Chromatin domain boundaries delimited by a histone-binding protein in yeast.
When located next to chromosomal elements such as telomeres, genes can be subjected to epigenetic silencing. In yeast, this is mediated by the propagation of the SIR proteins from telomeres toward more centromeric regions. Particular transcription factors can protect downstream genes from silencing when tethered between the gene and the telomere, and they may thus act as chromatin domain boundaries. Here we have studied one such transcription factor, CTF-1, that binds directly histone H3. A deletion mutagenesis localized the barrier activity to the CTF-1 histone-binding domain. A saturating point mutagenesis of this domain identified several amino acid substitutions that similarly inhibited the boundary and histone binding activities. Chromatin immunoprecipitation experiments indicated that the barrier protein efficiently prevents the spreading of SIR proteins, and that it separates domains of hypoacetylated and hyperacetylated histones. Together, these results suggest a mechanism by which proteins such as CTF-1 may interact directly with histone H3 to prevent the propagation of a silent chromatin structure, thereby defining boundaries of permissive and silent chromatin domains.
Ferrari
Sélène
S
Institute of Biotechnology, Center for Biotechnology UNIL-EPFL, University of Lausanne, CH-1015 Lausanne, Switzerland.
Simmen
Katia Carmine
KC
Dusserre
Yves
Y
Müller
Karin
K
Fourel
Geneviève
G
Gilson
Eric
E
Mermod
Nicolas
N
D000818:Animals; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D017353:Gene Deletion; D020868:Gene Silencing; D017930:Genes, Reporter; D005822:Genetic Vectors; D006657:Histones / Q000737:chemistry*; D047468:Immunoprecipitation; D051379:Mice; D008954:Models, Biological; D008957:Models, Genetic; D016296:Mutagenesis; D009154:Mutation; D051839:NFI Transcription Factors; D041681:NIH 3T3 Cells; D017354:Point Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016615:Telomere / Q000648:ultrastructure; D014157:Transcription Factors / Q000737:chemistry; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D022762:CCAAT-Enhancer-Binding Proteins; C077305:CTF-1 transcription factor; D002843:Chromatin; D005656:Fungal Proteins; D006657:Histones; D051839:NFI Transcription Factors; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D004247:DNA