title: A soluble flavonoid-glycoside, alphaG-rutin, is absorbed as glycosides in the isolated gastric and intestinal mucosa.
abstract: We investigated the absorption and metabolism of the highly soluble quercetin glycoside alphaG-rutin, a glucose adduct of insoluble rutin, using the isolated mucosa of the rat stomach and intestines equipped with the Ussing chamber. alphaG-rutin and rutin appeared in the serosal sides of the gastric body and all the intestinal mucosa after the addition of alphaG-rutin (1 mM) to the mucosal fluid. The degree of alphaG-rutin appearance was much lower in the gastric fundus than in the other parts. Quercetin was not found in the mucosal fluid of any mucosal specimen. The concentrations (microM) of alphaG-rutin and rutin in the serosal fluid as a result of transport from the mucosal side increased time-dependently and linearly with mucosal alphaG-rutin concentration (1, 10 or 100 mM). The highest transport was shown in the ileal mucosa. These results indicate that alphaG-rutin is partly hydrolyzed to rutin through the intestine and absorbed as such.
authors.affiliation: Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsukawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Mineo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Chiji
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
mesh_terms: D000818:Animals; D001692:Biological Transport; D005419:Flavonoids / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D012995:Solubility; D014312:Trisaccharides / Q000032:analysis / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D006027:Glycosides; D014312:Trisaccharides; C069037:4(G)-alpha-glucopyranosyl-rutin; D012431:Rutin