Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis.
A recombinant antigen-based single serum dilution enzyme-linked immunosorbent assay (ELISA) was developed to measure the specific antibody activity in sera of dogs with leptospirosis. The recombinant antigen developed and used in the assay was specific for the pathogenic serovars of Leptospira. A linear relationship was found to exist between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial-dilution method. Regression analysis was used to determine a standard curve from which an equation can be derived that allows demonstration of the mentioned correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay was proved to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT).
Dey
Sohini
S
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India. drsohinid@ivri.up.nic.in
Mohan
C Madhan
CM
Kumar
T M A Senthil
TM
Ramadass
P
P
Nainar
A Mahalinga
AM
Nachimuthu
K
K
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001483:Base Sequence; D004283:Dog Diseases / Q000175:diagnosis / Q000382:microbiology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007919:Leptospira / Q000276:immunology / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008074:Lipoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins