Inhibitory effects of extracts from peels of Citrus natsudaidai encapsulated in hybrid liposomes on the growth of tumor cells in vitro.
Inhibitory effects of extracts from peels of Citrus natsudaidai (natsumikan) encapsulated in hybrid liposomes (HL) composed of L-alpha-dimyristoylphosphatidylcholine and polyoxyethylene (20) sorbitan monolaurate on the growth of tumor cells were examined. The extracts with lower polar solvents inhibited the growth of B-16 mouse melanoma and human lung carcinoma cells, although the extracts with higher polar solvents showed no antitumor activity. In particular, the inhibitory effects of extracts with lower polar solvents encapsulated in HL were enhanced as compared with those of free extracts. Fluorescence microscopic analysis indicated that the HL including petroleum ether extracts induced apoptosis in B-16 mouse melanoma cells. On the other hand, the viability of normal human fibroblast cells was even less affected by the extracts of natsumikan. These results suggest that hydrophobic antitumor agents should be present in peels of natsumikan.
Kadota
Yutaka
Y
Division of Applied Chemistry, Graduate School of Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Taniguchi
Chiho
C
Masuhara
Shigeo
S
Yamamoto
Shinjiro
S
Furusaki
Shintaro
S
Iwahara
Masayoshi
M
Goto
Koichi
K
Matsumoto
Yoko
Y
Ueoka
Ryuichi
R
D000230:Adenocarcinoma; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D008081:Liposomes; D008545:Melanoma; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013274:Stomach Neoplasms
D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D010936:Plant Extracts