title: Effect of combined T- and B-cell depletion of allogeneic HLA-mismatched bone marrow graft on the magnitude and kinetics of Epstein-Barr virus load in the peripheral blood of bone marrow transplant recipients.
abstract: Recipients of T-cell-depleted bone marrow (BM) transplants (BMT) frequently develop Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) preceded by a rapid and prominent increase of EBV load in the peripheral blood. The level of this increase positively correlates with the incidence of PTLD. Using a semiquantitative PCR assay we compared the blood levels of EBV-DNA in patients transplanted with either T-cell or T- and B-cell-depleted human leukocyte antigen (HLA)-mismatched BM grafts. Combined T- and B-cell depletion correlated with significantly lower maximal levels of EBV load, which were reached with slower kinetics. These data indicate that B-cell depletion of BM can be used for prophylaxis of PTLD in BM transplant recipients and can affect the long-term balance between EBV and its host.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Daorong
authors.initials: D
authors.lastname: Liu
authors.forename: Charlotte
authors.initials: C
authors.lastname: Tammik
authors.forename: Jie-Zhi
authors.initials: JZ
authors.lastname: Zou
authors.forename: Ingemar
authors.initials: I
authors.lastname: Ernberg
authors.forename: Maria G
authors.initials: MG
authors.lastname: Masucci
authors.forename: Olle
authors.initials: O
authors.lastname: Ringden
authors.forename: Victor
authors.initials: V
authors.lastname: Levitsky
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000517:prevention & control; D005260:Female; D005500:Follow-Up Studies; D006680:HLA Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification / Q000502:physiology*; D006648:Histocompatibility / Q000276:immunology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000628:therapy; D047589:Leukocyte Reduction Procedures / Q000379:methods*; D008297:Male; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D013601:T-Lymphocytes / Q000473:pathology*; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous; D019562:Viral Load; D014766:Viremia / Q000821:virology*
chemical_list: D006680:HLA Antigens