A facile synthetic method to prepare fluorescently labeled ROMP polymers.
[structure: see text] To probe the activities of sperm ADAM protein (fertilinbeta), we devised a general synthetic strategy to generate fluorescently labeled fertilinbeta oligopeptide polymers. Immunofluorescence studies with these polymers demonstrated that fertilinbeta polymers bind specifically to a protein receptor on the mouse egg plasma membrane.
Roberts
Kenny S
KS
Department of Chemistry, State University of New York, Stony Brook, New York 11794-3400, USA.
Sampson
Nicole S
NS
D051722:ADAM Proteins; D000818:Animals; D002625:Chemistry, Organic / Q000379:methods*; D005260:Female; D000072196:Fertilins; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D008297:Male; D008562:Membrane Glycoproteins / Q000138:chemical synthesis / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000138:chemical synthesis* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis*; D009865:Oocytes / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D011108:Polymers; D011956:Receptors, Cell Surface; D051722:ADAM Proteins; D000072196:Fertilins; D008666:Metalloendopeptidases