Sensing capabilities of colloidal gold modified with a self-assembled monolayer of a glucose-carrying polymer chain on a glass substrate.
A disulfide-carrying polymer with pendent glucose residues (poly(2-methacryloyloxyethyl D-glucopyranoside)) was obtained by using a benzyl N,N-diethyldithiocarbamoyl derivative which shows the abilities of initiation, chain transfer, and termination (iniferter). The disulfide-carrying polymer was accumulated on a colloidal Au-immobilized glass substrate, and the usefulness of the polymer as a sensing element of concanavalin A (Con A) was examined by using a UV-visible spectrophotometer with the help of surface plasmon resonance. The sensor showed a concentration-dependent specific binding of Con A with a detection limit of 1.9 nM, and furthermore, it had a very high stability at high ionic strength. The polymer-coated device examined here was not only useful as a simple biosensor chip but is also expected to expand our knowledge of interfacial phenomena by introducing various functional polymers on colloidal Au.
Morokoshi
Shinta
S
Department of Chemical and Biochemical Engineering, Toyama University, 930-8555 Japan.
Ohhori
Kazuhiko
K
Mizukami
Kazuya
K
Kitano
Hiromi
H
D000327:Adsorption; D003102:Colloids / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013499:Surface Properties
D003102:Colloids; D049408:Luminescent Agents; D011108:Polymers; D006046:Gold; D005947:Glucose