Proteomics techniques for cystic fibrosis research.
Numerous factors, other than mutations in the CFTR gene, affect the phenotypic variability of cystic fibrosis (CF). With a two-dimensional electrophoresis (2-DE) analysis of total protein expression profiles (proteomics) of CF versus non-CF cells it is possible to obtain an integrative picture of CF cellular alterations. Through this approach, proteins that interact differently with wild type- and mutant-CFTR can also be identified (interactomics). This can provide insight into CF pathophysiology as well as clues for novel therapeutic targets. Additionally, protein profiling can ultimately identify novel disease markers with the potential for a CF diagnosis not based on the analysis of CFTR gene.
Centre of Human Genetics, National Institute of Health, Lisboa, Portugal.
Mónica
M
Roxo-Rosa
Noëlie
N
Davezac
Noura
N
Bensalem
Mainak
M
Majumder
Ghanshyam D
GD
Heda
Andrea
A
Simas
Deborah
D
Penque
Margarida D
MD
Amaral
Gergely L
GL
Lukacs
Aleksander
A
Edelman
D019411:Clinical Laboratory Techniques; D003550:Cystic Fibrosis / Q000235:genetics / Q000503:physiopathology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005821:Genetic Techniques; D006801:Humans; D040901:Proteomics / Q000379:methods*; D012106:Research; D020539:Sequence Analysis, Protein / Q000379:methods*