Emended description of the species Lampropedia hyalina.
Three Lampropedia hyalina strains from different habitats were compared by phenotypic, chemotaxonomic and molecular characteristics. All strains form coccoid cells and have been reported to grow as square tablets of eight to 64 cells. However, two of these strains (ATCC 11041T and ATCC 43383) have apparently lost this ability, and the third strain may temporarily lose this capacity under certain cultivation conditions. The three strains showed only minor differences in metabolic characteristics: the main significant physiological difference was the ability to accumulate polyphosphate under alternating anaerobic-aerobic conditions found for DSM 15336. The three strains showed high similarity in fatty acid composition and only slight differences in the G + C content (63-67 mol%) and DNA-DNA reassociation (90-95 % relatedness). Comparative 16S rRNA gene sequence analyses on these three strains and three Lampropedia hyalina 16S rRNA gene sequences deposited at NCBI showed that they are all very similar (> 98.8 %) and that they form a distinct group among the 'Betaproteobacteria', showing between 94.6 and 93 % 16S rRNA gene similarity to members of various genera such as Acidovorax, Aquaspirillum, Brachymonas, Comamonas, Delftia and Xenophilus. Fluorescent in situ hybridization with oligonucleotide probes targeting betaproteobacteria on the 16S rRNA and 23S rRNA gene level further supported the conclusion that all investigated strains are members of the 'Betaproteobacteria'. Two oligonucleotide probes were designed and successfully applied for culture-independent identification of Lampropedia hyalina by means of fluorescent in situ hybridization.
Lee
Natuschka
N
Lehrstuhl für Mikrobiologie, TU München, Am Hochanger 4, D-85350 Freising, Germany.
Cellamare
Carmela Maria
CM
ENEA, Sezione Gestione Risorse Idriche, Via Martiri di Monte Sole, 4, I-40129 Bologna, Italy.
Bastianutti
Cristiano
C
ENEA, Sezione Gestione Risorse Idriche, Via Martiri di Monte Sole, 4, I-40129 Bologna, Italy.
Rosselló-Mora
Ramon
R
Grup d'Oceanografia Interdisciplinari Institut Mediterrani d'Estudis Avançats (CSIC-UIB), C/Miquel Marqués 21, E-07190 Esporles Mallorca, Spain.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Universität Giessen, Heinrich-Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany.
Ludwig
Wolfgang
W
Lehrstuhl für Mikrobiologie, TU München, Am Hochanger 4, D-85350 Freising, Germany.
Schleifer
Karl Heinz
KH
Lehrstuhl für Mikrobiologie, TU München, Am Hochanger 4, D-85350 Freising, Germany.
Stante
Loredana
L
ENEA, Sezione Gestione Risorse Idriche, Via Martiri di Monte Sole, 4, I-40129 Bologna, Italy.
D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000502:physiology; D042621:Comamonadaceae / Q000145:classification / Q000235:genetics; D020585:Comamonas / Q000145:classification / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D042742:Delftia / Q000145:classification / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011122:Polyphosphates / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011122:Polyphosphates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S