title: Effects of genetic and nutritional factors on bone mineral density in young adults.
abstract: To estimate the genetic and dietary factors influencing bone mineral density (BMD) in young adults, a total of 53 healthy volunteers (HV) (age 20.89+/-1.34), from whom informed consent was obtained, answered a questionnaire on dietary factors and had DNA from peripheral blood mononuclear cells analyzed for single nucleotide polymorphisms (SNPs) for vitamin (Vit) D receptor (VDR), estrogen receptor alpha (ERalpha), interleukin 1 receptor antagonist (IL1RA), and apolipoprotein E (ApoE) genes. Daily intakes of Vit C, fiber, soybean and related foods, and green and yellow vegetables showed a correlation with % BMD. In addition, Vit B2 as well as Vit C, and vegetables were identified as important factors for BMD by Stepwise regression analysis. Among the SNPs analyzed, the B+ type of the VDR gene tended to be associated with a lower BMD, and pp type of the ER gene digested by the PvuII enzyme in females indicated a significantly lower BMD than that in males. In addition, these SNPs were also identified by factor analysis to be associated with BMD. These results suggested that a complex array of genetic factors, such as two or more SNPs or SNPs and gender, may be important to BMD.
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan. takemi@med.kawasaki-m.ac.jp
authors.forename: Takemi
authors.initials: T
authors.lastname: Otsuki
authors.forename: Haruko
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Emiko
authors.initials: E
authors.lastname: Hatta
authors.forename: Tamayo
authors.initials: T
authors.lastname: Hatayama
authors.forename: Satomi
authors.initials: S
authors.lastname: Hatada
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Miura
authors.forename: Akiko
authors.initials: A
authors.lastname: Takata-Tomokuni
authors.forename: Fuminori
authors.initials: F
authors.lastname: Hyodoh
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Tomomitsu
authors.forename: Masao
authors.initials: M
authors.lastname: Fukunaga
authors.forename: Hironobu
authors.initials: H
authors.lastname: Katsuyama
mesh_terms: D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics; D015519:Bone Density / Q000235:genetics* / Q000502:physiology*; D004032:Diet; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D016014:Linear Models; D008297:Male; D009747:Nutritional Physiological Phenomena; D009752:Nutritional Status / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018167:Receptors, Calcitriol / Q000235:genetics; D012727:Sex Characteristics; D012795:Sialoglycoproteins / Q000235:genetics; D011795:Surveys and Questionnaires
chemical_list: D001057:Apolipoproteins E; D047628:Estrogen Receptor alpha; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D018167:Receptors, Calcitriol; D012795:Sialoglycoproteins