title: Identification of a 98-kb DNA segment containing the rice Eui gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary.
abstract: The recessive 'tall rice' phenotype associated with the mutation eui (elongated upper-most internode) is an important agronomic trait that has been introduced into hybrid rice to eliminate panicle enclosure in all types of male-sterile lines and produce good-quality seeds in high yield and at low cost. Based on our previous Eui mapping data, we conducted fine-structure mapping and positional cloning of the gene using an F2 population comprising more than 5000 individuals derived from a cross of the near-isogenic lines 307T (eui/eui) with the recurrent parent Zhenshan 97 (Eui/Eui). In total 45 CAPS (cleaved amplified polymorphic sequences) markers located within an interval of 14.5 cM were analyzed in the subpopulation of 1298 homozygous recessive plants. The resulting high-resolution map defined a 98-kb interval containing the Eui locus flanked by the markers M0387 and M01, and three markers were found to co-segregate with Eui. In order to facilitate the identification of the Eui gene, we used a transformation-competent artificial chromosome (TAC) vector to construct a set of contiguous TAC clones from the Nipponbare BACs (obtained from the Clemson University Genome Institute; CUGI) spanning this region. These clones can be used to streamline complementation testing. The markers tightly linked to the Eui locus can also be used in breeding male-sterile lines with the elongated uppermost internode.
authors.affiliation: College of Life Sciences, Zhejiang University, 310029, Hangzhou, China.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Xu
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Zhu
authors.forename: H-C
authors.initials: HC
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: Z-X
authors.initials: ZX
authors.lastname: Sun
authors.forename: Y-G
authors.initials: YG
authors.lastname: Liu
authors.forename: H-X
authors.initials: HX
authors.lastname: Lin
authors.forename: Z-H
authors.initials: ZH
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005822:Genetic Vectors; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D030821:Plants, Genetically Modified
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers