Microarray analysis in Alzheimer's disease and normal aging.
The purpose of this study was to investigate gene expression in Alzheimer's disease (AD), the most common form of senile dementia. We utilized the microarray technology to simultaneously compare the expression profile of 12,000 human genes in cerebral cortex of AD and normal aging. To identify gene expression related to neurodegeneration, beside the presence of amyloid deposition, we used control brains with abundant amyloid plaques, derived from cognitively normal elderly subjects. The microarray analysis indicated that 314 genes were differentially expressed in AD cerebral cortex, with differences greater than 5 folds in 25 genes. RT-PCR performed on a selected group of genes confirmed the increased expression of the interferon-induced protein 3 in AD brain. This protein, which is highly inducible by both type I and type II interferons, was not previously associated with the neurodegenerative disease.
Ricciarelli
Roberta
R
Department of Experimental Medicine, University of Genoa, via L.B. Alberti 2, 16132 Genoa, Italy. ricciarelli@medicina.unige.it
d'Abramo
Cristina
C
Massone
Sara
S
Marinari
Umberto
U
Pronzato
Maria
M
Tabaton
Massimo
M
D000368:Aged; D000375:Aging / Q000235:genetics*; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002540:Cerebral Cortex / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger