[Effect of Yiqi Huayu recipe on peanut agglutinin-binding molecules and Schwann's cells in rats after lumbar nerve root compression].
OBJECTIVE
To study the effect of Yiqi Huayu Recipe on peanut agglutinin-binding molecules (PNA-BMs) and Schwann's cells (Sc) of the neuromuscular junction after L5 nerve root compression in rats.


METHODS
The rats were given Yiqi Huayu Recipe at the 10th, 20th, 30th and 60th day after L5 nerve root compression. Using immunohistochemistry and confocal laser scanning techniques, we observed the distributions of PNA-BMs and S-100 in the soleus muscle. The overlap area of PNA-BMs and S-100 was measured with NIH image technique.


RESULTS
The extracellular matrix (ECM) recognized by PNA played an important role in guiding the sprouting and extending of the Schwann's cells. The agglomeration, sprouting and extension of the terminal Sc and the overlap area of Sc with PNA-BMs in the Yiqi Huayu Recipe group were better than those in the control group.


CONCLUSIONS
Yiqi Huayu Recipe can promote the growth of ECM and Schwann's cells and accelerate the nerve regeneration process.
Zhou
Chong-Jian
CJ
Institute of Spinal Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. zhouchongjian11@sina.com
Su
Jun-Ren
JR
Wang
Yong-Jun
YJ
Hou
Bao-Xing
BX
Liu
Mei
M
Shi
Qi
Q
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D008516:Medicine, Chinese Traditional; D018613:Microscopy, Confocal; D011843:Radiculopathy / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011975:Receptors, Mitogen / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism*; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism; D016896:Treatment Outcome
D004365:Drugs, Chinese Herbal; D011975:Receptors, Mitogen; D009418:S100 Proteins; C038223:peanut agglutinin receptor