Enhanced oligonucleotide-directed gene targeting in mammalian cells following treatment with DNA damaging agents.
Targeted gene repair, a form of oligonucleotide-directed mutagenesis, employs end-modified single-stranded DNA oligonucleotides to mediate single-base changes in chromosomal DNA. In this work, we use a specific 72-mer to direct the repair of a mutated eGFP gene stably integrated in the genome of DLD-1 cells. Corrected cells express eGFP that can be identified and quantitated by FACS. The repair of this mutant gene is dependent on the presence of a specifically designed oligonucleotide and the frequency with which the mutation is reversed is affected by the induction of DNA damage. We used hydroxyurea, VP16 (etoposide), and thymidine to modulate the rate of DNA replication through the stalling of the replication forks or the introduction of lesions. Addition of hydroxyurea or VP16 before the electroporation of the oligonucleotide, results in an accumulation of double-strand breaks (DSB) whose repair is facilitated by either nonhomologous end joining (NHEJ) or homologous recombination (HR). The addition of thymidine results in DNA damage within replication forks, damage that is repaired through the process of homologous recombination. Our data suggest that gene repair activity is elevated when DNA damage induces or activates the homologous recombination pathway.
Ferrara
Luciana
L
Department of Biological Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.
Parekh-Olmedo
Hetal
H
Kmiec
Eric B
EB
D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects / Q000235:genetics; D018274:Electroporation; D005047:Etoposide / Q000494:pharmacology; D005434:Flow Cytometry; D018390:Gene Targeting; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed / Q000187:drug effects / Q000235:genetics; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics*; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics; D013936:Thymidine / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D009153:Mutagens; D009841:Oligonucleotides; D005047:Etoposide; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea