Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses.
Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 mer YEDVNGIRK (NP 165-173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various hantavirus sequences bound MAb E5/G6 consistently, despite the existence of several amino acid variations in this region. These results indicate that MAb E5/G6 is a useful tool for detecting hantavirus antigen in rodent or patient tissues using Western blotting or other immunohistochemical assays.
Okumura
M
M
Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Yoshimatsu
K
K
Araki
K
K
Lee
B-H
BH
Asano
A
A
Agui
T
T
Arikawa
J
J
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006476:Orthohantavirus / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000737:chemistry / Q000276:immunology*; D019590:Nucleocapsid Proteins
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D019590:Nucleocapsid Proteins; C100886:nucleocapsid protein, Puumala virus