T cell proliferation by direct cross-talk between OX40 ligand on human mast cells and OX40 on human T cells: comparison of gene expression profiles between human tonsillar and lung-cultured mast cells.
Mast cells (MCs) are the primary effector cells in allergic reactions and have also been found to activate T cells and to reside in close physical proximity to T cells. However, the molecular mechanisms involved in the MC-T cell interaction remain unclear. We hypothesized that human tonsillar MCs, which locate in the interfollicular areas, might interact with T cells. Thus, we first established a culture system of human tonsillar MCs and then compared gene expression profiles of tonsillar MCs with that of lung MCs before and after aggregation of FcepsilonRI by using high-density oligonucleotide probe arrays. Here we show that resting tonsillar MCs, when compared with lung MCs, revealed significantly higher expression levels for CC chemokines (CCL3 and 4), which recruit T cells, and for TNFR superfamilies (OX40 ligand and 4-1BB ligand), which induce proliferation of T cells. After aggregation of FcepsilonRI, not only tonsillar MCs but also lung MCs up-regulated the expression of these molecules. We confirmed that T cell proliferation is induced in direct cross-talk by the MC surface molecule OX40 ligand. These results suggest that human MCs may play important roles in adaptive immunity through the T cell responses.
Kashiwakura
Jun-ichi
J
Research Unit for Allergy Transcriptome, Research Center for Allergy and Immunology, The Institute of Physical and Chemical Research, (RIKEN) Yokohama Institute, Suehiro-cho, Tsurumi-ku, Japan.
Yokoi
Hidenori
H
Saito
Hirohisa
H
Okayama
Yoshimichi
Y
D053330:4-1BB Ligand; D015703:Antigens, CD; D002478:Cells, Cultured; D018920:Coculture Techniques; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000166:cytology*; D008213:Lymphocyte Activation; D008407:Mast Cells / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000502:physiology*; D053278:OX40 Ligand; D014066:Palatine Tonsil / Q000166:cytology*; D017455:Receptors, IgE / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000502:physiology; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D053330:4-1BB Ligand; D015703:Antigens, CD; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; D017455:Receptors, IgE; D017475:Receptors, Nerve Growth Factor; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor; C505716:TNFRSF4 protein, human; C505714:TNFRSF9 protein, human; C089251:TNFSF4 protein, human; C505974:TNFSF9 protein, human; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D014409:Tumor Necrosis Factor-alpha