Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography-ultraviolet detection analysis.
Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards.
Mateo
Rafael
R
Instituto de Investigación en Recursos Cinegéticos, CSIC-UCLM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain. rafael.mateo@uclm.es
Castells
Gloria
G
Green
Andy J
AJ
Godoy
Carlo
C
Cristòfol
Carles
C
D000818:Animals; D001646:Bile / Q000737:chemistry*; D001664:Biliverdine / Q000032:analysis*; D001717:Birds / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004372:Ducks; D004784:Environmental Monitoring; D005243:Feces / Q000737:chemistry*; D005777:Geese; D007202:Indicators and Reagents; D007855:Lead Poisoning / Q000378:metabolism; D011166:Porphyrins / Q000032:analysis*; D020304:Raptors; D015203:Reproducibility of Results; D013048:Specimen Handling; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D011166:Porphyrins; D001664:Biliverdine