title: Improved resolution power of electrophoretic fractionation of DNA using a voltage gradient "up and down" application.
abstract: The improved resolution power of electrophoretic fractionation of DNA in a wide range of molecular masses is demonstrated using an "up and down" application of voltage gradient gel electrophoresis (VGGE). This application also allows separation of different DNA fragments which are poorly fractionated in conventional electrophoresis.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Palermo, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Rosalia
authors.initials: R
authors.lastname: Sanfilippo
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sisino
authors.forename: Laura
authors.initials: L
authors.lastname: Palmeri
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D004247:DNA