Osteoblast attachment to a textured surface in the absence of exogenous adhesion proteins.
The present study investigated whether osteoblasts could attach to a culture substratum through a surface texture-dependent mechanism. Four test groups were used: (A) untextured, and three texture groups with maximum feature sizes of (B) <0.5 microm, (C) 2 microm, and (D) 4 microm, respectively. All surfaces were coated with the nonadhesive protein bovine serum albumin (BSA). Osteoblasts were allowed to adhere in serum-free medium for either 1 or 4 h, at which time nonadherent cells were removed. At 4 h, untextured surface A exhibited no cell attachment, while textured surfaces B, C, and D exhibited 9%, 32%, and 16% cell adhesion, respectively. At 16 h of incubation, adherent osteoblasts on textured surface C exhibited focal adhesion contacts and microfilament stress-fiber bundles. These results indicate that microtextured surfaces in the absence of exogenous adhesive proteins can facilitate osteoblast adhesion.
Mata
Alvaro
A
Department of Biomedical Engineering (ND 20), Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195 USA. mataa@bme.ri.ccf.org
Su
Xiaowei
X
Fleischman
Aaron J
AJ
Roy
Shuvo
S
Banks
Bruce A
BA
Miller
Sharon K
SK
Midura
Ronald J
RJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D017402:Chlorofluorocarbons / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D008422:Materials Testing; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D019038:Platelet Glycoprotein GPIb-IX Complex; D019736:Prostheses and Implants; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials; D017402:Chlorofluorocarbons; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D012710:Serum Albumin, Bovine; C016304:chlorotrifluoroethylene