[Relationship among expression of the VEGF gene and MVD with cervical lymph nodes metastasis in laryngeal squamous cell carcinoma].
OBJECTIVE
To study the relation among the expression of the vascular endothelial growth factor (VEGF) gene and microvessel Density (MVD) with cervical lymph nodes metastasis in laryngeal squamous cell carcinoma (LSCC).


METHODS
Appling semi-RT-PCR technique and immunohistochemistry detected the expression of VEGFmRNA and protein and MVD count in 60 cases with LSCC.


RESULTS
The expression of VEGF and MVD was both higher either at molecular level or at the protein level in the group with cervical lymph nodes metastasis than the those with cervical lymph nodes-negative metastasis. The overexpression of VEGF had a positive correlation with the higher MVD count and were both related to cervical lymph nodes metastasis in the 60 cases with LSCC.


CONCLUSIONS
There was a significant correlation between the expression of VEGF and MVD, which suggested that the overexpression of VEGF was essential to microangiogenesis of LSCC, and the both were related to cervical lymph nodes metastasis and would become a biologic indexes to prognosis of LSCC. Antiangiogenic therapy with specific antibodies to VEGF leads to inhibit angiogenesis and prevent tumor from growth and metastasis, which might have a bright future in clinical application.
Wang
Zhanping
Z
Department of Otolaryngology, Benxi Central Hospital, Benxi, 117000, China.
Ji
Wenyue
W
Tang
Qiaofei
Q
Pan
Zimin
Z
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism* / Q000556:secondary; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009333:Neck; D009389:Neovascularization, Pathologic; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics
D042442:Vascular Endothelial Growth Factors