Genotypic analysis of strains of mutans streptococci by pulsed-field gel electrophoresis.
The species and serotypes of various strains of S. mutans and S. sobrinus were characterized by pulsed-field gel electrophoresis after the genomic DNA from the various strains had been digested with five restriction enzymes (EcoR I, Xba I, Hind III, Sfi I and BssH II) separately. Among these restriction enzymes, BssH II was very useful for the characterization of species and serotypes and, in particular, digestion discriminated between serotypes d and g. The restriction patterns obtained from the genomic DNA of isolates isolated from children's saliva were essentially identical to those from the genomic DNA of the standard laboratory strains. Patterns of BssH II digests of the genomic DNA of 10 isolates identified as S. sobrinus were characteristic of serotype g of the standard laboratory strains. Our results indicate that digestion with BssH II and subsequence analysis by pulsed-field gel electrophoresis should be useful for the characterization of species and serotypes and for epidemiological studies of mutans streptococci.
Department of Oral Microbiology, School of Dentistry at Niigata, Nippon Dental University, 1-8 Hamauracho, Niigata, 951-8580 Japan. rmine@ecatvhome.ne.jp
R
R
Mineyama
S
S
Yoshino
K
K
Fukushima
D000293:Adolescent; D015373:Bacterial Typing Techniques; D002648:Child; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D015183:Restriction Mapping; D012463:Saliva / Q000382:microbiology*; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus / Q000145:classification* / Q000235:genetics*; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics*
C044171:endodeoxyribonuclease BSSHII; D015252:Deoxyribonucleases, Type II Site-Specific