title: Ostreolysin, a pore-forming protein from the oyster mushroom, interacts specifically with membrane cholesterol-rich lipid domains.
abstract: Ostreolysin, a 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoylphosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase.
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepcić
authors.forename: Sabina
authors.initials: S
authors.lastname: Berne
authors.forename: Katja
authors.initials: K
authors.lastname: Rebolj
authors.forename: Urska
authors.initials: U
authors.lastname: Batista
authors.forename: Ana
authors.initials: A
authors.lastname: Plemenitas
authors.forename: Marjeta
authors.initials: M
authors.lastname: Sentjurc
authors.forename: Peter
authors.initials: P
authors.lastname: Macek
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002784:Cholesterol / Q000378:metabolism*; D006224:Cricetinae; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D020076:Pleurotus / Q000737:chemistry*; D011485:Protein Binding; D013109:Sphingomyelins / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D013109:Sphingomyelins; C461280:ostreolysin; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; C007740:fluorexon