Interferon-gamma induces retinoic acid-inducible gene-I in endothelial cells.
Interferon-gamma (IFN-gamma) induces expression of multiple genes in endothelial cells. Retinoic acid-inducible gene-I (RIG-I) encodes a protein belonging to the DExH-box family, but details of its physiological function are not clear. RIG-I is induced in leukemia cells by retinoic acid and in endothelial cells by lipopolysaccharide. In the present study, the authors found that IFN-gamma also induces the expression of RIG-I in human umbilical vein endothelial cells. Induction of RIG-I mRNA by IFN-gamma was not altered by the treatment with cycloheximide or interleukin-4. Fluorescent immunostaining and Western blot analysis revealed cytoplasmic distribution of RIG-I. The in situ endothelium in a normal lung tissue was also found to express RIG-I protein. Although the physiological function of RIG-I is still unknown, induction of RIG-I by IFN-gamma may play an important role in inflammatory or immunological reactions in endothelial cells.
Imaizumi
T
T
Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan. timaizum@cc.hirosaki-u.ac.jp
Hatakeyama
M
M
Yamashita
K
K
Yoshida
H
H
Ishikawa
A
A
Taima
K
K
Satoh
K
K
Mori
F
F
Wakabayashi
K
K
D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism / Q000494:pharmacology*; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology; D008168:Lung / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020365:RNA Helicases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D011971:Receptors, Immunologic
D018836:Inflammation Mediators; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D015847:Interleukin-4; D007371:Interferon-gamma; D003513:Cycloheximide; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases