Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene.
Limited but significant sequence similarity has been observed between an uncharacterized human protein, SIN1, and the S. pombe SIN1, Dictyostelium RIP3 and S. cerevisiae AVO1 proteins. The human Sin1 gene has been automatically predicted (MAPKAP1; GenBank accession number ); however, this sequence appears to be incomplete. In this study, we have cloned and characterized the full-length human Sin1 mRNA and identified a highly conserved domain that defines the family of SIN1 orthologues, members of which are widely distributed in the fungal and metazoan kingdoms. We demonstrate that Sin1 transcripts can use alternative polyadenylation signals and describe a number of Sin1 splice variants that potentially encode functionally different isoforms.
Schroder
Wayne
W
The Queensland Institute of Medical Research, 300 Herston Road, Brisbane, 4029, Australia. wayneS@qimr.edu.au
Cloonan
Nicole
N
Bushell
Gillian
G
Sculley
Tom
T
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics* / Q000378:metabolism; D026723:Polyadenylation; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018076:DNA, Complementary; C493277:MAPKAP1 protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D011061:Poly A