A radiation hybrid map of chicken Chromosome 4.
The mapping resolution of the physical map for chicken Chromosome 4 (GGA4) was improved by a combination of radiation hybrid (RH) mapping and bacterial artificial chromosome (BAC) mapping. The ChickRH6 hybrid panel was used to construct an RH map of GGA4. Eleven microsatellites known to be located on GGA4 were included as anchors to the genetic linkage map for this chromosome. Based on the known conserved synteny between GGA4 and human Chromosomes 4 and X, sequences were identified for the orthologous chicken genes from these human chromosomes by BLAST analysis. These sequences were subsequently used for the development of STS markers to be typed on the RH panel. Using a logarithm of the odds (LOD) threshold of 5.0, nine linkage groups could be constructed which were aligned with the genetic linkage map of this chromosome. The resulting RH map consisted of the 11 microsatellite markers and 50 genes. To further increase the number of genes on the map and to provide additional anchor points for the physical BAC map of this chromosome, BAC clones were identified for 22 microsatellites and 99 genes. The combined RH and BAC mapping approach resulted in the mapping of 61 genes on GGA4 increasing the resolution of the chicken-human comparative map for this chromosome. This enhanced comparative mapping resolution enabled the identification of multiple rearrangements between GGA4 and human Chromosomes 4q and Xp.
Rabie
Tarik S K M
TS
Wageningen Institute of Animal Sciences, Animal Breeding and Genetics Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands. Tarik@egypt.net
Crooijmans
Richard P M A
RP
Morisson
Mireille
M
Andryszkiewicz
Joanna
J
van der Poel
Jan J
JJ
Vignal
Alain
A
Groenen
Martien A M
MA
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D019992:Databases as Topic; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D008126:Lod Score; D018895:Microsatellite Repeats; D008957:Models, Genetic; D021101:Radiation Hybrid Mapping / Q000379:methods*; D012984:Software