The PAK family kinase Cla4 is required for budding and morphogenesis in Ustilago maydis.
The phytopathogenic basidiomycete Ustilago maydis displays a dimorphic switch between budding growth of haploid cells and filamentous growth of the dikaryon. In a screen for mutants affected in morphogenesis and cytokinesis, we identified the serine/threonine protein kinase Cla4, a member of the family of p21-activated kinases (PAKs). Cells, in which cla4 has been deleted, are viable but they are unable to bud properly. Instead, cla4 mutant cells grow as branched septate hyphae and divide by contraction and fission at septal cross walls. Delocalized deposition of chitinous cell wall material along the cell surface is observed in cla4 mutant cells. Deletion of the Cdc42/Rac1 interaction domain (CRIB) results in a constitutive active Cla4 kinase, whose expression is lethal for the cell. cla4 mutant cells are unable to induce pathogenic development in plants and to display filamentous growth in a mating reaction, although they are still able to secrete pheromone and to undergo cell fusion with wild-type cells. We propose that Cla4 is involved in the regulation of cell polarity during budding and filamentation.
Philipps-Universität Marburg, Fachbereich Biologie, 35032 Marburg, Germany.
Leonora
L
Leveleki
Michael
M
Mahlert
Björn
B
Sandrock
Michael
M
Bölker
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D048749:Cytokinesis / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009024:Morphogenesis; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D014588:Ustilago / Q000201:enzymology* / Q000502:physiology* / Q000648:ultrastructure; D003313:Zea mays / Q000382:microbiology
D000199:Actins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C095212:CLA4 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D020558:GTP Phosphohydrolases