title: Recombinant expression, purification and cross-reactivity of chenopod profilin: rChe a 2 as a good marker for profilin sensitization.
authors.affiliation: Depto. Bioquímica y Biología Molecular, Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.forename: Mayte
authors.initials: M
authors.lastname: Villalba
authors.forename: Rosalía
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D000485:Allergens / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000918:Antibody Specificity; D015415:Biomarkers; D027462:Chenopodium / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003285:Contractile Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D003429:Cross Reactions; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics*; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D013058:Mass Spectrometry; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010936:Plant Extracts; D010944:Plants / Q000737:chemistry; D011058:Pollen / Q000737:chemistry; D051304:Profilins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000485:Allergens; D015415:Biomarkers; D003285:Contractile Proteins; D018076:DNA, Complementary; D007074:Immunoglobulin G; D008840:Microfilament Proteins; D010936:Plant Extracts; D051304:Profilins; D011994:Recombinant Proteins; D007073:Immunoglobulin E