Proteomic analysis revealed a strong association of a high level of alpha1-antitrypsin in gastric juice with gastric cancer.
To investigate the pathology of gastric disorders, we compared the proteomic patterns of gastric juice from patients with various gastric disorders. In healthy subjects pepsin A, pepsin B and gastric lipase were the major proteins detected by two-dimensional gel electrophoresis. These digestive enzymes were not detected in 60% of gastric cancer cases (18 out of 30 analyzed cases). Interestingly, an extraordinary amount of alpha(1)-antitrypsin was observed in these cases. In contrast to gastric cancer cases, alpha(1)-antitrypsin was detected in only 5% of patients (three out of 56) with chronic atrophic gastritis, and the detection frequency went up as the disease developed (one of four intestinal metaplasia cases, two of seven tubular adenoma cases, a single examined case of hyperplastic polyp and 60% of gastric cancer). Zymography showed that a 60 kDa protease strongly associated with alpha(1)-antitrypsin and mass spectrometric analysis revealed that the gastric alpha(1)-antitrypsin was a protease-cleaved form. Our data suggest that alpha(1)-antitrypsin and 60 kDa protease may serve as good diagnostic and prognostic markers for conditions associated with gastric cancer.
Lee
Kyunghee
K
Graduate School of Applied Life Science and Division of Life Science, College of Natural Sciences, Gyeongsang National University, Chinju, Korea.
Kye
Minsu
M
Jang
Joung Soon
JS
Lee
Ok Jae
OJ
Kim
Taehyo
T
Lim
Dongbin
D
D000595:Amino Acid Sequence; D005750:Gastric Juice / Q000201:enzymology / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013270:Stomach / Q000201:enzymology; D013274:Stomach Neoplasms / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
D010455:Peptides; D000515:alpha 1-Antitrypsin; D010447:Peptide Hydrolases