title: [Isolation of genomic DNA from Cinnamomum cassia Presl].
abstract: OBJECTIVE
  To extract high-quality DNA for RAPD and other molecular biology research from Chinese traditional medicine Cinnamomum cassia Presl..
  
  
  METHODS
  Improved CTAB method, low pH medium with high salt method (LPHS) and urea method were used for DNA extract. The yield and quality of DNA was analysed by electrophoresis, ratios of A260/A280 and PCR amplification.
  
  
  RESULTS
  The modified CTAB method and low pH medium with high salt method can produce high qualiy DNA for PCR amplification.
authors.affiliation: School of Life Science, Xiamen University, 361005.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Wenjie
authors.initials: W
authors.lastname: Yuan
mesh_terms: D002593:Cetrimonium Compounds / Q000737:chemistry; D032904:Cinnamomum aromaticum / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D018745:Genome, Plant; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014508:Urea / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D018744:DNA, Plant; D014508:Urea