Localization and regulation of glucagon receptors in the chick eye and preproglucagon and glucagon receptor expression in the mouse eye.
Myopia is a condition in which the eye is too long for the focal length of cornea and lens. Analysis of the messengers that are released by the retina to control axial eye growth in the animal model of the chicken revealed that glucagon-immunoreactive amacrine cells are involved in the retinal image processing that controls the growth of the sclera. It was found that the amount of retinal glucagon mRNA increased during treatment with positive lenses and pharmacological studies supported the idea that glucagon may act as a stop signal for eye growth. Glucagon exerts its regulatory effects by binding to a single type of glucagon receptor. In this study, we have sequenced the chicken glucagon receptor and compared its DNA and amino acid sequence with the human and mouse homologues. After sequencing about 80% of the receptor, we found a homology between 79.4 and 75.6% on cDNA level. At the protein level, about 73% of the amino acids were identical. Moreover, the cellular localization and regulation of the glucagon receptor in the chick retina was studied. In situ hybridization studies showed that many cells in the ganglion cell layer and inner nuclear layer, and some cells in the outer nuclear layer, express the receptor mRNA. Injection of the glucagon agonist Lys17,18,Glu21-glucagon induced a down-regulation of glucagon receptor mRNA content. Since the mouse would be an attractive mammalian model to study the biochemical and genetic basis of myopia, and because recent studies have demonstrated that form deprivation myopia can be induced, the expression of preproglucagon and glucagon receptor genes were also studied in the mouse retina and were found to be expressed.
Feldkaemper
Marita P
MP
Section of Neurobiology of the Eye, University Eye Hospital Tuebingen, Calwerstrasse 7/1, 72076, Germany. marita.feldkaemper@uni-tuebingen.de
Burkhardt
Eva
E
Schaeffel
Frank
F
D025042:Amacrine Cells / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D015536:Down-Regulation; D005123:Eye / Q000254:growth & development*; D005786:Gene Expression Regulation / Q000235:genetics; D005934:Glucagon / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D051379:Mice; D052336:Proglucagon; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018027:Receptors, Glucagon / Q000235:genetics*; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D011498:Protein Precursors; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D052336:Proglucagon; D004247:DNA; D005934:Glucagon