Detection of bioradicals by in vivo L-band electron spin resonance spectrometry.
The applications of in vivo electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy have been impressive over a relatively short period despite the many obstacles which had to be overcome, such as dielectric absorption and biodestruction. The loop-gap resonators and modified loop coil systems have emerged as the most suitable resonators for in vivo EPR experiments. This paper briefly discusses instrumental aspects as a prelude to several examples related to the in vivo monitoring and detection of bioradicals. Recent progress in detection of bioradicals induced by drugs or chemicals is discussed with regard to nitrosocompounds, nitric oxide and metals in vivo. A clinical EPR application is also discussed.
Fujii
Hirotada
H
School of Health Sciences, Sapporo Medical University, Sapporo, Hokkaido 060-8556, Japan. sapmed.ac.jp
Berliner
Lawrence J
LJ
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001704:Biopolymers / Q000032:analysis / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000032:analysis / Q000378:metabolism*; D008872:Microwaves; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D009603:Nitroso Compounds / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*
D015415:Biomarkers; D001704:Biopolymers; D005609:Free Radicals; D008670:Metals; D009603:Nitroso Compounds; D009569:Nitric Oxide; D010100:Oxygen