[Clinical application of the activated partial thromboplastin time (APTT) with modified incubation time for the diagnosis of lupus anticoagulant (LA)].
BACKGROUND
Although several tests are used to screen for the presence of LA, none detects all its types. The shortening of APT observed when the pre-incubation period is prolonged, proved to be a sensitive test for the presence of LA.


METHODS
We determined the APTT, performed with a 4 or 15 min preincubation period (APETs and APTT15 respectively), in 22 healthy subjects, 3 commercial positive controls for LA, 16 patients with a previous diagnosis of LA and 54 patients with recurrent fetal loss and/or infertility. Evidence of LA was established by a positive Staclot-LA test.


RESULTS
APTTs and APTT15 were 31.5 +/- 4.7 and 28.4 +/- 4.5 seconds respectively in samples from 22 normal subjects. The figures in samples with LA, were 71.5 +/- 20.3 s and 58.6 +/- 18 s respectively. The difference between the two APTTs performed on an individual sample was defined as the APTT 4-15 and was 2.6 +/- 2.0 in normal subjects 2.5 +/- 2.8 in 13 patients anticoagulated with warfarin, -10.0 +/- 6.5 in 13 patients receiving heparin, and 13.2 +/- 4.9 in 15 patients with LA. The test values for LA patients were significantly higher than those for normal subjects (p < 0.0001). For values over 5, the APTT 4-15 had 93.3% sensitivity and 100% specificity. In one patient with recurrent fetal loss or infertility, who was LA positive, the APTT 4-15 was positive with a value of 14.


CONCLUSIONS
This modified TTPA is easy to perform, and provides a reasonably discriminatory value for the presence of LA. Therefore, we recommend the TTPA 4-15 to screen for LA.
Fardella
Patricia
P
Servicio de Hematología, Hospital Clinico Universidad de Chile, Santiago, Chile. pfardella123@msn.com
Rustagi
Pradip K
PK
D000328:Adult; D000925:Anticoagulants / Q000627:therapeutic use*; D016736:Antiphospholipid Syndrome / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology; D016022:Case-Control Studies; D005189:False Positive Reactions; D005260:Female; D006493:Heparin / Q000627:therapeutic use*; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000097:blood*; D010314:Partial Thromboplastin Time; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis*; D011517:Prothrombin Time; D012680:Sensitivity and Specificity; D013918:Thrombin Time; D014859:Warfarin / Q000627:therapeutic use
D000925:Anticoagulants; D016682:Lupus Coagulation Inhibitor; D014859:Warfarin; D006493:Heparin
Aplicación del tiempo de tromboplastina parcial activado (TTPA) con tiempo de incubación modificado en el diagnóstico del anticoagulante lúpico (AL).