title: Surface modified superparamagnetic nanoparticles for drug delivery: interaction studies with human fibroblasts in culture.
abstract: The concept of drug delivery using magnetic nanoparticles greatly benefit from the fact that nanotechnology has developed to a stage that it makes possible not only to produce magnetic nanoparticles in a very narrow size distribution range with superparamagnetic properties but also to engineer particle surfaces to provide site specific delivery of drugs. The size and surface characteristics of the nanoparticles are crucial factors that determine the success of the particles when used in vivo. The aim of this study was to modify the surfaces of the magnetic nanoparticles with PEG to improve the biocompatibility of the nanoparticles by resisting protein adsorption and increasing their intracellular uptake. In this study, the poly(ethyleneglycol) (PEG) modified superparamagnetic iron oxide nanoparticles have been prepared and their influence on human dermal fibroblasts is assessed in terms of cell adhesion/viability, morphology, particle uptake and cytoskeletal organisation studies. Various techniques have been used to determine nanoparticle-cell interactions including light, fluorescence, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The modification of nanoparticle surface induced alterations in cell behaviour distinct from the unmodified particles, suggesting that cell response can be directed via specifically engineered particle surfaces.
authors.affiliation: Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK. akgupta25@hotmail.com
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Gupta
authors.forename: Adam S G
authors.initials: AS
authors.lastname: Curtis
mesh_terms: D002448:Cell Adhesion; D048429:Cell Size; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000493:pharmacokinetics; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry* / Q000493:pharmacokinetics*; D052203:Ferrosoferric Oxide; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; C427127:superparamagnetic blood pool agent; D011092:Polyethylene Glycols; D052203:Ferrosoferric Oxide