A new C-type animal lectin isolated from Bothrops pirajai is responsible for the snake venom major effects in the isolated kidney.
We investigated the biochemical and biological effects of a new C-type galactoside specific lectin termed BPL that was isolated from the snake venom of Bothrops pirajai. This lectin was purified using size exclusion HPLC followed by an immobilized lactose affinity column. The purified BPL was homogeneous by reverse phase HPLC and SDS-PAGE. We evaluated the nephrotoxicity of the whole venom of B. pirajai and its lectin. The whole venom of B. pirajai (10 microg/mL) showed similar results as those observed for BPL (3, 10 and 30 microg/mL) evaluated by the perfused rat kidney method. They caused reductions in perfusion pressure (Control120 = 110.28 +/- 3.69; BP120 = 70.70 +/- 2.40*; BPL3(120) = 113.20 +/- 4.40; BPL10(120) = 67.80 +/- 3.00*; BPL30(120) = 64.90 +/- 3.50* mmHg; *: P < 0.05), renal vascular resistance, urinary flow, glomerular filtration rate (Control90 = 0.695 +/- 0.074; BP90 = 0.142 +/- 0.032*; BPL3(90) = 0.314 +/- 0.064; BPL10(90) = 0.250 +/- 0.038*; BPL30(90) = 0.088 +/- 0.021* mLg(-1) min(-1); *: P < 0.05) and sodium (Control120 = 81.28 +/- 0.26; BP120 = 55.71 +/- 5.72*; BPL3(120) = 80.94 +/- 0.93; BPL10(120) = 65.23 +/- 1.47*; BPL30(120) = 76.03 +/- 1.70* %; *: P < 0.05), potassium and chloride tubular transport. Neither whole venom nor purified BPL induced direct vasoactive effects in perfused arteriolar mesenteric bed, and BPL did not potentiate bradykinin contraction in the ileum. We postulate that both B. pirajai and BPL promoted the same renal effects probably caused by the release of inflammatory mediators.
Havt
Alexandre
A
Departament of Physiology and Pharmacology, Federal University of Ceará, Coronel Nunes de Melo1127, CEP 60430-270 Fortaleza, Ceará, Brazil. ahavt@secrel.com.br
Toyama
Marcos Hikari
MH
do Nascimento
Nilberto Robson Falcão
NR
Toyama
Daniela Oliveira
DO
Nobre
Arlandia Cristina Lima
AC
Martins
Alice Maria Costa
AM
Barbosa
Paulo Sérgio Ferreira
PS
Novello
Jose Camillo
JC
Boschero
Antonio C
AC
Carneiro
Everardo M
EM
Fonteles
Manassés Claudino
MC
Monteiro
Helena Serra Azul
HS
D000818:Animals; D017837:Bothrops; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry* / Q000633:toxicity*; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology*; D037181:Lectins, C-Type / Q000302:isolation & purification; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014655:Vascular Resistance / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects
D003435:Crotalid Venoms; D018836:Inflammation Mediators; D037181:Lectins, C-Type