Detection of kaposi sarcoma-associated herpesvirus DNA in saliva and buffy-coat samples from children with sickle cell disease in Uganda.
Among 233 children, Kaposi sarcoma-associated herpesvirus (KSHV) DNA was detected in 43% of children seropositive for both K8.1 and orf73, in 29% of children seropositive for K8.1 only, in 14% of children seropositive for orf73 only, and in 7% of children seronegative for both K8.1 and orf73; among 228 mothers, KSHV DNA was detected in 27%, 25%, 4%, and 1%, respectively. KSHV DNA was detected more frequently and at higher levels in saliva than in buffy-coat samples and in children than in mothers. In both children and mothers, detection in saliva was associated with detection in peripheral blood. Detection was associated with K8.1 seropositivity, younger age, and high household density, indicating the importance of in-household person-to-person transmission, likely via saliva.
Mbulaiteye
Sam M
SM
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Rockville, Maryland, USA. mbulaits@mail.nih.gov.
Pfeiffer
Ruth M
RM
Engels
Eric A
EA
Marshall
Vickie
V
Bakaki
Paul M
PM
Owor
Anchilla M
AM
Ndugwa
Christopher M
CM
Katongole-Mbidde
Edward
E
Goedert
James J
JJ
Biggar
Robert J
RJ
Whitby
Denise
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D000755:Anemia, Sickle Cell / Q000097:blood / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D018562:Disease Transmission, Infectious; D005260:Female; D006566:Herpesviridae Infections / Q000097:blood / Q000635:transmission* / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000821:virology*; D014454:Uganda
D000914:Antibodies, Viral; D004279:DNA, Viral