title: Cellular investigations on electrochemically deposited calcium phosphate composites.
abstract: Electrochemically deposited calcium phosphate (CaP) coatings are fast resorbable and existent only during the first period of osseointegration. In the present study, composite coatings with varying solubility (hydroxyapatite (HA), brushite with less HA and monetite (M) with less HA) were prepared and the influence of the degradation and the reprecipitation of CaP on osteoblastic cells were investigated. On the brushite composite coating a new precipitated, finely structured CaP phase was observed during immersion in cell culture medium with or without osteoblastic cells. The surface morphology of monetite and HA coatings were entirely unmodified under the same conditions. So it could be assumed that electrochemically deposited brushite with less HA acts as a precursor for new precipitated CaP. On this surface osteoblastic cells revealed a well-spread morphology with pronounced actin cytoskeleton and demonstrated good proliferation behaviour. Thus we suggest that brushite seems to be especially suitable for coating of implants as a matrix for nucleation and growth of new bone.
authors.affiliation: DOT GmbH, Charles-Darwin-Ring 1a, 18059 Rostock, Germany. becker@dot-coating.de
authors.forename: Petra
authors.initials: P
authors.lastname: Becker
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Neumann
authors.forename: Barbara
authors.initials: B
authors.lastname: Nebe
authors.forename: Frank
authors.initials: F
authors.lastname: Lüthen
authors.forename: Joachim
authors.initials: J
authors.lastname: Rychly
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D002130:Calcium Phosphates / Q000737:chemistry*; D002455:Cell Division; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D008420:Manufactured Materials / Q000032:analysis; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D014025:Titanium; C494366:calcium phosphate, dibasic, dihydrate