title: Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus.
abstract: Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. The enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and the immobilized toxins were probed with monoclonal antibodies. The antibodies 2A3 and 1A8 allowed the specific detection of the B component of HBL and the nheA component of NHE. The assay enabled the reliable identification of HBL expressing colonies and differentiation from NHE producing but HBL negative colonies.
authors.affiliation: Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, 85764 Oberschleissheim, Munich, Germany. m.moravek@mh.vetmed.uni-muenchen.de
authors.forename: Maximilian
authors.initials: M
authors.lastname: Moravek
authors.forename: Monika
authors.initials: M
authors.lastname: Wegscheider
authors.forename: Anja
authors.initials: A
authors.lastname: Schulz
authors.forename: Richard
authors.initials: R
authors.lastname: Dietrich
authors.forename: Christine
authors.initials: C
authors.lastname: Bürk
authors.forename: Erwin
authors.initials: E
authors.lastname: Märtlbauer
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001409:Bacillus cereus / Q000302:isolation & purification* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology; D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D006460:Hemolysin Proteins; D006461:Hemolysis; D015151:Immunoblotting / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; C083961:hemolysin BL protein, Bacillus