title: Nontuberculous mycobacteria in hospital water systems: application of HPLC for identification of environmental mycobacteria.
abstract: Nontuberculous mycobacteria (NTM), ubiquitous in water environments, are increasingly recognized as nosocomial pathogens. Our study reports a one-year survey of the water system of two hospitals, A and B, in a small town near Florence, Italy. NTM were found throughout the study period in both settings, but B showed a significantly higher mycobacterial load. Mycobacterium gordonae and Mycobacterium fortuitum were the most frequent species isolated. Identification was carried out by conventional techniques and by high performance liquid chromatography (HPLC) analysis of cell wall mycolic acids. HPLC profiling could be used as a first-choice method for identification of environmental mycobacteria.
authors.affiliation: Dipartimento Sanità Pubblica, Università di Firenze, Viale GB Morgagni 48, I50134 Florence, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Galassi
authors.forename: R
authors.initials: R
authors.lastname: Donato
authors.forename: E
authors.initials: E
authors.lastname: Tortoli
authors.forename: D
authors.initials: D
authors.lastname: Burrini
authors.forename: D
authors.initials: D
authors.lastname: Santianni
authors.forename: R
authors.initials: R
authors.lastname: Dei
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003625:Data Collection; D004784:Environmental Monitoring; D006761:Hospitals; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D014881:Water Supply / Q000592:standards*