Lycopene suppresses the lipopolysaccharide-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and nuclear factor-kappaB.
Dendritic cells (DC) are the most potent of antigen-presenting cells. The most important function of DC is to initiate the immune response by presenting antigens to naïve T lymphocytes. Currently, little is known about the basic action of lycopene in murine bone marrow (BM)-derived DC. In the present study, we have revealed that lycopene significantly attenuates the phenotypic and functional maturation of murine BM-DC, especially in lipopolysaccharide-induced DC maturation. We found that lycopene down-regulates the expression of costimulatory molecules (CD80 and CD86) and major histocompatibility complex type II molecules. We also determined that lycopene-treated DC were poor stimulators of naïve allogeneic T-cell proliferation and induced lower levels of interleukin-2 in responding T cells. They also exhibited impaired interleukin-12 production. Additionally, lycopene was able to inhibit mitogen-activated protein kinases, such as ERK1/2, p38 and JNK, and the transcription factor, nuclear factor-kappaB. Assessment of the in vivo effects of lycopene may reveal an inability to induce a normal cell-mediated immune response, despite the ability of the cells to migrate to the spleen. This data provides new insight into the immunopharmacology of lycopene and suggests a novel approach to the manipulation of DC for therapeutic application.
Kim
Gi-Young
GY
Department of Microbiology, Pusan National University College of Medicine, Pusan, South Korea.
Kim
Jong-Hyun
JH
Ahn
Soon-Cheol
SC
Lee
Hee-Jeong
HJ
Moon
Dong-Oh
DO
Lee
Chang-Min
CM
Park
Yeong-Min
YM
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000975:Antioxidants / Q000502:physiology*; D002338:Carotenoids / Q000008:administration & dosage / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D004705:Endocytosis / Q000276:immunology; D005802:Genes, MHC Class II / Q000235:genetics / Q000276:immunology; D007274:Injections, Intraperitoneal; D018664:Interleukin-12 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology; D000077276:Lycopene; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D000975:Antioxidants; D007376:Interleukin-2; D008070:Lipopolysaccharides; D016328:NF-kappa B; D018664:Interleukin-12; D002338:Carotenoids; D020928:Mitogen-Activated Protein Kinases; D000077276:Lycopene