Effects of protein kinase C on M-phase promoting factor in early development of fertilized mouse eggs.
The mechanism of development of mouse fertilized eggs from the one-cell stage to the two-cell stage remains unclear to date. In the present study, we have evaluated protein kinase C (PKC) and M-phase promoting factor (MPF) kinase activity in fertilized mouse eggs treated with a PKC modulator. PKC and MPF activity have similar activity. The two subunits of MPF, p34(cdc2) and cyclin B, were shown to be included in the substrates phosphorylated by PKC in fertilized mouse eggs, while PKC modulator affected the electrophoretic mobility shift of cdc2 and cdc25C by dephosphorylation and phosphorylation. These results clearly indicate that PKC may affect the progression of the cell cycle through post-translational modification of MPF activity.
Yu
Bing-zhi
BZ
Department of Biochemistry, China Medical University, Shenyang, 110001, People's Republic of China. ybzbiochem@yeah.net
Zheng
Jie
J
Yu
Ai-ming
AM
Shi
Xiu-yan
XY
Liu
Ying
Y
Wu
Di-di
DD
Fu
Wei
W
Yang
Jianing
J
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D016203:CDC2 Protein Kinase / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D004075:Diglycerides / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005307:Fertilization in Vitro; D006657:Histones / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D019311:Staurosporine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015053:Zygote / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D020687:cdc25 Phosphatases / Q000378:metabolism
D000546:Amanitins; D018797:Cell Cycle Proteins; D004075:Diglycerides; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C000715528:Msln protein, mouse; D047428:Protein Kinase Inhibitors; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor; C517010:Cdc25c protein, mouse; D020687:cdc25 Phosphatases; D019311:Staurosporine; D000090204:Mesothelin; D013755:Tetradecanoylphorbol Acetate