Controlled immobilization of membrane proteins to surfaces for fourier transform infrared investigations.
We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier transform infrared spectra of such self-assembled (sub)monolayers deliver important structural information of the membrane proteins and are suited to screen the function of cellular receptors.
Rigler
Per
P
Ulrich
Wolf-Peter
WP
Vogel
Horst
H
D008565:Membrane Proteins / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
D008565:Membrane Proteins; D009571:Nitrilotriacetic Acid