title: Isomerase-independent chaperone function of cyclophilin ensures aggregation prevention of adenosine kinase both in vitro and under in vivo conditions.
abstract: Using inactive aggregates of adenosine kinase (AdK) from Leishmania donovani as the model substrate, we recently demonstrated that a cyclophilin (LdCyP) from the same source in an isomerase-independent fashion reactivated the enzyme in vitro by disaggregating its inactive oligomers [Chakraborty et al. (2002) J. Biol. Chem. 277, 47451-47460]. Besides disrupting preformed aggregates, LdCyP also prevents reaggregation of the newly formed active protein that is generated after productive refolding from its urea-denatured state. To investigate possible physiological implications of such phenomena, a unique expression system that simultaneously induces both AdK and LdCyP in naturally AdK-deficient Escherichia coli, was developed. Both in vitro and in vivo experiments revealed that oligomerization is an inherent property of this particular enzyme. In vivo protein cross-linking studies, activity determination analysis and Ado phosphorylation experiments carried out in cells coexpressing both the proteins unequivocally demonstrated that, similar to the phenomena observed in vitro, aggregates of the enzyme formed in vivo are able to interact with both LdCyP and its N-terminal truncated form (N(22-88)DEL LdCyP) in a crowded intracellular environment, resulting in aggregation prevention and reactivation of the enzyme. Our results indicate that the isomerase-independent chaperone function of LdCyP, detected in vitro, participates in vivo as well to keep aggregation-prone proteins in a monomeric state. Furthermore, analogous to yeast/bacterial two-hybrid systems, development of this simple coexpression system may help in the confirmation of interaction of two proteins under simulated in vivo conditions.
authors.affiliation: Division of Infectious Diseases, Leishmania Group, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata-700 032, India.
authors.forename: Anutosh
authors.initials: A
authors.lastname: Chakraborty
authors.forename: Banibrata
authors.initials: B
authors.lastname: Sen
authors.forename: Rupak
authors.initials: R
authors.lastname: Datta
authors.forename: Alok K
authors.initials: AK
authors.lastname: Datta
mesh_terms: D000248:Adenosine Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D021983:Cyclophilins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D007535:Isomerases / Q000235:genetics / Q000378:metabolism*; D007893:Leishmania donovani / Q000201:enzymology; D008954:Models, Biological; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D020673:Protein Renaturation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D021122:Protein Subunits; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D000248:Adenosine Kinase; D007535:Isomerases; D021983:Cyclophilins