title: An epidemic of caprine arthritis encephalitis in Japan: isolation of the virus.
abstract: A disease characterized by arthritis of carpal joints and occasionally by pneumonia was seen among goats on a farm in Nagano prefecture of Japan in the summer of 2002. Serological investigation was done on 30 goats, that is one group on the farm by agar gel immunodiffusion tests using maedi-visna virus as the antigen, and 19 goats were positive. The caprine arthritis encephalitis virus genome was detected in peripheral blood leukocytes from several antibody-positive goats. Two goats among them were necropsied and used for pathological tests and virus isolation. The isolated virus was serially propagated in fetal lamb lung cell cultures. Goats inoculated with the culture fluid became antibody positive. These results suggested that a CAEV-infected goat had been introduced accidentally to the farm and that subclinical infection occurred among the flock.
authors.affiliation: National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Konishi
authors.forename: Satoko
authors.initials: S
authors.lastname: Tsuduku
authors.forename: Makoto
authors.initials: M
authors.lastname: Haritani
authors.forename: Kenji
authors.initials: K
authors.lastname: Murakami
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Chiho
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Kumiko M
authors.initials: KM
authors.lastname: Kimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured / Q000648:ultrastructure; D017931:DNA Primers; D004196:Disease Outbreaks; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D006041:Goats; D005779:Immunodiffusion; D007564:Japan / Q000453:epidemiology; D016180:Lentivirus Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D017931:DNA Primers