title: Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase.
abstract: Inhibitors of melanin biosynthesis were screened by using three different methods. The extract of Veratrum patulum contains hydroxystilbene compounds that are potent tyrosinase inhibitors. We evaluated the enzyme inhibitory property on the mushroom tyrosinase of hydroxystilbene compounds including resveratrol, oxyresveratrol, and their analogs. Biotransformation using cellulase of the whole extract brought about an increase in the inhibitory activity of the products on mushroom tyrosinase. The enhancement of tyrosinase inhibition is supposed to increase the concentration of aglycon, which has superior inhibitory activity to its glycoside. Eventually, melanin biosynthesis was inhibited by the enhanced tyrosinase inhibitory activity of the extract. This result indicated that deglycosylation of stilbene compounds has exerted more effective inhibition on the enzyme than that of the unprocessed plant extract.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Baek
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Seo
authors.forename: Yung Hee
authors.initials: YH
authors.lastname: Kho
authors.forename: Tae Kwang
authors.initials: TK
authors.lastname: Oh
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000201:enzymology; D001711:Biotransformation; D002480:Cellulase / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008543:Melanins / Q000096:biosynthesis*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D013267:Stilbenes / Q000378:metabolism / Q000494:pharmacology*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*; D014703:Veratrum / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D008543:Melanins; D010936:Plant Extracts; D013267:Stilbenes; D014442:Monophenol Monooxygenase; D002480:Cellulase