title: Functional analysis of the homoserine O-acetyltransferase gene and its identification as a selectable marker in Gibberella zeae.
abstract: We used restriction enzyme-mediated integration (REMI) to identify a methionine auxotrophic mutant of Gibberella zeae, an important cereal pathogen. In addition to its methionine requirement, the G. zeae REMI mutant designated Z43R3912 showed pleiotropic phenotypes, including reduced virulence on host plants and lack of sexual development. Outcrossing of Z43R3912 with a mat1-1 deletion strain confirmed that the mutation of Z43R3912 was tagged with the hygromycin B resistance marker. The vector insertion site in Z43R3912 was identified within the ORF designated GzmetE, encoding a putative homoserine O-acetyltrasferase (HOA). Gene disruption analyses confirmed that GzmetE was responsible for the pleiotropic phenotypes of Z43R3912. Genetic complementation of the G. zeae methionine auxotroph with an intact copy of the Aspergillus nidulans metE and GzmetE genes suggests that the HOA gene can be used as a selectable marker for transformation of G. zeae.
authors.affiliation: School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Korea.
authors.forename: You-Kyoung
authors.initials: YK
authors.lastname: Han
authors.forename: Theresa
authors.initials: T
authors.lastname: Lee
authors.forename: Kap-Hoon
authors.initials: KH
authors.lastname: Han
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Yun
authors.forename: Yin-Won
authors.initials: YW
authors.lastname: Lee
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D005819:Genetic Markers; D005874:Gibberella / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D001467:Hordeum / Q000382:microbiology; D010641:Phenotype; D003313:Zea mays / Q000382:microbiology
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D000123:Acetyltransferases; C021369:homoserine O-acetyltransferase