Aminooxyacetic acid inhibits antheridiogenesis and development of Anemia phyllitidis gametophytes.
Cytomorphological studies of the development of young fern gametophytes (Anemia phyllitidis) have been used to investigate combined effects of gibberellic acid and ethylene on male sex expression. ACC (the key by-product in ethylene biosynthesis pathway) was found to exert a synergetic effect on the gibberellic acid-induced antheridia formation, and this phenomenon could be related with the specific stimulation of cell growth and activity of their differentiation. To complete and verify those observations male sex expression in the fern gametophytes treated with ACC-biosynthesis inhibitor was reinvestigated. Aminooxyacetic acid (AOA) restrained antheridia formation via inhibition of cell divisions. AOA influenced the arrangement and flexibility of cellulose microfibrils in the antheridial zone cells, thus affecting cell expansion. On the other hand, the level of DNA synthesis was not reduced. Transient increase in the number of S-phase cells, followed by the accumulation of G2-phase cells led to the enhancement of cell polyploidization. All these findings correspond with the previous observations and support participation of ethylene in gibberellic acid-induced male sex expression in ferns.
Department of Cytophysiology, University of Łódź, Pilarskiego 14, 90231, Poland. kazand@biol.uni.lodz.pl
Andrzej
A
Kaźmierczak
D000598:Amino Acids, Cyclic / Q000494:pharmacology; D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D018744:DNA, Plant / Q000096:biosynthesis / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005030:Ethylenes / Q000096:biosynthesis / Q000494:pharmacology; D029624:Ferns / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D035264:Flowers / Q000187:drug effects* / Q000254:growth & development; D005718:Gametogenesis / Q000187:drug effects* / Q000502:physiology; D005854:Germ Cells / Q000187:drug effects* / Q000254:growth & development; D005875:Gibberellins / Q000494:pharmacology
D000598:Amino Acids, Cyclic; D018744:DNA, Plant; D004791:Enzyme Inhibitors; D005030:Ethylenes; D005875:Gibberellins; D000625:Aminooxyacetic Acid; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; C007842:gibberellic acid