title: Typing of pneumococcal surface protein A (PspA) in Streptococcus pneumoniae isolated during epidemiological surveillance in Brazil: towards novel pneumococcal protein vaccines.
abstract: Pneumococcal protein vaccine based on pneumococcal surface protein A (PspA) is in development with the potential to offer broad range of protection against different strains. We have investigated the frequency of PspA family 1 (Fam1) and family 2 (Fam2) proteins among Streptococcus pneumoniae recovered from ongoing surveillance in Brazil. Fam1 and Fam2 were expressed in comparable rates among 366 isolates, with the potential coverage of 94.3%. PspA families were not associated to age group or source of isolates. However, considering the significant tendency of increasing prevalence of Fam2 associated to widespread dissemination of the genetically-related resistant strains, the monitoring of the PspA families derived from population-based data may be necessary in the context of vaccine development.
authors.affiliation: Bacteriology Branch, Adolfo Lutz Institute (IAL), Secretary of Health of the State of São Paulo, CEP: 01246-902, SP, Brazil. brandi@ial.sp.gov.br
authors.forename: Maria Cristina C
authors.initials: MC
authors.lastname: Brandileone
authors.forename: Ana Lucia S S
authors.initials: AL
authors.lastname: Andrade
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Teles
authors.forename: Rosemeire C
authors.initials: RC
authors.lastname: Zanella
authors.forename: Teresa I
authors.initials: TI
authors.lastname: Yara
authors.forename: José Luis
authors.initials: JL
authors.lastname: Di Fabio
authors.forename: Susan K
authors.initials: SK
authors.lastname: Hollingshead
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D001826:Body Fluids / Q000382:microbiology; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007223:Infant; D008875:Middle Aged; D009305:Nasopharynx / Q000382:microbiology; D010403:Penicillin Resistance; D011008:Pneumococcal Infections / Q000382:microbiology*; D022242:Pneumococcal Vaccines / Q000737:chemistry / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D022242:Pneumococcal Vaccines; D022223:Vaccines, Subunit; C051592:pneumococcal surface protein A