[Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application].
OBJECTIVE
To establish a new method based DNA chip technique for detecting HCV genotypes.


METHODS
Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.


RESULTS
Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.


CONCLUSIONS
The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.
Mao
Hong-ju
HJ
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Gu
Shi-min
SM
Zhao
Jian-long
JL
Liu
Jiang-xia
JX
D020121:5' Untranslated Regions / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity
D020121:5' Untranslated Regions; D012367:RNA, Viral