[Effects of three environmental estrogens on expression of proliferation and apoptosis-associated genes in PEO4 cells].
OBJECTIVE
This study was designed to investigate the molecular mechanisms of proliferation and apoptosis by environmental estrogens (n-4-nonylphenol, NP; Bisphenol A, BisA; Dibutyl Phthalate, DBP) in ovarian cancer PEO4 cells.


METHODS
PEO4 cells were maintained in DMEM medium with 10% neonatal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested and seeded in 6-well culture plates or in 75ml flacks. After various concentration of NP, BisA and DBP treatment for 72h, the cells were harvested and detected mRNA and protein expression of PCNA, bcl-2 and bax by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.


RESULTS
32 x 10(-7) mol/L NP and 32 x 10(-7) mol/L BisA could significantly up-regulate PCNA and bcl-2 mRNA expression and down-regulate the bax mRNA expression, and 32 x 10(-6) mol/L DBP could up-regulate PCNA mRNA expression, but had no effect on bax and bcl-2 mRNA expression. These results were further confirmed by following immunohistochemistry.


CONCLUSIONS
PCNA, bcl-2 and bax pathway might involve in cell proliferation and apoptosis events by environmental estrogens in ovarian cancer PEO4 cells.
School of Public Health, University Zhengzhou, Zhengzhou 450052, China.
Zengli
Z
Yu
Lishi
L
Zhang
Desheng
D
Wu
D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003993:Dibutyl Phthalate / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D010636:Phenols / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003993:Dibutyl Phthalate; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds