Annexin 1 is secreted in situ during ulcerative colitis in humans.
Although annexin l exerts extracellular anti-inflammatory properties, little is known about its release in inflammatory diseases. Here, we characterized annexin 1 secretion in ulcerative colitis (UC) patients. Annexin 1 was detected by immunoblotting, in tissue homogenates and supernatants of colonic biopsies incubated in culture media, and in luminal colonic perfusates of UC patients. Annexin 1 was released by inflamed colonic biopsies from patients having severe UC but not by biopsies from healthy colon of the same patient or by biopsies from non-UC patients or from patients with slight or moderate UC. Annexin 1 was detected in luminal colonic perfusates of patients having moderate or slight UC but not in perfusates from control patients. The level of annexin 1 expression and secretion was unrelated to long-term glucocorticoid treatment, but annexin 1 secretion in perfusates was induced, in some patients, by short-term glucocorticoid exposure. These results show that annexin 1 is secreted endogenously in the colon of patients with UC. This secretion, which occurs both in vitro and in vivo, depends on the severity of inflammation. Given the anti-inflammatory effects of annexin 1, this protein may serve to down-regulate the inflammatory response in the course of inflammatory bowel disease.
Laboratoire de Pharmacologie et Toxicologie, Institut National de la Recherche Agronomique, Toulouse, France. nvergnol@ucalgary.ca
Nathalie
N
Vergnolle
Philippe
P
Pagès
Rosine
R
Guimbaud
Stanislas
S
Chaussade
Lionel
L
Buéno
Jean
J
Escourrou
Christine
C
Coméra
D000328:Adult; D000368:Aged; D017305:Annexin A1 / Q000096:biosynthesis / Q000378:metabolism*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000503:physiopathology*; D003113:Colonoscopy; D015536:Down-Regulation; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged
D017305:Annexin A1