title: Contribution of cell outer membrane and inner membrane to Cu2+ adsorption by cell envelope of Pseudomonas putida 5-x.
abstract: The role of outer and inner membrane in the Cu2+ adsorption process by gram-negative bacterium Pseudomonas putida 5-x, which was isolated from local electroplating effluent with high Cu2+ accumulating capability, was studied. The results indicate that both the outer and inner membrane exhibited high Cu2+ adsorption capacity. Outer and inner membrane contributed about 30-35% and 20-25% parts of adsorption capacity in Cu2+ adsorption by cell envelope of Pseudomonas putida 5-x, respectively. The total contribution of outer and inner membrane to Cu2+ adsorption by cell envelope was much greater than that of peptidoglycan layer. The relatively high phospholipid content in the outer membrane might result in its greater heavy metal ions adsorption capacity. The Cu2+ binding process by the outer and inner membrane of Pseudomonas putida 5-x is the adsorption processes and can be described with Freundlich isotherms.
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai, China. celwang@yahoo.com
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Chua
mesh_terms: D000327:Adsorption; D002462:Cell Membrane / Q000737:chemistry; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification*; D016958:Pseudomonas putida / Q000737:chemistry*; D013696:Temperature; D014873:Water Pollutants / Q000302:isolation & purification*
chemical_list: D014873:Water Pollutants; D003300:Copper