Effects of amide-protected and lipid-encapsulated conjugated linoleic acid supplements on milk fat synthesis.
The trans-10, cis-12 isomer of conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis; its ability to reduce milk fat output in a controlled manner as a feed supplement, has potential management applications in the dairy industry. The effectiveness of dietary supplements of trans-10, cis-12 CLA is related to the extent to which their metabolism by rumen bacteria is minimized. A number of processes have been used to manufacture "rumen-protected" feed supplements, and their efficacy can be described by the extent of protection from rumen bacteria as well as postruminal bioavailability. The objective of this study was to investigate the effects of 2 rumen-protected CLA supplements on milk fat synthesis. Using the same initial batch of CLA, supplements were manufactured by the formation of fatty acyl amide bonds or by lipid encapsulation. Three rumen fistulated Holstein cows were randomly assigned in a 3 x 3 Latin square experiment. Treatments were 1) no supplement (control), 2) amide-protected CLA supplement, and 3) lipid-encapsulated CLA supplement. Supplements were fed to provide 10 g/d of the trans-10, cis-12 CLA isomer. Over the 7-d treatment period, 21 and 22% reductions in milk fat yield were observed for the amide-protected and lipid-encapsulated supplements, respectively. Transfer of trans-10, cis-12 CLA into milk fat was also similar for the amide-protected (7.1%) and lipid-encapsulated (7.9%) supplements. Overall, the amide-protected and lipid-encapsulated CLA supplements were equally effective at reducing milk fat synthesis and had no effect on milk yield or dry matter intake.
Perfield
J W
JW
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
Lock
A L
AL
Pfeiffer
A M
AM
Bauman
D E
DE
D000577:Amides / Q000737:chemistry*; D000818:Animals; D002214:Capsules; D002417:Cattle / Q000378:metabolism*; D019587:Dietary Supplements; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006865:Hydrogenation; D007774:Lactation; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D008055:Lipids / Q000096:biosynthesis*; D008892:Milk / Q000737:chemistry*; D012417:Rumen / Q000378:metabolism; D013024:Soybean Oil / Q000737:chemistry; D013229:Stearic Acids / Q000032:analysis
D000577:Amides; D002214:Capsules; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D013229:Stearic Acids; C031183:stearic acid; D013024:Soybean Oil