title: A new VHR dual-specificity protein tyrosine phosphatase inhibitor from Dendrobium moniliforme.
abstract: Bioassay-guided fractionation of the EtOAc-soluble extract of Dendrobium moniliforme afforded a new phenanthraquinone-type metabolite, 7-hydroxy-5,6-dimethoxy-1,4-phenanthrenequinone ( 1), along with the previously reported 5-hydroxy-3,7-dimethoxy-1,4-phenanthrenequinone ( 2). The structures of the compounds were identified mainly on the basis of MS and NMR data. Compound 1 inhibited VHR dual-specificity protein tyrosine phosphatase (DS-PTPase) activity in a dose-dependent manner, displaying an IC (50) value of 3.0 +/- 0.2 microM.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yusong, Taejeon 305-333, South Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Bae
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
authors.forename: Won Keun
authors.initials: WK
authors.lastname: Oh
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Kim
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Cheon Bae
authors.initials: CB
authors.lastname: Sohn
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
mesh_terms: D031667:Dendrobium; D004305:Dose-Response Relationship, Drug; D054640:Dual Specificity Phosphatase 3; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018547:Plant Stems; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; C517025:DUSP3 protein, human; D054640:Dual Specificity Phosphatase 3; D017027:Protein Tyrosine Phosphatases