title: Analysis of a conserved duplicated tRNA gene in the mitochondrial genome of blowflies.
abstract: The mtDNA control region (CR) and flanking genes of the blowflies Chrysomya albiceps, Chrysomya megacephala and Chrysomya chloropyga (Calliphoridae) were characterized. The most unusual feature found was the presence of duplicated tRNA sequences corresponding to trnI and a portion of trnQ. The partially duplicated trnQ was very likely a pseudogene since most of the sequence of the typical insect trnQ gene was missing. In contrast, the trnI gene had a conserved primary sequence following the duplication event and may represent a functional copy. These results demonstrate the plasticity of the mtDNA molecule in Chrysomya, especially for tRNA genes and the adjacent control region sequences.
authors.affiliation: Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), C.P. 6010, CEP 13083-875, Campinas, SP, Brazil. lessinge@unicamp.br
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Lessinger
authors.forename: Ana Carolina M
authors.initials: AC
authors.lastname: Junqueira
authors.forename: Fábio F
authors.initials: FF
authors.lastname: Conte
authors.forename: Ana Maria L
authors.initials: AM
authors.lastname: Azeredo-Espin
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D020131:Genes, Duplicate / Q000235:genetics*; D008969:Molecular Sequence Data; D011544:Pseudogenes / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D012351:RNA, Transfer, Gln / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004272:DNA, Mitochondrial; D012351:RNA, Transfer, Gln; D012355:RNA, Transfer, Ile; D004247:DNA; D012343:RNA, Transfer