Divergent effects of caloric restriction on gene expression in normal and long-lived mice.
Long-lived Ames dwarf mice share many phenotypic characteristics with animals subjected to caloric restriction (CR) but they are not CR mimetics. CR prolongs longevity in both normal and Ames dwarf mice. Using real-time polymerase chain reaction and western blot, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice subjected to 30% CR. The results revealed divergent responses of dwarf and normal animals to CR raising an interesting possibility that CR may affect longevity of normal and dwarf mice by different mechanisms. Moreover, effects of dwarfism on the expression of the examined genes differed from the effects of CR, thus adding to the evidence that these long-lived mutants are not CR mimetics.
Masternak
Michal M
MM
Department of Internal Medicine, Southern Illinois University, School of Medicine, Springfield 62794-9628, USA. mmasternak@siumed.edu
Al-Regaiey
Khalid
K
Bonkowski
Michael S
MS
Panici
Jacob
J
Sun
Liou
L
Wang
Jian
J
Przybylski
Grzegorz K
GK
Bartke
Andrzej
A
D000818:Animals; D015153:Blotting, Western; D031204:Caloric Restriction; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D007328:Insulin / Q000502:physiology; D008136:Longevity / Q000502:physiology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D016133:Polymerase Chain Reaction
D007328:Insulin