title: Various immunization protocols for an acute motor axonal neuropathy rabbit model compared.
abstract: Various ganglioside immunization protocols were examined to refine the procedure for establishing an animal model of acute motor axonal neuropathy. The most effective was subcutaneous injection of an emulsion of 2.5mg of bovine brain ganglioside mixtures, keyhole lympet hemocyanin, and complete Freund's adjuvant to Japanese white rabbits, repeated at 3-week intervals. Under that protocol, all the rabbits developed marked flaccid paralysis associated with plasma anti-GM1 IgG antibody. This acute motor axonal neuropathy rabbit model also could be reproduced by the use of incomplete Freund's adjuvant, methylated bovine serum albumin, and New Zealand white rabbits. These results provide useful information for the confirmation of and further research on the model.
authors.affiliation: Department of Neurology, Dokkyo University School of Medicine, Kitakobayashi 880, Mibu, Shimotsuga, Tochigi 321-0293, Japan. ksusuki@dokkyomed.ac.jp
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Susuki
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishimoto
authors.forename: Michiaki
authors.initials: M
authors.lastname: Koga
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagashima
authors.forename: Izumi
authors.initials: I
authors.lastname: Mori
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirata
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yuki
mesh_terms: D000208:Acute Disease; D000818:Animals; D020274:Autoimmune Diseases of the Nervous System / Q000276:immunology / Q000517:prevention & control*; D001369:Axons / Q000276:immunology*; D001500:BCG Vaccine / Q000627:therapeutic use; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D005732:Gangliosides / Q000276:immunology; D016537:Gangliosidosis, GM1 / Q000276:immunology / Q000517:prevention & control; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization; D008297:Male; D009046:Motor Neurons / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D005732:Gangliosides; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin