Extracellular recordings from patterned neuronal networks using planar microelectrode arrays.
Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.
James
Conrad D
CD
Cornell University, Ithaca, NY 14850 USA. cdjame@sandia.gov
Spence
Andrew J H
AJ
Dowell-Mesfin
Natalie M
NM
Hussain
Rifat J
RJ
Smith
Karen L
KL
Craighead
Harold G
HG
Isaacson
Michael S
MS
Shain
William
W
Turner
James N
JN
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005110:Extracellular Space / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000502:physiology; D008839:Microelectrodes; D009415:Nerve Net / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials