title: Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis.
abstract: BACKGROUND
  The umbilical cord epithelium (UCE) is composed of a single epithelial layer covering mucous connective tissue and it is thought to derive from the amniotic epithelium. Interestingly, UCE cells express not only simple and mucous epithelial keratins (CK8 and CK4/CK13), but also stratified epithelial keratins (CK1/10) and cornified cell envelope (CCE)-associated proteins.
  
  
  OBJECTIVE
  To understand the nature of UCE, UCE cells were cultured under the same conditions of organotypic culture of epidermal keratinocytes and grafted onto the back of nude mice.
  
  
  METHODS
  UCE cells isolated from fresh umbilical cord specimens were cultured using serum-free keratinocyte growth medium, and plated on a fibroblast-populated collagen matrix using air-liquid interface methods. UCE cells were transplanted onto the back of Balb C nu/nu mice as a thin epithelial sheet grown on a collagen matrix.
  
  
  RESULTS
  UCE cells formed a multi-layered stratified epithelium both in organotypic culture and surface transplantation. Regarding the expression profile of differentiation-specific proteins, such as keratins, the CCE-precursor proteins and junctional proteins, the reconstructed epithelium showed a close similarity to natural epidermis in organotypic culture.
  
  
  CONCLUSIONS
  These results suggest the possibility that UCE cells can differentiate and organize into an epidermis-like structure, when exposed to the appropriate conditions which is similar to those of cutaneous epidermis.
authors.affiliation: Department of Dermatology, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan. mizog@med.juntendo.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mizoguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Suga
authors.forename: Borisut
authors.initials: B
authors.lastname: Sanmano
authors.forename: Shigaku
authors.initials: S
authors.lastname: Ikeda
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D015415:Biomarkers; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009924:Organ Culture Techniques / Q000379:methods*; D016038:Skin Transplantation; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D007633:Keratins