A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography.
A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed-phase Supelcosil LC-18 (250 x 4.6 mm, 5 microm) column. The mobile phase consisted of acetonitrile:water (40:60 v/v) at a fl ow rate of 0.8 mL/min. The UV-detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 0.125 to 12.5 microg/mL with a correlation coefficient from 0.999. The limit of detection and limit of quanti fi cation were 0.091 and 0.181 microg/mL, respectively.
Koll
Michael
M
Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Saudi Arabia.
Hoenen
Hubertus
H
Aboul-Enein
Hassan Y
HY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D004355:Drug Stability; D007502:Iron Chelating Agents / Q000032:analysis*; D010848:Picolinic Acids / Q000032:analysis* / Q000633:toxicity; D012044:Regression Analysis; D012680:Sensitivity and Specificity
D007502:Iron Chelating Agents; D010848:Picolinic Acids; C030614:picolinic acid