Effective inhibition of HIV-1 replication in cultured cells by external guide sequences and ribonuclease P.
We examined the suppressive effect of HIV-1 RNA gene cleavage on HIV-1 expression, using the catalytic RNA subunit RNase P and the 3'-half tRNA(Try) [external guide sequence (EGS)] in cultured cells. HIV-1 expression was inhibited by the tRNA(met)-EGS-U5 and U6-EGS-U5 from the tRNA(met) and U6 promoters, respectively. There was no difference in the inhibitory effects on HIV-1 expression between the tRNA(met) and U6 promoters.
Barnor
Jacob S
JS
Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Endo
Yumihiko
Y
Habu
Yuichiro
Y
Miyano-Kurosaki
Naoko
N
Kitano
Michiko
M
Yamamoto
Hirokazu
H
Takaku
Hiroshi
H
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012343:RNA, Transfer / Q000737:chemistry*; D012358:RNA, Transfer, Met / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D043262:Ribonuclease P / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*; D058727:RNA, Small Untranslated
D019380:Anti-HIV Agents; D012333:RNA, Messenger; D012358:RNA, Transfer, Met; D012367:RNA, Viral; D012343:RNA, Transfer; D043262:Ribonuclease P; D058727:RNA, Small Untranslated