[Comparison of chromosomal defects in primary tumor and metastases by comparative genomic hybridization (CGH)].
Molecular methods can complement the classical methods in pathology like macroscopic, histological and immunohistochemical examinations.Comparative genomic hybridization is a cytogenetic method to screen for gains and losses of chromosomal material in tumor cells. This method allows defect studies of archival paraffin-embedded and formalin-fixed tumor material. CGH can detect gains and losses of chromosomal material that are at least 10 to 20 megabases in size. This genome-wide screening method allows to study the cytogenetic relationship between differently located tumors of a patient. To answer the question if these different tumors are metastases of the primary lung tumor or independent primary tumors CGH analysis is a supplementary method that introduces new prospectives.
Simon
F
F
Institut für Pathologie, Berufsgenossenschaftliche Kliniken Bergmannsheil Bochum. simon-franky@t-online.de
Vogt
M
M
Kuhnen
C
C
Johnen
G
G
Müller
K-M
KM
D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000235:genetics*; D002877:Chromosomes, Human; D006801:Humans; D009362:Neoplasm Metastasis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000469:parasitology*; D009693:Nucleic Acid Hybridization / Q000379:methods*