title: Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001.
abstract: Partial 1D gene characterization was used to study phylogenetic relationships between 17 serotype O foot-and-mouth disease (FMD) viruses in Ethiopia as well as with other O-type isolates from Eritrea, Kenya, South and West Africa, the Middle East, Asia and South America. A homologous region of 495 bp corresponding to the C-terminus end of the 1D gene was used for phylogenetic analysis. This study described three lineages, viz. African/Middle East-Asia, Cathay and South American. Within lineage I, three topotypes were defined, viz. East and West Africa and the Middle East-Asia together with the South African isolate. The Ethiopian isolates clustered as part of topotype I, the East African topotype. Two clades (based on < 12 % nucleotide difference) A and B were identified within the East African isolates, with clade A being further classified into three significant branches, A1 (80% bootstrap support), A2 (89% bootstrap support) and A3 (94% bootstrap support). Clade B consisted of two Kenyan isolates. Within topotype I, the 17 Ethiopian isolates showed genetic heterogeneity between themselves with sequence differences ranging from 4.6-14 %. Lineage 2 and 3 could be equated to two significant topotypes, viz. Cathay and South America. Comparison of amino acid variability at the immunodominant sites between the vaccine strain (ETH/19/77) and other Ethiopian outbreak isolates revealed variations within these sites. These results encourage further work towards the reassessment of the type O vaccine strain currently being used in Ethiopia to provide protection against field variants of the virus.
authors.affiliation: Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Sahle
authors.forename: E H
authors.initials: EH
authors.lastname: Venter
authors.forename: R M
authors.initials: RM
authors.lastname: Dwarka
authors.forename: W
authors.initials: W
authors.lastname: Vosloo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005002:Ethiopia / Q000453:epidemiology; D005536:Foot-and-Mouth Disease / Q000453:epidemiology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013045:Species Specificity
chemical_list: D004279:DNA, Viral