Rapid characterization of recombinant lambdagt11 clones expressing parasite antigens.
Recombinant DNA techniques allow any parasite gene to be isolated, propagated and analysed in great detail. The techniques are now well known and widely used in research on parasite diagnosis, vaccine development and identification of chemotherapeutic targets. There are difficulties, for example in analysing carbohydrate antigens, and in selecting which genes merit the intensive investigation required. In this article john Kelly and Mark Blaxter discuss two simple procedures that allow recombinant clones to be characterized rapidly on the basis of both the parasite DNA sequences that they carry and the parasite antigens that they produce. They take examples from work on antigens of Leishmania donovani.
Wolfson Unit, Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
J M
JM
Kelly
M L
ML
Blaxter