title: [Preparation and purification of the antibody against ribonuclease inhibitor].
abstract: OBJECTIVE
  To prepare and purify the antibody against ribonuclease inhibitor(RI).
  
  
  METHODS
  RI was extracted from human placenta and purified by affinity chromatography. Rabbits anti-RI antibody was obtained after immunization and then purified through rProtein A Sepharose Fast Flow chromatography column. The characters of the antibody was identified by SDS-PAGE, ELISA and Western blot.
  
  
  RESULTS
  An anti-RI antibody was obtained and purified. SDS-PAGE analysis showed that the purified anti-RI antibody has high purity. The results of Western blot and ELISA indicated that anti-RI antibody had high specificity and good stability.
  
  
  CONCLUSIONS
  The anti-RI antibody with high titer, high specificity and good stability has been acquired, which lays the foundation for further research on RI.
authors.affiliation: Department of Pharmaceutical Chemistry, Dalian Medical University, Dalian 116023, China. schuxh@yahoo.com.cn
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Shu
authors.forename: Chuan-gang
authors.initials: CG
authors.lastname: Li
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Zhang
authors.forename: Jun-xia
authors.initials: JX
authors.lastname: Chen
authors.forename: Yu-xiang
authors.initials: YX
authors.lastname: Tian
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Yu
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: Cui
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D008297:Male; D010920:Placenta / Q000737:chemistry*; D010926:Placental Hormones / Q000276:immunology* / Q000302:isolation & purification; D011247:Pregnancy; D011817:Rabbits
chemical_list: D000906:Antibodies; D007106:Immune Sera; D010926:Placental Hormones; C022052:placental ribonuclease inhibitor