title: Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae.
abstract: The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.
authors.affiliation: GG&GD Municipal Health Service, Public Health Laboratory, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. sbruisten@gggd.amsterdam.nl
authors.forename: S M
authors.initials: SM
authors.lastname: Bruisten
authors.forename: G T
authors.initials: GT
authors.lastname: Noordhoek
authors.forename: A J C
authors.initials: AJ
authors.lastname: van den Brule
authors.forename: B
authors.initials: B
authors.lastname: Duim
authors.forename: C H E
authors.initials: CH
authors.lastname: Boel
authors.forename: K
authors.initials: K
authors.lastname: El-Faouzi
authors.forename: R
authors.initials: R
authors.lastname: du Maine
authors.forename: S
authors.initials: S
authors.lastname: Mulder
authors.forename: D
authors.initials: D
authors.lastname: Luijt
authors.forename: J
authors.initials: J
authors.lastname: Schirm
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D001425:Bacterial Outer Membrane Proteins; C082716:cppB protein, Neisseria gonorrhoeae