Mapping herpes simplex virus type 1 latency-associated transcript sequences that protect from apoptosis mediated by a plasmid expressing caspase-8.
LAT (latency-associated transcript) is the only herpes simplex virus type 1 (HSV-1) transcript abundantly expressed during neuronal latency. LAT expression is required for the high reactivation phenotype of HSV-1 and this phenotype correlates with LAT's anti-apoptosis properties. LAT nucleotides 1 to 1499 inhibit caspase-8 (death receptor apoptotic pathway), but not caspase-9 (mitochondrial apoptotic pathway), -induced apoptosis as efficiently as larger LAT fragments. LAT sequences important for inhibiting caspase-8-induced apoptosis were also localized. The ability of LAT nucleotides 1 to 1499 to efficiently inhibit caspase-8-induced apoptosis correlates with the high reactivation phenotype of a mutant virus expressing just the first 1.5 kb of LAT (nucleotides 1 to 1499).
Peng
W
W
Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska, Lincoln 68583-0905, USA.
Jin
L
L
Henderson
G
G
Perng
G C
GC
Brick
D J
DJ
Nesburn
A B
AB
Wechsler
S L
SL
Jones
C
C
D000818:Animals; D017209:Apoptosis; D053181:Caspase 8; D020169:Caspases / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014158:Transcription, Genetic; D017735:Virus Latency / Q000235:genetics*
C505530:CASP8 protein, human; D053181:Caspase 8; D020169:Caspases