Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase.
Protein palmitoyltransferases (PATs) represent an exciting new target for anticancer drug design due to their pivotal roles in the subcellular localization of a number of oncogenes. We show that the Huntingtin interacting protein 14 (HIP14) is a PAT with a preference for the farnesyl-dependent palmitoylation motif found in H- and N-RAS. Characterization of HIP14 in mouse cells has revealed that it has the ability to induce colony formation in cell culture, anchorage-independent growth, and tumors in mice. Activity of the enzyme and its ability to transform cells is dependent on critical residues in the active site of the enzyme.
Ducker
Charles E
CE
Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive Hershey, PA 17033, USA.
Stettler
Erin M
EM
French
Kevin J
KJ
Upson
John J
JJ
Smith
Charles D
CD
D000217:Acyltransferases; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000502:physiology*; D002471:Cell Transformation, Neoplastic; D017931:DNA Primers; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D041681:NIH 3T3 Cells; D009419:Nerve Tissue Proteins / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D000217:Acyltransferases; C473038:ZDHHC17 protein, human