Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library.
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC). The initial peptide substrate screening for Hsp40 Ydj1 has been carried out by utilizing a 7-mer phage display library. The peptide sequences from the bio-panning were synthesized and object to the direct affinity measurement for Hsp40 Ydj1 by isothemol titration calirimetry studies. The peptide which has the measurable affinity with Ydj1 shows enriched hydrophobic residues in the middle of the substrate fragment. The peptide substrate specificity for molecular chaperone Hsp40 has been analyzed.
Department of Cel Biology, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham. MCLM 364, 1918 Univ. Blvd., University of Alabama at Birmingham, Birmingham, AL 35294-0005. USA.
Jingzhi
J
Li
Bingdong
B
Sha