Abnormal cell-specific expressions of certain protein kinase C isoenzymes in peripheral mononuclear cells of patients with systemic lupus erythematosus: effect of corticosteroid application.
We have studied the expressions of various protein kinase C (PKC) isoenzymes in T cells and monocytes from patients with systemic lupus erythematosus (SLE), in comparison to those of healthy controls and patients with other immunological disorders. As measured by Western blotting, the levels of PKCbeta, delta, eta, epsilon, theta and zeta (but not of PKCalpha) significantly decreased in T cells of SLE patients. In monocytes, however, we observed marked suppressions only in the expressions of PKCdelta, epsilon and zeta but not in the expressions of other PKC isoforms. In vivo corticosteroid application, as well as in vitro steroid treatment of monocytes, elevated the expressions of most isoforms close to normal values; however, the decreased levels of PKCtheta and zeta were not affected by steroid application. These alterations were characteristic to SLE because we could not detect any changes in the PKC levels in mononuclear cells of primary Sjögren's syndrome and mixed connective tissue disease patients. These results suggest that impaired PKC isoenzyme pattern may exist in the T cells and monocytes of SLE patients. Furthermore, the clinically efficient glucocorticoid application in SLE can increase the expression of some members of PKC system.
Bíró
T
T
Department of Physiology and Cell Physiology Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary.
Griger
Z
Z
Kiss
E
E
Papp
H
H
Aleksza
M
M
Kovács
I
I
Zeher
M
M
Bodolay
E
E
Csépány
T
T
Szûcs
K
K
Gergely
P
P
Kovács
L
L
Szegedi
G
G
Sipka
S
S
D000305:Adrenal Cortex Hormones / Q000627:therapeutic use*; D000328:Adult; D016718:Arachidonic Acid / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology*; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008947:Mixed Connective Tissue Disease / Q000188:drug therapy / Q000201:enzymology; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000188:drug therapy / Q000201:enzymology; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology
D000305:Adrenal Cortex Hormones; D007527:Isoenzymes; D016718:Arachidonic Acid; D011493:Protein Kinase C