Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness.
The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns.
Ge
Yue
Y
Department of Proteomics, Molecular Probes, Eugene, Oregon, USA.
Rajkumar
Lakshmanswamy
L
Guzman
Raphael C
RC
Nandi
Satyabrata
S
Patton
Wayne F
WF
Agnew
Brian J
BJ
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006031:Glycosylation; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D051381:Rats
D010750:Phosphoproteins; D011506:Proteins