title: Expression patterns of beta1-related alpha integrin subunits in murine lens during embryonic development and wound healing.
abstract: OBJECTIVE
  To study the expression patterns of b1-related alpha integrin subunits in murine lens epithelial cells, comparing embryonic fiber differentiation with injury-induced epithelial mesenchymal transition (EMT).
  
  
  METHODS
  Adult mice type C57BL/6, pregnant as well as with an eye injured, were sacrificed at different time-course intervals. The embryonic and the injured eyes were obtained and deparaffinized sections of these eyes were processed for immunohistochemistry staining for detection of integrin a subunits.
  
  
  RESULTS
  Embryonic lens epithelial cells expressed primarily a3 and a5 subunits, whereas embryonic fiber cells expressed a2, a5, and a6 subunits. Adult lens epithelial cells expressed a3, and a6 subunits,whereas injured lens cells expressed a2, a3, and a6 integrin subunits.
  
  
  CONCLUSIONS
  The phenotypic changes of lens epithelial cells during embryonic fiber differentiation and EMT are characterized by different expression of integrin subunits as a result both of the altered extracellular matrix conditions and of the altered cell signaling pathways recruited in each process.
authors.affiliation: Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, Japan. w.barbour@mailcity.com
authors.forename: Walid
authors.initials: W
authors.lastname: Barbour
authors.forename: Shizuya
authors.initials: S
authors.lastname: Saika
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Ohkawa
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000378:metabolism; D005131:Eye Injuries / Q000378:metabolism*; D005260:Female; D007124:Immunoenzyme Techniques; D039001:Integrin alpha Chains / Q000378:metabolism*; D007908:Lens, Crystalline / Q000196:embryology* / Q000293:injuries* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D014945:Wound Healing
chemical_list: D039001:Integrin alpha Chains