title: Caveolin-stabilized membrane domains as multifunctional transport and sorting devices in endocytic membrane traffic.
abstract: Endocytosis comprises several routes of internalization. An outstanding question is whether the caveolar and endosomal pathways intersect. Following transport of the caveolar protein Caveolin-1 and two cargo complexes, Simian Virus 40 and Cholera toxin, in live cells, we uncovered a Rab5-dependent pathway in which caveolar vesicles are targeted to early endosomes and form distinct and stable membrane domains. In endosomes, the low pH selectively allowed the toxin to diffuse out of the caveolar domains into the surrounding membrane, while the virus remained trapped. Thus, we conclude that, unlike cyclic assembly and disassembly of coat proteins in vesicular transport, oligomeric complexes of caveolin-1 confer permanent structural stability to caveolar vesicles that transiently interact with endosomes to form subdomains and release cargo selectively by compartment-specific cues.
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany. pelkmans@mpi-cbg.de
authors.forename: Lucas
authors.initials: L
authors.lastname: Pelkmans
authors.forename: Thomas
authors.initials: T
authors.lastname: Bürli
authors.forename: Marino
authors.initials: M
authors.lastname: Zerial
authors.forename: Ari
authors.initials: A
authors.lastname: Helenius
mesh_terms: D021941:Caveolae / Q000378:metabolism* / Q000648:ultrastructure; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D002451:Cell Compartmentation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002772:Cholera Toxin / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D021381:Protein Transport / Q000502:physiology; D013539:Simian virus 40 / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D002772:Cholera Toxin; D020696:rab5 GTP-Binding Proteins