title: Isoamyl alcohol-induced morphological change in Saccharomyces cerevisiae involves increases in mitochondria and cell wall chitin content.
abstract: Isoamyl alcohol reduced growth and induced filament formation in Saccharomyces cerevisiae. Isoamyl alcohol-induced filamentation was accompanied by an almost threefold greater increase in the specific activity of succinate dehydrogenase than in untreated cells, which suggested that isoamyl alcohol treatment caused the cells to produce more mitochondria than in normal yeast form proliferation. This was supported by measuring the dry weight of purified, isolated mitochondria. Filaments have an increased chitin content which is distributed over the majority of their surface, and is not confined to bud scars and the chitin ring between mother and daughter cells as in yeast-form cells.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, P.O. Box 915, Cardiff CF10 3TL, UK.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kern
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Nunn
authors.forename: Alena
authors.initials: A
authors.lastname: Pichová
authors.forename: J Richard
authors.initials: JR
authors.lastname: Dickinson
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry; D002473:Cell Wall / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D000439:Pentanols / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D001557:Benzenesulfonates; D000439:Pentanols; D002686:Chitin; C007061:C.I. Fluorescent Brightening Agent 28; C029683:isopentyl alcohol; D013385:Succinate Dehydrogenase