The human Duffy antigen binds selected inflammatory but not homeostatic chemokines.
The aim of the study was to compare the ability of the human Duffy antigen to bind homeostatic and inflammatory chemokines. Homeostatic chemokines did not bind to the Duffy antigen on erythrocytes with high affinity. In contrast, 60% of inflammatory chemokines bound strongly to Duffy, with no obvious preference for CXC or CC classes. It was investigated if this binding profile was reflected in the binding pattern of endothelial cells. Two examples of homeostatic (125I-CXCL12 and 125I-CCL21) and inflammatory (125I-CXCL8 and 125I-CCL5) chemokines were incubated with human synovia. In agreement with the erythrocyte binding data, intense specific signals for CXCL8 and CCL5 binding were found on endothelial cells, whereas CXCL12 and CCL21 showed only weak binding to these cells. Our study provides evidence that the human Duffy antigen binds selected inflammatory, but not homeostatic, chemokines and that this binding pattern is reflected by endothelial cells within inflamed and non-inflamed tissue.
Gardner
Lucy
L
Leopold Muller Arthritis Research Centre, Institute for Science and Technology in Medicine, Medical School, Keele University at Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire SY10 7AG, UK.
Patterson
Angela M
AM
Ashton
Brian A
BA
Stone
Michael A
MA
Middleton
Jim
J
D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D001667:Binding, Competitive; D018925:Chemokines / Q000378:metabolism*; D004375:Duffy Blood-Group System / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D011485:Protein Binding
D018925:Chemokines; D004375:Duffy Blood-Group System; D018836:Inflammation Mediators