The knockout mouse project.
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.
Austin
Christopher P
CP
National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA. austinc@mail.nih.gov
Battey
James F
JF
Bradley
Allan
A
Bucan
Maja
M
Capecchi
Mario
M
Collins
Francis S
FS
Dove
William F
WF
Duyk
Geoffrey
G
Dymecki
Susan
S
Eppig
Janan T
JT
Grieder
Franziska B
FB
Heintz
Nathaniel
N
Hicks
Geoff
G
Insel
Thomas R
TR
Joyner
Alexandra
A
Koller
Beverly H
BH
Lloyd
K C Kent
KC
Magnuson
Terry
T
Moore
Mark W
MW
Nagy
Andras
A
Pollock
Jonathan D
JD
Roses
Allen D
AD
Sands
Arthur T
AT
Seed
Brian
B
Skarnes
William C
WC
Snoddy
Jay
J
Soriano
Philippe
P
Stewart
David J
DJ
Stewart
Francis
F
Stillman
Bruce
B
Varmus
Harold
H
Varticovski
Lyuba
L
Verma
Inder M
IM
Vogt
Thomas F
TF
von Melchner
Harald
H
Witkowski
Jan
J
Woychik
Richard P
RP
Wurst
Wolfgang
W
Yancopoulos
George D
GD
Young
Stephen G
SG
Zambrowicz
Brian
B
D000483:Alleles; D000818:Animals; D036281:Genetic Research; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D035845:Research Embryo Creation / Q000191:economics