The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential.
The genome of Bacillus licheniformis DSM13 consists of a single chromosome that has a size of 4,222,748 base pairs. The average G+C ratio is 46.2%. 4,286 open reading frames, 72 tRNA genes, 7 rRNA operons and 20 transposase genes were identified. The genome shows a marked co-linearity with Bacillus subtilis but contains defined inserted regions that can be identified at the sequence as well as at the functional level. B. licheniformis DSM13 has a well-conserved secretory system, no polyketide biosynthesis, but is able to form the lipopeptide lichenysin. From the further analysis of the genome sequence, we identified conserved regulatory DNA motives, the occurrence of the glyoxylate bypass and the presence of anaerobic ribonucleotide reductase explaining that B. licheniformis is able to grow on acetate and 2,3-butanediol as well as anaerobically on glucose. Many new genes of potential interest for biotechnological applications were found in B. licheniformis; candidates include proteases, pectate lyases, lipases and various polysaccharide degrading enzymes.
Veith
Birgit
B
Göttingen Genomics Laboratory and Competence Centre for Genome Research on Bacteria, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany.
Herzberg
Christina
C
Steckel
Silke
S
Feesche
Jörg
J
Maurer
Karl Heinz
KH
Ehrenreich
Petra
P
Bäumer
Sebastian
S
Henne
Anke
A
Liesegang
Heiko
H
Merkl
Rainer
R
Ehrenreich
Armin
A
Gottschalk
Gerhard
G
D001407:Bacillus / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics; D001482:Base Composition; D001692:Biological Transport / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry*; D010450:Endopeptidases / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D020459:Genes, rRNA; D016680:Genome, Bacterial; D023281:Genomics; D006038:Glyoxylates / Q000378:metabolism; D008049:Lipase / Q000235:genetics; D008074:Lipoproteins / Q000235:genetics; D008660:Metabolism / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010456:Peptides, Cyclic / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012264:Ribonucleotide Reductases / Q000235:genetics; D017422:Sequence Analysis, DNA; D026801:Synteny; D019895:Transposases / Q000235:genetics
D004269:DNA, Bacterial; D006038:Glyoxylates; D008074:Lipoproteins; D010456:Peptides, Cyclic; C094325:lichenysin A; D012343:RNA, Transfer; D012264:Ribonucleotide Reductases; C101141:anaerobic ribonucleotide reductase; D019895:Transposases; D008049:Lipase; D010450:Endopeptidases; D011133:Polysaccharide-Lyases; C023911:pectate lyase; C031150:glyoxylic acid