title: [Production of shikonin by cell cultures of Lithospermum erythrorhizon].
abstract: OBJECTIVE
  To explore cultural conditions of shikonin production by cell cultures of Lithospermum erythrorhizon.
  
  
  METHODS
  Orthogonal design was applied in determination of shikonin within the medium. Flask test was applied in the study of shikonin production by the amount of ventilation.
  
  
  RESULTS
  The best medium consisted of 100 mg/L L-phenylalanine, 2 mg/L IAA and 800 mg/L Ca(NO3)2 4H2O. The best amount of ventilation was get by shaken at 150 r/min.
  
  
  CONCLUSIONS
  This test provided data for producing shikonin by cell cultures of Lithospermum erythrorhizon.
authors.affiliation: Guangdong Pharmaceutical College, Guangzhou 510220.
authors.forename: Liyong
authors.initials: L
authors.lastname: Hu
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured / Q000378:metabolism; D003470:Culture Media; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D029684:Lithospermum / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D009285:Naphthoquinones / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism / Q000494:pharmacology; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014691:Ventilation
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D009285:Naphthoquinones; C016101:shikonin; D010649:Phenylalanine; C030737:indoleacetic acid