Nucleofection of muscle-derived stem cells and myoblasts with phiC31 integrase: stable expression of a full-length-dystrophin fusion gene by human myoblasts.
Quenneville
Simon P
SP
Unité de Recherche en Génétique Humaine, Centre de Recherche du CHUL, CHUQ, Faculté de Médecine, Université Laval, Sainte-Foy, Québec, Canada, G1V 4G2.
Chapdelaine
Pierre
P
Rousseau
Joël
J
Beaulieu
Jean
J
Caron
Nicolas J
NJ
Skuk
Daniel
D
Mills
Philippe
P
Olivares
Eric C
EC
Calos
Michele P
MP
Tremblay
Jacques P
JP
D000818:Animals; D019677:Artificial Gene Fusion; D001287:Attachment Sites, Microbiological / Q000235:genetics; D001435:Bacteriophages / Q000201:enzymology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016189:Dystrophin / Q000032:analysis / Q000235:genetics*; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D032446:Myoblasts / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
D016189:Dystrophin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019426:Integrases