title: Glutamine metabolism in Crohn's disease: a stable isotope study.
abstract: OBJECTIVE
  To determine whether chronic intestinal inflammation alters glutamine utilization, six 31 +/- 6 yr-old patients with Crohn's disease and an age-matched group of 6 healthy subjects received 7-h intravenous infusions of L-[5,5,5-2H3]leucine, along with an infusion of L-[1-13C]glutamine delivered intravenously for the first 3.5 h, and via a nasogastric tube for the subsequent 3.5 hrs. None of the patients was receiving any nutritional supplement or antiinflammatory drug. All were in remission (Crohn's disease activity index < 150) and in a near-normal nutritional status.
  
  
  METHODS
  We used plasma 2H3-alpha-ketoisocaproate to determine leucine appearance rate (Ra), and plasma 13C-glutamine and breath 13CO2 to determine glutamine Ra and oxidation, respectively. The fraction of enteral glutamine undergoing uptake in the splanchnic bed was determined from the difference in plasma 13C-glutamine enrichments between the intravenous and nasogastric 13C-glutamine infusion periods.
  
  
  RESULTS
  Neither leucine Ra, nor plasma glutamine concentration (526 +/- 40 vs. 530 +/- 50 micromol/l), glutamine Ra (364 +/- 19 vs. 355 +/- 24 micromol kg(-1) h(-1)), or splanchnic glutamine uptake (61 +/- 5 vs. 65 +/- 2%) differed between groups. In both groups, glutamine oxidation rose when the glutamine tracer was supplied enterally, compared with the intravenous route (70 +/- 6 vs. 39 +/- 2% in patients; 69 +/- 2 vs. 38 +/- 1% in controls), but did not differ between groups.
  
  
  CONCLUSIONS
  When in remission, patients with Crohn's disease have normal rates of proteolysis, and glutamine production, utilization, oxidation, and splanchnic uptake. The data suggest there is no obvious requirement for glutamine in patients with quiescent Crohn's disease.
authors.affiliation: INSERM U.539, Human Nutrition Research Center, Hôtel-Dieu Hospital, 2ème étage aile nord, 44093 Nantes Cedex 1, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bourreille
authors.forename: Bernard
authors.initials: B
authors.lastname: Humbert
authors.forename: Pascale
authors.initials: P
authors.lastname: Maugère
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Galmiche
authors.forename: Dominique
authors.initials: D
authors.lastname: Darmaun
mesh_terms: D000328:Adult; D002153:Calorimetry, Indirect; D002247:Carbon Isotopes; D016022:Case-Control Studies; D003424:Crohn Disease / Q000378:metabolism*; D003903:Deuterium; D005260:Female; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D007262:Infusions, Intravenous; D007441:Intubation, Gastrointestinal; D007930:Leucine / Q000378:metabolism; D008297:Male; D009751:Nutritional Requirements; D010101:Oxygen Consumption; D013152:Splanchnic Circulation / Q000502:physiology
chemical_list: D002247:Carbon Isotopes; D005973:Glutamine; D003903:Deuterium; D007930:Leucine