Intravenous apyrase administration reduces arterial thrombosis in a rabbit model of endothelial denudation in vivo.
The role of adenine nucleotides on vascular and platelet functions has long been established. Apyrase (CD39) takes part of a family of ecto-enzymes that hydrolyze adenosine diphosphate and adenosine triphosphate. The participation of apyrase in the thromboregulatory system is under study. An in vivo experimental model of acute arterial thrombosis was used to test the hypothesis that administering a soluble form of potato apyrase could prevent thrombus formation. Twenty-five white New Zealand male rabbits suffered balloon aortic endothelium denudation and, after 15 days, they were submitted to a thrombosis-triggering protocol with a procoagulant (Russel's viper venom) and epinephrine. After the thrombosis-triggering protocol, 12 animals received two soluble apyrase administrations intravenously (with 90 min intervals), while 13 control animals received no apyrase. Three hours after the triggering protocol, the animals were killed and the rate and area of arterial thrombosis were analyzed. The rate of thrombosis in the apyrase group was significantly lower than that of the control group (16.7 versus 69%, respectively; P = 0.015), as was the area of thrombosis (1.7 +/- 4.3 versus 21.7 +/- 37.4 mm2, respectively; P = 0.008). Our results confirm that apyrase participates in homeostasis through a potent anti-thrombotic effect.
Costa
Andry F
AF
Programa de pós-graduação em Cardiologia, Serviço de Cardiologia, Hospital de Clínicas de Porto Alegre, UFRGS, RS, Brazil. andrycosta@brturbo.com
Gamermann
Patrícia W
PW
Picon
Paula X
PX
Mosmann
Marcos P
MP
Kettlun
Ana Maria
AM
Valenzuela
Maria Antonieta
MA
Sarkis
João J F
JJ
Battastini
Ana Maria O
AM
Picon
Paulo D
PD
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001081:Apyrase / Q000008:administration & dosage* / Q000378:metabolism; D002404:Catheterization; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D004837:Epinephrine / Q000008:administration & dosage; D007275:Injections, Intravenous; D010940:Plant Proteins / Q000008:administration & dosage* / Q000378:metabolism; D011817:Rabbits; D011198:Solanum tuberosum / Q000201:enzymology; D013927:Thrombosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014662:Vasoconstrictor Agents / Q000008:administration & dosage; D014757:Viper Venoms / Q000008:administration & dosage
D000227:Adenine Nucleotides; D010940:Plant Proteins; D014662:Vasoconstrictor Agents; D014757:Viper Venoms; D001081:Apyrase; D004837:Epinephrine