Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection.
Determination of quercetin in human plasma was carried out by semi-micro high-performance liquid chromatography with electrochemical detection. Peak heights for quercetin were found to be linearly related to the amount of each quercetin injected from 1.5 to 750 pg. The detection limit (signal-noise ratio, S/N = 3) of the present method for quercetin was 0.3 pg. Glucuronic and sulfate forms of quercetin in plasma were hydrolyzed enzymatically using beta-glucuronidase and sulfatase, respectively. Quercetin in plasma and the hydrolyzed solution were extracted with ethyl acetate and determined by the present method. The time courses of concentrations of quercetin in human plasma showed maxima at 1-1.5 h after ingestion of 340 mL of commercial canned green tea.
Jin
Dongri
D
Chemistry Department of Science and Engineering College, Yanbian University, 105 Gongyuan Rd, Yanji 133002, People's Republic of China.
Hakamata
Hideki
H
Takahashi
Kouji
K
Kotani
Akira
A
Kusu
Fumiyo
F
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D008297:Male; D011794:Quercetin / Q000097:blood*; D013662:Tea
D013662:Tea; D011794:Quercetin