[Study on high efficiency tissue culture of mature seeds of rice (JiaHe-ZaoZhan)].
In order to improve the embryogenic callus induction rate and the regeneration rate of JiaHe-ZaoZhan rice, the influence of different factors were investigated, media with different hormones were used. Induction medium was supplemented with 1.5 mg/L 2,4-D + 3 mg/L NAA + 0.1 mg/L KT + 1 mg/L phytic acid + 20 mg/L PAA. Embryogenic call were treated under the condition of 25 degrees C before transferring to regeneration medium, the regeneration medium contained 0.5 mg/L 6-BA + 3 mg/L NAA + 0.5 mg/L KT + 1 mg/L phytic acid. The experiment results indicated that the hormone treatments had certain effects on the callus induction. Under the optimal medium, culture condition and the hormone treatments, the embryogenic callus induction could reach over 95%, and dry treatment of embryogenic callus had been found to increase the frequency of plant regeneration, significantly the plant regeneration rate could reach over 80%. Transplanted into pots, the young plants grew well. Then a experimental system with stability and high regenerating efficiency has been established for the mature seeds of rice (JiaHe-ZaoZhan).
Zhou
Ke Fu
KF
College of Life Science, Xiamen University, Ximen 361005.
Chen
Qi Wei
QW
Liu
Guang Fa
GF
Zheng
Qi Chang
QC
Yang
Guang
G
Wang
Hou Cong
HC
Chen
Liang
L
D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D012275:Oryza / Q000166:cytology* / Q000187:drug effects; D010463:Peracetic Acid / Q000494:pharmacology; D010833:Phytic Acid / Q000494:pharmacology; D012639:Seeds / Q000166:cytology* / Q000187:drug effects; D046509:Tissue Culture Techniques
D001224:Aspartic Acid; D010833:Phytic Acid; C000179:N-acetylaspartate; D010463:Peracetic Acid