Mitochondrial effects of HIV infection on the peripheral blood mononuclear cells of HIV-infected patients who were never treated with antiretrovirals.
To investigate the effects of HIV infection on mitochondrial DNA (mtDNA) content and other mitochondrial parameters, we used peripheral blood mononuclear cells (PBMCs) from 25 asymptomatic antiretroviral-naive human immunodeficiency virus (HIV)-infected patients and from 25 healthy control subjects. HIV-infected patients had significant decreases in mtDNA content (decrease, 23%; P<.05) and in the activities of mitochondrial respiratory chain (MRC) complex II (decrease, 41%; P<.001), MRC complex III (decrease, 38%; P<.001), MRC complex IV (decrease, 19%; P=.001), and glycerol-3-phosphate dehydrogenase (decrease, 22%; P<.001), along with increased lipid peroxidation of PBMC membranes (P=.007). Therefore, HIV infection is associated not only with mtDNA depletion, but also with extensive MRC disturbances and increased oxidative damage.
Miró
Oscar
O
Mitochondrial Research Laboratory, Muscle Research Unit, Department of Internal Medicine, Hospital Clínic, Villarroel 170, 08036 Barcelona, Catalonia, Spain. omiro@clinic.ub.es
López
Sònia
S
Martínez
Esteban
E
Pedrol
Enric
E
Milinkovic
Ana
A
Deig
Elisabeth
E
Garrabou
Glòria
G
Casademont
Jordi
J
Gatell
Josep M
JM
Cardellach
Francesc
F
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004272:DNA, Mitochondrial / Q000378:metabolism; D005260:Female; D015658:HIV Infections / Q000473:pathology*; D006679:HIV Seropositivity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000821:virology*; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000821:virology*; D019562:Viral Load
D004272:DNA, Mitochondrial