Dissemination of Escherichia coli producing AmpC-type beta-lactamase (CMY-11) in Korea.
Among the 51 clinical isolates collected from a university hospital in Korea, nine isolates were resistant to cephamycins. Nine isolates were shown to produce CMY-11 and these also included three isolates producing TEM-1. The results from ERIC-PCR revealed that dissemination of CMY-11 was due to outbreaks of resistant species and to the intra-species spread of resistance to cephamycins in Korea. CMY-11 beta-lactamase genes from nine clinical isolates that were responsible for resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin and amoxicillin-clavulanic acid, were cloned and characterised. A sequence identical to the common regions in In6, In7 and a novel integron from pSAL-1 was found upstream from bla(CMY-11) gene at nucleotide 1-71. Eighteen nucleotides between position 71 and 72 were inserted into the bla(CMY-11) gene.
Kim
Jae Young
JY
Bio Technology Innovation Centre, Youngdong University, Chungbuk 370-701, Republic of Korea.
Song
Jae Seok
JS
Bak
Song Hee
SH
Cho
Young Eun
YE
Kim
Dae Wi
DW
Jeong
Seok Hoon
SH
Park
Yeong-Min
YM
Lee
Kye Joon
KJ
Lee
Sang Hee
SH
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D007723:Korea; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; C048795:AmpC beta-lactamases; D001618:beta-Lactamases