title: Interactions of aflatoxin B1 with SRP components can disrupt protein targeting.
abstract: Spectrofluorimetric studies have revealed that aflatoxin B1 (AFB1) interacts with signal recognition particle (SRP), which acts as an escort for polyribosomes with signal peptides to be transported and bound to the cytoplasmic face of the endoplasmic reticulum (ER). We further report that the binding of AFB1 to SRP is selective as it only binds to two (SRP9 and 14) out of its three constituent polypeptides studied. Binding of AFB1 to proteins is known to alter their conformations. Interactions of AFB1 with SRP polypeptides may generate structural and functional alterations in this particle and hinder secretory protein synthesis.
authors.affiliation: Department of Biochemistry, Kurukshetra University, Kurukshetra-136119, India.
authors.forename: Jasbir
authors.initials: J
authors.lastname: Singh
authors.forename: Suman
authors.initials: S
authors.lastname: Singh
authors.forename: Harinder M
authors.initials: HM
authors.lastname: Dani
authors.forename: Rajeshwar
authors.initials: R
authors.lastname: Sharma
authors.forename: Pablo
authors.initials: P
authors.lastname: Steinberg
mesh_terms: D016604:Aflatoxin B1 / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005818:Genetic Engineering; D006801:Humans; D010179:Pancreas / Q000737:chemistry; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D018271:Signal Recognition Particle / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D010455:Peptides; D011506:Proteins; D018271:Signal Recognition Particle; D016604:Aflatoxin B1