[Determination of clonidine in rabbit plasma by HPLC-MS].
OBJECTIVE
To determine clonidine in rabbit plasma by LC-MS.


METHODS
The LC-MS system consisted of Waters Alliance 2790 HPLC and Micromass ZQ-4000 MS. The HPLC was performed by using XTerra C18 (150 mm x 2.1 mm ID, 5 microm). The mobile phase, consisting of acetonitrile/ammonium hydrogen carbonate solution, was maintained to a flow-rate of 0.2 mL x min(-1) and the linear gradient elution was adopted. Mass spectrum was obtained by using electrospray ionization interface and the m/z of SIM was 230.


RESULTS
The average recovery was high and the method was reproducible. The calibration curve showed good linearity in the range of 1 - 80 microg x L(-1), the lowest limit of detection was 0.05 microg x L(-1). The Cmax, AUC0-t, and Tmax value of the pharmacokinetics parameter were (27 +/- 9) microg x L(-1), (5,352 +/- 1,121) microg x L(-1), (79 +/- 17) h.


CONCLUSIONS
The results demonstrated that the method had high sensitivity, good selectivity, accuracy and precision. It is used to determine the clonidine concentration in plasma. The transdermal patch can deliver clonidine to the surface of rabbit skin stably for periods of up to 1 week after a single application.
Ke
Guang-ming
GM
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Zhang
En-hong
EH
Wang
Li
L
Zhang
Qiang
Q
Du
Hong-guang
HG
Guo
Hong-you
HY
D000279:Administration, Cutaneous; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003000:Clonidine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D000959:Antihypertensive Agents; D003000:Clonidine