Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil.
In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.
Godoy
Patricio
P
Disciplina de Doenças Infecciosas e Parasitárias (DIPA), Universidade Federal de São Paulo, Brazil.
Cano
Josep
J
Gené
Josepa
J
Guarro
Josep
J
Höfling-Lima
Ana Luisa
AL
Lopes Colombo
Arnaldo
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D001938:Brazil; D015821:Eye Infections, Fungal / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005670:Fusarium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D015994:Incidence; D007634:Keratitis / Q000453:epidemiology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length