Minocycline protects PC12 cells from ischemic-like injury and inhibits 5-lipoxygenase activation.
Minocycline protects animals against cerebral ischemia by inhibiting inflammation. To determine whether minocycline protects PC12 cells from in vitro ischemic-like injury and affects pro-inflammatory 5-lipoxygenase activation, the cell viability and 5-lipoxygenase translocation to nuclear membrane were observed after oxygen-glucose deprivation (OGD). We found that OGD reduced cell viability, which was attenuated by minocycline and 5-lipoxygenase inhibitor caffeic acid. 5-Lipoxygenase protein was detected in PC12 cells by immunohistochemical and Western blot analyses. OGD induced 5-lipoxygenase translocation to nuclear membranes, which was abolished by minocycline and caffeic acid. Thus, minocycline can protect PC12 cells from in vitro ischemic-like injury, and this effect may be partly related to the inhibition of 5-lipoxygenase activation.
Song
Ying
Y
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310031, China.
Wei
Er-Qing
EQ
Zhang
Wei-Ping
WP
Zhang
Lei
L
Liu
Jian-Ren
JR
Chen
Zhong
Z
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D016859:Lipoxygenase Inhibitors; D008911:Minocycline / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats
D016859:Lipoxygenase Inhibitors; D001094:Arachidonate 5-Lipoxygenase; D008911:Minocycline; D010100:Oxygen