Distribution and MK-801-induced expression of serine racemase mRNA in rat brain by real-time quantitative PCR.
We have used real-time quantitative PCR methods to evaluate the effect of the systemic administration of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine (MK-801; 0.4 mg/kg) on the gene expression of serine racemase in several brain areas of the rat. The levels of serine racemase mRNA in all the brain areas transiently increased after the administration. The present findings suggest that there is a link between the expression of serine racemase mRNA and the activity of the NMDA receptors.
Yoshikawa
Masanobu
M
Department of Pharmacology, School of Medicine, Tokai University, Isehara, Kanagawa 259-1143, Japan.
Kobayashi
Tomomi
T
Oka
Tetsuo
T
Kawaguchi
Mitsuru
M
Hashimoto
Atsushi
A
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*; D019998:Racemases and Epimerases / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D000099300:Serine Racemase
D016291:Dizocilpine Maleate; D018691:Excitatory Amino Acid Antagonists; D012333:RNA, Messenger; D019998:Racemases and Epimerases; D016194:Receptors, N-Methyl-D-Aspartate; D000099300:Serine Racemase