Axenic culture of Leishmania amastigotes.
One of the future goals in Leishmania research will be to reproduce the entire life cycle oxenically, in vitro. In this article, Paul Bates reviews recent progress in the axenic culture of amastigotes and addresses some of the remaining problems associated with culture methods for both amostigote and promostigote forms.
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Laboratory for Biochemical Parasitology, Department of Zoology, University of Glasgow, Glasgow, UK G12 8QQ.