title: Effects of Zuogui Wan on neurocyte apoptosis and down-regulation of TGF-beta1 expression in nuclei of arcuate hypothalamus of monosodium glutamate -liver regeneration rats.
abstract: OBJECTIVE
  To inquire into the effects and mechanism of Zuogui Wan (Pills for Kidney Yin) on neurocyte apoptosis in nuclei of arcuate hypothalamus (ARN) of monosodium glutamate (MSG)-liver regeneration rats, and the mechanism of liver regeneration by using optic microscope, electron microscope and in situ end labeling technology to adjust nerve-endocrine-immunity network.
  
  
  METHODS
  Neurocyte apoptosis in ARN of the experiment rats was observed by using optic microscope, electron microscope and in situ end labeling technology. Expression of TGF-beta1 in ARN was observed by using immunohistochemistry method.
  
  
  RESULTS
  The expression of TGF-beta1 in rats of model group was increased with the increase of ARN neurocyte apoptosis index (AI) (t = 8.3097, 12.9884, P<0.01). As compared with the rats of model group, the expression of TGF-beta1 in rats of Zuogui Wan treatment group was decreased with the significant decrease of ARN neurocyte apoptosis (t = 4.5624, 11.1420, P<0.01).
  
  
  CONCLUSIONS
  Brain neurocyte calcium ion overexertion and TGF-beta1 protein participate in the adjustment and control of ARN neurocyte apoptosis in MSG-liver regeneration-rats. Zuogui Wan can prevent ARN neurocyte apoptosis of MSG-liver regeneration in rats by down-regulating the expression of TGF-beta1, and influence liver regeneration through adjusting nerve-endocrine-immune network.
authors.affiliation: Hepatopathy Institute, Affiliated Hospital of Hubei Traditional Chinese Medicine College, Wuhan 430061, Hubei Province, China.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Mu-Lan
authors.initials: ML
authors.lastname: Yang
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Mei
authors.forename: Liu-Tong
authors.initials: LT
authors.lastname: Zhang
authors.forename: Xing-Fan
authors.initials: XF
authors.lastname: Qiu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000502:physiology*; D002467:Cell Nucleus / Q000187:drug effects / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1
chemical_list: D004365:Drugs, Chinese Herbal; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D012970:Sodium Glutamate