title: Daio-Orengedokudo works as a cell-proliferating compound in endothelial cells.
abstract: Daio-Orengedokuto is a combination drug that has inhibitory effects on HMG-CoA reductase and pancreatic lipase. Here we investigated whether Daio-Orengedokuto has effects on vascular endothelial cells. To determine its effects on blood vessels, we examined roles of Daio-Orengedokuto in cell migration, cell apoptosis and cell cycle progression over bovine aortic endothelial cells (BAECs). Interestingly, Daio-Orengedokuto was shown to work as an anti-apoptotic agent, a cell cycle progressive agent and a cell-migration inducing agent in BAECs, whereas it was known to act as a tumor suppressor in cancer cells (unpublished data). The inducing effect of Daio-Orengedokuto on cell-cycle progression and cell migration in endothelium suggests that Daio-Orengedokuto may be referred to as a drug, inducing angiogenesis, healing wounds, and (or) remodeling vascular tissue. Then we further investigated which signaling molecules were activated by Daio-Orengedokuto and found that extracellular signal-regulated kinase (ERK) phosphorylation and IkappaB degradation were stimulated by the Daio-Orengedokuto treatment in BAECs. More interestingly, pretreatment with PD compound, an ERK inhibitor, blocked the anti-apoptosis induced by Daio-Orengedokuto. In conclusion, Daio-Orengedokuto plays a role in endothelial cell proliferation via activation of MAP kinase.
authors.affiliation: College of Oriental Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Cho
authors.forename: Woo-Sang
authors.initials: WS
authors.lastname: Jung
authors.forename: Sung-Uk
authors.initials: SU
authors.lastname: Park
authors.forename: Sang-Kwan
authors.initials: SK
authors.lastname: Moon
authors.forename: Chang-Nam
authors.initials: CN
authors.lastname: Ko
authors.forename: Seojin
authors.initials: S
authors.lastname: Ku
authors.forename: Sung-Gil
authors.initials: SG
authors.lastname: Chi
authors.forename: Heonyong
authors.initials: H
authors.lastname: Park
mesh_terms: D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology
chemical_list: D004365:Drugs, Chinese Herbal; C061676:oren gedoku to