Involvement of IL-1 family proteins in p38 linked cellular senescence of mouse embryonic fibroblasts.
Senescence of mammalian cultured cells is essentially organized by a machinery of cell division and cellular stresses induced by various extracellular stimuli. Here, we show that in mouse embryonic fibroblasts (MEFs) culture in vitro, expression of an inflammatory cytokine, interleukin-1beta (IL-1beta) and its antagonist, IL-1 receptor antagonist (IL-1Ra) are induced by senescence. The kinetics of IL-1beta-expression was similar to that of p38 activation during MEFs culture. We also found a distinguishable accelerated senescence in cell growth in IL-1Ra deficient MEFs culture. Our results suggest that IL-1beta signaling pathway is involved in activation of p38 linked cellular senescence.
Uekawa
Natsuko
N
Laboratory of Experimental Animal Model Research, National institute for longevity sciences, National Center for Geriatrics and Gerontology, 36-3, Gengo, Morioka-Cho, Obu-city, Aichi 474-8522, Japan.
Nishikimi
Akihiko
A
Isobe
Ken-Ichi
K
Iwakura
Yoichiro
Y
Maruyama
Mitsuo
M
D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology*; D004789:Enzyme Activation; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005786:Gene Expression Regulation; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D048051:p38 Mitogen-Activated Protein Kinases