Antibody zymography: a novel adaptation of zymography to determine the protease-neutralising potential of specific antibodies and snake antivenoms.
A common problem in the development of antibody-based therapeutics is the selection, usually from a large population, of specific antibodies with the desired function. One of our research objectives is to identify antibodies capable of neutralising the most important haemorrhagic and haemostasis-disruptive proteases from viper venom. Here, we describe a modification of conventional gelatin-zymography that permits the identification of antibodies capable of neutralising gelatinolytic proteases. We demonstrate that the gelatinolytic activity of viper venom proteases is neutralised by addition of viper antivenom to the matrix of conventional gelatin-zymograms. Venom protein gelatinolytic activity was unaffected by inclusion of antibody from control, non-immunised animals or immunoglobulin-depleted serum. The application of this antibody zymogram technique for future research on snake venoms is evaluated in the context of identified limitations.
Hasson
S S
SS
Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK.
Theakston
R D G
RD
Harrison
R A
RA
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000997:Antivenins / Q000276:immunology*; D010450:Endopeptidases / Q000276:immunology*; D005780:Gelatin / Q000378:metabolism*; D051379:Mice; D009500:Neutralization Tests; D014757:Viper Venoms / Q000276:immunology*
D000906:Antibodies; D000997:Antivenins; D014757:Viper Venoms; D005780:Gelatin; D010450:Endopeptidases