title: Effects of modified sample collection technique on fungal culture yield: nail clipping/scraping versus microdrill.
abstract: OBJECTIVE
  Onychomycosis requires accurate diagnosis but fungal culture yield is frequently low by routine sampling techniques. The aim of this study was to investigate the utility of nail plate/subungual microdrilling as an alternative to conventional nail clipping/subungual scraping.
  
  
  METHODS
  Patients with clinical evidence of onychomycosis (n=46) were prospectively evaluated for fungal potassium hydroride (KOH) microscopy and culture comparing two sampling techniques: nail clipping versus microdrilling.
  
  
  RESULTS
  Fungal cultures were positive in 48% with 2 additional cases detected by combining both methods. KOH microscopy was positive in 17% cases. Specimen obtained via the microdrill technique gave consistent heavier fungal growth on culture media. Candida species were the most common isolates (82.7% of cases) and were negative on KOH microscopy in 95 % of culture proven cases. The microdrill technique yielded consistent heavier growth on culture media
  
  
  CONCLUSIONS
  Microdrill technique improves laboratory diagnosis and ultimately treatment of onychomycosis, particularly in patients with repeated KOH microscopy and culture failure despite strong clinical suspicion.
authors.affiliation: Department of Pathology, Henry Ford Hospital, Detroit, Michigan, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Qureshi
authors.forename: H A
authors.initials: HA
authors.lastname: Ormsby
authors.forename: N
authors.initials: N
authors.lastname: Kapadia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003883:Arthrodermataceae / Q000254:growth & development / Q000302:isolation & purification; D001230:Aspergillus / Q000254:growth & development / Q000302:isolation & purification; D002175:Candida / Q000254:growth & development / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial / Q000379:methods; D005260:Female; D005658:Fungi / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D014009:Onychomycosis / Q000382:microbiology*; D010154:Pakistan; D011446:Prospective Studies; D014249:Trichophyton / Q000254:growth & development / Q000302:isolation & purification