title: A mannose binding protein is involved in the adherence of Acanthamoeba species to inert surfaces.
abstract: Some carbohydrates are known to decrease the attachment of Acanthamoeba sp. to biological surfaces. By a method based on the reduction of a tetrazolium salt (XTT) by the mitochondrial dehydrogenases of the parasites, d-mannose and alpha-d-mannopyranoside have been shown to reduce Acanthamoeba attachment to inert surfaces, indicating that the mannose binding protein of Acanthamoeba trophozoites is involved in adherence to inert surfaces. The reduction in attachment is dose dependant and is not linked with a potential toxicity of the carbohydrates. All the species of Acanthamoeba tested were concerned by this mannose binding protein, but the adhesion of A. culbertsoni was also reduced by the presence of glucose.
authors.affiliation: Unité de recherche en biologie parasitaire et fongique, Laboratoire de parasitologie et mycologie médicales, CHU La Milétrie, 86021 Poitiers Cedex, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Imbert-Bouyer
authors.forename: Annabelle
authors.initials: A
authors.lastname: Merlaud
authors.forename: Christine
authors.initials: C
authors.lastname: Imbert
authors.forename: Gyslaine
authors.initials: G
authors.lastname: Daniault
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Rodier
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism*; D000268:Adhesiveness; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*
chemical_list: D037601:Mannose-Binding Lectin; D015800:Protozoan Proteins; D005947:Glucose; D008358:Mannose