A recombinant plant natriuretic peptide causes rapid and spatially differentiated K+, Na+ and H+ flux changes in Arabidopsis thaliana roots.
Plant natriuretic peptides (PNPs) belong to a novel class of systemically mobile molecules that are structurally similar to the N-terminal domain of expansins and affect physiological processes such as protoplast volume regulation at nano-molar concentrations. Here we demonstrate that AtPNP-A, a recombinant Arabidopsis thaliana PNP causes rapid H(+) influx in the elongation zone of A. thaliana roots but not in the mature zone. AtPNP-A also induces significant K(+) and Na(+) efflux and this effect is seen in the mature root zone only. These observations suggest that responses to AtPNP-A are developmental stage and tissue specific and point to a complex role in plant growth and homeostasis.
Ludidi
Ndiko
N
University of the Western Cape, Department of Biotechnology, Private Bag X17, Bellville, 7535, South Africa.
Morse
Monique
M
Sayed
Muhammed
M
Wherrett
Timothy
T
Shabala
Sergey
S
Gehring
Chris
C
D000595:Amino Acid Sequence / Q000502:physiology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002414:Cations, Monovalent / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D006706:Homeostasis / Q000187:drug effects / Q000235:genetics; D017136:Ion Transport / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011522:Protons; D011930:Reaction Time / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013997:Time Factors
D029681:Arabidopsis Proteins; D002414:Cations, Monovalent; D045265:Natriuretic Peptides; C464762:PNP-A protein, Arabidopsis; D011522:Protons; D011994:Recombinant Proteins; D012964:Sodium; D011188:Potassium