[Construction and immune potency of recombinant adenovirus containing codon-modified HIV-1 gp120].
BACKGROUND
To construct replication-deficient recombinant adenovirus expressing wild and codon-modified HIV-1 gp120.


METHODS
The viral codons were changed to the codon usage of highly expressed mammal gene, the resulting modified gp120 gene was synthesized. The wild and modified gp120 genes were cloned into shuttle vector pShuttle-CMV respectively, and then the constructed plasmids containing gp120 gene was cotransformed with the backbone vector pADeasy-1 into E.coli BJ5183. Transfection of the recombinant AdEasy plasmid into 293 cells was performed to obtain recombinant adenoviruses. The mice were immunized with the recombinant adenoviruses. Their immunogenicity was evaluated by testing antibody and CTL levels of immunized mice.


RESULTS
Two strains of recombinant adenovirus expressing wild and codon-modified HIV-1 gp120 were obtained. The protein expressing level of the recombinant adenoviruses containing modified genes was much higher than that containing wild genes. The mice immunized with recombinant adenoviruses elicited HIV-1 specific antibody and CTL response. The rAd-mod gp120 group was better than the rAd-wt gp120 group.


CONCLUSIONS
Replication-deficient recombinant adenovirus expressing HIV-1 gp120 can elicit HIV-1 specific humoral and cellular response, the codon-modified recombinant virus was more efficient than the native.
Feng
Xia
X
Department of Oncogenic Virus and HIV, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Yu
Shuang-qing
SQ
Chen
Guo-min
GM
Zuo
Jian-min
JM
Zhou
Ling
L
Zeng
Yi
Y
D016915:AIDS Vaccines / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D003062:Codon / Q000235:genetics; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D016915:AIDS Vaccines; D003062:Codon; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120