title: In vitro study of human vascular endothelial cell function on materials with various surface roughness.
abstract: In recent years, creating a biodegradable polymer scaffold with an endothelialized surface has become an attractive concept for replacement of small-diameter blood vessels. Toward this end, a better understanding of the interaction between endothelial cells and biodegradable polymer substrates is particularly important. Surface roughness of biomaterials is one of the important parameters that affect cell behavior. In this study, human vascular endothelial cells were cultured on electrospun and solvent-cast poly(L-lactic acid) substrates with different surface roughness. Cell responses were evaluated via both qualitative examinations of cell morphology changes as well as quantitative assessment of cell adhesion and proliferation rate on the different substrates. The results proved that endothelial cell function was enhanced on the smooth solvent-cast surface rather than on the rough electrospun surface of poly(L-lactic acid). Together with our previous findings that electrospun substrates favor vascular smooth muscle cell behavior, it is possible to design a unique three-dimensional scaffold for application of tissue-engineered small-diameter vessel replacement by combining the fabrication technique of solvent casting and electrospinning.
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576. engxc@nus.edu.sg
authors.forename: Chengyu
authors.initials: C
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)