title: Presence and release of the chromogranin B-derived secretolytin-like peptide KR-11 from the porcine spleen.
abstract: Chromogranin B (CgB) is a major matrix protein in secretory granules/large dense-cored vesicles and a precursor for smaller peptides. In an earlier study, we have identified a secretolytin-like peptide (KR-11, pCgB(637-647)) from porcine chromaffin granules. Further evidence is presented here to show the processing of chromogranin B to this peptide during axonal transport in the splenic nerve and its release in the spleen upon various conditions of stimulation. Immunohistochemical staining showed that in the porcine spleen chromogranin B and NPY completely colocalize in nerve fibres and varicosities in blood vessel walls and trabeculae, and along the loose network of smooth muscle cells in the red pulp, as identified by their alpha-smooth muscle cell actin content. No antibacterial activity was found for the porcine secretolytin-like peptide, KR-11. The change of one amino acid residue (Thr-->Asn) in the porcine homologous fragment of secretolytin appears to be responsible for its loss of antibacterial activity.
authors.affiliation: Laboratory of Neurobiology and Neuropharmacology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Depreitere
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Liang
authors.forename: Edmond
authors.initials: E
authors.lastname: Coen
authors.forename: Etienne J
authors.initials: EJ
authors.lastname: Nouwen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001369:Axons / Q000378:metabolism; D001692:Biological Transport; D002864:Chromogranins / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004558:Electric Stimulation; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010477:Perfusion; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D002864:Chromogranins; D009478:Neuropeptide Y; D010446:Peptide Fragments