title: Dynamic evolution of the adenine nucleotide translocase interactome during chemotherapy-induced apoptosis.
abstract: The mitochondrial permeability transition pore complex (PTPC) is involved in the control of the mitochondrial membrane permeabilization during apoptosis, necrosis and autophagy. Indeed, the adenine nucleotide translocator (ANT) and the voltage-dependent anion channel (VDAC), two major components of PTPC, are the targets of a variety of proapoptotic inducers. Using co-immunoprecipitation and proteomic analysis, we identified some of the interacting partners of ANT in several normal tissues and human cancer cell lines. During chemotherapy-induced apoptosis, some of these interactions were constant (e.g. ANT-VDAC), whereas others changed strongly concomitantly with the dissipation of the mitochondrial transmembrane potential and until nuclear degradation occurred (e.g. Bax, Bcl-2, subunits of the respiratory chain, a subunit of the phosphatase PP2A, phospholipase PLC beta 4 and IP3 receptor). In addition, a glutathione-S-transferase (GST) interacts with ANT in normal tissue, in colon carcinoma cells and in vitro. This interaction is lost during apoptosis induction, suggesting that GST behaves as an endogenous repressor of PTPC and ANT pore opening. Thus, ANT is connected to mitochondrial proteins as well as to proteins from other organelles such as the endoplasmic reticulum forming a dynamic polyprotein complex. Changes within this ANT interactome coordinate the lethal response of cells to apoptosis induction.
authors.affiliation: CNRS FRE 2445, Université de Versailles/St Quentin, 45, avenue des Etats-Unis, Versailles 78035, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Verrier
authors.forename: Aurélien
authors.initials: A
authors.lastname: Deniaud
authors.forename: Morgane
authors.initials: M
authors.lastname: Lebras
authors.forename: Didier
authors.initials: D
authors.lastname: Métivier
authors.forename: Guido
authors.initials: G
authors.lastname: Kroemer
authors.forename: Bernard
authors.initials: B
authors.lastname: Mignotte
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Jan
authors.forename: Catherine
authors.initials: C
authors.lastname: Brenner
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005978:Glutathione / Q000502:physiology; D005982:Glutathione Transferase / Q000502:physiology; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D000226:Mitochondrial ADP, ATP Translocases / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D051381:Rats; D051028:bcl-2-Associated X Protein
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; C494828:Bax protein, rat; D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000226:Mitochondrial ADP, ATP Translocases; D005982:Glutathione Transferase; D005978:Glutathione