title: Fluorescence-based on-line detection as an analytical tool in RNA electrophoresis.
abstract: In this chapter, we present methods for denaturing and native gel electrophoresis of nucleic acids based on fluorescently labeled probes and automatic signal detection by a deoxyribonucleic acid sequencer. Specific examples are given for the determination of ribonucleic acid (RNA) fragmentation patterns, of (deoxy)ribozyme kinetics, and of direct or competitive gel-shift assays. These methods can replace widely used radioisotope-based protocols, for example, for secondary structure mapping of RNA and for the characterization of nucleic acid ligand interactions.
authors.affiliation: Department of Organic Chemistry and Cheical Biology, Johann Wolfgang Goethe-University Frankfurt, Franfurt am Main, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Scheffer
authors.forename: Michael
authors.initials: M
authors.lastname: Göbel
mesh_terms: D001483:Base Sequence; D004586:Electrophoresis; D007700:Kinetics; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D012313:RNA