Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish.
Histone deacetylases interact with nucleosomes to facilitate the formation of transcriptionally repressed chromatin. In the present study, we show that histone deacetylase 1 (hdac-1) is expressed throughout embryonic development of the zebrafish. The expression of hdac-1 is ubiquitous in early embryos (2-16 hr postfertilization), but at later stages (36 and 48 hr postfertilization), it is primarily restricted to the branchial arches, fin bud mesenchyme, and hindbrain. We report the phenotypes of hdac-1 homozygous mutant embryos and embryos injected with an hdac-1 antisense morpholino. These embryos possess a complex phenotype affecting several embryonic structures. We observed developmental abnormalities in the heart and neural epithelial structures, including the retina and the loss of craniofacial cartilage and pectoral fins.
Pillai
Renjitha
R
Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5.
Coverdale
Louise E
LE
Dubey
Gaytri
G
Martin
C Cristofre
CC
D000423:Alcian Blue / Q000378:metabolism; D000818:Animals; D002356:Cartilage / Q000196:embryology*; D004622:Embryo, Mammalian / Q000187:drug effects; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism / Q000502:physiology*; D006720:Homozygote; D017403:In Situ Hybridization; D016254:Mutagenesis, Insertional; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012886:Skull / Q000196:embryology*; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism / Q000502:physiology*
D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins; C488363:HDAC1 protein, zebrafish; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D000423:Alcian Blue