Exposure to work-related levels of swine dust up-regulates CD106 on human alveolar macrophages.
BACKGROUND
Exposure to dust from swine confinement buildings, causes an inflammatory response. Monocyte recruitment to the murine lung after instillation of lipopolysaccharide (LPS) has been shown to partially depend on CD106/VCAM1. We wanted to determine whether this can be confirmed in man.


METHODS
Non-naïve persons bearing personal air samples were exposed for 3 hr in a swine confinement building, and were bronchoscopied before and after exposure. Blood samples were taken at the time of bronchoscopy. Expression of adhesion molecules on leukocytes was investigated by flow cytometry in bronchoaveloar lavage (BAL) and blood.


RESULTS
Expression of CD106 on alveolar macrophages, but not on neutrophils or T cells in BAL or blood, was up-regulated in proportion to the amount of LPS that individuals were exposed.


CONCLUSIONS
This argues for requirement of CD106 during inflammatory recruitment of monocytes to the human lung.
Hoffmann
Hans Jürgen
HJ
Department of Pulmonary Medicine, Aarhus University Hospital, Aarhus, Denmark. hansjuergen.hoffmann@get2net.dk
Iversen
Martin
M
Takai
Hisamitsu
H
Sigsgaard
Torben
T
Omland
Øyvind
Ø
Dahl
Ronald
R
D000395:Air Pollutants, Occupational / Q000378:metabolism*; D016902:Air Pollution, Indoor / Q000032:analysis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D020680:Bronchoscopes; D004391:Dust / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis; D006801:Humans; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism*; D013552:Swine; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*
D000395:Air Pollutants, Occupational; D004391:Dust; D004731:Endotoxins; D019010:Vascular Cell Adhesion Molecule-1