[Methods of hygromycin B phosphotransferase activity assay in transgenic plant].
Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the development of various transgenic plants, silence of inserted gene, marker-free system development and safety assessment of transgenic food. In this paper, several methods for detecting the activity of this enzyme were reviewed.
Zhuo
Qin
Q
Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Yang
Xiaoguang
X
D020868:Gene Silencing; D005819:Genetic Markers; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics
D005819:Genetic Markers; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase