title: State of the art of trichothecenes analysis.
abstract: Methods to analyze trichothecenes should be fast, reliable and economical. The known methods can be divided into two categories: the 'instrumental methods' as gas chromatography (GC) and high performance liquid chromatography (HPLC) and the so called 'fast methods' like thin layer chromatography (TLC), enzyme-linked immunosorbant analyses (ELISA) and flow through immunoassays. The most frequently used instrumental methods for the determination of deoxynivalenol (DON), HT-2- and T2-toxin in cereals and cereal products are based on gas chromatography with electron capture detectors or mass spectrometric (MS) detectors. More than 70% of the literature published in the last decade focuses on these techniques. Recently, high performance liquid chromatography with fluorescence detection and with mass spectrometric detection have gained importance. Up to now, a method which is able to detect types A and B trichothecenes simultaneously with a sufficiently low limit of detection is lacking, even though methods are published which have at least the same clean up step.
authors.affiliation: Swiss Quality Testing Services, Grünausteasse 23, CH-8953 Dietikon, Switzerland. priska.koch@sqts.ch
authors.forename: Priska
authors.initials: P
authors.lastname: Koch
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D014255:Trichothecenes; D013605:T-2 Toxin; C007262:deoxynivalenol; C012351:HT-2 toxin