Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of ss-ketoesters.
New bacterial alcohol dehydrogenases with high and complementary enantioselectivity for the reduction of ethyl 3-keto-4,4,4-trifluorobutyrate 1 and methyl 3-keto-3-(3'-pyridyl)-propionate 3 have been rapidly identified by use of a new methodology consisting of preselection of microorganisms based on degradation ability and high-throughput screening with a miniaturized system coupled with fast analysis of enantioselectivities.
Zhang
Jie
J
Institute of Biotechnology, ETH-Zurich, Honggerberg, CH-8093 Zurich, Switzerland.
Duetz
Wouter A
WA
Witholt
Bernard
B
Li
Zhi
Z
D000150:Acinetobacter / Q000201:enzymology; D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D002384:Catalysis; D004952:Esters / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D007711:Klebsiella pneumoniae / Q000201:enzymology; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000201:enzymology; D013237:Stereoisomerism
D004952:Esters; D007659:Ketones; D000426:Alcohol Dehydrogenase