title: Synthetic molecules as antibody replacements.
abstract: Antibodies are by far the most versatile, valuable, and widely used protein-binding agents. They are essential tools in biological research and are increasingly being developed as therapeutic reagents. However, antibodies have a number of practical limitations, and it would be desirable in many applications to replace them with simpler, more robust synthetic molecules. Unfortunately, synthetic protein-binding agents rarely exhibit the high affinity and specificity typical of a good antibody. This article reviews efforts to overcome these limitations and to develop a facile, high-throughput methodology for the isolation of synthetic protein ligands with antibody-like binding characteristics.
authors.affiliation: Center for Biomedical Inventions, Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-8573, USA. Thomas.Kodadek@utsouthwestern.edu
authors.forename: Thomas
authors.initials: T
authors.lastname: Kodadek
authors.forename: M Muralidhar
authors.initials: MM
authors.lastname: Reddy
authors.forename: Hernando J
authors.initials: HJ
authors.lastname: Olivos
authors.forename: Kiran
authors.initials: K
authors.lastname: Bachhawat-Sikder
authors.forename: Prasanna G
authors.initials: PG
authors.lastname: Alluri
mesh_terms: D018033:Antibodies, Bispecific / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity; D040761:Biomimetic Materials / Q000737:chemistry*; D018189:Immunomagnetic Separation; D007700:Kinetics; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D018033:Antibodies, Bispecific; D019151:Peptide Library