Dual role for phenylalanine 178 during catalysis by aristolochene synthase.
A mutant of aristolochene synthase, in which Phe 178 was replaced by Val, produced significant amounts of alpha-and beta-farnesene as well as alpha and beta-selinene and selina-4,11-diene, suggesting that Phe 178 is involved in the stabilisation of transition states preceding germacrene A and following eudesmane cation.
Forcat
Silvia
S
School of Chemistry, University of Birmingham, Edgbaston, Birmingham, UKB15 2TT.
Allemann
Rudolf K
RK
D019943:Amino Acid Substitution; D002384:Catalysis; D003500:Cyclization; D007535:Isomerases / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010649:Phenylalanine / Q000737:chemistry*; D011106:Polyisoprenyl Phosphates / Q000737:chemistry*; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry*
D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C471077:germacrene A; D010649:Phenylalanine; C004808:farnesyl pyrophosphate; D007535:Isomerases; C060050:aristolochene synthase