[Transplantation of adrenocortical cells in allorat using porous PHB as cell carrier].
This experiment was designed to investigate the feasibility of transplanatation of using porous PHB as cell carrier for the transplanatation of adrenocortical cells. Adrenocortical cells from rat adrenal gland were separated and cultured in vitro. The effect of PHB on the proliferation and secretory function of adrenocortical cells were evaluated by MTT and RIA methods. Then adrenocortical cells were seeded into porous PHB. After the cells were cultured in vitro for about seven days, they were implanted into the rats having undergone bilateral adrenalectomy. The changes of blood corticosterone and aldosterone and the local histological changes in these rats were observed. Adrenocortical cells were able to grow and survive on PHB. No effect on the proliferation and secretory function of adrenocortical cells were observed. Most bilateral adrenalectomized rats bearing the transplanted adrenocortical cells within PHB (study group) survived longer than did the adrenalectomized rats in control group. The blood corticosterone level and aldosterone level of study group were higher than those of control group. It was found that PHB has no effects on the survival, proliferation and secretory function of adrenocortical cells. Adrenocortical cells within PHB can survive a period of time and can secrete corticosterone and aldosterone which can meet the needs of the adrenalectomized rats. PHB can degrade slowly in vivo. It is feasible to perform transplantation of adrenocortical cells using porous PHB as cell carrier.
Li
Wei
W
Department of Urology, The Renmin Hospital of Wuhan University, Wuhan 430060, China.
Hu
Ping
P
Zhang
Xiaobin
X
Gao
Feng
F
Zhang
Jie
J
Chen
Fan
F
Jin
Huamin
H
D000302:Adrenal Cortex / Q000166:cytology*; D000450:Aldosterone / Q000097:blood / Q000652:urine; D000818:Animals; D001673:Biodegradation, Environmental; D049109:Cell Proliferation; D017690:Cell Transplantation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003345:Corticosterone / Q000097:blood* / Q000652:urine; D006885:Hydroxybutyrates / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D000094122:Polyhydroxybutyrates
D000450:Aldosterone; D003345:Corticosterone; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates