title: Simultaneous determination of thiamin and riboflavin in the sea urchin, Paracentrotus lividus, by high-performance liquid chromatography.
abstract: Thiamin and riboflavin were determined in the sea urchin, Paracentrotus lividus, by reversed-phase liquid chromatography with fluorescence detection and a mobile phase consisting of 72% 0.005 M NH(4)OAc and 28% MeOH at a flow rate of 1.450 ml/min. The total analysis time never exceeded 20 min. After acid hydrolysis and enzymatic digestion, thiamin was oxidized to thiochrome and the oxidized fraction was purified and concentrated by solid-phase extraction. Limits of detection were 9 pg/ml and 80 pg/ml for thiamin and riboflavin, respectively. Good results were obtained with respect to repeatability (RSD%<2.4) and recovery (>91.3%).
authors.affiliation: Nutrition and Bromatology Department, University of Santiago de Compostela, Santiago de Compostela, La Coruña, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Bernaldo De Quirós
authors.forename: J
authors.initials: J
authors.lastname: López-Hernández
authors.forename: J
authors.initials: J
authors.lastname: Simal-Lozano
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012256:Riboflavin / Q000032:analysis*; D012617:Sea Urchins / Q000737:chemistry*; D013831:Thiamine / Q000032:analysis*
chemical_list: D012256:Riboflavin; D013831:Thiamine