Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.
We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
Boyer
Jeanne
J
Unité de Génétique Moléculaire des Levures, URA2171 CNRS and UFR 927 Université Pierre et Marie Curie, Paris-Cedex 15, France. jboyer@pasteur.fr
Badis
Gwenaël
G
Fairhead
Cécile
C
Talla
Emmanuel
E
Hantraye
Florence
F
Fabre
Emmanuelle
E
Fischer
Gilles
G
Hennequin
Christophe
C
Koszul
Romain
R
Lafontaine
Ingrid
I
Ozier-Kalogeropoulos
Odile
O
Ricchetti
Miria
M
Richard
Guy-Franck
GF
Thierry
Agnès
A
Dujon
Bernard
B
D003001:Cloning, Molecular / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics / Q000502:physiology; D005804:Genes, Lethal / Q000235:genetics; D016153:Genes, Suppressor; D016681:Genome, Fungal; D016366:Open Reading Frames / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D016364:Reading Frames / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*; D014162:Transfection / Q000379:methods; D019076:Transgenes / Q000502:physiology
D004271:DNA, Fungal