title: Importance of platelets and fibrinogen in neutrophil-endothelial cell interactions in septic shock.
abstract: OBJECTIVE
  To examine the role of platelets, fibrin, and adhesion molecules in mediating neutrophil-endothelial cell interactions in septic shock.
  
  
  METHODS
  Controlled experiments using phase contrast microscopy to examine neutrophil, platelet, and endothelial cell interactions in flowing cell suspensions under simulated physiologic conditions.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Adult patients with septic shock and normal volunteers.
  
  
  METHODS
  Microslides were coated with human umbilical vein endothelial cells. Neutrophils were removed from control subjects and patients in septic shock and were perfused over endothelial cells at rates representing a range of physiologic shear stresses. In an attempt to examine the effects of fibrin deposition on neutrophil-endothelial cell interactions, neutrophils, with and without platelets, were suspended in plasma and serum was removed from patients in septic shock. In addition, blocking monoclonal antibodies against the platelet receptor P-selectin and neutrophil receptor CD11b/CD18, and a platelet glycoprotein IIb/IIIa inhibitor, were incubated with cells suspended in plasma. Phase contrast video microscopy was used to count the number of neutrophils/mm adherent to endothelial cells during cessation of flow. Neutrophil rolling velocity was calculated as the time required for neutrophils to move across a 1-mm field (mm/sec). Leukoaggregation was defined as the number of neutrophils in aggregates (three or more nuclei) across a 1-mm field.
  
  
  RESULTS
  Normal neutrophils exposed to plasma from patients with septic shock demonstrated significant increases in aggregation and endothelial cell adherence with associated decreases in neutrophil rolling velocity. These changes were significantly enhanced in the presence of platelets and significantly attenuated in the presence of serum, which is fibrinogen depleted. Preincubation with antibodies to the surface receptors P-selectin, CD11b/CD18, and glycoprotein IIb/IIIa abrogated the changes in neutrophil aggregation, adhesion, and rolling velocity.
  
  
  CONCLUSIONS
  These data suggest that platelets and fibrinogen play an important role in mediating neutrophil-endothelial cell adherence in septic shock.
authors.affiliation: Department of Medicine, New York Medical College, Valhalla, New York, USA.
authors.forename: Linda A
authors.initials: LA
authors.lastname: Kirschenbaum
authors.forename: Dennis
authors.initials: D
authors.lastname: McKevitt
authors.forename: Melinda
authors.initials: M
authors.lastname: Rullan
authors.forename: Bernd
authors.initials: B
authors.lastname: Reisbeck
authors.forename: Tisha
authors.initials: T
authors.lastname: Fujii
authors.forename: Mark E
authors.initials: ME
authors.lastname: Astiz
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D016022:Case-Control Studies; D002448:Cell Adhesion / Q000502:physiology; D002449:Cell Aggregation / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D012772:Shock, Septic / Q000503:physiopathology*
chemical_list: D005340:Fibrinogen