Formation and dispersion of mycelial pellets of Streptomyces coelicolor A3(2).
The pellets from a culture of Streptomyces coelicolor A3(2) that were submerged shaken were disintegrated into numerous hyphal fragments by DNase treatment. The pellets were increasingly dispersed by hyaluronidase treatment, and mycelial fragments were easily detached from the pellets. The submerged mycelium grew by forming complexes with calcium phosphate precipitates or kaolin, a soil particle. Therefore, the pellet formation of Streptomyces coelicolor A3(2) can be considered a biofilm formation, including the participation of adhesive extracellular polymers and the insoluble substrates.
Kim
Yul-Min
YM
Department of Microbiology, Dankook University, Anseo-Dong San 29, Cheonan 330-714, Korea.
Kim
Jae-heon
JH
D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D002130:Calcium Phosphates / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D007616:Kaolin / Q000378:metabolism; D025282:Mycelium / Q000166:cytology; D013302:Streptomyces / Q000254:growth & development*
D002130:Calcium Phosphates; D007616:Kaolin; C020243:calcium phosphate; D003851:Deoxyribonucleases; D006821:Hyaluronoglucosaminidase