Crosstalk between elicitor-induced cell death and cell cycle regulation in tobacco BY-2 cells.
The molecular links between cell cycle control and the regulation of programmed cell death are largely unknown in plants. Here we studied the relationship between the cell cycle and elicitor-induced cell death using synchronized tobacco BY-2 cells. Flow cytometry and fluorescence microscopy of nuclear DNA, and RNA gel-blot analyses of cell cycle-related genes revealed that the proteinaceous elicitor cryptogein induced cell cycle arrest at the G1 or G2 phase before the induction of cell death. Furthermore, the patterns of cell death induction and defence-related genes were different in different phases of the cell cycle. Constitutive treatment with cryptogein induced cell cycle arrest and cell death at the G1 or G2 phase. With transient treatment for 2 h, cell cycle arrest and cell death were only induced by treatment with the elicitor during the S or G1 phase. By contrast, the elicitor-induced production of reactive oxygen species was observed during all phases of the cell cycle. These results indicate that although recognition of the elicitor signal is cell cycle-independent, the induction of cell cycle arrest and cell death depends on the phase of the cell cycle.
Kadota
Yasuhiro
Y
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Watanabe
Takashi
T
Fujii
Shinsuke
S
Higashi
Katsumi
K
Sano
Toshio
T
Nagata
Toshiyuki
T
Hasezawa
Seiichiro
S
Kuchitsu
Kazuyuki
K
D016590:Aphidicolin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D010940:Plant Proteins / Q000502:physiology*; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D010940:Plant Proteins; D016590:Aphidicolin