Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer.
BACKGROUND
Several cell lines and primary cultures benefit from the use of positively charged extracellular matrix proteins or polymers that enhance their ability to attach to culture plates. Polyethyleneimine is a positively charged polymer that has gained recent attention as a transfection reagent. A less known use of this cationic polymer as an attachment factor was explored with several cell lines.


RESULTS
Polyethyleneimine compared favorably to traditional attachment factors such as collagen and polylysine. PC-12 and HEK-293 cells plated on dishes coated with polyethyleneimine showed a homogeneous distribution of cells in the plate, demonstrating strong cell adhesion that survived washing procedures. The polymer could also be used to enhance the adherence and allow axonal outgrowth from zebrafish retinal explants. The effects of this coating agent on the transfection of loosely attaching cell lines were studied. Pre-coating with polyethyleneimine had the effect of enhancing the transfection yield in procedures using lipofection reagents.


CONCLUSIONS
Polyethyleneimine is an effective attachment factor for weakly anchoring cell lines and primary cells. Its use in lipofection protocols makes the procedures more reliable and increases the yield of expressed products with commonly used cell lines such as PC-12 and HEK-293 cells.
Vancha
Ajith R
AR
Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX 78363, USA. ajith.vancha@jefferson.edu <ajith.vancha@jefferson.edu>
Govindaraju
Suman
S
Parsa
Kishore V L
KV
Jasti
Madhuri
M
González-García
Maribel
M
Ballestero
Rafael P
RP
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D016326:Extracellular Matrix Proteins; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D016716:PC12 Cells / Q000166:cytology / Q000378:metabolism; D011094:Polyethyleneimine / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D051381:Rats; D013499:Surface Properties; D014162:Transfection / Q000379:methods / Q000592:standards
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D008081:Liposomes; D011108:Polymers; D011094:Polyethyleneimine