Are iridoids in leaf beetle larvae synthesized de novo or derived from plant precursors? A methodological approach.
Iridoids, belonging to a group of cyclopentanoid monoterpenoids, are secreted by many species of leaf beetles as a defense against predators. Using chemically modified precursors of iridoid biosynthesis, it has been shown that some leaf beetle larvae can synthesize these iridoids de novo as well as sequester plant-produced molecules. Stable isotope techniques can provide useful methods for studying terpenoid biosynthesis without disturbing the natural conditions much. Two terpenoid biosynthesis pathways (mevalonic acid (MVA) pathway and methylerythritol-4-phosphate (MEP) pathway) may lead to different delta13C signatures of the products. Our results from natural abundance 13C and 13C-labelled iridoid precursors in Gastrophysa viridula and Phaedon cochleariae suggested that the two leaf beetle species use only de novo synthesis of their defensive iridoids. We observed that the isotope signature of the leaf-beetle-produced iridoids (via the MVA pathway) resembled that of the MEP-derived monoterpenoids from plants. Owing to this close similarity in the natural 13C abundances in the plant and insect compounds, a determination of iridoid-origin in leaf beetle secretion may only be possible by use of isotopically labelled compounds.
Søe
Astrid R B
AR
Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Jena, Germany. asoe@ice.mpg.de
Bartram
Stefan
S
Gatto
Nathalie
N
Boland
Wilhelm
W
D000818:Animals; D001517:Coleoptera; D004896:Erythritol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D039823:Iridoids / Q000737:chemistry / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007814:Larva / Q000737:chemistry / Q000378:metabolism*; D008798:Mevalonic Acid / Q000737:chemistry / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D013403:Sugar Phosphates / Q000737:chemistry / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014835:Volatilization
C114232:2-C-methylerythritol 4-phosphate; D039823:Iridoids; D013403:Sugar Phosphates; D013729:Terpenes; D004896:Erythritol; D008798:Mevalonic Acid