title: Absorption of D(+)-xylose, cobalamin, and folic acid after autologic-allotopic ileum mucosa transplantation in beagles.
abstract: OBJECTIVE
  Ileum mucosa transplantation in a demucosed colon coat was developed as a new method for small bowel elongation. In an animal model, the authors investigated the absorptive capacity of the transplanted mucosa for D(+)-xylose, cobalamin (vitamin B12), and folic acid.
  
  
  METHODS
  Ileum mucosa was transplanted in a vascularized demucosed segment of transverse colon in 18 beagle dogs. The colon coat-ileum mucosa complex then was integrated in the ileal continuity. Absorptive capacity for D(+)-xylose, cobalamin, and folic acid was measured before and 4 weeks after transplantation. The results were compared and analyzed with the Students' t test for matched pairs. All determined blood values with P values less than.05 were considered to show a significant reduction in the absorptive capacity of the transplanted ileum mucosa.
  
  
  RESULTS
  Fifteen minutes after application there was no significant difference in the absorption of D(+)-Xylose and cobalamin between normal and transplanted ileum mucosa (P >.1). Absorption of folic acid in the transplanted segment was lower but not significant (P <.1). After 30 minutes D(+)-xylose and cobalamin again showed no difference between the absorptive capacity of normal and transplanted ileum mucosa (P >.1), whereas folic acid continued with the tendency toward an impaired absorption (P <.1). However, after 60 minutes, the difference of the absorptive capacity of the transplanted ileum mucosa was significant (P <.05) for folic acid. D(+)-xylose showed a tendency for an impaired uptake (P <.1), whereas absorption of cobalamin did not differ significantly after transplantation (P >.1).
  
  
  CONCLUSIONS
  Experimental autologic-allotopic ileum mucosa transplantation is a feasible new method for small bowel elongation in an animal model. Examination of the absorptive capacity of the transplanted ileum mucosa showed a normal uptake for cobalamin, while there was an impaired absorption of D(+)-xylose and folic acid.
authors.affiliation: Department of Pediatric Surgery, University of Heidelberg, Heidelberg, Germany.
authors.forename: H A
authors.initials: HA
authors.lastname: Beiler
authors.forename: J
authors.initials: J
authors.lastname: Steinorth
authors.forename: A
authors.initials: A
authors.lastname: Witt
authors.forename: M
authors.initials: M
authors.lastname: Aulmann
authors.forename: Z
authors.initials: Z
authors.lastname: Zachariou
mesh_terms: D000818:Animals; D003106:Colon / Q000601:surgery*; D004285:Dogs; D005492:Folic Acid / Q000097:blood / Q000493:pharmacokinetics*; D007082:Ileum / Q000637:transplantation*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000637:transplantation*; D011184:Postoperative Period; D012778:Short Bowel Syndrome / Q000517:prevention & control; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic; D014805:Vitamin B 12 / Q000097:blood / Q000493:pharmacokinetics*; D014994:Xylose / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D005492:Folic Acid; D014994:Xylose; D014805:Vitamin B 12