Inhibitory activity of 2-nitropropanol against select food-borne pathogens in vitro.
OBJECTIVE
To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis.


RESULTS
Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supplemented with 0-10 mm 2NPOH. Growth rates were inhibited by 2NPOH, with nearly complete inhibition observed with 10 mm. Studies with S. Typhimurium revealed that its survivability during culture in TSB containing 5 or 10 mm 2NPOH was lower (P < 0.05) under aerobic than anaerobic conditions. The survivability of Salmonella during anaerobic culture in TSB containing 2.5 mm 2NPOH was less at pH 5.6 than at pH 7.0 and 8.0. No Salmonella survived anaerobic incubation in TSB supplemented with 10 mm 2NPOH regardless of pH. When incubated in suspensions of freshly collected populations of ruminal and faecal bacteria, Salmonella concentrations were lower (P < 0.05) in suspensions containing 10 mm 2NPOH than in suspensions containing no 2NPOH.


CONCLUSIONS
2NPOH inhibited S. Typhimurium, E. coli O157:H7 and Ent. faecalis.


CONCLUSIONS
Results suggest that 2NPOH may be a useful antimicrobial supplement to reduce carriage of certain food-borne pathogens in food animals.
Jung
Y S
YS
United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, 2881 F and B Road, College Station, TX 77845, USA.
Anderson
R C
RC
Callaway
T R
TR
Edrington
T S
TS
Genovese
K J
KJ
Harvey
R B
RB
Poole
T L
TL
Nisbet
D J
DJ
D000332:Aerobiosis; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D019649:Food Industry / Q000379:methods; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D006863:Hydrogen-Ion Concentration; D009574:Nitro Compounds / Q000494:pharmacology*; D020005:Propanols / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development
C496417:2-nitropropanol; D000900:Anti-Bacterial Agents; D003470:Culture Media; D009574:Nitro Compounds; D020005:Propanols