Sex difference and possible relationship to microvascular complications of serum prorenin levels in type 2 diabetic patients, measured by a novel antibody-activating direct enzyme kinetic assay.
Prorenin, a precursor of renin, was measured in sera from normal subjects and type 2 diabetic patients, using a newly developed antibody-activating direct enzyme kinetic assay. Levels of prorenin were examined in relationship to diabetic microangiopathies. The levels of antibody-activating direct prorenin (AAD-PR) were approximately 1.5-fold higher than levels of prorenin measured by a conventional enzyme-activating method. AAD-PR levels were significantly higher in males than in females and in diabetic patients than in normal subjects. Moreover, AAD-PR levels were higher in diabetic patients with microalbuminuria and even higher in those with macroalbuminuria. In normoalbuminuric diabetic patients, AAD-PR levels were higher in those with retinopathy. Furthermore, a significant positive correlation was seen between the AAD-PR levels and HbA(lc) in normoalbuminuric diabetic subjects without retinopathy. Thus, the determination of circulating serum prorenin measured as AAD-PR is related to glycemia and in type 2 diabetic patients may be a risk marker of diabetic microangiopathy. More studies are necessary to determine whether AAD-PR may actually predict the development of microangiopathy.
Kawazu
Shoji
S
Department of Total Health Management, Saitama Medical Center, Saitama Medical School, 1981 Kamodatsujido, Kawagoe, Saitama 350-8550, Japan. skawa@saitama-med.ac.jp
Minagawa
Shinya
S
Yazawa
Masako
M
Tsuchida
Atsuko
A
Ihara
Chika
C
Hosaka
Daiya
D
Ohmura
Eiji
E
Imai
Yasuo
Y
Suzuki
Fumiaki
F
Tanaka
Hiroshi
H
Ishida
Yuichi
Y
D000328:Adult; D000368:Aged; D015415:Biomarkers; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D003925:Diabetic Angiopathies / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D011237:Predictive Value of Tests; D012083:Renin / Q000097:blood* / Q000276:immunology; D012727:Sex Characteristics
D015415:Biomarkers; D012083:Renin