[Preparation and purification of the antibody against ribonuclease inhibitor].
OBJECTIVE
To prepare and purify the antibody against ribonuclease inhibitor(RI).


METHODS
RI was extracted from human placenta and purified by affinity chromatography. Rabbits anti-RI antibody was obtained after immunization and then purified through rProtein A Sepharose Fast Flow chromatography column. The characters of the antibody was identified by SDS-PAGE, ELISA and Western blot.


RESULTS
An anti-RI antibody was obtained and purified. SDS-PAGE analysis showed that the purified anti-RI antibody has high purity. The results of Western blot and ELISA indicated that anti-RI antibody had high specificity and good stability.


CONCLUSIONS
The anti-RI antibody with high titer, high specificity and good stability has been acquired, which lays the foundation for further research on RI.
Shu
Xiao-hong
XH
Department of Pharmaceutical Chemistry, Dalian Medical University, Dalian 116023, China. schuxh@yahoo.com.cn
Li
Chuan-gang
CG
Zhang
Hai-tao
HT
Chen
Jun-xia
JX
Tian
Yu-xiang
YX
Yu
Li-hua
LH
Cui
Xiu-yun
XY
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D008297:Male; D010920:Placenta / Q000737:chemistry*; D010926:Placental Hormones / Q000276:immunology* / Q000302:isolation & purification; D011247:Pregnancy; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera; D010926:Placental Hormones; C022052:placental ribonuclease inhibitor