Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus).
Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on days 1, 3 and 7 or days 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on day 1. All tissues expressed a GLUT isoform on day 7 except for the thymus. Most tissues expressing a CAT isoform on day 1 decreased mRNA levels through day 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until day 7. The liver was the only tissue expressing GLUT-2 mRNA through day 14. On day 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High-affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on day 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.
Humphrey
Brooke D
BD
Department of Animal Science, University of California, 1 Shields Avenue, Davis, CA 95616, USA.
Stephensen
Charles B
CB
Calvert
Chris C
CC
Klasing
Kirk C
KC
D000375:Aging / Q000235:genetics; D026923:Amino Acid Transport Systems, Basic / Q000235:genetics*; D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D026923:Amino Acid Transport Systems, Basic; D009004:Monosaccharide Transport Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger