Quantitative proteomic analysis of metabolic regulation by copper ions in Methylococcus capsulatus (Bath).
Copper ions switch the oxidation of methane by soluble methane monooxygenase to particulate methane monooxygenase in Methylococcus capsulatus (Bath). Toward understanding the change in cellular metabolism related to this transcriptional and metabolic switch, we have undertaken genomic sequencing and quantitative comparative analysis of the proteome in M. capsulatus (Bath) grown under different copper-to-biomass ratios by cleavable isotope-coded affinity tag technology. Of the 682 proteins identified, the expressions of 60 proteins were stimulated by at least 2-fold by copper ions; 68 proteins were down-regulated by 2-fold or more. The 60 proteins overexpressed included the methane and carbohydrate metabolic enzymes, while the 68 proteins suppressed were mainly responsible for cellular signaling processes, indicating a role of copper ions in the expression of the genes associated with the metabolism of the organism downstream of methane oxidation. The study has also provided a complete map of the C1 metabolism pathways in this methanotroph and clarified the interrelationships between them.
Kao
Wei-Chun
WC
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Chen
Yet-Ran
YR
Yi
Eugene C
EC
Lee
Hookeun
H
Tian
Qiang
Q
Wu
Keh-Ming
KM
Tsai
Shih-Feng
SF
Yu
Steve S-F
SS
Chen
Yu-Ju
YJ
Aebersold
Ruedi
R
Chan
Sunney I
SI
D001426:Bacterial Proteins; D001669:Biochemical Phenomena; D001671:Biochemistry; D002241:Carbohydrates / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D019992:Databases as Topic; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007477:Ions; D008697:Methane / Q000737:chemistry; D020619:Methylococcus capsulatus / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002241:Carbohydrates; D007477:Ions; D010455:Peptides; D020543:Proteome; D003300:Copper; D008697:Methane; D010100:Oxygen