title: Positive contribution of hydration on DNA binding by E2c protein from papillomavirus.
abstract: Protein-nucleic acid interactions are responsible for the regulation of key biological events such as genomic transcription and recombination and viral replication. However, the recognition mechanisms involved in these processes are not completely understood. Here, we investigate the dominant forces involved in protein-protein and protein-DNA interactions for the 80-amino-acid C-terminal domain of the E2 protein (E2c) from human papillomavirus (HPV-16). The E2c protein is a homodimer that specifically binds to double-stranded DNA containing the consensus sequence ACCG-N(4)-CGGT, where N is any nucleotide. DNA binding affinity is reduced by lowering water chemical potential, accompanied by an increase in cooperativity. Wyman linkage relations between affinity and water chemical potential indicate that 11 additional water molecules are bound in the formation of the complex between E2c and DNA. Salt dissociation isotherms showed that 10 counterions are released upon association, even at low water activity, indicating that this latter variable does not change the electrostatic component of the interaction. Further analysis demonstrates a strong dependence of cooperativity of binding on the protein concentration. Altogether, these results reveal a novel binding pathway in which the consolidated complex may achieve its final form via a monomer-DNA intermediate, which favors the binding of a second monomer. This molecular mechanism reveals the contributions of multiple conformers in a tight virus genome modulation that seems to be important in the cell infection scenario.
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, CCS, Bss34, Ilha do Fundão, 21941-590 Rio de Janeiro, Brazil. mauricio@pharma.ufrj.br
authors.forename: Luis Maurício T R
authors.initials: LM
authors.lastname: Lima
authors.forename: Jerson L
authors.initials: JL
authors.lastname: Silva
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D027383:Papillomaviridae / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D013816:Thermodynamics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009841:Oligonucleotides; D021122:Protein Subunits; D014764:Viral Proteins; D014867:Water; D004247:DNA; D005990:Glycerol