A method for the rapid depletion of albumin and immunoglobulin from human plasma.
In studies of the plasma proteome, the high abundance of proteins such as albumin and immunoglobulin impedes the investigation of lower abundance proteins that may be more suitable as biomarkers of disease. We report the specific removal of 98% of albumin and 80% of immunoglobulin heavy chain from human plasma by affinity chromatography, and the subsequent improvement in the number of spots detected and their resolution following two-dimensional gel electrophoresis.
Greenough
Carrie
C
Department of Pharmacology, University of Liverpool, UK.
Jenkins
Rosalind E
RE
Kitteringham
Neil R
NR
Pirmohamed
Munir
M
Park
B Kevin
BK
Pennington
Stephen R
SR
D000418:Albumins / Q000032:analysis*; D014408:Biomarkers, Tumor; D001798:Blood Proteins / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins / Q000097:blood*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D014466:Ultraviolet Rays
D000418:Albumins; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D020543:Proteome