Improved beta-lactam acylases and their use as industrial biocatalysts.
Whereas the beta-lactam acylases are traditionally used for the hydrolytic processing of penicillin G and cephalosporin C, new and mutated acylases can be used for the hydrolysis of alternative fermentation products as well as for the synthesis of semisynthetic beta-lactam antibiotics. Three-dimensional structural analyses and site-directed mutagenesis studies have increased the understanding of the catalytic mechanism of these enzymes. The yield of hydrolysis and synthesis has been greatly improved by process design, including immobilization of the enzyme and the use of alternative reaction media. Significant advances have also been made in the resolution of racemic mixtures by means of stereoselective acylation/hydrolysis using beta-lactam acylases.
Sio
Charles F
CF
Pharmaceutical Biology, University Centre for Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
Quax
Wim J
WJ
D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D002384:Catalysis; D002617:Chemical Industry / Q000295:instrumentation / Q000379:methods; D004345:Drug Industry / Q000379:methods* / Q000639:trends; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D010399:Penicillin Amidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods* / Q000639:trends; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D004800:Enzymes, Immobilized; D000581:Amidohydrolases; D010399:Penicillin Amidase; D001618:beta-Lactamases