title: Natural antisense transcripts of HIF-1alpha are conserved in rodents.
abstract: A natural antisense transcript (aHIF), which sequence is strictly complementary to the 3' untranslated region (3'UTR) of HIF-1alpha mRNA, has been identified in human and shown to be overexpressed in renal carcinomas. We searched for aHIF in different rodent tissues. Two candidate expressed sequence tag (EST) were identified in silico and their PCR products (1.1 and 1.0 kb) were cloned and sequenced in mouse and rat, respectively. These transcripts were rigorously complementary to the 3'UTR of rodent HIF-1alpha mRNA and were broadly expressed in all mouse and rat tissues we tested. The conservation of aHIF in rodents underlined its potential importance in cell regulations. Therefore the responses of aHIF and HIF-1alpha transcripts were investigated in various types of hypoxic conditions. In freshly isolated rat renal tubules, aHIF RNA level was increased by acute hypoxia and low in normal supply of oxygen. In a rat strain raised in chronic hypobaric altitude hypoxia, aHIF transcript was greatly induced in the oxidative-type soleus and heart muscles of 3 month-old animals. By contrast, in the glycolytic-type extensor digitorum longus muscle aHIF transcript amount was lowered by hypoxia whereas HIF-1alpha transcript was highly expressed. In brain, where oxidative glycolysis takes place, HIF-1alpha mRNA and its antisense transcript levels were high and not significantly changed by altitude. Tumour cell lines cultured for 6 h in conditions mimicking hypoxia expressed lower amounts of HIF-1alpha mRNA. In two rat cell lines, aHIF transcript levels were greatly augmented after a 6-h incubation in these conditions, whereas in a mouse cell line, aHIF level was significantly reduced.
authors.affiliation: Laboratoire Inter-Universitaire des Activités Physiques et Sportives, Faculté de Médecine, 28 Place Henri Dunant, 63001 Clermont-Ferrand Cedex, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Rossignol
authors.forename: Elodie
authors.initials: E
authors.lastname: de Laplanche
authors.forename: Rémi
authors.initials: R
authors.lastname: Mounier
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bonnefont
authors.forename: Anne
authors.initials: A
authors.lastname: Cayre
authors.forename: Catherine
authors.initials: C
authors.lastname: Godinot
authors.forename: Hélène
authors.initials: H
authors.lastname: Simonnet
authors.forename: Eric
authors.initials: E
authors.lastname: Clottes
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D020413:3' Untranslated Regions; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016372:RNA, Antisense; D012333:RNA, Messenger; D014157:Transcription Factors; D010100:Oxygen