[Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice].
BACKGROUND
Construction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.


METHODS
gag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.


RESULTS
The replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.


CONCLUSIONS
Replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.
Liu
Ying
Y
National Center for AIDS Prevention and Control. Center for Disease Control and Prevention, Beijing 100050, China.
Wu
Lan
L
Zhou
Ke-ming
KM
Zhang
Xu-dong
XD
Hong
Kun-sue
KS
Shao
Yi-ming
YM
D016915:AIDS Vaccines / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000235:genetics*; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D014162:Transfection; D019444:Vaccines, DNA / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D015685:Fusion Proteins, gag-pol; D015686:Gene Products, env; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1