title: Agrobacterium-mediated genetic transformation of Perilla frutescens.
abstract: A reproducible plant regeneration and an Agrobacterium tumefaciens-mediated genetic transformation protocol were developed for Perilla frutescens (perilla). The largest number of adventitious shoots were induced directly without an intervening callus phase from hypocotyl explants on MS medium supplemented with 3.0 mg/l 6-benzylaminopurine (BA). The effects of preculture and extent of cocultivation were examined by assaying beta-glucuronidase (GUS) activity in explants infected with A. tumefaciens strain EHA105 harboring the plasmid pIG121-Hm. The highest number of GUS-positive explants were obtained from hypocotyl explants cocultured for 3 days with Agrobacterium without precultivation. Transgenic perilla plants were regenerated and selected on MS basal medium supplemented with 3.0 mg/l BA, 125 mg/l kanamycin, and 500 mg/l carbenicillin. The transformants were confirmed by PCR of the neomycin phosphotransferase II gene and genomic Southern hybridization analysis of the hygromycin phosphotransferase gene. The frequency of transformation from hypocotyls was about 1.4%, and the transformants showed normal growth and sexual compatibility by producing progenies.
authors.affiliation: National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, 441-707, South Korea.
authors.forename: Kyung-Hwan
authors.initials: KH
authors.lastname: Kim
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Donghern
authors.initials: D
authors.lastname: Kim
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Park
authors.forename: Jai-Youl
authors.initials: JY
authors.lastname: Lee
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Hwang
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Kim
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D031344:Perilla frutescens / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000254:growth & development; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic