Staphylococcus aureus isolates associated with necrotizing pneumonia bind to basement membrane type I and IV collagens and laminin.
To investigate how Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus (PPSA) strains associate with specific bronchial lesions during community-acquired necrotizing pneumonia, we examined PPSA strains and PVL-negative S. aureus (PNSA) strains for their binding behavior to extracellular matrix (ECM) proteins, primary human airway epithelial cell (HAEC) cultures, and human airway mucosa damaged ex vivo. Compared with PNSA strains, PPSA strains exhibited increased affinity for damaged airway epithelium and especially for exposed basement membrane. PPSA strains, compared with PNSA strains, showed stronger affinity for type I and IV collagens and laminin, a property associated with the presence of the cna gene. PPSA and PNSA culture supernatants similarly damaged HAEC layers, whereas recombinant PVL had no effect, suggesting that an S. aureus exoprotein other than PVL might contribute to the observed airway epithelial damage. These results suggest that epithelial damage, possibly due to viral infection (which usually precedes necrotizing pneumonia) and/or to a non-PVL S. aureus exoproduct action, may permit binding of PPSA to exposed type I and IV collagens and laminin--the PVL cytotoxin being involved later during necrotizing pneumonia.
de Bentzmann
Sophie
S
Laboratoire d'Ingenierie des Systemes Macromoleculaires, Unite Propre de Recherche 9027, Marseille, France.
Tristan
Anne
A
Etienne
Jerome
J
Brousse
Nicole
N
Vandenesch
Francois
F
Lina
Gerard
G
D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion; D001427:Bacterial Toxins; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D007956:Leukocidins / Q000235:genetics / Q000378:metabolism; D009336:Necrosis; D018410:Pneumonia, Bacterial / Q000382:microbiology* / Q000473:pathology; D011347:Procollagen / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D018829:Adhesins, Bacterial; D001427:Bacterial Toxins; D024042:Collagen Type I; D024141:Collagen Type IV; D017077:Culture Media, Conditioned; D005098:Exotoxins; D007797:Laminin; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D011347:Procollagen; C048165:adhesin, Staphylococcus aureus