title: An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion.
abstract: Phospholipase Cgamma1 (PLCgamma1) has been reported to be expressed predominantly in T cells and to play an important role in T-cell receptor signaling. Here we show that PLCgamma1 is expressed throughout B-cell development, with high expression in B-cell progenitors, and is involved in pre-B-cell receptor (pre-BCR) signaling. Reduced expression of PLCgamma1, in the absence of PLCgamma2 (PLCgamma1+/-PLCgamma2-/-), impedes early B-cell development at the pro-B- to pre-B-cell transition and impairs immunoglobulin heavy chain allelic exclusion, hallmarks of defective pre-BCR signaling. In contrast, early B-cell development is largely normal, whereas late B-cell maturation is impaired in the absence of PLCgamma2 alone (PLCgamma2-/-) and overexpression of PLCgamma1 in PLCgamma2-/- mice fails to restore BCR-mediated B-cell proliferation and maturation. These studies reveal an essential role of PLCgamma1, distinct from that of PLCgamma2, in B-cell development.
authors.affiliation: The Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Renren
authors.initials: R
authors.lastname: Wen
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Chen
authors.forename: James
authors.initials: J
authors.lastname: Schuman
authors.forename: Guoping
authors.initials: G
authors.lastname: Fu
authors.forename: Shoua
authors.initials: S
authors.lastname: Yang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.forename: Debra K
authors.initials: DK
authors.lastname: Newman
authors.forename: Demin
authors.initials: D
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000502:physiology*; D003433:Crosses, Genetic; D006412:Hematopoietic Stem Cells / Q000201:enzymology* / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D051966:Phospholipase C gamma; D010738:Type C Phospholipases / Q000172:deficiency* / Q000378:metabolism*
chemical_list: D010738:Type C Phospholipases; D051966:Phospholipase C gamma