[Single nonselective cation channels activated by muscarinic agonists in smooth muscle cells of the guinea-pig small intestine].
The carbachol-evoked inward cationic current in guinea-pig ileum smooth muscle cells is comprised of three types nonselective cationic channels (NSCC) with small (10 +/- 2 pS), medium (56 +/- 8pS) and large (135 +/- 14 pS) unitary conductance. All three types of NSCC could be activated by external application of carbachol as well as by internal application of GTPgS. It was found that behavior of carbachol- and GTPgammaS-evoked whole-cell current is mainly determined by the properties of medium conductance channels. The U-shaped I-V relationship of the whole-cell cationic current at negative potentials range arrives from voltage-dependence of its Po of this channel.
A V
AV
Dresviannikov
A V
AV
Zholos
M F
MF
Shuba
D000818:Animals; D002412:Cations / Q000378:metabolism; D002478:Cells, Cultured; D006168:Guinea Pigs; D007082:Ileum / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects*; D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D018721:Muscarinic Agonists / Q000494:pharmacology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011976:Receptors, Muscarinic / Q000378:metabolism*
D002412:Cations; D007473:Ion Channels; D018721:Muscarinic Agonists; D011976:Receptors, Muscarinic