Effect of icodextrin on transperitoneal uric acid and albumin transport in vitro.
In the present study, we compared the influence of icodextrin (2 g/dL) on uric acid (20 mg/dL) and albumin (1 g/dL) transfer through isolated rabbit parietal peritoneum. In separate series of experiments, we used a mathematical model to calculate the transport rate of solutes from the interstitial to the mesothelial side of peritoneal membrane (I --> M) and in the opposite direction (M --> I) before and after introduction of glucose polymer. In control conditions, the rates of uric acid and albumin passage across the rabbit peritoneum remained constant. Mean values [x +/- standard error of the mean (SEM)] of the diffusive permeability coefficient P were 1.936 +/- 0.324 (I --> M) and 2.078 +/- 0.186 (M --> I) for uric acid and 0.341 +/- 0.066 (I --> M) and 0.389 +/- 0.084 (M --> I) for albumin (all x10(-4) cm/s), respectively. The introduction of glucose polymer into the experimental system did not alter the I --> M transport of either solute; but, in the opposite direction, it caused a 17% decline in the P for uric acid (p < 0.05), and an increase of 77% in the same parameter for albumin (p < 0.03). Hence, I --> M transfer dominated M --> I transfer of uric acid (p < 0.04). In contrast, in albumin transport, M --> I was higher than I --> M (p < 0.02). We conclude that, in vitro, icodextrin modifies the diffusive permeability of the peritoneum and induces transport asymmetry for some small and large solutes.
Grzelak
Teresa
T
Department of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland. tgrzelak@amp.edu.pl
Szary
Beata
B
Czyzewska
Krystyna
K
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D046508:Culture Techniques; D005936:Glucans / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D015312:Hemodialysis Solutions / Q000494:pharmacology*; D000077607:Icodextrin; D008297:Male; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D014527:Uric Acid / Q000378:metabolism*
D000418:Albumins; D005936:Glucans; D015312:Hemodialysis Solutions; D014527:Uric Acid; D000077607:Icodextrin; D005947:Glucose