Endothelial NO synthase genotype and risk of preeclampsia: a multicenter case-control study.
Polymorphisms in the endothelial NO synthase (eNOS) gene have been evaluated as risk factors for preeclampsia. However, data from small studies are conflicting. We assessed whether eNOS genotypes alter the risk of preeclampsia in a population in which the incidence of this disorder is high. A total of 844 young pregnant women (322 preeclamptic and 522 controls) were recruited from 5 cities. Genotyping for the Glu298Asp, intron-4 and -786T-->C polymorphisms in the eNOS gene was conducted. Multivariate odds ratios (ORs) were obtained to estimate the association of individual polymorphisms and haplotypes with preeclampsia risk. No increase in the risk of preeclampsia for the intron-4 or -786T-->C polymorphisms was observed under any model of inheritance. In contrast, in women homozygous for the Asp298 allele, the adjusted OR for preeclampsia was 4.60 (95% confidence interval [CI], 1.73 to 12.22) compared with carriers of the Glu298 allele. After a multivariate analysis, carriage of the "Asp298-786C-4b" haplotype was also associated with increased risk of preeclampsia (OR, 2.11 [95% CI, 1.33 to 3.34]) compared with carriers of the "Glu298-786T-4b" haplotype. The eNOS Glu298Asp polymorphism and the Asp298-786C-4b haplotype are risk factors for preeclampsia.
Serrano
Norma C
NC
Genetics and Human Biology Laboratory, Department of Medicine at Universidad Autónoma de Bucaramanga, Colombia, Campus el Bosque, Calle 157 No. 19-55 Cañaveral Parque, Colombia. nserrano@unab.edu.co
Casas
Juan P
JP
Díaz
Luis A
LA
Páez
Carolina
C
Mesa
Clara M
CM
Cifuentes
Rodrigo
R
Monterrosa
Alvaro
A
Bautista
Alejandro
A
Hawe
Emma
E
Hingorani
Aroon D
AD
Vallance
Patrick
P
López-Jaramillo
Patricio
P
D000328:Adult; D016022:Case-Control Studies; D003105:Colombia / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D011247:Pregnancy; D012307:Risk Factors
C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III