Molecular structure of human geminin.
The origin licensing repressor geminin is a unique bifunctional protein providing a molecular link between cellular proliferation, differentiation and genomic stability. Here we report the first molecular structure of human geminin, determined by EM and image processing at a resolution of 17.5 A. The geminin molecule is a tetramer formed by two dimers with monomers interacting via coiled-coil domains. The unusual structural organization of geminin provides molecular insight into its bifunctional nature.
Okorokov
Andrei L
AL
Bloomsbury Centre of Structural Biology, Birkbeck College, Malet Street, London, WC1E 7HX, UK.
Orlova
Elena V
EV
Kingsbury
Sarah R
SR
Bagneris
Claire
C
Gohlke
Ulrich
U
Williams
Gareth H
GH
Stoeber
Kai
K
D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000648:ultrastructure; D064248:Geminin; D006801:Humans; D008854:Microscopy, Electron; D008958:Models, Molecular; D011487:Protein Conformation
D018797:Cell Cycle Proteins; C577995:GMNN protein, human; D064248:Geminin