Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai.
We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.
Lee
Soo-Ung
SU
Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Repubic of Korea. shuh@hallym.ac.kr
Huh
Sun
S
Sohn
Woon-Mok
WM
Chai
Jong-Yil
JY
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D006578:Heterophyidae / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014201:Trematode Infections / Q000469:parasitology*
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D003576:Electron Transport Complex IV