title: Identification of monoclonal anti-HMW-MAA antibody linear peptide epitope by proteomic database mining.
abstract: An efficient strategy is presented for the identification of antigenic sequences in the context of given MHC molecules of interest. The proteomic analysis of the antigenic peptide repertoire is described and demonstrated by using high-molecular weight melanoma-associated antigen. The identification of the epitopic sequence of a monoclonal antibody raised against the 250 kDa tumor associated antigen was reached by using only seven short synthetic peptide fragments, instead of the 155 non-overlapping 15-mer peptides theoretically necessary as minimum screening library. The present result has been obtained by applying as driving criteria the analysis of the peptide affinity to MHC class II molecules and the non-self discrimination concept.
authors.affiliation: Departments of Medicine/Oncology, New York Medical College, Valhalla, New York, USA.
authors.forename: Abraham
authors.initials: A
authors.lastname: Mittelman
authors.forename: Raj
authors.initials: R
authors.lastname: Tiwari
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Lucchese
authors.forename: Jörg
authors.initials: J
authors.lastname: Willers
authors.forename: Reinhard
authors.initials: R
authors.lastname: Dummer
authors.forename: Darja
authors.initials: D
authors.lastname: Kanduc
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D030562:Databases, Protein; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D040901:Proteomics; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D000099216:Chondroitin Sulfate Proteoglycan 4