The influence of NaCl and carbonylcyanide-m-chlorophenylhydrazone on the production of extracellular proteases in a marine Vibrio strain.
In general, the salinity of the ocean is close to 3.5% and marine vibrios possess the respiratory chain-linked Na+ pump. The influence of sodium chloride and the proton conductor carbonylcyanide m-chlorophenylhydrazone (CCCP) on the production of extracellular proteases in a marine Vibrio strain was examined. At the concentration of 0.5 M, sodium chloride minimally inhibited the activity of extracellular proteases by approximately 16%, whereas at the same concentration, the producton of extracellular proteases was severely inhibited. On the other hand, the production of extracellular proteases was completely inhibited by the addition of 2 microM CCCP at pH 8.5, where the respiratory chain-linked Na+ pump functions.
Kim
Young Jae
YJ
Department of Microbiology, Changwon National University, Sarim-dong, Changwon, Kyungnam 641-773, Republic of Korea. yjkim@changwon.ac.kr
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D021381:Protein Transport; D012965:Sodium Chloride / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase; D014475:Uncoupling Agents / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects / Q000201:enzymology*
D014475:Uncoupling Agents; D012965:Sodium Chloride; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D010450:Endopeptidases; D000254:Sodium-Potassium-Exchanging ATPase