Distinct temporal genetic signatures of neurogenic and gliogenic cues in cortical stem cell cultures.
Cortical progenitor cells from rat embryos give rise to neurons or glia following exposure to platelet derived growth factor (PDGF) or ciliary neurotrophic factor (CNTF), respectively. Both growth factors impart their developmental cues quickly through a transcription-dependent mechanism. Do the alternate developmental responses to PDGF and CNTF reflect induction of qualitatively distinct genes? Alternatively, do the same genes respond to each growth factor, but with quantitatively distinct kinetics? Using differential library screening and custom cDNA microarrays we show that a common set of genes responds to either growth factor. However, quantitative differences in the onset and duration of gene induction equate to the expression of factor-specific gene signatures. Multitissue cluster analysis also reveals tissue-specific gene signatures that may play important roles in the developing brain.
Sauvageot
Claire
C
Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Dahia
Patricia L
PL
Lipan
Ovidiu
O
Park
John K
JK
Chang
Mi-Sook
MS
Alberta
John A
JA
Stiles
Charles D
CD
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000196:embryology* / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor / Q000378:metabolism / Q000494:pharmacology; D016000:Cluster Analysis; D003463:Cues; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics*; D015723:Gene Library; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D015533:Transcriptional Activation
D020934:Ciliary Neurotrophic Factor; D010982:Platelet-Derived Growth Factor