Identification of hydroxyl radicals after UV irradiation of aqueous extracts of cigarette smoke and evaluation of urinary 8-OHdG in smokers.
The role of oxidative stress in tobacco smoke damage was examined using a combination of in vitro and in vivo approaches. Aqueous extracts of cigarette smoke were irradiated with UV and hydroxyl radical generation was evaluated by electron spin resonance (ESR). The spectra obtained revealed spin adducts of the hydroxyl radical (*OH) to increase with the volume of the aqueous extract of cigarette smoke, cigarette smoke collection flow, and UV irradiation time. Hydroxyl radical generation persisted for long hours, showing no change over time. Ascorbic acid protected against radical generation in a marked concentration-dependent fashion. In specimens of urine from volunteers, 8-OHdG levels and the 8-OHdG-production rate per hour were found to be higher in smokers than non-smokers. Our results suggest that more marked oxidative stress occurs with the smoking habit.
Yatsuzuka
Miki
M
Department of Adult Nursing (Acute Stage), Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Tazawa
Kenji
K
Yasuda
Tomomi
T
Yoshii
Miho
M
Ogawa
Kouhei
K
Wada
Shigeto
S
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000032:analysis*; D006801:Humans; D018384:Oxidative Stress; D012907:Smoking / Q000009:adverse effects*; D014466:Ultraviolet Rays
D000975:Antioxidants; D005609:Free Radicals; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D001205:Ascorbic Acid