Potential screening assay for undetectable viruses on the basis of their capacity to induce alpha interferon production.
High interferon production by plasmacytoid dendritic cells (PDC) was unexpectedly noted after their coculture with CD4(+) cells from a healthy donor whose cells subsequently showed human herpesvirus type 6 and 7 infections. This release of interferon was not observed with uninfected normal CD4(+) cells. Induction of PDC interferon production could help screen for covert virus infections.
Schmidt
Barbara
B
Department of Medicine, Division of Hematology/Oncology, University of California School of Medicine, San Francisco, CA 94143-1270, USA.
Ashlock
Brittany
B
Neipel
Frank
F
Levy
Jay A
JA
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D015654:Herpesvirus 6, Human / Q000302:isolation & purification; D016199:Herpesvirus 7, Human / Q000302:isolation & purification; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D014777:Virus Diseases / Q000175:diagnosis* / Q000276:immunology; D014780:Viruses / Q000276:immunology* / Q000302:isolation & purification*
D016898:Interferon-alpha