Characterization of development-related genes for the cestode Bothriocephalus acheilognathi.
Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were upregulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and the three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.
Luo
H L
HL
State Key Laboratory of Freshwater Ecology and Biotechnology, Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei 430072 Wuhan, China.
Nie
P
P
Chang
M X
MX
Song
Y
Y
Yao
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002589:Cestoda / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development*; D002590:Cestode Infections / Q000469:parasitology / Q000662:veterinary; D003530:Cyprinidae / Q000469:parasitology*; D018076:DNA, Complementary; D005393:Fish Diseases / Q000469:parasitology; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D015801:Helminth Proteins