[Xanthones from Tibetan medicine Halenia elliptica and their antioxidant activity].
OBJECTIVE
To investigate the xanthones from Tibetan medicine Halenia elliptica and their antioxidant activity.


METHODS
Column chromatography over normal phase silica gel, reversed phase silica gel, Sephadex LH-20, and recrystallization techniques were used to isolate and purify constituents from Halenia elliptica. Infrared spectrometry, mass spectrometry, and nuclear magnetic resonance spectrometry were used to identify the structure of compounds. The antioxidant activity was evaluated by measuring the content of malondialdehyde product in mice liver cell microsomal induced by ferrous-cysteine.


RESULTS
Eight xanthones (compound I-VIII) were isolated and identified from the ethyl acetate extract of Halenia elliptica, among which 1,7-dihydroxy-2,3,5-trimethoxyxanthone was a novel compound. Compound I, III at 10 microg/ml and 100 microg/ml could inhibit the production of malondialdehyde in mouse liver microsomes in vitro.


CONCLUSIONS
Eight xanthones were isolated and they have certain antioxidant activity.
Gao
Jie
J
Department of Chemistry of Natural Products, Institute of Materia Medica, CAMS and PUMC, Beijing 100050, China.
Wang
Su-juan
SJ
Fang
Fang
F
Si
Yi-kang
YK
Yang
Yong-chun
YC
Liu
Geng-tao
GT
Jian-gong
Shi
S
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029965:Gentianaceae / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D014966:Xanthenes / Q000302:isolation & purification / Q000494:pharmacology; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
C478004:1-hydroxy-2,3,5-trimethoxyxanthone; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D014966:Xanthenes; D044004:Xanthones