[Construction of a genechip method for detection of hepatitis B virus DNA, basal core promotor and Pre-C mutants].
BACKGROUND
To establish a genechip method for detection of hepatitis B virus (HBV) DNA, basal core promotor (BCP), and Pre-C mutants.


METHODS
This study used two kinds of technology (PCR, oligochip), which can detect five mutant hotspots including nt 1 896, nt 1 899, nt 1 862, nt 1 764 and nt 1 762. The results of genechip method was verified by DNA sequencing.


RESULTS
In detecting HBV DNA, the results of genechip were 100% consistent with those of DNA sequencing. In detecting HBV BCP and Pre-C mutants, 146 codons showed the same results using both methods, except for only 4 codons (P greater than 0.05).


CONCLUSIONS
This convenient high throughput genechip method could detect several BCP and Pre-C mutant codons at the same time. These results suggest that genechip method has the same positive rate and specificity with DNA sequencing method. It has more advantages than the latter in detecting mixed mutants and therefore may be used in clinical practice.
Li
Bing
B
Shenzhen Eastlake Hospital, Shenzhen 518020, China.
Peng
Jin-fu
JF
Chen
Li-yan
LY
Wang
Zhao-qin
ZQ
Li
Li-xiong
LX
Wang
Min
M
Li
Jiang
J
Zhou
Bo-ping
BP
D004279:DNA, Viral / Q000032:analysis*; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000235:genetics*
D004279:DNA, Viral; D014758:Viral Core Proteins