title: SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays.
abstract: A histone variant H2ABbd was recently identified, but its function is totally unknown. Here we have studied the structural and functional properties of nucleosome and nucleosomal arrays reconstituted with this histone variant. We show that H2ABbd can replace the conventional H2A in the nucleosome, but this replacement results in alterations of the nucleosomal structure. The remodeling complexes SWI/SNF and ACF are unable to mobilize the variant H2ABbd nucleosome. However, SWI/SNF was able to increase restriction enzyme access to the variant nucleosome and assist the transfer of variant H2ABbd-H2B dimer to a tetrameric histone H3-H4 particle. In addition, the p300- and Gal4-VP16-activated transcription appeared to be more efficient for H2ABbd nucleosomal arrays than for conventional H2A arrays. The intriguing mechanisms by which H2ABbd affects both nucleosome remodeling and transcription are discussed.
authors.affiliation: Ecole Normale Supérieure de Lyon, CNRS-UMR 5161, Lyon, France.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Angelov
authors.forename: André
authors.initials: A
authors.lastname: Verdel
authors.forename: Woojin
authors.initials: W
authors.lastname: An
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bondarenko
authors.forename: Fabienne
authors.initials: F
authors.lastname: Hans
authors.forename: Cécile-Marie
authors.initials: CM
authors.lastname: Doyen
authors.forename: Vassily M
authors.initials: VM
authors.lastname: Studitsky
authors.forename: Ali
authors.initials: A
authors.lastname: Hamiche
authors.forename: Robert G
authors.initials: RG
authors.lastname: Roeder
authors.forename: Philippe
authors.initials: P
authors.lastname: Bouvet
authors.forename: Stefan
authors.initials: S
authors.lastname: Dimitrov
mesh_terms: D000107:Acetylation; D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I; D019281:Dimerization; D006657:Histones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000502:physiology*; D009707:Nucleosomes / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014981:Xenopus / Q000502:physiology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C070633:Gal-VP16; D006657:Histones; D009687:Nuclear Proteins; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; C445179:SWI-SNF-B chromatin-remodeling complex; D015534:Trans-Activators; D014157:Transcription Factors; D003850:Deoxyribonuclease I