title: PCR-based identification of hyperthermophilic archaea of the family Thermococcaceae.
abstract: A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5'-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3') and TcPc 589R (5'-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3') was developed and used for identification of new isolates.
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, prospekt 60-letiya Oktyabrya 7/2, Moscow, 117312 Russia.
authors.forename: Galina B
authors.initials: GB
authors.lastname: Slobodkina
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Chernyh
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Slobodkin
authors.forename: Irina V
authors.initials: IV
authors.lastname: Subbotina
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Lebedinsky
mesh_terms: D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D005843:Geography; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D019710:Thermococcaceae / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers