title: [Effect of KyoT2 binding protein KBP1 on RBP-J-mediated transcriptional activity].
abstract: OBJECTIVE
  To investigate the physical interaction between KyoT2 and KyoT2-binding protein 1 (KBP1) and the effect of KBP1 on RBP-J-mediated transcriptional activity.
  
  
  METHODS
  GST-pull down, co-immunoprecipitation, mammalian cell two hybrid assay and reporter gene assay were used to examine the physical and functional interactions between KyoT2 and KBP1.
  
  
  RESULTS
  KBP1 and KyoT2 could interact with each other both in-vitro and in-vivo, and overexpression of KBP1 could antagonize the suppressive effect of RING1 on RBP-J.
  
  
  CONCLUSIONS
  KBP1 may indirectly modulate Notch signaling pathway by competing with RING1 for binding sites on KyoT2.
authors.affiliation: Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Qin
authors.forename: Hua
authors.initials: H
authors.lastname: Han
mesh_terms: D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000166:cytology*; D060588:LIM Domain Proteins; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D063150:Polycomb Repressive Complex 1; D051880:Receptors, Notch / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C101149:FHL1 protein, human; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; D051880:Receptors, Notch; D011994:Recombinant Proteins; D063150:Polycomb Repressive Complex 1; C080355:RING1 protein, human