title: [The anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SMMC-7721 cells].
abstract: OBJECTIVE
  To explore the anti-cancer effects of siRNAs targeting hTERT in SMMC-7721 cells.
  
  
  METHODS
  Two siRNAs targeting hTERT mRNA were designed and synthesized by T7 transcription system in vitro. MMT, RT-PCR and Western blot were applied to evaluate effects on inhibiting cell growth, hTERT mRNA and protein expression in SMMC-7721 cells.
  
  
  RESULTS
  siRNAs decreased cell proliferation in a dose-dependent manner. At a concentration of 100 nmol/L, siRNAs exhibited obvious effects on inhibiting hTERT mRNA and protein expression in SMMC-7721 cells.
  
  
  CONCLUSIONS
  siRNAs targeting hTERT have significant inhibitory effects on hTERT gene expression in SMMC-7721 cells. siRNA has the possibility to become a new anti-cancer agent
authors.affiliation: Institute for Viral Hepatitis and the Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Su-Jun
authors.initials: SJ
authors.lastname: Zheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.forename: Sen
authors.initials: S
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Tao
authors.forename: Sheng-Qi
authors.initials: SQ
authors.lastname: Wang
mesh_terms: D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D018390:Gene Targeting; D015316:Genetic Therapy; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000235:genetics*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D019098:Telomerase