Alternative modalities of adenovirus-mediated gene expression in hippocampal neurons cultured on microisland substrate.
Previously, we have used CsCl gradient-purified recombinant adenovirus (AdV) to successfully transfer genes into hippocampal neurons cultured on microisland substrate. Here, we report that purification of AdV particles is not required and efficient gene expression can be achieved using either crude AdV lysates or HEK 293 cells infected with AdV. The advantages of the simplified procedure are greatly reduced preparation time and reduced requirements for equipment and expertise.
Chen
Huanmian
H
Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Park Bldg. Room 150, 12420 Parklawn Drive, MSC 8115, Bethesda, MD 20892-8815, USA. huanchen@mail.nih.gov
Honse
Yumiko
Y
Ikeda
Stephen R
SR
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001418:Baclofen / Q000494:pharmacology; D002478:Cells, Cultured; D004347:Drug Interactions; D004622:Embryo, Mammalian; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000235:genetics; D018755:GABA Agonists / Q000494:pharmacology; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000494:pharmacology; D015870:Gene Expression / Q000502:physiology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006624:Hippocampus / Q000166:cytology* / Q000821:virology; D006801:Humans; D008564:Membrane Potentials / Q000235:genetics; D009474:Neurons / Q000378:metabolism* / Q000821:virology; D018408:Patch-Clamp Techniques; D037342:Pertussis Toxin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D018755:GABA Agonists; D037342:Pertussis Toxin; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D001418:Baclofen