[The long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase of bone mesenchymal stem cells].
OBJECTIVE
To evaluate the long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase(ALP) of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially.


METHODS
Under cell culture technique, ALP kits were used to evaluate ALP level of bone mesenchymal stem cells so as to reflect differentiation of the cells on the 2nd, 5th, 7th, and 10th day by adding rhBMP-2 and rhbFGF on different ways.


RESULTS
Statistical analysis showed that rhBMP-2 could enhance while rhbFGF could inhibit the ALP level of rabbit bone mesenchymal stem cells significantly in long term, and also in a dose-dependent manner. The effects of using rhBMP-2 alone was similar to that of using the two growth factors associatedly, and they were significantly higher than that of using rhbFGF alone and using them sequentially.


CONCLUSIONS
Different growth factor has different long-term effect on differentiation of bone mesenchymal stem cells by adding them on different ways.
Hou
Rui
R
Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University, Xi'an 710032, China. hourui@fmmu.edu.cn
Mao
Tian-Qiu
TQ
Tao
Kai
K
Chen
Shu-Jun
SJ
Chen
Fu-Lin
FL
Zhao
Li-Jun
LJ
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; C100666:KCB-1 protein, recombinant; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D016222:Fibroblast Growth Factor 2; D000469:Alkaline Phosphatase