The effects of the caffeic acid phenethyl ester (CAPE) on erythrocyte membrane damage after hind limb ischaemia-reperfusion.
Reactive oxygen species have been implicated in pathogenesis injury after ischaemia-reperfusion (I/R). Caffeic Acid Phenethyl Ester (CAPE), an active component of honeybee propolis extract, exhibits antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effects of CAPE on erythrocyte membrane damage after hind limb I/R. Rats were divided into two groups: I/R and I/R with CAPE pre-treatment. They were anaesthetized with intramuscular ketamine 100 mg kg(-1). A 4-h I/R period was performed on the right hind limb of all animals. In the CAPE-treated group, animals received CAPE 10 microm by intraperitoneal injection 1 h before the reperfusion. At the end of the reperfusion period, a midsternotomy was performed. A 5-ml blood sample was withdrawn from the ascending aorta for biochemical assays. Serum and erythrocyte membrane MDA levels were significantly lower in the CAPE-treated group when compared to the I/R group ( p = 0.001 and p<0.001, respectively). Erythrocyte membrane Na(+)-K(+) ATPases activity in the CAPE-treated group was significantly higher than the I/R group ( p<0.001). In conclusion, CAPE seems to be effective in protecting against oxidative stress. Therefore, we suggest that in order to decrease I/R injury, pre-administration of CAPE may be a promising agent for a variety of conditions associated with I/R.
Tamer
Lülüfer
L
Department of Biochemistry, Medical Faculty, Mersin University, 33079 Mersin, Turkey. lutamer@yahoo.com
Sucu
Nehir
N
Ercan
Bahadir
B
Unlü
Ali
A
Calikoğlu
Mukadder
M
Bilgin
Ramazan
R
Değirmenci
Ulaş
U
Atik
Uğur
U
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D006614:Hindlimb / Q000098:blood supply; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000975:Antioxidants; D002109:Caffeic Acids; D008315:Malondialdehyde; D000254:Sodium-Potassium-Exchanging ATPase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol