title: Extensive antigenic changes in an atypical isolate of very virulent infectious bursal disease virus and experimental clinical control of this virus with an antigenically classical live vaccine.
abstract: The 99323 Egyptian isolate of infectious bursal disease (IBD) virus (IBDV) was identified during an international survey of acute IBD cases. Its unique antigenicity was characterized by a markedly reduced binding of neutralizing monoclonal antibodies 3, 4, 5, 6, 8 and 9 in an antigen-capture enzyme-linked immunosorbent assay. Nucleotide sequencing of the genome region encoding the VP2 major immunogenic domain in 99323 revealed amino acid changes occurring at positions critical for antigenicity, but phylogenetic analysis demonstrated that 99323 was related to typical, very virulent IBDV (e.g. isolate 89163). Protection experimentally afforded by an antigenically classical live IBD vaccine was investigated in specific pathogen free chickens challenged with 99323 or 89163. Both viruses were similarly controlled, as evaluated by clinical signs, growth retardation, bursa-to-body weight ratios and histological lesions of the bursa after challenge. These results document that an active antibody response to a classical live antigen may clinically control infection by an antigenically atypical very virulent IBDV.
authors.affiliation: Avian and Rabbit Virology, Immunology and Parasitology Unit (VIPAC), Ploufragan, France. n.eterradossi@ploufragan.afssa.fr
authors.forename: Nicolas
authors.initials: N
authors.lastname: Eterradossi
authors.forename: Céline
authors.initials: C
authors.lastname: Gauthier
authors.forename: Ismail
authors.initials: I
authors.lastname: Reda
authors.forename: Sylvain
authors.initials: S
authors.lastname: Comte
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Rivallan
authors.forename: Didier
authors.initials: D
authors.lastname: Toquin
authors.forename: Claire
authors.initials: C
authors.lastname: de Boisséson
authors.forename: Josiane
authors.initials: J
authors.lastname: Lamandé
authors.forename: Véronique
authors.initials: V
authors.lastname: Jestin
authors.forename: Yannick
authors.initials: Y
authors.lastname: Morin
authors.forename: Christophe
authors.initials: C
authors.lastname: Cazaban
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Borne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D011201:Poultry Diseases / Q000473:pathology / Q000517:prevention & control* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014611:Vaccination / Q000662:veterinary*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins