[Studies on calcium sensitive period during floral differentiation of cucumber cotyledonary node cultures in vitro].
Cotyledonary nodes of cucumber cultured on calcium-free medium for 0, 1, 2, 3, 4, 5, 6d respectively, were transferred to medium with 6.0 mmol/L CaCl2 for 24h, then returned to calcium-free medium. Cotyledonary nodes cultured on calcium-free or 6.0 mmol/L CaCl2 medium for all time, were taken as controls. Results showed that cotyledonary nodes were transferred to 6.0 mmol/L CaCl2 medium for 24h during 0-3d after the beginning of culture, percentage of floral bud formation at cotyledonary nodes was increased significantly. Transferring cotyledonary nodes on the 3d day after the beginning of culture was achieved best effect, percentage of floral bud formation was up to 34.3%. We deduced that the calcium sensitive period during floral differentiation of cucumber cotyleddonary node cultured in vitro may be 0-4d after the beginning of culture.
Wang
Li Lin
LL
School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
Pang
Ji Liang
JL
Hu
Jiang Qin
JQ
Zhang
Yan Yoan
YY
Liang
Hai Man
HM
D002122:Calcium Chloride / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D018548:Cotyledon / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D018553:Cucumis sativus / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D035264:Flowers / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development
D002122:Calcium Chloride