Fast separation and determination of phenolic compounds by capillary electrophoresis-diode array detection. Application to the characterisation of alperujo after ultrasound-assisted extraction.
A dynamic approach has been proposed for the ultrasound-assisted extraction of twenty phenolic compounds from alperujo, a semisolid waste from the olive oil industry, that is a representative example of samples with a complex matrix. Multivariate methodology was used to carry out a detailed optimisation study of both the separation-determination and extraction steps in terms of resolution-analysis time and extraction efficiency, respectively. Consequently, the proposed method was able to extract the target analytes in 13 min; then, after dilution and centrifugation, the extract was injected into the capillary electrophoresis-diode array detection system for individual separation determination in 11 min. No cleanup of the extract was required. This method is less time-consuming, more selective and provides a larger information level than the Folin-Ciocalteau spectrophotometric method. Alperujo was demonstrated to be a powerful source of phenolic compounds, particularly as compared with olive oil--8680 versus 50-1200 microg/g.
Priego-Capote
F
F
Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Ruiz-Jiménez
J
J
de Castro
M D Luque
MD
D019075:Electrophoresis, Capillary / Q000379:methods*; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results
D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils