Exposure to genistein does not adversely affect the reproductive system in adult male mice adapted to a soy-based commercial diet.
Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may affect adversely the development of male reproductive system. Five-week-old ICR mice were purchased and fed with a soybean-based Purina Chow diet until 6 months of age. The animals were exposed by gavage to genistein (2.5 mg/kg/day) or 17beta-estradiol (7.5 microg/kg/day) for five weeks. Corn oil was used for the negative control. The animals were fed the casein-based AIN-76A diet throughout the experimental periods. There were no significant differences in body and organ weights of mice among experimental groups. No significant differences in sperm counts and sperm motile characteristics were found between the control and the genistein groups. Treatment of 17beta-estradiol caused a significant decrease in epididymal sperm counts compared to the control (p<0.05). The level of phospholipid hydroxide glutathione peroxidase in the epididymis of mice exposed to genistein was significantly higher than that of the control mice (p<0.05). 17beta-estradiol treatment caused a reduction of germ cells in the testis and hyperplasia of mucosal fold region in the prostate of mice. Genistein treatment did not cause any lesion in the testis, epididymis, and prostate. These results suggest that dietary uptake of genistein at adult stage of life may not affect male reproductive system and functions.
Lee
Beom Jun
BJ
Department of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
Kang
Jong Koo
JK
Jung
Eun Yong
EY
Yun
Young Won
YW
Baek
In Jeoung
IJ
Yon
Jung Min
JM
Lee
Yoon Bok
YB
Sohn
Heon Soo
HS
Lee
Jae Yong
JY
Kim
Kang Sung
KS
Nam
Sang Yoon
SY
D000818:Animals; D004958:Estradiol / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D005837:Genitalia, Male / Q000187:drug effects* / Q000473:pathology; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D011467:Prostate / Q000187:drug effects / Q000473:pathology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013025:Glycine max; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology
D004968:Estrogens, Non-Steroidal; D004958:Estradiol; D012313:RNA; D019833:Genistein; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase