title: Tryptic peptides of canine thyroglobulin reactive with sera of patients with canine hypothyroidism caused by autoimmune thyroiditis.
abstract: Canine thyroglobulin (cTg) was treated with trypsin at a ratio of trypsin to cTg of 1:100 (w/w). Tryptic peptides of cTg were analysed by Western immunoblotting for their reactivity to serum thyroglobulin autoantibodies (TgAA) from patients with TgAA-positive hypothyroidism and normal individuals. The sera of patients with TgAA-positive hypothyroidism reacted with several peptides: 43, 32.5 and 31 kDa; the sera of normal individuals did not bind these tryptic peptides. Some of the TgAA-positive sera of patients reacted with 25 kDa peptide in addition to three tryptic peptides above. This experiment was the first report about antigenic epitopes of cTg. These small tryptic peptides recognized by TgAA may be related with the induction of TgAA and may be useful as markers for autoimmune thyroid diseases in dog.
authors.affiliation: Department of Clinical Veterinary Science, The United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. s01060@st.obihiro.ac.jp
authors.forename: J-Y
authors.initials: JY
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Uzuka
authors.forename: S
authors.initials: S
authors.lastname: Tanabe
authors.forename: T
authors.initials: T
authors.lastname: Takasawa
authors.forename: T
authors.initials: T
authors.lastname: Sarashina
authors.forename: R F
authors.initials: RF
authors.lastname: Nachreiner
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D007037:Hypothyroidism / Q000097:blood / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D013954:Thyroglobulin / Q000276:immunology* / Q000378:metabolism; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D014357:Trypsin
chemical_list: D001323:Autoantibodies; D013954:Thyroglobulin; D014357:Trypsin