[Identification of acute leukemia-specific genes from leukemia recipient/sibling donor pairs by distinguishing study with oligonucleotide microarrays].
To explore differentially expressed genes in leukemia gene expression profile and identify main related genes in acute leukemia, gene expression profiles were analyzed in bone marrow/leucopheresis peripheral blood stem cells samples from 9 acute leukemia patients and their sibling donors with the use of oligonucleotide microarrays. 163 reported leukemia-related genes were involved in the study. The oligonucleotide primers were designed, synthesized and spotted on the chemical-material-coated-glass plates in array. The total RNAs were isolated from nine patients' bone marrow or leucopheresis peripheral blood cells and from nine their sibling donors peripheral blood stem cells treated by G-CSF, then collected by CS-3000 cell selection machine, and were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP. The mixed probes were then hybridized to the oligonucleotide microarray. The results showed that in four patient/donor pairs with B-ALL, 5 up-regulated (RIZ, STK-1, T-cell leukemia/lymphoma 1A, Cbp/p300, Op18) and 1 down-regulated genes (hematopoietic proteoglycan core protein) were identified; In five patient/donor pairs with AML-M(4) and AML-M(5), 6 up-regulated (STAT5B, ligand p62 for the Lck SH2, CST3, LTC4S, myeloid leukemia factor 2 and epb72) and 1 down-regulated genes (CCR5) were identified. In conclusion, on the basis of distinguishing study of specific genetic related recipient/sibling donor pairs, screening leukemia-related genes with oligonucleotide microarrays, a set of 13 up-regulated or down-regulated genes among 163 leukemia-related genes has been identified. The result has further confirmed that above genes play critical role in the molecular mechanism of acute leukemia.
Sun
Yi
Y
Hematopoietic Stem Cell Transplantation Center, Hospital 307, Academy of Military Medical Sciences, Beijing 100039, China.
Dong
Lu-Jia
LJ
Tian
Fang
F
Wang
Sheng-Qi
SQ
Jia
Zhi-Lin
ZL
Huang
Jian
J
Chen
Ze-Jian
ZJ
Li
Wu-Ju
WJ
Chen
Xi-Lin
XL
Zhu
Ping
P
D001782:Blood Donors; D004268:DNA-Binding Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008868:Microtubule Proteins / Q000235:genetics; D008894:Milk Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D036102:Peripheral Blood Stem Cell Transplantation; D010750:Phosphoproteins / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D050799:STAT5 Transcription Factor; D035781:Siblings; D050777:Stathmin; D015534:Trans-Activators / Q000235:genetics
D004268:DNA-Binding Proteins; D008868:Microtubule Proteins; D008894:Milk Proteins; D010750:Phosphoproteins; D050799:STAT5 Transcription Factor; C494095:STAT5B protein, human; C493995:STMN1 protein, human; D050777:Stathmin; D015534:Trans-Activators; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase