Large-scale gene expression analysis of human skeletal myoblast differentiation.
To study pathways involved in human skeletal myogenesis, we profiled gene expression in human primary myoblast cells derived from three individuals using both oligonucleotide and cDNA microarrays. Following stringent statistical testing (false-positive rate 0.4%), we identified 146 genes differentially expressed over time. Interestingly, 86 of these genes have not been reported to be involved in myogenesis in mouse cell lines. This demonstrates the additional value of human primary cell cultures in the study of muscle differentiation. Many of the identified genes play a role in muscle regeneration, indicating the close relationship of this process with muscle development. In addition, we found overlap with expression profiling studies in muscle from Duchenne muscular dystrophy patients, confirming ongoing muscle regeneration in Duchenne muscular dystrophy. Further study of these genes can bring new insights into the process of muscle differentiation, and they are candidate genes for neuromuscular disorders with an as yet unidentified cause.
Sterrenburg
Ellen
E
Center for Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
Turk
Rolf
R
't Hoen
Peter A C
PA
van Deutekom
Judith C T
JC
Boer
Judith M
JM
van Ommen
Gert-Jan B
GJ
den Dunnen
Johan T
JT
D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D016000:Cluster Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007211:Indoles / Q000378:metabolism; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000378:metabolism*; D051787:Myogenic Regulatory Factor 5; D009218:Myosins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D003893:Desmin; D007211:Indoles; C501369:LAMA4 protein, human; C501370:Lama4 protein, mouse; D007797:Laminin; C497417:MYF5 protein, human; D008840:Microfilament Proteins; D009124:Muscle Proteins; D051787:Myogenic Regulatory Factor 5; D012333:RNA, Messenger; D015534:Trans-Activators; C051615:transgelin; C007293:DAPI; D009218:Myosins