[Inhibition of grape procyanidin on the expression of SSTR-2 mRNA in the hepatic cells induced by N-nitroso compounds].
OBJECTIVE
To study the effect of grape procyanidin (GPC) on the expression of somatostatin receptor 2 mRNA induced by N-nitroso compounds in the hepatic cells of rats.


METHODS
NaNO2 was fed to Wistar rats to induce hepatocytes' mutation, and GPC was given in dose of 100mg/kg and 10mg/kg respectively through mouths to the rats of the two experimental groups at the same time. After eight weeks, situ hybridization was applied to measure the expression level of SSTR-2 mRNA in rats' hepatic cells.


RESULTS
The positive cells rate of SSTR-2 mRNA expressed in the injury comparison group and the high-dose GPC group were 19.89% and 7.83% respectively and the difference between the two groups had statistical significance (P < 0.05).


CONCLUSIONS
GPC had inhibitory effect on N-nitroso compounds-induced abnormal expression of SSTR-2 mRNA in hepatic cells of rats.
Institute of Nutrition, Medical College of Qingdao University, Qingdao 266021, China.
Jinyi
J
Zhong
Hongqun
H
Cong
D000818:Animals; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009603:Nitroso Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity; D044945:Proanthocyanidins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry*
D044946:Biflavonoids; D009603:Nitroso Compounds; D044945:Proanthocyanidins; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; C017674:procyanidin; D002392:Catechin; C093733:somatostatin receptor 2