Inhibition by tranilast of nifedipine-induced proliferation of cultured human gingival fibroblasts.
The appropriate method of etiologic therapy for gingival overgrowth is yet unknown. In this study drug-induced proliferation of Gin-1 cells, a normal human gingival fibroblast cell line, was examined by using the reagent water-soluble tetrazolium-1. Tranilast (100 microM) inhibited the nifedipine (10 microM)-induced proliferation of gingival fibroblasts. The level of basic fibroblast growth factor (bFGF) was determined by using an enzyme-linked immunosorbent assay kit. Tranilast inhibited the release of bFGF from the cells. In conclusion, tranilast depresses the nifedipine-induced proliferation of gingival fibroblasts by inhibiting the release of bFGF. Administration of tranilast may thus be clinically effective for the treatment of gingival overgrowth.
Hattori
Toshimi
T
Department of Dental Pharmacology, Matsumoto Dental University, Shiojiri 399-0781, Japan. hattori@po.mdu.ac.jp
Wang
Pao-Li
PL
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006801:Humans; D009543:Nifedipine / Q000494:pharmacology*; D013997:Time Factors; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D062367:ortho-Aminobenzoates; D016222:Fibroblast Growth Factor 2; C012293:tranilast; D009543:Nifedipine