Biochemical methods to assess CFTR expression and membrane localization.
Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.
Centre of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal. cfarinha@igc.gulbenkian.pt
Carlos M
CM
Farinha
Deborah
D
Penque
Mónica
M
Roxo-Rosa
Gergely
G
Lukacs
Robert
R
Dormer
Margaret
M
McPherson
Malcolm
M
Pereira
Alice G M
AG
Bot
Huub
H
Jorna
Rob
R
Willemsen
Hugo
H
Dejonge
Ghanshyam D
GD
Heda
Christopher R
CR
Marino
Pascale
P
Fanen
Alexandre
A
Hinzpeter
Joanna
J
Lipecka
Janine
J
Fritsch
Martina
M
Gentzsch
Aleksander
A
Edelman
Margarida D
MD
Amaral
D019921:Biotinylation; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000302:isolation & purification*; D005821:Genetic Techniques; D006031:Glycosylation; D006801:Humans; D007158:Immunologic Techniques
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator