Preparation of a tritiated ginkgolide.
Ginkgolide B, a constituent of the tree Ginkgo biloba, was radiolabeled with the beta-emitter tritium ([(3)H]) in two steps from ginkgolide C. First, a triflate precursor was prepared utilizing the selective reactivity of 7-OH in ginkgolide C; the triflate was then reduced with sodium borotritide to yield tritiated ginkgolide B ([(3)H]GB) in good yield and high specific activity. The tritiated ginkgolide will be an important tool for studying neuromodulatory properties of ginkgolides.
Strømgaard
Kristian
K
Department of Chemistry, Columbia University, New York, NY 10027, USA.
Suehiro
Makiko
M
Nakanishi
Koji
K
D004224:Diterpenes / Q000138:chemical synthesis* / Q000737:chemistry; D020441:Ginkgo biloba / Q000737:chemistry; D046934:Ginkgolides; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D008968:Molecular Conformation; D014316:Tritium / Q000737:chemistry*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D014316:Tritium; C058295:ginkgolide C; C045856:ginkgolide B