[Effect of melittin on apoptosis and necrosis of U2 OS cells].
OBJECTIVE
To study the effect of melittin on apoptsis and necrosis of osteosarcoma cell line U2 OS in vitro.


METHODS
Osteosarcoma cell line U2 OS was treated with melittin. The growth and proliferation was observed by MTT assay and cell counting, and the necrosis was estimated by Trypan blue staining. The cell apoptsis, Fas and Apo2. 7 expression were detected by cytometer.


RESULTS
The data showed that melittin could inhibit the proliferation of U2 OS dose-dependently at 16 and 64 mg/L. Cell apoptsis was detected by cytometer, when the cells were treated by 16 mg/L and 32 mg/L of melittin respectively, and the percentages of Fas and Apo2. 7 positive cells were increased.


CONCLUSIONS
Melittin inhibits the proliferation of osterosarcoma cell line through up-regulating Fas expression and inducing apoptsis.
Chen
Yong-Qiang
YQ
Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. YQCHEN116@hotmail.com
Zhu
Zhen-An
ZA
Hao
Yong-Qiang
YQ
Dai
Ke-Rong
KR
Zhang
Chen
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D020128:Inhibitory Concentration 50; D008555:Melitten / Q000633:toxicity*; D010020:Osteonecrosis / Q000139:chemically induced / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D019014:fas Receptor
C491901:FAS protein, human; D011518:Proto-Oncogene Proteins; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; D008555:Melitten