Apolipoprotein E polymorphisms in Japanese patients with polypoidal choroidal vasculopathy and exudative age-related macular degeneration.
OBJECTIVE
To study the genotypes, allelic frequencies, and polymorphisms of apolipoprotein E (Apo E) in unrelated Japanese patients with polypoidal choroidal vasculopathy (PCV) or exudative age-related macular degeneration (AMD) and control subjects without macular degeneration.


METHODS
Cross-sectional study.


METHODS
Blood samples from 225 subjects older than 50 years were used. The 225 subjects included 58 patients with PCV, 85 with AMD, and 82 without macular degeneration. Coding exons of the Apo E gene were amplified by polymerase chain reaction, and the DNA sequences were determined by direct sequencing with an automated sequencer.


RESULTS
Apo E epsilon3/epsilon3 was the most frequent genotype with a prevalence of 79.3% in PCV patients, 76.5% in AMD patients, and 67.1% in the control subjects. However, the differences in the percentages were not statistically significant among the three groups. The most frequently found allele in the three groups was epsilon3. Patients with PCV and AMD were less likely to have epsilon2 and epsilon4 than the control subjects, but the differences were not statistically significant. Five minor Apo E single nucleotide polymorphisms, including epsilon5 and epsilon7, were found.


CONCLUSIONS
Japanese patients with PCV and AMD were less likely to have epsilon2 and epsilon4 polymorphisms, but the differences from the normals were not statistically significant for the Apo E genotypes and allelic frequencies.
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
Norimoto
N
Gotoh
Sachiko
S
Kuroiwa
Takanobu
T
Kikuchi
Jun
J
Arai
Satoko
S
Arai
Noriko
N
Yoshida
Nagahisa
N
Yoshimura
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics*; D002829:Choroid / Q000098:blood supply*; D015862:Choroid Diseases / Q000208:ethnology / Q000235:genetics*; D003430:Cross-Sectional Studies; D004247:DNA / Q000032:analysis; D005260:Female; D005451:Fluorescein Angiography; D005787:Gene Frequency; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008268:Macular Degeneration / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D001057:Apolipoproteins E; D004247:DNA