Production of stably transfected cell lines using immunoporation.
Previous work from this laboratory has shown that immunoporation has the potential for the selective transfection of a range of different animal cells based on their immunological identity. The unique ability of immunoporation to target cells for transfection combined with the high efficiency of transfection and the high viability of cells make this method extremely promising for scientific and medical research. The experiments reported here show that not only can immunoporation produce transient transfection but also stably transfected cells are produced and such types of cells will be essential for the use of this method for gene therapy.
Tzavelas
Christos
C
University of Essex, Colchester, UK.
Bildirici
Lale
L
Rickwood
David
D
D002463:Cell Membrane Permeability; D003584:Cytological Techniques / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D008863:Microspheres; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods*
D011994:Recombinant Proteins