title: A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene.
abstract: We developed a simple, quick assay in order to discriminate forensic samples among human, and common domestic and livestock species of the Pampean region, Argentina. A mitochondrial cytochrome b fragment amplified with universal primers was separately digested with three restriction enzymes (AluI, HaeIII, and HinfI) and the resulting fragments were resolved through electrophoresis in polyacrylamide gels. This PCR-RFLP method allowed us to identify the target species and worked on degraded samples. The assay was successfully applied in livestock robbery cases in Argentine, and may be useful when attempting a first assessment as to the specific status of a forensic evidence.
authors.affiliation: Facultad de Ciencias Veterinarias, Centro de Investigaciones en Genética Básica y Aplicada, Universidad Nacional de La Plata, 60 y 118 s/n, P.O. Box 296, B1900 AVW La Plata, Argentina.
authors.forename: Claudio M
authors.initials: CM
authors.lastname: Bravi
authors.forename: Juan P
authors.initials: JP
authors.lastname: Lirón
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Mirol
authors.forename: María V
authors.initials: MV
authors.lastname: Ripoli
authors.forename: Pilar
authors.initials: P
authors.lastname: Peral-García
authors.forename: Guillermo
authors.initials: G
authors.lastname: Giovambattista
mesh_terms: D000818:Animals; D001118:Argentina; D002415:Cats; D002417:Cattle; D002645:Chickens; D045303:Cytochromes b / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D018397:Equidae; D006736:Horses; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D045303:Cytochromes b; D004262:DNA Restriction Enzymes