[Cloning of the genes related to inhaled anesthetic action in Drosophila melanogaster].
OBJECTIVE
To search new clues to reveal the action mechanism of inhaled anesthetics.


METHODS
Three kinds of Drosophila melanogaster were used as studied models: the wild type strain (H), the sevoflurane-sensitive strain (S), and the sevoflurane-resistant strain (R). Differential display reverse transcriptional-polymerase chain reaction method was performed to examine the differentially expressed fragments between Drosophila induced with and without sevoflurane. Rapid amplification of cDNA ends (RACE) method was used to clone the full length cDNA from positive differentially expressed fragments.


RESULTS
Thirty-one differentially expressed fragments were found between the two groups. Three fragments were identified as the positive differentially expressed fragments by Northern blot analysis. Two full-length cDNAs were cloned by RACE method, among which one was a 1.0 kb Drosophila calmodulin (CaM), located on Chr.2; the other was a 4.1 kb gene with unknown function (No.45), located on Chr.3.


CONCLUSIONS
The two full-length cDNAs belong to the genes that related to anesthetic action pathway, which might participate in the regulation of cellular functions and signal transduction pathways. The two genes that we found should provide a novel way to study the mechanism of inhaled anesthetic action.
Liu
Hong
H
Division of Biochem, Fuwai Hospital, CAMS and PUMC, Beijing 100037, China.
Ren
Xiao-meng
XM
Chen
Lan-ying
LY
Liu
Jin
J
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D008738:Methyl Ethers / Q000494:pharmacology*; D000077149:Sevoflurane; D015398:Signal Transduction
D018685:Anesthetics, Inhalation; D018076:DNA, Complementary; D008738:Methyl Ethers; D000077149:Sevoflurane