Comparison of necrosis in human ovarian tissue after conventional slow freezing or vitrification and transplantation in ovariectomized SCID mice.
This paper examines and compares necrosis in human ovarian tissue after conventional slow freezing or vitrification and ensuing xenotranplantation. Slow cryoconserved or vitrified ovarian tissue samples and fresh controls from nine patients were subcutaneously transplanted into SCID mice. The tissue samples were explanted after 6 weeks and the necrotic areas were examined by staining with Lucifer yellow SV. The size of the necrotic areas in parallel cultivated ovarian tissue samples was compared, as was necrosis in cultivated prostate tumour spheroids where the emergence of necrosis and its pathophysiological correlation have been described. Examinations showed no significant rise in the proportion of necrotic areas after slow cryoconservation/transplantation and in the controls (transplanted fresh tissue, not transplanted fresh tissue, long-term culture). The proportion of necrotic areas in the tumour spheroids was significantly higher than in the ovarian tissue. Vitrification could, after these results, be presented as an alternative to conventional slow cryoconservation.
Rahimi
G
G
Department of Obstetrics and Gynaecology, University of Cologne, Kerpener Strasse 34, 50931 Cologne, Germany. shiva7de@yahoo.com
Isachenko
E
E
Isachenko
V
V
Sauer
H
H
Wartenberg
M
M
Tawadros
S
S
Hescheler
J
J
Mallmann
P
P
Nawroth
F
F
D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009336:Necrosis; D016377:Organ Transplantation / Q000379:methods*; D009930:Organic Chemicals; D010053:Ovary / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology; D013997:Time Factors; D014183:Transplantation, Heterologous
D005456:Fluorescent Dyes; D007546:Isoquinolines; D009930:Organic Chemicals; C402795:SYTOX Green; C017475:lucifer yellow