Prevention of postischemic spinal cord injury by means of regional infusion of adenosine and L-carnitine dissolved in normothermic saline.
Spinal cord ischemia still remains an unsolved problem in modern aortic surgery. In this study, we investigated the effectiveness of combined agents such as adenosine and L-carnitine infused to the isolated segment of abdominal aorta in a rabbit model. Twenty-eight rabbits divided into four groups underwent 40 min of isolated infrarenal aortic occlusion. Group I animals received no medication. Group II received an infusion of 100 mg/kg L-carnitine in normothermic saline over the first 10 min of ischemia. Group III received 50 mg adenosine, and group IV received a combination of the two agents in the same fashion. Spinal cord function was evaluated at 24 and 72 hr after operation on the basis of Tarlov scale and similar results were obtained. After a second evaluation, spinal cords were harvested for histological examination. Group I animals were all paraplegics. Spinal cord function was partially intact in two of the group II animals with Tarlov scores of 5 in two and 4 in two whereas one of the rabbits could not hop with a score of 3, and the remaining two could not sit with scores of 1 and 0. The spinal cord function of group III animals was intact with Tarlov scores of 5 in three, 4 in two, and 3 and 1 in remaining ones. In the group IV animals, it was fully intact with Tarlov scores of 5. Histological examination in group I revealed marked enlargement of the vacuoles of glial cells in the white matter of spinal cord. Glial cells were deteriorated in some locations in group II whereas they were mostly protected in the third group. In group IV, histological examination revealed no evidence of spinal cord injury. In conclusion, combined infusion of adenosine and L-carnitine provided better protection against postischemic spinal cord injury than individual infusion of these agents.
Darçin
Osman Tansel
OT
Department of Cardiovascular Surgery, Harran University, Faculty of Medicine, Sanliurfa, Turkey. otdarcin@hotmail.com
Baktiroğlu
Lütfü
L
Ozkul
Yaşar
Y
Ozardali
Ilyas
I
Andaç
Mehmet Halit
MH
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000473:pathology; D001794:Blood Pressure / Q000502:physiology; D002331:Carnitine / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D007261:Infusions, Intra-Arterial; D008297:Male; D008955:Models, Cardiovascular; D009457:Neuroglia / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D010264:Paraplegia / Q000503:physiopathology / Q000517:prevention & control; D011817:Rabbits; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology; D012965:Sodium Chloride / Q000494:pharmacology*; D013119:Spinal Cord Injuries / Q000209:etiology* / Q000503:physiopathology / Q000517:prevention & control*; D020760:Spinal Cord Ischemia / Q000150:complications* / Q000188:drug therapy* / Q000503:physiopathology; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
D018696:Neuroprotective Agents; D014665:Vasodilator Agents; D012965:Sodium Chloride; D000241:Adenosine; D002331:Carnitine