Spatial dispersal of porcine reproductive and respiratory syndrome virus-contaminated flies after contact with experimentally infected pigs.
OBJECTIVE
To determine whether flies can acquire porcine reproductive and respiratory syndrome virus (PRRSV) and disperse the virus throughout a designated area.


METHODS
60 four-month-old pigs.


METHODS
On day 0, 28 of 60 pigs were inoculated with PRRSV MN 30-100 (index variant). On the same day, 100,000 pupae of ochre-eyed houseflies and 100,000 pupae of red-eyed (wild-type) houseflies were placed in the swine facility for a release-recapture study. Flies were recaptured at 2 locations within the swine facility, 6 locations immediately outside the facility, and 30 locations 0.4, 0.8, 1.3, 1.7, 1.9, and 2.3 km from the facility. Traps were emptied on days 2, 7, 8, 10, and 14. Samples derived from flies were tested by use of a polymerase chain reaction assay, virus DNA was sequenced, and viruses were tested for infectivity by means of a swine bioassay.


RESULTS
PRRSV RNA homologous to the index PRRSV was detected in trapped flies collected inside and immediately outside the facility and from 9 of 48 samples collected at 0.4 km, 8 of 24 samples collected at 0.8 km, 5 of 24 samples collected at 1.3 km, and 3 of 84 samples collected at > 1.7 km from the facility. Two samples collected at 0.8 km contained genetically diverse variants of PRRSV. Swine bioassays revealed the virus in flies was infectious.


CONCLUSIONS
Flies appeared to become contaminated with PRRSV from infected pigs and transported the virus > or = 1.7 km. Fly-born transmission may explain how PRRSV is seasonally transported between farms.
Schurrer
Jennifer A
JA
Swine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.
Dee
Scott A
SA
Moon
Roger D
RD
Rossow
Kurt D
KD
Mahlum
Carrie
C
Mondaca
Enrique
E
Otake
Satoshi
S
Fano
Eduardo
E
Collins
James E
JE
Pijoan
Carlos
C
D000818:Animals; D002353:Carrier State / Q000635:transmission; D003710:Demography; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006793:Houseflies / Q000502:physiology / Q000821:virology*; D007303:Insect Vectors / Q000821:virology*; D016013:Likelihood Functions; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000635:transmission*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA / Q000662:veterinary; D034421:Sus scrofa; D013553:Swine Diseases / Q000635:transmission / Q000821:virology*