title: [Immune response of suckling mouse brain (CRL) rabies vaccine and tissue culture rabies vaccine (Verorab) in pre-exposure prophylaxis in humans].
abstract: BACKGROUND
  A WHO experts committee recommended the substitution of antirabic vaccines produced in nervous tissue, by vaccines produced in tissue cultures.
  
  
  OBJECTIVE
  To compare the immunogenic capacity of antirabic vaccines CRL (produced in nervous tissue) and Verorab (produced in tissue culture), used for pre-exposure prophylaxis in humans.
  
  
  METHODS
  Fifty four volunteers were immunized for this study. The first group, vaccinated with CLR was treated with a scheme of 4 subcutaneous peri umbilical doses in days 0, 3, 7 and 28. The second group, vaccinated with Verorab vaccine was treated with a scheme of 3 intramuscular doses in deltoid zone at days 0, 7 and 28. Blood samples were obtained at days 0, 7, 42 and 365 to measure neutralizing antibodies using the Inhibition of Fluorescent Focus Technique (RFFIT).
  
  
  RESULTS
  At day seven, a primary non protective immunologic response was observed in both groups, with titers significantly higher in the group vaccinated with Verorab. At day 42, no differences were observed. At day 365, all subjects vaccinated with Verorab and 50% of individuals vaccinated with CRL had protective antibody titers (p <0.05).
  
  
  CONCLUSIONS
  The vaccine produced in cell breeds (Verorab) produces a better an faster immunologic response compared to the CRL vaccine.
authors.affiliation: Médico Veterinario, Instituto de Salud Pública de Chile, Santiago. mfavi@ispch.cl
authors.forename: Myriam
authors.initials: M
authors.lastname: Favi
authors.forename: Verónica
authors.initials: V
authors.lastname: Yung
authors.forename: Orietta
authors.initials: O
authors.lastname: Roos
authors.forename: Luis
authors.initials: L
authors.lastname: Rodríguez
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Trujillo
authors.forename: Attia
authors.initials: A
authors.lastname: Acevedo
mesh_terms: D000328:Adult; D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000302:isolation & purification; D001921:Brain / Q000276:immunology / Q000821:virology; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009417:Nerve Tissue; D011819:Rabies Vaccines / Q000276:immunology*; D046509:Tissue Culture Techniques; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D011819:Rabies Vaccines
vernacular_title: Evaluación de la capacidad inmunogénica de la vacuna antirrábica tipo Fuenzalida- Palacios (CRL) y de la vacuna antirrábica de cultivo celular (Verorab) en personas con tratamiento preexposición.