[Effect of KyoT2 binding protein KBP1 on RBP-J-mediated transcriptional activity].
OBJECTIVE
To investigate the physical interaction between KyoT2 and KyoT2-binding protein 1 (KBP1) and the effect of KBP1 on RBP-J-mediated transcriptional activity.


METHODS
GST-pull down, co-immunoprecipitation, mammalian cell two hybrid assay and reporter gene assay were used to examine the physical and functional interactions between KyoT2 and KBP1.


RESULTS
KBP1 and KyoT2 could interact with each other both in-vitro and in-vivo, and overexpression of KBP1 could antagonize the suppressive effect of RING1 on RBP-J.


CONCLUSIONS
KBP1 may indirectly modulate Notch signaling pathway by competing with RING1 for binding sites on KyoT2.
Qin
Hong-yan
HY
Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an 710032, China.
Han
Hua
H
D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000166:cytology*; D060588:LIM Domain Proteins; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D063150:Polycomb Repressive Complex 1; D051880:Receptors, Notch / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C101149:FHL1 protein, human; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; D051880:Receptors, Notch; D011994:Recombinant Proteins; D063150:Polycomb Repressive Complex 1; C080355:RING1 protein, human