Fungerin, a fungal alkaloid, arrests the cell cycle in M phase by inhibition of microtubule polymerization.
A fungal alkaloid fungerin was found to arrest the cell cycle of Jurkat cells at the G2/M phase, then to induce apoptosis. Immunoblotting showed that fungerin led to hyperphosphorylation for Cdc25C and dephosphorylation of Cdc2, indicating that the compound arrests the cell cycle at the M phase. Moreover, fungerin inhibited the polymerization of microtubule proteins in vitro. It was concluded that fungerin arrests the cell cycle at the M phase through inhibition of microtubule polymerization.
Koizumi
Yukio
Y
Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University and The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Arai
Masayoshi
M
Tomoda
Hiroshi
H
Omura
Satoshi
S
D016475:3T3 Cells; D000179:Acrylates / Q000494:pharmacology*; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D006801:Humans; D006999:Hypocreales / Q000737:chemistry*; D007093:Imidazoles / Q000494:pharmacology*; D019169:Jurkat Cells; D051379:Mice; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*
D000179:Acrylates; D000470:Alkaloids; D007093:Imidazoles; C492083:fungerin