Molecular diagnosis of leukemic cerebrospinal fluid cells in children with newly diagnosed acute lymphoblastic leukemia.
Cytomorphology and IgH/T-cell receptor g clonal gene rearrangements detected by polymerase chain reaction (PCR) homo/heteroduplex analysis and direct sequencing were evaluated in cerebrospinal fluid (CSF) free of red-blood cells at diagnosis of 37 children with acute lymphoblastic leukemia. Molecular CSF involvement was greater as detected by molecular analysis than observed by morphologic criteria (45.9% vs 5.4%). The 4-year event-free survival was lower in the group with molecularly detected CSF involvement (p = 0.01).
Scrideli
Carlos A
CA
Queiroz
Rosane P
RP
Takayanagui
Osvaldo M
OM
Bernardes
Jose E
JE
Melo
Enaldo V
EV
Tone
Luiz G
LG
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000134:cerebrospinal fluid; D018572:Disease-Free Survival; D006801:Humans; D016130:Immunophenotyping; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000235:genetics*; D013997:Time Factors
D004247:DNA