title: Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris.
abstract: The galactose-binding lectin EW29 from the earthworm Lumbricus terrestris is composed of two homologous domains, both of which are members of the R-type lectin family. The truncated mutant rC-half comprising the C-terminal domain was crystallized by the hanging-drop vapour-diffusion method. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 61.2, c = 175.6 A, and diffracted to beyond 1.9 A resolution. Matthews coefficient calculations suggested that this crystal contained two molecules per asymmetric unit.
authors.affiliation: Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Suzuki
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kasai
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods; D005690:Galactose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D009835:Oligochaeta / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012984:Software; D014961:X-Ray Diffraction
chemical_list: D037102:Lectins; D005690:Galactose