[Compatibility research of self-designed scaffold biomaterials of nature extracellular matrix].
OBJECTIVE
Scaffold biomaterials for cell culture of tissue-engineering demand satisfactory biocompatibility. This experiment aimed to evaluate biocompatibility of self-designed scaffold biomaterial of NECM.


METHODS
The biocompatibility of self-designed NECM was evaluated both in vivo and in vitro, including cytotoxic test, acute and subacute systemic toxicity test, hemolysis test, pyrogenic test, lymphocyte transformation test and long-term subcutaneous implantation test of NECM.


RESULTS
Self-designed NECM scaffold biomaterial for cell culture has no cytotoxicity, no acute and subacute systemic toxicity, and it did not cause inflammatory reaction.


CONCLUSIONS
Self-designed NECM has good biocompatibility and may become an ideal biological scaffold material for cell culture of tissue engineering.
Wang
Yanjun
Y
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Kong
Weijia
W
Bi
Shengbin
S
D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000166:cytology; D005109:Extracellular Matrix; D006650:Histocompatibility Testing; D066298:In Vitro Techniques; D051379:Mice; D011817:Rabbits
D001672:Biocompatible Materials