Effect of exercise on hepatic gene expression in rats: a microarray analysis.
Exercise has various beneficial effects on liver function, enhancing both nutrient metabolism and antioxidant capacity. To explore the molecular mechanisms underlying these changes, we used a high-density cDNA microarray containing probe sets for 2,845 genes to analyze changes of gene transcription in the livers of rats after 4 weeks of running exercise. In comparison with sedentary animals, 105 genes were up-regulated and 86 genes were down-regulated, including genes with unknown functions. In addition, we detected an increase of p38 mitogen-activated protein kinase and protein and of the protein for signal transducer and activator of transcription 3 (stat3), corresponding to the increase of these mRNAs shown by microarray analysis. These results indicate that long-term exercise can alter liver function via changes of gene expression, especially the genes encoding signal transduction proteins such as p38 and stat3.
Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. waoi@koto.kpu-m.ac.jp
Wataru
W
Aoi
Eiichiro
E
Ichiishi
Naoyuki
N
Sakamoto
Atsumi
A
Tsujimoto
Harukuni
H
Tokuda
Toshikazu
T
Yoshikawa
D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000502:physiology; D015534:Trans-Activators; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism / Q000502:physiology
D017931:DNA Primers; D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators; D048051:p38 Mitogen-Activated Protein Kinases