Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines.
Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20 degrees C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.
Wang
P
P
Maastricht Proteomics Center, The Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands.
Mariman
E
E
Keijer
J
J
Bouwman
F
F
Noben
J-P
JP
Robben
J
J
Renes
J
J
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D020126:Brefeldin A / Q000494:pharmacology; D002454:Cell Differentiation; D015180:Electrophoresis, Gel, Two-Dimensional; D005109:Extracellular Matrix / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D015854:Up-Regulation
D015415:Biomarkers; D006133:Growth Substances; D011506:Proteins; D020126:Brefeldin A