title: Bax-inhibiting peptide derived from mouse and rat Ku70.
abstract: Bax is a proapoptotic protein that plays a key role in the induction of apoptosis. Ku70 has activities to repair DNA damage in the nucleus and to suppress apoptosis by inhibiting Bax in the cytosol. We previously designed peptides based on the amino acid sequence of Bax-binding domain of human Ku70, and showed that these peptides bind Bax and inhibit cell death in human cell lines. In the present report, we examined the biological activities of other pentapeptides, VPTLK and VPALR, derived from mouse and rat Ku70. Cells in culture accumulated FITC-labeled VPTLK and VPALR, indicating that these peptides are cell permeable (human, mouse, rat, and porcine cells were examined). These peptides bound to Bax and suppressed cell death in various cell types including primary cultured cells. These data suggest that such Bax inhibiting peptides from three mammalian species may be used to protect healthy cells from apoptotic injury under pathological conditions.
authors.affiliation: Blood Research Institute, The Blood Center of South Eastern Wisconsin and Department of Biochemistry, Medical College of Wisconsin, 8727 Watertown Plank Road, Milwaukee, WI 53226, USA.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tomioka
authors.forename: Takako
authors.initials: T
authors.lastname: Nagahara
authors.forename: Trudy
authors.initials: T
authors.lastname: Holyst
authors.forename: Motoshi
authors.initials: M
authors.lastname: Sawada
authors.forename: Paulette
authors.initials: P
authors.lastname: Hayes
authors.forename: Vivian
authors.initials: V
authors.lastname: Gama
authors.forename: Misako
authors.initials: M
authors.lastname: Okuno
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kanouchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasada
authors.forename: Demin
authors.initials: D
authors.lastname: Wang
authors.forename: Takanori
authors.initials: T
authors.lastname: Yokota
authors.forename: Eimei
authors.initials: E
authors.lastname: Sato
authors.forename: Shigemi
authors.initials: S
authors.lastname: Matsuyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005047:Etoposide / Q000494:pharmacology; D005260:Female; D006801:Humans; D000072200:Ku Autoantigen; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D017386:Sequence Homology, Amino Acid; D051028:bcl-2-Associated X Protein
chemical_list: D034961:Antigens, Nuclear; C494826:BAX protein, human; C494827:Bax protein, mouse; C494828:Bax protein, rat; D004268:DNA-Binding Proteins; D009842:Oligopeptides; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005047:Etoposide; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; C000606511:Xrcc6 protein, rat; D000072200:Ku Autoantigen