Studies on the endogenous phospholipids of mammalian kidney and their in vitro hydrolysis by endogenous phospholipases: a thin layer chromatographic and densitometric study.
The phosphoglycerides profile of six species of mammalian kidney (guinea pig, pig, cat, dog, mouse and rat) and their in vitro response to the endogenous phospholipases were determined by TLC technology in conjunction with densitometric measurements. Changes in their phospholipids profile subsequent to in vitro incubation of whole tissue homogenate of these kidneys for 60 min, at pH 7.4, 38 degrees C, and prior to phospholipids extraction have shown that the deacylation of the endogenous cardiolipin (CL) is the most prevalent lipolytic event of all mammalian kidneys studied. Concurrent with the deacylation of CL, there was also formation of monolysocardiolipin (MLCL) and a reduction in CL level. To a much lesser extent, lyso alkenyl phosphatidyl ethanolamine (LPE) was also produced concomitant with a decrease of the endogenous alkenyl phosphatidyl ethanolamine (PE) level. The deacylation of PE plasmalogen to its lyso form confirms the action of endogenous PLA(2) releasing sn-2 fatty acids.
Helmy
Fatma M
FM
Department of Biological Sciences, Delaware State University, 1200 N. Dupont Highway, Dover, DE 19901, USA. fhelmy@desu.edu
Hassanein
Mohamed
M
Juracka
Amal
A
D000818:Animals; D002308:Cardiolipins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D002415:Cats; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry / Q000379:methods; D004285:Dogs; D006168:Guinea Pigs; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D008322:Mammals; D051379:Mice; D010715:Phosphatidylglycerols / Q000737:chemistry / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats
D002308:Cardiolipins; D010715:Phosphatidylglycerols; D010740:Phospholipases