title: Bisphenol A from dental polycarbonate crown upregulates the expression of hTERT.
abstract: Bisphenol A (BPA) is one of the endocrine-disrupting chemicals (EDCs) that possess estrogen-like biologic activity. Many dental materials have been reported to release BPA. However, there are few reports available on the release of BPA from dental polycarbonates. The purpose of this study was to investigate the release of BPA from dental polycarbonate crowns and to evaluate the estrogenic activity of BPA. Polycarbonate crowns were immersed in five solvents (water, ethanol, n-hepthane, acetic acid, and acetonitrile) at 37 or 65 degrees C for 24 h. The elution from the material was analyzed by high-performance liquid-chromatography (HPLC) and mass-spectrometry (MS) analysis. BPA release was detected corresponding to the degradation of dental polycarbonates under the some storage conditions (ethanol, acetic acid, and acetonitrile). A previous report proved that estrogen increased human telomerase catalytic subunit (hTERT) mRNA, whereas the effect of EDCs on the hTERT promoter has never been reported. The estrogenic activity of BPA was analyzed by luciferase assay with the use of the hTERT promoter. This assay revealed that BPA was a positive regulator of hTERT transcription. In addition, quantitative real-time PCR analysis showed that BPA increased the expression level of hTERT mRNA in MCF7 cells. Herein, it is demonstrated that hTERT is a new target of BPA.
authors.affiliation: Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Takahashi
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Higashino
authors.forename: Mariko
authors.initials: M
authors.lastname: Aoyagi
authors.forename: Satoru
authors.initials: S
authors.lastname: Kyo
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakata
authors.forename: Mamoru
authors.initials: M
authors.lastname: Noda
authors.forename: Masanobu
authors.initials: M
authors.lastname: Shindoh
authors.forename: Takao
authors.initials: T
authors.lastname: Kohgo
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Sano
mesh_terms: D001559:Benzhydryl Compounds; D002460:Cell Line; D003442:Crowns; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics*; D019228:Tooth Crown; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004268:DNA-Binding Proteins; D008156:Luciferases; D010636:Phenols; D019098:Telomerase; C006780:bisphenol A; D000098826:Bisphenol A Compounds