Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues--a comparative immunohistochemical study.
The purpose of the present study was to compare the localization of prostaglandin E(2) receptor subtypes in normal human and mouse ocular tissues. Paraffin embedded sections of normal human and mouse (129 Sv/Ev) eyes were treated with EP(1), EP(2), EP(3) and EP(4) specific antibodies and subsequently incubated with Alexa Fluor secondary antibody (Ex/Em=555/571) to detect the presence of EP receptor proteins. Fluorescence of the localized antibodies was visualized in a Carl Zeiss Microscope (Axiovert 200) and photographed using Carl Zeiss Axiocam camera. In mice EP(1) and EP(3) receptor subtypes were only moderately expressed, EP(3) receptor expression being almost negligible. In human cornea and iris ciliary body, EP(1) and EP(3) receptors were prominently expressed. EP(4) receptor was expressed moderately in human and mouse ocular tissues. EP(2) receptor was the most prominently and abundantly expressed receptor in both human and mouse ocular tissues. It is concluded that the pattern of the distribution of EP receptor subtypes in the ocular tissues are similar in human and mouse. Thus, 129 Sv/Ev strains of mice would make an appropriate animal model for studying the ocular pathophysiological roles of prostaglandin receptor agonists.
Biswas
Sumana
S
Department of Ophthalmology and Visual Science, School of Medicine, University of Louisville, 301 E Muhammad Ali Blvd, Louisville, KY 40292, USA. s0bisw04@gwise.louisville.edu
Bhattacherjee
Parimal
P
Paterson
Christopher A
CA
D000818:Animals; D002829:Choroid / Q000737:chemistry; D002924:Ciliary Body / Q000737:chemistry; D003228:Conjunctiva / Q000737:chemistry; D003315:Cornea / Q000737:chemistry; D004727:Endothelium / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D005123:Eye / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007498:Iris / Q000737:chemistry; D051379:Mice; D010232:Paraffin; D018078:Receptors, Prostaglandin E / Q000032:analysis*; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D012160:Retina / Q000737:chemistry; D012590:Sclera / Q000737:chemistry; D016610:Tissue Embedding
C545635:PTGER1 protein, human; C545644:PTGER2 protein, human; C545647:PTGER3 protein, human; C545650:PTGER4 protein, human; C545636:Ptger1 protein, mouse; C545645:Ptger2 protein, mouse; C545648:Ptger3 protein, mouse; C545651:Ptger4 protein, mouse; D018078:Receptors, Prostaglandin E; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D010232:Paraffin