Visceral schistosomiasis of domestic animals in India: humoral immune status of infected cattle, sheep and goats against major polypeptide antigens of Schistosoma indicum and S. spindale.
Polypeptide profiles of Schistosoma indicum and S. spindale adult worm homogenates were obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Humoral immune status of infected cattle, sheep and goats against Schistosoma indicum and S. spindale Ags was determined by immunoblot analysis and by indirect ELISA using four major polypeptides of approximate molecular masses 45 kDa, 40 kDa, 28 kDa and 15 kDa electro-eluted from the gel slices. Cattle sera samples had higher levels of antibodies against Si/s40 and Si/s28 than against Si/s45 antigen. Reasons have been discussed for the absence of detectable levels of anti-Si/s28, -Si/s45 and -Si/s40 antibodies in a significant number of sera samples from S. indicum egg-positive sheep.
Singh
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Immunology Section, Department of Veterinary Microbiology, Ch. Charan Singh Haryana Agricultural University, Hisar (Haryana), India.
Singh
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Chaudhri
S S
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D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D015511:Goat Diseases / Q000276:immunology / Q000469:parasitology; D006041:Goats; D015151:Immunoblotting; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D012547:Schistosoma / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D010455:Peptides