title: [The mechanism of homocysteine in Kkay mice diabetic nephropathy model].
abstract: OBJECTIVE
  To study the expression of matrix metalloproteinase-9 (MMP-9) in diabetic nephropathy of Kkay mice with hyperhomocysteinemia.
  
  
  METHODS
  Sixteen Kkay mice were divided into two groups with 8 in each:diabetes group (KA) and methionine-diet group (KB). C57BL/6 mice were used as normal control (C57). Four months after being treated with two different diets, the mice were sacrificed and serum homocysteine (Hcy) was assayed with fluorescence polarization immunoassay. Renal pathological change was examined with periodic acid Schiff (PAS). The expression of MMP-9 protein and mRNA was detected with immunohistochemical staining and reverse transcriptase-PCR respectively.
  
  
  RESULTS
  It was shown that induction of hyperhomocysteinemia in Kkay mice (KB) with a diet enriched in methionine aggravated diabetic nephropathy as compared with the KA group (P < 0.05). The expression of MMP-9 protein and mRNA in KB group was also enhanced and the mean positive area of MMP-9 in KB group was higher than that in KA group (15.90% vs 11.14%, P < 0.05).
  
  
  CONCLUSIONS
  The increased level of Hcy could worsen the nephropathy; this may be related to a higher expression of MMP-9.
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China. lujm@medmail.com.cn
authors.forename: Ju-ming
authors.initials: JM
authors.lastname: Lu
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Guo
authors.forename: Chang-yu
authors.initials: CY
authors.lastname: Pan
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Mu
authors.forename: Xiao-man
authors.initials: XM
authors.lastname: Zou
authors.forename: Ling
authors.initials: L
authors.lastname: Yin
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Sheng
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D006711:Homocystine / Q000097:blood*; D020138:Hyperhomocysteinemia / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008715:Methionine / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D006711:Homocystine; D008715:Methionine; D020780:Matrix Metalloproteinase 9