Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities.
Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract-malt (YM) broth. Autolysis of the HA-2 mutant at 60 degrees C and pH 7.0 for 6 h was the best condition to obtain maximum yields of 5'-ribonucleotides, inosinic monophosphate (IMP) (6.2 mg/g biomass) and guanylic monophosphate (GMP) (35.5 mg/g biomass). The yield of adenylic monophosphate (AMP) (7.8 mg/g biomass) was optimal at 60 degrees C at pH 6.5 for 6 h. The inhibitory activity of the angiotensin-converting enzyme and the nitrite-scavenging activity for autolysates of the HA-2 mutant were about 13.0% and 47.0% higher than those of native strain, respectively.
Lee
Jong-Soo
JS
Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Daejon, Korea.
Hyun
Kwang-Wook
KW
Jeong
Seung-Chan
SC
Kim
Jae-Ho
JH
Choi
Young J
YJ
Miguez
Carlos B
CB
D000249:Adenosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis; D000935:Antifungal Agents / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis; D006157:Guanosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007291:Inosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D011189:Potassium Chloride / Q000494:pharmacology; D012265:Ribonucleotides / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D000806:Angiotensin-Converting Enzyme Inhibitors; D000935:Antifungal Agents; D016166:Free Radical Scavengers; D009153:Mutagens; D012265:Ribonucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D011189:Potassium Chloride; D006157:Guanosine Monophosphate; D003513:Cycloheximide; D005020:Ethyl Methanesulfonate