title: [Hydrodynamic and biochemical adaptation to perfusion pressure profile in isolated hypothermic renal perfusion].
abstract: OBJECTIVE
  To evaluate hydrodynamic, biochemical and pathologic consequences of positive forced pressure on hypothermic isolated renal perfusions in comparison to softly progressive administration of perfusion solution.
  
  
  METHODS
  16 hypothermic experimental pig renal perfusion o 180 min completed under constant flow conditions. A vacuum computer-controlled pump was used. A comparison was performed of the results obtained by two groups of organs (forced perfusion pressure -I.B.- and soft perfusion pressure -I.S.).
  
  
  RESULTS
  Even though perfusion flor did not offer significant differences among both groups at the end of the procedure, I.B. group showed medium perfusion pressure and renal vascular resistance values significantly higher. Nitric oxide metabolites concentration was also greater in I.B. group. Finally, these kidneys showed more tubular cell aedema and glomerular shrinkage in comparison to I.S. group.
  
  
  CONCLUSIONS
  Sharp and forced hypothermic perfusion of the isolated kidney don't increase flow perfusion values. Inversely, hydrodynamic and pathologic results are worst in comparison to soft and progressive organ perfusion.
authors.affiliation: Servicio de Urología, Hospital General Universitario Gregorio Marañón, Madrid.
authors.forename: E
authors.initials: E
authors.lastname: Lledó García
authors.forename: C
authors.initials: C
authors.lastname: Hernández Fernández
authors.forename: J I
authors.initials: JI
authors.lastname: Martínez-Salamanca
authors.forename: M
authors.initials: M
authors.lastname: Moralejo Gárate
authors.forename: R
authors.initials: R
authors.lastname: Cabello Benavente
authors.forename: D
authors.initials: D
authors.lastname: Subirá Ríos
authors.forename: J F
authors.initials: JF
authors.lastname: del Cañizo López
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D006439:Hemodynamics / Q000502:physiology*; D007036:Hypothermia, Induced / Q000379:methods; D007668:Kidney / Q000098:blood supply* / Q000502:physiology*; D023421:Models, Animal; D012039:Regional Blood Flow / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature
vernacular_title: Perfusión hipotérmica del riñón aislado: respuesta hidrodinámica y bioquímica al perfil de aplicación de la presión.