title: Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography.
abstract: A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
authors.forename: Ei-ichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
mesh_terms: D019659:Asteraceae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents; D018515:Plant Leaves / Q000737:chemistry; D012822:Silicon Dioxide
chemical_list: D007202:Indicators and Reagents; D012822:Silicon Dioxide