title: The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants.
abstract: Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli.
authors.affiliation: Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Flores
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bidnenko
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Michel
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology; D004265:DNA Helicases / Q000378:metabolism*; D004258:DNA Polymerase III / Q000378:metabolism; D004260:DNA Repair / Q000502:physiology*; D004261:DNA Replication / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000378:metabolism; D043211:Exodeoxyribonuclease V / Q000378:metabolism; D009154:Mutation
chemical_list: D029968:Escherichia coli Proteins; C074904:DNA polymerase III, alpha subunit; C101139:beta subunit, DNA polymerase III; D004258:DNA Polymerase III; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli; D000251:Adenosine Triphosphatases; C506746:UvrD protein, E coli; D004265:DNA Helicases