Protection of atlantic salmon Salmo salar against infectious pancreatic necrosis after DNA vaccination.
Although vaccines against infectious pancreatic necrosis (IPN) based on inactivated virus or recombinant structural viral proteins are commercially available, the protection is not complete and the disease is still a problem for the Atlantic salmon Salmo salar farming industry. In the present study, 5 different plasmids that expressed whole or parts of the large open reading frames (ORF) of Segment A of the IPN virus (IPNV) were constructed. The plasmids were shown to express proteins in cell cultures and in zebrafish Danio rerio in vivo. The specificities of the expressed proteins were confirmed by staining with IPNV-specific monoclonal antibodies (MAb) The plasmids were then used alone or in different combinations to vaccinate groups of Atlantic salmon, which subsequently were challenged in an experimental assay for IPN. A high level of protection was induced only by the plasmid combination that contained a plasmid expressing all the large ORF polyprotein.
Mikalsen
Aase B
AB
Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, PO Box 8146 Dep, 0033 Oslo, Norway.
Torgersen
Jacob
J
Aleström
Peter
P
Hellemann
Anne-Lill
AL
Koppang
Erling-Olaf
EO
Rimstad
Espen
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017756:Aquaculture / Q000379:methods; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D015153:Blotting, Western; D002478:Cells, Cultured; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology*; D018061:Infectious pancreatic necrosis virus / Q000235:genetics*; D009664:Norway; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D020317:Salmo salar; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish
D000911:Antibodies, Monoclonal; D019444:Vaccines, DNA; D015678:Viral Structural Proteins