[Cryopreserved neurons of a mollusc are capable of morphological differentiation in culture in vitro].
It was shown in culture in vitro that neurons isolated from the cryopreserved brain of adult molluscs Lymnaea stagnalis L. retain viability. Isolated brains were frozen in liquid nitrogen vapors at a rate of 400-500 degrees C/min in the presence of 2 M dimethylsulfoxide. The samples were then plunged into liquid nitrogen and stored from 1 month to 2 years. Upon thawing and removing the cryoprotectant, the neurons were isolated from the brain and then introduced into a cellular culture in vitro. It was shown that the thawed neurons were capable of regenerating new nerve processes similar to those formed by unfrozen neurons in the control.
N A
NA
Ivlicheva
E V
EV
Dmitrieva
M A
MA
Kostenko
E N
EN
Gakhova
D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation; D017952:Ganglia, Invertebrate / Q000166:cytology*; D008195:Lymnaea; D009474:Neurons / Q000166:cytology*; D013997:Time Factors