Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays.
Label-free detection emerges as a new approach in the development of technologies for cell-based screening assays. Unlike the classic detection methods that use fluorescence, radioisotope, luminescence, or light absorption, label-free detection directly measures the cell function without using a labeled molecule. The advantages of label-free detection include a simple homogeneous assay format, noninvasive measurement, less interference with normal cell function, kinetic measurement, and reduced time for assay development. Here, we have applied the electrical impedance detection method in a real-time cell electronic sensing (RT-CES trade mark ) system for cell-based assays. The cell growth rate measured by this RT-CES system was comparable to actual cell number counted manually. In addition, cell proliferation, cytotoxicity, cytoprotection, cell growth inhibition, and apoptosis data generated by this RT-CES system correlated with those determined by the classic methods. The conclusion is that the RT-CES system is a useful tool for label-free detection of certain cell-based parameters.
Solly
Kelli
K
Department of Automated Biotechnology, Merck & Co., North Wales, PA, USA.
Wang
Xiaobo
X
Xu
Xiao
X
Strulovici
Berta
B
Zheng
Wei
W
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003199:Computer Systems; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008839:Microelectrodes; D012680:Sensitivity and Specificity
D004317:Doxorubicin