title: Measurement of C-reactive protein: two high sensitivity methods compared.
abstract: C-reactive protein (CRP) is an acute phase marker and a predictor of the risk of developing atherosclerotic complications. However, as a predictor of this risk, high sensitivity measurements are needed, and high sensitive CRP (hsCRP) assays have been developed. In this study, we experimentally compared two hsCRP assays, based on nephelometry and turbidimetry, both implemented on automated analyzers. Linearity, imprecision, turbidity interference, and results in the assay of 96 samples have been compared. Method comparison of the same two analytical systems in the assay of CRP was also performed on the basis of results in an interlaboratory external quality assessment scheme (EQAS). The two systems were found to perform substantially equally, both in hsCRP and in CRP measurement, but in the hsCRP assay the precision of nephelometry (CV% in the interval 3.0-5.8) was lower than that of turbidimetry (CV% in the interval 1.8-2.3). The classification of results by the two methods into three predefined relative risk classes gave 18% rate of discordance, in any case by one class only. The two methods proved reliable and comparable in the measurement of hsCRP, but precision should be improved.
authors.affiliation: Laboratorio Analisi Chimico-Cliniche, Ospedale Luigi Sacco, Milano, Italy. chimica.clinica@hsacco.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Dominici
authors.forename: Paola
authors.initials: P
authors.lastname: Luraschi
authors.forename: Carlo
authors.initials: C
authors.lastname: Franzini
mesh_terms: D001161:Arteriosclerosis / Q000097:blood / Q000175:diagnosis; D002097:C-Reactive Protein / Q000032:analysis*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D015510:Clinical Medicine / Q000379:methods*; D006801:Humans; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002097:C-Reactive Protein