title: 3-d reconstruction of 2-D crystals in real space.
abstract: A new algorithm for three-dimensional reconstruction of two-dimensional crystals from projections is presented, and its applicability to biological macromolecules imaged using transmission electron microscopy (TEM) is investigated. Its main departures from the traditional approach is that it works in real space, rather than in Fourier space, and it is iterative. This has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the TEM image formation process. Phantom experiments indicate the superiority of the new approach even without the introduction of constraints in addition to the projection data.
authors.affiliation: Escuela Politécnica Superior, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Marabini
authors.forename: Carlos O S
authors.initials: CO
authors.lastname: Sorzano
authors.forename: Samuel
authors.initials: S
authors.lastname: Matej
authors.forename: José J
authors.initials: JJ
authors.lastname: Fernández
authors.forename: José M
authors.initials: JM
authors.lastname: Carazo
authors.forename: Gabor T
authors.initials: GT
authors.lastname: Herman
mesh_terms: D000465:Algorithms; D017103:Bacillus Phages / Q000737:chemistry; D036022:Capsid Proteins / Q000737:chemistry / Q000648:ultrastructure; D003196:Computer Graphics; D003461:Crystallography / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D046911:Macromolecular Substances; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D008968:Molecular Conformation; D010363:Pattern Recognition, Automated; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
chemical_list: D036022:Capsid Proteins; D046911:Macromolecular Substances