title: [Cellular ultrastructural changes of bone marrow of patients with hemorrhagic fever with renal syndrome].
abstract: BACKGROUND
  To observe cytopathogenic effect of Hantaan virus (HV) on cultured human bone marrow cells.
  
  
  METHODS
  Light and transmission electron microscopy and direct immunofluorescent technique were applied to study cellular structure especially ultrastructural changes of bone marrow cells from patients with Hantaan virus infection. Bone marrow cells of one healthy volunteer were also studied as control.
  
  
  RESULTS
  The antigen of HV was found in bone marrow cells of 20 of 27 HFRS patients by the aid of direct immunofluorescent technique. It was found that the granulocytes had the highest percentage of HV antigen positive cells (76%), followed by monocytes (65%), lymphocytes (40%), megakaryocytes (20%) and the lowest was found in erythrocytes (3.7%). The injury of cell membrane after infection with HV was significantly more severe than that in the control group under the light and electron microscopy.
  
  
  CONCLUSIONS
  This study demonstrated that HV could attack human bone marrow cells and cause cytopathogenic effect on them.
authors.affiliation: The People's Hospital of Pingyi County, Shandong Province 273300, China.
authors.forename: Ke-shan
authors.initials: KS
authors.lastname: Liang
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Peng
authors.forename: Cheng-bin
authors.initials: CB
authors.lastname: Yin
authors.forename: Jing-ling
authors.initials: JL
authors.lastname: Zhang
authors.forename: Cong-gao
authors.initials: CG
authors.lastname: Xu
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Jing-yun
authors.initials: JY
authors.lastname: Du
authors.forename: Wei-ning
authors.initials: WN
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001854:Bone Marrow Cells / Q000648:ultrastructure* / Q000821:virology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006476:Orthohantavirus / Q000276:immunology / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000473:pathology* / Q000821:virology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008875:Middle Aged
chemical_list: D000956:Antigens, Viral