[Cell origin of localized Castleman's disease of hyaline-vascular type].
OBJECTIVE
To investigate the cell features of 6 Castleman's disease patients and evaluate their prognosis.


METHODS
The resected tumors were investigated by routine histopathology and immunohistochemistry. Reverse-transcript PCR (RT-PCR) and sequencing of RT-PCR products were used to assess the clonal characters of the main tumor cells.


RESULTS
Histologically, all of the 6 tumors were classified as the hyaline vascular type. B-cells dominated the follicular germinal centers, with T-cells dispersing inter-follicularly. The results of RT-PCR each obtained a single band of either 128 bp or 122 bp and sequencing showed that there was highly homogeneity within the same length sequences, accompanied by fewer different nucleotide acids.


CONCLUSIONS
Monoclonal and/or oligoclonal B cells were identified in Castleman's disease. These B cells were originated from germinal center cells.
Wang
Liang-chun
LC
Department of Dermatology, Peking University First Hospital, Beijing 100034, China.
Bu
Ding-fang
DF
Zhu
Ping
P
Zhu
Xue-jun
XJ
D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000032:analysis; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D005871:Castleman Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002999:Clone Cells; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018951:Antigens, CD20; D017493:Leukocyte Common Antigens