Pfcrt K76T mutation and its associations in imported Plasmodium falciparum malaria cases.
Over 3 years (1999-2002), 305 cases of falciparum malaria were diagnosed in Toulouse, France. After retrospective analysis, only 131 patients entered the study. The diagnosis of malaria was ensured by optical methods (QBC then thin smear examination), the results of which were checked from 1999 to mid-2001 by a conventional PCR method, replaced at that time by a real-time PCR using LightCycler. To detect Pfcrt K(lysine)76T(threonine) mutation, a real-time PCR assay was developed, the sensitivity of which was one mutated parasite per microliter, or 2% mutant asexual forms in a mixed population. Eighty-one patients harbored only mutant parasites, and 11 had a K76K/T76-mixed infection. The distribution of K76T mutation was significantly affected by the use of a chloroquine + proguanil (CQ + P) prophylaxis (P = 0.00037). Among 96 subjects who had no exposure to chloroquine or any history of CQ + P prophylaxis, the mean parasitemia was higher in K76-infected patients (P = 0.038), which suggested a lack of virulence in the falciparum mutant population.
Berry
Antoine
A
Service de Parasitologie, CHU Rangueil, 31059 Toulouse 09, France.
Vessière
Aurélia
A
Fabre
Richard
R
Benoit-Vical
Françoise
F
Marchou
Bruno
B
Massip
Patrice
P
Magnaval
Jean-François
JF
D000328:Adult; D019943:Amino Acid Substitution; D000818:Animals; D000962:Antimalarials / Q000097:blood / Q000627:therapeutic use; D044383:Black People; D002738:Chloroquine / Q000627:therapeutic use; D003062:Codon; D004351:Drug Resistance; D004359:Drug Therapy, Combination; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D008297:Male; D018512:Parasitemia; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics*; D015995:Prevalence; D002727:Proguanil / Q000627:therapeutic use; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D012189:Retrospective Studies; D014443:Tyrosine / Q000378:metabolism*; D044465:White People
D000962:Antimalarials; D003062:Codon; D015800:Protozoan Proteins; D014443:Tyrosine; D002738:Chloroquine; D002727:Proguanil