title: Construction of a transplantable tissue-engineered artificial peritoneum.
abstract: BACKGROUND
  Peritoneal defects lead to serious postoperative problems. Thus the development of physiological material to cover peritoneal defects is very desirable.
  
  
  OBJECTIVE
  The aim of this study was to develop a transplantable artificial peritoneum.
  
  
  METHODS
  The artificial peritoneum consisted of collagen gel, fibroblasts, and mesothelial cells, and histological features were analyzed. The artificial peritoneum at the site of a peritoneal defect in the rat was transplanted to the abdominal wall.
  
  
  RESULTS
  Histological examination revealed that the artificial peritoneum consisted of a flat mesothelial monolayer upon a stromal matrix. All transplanted artificial peritoneums adapted well to the host and prevented severe adhesion.
  
  
  CONCLUSIONS
  Our artificial peritoneum may be a useful transplantable bioengineered material for repair of surgical peritoneal defects.
authors.affiliation: Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kuga
authors.forename: T
authors.initials: T
authors.lastname: Morisaki
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Onishi
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Sueishi
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Katano
mesh_terms: D034861:Abdominal Wall / Q000601:surgery; D000818:Animals; D001187:Artificial Organs; D002448:Cell Adhesion; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010537:Peritoneum / Q000601:surgery; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D000267:Tissue Adhesions / Q000517:prevention & control; D023822:Tissue Engineering