Desulfatibacillum alkenivorans sp. nov., a novel n-alkene-degrading, sulfate-reducing bacterium, and emended description of the genus Desulfatibacillum.
An alkene-degrading, sulfate-reducing bacterium, strain PF2803T, was isolated from oil-polluted sediments (Fos Harbour, France). The cells were found to be Gram-negative, non-sporulating, non-motile and to have a slightly curved rod shape. Optimum growth occurred at 1 % (w/v) NaCl, pH 6.8 and 28-30 degrees C. Strain PF2803T oxidized alkenes (from C8 to C23). The G + C content of the genomic DNA was 57.8 mol% (HPLC). On the basis of 16S rRNA gene sequence analyses, strain PF2803T belongs to the family 'Desulfobacteraceae' in the class 'Deltaproteobacteria', with Desulfatibacillum aliphaticivorans as its closest relative (99.6 % identity). Comparative sequence analyses of the dissimilatory sulfite reductase (dsrAB) gene supported the affiliation of strain PF2803T to the genus Desulfatibacillum. DNA-DNA hybridization with its closest taxon demonstrated 48.4 % similarity. On the basis of the results of physiological and genetic analyses, strain PF2803T is identified as a novel species of the genus Desulfatibacillum, for which the name Desulfatibacillum alkenivorans sp. nov. is proposed. The type strain is PF2803T (= DSM 16219T = ATCC BAA-924T).
Cravo-Laureau
Cristiana
C
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
Matheron
Robert
R
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
Joulian
Catherine
C
Laboratoire de Microbiologie IRD, UR 101, IFR-BAIM case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Cayol
Jean-Luc
JL
Laboratoire de Microbiologie IRD, UR 101, IFR-BAIM case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Hirschler-Réa
Agnès
A
Laboratoire de Microbiologie, IMEP CNRS UMR 6116, Faculté des Sciences et Techniques de Saint-Jérôme, case 452, 13397 Marseille cedex 20, France.
D000475:Alkenes / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020564:Deltaproteobacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D004783:Environmental Microbiology; D005602:France; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013171:Spores, Bacterial; D013431:Sulfates / Q000378:metabolism*
D000475:Alkenes; D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates; D012965:Sodium Chloride