Amperometric glucose biosensor based on assisted ion transfer through gel-supported microinterfaces.
A novel amperometric glucose sensor was developed based on the facilitated proton transfer across microinterfaces between two immiscible electrolyte solutions. The combination of a 1,3:2,4-dibenzylidene sorbitol/2-nitrophenyl octyl ether gel membrane and 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine as the ionophore allows the transfer of protons from water to the gellified organic phase; the gel membrane is supported on arrays of microholes drilled on a polyester film. The protons are generated as the result of the dissociation of gluconic acid produced during the enzymatic degradation of glucose by glucose oxidase. The characteristics of the glucose sensor were investigated using several experimental conditions, namely, the concentration of ligand and enzyme. The electrochemical response is typical of an enzymatic electrode and displays a linear behavior in the range 0.2-3 mM glucose. The effect of the experimental parameters of the voltammetric technique was also optimized with the aim of improving sensor sensitivity.
Pereira
Carlos M
CM
Departamento de Química, CIQ-L4, Faculdade de Ciências da Universidade do Porto, R. do Campo Alegre, 687, 4169-007 Porto, Portugal.
Oliveira
Joaquim M
JM
Silva
Ricardo M
RM
Silva
Fernando
F
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D005949:Glucose Oxidase; D005947:Glucose