title: Reversible mechanical unfolding of single ubiquitin molecules.
abstract: Single-molecule manipulation techniques have enabled the characterization of the unfolding and refolding process of individual protein molecules, using mechanical forces to initiate the unfolding transition. Experimental and computational results following this approach have shed new light on the mechanisms of the mechanical functions of proteins involved in several cellular processes, as well as revealed new information on the protein folding/unfolding free-energy landscapes. To investigate how protein molecules of different folds respond to a stretching force, and to elucidate the effects of solution conditions on the mechanical stability of a protein, we synthesized polymers of the protein ubiquitin and characterized the force-induced unfolding and refolding of individual ubiquitin molecules using an atomic-force-microscope-based single-molecule manipulation technique. The ubiquitin molecule was highly resistant to a stretching force, and the mechanical unfolding process was reversible. A model calculation based on the hydrogen-bonding pattern in the native structure was performed to explain the origin of this high mechanical stability. Furthermore, pH effects were studied and it was found that the forces required to unfold the protein remained constant within a pH range around the neutral value, and forces decreased as the solution pH was lowered to more acidic values.
authors.affiliation: Department of Chemistry, National Dong Hwa University, Hualien, Taiwan.
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Chyan
authors.forename: Fan-Chi
authors.initials: FC
authors.lastname: Lin
authors.forename: Haibo
authors.initials: H
authors.lastname: Peng
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Yuan
authors.forename: Chung-Hung
authors.initials: CH
authors.lastname: Chang
authors.forename: Sheng-Hsien
authors.initials: SH
authors.lastname: Lin
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D003198:Computer Simulation; D004548:Elasticity; D019563:Mechanics; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013314:Stress, Mechanical; D013329:Structure-Activity Relationship; D025801:Ubiquitin / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D025801:Ubiquitin