Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid.
DszB is a hydrolase involved in the biodegradation of dibenzothiophene in the soil bacterium Rhodococcus sp. IGTS8. DszB catalyzes the hydrolysis of 2'-hydroxybiphenyl-2-sulfinic acid to sulfite and biphenyl-2-ol. DszB and DszB C27S mutant complexed with biphenyl-2-sulfinic acid were crystallized and preliminary X-ray crystallographic analyses were conducted. The crystals of DszB were found to belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 36.7, b = 82.6, c = 139.6 A, and to contain one molecule of DszB in the asymmetric unit. Crystals of DszB C27S complexed with biphenyl-2-sulfinic acid belong to space group C2, with unit-cell parameters a = 153.4, b = 45.9, c = 112.9 A, beta = 115.93 degrees. The calculated Matthews coefficient V(M) for the C2 crystals is approximately 2.3 A(3) Da(-1) if two molecules of DszB are present in the asymmetric unit.
Lee
Woo Cheol
WC
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Ohshiro
Takashi
T
Matsubara
Toshiyuki
T
Izumi
Yoshikazu
Y
Tanokura
Masaru
M
D001713:Biphenyl Compounds / Q000737:chemistry*; D003460:Crystallization; D004926:Escherichia coli / Q000737:chemistry; D004950:Esterases / Q000737:chemistry*; D007202:Indicators and Reagents; D009154:Mutation / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology; D013441:Sulfinic Acids / Q000737:chemistry*; D014961:X-Ray Diffraction
D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D013441:Sulfinic Acids; C496673:biphenyl-2-sulfinic acid; D004950:Esterases