Identification of the critical cis-acting elements in the promoter of the mouse Prnd gene coding for Doppel protein.
Prnd gene encodes a prion-like protein Doppel. To elucidate the transcriptional regulation of the mouse Prnd gene, we performed a study of its 5' flanking region. Serial deletion analysis of the -1863/+27 genomic fragment revealed the region -185/+27 as a core promoter. Cis-acting elements at the -182/-177 (E box) and -108/-104 (CCAAT box) positions were identified as the main activators of the Prnd expression in GC1-spg cells, with NF-Y being the important contributor. The gel-shift experiments confirmed the binding of the transcription factors to -108/-104 NF-Y consensus motif. An intron1 was found to enhance the Prnd expression in bEND.3 endothelial cells, suggesting an involvement of the endothelial-specific enhancer.
Nagyová
Janka
J
Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 05, Slovakia.
Pastorek
Jaromír
J
Kopácek
Juraj
J
D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000166:cytology; D023081:CCAAT-Binding Factor / Q000378:metabolism; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013737:Testis / Q000166:cytology / Q000502:physiology
D023081:CCAAT-Binding Factor; D058851:GPI-Linked Proteins; D011328:Prions; C122036:Prnd protein, mouse