Stylopine from Chelidonium majus inhibits LPS-induced inflammatory mediators in RAW 264.7 cells.
Stylopine is a major component of the leaf of Chelidonium majus L. (Papaveraceae), which has been used for the removal of warts, papillomas and condylomas, as well as the treatment of liver disease, in oriental countries. Stylopine per se had no cytotoxic effect in unstimulated RAW 264.7 cells, but concentration-dependently reduced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta), and the IL-6 production and cyclooxygenase-2 (COX-2) activity caused by the LPS stimulation. The levels of inducible nitric oxide synthase (iNOS) and COX-2 protein expressions were markedly suppressed by stylopine in a concentration dependent manner. These results suggest that stylopine suppress the NO and PGE2 production in macrophages by inhibiting the iNOS and COX-2 expressions. These biological activities of stylopine may contribute to the anti-inflammatory activity of Chelidonium majus.
Jang
Seon Il
SI
Department of Skin & beauty, Seojeong College, Yangju 482-860, Korea.
Kim
Byung Hee
BH
Lee
Woo-Yiel
WY
An
Sang Jin
SJ
Choi
Han Gil
HG
Jeon
Byung Hun
BH
Chung
Hun-Taeg
HT
Rho
Jung-Rae
JR
Kim
Young-Jun
YJ
Chai
Kyu-Yun
KY
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D028281:Chelidonium; D004305:Dose-Response Relationship, Drug; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D018515:Plant Leaves; D018517:Plant Roots
D000893:Anti-Inflammatory Agents; D001600:Berberine Alkaloids; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C014212:stylopine