Filtering of ineffective siRNAs and improved siRNA design tool.
BACKGROUND
Short interfering RNAs (siRNAs) can be used to suppress gene expression and possess many potential applications in therapy, but how to design an effective siRNA is still not clear. Based on the MPI (Max-Planck-Institute) basic principles, a number of siRNA design tools have been developed recently. The set of candidates reported by these tools is usually large and often contains ineffective siRNAs. In view of this, we initiate the study of filtering ineffective siRNAs.


RESULTS
The contribution of this paper is 2-fold. First, we propose a fair scheme to compare existing design tools based on real data in the literature. Second, we attempt to improve the MPI principles and existing tools by an algorithm that can filter ineffective siRNAs. The algorithm is based on some new observations on the secondary structure, which we have verified by AI techniques (decision trees and support vector machines). We have tested our algorithm together with the MPI principles and the existing tools. The results show that our filtering algorithm is effective.


BACKGROUND
The siRNA design software tool can be found in the website http://www.cs.hku.hk/~sirna/


BACKGROUND
smyiu@cs.hku.hk
Yiu
S M
SM
Department of Computer Science, University of Hong Kong, Hong Kong. smyiu@cs.hku.hk
Wong
Prudence W H
PW
Lam
T W
TW
Mui
Y C
YC
Kung
H F
HF
Lin
Marie
M
Cheung
Y T
YT
D000465:Algorithms; D001185:Artificial Intelligence; D001483:Base Sequence; D019985:Benchmarking / Q000379:methods; D017076:Computer-Aided Design; D005818:Genetic Engineering / Q000379:methods; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods*; D017423:Sequence Analysis, RNA; D012984:Software
D034741:RNA, Small Interfering