title: [Establishment of primary cultured neurons model of mouse embryo with HSV-1 infection].
abstract: BACKGROUND
  To establish a neurons model for herpes simplex virus type 1 (HSV-1) infection using cultures of mouse embryo.
  
  
  METHODS
  The cortical neuron of 14-16 day old mouse embryo were cultured and infected with HSV-1. Microscopic examination, immunofluorescent test, MTT colorimetric analysis and flowcytometric assay were used to detect the immunofluorescent reaction of normal and infected neurons and astrocytes, respectively. The percentage of the two kinds of cells were measured.
  
  
  RESULTS
  The mouse neurons could proliferate well in vitro. The neurons amount met the requirement of cytological experiment after treatment with Ara-C in the tissue culture. HSV-1 could infect the neurons directly, the activity of the infected neurons was obviously reduced.
  
  
  CONCLUSIONS
  HSV-1 could successfully infect the primary cultured neurons. The model may provide a useful approach for studying the pathogenesis of herpes simplex virus encephalitis.
authors.affiliation: Department of Neurology, Beijing Friendship Hospital, Beijing 100050, China.
authors.forename: Wei-rong
authors.initials: WR
authors.lastname: Li
authors.forename: Jia-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: De-xin
authors.initials: DX
authors.lastname: Wang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000821:virology; D005260:Female; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D009474:Neurons / Q000821:virology*