title: Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives.
abstract: Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser-induced fluorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser-induced fluorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefficients were better than 0.995. The relative standard deviation (n = 5) of the migration times and peak heights were 0.56-0.76 and 2.21-7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32-2.20 nM). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9-107.9%.
authors.affiliation: Department of chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Xie
authors.initials: X
authors.lastname: Jianping
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jiyou
authors.forename: Liu
authors.initials: L
authors.lastname: Huanxiang
authors.forename: Liu
authors.initials: L
authors.lastname: Jiaqin
authors.forename: Tian
authors.initials: T
authors.lastname: Jianniao
authors.forename: Chen
authors.initials: C
authors.lastname: Xingguo
authors.forename: Hu
authors.initials: H
authors.lastname: Zhide
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D001888:Boric Acids; D020374:Chromatography, Micellar Electrokinetic Capillary; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004655:Emulsions; D005453:Fluorescence; D007834:Lasers; D016014:Linear Models; D012680:Sensitivity and Specificity
chemical_list: D000596:Amino Acids; D001888:Boric Acids; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D009327:4-Chloro-7-nitrobenzofurazan; C032688:boric acid