title: Frequency of -163 C>A and 63 C>G single nucleotide polymorphism of cytochrome P450 1A2 in two African populations.
abstract: Cytochrome P450 1A2 (CYP1A2) is an important member of the cytochrome P450 superfamily of enzymes because of its involvement in the metabolism of some carcinogens and therapeutically important drugs. As a result, factors affecting the activity of the enzyme are the focus of considerable research effort as they may have important pharmacological or toxicological implications. CYP1A2 has been shown to exhibit a genetic polymorphism with most of the data, however, coming from studies in Caucasian and Oriental populations. In this study therefore, we investigated the frequencies of two point mutations, -163C>A and 63C>G, in two Bantu African populations. A total of 214 healthy subjects were recruited from Zimbabwe (n=143) and Tanzania (n=71). The two single nucleotide polymorphisms were detected using polymerase chain reaction-restriction fragment length polymorphism analysis. The frequency of -163A was 57% (95% confidence interval (CI), 54%, 60%) and 49% (95% CI, 45%, 53%) among Zimbabweans and Tanzanians, respectively, but the difference between the two populations was not statistically significant (p=0.123). The base change 63 C>G was not found in any of the subjects from the two populations. We report here a high frequency of -163 C>A base change and an absence of the 63 C>G change in the two African populations.
authors.affiliation: Drug Metabolism Group, Department of Biochemistry, University of Zimbabwe, Harare, Zimbabwe. cdandara@curie.uct.ac.za
authors.forename: Collet
authors.initials: C
authors.lastname: Dandara
authors.forename: Patience T
authors.initials: PT
authors.lastname: Basvi
authors.forename: Tashinga E
authors.initials: TE
authors.lastname: Bapiro
authors.forename: Jane
authors.initials: J
authors.lastname: Sayi
authors.forename: Julia A
authors.initials: JA
authors.lastname: Hasler
mesh_terms: D000349:Africa; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D017354:Point Mutation; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D019388:Cytochrome P-450 CYP1A2