Adipogenic and antiapoptotic protein levels in human adipose stromal cells after weight loss.
OBJECTIVE
Obesity is a major risk factor for type 2 diabetes and cardiovascular disease. However, current strategies to achieve sustained weight loss are often unsuccessful. Fat reaccumulation might be favored by enhanced adipose cell differentiation or survival in the postreduced state.


METHODS
We measured adipogenic and apoptotic protein expression in subcutaneous abdominal adipose stromal-vascular cells from 10 obese patients (7 women and 3 men) that were obtained before and after a 16% weight loss in a medically supervised weight loss program.


RESULTS
After weight loss, protein expression was 2.4-fold higher (p < 0.005) for p42 C/CAAT enhancer binding protein alpha, but there was no change for peroxisome proliferator-activated receptor gamma1; both of these are adipogenic regulators. For neuronal apoptosis inhibitory protein, a protein associated with adipose cell apoptotic resistance, there was a rise of 1.7-fold (p < 0.02).


CONCLUSIONS
Alterations in C/CAAT enhancer binding protein alpha and neuronal apoptosis inhibitory protein expression occurred in human adipose stromal-vascular cells after weight loss in a pilot study of 10 patients. It will be important for future studies to directly examine whether the adipogenic and antiapoptotic capacity of these cells is changed after weight loss.
Aubin
Denise
D
Ottawa Health Research Institute, Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario K1Y 4E9, Canada.
Gagnon
AnneMarie
A
Grunder
Laura
L
Dent
Robert
R
Allen
Murray
M
Sorisky
Alexander
A
D000273:Adipose Tissue / Q000098:blood supply / Q000737:chemistry*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001824:Body Constitution; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051616:Neuronal Apoptosis-Inhibitory Protein; D009474:Neurons / Q000737:chemistry; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis; D017154:Stromal Cells / Q000737:chemistry; D014157:Transcription Factors / Q000032:analysis; D015431:Weight Loss / Q000502:physiology*
D022763:CCAAT-Enhancer-Binding Protein-alpha; C496800:NAIP protein, human; D009419:Nerve Tissue Proteins; D051616:Neuronal Apoptosis-Inhibitory Protein; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors