Quantitative analysis of caffeic acid phenethyl ester in crude propolis by liquid chromatography-electrospray ionization mass spectrometry.
Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antinflammatory, and immunomodulatory properties. The paper describes a rapid and simple liquid chromatography-electrospray ionisation mass spectrometry method for qualitative and quantitative determination of CAPE. The chromatographic separation was performed with a Luna RP-C18 column using a water-acetonitrile linear gradient. The method was linear over a 0.125-80 ng/mL range (LOD = 62.5 pg/mL). The method was applied for the quantitation of caffeic acid phenethyl ester in crude propolis samples, which were analysed directly after extraction with ethyl acetate solution.
Del Boccio
Piero
P
Gennaro Paone Environmental Health Center, Mario Negri Institute for Pharmacological Research, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy.
Rotilio
Domenico
D
D002109:Caffeic Acids / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D003603:Cytotoxins / Q000302:isolation & purification*; D007202:Indicators and Reagents; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D011429:Propolis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D002109:Caffeic Acids; D003603:Cytotoxins; D007202:Indicators and Reagents; D011429:Propolis; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol