[Gene expression analysis of near-isogenic lines for different heterosis in maize].
In this report, we used three pairs of near-isogenic lines( NILs) 478,488-1 and 488-2, which are different in seed size and leaves above the top ear, to make a survey of the expressions of six genes in leaf tissues of these NILs series in order to find whether these genes serve as models for the rate-limiting genes in the heterosis. Our results showed that some of genes may play a role in heterosis response either through increased or decreased expression. When all six genes were considered as a whole, they showed a tendency for higher gene expression in 488-1 than in 488-2 and in 478, and in 488-1 x 340 than in 478 x 340 and in 488-2 x 340. Sus1, Sh1 and ZmSps34-3' were good candidate genes because they have been proposed to be rate-limiting for the seed size and grain weight of the individuals heterosis. And also, in order to seek a molecular genetic dissection of heterosis, two main theories, dominance and overdominance, which hold the classical explanation for the heterosis, were discussed too.
College of Life Science, Shandong Agricultural University, Taian 271018, China. zeliwang@yahoo.com
Ze-Lio
ZL
Wang
Lu-Xin
LX
Wang
Yong-Hui
YH
Zhang
Jun-Ye
JY
Ma
D015152:Blotting, Northern; D015870:Gene Expression; D006823:Hybrid Vigor; D012333:RNA, Messenger / Q000032:analysis; D003313:Zea mays / Q000235:genetics*
D012333:RNA, Messenger