title: Injury-induced alterations in N-methyl-D-aspartate receptor subunit composition contribute to prolonged 45calcium accumulation following lateral fluid percussion.
abstract: Cells that survive traumatic brain injury are exposed to changes in their neurochemical environment. One of these changes is a prolonged (48 h) uptake of calcium which, by itself, is not lethal. The N-methyl-D-aspartate receptor (NMDAR) is responsible for the acute membrane flux of calcium following trauma; however, it is unclear if it is involved in a flux lasting 2 days. We proposed that traumatic brain injury induced a molecular change in the NMDAR by modifying the concentrations of its corresponding subunits (NR1 and NR2). Changing these subunits could result in a receptor being more sensitive to glutamate and prolong its opening, thereby exposing cells to a sustained flux of calcium. To test this hypothesis, adult rats were subjected to a lateral fluid percussion brain injury and the NR1, NR2A and NR2B subunits measured within different regions. Although little change was seen in NR1, both NR2 subunits decreased nearly 50% compared with controls, particularly within the ipsilateral cerebral cortex. This decrease was sustained for 4 days with levels returning to control values by 2 weeks. However, this decrease was not the same for both subunits, resulting in a decrease (over 30%) in the NR2A:NR2B ratio indicating that the NMDAR had temporarily become more sensitive to glutamate and would remain open longer once activated. Combining these regional and temporal findings with 45calcium autoradiographic studies revealed that the degree of change in the subunit ratio corresponded to the extent of calcium accumulation. Finally, utilizing a combination of NMDAR and NR2B-specific antagonists it was determined that as much at 85% of the long term NMDAR-mediated calcium flux occurs through receptors whose subunits favor the NR2B subunit. These data indicate that TBI induces molecular changes within the NMDAR, contributing to the cells' post-injury vulnerability to glutamatergic stimulation.
authors.affiliation: Brain Injury Research Center, Division of Neurosurgery/Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-7039, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Osteen
authors.forename: C C
authors.initials: CC
authors.lastname: Giza
authors.forename: D A
authors.initials: DA
authors.lastname: Hovda
mesh_terms: D000818:Animals; D001345:Autoradiography; D001522:Behavior, Animal / Q000187:drug effects; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000523:psychology; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D009778:Occipital Lobe / Q000378:metabolism; D009930:Organic Chemicals; D010296:Parietal Lobe / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013997:Time Factors; D015599:Trauma Severity Indices; D014949:Wounds, Nonpenetrating / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology / Q000523:psychology
chemical_list: D002132:Calcium Radioisotopes; D018691:Excitatory Amino Acid Antagonists; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D010880:Piperidines; D020033:Protein Isoforms; D016194:Receptors, N-Methyl-D-Aspartate; C435731:fluoro jade; D016291:Dizocilpine Maleate; C010739:ifenprodil; D002118:Calcium