Human cell proteins and human immunodeficiency virus DNA integration.
Integration, catalyzed by the viral integrase (IN) protein, is a crucial step in the life cycle of all retroviruses including human immunodeficiency virus type 1 (HIV-1). Although purified HIV-1 IN protein is sufficient to catalyze the DNA breakage and joining steps of integration in the absence of any other protein factor, a number of studies indicate that cellular proteins participate in the integration process in cells. These host cell proteins have been proposed to act through binding the pre-integrated viral cDNA substrate, by directly interacting with the IN protein, and/or by repairing the single-stranded DNA gaps that occur at viral/chromosomal DNA junctions during integration. In this paper we summarize the identification and potential roles of specific cell factors in HIV-1 integration. We also present experimental results of human cell proteins that coimmunoprecipitated with HIV-1 IN following its expression in HeLa cells and discuss these results in light of the previously-identified integration cofactors.
Turlure
Fanny
F
Department of Pathology, Harvard Medical School, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.
Devroe
Eric
E
Silver
Pamela A
PA
Engelman
Alan
A
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D004260:DNA Repair; D018076:DNA, Complementary / Q000378:metabolism; D006678:HIV / Q000378:metabolism*; D015658:HIV Infections / Q000473:pathology*; D019427:HIV Integrase / Q000502:physiology*; D006367:HeLa Cells / Q000821:virology*; D006801:Humans; D011485:Protein Binding; D011995:Recombination, Genetic; D016662:Virus Integration
D018076:DNA, Complementary; D004247:DNA; D019427:HIV Integrase