Identification of monovinyl tripropionic acid porphyrins and metabolites from faeces of patients with hereditary coproporphyria by high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
Harderoporphyrin (2-vinyl-4,6,7-tripropionic acid porphyrin) and its metabolites in faeces of patients with hereditary coproporphyria (HCP) have been separated and characterized by high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC/ESI-Q-TOFMS/MS). The metabolites identified were 2-ethyl-4,6,7-tripropionic acid porphyrin, 2-hydro-4,6,7-tripropionic acid porphyrin, 2-methoxyethyl-4,6,7-tripropionic acid porphyrin and 2-acetyl-4,6,7-tripropionic acid porphyrin. Isomers of harderoporphyrin derived from isomerization of harderoporphyrinogen were also detected.
Danton
Malcolm
M
MRC Bioanalytical Science Group, School of Biological & Chemical Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
Lim
Chang Kee
CK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D046349:Coproporphyria, Hereditary / Q000175:diagnosis* / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D011166:Porphyrins / Q000032:analysis* / Q000145:classification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D011166:Porphyrins; C040589:harderoporphyrin