Toxoplasma gondii-derived heat shock protein 70 stimulates the maturation of human monocyte-derived dendritic cells.
We investigated the role of Toxoplasma gondii-derived heat shock protein 70 (TgHSP70) as a dendritic cell (DC) maturation-inducing molecule. TgHSP70 induced the maturation of human monocyte-derived dendritic cells as determined by increased levels of surface markers, namely, CD40, CD80, CD86, and HLA-DR. Moreover, TgHSP70 also reduced phagocytic activity and increased the allostimulatory capacity of DCs, suggesting the functional maturation of DCs by TgHSP70. Maturation of DCs by TgHSP70 also elicited a significant increase in IL-12 production in a polymyxin B-insensitive manner. TgHSP70 also stimulated extracellular signal-regulated kinase and p38 kinase pathways in DCs, and TgHSP70-induced IL-12 production was inhibited by SB203580 but not by PD98059, thus indicating the role of p38 kinase in the maturation of DCs by TgHSP70. This study demonstrates the role of TgHSP70 in the functional maturation of DCs and suggests TgHSP70 as a useful molecule for the development of a vaccine against T. gondii.
Kang
Hyun Kyu
HK
Medical Research Center for Cancer Molecular Therapy, Dong-A University, Busan 602-714, Republic of Korea.
Lee
Ha-Young
HY
Lee
Youl-Nam
YN
Jo
Eun Jin
EJ
Kim
Jung Im
JI
Aosai
Fumie
F
Yano
Akihiko
A
Kwak
Jong-Young
JY
Bae
Yoe-Sik
YS
D000818:Animals; D015703:Antigens, CD / Q000097:blood; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D015800:Protozoan Proteins / Q000494:pharmacology*; D014122:Toxoplasma / Q000502:physiology*
D015703:Antigens, CD; D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D015800:Protozoan Proteins