[The anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SMMC-7721 cells].
OBJECTIVE
To explore the anti-cancer effects of siRNAs targeting hTERT in SMMC-7721 cells.


METHODS
Two siRNAs targeting hTERT mRNA were designed and synthesized by T7 transcription system in vitro. MMT, RT-PCR and Western blot were applied to evaluate effects on inhibiting cell growth, hTERT mRNA and protein expression in SMMC-7721 cells.


RESULTS
siRNAs decreased cell proliferation in a dose-dependent manner. At a concentration of 100 nmol/L, siRNAs exhibited obvious effects on inhibiting hTERT mRNA and protein expression in SMMC-7721 cells.


CONCLUSIONS
siRNAs targeting hTERT have significant inhibitory effects on hTERT gene expression in SMMC-7721 cells. siRNA has the possibility to become a new anti-cancer agent
Zheng
Su-Jun
SJ
Institute for Viral Hepatitis and the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China.
Xia
Yun
Y
Ren
Hong
H
Zhong
Sen
S
Yang
Ying
Y
Tao
Peng
P
Wang
Sheng-Qi
SQ
D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D018390:Gene Targeting; D015316:Genetic Therapy; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000235:genetics*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics*
D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D019098:Telomerase