title: [Normalization of histomorphometric and biomechanical parameters through metabolic control of fracture healing in spontaneously diabetic BB/OK rats].
abstract: OBJECTIVE
  The objective of this study was to examine changes in the histomorphometrical and mechanical parameters of bone during fracture healing in dependence on the diabetic metabolic state in spontaneously diabetic BB/O(ttawa)K(arlsburg) rats.
  
  
  METHODS
  Based on blood glucose values at the time of surgery, postoperative blood glucose course and postoperative insulin requirements, 90 spontaneously diabetic BB/OK rats were divided into groups with well compensated or poorly compensated metabolic states. Forty-five LEW.1A rats served as the normoglycemic controls. The femurs were fractured in a standardized procedure and then allowed to heal for 2, 4 and 6 weeks.
  
  
  RESULTS
  In terms of bone histomorphometry, our study showed that, within the first four weeks after fracture, severe mineralization disorders occurred exclusively in the rats with poorly compensated diabetic metabolic states, with a significant decrease of all fluorochrome- based parameters of mineralization, apposition, formation and a significantly prolonged timing of mineralization, as well as significantly decreased values of biomechanical properties in comparison to the spontaneously diabetic rats with well compensated metabolic states and to the control rats.
  
  
  CONCLUSIONS
  With a strictly controlled insulin therapy and a resulting well compensated diabetic metabolic state, severe mineralization disorders and the decreased values of biomechanical properties in the fracture repair in the poorly compensated diabetic metabolic state of the experimental animal can be avoided.
authors.affiliation: Forschungslabor der Klinik und Poliklinik für Orthopädie und Orthopädische Chirurgie, Ernst-Moritz-Arndt-Universität Greifswald. follak@uni-greifswald.de
authors.forename: N
authors.initials: N
authors.lastname: Follak
authors.forename: I
authors.initials: I
authors.lastname: Klöting
authors.forename: H
authors.initials: H
authors.lastname: Merk
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy*; D005264:Femoral Fractures / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology*; D007328:Insulin / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D012720:Severity of Illness Index; D016896:Treatment Outcome
chemical_list: D007328:Insulin