title: Gene-transferring efficiencies of novel diamino cationic lipids with varied hydrocarbon chains.
abstract: Utilizing three biocompatible components, a series of novel cationic lipids has been chemically synthesized and tested for their gene-transferring capabilities in 293 transformed kidney cells and B16BL6 mouse melanoma cells. The synthesized cationic lipids consisting of a core of lysine and aspartic acid with hydrocarbon chains of varied length were assigned the acronyms DLKD (O,O'-dilauryl N-lysylaspartate), DMKD (O,O'-dimyristyl N-lysylaspartate), DPKD (O,O'-dipalmityl N-lysylaspartate), and DSKD (O,O'-distearyl N-lysylaspartate). The gene-transferring capabilities of these cationic lipids were found to be dependent on the hydrocarbon chain length. Under similar experimental conditions, the order of gene transfection efficiency was DMKD > DLKD > DPKD > DSKD. Addition of cholesterol or dioleoyl phosphatidylethanolamine (DOPE) as a colipid did not change this order. Colipid addition affected the transfection efficiency positively or negatively depending on the length of the cationic lipid acyl chain. On the whole, the length of the hydrophobic carbon chain was a major factor governing the gene-transferring capabilities of this series of cationic lipids. The observed differences in transfection efficiency may be due to differing binding affinities to DNA molecules as well as differences in the surface charge potential of the liposome-DNA complexes (lipoplexes) in the aqueous environment.
authors.affiliation: Department of Biomedical Laboratory Science, Yonsei University, Wonju 220-710, Korea.
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Jaeho
authors.initials: J
authors.lastname: Moon
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Kim
authors.forename: Myung Min
authors.initials: MM
authors.lastname: Choi
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Yeon
authors.initials: Y
authors.lastname: Heo
authors.forename: Dae Hyan
authors.initials: DH
authors.lastname: Cho
authors.forename: Doo Ok
authors.initials: DO
authors.lastname: Jang
authors.forename: Yong Serk
authors.initials: YS
authors.lastname: Park
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D018014:Gene Transfer Techniques; D006801:Humans; D006838:Hydrocarbons / Q000008:administration & dosage*; D008081:Liposomes / Q000008:administration & dosage*; D051379:Mice; D014162:Transfection
chemical_list: D006838:Hydrocarbons; D008081:Liposomes