Albumin-containing sol-gel glasses: chemical and biological study.
Glasses incorporating increasing amounts of bovine serum albumin were prepared by sol-gel techniques from a tetra methoxy silane precursor. The surface of the glass samples was studied by X-ray photoelectron spectroscopy, revealing that the protein is present also in the superficial layer of the silica network. Moreover, the protein is distributed in a dose-dependent way, since the N/Si atomic ratio increases linearly with the albumin concentration in the reaction mixture. Angle-dependent measurements show that the protein distribution occurs homogeneously and is the same at different sampling depths. Protein incorporation in the bulk SiO2 network, with a uniform protein distribution between bulk and surface, is confirmed by infrared spectroscopy measurements, performed both in reflectance and transmittance mode. The reaction with a specific antibody and the adhesivity assay of osteoblastic cells show that embedded albumin present on the glass surface is able to interact with other proteins.
Iucci
G
G
Dipartimento di Fisica E. Amaldi, Università "Roma Tre" ed Unità INFM, INSTM, Via della Vasca Navale 84, 00146 Roma, Italy. iucci@uniroma3.it
Infante
G
G
Rossi
L
L
Polzonetti
G
G
Rosato
N
N
Avigliano
L
L
Savini
I
I
Catani
M V
MV
Palacios
A C
AC
D000327:Adsorption; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D044367:Phase Transition; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005782:Gels; D012710:Serum Albumin, Bovine