Dietary daidzein influences laying performance of ducks (Anas platyrhynchos) and early post-hatch growth of their hatchlings by modulating gene expression.
Our previous studies demonstrated that dietary supplementation of daidzein improves egg production in duck breeders during late periods of the laying cycle. The present study was aimed to clarify whether the growth of ducklings hatched from eggs laid by daidzein-treated hens would be affected, and to elucidate the mechanisms underlying potential trans-generational effects, by determining changes of hormone levels and mRNA expression of relevant genes. Daidzein was added to the basal diet of 415-day-old duck breeders at the level of 5 mg/kg. During 9 weeks of daidzein treatment, laying rate increased by 7.70%, average egg mass tended to increase, whereas yolk/albumen ratio decreased significantly. These changes were accompanied by significantly elevated plasma T4 and E2 levels, enhanced gonadotropin releasing hormone (GnRH) mRNA, but diminished estrogen receptor (ER)-beta mRNA expression in hypothalamus of daidzein-treated hens. Ducklings hatched from daidzein-treated eggs were significantly smaller at hatching, but they caught up with their control counterparts by 4 weeks of age. Serum levels of T4, pituitary GH, hepatic GH receptor (GHR) and type-1 IGF receptor (IGF-1R) mRNA expression were all suppressed markedly in the daidzein-treated group at hatching, but this suppression proved to be temporary, as at 4 weeks of age, expression levels of all investigated genes were restored. However, it is noteworthy that at 4 weeks of age an obvious down-regulation of hypothalamic GnRH mRNA expression was detected in ducklings maternally exposed to daidzein. Our results provide evidence that maternal exposure to daidzein affects post-hatch growth in the duck with accompanying changes in the secretion of metabolic hormones and expression of growth-related genes. Although the negative effect of maternal daidzein on embryonic growth could be eliminated 4 weeks after hatching, the long-term effect of maternal daidzein on reproductive function is not to be ignored and awaits further investigation.
Zhao
Ruqian
R
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China. yzwj@public1.ptt.js.cn
Wang
Yaju
Y
Zhou
Yuchuan
Y
Ni
Yingdong
Y
Lu
Lizhi
L
Grossmann
Roland
R
Chen
Jie
J
D000375:Aging / Q000235:genetics / Q000502:physiology*; D000821:Animal Feed; D000818:Animals; D004032:Diet; D004372:Ducks / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D004531:Eggs; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007529:Isoflavones / Q000008:administration & dosage* / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018811:Maternal Exposure; D010058:Oviposition / Q000187:drug effects*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D011986:Receptors, Somatotropin / Q000235:genetics; D013997:Time Factors
D007529:Isoflavones; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011986:Receptors, Somatotropin; D007987:Gonadotropin-Releasing Hormone; C004742:daidzein; D013006:Growth Hormone; D017526:Receptor, IGF Type 1