Adverse reactions to drugs: in vitro studies with isolated cells.
OBJECTIVE
Drug-induced adverse reactions can be allergic or pseudoallergic in nature, in this study the histamine releasing ability of 4 radiographic contrast media and 2 opioid analgesics was tested on a variety of mast cell containing cell suspensions.


METHODS
Mast cell containing cell suspensions were obtained from porcine lung, liver, kidneys and heart, as well as rat lung and rat peritoneal lavage.


METHODS
Cells were incubated for 10 min with Angiographin (amidotrizoate), Hexabrix (ioxaglate), Rayvist (ioglycate) or Telebrix (ioxithalamate) all 10-500 microl/ml or with levomethadone (0.1-5 mM) or pethidine (0-10 mM).


METHODS
Histamine was measured using an automated fluorometric method and percentage histamine release calculated.


RESULTS
All agents caused histamine release from porcine cells and rat lung cells. However, rat peritoneal mast cells were refractory to the action of pethidine. Cardiac cells were the most sensitive to the radiographic contrast media but the least sensitive to the opioid analgesics.


CONCLUSIONS
The data indicate that mast cells isolated from animal models can provide an indication of those agents likely to induce an adverse pseudoallergic reaction. However, these data should be used together with those obtained from human mast cells, in vivo animal experiments as well as studies using human volunteers and clinical trials as advocated in the Marburg Model.
Ennis
M
M
Respiratory Research Group, Department of Clinical Biochemistry and Metabolic Medicine, Institute of Clinical Science, The Queen's University of Belfast, Grosvenor Road, BT12 6BJ, Belfast, Northern Ireland, UK. m.ennis@qub.ac.uk
Nehring
E
E
Schneider
C
C
D000701:Analgesics, Opioid / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D018893:Bronchoalveolar Lavage; D002460:Cell Line; D003287:Contrast Media / Q000009:adverse effects* / Q000494:pharmacology; D005260:Female; D006636:Histamine Release / Q000187:drug effects / Q000276:immunology; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology*; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008168:Lung / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D010533:Peritoneal Lavage; D011859:Radiography / Q000295:instrumentation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
D000701:Analgesics, Opioid; D003287:Contrast Media