title: Quantification of water diffusion and relaxation times of human U87 tumors in a mouse model.
abstract: Assessing the potential of anti-cancer agents can be greatly facilitated by applying MRI methods to investigations with animal models. Quantitative diffusion imaging, T1, and T2 measurements may offer valuable information for understanding properties of the tumor and for evaluating new therapeutic approaches. The human U87 high-grade glial tumor is widely used for cancer investigations in orthotopic murine models. The physiological features of this model at the cellular and sub-cellular level have not, however, been well characterized by MRI. In this study, we measured the diffusion, T1 and T2 characteristics of water in the human U87 tumor at 8.5 T in an orthotopic murine model in vivo and analyzed their detailed changes in the transition from the tumor core through the tumor periphery, and out to surrounding tissue using custom developed radial profile analysis software. For the tumor bearing mice (n = 10), the mean average apparent diffusion coefficient (ADC) of the tumor core was 1.03 +/- 0.02 ( x 10(-3) mm2/s), while in the contralateral normal brain it was 0.73 +/- 0.03 ( x 10(-3) mm2/s). The mean T1 in tumor was 2.03 +/- 0.08 s and in normal brain tissue was 1.64 +/- 0.06 s. The mean T(2) in tumor was 0.062 +/- 0.002 s and in normal brain tissue was 0.048 +/- 0.001 s. The mean ADC, T1 and T2 of the tumor compared to normal tissue were significantly different (p < 0.005).
authors.affiliation: Department of Radiology, Brigham and Women's Hospital, Boston, MA 02115, USA. ysun@bwh.harvard.edu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Sun
authors.forename: Robert V
authors.initials: RV
authors.lastname: Mulkern
authors.forename: Karl
authors.initials: K
authors.lastname: Schmidt
authors.forename: Sameer
authors.initials: S
authors.lastname: Doshi
authors.forename: Mitchell S
authors.initials: MS
authors.lastname: Albert
authors.forename: Nils O
authors.initials: NO
authors.lastname: Schmidt
authors.forename: Mateo
authors.initials: M
authors.lastname: Ziu
authors.forename: Peter
authors.initials: P
authors.lastname: Black
authors.forename: Rona
authors.initials: R
authors.lastname: Carrol
authors.forename: Mark W
authors.initials: MW
authors.lastname: Kieran
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001932:Brain Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000378:metabolism*; D045744:Cell Line, Tumor; D004058:Diffusion; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005910:Glioma / Q000737:chemistry / Q000175:diagnosis* / Q000378:metabolism*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008657:Metabolic Clearance Rate; D051379:Mice; D008819:Mice, Nude; D014867:Water / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D014867:Water