[Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues].
OBJECTIVE
To investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).


METHODS
One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.


RESULTS
EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.


CONCLUSIONS
Detection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.
Li
Feng
F
Department of Pathology, Shihezi University School of Medicine, Xinjiang 832002, China. patho-sh@mail.xj.cninfo.net
Chang
Bin
B
Li
Xin-xia
XX
Pang
Li-juan
LJ
Lu
Hong-fen
HF
Wang
Jian
J
Sun
Meng-hong
MH
Shi
Da-ren
DR
D000293:Adolescent; D000328:Adult; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000175:diagnosis / Q000378:metabolism*; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D016612:Paraffin Embedding; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000175:diagnosis / Q000378:metabolism; D012512:Sarcoma, Ewing / Q000175:diagnosis / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000175:diagnosis / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D014157:Transcription Factors