[Effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes].
OBJECTIVE
To observe the effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes.


METHODS
3H-glucose uptaking rate in adipocytes treated by salidroside was detected. Adipocytes treated by salidroside in differentiation were stained by oil red O and analyzed by spectrophotography quantitatively. The expression of PPAR-gamma and C/EBP-alpha mRNA relating to differentiation of adipocytes was detected by reverse transcription PCR.


RESULTS
3H-glucose uptaking rate in the salidroside group was 110.4%, higher than that in the blank control group (P < 0.01). Salidroside suppressed the differentiation of 3T3-L1 pre-adipocytes and down-regulated the expression of PPAR-gamma and C/EBP-alpha mRNA (P < 0.01, vs control group).


CONCLUSIONS
Salidroside promotes the 3H-glucose uptaking, suppresses the differentiation and down-regulates the expression of PPAR-gamma and C/EBP-alpha mRNA in 3T3-L1 adipocytes.
Wang
Shu-Hai
SH
Institute of Integrated Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China. wshuhai@hotmail.com
Wang
Wen-Jian
WJ
Wang
Xue-Feng
XF
Chen
Wei-Hua
WH
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077154:Rosiglitazone; D013053:Spectrophotometry; D013251:Steroid Isomerases / Q000235:genetics / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology
D002352:Carrier Proteins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D010636:Phenols; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C490380:Ebp protein, mouse; D013251:Steroid Isomerases; D005947:Glucose; C009172:rhodioloside