Development of a gene-based radiation hybrid map of chicken Chromosome 7 and comparison to human and mouse.
To validate the ChickRH6 whole-genome radiation hybrid (WGRH) panel, we constructed a map of chicken Chromosome 7 based on 19 microsatellite markers from the genetic map and 76 ESTs (expressed sequence tags), whose efficient targeted development was made possible by using the ICCARE software. This high-density radiation hybrid (RH) map of a chicken macrochromosome gives us indications on characteristics of ChickRH6. The potential resolution of the panel is 325 kb and the practical resolution of our framework map is 1.3 Mb. Based on these results, a complete framework map of the chicken genome would comprise 1000 markers. The marker order is in good agreement with the genetic map and comparison with the human and mouse sequence maps revealed a number of internal rearrangements.
Morisson
Mireille
M
Laboratoire de Génétique Cellulaire, INRA, Chemin de Borde Rouge, 31326, Castanet-Tolosan, France. mmorisso@toulouse.inra.fr
Jiguet-Jiglaire
Carine
C
Leroux
Sophie
S
Faraut
Thomas
T
Bardes
Suzanne
S
Feve
Katia
K
Genet
Carine
C
Pitel
Frédérique
F
Milan
Denis
D
Vignal
Alain
A
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021101:Radiation Hybrid Mapping / Q000662:veterinary*
D005819:Genetic Markers; D004247:DNA