Interleukin-6 produces neuronal loss in developing cerebellar granule neuron cultures.
CNS levels of the cytokine interleukin-6 (IL-6) are elevated during CNS injury and disease, but it is unclear if IL-6 contributes to the pathologic process. Our studies show that in a well-characterized CNS developmental model system, primary cultures of rodent cerebellar granule neurons, chronic exposure to IL-6 during neuronal development can result in cell damage and death in a subpopulation of developing granule neurons. Chronic exposure to IL-6 also increased the susceptibility of the granule neurons to a toxic insult produced by excessive activation of NMDA receptors. These results are consistent with a role for IL-6 in the neuropathology observed in the developing CNS during injury and disease.
Conroy
Shannon M
SM
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Nguyen
Vi
V
Quina
Lely A
LA
Blakely-Gonzales
Penney
P
Ur
Christina
C
Netzeband
Jeffrey G
JG
Prieto
Anne L
AL
Gruol
Donna L
DL
D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000166:cytology / Q000276:immunology*; D004347:Drug Interactions / Q000502:physiology; D015850:Interleukin-6 / Q000276:immunology / Q000633:toxicity*; D016202:N-Methylaspartate / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000276:immunology; D009498:Neurotoxins / Q000276:immunology / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects / Q000276:immunology
D015850:Interleukin-6; D009498:Neurotoxins; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate