Expression analysis of the pistil genes in controlling self-incompatibility of brassica campestris by CO2 gas using microarray.
In Brassica campestris, self-incompatibility (SI) can be overcome by CO2 gas treatment. Previously, we reported inhibition of the SI in the Brassica campestris line, 734, by CO2 gas. In this study, we used a cDNA microarray comprising 1,184 unique cDNA ESTs from a pistil-specific cDNA library of the Brassica line to examine the expression of the pistil genes in response to CO2. Microarray analysis revealed that expression of 2% of the pistil genes was altered by exposure to CO2 gas. Surprisingly, the SLG gene of the susceptible line was one of the down-regulated genes. Transmission electron microscopic analysis showed that the CO2 caused morphological changes in the papillary cell. Our results demonstrate that the overcoming of SI by CO2 gas involves reduced expression of the SLG gene in Brassica.
Kwun
Minjung
M
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Choi
Youngju
Y
Yoon
Hyejin
H
Park
Beom-Seok
BS
Kang
Bong-Joong
BJ
Chung
Yong-Yoon
YY
D001937:Brassica / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism*; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics* / Q000648:ultrastructure; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000502:physiology
D010940:Plant Proteins; D002245:Carbon Dioxide