Inhibition of lysosomal cysteine proteases by chrysotherapeutic compounds: a possible mechanism for the antiarthritic activity of Au(I).
Although Au(I) complexes have been used to treat rheumatoid arthritis for over 75 years, their mechanism of action is still poorly understood. A family of enzymes responsible for joint destruction in rheumatoid arthritis, the cathepsins, has been discussed as a possible biological target of Au(I). In this study, inhibition of the cathepsins by known Au(I) drugs and related compounds was investigated. The compounds tested inhibited cathepsin activity with IC50 values as low as 600 nM. More typical IC50 values were in the 50-200 microM range. Although the gold complexes are not extremely potent cathepsin inhibitors, it is likely that this inhibition is biologically relevant given the high concentrations of Au(I) in the serum and joints of patients undergoing chrysotherapy. While it is likely that there are multiple targets of Au(I) in vivo, inhibition of the cathepsins would provide protection against the joint destruction that is a hallmark of rheumatoid arthritis and is one possible mechanism for Au(I) antiarthritic activity.
Chircorian
Aida
A
Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
Barrios
Amy M
AM
D000818:Animals; D018501:Antirheumatic Agents / Q000737:chemistry*; D001310:Auranofin / Q000737:chemistry*; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000737:chemistry; D056668:Cathepsin L; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000737:chemistry; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry*; D017612:Gold Compounds / Q000737:chemistry*; D006052:Gold Sodium Thiomalate / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D016805:Paramecium tetraurelia
D018501:Antirheumatic Agents; D002712:Chlorides; D015853:Cysteine Proteinase Inhibitors; D017612:Gold Compounds; D006052:Gold Sodium Thiomalate; D001310:Auranofin; C024568:gold tetrachloride, acid; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L