[Experimental study of Ginkgo biloba exocarp polysaccharides on HL-60 cells in vitro].
OBJECTIVE
To study the effect of Ginkgo biloba exocarp polysaccharides (GBEP) on HL-60 cells in vitro.


METHODS
Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of HL-60 cells.


RESULTS
GBEP (40-160 mg/L, 48 h) could inhibit HL-60 cells proliferation and the expression of proliferation-promotion gene c-myc, induce HL-60 cells apoptosis and down-regulate the expression of apoptosis-inhibitory gene bcl-2.


CONCLUSIONS
Influence of GBEP on HL-60 cells proliferation and apoptosis may relate to its effects on the expression of proliferation-promotion gene c-myc and apoptosis-inhibitory gene bcl-2.
Xu
Aihua
A
Medical College of Yangzhou University, Yangzhou 225001.
Chen
Huasheng
H
Sun
Buchan
B
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D020441:Ginkgo biloba / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D024301:Plant Bark / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D012639:Seeds / Q000737:chemistry; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc