title: [Construction of a genechip method for detection of hepatitis B virus DNA, basal core promotor and Pre-C mutants].
abstract: BACKGROUND
  To establish a genechip method for detection of hepatitis B virus (HBV) DNA, basal core promotor (BCP), and Pre-C mutants.
  
  
  METHODS
  This study used two kinds of technology (PCR, oligochip), which can detect five mutant hotspots including nt 1 896, nt 1 899, nt 1 862, nt 1 764 and nt 1 762. The results of genechip method was verified by DNA sequencing.
  
  
  RESULTS
  In detecting HBV DNA, the results of genechip were 100% consistent with those of DNA sequencing. In detecting HBV BCP and Pre-C mutants, 146 codons showed the same results using both methods, except for only 4 codons (P greater than 0.05).
  
  
  CONCLUSIONS
  This convenient high throughput genechip method could detect several BCP and Pre-C mutant codons at the same time. These results suggest that genechip method has the same positive rate and specificity with DNA sequencing method. It has more advantages than the latter in detecting mixed mutants and therefore may be used in clinical practice.
authors.affiliation: Shenzhen Eastlake Hospital, Shenzhen 518020, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Jin-fu
authors.initials: JF
authors.lastname: Peng
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Chen
authors.forename: Zhao-qin
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Li-xiong
authors.initials: LX
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Bo-ping
authors.initials: BP
authors.lastname: Zhou
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014758:Viral Core Proteins