A subnanogram assay for phospholipase activity based on a long-chain radioiodinatable phosphatidylcholine.
Caramelo
Julio J
JJ
Institute of Biophysics and Biochemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, RA-1113 Buenos Aires, Argentina. jcaramelo@leloir.org.ar
Delfino
José M
JM
D000327:Adsorption; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D017815:Elapidae; D006868:Hydrolysis; D007457:Iodine Radioisotopes; D007700:Kinetics; D008832:Microchemistry; D015394:Molecular Structure; D036103:Nanotechnology; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D012822:Silicon Dioxide; D013379:Substrate Specificity
D007457:Iodine Radioisotopes; D010713:Phosphatidylcholines; D012822:Silicon Dioxide; D010740:Phospholipases