title: Prefabrication of vascularized bone graft using guided bone regeneration.
abstract: This article describes the prefabrication of a vascularized bone graft composed of autologous particulate cancellous bone and marrow (PCBM), a vessel bundle, and a biodegradable membrane. The PCBM was placed around the saphenous vessel bundle of rats and rolled with a biodegradable membrane of L-lactide-epsilon-caprolactone copolymer to prepare the prefabricated vascularized bone graft (group A). As controls, combinations of PCBM and membrane (group B), vessel bundle and membrane (group C), and PCBM and vessel bundle (group D) were prepared. A radiographic study revealed radio-opacity in the implantation site of group A 1 week later, in contrast to the other groups. Newly formed bone in the membrane roll was histologically confirmed, and neomicrovasculature circulating from the vessel bundle through the newly formed bone tissue was observed. The increase in alkaline phosphatase activity and osteocalcin content was significant for the group A preparation compared with the other groups. We concluded that the combination of autologous PCBM, a vessel bundle, and a biodegradable membrane was promising in the prefabrication of vascularized bone with good blood circulation.
authors.affiliation: First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka 573-1121, Japan. e00543@cc.osaka-dent.ac.jp
authors.forename: Akishige
authors.initials: A
authors.lastname: Hokugo
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kubo
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Aoi
authors.initials: A
authors.lastname: Fukuda
authors.forename: Kaoru
authors.initials: K
authors.lastname: Horiuchi
authors.forename: Kozo
authors.initials: K
authors.lastname: Mushimoto
authors.forename: Shosuke
authors.initials: S
authors.lastname: Morita
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000295:instrumentation / Q000379:methods*; D001842:Bone and Bones / Q000098:blood supply* / Q000166:cytology / Q000601:surgery*; D002478:Cells, Cultured; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000502:physiology; D011091:Polyesters; D011474:Prosthesis Design; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods
chemical_list: D018786:Bone Substitutes; D008567:Membranes, Artificial; D011091:Polyesters; C062968:lactide-caprolactone copolymer