title: Analysis of salivary peptides using HPLC-electrospray mass spectrometry.
abstract: Salivary peptides are involved in a wide range of functions constituting the first line of defence of oral cavity and precursors of dental pellicle formation. The presence of mucins in saliva makes difficult the analysis of the proteic content. This is due mainly to aggregation phenomenon between mucins and other high molecular weight glycoproteins and salivary proteins. Considering the importance of salivary peptides in biological functions, we have evaluated the influence of four different extraction methodologies on the separation and identification of these proteins by HPLC-MS. Based on their molecular weight, we identified a total of 22 peptides when extraction was performed using a solution of guanidine (6 m), compared with 14 peptides identified when saliva is acidified with TFA, which is an often used procedure. Our results also show the presence of mucin bind peptides, which include statherin, PRP1, PRP3, Histatin 1 and Histatin 5.
authors.affiliation: Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.forename: M João C
authors.initials: MJ
authors.lastname: Lobo
authors.forename: José A R
authors.initials: JA
authors.lastname: Duarte
authors.forename: A J
authors.initials: AJ
authors.lastname: Ferrer-Correia
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Domingues
authors.forename: Francisco M L
authors.initials: FM
authors.lastname: Amado
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019791:Guanidine; D006801:Humans; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D055232:Proline-Rich Protein Domains; D011506:Proteins / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid; D000099053:Histidine-Rich Glycoprotein
chemical_list: D009077:Mucins; D010455:Peptides; D011506:Proteins; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D012996:Solutions; D000099053:Histidine-Rich Glycoprotein; D014269:Trifluoroacetic Acid; D019791:Guanidine