Arrays for the combinatorial exploration of cell adhesion.
A new method for the fabrication of arrays of self-assembled monolayers (SAMs) of alkane thiols (ATs) on gold to combinatorially assay surfaces for cell adhesion is reported. A fluorous SAM, which is both cytophobic and solvophobic, was used as the background between the array features. The resulting solvophobic background permits the application of an assembly after conjugation strategy for fabrication. SAMs containing mixtures of ATs and peptide-terminated ATs were generated. Multiple cell types demonstrated differential and specific binding to these surfaces. Additionally, pluripotent human embryonic stem cells proliferated on surfaces generated by this method.
Orner
Brendan P
BP
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
Derda
Ratmir
R
Lewis
Rachel L
RL
Thomson
James A
JA
Kiessling
Laura L
LL
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D041701:Swiss 3T3 Cells
D009842:Oligopeptides; D010455:Peptides; D013438:Sulfhydryl Compounds; C061514:glycyl-tyrosyl-isoleucyl-glycyl-seryl-arginine; D011092:Polyethylene Glycols; D006046:Gold; C047981:arginyl-glycyl-aspartic acid