title: Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A-deficient mice.
abstract: Junctional adhesion molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. In the present work, we found that DCs also express JAM-A. To evaluate the biological relevance of this observation, Jam-A(-/-) mice were generated and the functional behavior of DCs in vitro and in vivo was studied. In vitro, Jam-A(-/-) DCs showed a selective increase in random motility and in the capacity to transmigrate across lymphatic endothelial cells. In vivo, Jam-A(-/-) mice showed enhanced DC migration to lymph nodes, which was not observed in mice with endothelium-restricted deficiency of the protein. Furthermore, increased DC migration to lymph nodes was associated with enhanced contact hypersensitivity (CHS). Adoptive transfer experiments showed that JAM-A-deficient DCs elicited increased CHS in Jam-A(+/+) mice, further supporting the concept of a DC-specific effect. Thus, we identified here a novel, non-redundant role of JAM-A in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
authors.affiliation: Department of Vascular Biology, Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology, Milan, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Cera
authors.forename: Annalisa
authors.initials: A
authors.lastname: Del Prete
authors.forename: Annunciata
authors.initials: A
authors.lastname: Vecchi
authors.forename: Monica
authors.initials: M
authors.lastname: Corada
authors.forename: Ines
authors.initials: I
authors.lastname: Martin-Padura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Motoike
authors.forename: Paolo
authors.initials: P
authors.lastname: Tonetti
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Bazzoni
authors.forename: William
authors.initials: W
authors.lastname: Vermi
authors.forename: Francesca
authors.initials: F
authors.lastname: Gentili
authors.forename: Sergio
authors.initials: S
authors.lastname: Bernasconi
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Sato
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Dejana
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000172:deficiency* / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology*; D042783:Endothelial Cells; D005455:Fluorescent Antibody Technique; D062726:Junctional Adhesion Molecules; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D015815:Cell Adhesion Molecules; D062726:Junctional Adhesion Molecules