Mycobacterium canariasense sp. nov.
A novel rapidly growing, non-pigmented mycobacterium was isolated from blood samples obtained from 17 patients with febrile syndrome. Bacterial growth occurred at 30 and 37 degrees C on Löwenstein-Jensen medium and also on MacConkey agar without crystal violet. Strains contained alpha- and alpha'-mycolates in their cell wall. Sequence analysis of the hsp65 and 16S rRNA genes identified the isolates as rapidly growing mycobacteria. Sequences of both genes were unique within the mycobacteria. DNA-DNA hybridization showed that the isolates had less than 15 % reassociation with 13 other recognized rapidly growing mycobacteria. The name Mycobacterium canariasense sp. nov. is proposed for this novel opportunistic pathogen, which is most closely related to Mycobacterium diernhoferi. The type strain is 502329T (= CIP 107998T = CCUG 47953T).
Jiménez
M Soledad
MS
Laboratorio de Micobacterias, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Campos-Herrero
M Isolina
MI
Servicio de Microbiología, Hospital de Gran Canaria Dr Negrin, Las Palmas de Gran Canaria, Spain.
García
Diana
D
Servicio de Microbiología, Hospital de Gran Canaria Dr Negrin, Las Palmas de Gran Canaria, Spain.
Luquin
Marina
M
Unitat de Microbiología, Departament de Genetica i de Microbiología, Universitat Autónoma de Barcelona, Bellaterra, Barcelona, Spain.
Herrera
Laura
L
Laboratorio de Micobacterias, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
García
María J
MJ
Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D002473:Cell Wall / Q000737:chemistry; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D003470:Culture Media; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D009164:Mycobacterium Infections / Q000382:microbiology; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D013696:Temperature
D001426:Bacterial Proteins; D018834:Chaperonin 60; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009171:Mycolic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins