title: Development of a gas chromatography/ion trap mass spectrometry based method for the quantification of polybrominated diphenyl ethers and polychlorinated biphenyls in adipose tissue.
abstract: A combined gas chromatographic mass spectrometric (GC/MS/MS) method for the determination of seven polybrominated diphenyl ethers (PBDEs) and seven marker polychlorinated biphenyls (PCBs) in adipose tissue has been developed. Adipose tissue was melted and filtered through anhydrous sodium sulphate to obtain pure fat. Clean-up was performed using a glass column containing acidified silica, deactivated alumina and anhydrous sodium sulphate. Polybrominated biphenyl (PBB) 155 and Mirex were added as internal standards for PBDEs and PCBs, respectively. Injection standards, PBB 103 and PCB 143, for PBDEs and PCBs, respectively, were added before analysis with GC/MS/MS. The developed GC/MS/MS method has the advantage of being more selective than single MS methods because matrix effects are largely eliminated. Validation of this method was conducted according to Commission Decision 2002/657/EC. Decision limits for PBDEs and PCBs ranged from 0.06-0.15 ng g(-1) and from 0.35-1.22 ng g(-1), respectively. Detection capabilities were all between 0.23-0.55 ng g(-1) for PBDEs and between 0.98-2.29 ng g(-1) for PCBs. Precision, recovery, bias and selectivity were tested, with satisfactory results.
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium. Caroline.Naert@UGent.be
authors.forename: Caroline
authors.initials: C
authors.lastname: Naert
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.forename: Carlos
authors.initials: C
authors.lastname: Van Peteghem
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001530:Belgium; D002849:Chromatography, Gas / Q000379:methods* / Q000592:standards; D004785:Environmental Pollutants / Q000032:analysis; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D011075:Polybrominated Biphenyls / Q000032:analysis* / Q000145:classification; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000145:classification; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards
chemical_list: D004785:Environmental Pollutants; D011075:Polybrominated Biphenyls; D011078:Polychlorinated Biphenyls