Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles.
For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses.
Arakaki
Atsushi
A
Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3-4-1, Shinjuku-ku, Tokyo 169-8555, Japan.
Hideshima
Sho
S
Nakagawa
Takahito
T
Niwa
Daisuke
D
Tanaka
Tsuyoshi
T
Matsunaga
Tadashi
T
Osaka
Tetsuya
T
D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001710:Biotin / Q000032:analysis / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D008280:Magnetics; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods*; D019809:Streptavidin / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure
D020099:Coated Materials, Biocompatible; D001710:Biotin; D019809:Streptavidin