In vitro antispasmodic and anti-inflammatory effects of Drosera rotundifolia.
In investigations of the anti-inflammatory and spasmolytic effects of Drosera rotundifolia two extracts were tested in different in vitro assays. An aqueous and an ethanolic extract inhibited human neutrophil elastase, achieving IC50 values of 5 and 1 microg/mL, respectively. The very low naphthoquinone concentrations in the extracts seem not to be responsible for the effect, as the pure compounds were not effective in the test system used. Thus, flavonoids like hyperoside, quercetin and isoquercitrin, which were detected in the extracts in considerable concentrations, may contribute to the activity. These substances showed activity in the assay. Ellagic acid, detected especially in the ethanolic extract in higher amounts, was substantially less active than the flavonoids. In guinea-pig ileum the extracts led to an antispasmodic effect possibly by affecting an allosteric binding site of the muscarinic M3 receptors.
Krenn
Liselotte
L
Institut für Pharmacognosie, Universität Wien, Austria. liselotte.krenn@univie.ac.at
Beyer
Gabriele
G
Pertz
Heinz H
HH
Karall
Elisabeth
E
Kremser
Michaela
M
Galambosi
Bertalan
B
Melzig
Matthias F
MF
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D029787:Drosera / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010276:Parasympatholytics / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D043587:Receptor, Muscarinic M3 / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D010276:Parasympatholytics; D010936:Plant Extracts; D043587:Receptor, Muscarinic M3; D019272:Leukocyte Elastase