title: Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea.
abstract: We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pig in Korea. In comparison with 22 fully sequenced JEV genomes currently available, we found that the 10,963-nucleotide RNA genome of KV1899 has a 13-nucleotide deletion in the 3' non-translated variable region and 53 unique nucleotide sequences including 3' non-translated region (NTR). Its single open reading frame has a total of 28 amino acid substitutions. Comparison of the KV1899 genomic sequence with those of the 21 fully sequenced JEV strains in published databases showed nucleotide homology ranging from 97.4% (Ishikawa strain) to 87.0% (CH2195 strain). Amino acid homology with KV1899 strain ranged from 96.4% (K94P05) to 91.0% (GP78). The KV1899 showed the highest nucleotide homology with Ishikawa strain and the highest amino acid homology with K94P05. We performed an extensive E gene based phylogenetic analysis on a selection of 41 JEV isolates available from the GenBank. Compared with Anyang strain, isolated from a pig in 1969, that is current live vaccine strain for swine in Korea, the homology of nucleotide sequence in envelope gene was only 87.1%. The prM gene of the isolate was closely related with those of Ishikawa and K94P05 strains, which were grouped into genotype I of JEV.
authors.affiliation: National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang 430-824, Korea. yangdk@nvrqs.go.kr
authors.forename: Dong Kun
authors.initials: DK
authors.lastname: Yang
authors.forename: Byoung Han
authors.initials: BH
authors.lastname: Kim
authors.forename: Chang Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: Jun Hun
authors.initials: JH
authors.lastname: Kwon
authors.forename: Seong In
authors.initials: SI
authors.lastname: Lim
authors.forename: Hong Ryul
authors.initials: HR
authors.lastname: Han
mesh_terms: D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D009033:Culicidae / Q000821:virology; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D007723:Korea; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus