Systematic gene targeting on the X chromosome of Drosophila melanogaster.
The genome of the model organism Drosophila melanogaster has been sequenced and annotated. Based on this groundwork, we performed a systematic genetic screen of the D. melanogaster X chromosome, which carries about one sixth of the genes of the organism. We generated a collection of single P-element insertions to provide genetic and molecular access to virtually all X-chromosomal genes. The study complements earlier work designed to systematically identify vital genes on the X chromosome by targeting transcription units which are phenotypically silent. We describe single UAS sequence-bearing P-element insertions throughout the X chromosome, which allows one to express the tagged genes under control of tissue/organ-directed GAL4 activity. In addition, the present collection of single insertion lines provides a tool to generate chromosomal deletions which are on average less than 33 kb in size.
Beinert
Nicole
N
Max-Planck-Institut für biophysikalische Chemie, Abt. Molekulare Entwicklungsbiologie, Am Fassberg, 37077 Göttingen, Germany.
Werner
Meike
M
Dowe
Gordon
G
Chung
Ho-Ryun
HR
Jäckle
Herbert
H
Schäfer
Ulrich
U
D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D018390:Gene Targeting; D017344:Genes, Insect / Q000235:genetics* / Q000502:physiology; D005804:Genes, Lethal; D016254:Mutagenesis, Insertional / Q000235:genetics; D014960:X Chromosome / Q000235:genetics*
D004251:DNA Transposable Elements