Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.
In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith Flash RP-18e, 25-4.6mm column (Merck, Germany) and a FIAlab 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10:90, v/v) pH 4.05, flow rate 0.6 mL min(-1). UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3-0.8 microg mL(-1), repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was <6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.
Satínský
Dalibor
D
The Research Centre LN00B125, Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského, Hradec Králové, Czech Republic. satinsky@faf.cuni.cz
Neto
Isabel
I
Solich
Petr
P
Sklenákova
Hana
H
Conceição
M
M
Montenegro
B S M
BS
Araújo
Alberto N
AN
D000082:Acetaminophen / Q000032:analysis*; D001241:Aspirin / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets / Q000737:chemistry*
D007202:Indicators and Reagents; D012997:Solvents; D013607:Tablets; D000082:Acetaminophen; D002110:Caffeine; D001241:Aspirin