Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation.
OBJECTIVE
To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG).


RESULTS
Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres.


CONCLUSIONS
Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. SIGNIFICANCE OF AND IMPACT OF THE STUDY: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.
Centro de Engenharia Biológica, Universidade do Minho, Minho, Portugal.
N
N
Cerca
G B
GB
Pier
M
M
Vilanova
R
R
Oliveira
J
J
Azeredo
D000117:Acetylglucosamine / Q000096:biosynthesis*; D018441:Biofilms / Q000254:growth & development*; D018533:Biomass; D006384:Hemagglutination; D006801:Humans; D013212:Staphylococcus epidermidis / Q000254:growth & development* / Q000378:metabolism
C113831:poly-N-acetyl glucosamine; D000117:Acetylglucosamine