Characterization of an X-chromosome PCR-RFLP marker associated with fat deposition and growth in the pig.
The X-chromosome, highly conserved within mammals, has been shown to contain major quantitative trait loci (QTL) for growth and fat deposition in the pig. We have discovered a BamHI polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) marker that was assigned to the porcine X-chromosome by two-point and multi-point linkage analysis following genotyping of a three-generation Berkshire by Yorkshire reference family. The marker was positioned 9 cM telomeric to SW2126 and 15.6 cM centromeric to SW1943. Sequence flanking the marker was found to have high similarity to existing database porcine DNA repeat elements. Association analyses of the BamHI marker for growth and meat quality traits in the reference family revealed significant association with marbling (P < 0.03), 10th rib back fat (P < 0.09) and total lipid percentage (P < 0.05), as well as with loin eye area (P < 0.04), average glycolytic potential (P < 0.03) and average lactate content (P < 0.04). Further studies are required to determine the X-chromosome functional gene affecting fat deposition and growth in the pig.
Gaboreanu
A-M
AM
Department of Animal Science, Iowa State University, Ames 50011-3150, USA.
Grapes
L
L
Ramos
A M
AM
Kim
J-J
JJ
Rothschild
M F
MF
D000818:Animals; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics*; D017931:DNA Primers; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development; D020079:Terminal Repeat Sequences / Q000235:genetics; D014960:X Chromosome / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers