title: [Construction of a tRNAVal promoter plasmid expressing shRNAs in mammalian cells which mediates RNA interference].
abstract: OBJECTIVE
  Constructing a plasmid containing tRNAVal promoter to express shRNA which mediates RNA interference.
  
  
  METHODS
  A tRNAVal gene was amplified from human genomic DNA by PCR and replaced the last several bases of 3' end by a linker. The tRNAVal promoter after artificial mutation followed a shRNA sequence to luciferase was cloned into pUC18, Puc-tRNAVal, lucRi Cotransfected with pMAMneoLuc into BHK-21 cell to detect the effect of luciferase expression.
  
  
  RESULTS
  pUC-tRNAVallucRi suppressed the luciferase expression from pMAMneoLuc by 97.9%-9.5%.
  
  
  CONCLUSIONS
  The results showed that the tRNAVal shRNA plasmid could efficiently suppress luciferase expression in BHK-21.
authors.affiliation: Department of Geriatristic Cardiology, PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Lu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Yuan
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Peng
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Wu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012343:RNA, Transfer / Q000235:genetics*
chemical_list: D012343:RNA, Transfer; D008156:Luciferases