[Application of enzyme histochemistry in evaluation of in vitro and in vivo biocompatibility of HA/TCP].
In order to explore the possibility of applying enzyme histochemistry in biocompatibility evaluation, we investigated the effect of biomaterials on the activities of intracellular enzymes in this experiment. It was found that there was no obvious difference in morphology between osteoblasts co-cultured with HA/TCP and with Ti-alloy. However, transient down-regulation of NADH, SDH, LDH and CCO of the osteoblasts co-cultured with HA/TCP was detected by enzyme histochemistry, but these enzymes of osteoblasts the co-cultured with Ti-alloy were not down-regulated. It was indicated that something extracted from HA/TCP injured the co-cultured osteoblasts slightly. Similar early acute inflammatory reactions were observed after HA/TCP and Ti-alloy were separately implanted into the dorsal muscle of rabbit. There was also no obvious difference between the tissue response to HA/TCP and that to Ti-alloy. Activities of enzymes in tissues around implanted materials were down-regulated at the early injury period and recovered gradually within 30 days post-operation. But the mild toxicity of extracts from HA/TCP was demonstrated by the fact that the recovery period of HA/TCP group was longer than that of Ti-alloy group. It was proved that enzyme histochemistry is more sensitive than tissue morphology analysis in detecting the cell or tissue responses to biomaterials. Therefore, it is possible to use enzyme histochemistry in biocompatibility evaluation.
Lu
Bing
B
Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
Lu
Xiaofeng
X
Zhang
Zhen
Z
Li
Shengfu
S
Pei
Fuxing
F
Li
Youping
Y
Cheng
Jingqiu
J
D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D018920:Coculture Techniques; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006882:Hydroxyapatites / Q000737:chemistry*; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology*; D011817:Rabbits; D014025:Titanium
D000497:Alloys; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; D014025:Titanium; C018392:tricalcium phosphate