Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.
Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.
De Oliveira
Valeria Cora
VC
Département de Biologie Moléculaire Végétale, Université de Lausanne, CH-1015 Lausanne, Switzerland.
Maeda
Isamu
I
Delessert
Syndie
S
Poirier
Yves
Y
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000333:Aeromonas / Q000201:enzymology / Q000235:genetics; D002244:Carbon / Q000378:metabolism*; D003001:Cloning, Molecular; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D004271:DNA, Fungal; D011994:Recombinant Proteins; D002244:Carbon; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase