Construction of a transplantable tissue-engineered artificial peritoneum.
BACKGROUND
Peritoneal defects lead to serious postoperative problems. Thus the development of physiological material to cover peritoneal defects is very desirable.


OBJECTIVE
The aim of this study was to develop a transplantable artificial peritoneum.


METHODS
The artificial peritoneum consisted of collagen gel, fibroblasts, and mesothelial cells, and histological features were analyzed. The artificial peritoneum at the site of a peritoneal defect in the rat was transplanted to the abdominal wall.


RESULTS
Histological examination revealed that the artificial peritoneum consisted of a flat mesothelial monolayer upon a stromal matrix. All transplanted artificial peritoneums adapted well to the host and prevented severe adhesion.


CONCLUSIONS
Our artificial peritoneum may be a useful transplantable bioengineered material for repair of surgical peritoneal defects.
Kuga
H
H
Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Morisaki
T
T
Nakamura
K
K
Onishi
H
H
Matsuda
T
T
Sueishi
K
K
Tanaka
M
M
Katano
M
M
D034861:Abdominal Wall / Q000601:surgery; D000818:Animals; D001187:Artificial Organs; D002448:Cell Adhesion; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010537:Peritoneum / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D000267:Tissue Adhesions / Q000517:prevention & control; D023822:Tissue Engineering