The solubilization and biological activities of Aspergillus beta-(1 --> 3)-D-glucan.
We have recently demonstrated that the cell wall beta-glucan of Candida albicans could be solubilized by sodium hypochlorite, followed by dimethylsulfoxide-extraction (NaClO-DMSO method). In this study, applying this method to Aspergillus spp., we prepared mycelial cell wall beta-glucan and examined its physical properties and immunotoxicological activity. The acetone-dried mycelia of Aspergillus spp. were oxidized by the NaClO-DMSO method. An analysis of (13)C NMR spectra revealed the preparations to be composed of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan. Also, the proportion of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan varied. Furthermore, a solubilized Aspergillus beta-glucan (ASBG) was prepared from OX-Asp by urea-autoclave treatment. ASBG showed limulus activity similar to Candida solubilized beta-glucan (CSBG), and there was little difference in the activity of ASBG between various Aspergillus spp. ASBG affected the production of IL-8 by human peripheral blood mononuclear cells (PBMC). ASBG should be useful for analyzing the clinical role of beta-glucan.
Ishibashi
Ken-ichi
K
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Miura
Noriko N
NN
Adachi
Yoshiyuki
Y
Tamura
Hiroshi
H
Tanaka
Shigenori
S
Ohno
Naohito
N
D000818:Animals; D001230:Aspergillus / Q000737:chemistry* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation; D009682:Magnetic Resonance Spectroscopy; D012973:Sodium Hypochlorite; D012995:Solubility; D047071:beta-Glucans / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology
D016209:Interleukin-8; D047071:beta-Glucans; C033363:beta-1,3-glucan; D012973:Sodium Hypochlorite; D004121:Dimethyl Sulfoxide