Binding of pro-matrix metalloproteinase 9 by Fusobacterium nucleatum subsp. nucleatum as a mechanism to promote the invasion of a reconstituted basement membrane.
In this study, we investigated the ability of Fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (MMP-9) activity. Binding of pro-MMP-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. Zymography and a colorimetric assay showed that bound pro-MMP-9 can be converted into a proteolytically active form. The potential contribution of this acquired host activity in tissue invasion was demonstrated using a reconstituted basement membrane (Matrigel).
Gendron
Renée
R
Groupe de Recherche En Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada.
Plamondon
Pascale
P
Grenier
Daniel
D
D001485:Basement Membrane / Q000378:metabolism* / Q000382:microbiology*; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism*; D003124:Colorimetry; D004338:Drug Combinations; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D016967:Fusobacterium nucleatum / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism*
D004338:Drug Combinations; D004792:Enzyme Precursors; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D017364:Collagenases; C120487:pro-matrix metalloproteinase 9; D020780:Matrix Metalloproteinase 9