Identification and determination of effective components in Euphrasia regelii by capillary zone electrophoresis.
A capillary zone electrophoresis (CZE) method has been developed for simultaneous determination of eukovoside, cinnamic acid and ferulic acid in Euphrasia regelii for the first time. The electrophoresis buffer was 20 mmol/L sodium borate containing 10% (v/v) methanol (pH 8.50). The effects of concentration of borate and electrolyte pH on electrophoretic behavior and separation were studied. Regression equations revealed linear relationships (correlation coefficients 0.9995-0.9998) between the peak area of each analyte and the concentration. The levels of analytes in the different parts of Euphrasia regelii were easily determined with recoveries ranging from 95.5 to 104.2%.
Shuya
Cui
C
Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
Shengda
Qi
Q
Xingguo
Chen
C
Zhide
Hu
H
D001881:Borates; D002021:Buffers; D002934:Cinnamates / Q000032:analysis*; D003373:Coumaric Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D032201:Euphrasia / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results
D001881:Borates; D002021:Buffers; D002934:Cinnamates; D003373:Coumaric Acids; D006027:Glycosides; D010636:Phenols; C488309:eukovoside; C029010:cinnamic acid; C004999:ferulic acid; D000432:Methanol