[Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes].
OBJECTIVE
To observe the immunosuppression effect of a novel HLA-derived peptide RDP1258, and to explore its mechanisms.


METHODS
RDP1258 was synthesized chemically. The effect of the peptide on alloreactive proliferation of rat splenocytes was observed by (3)H-TdR incorporation method. The heme oxygenase-1(HO-1) activity was analyzed by the enzyme assay.


RESULTS
The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of rat splenocytes and HO-1 activity in a dose-dependent manner in-vitro.


CONCLUSIONS
HO-1 may participate in the inhibitory effect of RDP1258 on the proliferation of rat splenocytes induced by mitogen or alloantigen.
Yi
Shan-hong
SH
Department of Urology, Xingqiao Hospital, Third Military Medical University, Chongqing, 400037, China. yshh881@sohu.com
Song
Bo
B
Wang
Ze-hou
ZH
Zhang
Gen-fu
GF
Cui
Zhi-gang
ZG
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology*
D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; C122045:RDP 1258; D051547:Heme Oxygenase-1