Oxidative stress: potential of distinct peroxide determination systems.
When reactive oxygen species attack biological structures, peroxides, which are short-lived oxidative intermediates, are generated. We evaluated the potential of two different, commercially available peroxide activity assays (Pox-Act and d-ROMS) to see whether the results were associated with the clinical condition of subjects who were participating in a routine health care program. Furthermore, we determined the total antioxidant status (TAS) and the titer of autoantibodies against oxidized low-density lipoprotein (oLAb) to verify the hydroperoxide measurements. Subjects with medical conditions (hereafter referred to as patients) had significantly increased serum peroxide levels compared to healthy subjects. The d-ROMS kit indicated that 86% of subjects had an increased level of total peroxides. Although the assays had a significant correlation (p<0.001), 34% of the subjects had an increased total peroxide concentration in the Pox-Act assay that was clearly associated with clinical symptoms. Furthermore, the sensitivity of the Pox-Act assay was 35 times higher than that of the d-ROMS kit. In subjects with medical conditions, there was a trend toward a decreased TAS and a slightly increased oLAb titer in comparison to healthy subjects, but this was not statistically significant. The Pox-Act assay seems to be a valuable tool for the determination of total peroxides, while the results from the d-ROMS kit should be considered with caution.
Lindschinger
Meinrad
M
Institute for Nutritional and Metabolic Diseases, Schwarzl Outpatient Clinic, Lassnitzhöhe, Austria. office@lindschinger.at
Nadlinger
Karl
K
Adelwöhrer
Norbert
N
Holweg
Karin
K
Wögerbauer
Markus
M
Birkmayer
Jörg
J
Smolle
Karl-Heinz
KH
Wonisch
Willibald
W
D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000032:analysis / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000975:Antioxidants; D001323:Autoantibodies; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein