title: B-cell chronic lymphocytic leukemia with aberrant CD8 expression: genetic and immunophenotypic analysis of prognostic factors.
abstract: B-cell chronic lymphocytic leukemia (B-CLL) is phenotypically characterized by cell surface co-expression of CD19, CD20, CD5, and CD23. However, the concomitant presence of other antigens distinctive of a particular leukocyte subset, e.g. T cells, is an unusual finding in B-CLL. In the present report, a case of B-CLL with aberrant expression of the T-cell-associated antigen CD8 is described. Flow cytometric analysis of the patient's cells demonstrated lack of CD38 expression, and cytogenetic analysis by FISH did not show any abnormalities of chromosomes 17p, 11q, and 12. Furthermore, the B-CLL cells expressed only low levels of the tyrosine kinase ZAP-70, which was in agreement with the mutated status of the immunoglobulin heavy-chain variable-region gene (IgVH). In summary, the determined profile of prognostic markers together with the highly stable and indolent disease in the described patient suggest a good prognosis of B-CLL with aberrant CD8 expression.
authors.affiliation: Department of Hematology and Oncology, Georg-August-University Goettingen, Germany. R.Schroers@medizin.uni-goettingen.de
authors.forename: Roland
authors.initials: R
authors.lastname: Schroers
authors.forename: Tobias
authors.initials: T
authors.lastname: Pukrop
authors.forename: Jan
authors.initials: J
authors.lastname: Dürig
authors.forename: Detlef
authors.initials: D
authors.lastname: Haase
authors.forename: Ulrich
authors.initials: U
authors.lastname: Dührsen
authors.forename: Lorenz
authors.initials: L
authors.lastname: Trümper
authors.forename: Frank
authors.initials: F
authors.lastname: Griesinger
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism*; D002869:Chromosome Aberrations; D005434:Flow Cytometry; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000235:genetics; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D014408:Biomarkers, Tumor; D016827:CD8 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human