Regulation of inflammatory responses.
Leukocytes play an important role in host defense and inflammatory disease. The effects of leukocytes are mediated by bacterial peptide, n-formyl-Met-Leu-Phe (fMLP), which interacts with a G protein-related kinase receptor to produce acute (degranulation) and chronic (chemokine production) inflammatory mediators. The purpose of this study was to determine the role of fMLP receptor phosphorylation by G protein-related kinase, GRK2, on degranulation and chemokine production. Here we show over-expression of GRK2 decreases degranulation and increases chemokine production. These studies suggest that receptor phosphorylation plays a unique role in "turning off" acute inflammation and simultaneously "turning on" a chronic inflammatory response. This study has relevance to many inflammatory diseases, including many in the oral cavity.
Krug
Ari
A
Ahamed
Jasimuddin
J
Ali
Hydar
H
D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation / Q000276:immunology; D018932:Chemokine CCL2 / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000276:immunology; D006801:Humans; D007249:Inflammation; D018836:Inflammation Mediators / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D010766:Phosphorylation; D044042:Receptors, Formyl Peptide / Q000276:immunology; D051552:beta-Adrenergic Receptor Kinases
D018932:Chemokine CCL2; D018836:Inflammation Mediators; D044042:Receptors, Formyl Peptide; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases; D002118:Calcium