AglZ is a filament-forming coiled-coil protein required for adventurous gliding motility of Myxococcus xanthus.
The aglZ gene of Myxococcus xanthus was identified from a yeast two-hybrid assay in which MglA was used as bait. MglA is a 22-kDa cytoplasmic GTPase required for both adventurous and social gliding motility and sporulation. Genetic studies showed that aglZ is part of the A motility system, because disruption or deletion of aglZ abolished movement of isolated cells and aglZ sglK double mutants were nonmotile. The aglZ gene encodes a 153-kDa protein that interacts with purified MglA in vitro. The N terminus of AglZ shows similarity to the receiver domain of two-component response regulator proteins, while the C terminus contains heptad repeats characteristic of coiled-coil proteins, such as myosin. Consistent with this motif, expression of AglZ in Escherichia coli resulted in production of striated lattice structures. Similar to the myosin heavy chain, the purified C-terminal coiled-coil domain of AglZ forms filament structures in vitro.
Yang
Ruifeng
R
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844, USA.
Bartle
Sarah
S
Otto
Rebecca
R
Stassinopoulos
Angela
A
Rogers
Matthew
M
Plamann
Lynda
L
Hartzell
Patricia
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009068:Movement; D009218:Myosins / Q000235:genetics; D016941:Myxococcus xanthus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016366:Open Reading Frames; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018840:HSP70 Heat-Shock Proteins; D011994:Recombinant Proteins; C115004:SglK protein, Myxococcus xanthus; C071728:MglA protein, Myxococcus xanthus; D009218:Myosins