title: Zooming in on a quantitative trait for tomato yield using interspecific introgressions.
abstract: To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.
authors.affiliation: Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Fridman
authors.forename: Fernando
authors.initials: F
authors.lastname: Carrari
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Liu
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Dani
authors.initials: D
authors.lastname: Zamir
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001224:Aspartic Acid; D020134:Catalytic Domain; D003433:Crosses, Genetic; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D005638:Fruit / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D032322:Solanum / Q000201:enzymology / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001224:Aspartic Acid; D013395:Sucrose; D043324:beta-Fructofuranosidase