title: Acute exercise alters Galphai2 protein expressions through the ubiquitin-proteasome proteolysis pathway in rat adipocytes.
abstract: The effects of acute exercise on the protein expressions of heterotrimeric G protein alpha subunits were examined in rat adipocytes. Galphai2 protein expression was significantly reduced 0 and 3h after exercise but increased 24h after exercise, without alterations in Galphai2 mRNA expressions. The protein expressions of other alpha subunits, Galphas, Galphai1, and Galphai3, were not influenced. Both the 26S proteasome activity and polyubiquitination of Galphai2 protein were significantly increased 0 and 3h after exercise. Whereas, proteasome activity was decreased, and the polyubiquitination of Galphai2 protein was returned to the control level 24h after exercise. The reductions in Galphai2 protein expressions 0 and 3h after exercise were completely prevented by the injection either of a proteasome inhibitor or of a beta-adrenergic receptor blocker prior to exercise. Thus, acute exercise altered the expression of Galphai2 protein via mechanisms which involve the coupling of beta-adrenergic receptors to an agonist with subsequent ubiquitin-proteasome-dependent proteolysis.
authors.affiliation: Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Junetsu
authors.initials: J
authors.lastname: Ogasawara
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Nazibur
authors.initials: N
authors.lastname: Rahman
authors.forename: Takako
authors.initials: T
authors.lastname: Kizaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Hitomi
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Izawa
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D009097:Multienzyme Complexes; D010447:Peptide Hydrolases / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000379:methods*; D010807:Physical Endurance / Q000502:physiology*; D011433:Propranolol / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D011518:Proto-Oncogene Proteins; D025801:Ubiquitin; C067713:lactacystin; D011433:Propranolol; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C498017:Gnai2 protein, rat; D000111:Acetylcysteine