[Effect of Sophoricoside on histomorphology of bone in ovariectomized rats].
OBJECTIVE
To evaluate the potential preventive effect of Sophoricoside on bone loss in ovariectomized rats.


METHODS
Female SD rats (n=50, 6 months old) were either sham-operated (SHAM group, n=10) or ovariectomized (n=40). Three days after operation, ovariectomized rats were randomly assigned to groups as follows: 10 received Sophoricoside 4 mg.kg(-1).d(-1) (SL group), 10 received Sophoricoside 8 mg.kg(-1).d(-1) (SM group), 10 received Sophoricoside 16 mg.kg(-1).d(-1) (SH group) and 10 were untreated (OVX group). After Sophoricoside were given orally for one month, the histomorphometric parameters in the secondary spongiosa of proximal tibia and lumbar vertebrae were examined.


RESULTS
Compared with OVX group, SM and SH two kinds of treatment caused 15.28% and 22.81% increment in percent trabecular area (BV/TV, %) in the secondary spongiosa of proximal tibia, 14.23% and 21.2% increment in lumbar vertebrae. Accordingly in these two groups, there was a significant decrease in trabecular separation (FLAW, microm), but increment in trabecular width (Tb. Th, microm) and conjunction points (JOINT). But in SL group, the preventive effect was not observed.


CONCLUSIONS
Sophoricoside can be efficient in preventing ovariectomy-induced bone loss in rats.
Du
Ning
N
Department of Orthopaedics and Traumatology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. duning@vip.163.com
Xu
Yong
Y
Chen
Wei-Zhen
WZ
Zhang
Feng-Hua
FH
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D015519:Bone Density / Q000187:drug effects*; D005260:Female; D009929:Organ Size; D010024:Osteoporosis / Q000188:drug therapy / Q000517:prevention & control*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*
D001578:Benzopyrans; D012503:Saponins; C120642:sophoricoside