title: Increased osteoblast functions on theta + delta nanofiber alumina.
abstract: Nanophase materials, or materials with grain sizes less than 100 nm in at least one direction, are promising materials for various implant applications since our tissues are composed of nanometer components (i.e., proteins and/or inorganics). Specifically, bone is comprised of nanostructured hydroxyapatite and collagen fibers which continuously provide an extracellular matrix surface to bone-forming cells (osteoblasts) with a high degree of nanometer roughness. Despite this fact, materials currently utilized for orthopedic implants, whether metallic or ceramic, have constituent grain sizes in the non-biologically inspired micron regime. For this reason, the objective of the present in vitro study was to determine osteoblast functions on one classification of nanomaterials for orthopedic applications: nanofiber alumina. Various crystalline forms of nanofiber alumina were tested in this study. To obtained different crystalline structured nanofiber alumina, boehmite nanofiber alumina was sintered at either 400 degrees C, 600 degrees C, 800 degrees C, 1000 degrees C, or 1200 degrees C for 2 h in air. X-ray diffraction results provided evidence that boehmite nanofiber alumina remained boehmite when sintered at 400 degrees C but changed crystalline phases to gamma, gamma + delta, theta + delta, and alpha when sintered at 600 degrees C, 800 degrees C, 1000 degrees C, and 1200 degrees C, respectively. Moreover, compared to any other alumina formulation tested in this study, osteoblast functions (as measured by alkaline phosphatase activity and calcium deposition) were the greatest on theta + delta crystalline phase nanofiber alumina after 14 days of culture. Boehmite had the next greatest amount of calcium deposition by osteoblasts followed by gamma + delta. Gamma crystalline phase then followed and was greater than alpha crystalline phase nanofiber alumina which promoted osteoblast functions the least of all the compacts with the exception of borosilicate glass (reference substrate). For this reason, this study suggests that theta+delta nanofiber alumina should be further investigated in orthopedic applications.
authors.affiliation: Department of Biomedical Engineering, Purdue University, Potter Building, West Lafayette, IN 47907-1296, USA. twebster@ecn.purdue.edu
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.forename: Elaine L
authors.initials: EL
authors.lastname: Hellenmeyer
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Price
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D016326:Extracellular Matrix Proteins; D000537:Aluminum Oxide; D002118:Calcium