title: Iron-superoxide dismutase and monodehydroascorbate reductase transcripts accumulate in response to internode rubbing in tomato.
abstract: A cDNA encoding an iron-superoxide dismutase (Fe-SOD) was isolated by RACE-PCR from a Lycopersicon esculentum cDNA library. The Fe-SOD cDNA consists of a 746-bp open reading frame and is predicted to encode a protein of 249 amino acids with a calculated molecular mass of 27.9 kDa. The deduced amino acid sequence was very similar to other plant Fe-SODs and a potential chloroplastic targeting was found. To study the induction of oxidative burst in response to mechanical stimulation, the accumulation of Fe-SOD and monodehydroascorbate reductase (MDHAR) mRNAs was analysed in response to young growing internode rubbing in tomato plants. Northern analyses show that Fe-SOD mRNA and MDHAR mRNA accumulated in tomato internodes 10 min after the mechanical stimulation. These results suggest that reactive oxygen species are early involved in the response of a plant to a mechanical stimulation, such as rubbing. The nucleotide sequence data reported in this paper will appear in the NCBI Nucleotide Sequence Databases under the accession number AY262025.
authors.affiliation: Laboratoire de biologie et physiologie végétales, département de biologie, faculté des sciences de Tunis, campus universitaire, 1060 Tunis, Tunisia. interlisa@zWallet.com
authors.forename: Ichrak
authors.initials: I
authors.lastname: Ben Rejeb
authors.forename: Catherine
authors.initials: C
authors.lastname: Lenne
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leblanc
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Julien
authors.forename: Saïda
authors.initials: S
authors.lastname: Ammar
authors.forename: Sadok
authors.initials: S
authors.lastname: Bouzid
authors.forename: Abdelkader
authors.initials: A
authors.lastname: Ayadi
mesh_terms: D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D010812:Physical Stimulation; D035261:Plant Components, Aerial / Q000235:genetics / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C023927:monodehydroascorbate reductase (NADH)