Change with donor age in the degradation rate of endogenous proteins of mouse hepatocytes in primary culture.
The degradation rate of [(3)H]leucine labeled intracellular proteins was compared in hepatocytes derived from young (4-7-month-old) and aged (23-month-old) mice. Average half-lives of long-lived proteins were 40 h and 65 h in the cells from young and old animals, respectively. This observation confirms our earlier results that half-lives of foreign proteins introduced into the cells from old mice are 50-60% longer than those of younger counterparts (Ishigami and Goto, 1988, Mech. Ageing Dev., 46, 125-133; Ishigami and Goto, 1990, Arch. Biochem. Biophys., 277, 189-195).
Ikeda
T
T
Department of Biochemistry, School of Pharmaceutical Science, Toho University, Miyama, Funabashi, Chiba, 274 Japan.
Ishigamia
A
A
Anzai
K
K
Goto
S
S