title: Degradation of aromatic hydrocarbons by Sphingomonas paucimobilis strain EPA505.
abstract: To determine the substrate range capability of Sphingomonas paucimobilis strain EPA505, a number of aromatic compounds were tested as potential growth substrates. Strain EPA505 grew on phenanthrene, naphthalene, fluoranthene, toluene, benzoic acid, 2,3- and 3,4-dihydroxybenzoic acids, 1-chloro-2,4-dinitrobenzene, anthracene, 2-hydroxy-3-naphthoic acid and 1-hydroxy- 2-naphthoic acid, salicylic acid, and catechol. Strain EPA505 was unable to grow on coumarine 3-carboxylic acid, naphthalene dicarboxylic acid, acenaphthene, chrysene, pyrene, benzo[b]fluoranthene, and fluorene. Catabolic products were not detected or identified when the bacterium was incubated with coumarine 3-carboxylic acid, naphthalene dicarboxylic acid, acenaphthene, chrysene, or benzo[b]fluoranthene. Dihydroxypyrene, the ortho ring fission product of pyrene, and 10-hydroxy-1- phenanthroic acid were detected when the bacterium was incubated with pyrene. The open rings of benzo[b]fluoranthene, hydroxyacephenanthroic acid, hydroxyacephenanthrene, and phenanthrene anhydride, catabolites of benzo[b]fluoranthene degradation, were detected with Tn5 mutants of EPA505. With strain EPA505, both 9-fluorenone and an open ring fission product accumulated during incubation with fluorene. Other catabolites beyond the open ring of fluorene were detected, specifically dihydroxyfluorene, hydroxy-9-fluorenone, dihydroxy-9-fluorenone, hydroxyindane, and a putative glutathione-conjugated benzylanhydride. Benzylanhydride appeared to be a final end product of fluorene degradation by strain EPA505.
authors.affiliation: Department of Biological Sciences/Microbiology, Clemson University, Clemson, South Carolina 29634-0314, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Story
authors.forename: E L
authors.initials: EL
authors.lastname: Kline
authors.forename: T A
authors.initials: TA
authors.lastname: Hughes
authors.forename: M B
authors.initials: MB
authors.lastname: Riley
authors.forename: S S
authors.initials: SS
authors.lastname: Hayasaka
mesh_terms: D008970:Molecular Weight; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism* / Q000633:toxicity; D020578:Sphingomonas / Q000254:growth & development / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical