title: Initial interaction of the P22 phage with the Salmonella typhimurium surface.
abstract: OBJECTIVE
  The goals of these studies were to characterize the interaction of the P22 phage particle with the Salmonella cell surface and to determine the phage elements involved in this interaction by mutational analysis.
  
  
  BACKGROUND
  The phage P22 has been characterized extensively. The gene and protein for the phage P22 tailspike, which is the phage adsorption organelle, have been intensively studied. The kinetics of the interaction of the tailspike protein with the cell surface has been studied in detail, surprisingly no mutational analysis has ever been reported that has defined these components and their interaction between themselves and the cell surface. The main and perhaps only component needed for this cell surface interaction is the tailspike protein.
  
  
  METHODS
  Adsorption to the cell surface has been measured in the wild type phage and in mutant derivatives, isolated in this study. Phage mutants have been isolated after hydroxylamine mutagenesis.
  
  
  RESULTS
  The adsorption of P22 to the cell surface is a temperature-independent event. Forty putative phage adsorption mutants have been isolated. A sample of them have been further analyzed. These divide the adsorption process into at least two stages. One stage contains mutants that absorb with essential wild type phage kinetics to the cell surface while the other stage with delayed adsorption kinetics.
  
  
  CONCLUSIONS
  The interaction of the phage P22 with the Salmonella cell surface has been shown to be a complicated one which is temperature-independent and multi-stage. Mutants isolated in this study may help dissect this process even further.
authors.affiliation: Universidad Central del Caribe School of Medicine, Department of Microbiology, Bayamón, Puerto Rico.
authors.forename: Celia Johanna
authors.initials: CJ
authors.lastname: Venza Colon
authors.forename: Andrea Y
authors.initials: AY
authors.lastname: Vasquez Leon
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Villafañe
mesh_terms: D000327:Adsorption; D017100:Bacteriophage P22 / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D013696:Temperature; D017376:Viral Tail Proteins / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D017376:Viral Tail Proteins