Crystallization of GcnA, an N-acetyl-beta-D-glucosaminidase, from Streptococcus gordonii.
Streptococcus gordonii is a primary colonizer of the surface of human teeth. The gcnA gene is one of a number of genes involved in glycoside metabolism. GcnA has N-acetyl-beta-D-glucosaminidase (EC 3.2.1.52) activity; it has been overexpressed, purified and crystallized. Diffraction has been observed to beyond 1.5 A resolution and synchrotron data have been recorded to 1.55 A resolution. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 112.5, b = 104.0, c = 110.0 A. The crystals contain either a monomer or a dimer in the asymmetric unit.
Langley
David B
DB
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
Harty
Derek W S
DW
Graham
Stephen C
SC
Guss
J Mitchell
JM
Hunter
Neil
N
Collyer
Charles
C
D000118:Acetylglucosaminidase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D009991:Oscillometry; D013291:Streptococcus / Q000201:enzymology*; D013696:Temperature
D000118:Acetylglucosaminidase