Gene expression profile in liver of hB1F transgenic mice.
OBJECTIVE
To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.


METHODS
Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR.


RESULTS
The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes.


CONCLUSIONS
The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.
Wang
Shui-Liang
SL
Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
Yang
Hua
H
Xie
You-Hua
YH
Wang
Yuan
Y
Li
Jian-Zhong
JZ
Wang
Long
L
Wang
Zhu-Gang
ZG
Fu
Ji-Liang
JL
D000818:Animals; D004268:DNA-Binding Proteins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D019076:Transgenes
D004268:DNA-Binding Proteins; C082631:NR5A2 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D015534:Trans-Activators; D014157:Transcription Factors