Bacterial thymidylate synthase with intein, group II Intron, and distinctive ThyX motifs.
The ThyX class of thymidylate synthases was previously characterized by a common ThyX motif, RHRX7S. We report bacterial ThyX sequences having distinctive ThyX motifs, suggesting a more general ThyX motif, R/THRX7-8S. One ThyX sequence has an intein in its ThyX motif that was shown to do protein splicing and a group II intron in its gene, suggesting a hot spot for these self-splicing mobile elements.
Liu
Xiang-Qin
XQ
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada. pxqliu@dal.ca
Yang
Jing
J
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D016415:Sequence Alignment; D013302:Streptomyces / Q000235:genetics; D020124:Thermotoga maritima / Q000235:genetics; D013940:Thymidylate Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D013940:Thymidylate Synthase