Ectopic expression of a cold-inducible transcription factor, CBF1/DREB1b, in transgenic rice (Oryza sativa L.).
The gene encoding C-repeat/dehydration-responsive element binding factor 1 (CBF1/DREB1b) of Arabidopsis was introduced into rice (Oryza sativa L.) under the control of the maize ubiquitin promoter. Its incorporation and expression in transgenic rice plants were confirmed by DNA and RNA gel-blot analyses. Cold tolerance in the transgenics was not significantly different from that of the wild-type plants, as determined by ion leakage, chlorophyll fluorescence, and survival rates. However, the cold-responsive genes lip5, lip9, and OsDhn1 were up-regulated in the transgenic plants, suggesting that the cold signal transduction pathway involving CBF1 is partially conserved in this cold-labile plant.
Lee
Sang-Choon
SC
Department of Life Science, Sogang University, Seoul 121-742, Korea.
Huh
Kyung-Won
KW
An
Kyungsook
K
An
Gynheung
G
Kim
Seong-Ryong
SR
D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D036226:Seedlings / Q000033:anatomy & histology; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors; D002734:Chlorophyll