Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris.
The galactose-binding lectin EW29 from the earthworm Lumbricus terrestris is composed of two homologous domains, both of which are members of the R-type lectin family. The truncated mutant rC-half comprising the C-terminal domain was crystallized by the hanging-drop vapour-diffusion method. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 61.2, c = 175.6 A, and diffracted to beyond 1.9 A resolution. Matthews coefficient calculations suggested that this crystal contained two molecules per asymmetric unit.
Suzuki
Ryuichiro
R
Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.
Fujimoto
Zui
Z
Kuno
Atsushi
A
Hirabayashi
Jun
J
Kasai
Ken-ichi
K
Hasegawa
Tsunemi
T
D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods; D005690:Galactose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D009835:Oligochaeta / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012984:Software; D014961:X-Ray Diffraction
D037102:Lectins; D005690:Galactose