[Morphological changes following airway remodeling in asthmatic rats].
OBJECTIVE
To explore the establishment of an asthmatic model with airway remodeling in rats by observing the morphological changes of the airway in different stages.


METHODS
Animals were divided into two groups: asthmatic group and control group. The subjects were observed at 5 phases: before activation and 2, 4, 6, 8 weeks after activation. General histological changes were observed using hematoxylin and eosin stained sections. Being standardized by internal perimeter (Pi), the wall thickness (d), internal area (Ai), outer area (Ao) and wall area (WA) of the airway were quantified by computer-assisted image analysis system. And the membrane airways were divided into 3 grades (large, media and small airways) to be analyzed.


RESULTS
The changes of small airway appeared earlier and greater than the others. The changes of media airway appeared later than the small one. The changes of large airway appeared as later as the 4th week after activation. There were no differences before and after activation in control group (P>0.05).


CONCLUSIONS
(1) Prolonged, repeated and low density Ag activation could establish asthmatic model with airway remodeling. (2) The morphological changes of the airways increased following airway remodeling, and the greatest increase occurred at small airway. (3)The morphological changes in the model rats were similar to that in human beings.
Wang
Li-Xin
LX
Respiratory Department, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
Wu
Yin-Gen
YG
D056151:Airway Remodeling; D000818:Animals; D001249:Asthma / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012137:Respiratory System / Q000473:pathology*