[Effect of recombinant adenoviruses with CD/TK fusion suicide gene on human hepatocellular carcinoma cells].
OBJECTIVE
To investigate gene-therapy for human hepatocellular carcinoma with adenovirus vectors by double suicide gene CD/TK.


METHODS
Double suicide gene CD/TK was liberated from eukaryotic vectors pCEA-CD/TK and subcloned into shuttle vectors, and the transfer plasmid pAdtrack-CMV-CD/TK was formed after linearizing with Pac 1. It was recombinated with pAdeasy-1 in bacteria BJ5183. The identified adenovirus plasmid was digested by Pac1 and was transfected into 293 cells to pack the adenoviruses. After PCR determination, its titre was measured, and the infection rate and efficacy were tested in human hepatocellular carcinoma cells.


RESULTS
pAdtrack-CMV-CD/TK and pAd-CD/TK were tested by endonuclease digestion. Ad-CD/TK was produced in 293 cells, and the human hepatocellular carcinoma cells (SMMC7721) infected by Ad-CD/TK were killed after 5-FC was used, and bystander effects were observed.


CONCLUSIONS
Recombinant adenoviruses with CD/TK fusion suicide gene have a high infection rate and efficacy for human hepatocellular carcinoma cells.
Wang
Chang-Jun
CJ
Department of Oncology, Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510130, China. wangchj66@hotmail.com
Li
Jian-Jun
JJ
Wen
Xiao-Min
XM
Deng
Li
L
D000256:Adenoviridae / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000628:therapy*; D043525:Cytosine Deaminase / Q000235:genetics*; D015316:Genetic Therapy; D006801:Humans; D008113:Liver Neoplasms / Q000628:therapy*; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics*
D013937:Thymidine Kinase; D043525:Cytosine Deaminase