title: Assessment of myocardial viability in rats: Evaluation of a new method using superparamagnetic iron oxide nanoparticles and Gd-DOTA at high magnetic field.
abstract: The aim of this study was to detect salvageable peri-infarction myocardium by MRI in rats after infarction, using with a double contrast agent (CA) protocol at 7 Tesla. Intravascular superparamagnetic iron oxide (SPIO) nanoparticles and an extracellular paramagnetic CA (Gd-DOTA) were used to characterize the peri-infarction zone, which may recover function after reperfusion occurs. Infarcted areas measured from T1-weighted (T1-w) images post Gd-DOTA administration were overestimated compared to histological TTC staining (52% +/- 3% of LV surface area vs. 40% +/- 3%, P=0.03) or to T2-w images post SPIO administration (41% +/- 4%, P=0.04), whereas areas measured from T2-w images post SPIO administration were not significantly different from those measured histologically (P=0.7). Viable and nonviable myocardium portions of ischemically injured myocardium were enhanced after diffusive Gd-DOTA injection. The subsequent injection of vascular SPIO nanoparticles enables the discrimination of viable peri-infarction regions by specifically altering the signal of the still-vascularized myocardium.
authors.affiliation: INSERM U 646 Ingénierie de la Vectorisation, Université d'Angers, Angers, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Chapon
authors.forename: Laurent
authors.initials: L
authors.lastname: Lemaire
authors.forename: Florence
authors.initials: F
authors.lastname: Franconi
authors.forename: Laurent
authors.initials: L
authors.lastname: Marescaux
authors.forename: Pierre
authors.initials: P
authors.lastname: Legras
authors.forename: Benoit
authors.initials: B
authors.lastname: Denizot
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Le Jeune
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003287:Contrast Media; D006571:Heterocyclic Compounds; D007091:Image Processing, Computer-Assisted; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009203:Myocardial Infarction / Q000473:pathology*; D036103:Nanotechnology; D009942:Organometallic Compounds; D010087:Oxides; D010316:Particle Size; D010496:Pericardium / Q000473:pathology*; D051381:Rats
chemical_list: D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D010087:Oxides; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D007501:Iron