Distribution of heat shock protein 108 mRNA during the development of the chicken brain.
The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108+) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).
Shin
Dong Hoon
DH
Department of Anatomy, MRC Neuroscience Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
Lee
Eunju
E
Kim
Jaehyup
J
Bae
Su-ryeon
SR
Chung
Yoon Hee
YH
Kim
Hyun Joon
HJ
Lee
Mun Han
MH
Cho
Sa Sun
SS
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization / Q000379:methods; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D033863:Transferrin-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D033863:Transferrin-Binding Proteins; D020932:Nerve Growth Factor