title: DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis.
abstract: Broad-spectrum analysis of DNA and RNA samples is of increasing importance in the growing field of biotechnology. We show that nanopore measurements may be used to assess the purity, phosphorylation state, and chemical integrity of nucleic acid preparations. In contrast with gel electrophoresis and mass spectrometry, an unprecedented dynamic range of DNA sizes and concentrations can be evaluated in a single data acquisition process that spans minutes. Because the molecule information is quantized and digitally recorded with single-molecule resolution, the sensitivity of the system can be adjusted in real time to detect trace amounts of a particular DNA species.
authors.affiliation: Department of Molecular and Cellular Biology, Biological Laboratories, 16 Divinity Avenue, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: James E
authors.initials: JE
authors.lastname: Dunning
authors.forename: Albert P-H
authors.initials: AP
authors.lastname: Huang
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Nyamwanda
authors.forename: Daniel
authors.initials: D
authors.lastname: Branton
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004586:Electrophoresis / Q000379:methods*; D010766:Phosphorylation; D013696:Temperature
chemical_list: D004247:DNA