title: AmpC beta-lactamase in an Escherichia coli clinical isolate confers resistance to expanded-spectrum cephalosporins.
abstract: Cloning, sequencing, and biochemical analysis identified a novel AmpC-type beta-lactamase conferring resistance to extended-spectrum cephalosporins in an Escherichia coli clinical isolate. This enzyme, exhibiting 14 amino acid substitutions compared to a reference AmpC cephalosporinase of E. coli, hydrolyzed ceftazidime and cefepime significantly.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. nordmann.patrice@bct.ap-hop-paris.fr
authors.forename: Hedi
authors.initials: H
authors.lastname: Mammeri
authors.forename: Hasan
authors.initials: H
authors.lastname: Nazic
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Sophie
authors.initials: S
authors.lastname: Léotard
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D000077723:Cefepime; D002442:Ceftazidime / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D004269:DNA, Bacterial; D000077723:Cefepime; D002442:Ceftazidime; C048795:AmpC beta-lactamases; D001618:beta-Lactamases