[Interferon-inducible genes lymphocyte antigen 6 complex E and tetratricopeptide repeats 1 are correlated with clinical features of patients with systemic lupus erythematosus].
OBJECTIVE
To investigate the expression levels of lymphocyte antigen 6 complex, locus E (LY6E) and interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) genes in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity.


METHODS
The clinical data of 144 SLE patients, 27 non-SLE patients with rheumatic diseases, and 59 normal controls were collected. The SLE patients were further divided into 2 subgroups: active SLE group (n = 87) and non-active SLE group (n = 57) according to SLEDAI scores. Specimens of peripheral blood were drawn; total RNA was extracted and transcribed into cDNA. Sybr green dye based real-time quantitative PCR method was used to compare the expression levels (indicated as-DeltaDeltaCT value) of LY6E and IFIT1 in patients with SLE and those in the controls.


RESULTS
(1) The-DeltaDeltaCT value of LY6E expression level of the SLE patients was 5.4760 +/- 1.9806, significantly higher than those of the non-SLE patients (3.4323 +/- 1.7456) and normal controls (4.5198 +/- 1.6359, both P = 0.001). (2) The-DeltaDeltaCT value of LY6E and IFIT1 mRNA expression of the active SLE patients were 6.1960 +/- 1.7729 and 6.4997 +/- 2.6297 respectively, significantly higher than those observed in the inactive SLE patients (4.3770 +/- 1.7764 and 4.1327 +/- 2.6044 respectively, both P = 0.000). The-DeltaDeltaCT values of LY6E and IFIT1 mRNA of the SLE patients were correlated with the SLEDAI scores, and with the numbers of matched criteria used in the diagnosis of SLE (P < 0.001).


CONCLUSIONS
As IFN-induced genes, elevated expression of LY6E mRNA is specific to diagnosis of SLE. The real time expression levels of LY6E and IFIT1 genes are associated with SLE disease activity. To inhibit the expression of LY6E and IFIT1 may become a novel therapeutic approach for SLE.
Tang
Jian-ping
JP
Shanghai Clinical Centre of Rheumatic Diseases and Institute of Rheumatology, Department of Rheumatology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Gu
Yue-ying
YY
Shen
Nan
N
Ye
Shuang
S
Qian
Jie
J
Hua
Jing
J
Chen
Xing-guo
XG
Chen
Shun-le
SL
Bao
Chun-de
CD
Wang
Yuan
Y
Zhang
Wei
W
D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002648:Child; D003001:Cloning, Molecular; D005260:Female; D058851:GPI-Linked Proteins; D020869:Gene Expression Profiling / Q000379:methods; D020022:Genetic Predisposition to Disease; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D016601:RNA-Binding Proteins; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D000954:Antigens, Surface; D002352:Carrier Proteins; D058851:GPI-Linked Proteins; C484290:IFIT1 protein, human; C439870:LY6E protein, human; D008565:Membrane Proteins; D009841:Oligonucleotides; D010455:Peptides; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D007372:Interferons