Characterisation of a serine proteinase from Penaeus vannamei haemocytes.
Serine proteinases are involved, besides digestive role, in immune response processes. In addition to the typical serine proteinase domain, proteinases from arthropod haemocytes contain so-called clip domains which are believed to exert regulatory functions. Clones coding for clip domain-containing serine proteinases were isolated from both Penaeus vannamei and Penaeus monodon haemocyte cDNA libraries. These proteins have most of the structural characteristics of serine proteinase domain, but in the clip domain there are only four cysteines, whereas in most other clip domains there are six. Such structures are named pseudo-clip domains and apparently seem to be widely distributed in Penaeid shrimp. These proteinases were only expressed in haemocytes and not in muscles, hypodermis, heart, tail stalk, pleopods or hepatopancreas.
Jiménez-Vega
Florinda
F
CIBNOR, PO Box 128, La Paz, BCS 23000, Mexico.
Vargas-Albores
Francisco
F
Söderhäll
Kenneth
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D015723:Gene Library; D006434:Hemocytes / Q000737:chemistry*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
D017931:DNA Primers; D012697:Serine Endopeptidases