Determination of serotonin and its precursors in human plasma by capillary electrophoresis-electrospray ionization-time-of-flight mass spectrometry.
A specific capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) method for the determination of serotonin (5HT) and its precursors tryptophan (Trp) and 5-hydroxytryptophan (5HTP) in human platelet rich plasma is described. The analytes were removed from the plasma samples and preconcentrated by solid phase extraction (SPE) on mixed mode cation-exchange sorbents. The SPE recoveries were 71.6 +/- 3.1 for 5HT, 91.0 +/- 2.8 for Trp, and 95.3 +/- 5.9% for 5HTP. Deuterated analogues of 5HT and Trp were used as internal standards for quantitation purposes. Submicromolar detection limits were obtained for standard mixtures of all compounds and their deuterated isotopes, except 5HTP, which had detection limits in the low micromolar range. The potential usefulness of this method in the clinical setting was demonstrated by analyzing plasma extracts from healthy volunteers as well as from pathological samples. While 5HTP was not present in any of the analyzed samples, the levels of 5HT and Trp in both normal and pathological plasma were determined.
Peterson
Zlatuse D
ZD
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, USA.
Lee
Milton L
ML
Graves
Steven W
SW
D006916:5-Hydroxytryptophan / Q000097:blood; D000465:Algorithms; D001792:Blood Platelets / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary; D006801:Humans; D007202:Indicators and Reagents; D011522:Protons; D012015:Reference Standards; D015203:Reproducibility of Results; D012701:Serotonin / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000097:blood
D002021:Buffers; D007202:Indicators and Reagents; D011522:Protons; D012701:Serotonin; D014364:Tryptophan; D006916:5-Hydroxytryptophan