title: Pregnancy and bovine somatotropin in nonlactating dairy cows: II. Endometrial gene expression related to maintenance of pregnancy.
abstract: The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bST-treated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor alpha (ERalpha) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERalpha protein, and bST stimulated an increase in ERalpha protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERalpha protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P cows than in P cows. In the stromal layer of the endometrium, bST decreased PR protein abundance in C and P cows. The PGHS-2 protein was localized exclusively in the luminal epithelium cells of endometrium and was increased in P cows. In conclusion, distinctly different mRNA and protein responses were detected between C and P cows related to prostaglandin biosynthesis, and bST-induced changes may potentially impact mechanisms associated with maintenance of pregnancy in nonlactating cows.
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville 32611-0910, USA.
authors.forename: A
authors.initials: A
authors.lastname: Guzeloglu
authors.forename: T R
authors.initials: TR
authors.lastname: Bilby
authors.forename: A
authors.initials: A
authors.lastname: Meikle
authors.forename: S
authors.initials: S
authors.lastname: Kamimura
authors.forename: A
authors.initials: A
authors.lastname: Kowalski
authors.forename: F
authors.initials: F
authors.lastname: Michel
authors.forename: L A
authors.initials: LA
authors.lastname: MacLaren
authors.forename: W W
authors.initials: WW
authors.lastname: Thatcher
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000378:metabolism*; D015237:Dinoprost / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D013006:Growth Hormone / Q000494:pharmacology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000235:genetics; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D011247:Pregnancy; D011255:Pregnancy Maintenance / Q000235:genetics*; D000071876:Prostaglandin-E Synthases; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018045:Receptors, Oxytocin / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D011980:Receptors, Progesterone; C073294:growth hormone, bovine; D013006:Growth Hormone; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D011451:Prostaglandin-Endoperoxide Synthases; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone