Temporary stabilization of electron on quinone acceptor side of reaction centers from the bacterium Rhodobacter sphaeroides wild type and mutant SA(L223) depending on duration of light activation.
The dark reduction of photooxidized bacteriochlorophyll (P+) by photoreduced secondary quinone acceptor (QB-) in isolated reaction centers (RC) from the bacterium Rhodobacter sphaeroides wild type and mutant strain SA(L223) depending on the duration of light activation of RC was studied. The kinetics of the dark reduction of P+ decreased with increasing light duration, which is probably due to conformational changes occurring under prolonged light activation in RC from the wild type bacterium. In RC from bacteria of the mutant strain in which protonatable amino acid Ser L223 near QB is substituted by Ala, the dependence of reduction kinetics of P+ on duration of light was not observed. Such dependence, however, became observable after addition of cryoprotectors, namely glycerol and dimethylsulfoxide, to the RC samples from the mutant strain. It was concluded that substitution of Ser L223 with Ala disturbs the native mechanism of electrostatic stabilization of the electron in the RC quinone acceptor site. At the same time, an additional modification of RC hydrogen bonds by glycerol and dimethylsulfoxide probably includes various possibilities for more effective time delay of the electron on QB.
Knox
P P
PP
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia. knox@biophys.msu.ru.
Zakharova
N I
NI
Seifullina
N H
NH
Churbanova
I Yu
IY
Mamedov
M D
MD
Semenov
A Yu
AY
D001429:Bacteriochlorophylls / Q000378:metabolism; D016227:Benzoquinones / Q000378:metabolism*; D001665:Binding Sites; D004583:Electrons; D007700:Kinetics; D008027:Light; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction / Q000528:radiation effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D013997:Time Factors
D001429:Bacteriochlorophylls; D016227:Benzoquinones; D045322:Photosynthetic Reaction Center Complex Proteins; C004532:quinone