title: A catalog of human cDNA expression clones and its application to structural genomics.
abstract: We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.
authors.affiliation: Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany. buessow@molgen.mpg.de
authors.forename: Konrad
authors.initials: K
authors.lastname: Büssow
authors.forename: Claudia
authors.initials: C
authors.lastname: Quedenau
authors.forename: Volker
authors.initials: V
authors.lastname: Sievert
authors.forename: Janett
authors.initials: J
authors.lastname: Tischer
authors.forename: Christoph
authors.initials: C
authors.lastname: Scheich
authors.forename: Harald
authors.initials: H
authors.lastname: Seitz
authors.forename: Brigitte
authors.initials: B
authors.lastname: Hieke
authors.forename: Frank H
authors.initials: FH
authors.lastname: Niesen
authors.forename: Frank
authors.initials: F
authors.lastname: Götz
authors.forename: Ulrich
authors.initials: U
authors.lastname: Harttig
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D002377:Catalogs as Topic; D003001:Cloning, Molecular / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D018076:DNA, Complementary / Q000096:biosynthesis*; D030541:Databases, Genetic; D015870:Gene Expression / Q000235:genetics; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011237:Predictive Value of Tests; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D012995:Solubility
chemical_list: D018076:DNA, Complementary; D010446:Peptide Fragments; D011994:Recombinant Proteins