Combined use of a collagen-based dermal substitute and a fibrin-based cultured epithelium: a step toward a total skin replacement for acute wounds.
Integra, a dermal replacement, is used as an immediate and temporary coverage for acute wounds, after which, autograft is used to reconstitute permanently the epidermal coverage. The fibrin sheet-cultured epithelium autograft (FS-CEA) could provide an effective alternative to the surgical procedure. To evaluate this hypothesis, we compared the association of Integra/FS-CE to Integra/control-cultured epithelium (control-CE). Their respective abilities: (1) to produce dermal-epidermal construct in vitro; (2) to generate skin replacement when grafted onto athymic mice were studied. We have shown that: (1) 83% of the FS-CE attached to the artificial dermis in vitro compared to only 33% for control-CE; (2) retraction of the grafted area was significantly lower 2 weeks after grafted with FS-CE than with the control-CE (P < 0.05); (3) 83% of the mice grafted with FS-CE showed the presence of a differentiated human epidermis 21 days after grafting, while such an epidermis was absent in all the animals of the control-CE group. We found that the use of FS-CE greatly improved adhesion, development of the epithelium and graft take onto the artificial dermis. We believe this technology should significantly improve the performance of dermal-epidermal skin replacement for acute wounds.
Mis
Béatrice
B
Isotis SA, 18-20 Avenue de Sévelin, 1004 Lausanne, Switzerland. beatrice.mis@isotis.com
Rolland
Eric
E
Ronfard
Vincent
V
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002478:Cells, Cultured; D002809:Chondroitin Sulfates; D003094:Collagen; D004817:Epidermis / Q000473:pathology; D004848:Epithelium / Q000637:transplantation; D005337:Fibrin; D006085:Graft Survival; D015603:Keratinocytes / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D017768:Skin, Artificial
D001672:Biocompatible Materials; C114835:integra artificial skin; D005337:Fibrin; D002809:Chondroitin Sulfates; D003094:Collagen