Genomic analyses of primary and metastatic serous epithelial ovarian cancer.
Epithelial ovarian cancer is the most lethal gynecologic malignancy in the western world. In 75% of patients, peritoneal metastases are found at the time of primary surgery. However, the genetic events leading to the development of ovarian tumors and to the genetic progression toward metastasis remain unclear. To gain insight into this issue, the types and patterns of DNA copy number changes were compared between primary ovarian tumors and their respective metastases by using comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH). The genetic alterations (deletions and amplifications) detected by CGH were similar in the primary tumors and in their respective metastases. Moreover, the FISH results show a similar pattern of chromosomal abnormalities. Our results imply that the major gross genetic changes in ovarian cancer take place in the primary tumor, and the additional genetic changes that may occur in the metastases are not detectable by CGH.
Israeli
Ofir
O
Danek Gertner Institute of Human Genetics, Chaim Sheba Medical Center, Tel Hashomer 52621, Ramat-Gan, Israel.
Gotlieb
Walter H
WH
Friedman
Eitan
E
Korach
Jacob
J
Friedman
Eddy
E
Goldman
Boleslaw
B
Zeltzer
Assaf
A
Zeltser
Assaf
A
Ben-Baruch
Gilad
G
Rienstein
Shlomit
S
Aviram-Goldring
Ayala
A
D002872:Chromosome Deletion; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D010051:Ovarian Neoplasms / Q000235:genetics*; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000556:secondary