title: A comparison of two methods for measuring airway distensibility: nitrogen washout and the forced oscillation technique.
abstract: The measurement of airway stiffness is an important tool for studying airway remodelling in asthma. The relationship between airway calibre and lung volume (airway distensibility) was measured by forced oscillation technique (FOT) and compared with that measured by single-breath nitrogen washout (SBNW). In four non-asthmatic healthy subjects and three asthmatics, anatomical dead space (VDF) was measured by SBNW and respiratory system conductance (Grs) was measured by FOT at 6 Hz. During SBNW testing, 0.51 oxygen boluses were inhaled from three different lung volumes: functional residual capacity (FRC), 11 above FRC and near total lung capacity (TLC). Following inhalation of the oxygen bolus subjects exhaled to residual volume and then inhaled to TLC. During FOT, subjects breathed 0.5-1.01 tidal volumes but with gradually increasing end-expiratory lung volume until close to TLC, then returned to normal breathing before inhaling to TLC. This was also repeated but with reducing lung volume from TLC. Absolute lung volumes were measured by body plethysmography and related to volumes during FOT and SBNW by reference to TLC obtained at the end of each SBNW or FOT test manoeuvre. Distensibility was calculated as the linear regression slopes of VDF or Grs versus lung volume. Distensibility measured by VDF ranged 16-37 ml 1(-1) lung volume and by Grs it ranged 0.06-0.19 1 s(-1) cmH2O(-1) 1(-1) lung volume. Both distensibility measurements were correlated (Pearson's R2 = 0.91, p = 0.001). The SBNW and FOT are comparable methods for measuring airway distensibility and may have similar clinical usefulness. However, further studies are required to make any specific inferences about the relationship between airway distensibility by FOT and airway remodelling.
authors.affiliation: The Woolcock Institute of Medical Research, PO Box M77, Camperdown, NSW 2050, Australia. njb@woolcock.org.au
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Brown
authors.forename: C William
authors.initials: CW
authors.lastname: Thorpe
authors.forename: Bruce
authors.initials: B
authors.lastname: Thompson
authors.forename: Norbert
authors.initials: N
authors.lastname: Berend
authors.forename: Sue
authors.initials: S
authors.lastname: Downie
authors.forename: Sylvia
authors.initials: S
authors.lastname: Verbanck
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Salome
authors.forename: Gregory G
authors.initials: GG
authors.lastname: King
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000403:Airway Resistance / Q000502:physiology*; D001249:Asthma / Q000175:diagnosis*; D006801:Humans; D008875:Middle Aged; D008962:Models, Theoretical; D009584:Nitrogen / Q000493:pharmacokinetics*; D014109:Total Lung Capacity
chemical_list: D009584:Nitrogen