title: Validation of ion chromatography for human salivary anionic analysis.
abstract: OBJECTIVE
  Assess the applicability of ion chromatography (IC) for human saliva studies.
  
  
  METHODS
  The behaviour of an ion chromatograph (DX-100, Dionex) in the analysis of human saliva was examined in the following respects: calibration, reproducibility, recovery, and effect of pre-treatment conditions (dilution, temperature). The eluents used were 1.7 mM NaHCO3 + 1.8 mM Na2CO3 + 100 mg/L p-cyanophenol (2 mL/min) and 1.6 mM perfluorobutyric acid + 0.3 mM sodium hydroxide (0.3 mL/min) for inorganic and organic anions, respectively.
  
  
  RESULTS
  The calibration curves were satisfactorily linear, but did not have unit slope (log-log plot) in the case of chloride, phosphate, sulphate and acetate. No significant effects were detected for centrifugation temperature, or between runs on the same occasion.
  
  
  CONCLUSIONS
  IC appears to be a valuable technique well-suited to the efficient analysis of several salivary anions.
authors.affiliation: Guanghua College of Stomatology, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Z-F
authors.initials: ZF
authors.lastname: Chen
authors.forename: B W
authors.initials: BW
authors.lastname: Darvell
authors.forename: V W-H
authors.initials: VW
authors.lastname: Leung
mesh_terms: D000838:Anions / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods; D013696:Temperature
chemical_list: D000838:Anions