[A study on inflammatory regulation of human gallbladder epithelial cells by PPAR-gamma ligand].
OBJECTIVE
To investigate the inflammatory regulation of human gallbladder epithelial cells (HGBEC) by peroxisome proliferator activated receptor gamma (PPAR-gamma) ligand ciglitazone.


METHODS
HGBEC were cultured in medium containing human epidermal growth factor (hEGF). HIL-1beta were added into the ciglitazone groups and inflammatory control groups to make inflammatory model . IL-6 and TNF-alpha concentration in ciglitazone groups and all control groups were measured.


RESULTS
HGBEC were cultured in medium successfully. The inflammatory model was made. The longest duration is 25 d. In inflammatory control groups, cells were edema with unclear cellular membrane and plasmid. In ciglitazone groups, the inflammatory edema of cells were less evident than that in inflammatory control groups, especially in 50 micromol/ml group. The IL-6 concentration in ciglitazone groups is lower than that in control group (P<0.01). The relation between the inhibitory effect and the concentration of ciglitazone is positive correlation.


CONCLUSIONS
Ciglitazone that can inhibit the inflammation of HGBEC maybe an effective treatment for acute and chronic cholecystitis.
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Guang-dong
GD
Pan
Nan-sheng
NS
Cheng
Xian-ze
XZ
Xiong
Jiang-wen
JW
Liu
Sheng-fu
SF
Li
Guo-fu
GF
Zahng
Lü-nan
LN
Yan
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D005704:Gallbladder / Q000166:cytology*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D008024:Ligands; D047495:PPAR gamma / Q000037:antagonists & inhibitors*; D045162:Thiazolidinediones / Q000494:pharmacology*; D014157:Transcription Factors / Q000819:agonists / Q000378:metabolism / Q000502:physiology
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D015850:Interleukin-6; D008024:Ligands; D047495:PPAR gamma; D045162:Thiazolidinediones; D014157:Transcription Factors; C039671:ciglitazone