title: Extracellular recordings from patterned neuronal networks using planar microelectrode arrays.
abstract: Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.
authors.affiliation: Cornell University, Ithaca, NY 14850 USA. cdjame@sandia.gov
authors.forename: Conrad D
authors.initials: CD
authors.lastname: James
authors.forename: Andrew J H
authors.initials: AJ
authors.lastname: Spence
authors.forename: Natalie M
authors.initials: NM
authors.lastname: Dowell-Mesfin
authors.forename: Rifat J
authors.initials: RJ
authors.lastname: Hussain
authors.forename: Karen L
authors.initials: KL
authors.lastname: Smith
authors.forename: Harold G
authors.initials: HG
authors.lastname: Craighead
authors.forename: Michael S
authors.initials: MS
authors.lastname: Isaacson
authors.forename: William
authors.initials: W
authors.lastname: Shain
authors.forename: James N
authors.initials: JN
authors.lastname: Turner
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005110:Extracellular Space / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000502:physiology; D008839:Microelectrodes; D009415:Nerve Net / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001672:Biocompatible Materials