Various immunization protocols for an acute motor axonal neuropathy rabbit model compared.
Various ganglioside immunization protocols were examined to refine the procedure for establishing an animal model of acute motor axonal neuropathy. The most effective was subcutaneous injection of an emulsion of 2.5mg of bovine brain ganglioside mixtures, keyhole lympet hemocyanin, and complete Freund's adjuvant to Japanese white rabbits, repeated at 3-week intervals. Under that protocol, all the rabbits developed marked flaccid paralysis associated with plasma anti-GM1 IgG antibody. This acute motor axonal neuropathy rabbit model also could be reproduced by the use of incomplete Freund's adjuvant, methylated bovine serum albumin, and New Zealand white rabbits. These results provide useful information for the confirmation of and further research on the model.
Susuki
Keiichiro
K
Department of Neurology, Dokkyo University School of Medicine, Kitakobayashi 880, Mibu, Shimotsuga, Tochigi 321-0293, Japan. ksusuki@dokkyomed.ac.jp
Nishimoto
Yukihiro
Y
Koga
Michiaki
M
Nagashima
Takahide
T
Mori
Izumi
I
Hirata
Koichi
K
Yuki
Nobuhiro
N
D000208:Acute Disease; D000818:Animals; D020274:Autoimmune Diseases of the Nervous System / Q000276:immunology / Q000517:prevention & control*; D001369:Axons / Q000276:immunology*; D001500:BCG Vaccine / Q000627:therapeutic use; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D005732:Gangliosides / Q000276:immunology; D016537:Gangliosidosis, GM1 / Q000276:immunology / Q000517:prevention & control; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization; D008297:Male; D009046:Motor Neurons / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D012710:Serum Albumin, Bovine / Q000276:immunology
D001500:BCG Vaccine; D005732:Gangliosides; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin