title: Successful pregnancy after preimplantation genetic diagnosis in a female with Robertsonian translocation.
abstract: Preimplantation genetic diagnosis (PGD) is an alternative option for couples with chromosome abnormalities. A 34-year-old woman with balanced Robertsonian translocation [(45, XX, der(13; 14)(q10; q10)] requested PGD due to recurrent spontaneous abortion. Embryos of good quality were biopsied on day 3 post-oocyte retrieval. The aspirated blastomeres were fixed and analyzed using fluorescence in situ hybridization. In the first cycle, 2 unaffected embryos were transferred back without success. No unaffected embryo was available in the second cycle. On day 5 in the third cycle, 2 unaffected embryos were transferred resulting in a twin pregnancy. Amniocentesis confirmed the diagnosis. At the gestational age of 35 weeks, 2 healthy girls were born via cesarean section. Postnatal physical examination found no evidence of major abnormalities.
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan.
authors.forename: Yueh-Chao
authors.initials: YC
authors.lastname: Wu
authors.forename: Chun-Kai
authors.initials: CK
authors.lastname: Chen
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Huang
authors.forename: Chi-Ju
authors.initials: CJ
authors.lastname: Chou
authors.forename: Yung-Kuei
authors.initials: YK
authors.lastname: Soong
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics; D000022:Abortion, Spontaneous / Q000235:genetics; D000328:Adult; D001755:Blastocyst / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic / Q000235:genetics*