Identification of embryonic chromosomal abnormality using FISH-based preimplantation genetic diagnosis.
OBJECTIVE
Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF) failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF.


METHODS
Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF.


RESULTS
Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted, resulting in four clinical pregnancies, two of which led to successful normal birth of two healthy babies; one to ongoing pregnancy during the writing of this article; and one to ectopic pregnancy.


CONCLUSIONS
FISH-based PGD is an effective method for detecting embryonic chromosomal abnormality, which is one of the common causes of spontaneous miscarriages and chromosomally unbalanced offsprings.
Ye
Ying-hui
YH
Department of Reproductive Endocrinology, Affiliated Women's Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. yeyhcn2000@yahoo.com.cn
Xu
Chen-ming
CM
Jin
Fan
F
Qian
Yu-li
YL
D000328:Adult; D002869:Chromosome Aberrations / Q000196:embryology*; D025063:Chromosome Disorders / Q000175:diagnosis / Q000196:embryology* / Q000235:genetics*; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000235:genetics; D019836:Preimplantation Diagnosis / Q000379:methods*; D011379:Prognosis