Comparison of multiplexed techniques for detection of bacterial and viral proteins.
Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.
Rao
Rupa S
RS
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Visuri
Steven R
SR
McBride
Mary T
MT
Albala
Joanna S
JS
Matthews
Dennis L
DL
Coleman
Matthew A
MA
D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D001407:Bacillus / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry; D017909:Levivirus / Q000378:metabolism; D008863:Microspheres; D040081:Protein Array Analysis / Q000379:methods*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001426:Bacterial Proteins; D014764:Viral Proteins