Preparation and characterisation of poly(vinyl alcohol)/cyclodextrin microspheres as matrix for inclusion and separation of drugs.
Poly(vinyl alcohol) (PVA) microspheres containing cyclodextrin (CD) were obtained by chemical cross-linking with glutaraldehyde of an acidified mixture solution of PVA and alpha-, beta- or gamma-CD. The amount of linked CD in microspheres, estimated by tetrazolium blue method, decreases in the order beta- > gamma- > alpha-CD. The dimensions of PVA/gamma-CD microspheres are much higher than those of PVA/alpha- and beta-CD. The cross-linking density of microspheres was estimated by the amount of iodine retained by the polymer matrix. The pore size as well as the porous volume of PVA/CD microspheres decrease significantly on increasing the amount of glutaraldehyde, but are enough large to permit the access of drugs to the CD cavity. In order to test the PVA/CD microsphere inclusion ability, the microspheres were packed in a glass column and the liquid chromatographic behaviour by isocratic elution of different drugs or typical organic compounds, taken as model drugs, was investigated.
Constantin
Marieta
M
Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara, 19 44100 Ferrara, Italy.
Fundueanu
Gheorghe
G
Bortolotti
Fabrizio
F
Cortesi
Rita
R
Ascenzi
Paolo
P
Menegatti
Enea
E
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003505:Cyclodextrins / Q000138:chemical synthesis*; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D005976:Glutaral / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D014677:Pharmaceutical Vehicles / Q000737:chemistry / Q000493:pharmacokinetics; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis*; D016062:Porosity; D013678:Technology, Pharmaceutical / Q000379:methods
D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D004337:Drug Carriers; D014677:Pharmaceutical Vehicles; D011142:Polyvinyl Alcohol; D005976:Glutaral