title: Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae.
abstract: The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external glucose concentrations, irrespective of the presence of oxygen. In contrast, at low external glucose concentrations and in the presence of oxygen, as in a glucose-limited chemostat, no ethanol is produced. The importance of the external glucose concentration suggests a central role for the affinity and maximal transport rates of yeast's glucose transporters in the control of ethanol production. Here we present a series of strains producing functional chimeras between the hexose transporters Hxt1 and Hxt7, each of which has distinct glucose transport characteristics. The strains display a range of decreasing glycolytic rates resulting in a proportional decrease in ethanol production. Using these strains, we show for the first time that at high glucose levels, the glucose uptake capacity of wild-type S. cerevisiae does not control glycolytic flux during exponential batch growth. In contrast, our chimeric Hxt transporters control the rate of glycolysis to a high degree. Strains whose glucose uptake is mediated by these chimeric transporters will undoubtedly provide a powerful tool with which to examine in detail the mechanism underlying the switch between fermentation and respiration in S. cerevisiae and will provide new tools for the control of industrial fermentations.
authors.affiliation: Department of Chemistry and Bioscience-Molecular Biotechnology, Chalmers University of Technology, Box 462, SE-405 30 Göteborg, Sweden. Karin.Otterstedt@molbiotech.chalmers.se
authors.forename: Karin
authors.initials: K
authors.lastname: Elbing
authors.forename: Christer
authors.initials: C
authors.lastname: Larsson
authors.forename: Roslyn M
authors.initials: RM
authors.lastname: Bill
authors.forename: Eva
authors.initials: E
authors.lastname: Albers
authors.forename: Jacky L
authors.initials: JL
authors.lastname: Snoep
authors.forename: Eckhard
authors.initials: E
authors.lastname: Boles
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohmann
authors.forename: Lena
authors.initials: L
authors.lastname: Gustafsson
mesh_terms: D001692:Biological Transport; D000431:Ethanol / Q000378:metabolism; D005838:Genotype; D006019:Glycolysis; D006601:Hexoses / Q000378:metabolism*; D006706:Homeostasis; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D006601:Hexoses; D000431:Ethanol