cDNA cloning of translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) from the intertidal harpacticoid copepod Tigriopus japonicus.
We synthesized a cDNA library from the intertidal copepod Tigriopus japonicus, converted it to phagemids and sequenced expressed sequence tags (ESTs). Of these, Tigriopus translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) was further characterized. The Tigriopus TCTP/HRF gene encoded 172 amino acid residues and showed high similarity to Drosophila but moderate similarity to other annelids (e.g. Brugia, Wuchereria and C. elegans). The Tigriopus TCTP/HRF gene appeared in the same clade as the annelids. Here, we describe the analysis of the Tigriopus TCTP/HRF gene.
Kim
Il-Chan
IC
Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, South Korea.
Kim
Young Ja
YJ
Lee
Young-Mi
YM
Kim
Bok-Geon
BG
Park
Tae-Jin
TJ
Kim
Hyeung-Sin
HS
Jung
Min-Min
MM
Williams
Tim D
TD
Lee
Wonchoel
W
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D003001:Cloning, Molecular; D033342:Copepoda / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D000090324:Tumor Protein, Translationally-Controlled 1