title: Dissemination of Escherichia coli producing AmpC-type beta-lactamase (CMY-11) in Korea.
abstract: Among the 51 clinical isolates collected from a university hospital in Korea, nine isolates were resistant to cephamycins. Nine isolates were shown to produce CMY-11 and these also included three isolates producing TEM-1. The results from ERIC-PCR revealed that dissemination of CMY-11 was due to outbreaks of resistant species and to the intra-species spread of resistance to cephamycins in Korea. CMY-11 beta-lactamase genes from nine clinical isolates that were responsible for resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin and amoxicillin-clavulanic acid, were cloned and characterised. A sequence identical to the common regions in In6, In7 and a novel integron from pSAL-1 was found upstream from bla(CMY-11) gene at nucleotide 1-71. Eighteen nucleotides between position 71 and 72 were inserted into the bla(CMY-11) gene.
authors.affiliation: Bio Technology Innovation Centre, Youngdong University, Chungbuk 370-701, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jae Seok
authors.initials: JS
authors.lastname: Song
authors.forename: Song Hee
authors.initials: SH
authors.lastname: Bak
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Cho
authors.forename: Dae Wi
authors.initials: DW
authors.lastname: Kim
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
authors.forename: Kye Joon
authors.initials: KJ
authors.lastname: Lee
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D007723:Korea; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C048795:AmpC beta-lactamases; D001618:beta-Lactamases