Genotoxicity of aspartame.
In the present study, the genotoxic effects of the low-calorie sweetener aspartame (ASP), which is a dipeptide derivative, was investigated using chromosome aberration (CA) test, sister chromatid exchange (SCE) test, micronucleus test in human lymphocytes and also Ames/Salmonella/ microsome test. ASP induced CAs at all concentrations (500, 1000 and 2000 microg/ml) and treatment periods (24 and 48 h) dose-dependently, while it did not induce SCEs. On the other hand, ASP decreased the replication index (RI) only at the highest concentration for 48 h treatment period. However, ASP decreased the mitotic index (MI) at all concentrations and treatment periods dose-dependently. In addition, ASP induced micronuclei at the highest concentrations only. This induction was also dose-dependent for 48 hours treatment period. ASP was not mutagenic for Salmonella typhimurium TA98 and TA100 strains in the absence and presence of S9 mix.
Biology Department, Faculty of Arts and Sciences, Natural and Applied Sciences Institute, Cukurova University, Adana, Turkey. reyyup@mail.cu.edu.tr
Eyyüp
E
Rencüzoğullari
Berrin Ayaz
BA
Tüylü
Mehmet
M
Topaktaş
Hasan Basri
HB
Ila
Ahmet
A
Kayraldiz
Mehmet
M
Arslan
Songül Budak
SB
Diler
D000818:Animals; D001218:Aspartame / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013549:Sweetening Agents / Q000633:toxicity*
D009153:Mutagens; D013549:Sweetening Agents; D001218:Aspartame