title: Inhibitory effects of berberine on ion channels of rat hepatocytes.
abstract: OBJECTIVE
  To examine the effects of berberine, an isoquinoline alkaloid with a long history used as a tonic remedy for liver and heart, on ion channels of isolated rat hepatocytes.
  
  
  METHODS
  Tight-seal whole-cell patch-clamp techniques were performed to investigate the effects of berberine on the delayed outward potassium currents (I(K)), inward rectifier potassium currents (I(K1)) and Ca(2+) release-activated Ca(2+) currents (I(CRAC)) in enzymatically isolated rat hepatocytes.
  
  
  RESULTS
  Berberine 1-300 micromol/L reduced I(K) in a concentration-dependent manner with EC(50) of 38.86+/-5.37 micromol/L and n(H) of 0.82+/-0.05 (n = 8). When the bath solution was changed to tetraethylammonium (TEA) 8 mmol/L, I(K) was inhibited. Berberine 30 micromol/L reduced I(K) at all examined membrane potentials, especially at potentials positive to +60 mV (n = 8, P<0.05 or P<0.01 vs control). Berberine had mild inhibitory effects on I(K1) in rat hepatocytes. Berberine 1-300 micromol/L also inhibited I(CRAC) in a concentration-dependent fashion. The fitting parameters were EC(50) = 47.20+/-10.86 micromol/L, n(H) = 0.71+/-0.09 (n = 8). The peak value of I(CRAC) in the I-V relationship was decreased by berberine 30 micromol/L at potential negative to -80 mV (n = 8, P<0.05 vs control). But the reverse potential of I(CRAC) occurred at voltage 0 mV in all cells.
  
  
  CONCLUSIONS
  Berberine has inhibitory effects on potassium and calcium currents in isolated rat hepatocytes, which may be involved in hepatoprotection.
authors.affiliation: Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. wangfang0322@yahoo.com.cn
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Fu
authors.forename: Lan
authors.initials: L
authors.lastname: Cheng
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Yao
mesh_terms: D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007473:Ion Channels; D001599:Berberine