[Serial pathologic changes in livers of Tree shrews and Macaca assamensises infected with human Hepatitis B virus].
BACKGROUND
To serially observe the pathologic changes in livers of tree shrews and macaca assamensises infected with HHBV.


METHODS
10 adult tree shrews and 28 macaca assamensises were inoculated with HBV rich human sera. The liver of the animals were regularly biopsied. The liver samples were examined histopathologically by HE staining. Some samples were stained for HBsAg by immunohistochemistry (IH), and HBV DNA by in situ hybridization (ISH).


RESULTS
HBsAg in 80% of tree shrews infected with HHBV can be detected by IH, HBV DNA in 50% of those can be found by ISH.The positive rates of HBsAg in macaca assamensises' livers were 25% by IH, none HBV DNA was detected.


CONCLUSIONS
The tree shrew model seems to be applicable for the research of human hepatitis B.
Wang
Shu-sheng
SS
Guangxi Center for Diseases Prevention and Control, Nanning 530021, China.
Su
Jian-jia
JJ
Feng
Bai-fang
BF
Li
Yuan
Y
Zhang
Tao
T
Qin
Liu-liang
LL
Huang
Guo-yong
GY
Gao
Jian-en
JE
Ge
Xian-min
XM
Li
He-min
HM
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006509:Hepatitis B / Q000276:immunology / Q000473:pathology* / Q000821:virology; D006515:Hepatitis B virus / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000473:pathology* / Q000821:virology*; D008251:Macaca; D008297:Male; D014418:Tupaiidae
D000914:Antibodies, Viral