Cytokinesis monitoring during development; rapid pole-to-pole shuttling of a signaling protein by localized kinase and phosphatase in Caulobacter.
For successful generation of different cell types by asymmetric cell division, cell differentiation should be initiated only after completion of division. Here, we describe a control mechanism by which Caulobacter couples the initiation of a developmental program to the completion of cytokinesis. Genetic evidence indicates that localization of the signaling protein DivK at the flagellated pole prevents premature initiation of development. Photobleaching and FRET experiments show that polar localization of DivK is dynamic with rapid pole-to-pole shuttling of diffusible DivK generated by the localized activities of PleC phosphatase and DivJ kinase at opposite poles. This shuttling is interrupted upon completion of cytokinesis by the segregation of PleC and DivJ to different daughter cells, resulting in disruption of DivK localization at the flagellated pole and subsequent initiation of development in the flagellated progeny. Thus, dynamic polar localization of a diffusible protein provides a control mechanism that monitors cytokinesis to regulate development.
Matroule
Jean-Yves
JY
Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520, USA.
Lam
Hubert
H
Burnette
Dylan T
DT
Jacobs-Wagner
Christine
C
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016934:Caulobacter / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000378:metabolism; D000071677:Histidine Kinase; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics
D001426:Bacterial Proteins; C095478:DivK protein, Caulobacter crescentus; D011494:Protein Kinases; D000071677:Histidine Kinase; D010749:Phosphoprotein Phosphatases