title: Hematopoietic activity of common marmoset CD34 cells isolated by a novel monoclonal antibody MA24.
abstract: OBJECTIVE
  We focused on a small New World monkey, the common marmoset (Callithrix jacchus), to establish a nonhuman primate model of the treatment of hematological disorders. In this study, we developed the first monoclonal antibodies (MAbs) against marmoset CD34 and tested the in vitro and in vivo hemopoietic activity of cell populations isolated using one of these MAbs.
  
  
  RESULTS
  Marmoset cDNA encoding a human CD34 homologue was cloned from bone marrow (BM)-derived RNA using reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. The amino acid sequence of the marmoset CD34 had 81% homology with the human sequence. Five mouse MAbs were raised against marmoset CD34 transfectant. One representative MAb, MA24 (IgM), reacted with approximately 0.5 to 1% of BM mononuclear cells (MNCs), where the colony-forming unit granulocyte/macrophage (CFU-GM) was enriched approximately 11- to 75-fold as compared with the whole BM MNCs. Multilineage differentiation of marmoset CD34+ cells in NOD/SCID mice was confirmed by flow cytometry 1 month after xenotransplantation.
  
  
  CONCLUSIONS
  These results demonstrated that MA24 is useful for the analysis and enrichment of hematopoietic progenitor cells in the marmoset model for preclinical experiments.
authors.affiliation: Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Izawa
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
authors.forename: Yukoh
authors.initials: Y
authors.lastname: Nakazaki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hibino
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Akemi
authors.initials: A
authors.lastname: Kawasaki
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Chieko
authors.initials: C
authors.lastname: Nishioka
authors.forename: Hajime
authors.initials: H
authors.lastname: Ishii
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Soda
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Arinobu
authors.initials: A
authors.lastname: Tojo
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Asano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D018952:Antigens, CD34 / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D002144:Callithrix / Q000097:blood*; D002469:Cell Separation / Q000379:methods*; D003001:Cloning, Molecular; D006410:Hematopoiesis; D008252:Macaca fascicularis; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D018952:Antigens, CD34