[Using recombinant antigens of Hantavirus to study the kinetics of serum IgA, IgG, IgM antibodies in the acute-phase of hemorrhagic fever renal syndrome].
BACKGROUND
To observe the features of serum specific IgA, IgG, IgM antibodies in the acute phase of hemorrhagic fever renal syndrome (HFRS).


METHODS
The nucleocapsid (NP) protein and glycoproteins (GP) of Hantavirus were expressed by recombinant baculovirus, and used as ELISA antigens to test 61 serial sera of 14 acute phase HFRS patients.


RESULTS
Seoul like virus RNA were detected from 11 of 14 patients. An early and strong IgA, IgG and IgM antibody response to recombinant NP (rNP) was observed in almost all HFRS cases. The titers of antibody to rNP was apparently higher than that to Rgp. In the early stage, titer of IgG antibody elevated most drastically among all the three classes of antibodies to rNP, followed by IgM and IgA antibody responses. The elevation trend of IgM and IgA antibodies to rNP stayed nearly at the same level, but the IgA titers to rNP were apparently higher than that of IgM. Among the antibodies to rGP, IgA changed distinctly greater than IgG. The elevation trend of IgM could be found during first week after the onset, and the titers dropped gradually after the second week. IgM antibodies of one case who was viral RNA positive were not detected at early stage, but IgA titers were high. The only severe case of the 14 patients kept the lower IgA, IgG and IgM during the whole acute phase.


CONCLUSIONS
HFRS patients kept an early and strong humoral response to NP and GPs in acute phase of HFRS.IgA could be used together with IgM to improve the diagnostic accuracy.
Meng
Xiang-shi
XS
Institute of Viral Diseases Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 100052, China.
Chen
Yu-ping
YP
Li
Chuan
C
Yu
Jian-shi
JS
Guo
Yan-min
YM
Zhang
Quan-fu
QF
Sun
Yu-lan
YL
Li
De-xin
DX
D000208:Acute Disease; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D005260:Female; D006476:Orthohantavirus / Q000235:genetics / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology* / Q000821:virology; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000097:blood*; D008297:Male; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007132:Immunoglobulin Isotypes; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; C111782:nucleocapsid protein, Hantaan virus