title: Comparative study of biochemical methods for the identification of Dichelobacter nodosus.
abstract: The aim of this study was to compare four identification procedures to detect Dichelobacter nodosus and develop a rapid, simple and effective method to identify D. nodosus strains isolated from cases of ovine footrot. The four methods used were: (a) the classic guidelines set down by Holdeman et al. (1977) and Summanen et al. (1993) which are based on gas liquid chromatography (GLC) and different biochemical tests, this method was considered as landmark; (b) Baron and Citron's flowchart for the rapid identification of Gram-negative rod-shaped anaerobes (1997); (c) the API rapid 32 A system (bio Mérieux), and (d) Mast ID Anaerobe ID Ring (MID8) (Mast Diagnostics). None of the four methods used allowed us to correctly identify the D. nodosus strains (neither the strains isolated from cases of ovine footrot nor those originating from type collection). Because of the difficulties encountered in obtaining a correct identification of D. nodosus, we propose a simple, rapid and effective way to achieve this task. Our flowchart will provide the means to identify this microorganism in any laboratory of general microbiology without having to use any specialised equipment.
authors.affiliation: Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s/n, 10071 Cáceres, Spain.
authors.forename: Ruth
authors.initials: R
authors.lastname: Jiménez
authors.forename: S
authors.initials: S
authors.lastname: Píriz
authors.forename: E M
authors.initials: EM
authors.lastname: Mateos
authors.forename: S
authors.initials: S
authors.lastname: Vadillo
mesh_terms: D000818:Animals; D020376:Dichelobacter nodosus / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D005535:Foot Rot / Q000175:diagnosis / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology*; D012985:Software Design; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors