Grape seed extract prevents H(2)O(2)-induced chromosomal damage in human lymphoblastoid cells.
We examined the effects of grape seed extract (GSE) on chromosomal damage in two ways; induction on its own and prevention against treatment of reactive oxygen species (ROS). Chromosomal damage was evaluated by cytokinesis-block micronucleus assay (CBMN) in a human lymphoblastoid cell line, WIL2-NS cells. The GSE was composed of 89% proanthocyanidin with a degree of polymerization ranging from 2 to 15. GSE did not induce chromosomal damage in WIL2-NS cells at GSE concentrations up to 5 mg/l. In contrast, pretreatment with GSE dose-dependently prevented H(2)O(2)-induced chromosomal damage at an effective dose of 0.3 to 1 mg/l. A similar preventive effect of GSE was not detected in tert-butyl hydroperoxide-induced damage even at 5 mg/l. In a cell free system, GSE (<5 mg/l) directly scavenged H(2)O(2), but produced slight amounts of H(2)O(2) at higher concentrations (>50 mg/l). These results suggest that GSE is not genotoxic, but rather has an antigenotoxic effect against H(2)O(2) via direct scavenging action of H(2)O(2).
Sugisawa
Ayako
A
IAA National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
Inoue
Shuji
S
Umegaki
Keizo
K
D001402:B-Lymphocytes / Q000187:drug effects; D002460:Cell Line; D002877:Chromosomes, Human / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
D010936:Plant Extracts; D006861:Hydrogen Peroxide