title: [Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV].
abstract: OBJECTIVE
  To investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV.
  
  
  METHODS
  HIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits.
  
  
  RESULTS
  All 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different.
  
  
  CONCLUSIONS
  The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.
authors.affiliation: Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Ai-jing
authors.initials: AJ
authors.lastname: Song
authors.forename: Xiu-hua
authors.initials: XH
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Si-hong
authors.initials: SH
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yan-min
authors.initials: YM
authors.lastname: Wan
authors.forename: Chun-tao
authors.initials: CT
authors.lastname: Zhang
authors.forename: You-chun
authors.initials: YC
authors.lastname: Wang
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006678:HIV / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood*; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic