Effect of sodium dodecyl sulfate micelles on peptide mass fingerprinting by matrix-assisted laser desorption/ionization mass spectrometry.
Here we have examined the effect of sodium dodecyl sulfate (SDS) at various concentrations on matrix-assisted laser desorption/ionization (MALDI) peptide mass fingerprinting experiments. Several model proteins were digested with trypsin and then various amounts of SDS were added prior to MALDI mass spectrometry. Evaluation of the data was made by calculating the amino acid sequence coverage within each analysis. It was found that addition of 0.1-0.3% w/v SDS prior to MALDI analysis results in an increase in the number of tryptic peptides detected thereby improving the total sequence coverage of the analysis. The use of SDS at concentrations near its critical micelle concentration can improve sequence coverage from MALDI peptide mass fingerprinting analyses allowing for increased confidence in protein identification or additional opportunities to identify putative regions of posttranslational modification.
Tummala
Rama
R
Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, PO Box 210172, University of Cincinnati, Cincinnati, OH 45221, USA.
Limbach
Patrick A
PA
D000595:Amino Acid Sequence; D000818:Animals; D003102:Colloids / Q000737:chemistry; D006736:Horses; D008823:Micelles; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009211:Myoglobin / Q000032:analysis / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012269:Ribosomal Proteins / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry*
D003102:Colloids; D008823:Micelles; D009211:Myoglobin; D011506:Proteins; D012269:Ribosomal Proteins; D013501:Surface-Active Agents; C029466:ribosomal protein L2; C015494:ribosomal protein S1; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin