title: [Cloning and analyzing of the cDNA sequence of CHS-A gene of Narcissus].
abstract: Chalcone synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids. The production of flower pigment is specifically regulated by the activity of CHS. We cloned the cDNA sequence of CHS-A gene from Narcissus by PCR and analyzed the coding sequence of gene. The result demonstrated that the sequence of the coding region was 1167bp, encoding a protein of 389 amino acid which was more than 80% homology with CHS of the other 8 plants, such as Nicotine abacus and Solana tuberosum.
authors.affiliation: The Key Laboratory for Cell Biology and Tumor Cell Engineering, Ministry of Education, Xiamen University, Xiamen 361005.
authors.forename: Yin Yi
authors.initials: YY
authors.lastname: Huang
authors.forename: Ming Shan
authors.initials: MS
authors.lastname: Shen
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Mu Zhuan
authors.initials: MZ
authors.lastname: Chen
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D031427:Narcissus / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; D000217:Acyltransferases; C020141:flavanone synthetase