Osteopontin expression in particle-induced lung disease.
Osteopontin (OPN) is a secreted cytokine with cell adhesive and chemoattractive functions whose expression is induced by a variety of environmental toxicants. It has been implicated in the pathogenesis of several pulmonary granulomatous and fibrotic conditions. For these reasons the authors investigated OPN expression in experimental particle-induced lung disease using a titanium dioxide exposure model in the rat. Under exposure conditions that resulted in fibroproliferative lung disease, rats had significant increases in total lung OPN mRNA expression and increased levels of OPN protein in bronchoalveolar lavage fluid (BALF) prior to the development of lesions. OPN immunoreactivity studies of lesion development provide evidence that this multifunctional cytokine may be important in the pathogenesis of particle-induced lung disease. Findings suggest that OPN may serve as an important biomarker for particle-induced lung disease.
Mangum
James
J
CIIT Centers for Health Research, Research Triangle Park, North Carolina 27709-2137, USA. jmangum@ciit.org
Bermudez
Edilberto
E
Sar
Madhabananda
M
Everitt
Jeffrey
J
D000818:Animals; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D004781:Environmental Exposure; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology / Q000502:physiology*; D053495:Osteopontin; D011658:Pulmonary Fibrosis / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011916:Rats, Inbred F344; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms; D014025:Titanium
D015415:Biomarkers; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; C009495:titanium dioxide; D014025:Titanium