Immunoblot findings of calcareous corpuscles binding proteins in cyst fluid of Taenia solium metacestodes.
After collecting calcareous corpuscles from plerocercoid of Spirometra mansoni (sparganum), we evaluated the antigenic values of calcareous corpuscles binding proteins obtained from the cyst fluid of Taenia solium metacestodes. Immunoblot analysis revealed that cysticercosis patient sera strongly recognized 10 and 95 kDa calcareous corpuscles binding proteins. This result demonstrated that calcareous corpuscles are bound with major secretory antigenic proteins, which is possibly involved in the secretory pathways of the 10 and 95 kDa proteins presenting in the cyst fluid of T. solium metacestodes.
Yang
Hyun-Jong
HJ
Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-710, Repubic of Korea. parayang@ewha.ac.kr
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002352:Carrier Proteins / Q000276:immunology*; D003551:Cysticercosis / Q000175:diagnosis / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008970:Molecular Weight; D012698:Serologic Tests; D013032:Sparganum; D041201:Taenia solium / Q000737:chemistry* / Q000276:immunology
D000947:Antigens, Helminth; D002352:Carrier Proteins; D015801:Helminth Proteins