Development and automation of a 384-well cell fusion assay to identify inhibitors of CCR5/CD4-mediated HIV virus entry.
Bradley
Joe
J
Laboratory Automation Group, Pfizer Global Research and Development, Kent, UK.
Gill
Jasween
J
Bertelli
Francois
F
Letafat
Sara
S
Corbau
Romu
R
Hayter
Paul
P
Harrison
Paula
P
Tee
Andy
A
Keighley
Wilma
W
Perros
Manos
M
Ciaramella
Giuseppe
G
Sewing
Andreas
A
Williams
Christine
C
D000818:Animals; D001665:Binding Sites; D001681:Biological Assay / Q000379:methods*; D065100:CCR5 Receptor Antagonists; D015704:CD4 Antigens / Q000378:metabolism*; D016466:CHO Cells; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D015696:Gene Products, tat / Q000378:metabolism; D019302:HIV Envelope Protein gp160 / Q000378:metabolism; D023581:HIV Fusion Inhibitors / Q000032:analysis* / Q000494:pharmacology; D015497:HIV-1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D019713:Receptors, CCR5 / Q000378:metabolism*; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014162:Transfection; D001616:beta-Galactosidase / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus
D065100:CCR5 Receptor Antagonists; D015704:CD4 Antigens; D015696:Gene Products, tat; D019302:HIV Envelope Protein gp160; D023581:HIV Fusion Inhibitors; D010446:Peptide Fragments; D019713:Receptors, CCR5; D054322:tat Gene Products, Human Immunodeficiency Virus; C415751:tat peptide (1-9), Human immunodeficiency virus 1; D001616:beta-Galactosidase