Effect of diet and dietary fatty acids on the transformation and incorporation of C18 fatty acids in double-muscled Belgian Blue young bulls.
Three groups of double-muscled Belgian Blue young bulls were fed during different stages of production diets differing in the proportions of linolenic and linoleic acid by including linseed in the concentrate or giving grass silage as main linolenic acid suppliers. Samples of rumen and abomasal contents and of the longissimus thoracis, subcutaneous fat, and liver were taken to analyze the fatty acid pattern with emphasis on the individual trans (t) C18:1 fatty acids and cis-9,trans-11 conjugated linoleic acid (c9t11CLA). Trans C18:1 isomers represented up to 20 g/100 g of total fatty acids in rumen and abomasal contents, whereas the accumulation of c9t11CLA was limited. Total trans C18:1 content in subcutaneous fat and intramuscular fat of the longissimus thoracis comprised 8.4 and 5.2 g/100 g of total fatty acids, respectively, with t11C18:1 being the most abundant one. Compared to rumen contents, subcutaneous and intramuscular fat were enriched in c9t11CLA and contained fewer tC18:1 isomers, resulting in a higher c9t11CLA/t11C18:1 ratio (0.04, 0.22, and 0.22, respectively). This result suggests that the endogenous synthesis of c9t11CLA in adipose tissue by the Delta(9)-desaturase was more important than its ruminal production.
Raes
Katleen
K
Department of Animal Production, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium. Katleen.raes@UGent.be
Fievez
Veerle
V
Chow
Tsang Tsey
TT
Ansorena
Diana
D
Demeyer
Daniel
D
De Smet
Stefaan
S
D000018:Abomasum / Q000737:chemistry; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D004063:Digestion; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000032:analysis; D019787:Linoleic Acid / Q000008:administration & dosage; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis; D008043:Linseed Oil / Q000008:administration & dosage; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D012417:Rumen / Q000737:chemistry; D017962:alpha-Linolenic Acid / Q000008:administration & dosage
D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005232:Fatty Acids, Volatile; D044243:Linoleic Acids, Conjugated; D017962:alpha-Linolenic Acid; D008043:Linseed Oil; D019787:Linoleic Acid