title: Pichia anomala and Kluyveromyces wickerhamii killer toxins as new tools against Dekkera/Brettanomyces spoilage yeasts.
abstract: Two yeast killer toxins active on spoilage yeasts belonging to the genus Dekkera/Brettanomyces are here described for the first time. The two toxins produced by Pichia anomala (DBVPG 3003) and Kluyveromyces wickerhamii (DBVPG 6077), and named Pikt and Kwkt, respectively, differ for molecular weight and biochemical properties. Interestingly, the fungicidal effect exerted by Pikt and Kwkt against Dekkera bruxellensis is stable for at least 10 days in wine. Thus, a potential application for the two toxins as antimicrobial agents active on Dekkera/Brettanomyces during wine ageing and storage can be hypothesised.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Comitini
authors.forename: Jessica
authors.initials: J
authors.lastname: De Ingeniis
authors.forename: Jessica
authors.initials: J
authors.lastname: Ingeniis De
authors.forename: Laura
authors.initials: L
authors.lastname: Pepe
authors.forename: Ilaria
authors.initials: I
authors.lastname: Mannazzu
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ciani
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015169:Colony Count, Microbial; D005516:Food Microbiology; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007716:Kluyveromyces / Q000378:metabolism*; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000378:metabolism; D010843:Pichia / Q000378:metabolism*; D004718:Saccharomycetales / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D014920:Wine / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D006131:Growth Inhibitors; D009183:Mycotoxins; D010447:Peptide Hydrolases