title: Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China.
abstract: A novel halophilic archaeon, strain BD-1T, was isolated from Xiao-Er-Kule Lake in Xinjiang, China. The taxonomy of strain BD-1T was studied by polyphasic methods. According to 16S rRNA gene sequence similarity, strain BD-1T was phylogenetically related to Halorubrum trapanicum (98.4 %), Halorubrum sodomense (98.0 %), Halorubrum distributum (97.8 %) and Halorubrum coriense (97.3 %). Strain BD-1T is able to grow at 10 degrees C and further differs physiologically from the above species in the assimilation of sugars. The G + C content of DNA is 68.0 % (Tm). The DNA-DNA relatedness values to Hrr. trapanicum and Hrr. distributum are 47 and 24 %, respectively. It is concluded that strain BD-1T represents a novel species of the genus Halorubrum, for which the name Halorubrum xinjiangense sp. nov. is proposed. The type strain is BD-1T (= AS 1.3527T = JCM 12388T).
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Pei-Jin
authors.initials: PJ
authors.lastname: Zhou
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D001482:Base Composition; D050260:Carbohydrate Metabolism; D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006131:Growth Inhibitors / Q000494:pharmacology; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010715:Phosphatidylglycerols / Q000032:analysis; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006131:Growth Inhibitors; D010715:Phosphatidylglycerols; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D008274:Magnesium