title: Increased messenger RNA expression of vascular endothelial growth factor and its receptors in the implantation site of the human oviduct with ectopic gestation.
abstract: OBJECTIVE
  To compare the mRNA expression of vascular endothelial growth factor (VEGF) and its receptors (KDR and flt-1) in the implantation and nonimplantation sites of the human oviduct with ectopic gestation.
  
  
  METHODS
  Prospective observational study.
  
  
  METHODS
  University-based Obstetrics and Gynecology Department.
  
  
  METHODS
  Ten women undergoing laparoscopic salpingectomy for tubal pregnancy.
  
  
  METHODS
  The mucosal layer was isolated from the implantation and nonimplantation sites of the oviduct tissue with ectopic gestation. Semiquantitative reverse transcriptase-polymerase chain reaction was performed.
  
  
  METHODS
  The differences in the mRNA expression of VEGF and its receptors between the implantation and nonimplantation sites of the oviduct tissue.
  
  
  RESULTS
  The mRNA expression of VEGF and its receptors, both KDR and flt-1, was significantly higher in the implantation site of the human oviduct with ectopic gestation compared with the nonimplantation site.
  
  
  CONCLUSIONS
  The results suggest that VEGF may be the angiogenic factor responsible for the implantation and placentation of an ectopic pregnancy in the oviduct.
authors.affiliation: Department of Obstetrics and Gynecology, Prince of Wales Hospital Shatin, Hong Kong SAR, China. lampomui@cuhk.edu.hk
authors.forename: Po Mui
authors.initials: PM
authors.lastname: Lam
authors.forename: Christine
authors.initials: C
authors.lastname: Briton-Jones
authors.forename: Che Kwok
authors.initials: CK
authors.lastname: Cheung
authors.forename: See Wai
authors.initials: SW
authors.lastname: Leung
authors.forename: Lai Ping
authors.initials: LP
authors.lastname: Cheung
authors.forename: Christopher
authors.initials: C
authors.lastname: Haines
mesh_terms: D006063:Chorionic Gonadotropin / Q000097:blood; D010064:Embryo Implantation; D004958:Estradiol / Q000097:blood; D005187:Fallopian Tubes / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000235:genetics; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics*; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D011374:Progesterone; D004958:Estradiol; D040262:Receptors, Vascular Endothelial Growth Factor