title: The dMRP/CG6214 gene of Drosophila is evolutionarily and functionally related to the human multidrug resistance-associated protein family.
abstract: ATP-binding cassette (ABC) transporters are involved in the transport of substrates across biological membranes and are essential for many cellular processes. Of the fifty-six Drosophila ABC transporter genes only white, brown, scarlet, E23 and Atet have been studied in detail. Phylogenetic analyses identify the Drosophila gene dMRP/CG6214 as an orthologue to the human multidrug-resistance associated proteins MRP1, MRP2, MRP3 and MRP6. To study evolutionarily conserved roles of MRPs we have initiated a characterization of dMRP. In situ hybridization and Northern analysis indicate that dMRP is expressed throughout development and appears to be head enriched in adults. Functional studies indicate that DMRP is capable of transporting a known MRP1 substrate and establishes DMRP as a high capacity ATP-dependent, vanadate-sensitive organic anion transporter.
authors.affiliation: Cell and Molecular Biology, University of Hawaii, Honolulu, HI 96822, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Tarnay
authors.forename: F
authors.initials: F
authors.lastname: Szeri
authors.forename: A
authors.initials: A
authors.lastname: Iliás
authors.forename: T
authors.initials: T
authors.lastname: Annilo
authors.forename: C
authors.initials: C
authors.lastname: Sung
authors.forename: O
authors.initials: O
authors.lastname: Le Saux
authors.forename: A
authors.initials: A
authors.lastname: Váradi
authors.forename: M
authors.initials: M
authors.lastname: Dean
authors.forename: C D
authors.initials: CD
authors.lastname: Boyd
authors.forename: S
authors.initials: S
authors.lastname: Robinow
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D016000:Cluster Analysis; D017931:DNA Primers; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011487:Protein Conformation; D013045:Species Specificity
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D029721:Drosophila Proteins; D008565:Membrane Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C495706:MRP protein, Drosophila