The modelling of mononuclear phagocyte-connective tissue adhesion in vitro: application to disclose a specific inhibitory effect of Leishmania infection.
In this work, we have developed an adhesion assay to study interactions between mononuclear phagocytes and connective tissue in vitro and show its potential use to study diseases caused by intracellular microorganisms. The assay reproduces most of the characteristics of macrophage adhesion to connective tissue in vivo, such as: preferential adhesion to inflamed connective tissue, divalent cation and integrin dependence, and up-regulation upon cell activation. The phagocyte adhesion to connective tissue was inhibited by infection with Leishmania (58+/-22%, p < 0.05) and was not affected by infection with Mycobacterium or by endocytosis of latex beads. Manganese partially reverted the loss in adherence produced by Leishmania infection, indicating that the mechanisms regulating the function of integrins are affected by cell infection with Leishmania. This assay might be a useful tool for the study of the mechanisms by which mononuclear phagocytes play a role in the immune-inflammatory response and in the development of lesions.
Carvalhal
Djalma G F
DG
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão 121, 40295-001 Salvador, BA, Brazil.
Barbosa
Aryon
A
D'El-Rei Hermida
Micely
M
Clarencio
Jorge
J
Pinheiro
Nathanael F
NF
Veras
Patricia S T
PS
dos-Santos
Washington L C
WL
D000704:Analysis of Variance; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002413:Cations, Divalent / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003238:Connective Tissue / Q000378:metabolism* / Q000473:pathology; D046508:Culture Techniques; D007249:Inflammation / Q000378:metabolism; D016023:Integrins / Q000502:physiology; D007891:Leishmania / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D019907:Mycobacterium fortuitum / Q000502:physiology; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms
D015703:Antigens, CD; D002413:Cations, Divalent; D016023:Integrins; D008345:Manganese