title: Immunoprotection in goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions.
abstract: Haemonchus cysteine proteases, because of their apparent critical function in worm physiology, are considered important candidates in the immunological control of haemonchosis in sheep. Only limited information is, however, available on the immunoprotective properties of these molecules in goats. In the present study cysteine proteases of Haemonchus contortus adult worms isolated from a goat strain (Gran Canaria, Spain) were enriched by affinity chromatography and evaluated as immunoprotective antigens against caprine haemonchosis. The eggs per gram of faeces averaged over the whole experiment for unvaccinated goats (550 +/- 13.5) was significantly greater (P < 0.001) than that of vaccinated goats (61 +/- 2.9). Accordingly, the worm burden was significantly different between the groups (P < 0.05), with mean values of 247.5 +/- 43.8 and 762.5 +/- 78.3 worms per animal in the immunized and nonimmunized goats, respectively. The percentage of egg (89%) and worm (68%) reduction approached those attained with other immunogens used in sheep.
authors.affiliation: Department of Animal Pathology (Parasitology and Parasitic Diseases), Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35416 Arucas, Las Palmas, Spain. aruiz@dpat.ulpgc.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Ruiz
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Molina
authors.forename: Jorge Francisco
authors.initials: JF
authors.lastname: González
authors.forename: Magnolia María
authors.initials: MM
authors.lastname: Conde
authors.forename: Sergio
authors.initials: S
authors.lastname: Martín
authors.forename: Yeray Isidro
authors.initials: YI
authors.lastname: Hernández
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D006188:Haemonchiasis / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000201:enzymology / Q000276:immunology*; D008297:Male; D010270:Parasite Egg Count; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines
chemical_list: D000909:Antibodies, Helminth; D014612:Vaccines; D003546:Cysteine Endopeptidases