title: Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways.
abstract: We evaluated whether bone marrow (BM) inflammatory cells have capacity to traffic into the airways following allergen exposure in a mouse model of allergen-induced airway inflammation. We also evaluated the effect of IL-5 overexpression on (i) the production of eosinophils in BM, (ii) the accumulation of eosinophils, neutrophils and lymphocytes in blood and airways and (iii) the changes in CD34+ cell numbers in BM, blood and airways. Bromodeoxyuridine (BrdU) was used to label cells produced during the exposure period. Furthermore, CD3 splenocytes were adoptively transferred to investigate the BM inflammatory response. Allergen exposure induced traffic of BM eosinophils, neutrophils and lymphocytes to the airways and increased the number of BrdU+ eosinophils, neutrophils, lymphocytes and CD34+ cells in BALf. IL-5 overexpression enhanced the eosinophilopoiesis and increased the presence of BrdU+ eosinophils and CD34+ cells in airways and enhanced the number of CD34+ cells in BM and blood after allergen exposure. Adoptive transfer of CD3 lymphocytes overexpressing IL-5 caused increased BM eosinophilia. In conclusion, allergen exposure induces traffic of not only newly produced eosinophils but also newly produced neutrophils and lymphocytes into the airways.
authors.affiliation: Lung Pharmacology Group, Department of Respiratory Medicine and Allergology, Institute of Internal Medicine, Göteborg University, Göteborg, Sweden. anna-karin.johansson@lungall.gu.se
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Johansson
authors.forename: Svetlana
authors.initials: S
authors.lastname: Sergejeva
authors.forename: Margareta
authors.initials: M
authors.lastname: Sjöstrand
authors.forename: James J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Jan
authors.initials: J
authors.lastname: Lötvall
mesh_terms: D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology*; D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000276:immunology*; D006651:Histocytochemistry; D015848:Interleukin-5 / Q000276:immunology; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology*
chemical_list: D000485:Allergens; D018952:Antigens, CD34; D015848:Interleukin-5; D004676:Myelin Basic Protein; D010047:Ovalbumin; D001973:Bromodeoxyuridine