title: Exogenous application of glucose induces aging in rat cerebral oligodendrocytes as revealed by alteration in telomere length.
abstract: To investigate aspects of aging on rat oligodendrocytes, cells of an oligodendrocyte cell line, so-called OLN-93, were cultured either in the presence or absence of glucose. Our data demonstrated that glucose-induced aging in vitro caused an elongation and thickening of cell processes and significantly increased the expression of netrin reflecting a more mature state of oligodendrocyte development. A possible age-inducing effect of glucose is also supported by the decrease of ras protein expression and shortening of telomeres in glucose-treated oligodendrocytes. The present study clearly shows that OLN-93 cells are an exciting and suitable model system for the investigation of age-inducing molecules and the analysis of signaling pathways involved in cerebral aging and degenerations.
authors.affiliation: Department of Chemistry and Applied Science, Institute of Pharmaceutical Science, Swiss Federal, Institute of Technology, Zürich, Switzerland.
authors.forename: Vasili
authors.initials: V
authors.lastname: Dabouras
authors.forename: Andrée
authors.initials: A
authors.lastname: Rothermel
authors.forename: Alexandra
authors.initials: A
authors.lastname: Reininger-Mack
authors.forename: Sascha L
authors.initials: SL
authors.lastname: Wien
authors.forename: Paul Gottlob
authors.initials: PG
authors.lastname: Layer
authors.forename: Andrea Anneliese
authors.initials: AA
authors.lastname: Robitzki
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D003470:Culture Media; D004247:DNA / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000378:metabolism; D000075388:Netrin-1; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016615:Telomere / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D025521:Tumor Suppressor Proteins; D018631:ras Proteins / Q000378:metabolism
chemical_list: D003470:Culture Media; D009414:Nerve Growth Factors; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1; D004247:DNA; D018631:ras Proteins; D005947:Glucose