Rapid high-performance liquid chromatographic measurement of buspirone in human plasma after overdose.
For toxicological purposes, a rapid reversed-phase high-performance liquid chromatographic method was developed for the determination of the anxiolytic drug, buspirone, in human plasma. A liquid-liquid procedure was used to extract this compound from plasma in the presence of an internal standard, quinupramine. The analysis was performed on a Spherisorb S5 C8 analytical column with UV detection at 240 nm. No endogenous compounds were found to interfere. A linear response was observed over the concentration range 5-100 ng/mL. A good accuracy (bias < or =7.9%) was achieved for all quality controls, with intra-day and inter-day variation coefficients equal or less than 7.6%. The limit of quantification was 5 ng/mL. Stability of buspirone in plasma stored at different temperatures was checked. This rapid method (run time <12 min) was used to manage an acute poisoning involving buspirone.
Péhourcq
F
F
Laboratoire de Pharmacologie, Université Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France. fabienne.pehourcq@pharmaco.u-bordeaux2.fr
D002065:Buspirone / Q000097:blood* / Q000506:poisoning; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062787:Drug Overdose / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood* / Q000506:poisoning; D013056:Spectrophotometry, Ultraviolet
D017366:Serotonin Receptor Agonists; D002065:Buspirone