The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway.
In the present study, we screened candidates for enhancing insulin action, using glucose uptake as an indicator, from Liriope platyphylla Wang et Tang (LPWT) extract, Liliaceae, in 3T3-L1 adipocytes. The mechanism of insulin sensitizing action in the fractions was also investigated. LPWT extract with 70% MeOH was sequentially separated with Diaion HP-20 and silica gel column chromatography. The 9:1 fraction from silica gel column chromatography increased glucose uptake with 1 ng/mL up to glucose uptake with 50 ng/mL insulin. The 9:1 fraction, determined as homoisoflavone-enriched fraction, worked as an insulin sensitizer. It increased insulin stimulated glucose uptake in 3T3-L1 adipocytes, insulin responsive cells, through increased glucose transporter 4 (GLUT4) contents in the plasma membrane. GLUT4 translocation was increased through insulin receptor substrate 1 (IRS1)-PI3 kinase-Akt signaling mechanism. Thus, homoisoflavone-enriched fraction in LPWT extract played an important role as an insulin sensitizer in adipocytes.
Choi
Soo Bong
SB
Department of Internal Medicine, Konkuk University, Kyohyun-Dong, Chungjoo-Si, Chungbuk-Do, South Korea.
Wha
Jun Dong
JD
Park
Sunmin
S
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D031424:Liriope Plant / Q000737:chemistry*; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D011493:Protein Kinase C / Q000502:physiology
D051275:Glucose Transporter Type 4; D007328:Insulin; D007529:Isoflavones; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D010936:Plant Extracts; C495693:Slc2a4 protein, mouse; D019869:Phosphatidylinositol 3-Kinases; C115015:PKC-3 protein; D011493:Protein Kinase C; D005947:Glucose