[Study on Realgar inducing apoptosis in T lymphocytic cell line CEM].
OBJECTIVE
To study the Realgar induced T lymphocytic leukemia cell line CEM apoptosis in vitro.


METHODS
CEM cells were incubated with Realgar. Cell proliferation inhibition was determined by MTT. Cell cycle, apoptosis, Apo2.7 and Fas were measured by cytometer.


RESULTS
Realgar inhibited the proliferation of CEM cell line. The cells treated with Realgar showed a Sub-G(0)/G(1) apoptotic peak in DNA distribution histogram, increment of Apo2.7 protein expression, and arrested cells in G(2)/M phase, but ineffectiveness on Fas expression.


CONCLUSIONS
The Realgar can induce CEM cell apoptosis in vitro.
Zhang
Chen
C
TCM Hematology Institute of People's Liberation Army, Dalian, Liaoning Province 116021, China. zcshsmmu@sina.com
Huang
Shi-Lin
SL
Xiang
Yang
Y
Guo
Ai-Xia
AX
D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001152:Arsenicals / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology; D013440:Sulfides / Q000494:pharmacology*; D019014:fas Receptor / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D001152:Arsenicals; C491901:FAS protein, human; D013440:Sulfides; D019014:fas Receptor; C058317:arsenic disulfide