title: 14-3-3 regulation of cell spreading and migration requires a functional amphipathic groove.
abstract: The 14-3-3 proteins associate with many cellular proteins that participate in the regulation of various cellular events including apoptosis, the cell cycle, spreading, and migration. We have previously described that 14-3-3beta binds the beta1-integrin and overexpression of 14-3-3beta promoted increased cell spreading and migration (Han et al. [2001] Oncogene 20: 346-357). In this study, we find that mutation of Ser 60 of 14-3-3beta, outside of the amphipathic groove which is involved in 14-3-3 protein interactions with other ligands, abolished its interaction with integrin. Surprisingly, this mutant retained its ability to promote cell spreading, suggesting that 14-3-3beta interaction with the beta1-integrin is not required for its regulation of cell adhesion. We next showed that mutations of several critical residues in the amphipathic groove did not affect 14-3-3beta interaction with the beta1-integrin. As expected, these mutants disrupted their association with the phosphoserine dependent ligands Raf and Cas. Analysis of the groove mutant LF (mutation of Arg129Tyr130 to Leu and Phe) indicated that, unlike wild type 14-3-3beta, it could not stimulate cell spreading or migration, suggesting that a functional amphipathic groove is required for 14-3-3 regulation of cell adhesion and migration. Consistent with this, cells expressing the LF mutant exhibited a delay in F-actin organization compared to cells expressing wild type or the S60A mutant (Ser 60 to Ala mutation) upon cell adhesion to fibronectin (FN). Taken together, these studies identified a novel binding site on 14-3-3 for integrin beta1 and showed that a functional amphipathic groove, rather than its interaction with integrin beta1, is required for 14-3-3 regulation of cell spreading and migration.
authors.affiliation: Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Rodriguez
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: Guan
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D048430:Cell Shape / Q000235:genetics; D050719:Crk-Associated Substrate Protein; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism; D050717:Retinoblastoma-Like Protein p130; D012694:Serine / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D048948:14-3-3 Proteins; D000199:Actins; C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; D019012:Integrin beta1; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; C482982:YWHAB protein, human; D012694:Serine; D019908:Proto-Oncogene Proteins c-raf