Identification of two novel HLA class II alleles, DQB1*0311 and DQB1*0620.
Two novel HLA class II alleles have been identified in routine typing of a kidney transplant patient and a cord blood unit from the Australian Cord Blood Bank in Sydney. Sequence analysis of exon 2 of the DQB1 genes revealed the novel polymorphism. A substitution of A to C at nucleotide position 136 has been identified for the DQB1*0311 allele when compared to the closest-matched allele, DQB1*030201. An identical substitution has also been identified for the DQB1*0620 allele when compared to the closest-matched allele, DQB1*0602. The substitution results in an amino acid change from methionine to leucine at position 46 implicating different specificity and affinity of antigen binding.
Hogbin
J-P
JP
Molecular Genetics, Tissue Typing, Australian Red Cross Blood Service, Sydney, Australia.
Greville
W
W
Chapman
G
G
Kennedy
A
A
Velickovic
Z M
ZM
Dunckley
H
H
D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons / Q000235:genetics*; D014644:Genetic Variation; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational
D017931:DNA Primers; D006680:HLA Antigens