title: Newly Initiated RNA encounters a factor involved in splicing immediately upon emerging from within RNA polymerase II.
abstract: We employed RNA-protein cross-linking to map the path of the nascent RNA as it emerges from within RNA polymerase II. A UV-cross-linkable uridine analog was incorporated at two positions within the first five nucleotides of the transcript. Only the two largest subunits of RNA polymerase II cross-linked to the transcript in complexes containing 17-24-nucleotide (nt) RNAs. Extension of the RNA to 26 or 28 nt revealed an additional strong cross-link to the splicing factor U2AF65. In U17 complexes, in which the RNA is still contained within the polymerase, U2AF65 is tightly bound. In contrast, U2AF65 is more loosely bound in C28 transcription complexes, in which about 10 nt of transcript have emerged from the RNA polymerase. Cross-linking of U2AF65 to RNA in a C28 complex was eliminated by the addition of an excess of an RNA oligonucleotide containing the consensus U2AF65 binding site, but U2AF65 was not displaced by a nonconsensus RNA. These findings indicate that U2AF65 shifts from protein-protein to protein-RNA interactions as the RNA emerges from the polymerase. During transcription of one particular template at low UTP concentration, RNA polymerase II pauses just after synthesizing a transcript segment that is a U2AF65 binding site. Dwell time of the polymerase at this pause site was significantly and specifically reduced by the addition of recombinant U2AF65 to the transcription reaction. Therefore, the association of U2AF65 with RNA polymerase II may function not only to deliver U2AF65 to the nascent transcript but also to modulate efficient transcript elongation.
authors.affiliation: Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ujvári
authors.forename: Donal S
authors.initials: DS
authors.lastname: Luse
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011233:Precipitin Tests; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D012326:RNA Splicing / Q000502:physiology*; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D000072668:Splicing Factor U2AF; D013997:Time Factors; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607494:U2AF2 protein, human; D012313:RNA; D012319:RNA Polymerase II