Cross-species amplification of Bovidae microsatellites and low diversity of the endangered Korean goral.
The Korean goral (Nemorhaedus caudatus) is an endangered species of wild goat. The conservation and management of this species could benefit from a better understanding of its genetic diversity and structure. Cross-species amplification of 34 Bovidae microsatellite loci was tested on a panel of 6 Korean gorals and 10 domestic goats. After polymerase chain reaction (PCR) optimization, 29 (85.3%) microsatellite loci amplified successfully for the Korean gorals and 27 (79.4%) for the domestic goats. Of the amplified products, 16 (55.2%) were polymorphic in the Korean goral and 22 (81.5%) in domestic goats. Nei's unbiased mean heterozygosity and mean allele number per locus were, respectively, 0.356 and 2.6 in the Korean goral and 0.636 and 4.8 in domestic goats. Low genetic diversity in the Korean gorals observed in this preliminary microsatellite survey suggests an urgent need for further detailed study of genetic diversity in Korean goral populations and a population management strategy based on these studies. Current results of cross-species amplification of domestic Bovidae microsatellites could be employed for the necessary population genetic studies on the Korean goral and other endangered Caprinae species.
Kim
K-S
KS
Conservation Genome Resource Bank for Korean Wildlife, Research Institute of Veterinary Science, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. kkim@iastate.edu
Min
M-S
MS
An
J-H
JH
Lee
H
H
D000818:Animals; D000835:Animals, Wild; D002417:Cattle; D003247:Conservation of Natural Resources; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012418:Ruminants / Q000235:genetics*; D012756:Sheep
D005819:Genetic Markers