title: Additional observations on the sporozoite transmission of Plasmodium knowlesi to monkeys.
abstract: Saimiri boliviensis monkeys were infected by the intravenous injection of 50 sporozoites of the H strain of Plasmodium knowlesi dissected from the salivary glands of Anopheles dirus mosquitoes; prepatent periods were 11, 12, 13, 13, 13, and 16 days. Sporozoites of P. knowlesi stored frozen for 7 days, 53 days, 20 mo, 7 yr and 7 mo, and 11 yr and 5 mo induced infections in Macaca mulatta monkeys with prepatent periods of 7, 6, 8, 10, and 7 days, respectively. After frozen storage for 11 yr and 5 mo, infections were induced in S. boliviensis with prepatent periods of 10-13 days.
authors.affiliation: Division of Parasitic Diseases and Animal Resources Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. wec1@cdc.gov
authors.forename: William E
authors.initials: WE
authors.lastname: Collins
authors.forename: JoAnn S
authors.initials: JS
authors.lastname: Sullivan
authors.forename: Douglas
authors.initials: D
authors.lastname: Nace
authors.forename: Tyrone
authors.initials: T
authors.lastname: Williams
authors.forename: Allison
authors.initials: A
authors.lastname: Williams
authors.forename: G Gale
authors.initials: GG
authors.lastname: Galland
authors.forename: John W
authors.initials: JW
authors.lastname: Barnwell
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology; D015925:Cryopreservation / Q000662:veterinary; D007303:Insect Vectors / Q000469:parasitology; D008253:Macaca mulatta / Q000469:parasitology*; D008288:Malaria / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D008992:Monkey Diseases / Q000469:parasitology / Q000635:transmission*; D016790:Plasmodium knowlesi / Q000472:pathogenicity*; D012453:Saimiri / Q000469:parasitology*