title: Identification of TPO receptors on central nervous system-a preliminary report.
abstract: To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.
authors.affiliation: Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong. yang1091@cuhk.edu.hk
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Xia
authors.forename: Karen
authors.initials: K
authors.lastname: Li
authors.forename: Nga-Hin
authors.initials: NH
authors.lastname: Pong
authors.forename: Ki-Wai
authors.initials: KW
authors.lastname: Chik
authors.forename: Chi-Kong
authors.initials: CK
authors.lastname: Li
authors.forename: Margaret H L
authors.initials: MH
authors.lastname: Ng
authors.forename: Ho-Keung
authors.initials: HK
authors.lastname: Ng
authors.forename: Kwok-Pui
authors.initials: KP
authors.lastname: Fung
authors.forename: Tai-Fai
authors.initials: TF
authors.lastname: Fok
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001923:Brain Chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis*; D009474:Neurons / Q000187:drug effects; D015513:Oncogene Proteins / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D018121:Receptors, Cytokine / Q000032:analysis*; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: C481270:Mpl protein, mouse; D009363:Neoplasm Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; D004921:Erythropoietin; C082218:MPL protein, human; D013926:Thrombopoietin