title: Influence of PMN leukocyte-mediated pancreatic damage on the systemic immune response in severe acute pancreatitis in rats.
abstract: The outcome of severe acute pancreatitis is determined by the development of the systemic inflammatory response and subsequent multiorgan dysfunction. Using the taurocholate-induced model of acute pancreatitis in rats, we investigated the relationship between early polymorphonuclear (PMN)-mediated pancreatic tissue damage and the systemic inflammatory response. The respiratory burst of PMN leukocytes was increased in animals with acute pancreatitis and was reduced by anti-ICAM-1 antibody and oxygen radical scavenger treatment after 24 hr. In acute pancreatitis a reduced number of peripheral helper T cells was evident, most likely due to L-selectin-mediated increased lymphocyte homing. After 24 hr the CD45RC(high)/CD45RC(low) ratio of helper T cells, a critical factor in T cell-mediated disease was increased due to a reduction of regulatory CD45RC(low) cells. Only the treatment with anti-ICAM-1 mAb affected these changes, indicating that immunological changes in necrotizing pancreatitis are only in part affected by early PMN leukocyte-mediated pancreatic damage.
authors.affiliation: Department of General, Visceral, and Transplantation Surgery, University of Freiburg, 79106 Freiburg, Germany.
authors.forename: Uwe A
authors.initials: UA
authors.lastname: Wittel
authors.forename: Bettina
authors.initials: B
authors.lastname: Rau
authors.forename: Frank
authors.initials: F
authors.lastname: Gansauge
authors.forename: Susanne
authors.initials: S
authors.lastname: Gansauge
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Nussler
authors.forename: Hans G
authors.initials: HG
authors.lastname: Beger
authors.forename: Bertram
authors.initials: B
authors.lastname: Poch
mesh_terms: D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D004195:Disease Models, Animal; D007113:Immunity, Innate; D007249:Inflammation / Q000503:physiopathology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010195:Pancreatitis / Q000139:chemically induced / Q000276:immunology* / Q000503:physiopathology; D051381:Rats; D017382:Reactive Oxygen Species; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013656:Taurocholic Acid
chemical_list: D000911:Antibodies, Monoclonal; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1; D013656:Taurocholic Acid; D017493:Leukocyte Common Antigens