Antibiotic resistance may affect alkali decontamination of specimens containing mycobacteria.
The influence of genetic modification on the decontamination process of mycobacteria was investigated using a wild type Mycobacterium bovis BCG Pasteur and its isogenic mutant strain. The result showed that the killing effect of sodium hydroxide (NaOH) progressively increased with prolonged incubation (up to 20 minutes), and the deteriorating effect of NaOH was more severe on the genetically modified strain.
Department of Infection, Immunity, and Inflammation, Leicester Medical School, University of Leicester, United Kingdom. hy3@le.ac.uk
Hasan
H
Yesilkaya
Michael R
MR
Barer
Peter W
PW
Andrew
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D003666:Decontamination; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009163:Mycobacterium bovis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D012972:Sodium Hydroxide