[Immunocytochemical demonstration of astrocytes in brain sections combined with Nissl staining].
The aim of the present study was to develop an easy and reliable protocol of combined preparation staining, which would unite the advantages of immunocytochemical demonstration of astrocytes with the availability to evaluate functional state of neurons provided by Nissl technique. The presented protocol of paraffin sections processing allows to retain high quality of tissue structure and provides for selective demonstration of astrocytes using the monoclonal antibodies against glial fibrillary acidic protein and contrast Nissl staining of cells. The protocol can be used without any changes for processing of brain sections obtained from the humans and other mammals with the exception of mice and rabbits.
Korzhevskiĭ
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Otellin
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VA
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000473:pathology; D001921:Brain / Q000166:cytology* / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity; D013194:Staining and Labeling / Q000379:methods*