title: [Isolation and culture of human pluripotent embryonic germ cells].
abstract: To establish human pluripotent embryonic germ (EG) cell lines, human primordial germ cells (PGCs) of embryos aborted in 5-9 week were cultured on inactive mouse STO fibroblast feeder. The medium contained human leukemia inhibitory factor (hLIF), human basic fibroblast growth factor (hbFGF) and forskolin. The EG cells could be passaged continuously until 12 generations. Most cells were positive in alkaline phosphatase staining and expressed cell surface antigen SSEA-3 and pluripotent marker Oct-4. These EG cell populations that retained normal karyotype could form embryoid body in culture and differentiate further into neuron-like cells, mucous epithelial cells, epithelial cells and other types of the cells spontaneously. These results indicated the cell clones derived from human PGCs resemble pluripotent EG cells from mouse PGCs in appearance or nature.
authors.affiliation: Laboratory of Tissue Engineering, Plastic Surgery, The 9th People's Hospital, Shanghai 2nd Medical University, Shanghai 200011.
authors.forename: Xiao Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Hsiao Chien
authors.initials: HC
authors.lastname: Cong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Chun Fang
authors.initials: CF
authors.lastname: Wu
authors.forename: Yi Lin
authors.initials: YL
authors.lastname: Cao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D050814:Octamer Transcription Factor-3; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; D000469:Alkaline Phosphatase