Shedding of mutant tumor necrosis factor receptor superfamily 1A associated with tumor necrosis factor receptor-associated periodic syndrome: differences between cell types.
OBJECTIVE
To investigate the effect of mutations in tumor necrosis factor receptor superfamily 1A (TNFRSF1A) on the ability of the receptors to be cleaved from the cell surface upon stimulation. The mutations we studied are associated with clinically distinct forms of TNF receptor-associated periodic syndrome (TRAPS). We also investigated different cell types within the same form of TRAPS.


METHODS
The shedding of TNFRSF1A in response to stimulation with phorbol myristate acetate was assessed in leukocytes and dermal fibroblasts from patients with C33Y TRAPS, and in HEK 293 cell lines stably transfected with constructs containing wild-type TNFRSF1A and/or TNFRSF1A mutants identified in TRAPS patients.


RESULTS
The shedding of TNFRSF1A differed between cell types within the same form of TRAPS. In particular, dermal fibroblasts, but not leukocytes, from C33Y TRAPS patients demonstrated reduced shedding of TNFRSF1A. Shedding of both wild-type and mutant TNFRSF1A from the transfected HEK 293 cells showed minor differences, but was in all cases induced to a substantial extent.


CONCLUSIONS
Differences in TNFRSF1A shedding are not purely a function of the TNFRSF1A structure, but are also influenced by other features of genetic makeup and/or cellular differentiation. It is unlikely that a defect in TNFRSF1A shedding per se can fully explain the clinical features that are common to TRAPS patients with different TNFRSF1A mutations.
Huggins
Mary L
ML
Division of Immunology, Queen's Medical Centre, University of Nottingham, Nottingham, UK. mary.huggins@nottingham.ac.uk
Radford
Paul M
PM
McIntosh
Richard S
RS
Bainbridge
Susan E
SE
Dickinson
Peter
P
Draper-Morgan
Kelly-Ann
KA
Tighe
Patrick J
PJ
Powell
Richard J
RJ
Todd
Ian
I
D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D010505:Familial Mediterranean Fever / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D009154:Mutation; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics* / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015703:Antigens, CD; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D013755:Tetradecanoylphorbol Acetate