Effects of in vitro exposure to power frequency magnetic fields on UV-induced DNA damage of rat lymphocytes.
The mechanisms of biological effects of 50/60 Hz (power frequency) magnetic fields (MF) are still poorly understood. There are a number of studies indicating that MF affect biochemical processes in which free radicals are involved, such as the biological objects' response to ultraviolet radiation (UVA). Therefore, the present study was aimed to assess the effect of 50 Hz MFs on the oxidative deterioration of DNA in rat lymphocytes irradiated in vitro by UVA. UVA radiation (150 J/m2) was applied for 5 min for all groups and 50 Hz MF (40 microT rms) exposure was applied for some of the groups for 5 or 60 min. The level of DNA damage was assessed using the alkaline comet assay, the fluorescence microscope, and image analysis. It has been found that the 1 h exposure to MF caused an evident increase in all parameters consistent with damaged DNA. This suggest that MF affects the radical pairs generated during the oxidative or enzymatic processes of DNA repair.
Zmyślony
Marek
M
Nofer Institute of Occupational Medicine, Łódź, Poland. zmyslmar@imp.lodz.pl
Palus
Jadwiga
J
Dziubałtowska
Elzbieta
E
Politański
Piotr
P
Mamrot
Pawel
P
Rajkowska
Elzbieta
E
Kameduła
Maria
M
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D053938:DNA Fragmentation / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000528:radiation effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004781:Environmental Exposure; D005609:Free Radicals / Q000528:radiation effects; D007091:Image Processing, Computer-Assisted; D008214:Lymphocytes / Q000528:radiation effects*; D008280:Magnetics / Q000009:adverse effects*; D008297:Male; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects*
D004277:DNA, Single-Stranded; D005609:Free Radicals