title: [Purification of recombinant humanized anti-HBsAg Fab antibody by affinity chromatography].
abstract: OBJECTIVE
  To establish a steady purification method for producing recombinant humanized anti-HBsAg Fab from yeast fermentation supernatant.
  
  
  METHODS
  Humanized anti-HBs scFv was used to immunize BALB/c mice to obtain anti-scFv monoclonal antibody (mAb) which was used to purify recombinant humanized anti-HBsAg Fab from yeast fermentation supernatant by affinity chromatography.
  
  
  RESULTS
  The purity of purified recombinant anti-HBsAg Fab was above 95% and its recovery rate was about 75%-85%.
  
  
  CONCLUSIONS
  An efficient affinity chromatography suitable for purification of recombinant humanized anti-HBsAg Fab in large-scale was established.
authors.affiliation: Lab. of Molecular Immunology and Antibody Engineering, Life Sciences and Technology College, Jinan University, Guangzhou 510632, China. tdengn@jnu.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Deng
authors.forename: Wen-yin
authors.initials: WY
authors.lastname: Chen
authors.forename: Jun-jian
authors.initials: JJ
authors.lastname: Xiang
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Rao
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000915:Antibody Affinity; D002846:Chromatography, Affinity; D005285:Fermentation; D006510:Hepatitis B Antibodies / Q000276:immunology / Q000302:isolation & purification*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D007114:Immunization; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000302:isolation & purification*; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000302:isolation & purification*; D007135:Immunoglobulin Variable Region / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; C055966:immunoglobulin Fv