Towards a miniaturised system for dynamic field gradient focused separation of proteins.
Separation and focusing of proteins is described in a miniaturised dynamic field gradient focusing device with a 2.5 cm x 0.1 cm channel filled with a porous polymer monolith. The separation channel is in contact with a parallel electric field channel with five individually addressable electrodes through a porous glass membrane so that a variable field can be generated that drives charged proteins electroosmotically against a constant hydrodynamic flow. Separated pre-stained proteins were detected by means of a digital camera and background subtraction.
Myers
P
P
Department of Chemistry, University of Leeds, Leeds LS2 9JT, UK. petermyers@scientist.com
Bartle
K D
KD
D008904:Miniaturization; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins