Use of surface-enhanced laser desorption/ionization -time of flight to explore bacterial proteomes.
Surface-enhanced laser desorption/ionization (SELDI)-time of flight is a recent technology that allows proteomic analysis with limited material requirements. This characteristic makes it a valuable technique for microbiologists handling problematic samples, such as low cell number cultures. We compared three simple procedures for protein extraction from bacteria for compatibility with the ProteinChip Array; we also determined the amount of protein required for each analysis. The protocol for the SELDI analysis was evaluated by generating protein expression profiles of a Streptococcus pneumoniae strain grown in different conditions and those of different strains of the same species. The protocol also was successfully applied to a wide range of Gram positive and negative bacteria. The results of this study suggest the appropriateness of this technology for microorganism protein profiling as complementary or alternative to two-dimensional gel electrophoresis.
Barzaghi
Dagania
D
Department of Molecular and MicroBiology, George Mason University, Manassas, VA 20110, USA.
Isbister
Jenefir D
JD
Lauer
Kimberly P
KP
Born
Timothy L
TL
D001426:Bacterial Proteins / Q000032:analysis*; D020869:Gene Expression Profiling; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000737:chemistry
D001426:Bacterial Proteins; D020543:Proteome