LC/MS/MS identification of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregates.
Cinnamyl alcohol was added to the media of compact callus aggregates (CCA) of Rhodiola rosea for stimulating the production of cinnamyl glycosides. The biotransformation reaction produced high amounts of rosin, while only a very low amount of rosavin was produced. As the consumption rate of cinnamyl alcohol was much higher than production of rosin, the aqueous methanol extracts of compact callus aggregates were studied by liquid chromatography-mass spectrometric methods and four new unexpected biotransformation products of cinnamyl alcohol were identified.
Tolonen
Ari
A
Department of Chemistry, University of Oulu, PO Box 3000, 90014 Oulu, Finland. ari.tolonen@oulu.fi
György
Zsuzsanna
Z
Jalonen
Jorma
J
Neubauer
Peter
P
Hohtola
Anja
A
D001711:Biotransformation; D002853:Chromatography, Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis* / Q000096:biosynthesis*; D013058:Mass Spectrometry / Q000379:methods*; D020005:Propanols / Q000378:metabolism*; D012116:Resins, Plant / Q000032:analysis / Q000378:metabolism; D029771:Rhodiola / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D006027:Glycosides; D020005:Propanols; D012116:Resins, Plant; C013893:rosin; C020722:cinnamyl alcohol