Strategy for generating tissue-engineered human bone construct.
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
Tan
K K
KK
Department of Orthopaedics & Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Tan
G H
GH
Sabarul Afian
M
M
Ng
M H Angela
MH
Fauziah
O
O
Ruszymah
B H I
BH
D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation; D002455:Cell Division / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D009924:Organ Culture Techniques; D010521:Periosteum / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
D003094:Collagen