title: The serum proteome of Equus caballus.
abstract: We constructed a reference two-dimensional protein map for horse (Equus caballus) serum. The serum proteins were separated by two-dimensional electrophoresis (2-DE); 29 different gene products were identified. Proteins represented by 25 spots/spot groups were identified by tandem nanoelectrospray mass spectrometry (MS), four by matrix-assisted laser desorption ionization time-of-flight (TOF) MS and one was sequenced by TOF-TOF technology. The identities of four proteins were deduced by similarity to the human plasma protein database. In selected cases, i.e. the immunoglobulins, immunoblotting with specific antibodies provided additional information about the respective proteins. Albumin was detected as the full-length protein and as fragments of various sizes. Spots representing products of different mass and charge were also detected for alpha1-antitrypsin, haptoglobin and transthyretin. Thus, despite the fact that the Equus caballus genome is incompletely characterized, we were able to identify almost all moderate to high abundance proteins stained in the serum 2-DE pattern.
authors.affiliation: Institute of Medical Chemistry, Department of Natural Sciences, University of Veterinary Medicine Vienna, Vienna, Austria. ingrid.miller@vu-wien.ac.at
authors.forename: Ingrid
authors.initials: I
authors.lastname: Miller
authors.forename: Arno
authors.initials: A
authors.lastname: Friedlein
authors.forename: George
authors.initials: G
authors.lastname: Tsangaris
authors.forename: Antony
authors.initials: A
authors.lastname: Maris
authors.forename: Michael
authors.initials: M
authors.lastname: Fountoulakis
authors.forename: Manfred
authors.initials: M
authors.lastname: Gemeiner
mesh_terms: D000418:Albumins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006736:Horses / Q000097:blood*; D007136:Immunoglobulins / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000418:Albumins; D007136:Immunoglobulins; D010455:Peptides; D011506:Proteins; D020543:Proteome