title: Biologic, morphologic, and molecular characterisation of Neospora caninum isolates from littermate dogs.
abstract: Isolation and biologic and molecular attributes of Neospora caninum from three littermate dogs are described. Tissue cysts were confined to the brain and striated muscles. N. caninum was isolated (isolates NC-6, NC-7, and NC-8) in rodents and cell culture that had been inoculated with brain tissue from the dogs. Schizont-like stages reactive with N. caninum antibodies were seen in cell cultures seeded with bradyzoites released from Percoll-isolated N. caninum tissue cysts from the brain of one dog. Tissue cysts were infective orally to mice and gerbils, but not to cats and dogs. The isolates were also identified as N. caninum by PCR and sequence analysis.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC-East, Building 1040, 10300 Baltimore Avenue, Beltsville, MD 20705-2350, USA. jdubey@anri.barc.usda.gov
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: C
authors.initials: C
authors.lastname: Sreekumar
authors.forename: E
authors.initials: E
authors.lastname: Knickman
authors.forename: K B
authors.initials: KB
authors.lastname: Miska
authors.forename: M C B
authors.initials: MC
authors.lastname: Vianna
authors.forename: O C H
authors.initials: OC
authors.lastname: Kwok
authors.forename: D E
authors.initials: DE
authors.lastname: Hill
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
authors.forename: D S
authors.initials: DS
authors.lastname: Lindsay
authors.forename: C E
authors.initials: CE
authors.lastname: Greene
mesh_terms: D000818:Animals; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods; D001921:Brain / Q000469:parasitology*; D002415:Cats; D003048:Coccidiosis / Q000469:parasitology* / Q000635:transmission; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D017125:Genes, Protozoan; D005849:Gerbillinae; D008018:Life Cycle Stages; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018688:Neospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D013045:Species Specificity