Chemical methods for peptide-oligonucleotide conjugate synthesis.
Methods of peptide-oligonucleotide conjugate synthesis are presented that may be useful in the study of cell targeting and delivery of oligonucleotides and their analogs. The first method involves total stepwise solid-phase synthesis on a single support. The second involves preparation of oligonucleotides containing 2'-aldehydes and subsequent chemoselective ligation with functionalized peptides to form hydrazine, oxime, or thiazolidine linkages.
Stetsenko
Dmitry A
DA
Laboratory of Molecular Biology, MRC, Cambridge, UK.
Gait
Michael J
MJ
D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*
D009841:Oligonucleotides; D010455:Peptides