[The ultracytochemical localization of ATPase activity in pollen tube and stigma of Fagopyrum esculentum after compatible and incompatible pollination].
Ultracytochemical localization of adenosine triphosphatase (ATPase) activity in stigmas, pollens and pollen tubes of Fagopyrum esculentum was performed with the cytochemical method of lead phosphate precipitation. The results were as follows: (1) Lower activities of ATPase appeared in stigma cells at 0.5 hour after compatible and incompatible pollination. Stigma surface and pollen grains attached on stigma showed higher ATPase activities after compatible pollination and lower or no activities after incompatible pollination at 0.5 hour. ATPase localized on endoplasmic reticula and sperm cell in pollen grain. (2) Lower ATPase activities appeared both in stigma cells and pollen tubes in style at 1.5 hours after incompatible pollination. Pollen tube stopped growing and the degeneration of its cytoplasma began; on the contrary, at 1.5 hours after compatible pollination, higher ATPase activities were detected both in stigma cells and pollen tube in style. ATPase localized mainly on plasmolemma, in cytoplasmic matrix of stigma cells and in mitochondria, dictyosome, plastid envelop, and on the wall of pollen tube as well. The present study indicated that the stop of pollen tube growth after self-incompatibility of Fagopyrum esculentum resulted not only from the nutrient starvation of pollen tube, but also from its metabolic disorder in incompatible style.
Gao
Xin Qi
XQ
Department of Biology, Qufu Normal University, Shandong Qufu 273165.
Wang
Xiu Ling
XL
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D019612:Fagopyrum / Q000201:enzymology* / Q000502:physiology* / Q000648:ultrastructure; D035264:Flowers / Q000201:enzymology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000378:metabolism*; D053205:Pollen Tube / Q000201:enzymology* / Q000648:ultrastructure; D054817:Pollination / Q000502:physiology*
D010940:Plant Proteins; D000251:Adenosine Triphosphatases