[Transferring of multiple insect-resisting genes into two-line genetic male sterile rice Pei'ai 64S by particle bombardment].
Transformation of two-line genetic male sterile indica rice variety Pei'ai 64S was conducted by particle bombardment. Three insect-resisting genes ligased into plasmid vector pKC-3 was introduced into calli derived from mature embryo and a total of 33 transgenic plants had been obtained. The PCR and Southern blot analysis of each different gene in R0 plants and also PCR analysis of each different gene in R1 plants showed the integration of three insect-resisting genes into the genome of transgenic plants which could stably pass down.
Fan
Qin
Q
Life Science Research Center of Zhongshan University, Guangzhou 510275.
Xu
Xin Ping
XP
Li
Bao Jian
BJ
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C445075:PIN2 protein, Solanum americanum; D010940:Plant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis