title: Hematologic reconstitution following high-dose and supralethal chemoradiotherapy using stored, noncryopreserved autologous hematopoietic stem cells.
abstract: Although cryopreservation is the standard for autotransplantation, it has logistic and financial disadvantages in undeveloped countries such as Colombia. In 47 patients, peripheral blood was refrigerated at 4 degrees C up to 144 h before autotransplantation. For mobilization, 27 men and 20 women of median age 37 years affected with hematologic malignancies received G-CSF. The 17 patients in Group 1 showed pre-refrigeration CFU-GM of 2.62 x 10(5)/kg (range 0.36 to 16.6 x 10(5)/kg) and at re-infusion, 1.36 x 10(5)/kg (range 0 to 6.32 x 10(5)/kg) of 83% viability (range, 78% to 96%). These patients showed >0.5 x 10(9)/L granulocytes on day +11 (range, 9 to 15) and >20 x 10(9)/L platelets on day +16 (range, 11 to 44). The 25 patients in Group 2 showed CD34 of 3.9 x 10(6)/kg (range, 0.16 to 9 x 10(6)/kg) and mononuclear cell count (MNC) of 8.7 x 10(8)/kg, reaching >0.5 x 10(9)/L granulocytes at day +13 (range, 10 to 17) and >20 x 10(9)/L on day +15 (range, 14 to 20). Among the 5 patients in Group 3, the average of MNC of 12.7 x 10(8)/kg was reached and >0.5 x 10(9)/L granulocytes on day 11 (range, 10 to 16) and >20 x 10(9)/L on day 14 (range, 10 to 18). No differences were observed between the groups. Refrigeration of stem cells appears to be a simple, effective, and inexpensive method that should be considered for autotransplants within a few days of harvesting when resources are limited for long-term storage.
authors.affiliation: Blood and Marrow Transplantation Unit, Hospital San Vicente de Paul, Department of Hematology, School of Medicine, The University of Antioquia, Medellin, Colombia. fcuellar@epm.net.co
authors.forename: F
authors.initials: F
authors.lastname: Cuellar-Ambrosi
authors.forename: U A
authors.initials: UA
authors.lastname: Karduss
authors.forename: W R
authors.initials: WR
authors.lastname: Gomez
authors.forename: M C
authors.initials: MC
authors.lastname: Mondragon
authors.forename: M
authors.initials: M
authors.lastname: Velasquez-Lopera
authors.forename: S
authors.initials: S
authors.lastname: Calle
mesh_terms: D002470:Cell Survival; D003105:Colombia; D003114:Colony-Forming Units Assay; D004906:Erythrocyte Count; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D019337:Hematologic Neoplasms / Q000628:therapy*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count; D014021:Tissue Preservation / Q000379:methods; D014182:Transplantation, Autologous
chemical_list: D016179:Granulocyte Colony-Stimulating Factor