title: Detection of zinc ions by differential circularly polarized fluorescence excitation.
abstract: A new chiroptical spectroscopic approach, differential circularly polarized fluorescence excitation (CPE), can be used to provide a selective method for detecting the presence of zinc ions. The approach utilizes the same instrumentation as fluorescence-detected circular dichroism and provides strong contrast in metal detection due to response to both chelation-enhanced fluorescence and circular dichroism upon metal ion binding. The observed contrast is therefore better than either of the parent spectroscopic detection methods. CPE also provides a strategy to reduce interference from background such as protein-based tryptophan fluorescence.
authors.affiliation: Department of Chemistry, New York University, New York, New York 10003, USA.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Dai
authors.forename: Gloria
authors.initials: G
authors.lastname: Proni
authors.forename: Danny
authors.initials: D
authors.lastname: Mancheno
authors.forename: Sasan
authors.initials: S
authors.lastname: Karimi
authors.forename: Nina
authors.initials: N
authors.lastname: Berova
authors.forename: James W
authors.initials: JW
authors.lastname: Canary
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002413:Cations, Divalent; D002645:Chickens; D002942:Circular Dichroism / Q000379:methods; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry; D005454:Fluorescence Polarization / Q000379:methods*; D009113:Muramidase / Q000032:analysis / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis / Q000737:chemistry; D015032:Zinc / Q000032:analysis*
chemical_list: D002413:Cations, Divalent; D004527:Egg Proteins; D014364:Tryptophan; D009113:Muramidase; D015032:Zinc