NMR structural model of the interaction of herbicides with the photosynthetic reaction center from Rhodobacter sphaeroides.
The interaction of the herbicides acifluorfen and paraquat with the photosynthetic reaction center from Rhodobacter sphaeroides has been studied by NMR relaxation measurements. Interaction in aqueous solution has been demonstrated by evaluating motional features of the bound form through cross-relaxation terms of protons at fixed distances on the herbicides. Contributions to longitudinal nonselective relaxation rates different from the proton-proton dipolar relaxation were inferred, most probably due to paramagnetic effects originating from the high-spin nonheme Fe(II) ion in the reaction center. Paramagnetic contributions to proton relaxation rates were converted into distance constraints in order to build a model for the interaction. The models place paraquat in the QB site, where most herbicides interact, in agreement with docking calculations, whereas acifluorfen was placed between the metal and the QB site, as also demonstrated by the induced paramagnetic shifts. Acifluorfen could therefore act to break the electron-transfer pathway between the QA and QB sites.
D'Amelio
Nicola
N
Department of Chemistry and the NMR Center, University of Siena, Via A. Moro, 53100 Siena, Italy.
Gaggelli
Elena
E
Mlynarz
Piotr
P
Molteni
Elena
E
Valensin
Gianni
G
Lubitz
Wolfgang
W
D018360:Crystallography, X-Ray; D006540:Herbicides / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009579:Nitrobenzoates / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects*; D011487:Protein Conformation; D012242:Rhodobacter sphaeroides / Q000187:drug effects* / Q000378:metabolism*
D006540:Herbicides; D009579:Nitrobenzoates; C018425:acifluorfen; D010269:Paraquat