Effects of a novel disulfide bond and engineered electrostatic interactions on the thermostability of azurin.
Tigerström
Anna
A
Department of Chemistry, Lundberg Institute, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden.
Schwarz
Frederick
F
Karlsson
Göran
G
Okvist
Mats
M
Alvarez-Rúa
Carmen
C
Maeder
Dennis
D
Robb
Frank T
FT
Sjölin
Lennart
L
D001120:Arginine / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics; D001400:Azurin / Q000737:chemistry* / Q000235:genetics*; D002152:Calorimetry, Differential Scanning; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000235:genetics; D004220:Disulfides / Q000138:chemical synthesis*; D008239:Lysine / Q000235:genetics; D013058:Mass Spectrometry; D016297:Mutagenesis, Site-Directed; D011489:Protein Denaturation / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization; D013053:Spectrophotometry; D055672:Static Electricity; D013816:Thermodynamics; D015032:Zinc / Q000737:chemistry
D004220:Disulfides; D001400:Azurin; D001224:Aspartic Acid; D003300:Copper; D001120:Arginine; D015032:Zinc; D008239:Lysine; D003545:Cysteine