title: Biodegradable poly(ethylene glycol) hydrogels crosslinked with genipin for tissue engineering applications.
abstract: In this study amino-terminated poly(ethylene glycol) (PEG-diamine) hydrogels were crosslinked with genipin, a chemical naturally derived from the gardenia fruit. Dissolution, swelling, and PEG-genipin release properties were determined. The dissolution studies indicated that the hydrogels are water soluble, and that the dissolution rate was concentration, mass, and temperature dependent. The dissolution rates are easily tailored from 3 min to >100 days. The PEG-genipin release study indicated that the greatest release occurs within the first 24 h of immersion in water, and that incubation at 37 degrees C elicits a greater initial release than samples incubated at room temperature for all genipin concentrations. Through scanning electron microscopy it was observed that the hydrogels are porous, and surface morphology changes before and after swelling. Furthermore, smooth muscle cell (SMC) adhesion studies indicated that the PEG-genipin hydrogel is a suitable substrate for SMC seeding. Overall, the results of these studies indicate that PEG-genipin hydrogels may provide potential scaffolding for a variety of tissue engineering applications.
authors.affiliation: Department of Materials Science and Engineering, Carnegie Mellon University, 5000 Forbes Ave., Pittsburgh, Pennsylvania 15213, USA.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Moffat
authors.forename: Kacey G
authors.initials: KG
authors.lastname: Marra
mesh_terms: D000269:Adhesives; D000818:Animals; D001011:Aorta; D001672:Biocompatible Materials; D002448:Cell Adhesion; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D057889:Iridoid Glycosides; D039823:Iridoids; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology; D011092:Polyethylene Glycols / Q000737:chemistry*; D011714:Pyrans; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D057889:Iridoid Glycosides; D039823:Iridoids; D011714:Pyrans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C007834:genipin