[Osteogenesis characteristics of cultured rat mesenchymal stem cells under bone induction condition].
OBJECTIVE
To investigate the osteogenesis characteristics of cultured rat mesenchymal stem cells (MSCs) under bone induction condition.


METHODS
MSCs were isolated from adult rat by using density gradient separation method. The osteogenic inducers were compounds of Dexone, beta-glycerophosphate sodium and vitamin C.


RESULTS
The MSC attachment formed soon after the seeding and grew into colonies with the appearance of fibroblastic cells. The osteogenic inducer with low dose of Dexone could promote the osteogenic differentiation of MSC. In the group of osteogenic inducer with low dose of Dexone, the expression of alkaline phosphatase (ALP) was remarkably increased after one week's induction, and the number of positive cells was (15.1 +/- 2.6), significantly higher than that of the control group (12.0 +/- 3.5) (P < 0.01). The calcified deposits began to appear in the group of osteogenic inducer with low dose of Dexone after one week's induction and was increased remarkably after three weeks, and the number of calcified deposits was (9.0 +/- 1.7), significantly higher than that of the control group (2.0 +/- 1.8) (P < 0.01).


CONCLUSIONS
MSC can differentiate into osteogenesis by osteogenic induction and may be used to provide seed cells for bone tissue engineering.
Chen
Dong-Feng
DF
Department of Anatomy, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China. cdf27212@21cn.com
Li
Hui
H
Zhou
Jian-Hong
JH
Xie
Yao
Y
Li
Yi-Wei
YW
Du
Shao-Hui
SH
Zhang
Yi
Y
Huang
Jie
J
Xu
Min
M
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D011100:Polyglycolic Acid / Q000494:pharmacology; D051381:Rats; D023822:Tissue Engineering / Q000379:methods
D011100:Polyglycolic Acid; D000469:Alkaline Phosphatase