Reactivation of human herpesvirus 6 during ex vivo expansion of circulating CD34+ haematopoietic stem cells.
Human herpesvirus 6 (HHV-6) replication was evaluated during in vitro expansion of CD34-positive cells that were selected from 11 peripheral blood progenitor cell (PBPC) samples. In order to permit cellular differentiation towards the myeloid lineage, PBPCs were cultured for 14-21 days in a liquid, serum-free medium supplemented with interleukin 1 (IL1), IL3, IL6, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor and stem-cell factor. Among the 10 cultures from HHV-6-seropositive patients, the late, alternatively spliced U100 viral mRNA was detected in five of them after PBPC culture for 14 or 21 days. Recovery of infectious virus from one of the expansions, associated with an increase of HHV-6 viral load and detection of the U100 spliced messenger, confirmed the occurrence of a complete replicative cycle. These data thus demonstrate for the first time that haematopoietic differentiation can lead to HHV-6 reactivation.
Virology Laboratory, UPRES-JE 2437, Nantes University Hospital and UFR Sciences Pharmaceutiques, 9 quai Moncousu, 44093 Nantes Cedex, France.
Elisabeth
E
Andre-Garnier
Haematology Department, Nantes University Hospital, France.
Noel
N
Milpied
Virology Laboratory, UPRES-EA2387, Pitie-Salpetriere, Paris University Hospital, France.
David
D
Boutolleau
Cell and Gene Therapy Unit, Nantes University Hospital, France.
Soraya
S
Saiagh
Virology Laboratory, UPRES-JE 2437, Nantes University Hospital and UFR Sciences Pharmaceutiques, 9 quai Moncousu, 44093 Nantes Cedex, France.
Sylviane
S
Billaudel
Virology Laboratory, UPRES-JE 2437, Nantes University Hospital and UFR Sciences Pharmaceutiques, 9 quai Moncousu, 44093 Nantes Cedex, France.
Berthe-Marie
BM
Imbert-Marcille
D018952:Antigens, CD34 / Q000032:analysis; D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D019349:Roseolovirus Infections / Q000821:virology*; D019562:Viral Load; D014775:Virus Activation
D018952:Antigens, CD34; D012333:RNA, Messenger; D012367:RNA, Viral