Mimotopes of conformational epitopes in fibrillar beta-amyloid.
In Alzheimer's disease (AD) beta-amyloid peptide accumulates in the brain in different forms including fibrils. Amyloid fibrils could be recognized as foreign by the mature immune system since they are not present during its development. Thus, using mouse antisera raised against the fibrillar form of Abeta42, we have screened two phage peptide libraries for the presence of foreign conformational mimotopes of Abeta. Antisera from wild type animals recognized predominately peptides with the EFRH motif from Abeta42 sequence, whereas amyloid precursor protein (APP) transgenic mice recognized mainly phage clones that mimic epitopes (mimotopes) within the fibrillar Abeta42 but lack sequence homology with this peptide.
Gevorkian
Goar
G
Instituto de investigaciones Biomédicas, Universidad Nacional Autonoma de México, México.
Petrushina
Irina
I
Manoutcharian
Karen
K
Ghochikyan
Anahit
A
Acero
Gonzalo
G
Vasilevko
Vitaly
V
Cribbs
David H
DH
Agadjanyan
Michael G
MG
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000939:Epitopes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018716:Molecular Mimicry / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding / Q000235:genetics; D011487:Protein Conformation
D016229:Amyloid beta-Peptides; D000939:Epitopes; D010446:Peptide Fragments; D019151:Peptide Library; C075222:amyloid beta-protein (1-42)