title: Characterization of the human salivary basic proline-rich protein complex by a proteomic approach.
abstract: Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8c), P-H (or IB-4), IB-6, II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRPs (apart from the P-B peptide) which is in agreement with their common parotid origin.
authors.affiliation: Department of Sciences Applied to Biosystems, Cagliari University, Cagliari, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cabras
authors.forename: Rosanna
authors.initials: R
authors.lastname: Inzitari
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lupi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zuppi
authors.forename: Chiara
authors.initials: C
authors.lastname: Olmi
authors.forename: Maria Benedetta
authors.initials: MB
authors.lastname: Fadda
authors.forename: Massimo
authors.initials: M
authors.lastname: Cordaro
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D055232:Proline-Rich Protein Domains; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*
chemical_list: D010455:Peptides