Simultaneous determination of chlorogenic acid, caffeic acid, ferulic acid, protocatechuic acid and protocatechuic aldehyde in Chinese herbal preparation by RP-HPLC.
In the present study, a reversed phase high performance liquid chromatographic (RP-HPLC) method was established for simultaneous determination of chlorogenic acid, caffeic acid, ferulic acid, protocatechuic acid and protocatechuic aldehyde in a Chinese herbal preparation (Fufang-Pugongying-Mixture). The separation was performed on a Hypersil ODS-2 column by isocratic elution with methanol and 0.2 M acetate buffer (pH 3.6) (15 : 85, v/v) as the mobile phase at the flow-rate of 1.0 ml/min with operating temperature of 30 degrees C, and detection wavelength of 300 nm. A good linear regression relationship between peak-areas and concentrations was obtained over the range of 2-200 microg/ml for the five marker compounds mentioned above. The spike recoveries were within 96.72-104.07%. The variation coefficient (CV) values of the precision were in the range of 0.89-4.50%. Moreover the developed method has reference value for quantitative analysis of Taraxacum, Lonicera and Angelica.
Department of Chemistry, Lanzhou University, Lanzhou, China.
Xin-Pu
XP
Li
Jie
J
Yu
Jin-Yin
JY
Luo
Hong-Sheng
HS
Li
Fu-Jie
FJ
Han
Xing-Guo
XG
Chen
Zhi-De
ZD
Hu
D029969:Angelica / Q000737:chemistry; D001547:Benzaldehydes / Q000737:chemistry; D002109:Caffeic Acids / Q000737:chemistry; D002396:Catechols / Q000737:chemistry; D002726:Chlorogenic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry; D015203:Reproducibility of Results; D031205:Taraxacum / Q000737:chemistry; D013696:Temperature
D001547:Benzaldehydes; D002109:Caffeic Acids; D002396:Catechols; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C009091:protocatechuic acid; C005581:protocatechualdehyde; C004999:ferulic acid; C040048:caffeic acid