title: The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion.
abstract: Epidermal growth factor (EGF) modulates Leydig cell proliferation, steroidogenesis, spermiogenesis, and Sertoli cell activity. It plays an important role in repairing ischemia-reperfusion injury in different tissues. The aim of this study was to evaluate the effects of sustained and local administration of EGF on improving bilateral testicular tissue after torsion. A total of 57 Wistar albino rats were used. For the EGF transport system, 1x2 cm gelatin films containing 2 microg EGF were used. Torsion was created by rotating the right testis 720 degrees in a clockwise direction for 4 h in all groups except the control group. Then, in the torsion group, bilateral orchiectomy was performed. After returning the torsioned ipsilateral testes to their normal state, the bilateral testes were wrapped by 1x2 cm unloaded gelatin films in the gelatin (G7 and G21) groups and, by 2 microg EGF loaded gelatin films in the EGF 7 and EGF 21 groups. The testes were removed on the seventh and 21st days, respectively, for biochemical and histological examination. Histologically, Johnsen's spermatogenesis criteria and mean seminiferous tubule diameter (MSTD) measurements were used. The EGF7 group did not show significant loss of Sertoli cells, while in the G7 group the number of these cells decreased. The ipsilateral ischemic testis of the EGF21 group showed Leydig cell hyperplasia, and the contralateral non-ischemic testes in this group were similar to the control group. In the G21 group, the bilateral testes showed Sertoli cell only syndrome in some sections, and most of the cells were undergoing apoptosis. The mean spermatogenesis scores and MSTD in the EGF7 and EGF21 groups were higher than in the G7 and G21 groups ( P<0.05). Malondialdehyde levels were significantly lower in the EGF groups than in the G groups ( P<0.05). Glutathione peroxidase (GSH-Px) levels in the G21 group were significantly higher than in the EGF21 group. Our study shows that local and sustained EGF release after testicular torsion improves bilateral testicular injury. EGF administration may be a new treatment choice for bilaterally injured testis after detorsion without removing the twisted testis.
authors.affiliation: Department of Pediatric Surgery, Inonu University, Turgut Ozal Medical Center, Malatya, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Uguralp
authors.forename: A
authors.initials: A
authors.lastname: Bay Karabulut
authors.forename: B
authors.initials: B
authors.lastname: Mizrak
authors.forename: F
authors.initials: F
authors.lastname: Kaymaz
authors.forename: Aysel
authors.initials: A
authors.lastname: Kiziltay
authors.forename: N
authors.initials: N
authors.lastname: Hasirci
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D006965:Hyperplasia; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012671:Seminiferous Tubules / Q000473:pathology; D012708:Sertoli Cells / Q000187:drug effects / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000473:pathology / Q000503:physiopathology*; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D004815:Epidermal Growth Factor; D005979:Glutathione Peroxidase