title: Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.
abstract: The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.
authors.affiliation: Department of Laboratory Medicine and Pathology, Box 609 Mayo Building, 420 Delaware Street, S.E., University of Minnesota, Minneapolis 55455, USA.
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Henry-Stanley
authors.forename: Robb M
authors.initials: RM
authors.lastname: Garni
authors.forename: Carol L
authors.initials: CL
authors.lastname: Wells
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry* / Q000378:metabolism; D018938:Caco-2 Cells; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D020895:Enterocytes / Q000382:microbiology*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D011806:Quinolinium Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C007061:C.I. Fluorescent Brightening Agent 28