[Combining of TNP-470 and 5-Fu in inhibition of adenoid cystic carcinoma in nude mice model].
OBJECTIVE
To study the effect of angiogenesis inhibitor and its combine with chemical drug in suppressing the growth of adenoid cystic carcinoma (ACC).


METHODS
Acc-M cells were inoculated subcutaneous into BABL/C nu/nu mice. The mice were divided into control, different dose of TNP-470 treatment groups, 5-Fu treatment group and TNP-470 plus 5-Fu treatment group. Treatments were given 48 hours after inoculation. The mice were sacrificed on the 22nd day and excised tumors were weighted. Tumors were also investigated by immunohistochemistry and ultrastructural observations.


RESULTS
TNP-470 100 mg/kg/qod efficiently inhibited the growth of Acc-M tumors. TNP-470 30 mg/kg/qod combined with 50 mg/kg/week 5-Fu also resulted in significant growth inhibit of the tumors. TNP-470 suppressed tumor growth by inhibiting neovascularization, therefore inducing apoptosis of Acc-M cells. All experimental groups had different degrees of VEGF and bFGF express.


CONCLUSIONS
Since ACC is a slow developing tumor, blood supply is not so sufficient as sarcomas. Angiogensis inhibitor may inhibit its growth in high dosage. Combining medium dosage of angiogensis inhibitor with chemical drug may have synergistic result in inhibiting ACC growth.
Nong
Xiao-lin
XL
Dept. of Oral and Maxillofacial, Stomatology College, Guangxi Medical University, Nanning 530021, China.
Wang
Da-zhang
DZ
Meng
Min
M
Zhou
Nuo
N
Meng
Ning
N
Li
Jia-quan
JQ
Zhang
Hong
H
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy*; D045744:Cell Line, Tumor; D003510:Cyclohexanes / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000077609:O-(Chloroacetylcarbamoyl)fumagillol; D012717:Sesquiterpenes / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D003510:Cyclohexanes; D012717:Sesquiterpenes; D005472:Fluorouracil; D000077609:O-(Chloroacetylcarbamoyl)fumagillol