A polyhydroxybutyrate-producing Pseudomonas sp. isolated from Antarctic environments with high stress resistance.
Pseudomonas sp. 14-3, a strain that accumulates large quantities of polyhydroxybutyrate (PHB) when grown on octanoate, was isolated from Antarctic environments. This isolate was characterized on the basis of phenotypic features and partial sequencing of its 16S ribosomal RNA gene. Pseudomonas sp. 14-3 showed increased tolerance to both thermal and oxidative stress compared with three other Pseudomonas species. Stress tolerance of Pseudomonas sp. 14-3 was analyzed in polyhydroxyalkanoate accumulating and non-accumulating conditions, and increased levels of stress resistance were observed when PHB was produced. Pseudomonas sp. 14-3 was isolated from Antarctic regions, a habitat normally exposed to extreme conditions. An association between high PHB accumulation and high stress resistance in bacteria adapted to extreme environments is suggested.
Ayub
Nicolás D
ND
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428-Buenos Aires, Argentina.
Pettinari
M Julia
MJ
Ruiz
Jimena A
JA
López
Nancy I
NI
D000864:Antarctic Regions; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006358:Hot Temperature; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010641:Phenotype; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology; D000094122:Polyhydroxybutyrates
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006885:Hydroxybutyrates; D011091:Polyesters; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates