Whole-blood counting immunoassay as a short-turnaround test for detection of hepatitis B surface antigen, anti-hepatitis C virus antibodies, and anti-Treponema pallidum antibodies.
Whole-blood samples were used for a counting immunoassay (CIA) with the aim of developing a short- turnaround test. After optimization of the CIA, hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibodies (anti-HCV), and anti-Treponema pallidum antibodies (anti-TP) were detected as efficiently as by an enzyme immunoassay (EIA) with serum samples. The correlations between whole-blood CIA and serum EIA were 99.8, 97.1, and 99.4% for HBsAg, anti-HCV, and anti-TP, respectively. Whole-blood CIA may be of value when rapid screening of many samples is required.
Kudo
Toyoichiro
T
Deaprtment of Laboratory Medicine, Graduate School of Medicine, Kyoto University Hospital, 54 Kawahara Shogoin, Sakyoku, Kyoto 606-8507, Japan. tkudo@kuhp.kyoto-u.ac.jp
Kido
Aiko
A
Nishiyama
Yukiko
Y
Koganeya
Hiroshi
H
Okuda
Takako
T
Nabeshima
Motoshige
M
Iinuma
Yoshitsugu
Y
Ichiyama
Satoshi
S
D000907:Antibodies, Bacterial / Q000097:blood*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007124:Immunoenzyme Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens