Migratory responses of murine hepatic myeloid, lymphoid-related, and plasmacytoid dendritic cells to CC chemokines.
Dendritic cell (DC) trafficking is regulated by chemokine receptor expression and responsiveness to chemokines. The authors compared CC chemokine receptor (CCR) expression by mouse liver myeloid, "lymphoid-related," and plasmacytoid DC subsets and their responsiveness to CC chemokines. CCR mRNA expression by liver DC subsets was evaluated by RNase protection assay. In vitro migration of the DC in response to recombinant murine CC chemokines was assayed using Transwell chambers. Immature liver DC did not respond to any CC chemokines tested, despite expression of mRNA encoding appropriate receptors for their ligands. CCR7 expression by each liver DC subset was strongly enhanced in response to maturation. The migratory capacity of liver plasmacytoid DC was similar to that of liver myeloid and lymphoid-related DC. These findings suggest that targeting of CCR7 and its ligands may be a potential approach for manipulation of liver DC trafficking to secondary lymphoid tissue after liver transplantation.
Abe
Masanore
M
Thomas E. Starzl Transplantation Institute, and Department of Surgery, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA.
Zahorchak
Alan F
AF
Colvin
Bridget L
BL
Thomson
Angus W
AW
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D019742:Chemokines, CC / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D008099:Liver / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010950:Plasma Cells / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D019707:Receptors, Chemokine / Q000235:genetics
D019742:Chemokines, CC; D012333:RNA, Messenger; D019707:Receptors, Chemokine