[Effect of Xiangdan Injection on mRNA expression of endothelial vaso-active factors of patients with coronary heart disease and blood stasis].
OBJECTIVE
To evaluate the effect of Xiangdan Injection on mRNA expression of the endothelial vaso-active factors of patients with coronary heart disease and blood stasis.


METHODS
Fifty-six patients were randomly divided into two groups:twenty-eight patients were treated according to the therapeutic guide for coronary heart disease as the control group and 28 were given the same treatment plus Xiangdan Injection as the treated group. The expressions of ET-1 and eNOS mRNA were examined with RT-PCR before experiment and ten days later.


RESULTS
The positive rate of eNOS mRNA of the treated group increased, while the positive rate of ET-1 mRNA of the treated group decreased after ten day's treatment, with significant differences as compared with that before the experiment. Xiangdan Injection up-regulated the eNOS mRNA expression and suppressed the ET-1 mRNA expression. Changes of expression were not observed in the control group.


CONCLUSIONS
Xiangdan Injection improves the endothelial function of patients with coronary heart disease and blood stasis by regulating the expressions of ET-1 and eNOS mRNA.
Wu
Shi-Da
SD
Department of Cardiology, Chengdu First People's Hospital, Chengdu, Sichuan Province 610016, China.
Wang
Jing
J
Chen
Shou-Chun
SC
Xu
Jun-Bo
JB
Zheng
Qiang
Q
Yan
Ya-Fei
YF
Wen
Tian-Ming
TM
Tang
Yan-Rong
YR
D000368:Aged; D000369:Aged, 80 and over; D003327:Coronary Disease / Q000188:drug therapy* / Q000378:metabolism; D003328:Coronary Thrombosis / Q000188:drug therapy / Q000378:metabolism; D019332:Endothelin-1 / Q000235:genetics*; D005260:Female; D006801:Humans; D007267:Injections; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D012333:RNA, Messenger / Q000032:analysis*
D019332:Endothelin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III