Biocompatibility test of polyhydroxybutyrate on human cell line.
The human fibroblast MRC-5 cells incubated with PHB granules (TM) added at a final concentration of 4 mg/ml showed a time-course pattern of survival. The percentages of dead cells obtained were at the rate of 3.8% after 7 days, respectively. When the MRC-5 cells grown in different material, using the test concentration of 4 mg/ml PCM, they were found to show a similar time-course increasing pattern of death as that obtained with PHB. However, the death was noted in the cells incubated for 7 days, the death rates obtained was 40.54% respectively.
Raouf
A A
AA
School of Dental Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.
Samsudin
A R
AR
Al-Joudi
F S
FS
Shamsuria
O
O
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006885:Hydroxybutyrates / Q000633:toxicity*; D066298:In Vitro Techniques; D008422:Materials Testing; D011108:Polymers / Q000633:toxicity*; D000091402:Prohibitins
D006885:Hydroxybutyrates; C000717328:PHB protein, human; D011108:Polymers; D000091402:Prohibitins