On-line single droplet deposition for MALDI mass spectrometry.
A single droplet generator was coupled with a rotating ball inlet matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometer. Single droplets with 100 picoliter volume were ejected by a piezoelectric-actuated droplet generator and deposited onto a matrix-coated rotating stainless steel ball at atmospheric pressure. The single droplet deposit was transported to the vacuum side of the instrument where ionization was accomplished using a UV pulsed laser. Using this on-line interface, it was possible to obtain protonated molecule signal from as little as 10 fmol analyte.
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA.
Xia
X
Zhang
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA.
Damien A
DA
Narcisse
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA. kkmurray@lsu.edu.
Kermit K
KK
Murray
D000803:Angiotensin I / Q000737:chemistry; D000818:Animals; D001331:Automation; D001920:Bradykinin / Q000737:chemistry; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D010316:Particle Size; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D007328:Insulin; D045304:Cytochromes c; D000803:Angiotensin I; D001920:Bradykinin