title: Mutation in a Lordsdale norovirus epidemic strain as a potential indicator of transmission routes.
abstract: An increase in norovirus outbreaks was reported internationally during 2002 and 2003 and was also observed in Oxfordshire (United Kingdom) hospitals. To understand their epidemiological relationships, viruses from 22 outbreaks (15 from one hospital) were subjected to nucleotide sequencing. The 3'-terminal 3,255 nt or complete genomes were determined for 49 viruses. All outbreaks were caused by a genogroup II norovirus related to the Lordsdale virus (GII 4), common in healthcare settings. The norovirus mutation rate was sufficiently high that the 3,255-nucleotide sequences allowed separate and potentially connected outbreaks to be identified, since all outbreaks with identical sequences were temporally or geographically linked. The high mutation rate was further indicated by four mutations and three microheterogeneities in 3,255 nucleotides during 17 days of norovirus shedding by an immunocompromised patient. The data suggested that multiple virus introductions from the community, occasional transmission among wards, and one instance of ongoing environmental contamination had occurred. The accumulation, or lack, of mutations within an outbreak was also used to indicate the predominant transmission route. In an outbreak where person-to-person spread was thought to predominate, six mutations were detected throughout the genome, whereas one mutation was detected when point source infection was suspected. This norovirus epidemic strain differed from its closest previously described relative by 11.4 to 13.6% in the outer P2 domain of the capsid, which also had a single-amino-acid insertion. Alterations to the capsid structure compared to previous noroviruses may explain the increased number of outbreaks during 2002 and 2003.
authors.affiliation: Nuffield Department of Clinical Sciences, Oxford University, John Radcliffe Hospital, Oxford, United Kingdom, OX3 9DU. kate.dingle@ndcls.ox.ac.uk
authors.forename: Kate E
authors.initials: KE
authors.lastname: Dingle
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000635:transmission*; D017931:DNA Primers; D004196:Disease Outbreaks; D004739:England / Q000453:epidemiology; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers