Exon 6 of human Jagged-1 encodes an autonomously folding unit.
Human Jagged-1 is predicted to contain 16 epidermal growth factor-like (EGF) repeats. The oxidative folding of EGF-2, despite the several conditions tested, systematically led to complex mixtures. A longer peptide spanning the C-terminal part of EGF-1 and the complete EGF-2 repeat, on the contrary, could be readily refolded. This peptide, which corresponds to the entire exon 6 of the Jagged-1 gene, thus represents an autonomously folding unit. We show that it is structured in solution, as suggested by circular dichroism and NMR spectroscopy, and displays an EGF-like disulfide bond topology, as determined by disulfide mapping.
Guarnaccia
Corrado
C
International Centre for Genetic Engineering and Biotechnology (ICGEB), Protein Structure and Bioinformatics Group, AREA Science Park, Padriciano 99, I-34012 Trieste, Italy.
Pintar
Alessandro
A
Pongor
Sándor
S
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins; D002942:Circular Dichroism; D005091:Exons; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017510:Protein Folding; D017386:Sequence Homology, Amino Acid; D000072099:Serrate-Jagged Proteins; D013056:Spectrophotometry, Ultraviolet
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins