Malt sprout extract medium for cultivation of Lactobacillus plantarum protective cultures.
OBJECTIVE
The aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative Lactobacillus plantarum VTT E-79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E-79098 and the antimicrobial activity of the cell-free extracts.


METHODS
Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell-free extracts against Pantoea agglomerans VTT E-90396 and Fusarium avenaceum VTT D-80147 was assessed with automated turbidometry.


CONCLUSIONS
When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E-79098 and enhanced antimicrobial potential against the Gram-negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low-cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.
Laitila
A
A
VTT Biotechnology, Espoo, Finland. arja.laitila@vtt.fi
Saarela
M
M
Kirk
L
L
Siika-Aho
M
M
Haikara
A
A
Mattila-Sandholm
T
T
Virkajärvi
I
I
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000494:pharmacology; D002523:Edible Grain / Q000737:chemistry*; D005670:Fusarium / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D020636:Pantoea / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D010936:Plant Extracts