The role of enzymes produced by white-rot fungus Irpex lacteus in the decolorization of the textile industry effluent.
The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus, without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or stationary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cultures shaken. Laccase activities were equivalent in both cultures and its level was not affected significantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase (RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly decreased by the stationary culture filtrate of 7 days in the absence of Mn (II) and veratryl alcohol. In the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymogram analysis.
Shin
Kwang-Soo
KS
Department of Microbiology, College of Sciences, Daejeon University Daejeon 300-716, Korea. shinks@dju.ac.kr
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D001592:Benzyl Alcohols / Q000378:metabolism; D001711:Biotransformation; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D007220:Industrial Waste; D042845:Laccase / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D010088:Oxidoreductases / Q000032:analysis; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D013783:Textile Industry; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001592:Benzyl Alcohols; D004396:Coloring Agents; D003470:Culture Media; D005656:Fungal Proteins; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D008345:Manganese; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D009195:Peroxidase; C042197:veratryl alcohol