The melanocortin receptor subtypes in chicken have high preference to ACTH-derived peptides.
1 Melanocortin (MC) receptors are widely distributed throughout the body of chicken, like in mammals, and participate in a wide range of physiological functions. 2 To clarify the pharmacological impact of ligands acting in the MC system, we expressed the chicken MC1, MC2, MC3, MC4 and MC5 (cMC1-5) receptors in eukaryotic cells and performed comprehensive pharmacological characterization of the potency of endogenous and synthetic melanocortin peptides. 3 Remarkably, the cMC receptors displayed high affinity for ACTH-derived peptides and in general low affinity for alpha-MSH. It is evident that not only the cMC2 receptor but also the other cMC receptors interact with ACTH-derived peptide through an epitope beyond the sequence of alpha-MSH. 4 The synthetic ligand MTII was found to be a potent agonist whereas HS024 was a potent antagonist at the cMC4 receptor, indicating that these ligands are suitable for physiological studies in chicken. 5 We also show the presence of prohormone convertase 1 (PC1) and PC2 genes in chicken, and that these peptides are coexpressed with proopiomelanocortin (POMC) in various tissues.
Department of Neuroscience, Uppsala University, BMC, Box 593, 751 24 Uppsala, Sweden.
Maria K
MK
Ling
Eri
E
Hotta
Zuzana
Z
Kilianova
Tatjana
T
Haitina
Aneta
A
Ringholm
Lisa
L
Johansson
Nicole
N
Gallo-Payet
Sakae
S
Takeuchi
Helgi B
HB
Schiöth
D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002460:Cell Line; D002645:Chickens / Q000378:metabolism*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D010802:Phylogeny; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D045664:Proprotein Convertase 1 / Q000235:genetics / Q000378:metabolism; D045706:Proprotein Convertase 2 / Q000235:genetics / Q000378:metabolism; D011869:Radioligand Assay; D044101:Receptors, Melanocortin / Q000096:biosynthesis / Q000187:drug effects* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014162:Transfection; D000521:alpha-MSH / Q000494:pharmacology
D010455:Peptides; D044101:Receptors, Melanocortin; D000521:alpha-MSH; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D045664:Proprotein Convertase 1; D045706:Proprotein Convertase 2