Dual 13C, 15N labelling of terrestrial slugs (Deroceras reticulatum).
A simple, rapid and cost-effective laboratory method is described for labelling terrestrial slugs simultaneously with 13C and 15N. Slugs (Deroceras reticulatum) were provided with a mixture of [U-13C6]glucose, 15N-enriched lettuce powder, and wheat bran. Assimilation efficiencies for 13C (24.2%) and 15N (27.4%) were not affected by feeding regimes offering ad libitum or restricted access to unlabelled food during the labelling period. Body tissue was significantly more highly enriched in 13C but significantly less in 15N than cutaneous mucus after 15 days.
Dyckmans
Jens
J
Department of Environmental Resource Management, Faculty of Agriculture, University College Dublin, Belfield, Ireland.
Schmidt
Olaf
O
D000821:Animal Feed; D000818:Animals; D002247:Carbon Isotopes / Q000032:analysis*; D007553:Isotope Labeling / Q000379:methods*; D008974:Mollusca / Q000737:chemistry / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism; D009587:Nitrogen Isotopes / Q000032:analysis*; D013997:Time Factors
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes