Differential expression and function of IgA receptors (CD89 and CD71) during maturation of dendritic cells.
Dendritic cells (DC) are the most efficient antigen-presenting cells residing in mainly peripheral tissues. Antigen uptake by DC is particularly efficient, being mediated by various receptors such as lectin, scavenger receptors, and Fc receptors (FcRs). Immunoglobulin A (IgA) is part of the first-line immune barrier in mucosae, where DC are numerous. A member of the FcR family, FcalphaRI, is expressed on interstitial DC. We report here that monocyte-derived DC (Mo-DC) express another IgA receptor (IgA-R), the transferrin receptor (TfR), even in the absence of DC proliferation in vitro. Upon incubation with inflammatory cytokines such as tumor necrosis factor alpha and interleukin (IL)-1beta or maturating agents (lipopolysaccharide, CD40 ligand), FcalphaRI and TfR expression on Mo-DC was specifically up-regulated, whereas FcgammaRs and FcepsilonRI expression was down-regulated. Both IgA-Rs were functional, being able to mediate endocytosis by immature and activated Mo-DC. Although FcalphaRI internalized IgA complexes on both types of DC, TfR was only able to mediate IgA complex internalization by immature cells. Cross-linking of FcalphaRI but not of TfR resulted in up-regulation of major histocompatibility complex (MHC) class II/CD86 expression and secretion of IL-10 and IL-12 by immature Mo-DC. Moreover, in activated Mo-DC, cross-linking of FcalphaRI could up-regulated MHC class II/CD86 and triggered IL-10 secretion. Our findings led us to propose that FcalphaRI expressed by interstitial-type DC could play a critical role to sample IgA-recognized antigens and also during DC activation.
Pasquier
Benoit
B
INSERM E0225, Bichat Medical School, Paris, France.
Lepelletier
Yves
Y
Baude
Cédric
C
Hermine
Olivier
O
Monteiro
Renato C
RC
D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000187:drug effects / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000187:drug effects / Q000276:immunology*; D051940:B7-2 Antigen; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004705:Endocytosis / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D011961:Receptors, Fc / Q000187:drug effects / Q000276:immunology*; D017455:Receptors, IgE / Q000187:drug effects / Q000276:immunology; D017452:Receptors, IgG / Q000187:drug effects / Q000276:immunology; D011990:Receptors, Transferrin / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D051940:B7-2 Antigen; C068126:CD71 antigen; C497964:CD86 protein, human; D016207:Cytokines; C489017:FCGR1A protein, human; C036832:Fc(alpha) receptor; C045281:IgA receptor; D007070:Immunoglobulin A; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011961:Receptors, Fc; D017455:Receptors, IgE; D017452:Receptors, IgG; D011990:Receptors, Transferrin; D016753:Interleukin-10; D018664:Interleukin-12