Serial analysis of gene expression in sinusoidal endothelial cells from normal and injured mouse liver.
Here we describe gene expression profiles of mouse liver sinusoidal endothelial cells (LSECs) revealed by serial analysis of gene expression (SAGE). We prepared SAGE libraries of LSECs from normal and injured liver by CCl(4) administration, and we obtained 32,867 tags from normal and 37,493 tags from injured liver, representing 6011 unique transcripts. CCl(4) administration upregulated several genes related to cell growth and differentiation (Cdkn1a, Irf1, Il4ra, etc.), whereas it downregulated genes related to cell growth or protein transport (Kdr, Igfbp4, Ap1b1, etc.). To identify genes preferentially expressed in LSEC, we compared our SAGE libraries with 77 publicly available libraries generated from various mouse tissues and cell lines. We identified 23 genes, including Stab2 and uncharacterized genes, as possible markers for LSEC, which will be useful to analyze the specific role for LSECs in normal as well as regenerating liver.
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Hidenori
H
Nonaka
Sumio
S
Sugano
Atsushi
A
Miyajima
D000818:Animals; D015415:Biomarkers; D002251:Carbon Tetrachloride / Q000633:toxicity; D048708:Cell Growth Processes; D002469:Cell Separation; D030561:Databases, Nucleic Acid; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis
D015415:Biomarkers; D002251:Carbon Tetrachloride