title: Stobadine-modulated human neutrophil functional responsiveness: effect on particular and soluble stimulation.
abstract: The effect of stobadine on degranulation (myeloperoxidase release) and on oxidative burst, measured as superoxide anion production, was investigated in human neutrophils activated with receptor-specific (fMLP, opsonized zymosan) and nonreceptor stimuli (PMA, A 23187). Wortmannin, a specific inhibitor of 1-phosphatidylinositol 3-kinase, significantly inhibited fMLP-stimulated generation only. This effect was pronounced by stobadine. Stobadine dose-dependently decreased superoxide generation and myeloperoxidase release after receptor-specific stimuli, with the highest effect on fMLP stimulation of superoxide generation and on opsonized zymosan stimulation of myeloperoxidase release.
authors.affiliation: Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic. exfajape@savba.sk
authors.forename: J
authors.initials: J
authors.lastname: Pecivová
authors.forename: T
authors.initials: T
authors.lastname: Macicková
authors.forename: R
authors.initials: R
authors.lastname: Nosál'
authors.forename: V
authors.initials: V
authors.lastname: Fabryová
mesh_terms: D002243:Carbolines / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology; D012995:Solubility / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism
chemical_list: D002243:Carbolines; D005609:Free Radicals; D013481:Superoxides; C100106:dicarbine