[Identification of a protein interacting with Shigella flexneri IpaC invasin by yeast two-hybrid system].
Two-hybrid system was applied to screen proteins interacting with IpaC in the host cell. By using two-hybrid system, the bait plasmid containing ipaC gene was constructed and designated pGBKT-IpaC. A human HeLa cDNA library was screened to isolate protein factors that might interact with IpaC. Among the 2 x 10(6) clones screened, 22 positive clones were picked out. Sequence analysis revealed that two of them contained cDNA fragments from collagenase. Subsequently the domain of IpaC interacting with collagenase fragment was identified. These results suggest that IpaC might play a role in some biological processes where collagenase is involved.
Beijing Institute of Biotechnology, Beijing 100071, China. xyyan@vip.sina.com
Xiao-Yu
XY
Yan
Xiao
X
Yao
Heng-Liang
HL
Wang
Er-Ling
EL
Feng
Zhao-Xing
ZX
Shi
Guo-Fu
GF
Su
Song
S
You
Liu-Yu
LY
Huang
D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D012763:Shigella flexneri / Q000235:genetics*; D020798:Two-Hybrid System Techniques
D000942:Antigens, Bacterial; C092342:IpaC protein, Shigella; D017364:Collagenases