PprA: a novel protein from Deinococcus radiodurans that stimulates DNA ligation.
The extraordinary radiation resistance of Deinococcus radiodurans results from the efficient capacity of the bacterium to repair DNA double-strand breaks. By analysing the DNA damage repair-deficient mutant, KH311, a unique radiation-inducible gene (designated pprA) responsible for loss of radiation resistance was identified. Investigations in vitro showed that the gene product of pprA (PprA) preferentially bound to double-stranded DNA carrying strand breaks, inhibited Escherichia coli exonuclease III activity, and stimulated the DNA end-joining reaction catalysed by ATP-dependent and NAD-dependent DNA ligases. These results suggest that D. radiodurans has a radiation-induced non-homologous end-joining repair mechanism in which PprA plays a critical role.
Research Group for Biotechnology Development, Department of Ion-beam-applied Biology, Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan. narumi@taka.jaeri.go.jp
Issay
I
Narumi
Katsuya
K
Satoh
Suzhen
S
Cui
Tomoo
T
Funayama
Shigeru
S
Kitayama
Hiroshi
H
Watanabe
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D011088:DNA Ligases / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011938:Rec A Recombinases / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004247:DNA; D011938:Rec A Recombinases; D011088:DNA Ligases