title: Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection.
abstract: The study of the metabolomics of primary metabolites using conventional chemical analyses requires a high-throughput method. Chemical derivatizations are a prerequisite for gas-chromatographic separation, and a large sample quantity is needed for liquid-chromatographic separation and nuclear magnetic resonance detection systems. Recently, we have developed a capillary electrophoresis-mass spectrometry (CE-MS) technology that can simultaneously quantify a large number of primary metabolites, using only a small quantity of samples, and without any chemical derivatizations. Parallel use of a capillary electrophoresis-diode array detector (CE-DAD) system further enables almost all water-soluble intracellular metabolites to be analyzed. We demonstrate, with rice leaves, a simple and rapid method of sample preparation for CE analysis; using this method, we have successfully measured the levels of 88 main metabolites involved in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, photorespiration, and amino acid biosynthesis.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0035, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.forename: Takaaki
authors.initials: T
authors.lastname: Nishioka
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D018698:Glutamic Acid / Q000302:isolation & purification / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D009005:Monosaccharides / Q000302:isolation & purification / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D012265:Ribonucleotides / Q000378:metabolism
chemical_list: D009005:Monosaccharides; D012265:Ribonucleotides; D018698:Glutamic Acid