[Interspecies nuclear transfer using nonquiescent somatic cell].
It is the point at issue in intraspecies nuclear transfer whether quiescence is necessary for development of nuclear transfer reconstructed embryos. In the interspecies nuclear transfer, some reports have proved that quiescent cell is able to support preimplantation development of the interspecies reconstructed embryos. Are non-quiescent cells able to support preimplantation development of the interspecies reconstructed embryos? We used non-quiescent somatic cells from C57BL/6 mice and giant pandas as donors to transfer into enucleated rabbit oocytes. After electrofusion (the electrofusion rates were 62.2% and 71.6%, respectively) and electrical activation, 5.1% of those mouse-rabbit reconstructed embryos developed to blastocyst in vitro, and 4.2% of panda-rabbit reconstructed embryos developed to blastocyst after transferring into ligated rabbit oviduct. These results indicate that non-quiescent cell from C57BL/6 mouse and giant panda could be dedifferentiated in enucleated rabbit oocytes and support early embryo development.
Li
Jin Song
JS
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080.
Han
Zhi Ming
ZM
Zhu
Zhi Yu
ZY
Wen
Duan Cheng
DC
Liu
Zhong Hua
ZH
Wang
Min Kang
MK
Lian
Li
L
Du
Jun
J
Wang
Peng Yan
PY
Zhang
He Min
HM
Chen
Da Yuan
DY
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D011817:Rabbits; D001503:Ursidae