High-performance liquid chromatographic analysis of chlorophylls, pheophytins and carotenoids in virgin olive oils: chemometric approach to variety classification.
This work evaluate the possibility to get from the quali-quantitative determination of the pigments contained in monovarietal olive oils (chlorophylls, pheophytins and carotenoids) and from the multivariate statistical analysis of these measures, parameters able to distinguish within the cultivars. The chemometric variables used have concurred to obtain preliminary interesting results. Liquid-phase distribution and solid-phase extraction/purification procedures has been compared: recoveries for both are resulted higher than 94% for all the pigment classes and the R.S.D. values were below 10%. HPLC analysis, allowing the simultaneous pigment determination, and fluorescence detection, allowing a better green pigments measure (detection limits from 5 to 80ppb), are revealed a fundamental solution.
Cichelli
Angelo
A
Dipartimento di Scienze, Università degli Studi Gabriele d'Annunzio, Viale Pindaro 42, 65015 Pescara, Italy.
Pertesana
Gian Pietro
GP
D002338:Carotenoids / Q000032:analysis*; D002734:Chlorophyll / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D000069463:Olive Oil; D010674:Pheophytins / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results
D000069463:Olive Oil; D010674:Pheophytins; D010938:Plant Oils; D002734:Chlorophyll; D002338:Carotenoids