Application of micellar electrokinetic capillary chromatography to the analysis of uncharged pesticides of environmental impact.
A test mixture of five pesticides and metabolites (naphthalene acetamide, carbaryl, 1-naphthol, thiabendazole, and carbendazime) has been investigated by capillary electrophoresis with an ultraviolet diode array detector. These compounds were separated in <10 min by micellar electrokinetic capillary chromatography (MEKC). MEKC was performed in 30 mM ammonium chloride/ammonia buffer (pH 9.0) containing 15 mM sodium dodecyl sulfate. The lowest detection limit was obtained for the insecticide carbaryl (0.22 microg mL(-)(1)) and the highest for its metabolite 1-naphthol (1.13 microg mL(-)(1)). This method was applied to the analysis of the pesticides in cultivated vegetables such as cucumbers, which were extracted with a liquid-liquid extraction procedure, obtaining recovery percentages ranging from 90.1 to 110.2%.
Segura Carretero
Antonio
A
Department of Analytical Chemistry, Faculty of Science, University of Granada, Granada, Spain. ansegura@ugr.es
Cruces-Blanco
Carmen
C
Cortacero Ramírez
Sonia
S
Carrasco Pancorbo
Alegría
A
Fernández Gutiérrez
Alberto
A
D000383:Agriculture; D001562:Benzimidazoles / Q000032:analysis; D002219:Carbamates; D012721:Carbaryl / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D009281:Naphthalenes / Q000032:analysis; D009284:Naphthols / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D013827:Thiabendazole / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
D001562:Benzimidazoles; D002219:Carbamates; D004785:Environmental Pollutants; D009281:Naphthalenes; D009284:Naphthols; D010575:Pesticides; C031721:naphthalene; C029350:1-naphthol; C006698:carbendazim; D013827:Thiabendazole; D012721:Carbaryl