[Establishment and biological characteristics of orthotopic transplantation tumor model of hepatocellular carcinoma in mice].
OBJECTIVE
To study the feasibility of the establishment of the orthotopic transplantation tumor model of hepatocellular carcinoma in mice and its tumor biological characteristics.


METHODS
H22 cells of hepatocellular carcinoma were inoculated to form ectopic transplanted model in mice by subcutaneous injection. Then the subcutaneous tumors were implanted into the liver of mice, and the orthotopic transplantation tumor model of hepatocellular carcinoma was established.


RESULTS
The successful rate of the orthotopic transplantation tumor model was 95.6% and the spontaneous metastatic rate was 81.8%, the rate of mass ascites was 40.9% and the natural extinctive rate was 0%. The natural survival time in the orthotopic transplantation tumor model was 28 days and the proliferation of tumor in transplanted model was accelerated after 2 weeks or so.


CONCLUSIONS
The orthotopic transplantation tumor model in mice is an ideal model for studying the metastatic mechanism and screening anti-tumor drugs for liver cancer, just because of its high successful rate and high spontaneous metastatic rate with no natural extinction.
Han
Ke-Qi
KQ
Department of Tranditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. keqihan@yahoo.com
Gu
Wei
W
Hu
Xia
X
Zhang
Ya-Ni
YN
Huang
Xue-Qiang
XQ
Liu
Ling
L
Su
Yong-Hua
YH
Ling
Chang-Quan
CQ
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation / Q000379:methods