Distribution of divalent metal transporter-1 in the monkey basal ganglia.
An accumulation of iron occurs in the brain with age, and it is thought that this may contribute to the pathology of certain neurodegenerative diseases, including Parkinson's disease. In this study, we elucidated the distribution of divalent metal transporter-1 (DMT1) in the monkey basal ganglia by immunocytochemistry, and compared it with the distribution of ferrous iron in these nuclei by Turnbull's Blue histochemical staining. We observed a general correlation between levels of DMT1, and iron staining. Thus, regions such as the caudate nucleus, putamen, and substantia nigra pars reticulata contained dense staining of DMT1 in astrocytic processes, and were also observed to contain large numbers of ferrous iron granules. The exceptions were the globus pallidus externa and interna, which contained light DMT1 staining, but large numbers of ferrous iron granules. The thalamus, subthalamic nucleus, and substantia nigra pars compacta contained neurons that were lightly stained for DMT1, but few or no iron granules. The high levels of DMT1 expression in some of the nuclei of the basal ganglia, particularly the caudate nucleus, putamen, and substantia nigra pars reticulata, may account for the high levels of iron in these regions.
Huang
E
E
Department of Anatomy, National University of Singapore, Singapore 119260.
Ong
W Y
WY
Connor
J R
JR
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001479:Basal Ganglia / Q000378:metabolism* / Q000503:physiopathology / Q000648:ultrastructure; D001480:Basal Ganglia Diseases / Q000378:metabolism / Q000503:physiopathology; D001812:Blood-Brain Barrier / Q000502:physiology; D027682:Cation Transport Proteins / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D005260:Female; D005295:Ferrocyanides; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007501:Iron / Q000378:metabolism*; D019189:Iron Metabolism Disorders / Q000378:metabolism / Q000503:physiopathology; D033862:Iron-Binding Proteins / Q000378:metabolism*; D008252:Macaca fascicularis; D008297:Male; D046529:Microscopy, Electron, Transmission; D011699:Putamen / Q000378:metabolism; D013378:Substantia Nigra / Q000378:metabolism; D000099236:Solute Carrier Family 11, Member 2
D027682:Cation Transport Proteins; D005295:Ferrocyanides; D007501:Iron; D033862:Iron-Binding Proteins; D000099236:Solute Carrier Family 11, Member 2; C042827:potassium iron(III) hexacyanoferrate (II)