title: Simultaneous determination of vitamins C, E and beta-carotene in human plasma by high-performance liquid chromatography with photodiode-array detection.
abstract: OBJECTIVE
  To develop a high-performance liquid chromatography (HPLC) method with photodiode-array ultraviolet detection for the simultaneous determination of vitamin C, vitamin E and beta-carotene.
  
  
  METHODS
  Following liquid-phase extraction from the human plasma samples, these three vitamins were successfully separated on the LiChrospher 100 RP-18 column (125 x 4 mm I.D.; particle size, 5 microm) at a flow-rate of 1.2 ml/min, with a mobile phase of methanol-acetonitrile-tetrahydrofuran (75: 20: 5, v/v/v).
  
  
  RESULTS
  The limits of quantitation were 100, 0.25 and 0.25 microg/ml for vitamin C, vitamin E and beta-carotene respectively. The method is linear over the studied range of 0.25 to 5 g/ml for vitamin E and beta-carotene and 100 to 5000 microg/ml for vitamin C. The extraction recoveries were greater than 83% for these three vitamins. The within day and between-day precision of the analysis did not exceed 15.3 and 16.2%, respectively.
  
  
  CONCLUSIONS
  A suitable method to determine the concentration of vitamin C, vitamin E and beta-carotene following oral administration of antioxidant supplement capsules to a healthy Chinese volunteer.
authors.affiliation: Defence Medical and Environmental Research Institute, DSO National Laboratories, (DMERI @ DSO), Singapore, Republic of Singapore.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Su-Yin
authors.initials: SY
authors.lastname: Tham
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.forename: Mui Hoon
authors.initials: MH
authors.lastname: Lai
authors.forename: Lionel K H
authors.initials: LK
authors.lastname: Lee
authors.forename: Shabbir M
authors.initials: SM
authors.lastname: Moochhala
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008297:Male; D010777:Photochemistry / Q000295:instrumentation; D012044:Regression Analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000097:blood*; D019207:beta Carotene / Q000097:blood*
chemical_list: D000975:Antioxidants; D019207:beta Carotene; D014810:Vitamin E; D001205:Ascorbic Acid