title: Recombinant bivalent vaccine against foot-and-mouth disease virus serotype O/A infection in guinea pig.
abstract: In this study, two DNA fragments encoding amino acid (141-160)-(21-140)-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.
authors.affiliation: State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai, China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Yi
authors.forename: Ming-Qiu
authors.initials: MQ
authors.lastname: Liu
authors.forename: Cai-Zhu
authors.initials: CZ
authors.lastname: Zhu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zu-Tian
authors.initials: ZT
authors.lastname: Sheng
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Du
authors.forename: Wei-Yao
authors.initials: WY
authors.lastname: Yan
authors.forename: Zhao-Xin
authors.initials: ZX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000939:Epitopes; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology*; D006168:Guinea Pigs; D012703:Serotyping; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines