[Evaluation of the inmunohistochemical markers PCNA and MIB-1 in the diagnosis of low grade lesions of the uterine cervix].
A prospective and comparative study of 112 uterine cervix biopsies, received from hospitals of Maracaibo, Venezuela, with the diagnosis of cervical intraepithelial neoplasia I (CIN I) determined with the traditional Hematoxilin-Eosin (H-E) diagnostic staining, was performed to compare the inmunohistochemical markers PCNA and MIB-1 with the conventional H-E staining. The samples were classified with H-E, and 3 samples were excluded of the study with a different diagnostic from CIN I. After the inmunohistochemical study, 19 biopsies (17.4%) were classified as CIN II with PCNA and 18 (16.5%) with MIB-1. The analysis showed a significant difference (p < 0.01) between the H-E staining and the inmunomarkers. These results allow us to conclude that the inmunohistochemical evaluation of the proliferative activity is very useful to establish with certainty the diagnosis of preneoplasie lesions of uterine cervix and also represents and important parameter in the prognostic evaluation.
Postgrado de Anatomía Patológica, Escuela de Medicina, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela. mvsanchez@luz.ve
Milagros
M
Sánchez-Rosales
Sergio
S
Osorio-Morales
Felipe
F
Díaz-Araujo
D001706:Biopsy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D011446:Prospective Studies; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000473:pathology*
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen