[Expression and significance of estrogen receptor isoforms in the development of human breast carcinoma].
OBJECTIVE
To investigate the estrogen receptors (ER)alpha and ERbeta expression and their relationship with clinicopathological parameters in human breast carcinoma.


METHODS
Samples were obtained from 30 breast carcinoma, reverse transcriptase polymerase chain reaction was used to measure the expression of ERalpha and ERbeta mRNA.


RESULTS
ERalpha mRNA level was up-regulated in breast carcinoma tissue compared with adjacent normal tissue (t = 7.399, P < 0.01) while down-regulated in ERbeta. The relative ratio of ERalpha and ERbeta was decreased in normal tissue vs. carcinoma (t = 6.385, P < 0.01), in patients with lymph node metastasis vs. those without lymph node metastasis (t = 2.602, P < 0.05), in late stage carcinoma vs. early stage (t = 3.754, P < 0.05).


CONCLUSIONS
ERalpha and ERbeta play divergent role in the development of human breast carcinoma.
Wang
Shu
S
Breast Disease Center, Peking University People's Hospital, Beijing 100044, China.
Wang
Shan
S
Zhang
Jia-qing
JQ
Qiao
Xin-min
XM
Yang
De-qi
DQ
Tong
Fu-zhong
FZ
Ye
Ying-jiang
YJ
Cui
Zhi-rong
ZR
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D047629:Estrogen Receptor beta / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger