title: [Studies on calcium sensitive period during floral differentiation of cucumber cotyledonary node cultures in vitro].
abstract: Cotyledonary nodes of cucumber cultured on calcium-free medium for 0, 1, 2, 3, 4, 5, 6d respectively, were transferred to medium with 6.0 mmol/L CaCl2 for 24h, then returned to calcium-free medium. Cotyledonary nodes cultured on calcium-free or 6.0 mmol/L CaCl2 medium for all time, were taken as controls. Results showed that cotyledonary nodes were transferred to 6.0 mmol/L CaCl2 medium for 24h during 0-3d after the beginning of culture, percentage of floral bud formation at cotyledonary nodes was increased significantly. Transferring cotyledonary nodes on the 3d day after the beginning of culture was achieved best effect, percentage of floral bud formation was up to 34.3%. We deduced that the calcium sensitive period during floral differentiation of cucumber cotyleddonary node cultured in vitro may be 0-4d after the beginning of culture.
authors.affiliation: School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
authors.forename: Li Lin
authors.initials: LL
authors.lastname: Wang
authors.forename: Ji Liang
authors.initials: JL
authors.lastname: Pang
authors.forename: Jiang Qin
authors.initials: JQ
authors.lastname: Hu
authors.forename: Yan Yoan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Hai Man
authors.initials: HM
authors.lastname: Liang
mesh_terms: D002122:Calcium Chloride / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D018548:Cotyledon / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D018553:Cucumis sativus / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D035264:Flowers / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development
chemical_list: D002122:Calcium Chloride