Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non-Hodgkin's lymphoma.
DNA sequences from simian virus 40 (SV40) have been detected in various human tumors, including non-Hodgkin's lymphomas (NHLs), by highly sensitive PCR techniques. However, there is a strong debate as to whether SV40 is present in lymphoma cells. Using immunohistochemistry and tissue microarrays, we investigated a series of French and Canadian cases of Hodgkin's lymphomas (HLs) and NHLs and tried to detect the SV40 large T antigen using routine paraffin sections with standard and highly sensitive catalyzed system amplification methods. None of the cases of HLs (n = 250) or NHLs (n = 232) were found to contain a single large T antigen-positive cell, whereas 2 positive controls were repeatedly stained. Therefore, our results do not support the hypothesis that SV40 is implicated in the etiology of human lymphomas.
Brousset
Pierre
P
Department of Pathology, Purpan Hospital and INSERM U563 (CPTP), CHU Purpan, Toulouse, France. brousset.p@chu-toulouse.fr
de Araujo
Valquiria
V
Gascoyne
Randy D
RD
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002170:Canada / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005602:France / Q000453:epidemiology; D006689:Hodgkin Disease / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008228:Lymphoma, Non-Hodgkin / Q000209:etiology / Q000378:metabolism*; D008875:Middle Aged; D013539:Simian virus 40 / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming