title: Triterpene saponins from the roots of Clematis chinensis.
abstract: A saponin-enriched fraction prepared from the MeOH extract of the roots of Clematis chinensis showed cytotoxic activity against HL-60 promyelocytic leukemia cells, from which five new triterpene saponins based on oleanolic acid, along with three known saponins, were isolated. The structures of the new saponins were determined on the basis of spectroscopic analysis, including extensive 1D and 2D NMR data and hydrolysis followed by chromatographic and spectroscopic analysis. Among the isolated saponins, monodesmosidic saponins exhibited cytotoxic activities against cultured tumor cells.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan. mimakiy@ps.toyaku.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mimaki
authors.forename: Akihito
authors.initials: A
authors.lastname: Yokosuka
authors.forename: Mari
authors.initials: M
authors.lastname: Hamanaka
authors.forename: Chiseko
authors.initials: C
authors.lastname: Sakuma
authors.forename: Takao
authors.initials: T
authors.lastname: Yamori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sashida
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D039324:Clematis / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D012503:Saponins; D009828:Oleanolic Acid