title: Optimal fermentation conditions for enhanced glutathione production by Saccharomyces cerevisiae FF-8.
abstract: The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
authors.affiliation: Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Cha
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Beong-Sam
authors.initials: BS
authors.lastname: Jeon
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Cho
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D003545:Cysteine / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism; D017680:Potassium Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry
chemical_list: D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; C013216:potassium phosphate; D005978:Glutathione; D005947:Glucose; D003545:Cysteine