Engineering surfaces for bioconjugation: developing strategies and quantifying the extent of the reactions.
This study presents two-step and multistep reactions for modifying the surface of plasma-functionalized poly(tetrafluoroethylene) (PTFE) surfaces for subsequent conjugation of biologically relevant molecules. First, PTFE films were treated by a radiofrequency glow discharge (RFGD) ammonia plasma to introduce amino groups on the fluoropolymer surface. This plasma treatment is well optimized and allows the incorporation of a relative surface concentration of approximately 2-3.5% of amino groups, as assessed by chemical derivatization followed by X-ray photoelectron spectroscopy (XPS). In a second step, these amino groups were further reacted with various chemical reagents to provide the surface with chemical functionalities such as maleimides, carboxylic acids, acetals, aldehydes, and thiols, that could be used later on to conjugate a wide variety of biologically relevant molecules such as proteins, DNA, drugs, etc. In the present study, glutaric and cis-aconitic anhydrides were evaluated for their capability to provide carboxylic functions to the PTFE plasma-treated surface. Bromoacetaldehyde diethylacetal was reacted with the aminated PTFE surface, providing a diethylacetal function, which is a latent form of aldehyde functionality. Reactions with cross-linkers such as sulfo-succinimidyl derivatives (sulfo-SMCC, sulfo-SMPB) were evaluated to provide a highly reactive maleimide function suitable for further chemical reactions with thiolated molecules. Traut reagent (2-iminothiolane) was also conjugated to introduce a thiol group onto the fluoropolymer surface. PTFE-modified surfaces were analyzed by XPS with a particular attention to quantify the extent of the reactions that occurred on the polymer. Finally, surface immobilization of fibronectin performed using either glutaric anhydride or sulfo-SMPB activators demonstrated the importance of selecting the appropriate conjugation strategy to retain the protein biological activity.
Gauvreau
Virginie
V
Unité de Biotechnologie et de Bioingénierie, Centre de Recherche de l'Hôpital Saint-François d'Assise, C.H.U.Q., 10 rue de l'Espinay, Québec, Québec, Canada, G1L 3L5.
Chevallier
Pascale
P
Vallières
Karine
K
Petitclerc
Eric
E
Gaudreault
René C
RC
Laroche
Gaétan
G
D001672:Biocompatible Materials / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002616:Chemical Engineering / Q000379:methods*; D006801:Humans; D010949:Plasma / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials