title: Study of flow rate in pressurized gradient capillary electrochromatography using splitter and separation of peptides using an Amide stationary phase.
abstract: A pressurized gradient capillary electrochromatograph using a splitter was constructed. The variation in flow rate during gradient elution was investigated and separations of peptides using an Amide stationary phase were demonstrated. The flow rate, which is one of the important factors to control chromatographic behavior, was increased during the gradient elution, and the mismatching of mobile phase between the column and the resistance tubing derived three variation patterns in the flow rate. The electrophoretic migration in electrochromatography could enhance in separation of peptides. The separated peak number of tryptic digest of bovine serum albumin was increased from 30 to 40 by the application of +5 kV.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Nakashima
authors.forename: Shinya
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Tatsunari
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuda
mesh_terms: D000577:Amides / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D010455:Peptides / Q000302:isolation & purification*; D011312:Pressure
chemical_list: D000577:Amides; D010455:Peptides