title: A liquid chromatography-mass spectrometric method for the determination of oak moss allergens atranol and chloroatranol in perfumes.
abstract: This paper describes a validated liquid chromatographic-tandem mass spectrometric method for quantitative analysis of the potential oak moss allergens atranol and chloroatranol in perfumes and similar products. The method employs LC-MS-MS with electrospray ionization (ESI) in negative mode. The compounds are analysed by selective reaction monitoring (SRM) of 2 or 3 ions for each compound in order to obtain high selectivity and sensitivity. The method has been validated for the following parameters: linearity; repeatability; recovery; limit of detection; and limit of quantification. The limits of detection, 5.0 ng/mL and 2.4 ng/mL, respectively, for atranol and chloroatranol, achieved by this method allowed identification of these compounds at concentrations below those causing allergic skin reactions in oak-moss-sensitive patients. The recovery of chloratranol from spiked perfumes was 96+/-4%. Low recoveries (49+/-5%) were observed for atranol in spiked perfumes, indicating ion suppression caused by matrix components. The method has been applied to the analysis of 10 randomly selected perfumes and similar products.
authors.affiliation: National Environmental Research Institute, Frederiksborgvej 399, 4000 Roskilde, Denmark.
authors.forename: Rossana
authors.initials: R
authors.lastname: Bossi
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Rastogi
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bernard
authors.forename: Elena
authors.initials: E
authors.lastname: Gimenez-Arnau
authors.forename: Jeanne D
authors.initials: JD
authors.lastname: Johansen
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Lepoittevin
authors.forename: Torkil
authors.initials: T
authors.lastname: Menné
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification*; D044002:Bryophyta / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010476:Perfume / Q000737:chemistry*
chemical_list: D000485:Allergens; D001547:Benzaldehydes; D010476:Perfume; C487450:chloroatranol