DOMINO1, a member of a small plant-specific gene family, encodes a protein essential for nuclear and nucleolar functions.
Arabidopsis embryos carrying the domino1 mutation grow slowly in comparison with wild type embryos and as a consequence reach only the globular stage at desiccation. The primary defect of the mutation at the cellular level is the large size of the nucleolus that can be observed soon after fertilization in the nuclei of both the embryo and the endosperm. The ultrastructure of mutant nucleoli is drastically different from wild type and points to a fault in ribosome biogenesis. DOMINO1 encodes a protein, which belongs to a plant-specific gene family sharing a common motif of unknown function, present in the tomato DEFECTIVE CHLOROPLASTS AND LEAVES (LeDCL) protein. Using a GFP protein fusion, we show that DOMINO1 is targeted to the nucleus. We propose that inactivation of DOMINO1 has a negative effect on ribosome biogenesis and on the rate of cell division.
Lahmy
Sylvie
S
Laboratoire Génome et Développement des Plantes, CNRS-UMR 5096, Université de Perpignan, 52 Avenue de Villeneuve, 66860 Perpignan-cedex, France.
Guilleminot
Jocelyne
J
Cheng
Ching-Ming
CM
Bechtold
Nicole
N
Albert
Sylvie
S
Pelletier
Georges
G
Delseny
Michel
M
Devic
Martine
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D002466:Cell Nucleolus / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D017124:Conserved Sequence; D005306:Fertilization; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins