[Analysis of human papillomavirus 16 E6 oncogene mutation in Xinjiang Uygur women with cervical carcinoma].
OBJECTIVE
High-risk human papillomaviruses(HPVs),such as HPV16, and HPV18,are major causes of cervical cancer (CC), and HPV16 was found most frequently in CC patients. HPV16E6 is one of major oncogenes. In some region, specific E6 mutation is considered as dangerous factor causing CC. There is a very high incidence of CC in southern Xinjiang, where the Uygur are the majority. As we reported before, we found HPV16E6 mutation from this district. This study was designed to investigate distribution of the mutation in CC of Xinjiang Uygur women, and the relationship between the mutation and high incidence of CC in southern Xinjiang.


METHODS
The tissue DNA was extracted from 35 CC biopsies of Xinjiang Uygur Women. HPV16E6 gene was amplified by polymerase chain reaction (PCR) from the CC tissue DNA. The PCR fragments were sequenced and analyzed.


RESULTS
The result of PCR showed that the positive rate of HPV16E6 was 82.86%(29/35); 26 of these 29 PCR fragments were sequenced and analyzed, 15 of them maintained prototype (57.69%), 11 have L83V mutation (34.62%), and 2 have L83V/D63E mutation (7.69%).


CONCLUSIONS
There is mutation within the HPV16E6 gene in CC of Xinjiang Uygur women. Our research suggested that the distribution of HPV16 prototype and HPV16E6 mutation might be associated with high incidence of CC in southern Xinjiang.
Ma
Zheng-Hai
ZH
Key Laboratory of Molecular Biology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumuqi, Xinjiang, 830046, PR China.
Zhang
Fu-Chun
FC
Mei
Xin-Di
XD
Ma
Cai-Ling
CL
Liu
Kai-Jiang
KJ
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000235:genetics; D017354:Point Mutation; D012097:Repressor Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000821:virology
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins