title: Glucose transport in lactation.
abstract: Lactose is the major carbohydrate of human milk and is also the major osmotic constituent of human milk. Therefore, synthesis of lactose is the major determinant of the volume of milk produced by the lactating human mammary gland. Lactose issynthesized from free glucose and UDP-galactose. Thus, glucose transport is required not only across the plasma membrane but also across an intracellular membrane to the compartment of lactose synthesis. The latter requirement is unique to mammary epithelial cells. Historically, based primarily on subcellular fractionation studies, lactose synthesis was thought to occur in Golgi. Yet, the only known glucose transporter isoform expressed in mammary gland is GLUT1, a plasma membrane glucose transporter. We therefore comprehensively studied the possible role of GLUT1 as a glucose transporter. We tested the hypothesis that changes in the amount, activity, and subcellular targeting of GLUT1 during lactation are consistent with an important role for GLUT1 in the regulation of lactation. The experiments described here summarize our recent work in the lactating mouse mammary gland and inmouse mammary epithelial cells in culture. Theresults demonstrate that GLUT1 is targeted to an intracellular compartment. However, studies in mammary epithelial cells in culture demonstrate that this is not a Golgi compartment, but a low-density, exquisitely Brefeldin A-sensitive compartment of Golgi-related vesicles. This raises the possibility that lactose synthesis does not take place in the Golgiproper. The results strongly suggest that GLUT1 appears to be important in delivery of substrate to the site of lactose synthesis.
authors.affiliation: Department of Pediatrics, Section of Neonatology, USDA/ARS, Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030, USA. phaney@neo.bcm.tmc.edu
authors.forename: Peter M
authors.initials: PM
authors.lastname: Haney
mesh_terms: D000818:Animals; D020126:Brefeldin A / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D051272:Glucose Transporter Type 1; D006056:Golgi Apparatus / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008892:Milk / Q000737:chemistry; D008895:Milk, Human / Q000737:chemistry; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495678:Slc2a1 protein, mouse; D020126:Brefeldin A; D007785:Lactose