Hyaline cartilage degenerates after autologous osteochondral transplantation.
OBJECTIVE
Autologous osteochondral grafting is a well-established clinical procedure to treat focal cartilage defects in patients, although basic research on this topic remains sparse. The aim of the current study was to evaluate (1) histological changes of transplanted hyaline cartilage of osteochondral grafts and (2) the tissue that connects the transplanted cartilage with the adjacent cartilage in a sheep model.


METHODS
Both knee joints of four sheep were opened surgically and osteochondral grafts were harvested and simultaneously transplanted to the contralateral femoral condyle. The animals were sacrificed after three months and the received knee joints were evaluated histologically.


RESULTS
Histological evaluation showed a complete ingrowth of the osseous part of the osteochondral grafts. A healing or ingrowth at the level of the cartilage could not be observed. Histological evaluation of the transplanted grafts according to Mankin revealed significantly more and more severe signs of degeneration than the adjacent cartilage, such as cloning of chondrocytes and irregularities of the articular surface.


CONCLUSIONS
We found no connecting tissue between the transplanted and the adjacent cartilage and histological signs of degeneration of the transplanted hyaline cartilage. In the light of these findings, long-term results of autologous osteochondral grafts in human beings have to be followed critically.
Department of Orthopaedics, University Hospital Muenster, Albert-Schweitzer-Str. 33, 48129, Germany. carsten@tibesku.de
C O
CO
Tibesku
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T
Szuwart
T O
TO
Kleffner
P M
PM
Schlegel
U R
UR
Jahn
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H
Van Aken
S
S
Fuchs
D000818:Animals; D002356:Cartilage / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D006818:Hyalin; D007719:Knee Joint / Q000473:pathology / Q000601:surgery; D008297:Male; D010007:Osteochondritis / Q000473:pathology* / Q000601:surgery*; D011183:Postoperative Complications / Q000473:pathology; D012756:Sheep; D014182:Transplantation, Autologous
D024042:Collagen Type I; D024043:Collagen Type II