Cytokine mRNA expression in B cells from bovine leukemia virus-infected cattle with persistent lymphocytosis.
We have characterized the expression of six cytokine mRNAs in highly purified B cells from bovine leukemia virus (BLV)-infected cows with persistent lymphocytosis. Selected cytokine mRNAs included those encoding tumor necrosis factor (TNF), lymphotoxin-alpha (LT-alpha), transforming growth factor-beta1 (TGF-beta1), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and interleukin-10 (IL-10). Fresh B cells from cows with persistent lymphocytosis constitutively transcribed TNF, LT-alpha and TGF-beta1 mRNAs. Although IL-1beta, IL-6 and IL-10 mRNAs were barely detectable in fresh B cells from cows with persistent lymphocytosis, transcripts encoding these cytokines were strongly and rapidly upregulated in B cells after cell culture. Results from this study provide the first evidence that B cells infected with BLV express specific cytokine mRNAs in vivo.
Amills
Marcel
M
Department of Animal Sciences, Laboratory of Immunogenetics, Edward R. Madigan Laboratory, 1201 West Gregory Drive, University of Illinois at Urbana-Champaign, Urbana 61801, USA. marcel.amills@uab.es
Norimine
Junzo
J
Olmstead
Colleen A
CA
Lewin
Harris A
HA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D007378:Interleukins / Q000235:genetics; D001909:Leukemia Virus, Bovine; D012333:RNA, Messenger / Q000235:genetics
D016207:Cytokines; D017931:DNA Primers; D007378:Interleukins; D012333:RNA, Messenger