title: Molecular cytogenetic characterization shows higher genetic homogeneity in conventional renal cell carcinoma compared to other kidney cancers.
abstract: In this study seven primary kidney tumors out of 13 were cytogenetically characterized by comparative genomic hybridization (CGH) on the surgical specimens as well as by spectral karyotyping (SKY) analysis after short-term culturing. In two of the seven cases only a normal karyotype was identified. Non-clonal aberrations were observed in four of the seven cases. Overall numerical alterations were more frequent than structural changes. The two structural alterations identified constituted of a deletion of the short arm of chromosome 3 in a conventional renal cell carcinoma (RCC), and a ring chromosome derived from chromosome 8 in a papillary RCC. By CGH gains of copy number were revealed on chromosomes 3, 5, 7, 8q, and 20, while the losses encompassed 3p and 17p. In the papillary RCCs only gains were found. Comparison between SKY and CGH data suggests that the conventional RCCs are genetically more homogeneous than the other types of kidney cancer. In the two papillary RCCs, trisomies of chromosomes 7 and 17 were typical findings. In the transitional cell carcinoma different findings by CGH and SKY would suggest that these tumors constitute a heterogeneous population of tumor cells which could represent different steps of somatic evolution of tumors.
authors.affiliation: Department of Molecular Medicine, CMM L8:01, Karolinska Hospital, SE-171 76 Stockholm.
authors.forename: Andrei
authors.initials: A
authors.lastname: Alimov
authors.forename: Birgitta
authors.initials: B
authors.lastname: Sundelin
authors.forename: Ulf
authors.initials: U
authors.lastname: Bergerheim
authors.forename: Maxim
authors.initials: M
authors.lastname: Pavlenko
authors.forename: Pavel
authors.initials: P
authors.lastname: Pisa
authors.forename: Anders
authors.initials: A
authors.lastname: Zetterberg
authors.forename: Catharina
authors.initials: C
authors.lastname: Larsson
authors.forename: Svetlana
authors.initials: S
authors.lastname: Lagercrantz
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002295:Carcinoma, Transitional Cell / Q000235:genetics; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization