title: Gene expression profiles of three different models of reconstructed human epidermis and classical cultures of keratinocytes using cDNA arrays.
abstract: The gene expression profiles of three different models of reconstructed human epidermis were analyzed in a comparative study using cDNA array technology. The study also included normal human subconfluent keratinocytes cultured on plastic. Arrays were custom-made and comprised 504 known genes related to cutaneous biology. The gene expression profiles of the three reconstructed epidermis models shared 86% similarity; only 22 of the 504 examined genes showed a different expression level. A comparison of the 3D models with keratinocyte cultures on plastic dishes revealed a set of six genes with a considerably higher expression in the 3D models. These genes were keratin 1, corneodesmosin, filaggrin, loricrin, calmodulin-like skin protein and caspase 14, all related to keratinocyte terminal differentiation. The reported data may contribute to a better understanding and characterization of reconstructed epidermal models and may also serve as established references for investigations related to epidermal differentiation and proliferation.
authors.affiliation: L'Oréal, Centre Charles Zviak, 90 rue du Général Roguet, 92583 Clichy, France.
authors.forename: B
authors.initials: B
authors.lastname: Méhul
authors.forename: D
authors.initials: D
authors.lastname: Asselineau
authors.forename: D
authors.initials: D
authors.lastname: Bernard
authors.forename: J
authors.initials: J
authors.lastname: Leclaire
authors.forename: M
authors.initials: M
authors.lastname: Régnier
authors.forename: R
authors.initials: R
authors.lastname: Schmidt
authors.forename: F
authors.initials: F
authors.lastname: Bernerd
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003584:Cytological Techniques; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D000091344:Filaggrin Proteins; D020869:Gene Expression Profiling; D006801:Humans; D007158:Immunologic Techniques; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010969:Plastics; D013194:Staining and Labeling; D023822:Tissue Engineering
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D010969:Plastics