Evolution of 8p loss in transformed human prostate epithelial cells.
Deletion or rearrangement of sequences that map to the short arm of chromosome 8 (8p) are frequently associated with human prostate tumorigenesis. These losses often involve the entire short arm of chromosome 8 or very large regions of distal or proximal 8p, and several putative tumor suppressor genes mapping to 8p have been described. However, the mechanism responsible for 8p loss during prostate tumorigenesis has not been elucidated. In this study, we report data obtained using array comparative genomic hybridization and spectral karyotyping, which demonstrate successive translocation and deletion events responsible for loss of one copy of 8p in transformed human prostate epithelial cells. Moreover, this loss was accompanied by a pronounced transcriptional downregulation of genes mapping to the remaining copy of 8p and enhanced expression of traits associated with neoplastic transformation. Taken together, these studies illustrate a potential mechanism and functional role for 8p loss in human prostate tumorigenesis.
Macoska
Jill A
JA
Department of Urology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0944, USA. joska@umich.edu
Paris
Pamela
P
Collins
Colin
C
Andaya
Armann
A
Beheshti
Ben
B
Chaib
Hassan
H
Kant
Rajiv
R
Begley
Lesa
L
MacDonald
James W
JW
Squire
Jeremy A
JA
D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002872:Chromosome Deletion; D002898:Chromosomes, Human, Pair 8; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007621:Karyotyping; D008297:Male; D010641:Phenotype; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic; D014178:Translocation, Genetic