Comparative analysis of the proteome of left ventricular heart of arteriosclerosis in rat.
Despite the worldwide occurrence of coronary atherosclerotic heart disease (CAHD), the pathogenic mechanisms underlying this disease remain largely unknown. In this study, the experimental model of atherosclerosis in rat (CAHD rat) was established by the injection of vitamin D3 associated with high fat diet for 6 weeks. By using the proteomic approach, we comparatively analyzed the proteome of the control and CAHD rat left ventricular myocardial tissues. We reproducibly separated over 2500 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-11. Among these proteins, 26 proteins with large amount were identified using micro high performance liquid chromatography mass spectrometer/mass spectrometer (micro-HPLC-MS/MS). Using PDQUEST software to process the 2-DE gel images, 38 protein spots that significantly altered in CAHD were detected. Of these, 12 proteins were identified with high confidence by using 2-DE and matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The identification of protein alterations specify to CAHD would clarify the pathogenetic mechanisms involved in the disease and might be of prognostic and therapeutic benefit.
Department of Chemistry, Fudan University, 220 HanDan Road, Shanghai 200433, China.
Zhen-Yu
ZY
Huang
Peng-Yuan
PY
Yang
Mohamad Radwan
MR
Almofti
Yan-Ling
YL
Yu
Yao-Cheng
YC
Rui
Peng-Yuan
PY
Yang
D000818:Animals; D001011:Aorta / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D003324:Coronary Artery Disease / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003331:Coronary Vessels / Q000473:pathology; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006352:Heart Ventricles / Q000378:metabolism*; D006652:Histological Techniques; D008297:Male; D013058:Mass Spectrometry; D009206:Myocardium / Q000378:metabolism*; D020543:Proteome; D051381:Rats; D017208:Rats, Wistar; D014807:Vitamin D / Q000633:toxicity
D020543:Proteome; D014807:Vitamin D