The effect of cyclin D1 overexpression in human head and neck cancer cells.
Overexpression of cyclin D1 in head and neck cancer has been suggested to be a poor prognostic factor. To understand the role of cyclin D1 expression in head and neck cancer, we overexpressed cyclin D1 in TU182 (a cell line derived from pharyngeal cancer) using a retroviral vector. Stable transfectants were isolated by neomycin (G418) selection. Compared to the parental and control-vector transfected cells, the cyclin D1 transfected cells revealed a decrease of the G1/G0 population and resulted in continuous proliferation under low serum conditions. Proliferation assays revealed an increase in resistance to cisplatin in cyclin D1 overexpressing cells. These observation suggest that deregulation of cyclin D1 may reduce growth factor requirements and contribute to the resistance to some chemotherapeutic agents among head and neck cancer patients.
Nakashima
Torahiko
T
Department of Otorhinolaryngology, Kyushu University, 3-1-1 Maidashi, 812-8582 Fukuoka, Japan. nakatora@qent.med.kyushu-u.ac.jp
Kuratomi
Yuichiro
Y
Yasumatsu
Ryuji
R
Masuda
Muneyuki
M
Koike
Koji
K
Umezaki
Toshiro
T
Clayman
Gary L
GL
Nakagawa
Takashi
T
Komune
Shizuo
S
D000970:Antineoplastic Agents / Q000494:pharmacology; D015152:Blotting, Northern; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D019938:Cyclin D1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D011379:Prognosis; D014162:Transfection; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D019938:Cyclin D1; D002945:Cisplatin