title: Fosfomycin and rifampin disk diffusion tests for detection of Escherichia coli mutator strains.
abstract: A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an overnight culture. Strains with <30 and <10 colonies in fosfomycin and rifampin inhibition zones are presumptively non-mutators.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. de Colmenar, Km 9,1, 28034 Madrid, Spain. jgalanm.hrc@salud.madrid.org
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Galán
authors.forename: Marta
authors.initials: M
authors.lastname: Tato
authors.forename: María-Rosario
authors.initials: MR
authors.lastname: Baquero
authors.forename: Carmen
authors.initials: C
authors.lastname: Turrientes
authors.forename: Fernando
authors.initials: F
authors.lastname: Baquero
authors.forename: José-Luis
authors.initials: JL
authors.lastname: Martinez
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005578:Fosfomycin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D005578:Fosfomycin; D012293:Rifampin