title: Expression, purification and preliminary crystallographic studies of human coactosin-like protein.
abstract: Coactosin-like protein (CLP) is an actin-binding protein as well as a 5-lipoxygenase binding partner. Human coactosin-like protein has been expressed in high yield and the His-tagged protein was purified by affinity chromatography. Several different crystal forms were obtained by the hanging-drop vapour-diffusion method. X-ray diffraction data to 2.0 A resolution were collected from the best crystal. The space group was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 38.4, b = 48.7, c = 72.6 A.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Cheng
authors.forename: Mao
authors.initials: M
authors.lastname: Wan
authors.forename: Zhaocai
authors.initials: Z
authors.lastname: Zhou
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
mesh_terms: D001923:Brain Chemistry; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003460:Crystallization; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003627:Data Interpretation, Statistical; D006801:Humans; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014961:X-Ray Diffraction
chemical_list: C118115:COTL1 protein, human; D018076:DNA, Complementary; D008840:Microfilament Proteins