title: Conjugation of a novel histidine derivative to biomolecules and labelling with [99mTc(OH2)3(CO)3]+.
abstract: The new histidine derivative 3-[1-[3-(9H-fluoren-9-ylmethoxycarbonylamino)-propyl]-1H-imidazol-4-yl]-2-(3-trimethylsilanyl-ethylcarboxyamino)-propionic acid methyl ester (7) has been prepared via alkylation of the histidine urea derivative (7S)-5,6,7,8-tetrahydro-7-(methoxycarbonyl)-5-oxoimidazo-[1,5-c]-pyrimidine (2) with Fmoc-protected 3-iodopropyl-amine, followed by ring opening with 2-trimethylsilylethanol. After Fmoc cleavage by HNEt2, the histidine amine derivative was coupled to biotin, to the pentapeptide leucine-enkephalin and to Vitamin B12-b-acid by amide formation, employing TBTU as the coupling reagent. In order to make the histidine accessible for labelling, the teoc protecting group was removed by either NBu4F (for the biotin conjugate) or by TFA (for the enkephalin and B12 conjugates). Reaction of a 10(-4) M solution of the bioconjugates with [99mTc(H2O)3(CO)3]+ at 50 degrees C for 30 min led to the formation of one single new peak in the HPLC radiochromatogram in each case, confirming quantitative labelling of the respective biomolecules. To assess the nature of the labelled compounds, the rhenium analogues with Re(CO)3 were also synthesised and similar retention times confirmed the identity with the 99mTc labelled conjugates.
authors.affiliation: Institute of Inorganic Chemistry, University of Zurich, Wintherturerstrasse 190, CH-8057, Zurich, Switzerland.
authors.forename: Dave R
authors.initials: DR
authors.lastname: van Staveren
authors.forename: Stefan
authors.initials: S
authors.lastname: Mundwiler
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hoffmanns
authors.forename: Jae Kyoung
authors.initials: JK
authors.lastname: Pak
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spingler
authors.forename: Nils
authors.initials: N
authors.lastname: Metzler-Nolte
authors.forename: Roger
authors.initials: R
authors.lastname: Alberto
mesh_terms: D001710:Biotin / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004743:Enkephalin, Leucine / Q000737:chemistry*; D006639:Histidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D007553:Isotope Labeling; D008958:Models, Molecular; D015394:Molecular Structure; D015609:Organotechnetium Compounds / Q000737:chemistry*; D014805:Vitamin B 12 / Q000737:chemistry*
chemical_list: D015609:Organotechnetium Compounds; D006639:Histidine; D004743:Enkephalin, Leucine; D001710:Biotin; D014805:Vitamin B 12