[Inter-laboratory evaluation studies for development of notified ELISA methods for allergic substances (milk)].
Extracts of sausage, sauce, cookie, cereal and pasta sauce spiked with milk standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of the three ELISA methods using a Milk Protein Casein ELISA Kit (Casein kit), a Milk Protein Beta-Lactoglobulin ELISA Kit (Beta-Lactoglobulin kit) and a FASTKIT Milk ELISA Kit (Milk ELISA kit) were mostly below 10%. Mean recoveries of the milk standard protein from the food extracts were over 40% in the three ELISA methods with a few excertions. The recoveries of milk standard protein from the sauce extract in Casein kit were improved by adjusting the extract to neutrality before the Casein kit assay. The recoveries of milk standard protein from cookie, cereal and pasta sauce were improved by the increasing the amount of antibody coated in the Milk ELISA kit. The detection limits of all the ELISA methods were 1 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of milk protein levels in extracts of sausage, sauce, cookie, cereal and pasta sauce.
Akiyama
Hiroshi
H
National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Isuzugawa
Kazuto
K
Harikai
Naoki
N
Watanabe
Hiroko
H
Iijima
Ken
K
Yamakawa
Hirohito
H
Mizuguchi
Yamato
Y
Yoshikawa
Reiji
R
Yamamoto
Miho
M
Sato
Hidetaka
H
Watai
Masatoshi
M
Arakawa
Fumihiro
F
Ogasawara
Takeshi
T
Nishihara
Rikuka
R
Kato
Hisashi
H
Yamauchi
Atsushi
A
Takahata
Yoshihisa
Y
Morimatsu
Fumiki
F
Mamegoshi
Shinichi
S
Muraoka
Shiroo
S
Honjoh
Tsutomu
T
Watanabe
Takahiro
T
Sakata
Kozue
K
Imamura
Tomoaki
T
Toyoda
Masatake
M
Matsuda
Rieko
R
Maitani
Tamio
T
D000485:Allergens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008894:Milk Proteins / Q000032:analysis*; D015337:Multicenter Studies as Topic; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
D000485:Allergens; D008894:Milk Proteins; D011933:Reagent Kits, Diagnostic