[Effects of different activation methods on in vitro development of bovine somatic cloned embryos].
Effects of different activation methods on the cleavage and in vitro development of bovine somatic cloned embryos constructed by intracytoplasmic nuclear injection were compared. The results show that the cleavage and in vitro development rate were not different significantly for constructed embryos cultured in 6-DMAP comparing with those in 6-DMAP + cytochalasin B (CCB) after activation with Ionomycin. Culture duration (3 to 4 h) in 6-DMAP or 6-DMAP + CCB had no significant effects on the cleavage and in vitro development ability of reconstructed embryos. CCB addition in the activation medium was benefit to the development of constructed embryos, although the effect wasn't significant. Within 1 to 4 h, the longer interval duration of nuclear injection and reconstructed embryo activation was, the higher cleavage and the blastocyst development rate of reconstructed embryos were.
Li
Xue Feng
XF
College of Life Science, South China Normal University, Guangzhou 510631.
Li
Yu
Y
Guo
Ji Tong
JT
An
Zhi Xin
ZX
Zhang
Xiu
X
Zhang
Yong
Y
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003571:Cytochalasin B / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods*; D047108:Embryonic Development / Q000187:drug effects*; D015759:Ionomycin / Q000494:pharmacology*; D020554:Sperm Injections, Intracytoplasmic
D003571:Cytochalasin B; D015759:Ionomycin; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine