Regulation of substance P mRNA expression in human dermal microvascular endothelial cells.
Vascular endothelial cells have been identified as a source of substance P (SP) which may act in an autocrine/paracrine fashion to bring about nitric oxide (NO)-dependent vasodilatation and mitogen-induced cell division or immunologic and inflammatory responses. Whilst SP is localised in and released from endothelial cells, an endothelial mRNA expression of SP has not previously been shown. In the present study, mRNA expression of SP in human dermal microvascular endothelial cells is demonstrated using in situ hybridisation techniques with enhancement procedures. Incubation of microvascular endothelial cells with nerve growth factor (NGF) under conditions of increased shear stress increases the mRNA expression and release of SP Endothelin (ET) release is also enhanced. These changes are pertinent to circulatory, events that may occur in Raynaud's phenomenon in systemic sclerosis.
Milner
P
P
Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, London, UK.
Bodin
P
P
Guiducci
S
S
Del Rosso
A
A
Kahaleh
M B
MB
Matucci-Cerinic
M
M
Burnstock
G
G
D018929:Cell Culture Techniques; D020405:Dermis / Q000098:blood supply / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D016232:Endothelins / Q000378:metabolism; D006801:Humans; D008833:Microcirculation / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D033081:Shear Strength; D013373:Substance P / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology
D016232:Endothelins; D012333:RNA, Messenger; D013373:Substance P; D020932:Nerve Growth Factor