title: Organic solvent tolerance of halophilic alpha-amylase from a Haloarchaeon, Haloarcula sp. strain S-1.
abstract: A halophilic archaeon, Haloarcula sp. strain S-1, produced extracellular organic solvent-tolerant alpha-amylase. Molecular mass of the enzyme was estimated to be 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This amylase exhibited maximal activity at 50 degrees C in buffer containing 4.3 M NaCl, pH 7.0. Moreover, the enzyme was active and stable in various organic solvents (benzene, toluene, and chloroform, etc.). Activity was not detected at low ionic strengths, but it was detected in the presence of chloroform at low salt concentrations. On the other hand, no activity was detected in the presence of ethyl alcohol and acetone.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan. bioeng@mail.cc.eng.toyo.ac.jp
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Fukushima
authors.forename: Toru
authors.initials: T
authors.lastname: Mizuki
authors.forename: Akinobu
authors.initials: A
authors.lastname: Echigo
authors.forename: Akira
authors.initials: A
authors.lastname: Inoue
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
mesh_terms: D000096:Acetone / Q000737:chemistry; D000681:Amylases / Q000737:chemistry; D002725:Chloroform / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000737:chemistry; D004795:Enzyme Stability; D000431:Ethanol / Q000737:chemistry; D019613:Haloarcula / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D012997:Solvents / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D007477:Ions; D012997:Solvents; D000096:Acetone; D000431:Ethanol; D002725:Chloroform; D004492:Edetic Acid; D000681:Amylases; D000516:alpha-Amylases