[Preparation of bispecific monoclonal antibody against HIV p24 and human group A erythrocytes].
OBJECTIVE
To prepare bispecific monoclonal antibody (bsmAb) against HIV p24 and human group A erythrocytes, and set up an indirect hemagglutination test for detecting HIV p24.


METHODS
Hybridoma cells 2-E(4) secreting anti-HIV p24 mAb and hybridoma cells S(2) secreting anti-human group A erythrocyte mAb were naturalized with 8-Ag and 5-BrdU respectively, making them sensitive to HAT. Then the two hybridoma cells sensitive to HAT were fused by routine method and hybrid-hybridoma cells secreting the bsmAb against HIV p24 and human group A erythrocytes were screened. The bsmAb was used to establish an indirect hemagglutination test for detecting HIV p24.


RESULTS
6 hybrid-hybridoma cell lines were obtained. An indirect hemagglutination test for detecting HIV p24 was set up by using the bsmAb, and its sensitivity reached 400 ng/L.


CONCLUSIONS
The bsmAb against HIV p24 and human group A erythrocytes is prepared and a rapid indirect hemagglutination test for detecting HIV p24 is developed by using the purified bsmAb.
Meng
Wen-xia
WX
Department of Immunology, Institute of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China.
Wang
Run-tian
RT
Zhang
Yu-fen
YF
Zhang
Hong-zhong
HZ
Wang
Hui-fen
HF
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D018033:Antibodies, Bispecific / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D016655:HIV Core Protein p24 / Q000276:immunology*; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013696:Temperature
D000017:ABO Blood-Group System; D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D016655:HIV Core Protein p24