A validated chiral HPLC method for the enantiomeric separation of tolterodine tartarate.
An isocratic chiral HPLC method was developed for the separation of tolterodine tartarate enantiomers. The mobile phase consists of n-hexane and isopropyl alcohol in the ratio of 980:20 (v/v) with 1 ml diethylamine and 0.6 ml trifluoroacetic acid. Chiralcel OD-H (250 mm x 4.6mm) column was used at constant room temperature. Flow rate was kept at 0.5 ml/min. This method is capable of detecting the S-isomer up to 0.1 microg/ml. The method was validated in terms of linearity, precision, limit of detection (LOD) and limit of quantification (LOQ).
Kumar
Y Ravindra
YR
Dr. Reddy's Laboratories Ltd., Bulk Actives Unit-III, IDA-Bollaram, Hyderabad 502325, AP, India.
Ramulu
G
G
Vevakanand
V V
VV
Vaidyanathan
Gopal
G
Srinivas
Keesari
K
Kumar
M Kishore
MK
Mukkanti
K
K
Reddy
M Satyanarayana
MS
Venkatraman
S
S
Suryanarayana
M V
MV
D001559:Benzhydryl Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000737:chemistry*; D015394:Molecular Structure; D010665:Phenylpropanolamine / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D000068737:Tolterodine Tartrate
D001559:Benzhydryl Compounds; D003408:Cresols; D010665:Phenylpropanolamine; D000068737:Tolterodine Tartrate