Effect of magnetic bead agglomeration on Cytomagnetometric measurements.
Magnetic twisting cytometry (MTC) is a novel tool to measure cytoskeleton-associated cell functions by the use of ferromagnetic microbeads. Magnetic beads are either incorporated by living cells by phagocytic processes or attached to integrin receptors to the cell membrane. The magnetic beads are magnetized and aligned in a strong magnetic field pulse. The application of twisting forces allows to investigate mechanical properties (stiffness, viscoelasticity) of the cytoskeleton of living cells by analyzing the magnetic cell field. Incorporated magnetic beads undergo intracellular transport processes, which result in a loss of particle alignment and in a decay of the remanent magnetic cell field. This process, called relaxation, depends on the mechanical cytoskeletal properties and can directly visualize the intracellular energy of cellular transport processes. The preparation of spherical monodisperse ferromagnetic beads made it possible to understand the above-described processes using mathematical models. Experimental conditions with many magnetic particles per cell enhances the formation of aggregates because of the attractive forces between magnetic spheres, resulting in a change of magnetic properties and of hydrodynamic behavior. Due to mutual magnetization, the remanent magnetic moment of an aggregate is stronger compared to the same number of single particles. This implies a higher cell field. Additionally the relaxation is retarded because of the change in shape factor and in volume, which also implies a faulty estimation of intracellular transport energy. Magnetic particle twisting is less influenced. In summary, valuable cytomagnetometric measurements have to be done with less than one particle per macrophage to ensure low probability of multiple particles per cell.
Möller
Winfried
W
GSF National Research Center for Environment and Health, Institute for Inhalation Biology, D-82131 Munich, Germany. moeller@gsf.de
Nemoto
Iku
I
Heyder
Joachim
J
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure*; D004058:Diffusion; D005290:Ferric Compounds; D008264:Macrophages / Q000166:cytology* / Q000502:physiology*; D008280:Magnetics / Q000295:instrumentation; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D010316:Particle Size
D005290:Ferric Compounds