title: In vitro cytotoxicity of E-glass fiber weave preimpregnated with novel biopolymer.
abstract: The aim of this study was to investigate cytotoxicity of composition of E-glass fibers and novel biopolymer of poly(hydroxyproline). Growth and proliferation of the human gingival fibroblast cells on the surface of the materials was evaluated. The number of cells grown and proliferated on cell culture plastic was used as a control. Bi-directional fiber weaves were preimpregnated with poly(hydroxyproline). Cytotoxicities of the preimpregnated and nonimpregnated materials were evaluated bythe release of lactate dehydrogenase from the cells during the culture period of 24 h. The values of the lactate dehydrogenase activity of the materials' extracts showed non-toxicity for poly(hydroxyproline) preimpregnated E-glass fiber weaves. The growth of fibroblasts on the surface of the materials appeared normal after 11 days culture period; they looked healthy and normal in size and shape. The results of this study suggest that based on its' non-cytotoxicity the composition of E-glass fibers and poly(hydroxyproline) can further be evaluated as a material that is suitable for biomedical use.
authors.affiliation: Turku Centre for Biomaterials, Institute of Dentistry, University of Turku, Itäinen Pitkäkatu 4B, FIN 20520 Turku, Finland. marju.vakiparta@utu.fi
authors.forename: M
authors.initials: M
authors.lastname: Väkiparta
authors.forename: M K
authors.initials: MK
authors.lastname: Koskinen
authors.forename: P
authors.initials: P
authors.lastname: Vallittu
authors.forename: T
authors.initials: T
authors.lastname: Närhi
authors.forename: A
authors.initials: A
authors.lastname: Yli-Urpo
mesh_terms: D001672:Biocompatible Materials / Q000633:toxicity*; D001704:Biopolymers / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing; D010455:Peptides / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D010455:Peptides; C009753:fiberglass; D007770:L-Lactate Dehydrogenase