A new method of orotracheal intubation in mice.
A new method of orotracheal intubation in mice is described. After intraperitoneal induction of anaesthesia, 36 male animals, belonging to common laboratory strains, have been intubated with the aid of a straight, small bore arthroscope, connected to a video-camera. After the insertion of a guide wire of appropriate size across the vocal cords, a polyethylene (PE) cannula has been introduced over it as an endotracheal tube. Success rate has been 100% both in first intubations and in re-intubations; all procedures have been performed in a mean time of about 3 min. Post-mortem examination of mice did not show any significant damage to upper airway mucosae related to the technique.
Vergari
A
A
Istituto di Anestesiologia e Rianimazione, Facoltà di Medicina e Chirurgia, Università Cattolica del Sacro Cuore - Rome, (Italy).
Gunnella
B
B
Rodolà
F
F
Frassanito
L
L
Musumeci
M
M
Palazzesi
S
S
Casalinuovo
I A
IA
D000818:Animals; D000830:Animals, Laboratory; D019723:Endoscopes / Q000639:trends; D004867:Equipment Design; D007442:Intubation, Intratracheal / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D007558:Italy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013997:Time Factors; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods