Trypanosoma cruzi: TcRAB7 protein is localized at the Golgi apparatus in epimastigotes.
In mammalian cells, the Rab7 protein is a key element of late endocytic membrane traffic. Several results suggest that it is involved in the transport from early to late endosome or from late endosome to lysosome. We have previously characterized a Rab7 gene homologue (TcRAB7) in Trypanosoma cruzi. Now, using an affinity-purified antibody specific to TcRAB7 protein we have determined that it is localized at the Golgi apparatus of the parasite. Our results indicate that the T. cruzi Rab7 homologue may function in a different route than its counterparts in mammalian cells.
Araripe
Júlia R
JR
Lab. Metabolismo Macromolecular Firmino Torres de Castro, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Cunha e Silva
Narcisa L
NL
Leal
Simone T
ST
de Souza
Wanderley
W
Rondinelli
Edson
E
D000818:Animals; D006056:Golgi Apparatus / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D021381:Protein Transport; D014349:Trypanosoma cruzi / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D007106:Immune Sera; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins