[The regulation of PKCalpha in eosinophil infiltration and proliferation in nasal polyps].
OBJECTIVE
To explore the regulation of protein kinase C (PKC) isoform--PKCalpha in eosinophil (EOS) proliferation and infiltration in nasal polyp tissues.


METHODS
With the methods of in situ hybridization staining and immunohistochemistry MGG staining, to check out the relationship between PKC and bcl-2/BaxmRNA and associated protein, especially PKC isoform--PKCalpha, PKCbeta1 , PKCbeta2, and PKCgamma did not express at all.


RESULTS
There were PKC expression in the eosinophils of 26 cases from nasal polyps, and the expression of PKC and Bcl-2 mRNA/their protein in EOS of nasal polyps showed remarkably positive relation (r1 = 0.0875, r2 = 0.0823, P < 0.01), but in PKC isoforms, PKCalpha expression was the strongest, but PKCbeta1 and PKCbeta2 expressed thinner and PKCgamma did not express at all.


CONCLUSIONS
The reason of eosinophil proliferation and infiltration in nasal polyps is that PKC signal transduction pathway was activated, and leaded to inhibition of eosinophil apoptosis, and eosinophil survival was delayed, and eosinophil proliferated and infiltrated, and in PKC family, PKCalpha is main.
Li
Meihua
M
Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Third Clinical Hospital of Jilin University, Changchun, 130031, China.
Yang
Zhanquan
Z
Song
Wenzhi
W
D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D004804:Eosinophils / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000201:enzymology / Q000473:pathology*; D011493:Protein Kinase C / Q000502:physiology*; D051571:Protein Kinase C-alpha
C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha