title: Distribution and MK-801-induced expression of serine racemase mRNA in rat brain by real-time quantitative PCR.
abstract: We have used real-time quantitative PCR methods to evaluate the effect of the systemic administration of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine (MK-801; 0.4 mg/kg) on the gene expression of serine racemase in several brain areas of the rat. The levels of serine racemase mRNA in all the brain areas transiently increased after the administration. The present findings suggest that there is a link between the expression of serine racemase mRNA and the activity of the NMDA receptors.
authors.affiliation: Department of Pharmacology, School of Medicine, Tokai University, Isehara, Kanagawa 259-1143, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Oka
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*; D019998:Racemases and Epimerases / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D000099300:Serine Racemase
chemical_list: D016291:Dizocilpine Maleate; D018691:Excitatory Amino Acid Antagonists; D012333:RNA, Messenger; D019998:Racemases and Epimerases; D016194:Receptors, N-Methyl-D-Aspartate; D000099300:Serine Racemase