Novel fabricated matrix via electrospinning for tissue engineering.
Electric field-driven fiber formation (electrospinning) is developing into a practical means for preparing novel porous filament with unusual structures and affordable mechanical properties. Polycaprolactone (PCL) was dissolved in solvent mixtures of methylene chloride/N,N-dimethyl formamide with ratios of 100/0, 75/25, and 50/50 (v/v) for electrospinning. The filament was formed by coagulation of the spinning solution following the well-known principle of phase separation in polymer solutions valid in other wet shaping processes. A strand of electrospun porous filament consisted of fibers ranging from 0.5 to 12 microm in diameter. To evaluate the feasibility of three-dimensional fabric as scaffold matrices, the plain weave, which is the simplest of the weaves and the most common, was prepared with porous PCL filament. The growth characteristics of MCF-7 mammary carcinoma cells in the woven fabrics showed the important role of matrix microstructure in proliferation. This study has shown that woven fabrics, consisting of porous filaments via electrospinning, may be suitable candidates as tissue engineering scaffolds.
Khil
Myung-Seob
MS
Department of Textile Engineering, Chonbuk National University, Chonju, 561-756 South Korea.
Bhattarai
Shanta Raj
SR
Kim
Hak-Yong
HY
Kim
Sung-Zoo
SZ
Lee
Keun-Hyung
KH
D002277:Carcinoma / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D003460:Crystallization; D004126:Dimethylformamide; D004583:Electrons; D006801:Humans; D007700:Kinetics; D019563:Mechanics; D008752:Methylene Chloride; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D012996:Solutions; D013500:Surface Tension; D023822:Tissue Engineering / Q000295:instrumentation*; D014783:Viscosity; D014961:X-Ray Diffraction
D011091:Polyesters; D012996:Solutions; C016240:polycaprolactone; D008752:Methylene Chloride; D004126:Dimethylformamide