Geranylgeranylacetone attenuates suppression by Helicobacter pylori extract of human umbilical vein epithelial cell growth.
OBJECTIVE
Helicobacter pylori infection delays gastric ulcer healing. Angiogenesis is important for the healing of gastric ulcers. Therefore, the effects of H. pylori water extract and a novel antiulcer drug, geranylgeranylacetone, on the viability of human umbilical vein epithelial cells (HUVECs) were investigated.


METHODS
H. pylori (ATCC43504) was prepared by sonication. The HUVEC viability after treatment with H. pylori water extract alone or in combination with geranylgeranylacetone was estimated by an MTT assay.


RESULTS
H. pylori water extract significantly decreased cell viability in a concentration-dependent manner after 48 h. However, combined use of H. pylori water extract and geranylgeranylacetone significantly increased the HUVEC viability over that of H. pylori extract alone.


CONCLUSIONS
A novel antiulcer drug, geranylgeranylacetone, attenuates the H. pylori-induced inhibition of angiogenesis.
Tatsuta
Masaharu
M
Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases Osaka, Japan.
Iishi
Hiroyasu
H
Baba
Miyako
M
Iseki
Kazushige
K
D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D008833:Microcirculation / Q000187:drug effects; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D014471:Umbilical Veins / Q000166:cytology; D014508:Urea / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D000942:Antigens, Bacterial; D004224:Diterpenes; D014508:Urea; C031049:geranylgeranylacetone