title: Bone marrow origin of cells with capacity for homing and differentiation to esophageal squamous epithelium.
abstract: Our goal was to determine whether esophageal progenitor cells could be isolated from adult mouse esophagus or bone marrow and shown to home to and proliferate in the irradiated esophagus of recipient mice. Esophageal progenitor cells were isolated from adult male C3H/HeNsd or C57BL/6J green fluorescent protein (GFP(+)) mice by a serial in vitro preplate technique or the technique of side population cell sorting. When injected intravenously (i.v.), these cells homed to the 30-Gy-irradiated esophagus of GFP(-) female recipient mice and formed donor-origin esophageal foci. GFP(+) whole murine bone marrow cells injected i.v. also formed donor-origin esophageal squamous cell foci and protected recipient GFP(-) mice from upper-body irradiation in a cell dose-dependent manner. Marrow chimeric GFP(-) mice reconstituted with GFP(+) cells showed migration of GFP(+) marrow cells to the esophagus after 30 Gy irradiation. Purified esophageal progenitor cells isolated from first-generation preplate cell recipients engrafted after i.v. injection to the esophagus of second-generation-irradiated recipient mice. These data establish that esophageal progenitor cells can home to the irradiated esophagus and show limited differentiation capacity to squamous epithelium.
authors.affiliation: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Epperly
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guo
authors.forename: Hongmei
authors.initials: H
authors.lastname: Shen
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Niu
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
authors.forename: Mia
authors.initials: M
authors.lastname: Jefferson
authors.forename: Christine A
authors.initials: CA
authors.lastname: Sikora
authors.forename: Joel S
authors.initials: JS
authors.lastname: Greenberger
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D004847:Epithelial Cells / Q000166:cytology* / Q000528:radiation effects; D004947:Esophagus / Q000166:cytology* / Q000528:radiation effects*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL