Peptide separation by Hydrophilic-Interaction Chromatography: a review.
Recent developments in the separation of peptides by high-performance liquid chromatography (HPLC) using polar sorbents with less polar eluents are summarized in this review. This separation mode is now commonly referred to as Hydrophilic-Interaction Chromatography (HILIC). The retention mechanism and chromatographic behavior of polar solutes under HILIC conditions are studied on TSKgel Amide-80 columns, which consist of carbamoyl groups bonded to a silica gel matrix, using a mixture of acetonitrile (MeCN)-water containing 0.1% trifluoroacetic acid (TFA). Some applications are given in peptide field using Hydrophilic-Interaction Chromatography.
Yoshida
Tatsunari
T
Tosoh Analysis and Research Center, 2743-1 Hayakawa, Ayase-shi, Kanagawa 252-1123, Japan.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*
D000596:Amino Acids; D010455:Peptides