Identification of a promoter motif regulating the major DNA damage response mechanism of Mycobacterium tuberculosis.
The principal response of many bacteria to DNA damage is mediated by a mechanism dependent on the LexA and RecA proteins. However, Mycobacterium tuberculosis was recently reported to regulate a majority of DNA repair genes independently of RecA and LexA, suggesting that an unknown RecA/LexA-independent mechanism controls the major DNA damage response pathway in this organism. Here we have identified a motif tTGTCRgtg-8nt-TAnnnT that defines a novel RecA/LexA-independent promoter (RecA-NDp) of M. tuberculosis. Furthermore, we show that the RecA-NDp type of promoter precedes DNA repair genes in other Actinomycetales.
Gamulin
Vera
V
Department of Molecular Biology, Rudjer Boskovic Institute, pp180, 10002 Zagreb, Croatia.
Cetkovic
Helena
H
Ahel
Ivan
I
D000192:Actinomycetales / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D016384:Consensus Sequence; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D018085:Regulon; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D012808:Sigma Factor / Q000502:physiology; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D012808:Sigma Factor; D000099160:LexA Repressor Protein