[Isolation of genomic DNA from Cinnamomum cassia Presl].
OBJECTIVE
To extract high-quality DNA for RAPD and other molecular biology research from Chinese traditional medicine Cinnamomum cassia Presl..


METHODS
Improved CTAB method, low pH medium with high salt method (LPHS) and urea method were used for DNA extract. The yield and quality of DNA was analysed by electrophoresis, ratios of A260/A280 and PCR amplification.


RESULTS
The modified CTAB method and low pH medium with high salt method can produce high qualiy DNA for PCR amplification.
Xu
Hong
H
School of Life Science, Xiamen University, 361005.
Zhang
Jun
J
Zheng
Min
M
Yuan
Wenjie
W
D002593:Cetrimonium Compounds / Q000737:chemistry; D032904:Cinnamomum aromaticum / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D018745:Genome, Plant; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014508:Urea / Q000737:chemistry
D002593:Cetrimonium Compounds; D018744:DNA, Plant; D014508:Urea