Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations.
Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.
Mellmann
Alexander
A
Institut für Hygiene, Universitätsklinikum Münster, Münster, Germany.
Mosters
Jan
J
Bartelt
Edda
E
Roggentin
Peter
P
Ammon
Andrea
A
Friedrich
Alexander W
AW
Karch
Helge
H
Harmsen
Dag
D
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D002417:Cattle; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005408:Flagellin / Q000235:genetics*; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA
D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin