title: Active and low-active forms of serum phospholipid transfer protein in a normal Finnish population sample.
abstract: Human serum phospholipid transfer protein (PLTP) exists as a catalytically active (HA-PLTP) and a low-active (LA-PLTP) form. In this study, the association of PLTP activity and the concentrations of both forms with lipid and carbohydrate parameters were investigated. In a random Finnish population sample, serum PLTP concentration (n=250) was 6.56 +/- 1.45 mg/l, the mean lipoprotein-independent (PLTPexo) phospholipid transfer activity was 6.59 +/- 1.66 micromol/ml/h, and the mean lipoprotein-dependent (PLTPendo) activity was 1.37 +/- 0.29 micromol/ml/h. Of the serum PLTP concentration, approximately 46% was in a catalytically active form. HA-PLTP concentration correlated positively with serum PLTPexo activity (r=0.380, P <0.001), HDL cholesterol (r=0.291, P <0.001), and apolipoprotein A-I (r=0.187, P <0.01). Of the potential regulatory factors for PLTP, apolipoprotein E showed a weak positive correlation with serum PLTPexo (r=0.154, P <0.05) and PLTPendo (r=0.192, P <0.01) activity but not with PLTP concentration. Weak associations were also observed between PLTP parameters and determinants of glucose homeostasis (glucose, insulin, and homeostasis model assessment for insulin resistance). The present data on PLTP activity and concentration reveal novel connections of the two PLTP forms to lipid and carbohydrate metabolism.
authors.affiliation: Department of Molecular Medicine, National Public Health Institute, Biomedicum, Helsinki, Finland.
authors.forename: Minna T
authors.initials: MT
authors.lastname: Jänis
authors.forename: Sarah
authors.initials: S
authors.lastname: Siggins
authors.forename: Esa
authors.initials: E
authors.lastname: Tahvanainen
authors.forename: Riikka
authors.initials: R
authors.lastname: Vikstedt
authors.forename: Kaisa
authors.initials: K
authors.lastname: Silander
authors.forename: Jari
authors.initials: J
authors.lastname: Metso
authors.forename: Arpo
authors.initials: A
authors.lastname: Aromaa
authors.forename: Marja-Riitta
authors.initials: MR
authors.lastname: Taskinen
authors.forename: Vesa M
authors.initials: VM
authors.lastname: Olkkonen
authors.forename: Matti
authors.initials: M
authors.lastname: Jauhiainen
authors.forename: Christian
authors.initials: C
authors.lastname: Ehnholm
mesh_terms: D008076:Cholesterol, HDL / Q000378:metabolism; D005260:Female; D005387:Finland; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008875:Middle Aged; D049294:Phospholipid Transfer Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008076:Cholesterol, HDL; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins