Palmitoylputrescine, an antibiotic isolated from the heterologous expression of DNA extracted from bromeliad tank water.
Heterologous expression of large fragments of microbial DNA extracted directly from environmental samples (environmental DNA, or eDNA) in easily cultured hosts should provide access to some of the natural products produced by previously uncultured bacteria. The natural product antibiotic palmitoylputrescine (1) was isolated from Escherichia coli transformed with a cosmid (pCSLF16) containing DNA extracted directly from Costa Rican bromeliad tank water. In this report we describe the characterization of this antibiotic and its biosynthetic gene.
Brady
Sean F
SF
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
Clardy
Jon
J
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011700:Putrescine / Q000031:analogs & derivatives / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D014867:Water
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C492309:palmitoylputrescine; D014867:Water; D011700:Putrescine