Separation of proteins on surface-modified poly(dimethylsiloxane) microfluidic devices.
Separation and detection of proteins have been realized on nonionic surfactant-modified poly(dimethylsiloxane) (PDMS) microfabricated devices with end-column amperometric detection. The hydrophobic PDMS channels are turned into hydrophilic ones after being modified with Brij35 and facilitate the separation of proteins. The coating can remarkably reduce the adsorption of large protein molecules and is stable in the range of pH 6-12. The detection of proteins in such channels needs less rinsing time and thus efficiency is raised. Even large molecules of proteins can also be detected with better reproducibility and enhanced plate numbers. The relative standard deviation (RSD) of the migration time for glucose oxidase (GOD) is 2.2% (n = 19). Separation of GOD and myoglobin has been developed in modified channels. Predominant operational variables, such as the coating conditions, the concentration of surfactant and buffer, are studied in detail.
Dou
Yue-Hua
YH
The State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Department of Chemistry, Nanjing University, Nanjing, P. R. China.
Bao
Ning
N
Xu
Jing-Juan
JJ
Meng
Fei
F
Chen
Hong-Yuan
HY
D004129:Dimethylpolysiloxanes; D004563:Electrochemistry / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D007202:Indicators and Reagents; D008832:Microchemistry; D046208:Microchip Analytical Procedures / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000379:methods; D009211:Myoglobin / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D007202:Indicators and Reagents; D009211:Myoglobin