Congenic mapping of type 1 diabetes--protective gene(s) in an interval of 4 Mb on rat chromosome 6q32.
Congenic BB.SHR rats introgressing a segment of SHR chromosome 6 onto BB/OK background showed a reduction of diabetes frequency by 72% compared with BB/OK. To identify underlying gene(s), the introgressed segment was shortened and the expression of seven genes (Yy1, Dlk1/Pref-1, Wd40 repeat, Cdc42, Rtl1, Traf3, and Tnfaip2) was studied in blood and spleen of non-diabetic BB/OK, BB.6S, and SHR males and females at an age of 30, 70, and 90 days. The phenotype of congenic sublines narrowed the diabetes-protective region to 4 Mb. The relative expression of Yy1 and Pref-1 in blood and of Pref-1 in spleen was significantly reduced by 50-90% in male and female BB.6S and SHR compared with BB/OK favouring Yy1 and Pref-1 as candidate genes. All other genes were differently expressed according to gender and strain.
Klöting
Nora
N
Department of Laboratory Animal Science, Medical Faculty, University of Greifswald, Karlsburg, Germany. kloeting@uni-greifswald.de
Klöting
Ingrid
I
D000818:Animals; D020296:Animals, Congenic; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000378:metabolism* / Q000517:prevention & control; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics* / Q000517:prevention & control; D008297:Male; D019655:Quantitative Trait, Heritable; D051381:Rats; D011913:Rats, Inbred BB / Q000235:genetics
D005819:Genetic Markers