title: cDNA cloning of translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) from the intertidal harpacticoid copepod Tigriopus japonicus.
abstract: We synthesized a cDNA library from the intertidal copepod Tigriopus japonicus, converted it to phagemids and sequenced expressed sequence tags (ESTs). Of these, Tigriopus translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) was further characterized. The Tigriopus TCTP/HRF gene encoded 172 amino acid residues and showed high similarity to Drosophila but moderate similarity to other annelids (e.g. Brugia, Wuchereria and C. elegans). The Tigriopus TCTP/HRF gene appeared in the same clade as the annelids. Here, we describe the analysis of the Tigriopus TCTP/HRF gene.
authors.affiliation: Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Young Ja
authors.initials: YJ
authors.lastname: Kim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Bok-Geon
authors.initials: BG
authors.lastname: Kim
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Park
authors.forename: Hyeung-Sin
authors.initials: HS
authors.lastname: Kim
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Jung
authors.forename: Tim D
authors.initials: TD
authors.lastname: Williams
authors.forename: Wonchoel
authors.initials: W
authors.lastname: Lee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D003001:Cloning, Molecular; D033342:Copepoda / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D000090324:Tumor Protein, Translationally-Controlled 1