DNA microarray analysis of dysplastic morphology associated with acute myeloid leukemia.
OBJECTIVE
Acute myeloid leukemia (AML) develops de novo or secondarily to either myelodysplastic syndrome (MDS) or anticancer treatment (therapy-related leukemia, TRL). Prominent dysplasia of blood cells is apparent in individuals with MDS-related AML as well as in some patients with TRL or even with de novo AML. The clinical entity of AML with multilineage dysplasia (AML-MLD) is likely to be an amalgamation of MDS-related AML and de novo AML-MLD. The aim of this study was to clarify, by the use of high-density oligonucleotide microarrays, whether these subcategories of AML are intrinsically distinct from each other.


METHODS
The AC133+ hematopoietic stem cell-like fractions were purified from the bone marrow of individuals with de novo AML without dysplasia (n = 15), AML-MLD (n = 11), MDS-related AML (n = 11), or TRL (n = 2), and were subjected to the synthesis of cRNA which was subsequently hybridized to microarray harboring oligonucleotide corresponding to more than 12,000 probe sets.


RESULTS
We could identify many genes whose expression was specific to these various subcategories of AML. Furthermore, with the correspondence analysis/three-dimensional projection strategy, we were able to visualize the independent, yet partially overlapping, nature of current AML subcategories on the basis of their transcriptomes.


CONCLUSIONS
Our data indicate the possibility of subclassification of AML based on gene expression profiles of leukemic blasts.
Tsutsumi
Chizuko
C
Department of Hematology and Molecular Medicine Unit, Nagasaki University, Nagasaki, Japan.
Ueda
Masuzu
M
Miyazaki
Yasushi
Y
Yamashita
Yoshihiro
Y
Choi
Young Lim
YL
Ota
Jun
J
Kaneda
Ruri
R
Koinuma
Koji
K
Fujiwara
Shin-ichiro
S
Kisanuki
Hiroyuki
H
Ishikawa
Madoka
M
Ozawa
Keiya
K
Tomonaga
Masao
M
Mano
Hiroyuki
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification* / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis