[Effect of Yiqi Huayu Recipe on regeneration process after L5 nerve root compression in rats].
OBJECTIVE
To study the effect of Yiqi Huayu Recipe on neurons in neuromuscular junction after L5 nerve root compression in rats.


METHODS
By using immunohistochemistry, confocal laser scanning techniques and protein gene product 9.5 as a neuronal marker, the motor endplates were visualized with fluorescein-conjugated alpha-BTX. The effect of Yiqi Huayu Recipe on nerve terminals of neuromuscular junction at 10, 20, 30, 60 days after L5 nerve root compression was observed in rat soleus muscles during regeneration. The overlap areas of terminals and endplates were measured with NIH image technique.


RESULTS
As for the aggregates, sprouts, extension of nerve terminal during regeneration, the connecting speed and range of nerve terminal with endplates, as well as the reformation of neuromuscular junctions during regeneration, Yiqi Huayu Recipe group was better than that of the control group.


CONCLUSIONS
Yiqi Huayu Recipe can promote the hyperplasia of neurons and enhance the regeneration function. It also can accelerate the neuromuscular junction reformation and shorten the process of regeneration.
Zhou
Chong-Jian
CJ
Institute of Spinal Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Wang
Yong-Jun
YJ
Shi
Qi
Q
Hou
Bao-Xing
BX
Liu
Mei
M
D000818:Animals; D008297:Male; D008516:Medicine, Chinese Traditional; D009408:Nerve Compression Syndromes / Q000473:pathology / Q000503:physiopathology; D009416:Nerve Regeneration / Q000187:drug effects*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000032:analysis
C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase