Effects of ovariohysterectomy on canine blood neutrophil respiratory burst: a chemiluminescence study.
OBJECTIVE
To examine blood neutrophil counts and luminol-enhanced chemiluminescence (CL) responses in dogs undergoing ovariohysterectomy (OH), premedicated with 2 different drugs.


METHODS
Randomized clinical study.


METHODS
Forty-two healthy client-owned bitches.


METHODS
Dogs had OH under isoflurane anesthesia with either acepromazine or medetomidine, both in combination with butorphanol, administered as preanesthetic medication. Blood samples were collected when the dog was admitted, at the end of surgery, and the next day (approximately 20 hours after surgery). Blood neutrophils were counted automatically, and neutrophil oxidative activity was assessed by measuring blood CL responses (induced by opsonized zymosan and enhanced by luminol) at 37 degrees C for 40 minutes.


RESULTS
Number of circulating neutrophils was significantly increased the day after surgery reflected by enhanced blood CL responses. Neutrophil CL, however, was not significantly altered. No significant differences were detected for perioperative Polymorphonuclear neutrophil (PMN) characteristics between the 2 preanesthetic regimens.


CONCLUSIONS
In conclusion, despite clearly increasing the number of circulating neutrophils, OH did not significantly affect neutrophil respiratory burst, as measured by whole-blood CL responses.


CONCLUSIONS
Surgical operation of moderate intensity (e.g., OH) did not significantly alter one of the important immune functions, neutrophil oxidative activity. Further studies are warranted to confirm the significance of this finding, and to assess the value of following this variable in different animal patient populations.
Väisänen
Misse
M
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. misse.vaisanen@helinski.fi
Lilius
Esa-Matti
EM
Mustonen
Leena
L
Raekallio
Marja
M
Kuusela
Erja
E
Koivisto
Maarit
M
Vainio
Outi
O
D000758:Anesthesia / Q000662:veterinary*; D000818:Animals; D004285:Dogs / Q000097:blood / Q000276:immunology / Q000502:physiology* / Q000601:surgery; D005260:Female; D007044:Hysterectomy / Q000662:veterinary*; D008163:Luminescent Measurements / Q000662:veterinary; D009504:Neutrophils / Q000502:physiology*; D010052:Ovariectomy / Q000662:veterinary*; D011184:Postoperative Period; D011229:Preanesthetic Medication / Q000662:veterinary*; D016897:Respiratory Burst