[Study on the expression of eIF families after elemene treatment].
OBJECTIVE
To investigate the activity of Caspase-3 and the expression of eukaryotic initiation factor families, bFGF and VEGF after elemene on laryngeal carcinoma HEp-2 cells.


METHODS
The HEp-2 cells after elemene treatment were analyzed utilizing Westernblot and reverse transcriptase polymerase chain reaction (RT-PCR). The activity of Caspase-3 was assessed by colorimetric assay.


RESULTS
The activity of Caspase-3 was enhanced after elemene treatment. The protein expression of eIF4E, eIF4G, bFGF and VEGF were significantly inhibited by elemene; and the mRNA expression of bFGF and VEGF were inhibited either.


CONCLUSIONS
Elemene can effectively inhibit the growth of HEp-2 cells and result in the alteration of activity of Caspase-3. There were significant correlations between the decreased expression of protein eIF4E, eIF4G, bFGF and VEGF. The mechanism of eIF4E and eIF4G decrease the expression of bFGF and VEGF is post-transcriptional.
Tao
Lei
L
Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University, Shanghai, 200031, China.
Zhou
Liang
L
D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis / Q000187:drug effects*; D045744:Cell Line, Tumor; D039561:Eukaryotic Initiation Factor-4E / Q000096:biosynthesis / Q000187:drug effects*; D039603:Eukaryotic Initiation Factor-4G / Q000096:biosynthesis / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012717:Sesquiterpenes / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000187:drug effects
D039561:Eukaryotic Initiation Factor-4E; D039603:Eukaryotic Initiation Factor-4G; D012717:Sesquiterpenes; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; C038905:elemene; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases