Rubus croceacanthus Leveille inhibits mast cell-mediated anaphylactic-like reaction and tumor necrosis factor-alpha secretion.
This work aims at examining the effect of the concentrated methanol extract of Rubus croceacanthus Leveille (RCL) on mast cell-mediated anaphylactic-like reaction in a murine model. RCL inhibited compound 48/80-induced systemic anaphylactic-like reaction. When RCL was given as pre-treatment at concentrations ranging from 0.01 to 1 mg/ml, the histamine release from rat peritoneal mast cells induced by compound 48/80 or anti-dinitrophenyl (DNP) immunoglobulin E (IgE) was reduced in a dose-dependent manner. RCL also inhibited passive cutaneous anaphylaxis activated by anti-DNP IgE. In addition, RCL inhibited phorbol 12-myristate 13-acetate and A23187-induced tumor necrosis factor-alpha secretion from human mast cell line HMC-1 cells. These results indicate that RCL may possess a strong anti-anaphylactic activity.
Moon
Phil-Dong
PD
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea.
Choi
In-Young
IY
Na
Ho-Jeong
HJ
Jeong
Hyun-Ja
HJ
Kim
Cheorl-Ho
CH
Kim
Hyung-Ryong
HR
Kim
Yun-Kyung
YK
Park
Seong Kyu
SK
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control; D000818:Animals; D002460:Cell Line; D006632:Histamine / Q000378:metabolism; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027824:Rosaceae / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine