Lipid raft proteins and their identification in T lymphocytes.
This review focuses on how membrane lipid rafts have been detected and isolated, mostly from lymphocytes, and their associated proteins identified. These proteins include transmembrane antigens/receptors, GPI-anchored proteins, cytoskeletal proteins, Src-family protein kinases, G-proteins, and other proteins involved in signal transduction. To further understand the biology of lipid rafts, new methodological approaches are needed to help characterize the raft protein component, and changes that occur in this component as a result of cell perturbation. We describe the application of new proteomic approaches to the identification and quantification of raft proteins in T-lymphocytes. Similar approaches, applied to other model cell systems, will provide valuable new insights into both cellular signal transduction and lipid raft biology.
Wollscheid
Bernd
B
Institute for Systems Biology, Seattle, WA 98103, USA.
von Haller
Priska D
PD
Yi
Eugene
E
Donohoe
Samuel
S
Vaughn
Kelly
K
Keller
Andrew
A
Nesvizhskii
Alexey I
AI
Eng
Jimmy
J
Li
Xiao-jun
XJ
Goodlett
David R
DR
Aebersold
Ruedi
R
Watts
Julian D
JD
D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology; D017261:Glycosylphosphatidylinositols / Q000502:physiology; D006801:Humans; D021962:Membrane Microdomains / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D020543:Proteome; D013601:T-Lymphocytes / Q000648:ultrastructure*
D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D020543:Proteome; D011505:Protein-Tyrosine Kinases; D019204:GTP-Binding Proteins