title: Stat3 signaling is present and active during development of the central nervous system and eye of vertebrates.
abstract: Stat3, a member of the signal transducer and activator of transcription (STAT) family, plays a central role in mediating cell growth, differentiation, and survival signals. In this report, we show that Stat3 immunoreactivity was localized to specific regions in the developing mouse brain, neural tube, and eye from embryonic day 10.5 to postnatal day 0. The active form of Stat3 protein, which is phosphorylated on tyrosine 705 (pYStat3), was also found in the developing neural tube with more restricted distribution. An in ovo chick embryo electroporation assay showed that the endogenous chick Stat3 could drive consensus sis-inducible element-directed reporter gene expression. These results demonstrate that the active Stat3 protein is present and might play a role during the development of the central nervous system and eye.
authors.affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Bian
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.forename: Xinmin
authors.initials: X
authors.lastname: Cao
authors.forename: Isabelle
authors.initials: I
authors.lastname: Le Roux
authors.forename: Francois
authors.initials: F
authors.lastname: Guillemot
authors.forename: Naihe
authors.initials: N
authors.lastname: Jing
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018274:Electroporation; D004628:Embryonic Structures / Q000033:anatomy & histology / Q000502:physiology; D005123:Eye / Q000196:embryology* / Q000378:metabolism; D005260:Female; D017930:Genes, Reporter; D005865:Gestational Age; D051379:Mice; D010766:Phosphorylation; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D015534:Trans-Activators