Photon-modulated changes of cell attachments on poly(spiropyran-co-methyl methacrylate) membranes.
Spiropyran is a photoresponsive molecule, and nonionic spiropyran is reversibly changed by UV irradiation to a hydrophilic polar, zwitterionic merocyanine isomer, and back again by visible light irradiation. A copolymer of nitrobenzospiropyran and methyl methacrylate, poly(NSP-co-MMA) was used as a material with a photosensitive surface. UV irradiation of the photosensitive surface of poly(NSP-co-MMA)-coated glass plates decreased the water contact angles (11 +/- 1 degrees ) and increased diameter of a water drop relative to the unexposed surface. Light-induced detachment of platelets and mesenchymal stem (KUSA-A1) cells on poly(NSP-co-MMA)-coated glass plates was observed upon simple- and patterned-light irradiation, whereas no light-induced detachment of platelets and mesenchymal stem cells was observed on poly(methyl methacrylate)-coated glass plates. This is a result of the change from a closed nonpolar spiropyran to the polar zwitterionic merocyanine isomer induced by UV irradiation. Light-induced detachment of fibrinogen adsorbed on poly(NSP-co-MMA) coated glass plates was also observed in this investigation.
Higuchi
Akon
A
Department of Applied Chemistry, Seikei University, 3-3-1 Kichijoji Kitamachi, Musashino, Tokyo 180-8633, Japan. higuchi@ch.seikei.ac.jp
Hamamura
Ayu
A
Shindo
Yosuke
Y
Kitamura
Hanako
H
Yoon
Boo Ok
BO
Mori
Taisuke
T
Uyama
Taro
T
Umezawa
Akihiro
A
D000818:Animals; D001578:Benzopyrans / Q000737:chemistry / Q000528:radiation effects*; D001792:Blood Platelets / Q000166:cytology / Q000528:radiation effects; D002460:Cell Line; D005260:Female; D006801:Humans; D007211:Indoles; D008689:Methacrylates / Q000737:chemistry / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D009574:Nitro Compounds; D017785:Photons; D010973:Platelet Adhesiveness / Q000528:radiation effects; D013234:Stem Cells / Q000166:cytology / Q000528:radiation effects*; D013499:Surface Properties / Q000528:radiation effects
D001578:Benzopyrans; D007211:Indoles; D008689:Methacrylates; D009574:Nitro Compounds; C088184:spiropyran