title: Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment.
abstract: OBJECTIVE
  To estimate ischemic tissue damage in ovarian cortex and to evaluate the effectiveness of ascorbic acid, an antioxidant, to protect ovarian tissue from apoptosis caused by ischemia.
  
  
  METHODS
  In vitro laboratory experiments.
  
  
  METHODS
  Academic research institute.
  
  
  METHODS
  Fresh and frozen/thawed cortical sections of bovine ovaries were incubated in MEM medium with or without ascorbic acid for a duration of 3, 24, and 48 hours at 37 degrees C.
  
  
  METHODS
  Oxygen consumption rates, lactate dehydrogenase concentrations, apoptosis rates determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and DNA fragmentation analysis.
  
  
  RESULTS
  The oxygen consumption rates were correlated inversely with the duration of incubation. When the rates of apoptosis in primordial follicles with or without ascorbic acid treatment were compared, there was no statistically significant difference between the two groups. However, the ascorbic acid treatment group showed significantly decreased apoptosis in ovarian cortex (stromal cells) with 24 hours of incubation.
  
  
  CONCLUSIONS
  The correlation between ischemic tissue damage and the duration of ischemia was verified. Ovarian cortex could tolerate ischemia at least for 3 hours. Ascorbic acid treatment reduced apoptosis in ovarian cortex up to 24 hours of incubation in vitro. It appeared that stromal cells were more vulnerable to ischemia compared to primordial follicles.
authors.affiliation: Eulji Life Science Institute, Eulji University School of Medicine, Seoul, South Korea. medssk@attglobal.net
authors.forename: S Samuel
authors.initials: SS
authors.lastname: Kim
authors.forename: Hyun Won
authors.initials: HW
authors.lastname: Yang
authors.forename: Hee Gyoo
authors.initials: HG
authors.lastname: Kang
authors.forename: Hang Heun
authors.initials: HH
authors.lastname: Lee
authors.forename: Hoi Chang
authors.initials: HC
authors.lastname: Lee
authors.forename: Duck Sung
authors.initials: DS
authors.lastname: Ko
authors.forename: Roger G
authors.initials: RG
authors.lastname: Gosden
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000473:pathology*; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid