title: The ClpP protease of Streptococcus pneumoniae modulates virulence gene expression and protects against fatal pneumococcal challenge.
abstract: Streptococcus pneumoniae usually colonizes the nasopharynx of humans asymptomatically but occasionally translocates from this niche to the lungs, the brain, and the blood, causing potentially fatal infections. Spread to other host tissues requires a significant morphological change and the expression of virulence factors, such as capsular polysaccharide, and virulence proteins, such as pneumolysin (Ply), PspA, and CbpA. Modulation of the expression of pneumococcal virulence genes by heat shock and by heat shock proteins ClpL and ClpP, as well as the attenuation of virulence of a clpP mutant in a murine intraperitoneal infection model, was demonstrated previously. In this study, we further investigated the underlying mechanism of virulence attenuation by the clpP mutation. The half-lives of the mRNAs of ply and of the first gene of the serotype 2 capsule synthesis locus [cps2A] in the clpP mutant were more than twofold longer than those of the parent after heat shock, suggesting that the mRNA species were regulated posttranscriptionally by ClpP. In addition, the clpP mutant was defective in colonization of the nasopharynx and survival in the lungs of mice after intranasal challenge. The mutant was also killed faster than the parent in the murine macrophage RAW264.7 cell line, indicating that ClpP is required for colonization and intracellular survival in the host. Furthermore, fractionation studies demonstrated that ClpP was translocated into the cell wall after heat shock, and immunization of mice with ClpP elicited a protective immune response against fatal systemic challenge with S. pneumoniae D39, making ClpP a potential vaccine candidate for pneumococcal disease.
authors.affiliation: College of Pharmacy, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Hyog-Young
authors.initials: HY
authors.lastname: Kwon
authors.forename: A David
authors.initials: AD
authors.lastname: Ogunniyi
authors.forename: Moo-Hyun
authors.initials: MH
authors.lastname: Choi
authors.forename: Suhk-Neung
authors.initials: SN
authors.lastname: Pyo
authors.forename: Dong-Kwon
authors.initials: DK
authors.lastname: Rhee
authors.forename: James C
authors.initials: JC
authors.lastname: Paton
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002473:Cell Wall / Q000201:enzymology; D049071:Endopeptidase Clp; D015964:Gene Expression Regulation, Bacterial; D006207:Half-Life; D018869:Heat-Shock Response; D006461:Hemolysis; D006801:Humans; D008168:Lung / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008808:Mice, Inbred CBA; D009154:Mutation / Q000235:genetics; D009305:Nasopharynx / Q000382:microbiology; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D015996:Survival Rate; D014774:Virulence / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases