Artificial receptor-attached amphiphilic copolymer for barbiturate binding in aqueous media.
A water-soluble self-associating amphiphilic copolymer was employed to provide a microenvironment for the solvation of a hydrogen-bonding barbiturate artificial receptor, to facilitate molecular recognition in water. The receptor-attached amphiphilic polymer (RP) was synthesized through random copolymerization of 3% (mol) barbiturate receptor-monomer, 70% (mol) 3-sulfopropyl methacrylate, and 27% (mol) n-dodecyl acrylate. Difference UV spectra of pH 6.5 aqueous solutions of phenobarbital and receptor-polymer (RP) gave peaks and valleys at 272 and 301 nm respectively, consistent with binding characteristics of monomeric barbiturate receptors in chloroform. Specific association between phenobarbital and the receptor-polymer was further indicated based on investigations of a receptor-free control polymer (CP) of similar polar/nonpolar monomer ratio. Micellar electrokinetic chromatography was applied for studying polymer-phenobarbital association, by capillary electrophoresis.
Loizidou
Eriketi
E
Department of Chemistry, San Diego State University, 5500 Campanile Drive, California 92182, USA. loizidou@rohan.sdsu.edu
Zeinalipour-Yazdi
Constantinos
C
Sun
Lifang
L
D001463:Barbiturates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D011963:Receptors, GABA-A / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000032:analysis
D001463:Barbiturates; D011108:Polymers; D011963:Receptors, GABA-A; D013501:Surface-Active Agents; C030975:barbiturate receptor; D014867:Water