title: A new C-type animal lectin isolated from Bothrops pirajai is responsible for the snake venom major effects in the isolated kidney.
abstract: We investigated the biochemical and biological effects of a new C-type galactoside specific lectin termed BPL that was isolated from the snake venom of Bothrops pirajai. This lectin was purified using size exclusion HPLC followed by an immobilized lactose affinity column. The purified BPL was homogeneous by reverse phase HPLC and SDS-PAGE. We evaluated the nephrotoxicity of the whole venom of B. pirajai and its lectin. The whole venom of B. pirajai (10 microg/mL) showed similar results as those observed for BPL (3, 10 and 30 microg/mL) evaluated by the perfused rat kidney method. They caused reductions in perfusion pressure (Control120 = 110.28 +/- 3.69; BP120 = 70.70 +/- 2.40*; BPL3(120) = 113.20 +/- 4.40; BPL10(120) = 67.80 +/- 3.00*; BPL30(120) = 64.90 +/- 3.50* mmHg; *: P < 0.05), renal vascular resistance, urinary flow, glomerular filtration rate (Control90 = 0.695 +/- 0.074; BP90 = 0.142 +/- 0.032*; BPL3(90) = 0.314 +/- 0.064; BPL10(90) = 0.250 +/- 0.038*; BPL30(90) = 0.088 +/- 0.021* mLg(-1) min(-1); *: P < 0.05) and sodium (Control120 = 81.28 +/- 0.26; BP120 = 55.71 +/- 5.72*; BPL3(120) = 80.94 +/- 0.93; BPL10(120) = 65.23 +/- 1.47*; BPL30(120) = 76.03 +/- 1.70* %; *: P < 0.05), potassium and chloride tubular transport. Neither whole venom nor purified BPL induced direct vasoactive effects in perfused arteriolar mesenteric bed, and BPL did not potentiate bradykinin contraction in the ileum. We postulate that both B. pirajai and BPL promoted the same renal effects probably caused by the release of inflammatory mediators.
authors.affiliation: Departament of Physiology and Pharmacology, Federal University of Ceará, Coronel Nunes de Melo1127, CEP 60430-270 Fortaleza, Ceará, Brazil. ahavt@secrel.com.br
authors.forename: Alexandre
authors.initials: A
authors.lastname: Havt
authors.forename: Marcos Hikari
authors.initials: MH
authors.lastname: Toyama
authors.forename: Nilberto Robson Falcão
authors.initials: NR
authors.lastname: do Nascimento
authors.forename: Daniela Oliveira
authors.initials: DO
authors.lastname: Toyama
authors.forename: Arlandia Cristina Lima
authors.initials: AC
authors.lastname: Nobre
authors.forename: Alice Maria Costa
authors.initials: AM
authors.lastname: Martins
authors.forename: Paulo Sérgio Ferreira
authors.initials: PS
authors.lastname: Barbosa
authors.forename: Jose Camillo
authors.initials: JC
authors.lastname: Novello
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Boschero
authors.forename: Everardo M
authors.initials: EM
authors.lastname: Carneiro
authors.forename: Manassés Claudino
authors.initials: MC
authors.lastname: Fonteles
authors.forename: Helena Serra Azul
authors.initials: HS
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D017837:Bothrops; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry* / Q000633:toxicity*; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology*; D037181:Lectins, C-Type / Q000302:isolation & purification; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014655:Vascular Resistance / Q000187:drug effects*; D014664:Vasodilation / Q000187:drug effects
chemical_list: D003435:Crotalid Venoms; D018836:Inflammation Mediators; D037181:Lectins, C-Type