Preparation of primary amine-modified gold nanoparticles and their transfection ability into cultivated cells.
Cationic gold nanoparticles, prepared by reducing HAuCl(4) with NaBH(4) in the presence of 2-aminoethanethiol, formed a complex with plasmid DNA, and could be transfected into cultivated cells.
Niidome
Takuro
T
Department of Materials Science, Graduate School of Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan. niidome-tcm@mbox.nc.kyushu-u.ac.jp
Nakashima
Kanako
K
Takahashi
Hironobu
H
Niidome
Yasuro
Y
D000588:Amines / Q000737:chemistry*; D000818:Animals; D001894:Borohydrides / Q000737:chemistry; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003543:Cysteamine / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D006046:Gold / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
D000588:Amines; D001894:Borohydrides; D003543:Cysteamine; D006046:Gold; C025364:sodium borohydride; D004247:DNA