Effect of tension on nerve regeneration in rat sciatic nerve transection model.
Excessive tension across a nerve repair is known to impair nerve regeneration. However, it is uncertain whether nerve grafting is necessary when end-to-end repair would result in only mild to moderate tension. This study investigated the effect of tension on nerve regeneration. Sciatic nerves of 48 Lewis rats were transected and then repaired primarily after resection of 0-, 3-, 6-, or 9-mm lengths of nerve. Postoperative tension levels were quantified using a tensometer. Robust nerve regeneration was observed at 4 weeks in all except the 9-mm repair group, which showed lower nerve fiber counts, percent neural tissue, and nerve density (P < 0.05) and decreased functional recovery. These data indicate that modest levels of tension are well tolerated, but nerve regeneration drops precipitously once a critical tension threshold is exceeded. This threshold was between 0.39 and 0.56 N in the model studied, corresponding to a nerve defect between 6 mm and 9 mm.
Sunderland
Ian R P
IR
Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA.
Brenner
Michael J
MJ
Singham
Janakie
J
Rickman
Susan R
SR
Hunter
Daniel A
DA
Mackinnon
Susan E
SE
D000818:Animals; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology* / Q000601:surgery*