Isolation, purification, and crystallization of aspartate aminotransferase from wheat grain.
A procedure for isolation and purification of aspartate aminotransferase from wheat grain includes chromatography on DEAE cellulose, acidification-alkalization, precipitation with protamine sulfate, fractionation with ammonium sulfate, and chromatography on hydroxyapatite. The yield of protein was 27% with 95% purity. Crystals of the enzyme (0.05 x 0.025 x 0.015 mm3) were obtained from ammonium sulfate solution.
Kochkina
V M
VM
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia. belj@eimb.ru
D001219:Aspartate Aminotransferases / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D014908:Triticum / Q000201:enzymology*
D001219:Aspartate Aminotransferases