Propylene glycol releases calcium from mitochondrial stores in rat cerebrocortical synaptosomes.
In Ca2+-free medium, propylene glycol (PG) increases the cytosolic-free Ca2+ concentration ([Ca2+]i) in rat cerebrocortical synaptosomes. In the current studies, the authors investigated the intracellular source of this Ca2+. PG (0.5-5% v/v) dose-dependently increased [Ca2+]i in Ca2+-free medium. The increase in [Ca2+]i was completely inhibited by pretreatment with the mitochondrial uncoupler, carbonylcyanide m-chlorophenylhydrazone (CCCP, 10 microM). The inhibitory effect of CCCP was dependent on the concentration and the pretreatment time. These results suggest that, in Ca2+-free medium, PG increases [Ca2+]i in rat cerebrocortical synaptosomes by releasing Ca2+ from mitochondrial stores.
Satoh
Eiki
E
Department of Pathobiological Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. es@obihiro.ac.jp
Murakami
Kei
K
Nishimura
Masakazu
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002540:Cerebral Cortex; D005260:Female; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D019946:Propylene Glycol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
D012997:Solvents; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D019946:Propylene Glycol; D002118:Calcium