On the influence of different surfaces in nano- and submicrometer particle based fluorescence immunoassays.
Recently, numerous attempts have been made to improve the performance of fluorescence immunoassays. One way pursued is the substitution of labeling molecules by micro- or nanocrystalline dyes. The surfaces of these particulate structures are typically engineered by a layerwise assembly of oppositely charged polyelectrolytes, the outer layer being constituted of biorecognition molecules, for example, immunoglobulins. In this study, we show that amphiphilic polymers such as alkylated poly(ethylene imine)s and 1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-[amino(poly(ethylene glycol))] can fully substitute the more intricate layer-by-layer technique and evaluate the influence of surface charge and particle size on the overall performance of these assays.
8sens.biognosticAG, Robert-Roessle-Strasse 10, D-13125 Berlin, Germany. Y.Bruemmel@biognostic.de
Yvonne
Y
Bruemmel
Cangel Pui-Yee
CP
Chan
Reinhard
R
Renneberg
Andreas
A
Thuenemann
Matthias
M
Seydack
D005026:Ethylene Glycols / Q000737:chemistry*; D005453:Fluorescence; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D007097:Imines / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011095:Polyethylenes / Q000737:chemistry*; D013499:Surface Properties
C509678:1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-(amino(poly(ethylene glycol))); D005026:Ethylene Glycols; D007097:Imines; D011095:Polyethylenes; C505405:poly(ethylene imine); D005990:Glycerol