Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells.
Generally, heat inactivated foetal calf serum (FCS) containing media are used for the cultivation of animal and human cells. The role of serum source and serum treatment on the behaviour of cells has long been neglected. The present study was performed to investigate the effects of serum heat inactivation and serum source on trabecular bone derived progenitor cells (HBC). Furthermore, it was investigated in how far these reactions differed from those seen in bone marrow derived mesenchymal progenitor cells (HBMC) cultures. We found that HBC cultures performed differently in the presence of FCS and HS with or without heat inactivation. The reactions similar to some degree those observed in HBMC cultures. The implications of the results on cell-implant surface interaction studies are discussed.
Bruinink
A
A
MaTisMed, Biocompatible Materials, Swiss Federal Laboratories for Material Testing and Research (EMPA), Lerchenfeldstrasse 5, CH-9014 St Gallen, Switzerland. arie.bruinink@empa.ch
Tobler
U
U
Hälg
M
M
Grünert
J
J
D000368:Aged; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D044967:Serum / Q000378:metabolism* / Q000528:radiation effects*; D013242:Sterilization / Q000379:methods