Bioremediation of quinoline-contaminated soil using bioaugmentation in slurry-phase reactor.
OBJECTIVE
To investigate the possibility of using bioaugmentation as a strategy for remediating quinoline-contaminated soil.


METHODS
Microorganisms were introduced to the soil to assess the feasibility of enhancing the removal of quinoline from quinoline-contaminated soil. Slurry-phase reactor was used to investigate the bioremediation of quinoline-contaminated soil. HPLC (Hewlett-Packard model 5050 with an UV detector) was used for analysis of quinoline concentration.


RESULTS
The biodegradation rate of quinoline was increased through the introduction of Burkholderia pickettii. Quinoline, at a concentration of 1 mg/g soil, could be removed completely within 6 and 8 hours with and without combined effect of indigenous microbes, respectively. Although the indigenous microbes alone had no quinoline-degrading ability, they cooperated with the introduced quinoline-degrader to remove quinoline more quickly than the introduced microbes alone. Bioaugmentaion process was accelerated by the increase of inoculum size and bio-stimulation. The ratio of water to soil in slurry had no significant impact on bioremediation results.


CONCLUSIONS
Bioaugmetation is an effective way for bioremediation of quinoline-contaminated soil.
Wang
Jian-Long
JL
Laboratory of Environmental Technology, Inet, Tsinghua University, Beijing 100084, China. wangjl@tsinghua.edu.cn
Mao
Ze-Yu
ZY
Han
Li-Ping
LP
Qian
Yi
Y
D001673:Biodegradation, Environmental; D019149:Bioreactors; D019117:Burkholderia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D011804:Quinolines; D012722:Sewage; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants
D011804:Quinolines; D012722:Sewage; D012987:Soil; D012989:Soil Pollutants; C037219:quinoline