title: Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices.
abstract: Application of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) for detection of aldehydes in biological samples such as blood, yoghurt, and milk, is reported. Sample preparation is easy, and the presented method is both sensitive and selective. It is based on the widely used dinitrophenylhydrazine derivatization, followed by extraction with n-hexane and a simple reversed-phase HPLC separation. Detection is performed by atmospheric pressure chemical ionization (APCI) in negative ion mode, with detection limits in the low picogram range. Using MS/MS, acetone and propionaldehyde can clearly be distinguished, facilitating propionaldehyde quantitation even in the presence of high acetone levels. Quantitation by direct MS/MS is also feasible, well suited for high-throughput applications, although not as accurate as using HPLC/MS/MS.
authors.affiliation: Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, H-1025 Pusztaszeri út 59-67, Budapest, Hungary.
authors.forename: Kornél
authors.initials: K
authors.lastname: Nagy
authors.forename: Ferenc
authors.initials: F
authors.lastname: Pollreisz
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Takáts
authors.forename: Károly
authors.initials: K
authors.lastname: Vékey
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D000818:Animals; D001274:Atmospheric Pressure; D001774:Blood Chemical Analysis / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D015014:Yogurt / Q000032:analysis*
chemical_list: D000447:Aldehydes; D006834:Hydrazines; C446799:dinitrophenylhydrazine