Quantitative analysis of 33 benzodiazepines, metabolites and benzodiazepine-like substances in whole blood by liquid chromatography-(tandem) mass spectrometry.
A quantitative method using high-performance liquid chromatography-mass spectrometry (LC-MS, ion trap) after matrix supported liquid-liquid extraction is described for the simultaneous determination in whole blood of 33 benzodiazepines including metabolites and benzodiazepine-like substances. The limits of detection (LOD) range from 0.0001 to 0.0126 mg/l. Linearity is satisfactory for all compounds. The extraction recoveries for the benzodiazepines in whole blood are between 60 and 91%, desmethyldiazepam, OH-bromazepam and brotizolam excepted. Selectivity, accuracy and precision are satisfactory for clinical and forensic purposes.
Department of Toxicology, Netherlands Forensic Institute, PO Box 3110, 2280 GC Rijswijk, The Netherlands. b.smink@nfi.minjus.nl
B E
BE
Smink
J E
JE
Brandsma
A
A
Dijkhuizen
K J
KJ
Lusthof
J J
JJ
de Gier
A C G
AC
Egberts
D R A
DR
Uges
D001569:Benzodiazepines / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D005554:Forensic Medicine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001569:Benzodiazepines