title: Visualization of plasma membrane compartmentalization with patterned lipid bilayers.
abstract: Micrometer-size patterned lipid bilayers containing liganded lipids are used to control the location and size of receptor clusters and enable direct visualization of structural reorganization of cellular components. Subsequent to concentration of Fcepsilon receptor I, the mast cell receptor for IgE, and colocalized tyrosine phosphorylation activity, Lyn kinase and other proteins anchored to the inner leaflet of the plasma membrane redistribute selectively with the receptor clusters in a process that depends on actin polymerization. Surprisingly, outer leaflet components characteristically associated with lipid rafts do not detectably coredistribute with these inner leaflet components. Cell activation using patterned surfaces provides unique insights into cell membrane structural organization, revealing dynamic, large-scale uncoupling of inner and outer leaflet components of lipid rafts.
authors.affiliation: Department of Chemistry and Chemical Biology and School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: David
authors.initials: D
authors.lastname: Holowka
authors.forename: Harold G
authors.initials: HG
authors.lastname: Craighead
authors.forename: Barbara
authors.initials: B
authors.lastname: Baird
mesh_terms: D000199:Actins / Q000737:chemistry; D000818:Animals; D045744:Cell Line, Tumor; D021961:Cell Membrane Structures / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure; D017930:Genes, Reporter; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D018613:Microscopy, Confocal; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats; D017455:Receptors, IgE / Q000032:analysis; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D000199:Actins; D008051:Lipid Bilayers; D008563:Membrane Lipids; D017455:Receptors, IgE; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases