Forefront of Na+/Ca2+ exchanger studies: physiology and molecular biology of monovalent cation sensitivities in Na+/Ca2+ exchangers.
Sensitivities of the reverse-mode Na+/Ca2+ exchange activity measured as the Na+i-dependent Ca2+ uptake to extracellular monovalent cations K+, Li+, and Na+ were compared between the K+ -dependent (NCKX2) and the K+ -independent Na+/Ca2+ exchanger (NCX1) overexpressed in a fibroblast cell. Interestingly, the exchange activity of NCKX2 was not influenced by Li+ while it was increased by K+. On the contrary, the activity of NCX1 was increased by Li+. Thus, the cation sensitivities to K+ and Li+ markedly differed between NCKX2 and NCX1. In addition, Na+ exerted a significantly smaller inhibitory effect on the activity in NCKX2 than in NCX1. The Na+/Ca2+ exchange activities of NCKX2 and NCX1 are considered to be regulated differentially via the respective binding site domains that have distinct sensitivities to the external monovalent cations.
Uehara
Akira
A
Department of Physiology, School of Medicine, Fukuoka University, 7-chome Nanakuma, Jonan-ku, Japan. Ueharaak@fukuoka-u.ac.jp
Iwamoto
Takahiro
T
Nakamura
Yuki
Y
Imanaga
Issei
I
D000595:Amino Acid Sequence; D000818:Animals; D002414:Cations, Monovalent / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D019831:Sodium-Calcium Exchanger / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*
D002414:Cations, Monovalent; D019831:Sodium-Calcium Exchanger