[Re-growth of induced-osseous tissue encapsulated in muscular fasciae for prefabrication of an osteo-musculo-cutaneous flap].
OBJECTIVE
To improve the survival of mesenchymal stem cells (MSCs) for prefabrication of an osteo-musculo-cutaneous flap.


METHODS
In a mouse model, the compound of MSCs and p(3HB-co-3HH) were embeded in the latissimus dorsi muscle as an experimental group and the muscle pocket of the buttock as the control. The examinations of the HE staining, hybridization in situ of osteonectin mRNA and Von kossa staining were used to evaluate the results.


RESULTS
The expression of osteonectin mRNA and the Von kossa staining showed that the latissimus dorsi muscle group was superior to the control in 2 weeks and 4 weeks after the surgery in vivo.


CONCLUSIONS
The results indicate that the above-mentioned technique may be a good alternative for the prefabrication of the osteo-musculo-cutaneous flap.
Zhao
Yu
Y
Surgery Hospital Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100041, China.
Hu
Ping
P
Lu
Ying-ling
YL
Gao
Feng
F
Qiao
Qun
Q
Qi
Ke-ming
KM
D000818:Animals; D005205:Fascia / Q000637:transplantation*; D006651:Histocytochemistry / Q000379:methods; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D033581:Stem Cell Transplantation / Q000379:methods; D013524:Surgical Flaps / Q000098:blood supply*; D023822:Tissue Engineering / Q000379:methods*
D015676:Osteonectin; D012333:RNA, Messenger