title: Severity of viral infection is promoted by hyperthermic pretreatment.
abstract: The aim of this study was to determine if prior heat shock and consequential heat shock protein (HSP) accumulation would inhibit viral infection. Confluent cultures of Madin-Darby bovine kidney cells were subjected to 30 mins of hyperthermia at 42 degrees C then allowed to recover at 37 micro C for six hrs. During the six hrs, HSP concentrations increased by 232% in heat-shocked cultures. Following the recovery period, six replicates of heat-shocked (HS) and six replicates of non-heat-shocked (CON) cultures were infected with Bovine Herpes virus 1. After 72 hours, severity of infection was assessed by counting formed viral plaques in cell cultures. CON cultures averaged 172.0+/-34.3 plaques per culture and HS cultures averaged 339.3+/-96.1 plaques per culture, representing a significant increase in infected cells (p=0.0044). These data provide evidence that physiological stressors related to extreme or exhaustive exercise, specifically hyperthermia, may increase risk of viral infection.
authors.affiliation: Exercise Biochemistry Laboratory, Department of Kinesiology, College of Health Sciences and Human Services, Midwestern State University, Wichita Falls, Texas, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Kilgore
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000378:metabolism* / Q000821:virology*; D007242:Herpesvirus 1, Bovine; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D007668:Kidney / Q000166:cytology / Q000821:virology; D012016:Reference Values
chemical_list: D006360:Heat-Shock Proteins