Enantiomeric separation of cizolirtine and metabolites on amylose tris (3,5-dimethylphenyl carbamate) chiral stationary phase.
The enantiomeric resolution of (+/-)-cizolirtine, (+/-)-cizolirtine-N-oxide, (+/-)-N-desmethylcizolirtine and (+/-)-5(alpha-hydroxybenzyl)-1-methylpyrazole was achieved on amylose tris (3,5-dimethylphenyl carbamate) chiral stationary phase known as Chiralpak AD using hexane/2-propanol/triethylamine (80:20:0.05, v/v/v) as the mobile phase. The flow rate of the mobile phase used was 1.0 ml/min with UV detection at 230 nm. The values of R(s) of the resolved enantiomers of (+/-)-cizolirtine, (+/-)-cizolirtine-N-oxide, (+/-)-N-desmethylcizolirtine and (+/-)-5(alpha-hydroxybenzyl)-1-methyl pyrazole were 1.20, 0.60, 1.16 and 1.15, respectively.
Aboul-Enein
Hassan Y
HY
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC 03-65), King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia. enein@kfshrc.edu.sa
Ali
Imran
I
D000688:Amylose / Q000031:analogs & derivatives* / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D048448:Phenylcarbamates / Q000032:analysis*; D011720:Pyrazoles / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D048448:Phenylcarbamates; D011720:Pyrazoles; C080033:Chiralpak AD; D000688:Amylose; C075798:cizolirtine