Multi-copy suppression of an Aspergillus nidulans mutant sensitive to camptothecin by a putative monocarboxylate transporter.
The Mono carboxylate Transporter (MCT) is a family of membrane proteins from the Major Facilitator Superfamily (MFS) of transporters. Here, we report the first identified Aspergillus nidulans MCT homologue, AmcA. The amcA gene was isolated by high copy number suppression of the hydroxyurea (HU) sensitivity of an A. nidulans camptothecin-sensitive mutant. Expression of amcA is increased when hyphae are grown in media containing acetate or pyruvate as single carbon source, and after exposure to several unrelated drugs. Our results suggest that AmcA could be involved not only in monocarboxylate transport, but also in drug secretion. To our knowledge, this is the first report suggesting a possible involvement of MCT transporters with drug resistance in eukaryotic cells.
Semighini
Camile P
CP
Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil.
Goldman
Maria Helena S
MH
Goldman
Gustavo Henrique
GH
D001233:Aspergillus nidulans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D001692:Biological Transport; D002166:Camptothecin / Q000494:pharmacology; D002264:Carboxylic Acids / Q000494:pharmacology; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D002264:Carboxylic Acids; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D027501:Monocarboxylic Acid Transporters; D002166:Camptothecin