title: Cell shape regulation and co-translocation of actin and adenosyl homocysteinase in response to intermediate hypertonicity.
abstract: Hypertonic stimulation induced association of S-adenosyl-L-homocysteine hydrolase (SAHH) with the F-actin-rich cell cortex in Dictyostelium. At intermediate, but not higher, levels of hypertonicity, SAHH further translocated from the cortex to the cytosol in company with a fraction of actin and cofilin. At the same time the cells rounded up. Acidification of the cells stimulated both the cell rounding and the translocation of actin and SAHH, whereas alkalinization retarded these responses, suggesting that cellular pH is involved in their control. On the other hand, mutant analysis suggested that neither cGMP signaling nor conventional myosin is required.
authors.affiliation: Electron Microscopy Center, The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan. yyamada@chaos.bio.sci.osaka-u.ac.jp
authors.forename: Yohko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Masazumi
authors.initials: M
authors.lastname: Sameshima
mesh_terms: D000199:Actins / Q000378:metabolism*; D043383:Adenosylhomocysteinase / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006863:Hydrogen-Ion Concentration; D006982:Hypertonic Solutions / Q000494:pharmacology*; D021381:Protein Transport / Q000502:physiology
chemical_list: D000199:Actins; D006982:Hypertonic Solutions; D043383:Adenosylhomocysteinase