[Construction of the subtracted cDNA library in hypothalamus of the seasickness adaptive rats].
OBJECTIVE
To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.


METHODS
Suppression subtract hybridization technique was used, and forward and reverse hybridization was performed on the hypothalamus of seasickness adaptive rats and that of normal rats so that to construct subtracted cDNA library. Dot blot was used for differential screening the subtracted library.


RESULTS
23 fragments of differentially expressed genes was obtained including 10 up-regulating and 13 down-regulating fragments.


CONCLUSIONS
Many played role in adaptability formation to seasickness such as SAM, vasopressin, and heme oxygenase.
Department of Military Hygiene, the Second Military Medical University, Shanghai 200433, China.
Yanhong
Y
Ji
Junsheng
J
Guo
Min
M
Li
Faji
F
Zhao
D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D005786:Gene Expression Regulation; D015723:Gene Library; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009041:Motion Sickness / Q000235:genetics / Q000503:physiopathology*; D009693:Nucleic Acid Hybridization; D051381:Rats; D017207:Rats, Sprague-Dawley
D018076:DNA, Complementary