Phenotypic and genotypic characterization of Enterococcus spp. of different origins.
Sixty-four strains of Enterococcus spp. of different origin were identified using traditional phenotypic, biochemical and cultural tests, together with molecular tests. API 20 STREP tests identified the species at a preliminary level, only one strain remaining unidentified. Strains belonging to the genus Enterococcus were tested with genus-specific primers, while species-level identification was carried out with the 16S-23S rDNA intergenic region (ITS) and species-specific primers for E. faecium and E. faecalis. Those strains found to be negative with the species-specific primers were subjected to 16S rDNA partial sequencing.
Franzetti
Laura
L
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Agraria, Alimentare e Ecologica, Università di Milano, Via Celoria, 2, 20133 Milan, Italy. laura.franzetti@unimi.it
Pompei
Mariagrazia
M
Scarpellini
Mauro
M
Galli
Antonietta
A
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D002611:Cheese / Q000382:microbiology*; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S