Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model.
The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model.
Department of Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Jan
J
Gutenwik
Bernt
B
Nilsson
Anders
A
Axelsson
D000327:Adsorption; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel / Q000379:methods*; D004058:Diffusion; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D018613:Microscopy, Confocal; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D012685:Sepharose / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D007074:Immunoglobulin G; D011506:Proteins; D012710:Serum Albumin, Bovine; D012685:Sepharose; D009113:Muramidase