Immunocytochemical demonstration of down regulation of HLA class-I molecule expression in human metastatic breast carcinoma.
Deficient expression of HLA class-I molecules observed in many cancers is suggested to influence both disease progression and potential efficacy of T cell-mediated immune therapy. Previous studies have attempted to correlate either primary breast cancer tumor grade with HLA class-I levels or the presence of HLA class-I-deficient cells in metastatic lesions with survival. In this study we evaluated the HLA class-I status of matched primary and secondary breast cancer lesions in order to ask the question: is metastasis of breast cancer associated with down-regulation of HLA class-I expression? Immunocytochemistry analysis shows a definitive correlation between diminished HLA class-I expression and dissemination of breast cancer to tumor-draining lymph nodes: both the total number of HLA class-I+ cells per sample and the levels of expression are dramatically decreased in secondary versus primary tumor lesions. These findings are consistent with the contention that the ability of breast cancer cells to escape the confines of the original tumor lesion requires down-regulation of HLA class-I expression and implies that enhancing HLA class-I expression in secondary breast cancer may have a beneficial effect on T-cell-mediated immunotherapy.
Saio
Masanao
M
Department of Cellular Pathology, Division of Bioregulatory Medicine, Gifu University School of Medicine, Gifu, Japan.
Teicher
Matt
M
Campbell
Gaynor
G
Feiner
Helen
H
Delgado
Yara
Y
Frey
Alan B
AB
D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D015536:Down-Regulation; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D009362:Neoplasm Metastasis / Q000276:immunology*
D015395:Histocompatibility Antigens Class I