title: Analysis of HLA-A24-restricted CMVpp65 peptide-specific CTL with HLA-A*2402-CMVpp65 tetramer.
abstract: Developing precise and efficient methods of monitoring immune responses against cytomegalovirus (CMV) infection in immunocompromised transplantation patients is important. With the aim of optimizing the monitoring strategy, an HLA-A24-CMVpp65 tetramer-based analysis of CMVpp65 peptide-specific CTL lines was performed. Previously, the HLA-A24-restricted CTL epitope of CMVpp65 matrix protein was identified (QYDPVAALF aa 341-349). In the present study, CMVpp65 (aa 341-349) peptide-specific CTL lines were obtained from PBLs of 12 HLA-A24+ healthy donors by two stimulations with peptide-pulsed dendritic cells (DC). Among 12 CTL lines, 9 showed HLA-A*2402-CMVpp65 tetramer staining, which was found to be strongly co-related to the amount of IFN-gamma produced by CMVpp65 peptide-restimulated CTL lines (r=0.943, P<0.001). These results suggested that HLA-A*2402-CMVpp65 tetramer staining was an efficient way to monitor immune responses against CMV infection in HLA-A24+ immunocompromised hosts.
authors.affiliation: National Cancer Center Research Institute, Growth Factor Division, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104, Japan. y.akiyama@scchr.jp
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kuzushima
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tsurumi
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000737:chemistry / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D015234:HLA-A Antigens / Q000276:immunology*; D059871:HLA-A24 Antigen; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology*; D020836:Protein Structure, Quaternary; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D015234:HLA-A Antigens; C438506:HLA-A*24:02 antigen; D059871:HLA-A24 Antigen; D007074:Immunoglobulin G; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D007371:Interferon-gamma