[CD21-independent infection of a human signet ring cell gastric carcinoma cell line by Epstein-Barr virus].
OBJECTIVE
To understand Epstein-Barr virus (EBV) infection of gastric carcinoma cells.


METHODS
The authors tested the infection of a signet ring cell line HSC-39 derived from human gastric carcinoma with Akata and P3HR-1 strains of EBV. Akata and P3HR-1 infected of EBV cell clones were isolated by a limiting dilution method.


RESULTS
EBV-encoded small RNAs (EBERs) were expressed in the infected cells with each EBV strain by in situ hybridization. The EBV infected parental cells and most clones expressed EBNA1, but not EBNA2, latent membrane protein (LMP) 1 and LMP2A. Both EBV strains infected parental cells and clones presented type I latency. The uninfected HSC-39 cells were negative for CD21 expression; however, the Akata but not P3HR-1-infected clones were positive for CD21 expression at mRNA level.


CONCLUSIONS
These results demonstrated that EBV infecting HSC-39 by CD21-independent pathway. This study also defined a signet ring cell line as a new target for EBV.
Luo
Bing
B
Department of Microbiology, Qingdao University Medical College, Qingdao 266021, China. qdluobing@yahoo.com.
Masanao
Murakami
M
Makoto
Fukuta
F
Kazuoshi
Yanagihara
Y
Takeshi
Sairenji
S
D018279:Carcinoma, Signet Ring Cell / Q000473:pathology / Q000821:virology*; D045744:Cell Line, Tumor; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012333:RNA, Messenger; D012367:RNA, Viral / Q000032:analysis; D017464:Receptors, Complement 3d / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D013274:Stomach Neoplasms / Q000473:pathology / Q000821:virology*
D019309:Epstein-Barr Virus Nuclear Antigens; C099537:Epstein-Barr virus encoded RNA 1; D012333:RNA, Messenger; D012367:RNA, Viral; D017464:Receptors, Complement 3d; C417029:EBV-encoded nuclear antigen 1