[Enhanced action of a BTB/POZ domain protein on the expression of hsp90alpha gene in heat shock].
OBJECTIVE
To study the role of a BTB/POZ domain protein in the expression of hsp90alpha gene.


METHODS
The eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90alpha promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90alpha-CAT reporter gene was determined by competitive RT-PCR assay.


RESULTS
GRP markly increased the relative promoter activity of hsp90alpha-CAT reporter gene during heat shock.


CONCLUSIONS
GRP may promote the expression of hsp90alpha gene by participating in chromatin remolding.
Chen
Yong-jun
YJ
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Mo
Zhi-cheng
ZC
Zhang
Jing
J
Zhang
Ye
Y
Wu
Ning-hua
NH
Shen
Yu-fei
YF
D020816:Amino Acid Motifs; D000818:Animals; D000071877:Checkpoint Kinase 1; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D020798:Two-Hybrid System Techniques
C402243:ABTB1 protein, human; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018841:HSP90 Heat-Shock Proteins; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D004247:DNA; D000071877:Checkpoint Kinase 1; C000605877:grp protein, Drosophila