Simultaneous determination of thiamin and riboflavin in the sea urchin, Paracentrotus lividus, by high-performance liquid chromatography.
Thiamin and riboflavin were determined in the sea urchin, Paracentrotus lividus, by reversed-phase liquid chromatography with fluorescence detection and a mobile phase consisting of 72% 0.005 M NH(4)OAc and 28% MeOH at a flow rate of 1.450 ml/min. The total analysis time never exceeded 20 min. After acid hydrolysis and enzymatic digestion, thiamin was oxidized to thiochrome and the oxidized fraction was purified and concentrated by solid-phase extraction. Limits of detection were 9 pg/ml and 80 pg/ml for thiamin and riboflavin, respectively. Good results were obtained with respect to repeatability (RSD%<2.4) and recovery (>91.3%).
Rodríguez-Bernaldo De Quirós
A
A
Nutrition and Bromatology Department, University of Santiago de Compostela, Santiago de Compostela, La Coruña, Spain.
López-Hernández
J
J
Simal-Lozano
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012256:Riboflavin / Q000032:analysis*; D012617:Sea Urchins / Q000737:chemistry*; D013831:Thiamine / Q000032:analysis*
D012256:Riboflavin; D013831:Thiamine