Isolation and characterization of a cellulolytic Geobacillus thermoleovorans T4 strain from sugar refinery wastewater.
A novel, cellulolytic, bacterial thermophilic strain, T4, was isolated from sugar refinery wastewater in southern Taiwan. This isolate, a Gram-negative, motile, aerobically growing sporulating rod, can secrete thermostable endocellulase (endo-1,4-beta-D-glucanase, EC 3.2.1.4) and hydrolyze carboxymethylcellulose (CMC), phosphoric acid-swollen cellulose, Avicel, filter paper, and salicin. When strain T4 was grown in CMC medium, the cellulolytic enzyme activity in culture supernatants was stable up to 70 degrees C. More than 10% of the original activity was still detectable after heating to 100 degrees C with a pH 7.0 for 1 h. Based on 16S rDNA sequence analysis, DNA base composition, phenotypic and physiological characteristics, as well as DNA-DNA hybridization, strain T4 was classified as Geobacillus thermoleovorans T4 (DSM 14791 = CCRC 17200). We also demonstrated that the type species G. stearothermophilus (DSM 22 = ATCC 12980) could hydrolyze amorphous and crystalline (filter paper) celluloses at a rate of 13 and 14%, respectively, in comparison with strain T4.
Tai
Shang-Kai
SK
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan, 804, ROC.
Lin
Hsiu-Ping Pearl
HP
Kuo
Jimmy
J
Liu
Jong-Kang
JK
D001406:Bacillaceae / Q000145:classification / Q000502:physiology*; D001592:Benzyl Alcohols / Q000737:chemistry; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005960:Glucosides; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors
D001592:Benzyl Alcohols; D004275:DNA, Ribosomal; D005960:Glucosides; C005696:salicin; D002482:Cellulose; D004247:DNA; D002266:Carboxymethylcellulose Sodium