title: Reliability of intraocular pressure by Tono-Pen XL over amniotic membrane patch in human.
abstract: OBJECTIVE
  To determine the reliability of Tono-Pen XL in measuring intraocular pressure (IOP) over the amniotic membrane patch in human eyes.
  
  
  METHODS
  Ten healthy volunteers (nine males and one female) participated in this study. After topical anesthesia, IOP was measured using the Tono-Pen XL before and after the application of a single-layer amniotic membrane patch in the right eye and double-layer amniotic membrane patch in the left eye.
  
  
  RESULTS
  We found no statistical difference between the IOP measured with and without a single layer amniotic membrane (P = 0.808). Nor did we find any statistical difference between the IOP measured with and without double-layer amniotic membrane patch (P = 0.813). The average thickness of the single- and the double-layer amniotic membrane patch measured by pachymetry was 131.9 +/- 31.4 microm (mean +/- SD) and was 246.1 +/- 69.3 microm (mean +/- SD), respectively.
  
  
  CONCLUSIONS
  This study showed that it is reliable to measure IOP by Tono-Pen XL over single or a double layer of amniotic membrane patch on healthy human eyes. Further studies are required to determine the accuracy of IOP measurement over amniotic membrane patch on diseased corneas.
authors.affiliation: Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yoshita
authors.forename: Akira
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Mami
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sugiyama
mesh_terms: D000328:Adult; D001684:Biological Dressings; D005260:Female; D006801:Humans; D007429:Intraocular Pressure; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000295:instrumentation*