[Characterization of expression of the centrosomal protein, Cenexin, in rat spermatogenesis].
The centrosomal protein, Cenexin, is a molecular marker of mature centriole. To elucidate the variability and function of mature centriole in spermatogenesis, the high titer polyclonal antibody against rat cenexin was obtained by immunizing mice with recombinant cenexin which was made up in this study. The expression of cenexin in rat spermatogenesis was carried out by semi-quantitative RT-PCR, immunofluorescence and Western Blot. The results demonstrated that the level of Cenexin mRNA was higher in spermatogonia and spermatocytes, then decreased in following stages, while Cenexin protein was located on one centriole from spermatogonia to spermatids, showing mature centriole existed in these stages. Cenexin protein was localized to the basal body of the flagellum in elongated spermatids and the stained signal disappeared in the most of epidydimal sperms. These results suggested that the expression pattern of cenexin in rat spermatogenesis might be related to the initiation of the flagella formation.
Sun
Xiao Dong
XD
Department of Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005.
Yu
Zuo Ren
ZR
Ma
Jing
J
Ge
Ye Hua
YH
Li
Sai
S
Xue
She Pu
SP
Han
Dai Shu
DS
D000818:Animals; D015153:Blotting, Western; D002502:Centrioles / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013093:Spermatogonia / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism
D006360:Heat-Shock Proteins; C490105:Odf2 protein, rat