Stylopine from Chelidonium majus inhibits LPS-induced inflammatory mediators in RAW 264.7 cells.
Stylopine is a major component of the leaf of Chelidonium majus L. (Papaveraceae), which has been used for the removal of warts, papillomas and condylomas, as well as the treatment of liver disease, in oriental countries. Stylopine per se had no cytotoxic effect in unstimulated RAW 264.7 cells, but concentration-dependently reduced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta), and the IL-6 production and cyclooxygenase-2 (COX-2) activity caused by the LPS stimulation. The levels of inducible nitric oxide synthase (iNOS) and COX-2 protein expressions were markedly suppressed by stylopine in a concentration dependent manner. These results suggest that stylopine suppress the NO and PGE2 production in macrophages by inhibiting the iNOS and COX-2 expressions. These biological activities of stylopine may contribute to the anti-inflammatory activity of Chelidonium majus.
Department of Skin & beauty, Seojeong College, Yangju 482-860, Korea.
Seon Il
SI
Jang
Byung Hee
BH
Kim
Woo-Yiel
WY
Lee
Sang Jin
SJ
An
Han Gil
HG
Choi
Byung Hun
BH
Jeon
Hun-Taeg
HT
Chung
Jung-Rae
JR
Rho
Young-Jun
YJ
Kim
Kyu-Yun
KY
Chai
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D028281:Chelidonium; D004305:Dose-Response Relationship, Drug; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D018515:Plant Leaves; D018517:Plant Roots
D000893:Anti-Inflammatory Agents; D001600:Berberine Alkaloids; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C014212:stylopine