title: Dietary alpha-tocopherol affects differential gene expression in rat testes.
abstract: Gene-chip technology was employed to study the effect of dietary vitamin E (VE) on gene expression in rat testes. Male albino rats were fed with either a diet deficient in VE or a standard diet containing VE. Differential gene expression was monitored at five individual time-points over a period of 14 months with all animals individually profiled. Low VE intake resulted in the consistent up-regulation of 7-dehydrocholesterol reductase and GATA binding protein 4, both involved in testosterone synthesis. Cyclin D3, important in cell cycle progression and Wilms tumor 1, related to cancer development, were also up-regulated in the vitamin E deficient animals. This study demonstrates that low dietary VE intake has long-term effects on gene expression in the testes. Our data provides insights into the possible molecular mechanisms underlying the beneficial effects of vitamin E on the male reproductive organ.
authors.affiliation: Hugh Sinclar Human Nutrition Unit, School of Food Biosciences, University of Reading, Whiteknights, Reading, RG6 6AP, UK.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rota
authors.forename: Luca
authors.initials: L
authors.lastname: Barella
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Minihane
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Stöcklin
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D056743:Cyclin D3; D016213:Cyclins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D050991:GATA4 Transcription Factor; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; C534696:CCND3 protein, human; D002352:Carrier Proteins; C534698:Ccnd3 protein, rat; D018797:Cell Cycle Proteins; D056743:Cyclin D3; D016213:Cyclins; D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D014157:Transcription Factors; C419554:WTAP protein, human; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019995:7-dehydrocholesterol reductase; D024502:alpha-Tocopherol