[Capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum].
BACKGROUND
To develop a capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.


METHODS
The anti-HCMV-IgM antibody was detected in 68 patients with HCMV infection by the capturing-ELISA, and the results were compared with those of indirect ELISA.


RESULTS
The specificity and sensitivity of the capturing-ELISA were shown to be significantly higher than those of indirect ELISA, and its results were not affected by RF factor.


CONCLUSIONS
The capturing ELISA is specific, sensitive, convenient and reliable method which may be feasible for clinical use.
Zhang
Xin-ping
XP
Laboratory of Medical Technology, Hangzhou Sixth People's Hospital, Zhejiang Province, Hangzhou 310014, China.
Li
Pei
P
Liu
Hong-jing
HJ
Liu
Bao-gen
BG
D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*
D000914:Antibodies, Viral; D007075:Immunoglobulin M