The interaction of copper-bipyridyl complex with DNA and cleavage to DNA.
The interaction of a copper-bipyridyl (bpy) complex with CT-DNA was investigated by voltammetry, absorption and fluorescence spectrophotometry. The binding constant of the Cu(bpy)2(2+) complex interacting with DNA was 3.24 x 10(4) L/mol and the ability binding of Cu(bpy)2(2+) to DNA was 1.3-times as large as that of Cu(bpy)2+ to DNA. DNA could be efficiently cleaved by a potential-modulated method in the presence of the Cu(bpy)2(2+) complex. The fragments of the cleaved DNA were separated by high-performance liquid chromatography (HPLC). The experimental results revealed that the proposed method for DNA cleavage is highly efficient.
Yang
Zhou-Sheng
ZS
School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P R, China. yzhoushe@mail.ahnu.edu.cn
Wang
Yan-Ling
YL
Zhao
Guang-Chao
GC
D015082:2,2'-Dipyridyl / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D009942:Organometallic Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D009942:Organometallic Compounds; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA