Recognition of hepatotoxic homologues of Microcystin using a combination of selective adsorption media.
Microcystin, a hepatotoxin produced by cyanobacteria, was chromatographically discriminated with newly prepared selective adsorption media. Since the name Microcystin refers to up to 70 different homologues, we have developed a novel technique to prepare selective recognition sites, based on the "Fragment Imprinting Effect". The homologues of Microcystin can be individually discriminated from each other by antigen-antibody assay as well as by C18-based HPLC. This is mainly due to differences in the three dimensional structure of Microcystin homologues; however, differences in toxicity are relatively small among the homologues. Therefore we have to measure the total amount of Microcystin homologues present to determine contamination by Microcystins. We have demonstrated the first group recognition for Microcystin homologues through combination of two different selective adsorption media prepared by a fragment imprinting technique.
Kubo
Takuya
T
Department of Polymer Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Hosoya
Ken
K
Watabe
Yoshiyuki
Y
Tanaka
Nobuo
N
Sano
Tomoharu
T
Kaya
Kunimitsu
K
D001427:Bacterial Toxins / Q000737:chemistry* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000458:Cyanobacteria; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D052998:Microcystins; D008958:Models, Molecular; D008968:Molecular Conformation; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000633:toxicity
D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin