title: Interferon alpha (IFN) treatment of bone marrow stroma inhibits haematopoesis.
abstract: Prolonged pre-transplant IFN administration in patients with chronic myeloid leukaemia has been associated with an increased risk of fatal transplant-related complications and an inferior outcome after allogeneic bone marrow transplantation. Using a two-stage long-term bone marrow culture as an in vitro model we were able to show a negative effect of higher doses of IFN pre-treatment of bone marrow stroma on haematopoesis. This correlated with a decline in the number of monocytes-macrophages. While monocytes-macrophages itself produce cytokines, a vicious circle may lead to an ineffective function of the microenvironment resulting in ineffective haematopoieses and increased transplant-related complications.
authors.affiliation: Department of Medicine IV (Haematology and Oncology), Martin-Luther-University, Halle-Wittenberg, Ernst-Grube-Street 40, Halle (Saale) D-06120, Germany. christoph.kasper@web.de
authors.forename: Christoph
authors.initials: C
authors.lastname: Kasper
authors.forename: Anna
authors.initials: A
authors.lastname: Lübking
authors.forename: Dietrich W
authors.initials: DW
authors.lastname: Beelen
authors.forename: Ullrich
authors.initials: U
authors.lastname: Dührsen
mesh_terms: D001853:Bone Marrow / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D017154:Stromal Cells / Q000187:drug effects*
chemical_list: D016898:Interferon-alpha