Effect of sodium nitroprusside on ischemia-reperfusion injuries of the rat liver.
OBJECTIVE
The role of NO (nitric oxide) in liver ischemia-reperfusion injury remains controversial, therefore the effect of sodium nitroprusside (SNP), as NO donor on ischemia-reperfusion injuries of the rat liver was studied.


METHODS
Warm ischemia (68% of total liver) of the rat liver was induced for 30 min, followed by reperfusion for 60 min. A dose of 1 or 10 microg/kg/min SNP were continuously administered during the reperfusion period. We performed the following assessments: (i) hepatic microcirculation, (ii) nitrosyl-hemoglobin (Hb-NO, a product of interactions between hemoglobin and NO) levels, (iii) NO2-/NO3- (end products of NO oxidation) levels, and (iv) the serum lactate-dehydrogenase (LDH) levels.


RESULTS
Hepatic microcirculation at 5 and 15 min after start of SNP infusion was significantly improved, compared with that in the control group. NO2-/NO3- and Hb-NO levels in both SNP groups were gradually elevated after reperfusion. Serum LDH levels at 60 min after start of reperfusion in both SNP groups were significantly lower than in the control group.


CONCLUSIONS
In the early phase of the reperfusion period, SNP injected increased hepatic microcirculation and reduced hepatocyte damage, suggesting that the administration of SNP may help present ischemia-reperfusion injuries.
Kuroki
Ikyoko
I
Department Anesthesiology, Saitama Medical School, Saitama, Japan.
Miyazaki
Takashi
T
Mizukami
Isatoshi
I
Matsumoto
Nobuyuki
N
Matsumoto
Isao
I
D000818:Animals; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000503:physiopathology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*
D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase