High-throughput methods for measuring heparanase activity and screening potential antimetastatic and anti-inflammatory agents.
Huang
Kuo-Sen
KS
Department of Discovery Technologies, Hoffmann-La Roche Inc., 340 Kingsland Street, Nutley, NJ 07110, USA. kuo-sen.huang@roche.com
Holmgren
Janna
J
Reik
Linda
L
Lucas-McGady
Debra
D
Roberts
John
J
Liu
Chao-Min
CM
Levin
Wayne
W
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001710:Biotin / Q000378:metabolism; D016466:CHO Cells; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D015870:Gene Expression; D005966:Glucuronidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006497:Heparan Sulfate / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000201:enzymology; D013194:Staining and Labeling; D014316:Tritium; D000099175:Heparanase
D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D001710:Biotin; D005346:Fibroblast Growth Factors; D016650:Fluorescein-5-isothiocyanate; D005966:Glucuronidase; D006497:Heparan Sulfate; D014316:Tritium; D000099175:Heparanase