title: Human dendritic cells are less potent at killing Candida albicans than both monocytes and macrophages.
abstract: Dendritic cells (DC) function as professional phagocytes to kill Candida albicans and subsequently present it to the adaptive immune system. Monocytes, macrophages and DC were generated from five individual donors and their Candida-killing capacity and cytokine release were assessed. Compared to monocytes and macrophages, DC from healthy volunteers were significantly less effective in C. albicans--stimulated cytokine release, killing of C. albicans blastoconidia and damaging of C. albicans hyphae. In conclusion, while important as antigen-presenting cells and initiators of the adaptive immune system, DC are poor in both intracellular killing and damaging of C. albicans hyphae. Effective handling of large numbers of C. albicans is the prime task of the innate immune system consisting of large numbers of neutrophils and monocytes.
authors.affiliation: Department of Medicine, University Medical Center St. Radboud, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
authors.forename: Mihai G
authors.initials: MG
authors.lastname: Netea
authors.forename: Karlijn
authors.initials: K
authors.lastname: Gijzen
authors.forename: Neeltje
authors.initials: N
authors.lastname: Coolen
authors.forename: Ineke
authors.initials: I
authors.lastname: Verschueren
authors.forename: Carl
authors.initials: C
authors.lastname: Figdor
authors.forename: Jos W M
authors.initials: JW
authors.lastname: Van der Meer
authors.forename: Ruurd
authors.initials: R
authors.lastname: Torensma
authors.forename: Bart Jan
authors.initials: BJ
authors.lastname: Kullberg
mesh_terms: D002176:Candida albicans / Q000254:growth & development* / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000032:analysis; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D037181:Lectins, C-Type / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D007371:Interferon-gamma; D015850:Interleukin-6; D016209:Interleukin-8; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin