title: Quantitative analysis of Fusarium mycotoxins in maize using accelerated solvent extraction before liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry.
abstract: A method for the simultaneous quantitative determination of deoxynivalenol (DON), fumonisin B1 (FB1) and zearalenone (ZEN) in maize by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCIMS/MS), using stable isotopically labelled and structural analogues internal standards, is described. The procedure involves accelerated solvent extraction followed by two solid-phase clean-up steps on strong anion exchange resin and a Mycosep column. Typical recoveries were calculated by spiking blank maize at three different concentrations for deoxynivalenol (200, 400 and 1000 microg kg(-1)) at 70%, for fumonisin B1 (100, 200 and 1000 microg kg(-1)) at 90%, and for zearalenone (50, 100 and 200 microg kg(-1)) at 40%. LC-APCIMS/MS analyses were realized in collision-induced dissociation on an ion-trap instrument to provide a high degree of selectivity and sensitivity. Extraction of ions from two transition reactions, monitored by LC-APCIMS/MS for each analyte, enabled a limit of detection for DON, FB1 and ZEN at, respectively, 10, 20 and 3 microg kg(-1), and a limit of quantification at, respectively, 50, 50 and 10 microg kg(-1). The robustness of the method was also evaluated with the analysis of wheat samples.
authors.affiliation: Nestlé Research Center, Nestec Ltd, Vers-Chez-Les-Blanc, CH-1000 Lausanne 26, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Royer
authors.forename: H-U
authors.initials: HU
authors.lastname: Humpf
authors.forename: P A
authors.initials: PA
authors.lastname: Guy
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002274:Carcinogens, Environmental / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis / Q000737:chemistry; D005670:Fusarium / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents; D014255:Trichothecenes / Q000032:analysis / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*; D015025:Zearalenone / Q000032:analysis / Q000737:chemistry
chemical_list: D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; D012997:Solvents; D014255:Trichothecenes; C056933:fumonisin B1; D015025:Zearalenone; C007262:deoxynivalenol