Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA.
RNA digestion by RNase H, which is responsible for the antisense effect, was efficiently photoregulated by use of the duplex of azobenzene-tethered sense DNA and native antisense DNA. In the dark, RNA digestion was suppressed because antisense DNA was strongly hybridized with azobenzene-tethered sense DNA, and accordingly RNA was isolated. On UV irradiation, antisense DNA was released from the azobenzene-tethered DNA due to the trans-to-cis isomerization and hybridized with RNA, which was digested by RNase H.
Matsunaga
Daijiro
D
Research Center for Advance Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Asanuma
Hiroyuki
H
Komiyama
Makoto
M
D001391:Azo Compounds / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D010777:Photochemistry; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016914:Ribonuclease H / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D001391:Azo Compounds; D012313:RNA; D004247:DNA; D016914:Ribonuclease H; C009850:azobenzene