title: In vitro regulation of Mac-1 expression on bovine polymorphonuclear leukocytes by endotoxin and tumor necrosis factor-alpha at different stages of lactation.
abstract: The purpose of this in vitro study is to clarify some of the underlying mechanisms leading to the decreased migratory capacity of polymorphonuclear leukocytes (PMN) during mastitis in dairy cows soon after calving. Surface expression of Mac-1 (CD11b, CR3) on PMN and of CD14 on monocytes was measured in early- (EL), peak- (PL), and midlactation (ML) by flow cytometric analysis. In addition, we evaluated the effect of lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-alpha on CD11b surface expression in PMN at different stages of lactation in a whole blood model. During EL, while resting monocytes expressed diminished levels of CD14, the basal expression of CD11b on PMN was not significantly altered. The relative increase of CD11b on PMN after incubation with LPS or TNF-alpha did not significantly differ among EL, PL, or ML at any of the concentrations tested. The current findings do not support an important role for basal CD11b levels nor for a defective mobilization of CD11b by LPS and TNF-alpha in the reduced migratory capacity of PMN during EL.
authors.affiliation: Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Araceli
authors.initials: A
authors.lastname: Diez-Fraile
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
authors.forename: Luc
authors.initials: L
authors.lastname: Duchateau
authors.forename: Max J
authors.initials: MJ
authors.lastname: Paape
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
mesh_terms: D000818:Animals; D002417:Cattle; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D007774:Lactation / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000187:drug effects / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000276:immunology; D008892:Milk / Q000166:cytology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha