Library screening for D-amino-acid oxidase gene: application of real-time PCR.
Quantitative real-time PCR shows the quantity in addition to the presence of the target sequence. This property seemed very useful for library screening. Then, real-time PCR was employed to screen for lambda phages carrying D-amino-acid oxidase gene from mouse genomic library. Using stepwise dilution screening combined with real-time PCR, positive phages were isolated in a short time.
Konno
R
R
Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan. konno@dokkyomed.ac.jp
Niwa
A
A
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D005821:Genetic Techniques; D016678:Genome; D051379:Mice; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D000594:Amino Acid Oxidoreductases