A bicarbonate anion-dependent anti-'N' MoAb.
While studying the serologic characteristics of certain monoclonal blood group antibodies, we observed a hybridoma clone (5A-11E10) with anti-N-like serologic specificity that was dependent on the presence of the bicarbonate anion. The diluted cell culture supernatant preferentially agglutinated M-N+ RBCs by immediate spin. This supernatant also agglutinated M-N+ RBCs that had been treated with trypsin or sialidase (to remove N-reactivity), suggesting anti-'N' activity. Anti-'N' specificity was confirmed by the supernatant's non-reactivity with N+ RBCs treated with papain (to remove 'N' reactivity) or with ('N'-negative) M+N-U- RBCs. The requirement for bicarbonate in the MoAb's formulation was not a function of pH. Both sodium and ammonium bicarbonate supported agglutination, but neither sulfate nor carbonate was effective.
Iyer
Y S
YS
Institute of Immunohaematology, 13th floor, New Multistorey-Building, KEM hospital compound, Parel, Mumbai-400 012, India.
Vasantha
K
K
Joshi
S R
SR
Patwardhan
M
M
Pujari
V
V
Jadhav
S
S
Mohanty
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D001639:Bicarbonates / Q000494:pharmacology*; D003956:Dialysis; D006386:Hemagglutination Tests; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D001639:Bicarbonates; D008951:MNSs Blood-Group System