[Cellular ultrastructural changes of bone marrow of patients with hemorrhagic fever with renal syndrome].
BACKGROUND
To observe cytopathogenic effect of Hantaan virus (HV) on cultured human bone marrow cells.


METHODS
Light and transmission electron microscopy and direct immunofluorescent technique were applied to study cellular structure especially ultrastructural changes of bone marrow cells from patients with Hantaan virus infection. Bone marrow cells of one healthy volunteer were also studied as control.


RESULTS
The antigen of HV was found in bone marrow cells of 20 of 27 HFRS patients by the aid of direct immunofluorescent technique. It was found that the granulocytes had the highest percentage of HV antigen positive cells (76%), followed by monocytes (65%), lymphocytes (40%), megakaryocytes (20%) and the lowest was found in erythrocytes (3.7%). The injury of cell membrane after infection with HV was significantly more severe than that in the control group under the light and electron microscopy.


CONCLUSIONS
This study demonstrated that HV could attack human bone marrow cells and cause cytopathogenic effect on them.
Liang
Ke-shan
KS
The People's Hospital of Pingyi County, Shandong Province 273300, China.
Peng
Li-jing
LJ
Yin
Cheng-bin
CB
Zhang
Jing-ling
JL
Xu
Cong-gao
CG
Liu
Xiang-dong
XD
Du
Jing-yun
JY
Chen
Wei-ning
WN
D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001854:Bone Marrow Cells / Q000648:ultrastructure* / Q000821:virology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006476:Orthohantavirus / Q000276:immunology / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000473:pathology* / Q000821:virology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008875:Middle Aged
D000956:Antigens, Viral