title: Study on OELs for enzyme-containing detergent in China.
abstract: This study is aimed at setting occupational exposure levels for total detergent dust and enzymes in detergent industries. The study population consisted of 795 workers from four enzyme-containing detergent manufacturing plants (A1, A2, B1 and B2), and 156 control workers from an electronic assembly factory. Work environment monitoring was conducted using high volume of air sampler fro measuring the concentration of total dust (mg/m3), and analyzing the level of enzyme (ng/m3) by ELISA method. A standard questionnaires, pulmonary function test, and skin prick test are used to assess health effects. The levels of detergent total dust varied from 0.2 mg/m3 to 12.54 mg/m3. For enzyme levels, in A1, B1 and B2, the concentration ranged from non-detectable to 9.92 ng/m3 and in A2, the concentration was analyzed by enzyme activity methods and was expressed as Gu/m3 (1 Gu/m3 = 16 ng/m3). The concentration is between 0.16-31.36 ng/m3. Non-specific irritation rates in exposed workers were significantly higher than that in controls. Based on the data collected from A1, B1 and control plants, 95% benchmark dose lower bound were calculated as 1.17 mg/m3. The difference of pulmonary function between exposed workers and controls is not significant. The results of SPT showed that neither Savinase- nor Alcalase-induced sensitization was found in controls. The prevalence rates of sensitization for Savinase and Alcalase were ranged between 3.2% and 31% in all enzyme-containing detergent manufacturers investigated. No case of occupational asthma was observed. For total dust, 1 mg/m3 is suggested as permissible concentration-time weighted average (PC-TWA), and 2 mg/m3 as permissible concentration-short term exposure limit (PC-STEL). For the enzyme Subtilisins, 15 ng/m3 is suggested as PC-TWA, and 30 ng/m3 as PC-STEL.
authors.affiliation: School of Public Health Fudan University, Shanghai, PR China.
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
authors.forename: Y X
authors.initials: YX
authors.lastname: Liang
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: T Y
authors.initials: TY
authors.lastname: Jin
mesh_terms: D002681:China; D003902:Detergents / Q000009:adverse effects* / Q000592:standards; D004391:Dust; D004798:Enzymes / Q000009:adverse effects* / Q000592:standards; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D009784:Occupational Diseases / Q000139:chemically induced / Q000209:etiology; D016273:Occupational Exposure / Q000009:adverse effects* / Q000592:standards; D009787:Occupational Medicine / Q000592:standards; D012697:Serine Endopeptidases / Q000009:adverse effects / Q000592:standards
chemical_list: D003902:Detergents; D004391:Dust; D004798:Enzymes; D012697:Serine Endopeptidases; C032923:microbial serine proteinases