Effects of UV-B radiation on the Patagonian Jaborosa magellanica Brisben.
Treatment of Jaborosa magellanica with artificial UV-B radiation caused changes in plant growth, plant chemistry and increase DNA polymorphisms. Spectrophotometric analysis showed that UV-B radiation decreases the chlorophylls content, and increases the amount of UV-B absorbing compounds (e.g., phenylpropanoids). Other UV-induced alterations include reduction in leaf area, alterations in plant architecture, and DNA damage. Using random primers and PCR amplification procedure, a high degree of polymorphism was detected when treated plants were compared to non-irradiated plants. These biochemical changes may be interpreted as plant response to UV-B radiation stress and as an indicator of DNA damage.
Cuadra
Pedro
P
Centro de Estudios del Cuaternario, Universidad de Magallanes, Casilla 113-D, Punta Arenas, Chile. pedro.cuadra@umag.cl
Herrera
Raúl
R
Fajardo
Víctor
V
D001483:Base Sequence; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D004249:DNA Damage / Q000528:radiation effects; D017931:DNA Primers / Q000235:genetics; D018384:Oxidative Stress / Q000528:radiation effects; D010666:Phenylpropionates / Q000032:analysis / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays
D017931:DNA Primers; D010666:Phenylpropionates; D002734:Chlorophyll