Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.
The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.
Henry-Stanley
Michelle J
MJ
Department of Laboratory Medicine and Pathology, Box 609 Mayo Building, 420 Delaware Street, S.E., University of Minnesota, Minneapolis 55455, USA.
Garni
Robb M
RM
Wells
Carol L
CL
D001557:Benzenesulfonates / Q000737:chemistry* / Q000378:metabolism; D018938:Caco-2 Cells; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D020895:Enterocytes / Q000382:microbiology*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D011806:Quinolinium Compounds / Q000737:chemistry* / Q000378:metabolism
D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C007061:C.I. Fluorescent Brightening Agent 28