Large-scale carbohydrate analysis by capillary array electrophoresis: part 1. Separation and scale-up.
A 96-capillary array electrophoresis (CAE) instrument has been adapted for large-scale mono- and oligosaccharide analysis and characterization. Operational protocols and data processing tools have been developed to optimize the CAE system for this application. Effects of different additives to the running buffer on efficiency and capillary-to-capillary performance reproducibility have been studied.
Khandurina
Julia
J
Diversa Corporation, San Diego, CA 92121, USA. jkhandurina@diversa.com
Olson
Nels A
NA
Anderson
Abraham A
AA
Gray
Kevin A
KA
Guttman
Andras
A
D002021:Buffers; D002241:Carbohydrates / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results
D002021:Buffers; D002241:Carbohydrates