title: Sphedgehog is expressed by pigment cell precursors during early gastrulation in Strongylocentrotus purpuratus.
abstract: We have sequenced the Sphedgehog (Sphh) gene from the sea urchin Strongylocentrotus purpuratus. Sphh transcripts are detected first at the mesenchyme blastula stage, and they accumulate throughout early embryogenesis. The Sphh protein is produced by precursor pigment cells during early and midgastrulation. NiCl2 inhibits pigment cell differentiation in sea urchins. Here, we show that, in S. purpuratus, nickel affects a process(es) between 17 and 24 hr of development, corresponding to the time period when Sphh mRNA is first detected. However, nickel treatment does not alter the early expression of Sphh.
authors.affiliation: Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Egaña
authors.forename: Susan G
authors.initials: SG
authors.lastname: Ernst
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D004627:Embryonic Induction / Q000502:physiology*; D005775:Gastrula / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D009532:Nickel / Q000494:pharmacology; D047270:Strongylocentrotus purpuratus / Q000196:embryology* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D015534:Trans-Activators; C022838:nickel chloride; D009532:Nickel