Characterization of the nucleotide sequence of a polyubiquitin gene (PUBC1) from Arabian camel, Camelus dromedarius.
Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.
Al-Khedhairy
Abdulaziz Ali A
AA
Department of Zoology, College of Sciences, P. O. Box 2455, King Saud University, Riyadh 11451, Saudi Arabia. kedhairy@ksu.edu.sa
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D002162:Camelus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D025821:Polyubiquitin / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D025801:Ubiquitin / Q000235:genetics
D025801:Ubiquitin; D025821:Polyubiquitin