Cloning, characterization, and functional expression of Taenia solium calreticulin.
Calreticulin is an endoplasmic reticulum protein involved in the homeostasis of intracellular Ca++ and other physiological processes. A complementary DNA clone containing the complete coding sequence of Taenia solium calreticulin (TsCRT) was isolated and characterized. Recombinant TsCRT was expressed in bacteria as a 50-kDa protein that specifically bound calcium when tested in a radioassay. The deduced amino acid sequence has 47-50% identity with other reported calreticulins. Poor recognition of TsCRT by human and pig sera with confirmed cysticercosis discourages its use for diagnosis of the disease. However, further characterization and localization studies could provide insights into the role of TsCRT in T. solium physiology and host-parasite interactions.
Mendlovic
Fela
F
Departamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF 04510, México.
Ostoa-Saloma
Pedro
P
Solís
Carlos F
CF
Martínez-Ocaña
Joel
J
Flisser
Ana
A
Laclette
Juan P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D015870:Gene Expression; D006790:Host-Parasite Interactions; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D015183:Restriction Mapping; D016415:Sequence Alignment; D013552:Swine; D041201:Taenia solium / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D037282:Calreticulin; D018076:DNA, Complementary; D018509:DNA, Helminth; D007106:Immune Sera