title: Electrophoresis in organogels.
abstract: A new matrix for electrophoresis, a low molecular weight organogel, is described. Dansylated amino acids and peptides were separated by planar and capillary electrophoresis in acetonitrile gels of trans-(1S,2S)-1,2-bis(dodecylamido)cyclohexane. The superior separation ability of the organogel over its corresponding buffer solution in capillary electrophoresis is illustrated. Organogels provide all the advantages associated with planar matrixes with 100% efficient recovery and transfer of the analytes to a mass spectrometer. We demonstrate that the planar gel can be liquefied and injected as is into an ESI-MS to identify the separands.
authors.affiliation: Casali Institute of Applied Chemistry, Chemistry Institute, Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
authors.forename: Shaul
authors.initials: S
authors.lastname: Mizrahi
authors.forename: Jenny
authors.initials: J
authors.lastname: Gun
authors.forename: Z Gidon
authors.initials: ZG
authors.lastname: Kipervaser
authors.forename: Ovadia
authors.initials: O
authors.lastname: Lev