title: Cytotoxic constituents of Diadema setosum.
abstract: 5,8-Epidioxycholest-6-en-3-ol (1), cholesterol (2), glycerol 1-palmitate (3) and glycerol 1,3-dioleate-2-stearate (4) were isolated from the methanol extract of the sea urchin Diadema setosum, which was collected from the Halong sea, Vietnam. Chemical structures were established based on extensive 1D, 2D-NMR, FAB-MS, El-MS spectroscopic data and GC-MS analysis. The NMR spectral data of compound 1 were reassigned by using HMQC and HMBC. Compound 1 was found to have strong cytotoxic effect against various cancer cell lines, such as KB (IC50, 2.0 microg/mL), FL (ICso, 3.93 microg/mL), and Hep-2 (IC50, 2.4 microg/mL) by in vitro assay.
authors.affiliation: Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam.
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Le Mai
authors.initials: le M
authors.lastname: Huong
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012617:Sea Urchins / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents