Diversity of oleic acid, ricinoleic acid and linoleic acid conversions among Pseudomonas aeruginosa strains.
Sixteen Pseudomonas aeruginosa strains, including patent strain NRRL B-18602, three recent isolates from composted materials amended with ricinoleic acid, and 12 randomly selected from the holdings of the ARS Culture Collection, were examined for their fatty acid converting abilities. The study examined the bioconversion of oleic acid to 7,10-dihydroxy-8( E)-octadecenoic acid (DOD) and ricinoleic acid to 7,10,12-trihydroxy-8( E)-octadecenoic acid (TOD). A new DOD-like compound from linoleic acid was observed. All strains except NRRL B-247 exhibited varying levels of DOD production. NRRL B-1000, NRRL B-18602 and NRRL B-23258 with yields up to 84% were among the best DOD producers. TOD production generally paralleled DOD production at a relatively lower yield of up to 15%. Strains NRRL B-1000 and NRRL B-23260 were the best TOD producers. A DOD-like product in low yields was obtained from linoleic acid. The fatty acid bioconversion capability was related neither to growth rate nor to variation in the greenish pigmentation of the strains. Production of significant quantities of DOD and TOD from oleic and ricinoleic acids, respectively, appeared to be a characteristic trait of P. aeruginosa strains. A number of highly effective strains for DOD production were identified.
Kuo
Tsung Min
TM
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA. kuotm@ncaur.usda.gov
Nakamura
Lawrence K
LK
D003470:Culture Media; D004783:Environmental Microbiology; D005285:Fermentation; D006801:Humans; D006880:Hydroxy Acids; D019787:Linoleic Acid / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*; D009829:Oleic Acids / Q000096:biosynthesis; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism*
C432089:7,10,12-trihydroxy-8-octadecenoic acid; C477043:7,10-dihydroxy-8-octadecenoic acid; D003470:Culture Media; D006880:Hydroxy Acids; D009829:Oleic Acids; D012277:Ricinoleic Acids; D019301:Oleic Acid; D019787:Linoleic Acid; C030521:ricinoleic acid