Quantification and stability of salbutamol in human urine.
A sensitive method for the quantification of free salbutamol in human urine is described. Sample clean up is performed using SPE on a mixed phase extraction column. Derivatisation is performed with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) and the extract is analysed by GC-MS. The method was found to be suitable for use in the doping field, where a cut-off limit of 1 microg salbutamol/mL urine is set by the International Olympic Committee (IOC) and approved by the World Anti-Doping Agency (WADA). Above that value a doping violation occurs. In addition, the stability of salbutamol in human urine has been evaluated.
Forsdahl
Guro
G
Doping Control Laboratory, ARC Seibersdorf research GmbH, A-2224 Seibersdorf, Austria.
Gmeiner
Günter
G
D000081:Acetamides; D000420:Albuterol / Q000652:urine*; D004300:Doping in Sports; D004355:Drug Stability; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D014297:Trimethylsilyl Compounds
D000081:Acetamides; D005463:Fluoroacetates; D014297:Trimethylsilyl Compounds; C086665:N-methyl-N-(trimethylsilyl)trifluoroacetamide; D000420:Albuterol