title: Transcription by Methanothermobacter thermautotrophicus RNA polymerase in vitro releases archaeal transcription factor B but not TATA-box binding protein from the template DNA.
abstract: Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The results reported establish the fate of Methanothermobacter thermautotrophicus TBP and TFB following transcription initiation by M. thermautotrophicus RNAP in vitro. TFB is released after initiation, during extension of the transcript from 4 to 24 nucleotides, but TBP remains bound to the template DNA. Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation.
authors.affiliation: Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Xie
authors.forename: John N
authors.initials: JN
authors.lastname: Reeve
mesh_terms: D019843:Archaeal Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D017013:Methanobacteriaceae / Q000201:enzymology* / Q000235:genetics*; D035181:TATA-Box Binding Protein / Q000378:metabolism*; D035581:Transcription Factor TFIIB / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D019843:Archaeal Proteins; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; C106466:transcription factor B, Archaea; D004247:DNA; D012321:DNA-Directed RNA Polymerases