Analytical performance of and real sample analysis with an HBV gene visual detection chip.
A novel hepatitis B virus (HBV) gene detection chip has been developed. The HBV-specific probes immobilized on glass slides were hybridized with polymerase chain reaction (PCR) products of different serum samples. The hybridization signal can be easily visualized upon a sandwich assay with nanoparticle amplification. The analytical performance (e.g., specificity, sensitivity, and accuracy) of this method has been evaluated. The chip-based detection method possesses a greater sensitivity and a better reproducibility than some of the conventional immunological or molecular biological methods (e.g., enzyme-linked immunosorbent assay, ELISA) and is simple, cost-effective, and highly selective.
Wang
Ye-Fu
YF
College of Life Sciences, Wuhan University, Wuhan 430072, PR China. wangyf25@sohu.com
Shen
Jun-Tao
JT
Liu
Hui-Hui
HH
D004279:DNA, Viral / Q000097:blood*; D005814:Genes, Viral; D017979:Gold Colloid; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D049329:Nanostructures; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver
D004279:DNA, Viral; D017979:Gold Colloid; D012834:Silver