title: Identification of a novel Xenopus laevis poly (A) binding protein.
abstract: Poly (A) binding proteins are intimately implicated in controlling a number of events in mRNA metabolism from nuclear polyadenylation to cytoplasmic translation and stability. The known poly(A) binding proteins can be divided into three distinct structural groups (prototypes PABP1, PABPN1/PABP2 and Nab2p) and two functional families, showing that similar functions can be accomplished by differing structural units. This has prompted us to perform a screen for novel poly(A) binding proteins using Xenopus laevis. A novel poly(A) binding protein of 32 kDa (p32) was identified. Sequence analysis showed that p32 has about 50% identity to the known nuclear poly(A) binding proteins (PABPN1) but is more closely related to a group of mammalian proteins of unknown function. The expression of Xenopus laevis ePABP2 is restricted to early embryos. Accordingly, we propose that p32 is the founder member of a novel class of poly(A) binding proteins named ePABP2.
authors.affiliation: UMR6061 CNRS--Génétique et Développement, Université de Rennes 1, Faculté de Médecine, 2 avenue Professeur Leon Bernard, CS 34317, 35043 Rennes cedex, France. bertrand.cosson@univ-rennes1.fr
authors.forename: Bertrand
authors.initials: B
authors.lastname: Cosson
authors.forename: Frederique
authors.initials: F
authors.lastname: Braun
authors.forename: Luc
authors.initials: L
authors.lastname: Paillard
authors.forename: Perry
authors.initials: P
authors.lastname: Blackshear
authors.forename: H
authors.initials: H
authors.lastname: Beverley Osborne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D039102:Poly(A)-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: C496137:PABPN1L protein, Xenopus; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins