title: Di- and oligonucleotide synthesis using H-phosphonate chemistry.
abstract: In this chapter, a concise account of the synthesis of oligonucleotides using the H-phosphonate methodology is given. It includes various methods for the preparation of the starting material, nucleoside 3'-H-phosphonate monoesters, their conversion into dinucleoside H-phosphonate diesters, oxidative transformations of dinucleoside H-phosphonates into the corresponding phosphate and phosphorothioate derivatives, and protocols for the synthesis of oligonucleotides and their phosphorothio analogs.
authors.affiliation: Arrhenius Laboratory, Department of Chemistry, Stockholm University, Sweden.
authors.forename: Jacek
authors.initials: J
authors.lastname: Stawinski
authors.forename: Roger
authors.initials: R
authors.lastname: Strömberg
mesh_terms: D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D063065:Organophosphonates