Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment.
OBJECTIVE
To estimate ischemic tissue damage in ovarian cortex and to evaluate the effectiveness of ascorbic acid, an antioxidant, to protect ovarian tissue from apoptosis caused by ischemia.


METHODS
In vitro laboratory experiments.


METHODS
Academic research institute.


METHODS
Fresh and frozen/thawed cortical sections of bovine ovaries were incubated in MEM medium with or without ascorbic acid for a duration of 3, 24, and 48 hours at 37 degrees C.


METHODS
Oxygen consumption rates, lactate dehydrogenase concentrations, apoptosis rates determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and DNA fragmentation analysis.


RESULTS
The oxygen consumption rates were correlated inversely with the duration of incubation. When the rates of apoptosis in primordial follicles with or without ascorbic acid treatment were compared, there was no statistically significant difference between the two groups. However, the ascorbic acid treatment group showed significantly decreased apoptosis in ovarian cortex (stromal cells) with 24 hours of incubation.


CONCLUSIONS
The correlation between ischemic tissue damage and the duration of ischemia was verified. Ovarian cortex could tolerate ischemia at least for 3 hours. Ascorbic acid treatment reduced apoptosis in ovarian cortex up to 24 hours of incubation in vitro. It appeared that stromal cells were more vulnerable to ischemia compared to primordial follicles.
Kim
S Samuel
SS
Eulji Life Science Institute, Eulji University School of Medicine, Seoul, South Korea. medssk@attglobal.net
Yang
Hyun Won
HW
Kang
Hee Gyoo
HG
Lee
Hang Heun
HH
Lee
Hoi Chang
HC
Ko
Duck Sung
DS
Gosden
Roger G
RG
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000473:pathology*; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption
D000975:Antioxidants; D001205:Ascorbic Acid