Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800.
Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vivo synthesis of minicellulosomes suggests that it will be possible in the future to insert into B. subtilis cellulosomal genes that will allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.
Cho
Hee-Yeon
HY
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
Yukawa
Hideaki
H
Inui
Masayuki
M
Doi
Roy H
RH
Wong
Sui-Lam
SL
D001407:Bacillus / Q000302:isolation & purification*; D001483:Base Sequence; D003013:Clostridium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017931:DNA Primers; D015388:Organelles / Q000648:ultrastructure*; D010957:Plasmids; D015183:Restriction Mapping
D017931:DNA Primers