[Study on loss of heterozygosity at microsatellite locus in larynx and pharynx squamous cell carcinoma and neck lymph nodes].
OBJECTIVE
To access prognosis and explore neck lymph nodes micrometastasis in larynx and pharynx squamous cell carcinoma (LPSCC) with loss of heterozygosity (LOH).


METHODS
By polymerase chain reaction (PCR), 18 cases of LPSCC and 72 resected neck lymph nodes were investigated with LOH at D9S171 locus.


RESULTS
The higher level of LOH (40.28%) was found in neck lymph nodes compared with the positive level of neck lymph nodes detected by pathological examination (8.33%) (P < 0.01); LOH was related with tumor differentiation in patients with LPSCC (P < 0.05). The LOH rate of primary tumor was closely correlated with the clinical stages (P < 0.01).


CONCLUSIONS
Our results suggested that analysis of LOH at D9S171 locus is one of sensitive means of evaluating neck lymph node micrometastasis, and which may be one of prognostic factors in patients with LPSCC.
Li
Binghua
B
Department of Otolaryngology, the Second Affiliated Hospital, College of Medical Sciences, Zhejiang University, Hangzhou, 310009, China.
Xu
Yaping
Y
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D009333:Neck; D010610:Pharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis