Effects of genetic and nutritional factors on bone mineral density in young adults.
To estimate the genetic and dietary factors influencing bone mineral density (BMD) in young adults, a total of 53 healthy volunteers (HV) (age 20.89+/-1.34), from whom informed consent was obtained, answered a questionnaire on dietary factors and had DNA from peripheral blood mononuclear cells analyzed for single nucleotide polymorphisms (SNPs) for vitamin (Vit) D receptor (VDR), estrogen receptor alpha (ERalpha), interleukin 1 receptor antagonist (IL1RA), and apolipoprotein E (ApoE) genes. Daily intakes of Vit C, fiber, soybean and related foods, and green and yellow vegetables showed a correlation with % BMD. In addition, Vit B2 as well as Vit C, and vegetables were identified as important factors for BMD by Stepwise regression analysis. Among the SNPs analyzed, the B+ type of the VDR gene tended to be associated with a lower BMD, and pp type of the ER gene digested by the PvuII enzyme in females indicated a significantly lower BMD than that in males. In addition, these SNPs were also identified by factor analysis to be associated with BMD. These results suggested that a complex array of genetic factors, such as two or more SNPs or SNPs and gender, may be important to BMD.
Otsuki
Takemi
T
Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan. takemi@med.kawasaki-m.ac.jp
Sakaguchi
Haruko
H
Hatta
Emiko
E
Hatayama
Tamayo
T
Hatada
Satomi
S
Miura
Yoshie
Y
Takata-Tomokuni
Akiko
A
Hyodoh
Fuminori
F
Tomomitsu
Tatsushi
T
Fukunaga
Masao
M
Katsuyama
Hironobu
H
D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics; D015519:Bone Density / Q000235:genetics* / Q000502:physiology*; D004032:Diet; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D016014:Linear Models; D008297:Male; D009747:Nutritional Physiological Phenomena; D009752:Nutritional Status / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics; D012727:Sex Characteristics; D012795:Sialoglycoproteins / Q000235:genetics; D011795:Surveys and Questionnaires
D001057:Apolipoproteins E; D047628:Estrogen Receptor alpha; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D018167:Receptors, Calcitriol; D012795:Sialoglycoproteins