Aspects of the early moments of interaction between tachyzoites of Toxoplasma gondii with neutrophils.
The interaction of the tachyzoite stage of Toxoplasma gondii with neutrophils was investigated. Morphological aspects of the initial moments of interaction were analyzed by transmission and scanning electron microscopy, revealing at least three types of reaction from the leukocytes to the parasite: the projection of filopodia, formation of a tunnel-like projection involving the parasite, and invagination of the leukocyte surface at the point of entry. The influence on infectivity of tyrosine kinase, phosphokinase C and phosphatidylinositol 3 kinase cell signaling pathways were studied with the aid of drugs affecting these enzymes in these cells.
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS-Bloco G, Ilha do Fundão, Rio de Janeiro, RJ 21941-900, Brazil.
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MacLaren
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Attias
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de Souza
D000199:Actins / Q000502:physiology; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D047630:Depsipeptides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009281:Naphthalenes / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology / Q000469:parasitology* / Q000648:ultrastructure; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats; D014122:Toxoplasma / Q000469:parasitology / Q000502:physiology* / Q000648:ultrastructure; D014123:Toxoplasmosis / Q000469:parasitology* / Q000473:pathology*; D000077191:Wortmannin
D000199:Actins; D000730:Androstadienes; D047630:Depsipeptides; D004791:Enzyme Inhibitors; D019833:Genistein; D009281:Naphthalenes; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases; D000077191:Wortmannin; C061465:calphostin complex; C057531:jasplakinolide