Electroacupuncture ameliorates experimental colitis induced by acetic acid in rat.
The effect of electroacupuncture (EA) on experimental colitis was investigated in Sprague-Dawley rats. Colitis was induced by intracolonic instillation of 4% acetic acid. EA (2 Hz, 0.05 ms, 2 V for 20 min) was applied to bilateral Hoku (LI-4) and Zusanli (ST-36) on 12 hrs and 36 hrs after induction of colitis. EA-treatment significantly reduced the macroscopic damage and the myeloperoxidase activity of colonic samples at 3 days post-induction of colitis. Colitic colon showed a decreased in vitro motility. However, colonic motility of EA-treated group was not significantly different from that of normal group. The anti-inflammatory effect of EA was not inhibited by a glucocorticoid receptor antagonist, RU-486, but suppressed by a beta-adrenoceptor antagonist, propranonol. These results suggest that EA-treatment has a beneficial effect on colitis, and its anti-inflammatory effect is mediated by beta-adrenoceptor activation but not by endogenous glucocorticoiddependent mechanism.
Kang
Jeoung Woo
JW
Department of Physiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Kim
Tae Wan
TW
La
Jun Ho
JH
Sung
Tae Sik
TS
Kim
Hyun Ju
HJ
Kwon
Young Bae
YB
Kim
Jeum Yong
JY
Yang
Il Suk
IS
D019342:Acetic Acid; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D015671:Electroacupuncture / Q000662:veterinary*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000502:physiology; D006727:Hormone Antagonists / Q000494:pharmacology; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000319:Adrenergic beta-Antagonists; D018679:Cholinergic Agonists; D004791:Enzyme Inhibitors; D006727:Hormone Antagonists; D015735:Mifepristone; D002217:Carbachol; D011433:Propranolol; D009195:Peroxidase; D019342:Acetic Acid; D019331:NG-Nitroarginine Methyl Ester