Regeneration of pea (Pisum sativum L.) by a cyclic organogenic system.
In a five-step procedure, plants were regenerated from meristematic tissue initiated from nodal tissue in four pea cultivars ('Espace', 'Classic', 'Solara', and 'Puget'). In step 1, stem tissue with one node (1-cm size) was subcultured on medium containing thidiazuron. As a result multiple shoots were produced, appearing normal or swollen at their bases. The multiple shoots were subcultured in the same medium, resulting in the formation of a green hyperhydric tissue in the swollen bases of the multiple shoots, which is fully covered with small buds [bud-containing tissue (BCT)]. In step 2, BCT fragments were isolated and subcultured in the same medium and, as a result, they were able to reproduce themselves in a cyclic fashion. In step 3, subculture of BCT on medium supplemented with a combination of gibberelic acid, 6-benzyladenine and alpha-naphthalene acetic acid (NAA), resulted in the formation of shoots, which were rooted in step 4 on medium supplemented with 0.5 mg/l NAA, indole-3-acetic acid (IAA) or indole-3-butyric acid. In step 5, in vitro plants were transferred to the greenhouse for acclimatisation and further development. The four varieties tested were all able to produce meristematic tissue, suggesting that its production is genotype independent.
Tzitzikas
Emmanouil N
EN
Laboratory of Plant Breeding, The Graduate School Experimental Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.
Bergervoet
Marjan
M
Raemakers
Krit
K
Vincken
Jean-Paul
JP
van Lammeren
Andre
A
Visser
Richard G F
RG
D000064:Acclimatization / Q000502:physiology; D000383:Agriculture / Q000379:methods; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D018519:Meristem / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018532:Pisum sativum / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media; D010937:Plant Growth Regulators