Genetic and expression analyses of FZD3 in schizophrenia.
BACKGROUND
Wnt signaling plays important roles in neurodevelopmental processes. Frizzled is a receptor of Wnt protein, and the Frizzled 3 (FZD3) gene was recently reported to be associated with schizophrenia. Our study attempted to confirm associations between FZD3 and schizophrenia in Japanese family and case-control samples.


METHODS
Genetic associations were evaluated using family-based transmission tests (212 families, 643 subjects) and case--control analysis (540 schizophrenia patients, 540 control sample). Six single nucleotide polymorphisms (SNPs) on the FZD3 locus were genotyped, and levels of FZD3 mRNA expression in postmortem brains were examined.


RESULTS
Neither family- nor population-based studies supported associations between FZD3 and schizophrenia. FZD3 expression was unaltered in schizophrenic brains.


CONCLUSIONS
Although two prior studies have reported associations using limited numbers of SNPs on FZD3, our intensive study failed to support any major contribution of FZD3 to schizophrenia susceptibility.
Ide
Masayuki
M
Laboratory for Molecular Psychiatry, RIKEN Brain Science Institute, Wako-city, Saitama, Japan.
Muratake
Tatsuyuki
T
Yamada
Kazuo
K
Iwayama-Shigeno
Yoshimi
Y
Iwamoto
Kazuya
K
Takao
Hitomi
H
Toyota
Tomoko
T
Kaneko
Naoshi
N
Minabe
Yoshio
Y
Nakamura
Kazuhiko
K
Kato
Tadafumi
T
Mori
Norio
N
Asada
Takashi
T
Someya
Toshiyuki
T
Yoshikawa
Takeo
T
D000328:Adult; D001921:Brain / Q000378:metabolism; D016022:Case-Control Studies; D003001:Cloning, Molecular / Q000379:methods; D005192:Family Health; D005260:Female; D051157:Frizzled Receptors; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012559:Schizophrenia / Q000235:genetics* / Q000378:metabolism
C105574:FZD3 protein, human; D051157:Frizzled Receptors; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled