Production and quality of bovine oocytes and embryos.
Many factors influence the efficiency of the in vitro embryo production technology in cattle but the most important are the physiological conditions of the donor and the culture protocols for oocyte maturation and fertilization and for embryo culture from zygote to blastocyst. Therefore, general factors such as age, body conditions and herd management play a pivotal role together with more specific factors such as reproductive soundness and ovarian cyclicity. Given that good quality and competent oocytes are available a complex series of processes, including oocyte maturation, fertilization and culture of the derived zygotes, must be completed to generate viable embryos.
Galli
C
C
Laboratorio di Tecnologie della Riproduzione, CIZ srl, Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Porcellasco 7/F, 26100 Cremona, Italy.
Duchi
R
R
Crotti
G
G
Turini
P
P
Ponderato
N
N
Colleoni
S
S
Lagutina
I
I
Lazzari
G
G
D000818:Animals; D001757:Blastomeres / Q000166:cytology; D002417:Cattle; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D009865:Oocytes / Q000166:cytology*; D015053:Zygote / Q000166:cytology