Synthesis and characterization of a photocleavable cross-linker and its application on tunable surface modification and protein photodelivery.
A heterobifunctional photocleavable cross-linker based on an o-nitrobenzyl ester moiety was synthesized. The cross-linker has N-hydroxysuccinimidyl and disulfide groups attached at each end and thus can anchor a protein to a gold-coated substrate surface. Steady-state spectroscopic studies suggest that the cross-linker undergoes a clean C-O fragmentation upon irradiation with a quantum yield of 0.1. Consequently, immobilized proteins (such as avidin or antibodies) on a substrate surface can be released efficiently (>95%) under UV irradiation (lambda > 300 nm) without degrading the protein functionality. We also demonstrated protein delivery via bioconjugation of protein molecules to a gold-coated atomic-force microscope (AFM) tip. When the proteins are photoreleased from the AFM tip, they are delivered to the substrate surface as protein clusters of uniform size. This has been confirmed using both AFM and fluorescence microscopy. The application of bioconjugation in this study opens a new avenue for tunable surface modification and controllable protein delivery in studies of biological systems on the nanometer scale.
Yan
Funing
F
Illinois Institute of Technology, Chicago, Illinois 60616, USA.
Chen
Liaohai
L
Tang
Qiling
Q
Wang
Rong
R
D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000528:radiation effects*; D016503:Drug Delivery Systems / Q000379:methods*; D010782:Photolysis; D013499:Surface Properties / Q000528:radiation effects
D003432:Cross-Linking Reagents