'Signature sets', minimal fragment sets for identifying protein disulfide structures with cyanylation-based mass mapping methodology.
Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods can overcome the failure to detect fragments by demonstrating the existence of small 'signature sets' of fragments. The link between signature sets and the robustness of CN-based methodology is validated by two case studies.
Wu
Wei
W
Departments of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
Huang
Wei
W
Qi
Jianfeng
J
Chou
Yi-Te
YT
Torng
Eric
E
Watson
J Throck
JT
D003486:Cyanides / Q000737:chemistry*; D003553:Cystine / Q000032:analysis* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
D003486:Cyanides; D004220:Disulfides; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D003553:Cystine