Performance of quantitative analyses by liquid chromatography-electrospray ionisation tandem mass spectrometry: from external calibration to isotopomer-based exact matching.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a versatile coupling system which combines both selectivity and sensitivity and certainty. Hence, it is generally considered as the most reliable technique to quantify chemical compounds in complex matrices. In the present paper, we evaluate the performance of LC-MS/MS methods for the quantification of 3-nitrotyrosine in human urine in order to point out its dependence on the design of the quantification method, and emphasize the role of matrix effects in the performance. We compare external and internal calibrations, isotope dilution and isotopomer-based exact matching. The role of both sample preparation and multiple transitions monitoring is particularly addressed.
Delatour
Thierry
T
Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, 1000, Lausanne 26, Switzerland. thierry.delatour@rdls.nestle.com
D002138:Calibration; D011232:Chemical Precipitation; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000652:urine
D011506:Proteins; C002744:3-nitrotyrosine; D014443:Tyrosine