title: Schizosaccharomyces pombe rsm1 genetically interacts with spmex67, which is involved in mRNA export.
abstract: We have previously isolated three synthetic lethal mutants from Schizosaccharomyces pombe in order to identify mutations in the genes that are functionally linked to spmex67 with respect to mRNA export. A novel rsm1 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMexl. The rsm1 gene contains no introns and encodes a 296 amino-acid-long protein with the RING finger domain, a C3HC4 in the N-terminal half. The deltarsm1 null mutant is viable, but it showed a slight poly(A)+ RNA accumulation in the nucleus. Also, the combination of deltarsm1 and deltaspmex67 mutations confers synthetic lethality that is accompanied by the severe poly(A)+ RNA export defect. These results suggest that rsm1 is involved in mRNA export from the nucleus.
authors.affiliation: Division of Biology, College of Natural Sciences, Kangnung National University, Gangneung 210-702, Korea. jhoyoon@kangnung.ac.kr
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D017353:Gene Deletion; D020043:Genes, Essential; D005800:Genes, Fungal; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D007438:Introns / Q000235:genetics; D009154:Mutation; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029543:Nucleocytoplasmic Transport Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins