[Immunofluorescent analysis of anterior lens capsule allotransplantation for chronic corneal ulcers].
This study uses immunofluorescent analysis to find out in histologic corneal sections a possible rejection reaction in anterior lens capsule allotransplantation for chronic corneal ulcers. The experiment included control group of rabbit corneas with repeat injury to epithelium and anterior stroma as well as surgically treated group. Fluorescein-conjugated human antibodies to IgA, IgM, IgG, complement and fibrinogen were used in the study. For detection we used method of direct immunofluorescence. Positive was only examination on fibrinogen in sections of treated group eyes 2 days after surgery. Last examination after 3 months failed to detect positivity in any of the tested markers. We preliminarily conclude that anterior lens capsule allotransplantation for chronic corneal ulcers does not result in rejection reaction and that components of humoral immunity do not participate in immune reaction.
Kozák
I
I
Ocná klinika LF UPJS, Kosice. ikozak_2000@yahoo.com
Trbolová
A
A
Rosocha
J
J
Jautová
J
J
Juhás
T
T
Ledecký
V
V
D000818:Animals; D002908:Chronic Disease; D003176:Complement C3 / Q000032:analysis; D003320:Corneal Ulcer / Q000601:surgery*; D019085:Fluorescent Antibody Technique, Direct; D007136:Immunoglobulins / Q000032:analysis; D007903:Lens Capsule, Crystalline / Q000276:immunology* / Q000637:transplantation*; D011817:Rabbits; D014184:Transplantation, Homologous
D003176:Complement C3; D007136:Immunoglobulins
Imunofluorescencná analýza allotransplantátov prednej sosovkovej kapsuly u chronických vredov rohovky.