title: Blood culture examinations at a community hospital without a microbiology laboratory: using an automated blood culture system and performing a Gram stain on positive culture bottles in the institution.
abstract: To elucidate the existence of microorganisms from blood culture bottles in hospitals without a microbiology laboratory, we changed the system of blood culture examinations. The Oxoid signal blood culture system and submission of all blood cultures to the clinical testing industry was used from July 2002 to December 2002 (first period). Use of the BacT/Alert system and performing of Gram stain for positive culture bottles in our institutions was conducted from January 2003 to June 2003 (latter period). A total of 210 and 193 blood cultures were processed during the first and latter periods, respectively. There were 40 (19.0%) positive cultures in the first period and 32 (16.6%) positive cultures in the latter period. The times from the specimen collection to the Gram stain result that were required were 3.8 and 1.0 days in the first period and the latter period, respectively. The times required for the final report of the blood cultures in the first period and in the latter period were 5.8 and 4.9 days, respectively. We conclude that using a continuous monitoring, automated blood culture system and performing Gram stain for positive culture bottles in institutions without microbiology laboratories may be useful for medical doctors to rapidly determine the existence of microorganisms and to begin adequate antiinfective therapy.
authors.affiliation: Department of Infectious Disease, Federation of Public Services and Affiliated Personnel Aid Associations, Takamatsu Hospital, 4-18 Tenjinmae, Takamatsu, Kagawa 760-0018, Japan. saitoh@kkr-ta-hp.gr.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Saito
authors.forename: Yoji
authors.initials: Y
authors.lastname: Aoki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mori
authors.forename: Fumikazu
authors.initials: F
authors.lastname: Kohi
mesh_terms: D001331:Automation; D016470:Bacteremia / Q000382:microbiology; D001419:Bacteria / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D016469:Fungemia / Q000382:microbiology; D005658:Fungi / Q000302:isolation & purification*; D005840:Gentian Violet; D006764:Hospitals, Community; D006801:Humans; D007755:Laboratories, Hospital; D008829:Microbiology; D010619:Phenazines; D013194:Staining and Labeling / Q000379:methods
chemical_list: D003470:Culture Media; C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet