title: [Influence of nuclear factor-kappaB decoy transfection on nuclear factor-kappaB activity and vascular endothelial growth factor/urokinase-type plasminogen activator/intercellular cell adhesion molecule-1 level of SKOV3 cells].
abstract: OBJECTIVE
  To investigate the effect of nuclear factor-kappaB (NF-kappaB) decoy oligodeoxynucleotides (ODN) on NF-kappaB activation and expressions of vascular endothelial growth factor (VEGF), urokinase-type plasminogen activator (uPA), intercellular cell adhesion molecule-1 (ICAM-1) of SKOV3 cell.
  
  
  METHODS
  After transfected with NF-kappaB decoy ODN, SKOV3 cells were stimulated by IL-1beta and cultured for 6, 12, 24, and 48 h, respectively. NF-kappaB DNA binding activity was measured by gel mobility shift assay and mRNA levels of ICAM-1, uPA, VEGF were analyzed by RT-PCR and VEGF level in supernatant was detected by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  (1) SKOV3 cells expressed constitutive NF-kappaB DNA binding activity and stimulation of IL-1beta resulted in a significant increase of the binding activity. NF-kappaB decoy ODN transfection significantly decreased constitutive level and elevated level of NF-kappaB DNA binding activity by IL-1beta at 6, 12, 24, and 48 h in vitro (P < 0.05). (2) The constitutive and activated mRNA levels of uPA, VEGF, ICAM-1 and VEGF levels in the supernatant were significantly inhibited by NF-kappaB decoy ODN transfection especially at 6 h after transfection in vitro.
  
  
  CONCLUSIONS
  Both NF-kappaB DNA binding activity and expressions of uPA, VEGF, and ICAM-1 at 48 h were upregulated by IL-1beta, and inhibited by transfection with NF-kappaB decoy ODN in SKOV3 cell line. NF-kappaB decoy ODN transfection shows promise as a novel molecular approach for ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Chang-chun
authors.initials: CC
authors.lastname: Cao
authors.forename: Zhou-luo
authors.initials: ZL
authors.lastname: Ou
mesh_terms: D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000473:pathology; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D042461:Vascular Endothelial Growth Factor A; D018799:Intercellular Adhesion Molecule-1; D014568:Urokinase-Type Plasminogen Activator