title: Amplification of inhibitory activity of catechin against disease-related enzymes by conjugation on poly(epsilon-lysine).
abstract: A new inhibitor against disease-related enzymes, collagenase, hyaluronidase, and xanthine oxidase, has been developed by the laccase-catalyzed conjugation of catechin on poly(epsilon-lysine). The resulting poly(epsilon-lysine)-catechin conjugate showed greatly improved inhibition effects on activity of these enzymes, whereas the catechin monomer showed very low inhibition activity. The kinetic analysis on the inhibition of collagenase exhibited that the conjugate was a mixed-type inhibitor. The amplified activities might offer high potential as a therapeutic agent for prevention of various enzyme-related diseases.
authors.affiliation: Department of Materials Chemistry, Graduate School of Engineering, Kyoto University, 615-8510 Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ihara
authors.forename: Sarah
authors.initials: S
authors.lastname: Schmitz
authors.forename: Motoichi
authors.initials: M
authors.lastname: Kurisawa
authors.forename: Joo Eun
authors.initials: JE
authors.lastname: Chung
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uyama
authors.forename: Shiro
authors.initials: S
authors.lastname: Kobayashi
mesh_terms: D002392:Catechin / Q000737:chemistry* / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D011107:Polylysine; D002392:Catechin