Genetic basis of tobacco smoking: strong association of a specific major histocompatibility complex haplotype on chromosome 6 with smoking behavior.
The genetic basis for addiction to tobacco smoking--particularly that of the perception of olfactory stimuli that may be important in reinforcing smoking addiction--is largely unknown. A cluster of genes for olfactory receptors is in close proximity to the MHC region on chromosome 6. Polymorphisms of MHC class III genes (RCCX modules, TNFA promoter polymorphisms) were determined in 101 healthy subjects and 232 coronary artery disease (CAD) patients from Hungary with defined tobacco smoking habits. A highly significant association between ever smoking (past + current smokers) and a specific MHC haplotype was observed (odds ratios = 2.14-4.13; P-values = 0.012 to <0.001). This haplotype is characterized by the presence of C4A null alleles and a solitary short C4B gene linked to the TNF2 allele of the promoter for TNFA gene. This haplotype occurred more frequently in the ever smokers than in the never smokers [odds ratio: 4.97 (1.96-12.62); P = 0.001], and such associations were stronger in women (odds ratio = 13.6) than in men (odds ratio = 2.79). An independent study of complement C4 protein polymorphism and smoking habits in Icelandic subjects (n = 351) yielded similar and confirmative results. Considering the documented link between olfactory stimuli and smoking in females, and the presence of a cluster of odorant receptor genes close to the MHC class I region, our findings implicate a potential role of the MHC-linked olfactory receptor genes in the initiation of smoking.
Füst
George
G
Third Department of Internal Medicine, Semmelweis University and Hungarian Academy of Sciences, Budapest, Hungary. FustGe@kut.sote.hu
Arason
Gudmundur J
GJ
Kramer
Judith
J
Szalai
Csaba
C
Duba
Jeno
J
Yang
Yan
Y
Chung
Erwin K
EK
Zhou
Bi
B
Blanchong
Carol A
CA
Lokki
Marja-Liisa
ML
Bödvarsson
Sigurdur
S
Prohászka
Zoltán
Z
Karádi
István
I
Vatay
Agnes
A
Kovács
Margit
M
Romics
László
L
Thorgeirsson
Gudmundur
G
Yu
C Yung
CY
D000328:Adult; D015139:Blotting, Southern; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D015934:Complement C4a / Q000235:genetics; D015935:Complement C4b / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics*; D006801:Humans; D006814:Hungary; D007055:Iceland; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018035:Receptors, Odorant / Q000235:genetics; D012737:Sex Factors; D012907:Smoking / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D006649:Histocompatibility Antigens; D018035:Receptors, Odorant; D014409:Tumor Necrosis Factor-alpha; D015934:Complement C4a; D015935:Complement C4b