Glucagon-like peptide 1 excites hypocretin/orexin neurons by direct and indirect mechanisms: implications for viscera-mediated arousal.
Glucagon-like peptide 1 (GLP-1) is produced by neurons in the caudal brainstem that receive sensory information from the gut and project to several hypothalamic regions involved in arousal, interoceptive stress, and energy homeostasis. GLP-1 axons and receptors have been detected in the lateral hypothalamus, where hypocretin neurons are found. The electrophysiological actions of GLP-1 in the CNS have not been studied. Here, we explored the GLP-1 effects on GFP (green fluorescent protein)-expressing hypocretin neurons in mouse hypothalamic slices. GLP-1 receptor agonists depolarized hypocretin neurons and increased their spike frequency; the antagonist exendin (9-39) blocked this depolarization. Direct GLP-1 agonist actions on membrane potential were abolished by choline substitution for extracellular Na+, and dependent on intracellular GDP, suggesting that they were mediated by sodium-dependent conductances in a G-protein-dependent manner. In voltage clamp, the GLP-1 agonist Exn4 (exendin-4) induced an inward current that reversed near -28 mV and persisted in nominally Ca2+-free extracellular solution, consistent with a nonselective cationic conductance. GLP-1 decreased afterhyperpolarization currents. GLP-1 agonists enhanced the frequency of miniature and spontaneous EPSCs with no effect on their amplitude, suggesting presynaptic modulation of glutamate axons innervating hypocretin neurons. Paraventricular hypothalamic neurons were also directly excited by GLP-1 agonists. In contrast, GLP-1 agonists had no detectable effect on neurons that synthesize melanin-concentrating hormone (MCH). Together, our results show that GLP-1 agonists modulate the activity of hypocretin, but not MCH, neurons in the lateral hypothalamus, suggesting a role for GLP-1 in the excitation of the hypothalamic arousal system possibly initiated by activation by viscera sensory input.
Acuna-Goycolea
Claudio
C
Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
van den Pol
Anthony
A
D000200:Action Potentials / Q000187:drug effects; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D001143:Arousal / Q000502:physiology*; D002794:Choline / Q000494:pharmacology; D004064:Digestive System / Q000294:innervation; D004435:Eating / Q000502:physiology; D000077270:Exenatide; D017930:Genes, Reporter; D005934:Glucagon / Q000502:physiology*; D052216:Glucagon-Like Peptide 1; D000067757:Glucagon-Like Peptide-1 Receptor; D018698:Glutamic Acid / Q000502:physiology; D007028:Hypothalamic Hormones / Q000096:biosynthesis; D007031:Hypothalamus / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D008543:Melanins / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009479:Neuropeptides / Q000032:analysis*; D064446:Orexin Receptors; D000068797:Orexins; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010446:Peptide Fragments / Q000494:pharmacology / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D010907:Pituitary Hormones / Q000096:biosynthesis; D011498:Protein Precursors / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D018027:Receptors, Glucagon / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology; D018013:Receptors, Neuropeptide; D026941:Sodium Channel Blockers / Q000494:pharmacology; D017552:Solitary Nucleus / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D014688:Venoms / Q000494:pharmacology; D014781:Viscera / Q000294:innervation
C000593834:Glp1r protein, mouse; D000067757:Glucagon-Like Peptide-1 Receptor; D007028:Hypothalamic Hormones; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009479:Neuropeptides; D064446:Orexin Receptors; D000068797:Orexins; D010446:Peptide Fragments; D010455:Peptides; D010907:Pituitary Hormones; D011498:Protein Precursors; D043562:Receptors, G-Protein-Coupled; D018027:Receptors, Glucagon; D018013:Receptors, Neuropeptide; D026941:Sodium Channel Blockers; D014688:Venoms; D018698:Glutamic Acid; D013779:Tetrodotoxin; C083773:exendin (9-39); C019463:melanin-concentrating hormone; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D000077270:Exenatide; D002794:Choline