[The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood].
OBJECTIVE
To explore the role of 4-1BB molecule in the activation and proliferation of CD4(+) T cells and CD8(+) T cells and compare it with that of CD28 molecule.


METHODS
Human peripheral blood mononuclear cells (PBMCs) were stimulated in-vitro with anti-CD3 mAb. Anti-4-1BB mAb and anti-CD80 mAb were added so as to block respectively costimulatory signals of 4-1BB/4-1BBL and CD28/B7-1. Flow cytometry was used to detect the proliferation ratio of CD4(+) T cells and CD8(+) T cells and the secretion of IFN-gamma.


RESULTS
When 4-1BB/4-1BBL and CD28/B7-1 signals were blocked by anti-4-1BBL mAb and anti-CD80 mAb, the proliferative response and the secretion of IFN-gamma by CD4(+) T cells and CD8(+) T cells were obviously decreased. In the group stimulated with anti-CD3 mAb only, the ratio of CD8/CD4 T cells was 1.98+/-0.06. In the groups blocked with anti-4-1BBL mAb and anti-CD80 mAb, the ratios were 0.96+/-0.03 and 2.69+/-0.16, respectively.


CONCLUSIONS
4-1BB molecule could provide a costimulatory signal for activation and proliferation of CD4(+) T cells and CD8(+) T cells. The costimulatory signal mediated by 4-1BB molecule could mainly induce the proliferative response of CD8(+) T cells, while that mediated by CD28 molecule could induce the activation of CD4(+) T cells mainly.
Wu
Jun-ying
JY
Department of Immunology, Bengbu Medical College, Bengbu 233003, China. jywu8191@sina.com
Qian
Feng
F
Lu
He-zuo
HZ
Wang
Wei
W
Li
Bai-qing
BQ
D053330:4-1BB Ligand; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018106:CD28 Antigens / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D008024:Ligands; D048069:Tumor Necrosis Factors / Q000276:immunology*
D053330:4-1BB Ligand; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D017252:CD3 Complex; D008024:Ligands; C505974:TNFSF9 protein, human; D048069:Tumor Necrosis Factors; D007371:Interferon-gamma