title: Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development.
abstract: The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3 protein containing novel repeats and zinc fingers described as protein interaction domains. VAR3 interacts specifically in yeast and in vitro with NCED4, a putative polyene chain or carotenoid dioxygenase, and both VAR3 and NCED4 accumulate in the chloroplast stroma. Metabolic profiling demonstrates that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development.
authors.affiliation: Institute of Molecular Biology, Copenhagen University, Øster Farimagsgade 2A, 1353 Copenhagen K, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Naested
authors.forename: Agnethe
authors.initials: A
authors.lastname: Holm
authors.forename: Tom
authors.initials: T
authors.lastname: Jenkins
authors.forename: H Bjørn
authors.initials: HB
authors.lastname: Nielsen
authors.forename: Cassandra A
authors.initials: CA
authors.lastname: Harris
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.forename: Mathias
authors.initials: M
authors.lastname: Andersen
authors.forename: Alexandra
authors.initials: A
authors.lastname: Mant
authors.forename: Henrik
authors.initials: H
authors.lastname: Scheller
authors.forename: Bilal
authors.initials: B
authors.lastname: Camara
authors.forename: Ole
authors.initials: O
authors.lastname: Mattsson
authors.forename: John
authors.initials: J
authors.lastname: Mundy
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D047908:Intracellular Signaling Peptides and Proteins; D008027:Light; D008969:Molecular Sequence Data; D010641:Phenotype; D010858:Pigmentation; D010860:Pigments, Biological / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010860:Pigments, Biological; D011993:Recombinant Fusion Proteins; C504622:VAR3 protein, Arabidopsis