[Effects on DNA damage and apoptosis and p53 protein expression induced by fluoride in human embryo hepatocytes].
OBJECTIVE
To study the effects of DNA damage and apoptosis and p53 expression, and to explore the relationship between apoptosis and p53 expression in human embryo hepatocytes induced by fluoride.


METHODS
The rate of DNA damage, apoptosis and the level of p53 expression were investigated after the cells were incubated with sodium fluoride for about 24 hours. The concentrations of sodium fluoride of the control, A, B and C group were 0 microg/ml, 40 microg/ ml, 80 microg/ml and 160 microg/ml individually.


RESULTS
The rate of DNA damage of every group treated with sodium fluoride were significantly higher than the control group (P < 0.05). The percentage of apoptosis of B and C groups increased apparently (P < 0.05). The level of p53 expression of B and C groups were significantly higher than the control group (P < 0.01).


CONCLUSIONS
Fluoride can increase the rate of DNA damage, and induce apoptosis and expression of p53 in human embryo hepatocytes. Furthermore, both apoptosis and the level of p53 expression, there exists a rise tendency with the increase concentration of fluoride.
Ha
Jianli
J
Hubei Vocational-Technical College, Xiaogan 432000, China.
Chu
Qilong
Q
Wang
Aiguo
A
Xia
Tao
T
Yang
Kedi
K
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000196:embryology; D012969:Sodium Fluoride / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53; D012969:Sodium Fluoride