title: A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives.
abstract: A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Jiyou
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Liu
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
authors.forename: Zhide
authors.initials: Z
authors.lastname: Hu
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007834:Lasers; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000596:Amino Acids; D009327:4-Chloro-7-nitrobenzofurazan