Differentiation of Actinobacillus pleuropneumoniae by PCR-REA based on sequence variability of the apxIVA gene and by ribotyping.
During the period of 2001-2003, a total of 591 Actinobacillus pleuropneumoniae field isolates from the Czech Republic were serotyped with a high occurrence of cross-reactions. The cross-reactions were observed in 416 isolates. Most frequently, in 401 isolates (67.9%), cross-reactions with antisera specific for serotypes 9, 11, and/or 1 were observed. Two additional molecular methods, ribotyping and restriction analysis of PCR amplified apxIVA gene (PCR-REA), were therefore used for detailed characterisation of A. pleuropneumoniae. In this subsequent analysis, reference strains representing serotypes 1-12 and 25 field isolates showing the most frequent serotype cross-reactions were examined. PCR-REA enabled all reference strains to be distinguished except for the strains of serotypes 9 and 11. Ribotyping distinguished all reference strains except two pairs of serotypes: 3 versus 6, and 9 versus 11, respectively. Field isolates with serotype cross-reactivity 9, 11, and/or 1 could not be differentiated by either of these methods.
Jaglic
Zoran
Z
Veterinary Research Institute Brno, Hudcova 70, 621 32 Brno, Czech Republic. jaglic@vri.cz
Svastova
Petra
P
Rychlik
Ivan
I
Nedbalcova
Katerina
K
Kucerova
Zdenka
Z
Pavlik
Ivo
I
Bartos
Milan
M
D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003429:Cross Reactions; D018153:Czech Republic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015183:Restriction Mapping / Q000662:veterinary; D021521:Ribotyping / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D004269:DNA, Bacterial