title: Molecular cloning and characterization of SGT1.2, a novel splice variant of Homo sapiens SGT1.
abstract: SGT1.2, a novel splice variant of Homo sapiens SGT1 was isolated from a human fetal brain cDNA library. This cDNA is 1404 bp and contains an open reading frame from 68 to 1165 encoding a putative protein of 365 amino acids (SGT1.2) that share 90% identities to Homo sapiens SGT1, suppressor of G2 allele of SKP1 at protein level. RPS-BLAST searching revealed that SGT1.2 have a TPR domain, a p23 domain, a SGS domain and a CS domain. According to the search of the human genome database, SGT1.2 was mapped to human chromosome 13q14.13. Reversed transcription-polymerase chain reaction analysis indicated that it widely expressed in human adult tissues.
authors.affiliation: a State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Xianqiong
authors.initials: X
authors.lastname: Zou
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Maoqing
authors.initials: M
authors.lastname: Wu
authors.forename: Huarui
authors.initials: H
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D014644:Genetic Variation; D015186:Hominidae; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C490164:SUGT1 protein, human