AT4 receptor binding in the developing rabbit.
The binding of the AT(4)-specific analog, divalinal-AngIV (Dival), was studied in rabbit fetuses of various gestational ages. Saturation isotherm and competition data from selected tissues indicate that fetal Dival binding sites are saturable and specific for AT(4) ligands. Autoradiographs revealed that binding was present in all the specimens examined. The peripheral nerves, kidneys, and heart were particularly heavily labeled. Labeling of some tissues, such as forming bones, was not constant as gestational age increased. Other tissues, including multilocular fat, sinus hairs, and enamel organs of nascent teeth, exhibited substantial binding as these tissues developed.
Burns
Gilbert A
GA
Department of Veterinary Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Room 205 Wegner Hall, Washington State University, Pullman, WA 99164, USA. gil_burns@wsu.edu
Ann-Marie
Thiessen
T
Jodie
Hanesworth
H
Harding
Joseph W
JW
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006321:Heart / Q000196:embryology / Q000502:physiology*; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D010525:Peripheral Nerves / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D011485:Protein Binding; D011817:Rabbits / Q000254:growth & development*; D011945:Receptors, Angiotensin / Q000378:metabolism*; D014018:Tissue Distribution
C090830:AT4 receptor; D011945:Receptors, Angiotensin; D000804:Angiotensin II