High-resolution comparative mapping of pig Chromosome 4, emphasizing the FAT1 region.
The first quantitative trait locus (QTL) in pigs, FAT1, was found on Chromosome 4 (SSC4) using a Wild Boar intercross. Further mapping has refined the FAT1 QTL to a region with conserved synteny to both human Chromosomes 1 and 8. To both improve the comparative map of the entire SSC4 and to define the specific human chromosome region with conserved synteny to FAT1, we have now mapped 103 loci to pig Chromosome 4 using a combination of radiation hybrid and linkage mapping. The physical data and linkage analysis results are in very good agreement. Comparative analysis revealed that gene order is very well conserved across SSC4 compared to both HSA1 and HSA8. The breakpoint in conserved synteny was refined to an area of about 23 cR on the q arm of SSC4 corresponding to a genetic distance of less than 0.5 cM. Localizations of the centromeres do not seem to have been conserved between the two species. No remnants of the HSA1 centromere were detected on the corresponding region on SSC4 and traces from the centromeric region of SSC4 cannot clearly be revealed on the homologous region on HSA8. This refined SSC4 map and the comparative analysis will be a great aid in the search for the genes underlying the FAT1 locus.
Moller
Maria
M
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, 75124, Uppsala, Sweden.
Berg
Frida
F
Riquet
Juliette
J
Pomp
Daniel
D
Archibald
Alan
A
Anderson
Susan
S
Feve
Katia
K
Zhang
Yuandan
Y
Rothschild
Max
M
Milan
Denis
D
Andersson
Leif
L
Tuggle
Christopher K
CK
D000818:Animals; D015820:Cadherins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003433:Crosses, Genetic; D020224:Expressed Sequence Tags; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*
D015820:Cadherins; C502403:FAT1 protein, human; D005819:Genetic Markers; D012313:RNA