[Genetic identification of internal transcribed spacers sequence in rDNA of Artemisis iwayomogi Kitam. and other two Artemisia species].
OBJECTIVE
To make an useful identification method for the molecule of DNA on 3 herbs of Artemisia genus and compare the differences of the genes of Korean and Chinese species of Artemisia.


METHODS
Sequence of 3 herbs (Artemisia sacrorum Ledeb., Artemisia iwayomogi Kitam. and Artemisia capillaris Thunb.) was determined by PCR sequence system. DNA was extracted from rDNA/ITS (internal transcribed spacers) and 5.8 s. The analysis was based on the amplification through DNA sequence system.


RESULTS
There were profound differences between the Korean Artemisia and Artemisia sacrorum L. These 2 herbs had a difference in the PCR amplifications of the agarose gel electrophoresis. There was a slight difference in the analysis of the DNA sequence system, and the substitution percentage for ITS gene fragments sequence was 3.96%.


CONCLUSIONS
Analytic identification method on sequence system of ITS in rDNA is effective for these 3 herbs.
Kim
Sung-Yong
SY
Department of Identification of Chinese Drugs, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China. jcy115@han-mail.net
Chen
Jian-Wei
JW
Liu
Zhong-Quan
ZQ
Wang
Yong-Zhen
YZ
D019306:Artemisia / Q000235:genetics*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer