title: Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.
abstract: Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
authors.forename: A H
authors.initials: AH
authors.lastname: COONS
authors.forename: M H
authors.initials: MH
authors.lastname: KAPLAN
mesh_terms: D000906:Antibodies; D000941:Antigens; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins
chemical_list: D000906:Antibodies; D000941:Antigens; D007136:Immunoglobulins