[Effects of normal human bone marrow fibroblastoid stromal cell line on the proliferation of multiple myeloma cell line RPMI8226].
To investigate the effects of normal human bone marrow fibroblastoid stromal cell lines HFCL on the proliferation of multiple myeloma cell lines RPMI8226, the co-culture system of RPMI8226 with HFCL was established, the adhesion ratio was determined by MTT colorimetric assay, growth curves were determined by trypan blue exclusion, the mitotic index (MI) of RPMI8226 cell was observed by Wright-Giemsa staining. Flow cytometry and Western blot were used to study the changes of cell cycle and expression of proliferating cell nuclear antigen (PCNA), respectively. The results showed that in co-culture the adhesion ratio of RPMI8226 after 1 hour was 29.4%; the proliferation of RPMI8226 cell line in direct contact with HFCL cell line was inhibited as compared with RPMI8226 in suspension. No obvious changes were observed in RPMI8226 cell separated by transwell inserts. The percentage of G(1) phase cells of RPMI8226 cell line in direct contact with HFCL was higher than that of RPMI8226 in suspension, and the percentage of S phase cells was lower. Moreover, the MI of RPMI8226 cell line in suspension was higher than that of RPMI8226 cell line in direct contact with HFCL cell. The expression of PCNA in RPMI8226 cell line in suspension was higher than that of RPMI8226 cell in direct contact with HFCL cell. It is concluded that the normal human bone marrow fibroblastoid stromal cell HFCL inhibits the proliferation and progression of cell cycle of multiple myeloma cell line, RPMI8226.
Jin
Xue-Qin
XQ
Department of Hematology of Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
Bai
Qing-Xian
QX
Liang
Rong
R
Chen
Xie-Qun
XQ
Huang
Gao-Sheng
GS
Dong
Bao-Xia
BX
Zhang
Wei-Ping
WP
D001854:Bone Marrow Cells / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D018920:Coculture Techniques; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D017154:Stromal Cells / Q000502:physiology*
D018809:Proliferating Cell Nuclear Antigen