title: Determination of SARS-coronavirus by a microfluidic chip system.
abstract: We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-CoV). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Xiaomian
authors.initials: X
authors.lastname: Zhou
authors.forename: Dayu
authors.initials: D
authors.lastname: Liu
authors.forename: Runtao
authors.initials: R
authors.lastname: Zhong
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Dai
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Xia
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaodai
authors.initials: X
authors.lastname: Mei
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D001344:Autopsy; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008168:Lung / Q000821:virology; D046208:Microchip Analytical Procedures / Q000379:methods*; D016133:Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*