Comparative pharmacology of bovine, human and rat vasopressin receptor isoforms.
In this study, we characterized the bovine vasopressin V(1a), V(1b), V(2) receptor isoforms and compared their pharmacological properties to those of corresponding rat and human vasopressin receptor subtypes. Specific binding sites of high affinity for vasopressin were found in all bovine tissues tested (kidney, liver and pituitary). Using a large series of recent peptidic and non-peptidic selective vasopressin agonists or antagonists, we demonstrated the presence of vasopressin V(2), V(1a) or V(1b) receptors in the kidney, liver and pituitary bovine tissues, respectively. This extensive characterization of bovine vasopressin receptor isoforms validates the pharmacological vasopressin receptor classification earlier established for the rat and human species. As expected, the bovine vasopressin receptors look much more like human receptors than rat ones. Interestingly, among the three vasopressin receptor isoforms studied, the vasopressin V(1b) receptor subtype is the best conserved for the three species studied.
INSERM U 469, 141, rue de la Cardonille, 34094 Montpellier Cedex 5, France.
Miriam
M
Andrés
Ana
A
Peña
Sylvain
S
Derick
Danièle
D
Raufaste
Jerzy
J
Trojnar
Kazimierz
K
Wisniewski
Miguel
M
Trueba
Claudine
C
Serradeil-Le Gal
Gilles
G
Guillon
D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006801:Humans; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D020033:Protein Isoforms / Q000819:agonists / Q000378:metabolism; D051381:Rats; D017483:Receptors, Vasopressin / Q000819:agonists* / Q000378:metabolism*; D013045:Species Specificity; D014667:Vasopressins / Q000494:pharmacology
D020033:Protein Isoforms; D017483:Receptors, Vasopressin; D014667:Vasopressins