Structural analysis of arabinoxylans isolated from native and malted finger millet (Eleusine coracana, ragi).
Structural elucidation of purified arabinoxylans isolated from finger millet and its malt by methylation, GLC-MS, periodate oxidation, Smith degradation, NMR, IR, optical rotation, and oligosaccharide analysis indicated that the backbone was a 1,4-beta-D-xylan, with the majority of the residues substituted at C-3. The major oligosaccharide generated by endo xylanase treatment was homogeneous with a molecular weight of 1865 Da corresponding to 14 pentose residues as determined by MALDI-TOF-MS and gel filtration on Biogel P-2. The structural analysis of this oligosaccharide showed that it contained 8 xylose and 6 arabinose residues, substituted at C-3 (monosubstituted) and at both C-2 and C-3 (disubstituted).
Subba Rao
M V S S T
MV
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka, India.
Muralikrishna
G
G
D002240:Carbohydrate Sequence; D031748:Eleusine / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification
D014990:Xylans; C085118:arabinoxylan; C074948:(1-4)-xylan