Cellular proliferation in the cerebral cortex following neural excitation in rats.
Cortical spreading depression (SD) is characterized by propagation of neuronal/glial membrane depolarization throughout the unilateral cerebral cortex and has been linked to several neurological disorders, including migraine aura and epilepsy. SD induction resulted in a dramatic increase in BrdU-incorporated cells in the ipsilateral cortical hemisphere that was dependent on the number of elicited SD. Immunohistochemical studies revealed that 53% of the BrdU-labeled cells in the SD-generated cortex were NG2 immunopositive and 25% were OX-42 immunopositive. The remaining 22% of BrdU-incorporated cells showed no immunoreactivity to GST-rr, GFAP, NeuN, NG2 or OX-42. These data indicate that functional excitation of the cerebral cortex induces proliferative response in cortical cells, which may subsequently differentiate into glial progenitor or microglia within 3 days after stimulation.
Tamura
Yasuhisa
Y
Department of Anatomy and Cell Science, Kansai Medical University, Moriguchi City, Osaka, Japan.
Kataoka
Yosky
Y
Cui
Yilong
Y
Yamada
Hisao
H
D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013181:Cortical Spreading Depression / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D008297:Male; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001973:Bromodeoxyuridine; D005904:Glial Fibrillary Acidic Protein; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4