Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides.
When present, stilbene synthase leads to the production of resveratrol compounds, which are major components of the phytoalexin response against fungal pathogens of the plant and are highly bioactive substances of pharmaceutical interest. White poplar (Populus alba L.) was transformed with a construct containing a cDNA insert encoding stilbene synthase from grapevine (Vitis vinifera L.), under the control of the cauliflower mosaic virus (CaMV) 35S promoter, and a chimeric kanamycin resistance gene. Southern blot hybridization analysis demonstrated the presence and integration of exogenous DNA sequences in the poplar genome. Expression of the stilbene synthase-encoding gene in different transgenic lines was confirmed by Western blot and Northern analyses. Compared to the controls, in the transgenic plants two new compounds were detected and were identified as the trans- and cis-isomers of resveratrol-3-glucoside (piceid) by high-pressure liquid chromatography (HPLC), UV spectrophotometry, electrospray mass spectrometry (HPLC-ESI-MS) and enzymatic hydrolysis. Since poplar is a good biomass producer and piceids are accumulated in substantial amounts (up to 615.2 microg/g leaf fresh weight), the transgenic plants represent a potential alternative source for the production of these compounds with high pharmacological value. Despite the presence of piceid, in our experimental conditions no increased resistance against the pathogen Melampsora pulcherrima, which causes rust disease, was observed when in vitro bioassays were performed.
Giorcelli
Achille
A
Istituto di Sperimentazione per la Pioppicoltura-MiPAF, via di Frassineto 35, 1-15033 Casale Monferrato(AL), Italy.
Sparvoli
Francesca
F
Mattivi
Fulvio
F
Tava
Aldo
A
Balestrazzi
Alma
A
Vrhovsek
Urska
U
Calligari
Paolo
P
Bollini
Roberto
R
Confalonieri
Massimo
M
D000217:Acyltransferases / Q000378:metabolism*; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005960:Glucosides / Q000378:metabolism*; D006868:Hydrolysis; D013058:Mass Spectrometry; D030821:Plants, Genetically Modified; D032107:Populus / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013267:Stilbenes / Q000378:metabolism*; D014170:Transformation, Genetic; D027843:Vitis / Q000201:enzymology*
D018076:DNA, Complementary; D005960:Glucosides; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; C058229:polydatin