Crystallization and preliminary X-ray analysis of cellobiose phosphorylase from Cellvibrio gilvus.
A recombinant cellobiose phosphorylase from Cellvibrio gilvus has been prepared and crystallized by the sitting-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 1.5 M ammonium sulfate, 0.1 M MES buffer pH 7.0 and 5 mM glucose. A suitable crystal was obtained after 10 d incubation at 298 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 84.77, b = 98.31, c = 104.04 A, beta = 102.73 degrees. X-ray diffraction data to 2.1 A resolution have been collected at KEK-PF BL-5A.
Hidaka
Masafumi
M
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunko-ku, Tokyo 113-8657, Japan.
Kitaoka
Motomitsu
M
Hayashi
Kiyoshi
K
Wakagi
Takayoshi
T
Shoun
Hirofumi
H
Fushinobu
Shinya
S
D020633:Cellvibrio / Q000201:enzymology*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D005964:Glucosyltransferases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012984:Software; D013696:Temperature
D011994:Recombinant Proteins; D005964:Glucosyltransferases; C034714:cellobiose phosphorylase