[Evaluation of bacterial flora in contaminated soil as a countermeasure against H2S gas production].
Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, spore-forming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium.
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Hatsumi
H
Taniguchi
Kazumasa
K
Fukuda
Wan
W
Yan
Mitsuo
M
Hinoue
Kazuki
K
Yamauchi
Tsuyoshi
T
Ichihara
Kouhei
K
Mizuno
Sumiyo
S
Ishimatsu
Nobuyuki
N
Sera
Mitsuhiro
M
Hamasaki
Kouji
K
Takahashi
Kazumi
K
Horikawa
D000397:Air Pollution / Q000517:prevention & control*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D003199:Computer Systems; D005740:Gases; D006862:Hydrogen Sulfide / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D014866:Waste Products
D005740:Gases; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014866:Waste Products; D006862:Hydrogen Sulfide