Analysis of hematopoietic progenitor cells in patients with myelodysplastic syndromes according to their cytogenetic abnormalities.
The present work analyzes the hematopoietic progenitor cells (HPC) in myelodysplastic syndrome (MDS) patients using both an immunophenotypical and a functional approaches in order to know whether they are similar in patients with or without cytogenetic abnormalities. Among CD34+ HPC, the proportion of myeloid committed progenitors was higher in patients with an abnormal karyotype. Ninety MDS patients were studied. Patients with abnormal karyotype showed a similar platting efficiency than patients with normal cytogenetics. Trisomy 8 and 5q- showed a significant higher P.E. than patients with normal karyotype or monosomy 7. We observed that when the most immature HPC were studied, the total number of granulo-monocytic colonies produced by LTBMC was higher in the normal karyotype group. In summary, the present study shows that in MDS the HPC are impaired; this impairment is deeper in patients with abnormal karyotype.
López-Holgado
Natalia
N
Department of Hematology, Hospital Clínico Universitario de Salamanca, Paseo de San Vicente 58-182, 37007, Spain.
Arroyo
Jose Luis
JL
Pata
Carmen
C
Villarón
Eva
E
Sánchez Guijo
Fermín
F
Martín
Alejandro
A
Hernández Rivas
Jesús María
JM
Orfao
Alberto
A
San Miguel
Jesús Fernando
JF
Del Cañizo Fernández-Roldán
M Consuelo
MC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D014314:Trisomy