title: Expression of mucins and E-cadherin in gastric carcinoma and their clinical significance.
abstract: OBJECTIVE
  To investigate the expression of three types of mucin (MUC1, MUC2, MUC5AC) and E-cadherin in human gastric carcinomas and their clinical significance.
  
  
  METHODS
  Ninety-four gastric cancer specimens were classified according to WHO criteria and detected by immunohistochemical assay of expression of mucins and E-cadherin.
  
  
  RESULTS
  The positive expression rates of MUC1, MUC2, MUC5AC and E-cadherin were 82% (77/94), 84% (79/94), 40% (38/94) and 56% (53/94) respectively. MUC1 expression was significantly correlated with the types of cancer (the positive rates of MUC1 in well and moderately differentiated tubular adenocarcinoma, poorly differentiated adenocarcinoma, signet-ring cell carcinoma and mucinous carcinoma were 91%, 87%, 71%, 71%, respectively, P<0.05), age of patients (the positive rates of it among the people who are younger than 40 years, between 40-60 years and over 60 year were 74%, 81%, 89%, P<0.05), lymph nodes involvement (the positive rates in the non-interfered group and the interfered group were 78%, 85%, P<0.05) and tumor size (the positive rates in the tumors with the size less than 3 cm, 3-6 cm and larger than 6 cm were 69%, 92%, 69%, P<0.05); MUC2 expression was significantly associated with types of cancers and had the strongest expression in mucinous carcinomas(the positive rates of MUC2 in well and moderately differentiated tubular adenocarcinoma, poorly differentiated adenocarcinoma, signet-ring cell carcinoma and mucinous carcinoma were 94%, 70%, 81%, 100%, P<0.05), but it had no obvious relation to age, gender, tumor location, lymph nodes involvement, depth of invasion and metastasis to extra-gastric organs (P>0.05); MUC5AC expression was not related to any of the characteristics investigated except that it had relation to gender, whereas MUC5AC showed the tendency to higher expression in less invasive lesions and lower expression in advanced stage cancers (P>0.05); No significant difference was found for E-cadherin expression. There were strong positive relationships between the expression of MUC1 and E-cadherin, MUC2 and E-cadherin, MUC1 and MUC2 (R = 0.33, R = 0.22, R = 0.32, respectively, P<0.05). According to the COX proportional hazards model, older patients, involvement of lymph nodes, different types of gastric cancer and MUC2 expression were significantly associated with poorer outcome of gastric carcinoma patients (beta = 0.08, beta = 3.94, beta = 1.33, beta = 0.75, respectively, P<0.05).
  
  
  CONCLUSIONS
  MUC1 and MUC2 are good markers of different types of gastric cancer. MUC2 is especially a good marker of mucinous carcinoma. MUC1, MUC2 may interfere with the function of E-cadherin in gastric carcinomas, and have synergic effect on progression of gastric cancers.
authors.affiliation: Department of Pathology, Fuxing Hospital, Capital University of Medical Sciences, Beijing 100038, China.
authors.forename: Hong-Kai
authors.initials: HK
authors.lastname: Zhang
authors.forename: Qiu-Min
authors.initials: QM
authors.lastname: Zhang
authors.forename: Tie-Hua
authors.initials: TH
authors.lastname: Zhao
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Yong-Fen
authors.initials: YF
authors.lastname: Yi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000601:surgery; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000378:metabolism*; D055262:Mucin-2; D009077:Mucins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C116055:MUC1 tandem repeat peptide; C524625:MUC2 protein, human; D018396:Mucin-1; D055262:Mucin-2; D009077:Mucins; D010446:Peptide Fragments