Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae.
The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.
Bruisten
S M
SM
GG&GD Municipal Health Service, Public Health Laboratory, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. sbruisten@gggd.amsterdam.nl
Noordhoek
G T
GT
van den Brule
A J C
AJ
Duim
B
B
Boel
C H E
CH
El-Faouzi
K
K
du Maine
R
R
Mulder
S
S
Luijt
D
D
Schirm
J
J
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D001425:Bacterial Outer Membrane Proteins; C082716:cppB protein, Neisseria gonorrhoeae