title: High density peptide microarrays. In situ synthesis and applications.
abstract: The technologies enabling the creation of large scale, miniaturized peptide or protein microarrays are emerging. The focuses of this review are the synthesis and applications of peptide and peptidomimetic microarrays, especially the light directed parallel synthesis of individually addressable high density peptide microarrays using a novel photogenerated reagent chemistry and digital photolithography (Gao et al., 1998, J. Am. Chem. Soc. 120, 12698; Pellois et al. 2002, Nat. Biotechnol. 20, 922). Concepts related to the synthesis are discussed, such as the reactions of photogenerated acids in the deprotection step of peptide synthesis or oligonucleotide synthesis, and the applications of high density peptide chips in antibody binding assays are discussed. Peptide chips provide versatile tools for probing antigen-antibody, protein-protein, peptide-ligand interactions and are basic components for miniaturization, automation, and system integration in research and clinical diagnosis applications.
authors.affiliation: Department of Chemistry, University of Houston, Houston, TX 77004-5003, USA. xgao@uh.edu
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Gao
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Pellois
authors.forename: Younghwa
authors.initials: Y
authors.lastname: Na
authors.forename: Younkee
authors.initials: Y
authors.lastname: Kim
authors.forename: Erdogan
authors.initials: E
authors.lastname: Gulari
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Zhou
mesh_terms: D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis
chemical_list: D019151:Peptide Library; D010455:Peptides