Evaluation of a LAM ELISA for diagnosis of paratuberculosis in sheep and goats.
A milk and serum ELISA containing lipoarabinomanan (LAM) antigen was evaluated in sheep and goats versus agar gel immunodiffusion (AGID) test and polymerase chain reaction (PCR) using milk and lymph nodes. Milk and serum samples were obtained from six, two, and four flocks with unknown, negative and positive status of infection, respectively. By comparison of serum ELISA activity and PCR results, the positive and negative predictive values were calculated. Receiver operation characteristic (ROC) analysis was used for calculating the specificity and sensitivity at different cut-offs.
Munjal
S K
SK
Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243122, UP, India.
Boehmer
J
J
Beyerbach
M
M
Strutzberg-Minder
K
K
Homuth
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D006041:Goats; D005779:Immunodiffusion / Q000662:veterinary; D008070:Lipopolysaccharides / Q000276:immunology*; D008892:Milk / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011237:Predictive Value of Tests; D015995:Prevalence; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C050016:lipoarabinomannan