title: Chemiluminescence detection of isoniazid using Ru(phen)3(2+)-isoniazid-Ce(IV) system.
abstract: In our experiment, it was observed that isoniazid could enhance the chemiluminescence (CL) emission of tris-(1,10-phenanthroline)ruthenium(II) (Ru(phen)3(2+))-cerium(IV) (Ce(IV)) system and this enhancement effect was dependent on the concentration of isoniazid, based on which, a novel CL system was established for the detection of isoniazid. Under the optimum experimental conditions, the dynamic range and detection limit are 7.0 x 10(-2) to 6.5 microg ml(-1) and 2.5 x 10(-2) microg ml(-1), respectively. The R.S.D. is 3.4% (n = 11). The proposed method has been applied to detect the content of isoniazid in the injection solution with satisfactory results. The possible mechanism of the CL reaction was studied.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xi
authors.forename: Bo'an
authors.initials: B
authors.lastname: Shi
authors.forename: Xinping
authors.initials: X
authors.lastname: Ai
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D002138:Calibration; D002563:Cerium / Q000737:chemistry*; D007538:Isoniazid / Q000032:analysis* / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013431:Sulfates / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; D013431:Sulfates; C034282:tris-(1,10-phenanthroline)ruthenium; C107379:cerium sulfate; D002563:Cerium; D007538:Isoniazid