title: Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10.
abstract: Macrophages co-incubated with Candida albicans strain CR1 in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CR1 caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL-10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat-killed C. albicans did not cause those effects. IL-10 production was low during the first 6 h post-infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CR1 with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL-10 production, suggesting that the increased production of IL-10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells.
authors.affiliation: Departamento de Ciências Patológicas, CCB, Universidade Estadual de Londrina, Cx P. 6001, 86051-990 Londrina, Brazil.
authors.forename: Thaís Helena
authors.initials: TH
authors.lastname: Gasparoto
authors.forename: Luis Carlos Jabur
authors.initials: LC
authors.lastname: Gaziri
authors.forename: Eva
authors.initials: E
authors.lastname: Burger
authors.forename: Ricardo Sérgio Couto
authors.initials: RS
authors.lastname: de Almeida
authors.forename: Ionice
authors.initials: I
authors.lastname: Felipe
mesh_terms: D000818:Animals; D017209:Apoptosis; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D016753:Interleukin-10 / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D008297:Male; D051379:Mice
chemical_list: D016753:Interleukin-10