A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes.
BACKGROUND
The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5.


RESULTS
A total of 169 markers (21 microsatellites and 148 ESTs) were typed on the ChickRH6 RH panel, of which 134 were assigned to GGA5. The final map is composed of 73 framework markers extending over a 1315.6 cR distance. The remaining 61 markers were placed alongside the framework markers within confidence intervals.


CONCLUSIONS
The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. Only two discrepancies were observed in relation to the sequence assembly recently reported for this chromosome.
Pitel
Frédérique
F
Laboratoire de Génétique Cellulaire, INRA, Castanet-Tolosan, 31326, France. pitel@toulouse.inra.fr.
Abasht
Behnam
B
Morisson
Mireille
M
Crooijmans
Richard P M A
RP
Vignoles
Florence
F
Leroux
Sophie
S
Feve
Katia
K
Bardes
Suzanne
S
Milan
Denis
D
Lagarrigue
Sandrine
S
Groenen
Martien A M
MA
Douaire
Madeleine
M
Vignal
Alain
A
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D002877:Chromosomes, Human / Q000235:genetics*; D004247:DNA / Q000235:genetics; D020224:Expressed Sequence Tags; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D051379:Mice; D021101:Radiation Hybrid Mapping / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods
D005819:Genetic Markers; D004247:DNA