Indirect covariance NMR spectroscopy.
A novel NMR scheme is presented that establishes homonuclear spin correlations without requiring direct detection of the spin species. This covariance NMR method is experimentally demonstrated for a mixture of amino acids and for the uniformly 13C-labeled cyclic decapeptide antamanide using a 13C-edited TOCSY experiment. The method opens up new avenues for the experimental analysis of molecules containing insensitive spins encountered in biomolecular NMR and analytical chemistry including metabolomics.
Carlson School of Chemistry and Biochemistry, Clark University, Worcester, Massachusetts 01610, USA.
Fengli
F
Zhang
Rafael
R
Brüschweiler
D005583:Fourier Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010456:Peptides, Cyclic / Q000737:chemistry
D010456:Peptides, Cyclic; C100265:antamanide