Increased adjuvant activity of minimal CD8 T cell peptides incorporated into lipid-core-peptides.
A problem facing the use of subunit peptide and protein vaccines is their inability to stimulate protective immune responses. Many different approaches have been utilized to overcome this inefficient immune activation. The approach we have taken is to modify the vaccine antigen so that it now has adjuvant properties. To do this, multiple copies of minimal CD8 T cell epitopes were attached to a poly lysine lipid core. These constructs are known as lipid-core-peptides (LCP). The research presented here examines the adjuvant activity of LCP. Using mouse models, we were able to show that LCP were indeed able to activate antigen-presenting cells in vitro and to activate cytotoxic T-cell responses in vivo. More importantly, LCP were able to stimulate the development of a protective antitumour immune response.
White
Karen
K
School of Pharmacy, University of Otago, Dunedin, New Zealand.
Kearns
Philip
P
Toth
Istvan
I
Hook
Sarah
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000138:chemical synthesis*; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000737:chemistry* / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000008:administration & dosage / Q000276:immunology; D008074:Lipoproteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000517:prevention & control / Q000628:therapy; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology; D011107:Polylysine
D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D008074:Lipoproteins; C110366:OVA-8; D010446:Peptide Fragments; D011107:Polylysine; D010047:Ovalbumin