title: An attempt to render oocytes and embryos free from the porcine circovirus type 2 after experimental in vitro exposure.
abstract: The purpose of this study was to investigate the possibility of rendering oocytes and embryos free of porcine circovirus type 2 (PCV2). Groups of cumulus oocytes complexes, cumulus free oocytes, and embryos 3 to 5 d post breeding were exposed to PCV2 (10(5) TCID50/mL) prior to disinfection by washing and different combinations of enzymatic treatments. The study suggests that under the in vitro conditions used, standard washing procedures with, or without, trypsin or incorporating pronase or hyaluronidase and DNase/RNase in the treatment was not effective in rendering oocytes and embryos free from PCV2 nucleic acid. Since the virus is noncytopathic in cell culture and for embryonic cells, it appears that there is a possibility of introducing viral contamination through oocytes collected from infected pigs into the in vitro fertilization system with subsequent potential of producing in vitro fertilized embryos associated with PCV2.
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory-Fallowfield, Animal Diseases Research Institute, Germplasm Centre of Expertise, Nepean, Ontario. bielanskia@inspection.gc.ca
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bielanski
authors.forename: Renée
authors.initials: R
authors.lastname: Larochelle
authors.forename: Ronald
authors.initials: R
authors.lastname: Magar
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004622:Embryo, Mammalian / Q000821:virology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D009865:Oocytes / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D007507:Therapeutic Irrigation / Q000662:veterinary
chemical_list: D017931:DNA Primers; D004279:DNA, Viral