Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.
A duplex real-time PCR assay was developed for the assignment of Mycobacterium tuberculosis isolates to the three genotypic groups based on the katG463/gyrA95 polymorphism. The assay was as sensitive and specific as nucleotide sequencing and proved also able to detect unambiguously the isolate genotype in clinical specimens.
Rindi
Laura
L
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35-39, Università di Pisa, I-56127 Pisa, Italy. rindi@biomed.unipi.it
Lari
Nicoletta
N
Bonanni
Daniela
D
Garzelli
Carlo
C
D001426:Bacterial Proteins / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014376:Tuberculosis / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D027081:DNA Gyrase