Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10.
Macrophages co-incubated with Candida albicans strain CR1 in vitro showed early signs of apoptosis, but evolved to necrosis after 2 h. In this study, we investigated whether strain CR1 caused apoptosis or necrosis of macrophages after its inoculation into mice peritoneal cavity, and whether this correlated with the secretion of IL-10. Peritoneal macrophages from mice that received an inoculum of C. albicans CR1 showed signs of apoptosis and necrosis from 30 min to 2 h afterwards, whereas heat-killed C. albicans did not cause those effects. IL-10 production was low during the first 6 h post-infection, when macrophages predominated in the peritoneal exudate, whereas its higher production after 24 h correlated with an increase of neutrophils in the exudate. Treatment of CR1 with pepstatin (an inhibitor of proteinases) prevented the process of apoptosis and significantly reduced IL-10 production, suggesting that the increased production of IL-10 was caused by processes occurring during the initial phase of infection, such as apoptosis, necrosis and uptake of death cells.
Gasparoto
Thaís Helena
TH
Departamento de Ciências Patológicas, CCB, Universidade Estadual de Londrina, Cx P. 6001, 86051-990 Londrina, Brazil.
Gaziri
Luis Carlos Jabur
LC
Burger
Eva
E
de Almeida
Ricardo Sérgio Couto
RS
Felipe
Ionice
I
D000818:Animals; D017209:Apoptosis; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D016753:Interleukin-10 / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D008297:Male; D051379:Mice
D016753:Interleukin-10