title: Mesengenic progenitor cells derived from human placenta.
abstract: Progenitor cells with differentiation capacity along multiple mesengenic lineages are attractive tools for numerous purposes in regenerative medicine. Such mesengenic progenitor cells have been isolated from adult mammalian bone marrow, and we here report placental tissue as an alternative source for these cells. By means of dissection/proteinase digestion techniques, high numbers of viable mononuclear cells were harvested from human placenta at term, and a mesenchymal cell population with characteristic expression of CD9, CD29, and CD73 was obtained in culture. The in vitro growth behavior of such placenta-derived mesengenic cells was similar to that of human bone marrow mesengenic progenitor cells. After in vitro propagation for more than three passages the cells were exclusively of maternal origin. Differentiation experiments showed differentiation potential along osteogenic, chondrogenic, adipogenic, and myogenic lineages. In conclusion, we propose human term placenta as an easily accessible, ample source of multipotent mesengenic progenitor cells.
authors.affiliation: Department of Hematology and Oncology, Georg August University Goettingen, 37075 Goettingen, Germany. gwulf@med.uni-goettingen.de
authors.forename: Gerald G
authors.initials: GG
authors.lastname: Wulf
authors.forename: Volker
authors.initials: V
authors.lastname: Viereck
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hemmerlein
authors.forename: Detlef
authors.initials: D
authors.lastname: Haase
authors.forename: Katalin
authors.initials: K
authors.lastname: Vehmeyer
authors.forename: Tobias
authors.initials: T
authors.lastname: Pukrop
authors.forename: Bertram
authors.initials: B
authors.lastname: Glass
authors.forename: Günter
authors.initials: G
authors.lastname: Emons
authors.forename: Lorenz
authors.initials: L
authors.lastname: Trümper
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000943:Antigens, Differentiation / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D010920:Placenta / Q000166:cytology* / Q000502:physiology*; D011247:Pregnancy; D013048:Specimen Handling / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000943:Antigens, Differentiation