[Study on the relationship between Fas expression and apoptosis in oral squamous cell carcinoma].
OBJECTIVE
To investigate the relationship of Fas mRNA and protein expression and apoptosis in human oral squamous cell carcinoma.


METHODS
Northern blot and flow cytometry (TUNEL method) were used to detect the expression of Fas mRNA and Fas protein, cell cycle and apoptotic level in oral squamous cell carcinoma. The relationship between Fas gene expression and OSCC apoptosis was analyzed statistically.


RESULTS
Fas mRNA and protein could be detected in all five normal oral mucosa specimens. There was positive correlation between expression of Fas mRNA/protein and cell differentiation as well as apoptosis in OSCC (P < 0.005).


CONCLUSIONS
The expression of Fas gene was highly correlated with the differentiation and apoptosis in OSCC.
Wang
Jian-guang
JG
Dept. of Oral and Cranio-Maxillofacial Surgery, the Second Affiliated Hospital, Guanzhou 510120, China.
Huang
Hong-zhang
HZ
Pan
Chao-bin
CB
Hou
Jin-song
JS
Li
Jin-song
JS
Cheng
Bin
B
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
C491901:FAS protein, human; D019014:fas Receptor