Modification of polyurethaneurea with PEG and YIGSR peptide to enhance endothelialization without platelet adhesion.
Improved endothelialization without platelet adhesion is essential to enhance the long-term patency of synthetic vascular grafts and other blood-contacting devices. We have developed a dually modified polyurethaneurea by incorporating endothelial cell adhesive YIGSR peptide sequences as chain extenders and nonthrombogenic PEG as a soft segment (PUUYIGSR-PEG) in the polymer backbone. PUUYIGSR-PEG was successfully synthesized and characterized by proton NMR, FTIR, GPC, DSC, ESCA, and contact angle measurement. Despite having similar molecular weight, the peptide/PEG-modified polyurethaneurea (PUUYIGSR-PEG) showed superior mechanical properties compared to the control PEG-modified polyurethaneurea (PUUPPD-PEG). Virtually no platelet adhesion was observed on PUUYIGSR-PEG, while endothelial cell adhesion, spreading, and migration were significantly greater on PUUYIGSR-PEG compared to PUUPPD-PEG. Thus, this bioactive polymer may be an appropriate biomaterial for small diameter vascular grafts.
Jun
Ho-Wook
HW
Department of Bioengineering, Rice University, 6100 Main Street, MS 142, Houston, Texas 77005-1892, USA.
West
Jennifer L
JL
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation*; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology
D024042:Collagen Type I; D010455:Peptides; D011140:Polyurethanes; D011092:Polyethylene Glycols; C044690:polyetherurethane urea