[Comparative characteristics of reproduction of the wheat striped mosaic virus in winter and spring Triticum aestivum L. in natural agrocoenosis and during clinostatting].
Microgravity (a transformed environment) was produced with the use of a multi-purpose clinostat. Object of the investigation was wheat striped mosaic virus (WSMV) affecting a great variety of wheat species in natural agrocoenosis, and super-dwarf cultivar Apogee in the transformed environment. Enzyme immunodetection (das-ELISA) as well as electron microscopy were employed for virus identification. Viral reproduction was found high (titre 1/2560) in winter and spring wheat species in agrocoenosis (natural transmissible background). Clinostatting in the horizontal and vertical planes (R = 400 mm and R = 250 mm) inhibited virus reproduction by day 21, 25 and 18 post inoculation, respectively. Clinostatting improved health of WSMV-infected plants and resulted in harvesting the first Apogee crop. Productivity of WSMV-infected plants after clinostatting was increased owing to a large seed content of the heads as compared with stationary and clinostatted healthy crops. This experience of inhibiting virus reproduction should be taken into consideration in virus-free seed production biotechnology development.
Mishchenko
L T
LT
D000818:Animals; D018504:Ecological Systems, Closed; D004797:Enzyme-Linked Immunosorbent Assay; D006824:Hybridization, Genetic / Q000235:genetics; D009029:Mosaic Viruses / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D012100:Reproduction, Asexual / Q000235:genetics*; D012621:Seasons; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins
Sravnitel'naia kharakteristika reproduktsii virusa polosatoĭ mozaiki pshenitsy v rasteniiakh Triticum aestivum L. ozimykh i iarovykh sortov v prirodnykh agrotsenozakh i v usloviiakh klinostatirovaniia.