Electrochemiluminescence detection with integrated indium tin oxide electrode on electrophoretic microchip for direct bioanalysis of lincomycin in the urine.
In this article, an antibiotic, lincomycin was determined in the urine sample by microchip capillary electrophoresis (CE) with integrated indium tin oxide (ITO) working electrode based on electrochemiluminescence (ECL) detection. This microchip CE-ECL system can be used for the rapid analysis of lincomycin within 40s. Under the optimized conditions, the linear range was obtained from 5 to 100 microM with correlation coefficient of 0.998. The limit of detection (LOD) of 3.1 microM was obtained for lincomycin in the standard solution. We also applied this method to analyzing lincomycin in the urine matrix. The limit of detection of 9.0 microM was obtained. This method can determine lincomycin in the urine sample without pretreatment, which demonstrated that it is a promising method of detection of lincomycin in clinical and pharmaceutical area.
Zhao
Xiaocui
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China.
You
Tianyan
T
Qiu
Haibo
H
Yan
Jilin
J
Yang
Xiurong
X
Wang
Erkang
E
D000900:Anti-Bacterial Agents / Q000652:urine*; D002021:Buffers; D004129:Dimethylpolysiloxanes; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007204:Indium; D008034:Lincomycin / Q000652:urine*; D008163:Luminescent Measurements; D008838:Microcomputers; D015203:Reproducibility of Results; D012828:Silicones; D017971:Tin Compounds
D000900:Anti-Bacterial Agents; D002021:Buffers; D004129:Dimethylpolysiloxanes; D007202:Indicators and Reagents; D012828:Silicones; D017971:Tin Compounds; D007204:Indium; C013830:baysilon; D008034:Lincomycin; C045358:stannic oxide