Functional effects of variants of the RNA chaperone Hfq.
The ring-shaped RNA chaperone Hfq has recently received much attention owing to its multiple roles in RNA metabolism. In this study we have performed a mutational analysis of the Escherichia coli hfq gene, and have studied the effects of amino acid substitutions at several positions in the Hfq protein as well as of C-terminal truncations on its role in phage Qbeta replication, in repression of a target mRNA, and on the stability of the small regulatory RNA DsrA. These functional studies provided insights into the interaction of Hfq with RNA and suggested a role for the C-terminus of Hfq in DsrA stability.
Sonnleitner
Elisabeth
E
Max F. Perutz Laboratories, Department of Microbiology and Genetics, University Departments at the Vienna Biocenter, Dr. Bohrgasse 9/4, 1030 Vienna, Austria.
Napetschnig
Johanna
J
Afonyushkin
Taras
T
Ecker
Karin
K
Vecerek
Branislav
B
Moll
Isabella
I
Kaberdin
Vladimir R
VR
Bläsi
Udo
U
D017908:Allolevivirus / Q000254:growth & development; D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D035001:Host Factor 1 Protein / Q000235:genetics* / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D058727:RNA, Small Untranslated; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
D001425:Bacterial Outer Membrane Proteins; C499660:DrsA protein, E coli; C405431:DsrA RNA, E coli; D029968:Escherichia coli Proteins; C087373:Hfq protein, E coli; D035001:Host Factor 1 Protein; D018832:Molecular Chaperones; D058727:RNA, Small Untranslated; D022661:RNA, Untranslated; D011994:Recombinant Proteins; C078167:OMPA outer membrane proteins; D012313:RNA