title: Inhibitory effects of extracts from peels of Citrus natsudaidai encapsulated in hybrid liposomes on the growth of tumor cells in vitro.
abstract: Inhibitory effects of extracts from peels of Citrus natsudaidai (natsumikan) encapsulated in hybrid liposomes (HL) composed of L-alpha-dimyristoylphosphatidylcholine and polyoxyethylene (20) sorbitan monolaurate on the growth of tumor cells were examined. The extracts with lower polar solvents inhibited the growth of B-16 mouse melanoma and human lung carcinoma cells, although the extracts with higher polar solvents showed no antitumor activity. In particular, the inhibitory effects of extracts with lower polar solvents encapsulated in HL were enhanced as compared with those of free extracts. Fluorescence microscopic analysis indicated that the HL including petroleum ether extracts induced apoptosis in B-16 mouse melanoma cells. On the other hand, the viability of normal human fibroblast cells was even less affected by the extracts of natsumikan. These results suggest that hydrophobic antitumor agents should be present in peels of natsumikan.
authors.affiliation: Division of Applied Chemistry, Graduate School of Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kadota
authors.forename: Chiho
authors.initials: C
authors.lastname: Taniguchi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Masuhara
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Shintaro
authors.initials: S
authors.lastname: Furusaki
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Iwahara
authors.forename: Koichi
authors.initials: K
authors.lastname: Goto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ueoka
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D008081:Liposomes; D008545:Melanoma; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013274:Stomach Neoplasms
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D010936:Plant Extracts