Attachment of DNA to the carbon fiber microelectrode via gold nanoparticles for simultaneous determination of dopamine and serotonin.
A novel biochemical sensor was fabricated on a carbon fiber microelectrode, which consisted of an inner layer of electrodeposited gold nanoparticles, as a nano-array electrode, and an outer layer of electrodeposited calf thymus ds-DNA at +1.5 V vs. SCE. This modified electrode was characterized by X-ray photoelectron spectroscopy, scanning electron microscope, atomic force microscopy, cyclic voltammetry and differential pulse voltammetry (DPV). It was found that this electrochemical sensor exhibits a strong catalytic activity toward the oxidation of dopamine (DA), serotonin (5-HT) and ascorbic acid (AA), as a result of resolving the anodic voltammetric peaks of DA, 5-HT and AA into three well-defined peaks. Simutaneous DPV determination of DA and 5-HT can be achieved in the presence of 2000-fold AA. The modified electrode shows good sensitivity, selectivity and stability.
Lu
Liping
L
Department of Chemistry, University of Science and Technology of China, Hefei, 230026, P R, China.
Wang
Shuqing
S
Lin
Xiangqin
X
D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D004247:DNA / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes; D036103:Nanotechnology / Q000379:methods*; D012701:Serotonin / Q000032:analysis* / Q000737:chemistry
D000077482:Carbon Fiber; D012701:Serotonin; D002244:Carbon; D006046:Gold; D004247:DNA; D001205:Ascorbic Acid; D004298:Dopamine