title: Surface topography modulates the osteogenesis in human bone marrow cell cultures grown on titanium samples prepared by a combination of mechanical and acid treatments.
abstract: Titanium samples of different roughness R(a) and morphology were prepared using a combination of mechanical (grinding with a SiC paper or blasting with aluminum oxide particles with 65 or 250 microm) and chemical (attack with a sulphuric acid based solution or a hydrofluoric acid based solution) treatments. The biological performance of the prepared surfaces was evaluated using human bone marrow osteoblastic cell cultures. Mechanically treated samples presented different R(a) values and surface morphology. The hydrofluoric acid solution was more effective than the sulphuric acid solution in smoothing titanium surface and also in eliminating aluminum contamination resulting from the blasting process. Bone marrow cells seeded on the different titanium samples showed a similar pattern of behavior during cell attachment and spreading. Cells proliferated very well on all the titanium surfaces and cell growth was observed during approximately two to three weeks. The samples treated with the hydrofluoric acid solution presented higher alkaline phosphatase activity. Only the blasted samples treated with the acid solutions allowed seeded bone marrow cells to form a mineralized extracellular matrix. The best biological performance was found in the blasted samples treated with the hydrofluoric acid solution, which could be related to the characteristic microtopography of these samples that presented a homogeneous and smooth roughness.
authors.affiliation: Metallurgical and Materials Science Department, Federal University of Rio de Janeiro, P.O. Box 68505, Rio de Janeiro, 21945-970, R.J., Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Diniz
authors.forename: G A
authors.initials: GA
authors.lastname: Soares
authors.forename: M J
authors.initials: MJ
authors.lastname: Coelho
authors.forename: M H
authors.initials: MH
authors.lastname: Fernandes