[Significance of detecting HBV-DNA by the fluorescence quantitative PCR].
BACKGROUND
To study the correlativity between HBV-DNA and the markers of hepatitis B virus infection and different clinical types of hepatitis B.


METHODS
The fluorescence quantitation (FQ) of HBV-DNA of 105 patients with hepatitis B was performed by PCR, and the correlativity between the fluorescence quantitation of HBV-DNA and the markers of hepatitis B virus and different clinical types of hepatitis B was analyzed.


RESULTS
Ninety-seven percent of the patients were found HBsAg(+), HBeAg(+), HBcAb(+); 75% were HBsAg(+), HBeAb(+), HBcAb(+); 60% were HBsAg(+), HBcAb(+); 40% were HBsAg(+); in HBsAb(+), HBeAb(+), HBcAb(+) (or both HBsAb and HBcAb were positive) group the HBV DNA was undetectable. The analysis indicated that there was a significant difference among different groups (P less than 0.05).HBV-DNA was detected in 72.2% in acute hepatitis B group, in 75% of chronic hepatitis B group, and in 70% of cases of liver cirrhosis with hepatitis B group. The analysis indicated that there was no significant difference among the different clinical types of hepatitis (P greater than 0.05).


CONCLUSIONS
The levels of viral replication were not correlated with different clinical types of hepatitis B; the concentration of HBV-DNA in serum was related to hepatitis B antigen.
Wan
Bao-mei
BM
Department of Infectious Diseases, The Affiliated Hospital of Taishan Medical College, Shandong Taian 271000, China.
Liu
Shu-jun
SJ
Liu
Xin-jun
XJ
Yin
Yan-ming
YM
Li
He-lou
HL
Tan
Yong-xing
YX
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005453:Fluorescence; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D014779:Virus Replication
D004279:DNA, Viral