[The morphology observation, mechanics intensity test and genetic analysis of brittle culm mutant bcm581-1 in rice].
A rice brittle culm mutant bcm581-1 which derived from the Ds transposone transformation population was found, but the mutant was identified that it was not to be induced by Ds transposone insertion through PCR. The examination of the vascular bundle and cortical fibre cells in culm under the light and electron microscope showed that, the number of cortical vascular bundle of mutant was much more, the hollow among the cortical vascular bundle was deeper, and the cell walls of cortical fibre cells were thinner than the normal. The test of culm mechanics intensity showed that the load, elongation, strain, and stress of bcm581-1 were 5-9 times lower than normal. The moisture content and the wide fibre content of culm were test, the former was 3.5% higher, but the latter was 8.12% lower than normal. The analysis of genetic segregation in F2 and F1B1 population indicated that the brittle culm mutant was controlled by one recessive gene.
Shen
Ge Zhi
GZ
Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106.
Wang
Xin Qi
XQ
Wang
Jiang
J
Wan
Xin Shan
XS
Li
Lin
L
Zhang
Jing Liu
JL
D001696:Biomechanical Phenomena; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D018547:Plant Stems / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure