Phenotypic and genotypic characterization of beta-D-glucuronidase-positive Shiga toxin-producing Escherichia coli O157:H7 isolates from deer.
Beta-glucuronidase-positive (GUD+) Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from both an asymptomatic woman and uncooked deer meat in her possession in Hokkaido, Japan. The phenotypic and genotypic characteristics of the two isolates were identical or closely related, indicating probable transmission of the deer isolate to the woman. Moreover, several other GUD+ STEC O157:H7 strains investigated belonged to the distinct atypical GUD+ STEC O157:H7 group that has been identified previously. This is the first report that deer can be a reservoir of GUD+ STEC O157:H7 in Japan.
Nagano
Hideki
H
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
Hirochi
Takashi
T
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
Fujita
Kozo
K
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
Wakamori
Yoshihiro
Y
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
Takeshi
Koichi
K
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
Yano
Shoki
S
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan 2Sapporo City Institute of Public Health, Kikusui 9-1, Shiroishi-ku, Sapporo 003-8505, Japan.
D000818:Animals; D003670:Deer / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D005966:Glucuronidase / Q000032:analysis*; D006801:Humans; D010641:Phenotype; D022621:Shiga Toxin / Q000096:biosynthesis*
D022621:Shiga Toxin; D005966:Glucuronidase