[Experimental study of Qiyeling Decoction inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice].
OBJECTIVE
To study the function of Qiyeling Decoction in inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice.


METHODS
Nude mice with transplanted A549 tumor were randomly divided into the untreated control group (group A), chemotherapy treated group (group B), chemotherapy plus Qiyeling Decoction treated group (group C), Qiyeling Decoction treated group (group D) and managed correspondingly. The tumor volume was measured and calculated into tumor weight. The apoptosis of tumor cells were examined using in situ cell apoptosis detection kit.


RESULTS
The tumor weight was lower obviously in groups B, C and D than that in group A (P<0.05). The apoptosis of tumor cells was lower obviously in groups B, and C than that in group D (P<0.05). Cells in group A appeared perfect differentiation during the early stage and apoptosis later.


CONCLUSIONS
Qiyeling Decoction can induce A549 cell apoptosis in nude mice.
Jin
Chang-Juan
CJ
Department of Integrated Traditional Chinese and Western Medicine, Shanghai Chest Hospital, Shanghai 200030, China.
Sha
Hui-Fang
HF
Zhao
Lan-Xiang
LX
Feng
Jiou-Xian
JX
Gu
Wei-Yong
WY
Shi
Zheng-Yu
ZY
D000230:Adenocarcinoma / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D008819:Mice, Nude; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
D004365:Drugs, Chinese Herbal