[Larval cells of sponge Halisarca dujardini (Demospongiae, Halisarcida). II. Some cell types marked by polyclonal antibodies].
The recent morphological and experimental data concerning the involvement of flagellated cells in sponge larvae are contradictory and testify to or against the germinal layers inversion. A study of morphogenetic processes in sponges, in particular larval metamorphosis, is complicated by difficulties in identification and succession of certain cell types. It is possible to trace the destiny of flagellated and other larval cells by marking them with antibodies (AB) specified for each cell type. We separated larval and adult sponge cells of Halisarca dujardini in percoll density gradient and obtained polyclonal AB for the majority of these cell types. The protein pattern of larval flagellated cells differed significantly from that of other cell types. The major proteins of flagellated, collencyte-like and spherulous cells were used to raise the corresponding AB. Immunoblot showed all AB to be specific for certain proteins and suitable for immunofluorescence. The AB for flagellated cells reacted with the apical cytoplasm, but not with the flagellum, the AB for major protein of collencyte-like cells stained cytoplasm granules. The AB for spherulous cells of the adult sponge reacted with larval spherulous cells supposed to be of maternal origin. So, the method of cell marking with specific polyclonal AB can facilitate analysis of the layers inversion problem, as well as elucidate the degree of cell differentiation in larvae, their conformity to cells of the adult sponge or their provisional destiny.
Mukhina
Iu I
IuI
Podgornaia
O I
OI
Efremova
S M
SM
D000818:Animals; D000906:Antibodies; D003593:Cytoplasm / Q000737:chemistry; D005407:Flagella; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D015151:Immunoblotting; D007814:Larva / Q000737:chemistry / Q000166:cytology; D011161:Porifera / Q000737:chemistry / Q000166:cytology*; D011506:Proteins / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D011506:Proteins