title: Mycobacterium canariasense sp. nov.
abstract: A novel rapidly growing, non-pigmented mycobacterium was isolated from blood samples obtained from 17 patients with febrile syndrome. Bacterial growth occurred at 30 and 37 degrees C on Löwenstein-Jensen medium and also on MacConkey agar without crystal violet. Strains contained alpha- and alpha'-mycolates in their cell wall. Sequence analysis of the hsp65 and 16S rRNA genes identified the isolates as rapidly growing mycobacteria. Sequences of both genes were unique within the mycobacteria. DNA-DNA hybridization showed that the isolates had less than 15 % reassociation with 13 other recognized rapidly growing mycobacteria. The name Mycobacterium canariasense sp. nov. is proposed for this novel opportunistic pathogen, which is most closely related to Mycobacterium diernhoferi. The type strain is 502329T (= CIP 107998T = CCUG 47953T).
authors.affiliation: Laboratorio de Micobacterias, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: M Soledad
authors.initials: MS
authors.lastname: Jiménez
authors.affiliation: Servicio de Microbiología, Hospital de Gran Canaria Dr Negrin, Las Palmas de Gran Canaria, Spain.
authors.forename: M Isolina
authors.initials: MI
authors.lastname: Campos-Herrero
authors.affiliation: Servicio de Microbiología, Hospital de Gran Canaria Dr Negrin, Las Palmas de Gran Canaria, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: García
authors.affiliation: Unitat de Microbiología, Departament de Genetica i de Microbiología, Universitat Autónoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Luquin
authors.affiliation: Laboratorio de Micobacterias, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Herrera
authors.affiliation: Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: García
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D002473:Cell Wall / Q000737:chemistry; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D003470:Culture Media; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D009164:Mycobacterium Infections / Q000382:microbiology; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009171:Mycolic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins