title: Histone deacetylase 1 (HDAC-1) required for the normal formation of craniofacial cartilage and pectoral fins of the zebrafish.
abstract: Histone deacetylases interact with nucleosomes to facilitate the formation of transcriptionally repressed chromatin. In the present study, we show that histone deacetylase 1 (hdac-1) is expressed throughout embryonic development of the zebrafish. The expression of hdac-1 is ubiquitous in early embryos (2-16 hr postfertilization), but at later stages (36 and 48 hr postfertilization), it is primarily restricted to the branchial arches, fin bud mesenchyme, and hindbrain. We report the phenotypes of hdac-1 homozygous mutant embryos and embryos injected with an hdac-1 antisense morpholino. These embryos possess a complex phenotype affecting several embryonic structures. We observed developmental abnormalities in the heart and neural epithelial structures, including the retina and the loss of craniofacial cartilage and pectoral fins.
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5.
authors.forename: Renjitha
authors.initials: R
authors.lastname: Pillai
authors.forename: Louise E
authors.initials: LE
authors.lastname: Coverdale
authors.forename: Gaytri
authors.initials: G
authors.lastname: Dubey
authors.forename: C Cristofre
authors.initials: CC
authors.lastname: Martin
mesh_terms: D000423:Alcian Blue / Q000378:metabolism; D000818:Animals; D002356:Cartilage / Q000196:embryology*; D004622:Embryo, Mammalian / Q000187:drug effects; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism / Q000502:physiology*; D006720:Homozygote; D017403:In Situ Hybridization; D016254:Mutagenesis, Insertional; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012886:Skull / Q000196:embryology*; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins; C488363:HDAC1 protein, zebrafish; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D000423:Alcian Blue