Galanin inhibits gastric acid secretion through a somatostatin-independent mechanism in mice.
The role of somatostatin in galanin-induced inhibition of gastric acid secretion in urethane-anesthetized mice was investigated by using immunoneutralization of endogenous somatostatin and somatostatin receptor type 2 (SSTR2) knockout mice. Intravenous galanin (10 and 20 microg/kg/h) inhibited pentagastrin-stimulated gastric acid secretion by 47 and 33%, respectively. Somatostatin antibody injected i.v. increased acid secretion by 3.5-fold over basal levels but did not modify the antisecretory effects of galanin. Urethane-anesthetized SSTR2 knockout mice had a basal secretion 14-fold higher than wild-type animals, that was inhibited by galanin (10 and 20 microg/kg/h) by 49 and 31% respectively. In mice galanin inhibits gastric acid secretion through a somatostatin-independent mechanism.
Piqueras
Laura
L
Department of Physiology, Pharmacology and Toxicology, Cardenal Herrera CEU University, Valencia, Spain.
Taché
Yvette
Y
Martinez
Vicente
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology*; D019004:Galanin / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010418:Pentagastrin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007328:Insulin; D013004:Somatostatin; D019004:Galanin; D010418:Pentagastrin