Identification of TPO receptors on central nervous system-a preliminary report.
To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.
Yang
Mo
M
Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong. yang1091@cuhk.edu.hk
Xia
Wen-Jie
WJ
Li
Karen
K
Pong
Nga-Hin
NH
Chik
Ki-Wai
KW
Li
Chi-Kong
CK
Ng
Margaret H L
MH
Ng
Ho-Keung
HK
Fung
Kwok-Pui
KP
Fok
Tai-Fai
TF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001923:Brain Chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis*; D009474:Neurons / Q000187:drug effects; D015513:Oncogene Proteins / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D018121:Receptors, Cytokine / Q000032:analysis*; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin / Q000494:pharmacology
C481270:Mpl protein, mouse; D009363:Neoplasm Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; D004921:Erythropoietin; C082218:MPL protein, human; D013926:Thrombopoietin