Identification of the linkage relationship between the flower colour and the content of erucic acid in the resynthesized Brassica napus L.
Brassica napus variety Quantum (yellow flower and low erucic content) as the female parent was crossed with a resynthesized Brassica napus line No. 2127-17 (white flower and high erucic content). The segregation ratios of the flower colour and the erucic acid content were analyzed in the F1, BC1, F2, and DH (doubled haploid) populations. The results indicated that the white flower was dominant over yellow and the erucic acid content was additively inherited. Both traits fit with a monogenic inheritance model, respectively. There were strong evidences to support the linkage relationship between the flower colour and the erucic acid content in the C-genome of B. napus with a recombination frequency of 5.8% in the DH population. The BSA (bulked segregant analysis) strategy was employed to identify random amplified polymorphic DNA (RAPD) marker linked to the genes for the flower colour and erucic acid content in the DH population. Of 685 arbitrary 10-base pair (bp) primers, one primer S92 generated a RAPD marker S92(-1400) that was tightly linked to the genes for the yellow flower and the low erucic acid content in the C-genome of B. napus L. The genetic distance of the genes for yellow flower and the low erucic acid content was 2.2 cM and 5.4 cM from the marker S92(-1400), respectively.
National Key Laboratory of Crop Genetic Improvement, National Rapeseed Genetic Improvement Center, Huazhong Agricultural University, Wuhan 430070, China.
Xue-Ping
XP
Liu
Jin-Xing
JX
Tu
Bao-Yuan
BY
Chen
Ting-Dong
TD
Fu
D029688:Brassica napus / Q000737:chemistry / Q000235:genetics*; D003116:Color; D004883:Erucic Acids / Q000032:analysis*; D035264:Flowers; D008040:Genetic Linkage; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D004883:Erucic Acids; C049811:erucic acid