title: Suggestive evidence of association of C-159T functional polymorphism of the CD14 gene with atopic asthma in northern and northwestern Indian populations.
abstract: CD14 is a lipopolysaccharide receptor known to be an important modulator of Th1-Th2 response during early childhood. Genetic association studies of the CD14 gene with asthma and atopic disorders have shown positive as well as negative results in different ethnic populations. The aim of this study was to test for association of C-159T functional promoter polymorphism with atopic asthma and serum IgE levels in northern and northwestern Indian populations. DNA was assayed for the CD14 C-159T polymorphism in a case-control study involving atopic asthmatics (n=187) and healthy normal controls (n=227), and in a family-based association study of 106 trios. The case-control study showed an association at the genotypic (P=0.0146) as well as the allelic level (P=0.0048). Moreover, we observed a deviation of allelic transmission from random proportions (P=0.024) in the transmission disequilibrium test analysis. When we analyzed our results for serum total IgE levels, against this polymorphism, we observed a difference at the genotypic (P=0.0026) as well as at the allelic level (P=0.0016) in a case-control study, whereas no association in the quantitative transmission disequilibrium test analysis was obtained. These findings provide suggestive evidence of association of the CD14 gene locus with atopic asthma in northern and northwestern Indian populations.
authors.affiliation: Molecular Immunogenetics Laboratory, Institute of Genomics and Integrative Biology, Mall Road, 110007, Delhi, India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Sharma
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Batra
authors.forename: U
authors.initials: U
authors.lastname: Mabalirajan
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Goswami
authors.forename: Dipyaman
authors.initials: D
authors.lastname: Ganguly
authors.forename: Brajen
authors.initials: B
authors.lastname: Lahkar
authors.forename: N K
authors.initials: NK
authors.lastname: Bhatia
authors.forename: Amrendra
authors.initials: A
authors.lastname: Kumar
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
mesh_terms: D000328:Adult; D000483:Alleles; D001249:Asthma / Q000097:blood / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D018562:Disease Transmission, Infectious; D005190:Family; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007194:India / Q000453:epidemiology; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics* / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000276:immunology
chemical_list: D018950:Lipopolysaccharide Receptors; D007073:Immunoglobulin E