Fluorescent in situ hybridization analysis of Philadelphia chromosome-negative chronic myeloid leukemia with the bcr/abl fusion gene.
This report describes a patient with Philadelphia chromosome-negative (Ph-) but bcr/abl fusion gene-positive chronic myeloid leukemia (CML) and a molecular analysis of the mechanisms behind the Ph status. Spectral karyotyping-fluorescent in situ hybridization (SKY-FISH) analysis showed no abnormal translocation; however, a bcr/abl fusion gene was detected by reverse transcriptase-polymerase chain reaction analysis. FISH analysis showed that signals from the 9q and 22q subtelomere probes were detected on the der(9) and der(22) chromosomes, respectively. On the other hand, FISH analysis of the abl and bcr genes with dual fusion probes, which can detect the bcr/abl fusion gene on both the der(9) and der(22) chromosomes, showed the signal for bcr/abl fusion on the der(22) chromosome but not on the der(9) chromosome. These results indicate that insertion of the abl gene into the bcr region on the der(22) chromosome or retranslocation between the der(9) chromosome and the der(22) chromosome may have caused the Ph CML in this case.
Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie, Japan.
Fumihiko
F
Monma
Kazuhiro
K
Nishii
Shunji
S
Yamamori
Noboru
N
Hosokai
Takahiro
T
Nakazaki
Felipe
F
Lorenzo
Eiji
E
Usui
Miho
M
Sakakura
Hiroyuki
H
Miyashita
Atsushi
A
Fujieda
Kohshi
K
Ohishi
Naoyuki
N
Katayama
Hiroshi
H
Shiku
D000328:Adult; D005260:Female; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D054438:Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative / Q000235:genetics*; D008677:Metaphase; D020133:Reverse Transcriptase Polymerase Chain Reaction