A derivative of bovine herpesvirus 1 (BoHV-1) UL3.5 lacking the last forty amino acids inhibits replication of BoHV-1.
Bovine herpesvirus 1 (BoHV-1) UL3.5 is a tegument protein that interacts with BoHV-1 alpha-transinducing factor alphaBTIF). In this report, we show that a truncated UL3.5 lacking the last 40 amino acids (C40UL3.5) inhibited replication of BoHV-1. Stable expression of C40UL3.5 in MDBK cells inhibited replication of BoHV-1 300- to 500-fold in plaque assays. This inhibitory effect was specific for BoHV-1 as cells expressing C40UL3.5 supported replication of pseudorabies virus (species Suid herpesvirus 1, SuHV-1) and herpes simplex virus (species Human herpesvirus 1, HHV-1) to normal levels. In contrast, a truncated UL3.5 which lacked the first 20 amino acids and did not interact with alphaBTIF did not inhibit BoHV-1 replication. In the C40UL3.5-expressing cells infected with BoHV-1, expression of the viral immediate early gene BICP4 and the early protein gB were reduced and delayed. C40UL3.5, when either transiently or stably expressed, inhibited alphaBTIF-mediated transactivation of a BoHV-1 immediate-early promoter. C40UL3.5 may be useful for constructing transgenic cattle resistant to infection by BoHV-1.
Lam
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Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Letchworth
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D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D015534:Trans-Activators / Q000502:physiology; D014759:Viral Envelope Proteins / Q000096:biosynthesis; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000502:physiology*; D014764:Viral Proteins; D014779:Virus Replication / Q000502:physiology*
D015534:Trans-Activators; C108723:UL3.5 protein, virus; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C083786:immediate early protein BICP4, bovine herpesvirus 1