A reverse genetic screen in Drosophila using a deletion-inducing mutagen.
We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.
Nairz
Knud
K
Zoologisches Institut, Universität Zürich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland. nairz@zool.unizh.ch
Zipperlen
Peder
P
Dearolf
Charles
C
Basler
Konrad
K
Hafen
Ernst
E
D000818:Animals; D004252:DNA Mutational Analysis; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006492:Hempa / Q000494:pharmacology; D008297:Male; D016296:Mutagenesis / Q000187:drug effects* / Q000235:genetics*; D009153:Mutagens / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics*
D009153:Mutagens; D006492:Hempa