The cloning of cyclin B3 and its gene expression during hormonally induced spermatogenesis in the teleost, Anguilla japonica.
We cloned cyclin B1, B2, and B3 cDNAs from the eel testis. Northern blot analysis indicated that these cyclin B mRNAs were expressed and increased from day 3 onward after the hormonal induction of spermatogenesis, and that cyclin B3 was most dominantly expressed during spermatogenesis. In situ hybridization showed that cyclin B1 and B2 were present from the spermatogonium stage to the spermatocyte stage. On the other hand, cyclin B3 mRNA was present only in spermatogonia. Although mouse cyclin B3 is expressed specifically in the early meiotic prophase, these results indicate that eel cyclin B3 expression is limited during spermatogenesis to spermatogonia, but is not present in spermatocytes. These facts together suggest that eel cyclin B3 is specifically involved in spermatogonial proliferation (mitosis), but not in meiosis.
Kajiura-Kobayashi
Hiroko
H
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; CREST, Kawaguchi, Saitama 332-0012, Japan.
Kobayashi
Tohru
T
Nagahama
Yoshitaka
Y
D000811:Anguilla; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D003001:Cloning, Molecular; D019926:Cyclin B / Q000235:genetics*; D056744:Cyclin B1; D056765:Cyclin B2; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008540:Meiosis; D008938:Mitosis; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors; D014162:Transfection
C534699:CCNB1 protein, human; C503758:CCNB2 protein, human; C503694:CCNB3 protein, human; C534700:Ccnb1 protein, mouse; C503759:Ccnb2 protein, mouse; C503695:Ccnb3 protein, mouse; D019926:Cyclin B; D056744:Cyclin B1; D056765:Cyclin B2; D018076:DNA, Complementary; D012333:RNA, Messenger