title: Comparative immunogenicity analysis of modified vaccinia Ankara vectors expressing native or modified forms of hepatitis C virus E1 and E2 glycoproteins.
abstract: We have evaluated in C57/Bl6 and HLA-A2.1 transgenic mice the immunogenicity of three MVA vectors expressing either native HCV E1E2 polyprotein, truncated and secreted E1 (E'1(311)) and E2 (E'2(661)) proteins, or a chimeric E1E2 heterodimer presented at the plasma membrane. Immunization induced mainly a Th1 response in HLA-A2.1 transgenic mice while a Th2-type response was detected in C57/Bl6 mice. Comparison of the three vectors shows an increase in the humoral response when antigens are secreted or membrane bound, and slightly in the cellular response when antigens are exposed on the cell surface.
authors.affiliation: INSERM U544-Institut de Virologie, 67000 Strasbourg, France.
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Abraham
authors.forename: Nourredine
authors.initials: N
authors.lastname: Himoudi
authors.forename: François
authors.initials: F
authors.lastname: Kien
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Berland
authors.forename: Audrey
authors.initials: A
authors.lastname: Codran
authors.forename: Birke
authors.initials: B
authors.lastname: Bartosch
authors.forename: Thomas
authors.initials: T
authors.lastname: Baumert
authors.forename: Glaucia
authors.initials: G
authors.lastname: Paranhos-Baccala
authors.forename: Catherine
authors.initials: C
authors.lastname: Schuster
authors.forename: Geneviève
authors.initials: G
authors.lastname: Inchauspé
authors.forename: Marie Paule
authors.initials: MP
authors.lastname: Kieny
mesh_terms: D000818:Animals; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
chemical_list: C087035:E1 protein, Hepatitis C virus; D018937:Hepatitis C Antibodies; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines; D007371:Interferon-gamma