title: The effects of zinc deficiency and supplementation on lipid peroxidation in bone tissue of ovariectomized rats.
abstract: This study aims at investigating how zinc deficiency and zinc application affect lipid peroxidation in bone tissue of ovariectomized rats. The study included 40 adult female rats of Sprague-Dawley species. Group 1 (n = 10): control group were fed with normal diet. Group 2 (n = 10): the group fed with normal diet after being ovariectomized. Group 3 (n = 10): the group fed with zinc-deficient diet for 6 weeks after ovariectomy. Group 4 (n = 10): the group which was given intraperitoneal zinc (3 mg/kg day zinc) in addition to normal diet for 6 weeks after ovariectomy. Malondialdehyde (MDA) and glutathione (GSH) levels were determined in erythrocyte, plasma and bone tissue. Group 3 had the highest plasma MDA levels compared to Groups 1, 2 and 4 (P < 0.05). These values were higher in Group 2 than in Groups 1 and 4 (P < 0.05). Bone and plasma MDA levels in Groups 1 and 4 were not different. Bone and erythrocyte GSH levels in Group 4 were higher than those in all other groups (P < 0.05). The lowest levels of bone and erythrocyte GSH levels were observed in Group 3 (P < 0.05). These values were higher in Group 2 when compared to those in Groups 1 and 3 (P < 0.05). This study demonstrate that zinc deficiency increased bone tissue damage in ovariectomized rats and that zinc supplementation prevented this damage.
authors.affiliation: Department of Physiology, Meram Medical School, Selcuk University, 42080 Konya, Turkey. baltaci@selcuk.edu.tr
authors.forename: Abdulkerim Kasim
authors.initials: AK
authors.lastname: Baltaci
authors.forename: Fusun
authors.initials: F
authors.lastname: Sunar
authors.forename: Rasim
authors.initials: R
authors.lastname: Mogulkoc
authors.forename: Esma
authors.initials: E
authors.lastname: Oztekin
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D004032:Diet; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology*
chemical_list: D008315:Malondialdehyde; D005978:Glutathione; D015032:Zinc