title: Evaluation of DNA damage in mice topically exposed to total particulate matter from mainstream and sidestream smoke from cigarettes and bidis.
abstract: The genotoxic potential of total particulate matter (TPM) from mainstream smoke (MS) and sidestream smoke (SS) of Indian smoking products, namely cigarettes and bidis, as well as a brand of US cigarettes, was studied by determining the levels of bulky aromatic DNA adducts in mouse tissues. TPM from MS or SS of various smoking products [equal weights (2.5 mg) or the amount derived from equal (0.25) cigarette/bidi] was applied topically to mouse skin once a day for four consecutive days and adduct levels were determined in DNA from skin and lung by (32)P-post-labelling analysis. Relatively higher levels of bulky aromatic DNA adducts were noted in mouse skin treated with MS from a single Indian non-filter (INF) cigarette when compared with MS of a single bidi (with about half the product weight and one-quarter the tobacco compared with a cigarette), while comparable adduct levels were noted with SS from these two products. Considering the differences in the yields of constituents of tobacco smoke from the different products analyzed, the genotoxic potential of INF, Indian filter king (IFK) and American filter (AF) cigarettes as well as bidis was determined by topically applying an equal amount of TPM (rather than equal product-derived TPM). SS-derived TPM from all the products showed relatively higher levels of total polycyclic aromatic hydrocarbons and induced relatively higher levels of bulky aromatic DNA adducts than those derived from MS. The data indicate that TPM (MS + SS) from cigarettes appears to be more genotoxic than that from bidis and the contribution of tendu leaf (a non-tobacco bidi wrapper) to the generation of bulky aromatic DNA adducts appears to be significant, particularly in SS of bidis. Topical pretreatment with curcumin decreased the levels of TPM-derived adducts while pretreatment with dietary turmeric failed to show such protection.
authors.affiliation: Tobacco Carcinogenesis Group, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai-410 208, India.
authors.forename: Rachana
authors.initials: R
authors.lastname: Thapliyal
authors.forename: Shashadhar S
authors.initials: SS
authors.lastname: Dolas
authors.forename: Shrirang S
authors.initials: SS
authors.lastname: Pakhale
authors.forename: Girish B
authors.initials: GB
authors.lastname: Maru
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000818:Animals; D030024:Curcuma / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology; D018736:DNA Adducts; D004249:DNA Damage; D008168:Lung / Q000187:drug effects*; D008297:Male; D051379:Mice; D008836:Micrococcal Nuclease / Q000494:pharmacology; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000494:pharmacology; D012867:Skin / Q000187:drug effects; D012906:Smoke; D012907:Smoking / Q000009:adverse effects*; D013997:Time Factors; D014026:Nicotiana
chemical_list: D018736:DNA Adducts; D010936:Plant Extracts; D012906:Smoke; D000096:Acetone; D008836:Micrococcal Nuclease; D003474:Curcumin