title: [A study of the abnormalities of human epiderm in keloids and hypertrophic scars].
abstract: OBJECTIVE
  To investigate the abnormalities of human epiderm in keloids and hypertrophic scars.
  
  
  METHODS
  Biopsies from ten untreated keloids (duration of disease 3 - 30 years) and ten hypertrophic scars (duration of disease 6 - 10 months) and five normal adult skin specimens. Total RNA was isolated from normal adult skin. A cDNA fragment (base 5941 - 6481 bp) of the full-length human Tenascin-C cDNA was synthesized by polymerase chain reaction and subcloned in pGEM-T-easy. Dioxigen-labeled anti-sense and sense probes were synthesized by using a Sp6/T7 in vitro RNA synthesis kit in the present of Dig-UTP. In situ hybridization was performed on 4% paraformaldehyde-fixed and wax-embedded sections of keloids and hypertrophic scars. NBT-NCIP was used in color detection. Immunohistochemical procedure. The sections were incubated with antibodies (anti-Tenascin-C, anti-CK-16 and anti-Ki-67). Ultrasensitive Streptavidin Peroxidase staining was performed following established procedures.
  
  
  RESULTS
  The study show that the proliferation of epidermal keratinocytes in keloids and hypertrophic scars is very clear. The expressions of Tenascin-C mRNA in keloids epidermal keratinocytes markedly increased in contrast with epidermal keratinocytes of hypertrophic scars and adult skin. The CK-16 and Ki-67 stainings significantly enhanced in the epidermal keratinocytes of keloids and hypertrophic scars.
  
  
  CONCLUSIONS
  The different expressions of Tenascin-C, CK-16 and Ki-67 among normal adult skin, keloids and hypertrophic scars show the abnormalities of epidermal keratinocytes proliferation and differentiation in keloids and hypertrophic scars.
authors.affiliation: Department of Burns, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: He
authors.forename: Chun-mao
authors.initials: CM
authors.lastname: Han
authors.forename: Jia-ping
authors.initials: JP
authors.lastname: Peng
mesh_terms: D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D004817:Epidermis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D019063:Tenascin / Q000235:genetics / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D019063:Tenascin; D007633:Keratins