title: Stimulating the production of homoisoflavonoids in cell suspension cultures of Caesalpinia pulcherrima using cork tissue.
abstract: It has previously been demonstrated that cork tissue increases the efficiency of the production of lipophilic secondary metabolites in diverse plant cell suspension cultures. In the present study, three new homoisoflavonoids--named dihydrobonducellin, 2'-methoxydihydrobonducellin, and 2'-methoxybonducellin--and bonducellin and isobonducellin were isolated from Caesalpinia pulcherrima cultured cells coincubated with cork tissue. Cork tissue increased the production of 2'-methoxybonducellin by about 7-fold relative to control cells, and more than 80% of the product was recoverable from the cork tissue. When cork tissue and methyl jasmonate or yeast extract were added simultaneously to the medium, the amount of 2'-methoxybonducellin produced increased further. The production of the other four homoisoflavonoids was enhanced by variable amounts. Our results indicate that the addition of cork tissue would be an effective technique for investigating formation of secondary metabolites that usually accumulate only in trace amounts.
authors.affiliation: Course of Pharmaceutical Sciences, Medicinal Plant Garden, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521 Nagasaki, Japan.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Yuko
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Kouno
authors.forename: Yasukuni
authors.initials: Y
authors.lastname: Egami
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Yamamoto
mesh_terms: D000085:Acetates / Q000494:pharmacology; D029911:Caesalpinia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D007529:Isoflavones / Q000096:biosynthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D054883:Oxylipins; D035261:Plant Components, Aerial / Q000502:physiology*
chemical_list: C494250:2'-methoxybonducellin; C494248:2'-methoxydihydrobonducellin; D000085:Acetates; D003517:Cyclopentanes; D007529:Isoflavones; D054883:Oxylipins; C494249:dihydrobonducellin; C072239:methyl jasmonate