Estimation of trace amounts of chromium(III) in multi-vitamin with multi-mineral formulations.
Two new specific, selective, simple and inexpensive spectroscopic methods for estimating a trace amount of chromium (Cr3+) from a multi-vitamin with multi-mineral pharmaceutical formulations were developed. The proposed methods are based on the conversion of Cr3+ to Cr6+ either by oxidation with a nitric acid-perchloric acid mixture (method I) or by fusion with an excess amount of sodium carbonate (method II), followed by the complexation of Cr6+ with 1,5-diphenylcarbazide (DPC) in a mineral acidic solution of pH 1.0 +/- 0.5. The pink-colored complex was estimated at 544 nm. Both methods were found to be linear in the range of 0.1 - 0.8 microg/ml with a limit of detection in the range of 0.0123 - 0.0157 microg/ml and a limit of quantitation in the range of 0.0419 - 0.0525 microg/ml. Method I was found to be suitable for estimating Cr3+ species in various formulations, like tablets, capsules and syrups, while method II was found to be suitable for tablets and capsules. Satisfactory recovery from spiked samples of standard Cr3+ suggests no interference of any excipients and diverse ions present in the formulations. The developed methods were compared with AAS by ANOVA, and no significant difference was observed.
Pharmacy Department, Faculty of Technology & Engineering, The Maharaja Sayajirao University of Baroda, Kalabhavan, Vadodara, Gujarat 390001, India.
Jolly M
JM
Sankalia
Rajashree C
RC
Mashru
Mayur G
MG
Sankalia
Priti P
PP
Parikh
Vijay B
VB
Sutariya
D002138:Calibration; D002857:Chromium / Q000032:analysis*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008903:Minerals; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014815:Vitamins
D008903:Minerals; D004364:Pharmaceutical Preparations; D014815:Vitamins; D002857:Chromium