Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400T and relatives as Burkholderia xenovorans sp. nov.
Strain LB400T is the best-studied polychlorinated biphenyl (PCB) degrader. This organism has previously been allocated in the genus Burkholderia, since its 16S rRNA gene sequence shows 98.6 % sequence similarity to the type strains of Burkholderia graminis and Burkholderia terricola. A polyphasic study was undertaken to clarify the actual taxonomic position of this biotechnologically important organism and of two strains, one recovered from a blood culture vial and one from a coffee plant rhizosphere, both of which resembled strain LB400T in their whole-cell protein patterns. DNA-DNA hybridization experiments revealed that the three strains represented a single novel species, for which the name Burkholderia xenovorans sp. nov. is proposed. Strains of this novel species can be differentiated phenotypically from nearly all other Burkholderia species by their inability to assimilate L-arabinose. The whole-cell fatty acid profile of B. xenovorans strains is consistent with their classification in the genus Burkholderia, with 18 : 1omega7c, 16 : 1omega7c, 16 : 0, 14 : 0 3OH, 16 : 0 3OH, 17 : 0 cyclo and 14 : 0 being the most abundant fatty acids. The G + C content of the species varies between 62.4 and 62.9 mol%. The type strain of B. xenovorans is LB400T (= LMG 21463T = CCUG 46959T = NRRL B-18064T).
Goris
Johan
J
Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Gent, Belgium.
De Vos
Paul
P
Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Gent, Belgium.
Caballero-Mellado
Jesús
J
P. de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Park
Joonhong
J
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-413 46 Göteborg, Sweden.
Quensen
John F
JF
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
Tiedje
James M
JM
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
Vandamme
Peter
P
Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Gent, Belgium.
D001089:Arabinose / Q000378:metabolism; D001482:Base Composition; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D001769:Blood / Q000382:microbiology; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019121:Burkholderia Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006801:Humans; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D001089:Arabinose; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D005227:Fatty Acids; D011078:Polychlorinated Biphenyls; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C010574:biphenyl