Effects of transforming growth factor-beta 1 on the early stages of healing of the Achilles tendon in a rat model.
We studied the effects of transforming growth factor-beta 1 (TGF-beta 1) on the genetic expression of procollagen type I and III and its effects on structural properties in the early stages of healing in rat Achilles tendon. The Achilles tendons in 90 rats were transsected and repaired immediately. TGF-beta 1 dissolved in phosphate-buffered saline was injected locally at the repair site using two different doses, and outcomes in both groups were compared to that in the control group given phosphate-buffered saline only. Five animals in each group were killed at one, two, and four weeks postoperatively, and the healing tendon was evaluated. A dose-dependent increase in the expression of procollagen type I and III mRNA was found one week postoperatively. The failure load and stiffness of the healing tendon were increased by TGF-beta 1 at two and four weeks.
Kashiwagi
Kenji
K
Department of Orthopaedic Surgery, Division of Clinical Medical Science, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. kkashiwagi@chugokuh.rofuku.go.jp
Mochizuki
Yu
Y
Yasunaga
Yuji
Y
Ishida
Osamu
O
Deie
Masataka
M
Ochi
Mitsuo
M
D000125:Achilles Tendon / Q000293:injuries* / Q000503:physiopathology; D000818:Animals; D001696:Biomechanical Phenomena; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006133:Growth Substances / Q000008:administration & dosage*; D007267:Injections; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D013708:Tendon Injuries / Q000188:drug therapy* / Q000503:physiopathology; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*; D014945:Wound Healing / Q000187:drug effects*
D024042:Collagen Type I; D024061:Collagen Type III; D006133:Growth Substances; D016212:Transforming Growth Factor beta