[Clinical and diagnostic importance of the evaluation of the Ig proteases activity in children with intestinal dysbacteriosis].
The specific activity of serine, metal dependent and thiolic Ig proteases in the coprofiltrates of children with manifestations of intestinal dysbacteriosis was determined by the enzyme immunoassay. 56 children with pronounced symptoms of intestinal disorders (37 children aged up to 1 year and 19 children over 1 year) were examined. A group of 25 clinically healthy children was used as control. Simultaneously with protease activity of coprofiltrates, there was detected the level of Ig-degrading activity of the opportunistic bacteria islolates of different taxonomic groups from feces of children with dysbacteriosis of different severity (as determined by the classical bacteriological method). The evaluation of the Ig-proteolytic activity of fecal supernatants, associated with the presence of serine, metal-dependent and thiolic proteases in the intestine, as well as detection of such proteases in microbial isolates, seems to be highly important for the diagnosis of intestinal disorders in children and is recommended for screening of intestinal dysbacteriosis.
Zinkevich
O D
OD
Bondarenko
V M
VM
Tiurin
Iu A
IuA
Safina
N A
NA
Anokhin
V A
VA
D001424:Bacterial Infections / Q000175:diagnosis / Q000201:enzymology*; D003248:Constipation / Q000473:pathology; D004760:Enterocolitis / Q000473:pathology; D005243:Feces / Q000201:enzymology*; D005374:Filtration; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000378:metabolism; D007223:Infant; D007410:Intestinal Diseases / Q000175:diagnosis / Q000201:enzymology*; D007787:Lactose Intolerance / Q000473:pathology; D008670:Metals; D010146:Pain / Q000473:pathology; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013438:Sulfhydryl Compounds
D007074:Immunoglobulin G; D008670:Metals; D013438:Sulfhydryl Compounds; D012694:Serine; D010447:Peptide Hydrolases