title: Toxoplasma gondii-derived heat shock protein 70 stimulates the maturation of human monocyte-derived dendritic cells.
abstract: We investigated the role of Toxoplasma gondii-derived heat shock protein 70 (TgHSP70) as a dendritic cell (DC) maturation-inducing molecule. TgHSP70 induced the maturation of human monocyte-derived dendritic cells as determined by increased levels of surface markers, namely, CD40, CD80, CD86, and HLA-DR. Moreover, TgHSP70 also reduced phagocytic activity and increased the allostimulatory capacity of DCs, suggesting the functional maturation of DCs by TgHSP70. Maturation of DCs by TgHSP70 also elicited a significant increase in IL-12 production in a polymyxin B-insensitive manner. TgHSP70 also stimulated extracellular signal-regulated kinase and p38 kinase pathways in DCs, and TgHSP70-induced IL-12 production was inhibited by SB203580 but not by PD98059, thus indicating the role of p38 kinase in the maturation of DCs by TgHSP70. This study demonstrates the role of TgHSP70 in the functional maturation of DCs and suggests TgHSP70 as a useful molecule for the development of a vaccine against T. gondii.
authors.affiliation: Medical Research Center for Cancer Molecular Therapy, Dong-A University, Busan 602-714, Republic of Korea.
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Kang
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Youl-Nam
authors.initials: YN
authors.lastname: Lee
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Jo
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Kim
authors.forename: Fumie
authors.initials: F
authors.lastname: Aosai
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yano
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D015800:Protozoan Proteins / Q000494:pharmacology*; D014122:Toxoplasma / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D015800:Protozoan Proteins