title: [Cloning genes sensitive to mechanical stretch in osteoblasts through subtractive hybridization technique].
abstract: OBJECTIVE
  In this experiment, genes sensitive to mechanical stretch in osteoblast like cells were cloned through subtractive hybridization technique.
  
  
  METHODS
  Two dimensional mechanical stretch with deformation of 12% and frequency of 6 cycles was loaded on human osteoblastic like cell line Saos-2. Complementary deoxyribonucleic acid (cDNA) library of cells was constructed 12 h after loading, acting as tester. cDNA library of cells without loading was constructed, acting as driver. A subtractive cDNA library osteoblastic like cell stimulated with mechanical stretch was constructed through subtractive hybridization technique.
  
  
  RESULTS
  Of clones randomly selected from this library, fifteen genes were identified to be the differentially expressed genes. Comparing with the sequences published in GeneBank via Internet, two sequences located in chromosome 9 and 18 respectively were identified to be novel, which were named as stretch sensitive gene 1 and stretch sensitive gene 2.
  
  
  CONCLUSIONS
  It is an efficient approach to clone and study genes relative to mechanical stretch through subtractive hybridization technique.
authors.affiliation: Dept. of Orthodontics, Qindu Hospital, the Fourth Military Medical University, Xi'an 710032, China. cflfx@163.net
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.forename: Yin
authors.initials: Y
authors.lastname: Ding
authors.forename: Yin-zhong
authors.initials: YZ
authors.lastname: Duan
authors.forename: Zhu
authors.initials: Z
authors.lastname: Lin
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Ouyang
authors.forename: Qin
authors.initials: Q
authors.lastname: Pu
mesh_terms: D002460:Cell Line; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D009693:Nucleic Acid Hybridization; D010006:Osteoblasts / Q000502:physiology*; D013314:Stress, Mechanical