title: [Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues].
abstract: OBJECTIVE
  To investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).
  
  
  METHODS
  One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.
  
  
  RESULTS
  EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.
  
  
  CONCLUSIONS
  Detection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.
authors.affiliation: Department of Pathology, Shihezi University School of Medicine, Xinjiang 832002, China. patho-sh@mail.xj.cninfo.net
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Chang
authors.forename: Xin-xia
authors.initials: XX
authors.lastname: Li
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Pang
authors.forename: Hong-fen
authors.initials: HF
authors.lastname: Lu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Meng-hong
authors.initials: MH
authors.lastname: Sun
authors.forename: Da-ren
authors.initials: DR
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D000328:Adult; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000175:diagnosis / Q000378:metabolism*; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D016612:Paraffin Embedding; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000175:diagnosis / Q000378:metabolism; D012512:Sarcoma, Ewing / Q000175:diagnosis / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000175:diagnosis / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS; D014157:Transcription Factors