[Isolation and culture of human pluripotent embryonic germ cells].
To establish human pluripotent embryonic germ (EG) cell lines, human primordial germ cells (PGCs) of embryos aborted in 5-9 week were cultured on inactive mouse STO fibroblast feeder. The medium contained human leukemia inhibitory factor (hLIF), human basic fibroblast growth factor (hbFGF) and forskolin. The EG cells could be passaged continuously until 12 generations. Most cells were positive in alkaline phosphatase staining and expressed cell surface antigen SSEA-3 and pluripotent marker Oct-4. These EG cell populations that retained normal karyotype could form embryoid body in culture and differentiate further into neuron-like cells, mucous epithelial cells, epithelial cells and other types of the cells spontaneously. These results indicated the cell clones derived from human PGCs resemble pluripotent EG cells from mouse PGCs in appearance or nature.
Li
Xiao Hong
XH
Laboratory of Tissue Engineering, Plastic Surgery, The 9th People's Hospital, Shanghai 2nd Medical University, Shanghai 200011.
Cong
Hsiao Chien
HC
Wang
Zheng
Z
Wu
Chun Fang
CF
Cao
Yi Lin
YL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism
D015295:Antigens, Tumor-Associated, Carbohydrate; D050814:Octamer Transcription Factor-3; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; D000469:Alkaline Phosphatase