Damaged DNA binding protein 1 in Drosophila defense reactions.
We have focused attention on functions of Drosophila damaged DNA binding protein 1 (D-DDB1) in Drosophila hematopoiesis and previously reported that its whole body dsRNA over-expression using a GAL4-UAS targeted expression system results in melanotic tumors and complete lethality. Since the lesions appear to arise as a normal and heritable response to abnormal development, forming groups of cells that are recognized by the immune system and encapsulated in melanized cuticle, D-DDB1 appears to be an essential development-associated factor in Drosophila. To probe the possibility that it contributes to hemocyte development, we used a collagen promoter-GAL4 strain to over-express dsRNA of D-DDB1 in Drosophila hemocytes. The D-DDB1 gene silencing caused melanotic tumors and mortality at the end of larval development. Similarly, it interfered with melanization and synthesis of antimicrobial peptides. Transgenic flies with D-DDB1 gene silencing were found to accumulate abnormal large blood cells, reminiscent of human leukemia, suggesting that D-DDB1 has functions in hemocyte development.
Takata
Kei-ichi
K
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan.
Shimanouchi
Kaori
K
Yamaguchi
Masamitsu
M
Murakami
Shizuka
S
Ishikawa
Gen
G
Takeuchi
Ryo
R
Kanai
Yoshihiro
Y
Ruike
Tatsushi
T
Nakamura
Ryou-ichi
R
Abe
Yoko
Y
Sakaguchi
Kengo
K
D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics / Q000276:immunology*; D004330:Drosophila / Q000166:cytology / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000172:deficiency* / Q000235:genetics / Q000276:immunology*; D004706:Endodeoxyribonucleases / Q000172:deficiency* / Q000235:genetics / Q000276:immunology*; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007113:Immunity, Innate / Q000276:immunology; D007938:Leukemia / Q000276:immunology* / Q000378:metabolism*; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D014018:Tissue Distribution
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D011994:Recombinant Proteins; D004706:Endodeoxyribonucleases; C112206:DdbP1 protein, Drosophila