Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures.
Thirteen isolates of human respiratory syncytial viruses (HRSV) of groups A and B were isolated in HEp-2 cells from nasopharyngeal aspirates (NPA) from the children with acute respiratory infections. Three isolates of HRSV of group A were propagated in HEp-2 cells in 20 serial passages. Nucleotide sequences of the products obtained by RT-PCR from the glycoprotein (G) hypervariable region of the original virus isolates in NPA and those after one or several passages were compared. All the isolates analyzed showed no changes during passaging in HEp-2 cells.
Sección Virología, Facultad de Ciencias, Universidad de la República, Igua 4225 (11400), Montevideo, Uruguay.
M
M
de Sierra
J
J
Arbiza
D002460:Cell Line; D002675:Child, Preschool; D018388:Codon, Terminator; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D004847:Epithelial Cells / Q000821:virology; D014644:Genetic Variation; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D018357:Respiratory Syncytial Virus Infections / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012692:Serial Passage; D014583:Uruguay; D014759:Viral Envelope Proteins / Q000235:genetics*
D018388:Codon, Terminator; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C439064:glycoprotein O, Human respiratory syncytial virus