Vitamin E in early stages of sea bass (Dicentrarchus labrax) development.
This study reports titration of vitamin E levels in the sea bass (Dicentrarchus labrax) using high-pressure liquid chromatography. The first part of the work is devoted to vitamin E detection in: (1) plasma of maturing females and males characterized by different body sizes; (2) seminal fluid and eggs; and (3) developing embryos of sea bass fed with vitamin E. In the second part of the study, variations of vitamin E levels during larval development are analyzed. The results show a direct correlation between plasma vitamin E content and body size for both adult male and female sea bass. High vitamin E levels were found in seminal fluid, in eggs before and after fertilization, and in embryos during development and at hatching, whereas vitamin E level was low in dead embryos and in embryos with limited survival. During larval development, the vitamin E content decreased slowly but steadily during the first four days of larval growth; subsequently, it progressively increased from day 9 to day 40. In teratogenic larvae, vitamin E content was significantly higher than in normal larvae. This study provides evidence on how vitamin E exerts an antioxidant defense in sea bass reproduction.
Guerriero
G
G
Department of Zoology, Federico II University of Naples, Via Mezzocannone, 8-80134 Napoli, Italy. giulia.guerriero@unina.it
Ferro
R
R
Russo
G L
GL
Ciarcia
G
G
D000818:Animals; D001492:Bass / Q000097:blood / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D049628:Body Size; D001835:Body Weight; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005306:Fertilization / Q000502:physiology; D007814:Larva / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D008297:Male; D010063:Ovum / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D014810:Vitamin E / Q000032:analysis / Q000097:blood / Q000378:metabolism*
D014810:Vitamin E