Gene silencing using a heat-inducible RNAi system in Arabidopsis.
Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.
Masclaux
Frédéric
F
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier, Pôle de Biotechnologie végétale, 24 chemin de Borde-Rouge, BP17, 31326 Castanet-Tolosan, France.
Charpenteau
Martine
M
Takahashi
Taku
T
Pont-Lezica
Rafael
R
Galaud
Jean-Philippe
JP
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D007438:Introns / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics*; D038761:Photobleaching; D011401:Promoter Regions, Genetic / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012987:Soil
D029681:Arabidopsis Proteins; C446287:HSP18.2 protein, Arabidopsis; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D012987:Soil; D010088:Oxidoreductases; C022398:phytoene dehydrogenase