title: [Re-growth of induced-osseous tissue encapsulated in muscular fasciae for prefabrication of an osteo-musculo-cutaneous flap].
abstract: OBJECTIVE
  To improve the survival of mesenchymal stem cells (MSCs) for prefabrication of an osteo-musculo-cutaneous flap.
  
  
  METHODS
  In a mouse model, the compound of MSCs and p(3HB-co-3HH) were embeded in the latissimus dorsi muscle as an experimental group and the muscle pocket of the buttock as the control. The examinations of the HE staining, hybridization in situ of osteonectin mRNA and Von kossa staining were used to evaluate the results.
  
  
  RESULTS
  The expression of osteonectin mRNA and the Von kossa staining showed that the latissimus dorsi muscle group was superior to the control in 2 weeks and 4 weeks after the surgery in vivo.
  
  
  CONCLUSIONS
  The results indicate that the above-mentioned technique may be a good alternative for the prefabrication of the osteo-musculo-cutaneous flap.
authors.affiliation: Surgery Hospital Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Ying-ling
authors.initials: YL
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Qun
authors.initials: Q
authors.lastname: Qiao
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Qi
mesh_terms: D000818:Animals; D005205:Fascia / Q000637:transplantation*; D006651:Histocytochemistry / Q000379:methods; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D033581:Stem Cell Transplantation / Q000379:methods; D013524:Surgical Flaps / Q000098:blood supply*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015676:Osteonectin; D012333:RNA, Messenger