title: In vivo function of a gammaherpesvirus virion glycoprotein: influence on B-cell infection and mononucleosis.
abstract: The human gammaherpesviruses Epstein-Barr virus and Kaposi Sarcoma-associated herpesvirus both contain a glycoprotein (gp350/220 and K8.1, respectively) that mediates binding to target cells and has been studied in great detail in vitro. However, there is no direct information on the role that these glycoproteins play in pathogenesis in vivo. Infection of mice by murid herpesvirus 4 strain 68 (MHV-68) is an established animal model for gammaherpesvirus pathogenesis and expresses an analogous glycoprotein, gp150. To elucidate the in vivo function of gp150, a recombinant MHV-68 deficient in gp150 production was generated (vgp150Delta). The productive viral replication in vitro and in vivo was largely unaffected by mutation of gp150, aside from a partial defect in the release of extracellular virus. Likewise, B-cell latency was established. However, the transient mononucleosis and spike in latently infected cells associated with the spread of MHV-68 to the spleen was significantly reduced in vgp150Delta-infected mice. A soluble, recombinant gp150 was found to bind specifically to B cells but not to epithelial cells in culture. In addition, gp150-deficient MHV-68 derived from mouse lungs bound less well to spleen cells than wild-type virus. Thus, gp150 is highly similar in function in vitro to the Epstein-Barr virus gp350/220. These results suggest a role for these analogous proteins in mononucleosis and have implications for their use as vaccine antigens.
authors.affiliation: Centre for Comparative Infectious Diseases, Department of Medical Microbiology, University of Liverpool, Duncan Building, Daulby St., Liverpool L69 3GA, United Kingdom. j.p.stewart@liv.ac.uk
authors.forename: James P
authors.initials: JP
authors.lastname: Stewart
authors.forename: Ondine J
authors.initials: OJ
authors.lastname: Silvia
authors.forename: Isobel M D
authors.initials: IM
authors.lastname: Atkin
authors.forename: David J
authors.initials: DJ
authors.lastname: Hughes
authors.forename: Bahram
authors.initials: B
authors.lastname: Ebrahimi
authors.forename: Heiko
authors.initials: H
authors.lastname: Adler
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D004195:Disease Models, Animal; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D006566:Herpesviridae Infections / Q000821:virology*; D007244:Infectious Mononucleosis / Q000821:virology*; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018144:Rhadinovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013154:Spleen / Q000821:virology; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins