title: Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction.
abstract: A simple, rapid, and sensitive analytical method for the measurement of docetaxel in human plasma was developed and validated. The method is based on positive electrospray ionization tandem mass spectrometry (ESI+-MS-MS) with on-line sample extraction. It uses paclitaxel as internal standard for calibration. The on-line sample extraction minimizes sample handling and is readily adopted for automation. Quantitation of plasma docetaxel was done by the multiple reaction monitoring (MRM) mode. The method had a linear calibration range of 1.00-3000 ng/mL with a correlation coefficient >0.9999. The limit of quantitation (LOQ) for docetaxel in plasma was 1.00 ng/mL. The on-line extraction recovery of docetaxel was between 86.1-94.7%, with %CV < or = 6.1%. This method has high accuracy (90.1-96.3%), and excellent intra-assay (0.6-3.8%) and inter-assay (2.0-5.7%) precision. Its applicability to clinical samples was demonstrated by measuring patient plasma samples after treatment of weekly docetaxel at 25 mg/m2 as 60-min infusion.
authors.affiliation: Department of Chemistry, Cleveland State University, 2399 Euclid Avenue, Cleveland, OH 44115, USA.
authors.forename: Adrian G
authors.initials: AG
authors.lastname: Grozav
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hutson
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Bukowski
authors.forename: Ram
authors.initials: R
authors.lastname: Ganapathi
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D002138:Calibration; D000077143:Docetaxel; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D043823:Taxoids / Q000097:blood*; D013997:Time Factors
chemical_list: D043823:Taxoids; D000077143:Docetaxel