title: Analysis of hematopoietic progenitor cells in patients with myelodysplastic syndromes according to their cytogenetic abnormalities.
abstract: The present work analyzes the hematopoietic progenitor cells (HPC) in myelodysplastic syndrome (MDS) patients using both an immunophenotypical and a functional approaches in order to know whether they are similar in patients with or without cytogenetic abnormalities. Among CD34+ HPC, the proportion of myeloid committed progenitors was higher in patients with an abnormal karyotype. Ninety MDS patients were studied. Patients with abnormal karyotype showed a similar platting efficiency than patients with normal cytogenetics. Trisomy 8 and 5q- showed a significant higher P.E. than patients with normal karyotype or monosomy 7. We observed that when the most immature HPC were studied, the total number of granulo-monocytic colonies produced by LTBMC was higher in the normal karyotype group. In summary, the present study shows that in MDS the HPC are impaired; this impairment is deeper in patients with abnormal karyotype.
authors.affiliation: Department of Hematology, Hospital Clínico Universitario de Salamanca, Paseo de San Vicente 58-182, 37007, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: López-Holgado
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Arroyo
authors.forename: Carmen
authors.initials: C
authors.lastname: Pata
authors.forename: Eva
authors.initials: E
authors.lastname: Villarón
authors.forename: Fermín
authors.initials: F
authors.lastname: Sánchez Guijo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martín
authors.forename: Jesús María
authors.initials: JM
authors.lastname: Hernández Rivas
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Jesús Fernando
authors.initials: JF
authors.lastname: San Miguel
authors.forename: M Consuelo
authors.initials: MC
authors.lastname: Del Cañizo Fernández-Roldán
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D014314:Trisomy