[The influence of temperature on the protein expression of human lung cancer cell line A549].
The study on proteome of human cancer is helpful to explain its pathogenesis and make good effect on its prevention and cure. We compared the 2-DE maps of whole proteins of human lung cancer line A549 at 37 degrees C, 42 degrees C and 45 degrees C for the purpose of studying the expression of its heat shock proteins. Three temperature-sensitive differential spots were obtained and named as P1, P2, P3, respectively. Analyzed by MALDI-TOF-MS and Peptident software searched in the SWISS-PROT database, the three differential proteins were elementarily identified. P1 matches with two proteins belong to the Aldo-keto reductase family, P2 may be a new protein and P3 is Zinc finger protein 11A.
Chen
Zi Jun
ZJ
The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005.
Wang
San Ying
SY
Chen
Jing An
JA
Peng
Xuan Xian
XX
D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins