Endocytic host cell machinery plays a dominant role in intracellular trafficking of incoming human immunodeficiency virus type 1 in human placental trophoblasts.
Vertical transmission of human immunodeficiency virus type 1 (HIV-1) is the primary cause of infection by this retrovirus in infants. In this study, we report for the first time that there is a correlation between endocytic uptake of HIV-1 and virus gene expression in polarized trophoblasts. To shed light on the relationship between endocytosis and the fate of HIV-1 in polarized trophoblasts, the step-by-step movements of HIV-1 within the endocytic compartments were tracked by confocal imaging. Incoming virions were initially located in early endosomes. As time progressed, virions accumulated in late endosomes. HIV-1 was also found in apical recycling endosomes and at the basolateral pole. Experiments performed with indicator cells revealed that HIV-1 is recycled and transcytosed. These data indicate that the intracellular trafficking of HIV-1 upon entry into polarized human trophoblasts is a complex process which requires the active participation of the endocytic host cell machinery.
Vidricaire
Gaël
G
Research Center in Infectious Diseases, RC709, CHUL Research Center, Faculty of Medicine, Laval University, Quebec, Canada.
Imbeault
Michael
M
Tremblay
Michel J
MJ
D015703:Antigens, CD / Q000032:analysis; D002460:Cell Line; D016764:Cell Polarity; D004705:Endocytosis; D011992:Endosomes / Q000821:virology; D005260:Female; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D009068:Movement; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D060149:Tetraspanin 30; D014327:Trophoblasts / Q000821:virology*; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000032:analysis; D000099193:rab11 GTP-Binding Proteins
D015703:Antigens, CD; D008565:Membrane Proteins; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C554586:CD63 protein, human; C093846:early endosome antigen 1