Suppression of artifacts induced by homonuclear decoupling in amino-acid-type edited methyl 1H-13C correlation experiments.
A detailed theoretical and experimental analysis of the artifacts induced by homonuclear band-selective decoupling during CT frequency labeling is presented. The effects are discussed in the context of an amino-acid-type editing filter implemented in (1)H-(13)C CT-HSQC experiments of methyl groups in proteins. It is shown that both Bloch-Siegert shifts and modulation sidebands are efficiently suppressed by using additional off-resonance decoupling as proposed by Zhang and Gorenstein [J. Magn. Reson. 132 (1998) 81], and appropriate adjustment of a set of pulse sequence parameters. The theoretical predictions are confirmed by experiments performed on (13)C-labeled protein samples, yielding artifact-free amino-acid-type edited methyl spectra.
Van Melckebeke
Hélène
H
Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A.-UJF 41, rue Jules Horowitz, 38027 Grenoble Cedex, France.
Simorre
Jean-Pierre
JP
Brutscher
Bernhard
B
D000596:Amino Acids / Q000737:chemistry*; D016477:Artifacts; D002147:Calmodulin / Q000737:chemistry*; D002247:Carbon Isotopes; D006860:Hydrogen Bonding; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D000596:Amino Acids; D002147:Calmodulin; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes