title: [The influence of temperature on the protein expression of human lung cancer cell line A549].
abstract: The study on proteome of human cancer is helpful to explain its pathogenesis and make good effect on its prevention and cure. We compared the 2-DE maps of whole proteins of human lung cancer line A549 at 37 degrees C, 42 degrees C and 45 degrees C for the purpose of studying the expression of its heat shock proteins. Three temperature-sensitive differential spots were obtained and named as P1, P2, P3, respectively. Analyzed by MALDI-TOF-MS and Peptident software searched in the SWISS-PROT database, the three differential proteins were elementarily identified. P1 matches with two proteins belong to the Aldo-keto reductase family, P2 may be a new protein and P3 is Zinc finger protein 11A.
authors.affiliation: The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005.
authors.forename: Zi Jun
authors.initials: ZJ
authors.lastname: Chen
authors.forename: San Ying
authors.initials: SY
authors.lastname: Wang
authors.forename: Jing An
authors.initials: JA
authors.lastname: Chen
authors.forename: Xuan Xian
authors.initials: XX
authors.lastname: Peng
mesh_terms: D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins