title: [Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice].
abstract: BACKGROUND
  Construction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.
  
  
  METHODS
  gag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.
  
  
  RESULTS
  The replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.
  
  
  CONCLUSIONS
  Replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.
authors.affiliation: National Center for AIDS Prevention and Control. Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Zhou
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Zhang
authors.forename: Kun-sue
authors.initials: KS
authors.lastname: Hong
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000235:genetics*; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D014162:Transfection; D019444:Vaccines, DNA / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
chemical_list: D016915:AIDS Vaccines; D015685:Fusion Proteins, gag-pol; D015686:Gene Products, env; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1