title: Glucose-stimulated insulin response in pregnant sheep following acute suppression of plasma non-esterified fatty acid concentrations.
abstract: BACKGROUND
  Elevated non-esterified fatty acids (NEFA) concentrations in non-pregnant animals have been reported to decrease pancreatic responsiveness. As ovine gestation advances, maternal insulin concentrations fall and NEFA concentrations increase. Experiments were designed to examine if the pregnancy-associated rise in NEFA concentration is associated with a reduced pancreatic sensitivity to glucose in vivo. We investigated the possible relationship of NEFA concentrations in regulating maternal insulin concentrations during ovine pregnancy at three physiological states, non-pregnant, non-lactating (NPNL), 105 and 135 days gestational age (dGA, term 147+/- 3 days).
  
  
  METHODS
  The plasma concentrations of insulin, growth hormone (GH) and ovine placental lactogen (oPL) were determined by double antibody radioimmunoassay. Insulin responsiveness to glucose was measured using bolus injection and hyperglycaemic clamp techniques in 15 non-pregnant, non-lactating ewes and in nine pregnant ewes at 105 dGA and near term at 135 dGA. Plasma samples were also collected for hormone determination. In addition to bolus injection glucose and insulin Area Under Curve calculations, the Mean Plasma Glucose Increment, Glucose Infusion Rate and Mean Plasma Insulin Increment and Area Under Curve were determined for the hyperglycaemic clamp procedures. Statistical analysis of data was conducted with Students t-tests, repeated measures ANOVA and 2-way ANOVA.
  
  
  RESULTS
  Maternal growth hormone, placental lactogen and NEFA concentrations increased, while basal glucose and insulin concentrations declined with advancing gestation. At 135 dGA following bolus glucose injections, peak insulin concentrations and insulin area under curve (AUC) profiles were significantly reduced in pregnant ewes compared with NPNL control ewes (p < 0.001 and P < 0.001, respectively). In hyperglycaemic clamp studies, while maintaining glucose levels not different from NPNL ewes, pregnant ewes displayed significantly reduced insulin responses and a maintained depressed insulin secretion. In NPNL ewes, 105 and 135 dGA ewes, the Glucose Infusion Rate (GIR) was constant at approximately 5.8 mg glucose/kg/min during the last 40 minutes of the hyperglycaemic clamp and the Mean Plasma Insulin Increment (MPII) was only significantly (p < 0.001) greater in NPNL ewes. Following the clamp, NEFA concentrations were reduced by approximately 60% of pre-clamp levels in all groups, though a blunted and suppressed insulin response was maintained in 105 and 135 dGA ewes.
  
  
  CONCLUSIONS
  Results suggest that despite an acute suppression of circulating NEFA concentrations during pregnancy, the associated steroids and hormones of pregnancy and possibly NEFA metabolism, may act to maintain a reduced insulin output, thereby sparing glucose for non-insulin dependent placental uptake and ultimately, fetal requirements.
authors.affiliation: Commonwealth Scientific and Industrial Research Organization, Division of Animal Production, Prospect, NSW Australia. Tim.regnault@uchsc.edu
authors.forename: Timothy R H
authors.initials: TR
authors.lastname: Regnault
authors.forename: Hutton V
authors.initials: HV
authors.lastname: Oddy
authors.forename: Colin
authors.initials: C
authors.lastname: Nancarrow
authors.forename: Nadarajah
authors.initials: N
authors.lastname: Sriskandarajah
authors.forename: Rex J
authors.initials: RJ
authors.lastname: Scaramuzzi
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001835:Body Weight / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000037:antagonists & inhibitors* / Q000097:blood*; D005260:Female; D005865:Gestational Age; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D015309:Glucose Clamp Technique / Q000379:methods; D013006:Growth Hormone / Q000097:blood; D006943:Hyperglycemia / Q000378:metabolism; D007262:Infusions, Intravenous / Q000379:methods; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D007774:Lactation / Q000502:physiology; D010928:Placental Lactogen / Q000097:blood / Q000378:metabolism; D011247:Pregnancy; D034561:Sheep, Domestic / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D013006:Growth Hormone; D010928:Placental Lactogen; D005947:Glucose