title: In vitro control of fasciation in proliferating nucellar embryos of Mangifera indica L. var totapari red small for cloning.
abstract: Nucellar tissue contained in ovular halves of young fruits of Mangifera indica L. totapari red small, a dwarfing rootstock, differentiated fasciated embryonal structures in presence of 6-benzylaminopurine [BAP(0.15 mg l(-1))], 6-(gamma-gamma-dimethylallylamino) purine [2iP(0.15 mg l(-1))] and indole-3-acetic acid [(IAA(0.5 mg l(-1))] incorporated in the semisolid medium during 50-60 days. Due to embryonal fasciation, hardly 2-3 well-formed embryos could be obtained per culture of proliferating embryos. Of the 3 ethylene inhibitors [L-alpha-(2-aminoethoxyvinyl)-glycine-HCl (AVG), AgNO3 and salicylic acid (SA)] used, embryonal fasciation and necrosis of intervening tissue was completely controlled by 3-4 subcultures of fasciated mass of embryos under the influence of AVG (0.05 mg l(-1)) in presence of adenine sulphate [AdS (50 mg l(-1))] incorporated in the same medium. Almost synchronized development of isolated embryos, measuring ca 2 cm in length, was observed in a different medium used in liquid stationary state and supplemented, particularly with stress-producing substances [abscisic acid (ABA, 0.01 mg l(-1)); and polyethylene glycol (PEG, 100 mg l(-1))] besides certain other modifications. About 34% convertibility of processed embryos was obtained during a period of 90 days. The plantlets had well-developed roots along with laterals which were longer than leafy shoots. In vitro raised plants survived ex vitro for about 2 months.
authors.affiliation: Tissue Culture Laboratory, National Botanical Research Institute, Lucknow 226 001, India. hcc_nbri@hotmail.com
authors.forename: H C
authors.initials: HC
authors.lastname: Chaturvedi
authors.forename: S
authors.initials: S
authors.lastname: Agnihotri
authors.forename: M
authors.initials: M
authors.lastname: Sharma
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Jain
authors.forename: A
authors.initials: A
authors.lastname: Chourasia
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D005030:Ethylenes / Q000037:antagonists & inhibitors; D018525:Germination / Q000187:drug effects; D066298:In Vitro Techniques; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D031022:Mangifera / Q000187:drug effects* / Q000196:embryology* / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D005030:Ethylenes; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; C030737:indoleacetic acid; D000040:Abscisic Acid; C036216:ethylene; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin