Photothermal spectrometry for detection in miniaturized systems: relevant features, strategies and recent applications.
There is a growing interest in using miniaturized analytical devices because they allow to execute the different steps of an analytical process within very short times and with drastic reduction in the amounts of solvents, reagents and samples. As for capillary electrophoresis, these systems require detectors which are sensitive, versatile and adaptable to very small detection volumes. In this respect, photothermal spectrometry which is complementary to fluorescence seems to be a promising detection method. This review describes the basic principle of photothermal spectrometry along with the related methods based on colinear-beam or crossed-beam configuration of the pump and probe lasers. Two experimental set ups especially designed for microfluidic systems as well as for capillary electrophoresis are described. Their characteristics and key features are discussed and the main applications are outlined.
Ghaleb
K Abbas
KA
Laboratoire des Sciences Analytiques, Université Claude Bernard-Lyon 1, 43 Boulevard du 11 Novembre 1918, Bâtiment CPE-308D, 69622 Villeurbanne Cedex, France.
Georges
J
J
D019075:Electrophoresis, Capillary; D004867:Equipment Design; D007834:Lasers; D046210:Microfluidic Analytical Techniques; D008853:Microscopy; D008904:Miniaturization; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D013696:Temperature