[Strategy for the identification of amplified genes in tumors].
Amplification of genomic DNA is often observed in tumors. The identification of genes in amplified regions may be helpful to the discovery of oncogenes associated with a specific tumor. Array-based comparative genomic hybridization and restriction landmark genomic scanning are applicable to the definition of such regions. The copy number alterations of target genes present in these regions and the levels of their mRNA and protein can be characterized by quantitative PCR and tissue microarray staining with immunohistochemistry. Transfection, RNA interference, cDNA microarray or reverse transcription-multiplex ligation-dependent probe amplification will facilitate demonstrating the role of such amplified genes in the pathogenesis of a specific tumor.
National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100021 P.R. China.
Man-li
ML
Luo
Ming-rong
MR
Wang
D005784:Gene Amplification / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection