Induction of RNA interference in dendritic cells.
Dendritic cells (DC) reside at the center of the immunological universe, possessing the ability both to stimulate and inhibit various types of responses. Tolerogenic/regulatory DC with therapeutic properties can be generated through various means of manipulations in vitro and in vivo. Here we describe several attractive strategies for manipulation of DC using the novel technique of RNA interference (RNAi). Additionally, we overview some of our data regarding yet undescribed characteristics of RNAi in DC such as specific transfection strategies, persistence of gene silencing, and multi-gene silencing. The advantages of using RNAi for DC genetic manipulation gives rise to the promise of generating tailor-made DC that can be used effectively to treat a variety of immunologically mediated diseases.
Li
Mu
M
Department of Surgery, Microbiology and Immunology, The University of Western Ontario, Canada.
Qian
Hua
H
Ichim
Thomas E
TE
Ge
Wei-Wen
WW
Popov
Igor A
IA
Rycerz
Katarzyna
K
Neu
John
J
White
David
D
Zhong
Robert
R
Min
Wei-Ping
WP
D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics / Q000276:immunology; D016219:Immunotherapy, Adoptive; D034622:RNA Interference / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology*; D014162:Transfection
D034741:RNA, Small Interfering