Evidence for the presence of ferritin in plant mitochondria.
In this work, evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems and Arabidopsis thaliana cell cultures. The proteins were separated by SDS/PAGE. A protein, with an apparent molecular mass of approximately 25-26 kDa (corresponding to that of ferritin), was cross-reacted with an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunoprecipitation. The purified protein was analyzed by MALDI-TOF mass spectrometry and the results of both mass finger print and peptide fragmentation by post source decay assign the polypeptide sequence to the pea ferritin (P < 0.05). The mitochondrial localization of ferritin was also confirmed by immunocytochemistry experiments on isolated mitochondria and cross-sections of pea stem cells. The possible role of ferritin in oxidative stress of plant mitochondria is discussed.
Zancani
Marco
M
Dipartimento di Biologia ed Economia Agro-Industriale, Sezione di Biologia Vegetale, Università di Udine, Italy.
Peresson
Carlo
C
Biroccio
Antonino
A
Federici
Giorgio
G
Urbani
Andrea
A
Murgia
Irene
I
Soave
Carlo
C
Micali
Fulvio
F
Vianello
Angelo
A
Macrì
Francesco
F
D017360:Arabidopsis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000737:chemistry*; D008970:Molecular Weight; D018532:Pisum sativum / Q000737:chemistry; D059828:Plant Cells; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D011233:Precipitin Tests; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins; D005293:Ferritins