title: An alternative approach for the estimation of equivalent temperature in gas chromatography.
abstract: The temperature at which the isothermal retention time (tRiso) is equal to the temperature-programmed retention time (tRTPGC) or the isothermal retention index (Iiso) is equal to the temperature-programmed retention index (ITPGC) is defined as the equivalent temperature (Teq). The Teq of one-, two-, three-, and four-step temperature-programmed gas chromatography (TPGC) of unsaturated fatty acid methyl esters (FAMEs) from Chinese mustard seed oil is calculated. All of the tR values obtained at the Teq (tRTeq) are very close to the tRTPGC. The highest difference for each chromatogram is less than 0.5%. The slight deviation may partly arrive from the difficulty in setting the desired carrier gas flow rate. Also, good agreement among equivalent chain length values determined by different methods is observed, including the graphical method at the Teq. Therefore, it is speculated that the proposed method may facilitate the GC identification of FAMEs as well as other organic compounds in TPGC by using the available isothermal retention index database.
authors.affiliation: Biochemical Technology Division, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi. Toongkru, Bangkok 10140 Thailand.
authors.forename: Kornkanok
authors.initials: K
authors.lastname: Aryusuk
authors.forename: Dararat
authors.initials: D
authors.lastname: Yensruang
authors.forename: Kanit
authors.initials: K
authors.lastname: Krisnangkura
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000737:chemistry
chemical_list: D004952:Esters; D005227:Fatty Acids