title: Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility.
abstract: We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.
authors.affiliation: Department of Biochemistry, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK.
authors.forename: Kirsty L
authors.initials: KL
authors.lastname: Lightbody
authors.forename: Philip S
authors.initials: PS
authors.lastname: Renshaw
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Collins
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Wright
authors.forename: Debbie M
authors.initials: DM
authors.lastname: Hunt
authors.forename: Stephen V
authors.initials: SV
authors.lastname: Gordon
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Roger S
authors.initials: RS
authors.lastname: Buxton
authors.forename: Richard A
authors.initials: RA
authors.lastname: Williamson
authors.forename: Mark D
authors.initials: MD
authors.lastname: Carr
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D046912:Multiprotein Complexes; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D046912:Multiprotein Complexes; D001616:beta-Galactosidase