The effect of xylanase on lignocellulosic components during the bleaching of wood pulps.
HPLC, SEM and XRD techniques have been proposed as methods for ascertaining the changes occurring in polysaccharides (cellulose and xylans) and fibres during the xylanase bleaching processes. TCF and ECF bleached pulps with and without enzyme pretreatment were analysed. The ratio of carbohydrates present in the pulp, observation of changes occurring in the surface of the fibres and the crystallinity and accessibility of the bleached fibres were determinated. These characteristics have been related with pulp properties. Xylan content decreased when pulp was bleached. Xylanase treatment substantially reduced the xylose content present in pulp, measured by HPLC after the hydrolysis method of the sample. Morphological changes in the fibres occurred when the enzymatic treatment was applied. Bleaching increased the crystallinity of the pulp and enzyme pretreatment also affected the crystallinity of cellulose fibres
Roncero
M Blanca
MB
Department of Textile and Paper Engineering, E.T.S.E.I.T. Polytechnical University of Catalonia. Colom 11, 08222 Terrassa, Spain. roncero@etp.upc.es
Torres
Antonio L
AL
Colom
José F
JF
Vidal
Teresa
T
D050260:Carbohydrate Metabolism; D002482:Cellulose / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005052:Eucalyptus / Q000648:ultrastructure; D007221:Industry / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010209:Paper; D014934:Wood; D014961:X-Ray Diffraction
C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases