title: Crystal structure of the clathrin adaptor protein 1 core.
abstract: The heterotetrameric adaptor proteins (AP complexes) link the outer lattice of clathrin-coated vesicles with membrane-anchored cargo molecules. We report the crystal structure of the core of the AP-1 complex, which functions in the trans-Golgi network (TGN). Packing of complexes in the crystal generates an exceptionally long (1,135-A) unit-cell axis, but the 6-fold noncrystallographic redundancy yields an excellent map at 4-A resolution. The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. Its molecular architecture closely resembles that of the core of AP-2, the plasma-membrane-specific adaptor, for which a structure has been determined. Both structures represent an "inactive" conformation with respect to binding of cargo with a tyrosine-based sorting signal. TGN localization of AP-1 depends on the small GTPase, Arf1, and the phosphoinositide, PI-4-P. We show that directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells and diminish PI-4-P-dependent, but not Arf1-dependent, liposome binding in vitro.
authors.affiliation: Children's Hospital, Howard Hughes Medical Institute, Department of Cell Biology, and CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Ekaterina E
authors.initials: EE
authors.lastname: Heldwein
authors.forename: Eric
authors.initials: E
authors.lastname: Macia
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Helen L
authors.initials: HL
authors.lastname: Yin
authors.forename: Tomas
authors.initials: T
authors.lastname: Kirchhausen
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harrison
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D034001:Adaptor Protein Complex Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012756:Sheep
chemical_list: D033961:Adaptor Protein Complex 1; D034001:Adaptor Protein Complex Subunits; D010716:Phosphatidylinositols; D011994:Recombinant Proteins