title: Temporal profile of ultrastructural changes in cortical neurons after a photochemical lesion.
abstract: A photochemical lesion was induced in the right sensory motor cortex of rat brains. We examined at various time points the occurrence of different types of neuronal death with respect to a potential therapeutic window. The lesion appearance was documented by magnetic resonance imaging, and functional recovery was evaluated by behavioral tests showing recovery at 48 hr after lesioning. At 0.5, 1, 3, 6, 12, 24, 48, and 72 hr postlesion, cortical layers IV and V were examined by light and electron microscopy. Ultrastructural changes, which corresponded well to light microscopy findings, were found in both hemispheres. In the lesioned area, the neuropil appeared disorganized at 0.5 hr, and apoptotic and necrotic cell death was found at 0.5-3 hr. After 3 hr, the tissue was disintegrated. On the contralateral side, chromatin clumping appeared at 0.5-3 hr. At 3 hr, ruptured membranes were found, a sign of irreversible cell death. At 6-72 hr, the membranes were intact, and the chromatin was not clumped but heterogeneously distributed. The nuclei contained dispersed nucleoli at 48-72 hr. The morphology correlated well with magnetic resonance images and functional behavior. Our study demonstrates that a photochemical lesion is a useful model for studying morphological changes in injured cells. It results in a permanent infarction within 3 hr. In that the morphology on the contralateral side drastically changed between 3 and 6 hr, the cellular alterations at these time points might represent a break point at which cells either progress toward cell death or recover.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden. benita.andersson@cns.ki.se
authors.forename: Benita
authors.initials: B
authors.lastname: Andersson
authors.forename: Xingchen
authors.initials: X
authors.lastname: Wu
authors.forename: Börje
authors.initials: B
authors.lastname: Bjelke
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D002540:Cerebral Cortex / Q000473:pathology* / Q000648:ultrastructure*; D008297:Male; D009474:Neurons / Q000473:pathology* / Q000648:ultrastructure*; D010777:Photochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors