Culture of haematopoietic cells in a 3-D bioreactor made of Al2O3 or apatite foam.
Foams of Al2O3 and apatite ceramics with interconnecting pores were produced using a new technique. The surfaces of the ceramics served as substrates for the culture of human peripheral and bone marrow derived stem cells. Up to 27 days the cells were kept in culture where they proliferated and developed into different morphologies consistent with bone marrow cell lines.
Schubert
H
H
Institut für Werkstoffwissenschaften und-technologien, Technische Universität Berlin, Englische Strasse 20, D 10587 Berlin, Germany.
Garrn
I
I
Berthold
A
A
Knauf
W U
WU
Reufi
B
B
Fietz
T
T
Gross
U M
UM
D000537:Aluminum Oxide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D002455:Cell Division; D048429:Cell Size; D017886:Durapatite / Q000737:chemistry*; D005240:Feasibility Studies; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
D001672:Biocompatible Materials; D017886:Durapatite; D000537:Aluminum Oxide