title: New targets of Arabidopsis thioredoxins revealed by proteomic analysis.
abstract: Proteomics was used to search for putative thioredoxin (TRX) targets in leaves of the model plant, Arabidopsis thaliana. About forty different proteins have been found to be reduced by TRX, after TRX itself has been specifically reduced by its NADPH-dependent reductase. Twenty-one of the identified proteins were already known or recently proposed to be TRX-dependent and nineteen of the proteins were new potential targets. The identified proteins are involved in a wide variety of processes, including the Calvin cycle, metabolism, photosynthesis, folding, defense against oxidative stress and amino acid synthesis. Two proteins from the glycine cleavage complex were also identified as putative TRX targets, and a new role can be postulated in leaves for TRX in defense against herbivores and/or pathogens.
authors.affiliation: IBBMC, CNRS, Université Paris-Sud, Orsay, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Marchand
authors.forename: Pierre
authors.initials: P
authors.lastname: Le Maréchal
authors.forename: Yves
authors.initials: Y
authors.lastname: Meyer
authors.forename: Myroslawa
authors.initials: M
authors.lastname: Miginiac-Maslow
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Issakidis-Bourguet
authors.forename: Paulette
authors.initials: P
authors.lastname: Decottignies
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013879:Thioredoxins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome; D013879:Thioredoxins