[Evaluation of proinflammatory cytokine (TNF-alpha, IL-1beta, IL-6, IFN-gamma) concentrations in serum and cerebrospinal fluid of patients with neuroborreliosis].
OBJECTIVE
Neuroborreliosis is a tick transmitted disease which becomes an increasingly frequent diagnostic and therapeutic problem in physician practice. The purpose of this work was to evaluate the concentration of proinflammatory cytokines: IL-1beta, IL-6, TNF-alpha, IFN-gamma in serum and cerebrospinal fluid of patients with neuroborreliosis.


METHODS
20 persons with diagnosed neuroborreliosis and 10 persons as a control group were examined in this study. The examination of serum and cerebrospinal fluid was performed twice, before and after 4-week therapy with antibiotics. The concentration of cytokines was measured by the ELISA method using kits of Bender Medical System and Quantikine RD Systems.


RESULTS
The concentration of measured cytokines IL-1beta, IL-6, TNF-alpha and IFN-gamma in serum and cerebrospinal fluid was significantly higher before therapy. After 4-week therapy with antibiotics the concentration of cytokines in cerebrospinal fluid decreased but was still higher than in the control group except for IL-1beta.


CONCLUSIONS
The detection of proinflammatory cytokine concentration in serum and cerebrospinal fluid might be helpful as another parameter monitoring the inflammation course and therapy efficacy.
Kondrusik
Maciej
M
Klinika Chorób Zakaźnych i Neuroinfekcji, Akademia Medyczna w Białymstoku. mkondrusik@poczta.onet.pl
Swierzbińska
Renata
R
Pancewicz
Sławomir
S
Zajkowska
Joanna
J
Grygorczuk
Sambor
S
Hermanowska-Szpakowicz
Teresa
T
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood* / Q000134:cerebrospinal fluid; D007375:Interleukin-1 / Q000097:blood* / Q000134:cerebrospinal fluid; D015850:Interleukin-6 / Q000097:blood* / Q000134:cerebrospinal fluid; D020852:Lyme Neuroborreliosis / Q000097:blood* / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid / Q000378:metabolism*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma
Ocena stezeń wybranych cytokin prozapalnych: TNF-alpha, IL-1beta, IL-6 oraz IFN-gamma w płynie mózgowo-rdzeniowym i surowicy u chorych na neuroborelioze.