Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice.
Wild-type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle-T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle-T-transformed mouse bEND cells prepared from PECAM-1-/- mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM-1+/+ bEND cells. PECAM-1-/- bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM-1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM-1+/+ bEND cells. Therefore, lack of PECAM-1 expression impacts polyoma middle-T-transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary-like structures in Matrigel.
Rothermel
Terri A
TA
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin 53792-4673, USA.
Engelhardt
Britta
B
Sheibani
Nader
N
D000818:Animals; D015703:Antigens, CD; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002472:Cell Transformation, Viral / Q000235:genetics*; D003094:Collagen; D004338:Drug Combinations; D042783:Endothelial Cells / Q000378:metabolism* / Q000821:virology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006391:Hemangioma / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D007797:Laminin; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D018995:Myosin Heavy Chains; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000235:genetics; D024501:Nonmuscle Myosin Type IIB; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism; D011120:Polyomavirus / Q000378:metabolism*; D011509:Proteoglycans; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D000099152:Cadherin 5
D015703:Antigens, CD; D000957:Antigens, Viral, Tumor; D015415:Biomarkers; D015820:Cadherins; D003094:Collagen; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D007797:Laminin; D018995:Myosin Heavy Chains; D024501:Nonmuscle Myosin Type IIB; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D019010:Vascular Cell Adhesion Molecule-1; D040301:Vascular Endothelial Growth Factor Receptor-2; D000099152:Cadherin 5; C059485:matrigel; C103815:nonmuscle myosin type IIB heavy chain