title: Usefulness of bone marrow aspiration for definite diagnosis of Asian variant of intravascular lymphoma: four autopsied cases.
abstract: The Asian variant of intravascular lymphoma (AIVL) is characterized by hemophagocytic syndrome, pancytopenia and hepatosplenomegaly but usually lacks any neurological abnormality and skin lesions, which are typical features of classical intravascular lymphoma (IVL). An ante-mortem diagnosis of AIVL is difficult due to the absence of visible lymphoma lesions and unspecific clinical manifestations. A definite diagnosis relies on the presence of neoplastic B cells in the lumina of small vessels. Paraffin block samples of aspirated bone marrow clots were obtained from 4 patients with clinically suspected IVL and subjected to immunohistopathological analysis. All samples exhibited CD 20+ or CD 79a+ lymphoma cells proliferating intravascularly as well as erythrocytic hemophagocytosis. The distribution of neoplastic cells in the structure of the bone marrow allowed IVL to be distinguished from bone marrow invasions due to other types of lymphoma. We demonstrated the successful establishment of a definite ante-mortem diagnosis of AIVL in 3 of 4 patients by the rapid and simple method of using aspirated bone marrow samples.
authors.affiliation: Department of Hematology and Oncology, JA Aichi Showa Hospital, Konan, Japan. hiro-na@d4.dion.ne.jp
authors.forename: Hiroto
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Morishita
authors.forename: Shigeki
authors.initials: S
authors.lastname: Saito
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Shimada
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Ozeki
authors.forename: Akio
authors.initials: A
authors.lastname: Kohno
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Nagasaka
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism; D001208:Asia / Q000208:ethnology; D044466:Asian People; D001344:Autopsy; D001854:Bone Marrow Cells / Q000473:pathology*; D001856:Bone Marrow Examination; D051925:CD79 Antigens; D020732:Cytogenetic Analysis; D005260:Female; D015616:Histiocytosis, Non-Langerhans-Cell; D006801:Humans; D008099:Liver / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000208:ethnology / Q000235:genetics; D008297:Male; D010198:Pancytopenia; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013163:Splenomegaly; D019043:Vascular Neoplasms / Q000145:classification / Q000175:diagnosis* / Q000235:genetics
chemical_list: D015703:Antigens, CD; D018951:Antigens, CD20; D051925:CD79 Antigens; C497881:CD79A protein, human; D011947:Receptors, Antigen, B-Cell