title: [Establishment of detection method for HCMVpp65 of the blood donors and its application in blood bank].
abstract: To establish method suitable to assay HCMVpp65 of the blood donors in blood bank and to supply safe blood to the patients, the immunocytochemical techniques were used, (6 - 8) x 10(6)/ml cells were counted, 50 x 10(3) cells were detected by light microscope, The results showed that 10 positive samples in 103 samples were found, positive rate was 9.71%, among 10 positive samples, 2 samples were still positive in the second detecting. In conclusion, this method is simple, quick and effective, suitable to detect HCMVpp65 of the blood donors in the blood bank.
authors.affiliation: Jiangsu Province Blood Center, Nanjing 210042, China.
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Liu
authors.forename: Hon-Li
authors.initials: HL
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Rong-Cai
authors.initials: RC
authors.lastname: Tang
authors.forename: Da-Xiang
authors.initials: DX
authors.lastname: Sheng
mesh_terms: D001771:Blood Banks; D001782:Blood Donors; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010750:Phosphoproteins / Q000097:blood*; D014763:Viral Matrix Proteins / Q000097:blood*
chemical_list: D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C464963:pp67 protein, human cytomegalovirus