title: The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens.
abstract: The rumen represents the first section of a ruminant animal's stomach, where feed is collected and mixed with microorganisms for initial digestion. The major gas produced in the rumen is CO(2) (65.5 mol%), yet the metabolic characteristics of capnophilic (CO(2)-loving) microorganisms are not well understood. Here we report the 2,314,078 base pair genome sequence of Mannheimia succiniciproducens MBEL55E, a recently isolated capnophilic Gram-negative bacterium from bovine rumen, and analyze its genome contents and metabolic characteristics. The metabolism of M. succiniciproducens was found to be well adapted to the oxygen-free rumen by using fumarate as a major electron acceptor. Genome-scale metabolic flux analysis indicated that CO(2) is important for the carboxylation of phosphoenolpyruvate to oxaloacetate, which is converted to succinic acid by the reductive tricarboxylic acid cycle and menaquinone systems. This characteristic metabolism allows highly efficient production of succinic acid, an important four-carbon industrial chemical.
authors.affiliation: Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Rese Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Jin Sik
authors.initials: JS
authors.lastname: Kim
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: In
authors.forename: Sun Shim
authors.initials: SS
authors.lastname: Choi
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Rih
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Kim
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Jeong
authors.forename: Cheol Goo
authors.initials: CG
authors.lastname: Hur
authors.forename: Jae Jong
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D016680:Genome, Bacterial; D031121:Mannheimia / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D002245:Carbon Dioxide; D019802:Succinic Acid