Protective effect of bovine milk whey protein concentrate on the ulcerative lesions caused by subcutaneous administration of indomethacin.
The protective effect of a whey protein concentrate (WPC) was studied in three models of stomach ulcerative lesions induction: subcutaneous injection of indomethacin, and stress induced by either intraperitoneal injection of reserpine, or immobilization and holding in the cold (4 degrees C, 2 hours). Adult Wistar rats (300-400 g) were used for acute (single-dose), repetitive, or subchronic (10 days) administration of WPC prior to treatment with the ulcerogenic factors. The best protection was achieved in the indomethacin model for repetitive and subchronic experiments, reaching 50.1% and 44%, respectively, inhibition of the ulcerative lesions, which was significant at 1% probability (P <.01). For the immobilization and cold model, maximum inhibition by WPC was 22.1%, and that for the reserpine model was 23.8%. In both models the inhibition was not significant (P >.05) compared with saline (negative control).
Rosaneli
C F
CF
Departamento de Alimentos e Nutrição, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Campinas, São Paulo, Brasil.
Bighetti
A E
AE
Antônio
M A
MA
Carvalho
J E
JE
Sgarbieri
V C
VC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D000897:Anti-Ulcer Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002417:Cattle; D002927:Cimetidine / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D007103:Immobilization; D007213:Indomethacin / Q000633:toxicity; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012110:Reserpine / Q000494:pharmacology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D000067816:Whey Proteins
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D008894:Milk Proteins; D000067816:Whey Proteins; D002927:Cimetidine; D012110:Reserpine; D007213:Indomethacin