The combined regulation of estrogen and cyclic tension on fibroblast biosynthesis derived from anterior cruciate ligament.
Female athletes are two to eight times more likely to suffer a knee or ankle ligament injury than male athletes, and sex hormones have been considered to play an important role in the injury. Because ligaments are always under mechanical loading during sports, mechanical force is also a critical factor in ligament injuries. In this study, the effects of estrogen and mechanical loading on the gene expression of three major components of ligament--collagen type I, type III, and biglycan--in primary cultured porcine anterior cruciate ligament (ACL) fibroblasts were investigated individually and collectively using reverse transcript-polymerase chain reaction (RT-PCR). The results revealed that cyclic tensile loading alone increased the messenger RNA expression of collagen I but did not affect that of collagen III and biglycan, and estrogen alone increased the gene expression of collagen I and III but not of biglycan. However, combined administration of estrogen and cyclic loading inhibited the mRNA expression of all the three genes. These results suggested that the inhibition of the gene expression of major extracellular matrix component molecules caused by the combined effects of estrogen and mechanical loading, unique to females, might be responsible for the increased incidence of ligaments injury in female athletes.
Lee
Chun-Yi
CY
Department of Orthopaedic Surgery, China Medical University Hospital, Taichung, Taiwan.
Liu
Xuhui
X
Smith
Carolyn L
CL
Zhang
Xiaoliu
X
Hsu
Horng-Chaung
HC
Wang
Der-Yean
DY
Luo
Zong-Ping
ZP
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000378:metabolism*; D058578:Biglycan; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D024061:Collagen Type III / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D011509:Proteoglycans / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D013552:Swine
D058578:Biglycan; D024042:Collagen Type I; D024061:Collagen Type III; D004967:Estrogens; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D004958:Estradiol