Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment.
A Gram-negative, yellow-pigmented, halophilic bacterial strain US6-1T, which degrades high-molecular-mass polycyclic aromatic hydrocarbons of two to five rings, was isolated from muddy sediment of Ulsan Bay, Republic of Korea. The 16S rRNA gene of the isolate showed high sequence similarity to Novosphingobium subarcticum (96.23 %) and Sphingopyxis alaskensis (96.18 %); however, the isolate formed a distinct phyletic line within the genus Novosphingobium. DNA-DNA relatedness between US6-1T and the closest strain N. subarcticum revealed that strain US6-1T was independent from this species. Isolate US6-1T had ubiquinone 10 and a DNA G + C ratio of 61.1 mol%. Major fatty acids were octadecanoic acid (18 : 1omega7), hexadecanoic acid (16 : 1omega7) and 2-hydroxy-myristic acid (14 : 0 2-OH). On the basis of polyphasic taxonomic evidence, strain US6-1T is proposed to represent a novel species in the genus Novosphingobium for which the name Novosphingobium pentaromativorans sp. nov. is proposed. The type strain is US6-1T (= KCTC 10454T = JCM 12182T).
Sohn
Jae Hak
JH
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, PO Box 29, 425-600, Republic of Korea.
Kwon
Kae Kyoung
KK
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, PO Box 29, 425-600, Republic of Korea.
Kang
Ji-Hyun
JH
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, PO Box 29, 425-600, Republic of Korea.
Jung
Hong-Bae
HB
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, PO Box 29, 425-600, Republic of Korea.
Kim
Sang-Jin
SJ
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan, PO Box 29, 425-600, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007723:Korea; D008969:Molecular Sequence Data; D009227:Myristic Acids / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013229:Stearic Acids / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009227:Myristic Acids; D011084:Polycyclic Aromatic Hydrocarbons; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013229:Stearic Acids; C017418:alpha-hydroxymyristic acid; D019308:Palmitic Acid; C031183:stearic acid