title: RecQ helicase enhances homologous recombination in plants.
abstract: RecQ helicase is a key component in the RecF pathway of Escherichia coli for initiation of homologous recombination. Here, we demonstrate that transient expression of RecQ gene in rice embryogenic cell increases the homologous recombination efficiency as much as 4-fold. Further experiments reveal that this effect is influenced by the RecQ dosage. Stable expression of RecQ in rice dramatically increases the homologous recombination events 20- to 40-fold in leaf tissue from different transgenic lines. This is the first evidence indicating that overexpression of RecQ gene can stimulate homologous recombination in plants.
authors.affiliation: Guangdong Key Lab of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China. hqli@scnu.edu.cn
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Li
authors.forename: Rie
authors.initials: R
authors.lastname: Terada
authors.forename: Mei-Ru
authors.initials: MR
authors.lastname: Li
authors.forename: Shigeru
authors.initials: S
authors.lastname: Iida
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism*; D010944:Plants / Q000235:genetics*; D053484:RecQ Helicases; D011995:Recombination, Genetic
chemical_list: D000251:Adenosine Triphosphatases; C506724:RecQ protein, E coli; D004265:DNA Helicases; D053484:RecQ Helicases