Clinical development and serological antibody responses in sheep and rabbits experimentally infested with Psoroptes ovis and Psoroptes cuniculi.
Psoroptes ovis of sheep origin, and Psoroptes cuniculi of rabbit origin were used in experimental infestations. In experiment I, groups of four rabbits and four sheep were infested with 50-100 mites of each isolate on the skin of the back (skin infestation, SI) or in the external auditory canal (aural infestation, AI). In rabbits, SI and AI with P. cuniculi and AI with P. ovis induced in all animals typical ear lesions and pronounced antibody reactions to P. cuniculi antigens in ELISA. After SI of rabbits with P. ovis no clinical signs were detected, no mites could be reisolated and no specific antibodies were detected. In sheep, P. ovis SI induced mange whereas AI did not induce typical clinical signs and mites could not be reisolated. In both these animal groups, ELISA revealed pronounced and comparable specific antibody reactions. After SI and AI with P. cuniculi no clinical symptoms were observed and no mites could be reisolated. Nevertheless, low levels of specific antibody were detected. In experiment II, clinical progression and antibody reactions to P. ovis SI in naive sheep were compared with sheep previously exposed to P. ovis or P. cuniculi. In both pre-exposed groups of animals, clinical signs appeared within 2 days after challenge infestation and three days earlier than in primarily infested sheep. Subsequently, no obvious difference in the clinical progression was observed between the three groups of animals. The results of this study document antigenetic crossreactivity of the two morphologically and genetically distinguishable Psoroptes species but differences in their biological behaviour and virulence which both are of epidemiological and taxonomic relevance.
Siegfried
E
E
Institute of Parasitology, VetSuisse Faculty, University of Zurich, Winterthurerstrasse 266a, CH-805 7 Zurich, Switzerland.
Ochs
H
H
Deplazes
P
P
D000818:Animals; D000906:Antibodies / Q000097:blood*; D000941:Antigens / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D008297:Male; D008924:Mite Infestations / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D040042:Psoroptidae / Q000276:immunology*; D011817:Rabbits; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms
D000906:Antibodies; D000941:Antigens