Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential.
In vitro maturation (IVM) of goat oocytes with serum-supplemented media results in oocytes with reduced developmental potential. The objective of this study was to develop a defined medium for IVM of goat oocytes that better supports subsequent embryonic development. Cumulus oocyte complexes (COC) were matured for 18-20 hr in: Experiment (1), tissue culture medium 199 (TCM199) with 10% (v/v) goat serum or modified synthetic oviduct fluid maturation medium (mSOFmat) with 2.5, 8.0, or 20.0 mg/ml bovine serum albumin (BSA); Experiment (2), mSOFmat with 4.0, 8.0, 12.0, or 16.0 mg/ml BSA; or Experiment (3), 1.0 mg/ml polyvinyl alcohol (PVA; control), 4.0 mg/ml BSA, 0.5 mg/ml hyaluronate plus 0.5 mM citrate, or hyaluronate, citrate, and BSA. Mature COC were coincubated for 20-22 hr with 12-15 x 10(6) sperm/ml in modified Brackett and Oliphant (mBO) medium. Embryos were cultured for a total of 7 days in G1/2, and evaluated for cleavage, and blastocyst development, hatching, and total cell numbers. In the first experiment, more (P < 0.05) blastocysts developed per cleaved embryo following maturation in mSOFmat with 2.5 or 8.0 mg/ml BSA than with 20.0 mg/ml BSA or TCM199 with 10% goat serum. The various concentrations of BSA used in the second experiment did not affect (P > 0.05) any of the developmental endpoints examined. In the third experiment, developmental potential of oocytes matured with PVA or hyaluronate with citrate was not different (P > 0.05) from oocytes matured in the presence of BSA. These results demonstrate that developmentally competent goat oocytes can be matured under defined conditions.
Herrick
J R
JR
Department of Animal Sciences, Center for Comparative Medicine, Purdue University, West Lafayette, Indiana, USA.
Behboodi
E
E
Memili
E
E
Blash
S
S
Echelard
Y
Y
Krisher
R L
RL
D000818:Animals; D018929:Cell Culture Techniques; D019343:Citric Acid; D003470:Culture Media; D006041:Goats / Q000378:metabolism; D006820:Hyaluronic Acid; D009865:Oocytes / Q000378:metabolism*; D044967:Serum; D012710:Serum Albumin, Bovine
D003470:Culture Media; D012710:Serum Albumin, Bovine; D019343:Citric Acid; D006820:Hyaluronic Acid