title: Evaluation of a ceftiofur-washed whole cell Streptococcus suis bacterin in pigs.
abstract: The efficacy of currently available washed whole cell Streptococcus suis bacterins is generally poor. We developed and tested the efficacy of a novel ceftiofur-washed whole cell bacterin. Sixty-six, 2-week-old specific pathogen free (SPF) pigs were randomly divided into 5 groups. Three groups were vaccinated 28 and 14 d prior to challenge. The 3 ceftiofur-washed whole cell bacterins each contained 1 of 3 different adjuvants (Montanide ISA 25, Montanide ISA 50, and Saponin). Pigs exhibiting severe central nervous system disease or severe joint swelling and lameness were euthanized immediately and necropsied. All remaining pigs were necropsied at 14 d post inoculation. The ceftiofur-washed whole cell S. suis bacterin with Montanide ISA 50 adjuvant significantly (P < 0.05) reduced bacteremia, meningitis, pneumonia, and mortality associated with S. suis challenge. Further work on this novel approach to bacterin production is warranted.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pallarés
authors.forename: Cameron S
authors.initials: CS
authors.lastname: Schmitt
authors.forename: James A
authors.initials: JA
authors.lastname: Roth
authors.forename: Richard B
authors.initials: RB
authors.lastname: Evans
authors.forename: Joann M
authors.initials: JM
authors.lastname: Kinyon
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Halbur
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000517:prevention & control / Q000662:veterinary*; D001428:Bacterial Vaccines; D002511:Cephalosporins; D013047:Specific Pathogen-Free Organisms; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D017011:Streptococcus suis / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*
chemical_list: D001428:Bacterial Vaccines; D002511:Cephalosporins; C053503:ceftiofur