title: [Expression of cystic fibrosis transmembrane conductance regulator in human endometrium].
abstract: OBJECTIVE
  To study expression of cystic fibrosis transmembrane conductance regulator (CFTR) in human endometrium.
  
  
  METHODS
  The expression of CFTR mRNA and protein from 50 samples of normal cyclic human endometrium was examined by in situ hybridization, immunohistochemistry and Western blotting respectively.
  
  
  RESULTS
  CFTR mRNA and protein expressions were only detected in the endometrial glandular cells with cyclic changes. CFTR mRNA could be detected from the midproliferative phase with the highest level found in the late proliferative phase, significantly higher than those of late-secretory phase endometrium (P < 0.05). While a large quantity of CFTR protein were seen in late proliferative phase and still presented in the secretive and menstrual phases. Western blotting analysis demonstrated that human endometrium expressed the special CFTR band at 170 000.
  
  
  CONCLUSIONS
  The expression of CFTR in human endometrium presented in glandular cells and the amounts were varied cyclically. The abundant CFTR mRNAs and proteins around the ovulatory period may drive Na(+) and fluid from plasma into the uterine lumen to make optimal electrolyte composition and sufficient fluid volume for sperm migration.
authors.affiliation: Department of Obstetrics and Gynecology, Third Hospital, Peking University, Beijing 100083, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Zheng
authors.forename: Gui-An
authors.initials: GA
authors.lastname: Chen
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000328:Adult; D018118:Chloride Channels / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000235:genetics; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C505032:CFTR protein, human; D018118:Chloride Channels; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator