Characterization of human anti-hrB-like monoclonal antibody.
To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RZRZ, Dc-, D--, Rhnull, and e+ hrB-. The supernatant is useful as a reagent to screen for hrB- blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.
Blancher
A P
AP
Laboratoire d'Immunologie, Pavillion Charles Lefebvre, CHU Purpan, 31059, Toulouse Cedex, France.
Reid
M E
ME
Alié-Daram
S J
SJ
Dugoujon
J M
JM
Roubinet
F L
FL