title: Differentiation of Actinobacillus pleuropneumoniae by PCR-REA based on sequence variability of the apxIVA gene and by ribotyping.
abstract: During the period of 2001-2003, a total of 591 Actinobacillus pleuropneumoniae field isolates from the Czech Republic were serotyped with a high occurrence of cross-reactions. The cross-reactions were observed in 416 isolates. Most frequently, in 401 isolates (67.9%), cross-reactions with antisera specific for serotypes 9, 11, and/or 1 were observed. Two additional molecular methods, ribotyping and restriction analysis of PCR amplified apxIVA gene (PCR-REA), were therefore used for detailed characterisation of A. pleuropneumoniae. In this subsequent analysis, reference strains representing serotypes 1-12 and 25 field isolates showing the most frequent serotype cross-reactions were examined. PCR-REA enabled all reference strains to be distinguished except for the strains of serotypes 9 and 11. Ribotyping distinguished all reference strains except two pairs of serotypes: 3 versus 6, and 9 versus 11, respectively. Field isolates with serotype cross-reactivity 9, 11, and/or 1 could not be differentiated by either of these methods.
authors.affiliation: Veterinary Research Institute Brno, Hudcova 70, 621 32 Brno, Czech Republic. jaglic@vri.cz
authors.forename: Zoran
authors.initials: Z
authors.lastname: Jaglic
authors.forename: Petra
authors.initials: P
authors.lastname: Svastova
authors.forename: Ivan
authors.initials: I
authors.lastname: Rychlik
authors.forename: Katerina
authors.initials: K
authors.lastname: Nedbalcova
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kucerova
authors.forename: Ivo
authors.initials: I
authors.lastname: Pavlik
authors.forename: Milan
authors.initials: M
authors.lastname: Bartos
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003429:Cross Reactions; D018153:Czech Republic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015183:Restriction Mapping / Q000662:veterinary; D021521:Ribotyping / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D004269:DNA, Bacterial