Cis-acting effect of the IL1B C-31T polymorphism on IL-1 beta mRNA expression.
Since the effect of IL1B polymorphisms on IL-1beta production is still controversial, we selected two polymorphisms to test their cis-acting effect on IL-1beta mRNA expression by means of the allele-specific transcript quantification and the haplotype analysis. As for the C-31T polymorphism, we found that expression of the -31T allele was 2.2 times of the -31C allele. This higher transcription efficiency may correspond to the fact that C-31T is located in a TATA box. The other polymorphism, C+3954T, did not alter the levels of transcription. The use of the allele-specific transcript quantification enables us to exclude trans-acting effects of polymorphisms on the gene expression and contributes to understanding the roles of the IL1B polymorphisms in susceptibility to multifactorial diseases.
Kimura
R
R
Unit of Human Biology and Genetics, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Nishioka
T
T
Soemantri
A
A
Ishida
T
T
D002460:Cell Line; D016001:Confidence Intervals; D005786:Gene Expression Regulation / Q000276:immunology; D005816:Genetic Complementation Test / Q000706:statistics & numerical data; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D018709:Statistics, Nonparametric
D007375:Interleukin-1; D012333:RNA, Messenger