title: Depletion of Schistosoma mansoni lung-stage schistosomula cholesterol by methyl-beta-cyclodextrin dramatically increases specific antibody binding to surface membrane antigens.
abstract: Schistosoma mansoni lung-stage larvae appear to not bind antibodies from radiation vaccine or infection sera in the membrane immunofluorescence test. However, treatment of ex vivo lung-stage schistosomula with methyl-beta-cyclodextrin, a hydrophobic oligosaccharide that specifically extracts cholesterol from plasma membranes, induced readily detectable binding of specific antibodies in a concentration- and time-dependent manner. Surface membrane antigen binding of specific antibodies was also conclusively demonstrated by quantitative absorption of anti-schistosome sera with intact ex vivo larvae. These data together suggest that confinement of lung-stage schistosomula surface membrane antigens in cholesterol-rich sites allows only monovalent antibody binding, which can be detected by absorption and not by direct serology.
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt. rashika@mailer.scu.eun.eg
authors.forename: Rashika
authors.initials: R
authors.lastname: El Ridi
authors.forename: Hatem
authors.initials: H
authors.lastname: Tallima
authors.forename: Shadia H
authors.initials: SH
authors.lastname: Mohamed
authors.forename: Mona
authors.initials: M
authors.lastname: Montash
mesh_terms: D000042:Absorption; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity / Q000187:drug effects / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody / Q000187:drug effects / Q000276:immunology; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D003505:Cyclodextrins / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D007814:Larva / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D008168:Lung / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D047392:beta-Cyclodextrins
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol