title: 4-hydroxy-2,3 trans-nonenal nuclear concentration inversely correlates with the growth rate of T leukemia cells.
abstract: 4-hydroxy-2,3 trans-nonenal is the major diffusible product generated by linoleic and arachidonic acids peroxidation. Its endogenous content is inversely related to the rate of cell proliferation and directly related to the level of cell differentiation. As previously reported, the nuclear localization of the aldehyde has been observed by means of a fluorescent antibody and confocal microscopy, and its concentration measured by electrospray/mass spectrometry, a sensitive and selective method for 4-hydroxy-2,3 trans-nonenal determination, on nuclear extracts of leukemic cells. With the aim to establish a possible correlation between the peroxidation product nuclear concentration and cell growth rate, Jurkat 6 leukemic cells have been used and the aldehyde measured by electrospray/mass spectrometry. The cells arrested in G1 show a content of 4-hydroxy-2,3 trans-nonenal significantly increased with respect to control ones.
authors.affiliation: Department of Biochemistry "G. Moruzzi", University of Bologna, Bologna, Italy.
authors.forename: Natalia
authors.initials: N
authors.lastname: Calonghi
authors.forename: Concettina
authors.initials: C
authors.lastname: Cappadone
authors.forename: Eleonora
authors.initials: E
authors.lastname: Pagnotta
authors.forename: Carla
authors.initials: C
authors.lastname: Boga
authors.forename: Carlo
authors.initials: C
authors.lastname: Bertucci
authors.forename: Jessica
authors.initials: J
authors.lastname: Fiori
authors.forename: Pietro
authors.initials: P
authors.lastname: Traldi
authors.forename: Lanfranco
authors.initials: L
authors.lastname: Masotti
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry*; D049109:Cell Proliferation; D006801:Humans; D019169:Jurkat Cells; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000447:Aldehydes; C027576:4-hydroxy-2-nonenal