title: Temporal global changes in gene expression during temperature transition in Yersinia pestis.
abstract: DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open reading frames (ORFs) on pCD, pPCP, and pMT were thermoinduced and that the majority of these genes carried by pCD were downregulated by Ca2+. In contrast, Ca2+ had little effect on chromosomal genes and ORFs, of which 235 were thermally upregulated and 274 were thermally downregulated. The primary consequence of these regulatory events is profligate catabolism of numerous metabolites available in the mammalian host.
authors.affiliation: Biology and Biotechnology Research Program, L-452, 7000 East Ave., Livermore, CA 94550, USA.
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Motin
authors.forename: Anca M
authors.initials: AM
authors.lastname: Georgescu
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Fitch
authors.forename: Pauline P
authors.initials: PP
authors.lastname: Gu
authors.forename: David O
authors.initials: DO
authors.lastname: Nelson
authors.forename: Shalini L
authors.initials: SL
authors.lastname: Mabery
authors.forename: Janine B
authors.initials: JB
authors.lastname: Garnham
authors.forename: Bahrad A
authors.initials: BA
authors.lastname: Sokhansanj
authors.forename: Linda L
authors.initials: LL
authors.lastname: Ott
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Coleman
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Elliott
authors.forename: Laura M
authors.initials: LM
authors.lastname: Kegelmeyer
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wyrobek
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Slezak
authors.forename: Robert R
authors.initials: RR
authors.lastname: Brubaker
authors.forename: Emilio
authors.initials: E
authors.lastname: Garcia
mesh_terms: D000222:Adaptation, Physiological; D002118:Calcium / Q000378:metabolism; D002876:Chromosomes, Bacterial; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids; D013696:Temperature; D015010:Yersinia pestis / Q000235:genetics*
chemical_list: D002118:Calcium