beta-globin DNA in maternal plasma as a molecular marker of pre-eclampsia.
OBJECTIVE
Levels of cell-free foetal DNA in maternal plasma are higher in the presence of clinical features of pre-eclampsia (PE). However, currently, this method is informative only in women bearing a male foetus, by amplification of Y-specific sequences. In the present study, we overcame this limitation by examining quantitative distribution of beta-globin, a foetal gender-independent DNA marker.


METHODS
We quantified beta-globin concentrations in the plasma of 207 pregnant women: control group, 164 subjects; affected group, 43 women affected by PE (n = 43). beta-globin concentrations were converted into multiples of the median of the controls (MoM), in order to assess the possible different distribution of beta-globin MoM in cases and controls.


RESULTS
Adjusted MoM values were as follows: controls, 1.00 +/- 0.71; affected group 4.03 +/- 3.77 (p-value < 0.001). Among the PE affected cases, MoM beta-globin values of cases with foetal growth restriction (FGR) were almost twice as great as those cases without FGR (p-value = 0.003).


CONCLUSIONS
beta-globin levels are higher in the plasma of pregnant women with PE, especially in those cases complicated with FGR, and do not depend on foetal gender. Such a molecular marker can potentially be used in evaluating the pathophysiological severity of PE.
Sekizawa
Akihiko
A
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan. sekizawa@med.showa-u.ac.jp
Farina
Antonio
A
Koide
Keiko
K
Iwasaki
Mariko
M
Honma
Susumi
S
Ichizuka
Kiyotake
K
Saito
Hiroshi
H
Okai
Takashi
T
D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004247:DNA / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005317:Fetal Growth Retardation / Q000097:blood; D005914:Globins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008431:Maternal-Fetal Exchange; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
D015415:Biomarkers; D005914:Globins; D004247:DNA