[The identification of mouse cloned SFA DNA].
For some basic investigation and the construction of artificial chromosomes, cloned centromeric DNAs identified on a firm ground are required. Thus, in the present work a preliminary screened clone of 13.5 kb DNA, 6# clone, form a mouse centromeric library contructed previously in our library was futher investigated by FISH and PCR. It was found that mouse 6# cloned SFA DNA, as shown by FISH is a fragment of mouse centromeric DNA. Evidence was also observed that 6# cloned SFA DNA consists of mouse minor satellite DNA and other DNA sequences.
Yi
Ning
N
Institute of Biochenistry and Cell Biology, Academia Sinica, Shanghai 200031.
Wu
Weng Qing
WQ
Ni
Zu Mei
ZM
Shi
Lu Ji
LJ
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite; D004247:DNA