Significance of administration of fat emulsion: hepatic changes in infant rats receiving total parenteral nutrition with and without fat.
Total parenteral nutrition (TPN) is associated with cholestasis and hepatic steatosis, which can be lethal in infants who cannot be fed orally. The present animal study focused on the metabolic complications in the liver that may occur due to the excessive administration of fat-free TPN. Thirty infant (3-week-old) male SD rats weighing 60-70 g were randomly allocated to five groups (n = 6): the OD group received an oral diet, the FT group received an oral diet and was fasted overnight on the last day of experiment before sacrifice, the 0% fat group received TPN without fat, the 20% fat group received TPN with 20% of calories from fat emulsion, and the 40% fat group received TPN with 40% of calories from fat emulsion. All TPN regimens were isocaloric, isonitrogenic, and administered for 4 days. In the 0% fat group, plasma levels of liver enzymes were significantly higher than in the other groups. Pathological examination showed hepatomegaly and severe fatty changes without cholestasis in the 0% fat group. The results of this study in infant rats indicate the importance of including fat in the TPN regimen in order to prevent the abnormal hepatic changes associated with the excessive administration of fat-free TPN.
Oshita
M
M
Department of Digestive and Pediatric Surgery, School of Medicine, University of Tokushima, 1-50-2 Kuramoto, Tokushima City, Tokushima Prefecture 770-8503, Japan. maoshita@clin.med.tokushima-u.ac.jp
Takehara
H
H
Yamaguchi
M
M
Doi
K
K
Ueda
N
N
Naito
S
S
Hiraoka
I
I
Tashiro
S
S
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009929:Organ Size; D010289:Parenteral Nutrition, Total / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase