Characterization and mapping of canine microsatellites isolated from BAC clones harbouring DNA sequences homologous to seven human genes.
Human primers specific for the genes LEP, HBB, PAX3, ESR2, TPH1, ABCA4 and ATP2A2 were used to identify clones in a canine BAC library. Subcloning of the positive BACs in plasmids, screening with microsatellite motifs and subsequent sequencing allowed for the identification of eight novel microsatellites. The presence of the gene of interest was confirmed by sequencing the polymerase chain reaction (PCR) products amplified in the positive BACs. Fluorescent in situ hybridization (FISH) using the positive BACs as probes allowed for the chromosomal localization of the insert DNAs in two canid species, dog (Canis familiaris) and red fox (Vulpes vulpes). The use of gene-associated microsatellites may accelerate the identification of candidate genes for phenotypic traits in linkage studies.
Klukowska
J
J
Institute of Animal Genetics, Nutrition and Housing, University of Berne, Vetsuisse-Faculty Bern, Switzerland. jolanta.klukowska@itz.unibe.ch
Szczerbal
I
I
Wengi-Piasecka
A
A
Switonski
M
M
Schelling
C
C
Gmür
A
A
Dolf
G
G
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D005589:Foxes / Q000235:genetics*; D005796:Genes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D017931:DNA Primers