title: Biochemical effects of vepacide (from Azadirachta indica) on Wistar rats during subchronic exposure.
abstract: We investigated the effect of Vepacide (from Azadirachta indica), a neem-based pesticide, on acid (AcP) and alkaline (AkP) phosphatase in different tissues of male and female albino Wistar rats. Subchronic doses of Vepacide in coconut oil (80, 160, and 320 mg/kg; maximum volume of 0.2 mL) were administered orally for 45 or 90 days. The administration of Vepacide resulted in a significant increase in AcP and AkP in serum, kidney, lung, and liver tissue (AkP only in liver), whereas a significant decrease of AcP in liver was observed in male and female rats after 45 and 90 days of treatment with moderate and high doses. The alterations in serum, liver, kidney, and lung tissues of both male and female rats caused by this compound were statistically significant, and the changes were also dose and time dependent. The alterations in male rats were not statistically significant when compared with female rats, indicating that there were no sexual differences. The withdrawal study (28 days post-treatment) revealed significant recovery, indicating reversal of the toxic symptoms once the toxicant was removed. There was a high degree of positive correlation between results for serum as compared to those for kidney, lung, and liver (AkP only for liver). However, there was a high negative correlation between AcP results for serum as compared with those for liver. The alterations in these enzymes indicated that lung tissue was the most susceptible, followed by liver and kidney. AcP and AkP are marker enzymes, and their increase in serum, with parallel increases in different tissues, might be due to the increased permeability of plasma membranes. The decrease in liver AcP may be due to the necrosis of cellular tissues. The changes observed in these enzyme activities could be useful as biomarkers of exposure to Vepacide.
authors.affiliation: Biochemical Toxicology, Biology Division, Indian Institute of Chemical Technology, Hyderabad 500 007, India. rahman@iict.ap.nic.in
authors.forename: M F
authors.initials: MF
authors.lastname: Rahman
authors.forename: M K J
authors.initials: MK
authors.lastname: Siddiqui
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D014018:Tissue Distribution; D014315:Triterpenes / Q000633:toxicity*
chemical_list: D014315:Triterpenes; C114225:Vepacide; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase