title: Multiparameter analysis for discreet differential diagnosis of mucosa-associated lymphoid tissue lymphoma in the intestine.
abstract: BACKGROUND
  The diagnosis of mucosa-associated lymphoid tissue (MALT) lymphoma in the intestine is occasionally difficult from histological examination on small biopsy specimens obtained by endoscopy. This study focused on unusual cases of reactive lymphoproliferative disorders in the intestine in order to make a differential diagnosis of MALT lymphoma.
  
  
  METHODS
  Five patients were examined with regards to clinical symptoms, endoscopic findings and multiparameter analysis (the morphological examination using routine hematoxylin and eosin staining by light microscopy, immunophenotyping by flow cytometry (FCM), immunohistochemistry and genotyping of extracted DNA).
  
  
  RESULTS
  All cases showed an aggregation of lymphocytes and one case showed similar features to lymphoepithelial lesions. Analyses of FCM and genetic rearrangements denied the monoclonality in all cases. Consequently, we considered that all cases should be diagnosed as reactive lymphoid hyperplasia and inflammatory change.
  
  
  CONCLUSIONS
  Multiparameter analysis is useful in making an exact diagnosis of MALT lymphoma and therefore contributes to prevent unnecessary overtreatment.
authors.affiliation: Tohoku University, Graduate School of Medicine, Sendai, Japan. enomura@int3.med.tohoku.ac.jp
authors.forename: Eiki
authors.initials: E
authors.lastname: Nomura
authors.forename: Sho
authors.initials: S
authors.lastname: Takagi
authors.forename: Ryo
authors.initials: R
authors.lastname: Ichinohasama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Manabu
authors.initials: M
authors.lastname: Shiraki
authors.forename: Shinya
authors.initials: S
authors.lastname: Oomori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Utsunomiya
authors.forename: Kenichi
authors.initials: K
authors.lastname: Negoro
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aihara
authors.forename: Seiichi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kinouchi
authors.forename: Tooru
authors.initials: T
authors.lastname: Shimosegawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004273:DNA, Neoplasm / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D007414:Intestinal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis
chemical_list: D004273:DNA, Neoplasm