Substrates and inhibitors of efflux proteins interfere with the MTT assay in cells and may lead to underestimation of drug toxicity.
Vellonen
Kati-Sisko
KS
Department of Pharmaceutics, University of Kuopio, Kuopio, PO Box 1627, 70211 Kuopio, Finland.
Honkakoski
Paavo
P
Urtti
Arto
A
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000166:Acridines / Q000494:pharmacology; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010084:Oxidation-Reduction; D011422:Propionates / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D044005:Tetrahydroisoquinolines / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014700:Verapamil / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000166:Acridines; D002121:Calcium Channel Blockers; D007770:L-Lactate Dehydrogenase; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011422:Propionates; D011804:Quinolines; D044005:Tetrahydroisoquinolines; D013778:Tetrazolium Salts; D013844:Thiazoles; D014700:Verapamil; C059141:verlukast; C022616:thiazolyl blue; C083501:Elacridar; C421109:multidrug resistance-associated protein 1