Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae.
OSH1 belongs to a seven-member gene family in yeast that is related to mammalian oxysterol-binding protein (OSBP). Here, we investigate the targeting of Osh1p to nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae. NV junctions are interorganelle interfaces mediated by Nvj1p in the nuclear envelope and Vac8p on the vacuole membrane. Together, Nvj1p and Vac8p form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by a process termed piecemeal microautophagy of the nucleus (PMN). Osh1p is targeted to NV junctions proportional to NVJ1 expression through a physical association with Nvj1p. NV junctions per se are not required for this targeting because Osh1p colocalizes with Nvj1p in the absence of Vac8p. NV-junction-associated Osh1p is also a substrate for PMN degradation. Although OSH1 is not required for NV-junction formation or PMN, PMN is defective in cells lacking the yeast OSBP family (Osh1p to Osh7p). By contrast, the vesicular targeting of aminopeptidase I to the vacuole by macroautophagy is not dependent on the Osh protein family. We conclude the formation of nuclear PMN vesicles requires the overlapping activities of Osh1p and other Osh family members.
Kvam
Erik
E
Department of Biology, University of Rochester, 436 Hutchison Hall, NY 14627, USA.
Goldfarb
David S
DS
D000626:Aminopeptidases / Q000737:chemistry; D001343:Autophagy; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005838:Genotype; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D008074:Lipoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology*; D013997:Time Factors; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; C499847:Nvj1 protein, S cerevisiae; D018160:Receptors, Cytoplasmic and Nuclear; C089501:SWH1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C111373:VAC8 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins; D000626:Aminopeptidases