Differential regulation of variant glucocorticoid receptor mRNAs in the rat hippocampus by the antidepressant fluoxetine.
Adult rats were given antidepressant drugs orally. Fluoxetine, but not moclobemide, venlafaxine, tianeptine or desipramine, increased total glucocorticoid receptor (GR) mRNA in the hippocampus after 4 weeks. Further examination revealed that GR mRNA containing the brain-specific exon 1(7) was increased across all hippocampal subregions. In contrast, expression of the major exon 1(10) and another brain-specific exon 1(5)-containing GR mRNAs were unchanged. Tissue-specific first exon usage may contribute to the differential regulation of GR by fluoxetine in brain subregions.
Endocrinology Unit, Molecular Medicine Centre, Western General Hospital, Edinburgh EH4 2XU, Scotland, UK. Joyce.Yau@ed.ac.uk
Joyce L W
JL
Yau
June
J
Noble
Karen E
KE
Chapman
Jonathan R
JR
Seckl
D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D005091:Exons; D005473:Fluoxetine / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*
D018687:Antidepressive Agents, Second-Generation; D020033:Protein Isoforms; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D005473:Fluoxetine