title: Widespread occurrence of a novel division of bacteria identified by 16S rRNA gene sequences originally found in deep marine sediments.
abstract: Phylogenetic analysis of 16S rRNA gene sequences from deep marine sediments identified a deeply branching clade, designated candidate division JS1. Primers for PCR amplification of partial 16S rRNA genes that target the JS1 division were developed and used to detect JS1 sequences in DNA extracted from various sedimentary environments, including, for the first time, coastal marine and brackish sediments.
authors.affiliation: Cardiff School of Biosciences, University of Cardiff, Main Building, Park Pl., P.O. Box 915, Cardiff CF10 3TL, Wales, United Kingdom.
authors.forename: Gordon
authors.initials: G
authors.lastname: Webster
authors.forename: R John
authors.initials: RJ
authors.lastname: Parkes
authors.forename: John C
authors.initials: JC
authors.lastname: Fry
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Weightman
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012623:Seawater / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S