[Semen analysis of DNA fluorescence detection and two other routine analyzing methods].
OBJECTIVE
To compare semen analysis system of computer-aided sperm analysis (CASA) dyeing with DNA fluorescence (simplified as fluorescence CASA) with two other routine semen analyzing methods.


METHODS
Twenty-two male infertile patients were selected at random as research subjects, and semen analysis was performed with fluorescence CASA, grey CASA, and microscopic analysis.


RESULTS
Fluorescence CASA could distinguish sperm from non-sperm granules, as well as living from dead sperm. Compared with the sperm density detected by fluorescence CASA, the disparities of sperm densities detected by microscopic analysis and grey CASA were (9.23 +/- 8.01) x 10(6)/ml and (10.27 +/- 6.22) x 10(6)/ml, and the disparity rates of sperm density were 49.06% +/- 49.87% and 43.39% +/- 25.56% respectively.


CONCLUSIONS
Fluorescence CASA was more conformable to the requirements of latest WHO laboratory manual for the examination of human semen, and worth recommending for the clinical analysis of semen features, especially sperm density.
Jin
Wei
W
Department of Urology, Hospital of Peking Union Medical College, the Chinese Academy of Medical Sciences, Beijing 100730, China. jinwei_hy@sina.com
Chen
Song
S
Li
Hanzhong
H
D000328:Adult; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D006801:Humans; D008297:Male; D012661:Semen / Q000737:chemistry*; D013081:Sperm Motility
D004247:DNA