title: [Capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum].
abstract: BACKGROUND
  To develop a capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.
  
  
  METHODS
  The anti-HCMV-IgM antibody was detected in 68 patients with HCMV infection by the capturing-ELISA, and the results were compared with those of indirect ELISA.
  
  
  RESULTS
  The specificity and sensitivity of the capturing-ELISA were shown to be significantly higher than those of indirect ELISA, and its results were not affected by RF factor.
  
  
  CONCLUSIONS
  The capturing ELISA is specific, sensitive, convenient and reliable method which may be feasible for clinical use.
authors.affiliation: Laboratory of Medical Technology, Hangzhou Sixth People's Hospital, Zhejiang Province, Hangzhou 310014, China.
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Pei
authors.initials: P
authors.lastname: Li
authors.forename: Hong-jing
authors.initials: HJ
authors.lastname: Liu
authors.forename: Bao-gen
authors.initials: BG
authors.lastname: Liu
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M