title: Cell fusion activities of Hantaan virus envelope glycoproteins.
abstract: Hantaan virus (HTNV)-infected Vero E6 cells undergo cell fusion with both infected and uninfected cells under low-pH conditions. Flow cytometry and fluorescence microscopy of HTNV-infected Vero E6 cells showed that envelope glycoproteins (GPs) were located both on the cell surface and in the cytoplasm. Neutralizing monoclonal antibodies (MAbs) against the G1 and G2 envelope GPs inhibited cell fusion, whereas nonneutralizing MAbs against G1 or G2 and MAbs against the nucleocapsid protein (NP) did not. Transfected Vero E6 cells that expressed GPs but not those that expressed NP fused and formed syncytia. These results indicate that HTNV GPs act as fusogens at the cell surface. No fusion activity was observed either in infected Vero cells that were passaged more than 150 times or in BHK-21 cells, although GPs appeared to localize to the cell surface. This variability in fusion induction suggests the involvement of host cell factors in the process of cell membrane fusion.
authors.affiliation: Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Ogino
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Hideki
authors.initials: H
authors.lastname: Ebihara
authors.forename: Koichi
authors.initials: K
authors.lastname: Araki
authors.forename: Byoung-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Megumi
authors.initials: M
authors.lastname: Okumura
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000914:Antibodies, Viral / Q000378:metabolism; D002459:Cell Fusion; D002462:Cell Membrane / Q000737:chemistry; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D015726:Giant Cells / Q000166:cytology / Q000821:virology; D006023:Glycoproteins / Q000502:physiology*; D018063:Hantaan virus / Q000472:pathogenicity / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D014760:Viral Fusion Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C090197:glycoprotein G2, Hantavirus