Optimal timing to repopulation of resident alveolar macrophages with donor cells following total body irradiation and bone marrow transplantation in mice.
To determine the time required to repopulate mouse lungs with donor alveolar macrophages following total body irradiation (TBI) and bone marrow transplantation (BMT), C57Bl/6 mice were subjected to TBI with 900 cGy, followed by transplantation of bone marrow cells from mice expressing green fluorescent protein (GFP) in their somatic cells. The mice were euthanized at either 30 (n=5), 60 (n=5) or 90 (n=5) days following BMT. Thirty days following transplantation, 87.8 +/- 3.9% (mean +/- S.E.M.) circulating leukocytes in recipient mice were derived from the donor, as determined by fluorescence activated cell sorting (FACS) analysis for GFP. However, only 46.9 +/- 7.4% of the resident alveolar cells expressed GFP, indicating incomplete repopulation. By day 60 post-transplantation, the percentage of bronchoalveolar lavage fluid (BALF) cells expressing GFP reached 74.5 +/- 2.4%, remaining stable 90 days after transplantation (80.4 +/- 1.9%). We conclude that 60 days after TBI with 900 cGy and bone marrow transplantation, the majority of the lung resident alveolar macrophages is of donor origin. This study provides useful information regarding the time of reconstitution with donor alveolar macrophages in the pulmonary airspaces of recipient mice following marrow transplantation.
Matute-Bello
Gustavo
G
Pulmonary and Critical Care Division, Department of Medicine, University of Washington, Seattle, WA 98108, USA.
Lee
Janet S
JS
Frevert
Charles W
CW
Liles
W Conrad
WC
Sutlief
Steven
S
Ballman
Kimberly
K
Wong
Venus
V
Selk
Amy
A
Martin
Thomas R
TR
D019264:Adoptive Transfer; D000818:Animals; D016026:Bone Marrow Transplantation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014916:Whole-Body Irradiation
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins