Identification of antigen-specific IgG in sera from patients with chronic prostatitis.
Antigen-specific vaccines are one of several molecularly targeted approaches under investigation as possible treatments for prostate cancer. Important to the development of vaccines is the identification of appropriate target antigens. We hypothesized that antigens of the prostate might be identified in patients with the chronic prostatitis/pelvic pain syndrome, a syndrome for which an autoimmune pathology has been proposed. Such antigens might represent naturally recognized target antigens of the prostate that could be investigated in the future as prostate tumor antigens. In this report, we used SEREX to identify proteins expressed in a prostate cDNA expression library recognized by IgG from the sera of patients with chronic prostatitis. Candidate proteins were evaluated using a panel of sera from 62 subjects with symptomatic prostatitis and 71 control male blood donors. We identified one protein that was recognized primarily in sera from subjects with prostatitis compared with controls. MAD-PRO-34, a nucleolar autoantigen, was recognized in 6/62 subjects and 0/71 controls (p = 0.00897). This protein had previously been identified as an autoantigen in patients with prostate cancer. In addition, the NY-CO-7 protein was recognized in 9/62 subjects and 3/71 controls (p = 0.0654). Two subjects had IgG specific for both the MAD-PRO-34 and NY-CO-7 gene products. Our results demonstrate that some patients with the chronic prostatitis/pelvic pain syndrome have autoantibodies to specific proteins. Proteins identified, and MAD-PRO-34 in particular, could be further investigated as potential prostate tumor antigens.
Dunphy
Edward J
EJ
Department of Medicine, Section of Medical Oncology, University of Wisconsin, Madison, Wisconsin 53792, USA.
Eickhoff
Jens C
JC
Muller
Charles H
CH
Berger
Richard E
RE
McNeel
Douglas G
DG
D000941:Antigens / Q000276:immunology*; D002908:Chronic Disease; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D011472:Prostatitis / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D012698:Serologic Tests; D044967:Serum / Q000276:immunology*
D000941:Antigens; D007074:Immunoglobulin G; D012333:RNA, Messenger