Structural implication of the induced immune response by Bacillus thuringiensis Cry proteins: role of the N-terminal region.
The potential role of the regions (carboxil and amino) of the Cry proteins in the ability of these proteins to elicit strong immune responses was investigated. Intraperitoneal immunization of mice with the homologous Cry1A protoxins (130-133 kDa), with the long C-terminal half gave rise mostly to similar, strong serum and mucosal IgG and IgM antibody response but a lower induction of these Ab by intranasal route. Remarkably, Cry3A protoxin, devoid of C-terminal half was able to induce a significant mucosal IgG, and IgM Ab as well as Cry1A protoxins, suggesting us that immunogenic abilities are not restricted to C-terminal half but N-terminal half itself could be involved. In fact, this assumption was strengthen by the strong immunogenic abilities of the Cry1A toxins, specially IgG and IgA Ab induced by both routes in different mucosal sites. These data indicate that immunogenic abilities of the Bt Cry proteins reside and depends of the N-terminal half.
FES-IZTACALA, Universidad Nacional Autónoma de México, Biomedicine Av de los Barrios s/n Col. Los Reyes Iztacala 54090 Tlanepantla, Edo de México, Mexico. gloriaguillermina@correo.unam.mx
Gloria Guillermina
GG
Guerrero
Donald H
DH
Dean
Leticia
L
Moreno-Fierros
D000280:Administration, Inhalation; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000276:immunology*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D004731:Endotoxins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D006460:Hemolysin Proteins; D007114:Immunization; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007274:Injections, Intraperitoneal; D051379:Mice
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007070:Immunoglobulin A; D007075:Immunoglobulin M; C030956:insecticidal crystal protein, Bacillus Thuringiensis