Optic nerve regeneration in polyglycolic acid-chitosan conduits coated with recombinant L1-Fc.
Autografts have been extensively studied to facilitate optic nerve (ON) regeneration in animal experiments, but the clinical application of this approach to aid autoregeneration has not yet been attempted. This study aims to explore the guided regeneration by an artificial polyglycolic acid-chitosan conduit coated with recombinant L1-Fc. Consistent with previous studies; in vitro assay showed that both chitosan, a natural biomaterial, and the neural cell adhesion molecule L1-Fc enhanced neurite outgrowth. Rat optic nerve transection was used as an in vivo model. The implanted PGA-chitosan conduit was progressively degraded and absorbed, accompanied by significant axonal regeneration as revealed by immunohistochemistry, anterograde and retrograde tracing. The polyglycolic acid-chitosan conduit coated with L1-Fc showed more effective to promote axonal regeneration and remyelination. Taken together, our observations demonstrated that the L1-Fc coated PGA-chitosan conduits provided a compatible and supportive canal to guild the injured nerve regeneration and remyelination.
Xu
Gang
G
Department of Clinical Research, Singapore General Hospital, Block A, No. 7 Hospital Drive, Singapore 169608.
Nie
Du-yu
DY
Wang
Wen-zu
WZ
Zhang
Pei-hua
PH
Shen
Jie
J
Ang
Beng-ti
BT
Liu
Guo-hua
GH
Luo
Xue-gang
XG
Chen
Nan-liang
NL
Xiao
Zhi-cheng
ZC
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D045744:Cell Line, Tumor; D048271:Chitosan / Q000627:therapeutic use*; D005260:Female; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D039842:Neural Cell Adhesion Molecule L1 / Q000627:therapeutic use*; D009900:Optic Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D020221:Optic Nerve Injuries / Q000188:drug therapy* / Q000473:pathology; D011100:Polyglycolic Acid / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use
D001672:Biocompatible Materials; D039842:Neural Cell Adhesion Molecule L1; D011994:Recombinant Proteins; D011100:Polyglycolic Acid; D048271:Chitosan