Estimation of the cellular antioxidant response to chromium action using ESR method.
In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.
Kartvelishvili
Tamar
T
Institute of Molecular Biology and Biophysics, Georgian Academy of Sciences, 14, Gotua Str., 0160 Tbilisi, Georgia. tkartvelishvili@iphac.ge
Abuladze
Marina
M
Asatiani
Nino
N
Akhvlediani
Joseph
J
Kiziria
Eugene
E
Asanishvili
Lali
L
Lejava
Lia
L
Holman
Hoi-Ying N
HY
Sapojnikova
Nelly
N
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002457:Cell Extracts; D002460:Cell Line; D002857:Chromium / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D019810:Sodium Azide / Q000494:pharmacology
D000975:Antioxidants; D002457:Cell Extracts; D002857:Chromium; D019810:Sodium Azide; D002374:Catalase; D005978:Glutathione