title: [Proacrosin, an acrosomal marker for the detection of spermatogenic cells in ejaculates from azoospermic men].
abstract: Between January 2003 and April 2004, a prospective study was performed on ejaculates from non-obstructive azoospermic men (n = 95), for the identification of spermatogenic cells using an immunohistochemical labeling for proacrosin. 48.4% of ejaculates (46/95) displayed labeled spermatogenic cells. A 38/95 (40%) of men had testicular sperm extraction (TESE) followed by intracytoplasmic sperm injection (i.c.s.i.). Testicular spermatozoa were extracted from 21 men (52.5%). The sensitivity of detecting spermatogenic cells is 66.7% in predicting the presence of testicular spermatozoa, and its specificity is 76.5%. Compared to histopathological diagnostic testicular biopsy, the detection of spermatogenic cells using proacrosin immunohistochemical method offers a predictive parameter for successful TESE. The immunohistochemical method for proacrosin has the advantages of simplicity and low cost. It could be used to predict spermatogenesis from non-obstructive azoospermic men. Further evaluation is required with extensive results to improve sensitivity and specificity.
authors.affiliation: Unité fonctionnelle de spermiologie, hôpital Calmette, RDJ-1 Ouest, bd du Pr.-Jules-Leclerq, 59037 Lille, France. v-mitchell@chru-lille.fr
authors.forename: V
authors.initials: V
authors.lastname: Mitchell
authors.forename: S
authors.initials: S
authors.lastname: Brabant
authors.forename: I
authors.initials: I
authors.lastname: Koscinski
authors.forename: E
authors.initials: E
authors.lastname: Hermand
authors.forename: J-M
authors.initials: JM
authors.lastname: Rigot
mesh_terms: D000176:Acrosin / Q000032:analysis*; D000177:Acrosome / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D004792:Enzyme Precursors / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D020554:Sperm Injections, Intracytoplasmic; D013091:Spermatogenesis; D013094:Spermatozoa / Q000737:chemistry*; D020858:Tissue and Organ Harvesting
chemical_list: D015415:Biomarkers; D004792:Enzyme Precursors; C022438:proacrosin; D000176:Acrosin
vernacular_title: La proacrosine, un marqueur acrosomal pour la détection des cellules spermatiques dans le sperme éjaculé de patients azoospermes.