Augmentation of macrophage phagocytosis by modified arabinoxylan rice bran (MGN-3/biobran).
MGN-3/Biobran, modified arabinoxylan rice bran, has been shown to be a potent biological response modifier (BRM) as manifested by stimulation of different arms of the immune system such as NK, T and B cells; however, its effect on macrophages has not yet been studied. The effects of MGN-3 on macrophage function was examined in vitro using 3 models: human macrophage cell line U937, murine macrophage cell line RAW264.7, and murine peritoneal macrophages (P-M phi). Treatment with MGN-3 resulted in an increase in the percentages of attachment and phagocytosis of yeast by macrophages. The effect depends on the type of macrophage and the dose of MGN-3 applied. Macrophages also demonstrated enhancement in their spreading ability, post treatment with MGN-3. Results also showed that MGN-3, in a dose dependent manner (1, 10,100 microg/ml), significantly induced high levels of production of cytokines: TNF-alpha; and IL-6. In addition, MGN-3 significantly increased nitric oxide (NO) production. This data demonstrates that MGN-3 is a potent inducer of phagocytic function by macrophage, and suggests that MGN-3 is a useful agent for fighting microbial infection.
Department of Otolaryngology, Drew University of Medicine and Science, Los Angeles, CA 90059, USA.
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Ghoneum
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Matsuura
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000276:immunology; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014990:Xylans / Q000494:pharmacology*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014990:Xylans; C419427:polysaccharide MGN3; D009569:Nitric Oxide