Amplification of evoked potentials recorded from mouse hippocampal slices by very low repetition rate pulsed magnetic fields.
The influence of low repetition rate pulsed magnetic fields (LRMF) on the evoked potential (population spike) recorded from mouse hippocampal slices was investigated. LRMF were applied according to two protocols. In protocol A, LRMF applied with a constant strength (15 mT) and frequency ranging from 0.03 to 0.5 Hz resulted in an amplification of the potential. Although the frequency of 0.16 Hz was the most effective, enhancing the population spike by over 280%, it also caused an increase in spontaneous activity, seizures, and cessation of neuronal activity in 50% of the slices. In protocol B, LRMF were applied with a variable intensity (9-15 mT) and in cycles of different duration ranging from 5 to 20 min. While an increase in the amplitude of the population spike was observed in all slices exposed to LRMF applied according to protocol B, the longest exposure was the most effective. Neither seizures nor an increase in the spontaneous activity were observed in this group of the slices. These results support and extend our previous data and characterize further the relation between the pattern of applied magnetic fields and their influence on the nervous system.
Wieraszko
Andrzej
A
Department of Biology/Program in Neuroscience, The College of Staten Island/CUNY, Staten Island, NY 10314, USA. Wieraszko@Postbox.CSI.CUNY.Edu
D000818:Animals; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D005071:Evoked Potentials / Q000528:radiation effects*; D006624:Hippocampus / Q000503:physiopathology / Q000528:radiation effects*; D008280:Magnetics; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000528:radiation effects; D011829:Radiation Dosage; D012640:Seizures / Q000503:physiopathology; D013997:Time Factors