Hyalinizing trabecular adenoma of the thyroid gland identification through MIB-1 staining of fine-needle aspiration biopsy smears.
Smears of fine-needle aspiration (FNA) biopsy specimens of 24 histologically proven and MIB-1-positive hyalinizing trabecular adenomas stained with Papanicolaou or Diff-Quik were stripped from the original slides, transferred to charged slides, destained, and restained with MIB-1. Positive immunoreactivity with MIB-1 in a peripheral cytoplasmic pattern was present in 17 specimens, no immunoreactivity was seen in 4 specimens, and equivocal staining was seen in 3 specimens. Papanicolaou-stained smears of papillary thyroid carcinoma, medullary thyroid carcinoma, thyroid follicular neoplasm, and Hürthle cell adenoma (5 specimens each) were treated similarly. None of the control cases stained with MIB-1 had the peripheral cytoplasmic pattern. Hyalinizing trabecular adenoma can be distinguished reliably from other thyroid neoplasms by MIB-1 staining of destained cells from FNA biopsy specimens.
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Mary B
MB
Casey
Thomas J
TJ
Sebo
J Aidan
JA
Carney
D000236:Adenoma / Q000175:diagnosis / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D008875:Middle Aged; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000473:pathology*
D019394:Ki-67 Antigen