title: Root canal sealers induce cytotoxicity and necrosis.
abstract: There are three types of the root canal sealers commonly used in clinical applications. They are calcium hydroxide base (Sealapex), zinc oxide-eugenol base (Canals), and epoxy-resin base (AH Plus). Elutable substances and degradation products from root canal sealers may gain access to periodontal tissue in a number of ways. The purpose of the present study was to evaluate the biologic effects of the root canal sealers on human oral cancer cell line (OC2). The tetrazolium bromide (MTT) assay was to evaluate the cell's survival rate. The DNA electrophoresis was used to evaluate the OC2 cell's DNA damage. The results demonstrated that the above root canal sealers' survival rates are in dose-dependent increase (p < 0.05). The toxicity of fresh mix group is higher than that of the mixed after 24h group. DNA fragmentation assay of sealer treated OC2 cells shows a smear layer pattern on the electrophoresis gel. There is no DNA damage found. The toxicity that regulated the cell death is not by the apoptic change of cells.
authors.affiliation: Dental Department, Institute of Medicine, Chun Shan Medical Hospital, Chung Shan Medical University, Taichung 402, Taiwan, ROC.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.forename: Shinn-Jyh
authors.initials: SJ
authors.lastname: Ding
authors.forename: Ting-Zen
authors.initials: TZ
authors.lastname: Hsu
authors.forename: Zen-Dar
authors.initials: ZD
authors.lastname: Lee
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002126:Calcium Hydroxide / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D009062:Mouth Neoplasms; D009336:Necrosis; D010895:Pit and Fissure Sealants / Q000737:chemistry / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012459:Salicylates / Q000633:toxicity*
chemical_list: D010895:Pit and Fissure Sealants; D012387:Root Canal Filling Materials; D012459:Salicylates; C055601:Sealapex; D002126:Calcium Hydroxide