[Construction and screening of the human phage antibody library from a patient with melanoma].
OBJECTIVE
To construct and screen the human phage antibody library from a patient with melanoma.


METHODS
Total RNA was extracted from the peripheral blood mononuclear cells (PBMCs) of a patient with melanoma who has survived for twenty years. The heavy chain Fd fragments and kappa light chains were amplified by RT-PCR. The amplified products were cloned into phagemid vector to construct Fab antibody library. The library was panned with two kinds of melanoma cells for 4 rounds.


RESULTS
Antibody library contained 1.2 x 10(8) different clones. Specific phage antibodies against one of the two melanoma cells were obtained after 4 rounds of panning.


CONCLUSIONS
Specific human phage antibodies against melanoma cells were obtained.
Yang
Jun
J
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. YangJun8@fmmu.edu.cn
Gao
Tian-wen
TW
Wang
Gang
G
Wang
Lei
L
Shen
Zhu
Z
Li
Chun-ying
CY
D000912:Antibodies, Neoplasm / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D019151:Peptide Library; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D019151:Peptide Library