KLN-5: a safe monocationic lipophosphoramide to transfect efficiently haematopoietic cell lines and human CD34+ cells.
The safe and efficient delivery of nucleic acids into haematopoietic stem cells (HSCs) has a wide range of therapeutic applications. Although viruses are being used in most clinical trials owing to their high transfection efficacy, recent results highlight many concerns about their use. Synthetic transfection reagents, in contrast, have the advantage of being safe and easy to manage while their low transfection efficiency remains a hurdle that needs to be addressed before they can be widely used. Using information on transfection mechanisms, a new family of monocationic lipids called lipophosphoramides was synthesized. Their efficiency to transfer genes into haematopoietic cell lines (K562, Jurkat and Daudi) and CD34+ cells was assessed. In this study, we report that one of these new compounds, KLN-5, leads to more efficient transfection activity than one of our previously most efficient reagents (EG-308) and the commercially available monocationic lipids (DC-CHOL and DOTAP/DOPE) (P<0.05). In addition, only a slight toxicity related to the chemical structure of the new compounds is observed. Moreover, we show that KLN-5 can successfully carry the transgene into haematopoietic progenitor cells (CD34+). These results demonstrate that synthetic transfection reagents represent a viable alternative to viruses and could have potential practical utility in a number of applications.
Unité INSERM 613, Institut de Synergie des Sciences et de la Santé, Université de Bretagne Occidentale, avenue Foch, 29609 Brest cedex 2, France. Tristan.Montier@univ-brest.fr
T
T
Montier
P
P
Delépine
K
K
Le Ny
Y
Y
Fichou
M
M
Le Bris
E
E
Hardy
E
E
Picquet
J C
JC
Clément
J J
JJ
Yaouanc
C
C
Férec
D000577:Amides; D018952:Antigens, CD34; D001773:Blood Cells / Q000378:metabolism*; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000031:analogs & derivatives*; D005229:Fatty Acids, Monounsaturated; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008055:Lipids; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010714:Phosphatidylethanolamines; D063088:Phosphoramides; D010756:Phosphoric Acids; D010957:Plasmids; D000644:Quaternary Ammonium Compounds; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods* / Q000592:standards; D001616:beta-Galactosidase / Q000235:genetics
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000577:Amides; D018952:Antigens, CD34; D005229:Fatty Acids, Monounsaturated; C493328:KLN-5 compound; D008055:Lipids; D009943:Organophosphorus Compounds; D010714:Phosphatidylethanolamines; D063088:Phosphoramides; D010756:Phosphoric Acids; D000644:Quaternary Ammonium Compounds; C005438:phosphoramide; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; D002784:Cholesterol; D001616:beta-Galactosidase; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane