title: Exposure to low doses of solar-simulated radiation induces an increase in the myeloid subtype of blood dendritic cells.
abstract: Exposure to ultraviolet radiation causes alterations of cutaneous and systemic immunity. The aim of our study was to assess the influence of low doses of solar-simulated radiation (SSR) on the phenotypes of blood dendritic cells (BDC). Healthy volunteers (94) were irradiated with a dose of 1.2 SED (standard erythema dose) of SSR for 2, 10 or 30 consecutive days. Blood samples were taken before the first exposure and 24 h after final exposure. The three main subsets of BDC were distinguished by flow cytometry: BDCA-2(+)/CD123(+)/HLA-DR(+) (plasmacytoid, PDC) and two myeloid subtypes BDCA-1(+)/CD11c(+)/HLA-DR(+) (MDC1) and BDCA-3(+)/CD32(-)/HLA-DR(+) (MDC2). The percentage of total DC was elevated in all groups by the UV exposure and was significantly increased after 2 and 30 days of irradiation (P = 0.006 and P = 0.018, respectively). A particularly distinct increase was observed in the percentage of the MDC1 after 2 and 30 days (P = 0.022 and P < 0.0001, respectively). The MDC2 showed an increase after 10 days and a subsequent significant decrease after 30 days of irradiation (P = 0.031). A significant increase in PDC was found after 2 days of irradiation (P = 0.0006). Exposure to SSR induced an increase in the percentage of BDC in healthy human individuals, especially apparent in the MDC1 subtype.
authors.affiliation: Department of Dermatology, Medical University of Lodz, Lodz, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Narbutt
authors.forename: M
authors.initials: M
authors.lastname: Skibinska
authors.forename: A
authors.initials: A
authors.lastname: Lesiak
authors.forename: A
authors.initials: A
authors.lastname: Wozniacka
authors.forename: A
authors.initials: A
authors.lastname: Sysa-Jedrzejowska
authors.forename: B
authors.initials: B
authors.lastname: Cebula
authors.forename: T
authors.initials: T
authors.lastname: Robak
authors.forename: P
authors.initials: P
authors.lastname: Smolewski
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D003713:Dendritic Cells / Q000145:classification* / Q000276:immunology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D014466:Ultraviolet Rays
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens