title: Molecular cloning of bovine eIF5A and deoxyhypusine synthase cDNA.
abstract: Deoxyhypusine synthase is the first of the two enzymes that catalyzes the maturation of eukaryotic initiation factor 5A (eIF5A). The mature eIF5A is the only known protein in eukaryotic cells that contains the unusual amino acid hypusine (N(epsilon)-(4-amino-2(R)-hydroxybutyl)-lysine). Synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival. Here we describe the cloning and characterization of bovine eIF5A and bovine deoxyhypusine synthase. The deduced bovine eIF5A protein is 100% identical to human eIF5A-1, and the deduced bovine deoxyhypusine synthase protein showed a 93% identity to the human protein.
authors.affiliation: Department of Chemistry, One University Circle, Western Illinois University, Macomb, IL 61455, USA.
authors.forename: Jenq-Kuen
authors.initials: JK
authors.lastname: Huang
authors.forename: Shuhui
authors.initials: S
authors.lastname: Tsai
authors.forename: George H
authors.initials: GH
authors.lastname: Huang
authors.forename: Valerie C
authors.initials: VC
authors.lastname: Sershon
authors.forename: Adrienne M
authors.initials: AM
authors.lastname: Alley
authors.forename: Lisa
authors.initials: L
authors.lastname: Wen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D018076:DNA, Complementary; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C063507:deoxyhypusine synthase