Apolipoprotein E4 allele and ribosomal genes in Alzheimer's disease.
Ribosomal genes are involved in cellular transcription, translation and gene expression modulation process. An association between 28S/18S rRNA ratio levels with apoptosis and aging has been reported. Moulder et al. [22] and Hashimoto et al. [8] showed an association between apolipoprotein E4 allele and neuronal cell apoptosis through diverse mechanisms. The apoE 4 allele is considered a late-onset Alzheimer's disease (AD) risk factor associated with AD pathogenesis. We evaluated the association between apoE4 allele genotyping by PCR and rRNA 28S/18S ratio by slot blotting technique using peripheral blood samples of 18 Alzheimer's disease patients, 18 elderly controls and 18 young controls. A rRNA ratio decrease was observed in AD individuals confirming our previous results but this association is independently of the ApoE4 allele genotype. Thus our results pointed that two different mechanisms are involved in the etiology of Alzheimer disease each one leading independently to cell death. Further studies could investigate these factors.
Tavares
Wagner Malagó
WM
Disciplina de Biologia Molecular, Hemocentro FAMEMA, Faculdade de Medicina de Marília, São Paulo, Brasil.
Sperança
Marcia Aparecida
MA
de Labio
Roger Willian
RW
Peres
Clovis A
CA
Okamoto
Ivan Hideyo
IH
Bertolucci
Paulo Henrique Ferreira
PH
de A C Smith
Marília
M
Payão
Spencer Luiz Marques
SL
D000368:Aged; D000375:Aging / Q000502:physiology; D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction
D001057:Apolipoproteins E; D004275:DNA, Ribosomal