Human decidual production of hepatocyte-growth factor is not influenced by trophoblastic invasion in vivo.
OBJECTIVE
To evaluate and compare production of hepatocyte growth factor (HGF) from human first-trimester implantation-site decidua (decidua basalis) and nonimplantation site decidua (decidua parietalis), and hence to determine whether human trophoblast invasion in vivo is associated with increased decidual HGF production.


METHODS
Controlled prospective study.


METHODS
University hospital-based study.


METHODS
Ten women undergoing first-trimester termination of singleton pregnancy for psychosocial reasons without preexisting medical or gynecologic diseases.


METHODS
Decidual samples surgically excised and processed for paraffin-embedded immunohistochemistry and for reverse transcription-polymerase chain reaction (RT-PCR) studies.


METHODS
Protein and mRNA production in decidua basalis and decidua parietalis by immunohistochemistry and RT-PCR, respectively.


RESULTS
No statistically significant difference was found between decidua basalis and decidua parietalis in HGF protein or mRNA production. Immunohistochemical analysis (n = 9) showed a mean score of 3.28 +/- 2.37 for decidua basalis and 3.61 +/- 2.66 for decidua parietalis. Semiquantitative analysis of HGF mRNA expression between the two sites showed no statistically significant difference (n = 10)


CONCLUSIONS
Human decidual production of HGF is not influenced by trophoblastic invasion in vivo.
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, Hong Kong, China. meiychoy@cuhk.edu.hk
Mei Yee
MY
Choy
Shing Shun Nelson
SS
Siu
Tse Ngong
TN
Leung
Tze Kin
TK
Lau
D003656:Decidua / Q000000981:diagnostic imaging / Q000378:metabolism*; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D011446:Prospective Studies; D014327:Trophoblasts / Q000502:physiology*; D016216:Ultrasonography, Prenatal
D017228:Hepatocyte Growth Factor