title: Restraint stress affects hippocampal cell proliferation differently in rats and mice.
abstract: Granule cell neurogenesis occurs in the dentate gyrus of the mammalian hippocampus throughout adult life, and incorporation of bromodeoxyuridine (BrdU) into DNA can serve as a marker of cell division associated with such neurogenesis. We examined the effects of a stressor (3h of restraint) on hippocampal cell proliferation in Sprague-Dawley rats and C57BL/6J mice. Animals were killed immediately following restraint stress and their brains were prepared for immunohistochemical studies. Restraint stress caused similar significant increases in c-Fos immunoreactivity among cells in the hypothalamic paraventricular nucleus of both species, indicating that the stress experienced was similar. The restraint procedure also caused a significant decrease in BrdU labeling in the dentate gyrus of rats, as previously reported, but a significant increase in the same region in mice. Hippocampal cell proliferation appears to respond differently to restraint stress in these species.
authors.affiliation: Department of Psychology, Life Sciences Centre, Dalhousie University, Halifax, NS, Canada B3H 4J1.
authors.forename: Megan J
authors.initials: MJ
authors.lastname: Bain
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Dwyer
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rusak
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D018891:Dentate Gyrus / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010286:Paraventricular Hypothalamic Nucleus / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D016196:S Phase; D013045:Species Specificity; D013315:Stress, Psychological / Q000473:pathology*
chemical_list: D000963:Antimetabolites; D016760:Proto-Oncogene Proteins c-fos; D001973:Bromodeoxyuridine