Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Studies of SARS coronavirus (SARS-CoV)-the causative agent of severe acute respiratory syndrome (SARS)-have been hampered by its high transmission rate and the pathogenicity of this virus. To permit analysis of the host range and entry mechanism of SARS-CoV, we incorporated the humanized SARS-CoV spike (S) glycoprotein into HIV particles to generate a highly infectious SARS-CoV pseudotyped virus. The infection on Vero E6-a permissive cell line to SARS-CoV-could be neutralized by sera from convalescent SARS patients, and the entry was a pH-dependent process. With these highly infectious SARS-CoV pseudotypes, several cell lines derived from various tissues were revealed as susceptible to SARS-CoV, which were highly corresponding to the expression pattern of virus's receptor angiotensin-converting enzyme 2 (ACE2). In addition, we also demonstrated angiotensin 1 converting enzyme (ACE)-the homologue of ACE2 could not function as a receptor for SARS-CoV.
Nie
Yuchun
Y
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Wang
Peigang
P
Shi
Xuanling
X
Wang
Guangwen
G
Chen
Jian
J
Zheng
Aihua
A
Wang
Wei
W
Wang
Zai
Z
Qu
Xiuxia
X
Luo
Min
M
Tan
Lei
L
Song
Xijun
X
Yin
Xiaolei
X
Chen
Jianguo
J
Ding
Mingxiao
M
Deng
Hongkui
H
D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002678:Chimera / Q000235:genetics; D002522:Chlorocebus aethiops; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D006678:HIV / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D007703:Peptidyl-Dipeptidase A; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics / Q000502:physiology; D019065:Virus Assembly
D004274:DNA, Recombinant; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; D002268:Carboxypeptidases; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; C000705328:Ace2 protein, mouse; D000085962:Angiotensin-Converting Enzyme 2