Gastroprotective effect of the mixture of alpha- and beta-amyrin from Protium heptaphyllum: role of capsaicin-sensitive primary afferent neurons.
This investigation evaluated the role of capsaicin-sensitive afferent neurons in the gastroprotective effect of alpha- and beta-amyrin, a triterpenoid mixture isolated from Protium heptaphyllum resin. Gastric mucosai damage was induced in mice by intragastric ethanol and assessed by planimetry. Mice pretreated orally with the amyrin mixture (50 and 100 mg/kg) or capsaicin (2.5 and 5 mg/kg), the pungent principle from red hot peppers, showed a significantly lower intensity of ethanol-associated gastric mucosal damage, in relation to vehicle-treated controls. At higher doses both these agents produced either a diminished protection or no significant effect. The maximal gastroprotection that was observed at the dose of 100 mg/kg amyrin mixture was almost abolished in mice with their sensory afferents chemically ablated by a neurotoxic dose of capsaicin, suggesting that the gastro-protective mechanism of alpha- and beta-amyrin mixture involves at least in part the activation of capsaicin-sensitive primary afferent neurons.
Oliveira
Francisco A
FA
Vieira-Júnior
Gerardo M
GM
Chaves
Mariana H
MH
Almeida
Fernanda R C
FR
Santos
Kelcyana A
KA
Martins
Fabrício S
FS
Silva
Regilane M
RM
Santos
Flavia A
FA
Rao
Vietla S N
VS
D000284:Administration, Oral; D000818:Animals; D029403:Burseraceae; D002211:Capsaicin; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D000431:Ethanol; D005756:Gastritis / Q000139:chemically induced / Q000188:drug therapy*; D005765:Gastrointestinal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D009475:Neurons, Afferent / Q000187:drug effects; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D014934:Wood
D005765:Gastrointestinal Agents; D000431:Ethanol; D009828:Oleanolic Acid; C036380:beta-amyrin; D002211:Capsaicin