[Interaction between osteoblasts and fibroblasts derived from same individual].
OBJECTIVE
In order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the interaction between osteoblasts and fibroblasts.


METHODS
The cell co-culture model was established by cell culture inserts. The expression difference of protein related to mineralization (ALP, Type I collagen) between these two kinds of cells cultured individually and co-cultured were evaluated.


RESULTS
Co-culture changed the ALPase activity of the two kinds of cells greatly .The ALP expression of osteoblasts decreased, but the ALP expression of periodontal ligament fibroblasts increased. The Col-I expression of these two kinds of cells did not change significantly when they were co-cultured.


CONCLUSIONS
Periodontal ligament fibroblast could restrain the osteogenesis function of osteoblast, while osteoblast could induce the differentiation of fibroblast when both of them existed in periodontal tissue.
Gong
Ping
P
Key Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Tan
Zhen
Z
Zhao
Qing
Q
D000469:Alkaline Phosphatase / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology
D024042:Collagen Type I; D000469:Alkaline Phosphatase