Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database (The Eimeria SCARdb).
This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all markers, revealing the presence of micro- and minisatellite repetitive units in 22 SCARs, with up to five distinct repeat units being observed per marker. Only 15 markers showed significant hits in similarity searches against public sequence databases, thus confirming their anonymous and non-coding character. Finally, a relational database of the markers (the Eimeria SCARdb) was developed and made available on the Internet, providing a valuable resource of SCAR markers that can be useful for molecular diagnosis, and also for epizootiological, genetic variability and genome mapping studies.
Fernandez
Sandra
S
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, USP, Av. Prof. Orlando Marques de Paiva 87, São Paulo, SP 05508-000, Brazil.
Katsuyama
Angela M
AM
Kashiwabara
André Y
AY
Madeira
Alda Maria B N
AM
Durham
Alan M
AM
Gruber
Arthur
A
D000818:Animals; D015139:Blotting, Southern; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D019295:Computational Biology; D016054:DNA, Protozoan / Q000737:chemistry* / Q000302:isolation & purification; D030561:Databases, Nucleic Acid; D004539:Eimeria / Q000235:genetics* / Q000302:isolation & purification*; D005819:Genetic Markers; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D011200:Poultry / Q000382:microbiology*; D011201:Poultry Diseases / Q000469:parasitology; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D005819:Genetic Markers