The potential value of (Myo)fibroblastic stromal reaction in the diagnosis of prostatic adenocarcinoma.
BACKGROUND
Reactive stromal changes that occur in different human cancers probably are involved in local tumor spreading. The aim of this study was to analyze changes of the stroma in prostatic carcinoma and to establish their possible diagnostic significance.


METHODS
For the definition of reactive stroma in prostate cancer Mallory trichrome method and immunohistochemical analysis of vimentin, alpha-smooth muscle actin, and desmin were performed.


RESULTS
Compared with adjacent peritumoral tissue and stroma in benign prostatic hyperplasia, reactive stroma in prostate cancer showed increased vimentin expression, and decreased desmin expression (P < 0.05). Mallory method also showed more pronounced desmoplastic reaction in stroma of prostatic adenocarcinoma (P < 0.05).


CONCLUSIONS
Our study indicates that a reactive stroma, which occurs in prostatic carcinoma, could be clearly shown by Mallory method and confirmed by immunohistochemistry. We suggest that Mallory trichrome method may be used as a helpful additional diagnostic tool in the diagnosis of suspicious cases of prostatic adenocarcinoma.
Tomas
Davor
D
Department of Pathology, Sestre milosrdnice University Hospital, Ljudevit Jurak University, Zagreb, Croatia.
Kruslin
Bozo
B
D000199:Actins / Q000032:analysis; D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D000368:Aged; D001391:Azo Compounds; D004396:Coloring Agents; D003893:Desmin / Q000032:analysis; D003937:Diagnosis, Differential; D004801:Eosine Yellowish-(YS); D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008739:Methyl Green; D008875:Middle Aged; D009130:Muscle, Smooth / Q000737:chemistry / Q000473:pathology; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D017154:Stromal Cells / Q000737:chemistry* / Q000473:pathology*; D014746:Vimentin / Q000032:analysis
D000199:Actins; D001391:Azo Compounds; D004396:Coloring Agents; D003893:Desmin; D014746:Vimentin; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)