title: [Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants].
abstract: The encoding sequence of gus gene from Escherichia coli was fused with maize Ubi-1 promoter and was introduced into maize genome via particle bombardment. Fertile transgenic maize plants were regenerated from bombarded type-I calluses which were derived from scutellar tissue of immature embryos based on PPT selection. Expression activity of gus gene under the control of Ubi-1 promoter was analysed using histochemical method, and the results showed that gus gene expressed in most tissues except anther. Ubi-GUS expression in pollen, egg cell and T1 immature embryos revealed that this promoter was active in early stages of plant development. Histochemically stained pollen grains of T0 plants showed a 1:1 segregation of the gus gene, which suggested that the foreign gene was inherited in Mendelian model in these plants. In addition, maize Ubi-1 promoter could reduce the copy number of foreign genes in transgenic maize plants, which might be useful in avoidance of gene silencing. T1 seeds have been harvested.
authors.affiliation: Provincial Key Laboratory of Biotechnology, Northwest University, Xian 710069.
authors.forename: Zi Qin
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Jing Fen
authors.initials: JF
authors.lastname: Jia
authors.forename: Jian Guo
authors.initials: JG
authors.lastname: Hao
authors.forename: Ying Juan
authors.initials: YJ
authors.lastname: Wang
authors.forename: Shu Zhen
authors.initials: SZ
authors.lastname: Feng
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D025822:Ubiquitin C / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D010940:Plant Proteins; C417884:Ubi-1 protein, Zea mays; D025822:Ubiquitin C