Characterization of polyacrylamide based monolithic columns.
Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker N,N'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like structure with interconnected supermacropores of pore size 5-100 microm. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column-to-column reproducibility of pAAm-monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same cryostructuration conditions.
Plieva
Fatima M
FM
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden.
Andersson
Jonatan
J
Galaev
Igor Yu
IY
Mattiasson
Bo
B
D000096:Acetone / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D003300:Copper / Q000737:chemistry; D059985:Cryogels; D004852:Epoxy Compounds / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D005615:Freezing; D020100:Hydrogels; D007477:Ions; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D011312:Pressure; D013696:Temperature; D013997:Time Factors; D000099166:Polyacrylamides
D000096:Acetone; D000178:Acrylamides; D000180:Acrylic Resins; D001798:Blood Proteins; D003300:Copper; D059985:Cryogels; D004852:Epoxy Compounds; D005353:Fibronectins; D020100:Hydrogels; D007477:Ions; D011108:Polymers; D000099166:Polyacrylamides; C021221:N,N'-methylenebisacrylamide; C054361:allyl glycidyl ether