Developmental expression of SNAP-25 protein in the rat striatum and cerebral cortex.
The developmental changes of 25-kDa synaptosomal-associated protein (SNAP-25) expression in the rat striatum and cerebral cortex were examined using Western- blotting and densitometric scanning of immunoblots. Analysis of the striatum extracts from postnatal day 0 (P0) to postnatal day 120 (P120) demonstrated that SNAP-25 is poorly expressed until P14. From this point the expression level gradually increases to reach a maximum on P60 and then decreases. The pattern of SNAP-25 expression in the rat cerebral cortex is different. Two peaks are observed, the first on P10 and the second on P60, after which the expression level decreases. These results appear to confirm the role of SNAP-25 protein in axon outgrowth and synaptogenesis in the nervous system.
Department of Anatomy and Neurobiology, Medical University, Gdańsk, Poland. jusid@biology.pl
Justyna
J
Sidor-Kaczmarek
Cezary
C
Labuda
Barbara
B
Litwinowicz
Jan Henryk
JH
Spodnik
Przemysław
P
Kowiański
Jerzy
J
Dziewiatkowski
Janusz
J
Moryś
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D003342:Corpus Striatum / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D050825:Synaptosomal-Associated Protein 25
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25