Influence of DnaK and DnaJ chaperones on Escherichia coli membrane lipid composition.
The content of fatty acids extracted from the membranes of E. coli MC 1061 harboring the wild-type dnaKdnaJ alleles and its delta dnaJ and delta dnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42 degrees C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and octadecanol, the latter being correlated with the presence of octadecanoic acid. The amount of LPS extracted from delta dnaKdanJ mutant was significantly lower when compared with wild-type strain and delta dnaJ mutant.
Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Justyna
J
Sieńczyk
Aleksandra
A
Skłodowska
Anna
A
Grudniak
Krystyna I
KI
Wolska
D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D015964:Gene Expression Regulation, Bacterial; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry*; D018832:Molecular Chaperones / Q000378:metabolism; D009154:Mutation
C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008563:Membrane Lipids; D018832:Molecular Chaperones; C063842:dnaK protein, E coli