[Experimental study on the pharmacology of 999 ganmaoling, a compound recipe of Chinese and Western materia medica].
OBJECTIVE
To study the pharmacologic characteristics in synergism and complementation of 999 Ganmaoling (GML), a compound recipe composed of Chinese and Western materia medica (CMM & WMM), as well as its theoretical basis of matching of Chinese and Western materia medica.


METHODS
The torsion response induced by glacial acetic acid in mice, toe swelling induced by carrageenanin rats, delayed hypersensitive response in mice and fever induced by endotoxin in rats and rabbits were used to comparatively study the actions of CMM & WMM in GML. The effect of CMM in antagonizing liver damage caused by WD (acetaminophen) in mice was also studied. RT-PCR method was used to analyze the expression of related cytokines.


RESULTS
GML showed a significant antipyretic and analgesic effect, it could inhibit the carrageenan induced inflammation, antagonize the endotoxin induced fever, and promote the amount for expression of cytokines in rats' splenic tissue with pneumococci infection to some extent. The CMM in GML showed certain protective effect on acetaminophen induced liver damage.


CONCLUSIONS
GML has a potent antipyretic, analgesic and anti-inflammatory effects, CMM & WMM in GML showed markedly synergism and complementation, and CMM in it has liver protective effect.
Mao
Yi-qing
YQ
Faculty of Pharmacology, Health Science Center, Medical School, Peking University, Beijing (100083).
Mu
Zhi-xin
ZX
Zhang
Yue-fei
YF
D000082:Acetaminophen / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002744:Chlorpheniramine / Q000494:pharmacology; D003139:Common Cold / Q000188:drug therapy; D004338:Drug Combinations; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000082:Acetaminophen; D002110:Caffeine; D002744:Chlorpheniramine