CDNA cloning and characterization of the Ve homologue gene StVe from Solanum torvum Swartz.
Verticillium wilt is a disastrous disease causing significant yield losses of many crops. Isolation of verticillium wilt resistance gene is a fundamental work for controlling this disease through genetic engineering. In this report, we describe the cloning and characterization of a Ve like gene (StVe) from Solanum torvum Swartz. The nucleotide sequence of StVe is 3640 bp long with an open reading frame of 3414 bp encoding a protein precursor of 1138 aa. Sharing high homologies to tomato verticillium wilt disease resistance genes Ve1 and Ve2, the leucine rich (15.89%) protein StVe has a calculated molecular weight of 126.48kDa with an isoelectric point of 5.62. It possesses a hydrophobic N-terminal signal peptide of 20 aa and 38 predicted leucine-rich repeats containing 32 potential N-glycosylation sites (28 being significant). Fifty-seven predicted phosphorylation sites (36 for S, 8 for T and 13 for Y) distribute in StVe protein. A PEST-like sequence and a mammalian endocytosis signals YCVF are found within the C-terminal region. The C terminus of StVe concludes with the residues KKF similar to the KKX motif that confers endoplasmic reticulum localization in plants as well as mammals and yeast. The sequence analysis of the StVe gene implies that the StVe is a potential verticillium wilt disease resistance gene encoding a cell surface-like receptor protein.
Fei
Jiong
J
Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China.
Chai
Yourong
Y
Wang
Jin
J
Lin
Juan
J
Sun
Xiaofen
X
Sun
Chao
C
Zuo
Kaijing
K
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D006023:Glycoproteins / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011956:Receptors, Cell Surface / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D032322:Solanum / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C430462:Ve1 protein, Lycopersicon esculentum; C430463:Ve2 protein, Lycopersicon esculentum