title: Production of ribonucleotides by autolysis of Pichia anomala mutant and some physiological activities.
abstract: Various mutants of Pichia anomala were isolated by ethyl methanesulfonate (EMS) treatment and UV irradiation through cycloheximide resistance and KCl sensitivity. The selected mutant HA-2 accumulated a higher content of RNA and grew faster than the wild-type strain in yeast extract-malt (YM) broth. Autolysis of the HA-2 mutant at 60 degrees C and pH 7.0 for 6 h was the best condition to obtain maximum yields of 5'-ribonucleotides, inosinic monophosphate (IMP) (6.2 mg/g biomass) and guanylic monophosphate (GMP) (35.5 mg/g biomass). The yield of adenylic monophosphate (AMP) (7.8 mg/g biomass) was optimal at 60 degrees C at pH 6.5 for 6 h. The inhibitory activity of the angiotensin-converting enzyme and the nitrite-scavenging activity for autolysates of the HA-2 mutant were about 13.0% and 47.0% higher than those of native strain, respectively.
authors.affiliation: Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Daejon, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.forename: Kwang-Wook
authors.initials: KW
authors.lastname: Hyun
authors.forename: Seung-Chan
authors.initials: SC
authors.lastname: Jeong
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Young J
authors.initials: YJ
authors.lastname: Choi
authors.forename: Carlos B
authors.initials: CB
authors.lastname: Miguez
mesh_terms: D000249:Adenosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis; D000935:Antifungal Agents / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis; D006157:Guanosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007291:Inosine Monophosphate / Q000032:analysis / Q000096:biosynthesis; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D011189:Potassium Chloride / Q000494:pharmacology; D012265:Ribonucleotides / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000935:Antifungal Agents; D016166:Free Radical Scavengers; D009153:Mutagens; D012265:Ribonucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D011189:Potassium Chloride; D006157:Guanosine Monophosphate; D003513:Cycloheximide; D005020:Ethyl Methanesulfonate