title: Identification of two novel HLA class II alleles, DQB1*0311 and DQB1*0620.
abstract: Two novel HLA class II alleles have been identified in routine typing of a kidney transplant patient and a cord blood unit from the Australian Cord Blood Bank in Sydney. Sequence analysis of exon 2 of the DQB1 genes revealed the novel polymorphism. A substitution of A to C at nucleotide position 136 has been identified for the DQB1*0311 allele when compared to the closest-matched allele, DQB1*030201. An identical substitution has also been identified for the DQB1*0620 allele when compared to the closest-matched allele, DQB1*0602. The substitution results in an amino acid change from methionine to leucine at position 46 implicating different specificity and affinity of antigen binding.
authors.affiliation: Molecular Genetics, Tissue Typing, Australian Red Cross Blood Service, Sydney, Australia.
authors.forename: J-P
authors.initials: JP
authors.lastname: Hogbin
authors.forename: W
authors.initials: W
authors.lastname: Greville
authors.forename: G
authors.initials: G
authors.lastname: Chapman
authors.forename: A
authors.initials: A
authors.lastname: Kennedy
authors.forename: Z M
authors.initials: ZM
authors.lastname: Velickovic
authors.forename: H
authors.initials: H
authors.lastname: Dunckley
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons / Q000235:genetics*; D014644:Genetic Variation; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D017931:DNA Primers; D006680:HLA Antigens