title: Characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea.
abstract: Genetic and antigenic characterizations of 70 strains of adenovirus type 41 (Ad41), isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea, were done by DNA restriction enzyme (RE) analysis, sequencing analysis, and monoclonal antibody (MAb)-based enzyme-linked immunosorbent assay (ELISA). Eight genome types were observed in the present study, among which D25, D26, D27, and D28 were novel genome types. These eight genome types were divided into two genome-type clusters (GTCs) based on phylogenetic analysis of the hypervariable regions (HVRs) of the hexon. GTC1 includes D1, D25, D26, D27, and D28, and the GTC2 contains D4, D12, and D22. The amino acid homologies among the members within a GTC were 97 to 100%, whereas between the members of different GTCs the homologies were 92 to 94%. The specificity of the GTC classification was confirmed by ELISA with MAb 1F, which was selected by the Ad41 prototype Tak strain. It was found that only the isolates of GTC1 but not of GTC2 reacted with MAb 1F. These results suggest that Ad41 isolates from the three countries should be classified into two subtypes. The accumulation of amino acid mutations located in HVRs of hexon are indicative for the classification of Ad41 subtype.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-0033, Japan.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Lan Thi Phuong
authors.initials: LT
authors.lastname: Doan
authors.forename: Phan Gia
authors.initials: PG
authors.lastname: Tung
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishio
authors.forename: Eiko
authors.initials: E
authors.lastname: Suzuki
authors.forename: Jeong Kee
authors.initials: JK
authors.lastname: Seo
authors.forename: Kyo Sun
authors.initials: KS
authors.lastname: Kim
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000208:Acute Disease; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005759:Gastroenteritis / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D007564:Japan; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014744:Vietnam
chemical_list: D017931:DNA Primers