Genomic instability in hepatocellular carcinoma revealed by using the random amplified polymorphic DNA method.
OBJECTIVE
To investigate the genomic instability and their association with clinicopathological characteristics in hepatocellular carcinoma (HCC).


METHODS
DNA isolated from tumors and corresponding non-cancerous liver tissues of 56 patients with HCC was amplified with ten random 10-mer arbitrary primers by the random amplified polymorphic DNA (RAPD) method.


RESULTS
All the cases of HCC were demonstrated to have genomic instability by at least one primer. The incidence of genomic instability ranged from 20 to 70% in each case, and 17.9-50% in each primer. Serum AFP concentration, HBV infection, tumor size, histological grade, tumor capsule invasion, as well as intrahepatic metastasis were associated with the genomic instability on certain primers.


CONCLUSIONS
Genomic instability is a frequent event in HCC. The RAPD is an effective method for the identification and analysis of genomic instability in HCC, and it may provide new information for further evaluating the molecular mechanism of hepatocarcinogenesis.
Zhang
Shu-Hui
SH
Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
Cong
Wen-Ming
WM
Xian
Zhi-Hong
ZH
Dong
Hui
H
Wu
Meng-Chao
MC
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D042822:Genomic Instability; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D019105:Random Amplified Polymorphic DNA Technique; D000509:alpha-Fetoproteins / Q000032:analysis
D006514:Hepatitis B Surface Antigens; D000509:alpha-Fetoproteins