[Bone formation by seeding bone marrow stromal cells on the sodium calcium phosphate/beta tricalcium phosphate scaffold].
OBJECTIVE
To observe the osteogenesis of sodium calcium phosphate/beta-tricalcium phosphate scaffold loaded with marrow stromal cells in a nude mice subcutaneous model.


METHODS
To transform the sintered bovine cancellous bone into NaCaPO(4)/beta-TCP biphasic calcium phosphate ceramic by physical and chemical methods. Bone marrow stromal cells (BMSCs) were obtained by harvesting the cancellous bone from the iliac crest of a rabbit. Then the BMSCs were isolated, expanded and induced in vitro and seeded in the NaCaPO(4)/beta-TCP ceramic scaffolds. The scaffold/BMSCs composites were implanted subcutaneously into the backs of nude mice. Implantation of NaCaPO(4)/beta-TCP ceramic alone was acted as control. At 4 and 8 weeks after implantation, the specimens were harvested and the osteogenetic activity were evaluated by gross and histologic observation.


RESULTS
At 4 weeks after implantation, the relatively mature bone were seen on the surface of the material, the inner part of the material were mainly cartilaginous bone. At 8 weeks after implantation, A large amount of trabecula bone had formed and the medullary cavity, medullary cells and lipocytes could be seen. Osteoblasts and osteoclasts were seen between the scaffolds and the local bone in the transition area. The endochondral bone formation pattern were observed.


CONCLUSIONS
The NaCaPO(4)/beta-TCP scaffold/BMSCs composites showed good osteogenetic activity and could promote mineralization of the immature bone. It can be used as the bone tissue engineering scaffolds.
Yang
Yao-Wu
YW
Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University, Xi'an 710032, Shanxi Province, China. yangyaowu@fm-mu.edu.cn
Lei
De-Lin
DL
Mao
Tian-Qiu
TQ
Hou
Rui
R
Li
Jian-Hu
JH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002130:Calcium Phosphates / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000166:cytology*; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods
D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C020243:calcium phosphate