Effect of ionizing radiation on gene expression in CD4+ T lymphocytes and in Jurkat cells: unraveling novel pathways in radiation response.
To better understand at the molecular level the effect of ionizing radiation in leukocytes, the global transcriptional response to X-ray irradiation was studied in human CD4+ T lymphocytes and in Jurkat cells. Microarray analysis performed on freshly isolated human CD4+ lymphocytes 8 h after an LD50 irradiation dose of 1 Gy revealed that out of 13,825 genes, 1084 were modulated more than 1.5-fold. The most strongly up-regulated genes were predominantly p53 targets. In contrast, exposure of the CD4+ T lymphocyte-derived Jurkat leukemic cell line (with no functional p53 gene) to an equivalent LD50 dose (0.5 Gy) induced a partly different and more limited set of genes. Interestingly, this set of genes belonged to the Rho and cytokine signaling pathways regulated by low-dose ionizing radiation.
Mori
M
M
Laboratory of Radiobiology, Belgian Nuclear Research Centre (SCK.CEN), Boeretang, Mol, Belgium. mmori@sckcen.be
Benotmane
M A
MA
Vanhove
D
D
van Hummelen
P
P
Hooghe-Peters
E L
EL
Desaintes
C
C
D017209:Apoptosis / Q000528:radiation effects; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D003599:Cytoskeleton / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D019169:Jurkat Cells; D015398:Signal Transduction / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D016159:Tumor Suppressor Protein p53; D020741:rho GTP-Binding Proteins