Polygalacturonic acid/endo-polygalacturonase system: a kinetic study in batch reactors.
The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.
Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università degli Studi di L'Aquila, 67040 Monteluco di Roio (AQ), Italy. gallifuo@ing.univaq.it
Alberto
A
Gallifuoco
Maria
M
Cantarella
Paolo
P
Viparelli
Mariagrazia
M
Marucci
D019149:Bioreactors; D003198:Computer Simulation; D004789:Enzyme Activation; D004795:Enzyme Stability; D007700:Kinetics; D008956:Models, Chemical; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010368:Pectins / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry*; D011485:Protein Binding; D013379:Substrate Specificity
D010368:Pectins; D011096:Polygalacturonase; C003181:polygalacturonic acid