A variant of porcine thyroxine-binding globulin has reduced affinity for thyroxine and is associated with testis size.
The field of genomics applies the dissection of genetic differences toward an understanding of the biology of complex traits. Quantitative trait loci (QTL) for testis size, plasma FSH in boars, and body composition (backfat) have been identified near the centromere on the X chromosome in a Meishan-White Composite resource population. Since thyroid function affects Sertoli cell development and adult testis size in rodents, and thyroxine-binding globulin (TBG) maps to this region on the porcine X chromosome, TBG was a positional candidate gene for testis size. We discovered a polymorphism in exon 2 of the porcine TBG gene that results in an amino acid change of the consensus histidine to an asparagine. This single nucleotide polymorphism (SNP) resides in the ligand-binding domain of the mature polypeptide, and the Meishan allele is the conserved allele found in human, bovine, sheep, and rodent TBG. Binding studies indicate altered binding characteristics of the allelic variants of TBG with the asparagine (White Composite) isoform having significantly greater affinity for thyroxine than the histidine (Meishan) isoform. Alternate alleles in boars from the resource population are also significantly associated with testis weight. Therefore, this polymorphism in TBG is a candidate for the causative variation affecting testis size in boars.
Nonneman
Dan
D
USDA-ARS, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA. nonneman@email.marc.usda.gov
Rohrer
Gary A
GA
Wise
Tommy H
TH
Lunstra
Donald D
DD
Ford
J Joe
JJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005640:Follicle Stimulating Hormone / Q000097:blood; D014644:Genetic Variation; D006358:Hot Temperature; D008297:Male; D008969:Molecular Sequence Data; D009929:Organ Size / Q000235:genetics; D020161:Physical Chromosome Mapping; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017421:Sequence Analysis; D012708:Sertoli Cells / Q000166:cytology / Q000502:physiology; D013552:Swine / Q000235:genetics*; D013737:Testis / Q000033:anatomy & histology / Q000502:physiology*; D013963:Thyroid Hormones / Q000097:blood; D013974:Thyroxine / Q000378:metabolism*; D013975:Thyroxine-Binding Proteins / Q000235:genetics* / Q000378:metabolism*
D013963:Thyroid Hormones; D013975:Thyroxine-Binding Proteins; D005640:Follicle Stimulating Hormone; D013974:Thyroxine