Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection.
An original system which uses Porous Graphitic Carbon as support and a mixture of organic solvents as mobile phase is proposed for the analysis of triterpenic acids by liquid chromatography. The separation of betulinic acid, ursolic acid, oleanolic acid, and 18alpha- and 18beta-glycyrrhetinic acids was carried out within a short time and monitored by evaporative light scattering detection as universal detection method. Molecular modelling studies show that the main contribution to the selectivity comes from the electrostatic interaction characterised by the dipole moment of the products.
Bérangère
Claude
C
Institut de Chimie Organique et Analytique, UMR CNRS 6005, Université d'Orléans, BP 6759, F-45067 Orléans Cedex 2, France.
Caussarieu
Nathalie
N
Morin
Philippe
P
Morin-Allory
Luc
L
Lafosse
Michel
M
D002244:Carbon / Q000737:chemistry*; D002853:Chromatography, Liquid; D006034:Glycyrrhetinic Acid / Q000032:analysis; D008027:Light; D008956:Models, Chemical; D008958:Models, Molecular; D009828:Oleanolic Acid / Q000032:analysis; D053978:Pentacyclic Triterpenes; D012542:Scattering, Radiation; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis; D014466:Ultraviolet Rays; D000094062:Betulinic Acid; D000097245:Ursolic Acid
D002244:Carbon; D006034:Glycyrrhetinic Acid; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid