title: Biotin-labeled oligonucleotides with extraordinarily long tethering arms.
abstract: We describe synthesis of four novel biotin phosphoramidites with tethering arms ranging from 20 to 74 atoms in length. One of these phosphoramidites is a uridine derivative with a biotin moiety attached through the 2'-position. The biotin phosphoramidites were synthetized based on robust and efficient methoxyoxalamido (MOX) and succinimido (SUC) precursor strategies from MOX/SUC precursors containing a secondary hydroxyl. They are highly stable in solution (coupling efficiency remains equally high for at least 2 wk after phosphoramidite installation on the synthesizer) and are ideal for the streamlined production of biotin-labeled oligonucleotides. Protocols for the synthesis of biotinylated primers and purification of biotinylated sequencing products by means of streptavidin-coated magnetic beads are also presented.
authors.affiliation: Fidelity Systems Inc., Gaithersburg, MD, USA.
authors.forename: A Michael
authors.initials: AM
authors.lastname: Morocho
authors.forename: Valeri
authors.initials: V
authors.lastname: Karamyshev
authors.forename: Olga
authors.initials: O
authors.lastname: Shcherbinina
authors.forename: Nikolai
authors.initials: N
authors.lastname: Polushin
mesh_terms: D001710:Biotin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D001710:Biotin