Inhibitory effect of Aspergillus fumigatus extract on matrix metalloproteinases expression.
BACKGROUND
Matrix metalloproteinases (MMPs) play a role in several physiologic and pathologic events. There are some evidence indicating the involvement of MMPs in the pathophysiology of fungal infections.


METHODS
Here we study somatic extract of Aspergillus fumigatus. The influence of Aspergillus vs. two other fungal extracts on MMPs production by Fibrosarcoma cell line was investigated using an in vitro cytotoxicity assay, sodium dodecyl sulfate-polyacrylamide and gelatin zymography.


RESULTS
Comparative dose-dependent inhibitory effects on MMPs were seen by A. fumigatus extract and compared with some steroidal and non-steroidal drugs. Cytotoxicity analysis of our extract revealed much lower cell death than other examined agents.


CONCLUSIONS
Since inhibition of MMPs activity has been employed in modality therapy in such diseases as cancer, this extract might be promising in the preparation of anti-MMP therapeutic derivatives.
Division of Biotechnology, Department of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, P.O. Box: 14155-6446, Tehran, Iran.
Farshid
F
Saadat
Kamiar
K
Zomorodian
Mohammad
M
Pezeshki
Sassan
S
Rezaie
Mohammad Reza
MR
Khorramizadeh
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000737:chemistry*; D045744:Cell Line, Tumor; D003907:Dexamethasone / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005354:Fibrosarcoma / Q000201:enzymology; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000096:biosynthesis; D010894:Piroxicam / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D010894:Piroxicam; D004008:Diclofenac; D003907:Dexamethasone; D020782:Matrix Metalloproteinases