title: Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier.
abstract: A porous calcium phosphate ceramic, which induced bone formation in soft tissues of dogs, was termed as osteoinductive biomaterial and studied as a carrier of bone morphogenetic protein (rhBMP-2). Cylinder implants (laser spot 4 x 5 mm) impregnated with 0, 1, 10 and 40 microg rhBMP-2 were implanted in dorsal muscles of rabbits for five weeks. Histological observation and histomorphometric analysis were performed on thin un-decalcified sections. No bone formation was detected in the implants without rhBMP-2, while mature lamellar bone was found inside the implants with 1 microg rhBMP-2, both on the outer surface and inside the implants with 10 microg and 40 microg rhBMP-2. Little difference in formed bone was found between 1 microg and 10 microg rhBMP-2, but no difference was found between 10 microg and 40 microg rhBMP-2. A significant difference in bone marrow formation was found among 1, 10 and 40 microg rhBMP-2. The more rhBMP-2, the more bone marrow formed. The present results indicate that osteoinductive biomaterial is a good carrier of BMP and high dose of BMP is not necessary for bone formation in clinic.
authors.affiliation: Biomaterials Research Group, Leiden University, The Netherlands, IsoTis B.V., Prof. Bronkhorstlaan 10, 3723 MB, Bilthoven, The Netherlands. huipin.yuan@isotis.com
authors.forename: H
authors.initials: H
authors.lastname: Yuan
authors.forename: J D
authors.initials: JD
authors.lastname: De Bruijn
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: C A
authors.initials: CA
authors.lastname: Van Blitterswijk
authors.forename: K
authors.initials: K
authors.lastname: De Groot