Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene.
Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.
Spitalska
Eva
E
School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
Torina
Alessandra
A
Cannella
Vincenza
V
Caracappa
Santo
S
Sparagano
Olivier A E
OA
D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012757:Sheep Diseases / Q000469:parasitology; D013045:Species Specificity; D016794:Theileria / Q000145:classification* / Q000235:genetics; D016796:Theileria annulata / Q000145:classification* / Q000235:genetics; D013801:Theileriasis / Q000469:parasitology*; D013987:Ticks / Q000469:parasitology*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D019003:Deoxyribonuclease HpaII