[Experimantal study on the effect of ginsenosides of ginseng stem and leaf in enhancing the transactivation of glucocorticoid receptor induced by dexamethasone in vitro].
OBJECTIVE
To investigate the effect of ginsenosides of ginseng stem and leaf (GSL) on the transcription activity of glucocorticoid receptor (GR).


METHODS
HL7702 cells were transiently cotransfected by GR luciferase reporter plasmid pGRE-tk-Luc and inner referential gene pRL-SV40 plasmid, and the effects of dexamethasone (Dex) and GSL on the reporter gene expression were detected by dual-luciferase reporter assay.


RESULTS
The reporter gene expression Dex-induced in HL7702 was dose- and time-dependent, it could be multiplied to 93 times in maximum, and could be blocked by the GR antagonist RU486. GSL could not induce the expression of reporter gene but could elevate the induction of Dex for 61.4%.


CONCLUSIONS
GSL could enhance the inducing effect of Dex on transcription activity of GR in vitro.
Li
Yong
Y
Department of TCM, Changhai Hospital, the Second Military Medical University, Shanghai (200433).
Li
Min
M
Wang
Xi
X
D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology; D015735:Mifepristone / Q000494:pharmacology; D005894:Panax / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011965:Receptors, Glucocorticoid / Q000187:drug effects* / Q000502:physiology; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection
D036145:Ginsenosides; D011965:Receptors, Glucocorticoid; D015735:Mifepristone; D003907:Dexamethasone