[Molecular study of Vibrio cholerae non-O1 isolated from zooplankton of São Marcos Bay/São Luis - MA, Brasil].
The study was developed to analyze the plasmidial DNA, research virulence genes and identify genetic diversity of 31 strains of Vibrio cholerae non-O1 isolated from zooplankton of the Bacanga and Anil rivers in São Luis-MA. The study of plasmidial DNA showed 2 or 3 plasmids from 10 strains between 5.5 and 40 kilobasis. There was only single ribotype pattern. PCR methods did not show the genes ctxA, ace and zot, while RADP-PCR identified genetic diversity in the strains, showing the potential for variability in this species.
Gonçalves
Eloisa da Graça do Rosario
Eda G
Departamento de Patologia, Universidade Federal do Maranhão, São Luis, MA. regionalsbmt@elo.com.br
Leal
Nilma Cintra
NC
Hofer
Ernesto
E
D000818:Animals; D001938:Brazil; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D010957:Plasmids / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D015048:Zooplankton / Q000382:microbiology*
D004269:DNA, Bacterial