Spatial and temporal gene expression profiling of the contused rat spinal cord.
Microarray technology was used to examine gene expression changes following contusive injury of the adult rat spinal cord. To obtain a global understanding of the changes triggered by the injury, differential gene expression was examined spatially, using tissue samples from the epicenter of injury as well as 1 cm rostral and 1 cm caudal to the epicenter, and temporally, at 3 h, 24 h, 7 days, and 35 days post-injury. To filter out gene expression changes that were due to the laminectomy, samples of contused tissue were compared to laminectomy-only controls. We took advantage of four different, complementary methods of data analysis to detect differentially expressed genes. We have identified functional groups of genes that are differentially regulated in our model, including those associated with apoptosis, cell cycle, inflammation, and cholesterol metabolism. Our analysis has led to the identification of novel potential therapeutic targets within each group of genes that is discussed.
Aimone
James B
JB
The Christopher Reeve Paralysis Foundation Research Consortium, Springfield, NJ 07081, USA.
Leasure
J Leigh
JL
Perreau
Victoria M
VM
Thallmair
Michaela
M
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002403:Cathepsins / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D016172:DNA Fingerprinting; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018816:Genes, cdc / Q000502:physiology; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics; D010587:Phagocytosis / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D011930:Reaction Time / Q000235:genetics; D013116:Spinal Cord / Q000378:metabolism* / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D009435:Synaptic Transmission / Q000235:genetics; D013997:Time Factors
D002784:Cholesterol; D002403:Cathepsins