title: Using analytical multidimensional isocratic HPLC methods of separation to isolate active constituents in natural products.
abstract: Multidimensional reversed-phase HPLC was employed to isolate the active constituents from a crude extract of Clerodendrum floribundum R. Br. These active constituents were known to have bioactivity against the enzyme xanthine oxidase and potentially could be employed in the treatment of gout. Using a multidimensional separation approach, rapid isolation of the active constituents was achieved from a complex sample matrix. As each separation dimension was isocratic, no equilibration time was required and consequently the technique shows promise in the scale up to preparative levels where high throughput is important.
authors.affiliation: School of Science, Food and Horticulture, University of Western Sydney, Hawkesbury Campus, Locked Bag 1797, Penrith South, NSW 1797, Australia.
authors.forename: Victor
authors.initials: V
authors.lastname: Wong
authors.forename: Alan P
authors.initials: AP
authors.lastname: Sweeney
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Shalliker
mesh_terms: D001688:Biological Products / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D032409:Clerodendrum / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D006073:Gout / Q000188:drug therapy; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D001688:Biological Products; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014969:Xanthine Oxidase