[DNA quantification in nuclei of cultivated mushroom with DAPI staining].
Agaricus bisporus (Lange) Imbach is actively cultivated amphithallic basidiomycete, in which various strains are primary homothallic, heterothallic or secondary homothallic. Countings of relative nuclear DNA content by means of DAPI stain and its comparison in different strains can help to understand the mushroom's life cycle features. The authors for the first time observed change of nuclear phases in basidia of A. bisporus strains with different types of life cycle and revealed that DNA content in diploid nuclei is about 1.3 times higher than in haploid ones. The method is highly sensitive and can be used for quantitative measurings of nuclear DNA even in objects with nuclei of about 1 mkm in diameter.
Pancheva
E V
EV
Volkova
V N
VN
Kamzolkina
O V
OV
D000364:Agaricus / Q000737:chemistry / Q000235:genetics*; D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry; D004271:DNA, Fungal / Q000032:analysis*; D005456:Fluorescent Dyes; D007211:Indoles; D013194:Staining and Labeling
D004271:DNA, Fungal; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI