Multilocus variable-number tandem-repeat polymorphism among Brazilian Enterococcus faecalis strains.
Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative to PFGE.
Molecular Microbiology and Biotechnology, University of Brasília, Brasilia-DF, Brazil. titze@unb.br
Ricardo
R
Titze-de-Almeida
Rob J L
RJ
Willems
Janetta
J
Top
Isabela Pereira
IP
Rodrigues
Renato Fonseca
RF
Ferreira
Hélène
H
Boelens
Maria Christina C
MC
Brandileone
Rosemeire C
RC
Zanella
Maria Sueli Soares
MS
Felipe
Alex
A
van Belkum
D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D016521:Electrophoresis, Gel, Pulsed-Field; D013293:Enterococcus faecalis / Q000145:classification* / Q000235:genetics; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic