Analysis of polyphenolic antioxidants from the fruits of three pouteria species by selected ion monitoring liquid chromatography-mass spectrometry.
Pouteria campechiana, Pouteria sapota, and Pouteria viridis are tropical plants in the Sapotaceae family that bear edible fruits. The fresh fruits of these three Pouteria species were each extracted, and activity-guided fractionations were performed to identify the antioxidant constituents. Seven polyphenolic antioxidants, gallic acid (1), (+)-gallocatechin (2), (+)-catechin (3), (-)-epicatechin (4), dihydromyricetin (5), (+)-catechin-3-O-gallate (6), and myricitrin (7), were isolated and identified. Extracts of the three Pouteria fruits were analyzed by a selected ion monitoring liquid chromatography-mass spectrometry method to quantify their polyphenolic antioxidants. The highest level of the seven measured polyphenols was found in P. sapota, the second highest in P. viridis, and the lowest in P. campechiana. The levels of the seven polyphenols corresponded with the results of the 1,1-diphenyl-2-picrylhydrazyl assay, by which P. sapota had the highest antioxidant activity, P. viridis the second highest, and P. campechiana the lowest.
Ma
Jun
J
Department of Biological Sciences, Lehman College, and The Graduate Center, The City University of New York, 250 Bedford Park Boulevard West, Bronx, New York 10468, USA.
Yang
Hui
H
Basile
Margaret J
MJ
Kennelly
Edward J
EJ
D000975:Antioxidants / Q000032:analysis*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D032132:Pouteria / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols