Optimal fermentation conditions for enhanced glutathione production by Saccharomyces cerevisiae FF-8.
The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
Cha
Jae-Young
JY
Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Park
Jin-Chul
JC
Jeon
Beong-Sam
BS
Lee
Young-Choon
YC
Cho
Young-Su
YS
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D003545:Cysteine / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism; D017680:Potassium Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry
D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; C013216:potassium phosphate; D005978:Glutathione; D005947:Glucose; D003545:Cysteine