Tissue distribution and nucleotide sequence of bovine mRNA for salivary proline-rich protein P-B.
The tissue distribution of P-B was investigated to obtain information on the physiological significance of this proline-rich protein. To design primers and probes for a tissue distribution analysis, a polymerase chain reaction (PCR)-based cloning of bovine P-B cDNA was performed using tooth germ and the nucleotide sequence was determined. The cloned bovine P-B cDNA was composed of 356 bp and included the region corresponding to the mature P-B protein and part of the 3' non-coding sequence. This part of the sequence is identical to the corresponding region of human P-B cDNA from the submaxillary gland. DNA corresponding to the P-B mRNA was amplified by PCR using cDNAs from various bovine tissues including tooth germ, submaxillary gland, parotid gland, lachrymal gland, heart, liver, stomach, pancreas, spleen, kidney, adrenal, and ovary. A quantitative analysis indicated the heart, submaxillary gland, tooth germ and kidney to be major sites of P-B expression. The ubiquitous distribution of P-B mRNA among bovine tissues together with findings of the presence of genes hybridizable with a DNA probe for P-B among species such as human, bovine, rat, mouse, and yeast as reported previously suggested a fundamental physiological role for this protein.
Isemura
Satoko
S
Nippon Dental University Junior College, Niigata 951-8580, Japan. isemura@ngt.ndu.ac.jp
Watanabe
Sachiyo
S
Fujiwara
Shigeki
S
Sanada
Kazuo
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D055232:Proline-Rich Protein Domains; D012333:RNA, Messenger / Q000235:genetics; D012463:Saliva / Q000378:metabolism*; D014018:Tissue Distribution; D014083:Tooth Germ / Q000378:metabolism
D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger