Hepatocellular ultrastructure after ischemia/reperfusion injury in human orthotopic liver transplantation.
The number of patients requiring organ transplants still outpaces the number of available transplantable organs. During the process of orthotopic liver transplantation (OLTx), donor organs undergo significant stress resulting from ischemia and reperfusion. Healthy organs respond to this stressful environment with compensatory mechanisms that ideally allow for complete recovery. However, "marginal" organs do not compensate as well. Hepatic steatosis typically renders an organ nontransplantable; a liver with 30% or more fat has a 25% chance of primary nonfunction (PNF) or graft failure after a technically sound operation. In this study, we report on the significant markers of cellular ultrastructural change in steatotic livers. These include glycogen content, mitochondrial swelling, and hepatocellular blebbing. The data disclosed here argue that further investigation of these factors in marginal organs subjected to I/R may better facilitate our understanding of PNF.
Nadig
Satish N
SN
Department of Surgery, Division of Transplantation, Medical University of South Carolina, Charleston, South Carolina 29425, USA.
Periyasamy
Basker
B
Shafizadeh
Stephen F
SF
Polito
Carmen
C
Fiorini
Ryan N
RN
Rodwell
David
D
Evans
Zachary
Z
Cheng
Gang
G
Dunkelberger
Dana
D
Schmidt
Michael
M
Self
Sally E
SE
Chavin
Kenneth D
KD
D000704:Analysis of Variance; D016009:Chi-Square Distribution; D005234:Fatty Liver / Q000473:pathology; D005629:Frozen Sections; D006801:Humans; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D016031:Liver Transplantation; D008854:Microscopy, Electron; D011446:Prospective Studies; D015427:Reperfusion Injury / Q000503:physiopathology*