title: Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase.
abstract: Aromatic amines have been studied for more than a half-century as model carcinogens representing a class of chemicals that form bulky adducts to the C8 position of guanine in DNA. Among these guanine adducts, the N-(2'-deoxyguanosin-8-yl)-aminofluorene (G-AF) and N-2-(2'-deoxyguanosin-8-yl)-acetylaminofluorene (G-AAF) derivatives are the best studied. Although G-AF and G-AAF differ by only an acetyl group, they exert different effects on DNA replication by replicative and high-fidelity DNA polymerases. Translesion synthesis of G-AF is achieved with high-fidelity polymerases, whereas replication of G-AAF requires specialized bypass polymerases. Here we have presented structures of G-AF as it undergoes one round of accurate replication by a high-fidelity DNA polymerase. Nucleotide incorporation opposite G-AF is achieved in solution and in the crystal, revealing how the polymerase accommodates and replicates past G-AF, but not G-AAF. Like an unmodified guanine, G-AF adopts a conformation that allows it to form Watson-Crick hydrogen bonds with an opposing cytosine that results in protrusion of the bulky fluorene moiety into the major groove. Although incorporation opposite G-AF is observed, the C:G-AF base pair induces distortions to the polymerase active site that slow translesion synthesis.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Hsu
authors.forename: James R
authors.initials: JR
authors.lastname: Kiefer
authors.forename: Dominique
authors.initials: D
authors.lastname: Burnouf
authors.forename: Olivier J
authors.initials: OJ
authors.lastname: Becherel
authors.forename: Robert P P
authors.initials: RP
authors.lastname: Fuchs
authors.forename: Lorena S
authors.initials: LS
authors.lastname: Beese
mesh_terms: D000588:Amines / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D018736:DNA Adducts / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives*
chemical_list: D000588:Amines; D018736:DNA Adducts; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003849:Deoxyguanosine