Development of a nonmechanical enucleation method using x-ray irradiation in somatic cell nuclear transfer.
Irradiation of in-vitro-matured bovine oocytes with x-rays of different durations was performed to develop an alternative to conventional mechanical enucleation methods in somatic cell nuclear transfer. No significant difference in embryo development to the blastocyst stage was detected between nonmechanical and mechanical methods, and cytologic analyses of karyotype and microtubule formation showed the potential availability of x-ray irradiation.
School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Tae M
TM
Kim
Woo S
WS
Hwang
Ji H
JH
Shin
Hyun J
HJ
Park
Jae Y
JY
Han
Jeong M
JM
Lim
D000818:Animals; D002417:Cattle / Q000196:embryology*; D002459:Cell Fusion / Q000662:veterinary*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D014965:X-Rays