title: Implications of fungicidal effects of benzimidazole compounds on Duddingtonia flagrans in integrated nematode parasite management in livestock.
abstract: An in vitro trial with carbendazim fungicide on the growth profile of the predatory fungus Duddingtonia flagrans was undertaken and in vivo trials in sheep and buffaloes, fed on chlamydospores of D. flagrans and administered albendazole anthelmintic, were conducted. Although no growth inhibition was detected at a carbendazim concentration of 0.05 ppm, growth inhibition was recorded of 50% and above at concentrations of 0.25 and 1.00 ppm (p < 0.001) and of around 90% at concentrations of 2.00 to 5.00 ppm (p <0.0001). Scanty recovery of the fungus was made from faecal culture 48 h following a single dose of albendazole both in sheep and buffaloes. However, profuse fungal recovery was made from 96 h post dosing onwards. When the drug was used as an intraruminal slow-release capsule, no faecal fungal recovery could be made from day 3 after administration of the capsule, when the albendazole sulphoxide concentration was around 1.0 microg/ml. However, profuse and scanty fungal recovery could be made on days 1 and 2, respectively, after administration of the capsule, when the plasma albendazole sulphoxide concentration was around 0.4 and 0.9 microg/ml, respectively. The implications for use of a combination of anthelmintics and biological control in sustainable parasite control programmes are discussed.
authors.affiliation: Biotechnology Laboratory, National Dairy Development Board, Anand, Gujarat, India. sanyalpk@indiatimes.com
authors.forename: P K
authors.initials: PK
authors.lastname: Sanyal
authors.forename: J B
authors.initials: JB
authors.lastname: Chauhan
authors.forename: P N
authors.initials: PN
authors.lastname: Mukhopadhyaya
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000969:Antinematodal Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D001203:Ascomycota / Q000737:chemistry; D001562:Benzimidazoles / Q000302:isolation & purification* / Q000627:therapeutic use*; D002020:Buffaloes; D002219:Carbamates / Q000302:isolation & purification* / Q000627:therapeutic use; D009348:Nematoda / Q000382:microbiology; D009349:Nematode Infections / Q000188:drug therapy / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*
chemical_list: D000969:Antinematodal Agents; D001562:Benzimidazoles; D002219:Carbamates; C006698:carbendazim