title: Afadin- and alpha-actinin-binding protein ADIP directly binds beta'-COP, a subunit of the coatomer complex.
abstract: Afadin DIL domain-interacting protein (ADIP) is a novel protein that binds both afadin and alpha-actinin and localizes at adherens junctions, which are formed by nectins and cadherins, cell-cell adhesion molecules. Afadin is an actin filament (F-actin)-binding protein which connects nectins to the actin cytoskeleton. alpha-Actinin is another F-actin-binding protein that is indirectly associated with cadherins through the catenin complex. ADIP is at least partly involved in the physical association of nectins and cadherins. We show here that ADIP furthermore binds beta'-COP, a subunit of the coatomer complex. ADIP co-localizes with beta'-COP at the Golgi complex in Madin Darby canine kidney and normal rat kidney cells. These results suggest that ADIP is involved in vesicle trafficking from the Golgi to the endoplasmic reticulum and through the Golgi complex by interacting with the coatomer complex.
authors.affiliation: Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Asada
authors.forename: Kenji
authors.initials: K
authors.lastname: Irie
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D020756:Coatomer Protein / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins; D011485:Protein Binding; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D020756:Coatomer Protein; D008840:Microfilament Proteins; C472296:Ssx2ip protein, rat