[The inflammatory mediators induced endothelial cell injury through delay neutrophils apoptosis].
This study aimed at elucidating the relationship between NTP apoptosis and endothelial cell injury. After isolation and purification, activated NTPs were incubated with endothelial cells directly or indirectly contact in a ratio of 5:1 and 10:1, meanwhile 10ng/ml IL-6 and 10% (v/v) burned serum added or not. Apoptosis and necrosis of endothelial cells were detected in situ. Activated NTP alone exerted no obviously detrimental effects on endothelial cells. However, when IL-6-or serum from burn patients was added, NTP apoptosis was delayed and endothelial cells were injured in the form of necrosis. Inflammatory mediators can delay NTP apoptosis, further research demonstrated that direct contact between the two types of cells was essential for NTP to impact injury on endothelial cells.
Liu
Ren
R
Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China. liu0916@mail.china.com
Xiao
Nan
N
Tian
Kun Lun
KL
Diao
You Fang
YF
D017209:Apoptosis / Q000187:drug effects*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects*
D018836:Inflammation Mediators; D015850:Interleukin-6