title: Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: the use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction.
abstract: Simultaneous comparison of differentially expressed protein profiles of Prorocentrum triestinum grown under different growth phases and growth conditions indicated the presence of phase-specific and stress-responsive proteins, respectively. Correlation studies on these proteins in relation to cell division phasing patterns and to models of phytoplankton growth inferred the possible functions. Most notable among these proteins were groups of proteins thought to trigger or mediate cells through specific phases of division of this alga, e.g., BP1, BP2, PB1, PB2, and PB3. Other proteins (e.g., group 1 proteins) thought to be responsible for maintaining and supporting cell concentration under adverse conditions were found. Furthermore, another group of proteins (group 2 proteins) thought to be stress-responsive were also detected. Taken overall, these differentially expressed proteins provided important information for uncovering various protective and adaptive mechanisms in the dinoflagellate's life cycle. These proteins have the potential to serve as "indicator proteins" for rapid assessment of the nutritional or metabolic status of these phytoplankton cells,and monitoring the differential expression of these phase-specific proteins and stress-specific proteins could be an important biomarker for bloom prediction.
authors.affiliation: Department of Ecology and Biodiversity, The University of Hong Kong, HKSAR, PR China.
authors.forename: Leo Lai
authors.initials: LL
authors.lastname: Chan
authors.forename: Ivor John
authors.initials: IJ
authors.lastname: Hodgkiss
authors.forename: Jennifer Man-Fan
authors.initials: JM
authors.lastname: Wan
authors.forename: John Hon-Kei
authors.initials: JH
authors.lastname: Lum
authors.forename: Abby Sin-Chi
authors.initials: AS
authors.lastname: Mak
authors.forename: Wai-Hung
authors.initials: WH
authors.lastname: Sit
authors.forename: Samuel Chun-Lap
authors.initials: SC
authors.lastname: Lo
mesh_terms: D020418:Algal Proteins / Q000737:chemistry*; D002453:Cell Cycle; D015180:Electrophoresis, Gel, Two-Dimensional; D056890:Eukaryota / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D020418:Algal Proteins; D020543:Proteome