Efficiency of different extraction solvent mixtures used in analyses of aflatoxins from a certified peanut meal reference material.
Four analytical methods with different extraction solvent mixtures were used to compare the efficiency of the extraction step in the analysis of aflatoxins from a reference material with a certified value of 206 +/- 13 microg aflatoxin B(1) kg(-1). The extraction mixtures used were chloroform-water (100 + 10), acetonitrile-water (60 + 40), acetone-water (85 + 15) and methanol-water (80 + 20). The analytical values determined from the extraction mixtures after correction for recovery were 211, 204, 163 and 120 microg kg(-1), respectively. The small amounts of aflatoxin B(2) in the reference material followed the same pattern. The selection of extraction solvent mixture is very important for achieving the true value and that correction for recovery does not always fully compensate for getting the correct analytical value.
Möller
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TE
National Food Administration, Uppsala, Sweden.
Nyberg
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M
D000096:Acetone; D000097:Acetonitriles; D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis / Q000302:isolation & purification*; D010367:Arachis / Q000737:chemistry*; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D000432:Methanol; D012997:Solvents
D000097:Acetonitriles; D000348:Aflatoxins; D012997:Solvents; D000096:Acetone; C029753:aflatoxin B2; D002725:Chloroform; D016604:Aflatoxin B1; D000432:Methanol; C032159:acetonitrile