title: Gene expression profile in liver of hB1F transgenic mice.
abstract: OBJECTIVE
  To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.
  
  
  METHODS
  Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR.
  
  
  RESULTS
  The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes.
  
  
  CONCLUSIONS
  The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.
authors.affiliation: Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
authors.forename: Shui-Liang
authors.initials: SL
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: You-Hua
authors.initials: YH
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Zhu-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Fu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D019076:Transgenes
chemical_list: D004268:DNA-Binding Proteins; C082631:NR5A2 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D015534:Trans-Activators; D014157:Transcription Factors