title: [Establishment and biological characteristics of orthotopic transplantation tumor model of hepatocellular carcinoma in mice].
abstract: OBJECTIVE
  To study the feasibility of the establishment of the orthotopic transplantation tumor model of hepatocellular carcinoma in mice and its tumor biological characteristics.
  
  
  METHODS
  H22 cells of hepatocellular carcinoma were inoculated to form ectopic transplanted model in mice by subcutaneous injection. Then the subcutaneous tumors were implanted into the liver of mice, and the orthotopic transplantation tumor model of hepatocellular carcinoma was established.
  
  
  RESULTS
  The successful rate of the orthotopic transplantation tumor model was 95.6% and the spontaneous metastatic rate was 81.8%, the rate of mass ascites was 40.9% and the natural extinctive rate was 0%. The natural survival time in the orthotopic transplantation tumor model was 28 days and the proliferation of tumor in transplanted model was accelerated after 2 weeks or so.
  
  
  CONCLUSIONS
  The orthotopic transplantation tumor model in mice is an ideal model for studying the metastatic mechanism and screening anti-tumor drugs for liver cancer, just because of its high successful rate and high spontaneous metastatic rate with no natural extinction.
authors.affiliation: Department of Tranditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. keqihan@yahoo.com
authors.forename: Ke-Qi
authors.initials: KQ
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Xia
authors.initials: X
authors.lastname: Hu
authors.forename: Ya-Ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Su
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Ling
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation / Q000379:methods