In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells.
Human mesenchymal stem cells (hMSCs) have been paid a great deal of attention because of their unprecedented therapeutic merits endowed by powerful ex vivo expansion and multilineage differentiation potential. Umbilical cord blood (UCB) is a convenient but not fully proven source for hMSCs, and hence, greater experience is required to establish UCB as a reliable source of hMSCs. To this end, we attempted to isolate hMSC-like adherent cells from human UCB. The isolated cells were highly proliferative and exhibited an immunophenotype of CD13+ CD14- CD29+ CD31- CD34- CD44+ CD45- CD49e+ CD54+ CD90+ CD106- ASMA+ SH2+ SH3+ HLA-ABC+ HLA-DR-. More importantly, these cells, under appropriate conditions, could differentiate into a variety of mesenchymal lineage cells such as osteoblasts, chondrocytes, adipocytes, and skeletal myoblasts. This mesengenic potential assures that the UCB-derived cells are multipotent hMSCs and further implicates that UCB can be a legitimate source of hMSCs.
Gang
Eun Ji
EJ
Research Institute of Biotechnology, Histostem Co., Kangdong-Gu, Seoul, Republic of Korea.
Hong
Seung Hyun
SH
Jeong
Ju Ah
JA
Hwang
Soo Han
SH
Kim
Seong Whan
SW
Yang
Il Ho
IH
Ahn
Chiyoung
C
Han
Hoon
H
Kim
Hoeon
H
D015703:Antigens, CD / Q000097:blood; D001483:Base Sequence; D002448:Cell Adhesion; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology; D017931:DNA Primers; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D007700:Kinetics; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D015703:Antigens, CD; D017931:DNA Primers