title: [Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar].
abstract: OBJECTIVE
  To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.
  
  
  METHODS
  Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.
  
  
  RESULTS
  Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).
  
  
  CONCLUSIONS
  Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.
authors.affiliation: Department of Plastic and Aesthetic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Li
authors.forename: Jian-yi
authors.initials: JY
authors.lastname: Liu
authors.forename: Shu-xing
authors.initials: SX
authors.lastname: Ji
mesh_terms: D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D055513:Connective Tissue Growth Factor; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor; D003094:Collagen