[Construction of guided bone regeneration membrane by tissue engineering in vitro].
In this study, porous polymer (PLA/PCL) membrane was first treated with ethanol to become hydrophilic, and then immersed into DMEM with 50% fetal bovine serum to enhance the affinity to cells. MSCs cultured in osteogenic medium were loaded into the membrane at density of 5 x 10(6)/cm2 for 7 days, and scanning electrical microscope was used to observe the growth of the MSCs. The growth of MSCs inside the constructs was functionally well, and the cells proliferated with the time of culture. We concluded from current study that the membrane had satisfactory biocompatibility and the constructs could be used to guided bone regeneration.
Huang
Lanfeng
L
Department of Orthopaedics, The Second hospital, Jilin University, Changchun 130041, China.
Qi
Xin
X
Liu
Jianguo
J
Xu
Xinxiang
X
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D018786:Bone Substitutes; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005260:Female; D048091:Guided Tissue Regeneration; D019344:Lactic Acid; D008566:Membranes; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters; D011108:Polymers; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide)