title: Photoresponsive nanogels formed by the self-assembly of spiropyrane-bearing pullulan that act as artificial molecular chaperones.
abstract: Novel photoresponsive nanogels were prepared by the self-assembly of spiropyrane-bearing pullulan (SpP). The solution properties of the nanogels could be controlled by photostimulation via isomerization between hydrophobic spiropyrane and hydrophilic merocyanine. The molecular chaperone-like activity of the nanogels in protein refolding was investigated. The activity of citrate synthase significantly increased when the amphiphilicity of SpP nanogels was switched by photostimulation.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida-Hommachi, Sakyo-ku, 606-8501, Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Hirakura
authors.forename: Yuta
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoyama
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D001578:Benzopyrans / Q000032:analysis / Q000138:chemical synthesis*; D005936:Glucans / Q000032:analysis / Q000138:chemical synthesis*; D007211:Indoles; D008027:Light; D018832:Molecular Chaperones / Q000032:analysis / Q000138:chemical synthesis*; D000080385:Nanogels; D009574:Nitro Compounds; D010775:Photic Stimulation / Q000379:methods*; D011092:Polyethylene Glycols / Q000032:analysis / Q000138:chemical synthesis*; D011094:Polyethyleneimine / Q000032:analysis / Q000138:chemical synthesis*; D012542:Scattering, Radiation
chemical_list: D001578:Benzopyrans; D005936:Glucans; D007211:Indoles; D018832:Molecular Chaperones; D000080385:Nanogels; D009574:Nitro Compounds; C413692:polyethylene glycol polyethyleneimine nanogel; C088184:spiropyran; D011092:Polyethylene Glycols; C009109:pullulan; D011094:Polyethyleneimine