title: Large-scale methacrylate monolithic columns: design and properties.
abstract: Monoliths represent a special class of chromatographic supports. In contrast to other stationary phases, they consist of a single piece of highly porous material through which a sample is mainly transported by convection. As a consequence, monoliths enable fast separations and exhibit flow-unaffected properties, which make them attractive for purification of macromolecules like proteins or DNA. In this work, methacrylate-based monolithic columns with the bed volume up to 8000 ml are characterized. They perform high-resolution separations of several hundreds of grams of proteins per hour by utilizing liter per minute flow rates. They are incompressible under these operating conditions and resistant to strong alkaline conditions.
authors.affiliation: BIA Separations d.o.o., Teslova 30, SI-1000, Ljubljana, Slovenia. ales.podgornik@monoliths.com
authors.forename: A
authors.initials: A
authors.lastname: Podgornik
authors.forename: J
authors.initials: J
authors.lastname: Jancar
authors.forename: M
authors.initials: M
authors.lastname: Merhar
authors.forename: S
authors.initials: S
authors.lastname: Kozamernik
authors.forename: D
authors.initials: D
authors.lastname: Glover
authors.forename: K
authors.initials: K
authors.lastname: Cucek
authors.forename: M
authors.initials: M
authors.lastname: Barut
authors.forename: A
authors.initials: A
authors.lastname: Strancar
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D008689:Methacrylates; D010455:Peptides; D011506:Proteins