title: [The relationship between p120ctn translocation and malignant features of hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the effect of catenin p120 (p120ctn) translocation on the malignant features of hepatocellular carcinoma and its interrelation with beta-catenin in E-cadherin-mediated cell signaling.
  
  
  METHODS
  Expression and translocation of p120ctn, tyrosine phosphorylation, and its binding capacity to E-cadherin were detected by DNA transfection, immunoblotting and immunoprecipitation. Cellular localization of p120ctn and beta-catenin was detected by immunofluorescent microscopy. Cell adhesion, cell migration and cell proliferation were also studied.
  
  
  RESULTS
  Expression of p120ctn increased after cells transfected with p120ctn isoform 3A, and it was located mainly at cell-cell contact region. Its binding to E-cadherin was enhanced. After EGF stimulation, tyrosine phosphorylation of p120ctn was increased, membrane expression of p120ctn and beta-catenin was decreased while cytosol expression was increased. It was translocated into the nucleus, cell adhesiveness was increased but mobility decreased. With over-expression of p120ctn, beta-catenin was recruited by nucleus export. Cell proliferation was reduced but it was increased after EGF treatment.
  
  
  CONCLUSIONS
  p120tn plays an important role in cell adhesion, migration and proliferation of hepatocellular carcinoma, and its tyrosine phosphorylation might contribute to this mechanism. There might be a competitive relationship between p120ctn and beta-catenin.
authors.affiliation: Department of Central Laboratory, Guangxi Nationalities Hospital, Nanning 530001, China. huayih@yahoo.com
authors.forename: Hua-yi
authors.initials: HY
authors.lastname: Huang
authors.forename: Chao-zan
authors.initials: CZ
authors.lastname: Nong
authors.forename: Wei-sheng
authors.initials: WS
authors.lastname: He
authors.forename: Ling-xiao
authors.initials: LX
authors.lastname: Guo
authors.forename: Shao-yun
authors.initials: SY
authors.lastname: Nong
authors.forename: Li-li
authors.initials: LL
authors.lastname: Pan
authors.forename: Xi-liang
authors.initials: XL
authors.lastname: Zha
mesh_terms: D015820:Cadherins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D051177:Catenins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D021381:Protein Transport; D015534:Trans-Activators / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D051176:beta Catenin; D000095002:Delta Catenin
chemical_list: C495270:CTNNB1 protein, human; D015820:Cadherins; D051177:Catenins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D015534:Trans-Activators; D051176:beta Catenin; D014443:Tyrosine; D004815:Epidermal Growth Factor; D000095002:Delta Catenin; C000722347:CTNND1 protein, human