title: Polymerase chain reaction and restriction fragment length polymorphism analysis of the ITS2 region for differentiation of Brazilian Biomphalaria intermediate hosts of the Schistosoma mansoni.
abstract: We sequenced the internal transcribed spacer 2 of the ribosomal DNA (ITS2-DNAr) from the three Schistosoma mansoni intermediate hosts in Brazil: Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. Four restriction enzymes were used and HpaII provided simple species-specific profiles easily visualized in polyacrylamide gels. The use of ITS2 is advantageous as it provides a small fragment of 460 bp which may be easily amplified by PCR. In the current work, we showed that the amplification of ITS2-DNAr together with HpaII enzyme restriction is an auxiliary molecular tool for the morphological identification of such snails as well as for taxonomic and phylogenetic studies of neotropical planorbids.
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil.
authors.forename: Teofânia Heloísa Dutra Amorim
authors.initials: TH
authors.lastname: Vidigal
authors.forename: Kelly Grace
authors.initials: KG
authors.lastname: Magalhães
authors.forename: Omar dos Santos
authors.initials: Odos S
authors.lastname: Carvalho Od
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004199:Disease Vectors; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012550:Schistosoma mansoni
chemical_list: D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D004262:DNA Restriction Enzymes