[Expression and significance of Hsp70 and PCNA during the development of forestomach carcinoma in NIH mice].
OBJECTIVE
To explore the expressions and significance of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the development of forestomach carcinoma in NIH mice induced with N-Nitrososarcosineethylester(NSEE).


METHODS
144 mice were divided into 6 groups and their stomachs were infused with NSEE. Then 24 mice were killed every two weeks and their forestomachs were used for the study of the expressions of Hsp70 and PCNA by immunohistochemical staining.


RESULTS
With the progress of carcinogenesis, the expression pattern of Hsp70 was up-down-up, but the expression trend was still increasing. On day 56 and day 70 after NSEE treatment, the expression level of Hsp70 was higher than that of control (P<0.05). On day 84, the expression level of Hsp70 was markedly higher than that of control (P<0.01). The expression of PCNA increased gradually with the carcinogenesis. As compared with the control, stronger expression was found on day 42 and day 56 (P<0.05) and notably stronger expression on day 84 (P<0.01). The expression of Hsp70 was positively correlated to that of PCNA (r=0.98, P<0.01).


CONCLUSIONS
In the development of forestomach carcinoma of NIH mice, the expressions of both Hsp70 and PCNA increased and were positively correlated with each other.
Li
Chun-mei
CM
Department of Basic Medical Sciences, School of Pharmacy, Yantai University, Yantai 264005, China.
Liu
Zhi-feng
ZF
Zhang
Hong-xu
HX
Li
Xiang
X
Gao
Yong-lin
YL
Liu
Ke
K
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D009602:Nitrosamines; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D009602:Nitrosamines; D018809:Proliferating Cell Nuclear Antigen; C028099:N-nitrososarcosine ethyl ester