SNAP-25 in hippocampal CA1 region is involved in memory consolidation.
As a synaptosomal protein, SNAP-25 plays a role in a number of neuronal functions including axonal growth, dendrite formation, fusion of synaptic vesicles with membrane and the expression of long-term potentiation (LTP) in the hippocampus. Using a learning/memory behavior screening, we identified SNAP-25 as one of the differentially expressed genes in the hippocampus upon behavioral training. The inhibition of SNAP-25 with intracerebroventricular antisense oligonucleotide caused a deficit in long- but not short-term memory for step-down inhibitory avoidance. Intra-CA1 infusion of the SNAP-25 antisense oligonucleotide impaired long-term contextual fear memory and spatial memory and interfered with the LTP of synaptic transmission in the CA1 region. The inhibitory effect on LTP was not mediated by a pre-synaptic mechanism because paired pulse facilitation of synaptic transmission was not affected after administration of the antisense oligonucleotide. Together, the results suggest that SNAP-25 in the CA1 region is involved in memory consolidation.
Hou
Qiuling
Q
Laboratory of Higher Brain Functions, Institute of Neurobiology, Fudan University, Shanghai, China.
Gao
Xiang
X
Zhang
Xuehan
X
Kong
Lingwei
L
Wang
Xinming
X
Bian
Wei
W
Tu
Yanyang
Y
Jin
Meilei
M
Zhao
Guoping
G
Li
Baoming
B
Jing
Naihe
N
Yu
Lei
L
D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002478:Cells, Cultured; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation / Q000379:methods; D005239:Fear; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization / Q000379:methods; D007266:Inhibition, Psychological; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008565:Membrane Proteins / Q000502:physiology*; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013037:Spatial Behavior / Q000502:physiology; D050825:Synaptosomal-Associated Protein 25; D013997:Time Factors
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25