[Activation of ERK1/2 and Elk1 in A549 cells induced by crocidolite].
OBJECTIVE
To investigate the role of extracellular signal-regulated kinase (ERK) 1/2 and Elk1 in pulmonary disease induced by crocidolite asbestos fiber.


METHODS
Western blotting and Immunoprecipitation were used to detect the expression of phosphorylated ERK1/2 and Elk1 in human bronchial airway A549 cell line stimulated by crocidolite.


RESULTS
The expression of phosphorylated ERK1/2 and Elk1 were striking higher than those of the control, the differences were significant (P < 0.05).


CONCLUSIONS
Phosphorylated ERK1/2 and Elk1 probably involved in the process of the diseases induced by crocidolite.
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Xinchao
X
Wang
Yiming
Y
Wu
Zhuowei
Z
Li
James M
JM
Samet
Andrew J
AJ
Ghio
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D017638:Asbestos, Crocidolite / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D019950:Mitogen-Activated Protein Kinase 1 / Q000502:physiology*; D048052:Mitogen-Activated Protein Kinase 3 / Q000502:physiology*; D010766:Phosphorylation; D050807:ets-Domain Protein Elk-1 / Q000502:physiology*
C494112:ELK1 protein, human; D050807:ets-Domain Protein Elk-1; D017638:Asbestos, Crocidolite; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3