title: African swine fever virus inhibits induction of the stress-induced proapoptotic transcription factor CHOP/GADD153.
abstract: Stress signaling from mitochondria and the endoplasmic reticulum (ER) leads to the induction of the proapoptotic transcription factor CHOP/GADD153. Many viruses use the ER as a site of replication and/or envelopment, and this activity can lead to the activation of ER stress and apoptosis. African swine fever virus (ASFV) is assembled on the cytoplasmic face of the ER and ultimately enveloped by ER membrane cisternae. The virus also recruits mitochondria to sites of viral replication and induces the mitochondrial stress protein hsp60. Here we studied the effects of ASFV on the induction of CHOP/GADD153 in infected cells. Interestingly, unlike other ER-tropic viruses, ASFV did not activate CHOP and was able to inhibit the induction of CHOP/GADD153 by a number of exogenous stimuli.
authors.affiliation: Division of Immunology, Institute for Animal Health, Pirbright Laboratory, Ash Rd., Pirbright, Surrey GU24 0NF, United Kingdom.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Netherton
authors.forename: James C
authors.initials: JC
authors.lastname: Parsley
authors.forename: Thomas
authors.initials: T
authors.lastname: Wileman
mesh_terms: D000358:African Swine Fever Virus / Q000472:pathogenicity*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism*; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D015398:Signal Transduction; D051742:Transcription Factor CHOP; D014157:Transcription Factors / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D014157:Transcription Factors; D051742:Transcription Factor CHOP