title: Modulation of osteoblast activity by serum from diabetic and non-diabetic patients on hemodialysis: a three-dimensional culture study.
abstract: BACKGROUND
  The aims of our study were to develop a three-dimensional model based on normal human osteoblasts and to determine, with this method, the osteoblastic response to sera of non-diabetic and diabetic patients on hemodialysis for chronic renal failure.
  
  
  METHODS
  A protocol for culture of osteoblastic cells in three-dimensional meshworks of bovine type I collagen was developed. The effect on cultures of sera from three groups of patients was studied: 1) 12 diabetic patients on hemodialysis [age: 65 +/- 5.2 years; sex: 6 men, 6 women; duration of dialysis treatment: 21.25 (641) months]; 2) 12 non-diabetic patients on hemodialysis [age: 65.5 +/- 5.4 years; sex: 6 men, 6 women; duration of dialysis treatment: 21.35 (6-40) months]; and 3) 12 healthy volunteer donors (age: 64.2 +/- 4.9 years; 6 men, 6 women). The three-dimensional cultures obtained were processed in the same way as undecalcified bone biopsies, sectioned and stained. A histomorphometrical study was conducted. Parameters calculated were cell number, volume of newly-formed collagen, volume of newly-formed collagen per cell, mineral volume and mineral volume per cell.
  
  
  RESULTS
  Number of osteoblastic cells was significantly higher in non-diabetic than in diabetic and control serum-treated cultures. Newly-formed collagen volume was significantly higher in non-diabetic serum-treated cultures than in controls. Deposited mineral volume was significantly lower in diabetic and non-diabetic serum-treated cultures compared to control serum-treated cultures.
  
  
  CONCLUSIONS
  The model of three-dimensional culture proposed in the present study is useful in the study of different osteoblast functions including the rate of collagen formation and mineralization.
authors.affiliation: Department of Pathology, Mar Hospital, Autònoma University, Barcelona, Spain. 18583@imas.imim.es
authors.forename: Sergi
authors.initials: S
authors.lastname: Serrano
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Mariñoso
authors.forename: Montserrat
authors.initials: M
authors.lastname: Nacher
authors.forename: Armando
authors.initials: A
authors.lastname: Torres
authors.forename: Xavier
authors.initials: X
authors.lastname: Cuevas
authors.forename: Josep
authors.initials: J
authors.lastname: Loreta
authors.forename: Assumpta
authors.initials: A
authors.lastname: Munné
authors.forename: Adolfo
authors.initials: A
authors.lastname: Diez
mesh_terms: D000368:Aged; D015519:Bone Density; D002452:Cell Count; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D003928:Diabetic Nephropathies / Q000097:blood* / Q000628:therapy; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000097:blood; D006435:Renal Dialysis
chemical_list: D024042:Collagen Type I; D010281:Parathyroid Hormone