title: Antibodies to triosephosphate isomerase in patients with neuropsychiatric lupus.
abstract: Sera from patients with neuropsychiatric lupus (NP lupus) were screened for antibodies to mouse choroid plexus cell line ECPC-4 by Western blotting. A 29-kDa protein band detected in NP lupus sera was identified as triosephosphate isomerase (TPI). Using Western blotting with TPI, TPI was confirmed as the reactive molecule in sera (6 of 14 samples) and in cerebrospinal fluids (1 of 2 samples) of patients with NP lupus. Enzyme-linked immunosorbent assay with TPI showed that the serum anti-TPI antibody index of 89.8 (SD, 70.1) in NP lupus group was significantly higher than in systemic lupus erythematosus without NP manifestations, 34.6 (29.6); scleroderma, 38.2 (39.9); polymyositis/dermatomyositis, 42.1 (51.5); and control, 31.7 (27.4) groups (p<0.02). Sensitivity, specificity, and positive and negative likelihood ratios of serum anti-TPI antibody index for NP lupus were 42.9%, 94.7%, 8.1%, and 0.6%, respectively. These results suggest that anti-TPI antibodies are closely associated with NP lupus.
authors.affiliation: Department of Internal Medicine II, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Japan. chiehiro@fmu.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Tomomi
authors.initials: T
authors.lastname: Seino
authors.forename: Yukio
authors.initials: Y
authors.lastname: Sato
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000134:cerebrospinal fluid; D001324:Autoantigens / Q000737:chemistry / Q000302:isolation & purification; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000201:enzymology* / Q000276:immunology*; D001523:Mental Disorders / Q000201:enzymology* / Q000209:etiology / Q000276:immunology*; D051379:Mice; D009422:Nervous System Diseases / Q000201:enzymology* / Q000209:etiology / Q000276:immunology*; D011817:Rabbits; D014305:Triose-Phosphate Isomerase / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D014305:Triose-Phosphate Isomerase