title: Platelet function unaffected by ozonated autohaemotherapy in chronically haemodialysed patients.
abstract: BACKGROUND
  The therapeutic use of ozone is still a controversial medical strategy due to the potential toxicity of ozone, which is recognized as a highly reactive oxidant. The reactive oxygen species are known to induce platelet aggregation, the process involved in the development of atherosclerosis and cardiovascular events. In the present study, the influence of ozonated autohaemotherapy (O3-AHT) on the platelet function was evaluated in chronically haemodialysed patients with peripheral arterial disease.
  
  
  METHODS
  This was an oxygen-controlled, cross-over study, in which nine sessions of autohaemotherapy with oxygen administration as a control were followed by nine sessions of O3-AHT. The platelet function was assessed by the extent of spontaneous aggregation (SPA) and agonist-induced aggregation (AIPA), where different concentrations of adenosine were used as an agonist.
  
  
  RESULTS
  There were no differences between SPA and AIPA assessed after nine sessions of O3-AHT and after nine sessions of autohaemotherapy with oxygen administration. SPA and AIPA did not change after the first session of O3-AHT as compared with the levels before this procedure.
  
  
  CONCLUSIONS
  O3-AHT with ozone concentration of 50 microg/ml and citrate as an anticoagulant does not induce platelet aggregation.
authors.affiliation: Department of Nephrology Transplantology and Internal Medicine, Medical University of Gdansk, Poland. leszek.tylicki@amg.gda.pl
authors.forename: Leszek
authors.initials: L
authors.lastname: Tylicki
authors.forename: Slawomir
authors.initials: S
authors.lastname: Lizakowski
authors.forename: Bogdan
authors.initials: B
authors.lastname: Biedunkiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Skibowska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Nieweglowski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Chamienia
authors.forename: Alicja
authors.initials: A
authors.lastname: Debska-Slizien
authors.forename: Boleslaw
authors.initials: B
authors.lastname: Rutkowski
mesh_terms: D000368:Aged; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010083:Oxidants, Photochemical / Q000008:administration & dosage*; D010126:Ozone / Q000008:administration & dosage*; D010974:Platelet Aggregation / Q000187:drug effects*; D010979:Platelet Function Tests; D006435:Renal Dialysis
chemical_list: D010083:Oxidants, Photochemical; D010126:Ozone