Reduced renal ClC-5 Cl- channel expression in spontaneously hypertensive rats with microalbuminuria.
Mutations in a renal-specific Cl(-) channel, ClC-5, result in low-molecular-weight proteinuria. Herein we studied ClC-5 expression in the kidneys of spontaneously hypertensive rats (SHR) to identify possible causes of their increased urinary excretion of albumin. The amount of ClC-5 protein was significantly reduced in 3-month-old SHR as compared with normotensive Wistar/Kyoto (WKY) rats. The ClC-5 protein level was partially restored by short term administration of perindopril, an inhibitor of angiotensin-converting enzyme. Corresponding to the increase in ClC-5 expression, the albuminuria in SHR improved to the control level. These results implicate the ClC-5 Cl(-) channel reduction in the development of albuminuria in the early stage of essential hypertension.
Department of Pharmacology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo, 173-8605, Japan. karo@med.teikyo-u.ac.jp
Karo
K
Tanaka
Toshio
T
Nakaki
D000419:Albuminuria / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018118:Chloride Channels / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006973:Hypertension / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020913:Perindopril / Q000627:therapeutic use; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
C097503:CLC-5 chloride channel; D018118:Chloride Channels; D020913:Perindopril