title: Methodological standardization for a multi-institutional in vivo trial of localized 31P MR spectroscopy in human cancer research. In vitro and normal volunteer studies.
abstract: A multi-institutional group has been created to demonstrate the utility of in vivo 31P magnetic resonance spectroscopy (31P-MRS) to study human cancers in vivo. This review is concerned with the novel problems concerning quality control in this large multinational trial of 31P MRS. Our results show that the careful and systematic performance of the quality control tests depicted here (standardized dual 1H/31P tuned radiofrequency probe, quality control procedures, routine use of 1H irradiation while acquiring 31P MR signals) has ensured comparable results between the different institutions. In studies made in vitro, the root-mean-square error was 3.6 %, and in muscle of healthy volunteers in vivo the coefficients of variance for the ratios phosphocreatine/nucleotide-triphosphates, phosphocreatine/noise and nucleotide-triphosphate/noise were 12.2, 7.0 and 10.8 %, respectively. The standardization of the acquisition protocol for in vivo-localized 31P MR spectroscopy across the different institutions has resulted in comparable in vivo data, decreasing the possible problems related to a research study carried out under a multi-institutional setting.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, PA, USA. fa2003@columbia.edu
authors.forename: F
authors.initials: F
authors.lastname: Arias-Mendoza
authors.forename: K
authors.initials: K
authors.lastname: Zakian
authors.forename: A
authors.initials: A
authors.lastname: Schwartz
authors.forename: F A
authors.initials: FA
authors.lastname: Howe
authors.forename: J A
authors.initials: JA
authors.lastname: Koutcher
authors.forename: M O
authors.initials: MO
authors.lastname: Leach
authors.forename: J R
authors.initials: JR
authors.lastname: Griffiths
authors.forename: A
authors.initials: A
authors.lastname: Heerschap
authors.forename: J D
authors.initials: JD
authors.lastname: Glickson
authors.forename: S J
authors.initials: SJ
authors.lastname: Nelson
authors.forename: J L
authors.initials: JL
authors.lastname: Evelhoch
authors.forename: H C
authors.initials: HC
authors.lastname: Charles
authors.forename: T R
authors.initials: TR
authors.lastname: Brown
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D004983:Ethanolamines / Q000032:analysis; D006801:Humans; D038622:Internationality; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards*; D015337:Multicenter Studies as Topic / Q000379:methods* / Q000592:standards*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D010759:Phosphorus Isotopes; D010767:Phosphorylcholine / Q000032:analysis; D011785:Quality Assurance, Health Care / Q000379:methods / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D012106:Research / Q000592:standards; D012107:Research Design; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D004983:Ethanolamines; D010759:Phosphorus Isotopes; D010767:Phosphorylcholine; C005448:phosphorylethanolamine