Improved resolution power of electrophoretic fractionation of DNA using a voltage gradient "up and down" application.
The improved resolution power of electrophoretic fractionation of DNA in a wide range of molecular masses is demonstrated using an "up and down" application of voltage gradient gel electrophoresis (VGGE). This application also allows separation of different DNA fragments which are poorly fractionated in conventional electrophoresis.
Bellavia
Daniele
D
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Palermo, Italy.
Sanfilippo
Rosalia
R
Sisino
Giorgia
G
Palmeri
Laura
L
Barbieri
Rainer
R
D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D004247:DNA