Impact of genetically modified Enterobacter cloacae on indigenous endophytic community of Citrus sinensis seedlings.
Enterobacter cloacae (strain PR2/7), a genetically modified endophyte (GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.
Andreote
Fernando Dini
FD
Escola Superior de Agricultura "Luiz de Queiroz"-USP-Departamento de Genética Laboratório de Genética de Microrganismos, Piracicaba-SP, Brazil.
Gullo
Marcelo José Mortatti
MJ
de Souza Lima
André Oliveira
AO
Júnior
Walter Maccheroni
WM
Azevedo
João Lúcio
JL
Araújo
Welington Luiz
WL
D001419:Bacteria / Q000254:growth & development*; D032084:Citrus sinensis / Q000382:microbiology*; D017753:Ecosystem; D016972:Enterobacter cloacae / Q000235:genetics / Q000254:growth & development*; D030781:Organisms, Genetically Modified; D010957:Plasmids; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology*; D014169:Transformation, Bacterial