Molecular cloning and characterization of SGT1.2, a novel splice variant of Homo sapiens SGT1.
SGT1.2, a novel splice variant of Homo sapiens SGT1 was isolated from a human fetal brain cDNA library. This cDNA is 1404 bp and contains an open reading frame from 68 to 1165 encoding a putative protein of 365 amino acids (SGT1.2) that share 90% identities to Homo sapiens SGT1, suppressor of G2 allele of SKP1 at protein level. RPS-BLAST searching revealed that SGT1.2 have a TPR domain, a p23 domain, a SGS domain and a CS domain. According to the search of the human genome database, SGT1.2 was mapped to human chromosome 13q14.13. Reversed transcription-polymerase chain reaction analysis indicated that it widely expressed in human adult tissues.
Zou
Xianqiong
X
a State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, P. R. China.
Ji
Chaoneng
C
Wang
Liu
L
Wu
Maoqing
M
Zheng
Huarui
H
Xu
Jian
J
Jin
Feng
F
Gu
Shaohua
S
Ying
Kang
K
Xie
Yi
Y
Mao
Yumin
Y
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D014644:Genetic Variation; D015186:Hominidae; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C490164:SUGT1 protein, human