Zooming in on a quantitative trait for tomato yield using interspecific introgressions.
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.
Fridman
Eyal
E
Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel.
Carrari
Fernando
F
Liu
Yong-Sheng
YS
Fernie
Alisdair R
AR
Zamir
Dani
D
D000483:Alleles; D000595:Amino Acid Sequence; D001224:Aspartic Acid; D020134:Catalytic Domain; D003433:Crosses, Genetic; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D005638:Fruit / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D032322:Solanum / Q000201:enzymology / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D001224:Aspartic Acid; D013395:Sucrose; D043324:beta-Fructofuranosidase