title: Malassezia sympodialis stimulation differently affects gene expression in dendritic cells from atopic dermatitis patients and healthy individuals.
abstract: It is known that 28-84% of patients with atopic dermatitis exhibit IgE and/or T-cell reactivity to the opportunistic yeast Malassezia sympodialis, which can be taken up by immature monocyte-derived dendritic cells (MDDCs), resulting in MDDC maturation. The aim of this study was to investigate whether MDDCs from patients with atopic dermatitis respond differently to M. sympodialis compared to MDDCs from healthy individuals. Immature MDDCs were stimulated with M. sympodialis and the gene expression profiles were analysed with cDNA arrays containing 406 genes. Our results show that M. sympodialis differently affected MDDCs from patients with atopic dermatitis, and more so in severely ill patients, compared with healthy individuals. Six genes were more than fivefold up-regulated in MDDCs from more than one patient with atopic dermatitis, coding for CD54, CD83, IL-8, monocyte-derived chemokine (MDC), BTG1 and IL-1R antagonist. In healthy individuals this was true only for BTG1. Up-regulations of IL-8 and MDC were confirmed at the protein level. Our findings might reflect an increased trafficking and stimulatory capacity in MDDCs from the patients, which is likely to result in a stronger inflammatory response to M. sympodialis.
authors.affiliation: Department of Medicine, Unit of Clinical Allergy Research, Karolinska Institutet and Hospital, Stockholm, Sweden. Susanne.Gabrielsson@ks.se
authors.forename: Susanne
authors.initials: S
authors.lastname: Gabrielsson
authors.forename: Eva
authors.initials: E
authors.lastname: Buentke
authors.forename: Agne
authors.initials: A
authors.lastname: Liedén
authors.forename: Margit
authors.initials: M
authors.lastname: Schmidt
authors.forename: Mauro
authors.initials: M
authors.lastname: D'Amato
authors.forename: Maria
authors.initials: M
authors.lastname: Tengvall-Linder
authors.forename: Annika
authors.initials: A
authors.lastname: Scheynius
mesh_terms: D000328:Adult; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology*; D003881:Dermatomycoses / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008290:Malassezia / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E