Initial blood washout during organ procurement determines liver injury and function after preservation and reperfusion.
Organ procurement is the first step toward effective liver preservation and comprises a thorough washout of blood components from the microvasculature. To study the efficacy of optimal blood washout of the liver, three groups were compared including low-pressure perfusion with UW-CSS (12 mmHg, group A), which is the routine method in clinical practice, high-pressure perfusion with UW-CSS (100 mmHg, group B) and low-pressure perfusion with modified UW solution (12 mmHg, group C). After procurement all livers were preserved in original UW-CSS for 0, 24 or 48 h, followed by reperfusion in oxygenated Williams Medium E for 24 h at 37 degrees C. Histology results of livers procured in group A, showed good hepatocyte viability but also remaining erythrocytes. However, injury parameters were high and ATP concentrations were low. No functional differences were found. Group B, high pressure, and group C, modified UW-CSS, both showed better results. High-pressure washout is preferable since the warm ischemia time during procurement is short. We propose to use high-pressure UW-CSS perfusion for the initial blood washout of the donor liver instead of the usually used low-pressure washout.
't Hart
Nils A
NA
University Hospital Groningen, Surgery Research Laboratory, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. n.a.hart@path.azg.nl
van der Plaats
Arjan
A
Leuvenink
Henri G D
HG
Wiersema-Buist
Janneke
J
Olinga
Peter
P
van Luyn
Marja J A
MJ
Verkerke
Gijsbertus J
GJ
Rakhorst
Gerhard
G
Ploeg
Rutger J
RJ
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000032:analysis; D000493:Allopurinol; D000818:Animals; D001790:Blood Physiological Phenomena; D004906:Erythrocyte Count; D005978:Glutathione; D006498:Hepatectomy / Q000379:methods*; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology; D007328:Insulin; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000502:physiology; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D008930:Mitochondria, Liver / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000379:methods; D020858:Tissue and Organ Harvesting / Q000379:methods*
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose