[Research of ginsenosides in kou zi qi using HPLC-MS-MS].
OBJECTIVE
To analyze the ginsenosides in Kou zi qi (rhizomes of Panax japonicus C. A. Mey. var. major (Burkill) C. Y. Wu et K. M. Feng), and to supply evidences for chemotaxanology of Panax species and clinical uses of Kou zi qi.


METHODS
The ginsenosides were isolated by HPLC, then the positive- and negative-ion API-MS/MS of constituents collected from HPLC were measured.


RESULTS
Eight ginsenosides were identified as ginsenoside Re, ginsenoside Ro, chikuseksusaponins IV, IVa, notoginsenoside R2, ginsenosides Rb1, Rc and Rd, respectively, based on comparison of retention time with those of standards by HPLC, and analysis on their API-MS/MS data. Ginsenoside Ro and chikuseksusaponin IVa are the major components of Kou zi qi.


CONCLUSIONS
This plant had a close relationship to P. stipuleanatus, P. zinginensis and P. japonicus var major; a relatively remote relationship to P. ginseng and P. quinquefolius, in a view of chemotaxanology. Ginsenoside Ro and chikuseksusaponin IVa might be the anti-inflammatory constituents of Kou zi qi.
Zou
Kun
K
Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. kzou@hotmail.com
Liu
Zhao-xia
ZX
Zhu
Shu
S
Cai
Shao-qing
SQ
Komatsu
Katsuko
K
D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*; D010802:Phylogeny; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D036145:Ginsenosides; C064378:ginsenoside Ro; C049864:ginsenoside Re