A novel ATX-S10(Na) photodynamic therapy for human skin tumors and benign hyperproliferative skin.
OBJECTIVE
Photodynamic therapy (PDT) is a promising treatment for various skin tumors and other skin diseases. We investigated the potential therapeutic effects of PDT using ATX-S10(Na) ointment and a diode laser in mouse skin models of experimental skin tumors as well as transplanted human samples of superficial skin tumors and lesional psoriatic skin.


METHODS
ATX-S10(Na) ointment (1% w/v) was introduced into tape-stripped mouse skin, transplanted squamous cell carcinoma (SCC) samples and human skin diseases after topical application, then PDT was performed.


RESULTS
ATX-S10(Na) ointment (1% w/v) was introduced effectively into tape-stripped mouse skin and transplanted SCC samples after topical application, but was not detected after 48 h, as assessed by fluorescence microscopy. PDT, using 1% ATX-S10(Na) ointment and diode laser (50 J/cm(2)), was found to decrease epidermal thickness in 12-0-tetradecanoylphorbol-13-acetate (TPA)-treated mouse skin by 6 days. PDT with 1% ATX-S10(Na) ointment and diode laser (150 J/cm(2)) was also effective for transplanted SCC, and tumors were eliminated by 6 weeks. PDT against Bowen disease, basal-cell carcinoma, and psoriasis xenografts onto SCID mice also showed marked suppression of tumor growth and cell proliferation, respectively.


CONCLUSIONS
Our results indicate that ATX-S10(Na)-PDT is an effective treatment for various skin tumors and psoriasis in experimental mouse models.
Takahashi
Hidetoshi
H
Department of Dermatology, Asahikawa Medical College, 2-1-1-1 Midorigaoka higashi, Asahikawa 078-8510, Japan. ht@asahikawa-med.ac.jp
Itoh
Yasuhiro
Y
Nakajima
Susumu
S
Sakata
Isao
I
Iizuka
Hajime
H
D000818:Animals; D001913:Bowen's Disease / Q000188:drug therapy; D002273:Carcinogens / Q000009:adverse effects; D002280:Basal Cell Carcinoma / Q000188:drug therapy; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D053685:Laser Therapy; D051379:Mice; D008812:Mice, Hairless; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009824:Ointments; D010778:Photochemotherapy; D011166:Porphyrins / Q000008:administration & dosage / Q000627:therapeutic use*; D011565:Psoriasis / Q000188:drug therapy; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects; D012871:Skin Diseases / Q000188:drug therapy*; D012878:Skin Neoplasms / Q000188:drug therapy*; D013755:Tetradecanoylphorbol Acetate / Q000009:adverse effects; D014183:Transplantation, Heterologous
C115550:ATX-S10; D002273:Carcinogens; D009824:Ointments; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; D013755:Tetradecanoylphorbol Acetate