High-throughput microarray detection of olfactory receptor gene expression in the mouse.
The large number of olfactory receptor genes necessitates high throughput methods to analyze their expression patterns. We have therefore designed a high-density oligonucleotide array containing all known mouse olfactory receptor (OR) and V1R vomeronasal receptor genes. This custom array detected a large number of receptor genes, demonstrating specific expression in the olfactory sensory epithelium for approximately 800 OR genes previously designated as ORs based solely on genomic sequences. The array also enabled us to monitor the spatial and temporal distribution of gene expression for the entire OR family. Interestingly, OR genes showing spatially segregated expression patterns were also segregated on the chromosomes. This correlation between genomic location and spatial expression provides unique insights about the regulation of this large family of genes.
Zhang
Xinmin
X
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Rogers
Matthew
M
Tian
Huikai
H
Zhang
Xiaohong
X
Zou
Dong-Jing
DJ
Liu
Jian
J
Ma
Minghong
M
Shepherd
Gordon M
GM
Firestein
Stuart J
SJ
D000367:Age Factors; D000818:Animals; D002875:Chromosomes / Q000235:genetics; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D016678:Genome; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009831:Olfactory Mucosa / Q000378:metabolism* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018035:Receptors, Odorant / Q000096:biosynthesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism; D019147:Vomeronasal Organ / Q000378:metabolism
D018035:Receptors, Odorant