Extensive antigenic changes in an atypical isolate of very virulent infectious bursal disease virus and experimental clinical control of this virus with an antigenically classical live vaccine.
The 99323 Egyptian isolate of infectious bursal disease (IBD) virus (IBDV) was identified during an international survey of acute IBD cases. Its unique antigenicity was characterized by a markedly reduced binding of neutralizing monoclonal antibodies 3, 4, 5, 6, 8 and 9 in an antigen-capture enzyme-linked immunosorbent assay. Nucleotide sequencing of the genome region encoding the VP2 major immunogenic domain in 99323 revealed amino acid changes occurring at positions critical for antigenicity, but phylogenetic analysis demonstrated that 99323 was related to typical, very virulent IBDV (e.g. isolate 89163). Protection experimentally afforded by an antigenically classical live IBD vaccine was investigated in specific pathogen free chickens challenged with 99323 or 89163. Both viruses were similarly controlled, as evaluated by clinical signs, growth retardation, bursa-to-body weight ratios and histological lesions of the bursa after challenge. These results document that an active antibody response to a classical live antigen may clinically control infection by an antigenically atypical very virulent IBDV.
Eterradossi
Nicolas
N
Avian and Rabbit Virology, Immunology and Parasitology Unit (VIPAC), Ploufragan, France. n.eterradossi@ploufragan.afssa.fr
Gauthier
Céline
C
Reda
Ismail
I
Comte
Sylvain
S
Rivallan
Gaëlle
G
Toquin
Didier
D
de Boisséson
Claire
C
Lamandé
Josiane
J
Jestin
Véronique
V
Morin
Yannick
Y
Cazaban
Christophe
C
Borne
Pierre-Marie
PM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D011201:Poultry Diseases / Q000473:pathology / Q000517:prevention & control* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014611:Vaccination / Q000662:veterinary*; D015678:Viral Structural Proteins / Q000235:genetics
D000911:Antibodies, Monoclonal; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins