Bacterial cellulose production by fed-batch fermentation in molasses medium.
Batch and fed-batch fermentations for bacterial cellulose (BC) production using molasses as a carbon source by Acetobacter xylinum BPR2001 were carried out in a jar fermentor. For improvement of BC production, molasses was subjected to H2SO4-heat treatment. The maximum BC concentration by this treated molasses increased 76%, and the specific growth rate increased 2-fold compared with that by untreated molasses. In batch fermentation, when the initial sugar concentrations of H2SO4-heat-treated molasses were varied from 20 to 70 g/L, the highest value of maximum BC concentration of 5.3 g/L was observed at 20 g/L. BC production in intermittent fed-batch (IFB) fermentation was conducted referring to the data in batch fermentation, and the highest BC production of 7.82 g/L was obtained when 0.2 L of molasses medium was added five times. When continuous fed-batch (CFB) fermentations were conducted, maximum BC concentration was obtained with a feeding rate of 6.3 g-sugar/h, which was derived from the optimal IFB experiment.
Chemical Resources Laboratory, Tokyo Institute of Technology, R1-29-4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
Sangok
S
Bae
Makoto
M
Shoda
D019149:Bioreactors / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000096:biosynthesis*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D008965:Molasses / Q000382:microbiology*
D003470:Culture Media; D002482:Cellulose