Expression and bioactivity analysis of recombinant beta-CPP dimer.
Beta-casein phosphopeptide (beta-CPP) is a bioactive peptide that carries different minerals, especially calcium. To investigate more effects of beta-CPP, eukaryotic expression vector of beta-CPP dimer was constructed and transfected into Chinese hamster ovary (CHO) cells. After selection, the cell lines stably expressing beta-CPP dimer were obtained, and the recombinant product was identified and purified. Activity assay of recombinant protein indicated that the recombinant beta-CPP dimer could improve Ca(2+) uptake of sperm, stimulate the proliferation of spleen cells, and induce apoptosis of some malignant tumor cells.
Jiang
Y J
YJ
Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin, Heilongjiang, 150030 China.
Li
Q Z
QZ
Yan
H B
HB
Geng
L J
LJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002364:Caseins / Q000235:genetics* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D019281:Dimerization; D015870:Gene Expression; D005822:Genetic Vectors; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000473:pathology; D016716:PC12 Cells; D010748:Phosphopeptides / Q000235:genetics* / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D014162:Transfection
D002364:Caseins; D010748:Phosphopeptides; D011994:Recombinant Proteins; D002118:Calcium