title: Alteration of the timing of implantation by in vivo gene transfer: delay of implantation by suppression of nuclear factor kappaB activity and partial rescue by leukemia inhibitory factor.
abstract: Nuclear factor kappaB (NF-kappaB) is activated in the murine endometrium during implantation period [Am. J. Reprod. Immunol. 51 (2004) 16]. Transient transfection of IkappaBalpha mutant (IkappaBalphaM) cDNA into the mouse uterine cavity using hemagglutinating virus of Japan envelope vector suppressed uterine NF-kappaB activity less than half of that observed in control on days 3.5 and 4.5 p.c. IkappaBalphaM cDNA transfection led to significant delay of implantation. After IkappaBalphaM cDNA transfection, LIF mRNA expression in the uterus was significantly suppressed on days 3.5 and 4.5 p.c. Co-transfection of LIF cDNA with IkappaBalphaM cDNA in the uterus partially rescued the delay of implantation induced by suppression of NF-kappaB activity. Taken together, these findings indicate that NF-kappaB activation determines the timing of the implantation, at least in part, via control of LIF expression.
authors.affiliation: Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, 2-2, Yamadaoka, Suita, Osaka 5650871, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Ogita
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Koyama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsujie
authors.forename: Tateki
authors.initials: T
authors.lastname: Tsutsui
authors.forename: Koichiro
authors.initials: K
authors.lastname: Shimoya
authors.forename: Masayasu
authors.initials: M
authors.lastname: Koyama
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kaneda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000818:Animals; D001483:Base Sequence; D051546:Cyclooxygenase 2; D018076:DNA, Complementary / Q000235:genetics; D010064:Embryo Implantation / Q000235:genetics* / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D053605:Leukemia Inhibitory Factor; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009154:Mutation; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014599:Uterus / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D020672:I-kappa B Proteins; D015850:Interleukin-6; D007527:Isoenzymes; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; C092119:Hoxa10 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases