[Preimplantation genetic diagnosis of chromosome abnormality by fluorescence in-situ hybridization].
OBJECTIVE
To perform preimplantation genetic diagnosis (PGD) of chromosome abnormality using fluorescence in-situ hybridization (FISH).


METHODS
Ten couples were presented for preimplantation genetic diagnosis. They had a total of 10 oocyte pick-up cycles. The collected oocytes were inseminated by intracytoplasmic sperm injection. PGD was carried out using cleavage-stage (day 3) embryo biopsy, fluorescence in-situ hybridization, and day 4 embryo transfer.


RESULTS
Ten oocyte pick-up cycles yielded 158 oocytes. Among the 94 embryos obtained, 54 embryos were biopsied and FISH analyses were performed for 51 blastomeres. Twenty-four embryos were transferred on the fourth day. There were 4 clinical pregnancies: 3 infants have been born, and 1 couple had ectopic pregnancy.


CONCLUSIONS
PGD is a valuable method to prevent the high risk of spontaneous miscarriages and conceiving chromosomally unbalanced offspring.
Xu
Chen-Ming
CM
Reproductive Medical Center, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Jin
Fan
F
Qian
Yu-Li
YL
Ye
Ying-Hui
YH
Zhu
Yi-Min
YM
Huang
He-Feng
HF
D000022:Abortion, Spontaneous / Q000517:prevention & control; D000328:Adult; D002869:Chromosome Aberrations; D002882:Chromosomes, Human, Pair 13; D002883:Chromosomes, Human, Pair 14; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic