Determination of fungicide residues in fruits by coupled-column liquid chromatography.
Coupled-column liquid chromatography with fluorescence detection was applied to the determination of o-phenylphenol and bitertanol residues in orange and banana fruits. After extraction with a mixture of acetone, dichloromethane-petroleum ether, and ethyl acetate, an extract aliquot of 100 microL was injected directly without any additional clean-up into the chromatographic system using two reversed phase C18 coupled columns. The LC-LC approach allowed automated sample clean up of the vegetal extracts, leading to a simple and rapid analytical procedure, with limits of quantification between 0.01 and 0.05 mg kg(-1). Recovery experiments performed on orange and banana samples fortified at different concentrations (0.01 - 4 mg kg(-1)) gave average recoveries between 70 and 113% with relative standard deviations lower than 15%. The procedure developed was finally applied to orange and banana samples from different geographical locations and the results were confirmed by GC-MS.
Zamora
Tatiana
T
Analytical Chemistry, Experimental Sciences Dept., University Jaume I, P.O. Box 8029 AP, Castellón, Spain.
Hidalgo
Carmen
C
López
Francisco J
FJ
Hernández
Félix
F
D001713:Biphenyl Compounds / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D015394:Molecular Structure; D028521:Musa / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000032:analysis*
D001713:Biphenyl Compounds; D005659:Fungicides, Industrial; D010573:Pesticide Residues; D014230:Triazoles; C004369:2-phenylphenol; C083642:bitertanol