[Preventive effect of Breviscapus Injection on chronic hypoxic myocardium injury in rats].
OBJECTIVE
To study the preventive effect of Breviscapus Injection on the chronic hypoxic myocardium injury in rats and its mechanism.


METHODS
Transmission electron microscope and biochemical analysis were used to assay the therapeutic effect of Breviscapus Injection in rat model induced by 4 week's hypoxia.


RESULTS
The contents of malondialdehyde (MDA) and Ca(2+) in the homogenate of myocardial tissue of the hypoxic rats were significantly higher than those of the control rats. The activities of superoxide dismutase (SOD), nitric oxide synthase (NOS) in the homogenate of myocardial tissue and the plasmic NO levels of the hypoxic rats were significantly lower than those of the control rats. Breviscapus Injection reduced the contents of MDA and Ca(2+), and increased the levels of SOD, NOS and NO. Ultrastructural examination revealed that the injuries of the ultrastructure of heart in hypoxic rats were improved by treatment with Breviscapus Injection.


CONCLUSIONS
Breviscapus Injection can effectively prevent and treat the hypoxia-induced myocardial damage. One of its mechanisms may relate to its adjusting NO level, anti-damaging of free radicals and inhibiting calcium overload.
Mao
Sun-Zhong
SZ
Department of Biochemistry, Wenzhou Medical College, Wenzhou, Zhejiang Province 325027, China. lmao@wzmc.com
Yan
Zhe
Z
Xie
Yu-Peng
YP
D000818:Animals; D019659:Asteraceae; D002118:Calcium / Q000378:metabolism; D002908:Chronic Disease; D006321:Heart / Q000187:drug effects*; D000860:Hypoxia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007267:Injections; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D009569:Nitric Oxide / Q000097:blood; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D009569:Nitric Oxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D002118:Calcium