title: Induction of Mycobacterium avium proteins upon infection of human macrophages.
abstract: Induction of Mycobacterium avium proteins labelled with [35S]methionine and mRNAs upon infection of the human macrophage cell line THP-1 was investigated by two-dimensional gel electrophoresis-mass spectrometry and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. M. avium overexpressed proteins within the macrophages that are involved in fatty acids metabolism (FadE2, FixA), cell wall synthesis (KasA), and protein synthesis (EF-tu). The correlation of differential protein and mRNA expression varied between good and no correlation. Overall, these four proteins may be involved in the adaptation and survival of M. avium within human macrophages.
authors.affiliation: Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Roma, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Brunori
authors.forename: Federico
authors.initials: F
authors.lastname: Giannoni
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.forename: Sabrina
authors.initials: S
authors.lastname: Liberatori
authors.forename: Cristiane
authors.initials: C
authors.lastname: Frota
authors.forename: Peter
authors.initials: P
authors.lastname: Jenner
authors.forename: Ove Fredrik
authors.initials: OF
authors.lastname: Thoresen
authors.forename: Graziella
authors.initials: G
authors.lastname: Orefici
authors.forename: Lanfranco
authors.initials: L
authors.lastname: Fattorini
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D009164:Mycobacterium Infections / Q000378:metabolism; D009162:Mycobacterium avium / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D010444:Peptide Elongation Factor Tu / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D010444:Peptide Elongation Factor Tu