Comparison of rDNA sequences from colchicine treated and untreated sporocysts of Phyllodistomum folium and Bucephalus polymorphus (Digenea).
The most frequently used antimitotic agent in cytogenetic studies is colchicine. We investigated whether the initial treatment of trematodes for karyological analysis with colchicine would have mutagenic or degradational effect on rDNA sequences. Dreissena polymorpha is the intermediate host of Phyllodistomum folium and Bucephalus polymorphus, and the sporocyst stage of these trematode species develop, respectively, in the gills and gonads of this mussel. Sporocysts of P. folium and B. polymorphus were obtained from D. polymorpha collected from waterbodies in Belarus and in Lithuania. 5.8S and 28S rDNA genes, ITS1 and ITS2 of P folium and B. polymorphus were sequenced and compared, and no nucleotide sequence differences between colchicine treated and untreated trematodes were found. Based on these results, we conclude that colchicine treatment for 3-5 h has no mutagenic or degradational effect on rDNA sequences. During the course of this investigation, two genetically different P. folium samples were noted in Belarus.
Institute of Ecology, Vilnius University, Akademijos 2, Vilnius LT 2600, Lithuania. stunzen@ekoi.lt
Virmantas
V
Stunzenas
Jason R
JR
Cryan
Daniel P
DP
Molloy
D000818:Animals; D049872:Bivalvia / Q000469:parasitology; D003078:Colchicine / Q000494:pharmacology*; D018509:DNA, Helminth / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D008969:Molecular Sequence Data; D033661:Oocysts / Q000187:drug effects* / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; D003078:Colchicine