title: Neither single-marker nor haplotype analyses support an association between genetic variation near NOTCH4 and bipolar disorder.
abstract: Markers near the NOTCH4 locus on chromosome 6p21.3 have been reported to be associated with schizophrenia in some studies. Since schizophrenia and bipolar affective disorder (BPAD) may share genetic determinants, we tested markers in and near NOTCH4 in a sample of 153 parent-offspring triads ascertained through a sibling pair with BPAD for evidence of association. This sample would have 80% power to detect an association at or above a genotype relative risk of 2.4 at the 10(-7) level of significance. In addition to the two markers previously showing the most significant association with schizophrenia, three additional nearby markers were studied. The five markers were genotyped using validated methods. Both single-marker and 3-marker haplotype data was analyzed using family-based association methods. No genome-wide significant association was detected between any of the five SNP-markers and BPAD in this sample. One marker showed nominal evidence of association (P = 0.049), but this evidence was not supported by haplotype analyses including nearby flanking markers or by case-control analysis using 93 Caucasian controls. These results do not support an association between genetic variation near NOTCH4 and BPAD in this sample.
authors.affiliation: Department of Psychiatry, University of Chicago, Chicago, Illinois 60615, USA. sprathik@yoda.bsd.uchicago.edu
authors.forename: Sridhar
authors.initials: S
authors.lastname: Prathikanti
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Schulze
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Bettina
authors.initials: B
authors.lastname: Harr
authors.forename: Nirmala
authors.initials: N
authors.lastname: Akula
authors.forename: Kathy
authors.initials: K
authors.lastname: Hennessy
authors.forename: Suneeta
authors.initials: S
authors.lastname: Potluri
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lyons
authors.forename: Tu
authors.initials: T
authors.lastname: Nguyen
authors.forename: Francis J
authors.initials: FJ
authors.lastname: McMahon
mesh_terms: D000483:Alleles; D001714:Bipolar Disorder / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D000074562:Receptor, Notch4; D011956:Receptors, Cell Surface / Q000235:genetics*; D051880:Receptors, Notch; D018911:Trinucleotide Repeats / Q000235:genetics
chemical_list: D005819:Genetic Markers; C106075:NOTCH4 protein, human; D011518:Proto-Oncogene Proteins; D000074562:Receptor, Notch4; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; D004247:DNA