title: Support for a proposed retinoid-processing protein complex in apical retinal pigment epithelium.
abstract: The interaction of cellular retinaldehyde-binding protein (CRALBP) with ERM (ezrin, radixin, moesin)-binding phosphoprotein 50 (EBP50) in retinal pigment epithelium (RPE) microsomes has led to the hypothesis that a retinoid-processing protein complex exists in apical RPE. Mouse RPE apical processes were isolated on wheat germ agglutinin-coated agarose beads. Proteomic analyses of the isolated apical RPE demonstrated the presence of CRALBP, EBP50, 11-cis-retinol dehydrogenase, cellular retinol-binding protein 1, and interphotoreceptor retinoid-binding protein. The results support the hypothesis that a visual cycle protein complex may serve in the localization and release of 11-cis-retinoid in the apical RPE.
authors.forename: Vera L
authors.initials: VL
authors.lastname: Bonilha
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: Karen A
authors.initials: KA
authors.lastname: West
authors.forename: John S
authors.initials: JS
authors.lastname: Crabb
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Mary E
authors.initials: ME
authors.lastname: Rayborn
authors.forename: Maria
authors.initials: M
authors.lastname: Nawrot
authors.forename: John C
authors.initials: JC
authors.lastname: Saari
authors.forename: John W
authors.initials: JW
authors.lastname: Crabb
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D005136:Eye Proteins / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D010750:Phosphoproteins; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D012172:Retinaldehyde / Q000378:metabolism; D012176:Retinoids / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000032:analysis; D054840:Retinol-Binding Proteins, Cellular; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D000429:Alcohol Oxidoreductases; D002352:Carrier Proteins; D005136:Eye Proteins; D010750:Phosphoproteins; D012172:Retinaldehyde; D012176:Retinoids; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D017923:Sodium-Hydrogen Exchangers; D000099214:Retinol-Binding Proteins, Interstitial; C020932:11-cis-retinal-binding protein; C093376:sodium-hydrogen exchanger regulatory factor; C022520:retinol dehydrogenase