Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses.
The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works, 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenetic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenetic identification could be reached also with partial gyrB sequences of only 350 bp.
Demarta
A
A
Istituto Cantonale di Microbiologia, Via Mirasole 22A, 6500 Bellinzona, Switzerland. antonella.demarta@ti.ch
De Respinis
S
S
Dolina
M
M
Peduzzi
R
R
D015373:Bacterial Typing Techniques; D027081:DNA Gyrase; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D015007:Yersinia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase