title: Analysis of the optimal blood sampling conditions for estimation of hematopoietic progenitor cell count by the SE-9000 automated hematolyzer.
abstract: We evaluated the optimal conditions for blood sampling for hematopoietic progenitor cells (HPCs) as estimated by the immature information program of the SE-9000 automated hematology analyzer. The HPC count was most stable when the blood samples were incubated at room temperature with ethylene-diaminetetraacetic acid dipotassium (EDTA-2K) as an anticoagulant. The HPC count should, however, be measured within 4 h after blood collection, even under optimal conditions. In contrast, the CD34+ cell count estimated by flow cytometric analysis was stable for at least 21 h after the blood samples were incubated with EDTA-2K at room temperature or 4 degrees C. When appropriate blood samples were used, the HPC count in the peripheral blood significantly correlated with the CD34+ cell count in the peripheral blood and in the apheresis yields (r = 0.798 and 0.635, respectively); therefore, the HPC count is a reliable predictor for initiation of apheresis procedures to obtain sufficient HPCs for peripheral blood stem cell transplantation.
authors.affiliation: Department of Transfusion Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nomura
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Kubota
authors.forename: Natsumi
authors.initials: N
authors.lastname: Baba
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Akira
authors.initials: A
authors.lastname: Kitanaka
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Taminato
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000925:Anticoagulants; D001781:Blood Component Removal; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D002648:Child; D002675:Child, Preschool; D005260:Female; D006405:Hematology / Q000295:instrumentation*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D017463:Receptors, Complement 3b / Q000032:analysis; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000925:Anticoagulants; D017463:Receptors, Complement 3b