Expression patterns of integrins on pleomorphic adenoma and adenoid cystic carcinoma: study on specimens and in vitro investigation of the effects of extracellular matrix on the expression of these adhesion molecules.
BACKGROUND
Pleomorphic adenoma (PA) and adenoid cystic carcinoma (ACC) are neoplasms of distinct behaviour, showing similar origin, cell components and marked presence of extracellular matrix (ECM). Interactions between cells and ECM are important in the biology of tumours, being partially mediated by integrins. This study investigated these interactions on PA and ACC using paraffin-embedded tissue and an in vitro model of these conditions.


METHODS
Expression of integrins in paraffin-embedded samples was assessed by immunohistochemistry. Cells from PA and ACC were characterized using immunofluorescence, and integrin patterns of expression were investigated on cells cultivated on different ECM proteins.


RESULTS
Luminal cells of both PA and ACC were more intensely positive for integrins than myoepithelial cells. In vitro studies revealed that PA cells expressed more integrins than ACC cells regardless the ECM protein present.


CONCLUSIONS
This study revealed particular patterns of integrin expression in both specimens and in vitro models of PA and ACC. This might prove useful for a better understanding of the biology of these lesions.
Lourenço
S V
SV
Instituto de Medicina Tropical de São Paulo and Department of Dermatology, Medical School of the University of São Paulo, São Paulo, SP, Brazil. sloducca@usp.br
Kapas
S
S
Williams
D M
DM
Leite
K
K
Araújo
V C
VC
D000199:Actins / Q000032:analysis; D008949:Adenoma, Pleomorphic / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D024042:Collagen Type I / Q000032:analysis; D024141:Collagen Type IV / Q000032:analysis; D005109:Extracellular Matrix / Q000473:pathology*; D005347:Fibroblasts / Q000473:pathology; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D039421:Integrin alpha2 / Q000032:analysis; D039422:Integrin alpha3 / Q000032:analysis; D039482:Integrin alpha5 / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D016023:Integrins / Q000032:analysis*; D007797:Laminin / Q000032:analysis; D008840:Microfilament Proteins; D017154:Stromal Cells / Q000473:pathology; D014746:Vimentin / Q000032:analysis; D000096985:Calponins
D000199:Actins; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D024042:Collagen Type I; D024141:Collagen Type IV; D005353:Fibronectins; D039421:Integrin alpha2; D039422:Integrin alpha3; D039482:Integrin alpha5; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D008840:Microfilament Proteins; D014746:Vimentin; D000096985:Calponins