[In silico cloning of evolutionarily conserved mouse F-LANa].
Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. In previous study, human F-LANa was identified as a differentially expressed gene, up-regulated in hepatocellular carcinoma. In this study, we developed an in silico cloning approach to rapidly and accurately characterize the mouse ortholog of the human F-LANa. Mouse F-LANa encodes a 239 aa protein exhibiting 97.9% similarity to the human ortholog gene. Homology analysis was carried out in various species and showed that F-LANa was evolutionarily conserved from yeast to human. Based on the alignment results, phylogenetic tree was established here.
Ying
Hao
H
Xu
Yong Hua
YH
D000818:Animals; D015139:Blotting, Southern; D045744:Cell Line, Tumor; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
C513304:Derl2 protein, mouse; D008565:Membrane Proteins