title: Sevoflurane anesthesia attenuates adenosine diphosphate-induced P-selectin expression and platelet-leukocyte conjugate formation.
abstract: The expression of P-selectin on the surface of platelets and platelet-leukocyte conjugate formation are considered to be an indicator of platelet activation and are important in thrombotic and inflammatory disease. Previous studies have reported the inhibitory effects of sevoflurane on platelet aggregation. We investigated whether sevoflurane alters the expression of P-selectin on platelets and the formation of platelet-leukocyte conjugates. Twenty-five patients undergoing minor extremity surgery received sevoflurane-based general anesthesia, with mask induction and laryngeal mask airway anesthesia maintenance. Whole blood was obtained before and 40 min after sevoflurane anesthesia. Unstimulated and adenosine diphosphate-stimulated samples of whole blood and platelet rich plasma were stained with fluorochrome-conjugated antibodies. The expression of P-selectin on platelets and the formation of platelet-leukocyte conjugates were measured using flow cytometry. Sevoflurane inhibited platelet P-selectin expression. It also reduced the formation of platelet-leukocyte conjugates, both in unstimulated and adenosine diphosphate-stimulated blood samples at 3%-4% end-expiratory sevoflurane concentrations used to maintain anesthesia.
authors.affiliation: *Department of Anesthesiology and †Division of Cardiovascular Surgery, Tri-Service General Hospital, National Defense Medical Center, National Defense University, Taipei, Taiwan, Republic of China.
authors.forename: Go-Shine
authors.initials: GS
authors.lastname: Huang
authors.forename: Chi-Yuan
authors.initials: CY
authors.lastname: Li
authors.forename: Ping-Ching
authors.initials: PC
authors.lastname: Hsu
authors.forename: Chien-Sung
authors.initials: CS
authors.lastname: Tsai
authors.forename: Tso-Chou
authors.initials: TC
authors.lastname: Lin
authors.forename: Chih-Shung
authors.initials: CS
authors.lastname: Wong
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000328:Adult; D000769:Anesthesia, Inhalation; D001792:Blood Platelets / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008738:Methyl Ethers; D019007:P-Selectin / Q000096:biosynthesis*; D000077149:Sevoflurane
chemical_list: D008738:Methyl Ethers; D019007:P-Selectin; D000077149:Sevoflurane; D000244:Adenosine Diphosphate