title: Glycogenolysis during short-term fasting in malaria and healthy subjects--the potential regulatory role of glycogen content on glycogen breakdown: a hypothesis.
abstract: OBJECTIVE
  During short-term starvation (< 24 h), glucose production decreases 10-20% due to a decrease in glycogenolysis. In the fed state glycogen regulates its rate of breakdown, in order to limit glycogen accumulation. Whether in the fasted state a similar mechanism exists to preserve glycogen content is not known. In malaria, the rate of glycogen breakdown after an overnight fast is considerably lower than in healthy subjects. If glycogen content regulates its rate of breakdown during fasting, we postulate that the rate of glycogenolysis should decrease faster in patients with malaria than in healthy subjects.
  
  
  METHODS
  In six non-severe falciparum malaria patients and 6 healthy controls glucose production with [6,6-2H2]-glucose, and glycogenolysis was calculated after measuring gluconeogenesis with the 2H2O-method between 16 and 22 h of fasting.
  
  
  RESULTS
  Glucose production after 16 h of fasting was 15% higher in the malaria patients than in controls. Glycogenolysis in the malaria patients was 2.3 +/- 0.37 and 8.4 +/- 0.93 micromol/kg/min in the controls. The absolute decrease in glycogenolysis was slower in malaria patients than in controls (P = 0.001), whereas the relative decrease in glycogenolysis from baseline was not different.
  
  
  CONCLUSIONS
  During fasting the relative decrease in glycogenolysis is independent of the absolute rate of glycogenolysis. The regulation of glycogenolysis during fasting seems not preferentially dictated by glycogen content but, at least in subjects with a low (presumed) glycogen content, driven by the necessity to guarantee glucose output and maintain euglycemia.
authors.affiliation: Department of Endocrinology and Metabolism, Academic Medical Center, PO Box 22600, F5-170, Amsterdam 1100 DD, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Sprangers
authors.forename: H V
authors.initials: HV
authors.lastname: Thien
authors.forename: M T
authors.initials: MT
authors.lastname: Ackermans
authors.forename: E
authors.initials: E
authors.lastname: Endert
authors.forename: H P
authors.initials: HP
authors.lastname: Sauerwein
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D003903:Deuterium; D005215:Fasting / Q000378:metabolism*; D005260:Female; D005943:Gluconeogenesis; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D016778:Malaria, Falciparum / Q000378:metabolism*; D008297:Male; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D006003:Glycogen; D003903:Deuterium