Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility.
We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.
Lightbody
Kirsty L
KL
Department of Biochemistry, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK.
Renshaw
Philip S
PS
Collins
Michelle L
ML
Wright
Rebecca L
RL
Hunt
Debbie M
DM
Gordon
Stephen V
SV
Hewinson
R Glyn
RG
Buxton
Roger S
RS
Williamson
Richard A
RA
Carr
Mark D
MD
D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D046912:Multiprotein Complexes; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000032:analysis
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D046912:Multiprotein Complexes; D001616:beta-Galactosidase