Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1.
We investigated whether CD4+ T cells that do not produce HIV-1 are killed in HIV-infected human lymphoid tissue. Tissue blocks were inoculated with high amount of doxycycline-dependent HIV-rtTA. Doxycycline triggered productive infection and loss of CD4+ T cells in these tissues, whereas without doxycycline, neither productive infection nor CD4+ T cell depletion was detected in spite of the massive presence of virions in the tissue and of viral DNA in the cells. Thus, HIV-1 alone is sufficient to deplete productively infected CD4+ T cells but is not sufficient to cause the death of uninfected or latently infected CD4+ T cells.
Kiselyeva
Yana
Y
Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
Ito
Yoshinori
Y
Lima
Rosangela G
RG
Grivel
Jean-Charles
JC
Das
Atze T
AT
Berkhout
Ben
B
Margolis
Leonid B
LB
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004318:Doxycycline / Q000494:pharmacology*; D015658:HIV Infections / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000472:pathogenicity*; D006801:Humans; D008212:Lymphocyte Depletion; D008221:Lymphoid Tissue / Q000187:drug effects* / Q000276:immunology* / Q000821:virology; D013997:Time Factors; D014774:Virulence / Q000187:drug effects; D017735:Virus Latency
D000890:Anti-Infective Agents; D004318:Doxycycline