Can a more selective application of antigen retrieval facilitate standardization in immunohistochemistry?
A clearer perception of the major immunochemical forces participating in the reaction between tissue antigens and their complementary antibodies would help us control better the processes of formalin fixation and antigen retrieval. This report describes and discusses the results of additional experiments designed for this purpose. It is anticipated that they will make a useful contribution to the much needed standardization of staining in immunohistochemistry.
Boenisch
Thomas
T
DakoCytomation California, Carpinteria 93013, USA. tom.boenisch@dakocytomation.net
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods* / Q000592:standards; D014066:Palatine Tonsil / Q000276:immunology; D012015:Reference Standards; D013194:Staining and Labeling; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000941:Antigens