Chemiluminescence: comparison of whole blood with isolated polymorphonuclear leukocytes after major trauma.
BACKGROUND
Neutrophil (PMN) mediated tissue injury is central to the development of post-traumatic ARDS/MOF. Changes in activity caused by PMN isolation may be avoided by studying respiratory burst activity using whole blood chemiluminescence (WBCL).


METHODS
WBCL and PMNCL were measured in 5 normal laboratory volunteers (NLV) and 9 patients sustaining major trauma, within 2 hours of admission. Receptor mediated (STZ) and independent (PMA) activating agents were used.


RESULTS
PMA activation confirmed significant priming both in WBCL and PMNCL after major trauma. With STZ, priming was confirmed in the WBCL study, but the trauma patient PMNCL showed no difference in response to those of NLV. Although the study population was small, those patients later developing ARDS demonstrated significantly greater STZ activated WBCL, 8 hours after admission.


CONCLUSIONS
PMN isolation alters behavior in vitro. This may lead to important differences of in vivo PMN function being obscured when studied in the laboratory setting. Further study of CL response and surface receptor expression is clearly warranted, both in WB and PMN preparations.
Pallister
Ian
I
Department of Trauma & Orthopaedics, Morriston Hospital, Morriston, Swansea, England, United Kingdom. ianpallister@hotmail.com
Topley
Nicholas
N
D016022:Case-Control Studies; D002469:Cell Separation / Q000379:methods / Q000592:standards; D018450:Disease Progression; D006801:Humans; D007249:Inflammation; D015601:Injury Severity Score; D008163:Luminescent Measurements; D016177:Macrophage-1 Antigen / Q000276:immunology; D009102:Multiple Organ Failure / Q000209:etiology; D009104:Multiple Trauma / Q000097:blood* / Q000150:complications / Q000276:immunology*; D009249:NADP / Q000276:immunology; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D018579:Patient Selection; D011237:Predictive Value of Tests; D017382:Reactive Oxygen Species / Q000276:immunology; D016897:Respiratory Burst / Q000276:immunology; D012128:Respiratory Distress Syndrome / Q000209:etiology; D012307:Risk Factors; D018709:Statistics, Nonparametric; D013755:Tetradecanoylphorbol Acetate; D013997:Time Factors; D015054:Zymosan
D016177:Macrophage-1 Antigen; D017382:Reactive Oxygen Species; D009249:NADP; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate