Rapid and economical method for biochemical screening of stool isolates for Salmonella and Shigella species.
The LOUIS test is a screening protocol for Salmonella and Shigella that uses rapid enzyme tests. It had a sensitivity of 100% and a specificity of 94% and achieved presumptive reporting of Salmonella 3 h after colony isolation with savings of time and money compared to commercial identification systems.
Department of Medical Microbiology, Stirling Royal Infirmary, Stirling, Scotland, United Kingdom. gwilson44@btinternet.com
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D000465:Algorithms; D015373:Bacterial Typing Techniques / Q000191:economics*; D002262:Carboxy-Lyases / Q000378:metabolism; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007211:Indoles / Q000378:metabolism; D009598:Nitrophenylgalactosides / Q000378:metabolism; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012760:Shigella / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors; D014508:Urea / Q000378:metabolism
D003470:Culture Media; D007211:Indoles; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; C030374:indole; D014508:Urea; D002262:Carboxy-Lyases; C021520:lysine decarboxylase