Protective effect of melatonin on 3-nitropropionic acid-induced oxidative stress in synaptosomes in an animal model of Huntington's disease.
The effect of melatonin (1 mg/kg BW i.p./day) on the oxidative changes produced by 3-nitropropionic acid (20 mg/kg BW/day for 4 days) in rat striatal and cortical synaptosomes was investigated. The effects of 3-nitropropionic acid were evaluated as changes in the quantity of lipid peroxidation products, protein carbonyl groups and superoxide dismutase and succinate dehydrogenase activities. 3-Nitropropionic acid caused a rise in lipid peroxidation levels and protein carbonyls content whereas it induced a reduction in the activity of succinate dehydrogenase and triggered an enhancement in superoxide dismutase activity. These changes were prevented by previous administration of melatonin. Our results reveal: (i) 3-nitropropionic acid induces a status of oxidative stress in some brain regions of the Wistar rat; (ii) melatonin prevents the deleterious effects induced by the acid. In conclusion, the results show the ability of melatonin to modify the neural response to 3-nitropropionic acid with the protective mechanism likely involving the antioxidative processes of melatonin.
Department of Biochemistry and Molecular Biology, School of Medicine, Cordoba University, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain.
Isaac
I
Túnez
Pedro
P
Montilla
M
M
Del Carmen Muñoz
Montserrat
M
Feijóo
Manuel
M
Salcedo
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D006816:Huntington Disease / Q000139:chemically induced / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000627:therapeutic use*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D009574:Nitro Compounds; D018384:Oxidative Stress / Q000187:drug effects*; D011422:Propionates / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D000975:Antioxidants; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D011422:Propionates; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase; D008550:Melatonin; C015392:3-nitropropionic acid