[Effects of the site and method of liposuction on differentiation of human preadipocytes].
OBJECTIVE
To illustrate the effects of the site and method of liposuction on differentiation of human preadipocytes.


METHODS
Forty-two fatty samples were obtained with liposuction, which were then divided into four groups according to operation sites (abdomen, hip or extremity) and the methods (conventional negative-pressure or syringe method). Each sample was treated with collagenase I to release preadipocytes for in vitro culture. Affected by the differentiation-induced agents for 9 days or 28 days, the cultured adipocytes were stained with Nile red and observed under a fluorescent microscope. The differentiation rates were examined with flow cytometric analysis and the quantity of intracytoplasmic lipids was determined with oil red O staining. The results were analyzed with independent samples t-test (Mann-Whitney) using SPSS 10.0.


RESULTS
There was no statistical difference in differentiation at the 9th day or 28th day among the preadipocytes obtained from the abdomen, hip or extremity with the negative-pressure method. The preadipocytes from the abdomen differentiated more at the 28th day than the 9th day (P < 0.05), which was not observed in the hip or the extremity. The preadipocytes obtained from the abdomen with the negative-pressure method differentiated more than those with the syringe method (P < 0.05).


CONCLUSIONS
No essential difference was found in preadipocyte differentiation among the liposuction sites, while the abdomen might have some superiority. The negative-pressure method of liposuction is the first option in future research of tissue engineering. The flow cytometric analysis is a convenient way to study preadipocyte differentiation.
Dong
Xiang-bing
XB
Department of Plastic Surgery, Peking University Third Hospital, Beijing 100083, China.
Li
Jian-ning
JN
Wang
Xia
X
Zhu
Li
L
Qin
Rong-sheng
RS
Xie
Hong-bin
HB
Li
Bi
B
Xia
You-chen
YC
D000005:Abdomen / Q000601:surgery; D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006615:Hip / Q000601:surgery; D006801:Humans; D015187:Lipectomy / Q000379:methods*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D010078:Oxazines
D010078:Oxazines; C044808:nile red