PCR-based capsular serotype determination of Haemophilus influenzae strains recovered from Japanese paediatric patients with invasive infection.
The serotypes of 53 isolates of Haemophilus influenzae from children with invasive infections were determined by a conventional slide agglutination test (SAT) and a recently proposed PCR-based method for serotyping H. influenzae. The PCR assay identified 47 (88.7%) type b isolates, one (1.9%) type e isolate and five (9.4%) non-typeable isolates. The only discrepancy between the methods was an isolate that was non-typeable by SAT, but was identified as serotype e by PCR. Of 41 isolates from patients with meningitis, 39 (95.1%) were type b. Of the five non-typeable isolates, three (60%) were from the blood of patients with septicaemic pneumonia and two (40%) were from the cerebrospinal fluid of patients with meningitis. None of the non-typeable isolates appeared to be a capsule-deficient mutant of an encapsulated H. influenzae strain. Overall, the study confirmed the usefulness of this PCR method for the serotyping of invasive H. influenzae isolates.
Ishiwada
N
N
Department of Paediatrics, Graduate School of Medicine, Chiba University, Chiba, Chuo-ku, Inohana, Japan. ishiwada@faculty.chiba-u.ac.jp
Cao
L D
LD
Kohno
Y
Y
D016667:Bacterial Capsules; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D018073:Haemophilus Vaccines / Q000737:chemistry / Q000235:genetics*; D019741:Haemophilus influenzae type b / Q000145:classification* / Q000235:genetics; D006801:Humans; D007223:Infant; D007564:Japan; D008297:Male; D008583:Meningitis, Haemophilus / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000235:genetics*; D012703:Serotyping / Q000379:methods
D004269:DNA, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial