title: Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration.
abstract: Syndecan-3 and syndecan-4 function as coreceptors for tyrosine kinases and in cell adhesion. Syndecan-3(-/-) mice exhibit a novel form of muscular dystrophy characterized by impaired locomotion, fibrosis, and hyperplasia of myonuclei and satellite cells. Explanted syndecan-3(-/-) satellite cells mislocalize MyoD, differentiate aberrantly, and exhibit a general increase in overall tyrosine phosphorylation. Following induced regeneration, the hyperplastic phenotype is recapitulated. While there are fewer apparent defects in syndecan-4(-/-) muscle, explanted satellite cells are deficient in activation, proliferation, MyoD expression, myotube fusion, and differentiation. Further, syndecan-4(-/-) satellite cells fail to reconstitute damaged muscle, suggesting a unique requirement for syndecan-4 in satellite cell function.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado 80309, USA.
authors.forename: D D W
authors.initials: DD
authors.lastname: Cornelison
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Wilcox-Adelman
authors.forename: Paul F
authors.initials: PF
authors.lastname: Goetinck
authors.forename: Heikki
authors.initials: H
authors.lastname: Rauvala
authors.forename: Alan C
authors.initials: AC
authors.lastname: Rapraeger
authors.forename: Bradley B
authors.initials: BB
authors.lastname: Olwin
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000473:pathology / Q000502:physiology; D018482:Muscle, Skeletal / Q000254:growth & development / Q000502:physiology*; D009136:Muscular Dystrophies / Q000235:genetics / Q000503:physiopathology; D017570:MyoD Protein / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000502:physiology*; D012038:Regeneration / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000473:pathology; D053670:Syndecan-3; D053671:Syndecan-4
chemical_list: D008562:Membrane Glycoproteins; D017570:MyoD Protein; D011509:Proteoglycans; C508302:Sdc3 protein, mouse; C508306:Sdc4 protein, mouse; D053670:Syndecan-3; D053671:Syndecan-4