A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium sp. & Flavobacterium sp. and their potentialities as biofertilizer.
A number of nitrogen fixing bacteria has been isolated from forest phyllosphere on the basis of nitrogenase activity. Among them two best isolates are selected and identified as Corynebacterium sp. AN1 & Flavobacterium sp. TK2 able to reduce 88 and 132 n mol of acetylene (10(8)cells(-1)h(-1)) respectively. They were grown in large amount and sprayed on the phyllosphere of maize plants as a substitute for nitrogenous fertilizer. Marked improvements in growth and total nitrogen content of the plant have been observed by the application of these nitrogen-fixing bacteria. An average 30-37% increase in yield was obtained, which is nearer to chemical fertilizer treatment. Comparatively better effect was obtained by application of Flavobacterium sp.
Giri
S
S
Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University Midnapore-721102, West Bengal, India.
Pati
B R
BR
D003352:Corynebacterium / Q000201:enzymology / Q000302:isolation & purification*; D005308:Fertilizers; D005417:Flavobacterium / Q000201:enzymology / Q000302:isolation & purification*; D009586:Nitrogen Fixation; D018515:Plant Leaves / Q000382:microbiology*; D014197:Trees; D003313:Zea mays / Q000254:growth & development
D005308:Fertilizers