Reduced plasma membrane Ca2+-ATPase function in platelets from patients with non-insulin-dependent diabetes mellitus.
We clearly show that plasma membrane Ca2+ ATPase (PMCA) activity is lower in platelets from patients with non-insulin-dependent diabetes mellitus (NIDDM) than in those from healthy controls. The lower activity is likely due to reduced PMCA expression and increased tyrosine phosphorylation. These findings provide an explanation for the cellular ionic defects occurring in insulin resistant conditions.
Rosado
Juan Antonio
JA
Saavedra
Francisco Raúl
FR
Redondo
Pedro Cosme
PC
Hernández-Cruz
Juan Manuel
JM
Salido
Ginés María
GM
Pariente
José Antonio
JA
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D002118:Calcium / Q000097:blood; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000737:chemistry / Q000172:deficiency*; D027682:Cation Transport Proteins / Q000097:blood / Q000737:chemistry / Q000172:deficiency*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000201:enzymology; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D015759:Ionomycin / Q000494:pharmacology; D008297:Male; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D053499:Plasma Membrane Calcium-Transporting ATPases; D015539:Platelet Activation; D011499:Protein Processing, Post-Translational; D019284:Thapsigargin / Q000494:pharmacology
D027682:Cation Transport Proteins; D019000:Phosphotyrosine; D015759:Ionomycin; D019284:Thapsigargin; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium