Detection of zinc ions by differential circularly polarized fluorescence excitation.
A new chiroptical spectroscopic approach, differential circularly polarized fluorescence excitation (CPE), can be used to provide a selective method for detecting the presence of zinc ions. The approach utilizes the same instrumentation as fluorescence-detected circular dichroism and provides strong contrast in metal detection due to response to both chelation-enhanced fluorescence and circular dichroism upon metal ion binding. The observed contrast is therefore better than either of the parent spectroscopic detection methods. CPE also provides a strategy to reduce interference from background such as protein-based tryptophan fluorescence.
Dai
Zhaohua
Z
Department of Chemistry, New York University, New York, New York 10003, USA.
Proni
Gloria
G
Mancheno
Danny
D
Karimi
Sasan
S
Berova
Nina
N
Canary
James W
JW
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002413:Cations, Divalent; D002645:Chickens; D002942:Circular Dichroism / Q000379:methods; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry; D005454:Fluorescence Polarization / Q000379:methods*; D009113:Muramidase / Q000032:analysis / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis / Q000737:chemistry; D015032:Zinc / Q000032:analysis*
D002413:Cations, Divalent; D004527:Egg Proteins; D014364:Tryptophan; D009113:Muramidase; D015032:Zinc