title: Methylobacillus pratensis sp. nov., a novel non-pigmented, aerobic, obligately methylotrophic bacterium isolated from meadow grass.
abstract: Strain F31T was isolated from meadow grass (Poa trivialis L.) sampled from the city park in Helsinki. Analysis of phenotypic and genotypic properties showed the strain to be related to the group of obligately methylotrophic non-methane utilizing bacteria (methylobacteria) with the ribulose monophosphate pathway of formaldehyde assimilation. Phylogenetic analysis showed the strain to be closely related to the genus Methylobacillus, and analysis of fatty acid composition confirmed this association. Thus, on the basis of its genotypic and phenotypic properties, the isolate is proposed as a novel species of the genus Methylobacillus, Methylobacillus pratensis sp. nov., with F31T as the type strain (= VKM B-2247T = NCIMB 13994T).
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow region, Russia.
authors.forename: Nina V
authors.initials: NV
authors.lastname: Doronina
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow region, Russia.
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Trotsenko
authors.affiliation: Bioengineering Center, Russian Academy of Sciences, 117312 Moscow, Russia.
authors.forename: Tatjana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, 117312 Moscow, Russia.
authors.forename: Tatjana P
authors.initials: TP
authors.lastname: Tourova
authors.affiliation: Department of Applied Chemistry and Microbiology, University of Helsinki, PO Box 56, FIN 00014, Finland.
authors.forename: Mirja S
authors.initials: MS
authors.lastname: Salkinoja-Salonen
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004783:Environmental Microbiology; D004798:Enzymes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005387:Finland; D005557:Formaldehyde / Q000378:metabolism; D020459:Genes, rRNA; D020631:Methylobacillus / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010802:Phylogeny; D006109:Poaceae / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005557:Formaldehyde