Cell-surface alterations in class IIa bacteriocin-resistant Listeria monocytogenes strains.
Strains of the food-borne pathogen Listeria monocytogenes, showing either intermediate or high-level resistance to class IIa bacteriocins, were investigated to determine characteristics that correlated with their sensitivity levels. Two intermediate and one highly resistant spontaneous mutant of L. monocytogenes B73, a highly resistant mutant of L. monocytogenes 412, and a highly resistant, defined (mptA) mutant of L. monocytogenes EGDe were compared with their respective wild-type strains in order to investigate the contribution of different factors to resistance. Decreased mannose-specific phosphotransferase system gene expression (mptA, EIIAB(Man) component) was implicated in all levels of resistance, confirming previous studies by the authors' group. However, a clear correlation between d-alanine content in teichoic acid (TA), in particular the alanine : phosphorus ratio, and a more positive cell surface, as determined by cytochrome c binding, were found for the highly resistant strains. Furthermore, two of the three highly resistant strains showed a significant increase in sensitivity towards d-cycloserine (DCS). However, real-time PCR of the dltA (d-alanine esterification), and dal and ddlA genes (peptidoglycan biosynthesis) showed no change in transcriptional levels. The link between DCS sensitivity and increased d-alanine esterification of TA may be that DCS competes with alanine for transport via the alanine transporter. A possible tendency towards increased lysinylation of membrane phospholipid in the highly resistant strains was also found. A previous study reported that cell membranes of all the resistant strains, including the intermediate resistant strains, contained more unsaturated phosphatidylglycerol, which is an indication of a more fluid cell membrane. The results of that study correlate with the possible lysinylation, decreased mptA expression, d-alanine esterification of TA and more positive cell surface charge found in this study for resistant strains. The authors' findings strongly indicate that all these factors could contribute to class IIa bacteriocin resistance and that the combination and contribution of each of these factors determine the level of bacteriocin resistance.
Vadyvaloo
Viveka
V
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
Arous
Safia
S
Laboratoire de Microbiologie Fondamentale et Appliquée, CNRS FRE 2224, IBMIG, UFR Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Gravesen
Anne
A
Department of Dairy and Food Science, Centre for Advanced Food Studies, LMC, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
Héchard
Yann
Y
Laboratoire de Microbiologie Fondamentale et Appliquée, CNRS FRE 2224, IBMIG, UFR Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
Chauhan-Haubrock
Ramola
R
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
Hastings
John W
JW
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
Rautenbach
Marina
M
Department of Biochemistry, University of Stellenbosch, Private Bag X1, 7602 Matieland, South Africa.
D000409:Alanine / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry; D003523:Cycloserine / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000378:metabolism*; D008239:Lysine / Q000032:analysis; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis / Q000235:genetics; D010715:Phosphatidylglycerols / Q000032:analysis; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013682:Teichoic Acids / Q000737:chemistry
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010457:Peptidoglycan; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013682:Teichoic Acids; D045304:Cytochromes c; D003523:Cycloserine; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase; D008239:Lysine; D000409:Alanine