Identification of basal promoter and enhancer elements in an untranslated region of the TT virus genome.
The regulation of TT virus (TTV) gene expression was characterized. Transient-transfection assays using reporter constructs revealed that a 113-nucleotide (nt) sequence within the untranslated region, proximal to the transcription initiation site and containing a TATA box motif, has a basal promoter activity. This sequence is well conserved among different TTV genotypes. Upstream stimulating factor bound to a consensus binding motif within this region and positively regulates TTV transcription. Furthermore, a 488-nt region upstream of the basal promoter exhibited enhancer activity, presumably in a cell type-specific manner. This study illustrates some of the mechanisms involved in the transcriptional regulation of TTV.
Suzuki
Tetsuro
T
Department of Virology II, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. tesuzuki@nih.go.jp
Suzuki
Ryosuke
R
Li
Jin
J
Hijikata
Minako
M
Matsuda
Mami
M
Li
Tiang-Cheng
TC
Matsuura
Yoshiharu
Y
Mishiro
Shunji
S
Miyamura
Tatsuo
T
D019677:Artificial Gene Fusion; D001483:Base Sequence; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016385:TATA Box / Q000235:genetics; D022783:Torque teno virus / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D020506:Untranslated Regions; D051720:Upstream Stimulatory Factors
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D020506:Untranslated Regions; D051720:Upstream Stimulatory Factors; D008156:Luciferases