[Influence of vacuum-assisted closure technique on expression of Bcl-2 and NGF/NGFmRNA during wound healing].
OBJECTIVE
To explore the influence of vacuum-assisted closure technique (VAC) on expression of Bcl-2 and NGF during wound healing.


METHODS
Eighty Sprague-Dawley rats were randomly divided into 4 groups with 20 rats of each. Group T was the experimental group; group C1, C2 and C3 were the control groups. In group T and group C1, capsaicin was injected subcutaneously to the back of the rats to destroy the sensory nerve. VAC was employed to the wound of the rats in group T and C2 three times a day at 80 mmHg negative pressure. In all the groups, tissue samples were taken from the wound edge and granulation at 1, 3, 6, 9 and 12 days after the injury. Immunohistochemistry and in situ hybridization were used to detect the expression of Bcl-2 and NGF/NGFmRNA in the samples.


RESULTS
In group C2 and C3, the expression of Bcl-2 and NGF/NGFmRNA was obvious, which increased gradually and reached the peak at the 9th day. In the process of wound healing, the expression Bcl-2 and NGF/NGFmRNA was higher in the group C2 than in group C3 (P < 0.05). The expression Bcl-2 and NGF/NGFmRNA in group T and C1 was lower than group C2 and C3 (P < 0.05).


CONCLUSIONS
The application of the vacuum-assisted closure technique during wound healing increases the expression of the apoptosic modulation related protein Bcl-2 and affects the expression of NGF/NGFmRNA, which may promote the wound healing process.
Tang
Su-yang
SY
Department of Plastic Surgery, Tang Du Hospital, the Fourth Military Medical University, Xi'an 710038, China.
Chen
Shao-zong
SZ
Hu
Zhao-hua
ZH
Song
Mei
M
Cao
Da-yong
DY
Lü
Xiao-xing
XX
D000818:Animals; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D023421:Models, Animal; D020932:Nerve Growth Factor / Q000032:analysis / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014618:Vacuum; D014945:Wound Healing
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D020932:Nerve Growth Factor