Mutation of amino acids in the alpha 1,3-fucosyltransferase motif affects enzyme activity and Km for donor and acceptor substrates.
Jost
Franziska
F
Department of Chemistry and Biochemistry, 1600 Holloway Ave., San Francisco State University, San Francisco, CA 94132, USA.
de Vries
Theodora
T
Knegtel
Ronald M A
RM
Macher
Bruce A
BA
D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D001665:Binding Sites / Q000235:genetics; D017124:Conserved Sequence; D005647:Fucosyltransferases / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007700:Kinetics; D017354:Point Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics; D013379:Substrate Specificity
D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase