Preparation and characterisation of poly(vinyl alcohol)/cyclodextrin microspheres as matrix for inclusion and separation of drugs.
Poly(vinyl alcohol) (PVA) microspheres containing cyclodextrin (CD) were obtained by chemical cross-linking with glutaraldehyde of an acidified mixture solution of PVA and alpha-, beta- or gamma-CD. The amount of linked CD in microspheres, estimated by tetrazolium blue method, decreases in the order beta- > gamma- > alpha-CD. The dimensions of PVA/gamma-CD microspheres are much higher than those of PVA/alpha- and beta-CD. The cross-linking density of microspheres was estimated by the amount of iodine retained by the polymer matrix. The pore size as well as the porous volume of PVA/CD microspheres decrease significantly on increasing the amount of glutaraldehyde, but are enough large to permit the access of drugs to the CD cavity. In order to test the PVA/CD microsphere inclusion ability, the microspheres were packed in a glass column and the liquid chromatographic behaviour by isocratic elution of different drugs or typical organic compounds, taken as model drugs, was investigated.
Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara, 19 44100 Ferrara, Italy.
Marieta
M
Constantin
Gheorghe
G
Fundueanu
Fabrizio
F
Bortolotti
Rita
R
Cortesi
Paolo
P
Ascenzi
Enea
E
Menegatti
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003505:Cyclodextrins / Q000138:chemical synthesis*; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D005976:Glutaral / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D014677:Pharmaceutical Vehicles / Q000737:chemistry / Q000493:pharmacokinetics; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis*; D016062:Porosity; D013678:Technology, Pharmaceutical / Q000379:methods
D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D004337:Drug Carriers; D014677:Pharmaceutical Vehicles; D011142:Polyvinyl Alcohol; D005976:Glutaral