title: Severe Chlamydophila psittaci pneumonia rapidly diagnosed by detection of antigen in sputum with an immunochromatography assay.
abstract: We report a case of severe Chlamydophila (Chlamydia) psittaci pneumonia rapidly diagnosed by detection of antigen in sputum with an immunochromatography assay. The patient was admitted to our hospital because of shock, disturbance of consciousness, accidental hypothermia, and multiple organ dysfunction syndrome, and he recovered after administration of intravenous erythromycin and high-dose methylpredonisolone therapy. Psittacosis was confirmed by detection of 16S rRNA gene of C. psittasi in sputum with multiplex-polymerase chain reaction analysis. Serological responses to C. psittasi, C. trachomatis, and C. pneumoniae were also evaluated, and serological cross-reactivity was observed between each species. We consider that the commercially available immunochromatography assay for Chlamydia species can be helpful for rapid diagnosis of Chlamydia infection of the respiratory tract. Hereafter, further examination will be necessary regarding pretreatment of specimens or detection sensitivity and specificity.
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Osaka University, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan. toyokawa@hp-lab.med.osaka-u.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Toyokawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ogawa
authors.forename: Sadashi
authors.initials: S
authors.lastname: Shiga
authors.forename: Isao
authors.initials: I
authors.lastname: Nishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Hosotsubo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Horikawa
authors.forename: Seishi
authors.initials: S
authors.lastname: Asari
mesh_terms: D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis*; D002691:Chlamydophila psittaci / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007159:Immunologic Tests; D008297:Male; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D009956:Psittacosis / Q000175:diagnosis* / Q000382:microbiology; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial