Variations in the level of enzyme activity and immunolocalization of calcium-dependent protein kinases in the phloem of different cucumber organs.
Calcium-dependent protein kinases (CDPKs) constitute a unique family of enzymes in plants that are characterized by a C-terminal calmodulin (CaM)-like domain. Through protein kinase assays, we have examined the levels of cucumber calcium-dependent kinase (CsCDPK) activity in various organs of cucumber seedlings and plants. The activity of CsCDPK was highest in cucumber plant leaves followed by seedling roots and hypocotyls; however, cucumber plant flowers, seedling cotyledons, and hooks had levels that were barely detectable. The CsCDPKs were immunolocalized using polyclonal antibodies that are highly specific against a part of the kinase domain of a calcium-dependent protein kinase (CsCDPKS) in the phloem sieve elements (SEs) in various organs of cucumber. In addition, this study indicates the presence of CsCDPKs in organelle-like bodies associated with the plasma membrane of sieve elements in mature stems and roots as well as in the storage bodies of immature seeds. These findings are discussed in terms of the likely roles played by CDPKs in the signal transduction pathways for Ca2+-regulated phloem transport of assimilates from leaves to various organs during growth and development of cucumber seedlings and plants.
Kumar
K G Suresh
KG
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
Jayabaskaran
Chelliah
C
D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D018553:Cucumis sativus / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D007118:Immunoassay; D007700:Kinetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology
D010940:Plant Proteins; D011494:Protein Kinases