CD59a is the primary regulator of membrane attack complex assembly in the mouse.
Gene-deleted mice have provided a potent tool in efforts to understand the roles of complement and complement-regulating proteins in vivo. In particular, mice deficient in the membrane regulators complement receptor 1-related gene/protein y, decay-accelerating factor, or CD59 have demonstrated homeostatic relevance and backcrossing between the strains has revealed cooperativity in regulation. In mouse, genes encoding decay-accelerating factor and CD59 have been duplicated and show differential expression in tissues, complicating interpretation and extrapolation of findings to man. The first described form of CD59, CD59a, is broadly distributed and deletion of the cd59a gene causes a mild hemolytic phenotype with increased susceptibility in complement-mediated disease models. The distribution of the second form, CD59b, was originally described as testis specific, but later by some as widespread. Deletion of the cd59b gene caused a severe hemolytic and thrombotic phenotype. To apply data from these mouse models to man it is essential to know the relative distribution and functional roles of these two forms of CD59. We have generated new specific reagents and used them in sensitive quantitative analyses to comprehensively characterize expression of mRNA and protein and functional roles of CD59a and CD59b in wild-type (wt) and CD59a-negative mice. cd59b mRNA was detected only in testis and, at very low levels, in bone marrow. CD59b protein was present on mature spermatozoa and precursors and, in trace amounts, erythrocytes. Erythrocyte CD59b did not inhibit complement lysis except when CD59a was absent or blocked. These data confirm that CD59a is the primary regulator of complement membrane attack in mouse.
Baalasubramanian
Sivasankar
S
Complement Biology Group, Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Cardiff, United Kingdom.
Harris
Claire L
CL
Donev
Rossen M
RM
Mizuno
Masashi
M
Omidvar
Nader
N
Song
Wen-Chao
WC
Morgan
B Paul
BP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D018957:CD59 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D003167:Complement Activation / Q000276:immunology; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006461:Hemolysis / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009928:Organ Specificity / Q000276:immunology; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013737:Testis / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D018957:CD59 Antigens; D015938:Complement Membrane Attack Complex; D020033:Protein Isoforms; D012333:RNA, Messenger