Cell culture media are potent antioxidants that interfere during LDL oxidation experiments.
In vitro cell-induced low-density lipoprotein (LDL) oxidation is a model frequently used for studies on antioxidant compounds which may be potentially antiatherogens. Using Cu2+ or the free radical generator 2,2'-azobis-[2-amidinopropane] dihydrochloride (AAPH) to oxidize human LDL, we showed that the cell culture media Ham's F10 and RPMI are potent antioxidants which reduce LDL-protective effect of various thyroid compounds. The culture media interfered with the compounds depending on their mechanism of action, and RPMI had the greatest antioxidant effect, completely hiding antioxidant efficiency of the compounds whatever the prooxidant agent was. We suggest some recommendations for study of antioxidant compounds using cell-induced LDL oxidation models.
Faure
Philippe
P
Unité de Biochimie, Pharmacologie et Toxicologie, UFR des Sciences Pharmaceutiques et Biologiques, 7, boulevard Jeanne d'Arc, BP 87900, 21079 Dijon, France.
Oziol
Lucie
L
Le Bihan
Marie-Laure
ML
Chomard
Philippe
P
D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D013963:Thyroid Hormones / Q000494:pharmacology
D000578:Amidines; D000975:Antioxidants; D003470:Culture Media; D005609:Free Radicals; D008077:Lipoproteins, LDL; D016877:Oxidants; D013963:Thyroid Hormones; C046728:2,2'-azobis(2-amidinopropane); D003300:Copper