Human mesenchymal progenitor cell-based tissue engineering of a single-unit osteochondral construct.
A desirable strategy for articular cartilage repair is to surgically replace the damaged area with an in vitro-engineered osteochondral plug. We report here the development of a novel osteochondral construct using human trabecular bone-derived mesenchymal progenitor cells and a biodegradable poly-D,L-lactic acid scaffold. The cartilage layer was fabricated by press-coating a chondrifying high-density cell pellet onto the scaffold, which was then loaded with cells previously initiated to undergo osteogenesis. The composite was then cultured in a cocktail medium formulated to maintain both chondrogenesis and osteogenesis. Macroscopically, the construct consisted of a cartilage-like layer adherent to, and overlying, a dense bone-like component. RT-PCR, immunohistochemistry, and histology revealed hyaline-like cartilage and bone with an interface resembling the native osteochondral junction. All parameters, including mechanical properties, improved with increased culture time. The single-cell source nature of the construct, which minimizes handling while maximizing biocompatibility, suggests applicability for articular cartilage repair.
Tuli
Richard
R
Cartilage Biology and Orthopedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-8022, USA.
Nandi
Sumon
S
Li
Wan-Ju
WJ
Tuli
Suraj
S
Huang
Xiaoxue
X
Manner
Paul A
PA
Laquerriere
Patrice
P
Nöth
Ulrich
U
Hall
David J
DJ
Tuan
Rocky S
RS
D020341:Absorbable Implants; D000328:Adult; D021522:Bioartificial Organs; D002358:Cartilage, Articular / Q000254:growth & development / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology; D019245:Compressive Strength; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)