Effect of treating sperm with low hypo-osmotic swelling test scores with chymotrypsin on pregnancy rates after conventional in vitro fertilization-embryo transfer.
The embryos formed from fertilizing oocytes with sperm with subnormal hypo-osmotic swelling tests with conventional insemination rather than intracytoplasmic sperm injection are very unlikely to implant. The toxic factor transferred to the zona pellucida by such sperm can be negated by pretreating the sperm with the protein digestive enzyme chymotrypsin, resulting in markedly improved implantation rates.
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Matthew L
ML
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Camden, NJ, USA.
Katsoff
Diane
D
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Jerome H
JH
Summers-Chase
Donna
D
D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D010865:Pilot Projects; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*; D016896:Treatment Outcome
D007038:Hypotonic Solutions