title: Osmotic stress in barley regulates expression of a different set of genes than salt stress does.
abstract: Under high salt conditions, plant growth is severely inhibited due to both osmotic and ionic stresses. In an effort to dissect genes and pathways that respond to changes in osmotic potential under salt stress, the expression patterns were compared of 460 non-redundant salt-responsive genes in barley during the initial phase under osmotic versus salt stress using cDNA microarrays with northern blot and real-time RT-PCR analyses. Out of 52 genes that were differentially expressed under osmotic stress, 11, such as the up-regulated genes for pyrroline-5-carboxylate synthetase, betaine aldehyde dehydrogenase 2, plasma membrane protein 3, and the down-regulated genes for water channel 2, heat shock protein 70, and phospholipase C, were regulated in a virtually identical manner under salt stress. These genes were involved in a wide range of metabolic and signalling pathways suggesting that, during the initial phase under salt stress, several of the cellular responses are mediated by changes in osmotic potential.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ueda
authors.forename: Arumugam
authors.initials: A
authors.lastname: Kathiresan
authors.forename: Mayumi
authors.initials: M
authors.lastname: Inada
authors.forename: Yukio
authors.initials: Y
authors.lastname: Narita
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nakamura
authors.forename: Weiming
authors.initials: W
authors.lastname: Shi
authors.forename: Tetsuko
authors.initials: T
authors.lastname: Takabe
authors.forename: John
authors.initials: J
authors.lastname: Bennett
mesh_terms: D004798:Enzymes / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D004798:Enzymes; D012965:Sodium Chloride