title: Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori.
abstract: Translationally controlled tumor protein (Tctp/p23) is known to be synthesized preferentially in cells during the early growth phase of tumors, but is also expressed in normal cells. To elucidate its molecular basis of the expression and physiological significance, a cDNA encoding for the Bombyx mori Tctp (BmTctp) was deduced by editing the partial cDNA sequences registered in a Bombyx EST database. RT-PCR analyses indicated that the BmTCTP mRNA was transcribed in all larval organs examined and was present constantly during the cell cycle of BmN4 cells. A genomic clone of 4255 nucloetide residues produced by inverse PCR contained the 5'-flanking region, two introns and three exons of the BmTCTP gene. Sequence analysis of the 5'-flanking region indicated that a putative promoter region contains several canonical transcription elements such as GATA box, CCAAT motif, MEF2, E4BP4.01 and AP-1, but lacks a TATA box element. Luciferase reporter assay of the deletion constructs of the 5'-flanking region revealed that the -676 to +66 region enhanced the promoter activity the most markedly. In addition to this, there were at least two enhancer-like elements and several repressor elements.
authors.affiliation: Laboratory of Silkworm Science, Faculty of Agriculture, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Masateru
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.forename: Si-Kab
authors.initials: SK
authors.lastname: Nho
authors.forename: Katsumi
authors.initials: K
authors.lastname: Koga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005720:Gamma Rays; D023281:Genomics; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000528:radiation effects; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D000090324:Tumor Protein, Translationally-Controlled 1