title: Detailed physicochemical characterization of the 2S storage protein from rape (Brassica napus L.).
abstract: Chromatographic, chemical, and spectroscopic techniques were used to characterize the physicochemical properties of napin purified by preparative chromatography. The molar extinction coefficient was determined (epsilon = 0.56), and static and dynamic light scattering measurements enabled the average molecular weight (M(w) = 13919), the second virial coefficient (A(2) = 23.95 x 10(-)(5) mol cm(3) g(-)(2)), and the hydrodynamic radius (R(H) = 1.98 nm) to be determined. No conformational changes were observed by fluorescence and circular dichroism measurements in different buffers at pH 3, 4.6, 7, and 12, confirming the high pH stability of this protein. From MALDI-TOF analysis and after enzymatic digestion, it was found that this purified sample, extracted from the rapeseed variety Express, contained mainly isoform 2SS3_BRANA.
authors.affiliation: Unité de Physico-Chimie des Macromolécules and Unité de Recherches sur les Protéines Végétales et leurs Interactions, INRA, Rue de la Géraudière, 44316 Nantes Cedex 03, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schmidt
authors.forename: Denis
authors.initials: D
authors.lastname: Renard
authors.forename: David
authors.initials: D
authors.lastname: Rondeau
authors.forename: Pascal
authors.initials: P
authors.lastname: Richomme
authors.forename: Yves
authors.initials: Y
authors.lastname: Popineau
authors.forename: Monique Augustin-Viguier
authors.initials: MA
authors.lastname: Axelos
mesh_terms: D055516:2S Albumins, Plant; D029688:Brassica napus / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D013058:Mass Spectrometry; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry*; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry; D012542:Scattering, Radiation; D013057:Spectrum Analysis
chemical_list: D055516:2S Albumins, Plant; D010940:Plant Proteins; D020033:Protein Isoforms; C050363:napin protein, Brassica napus