title: Yeast orthologues associated with glycerol transport and metabolism.
abstract: Glycerol is a key compound in the regulation of several metabolic pathways in Saccharomyces cerevisiae. From this yeast most of the genes involved in glycerol consumption, production and transport are now available. Some of the mechanisms involving glycerol metabolism and transport are common to other yeasts. This work presents a search for GPD1/2, GUT1, GUP1/2 and FPS1 orthologues in a series of hemiascomycetous yeasts. All the genes cloned were able to complement S. cerevisiae mutant phenotypes and presented a high degree of similarity to the corresponding genes in this yeast. A phylogenetic analysis is presented. The allocation of GUP genes in the membrane bound O-acyl transferases (MBOAT) family is suggested as more consistent than their inclusion in the TC-DB/glycerol uptake family.
authors.affiliation: Centro de Biologia da Universidade do Minho (CB-UM)/Departamento de Biologia, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Luisa
authors.initials: L
authors.lastname: Neves
authors.forename: Rui
authors.initials: R
authors.lastname: Oliveira
authors.forename: Cândida
authors.initials: C
authors.lastname: Lucas
mesh_terms: D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D002175:Candida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005816:Genetic Complementation Test; D005990:Glycerol / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010843:Pichia / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D020068:Zygosaccharomyces / Q000201:enzymology / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004271:DNA, Fungal; C093263:FPS1 protein, S cerevisiae; C499609:GUP2 protein, S cerevisiae; C414103:Gup1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol