Endothelial cell culture: beginnings of modern vascular biology.
Endothelial cells derived from human umbilical veins were first successfully cultured in vitro in 1973. Weibel-Palade bodies and the von Willebrand factor antigen were used as morphological, immunohistochemical, and functional markers to unequivocally identify the cells. These landmark studies helped initiate the growth of modern vascular biology.
Department of Medicine, Weill Medical College of Cornell University, New York, New York, USA.
Ralph L
RL
Nachman
Eric A
EA
Jaffe
D018929:Cell Culture Techniques / Q000266:history* / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D010974:Platelet Aggregation; D014471:Umbilical Veins / Q000166:cytology