title: Effects of feeding and body weight loss on the 1H-NMR-based urine metabolic profiles of male Wistar Han rats: implications for biomarker discovery.
abstract: For almost two decades, 1H-NMR spectroscopy has been used as an 'open' system to study the temporal changes in the biochemical composition of biofluids, including urine, in response to adverse toxic events. Many of these in vivo studies have reported changes in individual metabolites and patterns of metabolites that correlated with toxicological changes. However, many of the proposed novel biomarkers are common to a number of different types of toxicity. These may therefore reflect non-specific effects of toxicity, such as weight loss, rather than a specific pathology. A study was carried out to investigate the non-specific effects on urinary metabolite profiles by administering four hepatotoxic compounds, as a single dose, to rats at two dose levels: hydrazine hydrate (0.06 or 0.08 g kg (1)), 1,2-dimethylhydrazine (0.1 or 0.3 g kg (-1)), alpha-napthylisothiocyanate (0.1 or 0.15 g kg(-1)) and carbon tetrachloride (1.58 or 3.16 g kg(-1)). The study included weight-matched control animals along with those that were dosed, which were then 'pair-fed' with the treated animals so they achieved a similar weight loss. The urinary metabolite profiles were investigated over time using 1H-NMR spectroscopy and compared with the pathology from the same animals. The temporal changes were analysed statistically using multivariate statistical data analysis including principal component analysis, partial least squares, parallel factor analysis and Fisher's criteria. A number of metabolites associated with energy metabolism or which are partially dietary in origin, such as creatine, creatinine, tricarboxylic acid (TCA) cycle intermediates, phenylacetylglycine, fumarate, glucose, taurine, fatty acids and N-methylnicotinamide, showed altered levels in the urine of treated and pair-fed animals. Many of these changes correlated well with weight loss. Interestingly, there was no increase in ketone bodies (acetate and beta-hydroxybutyrate), which might be expected if energy metabolism was switched from glycolysis to fatty acid beta-oxidation. In some instances, the metabolites that changed were considered to be non-specific markers of toxicity, but were also identified as markers of a specific type of toxicity. For example, taurine was raised significantly in carbon tetrachloride-treated animals but reduced in the pair-fed group. However, raised urinary bile acid levels were only seen after alpha-napthylisothiocyanate treatment. The methodology, statistical analysis used and the data generated will help improve the identification of specific markers or patterns of urinary markers of specific toxic effects.
authors.affiliation: Safety Assessment, GlaxoSmithKline Research and Development Ltd, Ware, UK.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Connor
authors.forename: Wen
authors.initials: W
authors.lastname: Wu
authors.forename: Brian C
authors.initials: BC
authors.lastname: Sweatman
authors.forename: Jodi
authors.initials: J
authors.lastname: Manini
authors.forename: John N
authors.initials: JN
authors.lastname: Haselden
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Crowther
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Waterfield
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000494:pharmacology; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis / Q000652:urine*; D004734:Energy Metabolism / Q000187:drug effects*; D006834:Hydrazines / Q000008:administration & dosage / Q000494:pharmacology; D017879:Isothiocyanates / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D051381:Rats; D017208:Rats, Wistar; D014556:Urine / Q000737:chemistry*; D015431:Weight Loss
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D006834:Hydrazines; D017879:Isothiocyanates; C029424:hydrazine; C037152:isothiocyanic acid; D002251:Carbon Tetrachloride; D019813:1,2-Dimethylhydrazine