Chronic implantation of transit-time flow probes on the ascending aorta of rodents.
This study describes the implantation of transit-time flow probes on the ascending aorta of rats while minimizing the risk of postoperative complications. Special emphasis is placed on our new method of rat intubation as well as the production of materials necessary for the implantation procedure such as endotracheal tubes and heparin bonded vessel catheters. The effects of these devices on the response to acute hypoxia were studied in rats following a 5-7 day recovery from the implantation procedure. Systemic and microvascular measurements were made on instrumented rats (n = 5) and non-instrumented controls (n = 3) that were ventilated with 21%, 15%, 10%, 8% and 5% oxygen. Arterial pressure, PO(2), lactate, and base deficit were not different between the implanted and control animals at any inspired oxygen concentration. Microvascular flow in the primary arterioles of the spinotrapezius muscle was also similar between the two groups at all inspired oxygen concentrations. We conclude that this novel methodology facilitates the measurement of whole body oxygen delivery in resting and haemodynamically-stressed rats.
Department of Physiology, Virginia Commonwealth University Reanimation Engineering and Shock Center, Virginia Commonwealth University Health System, Richmond, Virginia 23298, USA.
Lane
L
Smith
M Hakam
MH
Tiba
Mary Ellen
ME
Goldberg
R Wayne
RW
Barbee
D000818:Animals; D001011:Aorta / Q000502:physiology / Q000601:surgery*; D001783:Blood Flow Velocity / Q000502:physiology / Q000662:veterinary; D001784:Blood Gas Analysis / Q000662:veterinary; D001794:Blood Pressure / Q000502:physiology; D007440:Intubation / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D008866:Microsurgery / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods / Q000662:veterinary; D011182:Postoperative Care; D051381:Rats; D011921:Rats, Inbred WKY / Q000601:surgery*; D012039:Regional Blood Flow / Q000502:physiology
D019344:Lactic Acid