Early embryo-endometrial signaling modulates the regulation of matrix metalloproteinase-3.
OBJECTIVE
To examine matrix metalloproteinase-3 (MMP-3) expression in human stromal cell culture after P stimulation and the effect of conditioned medium from human embryo-epithelial cells coculture on its expression and activity.


METHODS
Metabolic and endocrine studies on human tissue.


METHODS
In vitro fertilization (i.v.f.) unit and endocrine research unit.


METHODS
Infertile patients undergoing endometrial tissue sampling for dating at the luteal phase before i.v.f.


METHODS
Endometrial sampling and collection of human embryos culture media.


METHODS
Expression and activity of secreted MMP-3 by P-induced stromal cells, and in stromal cells exposed to conditioned medium from embryo-epithelial cell coculture.


RESULTS
Expression and activity of MMP-3 in human stromal cells decrease after P induction. Following incubation of these stromal-derived decidual cells with conditioned medium from embryo-epithelial cell coculture, MMP-3 expression and activity increased in a statistically significant manner.


CONCLUSIONS
Progesterone inhibition of MMP-3 expression and its support of endometrial integrity were prevented by local expression of MMP-3 in response to embryonic signaling.
Department of Obstetrics and Gynecology, IVF Unit, Carmel Medical Center, Haifa, Israel. lahav@adi.org il
Shirly
S
Lahav-Baratz
Hanna
H
Shiloh
Mara
M
Koifman
Zaki
Z
Kraiem
Zofnat
Z
Wiener-Megnazi
David
D
Ishai
Martha
M
Dirnfeld
D000328:Adult; D001755:Blastocyst / Q000201:enzymology; D002454:Cell Differentiation; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D046508:Culture Techniques; D003656:Decidua / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development; D004717:Endometrium / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D009028:Morula / Q000201:enzymology; D011374:Progesterone / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology; D013997:Time Factors
D017077:Culture Media, Conditioned; D061965:Matrix Metalloproteinase Inhibitors; D011374:Progesterone; D019278:Matrix Metalloproteinase 3