[ELISA kit for detection of biliary vesicular protein and its preliminary clinical application].
OBJECTIVE
To establish a rapid and precise detective method of 33.5 kd vesicular protein and to screen an effective treatment of cholelithiasis.


METHODS
Specific antibody of the biliary vesicular protein was obtained by immunizing rabbits and enzyme-linked immunosorbent assay (ELISA) kit was developed. The concentrations of 33.5 kd vesicular protein in serum and bile of gallstone patients and control were examined respectively. The effects of Cholagogue Dry Syrup and Eulektrol Capsule on decreasing 33.5 kd vesicular protein were also studied by ELISA kit.


RESULTS
One-step ELISA equation was Y=0.035 X (r=0.99). The vesicular protein concentrations in serum and bile of cholesterol gallstone group [(179.8+/-97.9) mg/L and (213.4+/-70.1) mg/L respectively] were significantly (P<0.05) higher than in the pigment stone group and control. Data showed that, with 2-week administration, Cholagogue Dry Syrup significantly decreased both biliary and serum 33.5 kd vesicular protein of cholesterol gallstone patients, while Eulekrol Capsule and control groups didn't have the same results.


CONCLUSIONS
The concentrations of 33.5 kd protein are different in cholesterol gallstone patients and healthy groups which might be related to cholesterol nucleation process. Cholagogue Dry Syrup is of cholagogic and litholytic effect by decreasing biliary lithogenesis.
Cai
Duan
D
Surgery Department, Huashan Hospital, Fudan University, Shanghai 200040, China. caiwei2000@online.sh.cn
Xiang
Jian-Bin
JB
Zhang
Yan-Ling
YL
Ma
Bao-Jin
BJ
D001659:Biliary Tract / Q000187:drug effects / Q000378:metabolism*; D002769:Cholelithiasis / Q000188:drug therapy / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D011506:Proteins