Metallo-beta-lactamase-producing Gram-negative bacilli in Korean Nationwide Surveillance of Antimicrobial Resistance group hospitals in 2003: continued prevalence of VIM-producing Pseudomonas spp. and increase of IMP-producing Acinetobacter spp.
Metallo-beta-lactamase (MBL)-producing Pseudomonas spp. and Acinetobacter spp. were prevalent in Korean hospitals. In this study, the prevalence and presence of MBL-producing isolates among imipenem-nonsusceptible and imipenem-susceptible isolates, respectively, were screened. The genetic and phenotypic characteristics of MBL-producing isolates were determined. Among imipenem-nonsusceptible isolates, 52 (11.1%) of 467 Pseudomonas spp. were blaVIM-like allele-positive, and 33 (15.1%) of 218 Acinetobacter spp. were either blaVIM- or blaIMP-like allele-positive. One blaVIM-like allele-positive isolate of Acinetobacter spp. was detected among 84 imipenem-susceptible Acinetobacter isolates. The minimum inhibitory concentration for 90% of isolates of imipenem was higher (>128 microg/mL) for Pseudomonas spp. than Acinetobacter spp. (16 microg/mL), although both had blaVIM-like allele. The source of MBL-producing isolates was mostly the sputum and urine of patients in the intensive care unit. Pulsed-field gel electrophoresis analysis suggested the intra- and inter-hospital spread of MBL-producing strains at some hospitals. In conclusion, blaVIM-like allele-positive P. aeruginosa remained highly prevalent, and the proportion of blaIMP-like allele-positive Acinetobacter spp. has increased significantly in most Korean hospitals.
Lee
Kyungwon
K
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.
Ha
Gyoung Yim
GY
Shin
Bo-Moon
BM
Kim
Jin Ju
JJ
Kang
Jung Oak
JO
Jang
Sook Jin
SJ
Yong
Dongeun
D
Chong
Yunsop
Y
D000150:Acinetobacter / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007723:Korea; D008297:Male; D008826:Microbial Sensitivity Tests; D015337:Multicenter Studies as Topic; D011159:Population Surveillance; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D015378:Imipenem; C417264:VIM-1 metallo-beta-lactamase; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases