title: Antigenic properties of phage displayed peptides comprising disulfide-bonded loop of the immunodominant region of HIV-1 gp41.
abstract: The HIV-1 envelope glycoprotein gp41 contains Cys(X)5Cys motif, which has been shown to elicit a strong antibody response in almost all HIV-1 infected individuals. This disulfide-bonded loop region is conserved in most retroviruses suggesting the existence of an essential function in virus life cycle. In this study, we displayed the peptides comprising 12 amino acids of the immunodominant loop of gp41 on the surface of M13 phage as N-terminal fusions to the minor coat protein pIII and major coat protein pVIII of the phage and demonstrated that cysteine loop containing peptide expressed on phage recognized 62 out of 63 (98.4%) HIV-1 positive samples but not control negative sera while phage bearing linear peptides detected 4-30% of HIV-1-positive sera. The main advantage of phage-based ELISA or other antibody detection-based diagnostic tests of HIV-infection to be used for massive screening in developing countries is the reproducible, simple, rapid and low-cost production of recombinant antigens.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, México, DF CP04510, México.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cano
authors.forename: Monica
authors.initials: M
authors.lastname: Viveros
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Acero
authors.forename: Tzipe
authors.initials: T
authors.lastname: Govezensky
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Munguia
authors.forename: Eric
authors.initials: E
authors.lastname: Gonzalez
authors.forename: Luis
authors.initials: L
authors.lastname: Soto
authors.forename: Goar
authors.initials: G
authors.lastname: Gevorkian
authors.forename: Karen
authors.initials: K
authors.lastname: Manoutcharian
mesh_terms: D001667:Binding, Competitive; D004220:Disulfides / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000737:chemistry / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000097:blood / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D008623:Mercaptoethanol / Q000494:pharmacology; D019151:Peptide Library; D019163:Reducing Agents / Q000494:pharmacology; D012680:Sensitivity and Specificity
chemical_list: D004220:Disulfides; D015483:HIV Antibodies; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D016056:Immunodominant Epitopes; D019151:Peptide Library; D019163:Reducing Agents; D008623:Mercaptoethanol