title: Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
abstract: We have been conducting a mouse cDNA project to predict protein-coding sequences of mouse homologues of human KIAA and FLJ genes since 2001. As an extension of these projects, we herein present the entire sequences of 500 mKIAA cDNA clones and 4 novel cDNA clones that were incidentally identified during this project. We have isolated cDNA clones from the size-fractionated mouse cDNA libraries derived from 7 tissues and 3 types of cultured cells. The average size of the 504 cDNA sequences reached 4.3 kb and that of the deduced amino acid sequences from these cDNAs was 807 amino acid residues. We assigned the integrity of CDSs from the comparison with the corresponding human KIAA cDNA sequences. The comparison of mouse and human sequences revealed that two different human KIAA cDNAs are derived from single genes. Furthermore, 3 out of 4 proteins encoded in the novel cDNA clones showed moderate sequence similarity with human KIAA proteins, thus we could obtain new members of KIAA protein families through our mouse cDNA projects.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Okazaki
authors.forename: Reiko
authors.initials: R
authors.lastname: F-Kikuno
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohara
authors.forename: Susumu
authors.initials: S
authors.lastname: Inamoto
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Koseki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hiraoka
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
authors.forename: Susumu
authors.initials: S
authors.lastname: Seino
authors.forename: Motoi
authors.initials: M
authors.lastname: Nishimura
authors.forename: Tsuneyasu
authors.initials: T
authors.lastname: Kaisho
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Hoshino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagase
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koga
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D018076:DNA, Complementary; D030541:Databases, Genetic; D006801:Humans; D051379:Mice; D005810:Multigene Family; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D011506:Proteins