Antibodies for CFTR studies.
For most expression studies focusing on the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, sensitive and specific antibodies (Abs) are critically needed. Several Abs have been produced commercially or by research laboratories for CFTR detection in both cell lines with heterologous or endogenous expression and native cells/tissues. Here, we review the applicability of most Abs currently in use in CF research for the biochemical and/or immunocytochemical detection of CFTR.
Mendes
Filipa
F
Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Lisboa, Portugal.
Farinha
Carlos M
CM
Roxo-Rosa
Mónica
M
Fanen
Pascale
P
Edelman
Aleksander
A
Dormer
Robert
R
McPherson
Margaret
M
Davidson
Heather
H
Puchelle
Edith
E
De Jonge
Hugo
H
Heda
Ghanshyam D
GD
Gentzsch
Martina
M
Lukacs
Gergely
G
Penque
Deborah
D
Amaral
Margarida D
MD
D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques
D000906:Antibodies; D000911:Antibodies, Monoclonal; C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator