title: [DNA content and survival in subserous gallbladder carcinoma].
abstract: BACKGROUND
  The clinical and morphological characterization of the subserous gallbladder carcinomas is controversial.
  
  
  OBJECTIVE
  To study the prognostic importance of DNA content of subserous gallbladder carcinoma.
  
  
  METHODS
  We studied 104 females aged 60+/-12 years old and 16 men aged 70+/-13 years old. In all of them diagnosis was established after mapping of cholecystectomy sample and had a complete clinical follow up. DNA content was measured by flow cytometry.
  
  
  RESULTS
  All tumors were adenocarcinoma, and only 16% were well differentiated. Aneuploidy was observed in 29 cases (26%) with DNA index fluctuating between 1.1 and 1.8. Lymphatic vessel tumor involvement was present in 16 of 22 cases with aneuploidy and in 22 of 46 diploid tumors (p=0.05). Eighty nine percent of aneuploid tumors were detected macroscopically and 11% were unapparent. Five years survival was non significantly better among patients with diploid tumors than in patients with aneuploid tumors (45 and 28%, respectively, p=0.2). The histological differentiation was the only variable significantly associated with survival.
  
  
  CONCLUSIONS
  Aneuploidy is present in 26% of subserous gallbladder carcinoma. It is not related with any of the morphological or clinical variables studied in this series of patients.
authors.affiliation: Departamento de Anatomía Patológica, Facultad de Medicina, Universidad de la Frontera. Iroa@ufro.cl
authors.forename: Iván
authors.initials: I
authors.lastname: Roa
authors.forename: Xabier
authors.initials: X
authors.lastname: de Aretxabala
authors.forename: Patricia
authors.initials: P
authors.lastname: Fuentealba
authors.forename: María Elena
authors.initials: ME
authors.lastname: Cabrera
authors.forename: Juan C
authors.initials: JC
authors.lastname: Araya
authors.forename: Juan
authors.initials: J
authors.lastname: Roa
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000782:Aneuploidy; D002677:Chile / Q000453:epidemiology; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D004273:DNA, Neoplasm
vernacular_title: Contenido de ADN y sobrevida en el carcinoma subseroso de la vesícula biliar.