[Conjugates of the Ca2+ -regulated photoprotein obelin with immunoglobulins: synthesis and use as labels in bioluminescent immunoassay].
An efficient procedure for obelin conjugation with immunoglobulins was developed. The possibility was shown of using the resulting conjugates instead of a radioisotope label for the immunoassay of thyroid stimulating hormone in sera; the conjugates provide a sensitivity of 0.01 microIU/ml. The results of bioluminescent immunoassay (sera of 34 patients) satisfactorily correlate with the results of radioisotope assay (R 0.99).
Frank
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LA
Petunin
A I
AI
Vysotskiĭ
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D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D008164:Luminescent Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000276:immunology; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000097:blood
D007074:Immunoglobulin G; D008164:Luminescent Proteins; D011994:Recombinant Proteins; C010497:obelin; D013972:Thyrotropin; D002118:Calcium