title: A Populus EST resource for plant functional genomics.
abstract: Trees present a life form of paramount importance for terrestrial ecosystems and human societies because of their ecological structure and physiological function and provision of energy and industrial materials. The genus Populus is the internationally accepted model for molecular tree biology. We have analyzed 102,019 Populus ESTs that clustered into 11,885 clusters and 12,759 singletons. We also provide >4,000 assembled full clone sequences to serve as a basis for the upcoming annotation of the Populus genome sequence. A public web-based EST database (POPULUSDB) provides digital expression profiles for 18 tissues that comprise the majority of differentiated organs. The coding content of Populus and Arabidopsis genomes shows very high similarity, indicating that differences between these annual and perennial angiosperm life forms result primarily from differences in gene regulation. The high similarity between Populus and Arabidopsis will allow studies of Populus to directly benefit from the detailed functional genomic information generated for Arabidopsis, enabling detailed insights into tree development and adaptation. These data will also valuable for functional genomic efforts in Arabidopsis.
authors.affiliation: Department of Biotechnology, Kungliga Tekniska Högskolan Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Sterky
authors.forename: Rupali R
authors.initials: RR
authors.lastname: Bhalerao
authors.forename: Per
authors.initials: P
authors.lastname: Unneberg
authors.forename: Bo
authors.initials: B
authors.lastname: Segerman
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.forename: Amy M
authors.initials: AM
authors.lastname: Brunner
authors.forename: Laurence
authors.initials: L
authors.lastname: Charbonnel-Campaa
authors.forename: Jenny Jonsson
authors.initials: JJ
authors.lastname: Lindvall
authors.forename: Karolina
authors.initials: K
authors.lastname: Tandre
authors.forename: Steven H
authors.initials: SH
authors.lastname: Strauss
authors.forename: Björn
authors.initials: B
authors.lastname: Sundberg
authors.forename: Petter
authors.initials: P
authors.lastname: Gustafsson
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.forename: Rishikesh P
authors.initials: RP
authors.lastname: Bhalerao
authors.forename: Ove
authors.initials: O
authors.lastname: Nilsson
authors.forename: Göran
authors.initials: G
authors.lastname: Sandberg
authors.forename: Jan
authors.initials: J
authors.lastname: Karlsson
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.forename: Stefan
authors.initials: S
authors.lastname: Jansson
mesh_terms: D017753:Ecosystem; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D006801:Humans; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D032107:Populus / Q000235:genetics*; D013045:Species Specificity
chemical_list: D010940:Plant Proteins