Cyst morphology and sequence analysis of the small subunit rDNA and ef1 alpha identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia.
Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1 alpha) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1 alpha were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1 alpha sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.
Adams
Peter J
PJ
Division of Veterinary and Biomedical Sciences, WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Murdoch University, Murdoch, Western Australia 6150, Australia.
Monis
Paul T
PT
Elliot
Aileen D
AD
Thompson
R C Andrew
RC
D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005838:Genotype; D005872:Giardia / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary; D006790:Host-Parasite Interactions; D008394:Marsupialia / Q000469:parasitology*; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D014904:Western Australia / Q000453:epidemiology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D020648:Peptide Elongation Factor 1