title: Supplemental ascorbate or alpha-tocopherol induces cell death in Cu-deficient HL-60 cells.
abstract: Cytochrome c oxidase (CCO) is the Cu-dependent, terminal respiratory complex of the mitochondrial electron transport chain. Inhibition of CCO can promote oxidative stress by increasing mitochondrial production of reactive oxygen species (ROS). Because mitochondria have an important role in apoptosis as both a target and source for ROS, enhanced ROS production resulting from inhibition of CCO by Cu deficiency may trigger apoptosis. The present study focuses on the mitochondrial effects of N,N'-bis(2-aminoethyl)-1,3-propanedi-amine (TET), which inhibits CCO by causing cellular Cu deficiency, and the antioxidants ascorbate and alpha-tocopherol in a human promyelocytic leukemia cell line (HL-60). The following effects were observed: (i) TET reduced both cell growth and viability only in the presence of ascorbate or alpha-tocopherol; (ii) TET reduced CCO activity and increased mitochondrial ROS production as indicated by increased expression of Mn super-oxide dismutase, but the induction of Mn superoxide dismutase was not affected by ascorbate or alpha-tocopherol; (iii) TET acted independently of ascorbate or alpha-tocopherol in disrupting mitochondrial membrane potential; (iv) TET did not increase caspase-8 activity in the absence of ascorbate or alpha-tocopherol; and (v) TET did not increase transfer of cytochrome c from mitochondria to the cytosol unless alpha-tocopherol was present. These findings indicate that reduction in CCO activity by TET-induced Cu deficiency increased oxidative stress in HL-60 cells sufficiently to disrupt the electrochemical gradient of the inner mitochondrial membrane but did not trigger cell death. Also, ascorbate and alpha-tocopherol did not alleviate oxidative stress but may have become pro-oxidants, adding to the oxidant burden sufficiently to trigger cell death in TET-treated cells.
authors.affiliation: USDA, ARS, GFHNRC, P.O. Box 9034, Grand Forks, ND 58202-9034, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Raymond
authors.forename: W Thomas
authors.initials: WT
authors.lastname: Johnson
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology*; D001483:Base Sequence; D016923:Cell Death / Q000187:drug effects*; D003300:Copper / Q000378:metabolism*; D003470:Culture Media; D017931:DNA Primers; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D018384:Oxidative Stress; D011073:Polyamines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D017931:DNA Primers; D004791:Enzyme Inhibitors; D011073:Polyamines; D012333:RNA, Messenger; C064279:N,N-bis(2-aminoethyl)-1,3-propanediamine; D003300:Copper; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV; D024502:alpha-Tocopherol; D001205:Ascorbic Acid