Presynaptic dopaminergic properties of differentiated mouse embryonic stem cells.
This study characterized the presynaptic dopaminergic properties of neuronally differentiated mouse embryonic stem (ES) cells. Approximately 30% of the ES cells expressed tyrosine hydroxylase (TH) immunoreactivity when co-cultured with PA6 cells. These cultures expressed high affinity, sodium-dependent dopamine uptake as well as depolarization-induced and calcium-dependent dopamine release of this transmitter. These and other important dopaminergic genes found expressed in these cultures by RT-PCR included Nurr1, vesicular monoamine transporter 2 (VMAT2), TH, dopamine transporter (DAT), and glial cell line-derived neurotrophic factor (GDNF) receptors c-Ret and GFRalpha1. These results demonstrate that differentiated ES cells have the presynaptic functions for maintaining dopaminergic homeostasis, which may be essential for their long-term use in restoring CNS levels of this transmitter.
Nakano
Y
Y
Department of Pharmacology, College of Pharmacy, University of Florida, P.O. Box 100267, 1600 SW Archer, Gainesville, FL 32610, USA.
Hirko
A C
AC
Smith
A D
AD
Oka
M
M
Dawson
R
R
Peris
J
J
Terada
N
N
Meyer
E M
EM
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D004298:Dopamine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004622:Embryo, Mammalian; D051379:Mice; D017729:Presynaptic Terminals / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D004298:Dopamine