title: Phenotypic and genotypic characterization of beta-D-glucuronidase-positive Shiga toxin-producing Escherichia coli O157:H7 isolates from deer.
abstract: Beta-glucuronidase-positive (GUD+) Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from both an asymptomatic woman and uncooked deer meat in her possession in Hokkaido, Japan. The phenotypic and genotypic characteristics of the two isolates were identical or closely related, indicating probable transmission of the deer isolate to the woman. Moreover, several other GUD+ STEC O157:H7 strains investigated belonged to the distinct atypical GUD+ STEC O157:H7 group that has been identified previously. This is the first report that deer can be a reservoir of GUD+ STEC O157:H7 in Japan.
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nagano
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirochi
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wakamori
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Takeshi
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
authors.forename: Shoki
authors.initials: S
authors.lastname: Yano
mesh_terms: D000818:Animals; D003670:Deer / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D005966:Glucuronidase / Q000032:analysis*; D006801:Humans; D010641:Phenotype; D022621:Shiga Toxin / Q000096:biosynthesis*
chemical_list: D022621:Shiga Toxin; D005966:Glucuronidase