Induction of apoptosis by Saussurea lappa and Pharbitis nil on AGS gastric cancer cells.
We performed this study to understand the molecular basis underlying the antitumor effects of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatments of Saussurea lappa and Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica and Taraxacum mongolicum didn't. FACS analysis and Annexin V staining assay also showed that both Saussurea lappa and Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa, but not Pharbitis nil, increased expression of the p53 and its downstream effector p21Waf1, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa and Pharbitis nil induce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.
Ko
Seong-Gyu
SG
Department of Tumor Biology, Cancer Research Institute, College of Medicine, Seoul National University, Yongon-dong, Chongno-gu, Korea. sgko9209@snu.ac.kr
Koh
Seung-Hee
SH
Jun
Chan-Yong
CY
Nam
Chang-Gyu
CG
Bae
Hyun-Su
HS
Shin
Min-Kyu
MK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004305:Dose-Response Relationship, Drug; D016158:Genes, p53 / Q000187:drug effects; D006801:Humans; D027741:Ipomoea; D010936:Plant Extracts / Q000494:pharmacology; D010943:Plantago; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D030441:Saussurea; D013274:Stomach Neoplasms; D031205:Taraxacum; D013997:Time Factors; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein
D000970:Antineoplastic Agents; C494826:BAX protein, human; C493956:CDKN1A protein, human; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein