[The influence of guanine nucleotides and protein kinase C on Ca2+ from intracellular stores of pigs oocytes stimulated by theophylline and cyclic AMP].
Effect of guanine nucleotides and protein kinase C on Ca2+ exit from intracellular stores of pig oocytes, stimulated by theophylline and dbcAMP, was investigated using fluorescent dye chlortetracycline. Effect of cAMP on Ca2+ exit from intracellular stores of pig oocytes was not associated with activation of protein kinase C. In calcium-free medium, cAMP does not stimulate Ca2+ exit from intracellular stores of pig oocytes treated with GDP. In the presence of GDP, inhibition of protein kinase C activates Ca2+ exit from intracellular stores of pig oocytes on the action of cAMP. These data suggest the existence of different effects of guanine nucleotides on Ca2+ exit from intracellular stores of pig oocytes stimulated by cAMP.
Denisenko
V Iu
VIu
Kuz'mina
T I
TI
D000818:Animals; D001692:Biological Transport; D003994:Bucladesine; D002118:Calcium / Q000378:metabolism*; D002751:Chlortetracycline; D005260:Female; D005456:Fluorescent Dyes; D006150:Guanine Nucleotides / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors; D011493:Protein Kinase C / Q000494:pharmacology*; D013552:Swine; D013806:Theophylline
D005456:Fluorescent Dyes; D006150:Guanine Nucleotides; D010726:Phosphodiesterase Inhibitors; D003994:Bucladesine; D013806:Theophylline; D011493:Protein Kinase C; D002118:Calcium; D002751:Chlortetracycline