title: [A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].
abstract: OBJECTIVE
  To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.
  
  
  METHODS
  Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.
  
  
  RESULTS
  The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.
  
  
  CONCLUSIONS
  The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing 100101, China.
authors.forename: Yong-ning
authors.initials: YN
authors.lastname: Wang
authors.forename: Rong-xiu
authors.initials: RX
authors.lastname: Li
authors.forename: Cheng-wu
authors.initials: CW
authors.lastname: Li
authors.forename: Yun-hua
authors.initials: YH
authors.lastname: Gao
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Guo
authors.forename: Zhong-ze
authors.initials: ZZ
authors.lastname: Gu
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives; D006801:Humans; D006961:Hyperparathyroidism / Q000097:blood; D007011:Hypoparathyroidism / Q000097:blood; D007118:Immunoassay / Q000379:methods; D049449:Luminescence; D013972:Thyrotropin / Q000097:blood*
chemical_list: C071872:3-(2'-(spiro-5-chloroadamantane))-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; D013972:Thyrotropin; D000218:Adamantane