Elucidation of two O-chain structures from the lipopolysaccharide fraction of Agrobacterium tumefaciens F/1.
Agrobacterium tumefaciens F/1 produces two different O-chains, both are constituted of rhamnose and glucosamine: the less abundant has a linear disaccharidic repeating unit 3)-alpha-L-Rhap-(1-->3)-beta-D-GlcpNAc-(1--> and the second one 4)-alpha-L-Rhap-(1-->3)-beta-D-GlcpNAc-(1-->. The two intact antigenic moieties were studied in mixture by 2D NMR. Additional supporting data were obtained by periodate degradation, the major component was cleaved selectively, leading to a glucosamine glycoside, whereas the minor one was recovered unaffected.
De Castro
Cristina
C
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cinthia 4, 80126 Napoli, Italy. decastro@unina.it
Carannante
Anna
A
Lanzetta
Rosa
R
Nunziata
Rosa
R
Piscopo
Vincenzo
V
Parrilli
Michelangelo
M
D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000096:biosynthesis / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry
D019081:O Antigens; D010504:Periodic Acid; C009288:metaperiodate