Identification and characterization of the rlp1+, the novel Rad51 paralog in the fission yeast Schizosaccharomyces pombe.
A new DNA repair gene from fission yeast Schizosaccharomyces pombe rlp1+ (RecA-like protein) has been identified. Rlp1 shows homology to RecA-like proteins, and is the third S. pombe Rad51 paralog besides Rhp55 and Rhp57. The new gene encodes a 363 aa protein with predicted Mr of 41,700 and has NTP-binding motif. The rlp1Delta mutant is sensitive to methyl methanesulfonate (MMS), ionizing radiation (IR), and camptothecin (CPT), although to a lesser extent than the deletion mutants of rhp55+ and rhp51+ genes. In contrast to other recombinational repair mutants, the rlp1Delta mutant does not exhibit sensitivity to UV light and mitomycin C (MMC). Mitotic recombination is moderately reduced in rlp1 mutant. Epistatic analysis of MMS and IR-sensitivity of rlp1Delta mutant indicates that rlp1+ acts in the recombinational pathway of double-strand break (DSB) repair together with rhp51+, rhp55+, and rad22+ genes. Yeast two-hybrid analysis suggests that Rlp1 may interact with Rhp57 protein. We propose that Rlp1 have an accessory role in repair of a subset of DNA damage induced by MMS and IR, and is required for the full extent of DNA recombination and cell survival under condition of a replication fork collapse.
Khasanov
Fuat K
FK
Institute of Gene Biology, Russian Academy of Sciences, Molecular Biology of DNA Repair, Vavilov Street 34/5, 119334 Moscow, Russia.
Salakhova
Albina F
AF
Chepurnaja
Olga V
OV
Korolev
Vladimir G
VG
Bashkirov
Vladimir I
VI
D000251:Adenosine Triphosphatases / Q000502:physiology; D000595:Amino Acid Sequence; D002166:Camptothecin / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair; D004271:DNA, Fungal / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology; D006801:Humans; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D011839:Radiation, Ionizing; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000187:drug effects; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; C113187:XRCC2 protein, human; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases; C499692:Rlp1 protein, S pombe; D000251:Adenosine Triphosphatases; C442472:Rhp57 protein, S pombe; D002166:Camptothecin