Genetic organisation of the capsule transport gene region from Haemophilus paragallinarum.
The region involved in export of the capsule polysaccharides to the cell surface of Haemophilus paragallinarum was cloned and the genetic organisation determined. Degenerate primers designed from sequence alignment of the capsule transport genes of Haemophilus influenzae, Pasteurella multocida and Actinobacillus pleuropneumoniae were used to amplify a 2.6 kb fragment containing a segment of the H. paragallinarum capsule transport gene locus. This fragment was used as a digoxigenin labelled probe to isolate the complete H. paragallinarum capsule transport gene locus from genomic DNA. The sequence of the cloned DNA was determined and analysis revealed the presence of four genes, each showing high homology with known capsule transport genes. The four genes were designated hctA, B, C and D (for H. paragallinarum capsule transport genes) and the predicted products of these genes likely encode an ATP-dependent export system responsible for transport of the capsule polysaccharides to the cell surface, possibly a member of a super family designated ABC (ATP-binding cassette) transporters.
De Smidt
O
O
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State PO. Box 339, Bloemfontein, 9300 South Africa.
Albertyn
J
J
Bragg
R R
RR
Van Heerden
E
E
D000595:Amino Acid Sequence; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary; D044134:Haemophilus paragallinarum / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016415:Sequence Alignment
D004269:DNA, Bacterial