A 14-3-3 protein of Chlamydomonas reinhardtii associated with the endoplasmic reticulum: nucleotide sequence of the cDNA and the corresponding gene and derived amino acid sequence.
Two major 14-3-3 proteins of the unicellular green alga Chlamydomonas reinhardtii were purified and partially sequenced. The obtained data show that the 30-kDa isoform predominant in the cytosol is encoded by a previously cloned and sequenced 14-3-3 cDNA whereas the 27-kDa isoform represents a new 14-3-3 protein which is largely associated with the endoplasmic reticulum (ER). Therefore, the corresponding cDNA was cloned and sequenced. The nucleotide sequence of this new cDNA species and the derived amino acid sequence differ considerably from the previously cloned Chlamydomonas 14-3-3 cDNA. The conclusion that the divergent evolution of the corresponding genes must have started rather early as compared to the 14-3-3 genes of other organisms was corroborated by their different genomic organization. The amino acid sequences of both 14-3-3 isoforms were comparatively analysed to find differences which might be responsible for their differential binding to the ER.
Voigt
Jürgen
J
Physiologisch-chemisches Institut, Eberhard-Karls-Universität Tübingen, Germany. juergen.voigt@medizin.uni-leipzig.de
Stevanovic
Stefan
S
Schirle
Markus
M
Fausel
Martina
M
Maier
Josef
J
Adam
Karl-Heinz
KH
Marquardt
Otfried
O
D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002413:Cations, Divalent; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000378:metabolism; D014357:Trypsin
D048948:14-3-3 Proteins; D002413:Cations, Divalent; D018076:DNA, Complementary; D010446:Peptide Fragments; D020033:Protein Isoforms; D014357:Trypsin