Scavenger receptor class B, type I mediates uptake of lipoprotein-associated phosphatidylcholine by primary porcine cerebrovascular endothelial cells.
We previously reported that scavenger receptor class B, type I (SR-BI) mediates uptake of lipoprotein-associated cholesteryl ester and Vitamin E by porcine brain capillary endothelial cells (pBCECs). In the present study we investigated whether SR-BI is capable of mediating phosphatidylcholine (PC) uptake by pBCECs from low- and high density lipoproteins. SR-BI-overexpressing CHO cells and pBCECs showed significantly enhanced uptake rates of PC from both lipoprotein classes. In addition, preincubation of pBCECs in the presence of both lipoprotein species resulted in a significant increase of the cellular fatty acid content, particularly linoleic and arachidonic acid. Our results suggest that uptake of lipoprotein-associated PC by the cerebrovasculature via SR-BI could generate a pool of lipids containing polyunsaturated fatty acids available for transport into deeper regions of the brain.
Sovic
Andrea
A
Institute of Molecular Biology and Biochemistry, Medical University Graz, Harrachgasse 21, A-8010 Graz, Austria.
Balazs
Zoltan
Z
Hrzenjak
Andelko
A
Reicher
Helga
H
Panzenboeck
Ute
U
Malle
Ernst
E
Sattler
Wolfgang
W
D000818:Animals; D018955:CD36 Antigens; D016466:CHO Cells; D002196:Capillaries / Q000166:cytology / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000502:physiology*; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D042783:Endothelial Cells / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000502:physiology*; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013552:Swine
D018955:CD36 Antigens; D016895:Culture Media, Serum-Free; D005231:Fatty Acids, Unsaturated; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B