title: Wheat pathogenesis-related proteins of class 4 have ribonuclease activity.
abstract: We have demonstrated that wheatwin1, a wheat pathogenesis-related protein of class 4 (PR4), has ribonuclease activity. Both native and recombinant proteins hydrolyse RNA from wheat coleoptils and have antifungal activity. Sepharose-bound wheatwin1 is able to interact with either wheat or Fusarium culmorum RNA. 3D modelling studies showed that, like ribonucleases A and T1, the action mechanism should involve two His residues, an Arg residue and an Asp residue.
authors.affiliation: Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Caporale
authors.forename: Iris
authors.initials: I
authors.lastname: Di Berardino
authors.forename: Luca
authors.initials: L
authors.lastname: Leonardi
authors.forename: Laura
authors.initials: L
authors.lastname: Bertini
authors.forename: Annunziata
authors.initials: A
authors.lastname: Cascone
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Buonocore
authors.forename: Carla
authors.initials: C
authors.lastname: Caruso
mesh_terms: D000595:Amino Acid Sequence; D005670:Fusarium / Q000166:cytology / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000378:metabolism; D014908:Triticum / Q000201:enzymology* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; C062880:Win-1 proteins, plant; D012260:Ribonucleases