Complete sequence of a Kyrgyzstan swine hepatitis E virus (HEV) isolated from a piglet thought to be experimentally infected with human HEV.
Hepatitis E virus (HEV) was identified by RT-PCR amplification with degenerate ORF2 primers in the stool of a piglet experimentally inoculated with a stool suspension from a patient with acute hepatitis during an outbreak of non-A, non-B hepatitis in Kyrgyzstan. Further characterization by sequencing of the complete genome and phylogenetic analysis showed that the piglet isolate was most closely related to HEV genotype 3. Because the original human stool specimen used to inoculate the piglet was no longer available, stool samples from three patients obtained during the same outbreak were sequenced and found to be HEV genotype 1. These findings suggest that the HEV isolated from the swine stool was probably an HEV enzootic in Kyrgyzstan and not the virus inoculated from the human stool.
Lu
Ling
L
Laboratory Branch, Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Drobeniuc
Jan
J
Kobylnikov
Nikolay
N
Usmanov
Rafik K
RK
Robertson
Betty H
BH
Favorov
Michael O
MO
Margolis
Harold S
HS
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D016751:Hepatitis E / Q000635:transmission / Q000662:veterinary*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D007707:Kyrgyzstan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
D017931:DNA Primers