Improving specificity of DNA hybridization-based methods.
Methods based on DNA reassociation in solution with the subsequent PCR amplification of certain hybrid molecules, such as coincidence cloning and subtractive hybridization, all suffer from a common imperfection: cross-hybridization between various types of paralogous repetitive DNA fragments. Although the situation can be slightly improved by the addition of repeat-specific competitor DNA into the hybridization mixture, the cross-hybridization outcome is a significant number of background chimeric clones in resulting DNA libraries. In order to overcome this challenge, we developed a technique called mispaired DNA rejection (MDR), which utilizes a treatment of resulting reassociated DNA with mismatch-specific nucleases. We examined the MDR efficiency using cross-hybridization of complex, whole genomic mixtures derived from human and chimpanzee genomes, digested with frequent-cutter restriction enzyme. We show here that both single-stranded DNA-specific and mismatched double-stranded DNA-specific nucleases can be used for MDR separately or in combination, reducing the background level from 60 to 4% or lower. The technique presented here is of universal usefulness and can be applied to both cDNA and genomic DNA subtractions of very complex DNA mixtures. MDR is also useful for the genome-wide recovery of highly conserved DNA sequences, as we demonstrate by comparing human and pygmy marmoset genomes.
Chalaya
Tatyana
T
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.
Gogvadze
Elena
E
Buzdin
Anton
A
Kovalskaya
Elena
E
Sverdlov
Eugene D
ED
D000818:Animals; D020137:Base Pair Mismatch; D002144:Callithrix; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry; D003851:Deoxyribonucleases / Q000378:metabolism; D015723:Gene Library; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D019974:Pan paniscus; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D004247:DNA; D003851:Deoxyribonucleases