Comparative evaluation of cancer chemopreventive efficacy of alpha-tocopherol and quercetin in a murine model.
Chemopreventive efficacy and the mode of action of alpha-tocopherol and quercetin, both known to possess anti-oxidant properties, were evaluated during carcinogenesis in uterine cervix of mice by chronic exposure to methylcholanthrene. Oral administration of alpha-tocopherol and quercetin could reduce the genotoxic effect of the carcinogen as revealed by the micronucleus frequency in vaginal epithelial cells. Both could restrict the progression of dysplastic changes in cervix uteri leading to carcinoma in-situ but the influence of the quercetin was more pronounced. A decrease in lipid peroxides following ingestion of these agents suggests that their chemopreventive action is due to their protective role on DNA damage by free radicals. Increased glutathione and glutathione-s-transferase also indicate their involvement in detoxification. While alpha-tocopherol activated the PBL response towards mitogenic stimulation, quercetin had no such effect.
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Dept of Cancer Chemoprevention, Chittaranjan National Cancer Institute, Kolkata, India.
Chakraborty
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Ghosh
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Sengupta
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Das
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D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000175:diagnosis / Q000517:prevention & control*; D018890:Chemoprevention; D004249:DNA Damage; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D008938:Mitosis / Q000187:drug effects; D023421:Models, Animal; D011794:Quercetin / Q000627:therapeutic use*; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced / Q000175:diagnosis / Q000517:prevention & control*; D024502:alpha-Tocopherol / Q000627:therapeutic use*
D000975:Antioxidants; D005609:Free Radicals; D008748:Methylcholanthrene; D011794:Quercetin; D005982:Glutathione Transferase; D005978:Glutathione; D024502:alpha-Tocopherol