Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing.
Human adenoviruses are common pathogens associated with many diseases, including respiratory, gastrointestinal, and ocular infections. Because they are now being increasingly recognized as agents of life-threatening disseminated infection in immunocompromised patients, robust and sensitive laboratory detection methods are needed for their rapid diagnosis. We describe here a PCR assay using a single primer pair, targeting a region of the hexon gene containing hypervariable region 7, that can detect all known human adenovirus serotypes and allows for serotype determination through the analysis of the nucleotide sequence. This comprehensive assay has proven effective for diagnosing adenoviruses at the serotype level in a broad range of patient specimens, including conjunctival, nasopharyngeal, stool, blood, and urine specimens.
Sarantis
Helen
H
Division of Microbiology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada.
Johnson
Grant
G
Brown
Martha
M
Petric
Martin
M
Tellier
Raymond
R
D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D017931:DNA Primers