Novel sesquiterpenoid matrix metalloproteinase-3 inhibitors from an acid mine waste extremophile.
Berkeley Pit Lake is a 1500 ft deep abandoned open-pit copper mine filled with 30 billion gallons of acidic, metal-contaminated water. This harsh environment is proving to be a source of unusual, biologically active microorganisms. Bioassay-guided fractionation using signal transduction enzyme assays led to the isolation of three novel bisabolane sesquiterpenes and a novel coumarin. The isolation and characterization of these compounds are reported here.
Stierle
Andrea A
AA
Department of Chemistry, Montana Tech of The University of Montana, Butte, Montana 59701, USA. astierle@mtech.edu
Stierle
Donald B
DB
Kemp
Kourtney
K
D000107:Acetylation; D000143:Acids / Q000032:analysis*; D061945:Caspase Inhibitors; D003300:Copper; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061965:Matrix Metalloproteinase Inhibitors; D015394:Molecular Structure; D009009:Montana; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis*; D014867:Water / Q000032:analysis*
D000143:Acids; D061945:Caspase Inhibitors; D003374:Coumarins; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D012717:Sesquiterpenes; D014866:Waste Products; D014867:Water; D003300:Copper; C030123:coumarin