Definite peak identification of (R)-and (S)-methadone and (R)- and (S)-EDDP using established HPLC and CE methods.
Methadone is widely used for the treatment of opioid dependence. HPLC and CE are widespread methods for drug monitoring and metabolism studies. Although the methods are widely used for methadone and its main metabolite EDDP [1, 2], a definite direct peak identification for EDDP enantiomers is not described. This study describes a method for specific identification of each peak in the chromatogram and electropherogram of methadone analysis. The result of the study demonstrates differences in the elution order of the enantiomers of methadone and EDDP due to the technique used for analysis. The elution order of EDDP using HPLC is interchanged with respect to the order of the peaks in the electropherogram.
Trkulja
Sandra
S
Institute of Pharmacy, Department of Pharmaceutical Analysis, University of Tuebingen, Auf der Morgenstelle 8, D-72076 Tuebingen, Germany.
Kovar
Karl-Artur
KA
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D007202:Indicators and Reagents; D008691:Methadone / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011759:Pyrrolidines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
D007202:Indicators and Reagents; D011759:Pyrrolidines; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine