Single-particle sizing from light scattering by spectral decomposition.
A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum's peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 microm at a wavelength of 0.633 microm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 microm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others.
Semyanov
Konstantin A
KA
Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Institutskaya 3, Novosibirsk 630090 Russia.
Tarasov
Peter A
PA
Zharinov
Alexey E
AE
Chernyshev
Andrei V
AV
Hoekstra
Alfons G
AG
Maltsev
Valeri P
VP
D000465:Algorithms; D048429:Cell Size; D004912:Erythrocytes / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D047569:Laser Scanning Cytometry / Q000379:methods*; D010316:Particle Size; D012542:Scattering, Radiation