title: The interaction of Pseudomonas aeruginosa PAK with human and animal respiratory tract cell lines.
abstract: A major virulence factor of a common human pathogen Pseudomonas aeruginosa, was investigated to determine if it dominated attachment interactions in a variety of in vitro cell culture systems. It was found that Type-IV pilus-type mechanisms, which mediated the attachment of P. aeruginosa to three human respiratory tract cell lines (A549, BEAS-2B and RPMI 2650) also mediated attachment to two respiratory tract cell lines from mouse (C57) and rat (L-2) to a similar degree. Significant differences were found in the number of P. aeruginosa associated with the human, rat and mouse cell lines. Additionally, differences were also found between A547, C57 and L-2 cells with respect to the moieties that P. aeruginosa interacted with at the level of the cell surface, suggesting that asialo-GM1 ligands were not the only structure that this bacterium could interact with in order to associate with host cells.
authors.affiliation: Department of Biomedical Sciences, Dstl Porton Down, Salisbury, Wilts SP4 0JQ, UK.
authors.forename: Joy
authors.initials: J
authors.lastname: Hambrook
authors.forename: Richard
authors.initials: R
authors.lastname: Titball
authors.forename: Christopher
authors.initials: C
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D015169:Colony Count, Microbial; D004847:Epithelial Cells / Q000382:microbiology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D005677:G(M1) Ganglioside / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D051381:Rats; D020545:Respiratory Mucosa / Q000166:cytology / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D036781:Fimbriae Proteins; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside