title: Acute bovine laminitis: a new induction model using alimentary oligofructose overload.
abstract: Twelve dairy heifers were used to examine the clinical response of an alimentary oligofructose overload. Six animals were divided into 3 subgroups, and each was given a bolus dose of 13, 17, or 21 g/kg of oligofructose orally. The control group (n = 6) was sham-treated with tap water. Signs of lameness, cardiovascular function, and gastrointestinal function were monitored every 6 h during development of rumen acidosis. The heifers were euthanized 48 and 72 h after administration of oligofructose. All animals given oligofructose developed depression, anorexia, and diarrhea 9 to 39 h after receiving oligofructose. By 33 to 45 h after treatment, the feces returned to normal consistency and the heifers began eating again. Animals given oligofructose developed transient fever, severe metabolic acidosis, and moderate dehydration, which were alleviated by supportive therapy. Four of 6 animals given oligofructose displayed clinical signs of laminitis starting 39 to 45 h after receiving oligofructose and lasting until euthanasia. The lameness was obvious, but could easily be overlooked by the untrained eye, because the heifers continued to stand and walk, and did not interrupt their eating behavior. No positive pain reactions or lameness were seen in control animals. Based on these results, we conclude that an alimentary oligofructose overload is able to induce signs of acute laminitis in cattle. This model offers a new method, which can be used in further investigation of the pathogenesis and pathophysiology of bovine laminitis.
authors.affiliation: Department of Large Animal Sciences, Clinical Institute, Royal Veterinary and Agricultural University of Copenhagen, Denmark. mbt@kvl.dk
authors.forename: M B
authors.initials: MB
authors.lastname: Thoefner
authors.forename: C C
authors.initials: CC
authors.lastname: Pollitt
authors.forename: A W
authors.initials: AW
authors.lastname: Van Eps
authors.forename: G J
authors.initials: GJ
authors.lastname: Milinovich
authors.forename: D J
authors.initials: DJ
authors.lastname: Trott
authors.forename: O
authors.initials: O
authors.lastname: Wattle
authors.forename: P H
authors.initials: PH
authors.lastname: Andersen
mesh_terms: D000138:Acidosis / Q000662:veterinary; D000208:Acute Disease; D000818:Animals; D000855:Anorexia / Q000139:chemically induced / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology*; D003681:Dehydration / Q000139:chemically induced / Q000662:veterinary; D003967:Diarrhea / Q000139:chemically induced / Q000662:veterinary; D004195:Disease Models, Animal; D005260:Female; D005534:Foot Diseases / Q000139:chemically induced / Q000150:complications / Q000662:veterinary*; D007249:Inflammation / Q000139:chemically induced / Q000150:complications / Q000662:veterinary*; D007700:Kinetics; D007794:Lameness, Animal / Q000209:etiology; D009844:Oligosaccharides / Q000008:administration & dosage*; D012417:Rumen
chemical_list: D009844:Oligosaccharides; C120489:oligofructose