Protein kinase B, P34cdc2 kinase, and p21 ras GTP-binding in kidneys of aging rats.
Renal nephropathy present in male Wistar rats more than 13 months of age was reported as an indication that the rats were in renal failure. In this study, the renal tissue damage at 14 months of age in male Munich Wistar rats was similar to that reported for Wistar rats, indicating that Munich Wistar rats could be another model for study of kidney function in the aging rat. The usual renal response to injury involves increased cell division and/or reparative processes that involve tyrosine kinase activity (TyrK) and/or guanosine triphosphate-binding (G) protein signal trans-duction pathways. This study reveals the presence of renal tissue damage coinciding with significantly reduced activity of Ras, Akt, and p34cdc2 kinase, the signaling proteins that regulate cell division and/or growth, in renal cortical tissues of aging rats compared to young rats (P < 0.005, P < 0.005, and P< 0.001, respectively). These results suggest that proteins involved in signal transduction pathways associated with cell replication are downregulated in the aging kidney cortex at a time when renal cellular damage is also present.
Parekh
Vipul V
VV
Department of Physiology and Biophysics, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA.
Falcone
Jeff C
JC
Wills-Frank
Lisa A
LA
Joshua
Irving G
IG
Dholakia
Jaydev N
JN
Passmore
John C
JC
D000375:Aging; D000818:Animals; D001835:Body Weight; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002455:Cell Division; D019926:Cyclin B / Q000378:metabolism; D015151:Immunoblotting; D007668:Kidney / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D007672:Kidney Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009929:Organ Size; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D019926:Cyclin B; D011518:Proto-Oncogene Proteins; C494920:Akt1 protein, rat; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D016203:CDC2 Protein Kinase; D016283:Proto-Oncogene Proteins p21(ras)