Stanniocalcin-1 as a novel marker to detect minimal residual disease of human leukemia.
Stanniocalcin is a glycoprotein hormone that regulates the calcium level in fish. We found that mRNA of human stanniocalcin 1 (STC-1) is detectable in phytohemagglutinin-stimulated T cells and in most human leukemia cell lines, suggesting a role of STC-1 for cell proliferation. This finding prompts us to study the usefulness of STC-1 for monitoring acute leukemia. The levels of STC-1 transcripts increased in patients with acute leukemia at diagnosis and relapse, as judged by quantitative real-time RT-PCR. Levels of transcripts rapidly decreased to within the cut-off levels, when the blast numbers decreased with chemotherapy. Prolonged elevation of STC-1 levels after treatment was associated with a poor prognosis. All of 7 patients relapsed 1 to 4 months after they showed an elevated level of the transcripts in clinical remission. These results indicate that STC-1 is a novel marker for minimal residual disease of acute leukemia, and for an early diagnosis of relapse.
Tohmiya
Yasuo
Y
Department of Rheumatology and Hematology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Koide
Yoshio
Y
Fujimaki
Shinichi
S
Harigae
Hideo
H
Funato
Tadao
T
Kaku
Mitsuo
M
Ishii
Tomonori
T
Munakata
Yasuhiko
Y
Kameoka
Junichi
J
Sasaki
Takeshi
T
D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood*; D002118:Calcium / Q000145:classification; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000097:blood / Q000175:diagnosis*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000502:physiology; D014158:Transcription, Genetic
D014408:Biomarkers, Tumor; D017931:DNA Primers; D006023:Glycoproteins; D012333:RNA, Messenger; C051795:teleocalcin; D002118:Calcium