title: Presynaptic dopaminergic properties of differentiated mouse embryonic stem cells.
abstract: This study characterized the presynaptic dopaminergic properties of neuronally differentiated mouse embryonic stem (ES) cells. Approximately 30% of the ES cells expressed tyrosine hydroxylase (TH) immunoreactivity when co-cultured with PA6 cells. These cultures expressed high affinity, sodium-dependent dopamine uptake as well as depolarization-induced and calcium-dependent dopamine release of this transmitter. These and other important dopaminergic genes found expressed in these cultures by RT-PCR included Nurr1, vesicular monoamine transporter 2 (VMAT2), TH, dopamine transporter (DAT), and glial cell line-derived neurotrophic factor (GDNF) receptors c-Ret and GFRalpha1. These results demonstrate that differentiated ES cells have the presynaptic functions for maintaining dopaminergic homeostasis, which may be essential for their long-term use in restoring CNS levels of this transmitter.
authors.affiliation: Department of Pharmacology, College of Pharmacy, University of Florida, P.O. Box 100267, 1600 SW Archer, Gainesville, FL 32610, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: A C
authors.initials: AC
authors.lastname: Hirko
authors.forename: A D
authors.initials: AD
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: R
authors.initials: R
authors.lastname: Dawson
authors.forename: J
authors.initials: J
authors.lastname: Peris
authors.forename: N
authors.initials: N
authors.lastname: Terada
authors.forename: E M
authors.initials: EM
authors.lastname: Meyer
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D004298:Dopamine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004622:Embryo, Mammalian; D051379:Mice; D017729:Presynaptic Terminals / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D004298:Dopamine