Subcellular localization of WD40 repeat 1 protein in PC12 rat pheochromocytoma cells.
The dynamics of actin filament protein is crucial for various physiological processes of the cells. Among the proteins correlating with actin dynamics, a novel 67-kDa WD40 repeat protein 1 (WDR1) was the vertebrate homologue of actin-interacting protein 1 (Aip1). Even though previous studies have provided the clues on the function of WDR1 in specific organs under pathological conditions, the exact subcellular localization of WDR1 is not known. Therefore, in the present study, we undertook to determine the distribution of WDR1 within PC12 pheochromocytoma cells (PC12 cells) using light and electron microscopic techniques. Double immunocytochemistry clearly showed that WDR1 immunoreactivities (IRs) were co-localized with anti-actin antibody, suggesting the involvement of WDR1 in actin dynamics. WDR1 immunoreactivities (IRs) in PC12 cells showed different distribution patterns as nerve growth factor (NGF) concentrations varied. During active proliferation, the distribution of WDR1 IRs seemed to be similar to those found in cortical actin patches, whereas WDR1 IR was observed in cytoplasmic actin cables after PC12 cells were induced to differentiate by treating with NGF. Though further studies are necessary to determine the function of WDR1, the current data represents a first step towards the in vitro study of WDR1 protein.
Shin
Dong Hoon
DH
Cell Biology Laboratory for Neuroscience, Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong, Chongno-Gu, 110-799, South Korea. drdoogi@chollian.net
Lee
Eunju
E
Chung
Yoon Hee
YH
Mun
Ga Hee
GH
Park
Ji yeong
Jy
Lomax
Margaret I
MI
Oh
Seung Ha
SH
D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008840:Microfilament Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000379:methods; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000199:Actins; D008840:Microfilament Proteins; C494020:WD40 repeat 1 protein, rat; D020932:Nerve Growth Factor