Ameroid rings for gradual chronic constriction of the sciatic nerve in rats: contribution of different nerves to neuropathic pain.
Mononeuropathy was induced by placing an ameroid ring around the sciatic nerve and was compared with chronic constriction injury (CCI) of the sciatic nerve [Pain 33 (1988) 87] in rats. Mechanical allodynia was assessed and the role of sciatic and saphenous afferents (Adelta and C) in thermal hyperalgesia investigated. A shorter duration of mechanical allodynia in ameroid rats as compared to CCI rats was observed. Thermal hyperalgesia was observed in the saphenous innervated skin of the hindpaw for Adelta and C nociceptors in ameroid and for Adelta nociceptors only in CCI rats, respectively. The sciatic innervated skin showed a thermal hypoalgesia with a fast onset for Adelta afferents and a slower onset for C afferents in CCI and ameroid rats. The duration of both thermal hypo- and hyperalgesia was longer in ameroid rats. We conclude that ameroid rings are a useful tool for the investigation of long-duration hyperalgesic effects of nerve injury, as the effects were more stable and seen for a longer time (>8 weeks) as compared to the CCI model. The uninjured saphenous afferents, in particular C fibers, mediate thermal hyperalgesia after chronic constriction of the sciatic nerve using an ameroid ring.
Tzabazis
A
A
Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, S-276 Stanford, CA 94305, USA.
Kim
P H
PH
Sweitzer
S M
SM
Yeomans
D C
DC
D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D002364:Caseins; D003251:Constriction, Pathologic; D020100:Hydrogels / Q000009:adverse effects*; D006930:Hyperalgesia / Q000503:physiopathology; D008297:Male; D020422:Mononeuropathies / Q000150:complications*; D009437:Neuralgia / Q000209:etiology*; D010147:Pain Measurement; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D015203:Reproducibility of Results; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology*; D012867:Skin / Q000294:innervation / Q000503:physiopathology; D013997:Time Factors
D001672:Biocompatible Materials; D002364:Caseins; D020100:Hydrogels; C009719:ameroid