title: Role of follicular dendritic cells in the early HIV-1 infection: in vitro model without specific antibody.
abstract: About 90% of HIV-1 RNA in the lymph nodes is reported to localize in follicular dendritic cellsnetwork (FDC-NW) as early as several days after infection and as much as that in the late stage. But the mechanism remains to be fully understood. To elucidate the role of follicular dendritic cells (FDC) in the early stage of HIV-1 infection, FDC-like cell strains (FDCLC) were established and they were characterized in the co-culture system with T cells for their effect on HIV-1 trapping and replication in p24 immunoassay, immunohistochemistry as well as confocal and electronmicroscopy. Established FDCLC were positive for CNA-42, S-100alpha and intercellular desmosome-like junctions. L-SIGN and DC-SIGN were also detected in FDCLC. Alu-HIV-1 PCR analysis showed no HIV-1 integration in FDCLC. FDCLC trapped HIV-1 and transferred them to uninfected MOLT-4 T cells (MOLT-4) efficiently in the absence of specific antibody. FDCLC also accelerated HIV-1 replication in HIV-1-pre-exposed MOLT-4. These unique FDCLC effects were explained, at least partly, by the fact that FDCLC up-regulated CD4 expression in MOLT-4 and helped T cells escape from apoptosis in the co-culture. These data suggest that FDC/FDCLC engage not only in trapping but also in active expansion of HIV-1 in the absence of specific antibody.
authors.affiliation: Department of Molecular Virology, Bio-Response, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Taruishi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Terashima
authors.forename: Zahidunnabi
authors.initials: Z
authors.lastname: Dewan
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Eishi
authors.forename: Momoko
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Tohru
authors.initials: T
authors.lastname: Furusaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Masanori
authors.initials: M
authors.lastname: Ishii
authors.forename: Ken
authors.initials: K
authors.lastname: Kitamura
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D020566:Dendritic Cells, Follicular / Q000502:physiology* / Q000821:virology*; D004197:Disease Reservoirs; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D013601:T-Lymphocytes / Q000821:virology*; D014779:Virus Replication