title: Solid-phase synthesis of circular oligonucleotides.
abstract: A protocol for the straightforward preparation of small circular oligodeoxyribonucleotides (2-28 nt) is reported. The assembly of the oligonucleotide chain (standard phosphoramidite chemistry) and cyclization by the phosphotriester method take place on a tailor-made nucleotide-derivatized solid support. Although cyclization yields are moderate, the procedure exploits a synthesis design that allows selective cleavage of the circular oligonucleotide from the support, which facilitates isolation of the target molecule by simple filtration.
authors.affiliation: Department de Química Orgánica, Universitat de Barcelona, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Pedroso
authors.forename: Nuria
authors.initials: N
authors.lastname: Escaja
authors.forename: Miriam
authors.initials: M
authors.lastname: Frieden
authors.forename: Anna
authors.initials: A
authors.lastname: Grandas
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000138:chemical synthesis*
chemical_list: D009841:Oligonucleotides