title: A method for the rapid depletion of albumin and immunoglobulin from human plasma.
abstract: In studies of the plasma proteome, the high abundance of proteins such as albumin and immunoglobulin impedes the investigation of lower abundance proteins that may be more suitable as biomarkers of disease. We report the specific removal of 98% of albumin and 80% of immunoglobulin heavy chain from human plasma by affinity chromatography, and the subsequent improvement in the number of spots detected and their resolution following two-dimensional gel electrophoresis.
authors.affiliation: Department of Pharmacology, University of Liverpool, UK.
authors.forename: Carrie
authors.initials: C
authors.lastname: Greenough
authors.forename: Rosalind E
authors.initials: RE
authors.lastname: Jenkins
authors.forename: Neil R
authors.initials: NR
authors.lastname: Kitteringham
authors.forename: Munir
authors.initials: M
authors.lastname: Pirmohamed
authors.forename: B Kevin
authors.initials: BK
authors.lastname: Park
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Pennington
mesh_terms: D000418:Albumins / Q000032:analysis*; D014408:Biomarkers, Tumor; D001798:Blood Proteins / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins / Q000097:blood*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000418:Albumins; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D020543:Proteome