Exchange transfusion with entirely synthetic red-cell substitute albumin-heme into rats: physiological responses and blood biochemical tests.
Recombinant human serum albumin (rHSA) incorporating 2-[8-[N-(2-methylimidazolyl)]octanoyloxymethyl]-5,10,15,20-[tetrakis[alpha,alpha,alpha,alpha-o-(1-methylcyclohexanoyl)amino]phenyl]porphinatoiron(II) [albumin-heme (rHSA-heme)] is an artificial hemoprotein which has the capability to transport O(2) in vitro and in vivo. A 20% exchange transfusion with rHSA-heme into anesthetized rats has been performed to evaluate its clinical safety by monitoring the circulation parameters and blood parameters for 6 h after the infusion. Time course changes in all parameters essentially showed the same features as those of the control group (without infusion) and rHSA group (with administration of the same amount of rHSA). Blood biochemical tests of the withdrawn plasma at 6 h after the exchange transfusion have also been carried out. No significant difference was found between the rHSA-heme and rHSA groups, suggesting the initial clinical safety of this entirely synthetic O(2)-carrier as a red-cell substitute.
Huang
Yubin
Y
Advanced Research Institute for Science and Engineering, Waseda University,3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan.
Komatsu
Teruyuki
T
Yamamoto
Hisashi
H
Horinouchi
Hirohisa
H
Kobayashi
Koichi
K
Tsuchida
Eishun
E
D000418:Albumins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001774:Blood Chemical Analysis; D001794:Blood Pressure; D001802:Blood Substitutes / Q000737:chemistry / Q000378:metabolism*; D001831:Body Temperature; D004912:Erythrocytes / Q000378:metabolism; D005078:Exchange Transfusion, Whole Blood; D006339:Heart Rate; D006418:Heme / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000418:Albumins; D001802:Blood Substitutes; D011994:Recombinant Proteins; D006418:Heme; D010100:Oxygen