Reverse effects of tetraarsenic oxide on the angiogenesis induced by nerve growth factor in the rat cornea.
To compare the antiangiogenic effects of tetraarsenic oxide (As4O6) with those of diarsenic oxide (As2O3) in the rat cornea, rat cornea micropocket assay was conducted to induce angiogenesis by implantation of the pellet contained 1.0 ng of nerve growth factor (NGF). Ten of thirty eyes of Sprague-Dawley rats were randomly assigned to one of three groups, namely, control group (no medication), As2O3 group (50 mg/kg As2O3, PO, s.i.d.), and As4O6 group (50 mg/kg As4O6, PO, s.i.d.). After implantation, the number of new vessels, vessel length and clock hour of neovascularization were examined under the microscope from day 3 to day 7. The area of neovascularization was calculated using a mathematical formula. Although new vessels in control and As2O3 groups were first noticed at day 3, whereas those of As4O6 group were first observed on day 5. The number, length, clock hour of neovascularization and areas of the vessels in As4O6 group showed more significant inhibition than those of control and As2O3 groups from day 5 (P<0.05). However, there were no differences in all parameters between control group and As2O3 group during the entire study period. These results showed that As4O6 had antiangiogenic effects on the new vessels induced by NGF in the rat cornea.
Department of Veterinary Medicine, Kangwon National University, Chuncheon, Seoul, Republic of Korea.
Myung-Hyun
MH
Yoo
Jong-Taek
JT
Kim
Chang-Hun
CH
Rhee
Myung-Jin
MJ
Park
Ill-Ju
IJ
Bae
Na-Young
NY
Yi
Man-Bok
MB
Jeong
Seong-Mok
SM
Jeong
Tchi-Chou
TC
Nam
Kang-Moon
KM
Seo
D000704:Analysis of Variance; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000737:chemistry / Q000627:therapeutic use*; D016510:Corneal Neovascularization / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D008297:Male; D008853:Microscopy; D020932:Nerve Growth Factor / Q000633:toxicity*; D010087:Oxides / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D020533:Angiogenesis Inhibitors; D001152:Arsenicals; D010087:Oxides; D020932:Nerve Growth Factor; D000077237:Arsenic Trioxide