Inhibition of substrate-tumor cell adhesion under the effect of gnidilatimonoein purified from Daphne mucronata.
Alteration of the cell surface glycoproteins of cancerous cells correlate with malignancy potential. To evaluate the mediation of membrane glycoproteins in wehi-164 cancerous cells, under the effect of D. mucronata crude extract and one of its purified active components, gnidilatimonoein, their attachment to fibronectin-coated wells were investigated. The plant extract, 27 microl/ml (equivalent to 0.54 mg/of plant leaves powder per ml of culture medium), as well as gnidilatimonoein (0.94 microM), were capable of quenching by 58% and 64%, respectively, the attachment of wehi-164 cells to fibronectin-coated wells (4 microg/ml). In addition to alteration of cell adhesive properties, the morphology of the treated cells were significantly changed upon treatment with the non-toxic dose of the plant extract or gnidilatimonoein. While the untreated cells have polygonal shapes, the treated cells had spherical shapes.
Mianabadi
M
M
Institute of Biochemistry and Biophysics, University of Tehran P.O. Box 13145-1384, Tehran, Iran.
Yazdanparast
R
R
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor / Q000166:cytology* / Q000187:drug effects*; D032364:Daphne; D004224:Diterpenes / Q000494:pharmacology*; D005353:Fibronectins / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*
D004224:Diterpenes; D005353:Fibronectins; D010936:Plant Extracts; C482573:gnidilatimonoein