Heme oxygenase exerts a protective role against oxidative stress in soybean leaves.
We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for mammalian cells against oxidative stress. Here, we investigated for the first time the possible role of heme oxygenase-1 as an antioxidant defense in leaves of soybean plants. Treatment with 200 microM Cd during 48 h caused a 70% increase in thiobarbituric acid reactive substances, whereas GSH decreased 67%, guaiacol peroxidase and total superoxide dismutase also inhibited 49% and 46%, respectively. Two hundred micromolar of Cd produced the overexpression of heme oxygenase-1, as well as a 4.5-fold enhancement of its activity. Administration of biliverdin partially prevented the effects caused by Cd. Pretreatment with Zn protoporphyrin IX, a potent inhibitor of heme oxygenase, expectedly decreased heme oxygenase-1 activity to half. When the inhibitor was given before Cd, it completely prevented the enzyme induction increasing the levels of oxidative stress parameters. Collectively, these results indicated that although plant heme oxygenases share little homology to heme oxygenases from non-plant species, they also play an important protective role against oxidative cell damage.
Noriega
Guillermo O
GO
Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, Buenos Aires, Argentina.
Balestrasse
Karina B
KB
Batlle
Alcira
A
Tomaro
Maria L
ML
D000975:Antioxidants / Q000378:metabolism*; D001664:Biliverdine / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D002104:Cadmium; D006419:Heme Oxygenase (Decyclizing); D001664:Biliverdine