Neurogranin is expressed by principal cells but not interneurons in the rodent and monkey neocortex and hippocampus.
As a substrate of protein kinase C (PKC), neurogranin (NG) is involved in the regulation of calcium signaling and activity-dependent plasticity. Recently, we have shown that, in the rodent cerebellum, NG is exclusively expressed by gamma-aminobutyric acidergic Golgi cells, whereas, in the monkey cerebellum, brush cells were the only neuronal population expressing NG (Singec et al. [2003] J. Comp. Neurol. 459:278-289). In the present study, we analyzed the neocortical and hippocampal expression patterns of NG in adult mouse (C57Bl/6), rat (Wistar), and monkey (Cercopithecus aetiops). By using immunocytochemistry and nonradioactive in situ hybridization, we demonstrate strong NG expression by principal cells in different neocortical layers and in the hippocampus by granule cells of the dentate gyrus and pyramidal neurons of CA1-CA3. In contrast, double-labeling experiments in rodents revealed that neocortical and hippocampal interneurons expressing glutamate decarboxylase 67 (GAD67) were consistently devoid of NG. In addition, by using antibodies against parvalbumin, calbindin, and calretinin, we could demonstrate the absence of NG in interneurons of monkey frontal cortex and hippocampus. Together these findings corroborate the idea of different calcium signaling pathways in excitatory and inhibitory cells that may contribute to different modes of synaptic plasticity in these neurons.
Singec
Ilyas
I
Institute of Anatomy and Cell Biology, University of Freiburg, D-79104 Freiburg, Germany. singeci@ninds.nih.gov
Knoth
Rolf
R
Ditter
Margarethe
M
Volk
Benedikt
B
Frotscher
Michael
M
D000818:Animals; D020013:Calcium Signaling / Q000502:physiology; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007395:Interneurons / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009433:Neural Inhibition / Q000502:physiology; D051579:Neurogranin; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D009435:Synaptic Transmission / Q000502:physiology
D002148:Calmodulin-Binding Proteins; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; C496650:Nrgn protein, mouse; C496651:Nrgn protein, rat; D051579:Neurogranin; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1