Effect of supplementation with methionine and different fat sources on the glutathione redox system of growing chickens.
The effect of supplementary methionine and fats of different saturation levels on the glutathione redox system of growing broiler cockerels was studied. The diet of three groups of chicks was supplemented with corn germ oil, beef tallow and fish oil at the levels of 30 g/kg and 50 g/kg of feed, respectively. The diet of further three groups was supplemented with methionine (5 g/kg of feed) in addition to the different fat sources. Control chicks were fed with a compound feed without methionine and fat supplementation. Reduced glutathione (GSH) and glutathione disulphide (GSSG) content as well as glutathione peroxidase activity in the liver were determined and GSH/GSSG ratio was calculated at day old and then at one and three weeks of age. Our results indicate that supplementary methionine stimulates both the synthesis of the glutathione redox system and glutathione peroxidase activity in growing chickens in the first period of postnatal life, when the risk of lipid peroxidation is high due to feeding unsaturated fats in the diet.
Németh
Katalin
K
Department of Animal Physiology and Animal Nutrition, Georgikon Faculty of Agriculture, Veszprém University, H-8360 Keszthely, Deák F u 16, Hungary.
Mézes
M
M
Gaál
T
T
Bartos
A
A
Balogh
K
K
Husvéth
F
F
D000821:Animal Feed; D000818:Animals; D016022:Case-Control Studies; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D003314:Corn Oil / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage* / Q000145:classification; D019587:Dietary Supplements; D005223:Fats / Q000008:administration & dosage; D005395:Fish Oils / Q000008:administration & dosage; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008054:Lipid Peroxides / Q000032:analysis; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage*; D010084:Oxidation-Reduction
D004041:Dietary Fats; D005223:Fats; D005395:Fish Oils; D008054:Lipid Peroxides; D003314:Corn Oil; C013698:tallow; D008715:Methionine; D005979:Glutathione Peroxidase; D005978:Glutathione; D019803:Glutathione Disulfide