Chimeric LNA/DNA probes as a detection system for real-time PCR.
OBJECTIVE
Evaluation of the use of short probes as a detection system in real-time PCR.


METHODS
Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum.


RESULTS
The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal.


CONCLUSIONS
Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
Costa
Jean-Marc
JM
M. Dassault Molecular Biology Laboratory, Centre de Diagnostic Prénatal, American Hospital of Paris, Neuilly, 92200 Neuilly-sur-Seine, France. jean-marc.costa@ahparis.org
Ernault
Pauline
P
Olivi
Martine
M
Gaillon
Thierry
T
Arar
Khalil
K
D041322:Chromosomes, Human, Y; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics; D010949:Plasma / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors / Q000235:genetics*
D015342:DNA Probes; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; C477371:locked nucleic acid