Patterning and separating infected bacteria using host-parasite and virus-antibody interactions.
Bacteria were selectively deposited on substrates patterned with poly(ethylene glycol) (PEG) microstructures by using host-parasite and virus-antibody interactions. In this scheme viruses were used to attach onto a host bacterium, Escherichia coli (E. coli). The E. coli expressing the virus were selectively adhered to the regions pretreated with an antibody against the virus proteins while E. coli without the virus showed no selectivity. Single or aggregated cell arrays were fabricated depending on the initial pattern size with respect to the size of E. coli. The current approach could be a general route to spatially positioning or controlling adhesion of other biological species that are not accessible by conventional methods and as a tool for separating and isolating specific cell populations based on host-parasite interactions.
Suh
Kahp Y
KY
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Korea.
Khademhosseini
Ali
A
Yoo
Pil J
PJ
Langer
Robert
R
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001422:Bacterial Adhesion / Q000502:physiology; D017104:Bacteriophage M13 / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004926:Escherichia coli / Q000166:cytology* / Q000302:isolation & purification / Q000502:physiology / Q000821:virology*; D006790:Host-Parasite Interactions / Q000502:physiology; D008422:Materials Testing; D010781:Photography / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D020099:Coated Materials, Biocompatible; D011092:Polyethylene Glycols