[Aberrant development of pollen in transgenic tobacco expressing bacterial iaaM gene driven by pollen- and tapetum-specific promoters].
Microsporogenesis offers an ideal model for studying gene expression, cell division and cell to cell communication during development. The role of auxin in pollen development was investigated in transgenic tobacco plants expressing the coding region of the iaaM gene from Pseudomonas syringae, under control of the promoters Lat-52 (pollen-specific) or TA-29 (tapetum-specific). IAA level in anther of transgenic plants increased significantly, and transgenic plants displayed morphological aberrations not solely attributable to pollen development(such as adventitious root formation on stems, epinastic leaf growth, delayed flowering). These results suggest that expression of Lat-52 and TA-29 are not strictly limited to anther. Anther shape was changed and the number of pollen grains per anther was reduced, but grains could be stained with aceto-carmine. Almost all flowering plants were fertile, although the number of flowers per inflorescence was reduced compared with the wild-type ones. These results suggest that auxin plays an important role in pollen development, and over-expressing auxin synthesis gene could result in aberrant development of pollen.
Ni
Di An
DA
Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032.
Yu
Xiao Hong
XH
Wang
Ling Jian
LJ
Xu
Zhi Hong
ZH
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D011058:Pollen / Q000235:genetics* / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D001426:Bacterial Proteins; C081170:iaaM protein, Agrobacterium