title: Dark-cell areas in the dog vestibular endorgans: an immunohistochemical study.
abstract: The stria vascularis in the cochlea and the dark-cell areas in the vestibular endorgans are structures involved in the production of endolymphatic fluid. This study investigated the dark-cell areas in the vestibular endorgans of the dog by classical staining and by immunohistochemistry (anti-Na,K-ATPase beta2 isoform, anti-cytokeratins (against cytokeratins 5 and 8), anti-vimentin and anti-S100A6) from birth to 110 postnatal days. Using classical staining, it was not possible to discriminate dark cells from other epithelial cells lining the vestibular endolymphatic spaces. From birth, the Na,K-ATPase beta2 isoform was expressed in the lateral and basal cell membranes of a subset of cells located in the utricular wall, at the base of the cristae ampullaris and was identified as dark cells. From birth, anti-cytokeratins labelled all the cells forming the epithelial lining, including the dark cells, while anti-vimentin labelled the underlying mesenchymal cells. From postnatal day 10, anti-S100A6 labelled subepithelial cells exclusively located underneath the dark-cell areas and were identified as vestibular melanocyte-like cells. From birth, Fontana staining evidenced fine melanin granules in the subepithelial layer. The amount of melanin granules increased during the first month. Melanin distribution was closely associated with the region where S100A6-positive cells were located. The cell-specific antigen expression in the dog dark-cell areas was clearly comparable to that of the dog stria vascularis previously described. The present investigation also suggested an earlier histological and immunohistological maturity in the dark-cell areas than in the stria vascularis of dogs. This preliminary morphological description of the normal dark-cell areas in dogs by means of immunomarkers may be instrumental in studying pathological processes involving the fluid-secreting structures in vestibular endorgans.
authors.affiliation: Laboratory of Anatomy and Embryology, Veterinary Anatomy, Free University of Brussels, Brussels, Belgium. angelique.coppens@ulb.ac.be
authors.forename: A G
authors.initials: AG
authors.lastname: Coppens
authors.forename: I
authors.initials: I
authors.lastname: Salmon
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: L
authors.initials: L
authors.lastname: Poncelet
mesh_terms: D000818:Animals; D004285:Dogs / Q000033:anatomy & histology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008297:Male; D009418:S100 Proteins / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013194:Staining and Labeling; D014722:Vestibule, Labyrinth / Q000166:cytology* / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
chemical_list: D009418:S100 Proteins; D014746:Vimentin; D007633:Keratins; D000254:Sodium-Potassium-Exchanging ATPase