A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway.
Arabidopsis thaliana RD26 cDNA, isolated from dehydrated plants, encodes a NAC protein. Expression of the RD26 gene was induced not only by drought but also by abscisic acid (ABA) and high salinity. The RD26 protein is localized in the nucleus and its C terminal has transcriptional activity. Transgenic plants overexpressing RD26 were highly sensitive to ABA, while RD26-repressed plants were insensitive. The results of microarray analysis showed that ABA- and stress-inducible genes are upregulated in the RD26-overexpressed plants and repressed in the RD26-repressed plants. Furthermore, RD26 activated a promoter of its target gene in Arabidopsis protoplasts. These results indicate that RD26 functions as a transcriptional activator in ABA-inducible gene expression under abiotic stress in plants.
Fujita
Miki
M
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Fujita
Yasunari
Y
Maruyama
Kyonoshin
K
Seki
Motoaki
M
Hiratsu
Keiichiro
K
Ohme-Takagi
Masaru
M
Tran
Lam-Son Phan
LS
Yamaguchi-Shinozaki
Kazuko
K
Shinozaki
Kazuo
K
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D003890:Desiccation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000502:physiology; D011523:Protoplasts / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
D029681:Arabidopsis Proteins; C493887:RD26 protein, Arabidopsis; D018749:RNA, Plant; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D000040:Abscisic Acid