Stress deprivation enhances manganese superoxide dismutase expression in the rat patellar tendon.
Manganese superoxide dismutase (Mn-SOD) is a key antioxidant enzyme that prevents reactive oxygen species (ROS) damage to biological tissues. Although Mn-SOD has been investigated for a variety of cells, little is known about its expression in the tendon, particularly in the stress-deprived condition. The present study demonstrated that Mn-SOD is excessively expressed in the cultured fibroblasts derived from the stress-deprived patellar tendon in the rat using subtractive hybridization analysis. In addition, we confirmed that the expression of Mn-SOD is up-regulated in the stress-deprived patellar tendon in vivo at both the mRNA and protein levels. These results suggest that Mn-SOD may play a role in regulating ROS and matrix degradation involving mechanical deterioration of the stress-deprived tendon tissue.
Kitamura
Nobuto
N
Department of Sports Medicine and Joint Reconstruction Surgery, Hokkaido University School of Medicine, Kita-ku, Kita-15 Nishi 7, Sapporo 060-8638, Japan.
Tohyama
Harukazu
H
Nishihira
Jun
J
Hatano
Motoko
M
Suzuki
Masaki
M
Yasuda
Kazunori
K
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D010329:Patella / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism; D013312:Stress, Physiological / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013710:Tendons / Q000201:enzymology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D017479:Receptors, Platelet-Derived Growth Factor