A calcium-calmodulin antagonist blocks experimental Vibrio vulnificus cytolysin-induced lethality in an experimental mouse model.
We demonstrated that trifluoperazine, a calcium-calmodulin antagonist, blocked the hyperpermeability induced by Vibrio vulnificus cytolysin in in vitro-modeled endothelium and prevented the deaths of mice. Furthermore, compared to tetracycline alone, tetracycline combined with trifluoperazine enhanced the survival rate of V. vulnificus-infected mice, indicating the role of the cytolysin as an important factor in pathogenesis.
Lee
Young-Rae
YR
Department of Biochemistry, Chonbuk National University Medical School, Chonju, Republic of Korea.
Park
Kwang-Hyun
KH
Lin
Zhao-Zhen
ZZ
Kho
Young-Jong
YJ
Park
Jin-Woo
JW
Rho
Hye-Won
HW
Koo
Bon-Sun
BS
Kim
Hyung-Rho
HR
Song
Eun-Kyung
EK
Yu
Hong-Nu
HN
Han
Myung-Kwan
MK
Lee
Seung-Ok
SO
Jhee
Eun-Chung
EC
Kim
Jong-Suk
JS
D000418:Albumins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects*; D002460:Cell Line; D003603:Cytotoxins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D021381:Protein Transport / Q000187:drug effects; D014268:Trifluoperazine / Q000494:pharmacology*; D041261:Vibrio vulnificus / Q000737:chemistry / Q000472:pathogenicity* / Q000502:physiology
D000418:Albumins; D002147:Calmodulin; D003603:Cytotoxins; D014268:Trifluoperazine; D002118:Calcium