title: Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.
abstract: Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Cheng-qi
authors.initials: CQ
authors.lastname: Yan
authors.forename: Kai-xian
authors.initials: KX
authors.lastname: Qian
authors.forename: Gang-ping
authors.initials: GP
authors.lastname: Xue
authors.forename: Zhong-chang
authors.initials: ZC
authors.lastname: Wu
authors.forename: Yue-lei
authors.initials: YL
authors.lastname: Chen
authors.forename: Qiu-sheng
authors.initials: QS
authors.lastname: Yan
authors.forename: Xue-qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
mesh_terms: D024861:Genetic Enhancement; D006822:Hybrid Cells / Q000382:microbiology* / Q000502:physiology*; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics*; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics; D013045:Species Specificity