Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV.
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 degrees C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.
Darnell
Miriam E R
ME
Center for Biologics Evaluation and Research, US Food and Drug Administration, 8800 Rockville Pike, HFM448, Bethesda, MD 20892, USA.
Subbarao
Kanta
K
Feinstone
Stephen M
SM
Taylor
Deborah R
DR
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002522:Chlorocebus aethiops; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays; D014709:Vero Cells; D038301:Virus Inactivation
D000998:Antiviral Agents; D005557:Formaldehyde; D005976:Glutaral