title: Nuclear expression of BCL10 or nuclear factor kappa B predicts Helicobacter pylori-independent status of early-stage, high-grade gastric mucosa-associated lymphoid tissue lymphomas.
abstract: OBJECTIVE
  A high percentage of early-stage, high-grade gastric mucosa-associated lymphoid tissue (MALT) lymphomas remain Helicobacter pylori dependent. t(11;18)(q21;q21), a genetic aberration highly predictive of H. pylori-independent status in low-grade gastric MALT lymphoma, is rarely detected in its high-grade counterpart. This study examined whether nuclear expression of BCL10 or nuclear factor kappa B (NF-kappaB) is useful in predicting H. pylori-independent status in patients with stage IE high-grade gastric MALT lymphomas.
  
  
  METHODS
  Twenty-two patients who had participated in a prospective study of H. pylori eradication for stage IE high-grade gastric MALT lymphomas were studied. The expression of BCL10 and NF-kappaB in pretreatment paraffin-embedded lymphoma tissues was evaluated by immunohistochemistry and confocal immunofluorescence microscopy. The presence of t(11;18)(q21;q21) was identified by a multiplex reverse transcriptase polymerase chain reaction of the API2-MALT1 chimeric transcript.
  
  
  RESULTS
  Aberrant nuclear expression of BCL10 was detected in seven (87.5%) of eight H. pylori-independent and in none of 14 H. pylori-dependent high-grade gastric MALT lymphomas (P <.001). All seven patients with nuclear BCL10 expression had nuclear expression of NF-kappaB, compared with only two of 15 patients without nuclear BCL10 expression (P =.002). As a single variable, the frequency of nuclear expression of NF-kappaB was also significantly higher in H. pylori-independent tumors than in H. pylori-dependent tumors (seven of eight [87.5%] v two of 15 [12.3%]; P =.002). The API2-MALT1 fusion transcript was detected in only one (12.5%) of eight H. pylori-independent lymphomas.
  
  
  CONCLUSIONS
  Nuclear expression of BCL10 or NF-kappaB is highly predictive of H. pylori-independent status in high-grade gastric MALT lymphoma, and coexpression of these two markers in the nuclei is frequent.
authors.affiliation: Department of Oncology, National Taiwan University Hospital and National Taiwan University College of Medidine, Taipei, Taiwan.
authors.forename: Sung-Hsin
authors.initials: SH
authors.lastname: Kuo
authors.forename: Li-Tzong
authors.initials: LT
authors.lastname: Chen
authors.forename: Kun-Huei
authors.initials: KH
authors.lastname: Yeh
authors.forename: Ming-Shiang
authors.initials: MS
authors.lastname: Wu
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Hsu
authors.forename: Pei-Yen
authors.initials: PY
authors.lastname: Yeh
authors.forename: Tsui-Lien
authors.initials: TL
authors.lastname: Mao
authors.forename: Chi-Long
authors.initials: CL
authors.lastname: Chen
authors.forename: Shin-Lian
authors.initials: SL
authors.lastname: Doong
authors.forename: Jaw-Town
authors.initials: JT
authors.lastname: Lin
authors.forename: Ann-Lii
authors.initials: AL
authors.lastname: Cheng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D000074018:B-Cell CLL-Lymphoma 10 Protein; D015415:Biomarkers / Q000032:analysis; D005260:Female; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism* / Q000821:virology; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011446:Prospective Studies; D014178:Translocation, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D015415:Biomarkers; D016328:NF-kappa B; D009363:Neoplasm Proteins