Nanocapsules: coating for living cells.
One of the most promising tools for future applications in science and medicine is the use of nanotechnologies. Especially self-assembly systems, e.g., polyelectrolyte (PE) capsules prepared by means of the layer-by-layer technique with tailored properties, fulfill the requirements for nano-organized systems in a satisfactory manner. The nano-organized shells are suitable as coating for living cells or artificial tissue to prevent immune response. With these shells, material can be delivered to predefined organs. In this paper, some preliminary results are presented, giving a broad overview over the possibilities to use nano-organized capsules. Based on the observations that the cells while duplicating break the capsule a mutant yeast strain (Saccharomyces cerevisiae), which express GFP-tubulin under galactose promotion, was investigated by means of confocal laser scanning microscopy. The measurements reveal an increased surface charge in the region of buds developed prior encapsulation. In order to test the used PE pair for cytotoxicity, germinating conidia of the fungi Neurospora crassa were coated. The investigation with fluorescence microscopy shows a variation in the surface charge for the growing region and the conidium poles. The capsules exhibit interesting properties as valuable tool in science and a promising candidate for application in the field of medicine.
Krol
Silke
S
National Institute for the Physics of Matter (INFM), Department of Physics, University of Genoa, Genoa 33-16146, Italy. krol@fisica.unige.it
Diaspro
Alberto
A
Magrassi
Raffaella
R
Ballario
Paola
P
Grimaldi
Benedetto
B
Filetici
Patrizia
P
Ornaghi
Prisca
P
Ramoino
Paola
P
Gliozzi
Alessandra
A
D000818:Animals; D001672:Biocompatible Materials; D002214:Capsules; D016503:Drug Delivery Systems; D049452:Green Fluorescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009492:Neurospora crassa; D010247:Paramecium; D011073:Polyamines; D011137:Polystyrenes; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000235:genetics
D001672:Biocompatible Materials; D002214:Capsules; D011073:Polyamines; D011137:Polystyrenes; D014404:Tubulin; D049452:Green Fluorescent Proteins; C063994:polyallylamine; C003321:polystyrene sulfonic acid