title: The immunohistochemical analysis of pendrin in the mouse inner ear.
abstract: Pendred's syndrome (PS) is an autosomal recessive disorder characterized by deafness and goiter, which are caused by mutations in the Pendred's syndrome gene (PDS). PDS encodes a membrane protein named pendrin that is considered to act as an anion transporter. An expression pattern of the PDS ortholog (Pds) mRNA in the auditory and vestibular systems has been reported in mice, and the localization of pendrin has been reported recently. We generated antipeptide antibodies against human pendrin, and performed immunohistochemical analysis of mouse inner ears. We detected pendrin in the endolymphatic duct and sac, and the utricle, saccule, and external sulcus. In the endolymphatic duct and sac, the expression of pendrin was apparent at the apical membrane. In addition, we detected pendrin in the spiral ligament, Claudius cells, Deiter's cells, and the spiral ganglion of the cochlea. Our results are key to defining the role of pendrin in inner ear development and elucidating the pathogenic mechanisms underlying deafness in PS.
authors.affiliation: Department of Otorhinolaryngology, University of Nagoya, 65, Tsurumai-cho, Showa-ku, Nagoya 4668550, Japan. yoshinot@med.nagoya-u.ac.jp
authors.forename: Takahiko
authors.initials: T
authors.lastname: Yoshino
authors.forename: Eisuke
authors.initials: E
authors.lastname: Sato
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakashima
authors.forename: Wataru
authors.initials: W
authors.lastname: Nagashima
authors.forename: Masa-Aki
authors.initials: MA
authors.lastname: Teranishi
authors.forename: Atsuo
authors.initials: A
authors.lastname: Nakayama
authors.forename: Naoyoshi
authors.initials: N
authors.lastname: Mori
authors.forename: Hideki
authors.initials: H
authors.lastname: Murakami
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Funahashi
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Imai
mesh_terms: D000818:Animals; D027321:Anion Transport Proteins; D001921:Brain / Q000378:metabolism; D007758:Ear, Inner / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D000076988:Sulfate Transporters; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism
chemical_list: D027321:Anion Transport Proteins; D026901:Membrane Transport Proteins; C494849:Slc26a4 protein, mouse; D000076988:Sulfate Transporters