title: Extensive genomic polymorphism within Mycobacterium avium.
abstract: We have initiated comparative genomic analysis of Mycobacterium avium subspecies by DNA microarray, uncovering 14 large sequence polymorphisms (LSPs) comprising over 700 kb that distinguish M. avium subsp. avium from M. avium subsp. paratuberculosis. Genes predicted to encode metabolic pathways were overrepresented in the LSPs, and analysis revealed a polymorphism within the mycobactin biosynthesis operon that potentially explains the in vitro mycobactin dependence of M. avium subsp. paratuberculosis.
authors.affiliation: McGill University Health Center, Montreal, Quebec, Canada.
authors.forename: Makeda
authors.initials: M
authors.lastname: Semret
authors.forename: Gary
authors.initials: G
authors.lastname: Zhai
authors.forename: Serge
authors.initials: S
authors.lastname: Mostowy
authors.forename: Cynthia
authors.initials: C
authors.lastname: Cleto
authors.forename: David
authors.initials: D
authors.lastname: Alexander
authors.forename: Gerard
authors.initials: G
authors.lastname: Cangelosi
authors.forename: Debby
authors.initials: D
authors.lastname: Cousins
authors.forename: Desmond M
authors.initials: DM
authors.lastname: Collins
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Behr
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D009162:Mycobacterium avium / Q000235:genetics* / Q000378:metabolism; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010080:Oxazoles / Q000378:metabolism; D011110:Polymorphism, Genetic
chemical_list: D004269:DNA, Bacterial; D010080:Oxazoles; C018608:mycobactins