Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors signaling complexes in Bergmann glia.
Glutamate, the major excitatory neurotransmitter, induces a wide array of signals from the membrane to the nucleus regulating gene expression. In Bergmann glia, Ca2+ -permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazole- propionic acid (AMPA) receptors are involved in the short- and long-term interactions between these cells and the neurons that they surround. After activation, AMPA receptors become tyrosine phosphorylated and by these means form multiprotein signaling complexes. To characterize these events, cultured chick Bergmann glia cells as well as chick cerebellar slices were exposed to glutamate, and, by using a combination of immunoprecipitation assays coupled to Western blot analysis, we identified several signaling proteins that become associated with these receptors. A dose- and time-dependent association among AMPA receptors, the focal adhesion kinase pp125FAK, the phosphatidylinositol-3 kinase and paxillin was found. These results extend the concept of the transducisome to AMPA receptors and provide a framework in which a plausible control of the cytoskeletal network by glutamate is taking place, most possibly through AMPA receptors.
Millán
Alejandro
A
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México DF, México.
Arias-Montaño
José Antonio
JA
Méndez
J Alfredo
JA
Hernández-Kelly
L Clara R
LC
Ortega
Arturo
A
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002642:Chick Embryo; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D018091:Receptors, AMPA / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
D018091:Receptors, AMPA; D018698:Glutamic Acid