Detection of Helicobacter pylori in bile of cats.
Lymphocytic cholangitis (LC) in cats is a biliary disease of unknown etiology. Helicobacter spp. were recently implicated in human primary sclerosing cholangitis (PSC) and primary biliary cirrhosis (PBC). Because of the similarities between PSC/PBC with LC, we hypothesized that Helicobacter spp. are involved in feline LC. A PCR with Helicobacter genus-specific 16S rRNA primers was performed on DNA isolated from feline bile samples. Four of the 15 (26%) LC samples were positive, whereas only 8/51 (16%) of non-LC samples were PCR positive (p=0.44). Sequence analysis of the amplicons revealed a 100% identity with the Helicobacter pylori specific DNA fragments. Our data suggest an etiological role of H. pylori in feline LC and that cats are a potential zoonotic reservoir.
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, P.O. Box 80154, 3508 TD Utrecht, The Netherlands. s.y.boomkens@vet.uu.nl
Sacha Y
SY
Boomkens
Johannes G
JG
Kusters
Gaby
G
Hoffmann
Raymond G J
RG
Pot
Bart
B
Spee
Louis C
LC
Penning
Herman F
HF
Egberink
Ted S G A M
TS
van den Ingh
Jan
J
Rothuizen
D000818:Animals; D001646:Bile / Q000382:microbiology*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D002761:Cholangitis / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D016481:Helicobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S