title: Chimeric LNA/DNA probes as a detection system for real-time PCR.
abstract: OBJECTIVE
  Evaluation of the use of short probes as a detection system in real-time PCR.
  
  
  METHODS
  Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum.
  
  
  RESULTS
  The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal.
  
  
  CONCLUSIONS
  Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
authors.affiliation: M. Dassault Molecular Biology Laboratory, Centre de Diagnostic Prénatal, American Hospital of Paris, Neuilly, 92200 Neuilly-sur-Seine, France. jean-marc.costa@ahparis.org
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Costa
authors.forename: Pauline
authors.initials: P
authors.lastname: Ernault
authors.forename: Martine
authors.initials: M
authors.lastname: Olivi
authors.forename: Thierry
authors.initials: T
authors.lastname: Gaillon
authors.forename: Khalil
authors.initials: K
authors.lastname: Arar
mesh_terms: D041322:Chromosomes, Human, Y; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics; D010949:Plasma / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; C477371:locked nucleic acid