title: The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development.
abstract: The CCCH tandem zinc finger protein, Zfp36l2, like its better-known relative tristetraprolin (TTP), can decrease the stability of AU-rich element-containing transcripts in cell transfection studies; however, its physiological importance is unknown. We disrupted Zfp36l2 in mice, resulting in decreased expression of a truncated protein in which the N-terminal 29 amino acids had been deleted (DeltaN-Zfp36l2). Mice derived from different clones of ES cells exhibited complete female infertility, despite evidence from embryo and ovary transplantation experiments that they could gestate and rear wild-type young. DeltaN-Zfp36l2 females apparently cycled and ovulated normally, and their ova could be fertilized; however, the embryos did not progress beyond the two-cell stage of development. These mice represent a specific model of disruption of the earliest stages of embryogenesis, implicating Zfp36l2, a probable mRNA-binding and destabilizing protein, in the physiological control of female fertility at the level of early embryonic development. This newly identified biological role for Zfp36l2 may have implications for maternal mRNA turnover in normal embryogenesis, and conceivably could be involved in some cases of unexplained human female infertility.
authors.affiliation: Laboratory of Signal Transduction, National Institute of Environmental Health Science, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Silvia B V
authors.initials: SB
authors.lastname: Ramos
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Stumpo
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Kennington
authors.forename: Ruth S
authors.initials: RS
authors.lastname: Phillips
authors.forename: Cheryl B
authors.initials: CB
authors.lastname: Bock
authors.forename: Fernando
authors.initials: F
authors.lastname: Ribeiro-Neto
authors.forename: Perry J
authors.initials: PJ
authors.lastname: Blackshear
mesh_terms: D000818:Animals; D002678:Chimera / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D004624:Embryo Transfer; D005091:Exons; D005260:Female; D005298:Fertility / Q000235:genetics / Q000502:physiology*; D047109:Fetal Development / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D018390:Gene Targeting; D017874:Immediate-Early Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D007438:Introns; D051379:Mice; D018345:Mice, Knockout; D010053:Ovary / Q000637:transplantation; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051816:Tristetraprolin; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D051816:Tristetraprolin; C497553:Zfp36 protein, mouse