title: Multiple Chromosome Abnormalities Following Bone Marrow Transplant for Chronic Myelogenous Leukemia.
abstract: A 44-year-old female was diagnosed in the chronic phase of chronic myelogenous leukemia (CML) and was confirmed to be Philadelphia chromosome positive by a bone marrow cytogenetic study. No additional cytogenetic abnormalities were found. The patient's cell counts were initially well controlled with hydrox-yurea. She then received an unrelated 6 of 6 HLA matched allo-geneic bone marrow transplant (BMT) from a male donor. The patient underwent myeloablative therapy with thiotepa and five fractions of total body radiation prior to the transplant. About four weeks after transplantation, the patient developed biopsy-proven graft-versus-host disease of the skin and GI tract. A blood sample was drawn at that time for cytogenetic analysis. Among 34 analyzed cells, 22 were normal male donor cells. The remaining 12 cells did not have the t(9;22), but had numerous structural abnormalities. While many cells were missing an X chromosome, other abnormalities, including deletions, rearrangements, dicentrics, acentric fragments, rings and marker chromosomes were non-clonal. No clinical evidence of progression from CML chronic phase was found, suggesting that the non-clonal abnor-malities were therapy related.
authors.affiliation: Department of Pathology, West Virginia University, P. O. Box 9203, Morgantown, WV 26506-9203. swenger@hsc.wvu.edu.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Ifti
authors.initials: I
authors.lastname: Ahmed
authors.forename: Solveig
authors.initials: S
authors.lastname: Ericson
authors.forename: Sharon
authors.initials: S
authors.lastname: Wenger