Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island.
Epithelial cells can respond to conserved bacterial products that are internalized after either bacterial invasion or liposome treatment of cells. We report here that the noninvasive Gram-negative pathogen Helicobacter pylori was recognized by epithelial cells via Nod1, an intracellular pathogen-recognition molecule with specificity for Gram-negative peptidoglycan. Nod1 detection of H. pylori depended on the delivery of peptidoglycan to host cells by a bacterial type IV secretion system, encoded by the H. pylori cag pathogenicity island. Consistent with involvement of Nod1 in host defense, Nod1-deficient mice were more susceptible to infection by cag pathogenicity island-positive H. pylori than were wild-type mice. We propose that sensing of H. pylori by Nod1 represents a model for host recognition of noninvasive pathogens.
Viala
Jérôme
J
Groupe d'Immunité Innée et Signalisation, Institut National de la Santé et de la Recherche Médicale U389, Institut Pasteur, Paris, France.
Chaput
Catherine
C
Boneca
Ivo G
IG
Cardona
Ana
A
Girardin
Stephen E
SE
Moran
Anthony P
AP
Athman
Rafika
R
Mémet
Sylvie
S
Huerre
Michel R
MR
Coyle
Anthony J
AJ
DiStefano
Peter S
PS
Sansonetti
Philippe J
PJ
Labigne
Agnès
A
Bertin
John
J
Philpott
Dana J
DJ
Ferrero
Richard L
RL
D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology / Q000378:metabolism; D000818:Animals; D004847:Epithelial Cells / Q000276:immunology* / Q000378:metabolism; D044404:Genomic Islands; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D053474:Nod1 Signaling Adaptor Protein; D010457:Peptidoglycan / Q000235:genetics / Q000276:immunology*
D048868:Adaptor Proteins, Signal Transducing; D016328:NF-kappa B; C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein; D010457:Peptidoglycan