Degradation of phytochrome interacting factor 3 in phytochrome-mediated light signaling.
Plant photoreceptors regulate various developmental processes. Among the photoreceptors, phytochromes, red and far-red light receptors, regulate light responses through many signaling components, including phytochrome-interacting proteins. The functional relationships among phytochromes and their interacting proteins, however, have not been clearly established. Here, we sought to identify a functional relationship between phytochromes and phytochrome interacting factor 3 (PIF3). We demonstrate that PIF3 is polyubiquitinated rapidly and subsequently degraded in PHYA and PHYB-mediated light signaling. We also show that the degradation of PIF3 is mediated by the 26S proteasome. Our data indicate that light-stimulated phytochromes cause the degradation of their interacting protein, PIF3, by the 26S proteasome.
Park
Eunae
E
Department of Biological Sciences, KAIST, Daejeon, 305-701 Korea.
Kim
Jonghyun
J
Lee
Yeon
Y
Shin
Jieun
J
Oh
Eunkyoo
E
Chung
Won-Il
WI
Liu
Jang Ryul
JR
Choi
Giltsu
G
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D051792:Basic Helix-Loop-Helix Proteins; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000528:radiation effects; D005809:Genes, Regulator / Q000235:genetics / Q000528:radiation effects; D008027:Light; D010775:Photic Stimulation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D010834:Phytochrome / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism / Q000528:radiation effects; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects*; D025801:Ubiquitin / Q000378:metabolism
D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D010834:Phytochrome; D046988:Proteasome Endopeptidase Complex; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; C116162:PIF3 protein, Arabidopsis