title: Detection of kaposi sarcoma-associated herpesvirus DNA in saliva and buffy-coat samples from children with sickle cell disease in Uganda.
abstract: Among 233 children, Kaposi sarcoma-associated herpesvirus (KSHV) DNA was detected in 43% of children seropositive for both K8.1 and orf73, in 29% of children seropositive for K8.1 only, in 14% of children seropositive for orf73 only, and in 7% of children seronegative for both K8.1 and orf73; among 228 mothers, KSHV DNA was detected in 27%, 25%, 4%, and 1%, respectively. KSHV DNA was detected more frequently and at higher levels in saliva than in buffy-coat samples and in children than in mothers. In both children and mothers, detection in saliva was associated with detection in peripheral blood. Detection was associated with K8.1 seropositivity, younger age, and high household density, indicating the importance of in-household person-to-person transmission, likely via saliva.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Rockville, Maryland, USA. mbulaits@mail.nih.gov.
authors.forename: Sam M
authors.initials: SM
authors.lastname: Mbulaiteye
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Pfeiffer
authors.forename: Eric A
authors.initials: EA
authors.lastname: Engels
authors.forename: Vickie
authors.initials: V
authors.lastname: Marshall
authors.forename: Paul M
authors.initials: PM
authors.lastname: Bakaki
authors.forename: Anchilla M
authors.initials: AM
authors.lastname: Owor
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Ndugwa
authors.forename: Edward
authors.initials: E
authors.lastname: Katongole-Mbidde
authors.forename: James J
authors.initials: JJ
authors.lastname: Goedert
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Biggar
authors.forename: Denise
authors.initials: D
authors.lastname: Whitby
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000755:Anemia, Sickle Cell / Q000097:blood / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D018562:Disease Transmission, Infectious; D005260:Female; D006566:Herpesviridae Infections / Q000097:blood / Q000635:transmission* / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000821:virology*; D014454:Uganda
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral