Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development.
The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.
Naested
Henrik
H
Institute of Molecular Biology, Copenhagen University, Øster Farimagsgade 2A, 1353 Copenhagen K, Denmark.
Holm
Agnethe
A
Jenkins
Tom
T
Nielsen
H Bjørn
HB
Harris
Cassandra A
CA
Beale
Michael H
MH
Andersen
Mathias
M
Mant
Alexandra
A
Scheller
Henrik
H
Camara
Bilal
B
Mattsson
Ole
O
Mundy
John
J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D047908:Intracellular Signaling Peptides and Proteins; D008027:Light; D008969:Molecular Sequence Data; D010641:Phenotype; D010858:Pigmentation; D010860:Pigments, Biological / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010860:Pigments, Biological; D011993:Recombinant Fusion Proteins; C504622:VAR3 protein, Arabidopsis