title: Effects of platelet lysates on select bone cell functions.
abstract: Although platelet-rich plasma and platelet concentrates have been used to promote bone healing in orthopaedic and maxillofacial surgery, the underlying cellular-level mechanisms remain poorly understood. The present in vitro study investigated the effects of human platelet lysate (PL) on selected functions of cultured bone cells. Cells from 18-day-old fetal rat calvaria were isolated by a collagenase digestion procedure. PL was added at different concentrations on pre- or post-confluent cell stage. After 1 day, bone cell proliferation was maximal and half-maximal in the presence of PL from 3 x 10(8) and 0.5 x 10(8) platelets/ml, respectively. During 17 h, the number of bone cells traversing the scrape border of a scrape wound model increased by 16-fold in the presence of PL from 3 x 10(8) platelets/ml. The presence of PL from 3 x 10(8) platelets/ml in pre-confluent bone cell cultures for 48 h resulted in a threefold decrease of alkaline phosphatase (ALP) specific activity. In the case of confluent bone cells, the presence of PL (from 1 x 10(6) to 3 x 10(8) platelets/ml) for 11 days, the ALP specific activity and total calcium content decreased in a PL dose-dependent manner and reached a minimum in the presence of PL from 3 x 10(8) platelets/ml. In summary, short-term PL exposure (up to 24 h) promotes the proliferative and chemotactic bone cell functions while long-term PL exposure results in a decrease of both ALP activity and mineral formation. These data show that the soluble components contained in PL may affect the bone healing process by modulating differently bone cell functions.
authors.affiliation: Laboratoire de Recherches Orthopediques, UMR-CNRS 7052, Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Soffer
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Ouhayoun
authors.forename: Christine
authors.initials: C
authors.lastname: Dosquet
authors.forename: Alain
authors.initials: A
authors.lastname: Meunier
authors.forename: Fani
authors.initials: F
authors.lastname: Anagnostou
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002118:Calcium / Q000037:antagonists & inhibitors; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002474:Cell-Free System / Q000502:physiology; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002457:Cell Extracts; D000469:Alkaline Phosphatase; D002118:Calcium