Characterization of the P5 subfamily of P-type transport ATPases in mice.
In mammals, the most poorly understood P-type ATPases are those of the P(5) subfamily. To begin characterization of the mammalian P(5)-ATPases, BLAST searches of DNA sequence databases were performed. Five genes were identified in the mouse, human, dog, and rat genomes, and the coding sequences of the mouse genes, termed Atp13a1-Atp13a5, were determined. The intron/exon organization of Atp13a1 differs entirely from those of Atp13a2-5, which are closely related. Amino acid sequence comparisons between the five mouse and two yeast P(5)-ATPases suggest that Atp13a1 is orthologous to the yeast Cod1 gene and that Atp13a2-5 are orthologous to yeast Yor291w. Northern blot analysis showed that Atp13a1, Atp13a2, and Atp13a3 mRNAs were expressed in all mouse tissues, whereas Atp13a4 and Atp13a5 mRNAs were restricted to brain and stomach. While the substrate specificity of these transporters is unknown, their importance is underscored by the presence of homologs in fish, insects, worms, and other eukaryotes.
Schultheis
Patrick J
PJ
Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY 41099, USA. schultheisp@exchange.nku.edu
Hagen
Tamara T
TT
O'Toole
Kate K
KK
Tachibana
Akiko
A
Burke
Charles R
CR
McGill
Diana L
DL
Okunade
Gbolahan W
GW
Shull
Gary E
GE
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000252:Calcium-Transporting ATPases; D002874:Chromosome Mapping / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009928:Organ Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases