[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].
OBJECTIVE
To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.


METHODS
Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.


RESULTS
The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.


CONCLUSIONS
The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.
Wang
Yong-ning
YN
Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing 100101, China.
Li
Rong-xiu
RX
Li
Cheng-wu
CW
Gao
Yun-hua
YH
Guo
Xiao-ying
XY
Gu
Zhong-ze
ZZ
D000218:Adamantane / Q000031:analogs & derivatives; D006801:Humans; D006961:Hyperparathyroidism / Q000097:blood; D007011:Hypoparathyroidism / Q000097:blood; D007118:Immunoassay / Q000379:methods; D049449:Luminescence; D013972:Thyrotropin / Q000097:blood*
C071872:3-(2'-(spiro-5-chloroadamantane))-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; D013972:Thyrotropin; D000218:Adamantane