Heparin conjugated polylactide as a blood compatible material.
A heparin-conjugated biodegradable polymer (PLA-heparin) by the direct coupling of heparin to polylactide (PLA) was synthesized and characterized. The surface exposed heparin content associated PLA-heparin was measured to be 0.067 microg/cm2. PLA-heparin coated surface has shown higher hydrophilicity rather than control PLA surface. The clotting time of PLA-heparin conjugate measured by activated partial thromboplastin time (APTT) was significantly prolonged as compared to PLA. The bioactivity of bound heparin measured by APTT corresponds to 17.4% of free heparin. It has been also demonstrated that the conjugation of heparin suppresses the protein adsorption as well as the platelet adhesion. These results indicate that the unique property of bound heparin has an inhibiting influence on the coagulation, plasma protein adsorption, and subsequent platelet adhesion systems. This novel PLA-heparin conjugate could be applied as blood/tissue compatible biodegradable materials for implantable medical devices and tissue engineering.
Jee
Kyoung Soo
KS
Department of Molecular Science and Technology, Ajou University, Suwon 442-749, Korea.
Park
Hyung Dal
HD
Park
Ki Dong
KD
Kim
Young Ha
YH
Shin
Jung-Woog
JW
D001672:Biocompatible Materials / Q000032:analysis / Q000138:chemical synthesis*; D006493:Heparin / Q000032:analysis / Q000097:blood* / Q000138:chemical synthesis*; D006801:Humans; D010314:Partial Thromboplastin Time / Q000706:statistics & numerical data; D010973:Platelet Adhesiveness / Q000502:physiology; D011091:Polyesters / Q000032:analysis / Q000138:chemical synthesis*
D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D006493:Heparin