Optimizing electroporation conditions for intracellular delivery of morpholino antisense oligonucleotides directed against the hepatitis C virus internal ribosome entry site.
Jubin
Ronald
R
Department of Antiviral Therapy, Schering Plough Research Institute, Kenilworth, NJ, USA.
D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018274:Electroporation / Q000379:methods*; D005821:Genetic Techniques; D006367:HeLa Cells; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010957:Plasmids / Q000378:metabolism; D012367:RNA, Viral; D012270:Ribosomes / Q000648:ultrastructure*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
D004396:Coloring Agents; D016376:Oligonucleotides, Antisense; D012367:RNA, Viral; D013778:Tetrazolium Salts; D013844:Thiazoles; D008156:Luciferases; C022616:thiazolyl blue