PCR-based identification of hyperthermophilic archaea of the family Thermococcaceae.
A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5'-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3') and TcPc 589R (5'-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3') was developed and used for identification of new isolates.
Slobodkina
Galina B
GB
Institute of Microbiology, Russian Academy of Sciences, prospekt 60-letiya Oktyabrya 7/2, Moscow, 117312 Russia.
Chernyh
Nikolai A
NA
Slobodkin
Alexander I
AI
Subbotina
Irina V
IV
Bonch-Osmolovskaya
Elizaveta A
EA
Lebedinsky
Alexander V
AV
D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D005843:Geography; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D019710:Thermococcaceae / Q000235:genetics* / Q000302:isolation & purification*
D017931:DNA Primers