Effects of simulated microgravity on the development and maturation of dissociated cortical neurons.
Although a wealth of evidence supports the hypothesis that some functions of the nervous system may be altered during exposure to microgravity, the possible changes in basic neuronal physiology are not easy to assess. Indeed, few studies have examined whether microgravity affects the development of neurons in culture. In the present study, a suspension of dissociated cortical cells from rat embryos were exposed to 24 h of simulated microgravity before plating in a normal adherent culture system. Both preexposed and control cells were used after a period of 7-10 d in vitro. The vitality and the level of reactive oxygen species of cultures previously exposed did not differ from those of normal cultures. Cellular characterization by immunostaining with a specific antibody displayed normal neuronal phenotype in control cells, whereas pretreatment in simulated microgravity revealed an increase of glial fibrillary acidic protein fluorescence in the elongated stellate glial cells. Electrophysiological recording indicated that the electrical properties of neurons preexposed were comparable with those of controls. Overall, our results indicate that a short time of simulated microgravity preexposure does not affect dramatically the ability of dissociated neural cells to develop and differentiate in an adherent culture system.
Crestini
Alessio
A
Department of Cellular Biology and Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Zona
Cristina
C
Sebastiani
Pierluigi
P
Pieri
Massimo
M
Caracciolo
Valentina
V
Malvezzi-Campeggi
Lorenzo
L
Confaloni
Annamaria
A
Di Loreto
Silvia
S
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D004594:Electrophysiology / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D018474:Weightlessness Simulation
D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D017382:Reactive Oxygen Species