Oxidative stress and free radical damage in patients with acute dipterex poisoning.
OBJECTIVE
To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP may cause oxidative stress and free radical damage.


METHODS
Fifty ADPPs and fifty healthy adult volunteers (HAVs) whose ages, gender and others were matched with the ADPPs were enrolled in a randomized controlled study, in which concentrations of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as concentration of lipoperoxide (LPO), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometric analytical methods.


RESULTS
Compared with the average values of experimental parameters in the HAVs group, the average values of plasma NO and erythrocyte LPO in the ADPPs group were significantly increased (P<0.0001), while those of plasma VC, VE and beta-CAR as well as erythrocyte SOD, CAT, GPX and AChE in the ADPPs group were significantly decreased (P<0.0001). Bivariate correlation analysis and partial correlation analysis suggested that when NO and LPO values were increased, and VC, VE, beta-CAR, SOD, CAT and GPX values were decreased in the ADPPs, AChE value was decreased gradually in the ADPPs (P<0.001-0.0001). Reliability analysis of experimental parameters reflecting oxidative stress and free radical damage in the ADPPs showed that the reliability coefficient (8 items) alpha=0.6909, and the standardized item alpha=0.8574.


CONCLUSIONS
The findings in the present study suggest that ADP can cause oxidative stress and free radical damage, and inhibit markedly erythrocyte acetylcholinesterase activity in ADPPs.
Zhou
Jun-Fu
JF
Second Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang, China. jfzhou@zju.edu.cn
Zhou
Wen
W
Zhang
Shu-Mei
SM
Luo
Yan-Er
YE
Chen
Huai-Hong
HH
D000110:Acetylcholinesterase / Q000097:blood; D000293:Adolescent; D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D002681:China; D002800:Cholinesterase Inhibitors / Q000506:poisoning*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007306:Insecticides / Q000506:poisoning*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D011041:Poisoning / Q000097:blood; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood; D014236:Trichlorfon / Q000506:poisoning*; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene / Q000097:blood
D002800:Cholinesterase Inhibitors; D005609:Free Radicals; D007306:Insecticides; D008054:Lipid Peroxides; D019207:beta Carotene; D014810:Vitamin E; D009569:Nitric Oxide; D014236:Trichlorfon; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D001205:Ascorbic Acid