[Changes of signal transducer and transcriptional activator 5 (STAT5) in proliferative splenocytes from asthma mice induced by ovalbumin].
OBJECTIVE
To investigate the changes of signal transducer and transcriptional activator 5 (STAT5) in proliferative splenocyte of asthma mice.


METHODS
BALB/c mice were induced to develop asthma by intraperitoneal injection and then nebulized aspiration of OVA. The splenocytes were isolated, and the proliferation of the splenocytes was detected by MTT colorimetry. The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).


RESULTS
OVA could pronouncedly induce the splenocyte proliferation of asthma mice in dose-dependent manner. Stimulation with OVA for 1 and 3 hours could lead to phosphorylation of STAT5 in splenocytes from asthma mice and increase the binding of STAT5 with DNA, suggesting that STAT5 signal pathway plays an important role in splenocyte proliferation of asthma mice induced with OVA.
Li
Guo-ping
GP
College of Life Sciences, Shenzhen University, Shenzhen 518060, China.
Liu
Zhi-gang
ZG
Ran
Pei-xin
PX
Qiu
Jin
J
Zhong
Nan-shan
NS
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D010766:Phosphorylation; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism / Q000473:pathology*
D004268:DNA-Binding Proteins; D050799:STAT5 Transcription Factor; D010047:Ovalbumin; D004247:DNA