Application of peptide LC retention time information in a discriminant function for peptide identification by tandem mass spectrometry.
We describe the application of a peptide retention time reversed phase liquid chromatography (RPLC) prediction model previously reported (Petritis et al. Anal. Chem. 2003, 75, 1039) for improved peptide identification. The model uses peptide sequence information to generate a theoretical (predicted) elution time that can be compared with the observed elution time. Using data from a set of known proteins, the retention time parameter was incorporated into a discriminant function for use with tandem mass spectrometry (MS/MS) data analyzed with the peptide/protein identification program SEQUEST. For singly charged ions, the number of confident identifications increased by 12% when the elution time metric is included compared to when mass spectral data is the sole source of information in the context of a Drosophila melanogaster database. A 3-4% improvement was obtained for doubly and triply charged ions for the same biological system. Application to the larger Rattus norvegicus (rat) and human proteome databases resulted in an 8-9% overall increase in the number of confident identifications, when both the discriminant function and elution time are used. The effect of adding "runner-up" hits (peptide matches that are not the highest scoring for a spectra) from SEQUEST is also explored, and we find that the number of confident identifications is further increased by 1% when these hits are also considered. Finally, application of the discriminant functions derived in this work with approximately 2.2 million spectra from over three hundred LC-MS/MS analyses of peptides from human plasma protein resulted in a 16% increase in confident peptide identifications (9022 vs 7779) using elution time information. Further improvements from the use of elution time information can be expected as both the experimental control of elution time reproducibility and the predictive capability are improved.
Strittmatter
Eric F
EF
Biological Sciences Division and Environmental and Molecular Sciences Laboratory, Richland, Washington 99352, USA.
Kangas
Lars J
LJ
Petritis
Konstantinos
K
Mottaz
Heather M
HM
Anderson
Gordon A
GA
Shen
Yufeng
Y
Jacobs
Jon M
JM
Camp
David G
DG
Smith
Richard D
RD
D000818:Animals; D002853:Chromatography, Liquid; D029721:Drosophila Proteins / Q000737:chemistry; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D020543:Proteome / Q000737:chemistry*; D051381:Rats; D012984:Software
D029721:Drosophila Proteins; D010455:Peptides; D020543:Proteome