Contribution of bone marrow-derived cells to skin: collagen deposition and wound repair.
The bone marrow provides inflammatory cells and endothelial progenitor cells to healing cutaneous wounds. To further explore the bone marrow contribution to skin and healing wounds, we used a chimeric mouse model in which the bone marrow from enhanced green fluorescent protein (EGFP) transgenic mice is transplanted into normal C57BL mice. We found that normal skin is a target organ for bone marrow-derived cells from both the hematopoietic and the mesenchymal stem cell pool. We present evidence that the bone marrow contribution to normal skin and the healing cutaneous wound is substantially greater than the previously recognized CD45+ subpopulation, where 15%-20% of the spindle-shaped dermal fibroblasts were bone marrow-derived (EGFP+). Furthermore, the bone marrow-derived cells were able to contract a collagen matrix and transcribe both collagen types I and III, whereas the skin-resident cells transcribed only collagen type I. Whereas endothelial progenitor cells were found early during the wound repair process, bone marrow-derived endothelial cells were not seen after epithelialization was complete. Our data show that wound healing involves local cutaneous cells for reconstituting the epidermis but distant bone marrow-derived cells and the adjacent uninjured dermal mesenchymal cells for reconstituting the dermal fibroblast population.
Fathke
Carrie
C
Department of Surgery, Box 356410, University of Washington Medical Center, 1959 NE Pacific Street, Seattle 98195, USA.
Wilson
Lynne
L
Hutter
Jonathan
J
Kapoor
Vishal
V
Smith
Andria
A
Hocking
Anne
A
Isik
Frank
F
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D019070:Cell Lineage / Q000276:immunology; D003094:Collagen / Q000096:biosynthesis*; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D020405:Dermis / Q000166:cytology / Q000276:immunology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000293:injuries; D018183:Transplantation Chimera; D014945:Wound Healing / Q000235:genetics / Q000276:immunology*
D024042:Collagen Type I; D024061:Collagen Type III; D049452:Green Fluorescent Proteins; D003094:Collagen; D017493:Leukocyte Common Antigens