RNA-based gene transfer for adult stem cells and T cells.
Electroporation of mRNA has become an established method for gene transfer into dendritic cells for immunotherapeutic purposes. However, many more cell types and applications might benefit from an efficient mRNA-based gene transfer method. In this study, we investigated the potential of mRNA-based gene transfer to induce short-term transgene expression in adult stem cells and activated T cells, based on electroporation with mRNA encoding the enhanced green fluorescent protein. The results show efficient transgene expression in CD34-positive hematopoietic progenitor cells (35%), in in vitro cultured mesenchymal cells (90%) and in PHA-stimulated T cells (50%). Next to presentation of gene transfer results, potential applications of mRNA-based gene transfer in stem cells and T cells are discussed.
Smits
E
E
Laboratory of Experimental Hematology, Faculty of Medicine, Antwerp University Hospital, University of Antwerp, Edegem, Belgium.
Ponsaerts
P
P
Lenjou
M
M
Nijs
G
G
Van Bockstaele
D R
DR
Berneman
Z N
ZN
Van Tendeloo
V F I
VF
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D018274:Electroporation / Q000592:standards*; D018014:Gene Transfer Techniques / Q000592:standards*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000235:genetics; D006801:Humans; D008213:Lymphocyte Activation; D008648:Mesoderm / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism*; D019076:Transgenes / Q000502:physiology*
D018952:Antigens, CD34; D010835:Phytohemagglutinins; D012333:RNA, Messenger