Effect of exercise on hepatic gene expression in rats: a microarray analysis.
Exercise has various beneficial effects on liver function, enhancing both nutrient metabolism and antioxidant capacity. To explore the molecular mechanisms underlying these changes, we used a high-density cDNA microarray containing probe sets for 2,845 genes to analyze changes of gene transcription in the livers of rats after 4 weeks of running exercise. In comparison with sedentary animals, 105 genes were up-regulated and 86 genes were down-regulated, including genes with unknown functions. In addition, we detected an increase of p38 mitogen-activated protein kinase and protein and of the protein for signal transducer and activator of transcription 3 (stat3), corresponding to the increase of these mRNAs shown by microarray analysis. These results indicate that long-term exercise can alter liver function via changes of gene expression, especially the genes encoding signal transduction proteins such as p38 and stat3.
Aoi
Wataru
W
Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. waoi@koto.kpu-m.ac.jp
Ichiishi
Eiichiro
E
Sakamoto
Naoyuki
N
Tsujimoto
Atsumi
A
Tokuda
Harukuni
H
Yoshikawa
Toshikazu
T
D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000502:physiology; D015534:Trans-Activators; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism / Q000502:physiology
D017931:DNA Primers; D004268:DNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators; D048051:p38 Mitogen-Activated Protein Kinases