title: Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
abstract: BACKGROUND
  Mast cells have long been recognized as the principal cell type that initiates the inflammatory response characteristic of acute allergic type 1 reactions. Our goal has been to further characterize maturation of progenitors to mast cells.
  
  
  METHODS
  Mast cells were cultured from human cord blood derived CD133(+) progenitors. Mast cell function was tested using histamine release. During differentiation mast cells surface marker expression was monitored by flow cytometry.
  
  
  RESULTS
  CD133(+) progenitors expressed the early haematopoietic and myeloid lineage markers CD34, CD117, CD13 and CD33. Mature mast cells expressed CD117, CD13 and CD33, and expression of the high affinity immunoglobulin E receptor FcepsilonRI increased during culture. Cytokine receptors interleukin (IL)-5R, IL-3R, granulocyte-macrophage-colony stimulating factor (GM-CSF)R and IL-18R were expressed at high levels during maturation. Chemokine receptors CXCR4 and CXCR2 were highly expressed on both newly purified CD133(+) cells and mature cells.
  
  
  CONCLUSIONS
  Human mast cells can be cultured from a CD34(+)/CD117(+)/CD13(+)/CD33(+) progenitor cell population in cord blood that is tryptase and chymase negative. Developing and mature mast cells express a wide range of chemokine and cytokine receptors. We found high levels of expression of CD123, IL-5R and GM-CSF receptors, also found on eosinophils and basophils, and high levels of expression of the receptor for the inflammatory cytokine IL-18.
authors.affiliation: Department of Paediatrics, Aarhus University Hospital, Aarhus N, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Dahl
authors.forename: H J
authors.initials: HJ
authors.lastname: Hoffmann
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: P O
authors.initials: PO
authors.lastname: Schiøtz
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000276:immunology; D002478:Cells, Cultured; D005312:Fetal Blood; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008407:Mast Cells / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000276:immunology; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000276:immunology; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000276:immunology; D016185:Receptors, Interleukin-3 / Q000096:biosynthesis* / Q000276:immunology; D053649:Receptors, Interleukin-5; D013234:Stem Cells / Q000276:immunology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D018121:Receptors, Cytokine; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5