The ABC's (and XYZ's) of peptide sequencing.
Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key - but little understood - technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.
Steen
Hanno
H
Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
Mann
Matthias
M
D000595:Amino Acid Sequence; D019295:Computational Biology; D016208:Databases, Factual; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000295:instrumentation / Q000379:methods*
D010455:Peptides; D020033:Protein Isoforms