Targeting of Bacillus anthracis interaction factors for human macrophages using two-dimensional gel electrophoresis.
Bacillus anthracis, a gram-positive, endospore-forming, aerobic rod-shaped bacterium, interacts with macrophages at various stages of the disease. Spore germination and the outgrowth of vegetative bacilli are crucial steps enabling the bacteria to proliferate actively and to synthesize the virulence factors leading to a massive septicemia. In this study, we performed a proteomic analysis and MALDI-TOF/MS were carried out to identify proteins using human macrophages infected with the spores of B. anthracis live-Sterne or inactivated-Sterne. We identified 21 proteins which are related to the infection of B. anthracis spores on human macrophages at the early stage events. These proteins function in processes such as cytoskeleton regulation, apoptosis, cell division, and protein degradation. Proteins such as PAK 2 revealed a relationship to apoptosis in human macrophages. These proteins play an important role in the macrophage survival and death on human macrophages with infected B. anthracis spores.
Seo
Gwi-Moon
GM
Division of Molecular and Life Sciences, Hanyang University, Ansan 426-791, Republic of Korea.
Kim
Seong-Joo
SJ
Kim
Ji-Chon
JC
Nam
Deok-Hwa
DH
Yoon
Moon-Young
MY
Koo
Bon-Sung
BS
Chai
Young Gyu
YG
D001408:Bacillus anthracis / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013171:Spores, Bacterial / Q000502:physiology
D011506:Proteins