title: Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon.
abstract: By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.
authors.affiliation: Stem Cell Research Institute, Dibit, H.S. Raffaele, Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Faedo
authors.forename: Jane C
authors.initials: JC
authors.lastname: Quinn
authors.forename: Patrick
authors.initials: P
authors.lastname: Stoney
authors.forename: Jason E
authors.initials: JE
authors.lastname: Long
authors.forename: Catherine
authors.initials: C
authors.lastname: Dye
authors.forename: Massimo
authors.initials: M
authors.lastname: Zollo
authors.forename: John L R
authors.initials: JL
authors.lastname: Rubenstein
authors.forename: David J
authors.initials: DJ
authors.lastname: Price
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bulfone
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D018076:DNA, Complementary / Q000378:metabolism; D047108:Embryonic Development; D005136:Eye Proteins; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013687:Telencephalon / Q000196:embryology*; D013997:Time Factors; D014157:Transcription Factors
chemical_list: D018076:DNA, Complementary; C088334:Distal-less homeobox proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; C000605733:Pax6 protein, rat; D012097:Repressor Proteins; D014157:Transcription Factors