Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial.
A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50% of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and beta2-glycoprotein I (beta2-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, beta2-GPI was also detected on all tested CVCs.
Bjerketorp
Joakim
J
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
Rosander
Anna
A
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
Nilsson
Martin
M
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
Jacobsson
Karin
K
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
Frykberg
Lars
L
Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D001672:Biocompatible Materials; D002352:Carrier Proteins / Q000378:metabolism; D002405:Catheterization, Central Venous; D005340:Fibrinogen / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology; D053482:beta 2-Glycoprotein I
D001426:Bacterial Proteins; D001672:Biocompatible Materials; D002352:Carrier Proteins; D006023:Glycoproteins; D019151:Peptide Library; C112405:Sbi protein, Staphylococcus aureus; D053482:beta 2-Glycoprotein I; D005340:Fibrinogen