Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB.
Clinical and environmental strains of Burkholderia cenocepacia belonging to the recA lineages IIIA and IIIB were examined for exopolysaccharide (EPS) production. The exopolysaccharides structure was determined using mainly gas chromatography coupled to mass spectrometry and NMR spectroscopy. All the strains produced Cepacian, a highly branched polysaccharide constituted of a heptasaccharide repeating unit, composed of one rhamnose, one glucose, one glucuronic acid, one mannose and three galactose residues. This polymer is the most common exopolysaccharide produced by strains of the Burkholderia cepacia (Bcc) complex. One clinical strain produced also another polysaccharide constituted of three galactose units and one 3-deoxy-D-manno-2-octulosonic acid residues, a polymer that was previously isolated from two strains of B. cepacia genomovar I and B. cenocepacia IIIA.
Chiarini
Luigi
L
Unità Biotecnologie, C.R. Casaccia, ENEA, 00060 Rome, Italy.
Cescutti
Paola
P
Drigo
Laura
L
Impallomeni
Giuseppe
G
Herasimenka
Yury
Y
Bevivino
Annamaria
A
Dalmastri
Claudia
C
Tabacchioni
Silvia
S
Manno
Graziana
G
Zanetti
Flavio
F
Rizzo
Roberto
R
D016956:Burkholderia cepacia / Q000145:classification / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D011135:Polysaccharides, Bacterial