title: Carbonic anhydrase-related protein VIII: autoantigen in paraneoplastic cerebellar degeneration.
abstract: Serum of a patient with paraneoplastic cerebellar degeneration (PCD) and malignant melanoma had a unique reactivity with Purkinje cells that was not observed in serum of patients with PCD and other tumors, or with malignant melanoma without paraneoplastic syndromes. The screening of a human cerebellar complementary DNA expression library with the patient's serum resulted in the isolation of the CA8 gene. CA8 encodes carbonic anhydrase-related protein (CARP) VIII, preferentially expressed in Purkinje cells. The patient had intrathecal synthesis of CARP VIII antibodies. One of seven melanomas tested expressed CARP VIII. These data suggest CARP VIII may be an autoantigen involved in the pathogenesis of melanoma-associated PCD.
authors.affiliation: Service of Neurology, Hospital Clinic, Universitat de Barcelona and Institut d'Investigació Biomédica August Pi I Sunyer, Barcelona, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Bataller
authors.forename: Lidia
authors.initials: L
authors.lastname: Sabater
authors.forename: Albert
authors.initials: A
authors.lastname: Saiz
authors.forename: Carles
authors.initials: C
authors.lastname: Serra
authors.forename: Berta
authors.initials: B
authors.lastname: Claramonte
authors.forename: Francesc
authors.initials: F
authors.lastname: Graus
mesh_terms: D000368:Aged; D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D014408:Biomarkers, Tumor; D015153:Blotting, Western / Q000379:methods; D002256:Carbonic Anhydrases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020362:Paraneoplastic Cerebellar Degeneration / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D047908:Intracellular Signaling Peptides and Proteins
chemical_list: D001324:Autoantigens; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; D009419:Nerve Tissue Proteins; C512821:Car8 protein, mouse; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins