Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: the use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction.
Simultaneous comparison of differentially expressed protein profiles of Prorocentrum triestinum grown under different growth phases and growth conditions indicated the presence of phase-specific and stress-responsive proteins, respectively. Correlation studies on these proteins in relation to cell division phasing patterns and to models of phytoplankton growth inferred the possible functions. Most notable among these proteins were groups of proteins thought to trigger or mediate cells through specific phases of division of this alga, e.g., BP1, BP2, PB1, PB2, and PB3. Other proteins (e.g., group 1 proteins) thought to be responsible for maintaining and supporting cell concentration under adverse conditions were found. Furthermore, another group of proteins (group 2 proteins) thought to be stress-responsive were also detected. Taken overall, these differentially expressed proteins provided important information for uncovering various protective and adaptive mechanisms in the dinoflagellate's life cycle. These proteins have the potential to serve as "indicator proteins" for rapid assessment of the nutritional or metabolic status of these phytoplankton cells,and monitoring the differential expression of these phase-specific proteins and stress-specific proteins could be an important biomarker for bloom prediction.
Chan
Leo Lai
LL
Department of Ecology and Biodiversity, The University of Hong Kong, HKSAR, PR China.
Hodgkiss
Ivor John
IJ
Wan
Jennifer Man-Fan
JM
Lum
John Hon-Kei
JH
Mak
Abby Sin-Chi
AS
Sit
Wai-Hung
WH
Lo
Samuel Chun-Lap
SC
D020418:Algal Proteins / Q000737:chemistry*; D002453:Cell Cycle; D015180:Electrophoresis, Gel, Two-Dimensional; D056890:Eukaryota / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D020418:Algal Proteins; D020543:Proteome