title: Enzyme assays for high-throughput screening.
abstract: Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic activity is detected using labelled substrates or indirect sensor systems that produce a detectable spectroscopic signal upon reaction. Recent advances in the development of high-throughput enzyme assays have identified new labels and chromophores to detect a wide range of enzymes activities. Enzyme activity profiling and fingerprinting have also been used as tools for identification and classification, while microarray formats have been devised to increase throughput.
authors.affiliation: Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Goddard
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Reymond
mesh_terms: D002384:Catalysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004798:Enzymes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004798:Enzymes