Molecular cloning and identification of a novel oxygenase gene specifically induced during the growth of Rhodococcus sp. strain T104 on limonene.
Rhodococcus sp. strain T104 is able to utilize both limonene and biphenyl as growth substrates. Furthermore, T104 possesses separate pathways for the degradation of limonene and biphenyl. Previously, we found that a gene(s) involved in limonene degradation was also related to indigo-producing ability. To further corroborate this observation, we have cloned and sequenced a 8,842-bp genomic DNA region with four open reading frames, including one for indole oxygenase, which converts indole to indigo (a blue pigment), The reverse transcription PCR data demonstrated that the identified indole oxygenase gene is specifically induced by limonene, thereby implicating this gene in the degradation of limonene by T104.
Choi
Ki Young
KY
Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Korea.
Kim
Dockyu
D
Koh
Sung-Cheol
SC
So
Jae-Seong
JS
Kim
Jong-Sul
JS
Kim
Eungbin
E
D003001:Cloning, Molecular; D053138:Cyclohexenes; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D000077222:Limonene; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010105:Oxygenases / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013729:Terpenes / Q000378:metabolism*
D053138:Cyclohexenes; D004269:DNA, Bacterial; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013729:Terpenes; D000077222:Limonene; D010105:Oxygenases; D049308:Dioxygenases