[Inflammatory injurious effect of angiotensin II on pulmonary microvascular endothelium in rat].
OBJECTIVE
To investigate the effects of angiotensin II (AngII) and its receptors on monolayer permeability of pulmonary microvascular endothelial cells in rat.


METHODS
The following examinations were done on cultured rat pulmonary microvascular endothelial cells (RPMVECs). 1. Micro-filter was used to assay the variations of lipopolysaccharide (LPS)-induced increased RPMVECs monolayer permeability coefficient (Kf) in different periods. 2. Effect of angiotensin II on LPS-induced permeability injury to the endothelium, and preventive effects of angiotensin II type 1 receptor antagonist (Sar1, Ile8)-Ang II.


RESULTS
LPS increased RPMVECs monolayer permeability compared with normal control, and AngII exacerbated LPS-induced RPMVECs monolayer permeability significantly. This synergistic effect was significantly prevented by the addition of (Sar1, Ile8 )-Ang II.


CONCLUSIONS
AngII and LPS have synergistic injurious effects to the pulmonary microvascular endothelium in rat, and AngII exacerbates increase in LPS-induced RPMVECs monolayer permeability. This synergistic effect is significantly prevented by the addition of (Sar1, Ile8)-Ang II.
Department of Emergency, First Affiliated Hospital, Anhui Medical University, Hefei 230061, Anhui, China.
Hong
H
Zhang
Geng-Yun
GY
Sun
D000804:Angiotensin II / Q000494:pharmacology*; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D004347:Drug Interactions; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000098:blood supply; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D047228:Angiotensin II Type 1 Receptor Blockers; D008070:Lipopolysaccharides; D000804:Angiotensin II