A rapid antimicrobial susceptibility test based on chemiluminescence assay and its application to screening of genotypes in vancomycin-resistant enterococci.
Minimum inhibitory concentrations (MICs) of vancomycin (VCM) and teicoplanin (TEIC) were measured using a novel susceptibility test based on the chemiluminescence assay (CA) method (Rapid-Lumi Eiken; Eiken Chemicals, Tokyo, Japan) against 33 strains in total: 7, 5, and 10 strains of which are VCM-resistant enterococci (VRE) with vanA, vanB, and vanC genes, respectively, and the other 11 strains are vancomycin-susceptible enterococci (VSE). The results were in good accordance with the values determined by the standard broth dilution method approved by the National Committee for Clinical Laboratory Standards (NCCLS): i.e., 88% (29/33) of consistency for VCM and 97% (32/33) for TEIC, respectively. In addition, genotypes in VRE strains (vanA, vanB, vanC-1, and vanC-2/3 genes) were properly estimated from the results of the CA method and the NCCLS interpretive categories, even though the incubation time was very short (2-4 h). In conclusion, it was found that the new method is reliable and rapid to detect VRE strains in clinical laboratories.
Nagasawa
Zenzo
Z
Department of Microbiology and Immunology, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. nagasaz@post.saga-med.ac.jp
Manome
Isao
I
Nagayama
Ariaki
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D003470:Culture Media; D016983:Enterococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D017334:Teicoplanin / Q000494:pharmacology*; D013997:Time Factors; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D017334:Teicoplanin; D014640:Vancomycin