In vivo multiphoton fluorescence imaging: a novel approach to oral malignancy.
OBJECTIVE
Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality.


METHODS
In the hamster model (n = 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM- and histopathology-based diagnoses on a scale of 0 (healthy)-6 (squamous cell carcinoma [s.c.c.]) were scored by two pre-standardized investigators.


RESULTS
Collagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses.


CONCLUSIONS
In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics.
Wilder-Smith
Petra
P
Beckman Laser Institute, University of California, Irvine, California 92612, USA. pwsmith@bli.uci.edu
Osann
Kathryn
K
Hanna
Nevine
N
El Abbadi
Naglaa
N
Brenner
Matt
M
Messadi
Diana
D
Krasieva
Tatiana
T
D000818:Animals; D006224:Cricetinae; D036641:Microscopy, Fluorescence, Multiphoton; D009062:Mouth Neoplasms / Q000473:pathology*