A new compact disc format of high density array synthesis applied to peptide nucleic acids and in situ MALDI analysis.
A fully automated synthesizer was constructed and designed to perform high speed miniaturized syntheses of compound libraries using the SPOT technique. Utilizing magnetically controlled drop-on-demand ink jet nozzles, an r/phi array format of 2500 spots can be simultaneously dispensed from up to 24 separate reagent valves onto a rotating disc as the solid phase in less than three minutes. In addition, a complete wash station is on board allowing for fully programmable combinatorial syntheses without manual attention. A new carbon black/polypropylene composite solid phase disc was developed and tested for its functionalisation/loading, spot detection, durability and MALDI-TOF target capabilities. The carbon black/polypropylene composite was then successfully employed jointly as the solid phase in the syntheses of short peptide and PNA oligomers and as the target probe holder for MALDI-TOF measurement without transfer of the material. Several protocols for PNA syntheses were also investigated and an optimised PNA methodology for the carbon black/polypropylene composite is reported.
Dikmans
Antonius J
AJ
Research Group for Molecular Recognition, GBF (German Research Centre for Biotechnology), Mascheroder Weg 1, 38124 Braunschweig, Germany.
Morr
Michael
M
Zander
Norbert
N
Adler
Frank
F
Türk
Gerhard
G
Frank
Ronald
R
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007202:Indicators and Reagents; D010455:Peptides; D011092:Polyethylene Glycols