title: Effect of H. pylori on COX-2 expression in gastric remnant after distal gastrectomy.
abstract: OBJECTIVE
  Helicobacter pylori infection is known to induce gastritis, oxidative stress, and cyclooxygenase (COX)-2 expression in the gastric mucosa. However, the effect of H. pylori infection on remnant gastritis has not been studied. We investigated whether the severity of remnant gastritis and COX-2 expression were affected by H. pylori infection after distal gastrectomy.
  
  
  METHODS
  The study included 97 patients with gastric cancer who underwent curative distal gastrectomy with lymphadenectomy in our department between May 1999 and April 2001. All patients underwent endoscopic examination 2 weeks before and 12 weeks after surgery. The presence of H. pylori infection was determined by urease activity, hematoxylin-eosin staining, and immunochemical staining. Histologic remnant gastritis was graded based on the degree of neutrophil infiltration using the updated Sydney System. COX-2 expression was estimated immunohistochemically.
  
  
  RESULTS
  Both the degree of neutrophil infiltration and the level of COX-2 expression were significantly higher in patients with than without H. pylori (p<0.05). There was a significant correlation between the degree of neutrophil infiltration and the degree of COX-2 expression (p<0.001).
  
  
  CONCLUSIONS
  H. pylori eradication may become a treatment for preventing both remnant gastritis as well as remnant gastric carcinoma after distal gastrectomy.
authors.affiliation: Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, Osaka, Japan. fuku@eva.hi-ho.ne.jp
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Fukuhara
authors.forename: Harushi
authors.initials: H
authors.lastname: Osugi
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Takada
authors.forename: Masashi
authors.initials: M
authors.lastname: Takemura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Lee
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Morimura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Taguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kaneko
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yushi
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Shoji
authors.initials: S
authors.lastname: Fukushima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D051546:Cyclooxygenase 2; D005260:Female; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000473:pathology; D018530:Gastric Stump / Q000473:pathology*; D005756:Gastritis / Q000473:pathology*; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis; D008197:Lymph Node Excision; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D020556:Neutrophil Infiltration; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D013274:Stomach Neoplasms / Q000601:surgery*
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases