Rapid synthesis of [18F]FDG without an evaporation step using an ionic liquid.
In this study, we describe a new 2-[18F]fluoro-2-deoxy-d-glucose ([18F]FDG) synthesis without a distillation step. This involves fluorinating in an ionic liquid-containing medium. A test for the effective elution of [18F]fluoride from the anion exchange resin showed the proper selection of the base and the required eluant composition, which is an essential requirement for the automation of [18F]FDG synthesis. An 18F-labeling study by nucleophilic substitution showed that the major factors controlling the yield were the temperature and the reaction medium composition. The 18F-fluorination proceeded with a labeling efficiency of 74.6+/-7.4% (n=8) for optimized conditions. Alkaline hydrolysis and purification carried out in the liquid phase provided a final decay-corrected [18F]FDG yield of 59.1+/-5.1% (n=3), a radiochemical purity of 91.9+/-3.7% (n=3), and a reaction time of 13 min. Alkaline hydrolysis and purification carried out in the solid phase provided a final decay-corrected [18F]FDG yield of 48.8+/-6.0% (n=3), a radiochemical purity of 96.0+/-4% (n=3), and a reaction time of 19 min. The rapid and straightforward synthesis of [18F]FDG can be achieved by eliminating all evaporation steps, which is made possible by the use of ionic liquid-containing media for the fluorination step.
Kim
Hyung Woo
HW
Department of Nuclear Medicine, Seoul National University College of Medicine, 28 Yungun-dong Chongro-ku, Seoul 110-74, Republic of Korea.
Jeong
Jae Min
JM
Lee
Yun-Sang
YS
Chi
Dae Yoon
DY
Chung
Kyoo-Hyun
KH
Lee
Dong Soo
DS
Chung
June-Key
JK
Lee
Myung Chul
MC
D002852:Chromatography, Ion Exchange / Q000379:methods*; D019788:Fluorodeoxyglucose F18 / Q000138:chemical synthesis* / Q000302:isolation & purification*; D007477:Ions; D007553:Isotope Labeling / Q000379:methods*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000302:isolation & purification; D012996:Solutions; D014835:Volatilization
D007477:Ions; D019275:Radiopharmaceuticals; D012996:Solutions; D019788:Fluorodeoxyglucose F18