Di- and oligonucleotide synthesis using H-phosphonate chemistry.
In this chapter, a concise account of the synthesis of oligonucleotides using the H-phosphonate methodology is given. It includes various methods for the preparation of the starting material, nucleoside 3'-H-phosphonate monoesters, their conversion into dinucleoside H-phosphonate diesters, oxidative transformations of dinucleoside H-phosphonates into the corresponding phosphate and phosphorothioate derivatives, and protocols for the synthesis of oligonucleotides and their phosphorothio analogs.
Stawinski
Jacek
J
Arrhenius Laboratory, Department of Chemistry, Stockholm University, Sweden.
Strömberg
Roger
R
D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry*
D009841:Oligonucleotides; D063065:Organophosphonates