title: Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions.
abstract: We have characterized the modulation of cell-cell adhesion and the structure of adherens junctions in the human colon adenocarcinoma HT-29 cell line that differentiates into enterocytes after glucose substitution for galactose in the medium. We demonstrate that differentiated cells (HT-29 Gal) rapidly established E-cadherin-mediated interactions in aggregation assays. This effect is not due to an increase in E-cadherin expression during this early stage of cell differentiation, but rather results from the maturation of preexisting adherens junctions. These junctions are characterized by the redistribution of E-cadherin to the basolateral membrane and its co-localization with the actin cytoskeleton. Subcellular fractionation studies indicate that actin-associated E-cadherins bind beta-catenin and p120ctn. Furthermore, the p120ctn/E-cadherin association is upregulated. These data reveal a cooperative interaction between p120ctn and E-cadherin that corresponds to mature functional adherens junctions able to initiate tight cell-cell adhesion required for epithelium architecture and further affirm the gatekeeper role of p120ctn.
authors.affiliation: Laboratoire d'Etude de la Différenciation et de l'Adhérence Cellulaires, UMR UJF/CNRS 5538, Institut Albert Bonniot, Faculté de Médecine de Grenoble, 38706 La Tronche Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Gout
authors.forename: C
authors.initials: C
authors.lastname: Marie
authors.forename: M
authors.initials: M
authors.lastname: Lainé
authors.forename: G
authors.initials: G
authors.lastname: Tavernier
authors.forename: M R
authors.initials: MR
authors.lastname: Block
authors.forename: M
authors.initials: M
authors.lastname: Jacquier-Sarlin
mesh_terms: D000199:Actins; D022005:Adherens Junctions / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D051177:Catenins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D016764:Cell Polarity; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton; D020895:Enterocytes / Q000378:metabolism*; D005947:Glucose / Q000172:deficiency; D019073:HT29 Cells; D006801:Humans; D007365:Intercellular Junctions; D010750:Phosphoproteins / Q000378:metabolism*; D013347:Subcellular Fractions; D015534:Trans-Activators / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051176:beta Catenin; D000095002:Delta Catenin
chemical_list: D000199:Actins; C495270:CTNNB1 protein, human; D015820:Cadherins; D051177:Catenins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D015534:Trans-Activators; D051176:beta Catenin; D005947:Glucose; D000095002:Delta Catenin; C000722347:CTNND1 protein, human