Tissue oxygenation in sepsis; new insights from in vivo EPR.
Nitric oxide (NO) is a key mediator in the maldistribution of oxygen by tissue and organ dysfunction observed in sepsis. Despite this, few techniques are capable of measuring these parameters directly in vivo. We describe here several techniques that have been developed by our group to address this directly by in vivo EPR in animal models of sepsis. Oxygen-sensitive materials can be implanted or administered and report on local tissue pO2. Spin trapping of NO can simultaneously report on tissue NO content. Repeat measures of these parameters can be made directly from a defined tissue site, allowing development of new models and experiments to study the defects in tissue and organ function seen in sepsis.
James
Philip E
PE
Department of Cardiology, Wales Heart Research Institute, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK. Jamespp@Cardiff.ac.uk
Thomas
Matthew P
MP
Jackson
Simon K
SK
D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism*; D004195:Disease Models, Animal; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods* / Q000639:trends; D006801:Humans; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D018805:Sepsis / Q000175:diagnosis* / Q000378:metabolism*
D015415:Biomarkers; D009569:Nitric Oxide; D010100:Oxygen