Grb7 expression and cellular migration in chronic lymphocytic leukemia: a comparative study of early and advanced stage disease.
Grb7, a noncatalytic intracellular adaptor protein involved in cell migration, is overexpressed in certain invasive and metastatic solid tumors. We found a highly significant difference in the level of expression of Grb7 between chronic lymphocytic leukemia (CLL) cells obtained from stage I and stage IV patients (P<0.001). Using semiquantitative RT-PCR, we detected high levels of Grb7 in 88% of stage IV patients vs only 18% in stage I patients. A corresponding increase was found in the in vitro migration of stage IV CLL cells in comparison to stage I cells. The statistically significant difference in the expression of Grb7 between stage IV and stage I patients was preserved even when tested specifically in the ZAP70-positive group (P<0.01). These findings show that Grb7 levels reflect the severity of the disease, and may be used, in conjunction with ZAP70, to predict disease progression.
Haran
M
M
Hematology Institute, Kaplan Medical Center, Rehovot, Israel. haran@clalit.org.il
Chebatco
S
S
Flaishon
L
L
Lantner
F
F
Harpaz
N
N
Valinsky
L
L
Berrebi
A
A
Shachar
I
I
D016022:Case-Control Studies; D002465:Cell Movement; D002478:Cells, Cultured; D004194:Disease; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D051382:GRB7 Adaptor Protein; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D009367:Neoplasm Staging; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051746:ZAP-70 Protein-Tyrosine Kinase
C495955:GRB7 protein, human; D011506:Proteins; D051382:GRB7 Adaptor Protein; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human