title: Inhibition of substrate-tumor cell adhesion under the effect of gnidilatimonoein purified from Daphne mucronata.
abstract: Alteration of the cell surface glycoproteins of cancerous cells correlate with malignancy potential. To evaluate the mediation of membrane glycoproteins in wehi-164 cancerous cells, under the effect of D. mucronata crude extract and one of its purified active components, gnidilatimonoein, their attachment to fibronectin-coated wells were investigated. The plant extract, 27 microl/ml (equivalent to 0.54 mg/of plant leaves powder per ml of culture medium), as well as gnidilatimonoein (0.94 microM), were capable of quenching by 58% and 64%, respectively, the attachment of wehi-164 cells to fibronectin-coated wells (4 microg/ml). In addition to alteration of cell adhesive properties, the morphology of the treated cells were significantly changed upon treatment with the non-toxic dose of the plant extract or gnidilatimonoein. While the untreated cells have polygonal shapes, the treated cells had spherical shapes.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran P.O. Box 13145-1384, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Mianabadi
authors.forename: R
authors.initials: R
authors.lastname: Yazdanparast
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor / Q000166:cytology* / Q000187:drug effects*; D032364:Daphne; D004224:Diterpenes / Q000494:pharmacology*; D005353:Fibronectins / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D004224:Diterpenes; D005353:Fibronectins; D010936:Plant Extracts; C482573:gnidilatimonoein