Differential accumulation of Glut1 in the non-DRM domain of the plasma membrane in response to the inhibition of oxidative phosphorylation.
Approximately 50% of Glut1 in the plasma membrane of Clone 9 cells is localized to the detergent-resistant membrane (DRM) fraction. Acute exposure (90 min) to 5mM azide stimulated glucose transport by approximately 4.7-fold and increased the abundance of Glut1 in the non-DRM fraction of the plasma membrane by approximately 2.9-fold while the abundance of Glut1 in the DRMs was not changed. In parallel experiments, approximately 17 h exposure to azide further increased the rate of glucose transport over that observed at 90 min by approximately 33% and increased plasma membrane Glut1 content by approximately 3.5-fold over control. The increase in total plasma membrane Glut1 reflected a approximately 4.7-fold increase of Glut1 content in the non-DRM fraction and a approximately 2.6-fold increase in the DRMs. We conclude that acute exposure to azide increases Glut1 content in the non-DRM fractions, while prolonged exposure to azide increases the Glut1 content in both non-DRM and DRM fractions. These changes may play an important role in the stimulation of glucose transport in response to the inhibition of oxidative phosphorylation.
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
Darrell
D
Rubin
Faramarz
F
Ismail-Beigi
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002999:Clone Cells; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D007150:Immunohistochemistry; D007700:Kinetics; D008099:Liver / Q000166:cytology; D021962:Membrane Microdomains / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D013347:Subcellular Fractions / Q000737:chemistry / Q000648:ultrastructure
D001386:Azides; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495679:Slc2a1 protein, rat; D005947:Glucose