title: Cytokine gene polymorphisms in ischaemic heart disease: investigation using family-based tests of association.
abstract: Atherosclerosis has an inflammatory basis, with cytokines, cellular adhesion molecules and pro-inflammatory cells having important roles in the initiation and progression of this process. Interleukin (IL) 6, IL-10 and transforming growth factor (TGF) beta1 have been proposed as important modulators of the atherosclerotic process, with IL-6 having a pro-inflammatory, atherogenic effect and IL-10 and TGF-beta1 having anti-inflammatory, protective roles. The possible role of functional polymorphisms in the promoter regions of the IL-6, IL-10 and TGF-beta1 genes in the susceptibility to ischaemic heart disease (IHD) was investigated in a well-defined Irish population using two recently described family-based tests of association. We genotyped 1,012 individuals from 386 families with at least one member prematurely affected with IHD. Using the combined transmission disequilibrium test (TDT)/sib-TDT and the pedigree disequilibrium test, no association between any of the IL-6 -174G/C, IL-10 -1082G/A and TGF-beta1 -509C/T polymorphisms and IHD was found. Our data demonstrate that, in an Irish population, these polymorphisms are not associated with IHD.
authors.affiliation: Regional Medical Cardiology Centre, Royal Victoria Hospital, Grosvenor Road, BT12 6BA, Belfast, UK.
authors.forename: Paul G
authors.initials: PG
authors.lastname: McGlinchey
authors.forename: Mark S
authors.initials: MS
authors.lastname: Spence
authors.forename: Chris C
authors.initials: CC
authors.lastname: Patterson
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Allen
authors.forename: Gillian
authors.initials: G
authors.lastname: Murphy
authors.forename: David A
authors.initials: DA
authors.lastname: Savage
authors.forename: A Peter
authors.initials: AP
authors.lastname: Maxwell
authors.forename: Pascal P
authors.initials: PP
authors.lastname: McKeown
mesh_terms: D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic
chemical_list: D016207:Cytokines