CD200 receptor family members represent novel DAP12-associated activating receptors on basophils and mast cells.
Modulation by balancing activating and inhibitory receptors constitutes an important mechanism for regulating lymphocyte and myeloid cell effector responses. Using a microarray screen during parasitic helminth infection, we identified CD200 receptor-like 3 as a transcript highly expressed in basophils. Novel splice variants were present that generated proteins that differed in surface expression. The second immunoglobulin-like domain, encoded by exon 4, was required for cell surface expression and recruitment of DAP12 to the cell surface. Splice variants also generated unique cytoplasmic domains, which contributed to efficient pairing with DAP12. Despite expression on basophils and mast cells, which are integral components of allergic immunity, the absence of DAP12 did not alter effector cell recruitment or the host response elicited by helminth infection with Nippostrongylus brasiliensis.
Department of Medicine, University of California San Francisco, CA 94143-0654, USA.
David
D
Voehringer
David B
DB
Rosen
Lewis L
LL
Lanier
Richard M
RM
Locksley
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001491:Basophils / Q000737:chemistry* / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D008407:Mast Cells / Q000737:chemistry* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009559:Nippostrongylus; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017206:Strongylida Infections / Q000276:immunology; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; C492346:CD200 receptor, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C484963:Tyrobp protein, mouse