title: Association of methylenetetrahydrofolate reductase T677 allele with early development of chronic allograft nephropathy.
abstract: OBJECTIVE
  The aim of this study was to evaluate the role of genetic polymorphisms on the development of chronic allograft nephropathy (CAN).
  
  
  METHODS
  Using the polymerase chain reaction (PCR), polymorphisms of methylenetetrahydrofolate reductase (MTHFR C677T), interleukin-6 (IL-6 G-174C) and CD14 (C-260T) were evaluated in 92 kidney transplant recipients with stable renal graft function and no signs of acute rejection in a protocol that included graft biopsy at 12 months after kidney transplantation. A normal population sample (n = 365) was also included. Multivariate analysis was used to evaluate the effect of different variables on the CAN appearance.
  
  
  RESULTS
  There were no differences in alleles and genotypes distribution between transplant group and normal population sample. The CAN+ group (n = 69) significantly differed from CAN- (n = 23) in both MTHFR (P < 0.05) and IL-6 (P < 0.01) genotype distribution. Using logistic regression multivariate analysis, MTHFR T677 allele (OR: 3.91, CI: 1.11-13.8; P < 0.05), patient age (OR: 0.94, CI: 0.88-0.98; P < 0.01) and proteinuria (OR: 3.63, CI: 1.25-10.6; P < 0.05) were associated with CAN. Although the IL-6 G-174 allele was shown to be associated with CAN development in univariate analysis (P < 0.01), the multivariate analysis did not show an association. There was no relation between CD14 gene polymorphism and CAN.
  
  
  CONCLUSIONS
  The MTHFR T677 allele is associated with the presence of CAN in kidney graft biopsies 12 months after transplantation.
authors.affiliation: Department of Nephrology, Institute for Clinical and Experimental Medicine, 14021, Prague 4, Czech Republic. ondrej.viklicky@medicon.cz
authors.forename: Ondrej
authors.initials: O
authors.lastname: Viklický
authors.forename: Jaroslav A
authors.initials: JA
authors.lastname: Hubácek
authors.forename: Jan
authors.initials: J
authors.lastname: Kvasnicka
authors.forename: Ivo
authors.initials: I
authors.lastname: Matl
authors.forename: Ludek
authors.initials: L
authors.lastname: Voska
authors.forename: Jelena
authors.initials: J
authors.lastname: Skibová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Teplan
authors.forename: Stefan
authors.initials: S
authors.lastname: Vítko
mesh_terms: D000483:Alleles; D002908:Chronic Disease; D005260:Female; D020022:Genetic Predisposition to Disease; D006086:Graft vs Host Disease / Q000201:enzymology / Q000235:genetics; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D007674:Kidney Diseases / Q000201:enzymology / Q000235:genetics*; D016030:Kidney Transplantation; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011507:Proteinuria; D014184:Transplantation, Homologous
chemical_list: D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)