title: Characterization of the Arabidopsis TU8 glucosinolate mutation, an allele of TERMINAL FLOWER2.
abstract: Glucosinolates are a group of defense-related secondary metabolites found in Arabidopsis and other cruciferous plants. Levels of leaf glucosinolates are regulated during plant development and increase in response to mechanical damage or insect feeding. The Arabidopsis TU8 mutant has a developmentally altered leaf glucosinolate profile: aliphatic glucosinolate levels drop off more rapidly, consistent with the early senescence of the mutant, and the levels of two indole glucosinolates are uniformly low. In TU8 seeds, four long-chain aliphatic glucosinolates have significantly increased levels, whereas the indolyl-3-methyl glucosinolate level is significantly reduced relative to wild type. Genetic mapping and DNA sequencing identified the TU8 mutation as tfl2-6, a new allele of TERMINAL FLOWER2 (TFL2), the only Arabidopsis homolog of animal HETEROCHROMATIN PROTEIN1 (HP1). TU8 (tfl2-6) has other previously identified tfl2 phenotypes, including an early transition to flowering, altered meristem structure, and stunted leaves. Analysis of two additional alleles, tfl2-1 and tfl2-2, showed glucosinolate profiles similar to those of line TU8 (tfl2-6).
authors.affiliation: Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA.
authors.forename: Jae Hak
authors.initials: JH
authors.lastname: Kim
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Durrett
authors.forename: Robert L
authors.initials: RL
authors.lastname: Last
authors.forename: Georg
authors.initials: G
authors.lastname: Jander
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005961:Glucosinolates / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; D018744:DNA, Plant; D005961:Glucosinolates; C477788:At5g17690 protein, Arabidopsis