[Germline E-cadherin gene mutation screening in familial gastric cancer kindreds].
OBJECTIVE
To evaluate the frequency and nature of E-cadherin gene (CDH1) germline mutations in familial gastric cancer kindreds of china.


METHODS
Five familial gastric cancer kindreds of Chinese origin were screened for germline CDH1 mutations, all of them meeting the clinical criteria for hereditary diffuse gastric cancer (HDGC), by PCR-DHPLC and direct sequencing.


RESULTS
A new truncating mutation in exon 10 in B family was identified, producing a stop codon at position 503 (Q503X), resulting in a truncated protein. The proband of this family had metachronous development of lobular breast and diffuse type gastric carcinoma. No protein expression was detected in the lobular breast carcinoma, indicating complete inactivation of the gene.


CONCLUSIONS
A Chinese gastric cancer family with CDH1 germline truncating mutation is described for the first time, and our findings suggest that lobular breast carcinoma might be part of the tumor spectrum of HDGC.
Jiang
Yong
Y
Department of General Surgery, First Hospital, Peking University, Beijing 100034, China.
Wan
Yuan-Lian
YL
Wang
Zhen-Jun
ZJ
Zhao
Bo
B
Zhu
Jing
J
Huang
Yan-Ting
YT
D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009378:Neoplasms, Multiple Primary / Q000235:genetics; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*
D015820:Cadherins