title: Structures of new beta-carboline-type alkaloids with antiallergic effects from Stellaria dichotoma(1,2).
abstract: The aqueous ethanolic extract from the roots of Stellaria dichotoma showed an antiallergic effect on ear passive cutaneous anaphylaxis (PCA) reaction in mice (in vivo) and inhibitory activity on the release of beta-hexosaminidase in RBL-2H3 cells (in vitro). From the aqueous ethanolic extract, new beta-carboline-type alkaloids, dichotomines A (1), B (2), C (3), and D (4) and dichotomides I (5) and II (6), were isolated. The structures of the new constituents (1-6) were determined on the basis of chemical and physicochemical evidence, including the application of the modified Mosher's method. The effects of the isolated constituents on the release of beta-hexosaminidase in RBL-2H3 cells were examined, and 3 was found to show inhibitory activity (IC(50) = 62 microM). Moreover, 3 also inhibited the releases of antigen-IgE-mediated TNF-alpha and IL-4 (19 and 15 microM, respectively) in RBL-2H3 cells, both of which participate in the late phase of type I allergic reactions.
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan.
authors.forename: Bohang
authors.initials: B
authors.lastname: Sun
authors.forename: Toshio
authors.initials: T
authors.lastname: Morikawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Supinya
authors.initials: S
authors.lastname: Tewtrakul
authors.forename: Li Jun
authors.initials: LJ
authors.lastname: Wu
authors.forename: Shoichi
authors.initials: S
authors.lastname: Harima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002243:Carbolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002681:China; D006596:Hexosaminidases / Q000037:antagonists & inhibitors; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010323:Passive Cutaneous Anaphylaxis; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029752:Stellaria / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
chemical_list: D000470:Alkaloids; D018926:Anti-Allergic Agents; D002243:Carbolines; D014409:Tumor Necrosis Factor-alpha; D006596:Hexosaminidases