title: Relationship between phenotypic and genotypic florfenicol resistance in Escherichia coli.
abstract: This study evaluated the relationship between florfenicol resistance and flo genotypes in 1,987 Escherichia coli isolates from cattle. The flo gene was detected in 164 isolates, all of which expressed resistance to florfenicol at MICs of >/=256 microg/ml. The florfenicol MICs for all isolates that lacked flo were </=16 microg/ml.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 300A VSB, 1971 Commonwealth Ave., St. Paul, MN 55108, USA. singe024@umn.edu
authors.forename: Randall S
authors.initials: RS
authors.lastname: Singer
authors.forename: Sheila K
authors.initials: SK
authors.lastname: Patterson
authors.forename: Anne E
authors.initials: AE
authors.lastname: Meier
authors.forename: Jessica K
authors.initials: JK
authors.lastname: Gibson
authors.forename: Hannah L
authors.initials: HL
authors.lastname: Lee
authors.forename: Carol W
authors.initials: CW
authors.lastname: Maddox
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002702:Chloramphenicol Resistance / Q000235:genetics; D003227:Conjugation, Genetic; D017931:DNA Primers; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005838:Genotype; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; C035534:florfenicol; D013839:Thiamphenicol