Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens.
Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.
Lemaître
Nadine
N
Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire de Lille, France. n-lemaitre@chru-lille.fr
Armand
Sylvie
S
Vachée
Anne
A
Capilliez
Odile
O
Dumoulin
Christine
C
Courcol
René J
RJ
D006801:Humans; D008168:Lung / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*