Biosynthetic pathway for the C45 polyprenol, solanesol, in tobacco.
Feeding experiments were independently performed with [1-13C]deoxy-D-xylulose triacetate and (RS)-[2-13C]mevalonolactone in the tobacco plant. The labeling pattern for solanesol was elucidated to reveal that the isoprene moiety of solanesol would be derived from deoxy-xylulose. The result strongly suggests that tobacco solanesol is biosynthesized via the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway.
Fukusaki
Ei'ichiro
E
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Japan. fukusaki@bio.eng.osaka-u.ac.jp
Takeno
Shinya
S
Bamba
Takeshi
T
Okumoto
Hiroshi
H
Katto
Hiroko
H
Kajiyama
Shin'ichiro
S
Kobayashi
Akio
A
D002070:Butadienes / Q000378:metabolism; D002250:Carbon Radioisotopes; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D045782:Hemiterpenes / Q000378:metabolism; D010420:Pentanes / Q000378:metabolism; D011849:Radioactive Tracers; D013403:Sugar Phosphates / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism*; D014996:Xylulose / Q000031:analogs & derivatives* / Q000378:metabolism
C114232:2-C-methylerythritol 4-phosphate; D002070:Butadienes; D002250:Carbon Radioisotopes; D045782:Hemiterpenes; D010420:Pentanes; D011849:Radioactive Tracers; D013403:Sugar Phosphates; D013729:Terpenes; C005059:isoprene; D014996:Xylulose; C029724:1-deoxy-2-pentulose; C017719:solanesol; D004896:Erythritol