Universal labeling chemistry for nucleic acid detection on DNA chips.
An efficient strategy for nucleic acid labeling and analysis on deoxyribonucleic acid (DNA) chips has been developed. This approach, which combines the fragmentation and the labeling steps, is based on the reactivity of the phosphates of DNA and ribonucleic acid (RNA) fragments and is using reporter molecules bearing a bromomethyl- or aryldiazomethane-reactive group. In this chapter, we describe the preparation of the reactive label and protocols for efficient labeling of any nucleic acid sequence, DNA or RNA, prior to their hybridization, detection, and analysis on DNA chips.
Laayoun
Ali
A
Advanced Technology/Molecular Diagnostics, BioMérieux, Marcy L'Etoile, France.
Bernal-Méndez
Eloy
E
Sothier
Isabelle
I
Kotera
Mitsuharu
M
Troesch
Alain
A
D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis
D009696:Nucleic Acids