Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay.
To determine cypermethrin and permethrin in agricultural products, a competitive enzyme-linked immunosorbent assay (ELISA) method was employed. The matrix interferences were minimized by direct dilution of the extracts. No further cleanup was needed. A minimum matrix effect with a 1:10 dilution of white wine for cypermethrin and a 1:200 dilution of red and white wines, fruits, and vegetables for permethrin was found when phosphate-buffered saline containing 40% methanol was employed as the diluent. Good recoveries of spiked levels were observed. The mean percentage recoveries of cypermethrin spiked in white wine and permethrin spiked in red and white wines were 99.7, 74, and 78%, respectively. The mean percentage recoveries of permethrin spiked in apple, banana, cucumber, lettuce, onion, and peach were 99.2, 105, 70.2, 97.5, 94.4, and 89.4%, respectively. Validation of the ELISA method with permethrin-spiked lettuce and peach was carried out using gas chromatography with mass spectrometry, resulting in a good recovery and correlation.
Park
Eun-Kee
EK
Department of Entomology and UCD Cancer Research Center, University of California, Davis, California 95616, USA.
Kim
Jung-Hyun
JH
Gee
Shirley J
SJ
Watanabe
Takaho
T
Ahn
Ki Chang
KC
Hammock
Bruce D
BD
D000383:Agriculture; D004797:Enzyme-Linked Immunosorbent Assay; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D015203:Reproducibility of Results; D014675:Vegetables / Q000737:chemistry; D014920:Wine / Q000032:analysis
D010573:Pesticide Residues; D011722:Pyrethrins