title: Apolipoprotein E4 allele and ribosomal genes in Alzheimer's disease.
abstract: Ribosomal genes are involved in cellular transcription, translation and gene expression modulation process. An association between 28S/18S rRNA ratio levels with apoptosis and aging has been reported. Moulder et al. [22] and Hashimoto et al. [8] showed an association between apolipoprotein E4 allele and neuronal cell apoptosis through diverse mechanisms. The apoE 4 allele is considered a late-onset Alzheimer's disease (AD) risk factor associated with AD pathogenesis. We evaluated the association between apoE4 allele genotyping by PCR and rRNA 28S/18S ratio by slot blotting technique using peripheral blood samples of 18 Alzheimer's disease patients, 18 elderly controls and 18 young controls. A rRNA ratio decrease was observed in AD individuals confirming our previous results but this association is independently of the ApoE4 allele genotype. Thus our results pointed that two different mechanisms are involved in the etiology of Alzheimer disease each one leading independently to cell death. Further studies could investigate these factors.
authors.affiliation: Disciplina de Biologia Molecular, Hemocentro FAMEMA, Faculdade de Medicina de Marília, São Paulo, Brasil.
authors.forename: Wagner Malagó
authors.initials: WM
authors.lastname: Tavares
authors.forename: Marcia Aparecida
authors.initials: MA
authors.lastname: Sperança
authors.forename: Roger Willian
authors.initials: RW
authors.lastname: de Labio
authors.forename: Clovis A
authors.initials: CA
authors.lastname: Peres
authors.forename: Ivan Hideyo
authors.initials: IH
authors.lastname: Okamoto
authors.forename: Paulo Henrique Ferreira
authors.initials: PH
authors.lastname: Bertolucci
authors.forename: Marília
authors.initials: M
authors.lastname: de A C Smith
authors.forename: Spencer Luiz Marques
authors.initials: SL
authors.lastname: Payão
mesh_terms: D000368:Aged; D000375:Aging / Q000502:physiology; D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D001057:Apolipoproteins E; D004275:DNA, Ribosomal