[DNA quantitative analysis of head and neck tumor].
OBJECTIVE
This research discussed the diagnostic value of cellular quantitative analysis in head and neck tumor.


METHODS
We analysis quantitative parameters of 23 malignant tumors and 20 benign diseases with automated DNA image cytometry, they are the DNA Index (DI), S-phase Fraction (SPF), Proliferation Index (PI) and 5c Exceeding (5c Ex).


RESULTS
The masculine rate of cellular quantitative analysis and conventional method are 76.92% and 50.00%. The value of SPF, PI and 5c Ex are 14.05%, 19.59% and 1.59% in malignant tumor, respectively. The parameters of SPF and PI showed distinct difference in the area of malignant tumor than the 1 cm sideward area of tumor and the edge of tumor.


CONCLUSIONS
Automatic DNA image cytometry has higher masculine rate of malignant tumor than conventional method in early detection of tumor. The cellular quantitative parameters, such as SPF, PI and 5c Ex, are able to guide clinical work, specially in diagnosing of head and neck tumor, estimating biological character of malignant tumor and evaluating the area of excision.
Wang
Tao
T
Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Huang
Hongyan
H
Cui
Yonghua
Y
Sun
Xiaorong
X
Liu
Qiurun
Q
Gao
Qixue
Q
D002467:Cell Nucleus / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D019044:Image Cytometry
D004273:DNA, Neoplasm