Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75.
Sequencing of the gene that encodes the capsid protein VP1 has been used as a surrogate for antigenic typing in order to distinguish enterovirus serotypes; three new serotypes were identified recently by this method. In this study, 14 enterovirus isolates from six countries were characterized as members of two new types within the species Human enterovirus B, based on sequencing of the complete capsid-encoding (P1) region. Isolates within each of these two types differed significantly from one another and from all other known enterovirus serotypes on the basis of sequences that encode either VP1 alone or the entire P1 region. Members of each type were > or =77.2 % identical to one another (89.5 % amino acid identity) in VP1, but members of the two different types differed from one another and from other enteroviruses by > or =31 % in nucleotide sequence (25 % amino acid sequence difference), indicating that the two groups represent separate new candidate enterovirus types. The complete P1 sequences differed from those of all other enterovirus serotypes by > or =31 % (26 % amino acid sequence difference), but were highly conserved within a serotype (<8 % amino acid sequence difference). Phylogenetic analyses demonstrated that isolates of the same serotype were monophyletic in both VP1 and the capsid as a whole, as shown previously for other enterovirus serotypes. This paper proposes that these 14 isolates should be classified as members of two new human enterovirus types, enteroviruses 74 and 75 (EV74 and EV75).
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G-17, Atlanta, GA 30333, USA.
M Steven
MS
Oberste
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G-17, Atlanta, GA 30333, USA.
Suzanne M
SM
Michele
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G-17, Atlanta, GA 30333, USA.
Kaija
K
Maher
Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond, CA, USA.
David
D
Schnurr
Instituto Nacional de Enfermedades Infectiosos - ANLIS 'Carlos Malbran', Buenos Aires, Argentina.
Daniel
D
Cisterna
Department of Virology, Swedish Institute for Disease Control, Solna, Sweden.
Nina
N
Junttila
Institute of Public Health, Dhaka, Bangladesh.
Moyez
M
Uddin
Centre National de Référence des Entérovirus, Lyon, France.
Jean-Jacques
JJ
Chomel
Department of Health, Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China.
Chi-Shan
CS
Lau
National Polio Laboratory, Baghdad, Iraq.
Walid
W
Ridha
Department of Laboratories, Directorate General of Health Affairs, Ministry of Health, Muscat, Oman.
Suleiman
S
Al-Busaidy
Department of Virology, Swedish Institute for Disease Control, Solna, Sweden.
Helene
H
Norder
Department of Virology, Swedish Institute for Disease Control, Solna, Sweden.
Lars O
LO
Magnius
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G-17, Atlanta, GA 30333, USA.
Mark A
MA
Pallansch
D036022:Capsid Proteins / Q000235:genetics; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D036022:Capsid Proteins