Mono(ADP-ribosyl)ation of the N2 amino groups of guanine residues in DNA by pierisin-2, from the cabbage butterfly, Pieris brassicae.
Pierisin-2 is a cytotoxic and apoptosis-inducing protein present in Pieris brassicae with a 91% homology in the deduced amino acid sequences to pierisin-1 from Pieris rapae. We earlier showed pierisin-1 to catalyze mono(ADP-ribosyl)ation of 2'-deoxyguanosine (dG) in DNA to form N2-(ADP-ribos-1-yl)-2'-deoxyguanosine, this DNA modification appearing linked to its cytotoxicity and ability to induce apoptosis in mammalian cell lines. In this paper, we documented evidence that pierisin-2 also catalyzed ADP-ribosylation of dG in DNA to give the same reaction product as demonstrated for pierisin-1, with similar efficiency. With oligonucleotides as substrates, ADP-ribosylation by pierisin-2 was suggested to occur by one-side attack of the carbon atom at 1 position of the ribose moiety in NAD toward N2 of dG. The presence of a unique ADP-ribosylation toxin targeting dG in DNA in two distinct species in a Pieris genus could be a quite important finding to better understand biological functions of pierisin-1 and -2 in Pieris butterflies and the generic evolution of these cabbage butterflies.
Takamura-Enya
Takeji
T
Cancer Prevention Basic Research Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
Watanabe
Masahiko
M
Koyama
Kotaro
K
Sugimura
Takashi
T
Wakabayashi
Keiji
K
D036002:ADP Ribose Transferases / Q000737:chemistry; D000246:Adenosine Diphosphate Ribose / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D002080:Butterflies / Q000378:metabolism*; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry*; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013329:Structure-Activity Relationship
D000596:Amino Acids; D019476:Insect Proteins; D046911:Macromolecular Substances; C113557:pierisin protein, insect; D000246:Adenosine Diphosphate Ribose; D006147:Guanine; D004247:DNA; D036002:ADP Ribose Transferases