title: Pistil induction by hormones from callus of Oryza sativa in vitro.
abstract: This study describes the successful formation of floral organ pistil from the callus of pistil explants of Oryza sativa L. For induction of floral organs, different explants--including young embryo, lemma, palea and pistil--were used for callus induction with different combinations of N(6)-benzyladenine and 2,4-dichlorophenoxyacetic acid (2,4-D). High frequencies of callus formation from pistil and young embryo explants were achieved. Floral organs were induced after calli from pistils were transferred to medium containing both zeatin and 2,4-D. The morphological characteristics of the pistil-like organs are very similar to those formed in planta though with minor differences. Further histological study revealed that the in vitro pistil contains an ovule within its ovary. Furthermore, a pistil-specific gene, OsMADS3 used as a molecular marker for pistil identity, was expressed in the pistil-like organs as it was in pistils in the flower of the plant.
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: An
authors.forename: X G
authors.initials: XG
authors.lastname: Li
authors.forename: H Y
authors.initials: HY
authors.lastname: Su
authors.forename: X S
authors.initials: XS
authors.lastname: Zhang
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D004627:Embryonic Induction / Q000502:physiology; D035264:Flowers / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D006540:Herbicides; D026161:MADS Domain Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D015084:2,4-Dichlorophenoxyacetic Acid