title: High-throughput production of optimized primers (fimers) for whole-genome direct sequencing.
abstract: Fimers are specifically modified primers selected to inhibit nonspecific interactions occurring in cycle sequencing. They are postsynthetically derived from 2'-methoxyoxalamido or 2'-succinimido precursor oligonucleotides by treatment with appropriate small molecular weight modifiers (a primary aliphatic amine or hydroxide anion). We describe design, synthesis, and purification of fimers, and their use in protocols for direct sequencing of genomic deoxyribonucleic acid (DNA). Protocol for isolation of microbial genomic DNA is also reported.
authors.affiliation: Fidelity Systems Inc., Gaithresburg, MD, USA
authors.forename: Nikolai
authors.initials: N
authors.lastname: Polushin
authors.forename: Andrei
authors.initials: A
authors.lastname: Malykh
authors.forename: A Michael
authors.initials: AM
authors.lastname: Morocho
authors.forename: Alexei
authors.initials: A
authors.lastname: Slesarev
authors.forename: Sergei
authors.initials: S
authors.lastname: Kozyavkin
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D016678:Genome
chemical_list: D017931:DNA Primers