title: Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness.
abstract: The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns.
authors.affiliation: Department of Proteomics, Molecular Probes, Eugene, Oregon, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ge
authors.forename: Lakshmanswamy
authors.initials: L
authors.lastname: Rajkumar
authors.forename: Raphael C
authors.initials: RC
authors.lastname: Guzman
authors.forename: Satyabrata
authors.initials: S
authors.lastname: Nandi
authors.forename: Wayne F
authors.initials: WF
authors.lastname: Patton
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Agnew
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006031:Glycosylation; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D051381:Rats
chemical_list: D010750:Phosphoproteins; D011506:Proteins