[The study of regulation of connective tissue growth factor gene promoter by transforming growth factor beta1 in pancreatic stellate cells].
OBJECTIVE
To investigate the molecular mechanism for transforming growth factor beta(1) (TGF-beta(1)) on regulation of connective tissue growth factor (CTGF) gene promoter in pancreatic stellate cells (PSCs).


METHODS
We tried to transfect the passaged 2 approximately 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-beta(1) or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system.


RESULTS
TGF-beta(1) could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-beta(1) could stimulate the expression of CTGF gene promoter at the high level in a short time.


CONCLUSIONS
TGF-beta(1) could enhance the activities of CTGF promoter in PSCs.
Sun
Yun-wei
YW
Department of Gastroenterology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhang
Yong-ping
YP
Qiao
Min-min
MM
Fu
Hua
H
Yuan
Yao-zong
YZ
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002908:Chronic Disease; D055513:Connective Tissue Growth Factor; D005786:Gene Expression Regulation; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D010195:Pancreatitis / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
C526316:CCN2 protein, rat; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor