Overexpression of a cell adhesion molecule, TSLC1, as a possible molecular marker for acute-type adult T-cell leukemia.
Adult T-cell leukemia (ATL) caused by human T-cell leukemia virus type 1 (HTLV-1) infection, occurs in 2% to 4% of the HTLV-1 carriers with a long latent period, suggesting that additional alterations participate in the development of ATL. To characterize and identify novel markers of ATL, we examined the expression profiles of more than 12 000 genes in 8 cases of acute-type ATL using microarray. One hundred ninety-two genes containing interleukin 2 (IL-2) receptor alpha were up-regulated more than 2-fold compared with CD4(+) and CD4(+)CD45RO(+) T cells, and tumor suppressor in lung cancer 1 (TSLC1), caveolin 1, and prostaglandin D2 synthase showed increased expression of more than 30-fold. TSLC1 is a cell adhesion molecule originally identified as a tumor suppressor in the lung but lacks its expression in normal or activated T cells. We confirmed ectopic expression of the TSLC1 in all acute-type ATL cells and in 7 of 10 ATL- or HTLV-1-infected T-cell lines. Introduction of TSLC1 into a human ATL cell line ED enhanced both self-aggregation and adhesion ability to vascular endothelial cells. These results suggested that the ectopic expression of TSLC1 could provide a novel marker for acute-type ATL and may participate in tissue invasion, a characteristic feature of the malignant ATL cells.
Department of Biochemistry, Miyazaki Medical College, University of Miyazaki, Kiyotake, Miyazaki, Japan.
Hidenori
H
Sasaki
Ichiro
I
Nishikata
Toshiyuki
T
Shiraga
Ena
E
Akamatsu
Takeshi
T
Fukami
Tomonori
T
Hidaka
Yoko
Y
Kubuki
Akihiko
A
Okayama
Kenji
K
Hamada
Hisafumi
H
Okabe
Yoshinori
Y
Murakami
Hirohito
H
Tsubouchi
Kazuhiro
K
Morishita
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D002460:Cell Line; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D025521:Tumor Suppressor Proteins
D014408:Biomarkers, Tumor; C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; D025521:Tumor Suppressor Proteins