Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis.
We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
Lee
Soo-Ung
SU
Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Repubic of Korea.
Huh
Sun
S
D000818:Animals; D001483:Base Sequence; D002681:China; D003004:Clonorchis sinensis / Q000201:enzymology / Q000235:genetics*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D007723:Korea; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment; D013045:Species Specificity
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV