title: Prooxidative effects of TEMPO on human erythrocytes.
abstract: The prooxidative effects of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) were observed in human erythrocytes. Incubation of red blood cells with the membrane-permeable TEMPO leads to a decrease in the concentration of intracellular reduced glutathione, accompanied by the reduction of TEMPO. Extracellular ferricyanide inhibited the loss of glutathione and reduction of TEMPO. TEMPO induced glutathione release from the cells and oxidation of hemoglobin to methemoglobin; ferricyanide prevented these effects. These results indicate that TEMPO may act as an oxidant to erythrocytes, whilst extracellular ferricyanide protects against its effects.
authors.affiliation: Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. abal@biol.uni.lodz.pl
authors.forename: Aneta
authors.initials: A
authors.lastname: Balcerczyk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Łuczak
authors.forename: Mirosław
authors.initials: M
authors.lastname: Soszyński
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; C003959:TEMPO