Effect of prostaglandins against alloxan-induced cytotoxicity to insulin secreting insulinoma RIN cells in vitro.
In the present study, we studied the effect of various prostaglandins (PGs) on alloxan-induced cytotoxicity to rat insulinoma (RIN) cells. Of all the PGs tested, PGE(1), PGE(2), PGI(2), PGF(1 alpha), and PGF(3 alpha) protected RIN cells from alloxan-induced cytotoxicity (P<0.05 compared to alloxan), whereas thromboxane B(2) and 6-keto-PGF(1 alpha) were not effective. PGE(1) induces a statistically significant increase in the activities of superoxide dismutase and glutathione peroxidase and decrease in lipid peroxides in alloxan-treated RIN cells (P<0.001). PGE(1) restored nitric oxide/lipid peroxide ratio to normalcy, suggesting that PGE(1) suppresses oxidant stress induced by alloxan in RIN cells in vitro. Furthermore, PGE(1) prevented DNA damage and apoptosis induced by alloxan. These results indicate that PGE(1) prevents alloxan-induced cytotoxicity to RIN cells in vitro.
Devi
M M Sailaja
MM
UND Life Sciences, 1083 Main Street, Walpole, MA 02081, USA.
Das
Undurti Narasimha
UN
D015121:6-Ketoprostaglandin F1 alpha / Q000494:pharmacology; D000496:Alloxan / Q000494:pharmacology*; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology; D011464:Epoprostenol / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010190:Pancreatic Neoplasms; D011453:Prostaglandins / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D013929:Thromboxane B2 / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D007328:Insulin; D008054:Lipid Peroxides; D011453:Prostaglandins; D011460:Prostaglandins F; D009599:Nitroprusside; D009569:Nitric Oxide; D008315:Malondialdehyde; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003994:Bucladesine; D000496:Alloxan; C034238:prostaglandin F3alpha; D011464:Epoprostenol; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000527:Alprostadil; D015232:Dinoprostone; C100006:prostaglandin F1