4-Acetyl-12,13-epoxyl-9-trichothecene-3, 15-diol from Isaria japonica mediates apoptosis of rat bladder carcinoma NBT-II cells by decreasing anti-apoptotic Bcl-2 expression and increasing pro-apoptotic Bax expression.
We studied the effect of 4-acetyl-12,13-epoxyl-9-trichothecene-3, 15-diol (AETD) isolated from Isaria japonica, one of the most popular Chinese fungal medicines, on the induction of apoptosis in rat bladder carcinoma NBT-II cells. AETD was cytotoxic to NBT-II cells, and this cytotoxic effect appears to be attributed to its induction of apoptotic cell death, as AETD induced nuclear morphological changes and internucleosomal DNA fragmentation, and increased the proportion of hypodiploid cells and activity of caspase-3. AETD treatment also decreased the expression of the anti-apoptotic protein Bcl-2 and increased the expression of the pro-apoptotic protein Bax. These results provide important information in understanding the mechanism(s) of AETD-induced apoptosis.
Kim
Hyung-Jin
HJ
Immunopia Research Laboratory of Molecular and Cellular Immunology, Wonkwang University, Iksan, Chonbuk 570-749, South Korea.
Jang
Seon Il
SI
Kim
Young-Jun
YJ
Pae
Hyun-Ock
HO
Won
Hae-Young
HY
Hong
Kyung-Hwan
KH
Oh
Hyuncheol
H
Kwon
Tae-Oh
TO
Chung
Hun-Taeg
HT
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D008856:Microscopy, Fluorescence; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D014255:Trichothecenes / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D051028:bcl-2-Associated X Protein
C440202:4-acetyl-12,13-epoxy-9-trichothecene-3,15-diol; C494828:Bax protein, rat; D004273:DNA, Neoplasm; D019253:Proto-Oncogene Proteins c-bcl-2; D014255:Trichothecenes; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases