Mucin-1 is expressed on dendritic cells, both in vitro and in vivo.
Dendritic cells (DCs) are the best professional antigen-presenting cells to stimulate cytotoxic as well as T helper cells and are therefore appropriate candidates for establishing immunotherapy. The concept of our vaccination program is to introduce the tumor-associated antigen mucin-1 (MUC1) into DCs. Analysis of immature and mature DCs--before transducing the antigen MUC1--already demonstrated expression of MUC1 on in vitro monocyte-derived DCs upon maturation. Different culture methods as well as maturation cocktails showed similar results concerning the upregulation of MUC1 expression. Furthermore, we studied the expression of MUC1 on DCs in vivo. No MUC1 expression was found on blood DCs, or on thymic or tonsil DCs. On the other hand, synovial fluid from patients with arthritis contained DCs that were found to express MUC1. This study shows for the first time that the tumor-associated antigen MUC1 is expressed on in vivo DCs. We further show that MUC1 is also expressed on in vitro cultured bone marrow-derived DCs of human MUC1 transgenic mice, supporting the relevance of this mouse model to the human situation. The observation that MUC1 is present on in vivo DCs suggests a functional role, but this physiological function remains to be elucidated.
Cloosen
Silvie
S
Department of Internal Medicine, Division of Hemato-Oncology, University Hospital Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands. S.Cloosen@immuno.unimaas.nl
Thio
Marco
M
Vanclée
Ariane
A
van Leeuwen
Ellen B M
EB
Senden-Gijsbers
Birgit L M G
BL
Oving
Ellis B H
EB
Germeraad
Wilfred T V
WT
Bos
Gerard M J
GM
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D016219:Immunotherapy, Adoptive; D051379:Mice; D008822:Mice, Transgenic; D018396:Mucin-1 / Q000235:genetics / Q000276:immunology*; D009369:Neoplasms / Q000473:pathology / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D018396:Mucin-1