title: Metabolic profile of the hippocampus of Zucker Diabetic Fatty rats assessed by in vivo 1H magnetic resonance spectroscopy.
abstract: Localized in vivo 1H magnetic resonance spectroscopy (MRS) was used to investigate metabolite levels in the brain of adult Zucker Diabetic Fatty (ZDF) rats, an animal model for type 2 diabetes mellitus. This study focussed on the hippocampus, assumed to be one of the main brain areas affected by this disease. Together with an almost 5-fold increase in blood glucose concentration measured by glucose oxidation, significant increases were found in the hippocampal concentrations of glucose (4.93 vs 1.66 mM p < 0.001), myo-inositol (6.52 vs 4.30 mM; p < 0.05), and total creatine (12.71 vs 10.50 mM; p < 0.05) in ZDF rats (n = 5) compared with littermates (n = 5). Although no obvious alterations were detected in the hippocampal levels of other metabolites, including NAA + NAAG and choline-containing compounds in the ZDF rats, the increase in Glc and Ins levels is in line with elevated brain tissue contents of these metabolites in patients with diabetes mellitus.
authors.affiliation: Department of Radiology, University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Marinette
authors.initials: M
authors.lastname: van der Graaf
authors.forename: Susan W J
authors.initials: SW
authors.lastname: Janssen
authors.forename: Jack J A
authors.initials: JJ
authors.lastname: van Asten
authors.forename: Ad R M M
authors.initials: AR
authors.lastname: Hermus
authors.forename: C G J
authors.initials: CG
authors.lastname: Sweep
authors.forename: Jeroen A
authors.initials: JA
authors.lastname: Pikkemaat
authors.forename: Gerard J M
authors.initials: GJ
authors.lastname: Martens
authors.forename: Arend
authors.initials: A
authors.lastname: Heerschap
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011522:Protons; D051381:Rats; D011924:Rats, Zucker
chemical_list: D001786:Blood Glucose; D011522:Protons