title: [Effect of anti-human VEGF monoclonal antibody on VEGF level in serum of nude mice transplanted buccal carcinoma: a quantitative study].
abstract: OBJECTIVE
  Quantitative study of the effect of anti-human VEGF mAb E11 to VEGF level in serum of nude mice transplanted buccal carcinoma.
  
  
  METHODS
  E11 was administered into BALB/c nu/nu mice which were transplanted human buccal carcinoma. The saline was administrated as negative control. Mice were killed at 18 days. The VEGF level in serum of mice was determined by improved indirect ELISA.
  
  
  RESULTS
  Compared with the VEGF level in serum of mice in saline group, it was dramatically decreased in E11 group. The VEGF level in serum of mice treated E11 by subcutaneous was lowest and only reached (1.17 +/- 0.13) microg/L.
  
  
  CONCLUSIONS
  It demonstrated that the anti-human VEGF mAb could reduce the VEGF level in serum by binding VEGF, and block its biological activity. It indicates that VEGF in serum of malignant tumor patient is a new tumor marker.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, the Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai 519000, China.
authors.forename: Si-lian
authors.initials: SL
authors.lastname: Fang
authors.forename: Da-zhang
authors.initials: DZ
authors.lastname: Wang
authors.forename: Jing-yi
authors.initials: JY
authors.lastname: Zhang
authors.forename: Guang-yong
authors.initials: GY
authors.lastname: Zheng
authors.forename: Xi-chuan
authors.initials: XC
authors.lastname: Yang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000097:blood; D002277:Carcinoma / Q000097:blood*; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000473:pathology*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse