title: Serological response to Pasteurella multocida NanH sialidase in persistently colonized rabbits.
abstract: Pasteurella multocida is a mucosal pathogen that colonizes the upper respiratory system of rabbits. Respiratory infections can result, but the bacteria can also invade the circulatory system, producing abscesses or septicemia. P. multocida produces extracellular sialidase activity, which is believed to augment colonization of the respiratory tract and the production of lesions in an active infection. Previously, it was demonstrated that some isolates of P. multocida contain two unique sialidase genes, nanH and nanB, that encode enzymes with different substrate specificities (S. Mizan, A. D. Henk, A. Stallings, M. Meier, J. J. Maurer, and M. D. Lee, J. Bacteriol. 182:6874-6883, 2000). We developed a recombinant antigen enzyme-linked immunosorbent assay (ELISA) based on the NanH sialidase of P. multocida and demonstrated that rabbits that were experimentally colonized with P. multocida produce detectable anti-NanH immunoglobulin M (IgM) and IgG in serum, although they demonstrated no clinical signs of pasteurellosis. In addition, clinically ill pet rabbits infected with P. multocida possessed IgM and/or IgG antibody against NanH. The NanH ELISA may be useful for the diagnosis of P. multocida infections in sick rabbits as well as for screening for carriers in research rabbit colonies.
authors.affiliation: Athens Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Sanchez
authors.forename: Shaikh
authors.initials: S
authors.lastname: Mizan
authors.forename: Charlotte
authors.initials: C
authors.lastname: Quist
authors.forename: Patricia
authors.initials: P
authors.lastname: Schroder
authors.forename: Michelle
authors.initials: M
authors.lastname: Juneau
authors.forename: Donald
authors.initials: D
authors.lastname: Dawe
authors.forename: Branson
authors.initials: B
authors.lastname: Ritchie
authors.forename: Margie D
authors.initials: MD
authors.lastname: Lee
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D009439:Neuraminidase / Q000276:immunology*; D010326:Pasteurella Infections / Q000175:diagnosis*; D016979:Pasteurella multocida / Q000201:enzymology*; D011817:Rabbits; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C500021:NanH sialidase, Pasteurella multocida; D009439:Neuraminidase