Production of amino acids by Rhizobium, Mesorhizobium and Sinorhizobium strains in chemically defined media.
Five strains of Rhizobium spp, one strain of Mesorhizobium loti and two strains of Sinorhizobium meliloti were tested for their ability to grow in chemically-defined medium lacking growth factor. Qualitative and quantitative production of aspartic acid, serine, glutamic acid, glycine, histidine, threonine, arginine, alanine, proline, cysteine, tyrosine, valine, methionine, lysine, isoleucine, leucine, and phenylalanine was determined by the use of mannitol as sole carbon source. Strains of Rhizobium spp. and Sinorhizobium sp. produced all the amino acids analysed with the exception of cysteine and high biological levels of serine, glycine and alanine were detected after 2 days of culture in mineral medium. Strain U226 of M. loti only produced small amounts of amino acids and glutamic acid, histidine, arginine, cysteine, methionine, lysine and phenylalanine was not liberated into the media.
Salmeron-Lopez
V
V
Department of Microbiology, Faculty of Pharmacy, University of Granada, Spain.
Martinez-Toledo
M V
MV
Salmeron-Miron
V
V
Pozo
C
C
Gonzalez-Lopez
J
J
D000596:Amino Acids / Q000096:biosynthesis / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D003470:Culture Media / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry; D020560:Proteobacteria / Q000378:metabolism*; D012231:Rhizobium / Q000378:metabolism*; D020579:Sinorhizobium / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
D000596:Amino Acids; D003470:Culture Media; D018698:Glutamic Acid; D002244:Carbon