Use of sodA sequencing for the identification of clinical isolates of coagulase-negative staphylococci.
This study evaluated the possible advantages provided by a genotypic method over commercially available biochemical systems for the identification of clinical isolates of coagulase-negative staphylococci (CNS). Partial sequencing of the sodA gene was performed for 168 coagulase-negative clinical isolates of staphylococci identified previously with the ID32 STAPH system. Of these, 101 (60.1%) were identified to the species level with ID32 STAPH, while 67 (39.9%) were misidentified or not identified with certainty. Sequencing of sodA proved useful for resolving all ambiguities or inconclusive identifications generated by the commercially available biochemical identification system.
Sivadon
V
V
Laboratoire de Microbiologie, Hôpital Raymond Poincaré (AP-HP), Garches, France.
Rottman
M
M
Quincampoix
J-C
JC
Avettand
V
V
Chaverot
S
S
de Mazancourt
P
P
Trieu-Cuot
P
P
Gaillard
J-L
JL
D001426:Bacterial Proteins / Q000235:genetics*; D003428:Cross Infection / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021901:DNA, Intergenic; D011933:Reagent Kits, Diagnostic; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase