Determination of triptolide and triptonide in human plasma by high-performance liquid chromatography.
An isocratic high-performance liquid chromatographic method for determination of triptolide and triptonide in human plasma is described. Plasma samples were extracted with OasisHLB solid-phase extraction (SPE) cartridges. After pretreatment, they were separated on a SymmetryShieldRP(18) column with a mobile phase of acetonitrile-water (40:60,v/v) at 40 degrees C. The effluent was monitored at UV 217 nm. Linearity (0.010-1.0 mg/L) was good, and the lower limit of detection was 3 ng/mL for triptolide and 4.5 ng/mL for triptonide (S/N = 3). The relative standard deviations of intra- and inter-day assay were less than 15% and the recoveries were better than 80%. The developed method was applied to the determination of triptolide and triptonide concentration in a patient's plasma after taking the medicament containing Tripterygium wilfordii Hook. F.
Wang
Zhaohong
Z
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Road 38, Beijing 100083, People's Republic of China.
Wen
Jiao
J
Xing
Junbo
J
Guo
Dean
D
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000097:blood*; D004852:Epoxy Compounds; D005260:Female; D006801:Humans; D010616:Phenanthrenes / Q000097:blood*; D010936:Plant Extracts / Q000097:blood; D015203:Reproducibility of Results; D014315:Triterpenes / Q000097:blood*
D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D010936:Plant Extracts; D014315:Triterpenes; C041579:lei gong teng; C001899:triptolide; C084079:triptonide