[Effect of hesperidin and rutin on oxidative modification of high density lipoprotein in vitro].
OBJECTIVE
To study the effect of hesperidin and rutin on oxidative modification of high density lipoprotein (HDL) in vitro.


METHODS
HDL was isolated from healthy human plasma by sequential ultracentrifugation, and was oxidized by copper ions. The inhibitory effects of hesperidin and rutin on HDL oxidative modification were valued by the formation of malondialdehyde (MDA).


RESULTS
Hesperidin and rutin significantly inhibited copper-induced oxidation of HDL in a dose-dependent manner.


CONCLUSIONS
Both hesperidin and rutin can prevent HDL from copper-induced oxidative modification in vitro. This result suggests that they might have antiatherogenic effect.
Li
Qin-Shan
QS
Deparment of Biochemistry, Zunyi Medical College, Zunyi Guizhou Province 563003, China. liqinshan333@yahoo.com.cn
Lou
Gui-Yu
GY
Qian
Min-Zhang
MZ
D001161:Arteriosclerosis / Q000188:drug therapy; D003300:Copper / Q000494:pharmacology; D006569:Hesperidin / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D012431:Rutin / Q000494:pharmacology*
D008075:Lipoproteins, HDL; D008315:Malondialdehyde; D012431:Rutin; D003300:Copper; D006569:Hesperidin