Coding theory based models for protein translation initiation in prokaryotic organisms.
Our research explores the feasibility of using communication theory, error control (EC) coding theory specifically, for quantitatively modeling the protein translation initiation mechanism. The messenger RNA (mRNA) of Escherichia coli K-12 is modeled as a noisy (errored), encoded signal and the ribosome as a minimum Hamming distance decoder, where the 16S ribosomal RNA (rRNA) serves as a template for generating a set of valid codewords (the codebook). We tested the E. coli based coding models on 5' untranslated leader sequences of prokaryotic organisms of varying taxonomical relation to E. coli including: Salmonella typhimurium LT2, Bacillus subtilis, and Staphylococcus aureus Mu50. The model identified regions on the 5' untranslated leader where the minimum Hamming distance values of translated mRNA sub-sequences and non-translated genomic sequences differ the most. These regions correspond to the Shine-Dalgarno domain and the non-random domain. Applying the EC coding-based models to B. subtilis, and S. aureus Mu50 yielded results similar to those for E. coli K-12. Contrary to our expectations, the behavior of S. typhimurium LT2, the more taxonomically related to E. coli, resembled that of the non-translated sequence group.
May
Elebeoba E
EE
Computational Biology Department, Sandia National Laboratories, Albuquerque, NM 87185, USA. eemay@sandia.vgov
Vouk
Mladen A
MA
Bitzer
Donald L
DL
Rosnick
David I
DI
D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics*; D016680:Genome, Bacterial; D016247:Information Storage and Retrieval / Q000379:methods*; D007257:Information Theory; D008957:Models, Genetic; D016366:Open Reading Frames / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
D001426:Bacterial Proteins