Sequence characterization, polymorphism and chromosomal localizations of the porcine PSME1 and PSME2 genes.
The full-length cDNA of porcine genes (PSME1 and PSME2) encoding proteasome activators PA28alpha- and beta-subunits were obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with their mammalian counterparts. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that porcine PSME1 and PSME2 genes are expressed in all eight tissues studied (liver, spleen, bladder, small intestine, kidney, heart, skeletal muscle and lung). The full-length genomic DNA of the porcine PSME1 and PSME2 genes were amplified by PCR. These two genes shared the same structure and were similar in size. A C/T single nucleotide polymorphism in PSME1 intron 8 detected as an SphI PCR-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency differences between Meishan, Tibetan, Large White, Qingping, and Duroc pigs. The association analysis using two experimental GY selection lines selected for growth rate or leanness suggested that the PSME1 genotype was associated with weaning weight. Analyses of somatic cell hybrid (SCHP) and radiation hybrid (IMpRH) panels revealed that both genes map to SSC7q15.3-q21 and closely linked to the T-cell receptor alpha (TCRA) gene.
Wang
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YF
Lab of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yu
M
M
te Pas
M F W
MF
Yerle
M
M
Liu
B
B
Fan
B
B
Xiong
T A
TA
Li
K
K
D000818:Animals; D001483:Base Sequence; D001835:Body Weight; D017931:DNA Primers; D015870:Gene Expression; D005787:Gene Frequency; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D021101:Radiation Hybrid Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics*
D017931:DNA Primers