N-nitrosations of basic amino acid residues in polypeptide.
Changes in the electrophoretic pattern were noted in the products of polypeptides of identical basic amino acids preincubated with reactive or degraded PN, suggesting the occurrence of N-nitrosation of the epsilon-amino group of lysine, the guanido group of arginine and the imidazole group of histidine. Additionally, increase in the N-nitroso immunoreactivity of preincubated histones H2A and H2B was detected by Western blot analysis.
Kuo
Wu-Nan
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA. kuow@cookman.edu
Ivy
Dynisha
D
Guruvadoo
Luvina
L
White
Atavia
A
Graham
Latia
L
D000818:Animals; D001120:Arginine / Q000737:chemistry; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000737:chemistry; D006657:Histones / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D014466:Ultraviolet Rays
D006657:Histones; D010455:Peptides; D011107:Polylysine; D006639:Histidine; D001120:Arginine; D008239:Lysine; D009584:Nitrogen