title: Effects of low sulfur fuel and a catalyzed particle trap on the composition and toxicity of diesel emissions.
abstract: In this study we compared a "baseline" condition of uncontrolled diesel engine exhaust (DEE) emissions generated with current (circa 2003) certification fuel to an emissions-reduction (ER) case with low sulfur fuel and a catalyzed particle trap. Lung toxicity assessments (resistance to respiratory viral infection, lung inflammation, and oxidative stress) were performed on mice (C57Bl/6) exposed by inhalation (6 hr/day for 7 days). The engine was operated identically (same engine load) in both cases, and the inhalation exposures were conducted at the same exhaust dilution rate. For baseline DEE, this dilution resulted in a particle mass (PM) concentration of approximately 200 microg/m3 PM, whereas the ER reduced the PM and almost every other measured constituent [except nitrogen oxides (NOx)] to near background levels in the exposure atmospheres. These measurements included PM, PM size distribution, PM composition (carbon, ions, elements), NOx, carbon monoxide, speciated/total volatile hydrocarbons, and several classes of semivolatile organic compounds. After exposure concluded, one group of mice was immediately sacrificed and assessed for inflammation and oxidative stress in lung homogenate. Another group of mice were intratracheally instilled with respiratory syncytial virus (RSV), and RSV lung clearance and inflammation was assessed 4 days later. Baseline DEE produced statistically significant biological effects for all measured parameters. The use of low sulfur fuel and a catalyzed trap either completely or nearly eliminated the effects.
authors.affiliation: Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA. jmcdonal@lrri.org
authors.forename: Jacob D
authors.initials: JD
authors.lastname: McDonald
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Harrod
authors.forename: JeanClare
authors.initials: J
authors.lastname: Seagrave
authors.forename: Steven K
authors.initials: SK
authors.lastname: Seilkop
authors.forename: Joe L
authors.initials: JL
authors.lastname: Mauderly
mesh_terms: D000818:Animals; D002384:Catalysis; D019570:Inhalation Exposure; D008168:Lung / Q000187:drug effects / Q000473:pathology; D019216:Metals, Heavy / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D010316:Particle Size; D012016:Reference Values; D013455:Sulfur / Q000032:analysis*; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D001335:Vehicle Emissions; D013455:Sulfur