Detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay.
Arakawa
Hidetoshi
H
School of Pharmaceutical Sciences, Department of Analytical Chemistry, Showa University, Tokyo 142-8555, Japan. arakawa@pharm.showa-u.ac.jp
Karasawa
Koji
K
Igarashi
Takeshi
T
Suzuki
Shigeya
S
Goto
Nobuichi
N
Maeda
Masako
M
D000483:Alleles; D002138:Calibration; D003773:Dental Plaque / Q000382:microbiology*; D011756:Diphosphates / Q000032:analysis / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D007202:Indicators and Reagents / Q000032:analysis; D007700:Kinetics; D008163:Luminescent Measurements; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013295:Streptococcus mutans / Q000235:genetics*; D016986:Streptococcus sobrinus / Q000235:genetics*; D013379:Substrate Specificity
D011756:Diphosphates; D007202:Indicators and Reagents