Effects of phosphorylation by protein kinase CK2 on the human basal components of the RNA polymerase II transcription machinery.
We have investigated the role of phosphorylation by vertebrate protein kinase CK2 on the activity of the General Transcription Factors TFIIA, TFIIE, TFIIF, and RNAPII. The largest subunits of TFIIA, TFIIE, and TFIIF were phosphorylated by CK2 holoenzyme. Also, RNA polymerase II was phosphorylated by CK2 in the 214,000 and 20,500 daltons subunits. Our results show that phosphorylation of TFIIA, TFIIF, and RNAPII increase the formation of complexes on the TATA box of the Ad-MLP promoter. Also, phosphorylation of TFIIF increases the formation of transcripts, where as phosphorylation of RNA polymerase II dramatically inhibits transcript formation. Furthermore, we demonstrate that CK2 beta directly interacts with RNA polymerase II, TFIIA, TFIIF, and TBP. These results strongly suggest that CK2 may play a role in regulating transcription of protein coding genes.
Cabrejos
María Eugenia
ME
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile.
Allende
Catherine C
CC
Maldonado
Edio
E
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035561:Transcription Factor TFIIA / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism*; D014158:Transcription, Genetic
D060588:LIM Domain Proteins; D009124:Muscle Proteins; D035181:TATA-Box Binding Protein; D035561:Transcription Factor TFIIA; D019473:Transcription Factors, TFII; C090114:cysteine and glycine-rich protein 3; C066644:transcription factor TFIIE; D005982:Glutathione Transferase; D047390:Casein Kinase II; D012319:RNA Polymerase II; C056604:transcription factor TFIIF