Screen for genes differentially expressed during regeneration of the zebrafish caudal fin.
The zebrafish caudal fin constitutes an important model for studying the molecular basis of tissue regeneration. The cascade of genes induced after amputation or injury, leading to restoration of the lost fin structures, include those responsible for wound healing, blastema formation, tissue outgrowth, and patterning. We carried out a systematic study to identify genes that are up-regulated during "initiation" (1 day) and "outgrowth and differentiation" (4 days) of fin regeneration by using two complementary methods, suppression subtraction hybridization (SSH) and differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). We obtained 298 distinct genes/sequences from SSH libraries and 24 distinct genes/sequences by DDRT-PCR. We determined the expression of 54 of these genes using in situ hybridization. In parallel, gene expression analyses were done in zebrafish embryos and early larvae. The information gathered from the present study provides resources for further investigations into the molecular mechanisms of fin development and regeneration.
Padhi
Bhaja K
BK
Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa K1Y 4E9, Ontario, Canada.
Joly
Lucille
L
Tellis
Patricia
P
Smith
Amanda
A
Nanjappa
Purushothama
P
Chevrette
Mario
M
Ekker
Marc
M
Akimenko
Marie-Andrée
MA
D000671:Amputation, Surgical; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004625:Embryo, Nonmammalian; D020224:Expressed Sequence Tags; D005121:Extremities / Q000196:embryology* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005820:Genetic Testing; D017403:In Situ Hybridization; D007633:Keratins / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000502:physiology
D018076:DNA, Complementary; D007633:Keratins