title: Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T cells.
abstract: Concomitant tumor immunity describes immune responses in a host with a progressive tumor that rejects the same tumor at a remote site. In this work, concomitant tumor immunity was investigated in mice bearing poorly immunogenic B16 melanoma. Progression of B16 tumors did not spontaneously elicit concomitant immunity. However, depletion of CD4(+) T cells in tumor-bearing mice resulted in CD8(+) T cell-mediated rejection of challenge tumors given on day 6. Concomitant immunity was also elicited by treatment with cyclophosphamide or DTA-1 monoclonal antibody against the glucocorticoid-induced tumor necrosis factor receptor. Immunity elicited by B16 melanoma cross-reacted with a distinct syngeneic melanoma, but not with nonmelanoma tumors. Furthermore, CD8(+) T cells from mice with concomitant immunity specifically responded to major histocompatibility complex class I-restricted epitopes of two melanocyte differentiation antigens. RAG1(-/-) mice adoptively transferred with CD8(+) and CD4(+) T cells lacking the CD4(+)CD25(+) compartment mounted robust concomitant immunity, which was suppressed by readdition of CD4(+)CD25(+) cells. Naturally occurring CD4(+)CD25(+) T cells efficiently suppressed concomitant immunity mediated by previously activated CD8(+) T cells, demonstrating that precursor regulatory T cells in naive hosts give rise to effective suppressors. These results show that regulatory T cells are the major regulators of concomitant tumor immunity against this weakly immunogenic tumor.
authors.affiliation: The Swim Across America Laboratory of Tumor Immunology, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, NY 10021, USA.
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Turk
authors.forename: José A
authors.initials: JA
authors.lastname: Guevara-Patiño
authors.forename: Gabrielle A
authors.initials: GA
authors.lastname: Rizzuto
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Engelhorn
authors.forename: Shimon
authors.initials: S
authors.lastname: Sakaguchi
authors.forename: Alan N
authors.initials: AN
authors.lastname: Houghton
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D003520:Cyclophosphamide / Q000494:pharmacology; D016654:Genes, RAG-1; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D016753:Interleukin-10 / Q000502:physiology; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D015375:Receptors, Interleukin-2; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003520:Cyclophosphamide