title: The ABC's (and XYZ's) of peptide sequencing.
abstract: Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key - but little understood - technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.
authors.affiliation: Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology; D016208:Databases, Factual; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000295:instrumentation / Q000379:methods*
chemical_list: D010455:Peptides; D020033:Protein Isoforms