Electrical detection of single viruses.
We report direct, real-time electrical detection of single virus particles with high selectivity by using nanowire field effect transistors. Measurements made with nanowire arrays modified with antibodies for influenza A showed discrete conductance changes characteristic of binding and unbinding in the presence of influenza A but not paramyxovirus or adenovirus. Simultaneous electrical and optical measurements using fluorescently labeled influenza A were used to demonstrate conclusively that the conductance changes correspond to binding/unbinding of single viruses at the surface of nanowire devices. pH-dependent studies further show that the detection mechanism is caused by a field effect, and that the nanowire devices can be used to determine rapidly isoelectric points and variations in receptor-virus binding kinetics for different conditions. Lastly, studies of nanowire devices modified with antibodies specific for either influenza or adenovirus show that multiple viruses can be selectively detected in parallel. The possibility of large-scale integration of these nanowire devices suggests potential for simultaneous detection of a large number of distinct viral threats at the single virus level.
Patolsky
Fernando
F
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Zheng
Gengfeng
G
Hayden
Oliver
O
Lakadamyali
Melike
M
Zhuang
Xiaowei
X
Lieber
Charles M
CM
D000818:Animals; D001717:Birds; D004553:Electric Conductivity; D007120:Immunochemistry; D009980:Influenza A virus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010252:Paramyxoviridae / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012825:Silicon / Q000737:chemistry
D012825:Silicon