title: Chlorothalonil-biotransformation by glutathione S-transferase of Escherichia coli.
abstract: It has recently been reported that one of the most important factors of yeast resistance to the fungicide chlorothalonil is the glutathione contents and the catalytic efficiency of glutathione S-transferase (GST) (Shin et al, 2003). GST is known to catalyze the conjugation of glutathione to a wide variety of xenobiotics, resulting in detoxification. In an attempt to elucidate the relation between chlorothalonil-detoxification and GST, the GST of Escherichia coli was expressed and purified. The drug-hypersensitive E. coli KAM3 cells harboring a plasmid for the overexpression of the GST gene can grow in the presence of chlorothalonil. The purified GST showed chlorothalonil-biotransformation activity in the presence of glutathione. Thus, chlorothalonil is detoxified by the mechanism of glutathione conjugation catalyzed by GST.
authors.affiliation: Institute of Agricultural Science & Technology , Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Young-Mog
authors.initials: YM
authors.lastname: Kim
authors.forename: Kunbawui
authors.initials: K
authors.lastname: Park
authors.forename: Soon-Hyun
authors.initials: SH
authors.lastname: Jung
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Won-Chan
authors.initials: WC
authors.lastname: Kim
authors.forename: Gil-Jae
authors.initials: GJ
authors.lastname: Joo
authors.forename: In-Koo
authors.initials: IK
authors.lastname: Rhee
mesh_terms: D001711:Biotransformation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D009570:Nitriles / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D009570:Nitriles; D011994:Recombinant Proteins; D005982:Glutathione Transferase; D005978:Glutathione; C005806:tetrachloroisophthalonitrile