Differential display analysis of mRNAs in chronic myelogenous leukaemia.
Analysis of expressed mRNAs with differential display-polymerase chain reaction (DD-PCR) is a powerful tool for the characterization of genes involved in malignant pathways and might identify markers for different phases of chronic myelogenous leukaemia (CML). We examined the presence of BCR-ABL transcripts in 25 CML patients in either the chronic phase or blast crisis. We then analysed the expression of leukocytic RNA transcripts in CML phases. DD-PCR technique was used to examine CML cases with BCR-ABL in comparison with CML cases lacking detectable BCR-ABL transcripts. Our results support the use of differential display not only for characterization of the CML differentially expressed genes but also to locate patterns that can be implemented as valuable fingerprints for each phase of CML.
el-Awady
M K
MK
Department of Human Genetics, National Research Centre, Cairo, Egypt.
Abdalla
N M
NM
Salem
A M
AM
Samir
S
S
Nour
A M
AM
D000293:Adolescent; D000328:Adult; D017398:Alternative Splicing / Q000235:genetics; D001345:Autoradiography; D014408:Biomarkers, Tumor / Q000235:genetics; D001752:Blast Crisis / Q000235:genetics; D016022:Case-Control Studies; D020732:Cytogenetic Analysis; D004534:Egypt / Q000453:epidemiology; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015996:Survival Rate; D014178:Translocation, Genetic / Q000235:genetics
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl