title: [Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction].
abstract: OBJECTIVE
  Comparison of the positive intra-acrosomal proteins and spermagglutinating antibodies in human semen samples from various groups of patients.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Department of Gynecology and Obstetrics and Faculty Hospital, Charles University, Pilsen, Institute of Molecular Genetics, Czech Academy of Science, Prague.
  
  
  METHODS
  Monoclonal antibodies Hs-8 and Hs-14 (prepared in the Institute of Molecular Genetics, Prague) were used for detection of intra-acrosomal sperm proteins. Microscopic immunofluorescent methods detected the incidence, the character and the percentage of the spermatozoa specified by above-mentioned monoclonal antibodies. Direct mixed anti-immunoglobulin reactions test (MAR-test) for IgG, IgM, IgA, IgE was used for detection of spermagglutinating antibody. We examined 315 infertile patients from Special Consultation for Immunology of Reproduction and from the IVF programme, and sperm healthy donors (January 2002-March 2003).
  
  
  RESULTS
  Native donor's sperm cells had excellent positive intra-acrosomal proteins stained with monoclonal antibodies Hs8 and Hs14 and after thawing as well as. No spermagglutinating antibodies were found. In the group with normal sperm count and light microscopic morphology we found the presence of seminal spermagglutinating antibodies in 11% (IgG), in 14.5% (IgA), in 3.6% (IgM), in 5.2% (IgE). Significant positivity of intra-acrosomal protein stained with Hs8 monoclonal antibody was reached in 68.4%, and with Hs14 monoclonal antibody in even 81.3% of men. On the other hand, in oligoasthenospermatic patients we found significant increasing of spermagglutinating antibodies (for IgG 40.5%, for IgA 28.6%, for IgM 9.5%, for IgE 11.9%). Dominant good staining of intra-acrosomal proteins were seen only in 15.5% of men (for Hs8) and in 20.2% (for Hs14).
  
  
  CONCLUSIONS
  The quantitative detection of intra-acrosomal sperm proteins and spermagglutinating antibodies are used as important properties of human semen and serve for evaluation of acrosomal state, and male fertility together.
authors.affiliation: Gynekologicko-porodnická klinika LF UK a FN, Plzen.
authors.forename: J
authors.initials: J
authors.lastname: Pavlásek
authors.forename: J
authors.initials: J
authors.lastname: Peknicová
authors.forename: Z
authors.initials: Z
authors.lastname: Ulcová-Gallová
authors.forename: P
authors.initials: P
authors.lastname: Nováková
authors.forename: J
authors.initials: J
authors.lastname: Reischig
authors.forename: Z
authors.initials: Z
authors.lastname: Micanová
authors.forename: Z
authors.initials: Z
authors.lastname: Rokyta
mesh_terms: D000177:Acrosome / Q000737:chemistry*; D000328:Adult; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000276:immunology*; D013073:Sperm Agglutination
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D011506:Proteins
vernacular_title: Význam urcování intra-akrosomálních proteinů a protilátek proti spermiím v lidské reprodukci.