ICAM-1 supports adhesion of human small-cell lung carcinoma to endothelial cells.
Adhesion of tumor cells to endothelium via cell-adhesion molecules constitutes a crucial step in metastasis, which is largely responsible for the poor prognosis of small-cell lung carcinoma (SCLC). Patients with SCLC were reported to have elevated levels of intercellular adhesion molecule-1 (ICAM-1). The present study therefore focusses on endothelial ICAM-1 in tumor-cell adhesion. We found that the adherence of SCLC cells (cell lines H24, H69, H82) to cultured vascular endothelium in stasis and flow depends on the expression of ICAM-1. After blocking endothelial ICAM-1 with monoclonal antibodies, adhesion was significantly reduced. These results pinpoint ICAM-1 for the first time as a molecule crucially involved in SCLC cell-endothelial adhesion.
Finzel
Angela H
AH
Anatomisches Institut der Universität München, München, Germany.
Reininger
Armin J
AJ
Bode
Patrick A
PA
Wurzinger
Laurenz J
LJ
D018288:Carcinoma, Small Cell / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology; D008175:Lung Neoplasms / Q000473:pathology*
D007375:Interleukin-1; D018799:Intercellular Adhesion Molecule-1