[Comparison of hepatitis B virus serotype and genotype among HBsAg positive hepatitis B patients in a northern and a southern city of China].
OBJECTIVE
To understand HBV serotypes and genotypes epidemiology in a northern city and a southern city in China.


METHODS
Using polymerase chain reaction (PCR) and direct sequencing of HBV DNA PCR products, the serotypes and genotypes of HBV in 530 from HBsAg positive samples. The enrolled patients were from Harbin, a northern city and Lianjiang, a southern city in China.


RESULTS
Comparison of the serotypes and genotypes of HBV between Harbin and Lianjiang showed that adrq+ was the most predominant hepatitis B virus serotype in both Harbin and Lianjiang (87.2% and 73.5%,respectively), adw2 was the next (12.0% and 25.7%, respectively); genotype C was the most frequent in Harbin and Lianjiang (87.8% and 73.2%, respectively), and genotype B was the next (12.2% and 26.1%, respectively) only 1 patient was infected by genotype D, and 1 patient was found to be co-infected by genotype B and C in Lianjiang.


CONCLUSIONS
The results suggest that the percentage of HBV serotypes and genotypes between Harbin and Lianjiang was significantly different (P less than 0.001), but the main HBV serotype and genotype of the two cities were similar.
Xu
Jun
J
Hepatology Institute, People's Hospital, Beijing University, Beijing 100044, China.
Wang
Qi-xin
QX
Fan
Chun-lei
CL
Jiang
Dong
D
Li
Ruo-bing
RB
Cong
Xu
X
Fei
Ran
R
Chen
Hong-song
HS
Wei
Lai
L
Wang
Yu
Y
D002681:China; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012703:Serotyping
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens