Secretion mode and subcellular localization of human interferon-beta exogenously expressed in porcine renal epithelial LLC-PK1 cells.
We have investigated the secretion polarity of human interferon-beta (HuIFN-beta) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-beta was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-beta was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-beta revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-beta is regulated by a post trans-Golgi network in a cell type-dependent manner.
Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Japan.
Teruko
T
Nishio
Masato
M
Maruyama
Toyokazu
T
Yoshida
Yoshihiko
Y
Watanabe
Makiya
M
Nishikawa
Yoshinobu
Y
Takakura
D000818:Animals; D016764:Cell Polarity; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D018374:LLC-PK1 Cells; D021381:Protein Transport; D013552:Swine; D014162:Transfection; D021601:trans-Golgi Network / Q000502:physiology
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D016899:Interferon-beta