title: Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial.
abstract: A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta2-glycoprotein I (beta2-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta2-GPI was also detected on all tested CVCs.
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Bjerketorp
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Rosander
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Nilsson
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Jacobsson
authors.affiliation: Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Frykberg
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D001672:Biocompatible Materials; D002352:Carrier Proteins / Q000378:metabolism; D002405:Catheterization, Central Venous; D005340:Fibrinogen / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology; D053482:beta 2-Glycoprotein I
chemical_list: D001426:Bacterial Proteins; D001672:Biocompatible Materials; D002352:Carrier Proteins; D006023:Glycoproteins; D019151:Peptide Library; C112405:Sbi protein, Staphylococcus aureus; D053482:beta 2-Glycoprotein I; D005340:Fibrinogen