title: Differential distribution of KChIPs mRNAs in adult mouse brain.
abstract: The K(+) channel interacting proteins (KChIPs1-4) interact with and modulate activity and trafficking of Kv4 potassium channels. We report here the distribution of KChIPs in adult mouse brain. KChIP1 was expressed in a subpopulation of neurons widely distributed in brain and enriched in Purkinje cells of the cerebellum and in the reticular thalamic and medial habenular nuclei. KChIP2 transcripts were highly expressed in layer IV of the cerebral cortex and in striatum and hippocampus, but expressed at low levels in cerebellum. KChIP3 transcripts were detected primarily in the layer V and deep layer VI of the cerebral cortex, the hippocampus, and the entire cerebellum. KChIP4 was highly expressed by neurons in layers II-IV of cortex and in hippocampus, thalamus and the Purkinje cell layer of the cerebellum. Collectively, different KChIPs appear to be expressed by selected neuronal populations in different brain regions where expression of Kv4.2 and Kv4.3 have been reported. These findings support the likelihood of functional interactions between KChIPs and Kv4 K(+) channels in brain.
authors.affiliation: The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.forename: Imre
authors.initials: I
authors.lastname: Kovacs
authors.forename: Zhuohua
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D051605:Kv Channel-Interacting Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D051672:Shal Potassium Channels; D014162:Transfection / Q000379:methods
chemical_list: D002135:Calcium-Binding Proteins; C496756:Csen protein, mouse; C497018:KCND2 protein, human; C496750:KCNIP1 protein, human; C496752:KCNIP2 protein, human; C496749:KCNIP3 protein, human; C496754:Kcnip1 protein, mouse; C496755:Kcnip2 protein, mouse; C455273:Kcnip4 protein, mouse; D051605:Kv Channel-Interacting Proteins; D015221:Potassium Channels; D012333:RNA, Messenger; D012097:Repressor Proteins; D051672:Shal Potassium Channels