[Effect of anti-human VEGF monoclonal antibody on VEGF level in serum of nude mice transplanted buccal carcinoma: a quantitative study].
OBJECTIVE
Quantitative study of the effect of anti-human VEGF mAb E11 to VEGF level in serum of nude mice transplanted buccal carcinoma.


METHODS
E11 was administered into BALB/c nu/nu mice which were transplanted human buccal carcinoma. The saline was administrated as negative control. Mice were killed at 18 days. The VEGF level in serum of mice was determined by improved indirect ELISA.


RESULTS
Compared with the VEGF level in serum of mice in saline group, it was dramatically decreased in E11 group. The VEGF level in serum of mice treated E11 by subcutaneous was lowest and only reached (1.17 +/- 0.13) microg/L.


CONCLUSIONS
It demonstrated that the anti-human VEGF mAb could reduce the VEGF level in serum by binding VEGF, and block its biological activity. It indicates that VEGF in serum of malignant tumor patient is a new tumor marker.
Fang
Si-lian
SL
Dept. of Oral and Maxillofacial Surgery, the Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai 519000, China.
Wang
Da-zhang
DZ
Zhang
Jing-yi
JY
Zheng
Guang-yong
GY
Yang
Xi-chuan
XC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000097:blood; D002277:Carcinoma / Q000097:blood*; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000473:pathology*; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000097:blood*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse