title: [Effect of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues].
abstract: OBJECTIVE
  To investigate the effects of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues.
  
  
  METHODS
  Observations were made on the relaxing effect of chuanxiongzine on phenylephrine (PE)-precontracted corpus cavernosum and cavernosal strips preincubated with L-NAME, and ODQ, using endothelium removal and experimental method of smooth muscle strips. The effect of chuanxiongzine on cAMP and cGMP levels in corpus cavernosum was measured by 125I radioimmunoassay.
  
  
  RESULTS
  Chuanxiongzine had concentration-dependent relaxing effect on the cavernosal muscle contraction induced by PE (EC50 1.58 x 10(-4) mol/L). This relaxing effect was partially antagonized by ODQ and not blocked by L-NAME or endothelium removal. In PE-precontracted cavernosal strips, chuanxiongzine induced relaxation accompanied with an increase in cAMP and cGMP levels.
  
  
  CONCLUSIONS
  Chuanxiongzine was effective in relaxing cavernosal muscle precontracted by PE in vitro. And its relaxing effect may be enhanced by increasing cGMP and cAMP levels in the corpus cavernosum.
authors.affiliation: School of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Xiao
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.forename: Chunping
authors.initials: C
authors.lastname: Yin
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Longchang
authors.initials: L
authors.lastname: Fan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhangqun
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D010413:Penis / Q000187:drug effects* / Q000502:physiology; D011719:Pyrazines / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D011719:Pyrazines; D000242:Cyclic AMP; D006152:Cyclic GMP; C017953:tetramethylpyrazine