Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution.
The mechanical rigidity and degradation rate of hydrogels utilized as cell transplantation vehicles have been regarded as critical factors in new tissue formation. However, conventional approaches to accelerate the degradation rate of gels deteriorate their function as a mechanical support in parallel. We hypothesized that adjusting the molecular weight distribution of polymers that are hydrolytically labile but capable of forming gels would allow one to alter the degradation rate of the gels over a broad range, while limiting the range of their elastic moduli (E). We investigated this hypothesis with binary alginate hydrogels formed from both ionically and covalently cross-linked partially oxidized (1% uronic acid residues), low [molecular weight (MW) approximately 60,000 g/mol] and high MW alginates (MW approximately 120,000 g/mol) in order to examine the utility of this approach with various cross-linking strategies. Increasing the fraction of low MW alginates to 0.50 maintained a value of E similar to that for the high MW alginate gels but led to faster degradation, irrespective of the cross-linking mode. This result was attributed to a faster separation between cross-linked domains upon chain breakages for the low MW alginates, coupled with their faster chain scission than the high MW alginates. The more rapidly degrading oxidized binary hydrogels facilitated the formation of new bone tissues from transplanted bone marrow stromal cells, as compared with the nonoxidized high MW hydrogels. The results of these studies will be useful for controlling the physical properties of a broad array of hydrogel-forming polymers.
Kong
Hyun Joon
HJ
Department of Biologic and Materials Science, University of Michigan, Ann Arbor 48109-2136, USA.
Kaigler
Darnell
D
Kim
Kibum
K
Mooney
David J
DJ
D000464:Alginates / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D008970:Molecular Weight; D051381:Rats
D000464:Alginates; D020100:Hydrogels