The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel.
Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.
Department of Physiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. D.N.Sheppard@bristol.ac.uk
David N
DN
Sheppard
Michael A
MA
Gray
Xiandi
X
Gong
Yoshiro
Y
Sohma
Ilana
I
Kogan
Dale J
DJ
Benos
Toby S
TS
Scott-Ward
Jeng-Haur
JH
Chen
Hongyu
H
Li
Zhiwei
Z
Cai
Jyoti
J
Gupta
Canhui
C
Li
Mohabir
M
Ramjeesingh
Bakhrom K
BK
Berdiev
Iskander I
II
Ismailov
Christine E
CE
Bear
Tzyh-Chang
TC
Hwang
Paul
P
Linsdell
Martin J
MJ
Hug
D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008051:Lipid Bilayers; D018408:Patch-Clamp Techniques / Q000379:methods*
C505032:CFTR protein, human; D008051:Lipid Bilayers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator