title: Characterization of pC7 from Lactobacillus paraplantarum C7 derived from Kimchi and development of lactic acid bacteria--Escherichia coli shuttle vector.
abstract: A cryptic plasmid pC7 was characterized, which was isolated from Lactobacillus paraplantarum C7 derived from Kimchi, a traditional korean vegetable food. The plasmid pC7 is a circular molecule of 2,134 base-pairs in length with a G + C content of 38.5%. The nucleotide sequence analysis revealed the presence of an open reading frame encoding a putative 317 amino acids protein homologous to replication proteins RepA. Furthermore, a putative double and a single-strand origin were identified. Together with features of nucleotide sequences, the detection of single-stranded intermediate DNA in Lb. paraplantarum demonstrated that pC7 replicates via a rolling circle mechanism. A cloning vector for lactic acid bacteria was developed on the basis of the pC7 replicon, into which were inserted an erythromycin resistance gene as a marker, multiple cloning sites, and Escherichia coli ColE1 replication origin. E. coli and several species of Lactobacillus and Leuconostoc can be transformed with the resultant vector. Therefore, pC7 derivatives may be useful LAB-E. coli shuttle vectors, which are essential in engineering important strains in food fermentation.
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Suwon 449-701, Republic of Korea.
authors.forename: Woo Jung
authors.initials: WJ
authors.lastname: Park
authors.forename: Kwan Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Hae Choon
authors.initials: HC
authors.lastname: Chang
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D003086:Bacteriocin Plasmids / Q000737:chemistry / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D001937:Brassica / Q000382:microbiology*; D003001:Cloning, Molecular; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005285:Fermentation; D005822:Genetic Vectors / Q000235:genetics*; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial