Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.).
Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO(3) under a 16-h photoperiod. After 3-4 weeks of culture, 21.9-80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2-3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO(3) were diploid.
Han
J-S
JS
National Horticultural Research Institute, Rural Development Administration, Suwon 441-440, South Korea.
Oh
D-G
DG
Mok
I-G
IG
Park
H-G
HG
Kim
C K
CK
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000613:Aminobutyrates / Q000494:pharmacology; D001593:Benzyl Compounds; D018548:Cotyledon / Q000254:growth & development*; D019662:Cucurbitaceae / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D005434:Flow Cytometry; D005838:Genotype; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development*; D011003:Ploidies; D011687:Purines; D012835:Silver Nitrate / Q000494:pharmacology
D000613:Aminobutyrates; D001593:Benzyl Compounds; D003470:Culture Media; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C034182:1-naphthaleneacetic acid; C012175:2-amino-4-(2-aminoethoxy)butanoic acid; C030737:indoleacetic acid; D012835:Silver Nitrate; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin