title: Erythropoietin increases glutathione peroxidase enzyme activity and decreases lipid peroxidation levels in hypoxic-ischemic brain injury in neonatal rats.
abstract: BACKGROUND
  We have previously shown that erythropoietin (Epo) exerts neuroprotective effects in the Rice-Vannucci model of neonatal hypoxic-ischemic brain injury. However, the mechanisms of Epo protection in this model are still unclear.
  
  
  OBJECTIVE
  In the present study, we studied the effects of systemically administered Epo on lipid peroxidation levels and antioxidant enzyme (superoxide dismutase and glutathione peroxidase) activities following hypoxic-ischemic brain injury in neonatal rats.
  
  
  METHODS
  Seven-day-old Wistar rat pups were subjected to left carotid artery occlusion followed by 2.5 h of hypoxic exposure. Brain lipid peroxidation levels and antioxidant enzyme activities were measured in the injured hemispheres 24 h after the hypoxic-ischemic insult.
  
  
  RESULTS
  Hypoxic-ischemic injury significantly increased the thiobarbituric acid-reactive substance levels in the injured hemispheres as compared to the control group. In addition, glutathione peroxidase activity was significantly elevated in Epo-treated animals compared to saline-treated animals and the control group.
  
  
  CONCLUSIONS
  These results suggest that Epo exerts neuroprotective effects against hypoxic-ischemic brain injury at least partially via the modulation of antioxidant enzyme activity.
authors.affiliation: Department of Pediatrics, School of Medicine, Dokuz Eylul University, Inciralti, Izmir, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kumral
authors.forename: Sevil
authors.initials: S
authors.lastname: Gonenc
authors.forename: Osman
authors.initials: O
authors.lastname: Acikgoz
authors.forename: Atac
authors.initials: A
authors.lastname: Sonmez
authors.forename: Kursad
authors.initials: K
authors.lastname: Genc
authors.forename: Osman
authors.initials: O
authors.lastname: Yilmaz
authors.forename: Necati
authors.initials: N
authors.lastname: Gokmen
authors.forename: Nuray
authors.initials: N
authors.lastname: Duman
authors.forename: Hasan
authors.initials: H
authors.lastname: Ozkan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D004921:Erythropoietin / Q000008:administration & dosage*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D004921:Erythropoietin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase