Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry.
OBJECTIVE
To investigate the antibacterial effect of five essential oils (EO) extracted from aromatic plants (Cymbopogon citratus, Ocimumbasilicum, Ocimum gratissimum, Thymus vulgaris and Zingiber officinale) of Cameroon against strains of Listeria monocytogenes, L. innocua and Staphylococcus aureus. The ability of selected EO to permeabilize the cytoplasmic membrane of L. innocua was also examined.


RESULTS
The antibacterial activity of the EO determined by the agar diffusion method showed that T. vulgaris had the highest activity followed by O. gratissimum and C. citratus. Lowest activity was recorded from Z. officinale and O. basilicum. Significant differences in sensitivity between strains of Listeria and S. aureus were observed. Flow cytometry of L. innocua stained with carboxy-fluorescein diacetate showed that the fluorescence intensity of cells exposed to EO decreased faster than nonexposed cells, indicating that EO permeabilized the cytoplasmic membrane with the leakage of carboxy-fluorescein.


CONCLUSIONS
Almost all the EO tested showed antibacterial activity to a different extent. The antibacterial effect was due to permeabilization of the cytoplasmic membrane.


CONCLUSIONS
This study has identified the preservative potential of the EO examined. The use of sensitive method, such as flow cytometry, is advantageous for quick generation of data on the antibacterial effect of EO.
Department of Biochemistry, University of Yaounde-I, Box 812, Yaounde, Cameroon. jnguefack2001@yahoo.co.uk
J
J
Nguefack
B B
BB
Budde
M
M
Jakobsen
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002163:Cameroon; D002463:Cell Membrane Permeability / Q000187:drug effects*; D031743:Cymbopogon / Q000737:chemistry; D005434:Flow Cytometry; D005452:Fluoresceins / Q000032:analysis; D020939:Zingiber officinale / Q000737:chemistry; D008087:Listeria / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D031339:Ocimum / Q000737:chemistry; D018649:Ocimum basilicum / Q000737:chemistry; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology; D013194:Staining and Labeling; D013211:Staphylococcus aureus / Q000187:drug effects; D046930:Thymus Plant / Q000737:chemistry
D000900:Anti-Bacterial Agents; D005452:Fluoresceins; D009822:Oils, Volatile; D010938:Plant Oils; C024098:6-carboxyfluorescein