Propofol scavenges reactive oxygen species and inhibits the protein nitration induced by activated polymorphonuclear neutrophils.
Activated polymorphonuclear neutrophils may damage tissues through the release of biochemical mediators. Among them, peroxynitrite is responsible for hydroxylation reactions and nitration of proteins, or is metabolised into nitrate. We investigated the effect of propofol on the production of reactive oxygen species, the nitration of proteins and the formation of nitrate by activated human polymorphonuclear neutrophils. Propofol dose-dependently inhibited chemiluminescence, nitration of proteins and nitrate production in a concentration range from 10(-3) to 10(-6) mM. A significant correlation was observed between the logarithm of propofol concentration and the intensity of chemiluminescence (r2=0.90), the nitration of proteins (r2=0.67) and the production of nitrate (r2=0.79). Those results are consistent with the scavenging effect of propofol on peroxynitrite and could confer a protective property to propofol in pathological situations involving polymorphonuclear neutrophils activation.
Thiry
Jean-Christophe
JC
University Department of Anaesthesia and Intensive Care Medicine, CHR de la Citadelle, University Hospital, Boulevard du 12e de Ligne 1, 4000 Liege, Belgium.
Hans
Pol
P
Deby-Dupont
Ginette
G
Mouythis-Mickalad
Ange
A
Bonhomme
Vincent
V
Lamy
Maurice
M
D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D015742:Propofol / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D016166:Free Radical Scavengers; D009566:Nitrates; D009861:Onium Compounds; D011506:Proteins; D017382:Reactive Oxygen Species; C007517:diphenyleneiodonium; D013755:Tetradecanoylphorbol Acetate; D015742:Propofol