Jannaschia cystaugens sp. nov., an Alexandrium (Dinophyceae) cyst formation-promoting bacterium from Hiroshima Bay, Japan.
Heterotrophic bacteria isolated from water samples taken from Hiroshima Bay, Japan, and referred to as Alexandrium (Dinophyceae) cyst formation-promoting bacteria, were assigned to the Roseobacter-Sulfitobacter-Silicibacter group within the alpha-Proteobacteria on the basis of nearly complete 16S rRNA gene sequences. Phylogenetic analyses showed that two strains, CFPB-A9T and CFPB-A5, are closely related to each other and that their closest relative was Jannaschia helgolandensis (95.9 % sequence similarity). These strains were Gram-negative, motile, obligately aerobic rods that required sodium ions and 2-7 % sea salts for growth and did not produce bacteriochlorophyll a. Their optimal growth temperature was 25-30 degrees C. The strains had Q-10 as the dominant respiratory quinone. Primary cellular fatty acid in both strains was 18 : 1omega7c. The DNA G + C contents of strains CFPB-A9T and CFPB-A5 were 59.1 and 59.2 mol%, respectively. Based on physiological, biological, chemotaxonomic and phylogenetic data, the strains are considered to represent a novel species, Jannaschia cystaugens sp. nov., with type strain CFPB-A9T (= LMG 22015T = NBRC 100362T).
Adachi
Masao
M
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan.
Kanno
Tsukasa
T
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan.
Okamoto
Ryo
R
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan.
Shinozaki
Azusa
A
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan.
Fujikawa-Adachi
Kiyomi
K
Department of Molecular and Cellular Biology, Kochi Medical School, Kochi 783-8505, Japan.
Nishijima
Toshitaka
T
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan.
D025541:Bacteriochlorophyll A / Q000032:analysis; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007564:Japan; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D012492:Salts; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology
D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts