title: What can we learn from noncoding regions of similarity between genomes?
abstract: BACKGROUND
  In addition to known protein-coding genes, large amounts of apparently non-coding sequence are conserved between the human and mouse genomes. It seems reasonable to assume that these conserved regions are more likely to contain functional elements than less-conserved portions of the genome.
  
  
  METHODS
  Here we used a motif-oriented machine learning method based on the Relevance Vector Machine algorithm to extract the strongest signal from a set of non-coding conserved sequences.
  
  
  RESULTS
  We successfully fitted models to reflect the non-coding sequences, and showed that the results were quite consistent for repeated training runs. Using the learned models to scan genomic sequence, we found that they often made predictions close to the start of annotated genes. We compared this method with other published promoter-prediction systems, and showed that the set of promoters which are detected by this method is substantially similar to that detected by existing methods.
  
  
  CONCLUSIONS
  The results presented here indicate that the promoter signal is the strongest single motif-based signal in the non-coding functional fraction of the genome. They also lend support to the belief that there exists a substantial subset of promoter regions which share several common features including, but not restricted to, a relative abundance of CpG dinucleotides. This subset is detectable by a variety of distinct computational methods.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. td2@sanger.ac.uk
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Down
authors.forename: Tim J P
authors.initials: TJ
authors.lastname: Hubbard
mesh_terms: D000818:Animals; D001185:Artificial Intelligence; D001482:Base Composition / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data; D018899:CpG Islands / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics*; D016678:Genome; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008957:Models, Genetic; D011237:Predictive Value of Tests; D016415:Sequence Alignment / Q000379:methods*
chemical_list: D021901:DNA, Intergenic