title: [Anti-SARS virus activities of different recombinant human interferons in cell culture system].
abstract: BACKGROUND
  To study the anti-SARS virus activities of different recombinant human interferons on the cell culture system.
  
  
  METHODS
  Anti-SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda) cell culture.
  
  
  RESULTS
  The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5+/-129.5) IU/ml, (149.0+/-71.7) IU/ml, (69.5+/-61.5) IU/ml, (87.3+/-47.1) IU/ml, respectively or (0.6+/-0.5) ng/ml, (10.6+/-5.1) ng/ml, (3.5+/-3.1) ng/ml, (0.9+/-0.5) ng/ml, respectively.
  
  
  CONCLUSIONS
  All the tested recombinant interferons showed anti-SARS virus activities on the Rda cell culture with different sensitivities.
authors.affiliation: Beijing Jin Di Ke Biotechnology Institute, State Research Center for Biotechnological Engineering, Beijing 100176, China.
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
authors.forename: Li-lan
authors.initials: LL
authors.lastname: Zhang
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Zhi-ai
authors.initials: ZA
authors.lastname: Yu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Liu
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Wu-ping
authors.initials: WP
authors.lastname: Li
authors.forename: Cheng-hai
authors.initials: CH
authors.lastname: Zhang
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Yue-long
authors.initials: YL
authors.lastname: Shu
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Duan
authors.forename: De-xin
authors.initials: DX
authors.lastname: Li
authors.forename: Yun-de
authors.initials: YD
authors.lastname: Hou
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D011994:Recombinant Proteins; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects*; D045169:Severe Acute Respiratory Syndrome / Q000188:drug therapy / Q000821:virology
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins