A new VHR dual-specificity protein tyrosine phosphatase inhibitor from Dendrobium moniliforme.
Bioassay-guided fractionation of the EtOAc-soluble extract of Dendrobium moniliforme afforded a new phenanthraquinone-type metabolite, 7-hydroxy-5,6-dimethoxy-1,4-phenanthrenequinone ( 1), along with the previously reported 5-hydroxy-3,7-dimethoxy-1,4-phenanthrenequinone ( 2). The structures of the compounds were identified mainly on the basis of MS and NMR data. Compound 1 inhibited VHR dual-specificity protein tyrosine phosphatase (DS-PTPase) activity in a dose-dependent manner, displaying an IC (50) value of 3.0 +/- 0.2 microM.
Bae
Eun Young
EY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yusong, Taejeon 305-333, South Korea.
Oh
Hyuncheol
H
Oh
Won Keun
WK
Kim
Min Soo
MS
Kim
Beom Seok
BS
Kim
Bo Yeon
BY
Sohn
Cheon Bae
CB
Osada
Hiroyuki
H
Ahn
Jong Seog
JS
D031667:Dendrobium; D004305:Dose-Response Relationship, Drug; D054640:Dual Specificity Phosphatase 3; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018547:Plant Stems; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D010936:Plant Extracts; C517025:DUSP3 protein, human; D054640:Dual Specificity Phosphatase 3; D017027:Protein Tyrosine Phosphatases