Correlation of cytokine and mobility in mice with arthritis and during therapy with Swertia chirayita.
We attempt to derive a correlation between pro-inflammatory cytokines and mobility in arthritic mice and after treatment with Swertia chirayita plant extract. Tumor necrosis factor-á (TNF-á), interleukin-1alpha (IL-1alpha), interleukin-6 (IL-6) and interleukin-10 (IL-10) were measured by solid phase sandwich ELISA and the different parameters of mobility [distance traveled (DT), resting time (RT), ambulatory time (AT) and stereotypic time (ST)] was monitored and recorded by an Auto track system. Joint circumference of the right hind limb was monitored using a Vernier caliper. Our preliminary findings revealed that DT and RT correlated well with IL-1alpha, TNF-alpha and IL-10 levels in the joint homogenates of arthritic mice and after therapy with Swertia chirayita extracts. AT and ST showed poor correlation with the cytokines. Thus we demonstrate here the anti-inflammatory property of Swertia chirayita and its usage in the prevention of arthritis in the broader aspect in the management of associated cytokines.
Kumar
Pradeep
P
Neurotoxicology Division, Indistrial Toxicology Research Centre, G.M. Marg, P.O. Box 80, Lucknow 226-001, India.
Paul
Bholanath
B
Kumar
Sirish
S
Ali
Mohammad
M
Sexana
Ashok K
AK
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D018836:Inflammation Mediators / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D029967:Swertia; D013583:Synovial Membrane / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D018836:Inflammation Mediators; D007375:Interleukin-1; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10