"Microcanals" for micropipette access to single cells in microfluidic environments.
We demonstrate the fabrication and operation of "microcanals"(i.e. open-air microfluidic channels without a roof), which enable micropipette manipulation and probing of cells within a microfluidic environment. The microcanal devices are fabricated in PDMS on glass substrates using a PDMS membrane transferring technique. Here we show patch-clamp electrophysiological recording and intracellular dye injection performed on cells seeded in microcanals.
Hsu
Chia-Hsien
CH
Department of Bioengineering, University of Washington, Seattle, WA 98105, USA.
Chen
Chihchen
C
Folch
Albert
A
D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D005898:Glass / Q000737:chemistry; D006801:Humans; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation*; D041681:NIH 3T3 Cells; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods*; D012828:Silicones / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D012828:Silicones