Cyst-based toxicity tests XVII--prefeeding advantages in short-chronic rotifer microbiotests.
Attempts were made to overcome too-low reproductive output in the 48-h short-chronic microbiotest with the rotifer Brachionus calyciflorus fed with microalgae (Pseudokirchneriella subcapitata) from algal beads older than 4 months (Chemosphere 50 (2002a) 365). Prefeeding of freshly hatched rotifers prior to the start of the actual growth experiments resulted in a satisfactory reproduction (i.e., an intrinsic growth rate r > 0.65), even with subsequent feeding of the rotifers with microalgae from beads stored up to 1 year. Rotirich is an attractive alternative inert food compared with the mixture of microalgae and Spirulina, originally used to prefeed the rotifers. When the rotifers were prefed for 2 h with 30 microg/mL Rotirich, it was then not necessary to add Spirulina to the microalgal food from beads. The above findings are a substantial simplification of the testing procedure of the new short-chronic "culture/maintenance-free" Rotoxkit microbiotest.
Centro de Ciencias del Mary Limnologia, Universidad de Panama, Panama City, Panama.
Chial
C
Belgis
Guido
G
Persoone
D000821:Animal Feed; D000818:Animals; D001426:Bacterial Proteins; D003470:Culture Media; D004032:Diet; D056890:Eukaryota; D033661:Oocysts; D015203:Reproducibility of Results; D012403:Rotifera / Q000254:growth & development* / Q000502:physiology; D053545:Spirulina; D018675:Toxicity Tests / Q000379:methods*
D001426:Bacterial Proteins; D003470:Culture Media