[Changes in lipid asymmetry and transport of glutathione conjugates under the influence of calcium ions in human erythrocytes].
The influence of calcium ions on the distribution of the fluorescent analog of phosphatidylcholine 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine (C6-NBD-PC) in membranes and transport of glutathione-S-conjugates in human erythrocytes was studied. It was supposed that both processes were performed by the multidrug resistance protein. It was found that the increase in intracellular calcium concentration tended to both the redistribution of about 35% of C6-NBD-PC in the inner layer of the membrane and a decrease in the exit of glutathione-S-conjugates from erythrocytes. In both cases, the intracellular calcium concentration varied in the physiological (nanomolar) range. The results testify that Ca2+ participates in the regulation of the activity of the multidrug resistance protein.
Kostin
D G
DG
Kozlova
N M
NM
Slobozhanina
E I
EI
D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D005978:Glutathione; D002118:Calcium