[Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-alpha].
OBJECTIVE
To investigate the role of p27 in the inhibition of emodin on the mesangial cell (MC) proliferation induced by tumor necrosis factor-alpha(TNF-alpha).


METHODS
p27 protein of MC was detected with western blotting analysis. The degree of MC proliferation was estimated through [(3)H] thymidine ([(3)H] TdR) incorporation. Different dosage of emodin (50 mg/L,100 mg/L) was added into MC stimulated by TNF-alpha.


RESULTS
TNF-alpha (200 kU/L) decreased p27 level of MC cultured in serum-free DMEM for 24 hours and increased[(3)H] TdR incorporation. Emodin increased p27 level of MC stimulated by TNF-alpha and decreased [(3)H] TdR incorporation. The more the emodin was added, the greater the above-mentioned effect of emodin.


CONCLUSIONS
The increment of p27 level maybe play an important role in the inhibition of emodin on MC proliferation induced by TNF-alpha.
Mei
Xiao-Bin
XB
Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China. xbmei@smmu.edu.cn
Yuan
Wei-Jie
WJ
Zhan
Feng-Lan
FL
Wu
Hao
H
Zhang
Xiao-Ying
XY
Cui
Ruo-Lan
RL
D000818:Animals; D018797:Cell Cycle Proteins / Q000032:analysis / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004642:Emodin / Q000037:antagonists & inhibitors*; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000502:physiology*
C493962:Cdkn1b protein, rat; D018797:Cell Cycle Proteins; D014409:Tumor Necrosis Factor-alpha; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004642:Emodin