Isolation and molecular analysis of Umhda2 a gene encoding a histone deacetylase from Ustilago maydis.
By use of the polymerase chain reaction and synthetic oligonucleotides designed from conserved regions, we amplified a fragment of a gene from Ustilago maydis encoding a putative histone deacetylase. With this probe we isolated the full gene from a minigenomic library. The gene (designated as Umhda2) contains an open reading frame (ORF) of 1701bp encoding a protein of 566 amino acids. Multiple comparison analysis with other histone deacetylases suggests that the Umhda2 gene product belongs to the Rpd3-related family of proteins. The highest degree of homology with histone deacetylases from other organisms corresponded to Hdalp of Schizosaccharomyces pombe and Rpd3p of Saccharomyces cerevisiae with 64.2 and 62.2% of sequence similarity, respectively. It displayed a substantially lower similarity with another histone deacetylase from U. maydis (Hdalp, 52.4%). Semi-quantitative RTPCR results indicate that the gene is transcriptionally up-regulated during the in vitro yeast-to-mycelium dimorphic transition.
González-Prieto
Juan Manuel
JM
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del I.P.N, Unidad Irapuato, Apartado Postal 629, Irapuato Gto., Mexico.
Domínguez
Angel
A
Rosas-Quijano
Raymundo
R
Cervantes-Chávez
José Antonio
JA
Ruiz-Herrera
José
J
D000595:Amino Acid Sequence; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal; D015698:Genomic Library; D006655:Histone Deacetylases / Q000235:genetics*; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014588:Ustilago / Q000235:genetics*
D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D006655:Histone Deacetylases