title: [Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes].
abstract: OBJECTIVE
  To observe the immunosuppression effect of a novel HLA-derived peptide RDP1258, and to explore its mechanisms.
  
  
  METHODS
  RDP1258 was synthesized chemically. The effect of the peptide on alloreactive proliferation of rat splenocytes was observed by (3)H-TdR incorporation method. The heme oxygenase-1(HO-1) activity was analyzed by the enzyme assay.
  
  
  RESULTS
  The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of rat splenocytes and HO-1 activity in a dose-dependent manner in-vitro.
  
  
  CONCLUSIONS
  HO-1 may participate in the inhibitory effect of RDP1258 on the proliferation of rat splenocytes induced by mitogen or alloantigen.
authors.affiliation: Department of Urology, Xingqiao Hospital, Third Military Medical University, Chongqing, 400037, China. yshh881@sohu.com
authors.forename: Shan-hong
authors.initials: SH
authors.lastname: Yi
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Ze-hou
authors.initials: ZH
authors.lastname: Wang
authors.forename: Gen-fu
authors.initials: GF
authors.lastname: Zhang
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Cui
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology*
chemical_list: D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; C122045:RDP 1258; D051547:Heme Oxygenase-1