title: Expression and characterization of cholera toxin B-pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTB-PsaA to induce humoral immune response in mice.
abstract: Cholera toxin B subunit (CTB) is responsible for CT holotoxin binding to the cell and has been described as a mucosal adjuvant for vaccines. In this work, the ctxB gene was genetically fused to the psaA gene from Streptococcus pneumoniae, a surface protein involved in its colonization in the host that is also considered a vaccine antigen candidate against this pathogen. The CTB-PsaA fusion protein was expressed in Escherichia coli, and the purified protein was used for intranasal immunization experiments in Balb/C mice. CTB-PsaA was able to induce both systemic and mucosal antibodies evaluated in serum, saliva, and in nasal and bronchial wash samples, showing that CTB-PsaA is a promising molecule to be investigated as S. pneumoniae vaccine antigen candidate.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
authors.forename: Ana Paula Mattos
authors.initials: AP
authors.lastname: Arêas
authors.forename: Maria Leonor Sarno
authors.initials: ML
authors.lastname: Oliveira
authors.forename: Eliane Namie
authors.initials: EN
authors.lastname: Miyaji
authors.forename: Luciana Cezar Cerqueira
authors.initials: LC
authors.lastname: Leite
authors.forename: Karina Araújo
authors.initials: KA
authors.lastname: Aires
authors.forename: Waldely Oliveira
authors.initials: WO
authors.lastname: Dias
authors.forename: Paulo Lee
authors.initials: PL
authors.lastname: Ho
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000942:Antigens, Bacterial / Q000235:genetics / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology*; D002772:Cholera Toxin / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; C051592:pneumococcal surface protein A; D002772:Cholera Toxin