[Inhibition of hepatitis B virus replication by RNA interference in vitro].
OBJECTIVE
To design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs (siRNA) that targets HBV core gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.


METHODS
HepG2 2.2.15 was used as target cells. The plasmid and liposome metafectene were cotransfected into the cultured cells, HBV DNA were analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR.


RESULTS
The plasmid expressing siRNA was successfully constructed. The two constructed siRNAs could effectively inhibit HBV replication, and their inhibitive effect on HBV was dose-dependent.


CONCLUSIONS
These results showed that siRNA could substantially inhibit HBV replication in the infected cells
Zhu
Cai
C
Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Fan
Xue-Gong
XG
Li
Ning
N
Ying
Ruo-Su
RS
D006515:Hepatitis B virus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology; D034622:RNA Interference; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics*