Mechanism of membrane binding of the phospholipase D1 PX domain.
Mammalian phospholipases D (PLD), which catalyze the hydrolysis of phosphatidylcholine to phosphatidic acid (PA), have been implicated in various cell signaling and vesicle trafficking processes. Mammalian PLD1 contains two different membrane-targeting domains, pleckstrin homology and Phox homology (PX) domains, but the precise roles of these domains in the membrane binding and activation of PLD1 are still unclear. To elucidate the role of the PX domain in PLD1 activation, we constructed a structural model of the PX domain by homology modeling and measured the membrane binding of this domain and selected mutants by surface plasmon resonance analysis. The PLD1 PX domain was found to have high phosphoinositide specificity, i.e. phosphatidylinositol 3,4,5-trisphosphate (PtdIns-(3,4,5)P(3)) >> phosphatidylinositol 3-phosphate > phosphatidylinositol 5-phosphate >> other phosphoinositides. The PtdIns(3,4,5)P(3) binding was facilitated by the cationic residues (Lys(119), Lys(121), and Arg(179)) in the putative binding pocket. Consistent with the model structure that suggests the presence of a second lipid-binding pocket, vesicle binding studies indicated that the PLD1 PX domain could also bind with moderate affinity to PA, phosphatidylserine, and other anionic lipids, which were mediated by a cluster of cationic residues in the secondary binding site. Simultaneous occupancy of both binding pockets synergistically increases membrane affinity of the PX domain. Electrostatic potential calculations suggest that a highly positive potential near the secondary binding site may facilitate the initial adsorption of the domain to the anionic membrane, which is followed by the binding of PtdIns(3,4,5)P(3) to its binding pocket. Collectively, our results suggest that the interaction of the PLD1 PX domain with PtdIns(3,4,5)P(3) and/or PA (or phosphatidylserine) may be an important factor in the spatiotemporal regulation and activation of PLD1.
Stahelin
Robert V
RV
Department of Chemistry, University of Illinois at Chicago, Illinois 60607. USA.
Ananthanarayanan
Bharath
B
Blatner
Nichole R
NR
Singh
Shaneen
S
Bruzik
Karol S
KS
Murray
Diana
D
Cho
Wonhwa
W
D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions; D001120:Arginine; D001665:Binding Sites; D002412:Cations; D002462:Cell Membrane / Q000201:enzymology*; D004789:Enzyme Activation; D008239:Lysine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010739:Phospholipase D / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D016415:Sequence Alignment; D055672:Static Electricity; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance
D000838:Anions; D002412:Cations; D010446:Peptide Fragments; D010712:Phosphatidic Acids; D018129:Phosphatidylinositol Phosphates; D010718:Phosphatidylserines; C060974:phosphatidylinositol 3,4,5-triphosphate; D001120:Arginine; D010739:Phospholipase D; C106801:phospholipase D1; D008239:Lysine