Human liver specific transcriptional factor TCP10L binds to MAD4.
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, P.R. China.
Dao-Jun
DJ
Jiang
Hong-Xiu
HX
Yu
Sa-Yin
SY
Hexige
Ze-Kun
ZK
Guo
Xiang
X
Wang
Li-Jie
LJ
Ma
Zheng
Z
Chen
Shou-Yuan
SY
Zhao
Long
L
Yu
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002460:Cell Line; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D056427:t-Complex Genome Region
D051792:Basic Helix-Loop-Helix Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C484347:MXD4 protein, human