title: GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells.
abstract: The interleukin 7 receptor alpha-chain (IL-7Ralpha) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Ralpha expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Ralpha expression in T cells, where PU.1 is not expressed. Moreover, IL-7Ralpha expression was diminished substantially in thymocytes but was normal on B220(+) fetal liver cells from mouse embryos with diminished expression of GABPalpha. Thus, GABP is essential for the regulation of IL-7Ralpha expression in T cells, and the differential regulation of IL-7Ralpha in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N252, Bethesda, Maryland 20892-1674, USA.
authors.forename: Hai-Hui
authors.initials: HH
authors.lastname: Xue
authors.forename: Julie
authors.initials: J
authors.lastname: Bollenbacher
authors.forename: Valentina
authors.initials: V
authors.lastname: Rovella
authors.forename: Radhika
authors.initials: R
authors.lastname: Tripuraneni
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Du
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Liu
authors.forename: Ann
authors.initials: A
authors.lastname: Williams
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
authors.forename: Warren J
authors.initials: WJ
authors.lastname: Leonard
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000502:physiology*; D003850:Deoxyribonuclease I / Q000494:pharmacology; D051268:GA-Binding Protein Transcription Factor; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020395:Receptors, Interleukin-7 / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D051268:GA-Binding Protein Transcription Factor; C495656:Gabpa protein, mouse; D020395:Receptors, Interleukin-7; D014157:Transcription Factors; D003850:Deoxyribonuclease I