Induction of Mycobacterium avium proteins upon infection of human macrophages.
Induction of Mycobacterium avium proteins labelled with [35S]methionine and mRNAs upon infection of the human macrophage cell line THP-1 was investigated by two-dimensional gel electrophoresis-mass spectrometry and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. M. avium overexpressed proteins within the macrophages that are involved in fatty acids metabolism (FadE2, FixA), cell wall synthesis (KasA), and protein synthesis (EF-tu). The correlation of differential protein and mRNA expression varied between good and no correlation. Overall, these four proteins may be involved in the adaptation and survival of M. avium within human macrophages.
Brunori
Lara
L
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Roma, Italy.
Giannoni
Federico
F
Bini
Luca
L
Liberatori
Sabrina
S
Frota
Cristiane
C
Jenner
Peter
P
Thoresen
Ove Fredrik
OF
Orefici
Graziella
G
Fattorini
Lanfranco
L
D001426:Bacterial Proteins / Q000737:chemistry*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D009164:Mycobacterium Infections / Q000378:metabolism; D009162:Mycobacterium avium / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D010444:Peptide Elongation Factor Tu / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D010444:Peptide Elongation Factor Tu