[Expression of nerve growth factor mRNA and nerve regeneration after discontinuous injection of phorbol-12-myristate-13-acetate into silicone chamber].
OBJECTIVE
To study the mRNA expressions of protein kinase C(PKC) and nerve growth factor (NGF) in rat sciatic nerve and the number of axons after phorbol-12-myristate-13-acetate (PMA) was injected into silicone chamber.


METHODS
Forty-two SD adult rats were divided into six groups depending on the time of injury (1 day, 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks). A 0.5 cm nerve was cut in double rat sciatic nerves and "T" type silicone chamber was sutured. PMA at the concentration of 1 x 10(-9) mol/L was injected discontinuously into the right side of T type silicone chamber (PMA group) and saline was injected into the left side (control group). Nucleic acid in situ hybridization histochemistry technique and the computer imagine analysis were employed to detect dynamic changes of PKC mRNA and NGF mRNA in rat sciatic nerves. The number of axons was measured.


RESULTS
The expressions of PKC mRNA and NGF mRNA increased after injury, and the expressions of PKC mRNA and NGF mRNA reached the peak 2 weeks and 3 weeks after injury respectively in control group. The expressions of PKC mRNA and NGF mRNA in PMA group were significantly increased than those in control group 2,3 and 4 weeks after injury (P < 0.01). The number of axons in PMA group significantly increased than that in control group (P < 0.01).


CONCLUSIONS
PKC involved in the expression of NGF mRNA and nerve regeneration after injury. During the regenerated course, PMA can promote the expression of NGF mRNA and the number of axons after injury.
Department of Orthopedic Surgery, Henan Provincial Hospital, Zhengzhou Henan 450003, PR China.
Y
Y
Jin
Yongxiang
Y
Luo
Jia
J
Zheng
D000818:Animals; D020536:Enzyme Activators / Q000494:pharmacology; D008297:Male; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D009416:Nerve Regeneration / Q000187:drug effects*; D011493:Protein Kinase C / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology; D012828:Silicones; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology*
D020536:Enzyme Activators; D012333:RNA, Messenger; D012828:Silicones; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; D020932:Nerve Growth Factor; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate