title: Determination of dopamine by flow-injection analysis coupled with luminol-hexacyanoferrate(III) chemiluminescence detection.
abstract: A novel flow-injection method (FIA) for the determination of dopamine based on the inhibition of the intensity of chemiluminescence (CL) from luminol-hexacyanoferrate(III) system in basic medium is described. The present method allows the determination of dopamine over the range 30-100 microg l(-1) and 400-3000 microg l(-1). The relative standard deviation is 2.32% for 70 microg l(-1) dopamine and 1.22% for 1500 microg l(-1) dopamine (n = 20). The detection limit is 5 microg l(-1) with the sampling rate of 135 samples h(-1). This method has been applied for the determination of dopamine in commercial pharmaceutical injection samples. The results obtained by this method agreed with those by the official method.
authors.affiliation: Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Nalewajko
authors.forename: Rosa
authors.initials: R
authors.lastname: Bort Ramírez
authors.forename: Anatol
authors.initials: A
authors.lastname: Kojło
mesh_terms: D004298:Dopamine / Q000032:analysis* / Q000592:standards; D005292:Ferricyanides / Q000737:chemistry*; D017022:Flow Injection Analysis; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005292:Ferricyanides; D049408:Luminescent Agents; D004364:Pharmaceutical Preparations; C007931:hexacyanoferrate III; D008165:Luminol; D004298:Dopamine