235:genetics; D016153:Genes, Suppressor; D016681:Genome, Fungal; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D016364:Reading Frames / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*; D014162:Transfection / Q000379:methods; D019076:Transgenes / Q000502:physiology
chemical_list: D004271:DNA, Fungal