Multiplexed sorting of libraries on libraries: a novel method for empirical protein design by affinity-driven phage enrichment on synthetic peptide arrays.
Chemically synthesized peptide arrays on planar cellulose carriers are proposed as libraries of ligands suitable for the multiplexed simultaneous capture of peptide-specific acceptor proteins from a large randomly mutagenized library of acceptor proteins presented on bacteriophage M13 particles. This experimental set-up can be exploited to rapidly screen for individual new, distinct binding partners from two complementary libraries (two-dimensional screening). The technical feasibility of this empirical protein design approach was demonstrated with calmodulin as an aceptor protein using an array of mastoparan variants for multiplexed phage affinity enrichment.
Hultschig
Claus
C
Research Group for Molecular Recognition, GBF (German Research Centre for Biotechnology), Braunschweig, Germany.
Frank
Ronald
R
D000595:Amino Acid Sequence; D017104:Bacteriophage M13; D001483:Base Sequence; D002147:Calmodulin / Q000138:chemical synthesis / Q000737:chemistry; D003001:Cloning, Molecular; D015195:Drug Design; D004926:Escherichia coli / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides; D016133:Polymerase Chain Reaction; D040081:Protein Array Analysis; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D020349:Surface Plasmon Resonance; D014862:Wasp Venoms / Q000737:chemistry
D002147:Calmodulin; D036341:Intercellular Signaling Peptides and Proteins; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D014862:Wasp Venoms; C037500:mastoparan