HPLC identification of oversulphated chondroitin as the major glycosaminoglycan of human tonsils.
The glycosaminoglycans of human nasopharyngeal and palatine tonsils, obtained after surgical dissection due to tonsillitis, were isolated and characterized by means of enzyme susceptibility and HPLC. Chondroitin/dermatan sulphate were the major glycosaminoglycans identified. A large proportion of this glycosaminoglycan was made up of oversulphated structures, namely DeltaDi-di(4,6)S, which were found mainly in invertebrate tissues and in mast cells.
Triantaphyllidou
I E
IE
Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras 265 00, Greece.
Mastronikolis
N S
NS
Papadas
Th A
TA
Vynios
D H
DH
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002809:Chondroitin Sulfates / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D014066:Palatine Tonsil / Q000737:chemistry*
D006025:Glycosaminoglycans; D002809:Chondroitin Sulfates