title: Argentine strain of equine herpesvirus 1 isolated from an aborted foetus shows low virulence in mouse respiratory and abortion models.
abstract: The equine herpesvirus 1 (EHV-1) was isolated in Argentina from an aborted equine foetus in 1979. This virus (SPv) has special restriction patterns (RP) in comparison with other Argentine isolates. In addition, SPv could be distinguished on the basis of its pathogenicity in baby mice inoculated intracerebrally. We studied the growth properties of the SPv in cell culture and its effects in a mouse respiratory and abortion model. We observed that SPv did not modify its capacity to grow in cell culture with respect to reference HH1 strain. Nevertheless, we found significant differences between the titres of the two strains at 8-14 h post-infection (PI). In this work we demonstrated that SPv showed low virulence in female at different stages of gestation, consistently, with results found in the mouse respiratory model. We considered that this low virulence of SPv could be related to its RP because the RP of HH1 strain are similar to those of the HVS25A strain and both showed effect on pregnant mice. More specific studies about genomic alterations to the SPv are necessary for identifying, more clearly, if the intra-strain variations have relation with the low virulence in the mouse respiratory and abortion model.
authors.affiliation: Department of Virology, Faculty of Veterinary Sciences, National University of La Plata, 60 and 118, P.O. Box 296, 1900 La Plata, Bs. As., Argentina. cmgalosi@fcv.unlp.edu.ar
authors.forename: C M
authors.initials: CM
authors.lastname: Galosi
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.forename: M V
authors.initials: MV
authors.lastname: Vila Roza
authors.forename: V
authors.initials: V
authors.lastname: Cid de la Paz
authors.forename: M A
authors.initials: MA
authors.lastname: Ayala
authors.forename: S G
authors.initials: SG
authors.lastname: Corva
authors.forename: M E
authors.initials: ME
authors.lastname: Etcheverrigaray
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001118:Argentina; D001835:Body Weight; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005313:Fetal Death / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D006734:Horse Diseases / Q000473:pathology / Q000821:virology*; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D013047:Specific Pathogen-Free Organisms; D014774:Virulence; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral