Spatial analysis of spike-timing-dependent LTP and LTD in the CA1 area of hippocampal slices using optical imaging.
Spike-timing-dependent long-term potentiation (LTP) and long-term depression (LTD) were investigated in the CA1 area of hippocampal slices using optical imaging. A pair of electrical pulses were used to stimulate the Schaffer-commissural collateral and the stratum oriens with various sets of relative timing (tau) between the two stimuli. These sets of paired pulses gave rise to LTP/LTD, whose induction was closely related to tau, and their profiles were classified into two types depending on their layer specific location along the dendrite. One was characterized by a symmetric time window observed in the proximal region of the stratum radiatum (SR) and the other by an asymmetric time window in the distal region of the SR. Bath application of bicuculline (gamma-aminobutyric acid [GABA] receptor antagonist) to hippocampal slices revealed that GABAergic interneuron projections were responsible for the symmetry of a time window.
Tsukada
Minoru
M
Department of Information and Communication Engineering, Faculty of Engineering, Tamagawa University, Tokyo, Japan. tsukada@eng.tamagawa.ac.jp
Aihara
Takeshi
T
Kobayashi
Yuki
Y
Shimazaki
Hideaki
H
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D003712:Dendrites / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D004594:Electrophysiology / Q000379:methods; D005260:Female; D018756:GABA Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D007395:Interneurons / Q000187:drug effects / Q000378:metabolism; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D036881:Long-Term Synaptic Depression / Q000187:drug effects / Q000502:physiology*; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009482:Neurophysiology / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018756:GABA Antagonists; D005680:gamma-Aminobutyric Acid