title: Easy method for determining the frequency of O(1) and O(2) alleles in Brazilian blood donors by PCR-RFLP analysis.
abstract: Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length polymorphisms (RFLPs) and DNA sequence studies offer the possibility of direct ABO genotyping. We used polymerase chain reaction-RFLP analysis to determine the frequency of O(1) and O(2) alleles in 82 unrelated blood donors in São Paulo, Brazil, known to be group O. Genomic DNA was extracted from blood leukocytes by a modified salting-out method. Different genotypes (O(1)O(1), O(1)O(2), O(2)O(2)) were identified after digestion with restriction enzymes KpnI, HpaII, and AluI, followed by agarose gel electrophoresis. Of 82 samples analyzed, 74 were O(1)O(1), 7 were O(1)O(2), and 1 was O(2)O(2). These results showed the frequency of O(1)O(1), O(1)O(2), and O(2)O(2) genotypes to be 90.24 percent, 8.53 percent, and 1.22 percent, respectively, in blood donors in São Paulo, Brazil.
authors.affiliation: Quality Control in Immunohematology Dept., Fundação Pró-Sangue/Hemocentro de São Paulo, Av. Dr. Eneas de Carvalho Aguiar 155, 1.andar, São Paulo, São Paulo, Brazil, CEP 05403-000.
authors.forename: A C
authors.initials: AC
authors.lastname: Batissoco
authors.forename: M C
authors.initials: MC
authors.lastname: Zago-Novaretti
authors.forename: V J
authors.initials: VJ
authors.lastname: Bueno
authors.forename: P E
authors.initials: PE
authors.lastname: Dorlhiac-Llacer
authors.forename: D A
authors.initials: DA
authors.lastname: Chamone