title: Lipid raft proteins and their identification in T lymphocytes.
abstract: This review focuses on how membrane lipid rafts have been detected and isolated, mostly from lymphocytes, and their associated proteins identified. These proteins include transmembrane antigens/receptors, GPI-anchored proteins, cytoskeletal proteins, Src-family protein kinases, G-proteins, and other proteins involved in signal transduction. To further understand the biology of lipid rafts, new methodological approaches are needed to help characterize the raft protein component, and changes that occur in this component as a result of cell perturbation. We describe the application of new proteomic approaches to the identification and quantification of raft proteins in T-lymphocytes. Similar approaches, applied to other model cell systems, will provide valuable new insights into both cellular signal transduction and lipid raft biology.
authors.affiliation: Institute for Systems Biology, Seattle, WA 98103, USA.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
authors.forename: Priska D
authors.initials: PD
authors.lastname: von Haller
authors.forename: Eugene
authors.initials: E
authors.lastname: Yi
authors.forename: Samuel
authors.initials: S
authors.lastname: Donohoe
authors.forename: Kelly
authors.initials: K
authors.lastname: Vaughn
authors.forename: Andrew
authors.initials: A
authors.lastname: Keller
authors.forename: Alexey I
authors.initials: AI
authors.lastname: Nesvizhskii
authors.forename: Jimmy
authors.initials: J
authors.lastname: Eng
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Li
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Julian D
authors.initials: JD
authors.lastname: Watts
mesh_terms: D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology; D017261:Glycosylphosphatidylinositols / Q000502:physiology; D006801:Humans; D021962:Membrane Microdomains / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D020543:Proteome; D013601:T-Lymphocytes / Q000648:ultrastructure*
chemical_list: D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D020543:Proteome; D011505:Protein-Tyrosine Kinases; D019204:GTP-Binding Proteins