On scrapie interference and artificial prions.
The mechanisms responsible for neuronal death in transmissible spongiform encephalopathies (TSEs) are still not completely understood, and at least two major hypotheses have been formulated, based on the peculiar aspects of prion protein biology. In fact, the neuronal spreading of the prion conformational change may lead either to gain toxic properties, or to loose the normal function of this protein. In order to investigate the relative contribution of these two opposite mechanisms, two theoretical approaches may be proposed: RNA interference (RNAi) and artificial prion engineering. In fact, RNAi techniques offer now an extremely exciting new tool for investigating the effects of gene silencing both in prion, and other neurological disorders. On the other hand, the gain-of-toxic-function hypothesis might be definitely evaluated by creating an artificial prion choosing a protein target whose loss of function could be bypassed in the experimental set. In this paper the two aforementioned strategies are outlined, briefly discussing the consequent implications for TSE therapy.
Tremolizzo
Lucio
L
Laboratory of Neurobiology, D.N.T.B., University of Milano-Bicocca, S.Gerardo Hospital, Villa Serena 4 p. Sud, via Donizetti 106, 20052 Monza (MI), Italy. lucio.tremolizzo@unimib.it
Rodriguez-Menendez
Virginia
V
Ferrarese
Carlo
C
D000818:Animals; D020868:Gene Silencing; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D011328:Prions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012107:Research Design; D012608:Scrapie / Q000235:genetics* / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011328:Prions; D011994:Recombinant Proteins