Proteomic approach to identify acute phase response-related proteins with low molecular weight in loach skin following injury.
Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from the proteomic study. Fifteen APR-related proteins with sixteen spots (PLA with two spots) on a 2-DE map were identified in this study. Furthermore, six were known acute phase proteins including galactose-binding lectin (GBL), lysozyme, C3, CD59, double PLA and 50s ribosomal protein; while ATP kinase, zinc finger protein 183, alpha-neurotoxin homology, angiostatin, serine/threonine kinase, metalloproteinase inhibitor, regulator of G-protein 4, cryptdin-9 and disintegrin trigranin were found by our lab to be APR-related proteins. In addition, our results suggest that proteomes with low molecular weight can be characterized by 2-DE with a Tris-tricine system followed by mass spectrometry.
Peng
Xuanxian
X
Center for Proteomics and Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, PR China. wangpeng@xmu.edu.cn
Wu
Yajun
Y
Chen
Jingan
J
Wang
Sanying
S
D000209:Acute-Phase Proteins / Q000737:chemistry*; D000595:Amino Acid Sequence; D043168:Angiostatins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D002852:Chromatography, Ion Exchange; D019295:Computational Biology / Q000379:methods; D003531:Cypriniformes / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic; D040901:Proteomics / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000209:Acute-Phase Proteins; D043168:Angiostatins; D005998:Glycine; C100184:tricine