[Determination of catalpol in dried rehmannia root and Taohong Siwu Decoction with high performance liquid chromatography].
OBJECTIVE
To determinate the catalpol contents in dried rehmannia root and Taohong Siwu Decoction containing rehmannia root with high performance liquid chromatography (HPLC).


METHODS
Catalpol was separated on a YWG-C18 column using water-acetonitrile (99.4:0.6) as mobile phase and detective wavelength at 210 nm.


RESULTS
The linear curve of tested catalpol concentration within the range of 0.0536-5.3600 microg/microl was ideal (n=5, r=0.999 7). The average recovery rate of the dried rehmannia root and Taohong Siwu decoction was 98.7% (RSD=0.48%) and 98.2% (RSD=1.29%) respectively.


CONCLUSIONS
HPLC method is accurate and valuable for the quality control of Radix Rehmanniae and Taohong Siwu Decoction.
Guo
Chun-Yan
CY
Department of Chemistry, Hebei North University, Zhangjiakou, Hebei Province 075000, China. chy-guo@sohu.com
Bai
Xue-Mei
XM
Zhang
Wan-Ming
WM
Wang
Zhi-Bao
ZB
Fu
Yu-Rong
YR
D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D032267:Rehmannia / Q000737:chemistry*
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; C078040:catalpol