title: Tumor necrosis factor-alpha is required for gastritis induced by Helicobacter felis infection in mice.
abstract: Helicobacter pylori colonizes the gastric mucosa of human and causes chronic gastritis. The previous studies have demonstrated that gamma interferon (IFN-gamma) but not tumor necrosis factor-alpha (TNF-alpha) plays a critical role in pathogenesis of gastritis induced by H. pylori infection. In this study we investigated the induction of gastritis induced by H. felis infection in TNF-alpha-deficient mice, comparing with IFN-gamma-deficient mice. The scores of gastritis and epithelial changes of TNF-alpha-deficient mice and IFN-gamma-deficient mice were significantly lower than that of C57BL/6 mice. Moreover, the degrees of gastritis and epithelial changes of TNF-alpha-deficient mice were rather low compared with that of IFN-gamma-deficient mice. In spleen cell cultures stimulated with heat-killed H. felis, IFN-gamma production by TNF-alpha-deficient mice and TNF-alpha production by IFN-gamma-deficient mice were significantly reduced compared with those in C57BL/6 mice. These results suggested that TNF-alpha is involved in pathogenesis of gastritis induced by H. felis infection as IFN-gamma and that an interaction between TNF-alpha and IFN-gamma might be required in pathogenesis of gastritis induced by Helicobacter infection.
authors.affiliation: Department of Bacteriology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Tomisato
authors.initials: T
authors.lastname: Miura
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Saito
authors.forename: Hiroo
authors.initials: H
authors.lastname: Madarame
authors.forename: Kenji
authors.initials: K
authors.lastname: Sekikawa
authors.forename: Yoh-Ichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Iwakura
authors.forename: Akio
authors.initials: A
authors.lastname: Nakane
mesh_terms: D000818:Animals; D005260:Female; D005756:Gastritis / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D044922:Helicobacter felis / Q000472:pathogenicity*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma