Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells.
It has been shown that a chicken anemia virus (CAV) isolates which had undergone 60 passages in MSB-1 cells (SMSC-1/P60, 3-1/P60) acquired 33-66 nucleotide substitutions at the coding region resulting in 13-16 amino acid changes as compared to the CAV isolates passaged only 5 times in MSB-1 cells (SMSC-1 and 3-1) (Chowdhury et al., Arch. Virol. 148, 2437-2448, 2003). In this study we found that a low CAV (BL-5) and a high CAV passage (BL-5/P90) differed by only 15 nucleotide substitutions resulting in 11 amino acid changes. Phylogenetic analysis based on VP1 also revealed that both isolates were close to each other but not to other CAV isolates from Malaysia, namely SMSC-1 and 3-1.
Hasmah
M S
MS
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Omar
A R
AR
Wan
K F
KF
Hair-Bejo
M
M
Aini
I
I
D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D017727:Chicken anemia virus / Q000235:genetics* / Q000254:growth & development*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012692:Serial Passage
D036022:Capsid Proteins; D004279:DNA, Viral; C442548:VP1 protein, Chicken anemia virus; C442521:VP2 protein, Chicken anemia virus; C091717:VP3 protein, Chicken anemia virus