Wheat pathogenesis-related proteins of class 4 have ribonuclease activity.
We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.
Caporale
Carlo
C
Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy.
Di Berardino
Iris
I
Leonardi
Luca
L
Bertini
Laura
L
Cascone
Annunziata
A
Buonocore
Vincenzo
V
Caruso
Carla
C
D000595:Amino Acid Sequence; D005670:Fusarium / Q000166:cytology / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism; D014908:Triticum / Q000201:enzymology* / Q000382:microbiology*
D010940:Plant Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; C062880:Win-1 proteins, plant; D012260:Ribonucleases