Self-contained on-chip cell culture and pretreatment system.
In this study, we describe a simple on-chip cell culture and pretreatment system that requires no external machines. Conventional cell culture utilizes culture dishes or microtiter plates, where pipetting and centrifugation are indispensable for washing cells and changing media. However, our microdevice requires no external centrifugation or pump. Utilizing this microdevice, we attained dramatically shorter total analytical time with a high-throughput screening system for proteomic analysis (1 min per 12 samples; one eightieth of the conventional time). Protein expression of Jurkat cells during stress-shock induced apoptosis was readily analyzed using this system. We found that a seaweed extraction effectively induced apoptosis of Jurkat cells.
Tabuchi
Mari
M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima COE, Tokushima 770-8505, Japan. tabuchi@ph.tokushima-u.ac.jp
Baba
Yoshinobu
Y
D017209:Apoptosis; D001681:Biological Assay; D018929:Cell Culture Techniques / Q000295:instrumentation*; D006801:Humans; D019169:Jurkat Cells; D008832:Microchemistry / Q000379:methods*; D008904:Miniaturization; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000737:chemistry*; D046888:Tissue Array Analysis
D011506:Proteins; D020543:Proteome