LC/MS for the degradation profiling of cough-cold products under forced conditions.
Heat, acid, base, UV radiation and oxidation stress methods were applied to study the stability of cough-cold products containing acetaminophen, phenylephrine or phenylpropanolamine hydrochloride and chlorpheniramine maleate. Liquid chromatography coupled with mass spectrometry was used to analyze the degraded samples and obtain molecular weights information. Different volatile buffers (ammonium bicarbonate and ammonium acetate) were assayed in LC/MS methods and retention times of the analytes were compared with those obtained in HPLC with UV detection employing a conventional sodium phosphate buffer to establish the possibility of results transference between the two systems.
Marín
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Facultad de CC Experimentales y de la Salud, Universidad San Pablo-CEU, Urbanización Montepríncipe, Boadilla del Monte, 28668 Madrid, Spain.
Barbas
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C
D000082:Acetaminophen / Q000737:chemistry; D002744:Chlorpheniramine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D004338:Drug Combinations; D004355:Drug Stability; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010656:Phenylephrine / Q000737:chemistry; D010665:Phenylpropanolamine / Q000737:chemistry; D015203:Reproducibility of Results; D019141:Respiratory System Agents / Q000737:chemistry*; D012680:Sensitivity and Specificity
D004338:Drug Combinations; D019141:Respiratory System Agents; D010656:Phenylephrine; D010665:Phenylpropanolamine; D000082:Acetaminophen; D002744:Chlorpheniramine