[Ciliogenesis in human nasal epithelial cells cultured at the air-liquid interface].
OBJECTIVE
In the present study, our aim was to develop a method to quantitative cell differentiation in cultures of human nasal epithelium (HNE) cells.


METHODS
HNE cells were cultured on collagengel-coated membranes at an air-liquid interface (ALI) in hormone-and growth factor-supplemented medium. The percentage of the culture surface covered with ciliated cells was estimated using scanning electron microscope and image analysis.


RESULTS
In present study, if an ALI was not established and the cells were maintained in the submerged state, ciliated cell differentiation was pool, the average ciliated surface area was 0.3% when cultures were submerged for fourteen days. When HNE cells cultures in ALI, average ciliated surface area was 8.6%.


CONCLUSIONS
Our study demonstrated increased ciliogenesis in ALI.
Guo
Yongqing
Y
Department of Otolaryngology, First Clinical Academy of Medical College of Xiamen University, Xiamen, 361004, China.
Zhao
Xiaodong
X
Yang
Zhanquan
Z
D002454:Cell Differentiation; D002478:Cells, Cultured; D002923:Cilia / Q000648:ultrastructure*; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009297:Nasal Mucosa / Q000166:cytology*
D003470:Culture Media