title: 'Signature sets', minimal fragment sets for identifying protein disulfide structures with cyanylation-based mass mapping methodology.
abstract: Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods can overcome the failure to detect fragments by demonstrating the existence of small 'signature sets' of fragments. The link between signature sets and the robustness of CN-based methodology is validated by two case studies.
authors.affiliation: Departments of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Qi
authors.forename: Yi-Te
authors.initials: YT
authors.lastname: Chou
authors.forename: Eric
authors.initials: E
authors.lastname: Torng
authors.forename: J Throck
authors.initials: JT
authors.lastname: Watson
mesh_terms: D003486:Cyanides / Q000737:chemistry*; D003553:Cystine / Q000032:analysis* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: D003486:Cyanides; D004220:Disulfides; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D003553:Cystine