Both leptin and leptin-receptor are essential for apolipoprotein M expression in vivo.
It was previously shown that circulating levels of leptin and apolipoprotein M (apoM) correlate to each other. In this study, we examined whether plasma leptin and leptin-receptors are of importance for apoM expression in vivo. It was found that in both liver and kidney, expression of apoM was significantly lower in leptin deficient ob/ob mice and in leptin-receptor deficient db/db mice than in control mice. Furthermore, leptin administration (0.5 or 1.5 microg/g body weight) significantly increased plasma apoM levels and apoM mRNA levels in liver and in kidney in ob/ob mice. We conclude that both leptin and leptin-receptor are essential for the apoM expression, indicating that leptin is physiologically regulating apoM synthesis in vivo.
Xu
Ning
N
Department of Clinical Chemistry, Institute of Laboratory Medicine, University Hospital of Lund, S-221 85 Lund, Sweden. ning.xu@klinkem.lu.se
Nilsson-Ehle
Peter
P
Hurtig
Maria
M
Ahrén
Bo
B
D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000075243:Apolipoproteins M; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney / Q000378:metabolism; D020738:Leptin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D054834:Lipocalins; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008820:Mice, Obese; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D054411:Receptors, Leptin
C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D018076:DNA, Complementary; C515448:LEPR protein, human; D020738:Leptin; D054834:Lipocalins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse