title: The influence of dispersant concentration on the pore morphology of hydroxyapatite ceramics for bone tissue engineering.
abstract: There is a clinical need for synthetic scaffolds that will promote bone regeneration. Important factors include obtaining an optimal porosity and size of interconnecting windows whilst maintaining scaffold mechanical strength, enabling complete penetration of cells and nutrients throughout the scaffold, preventing the formation of necrotic tissue in the centre of the scaffold. To address this we investigated varying slip deflocculation in order to control the resulting porosity, pore size and interconnecting window size whilst maintaining mechanical strength. Hydroxyapatite (HA) porous ceramics were prepared using a modified slip casting process. Rheological measurements of the HA slips were used to identify deflocculation conditions which resulted in changes in the cell and window sizes of the resulting ceramics. Sintered ceramics were characterised by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Pore and window size distribution was determined by SEM. XRD analysis confirmed that the crystal structure remained HA after the sintering process. SEM showed that HA porous ceramics presented a highly interconnected porous network with average pore sizes ranging from 391+/-39 to 495+/-25 microm. The average window size varied from 73+/-5 to 135+/-7 microm. Pore diameters obtained were controllable in the range 200-500 microm. Window sizes were in the range 30-250 microm. The use of dispersant concentration allows pore and window size to be modified whilst maintaining control over porosity demonstrated by a porosity of 85% for seven different dispersant concentrations. The advantage of this approach allows the correlation between the rheological conditions of the slip and the resultant sintered ceramic properties. In particular, optimising the ceramic strength by controlling the agglomeration during the casting process.
authors.affiliation: School of Mechanical, Materials, Manufacturing Engineering and Management, University of Nottingham, University Park, Nottingham NG7 2RD, UK. lesley.cyster@nottingham.ac.uk
authors.forename: L A
authors.initials: LA
authors.lastname: Cyster
authors.forename: D M
authors.initials: DM
authors.lastname: Grant
authors.forename: S M
authors.initials: SM
authors.lastname: Howdle
authors.forename: F R A J
authors.initials: FR
authors.lastname: Rose
authors.forename: D J
authors.initials: DJ
authors.lastname: Irvine
authors.forename: D
authors.initials: D
authors.lastname: Freeman
authors.forename: C A
authors.initials: CA
authors.lastname: Scotchford
authors.forename: K M
authors.initials: KM
authors.lastname: Shakesheff
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008968:Molecular Conformation; D010316:Particle Size; D016062:Porosity; D011208:Powders; D013501:Surface-Active Agents / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D011208:Powders; D013501:Surface-Active Agents; D017886:Durapatite