title: Molecular survey of Mycoplasma haemofelis and 'Candidatus mycoplasma haemominutum' infection in cats in Yamaguchi and surrounding areas.
abstract: A molecular survey of hemoplasma (Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum') in Yamaguchi Prefecture and surrounding areas was performed by using molecular methods. PCR-RFLP with HindIII revealed that 2 cats were infected with M. haemofelis, and 16 with 'C. Mycoplasma haemominutum' among 102 randomly selected cats. Partial 16S rRNA gene sequences of M. haemofelis and 'C. Mycoplasma haemominutum' determined in this study showed percent similarities of 98.3-99.8% and 96.4-100%, respectively, with those from other countries. Hemoplasma infections were more frequently detected in free-roaming cats than inside cats. Also, the status of FeLV infection was another significant risk factor for hemoplasma infection.
authors.affiliation: Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Shoko
authors.initials: S
authors.lastname: Taroura
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Une
authors.forename: Munekazu
authors.initials: M
authors.lastname: Nakaichi
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
mesh_terms: D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology*; D002415:Cats; D016000:Cluster Analysis; D017931:DNA Primers; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012307:Risk Factors; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S