title: Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside.
abstract: The polar extracts of Potamogeton pectinatus, P. lucens, P. perfoliatus and P. crispus (Potamogetonaceae) were analyzed by HPLC-UV-MS and their chromatographic profiles were very similar. The polar constituents of P. pectinatus were more exhaustively investigated by HPLC-UV with post-column derivatization, HPLC-MS(n) and HPLC-NMR, which allowed the on-line identification of various known flavones (dereplication). One of these compounds, luteolin 3'-O-glucoside, has never been characterized in the Potamogeton genus. The HPLC-UV-MS and HPLC-NMR analyses revealed also the presence of ent-labdane diterpene glycosides in the polar extracts of P. pectinatus and P. lucens and led to the isolation of a new ent-labdane diglycoside from P. pectinatus, beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-15,16-epoxy-12-oxo-8(17),13(16),14-ent-labdatrien-19-oate.
authors.affiliation: Laboratoire de Pharmacognosie et Phytochimie, Ecole de Pharmacie Genève-Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: Waridel
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
authors.forename: Jean-Bernard
authors.initials: JB
authors.lastname: Lachavanne
authors.forename: Kurt
authors.initials: K
authors.lastname: Hostettmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D031821:Potamogetonaceae / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004224:Diterpenes; D006027:Glycosides; D010936:Plant Extracts; C495874:ent-labdane diglycoside; C433017:labdane