Cell lysis on a microfluidic CD (compact disc).
Cell lysis was demonstrated on a microfluidic CD (Compact Disc) platform. In this purely mechanical lysis method, spherical particles (beads) in a lysis chamber microfabricated in a CD, cause disruption of mammalian (CHO-K1), bacterial (Escherichia coli), and yeast (Saccharomyces cerevisiae) cells. Interactions between beads and cells are generated in the rimming flow established inside a partially filled annular chamber in the CD rotating around a horizontal axis. To maximize bead-cell interactions in the lysis chamber, the CD was spun forward and backwards around this axis, using high acceleration for 5 to 7 min. Investigation on inter-particle forces (friction and collision) identified the following parameters; bead density, angular velocity, acceleration rate, and solid volume fraction as having the most significant contribution to cell lysis. Cell disruption efficiency was verified either through direct microscopic viewing or measurement of the DNA concentration after cell lysing. Lysis efficiency relative to a conventional lysis protocol was approximately 65%. In the long term, this work is geared towards CD based sample-to-answer nucleic acid analysis which will include cell lysis, DNA purification, DNA amplification, and DNA hybridization detection.
Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA.
Jitae
J
Kim
Seh
S
Hee Jang
Guangyao
G
Jia
Jim V
JV
Zoval
Nancy A
NA
Da Silva
Marc J
MJ
Madou
D000818:Animals; D016466:CHO Cells; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004926:Escherichia coli / Q000166:cytology; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D011076:Polycarboxylate Cement / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology
D004129:Dimethylpolysiloxanes; D011076:Polycarboxylate Cement; C005506:polycarbonate