Short communication: amino acid transporters in porcine mammary gland during lactation.
The objective of this study was to determine whether mRNA transcripts for the amino acid (AA) transporter proteins CAT-1, CAT-2B, B(0,+), and ASCT1 are present in porcine mammary tissue (MT). Transcript abundance on d 7 and 17 of lactation was determined by Northern blot analysis, and absolute quantification was performed by real-time polymerase chain reaction. Porcine MT expresses CAT-1, CAT-2B, B(0,+), and ASCT1 during lactation. Preliminary findings indicate that B(0,+) mRNA abundance tended to decrease on d 17 compared with that on d 7.
Laspiur
J Pérez
JP
Department of Animal Science, Michigan State University, East Lansing 48824, USA.
Burton
J L
JL
Weber
P S D
PS
Kirkwood
R N
RN
Trottier
N L
NL
D027182:Amino Acid Transport System y+ / Q000235:genetics; D026905:Amino Acid Transport Systems / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D027201:Cationic Amino Acid Transporter 1 / Q000235:genetics; D027202:Cationic Amino Acid Transporter 2 / Q000235:genetics; D005260:Female; D006801:Humans; D007774:Lactation; D008321:Mammary Glands, Animal / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D013552:Swine / Q000378:metabolism*; D013997:Time Factors
D027182:Amino Acid Transport System y+; D026905:Amino Acid Transport Systems; D027201:Cationic Amino Acid Transporter 1; D027202:Cationic Amino Acid Transporter 2; D012333:RNA, Messenger