Borrelia turcica sp. nov., isolated from the hard tick Hyalomma aegyptium in Turkey.
Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour-Stoenner-Kelly (BSK) II medium; the tick samples were taken from the Istanbul area in northwestern Turkey [Güner et al. (2003). Microbiology 149, 2539-2544]. Here is presented a detailed characterization of the spirochaete. Electron microscopy revealed that strain IST7T is morphologically similar to other spirochaetes of the genus Borrelia and possesses 15 to 16 flagellae that emerge from both polar regions. PFGE analysis revealed the genome to comprise a linear chromosome of approximately 1 Mb; two large linear plasmids of approximately 145 and 140 kb, and several small plasmids ranging from 50 to 20 kb in size were also found. The 16S rRNA gene sequence of this Borrelia isolate exhibited 99.4 to 99.8 % identity with other strains isolated from H. aegyptium and less than 99 % similarity with those of other Borrelia species. A phylogenetic tree, generated from 16S rRNA gene sequences, demonstrated that the spirochaete isolates from H. aegyptium clustered together and branched off from both Lyme-disease-related and relapsing-fever-associated Borrelia species. A single copy of the rrs gene was detected in the genome of strain IST7T by Southern hybridization. DNA-DNA hybridization results showed that strain IST7T was distinct from Lyme-disease-related Borrelia, Borrelia burgdorferi and the relapsing-fever-associated species Borrelia hermsii. The G+C content of strain IST7T is 30.0 mol%. From these genetic features, a novel Borrelia species, Borrelia turcica sp. nov., is proposed; the type strain is IST7T (= JCM 11958T = DSM 16138T).
Güner
Ece S
ES
Medical School, Yeditepe University, Istanbul, Turkey.
Watanabe
Mutsumi
M
Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan.
Hashimoto
Naoya
N
Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan.
Kadosaka
Teruki
T
Department of Parasitology, Aichi Medical University, Aichi, 480-1195, Japan.
Kawamura
Yoshiaki
Y
Department of Microbiology, School of Medicine, Gifu University, Gifu, 500-8705, Japan.
Ezaki
Takayuki
T
Department of Microbiology, School of Medicine, Gifu University, Gifu, 500-8705, Japan.
Kawabata
Hiroki
H
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan.
Imai
Yasuyuki
Y
Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan.
Kaneda
Kazuhide
K
Department of Food Science, Shizuoka Eiwa Gakuen University Junior College, Shizuoka, 422-8005, Japan.|Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan.
Masuzawa
Toshiyuki
T
Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, 422-8526, Japan.
D000818:Animals; D001482:Base Composition; D026501:Blotting, Southwestern; D001898:Borrelia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005407:Flagella; D020459:Genes, rRNA; D026863:Ixodidae / Q000382:microbiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010957:Plasmids / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000662:veterinary; D014421:Turkey; D014426:Turtles / Q000469:parasitology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S