Exogenous application of glucose induces aging in rat cerebral oligodendrocytes as revealed by alteration in telomere length.
To investigate aspects of aging on rat oligodendrocytes, cells of an oligodendrocyte cell line, so-called OLN-93, were cultured either in the presence or absence of glucose. Our data demonstrated that glucose-induced aging in vitro caused an elongation and thickening of cell processes and significantly increased the expression of netrin reflecting a more mature state of oligodendrocyte development. A possible age-inducing effect of glucose is also supported by the decrease of ras protein expression and shortening of telomeres in glucose-treated oligodendrocytes. The present study clearly shows that OLN-93 cells are an exciting and suitable model system for the investigation of age-inducing molecules and the analysis of signaling pathways involved in cerebral aging and degenerations.
Dabouras
Vasili
V
Department of Chemistry and Applied Science, Institute of Pharmaceutical Science, Swiss Federal, Institute of Technology, Zürich, Switzerland.
Rothermel
Andrée
A
Reininger-Mack
Alexandra
A
Wien
Sascha L
SL
Layer
Paul Gottlob
PG
Robitzki
Andrea Anneliese
AA
D000375:Aging / Q000187:drug effects*; D000818:Animals; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D003470:Culture Media; D004247:DNA / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000378:metabolism; D000075388:Netrin-1; D009836:Oligodendroglia / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016615:Telomere / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D025521:Tumor Suppressor Proteins; D018631:ras Proteins / Q000378:metabolism
D003470:Culture Media; D009414:Nerve Growth Factors; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1; D004247:DNA; D018631:ras Proteins; D005947:Glucose