Biochemical methods to assess CFTR expression and membrane localization.
Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.
Farinha
Carlos M
CM
Centre of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal. cfarinha@igc.gulbenkian.pt
Penque
Deborah
D
Roxo-Rosa
Mónica
M
Lukacs
Gergely
G
Dormer
Robert
R
McPherson
Margaret
M
Pereira
Malcolm
M
Bot
Alice G M
AG
Jorna
Huub
H
Willemsen
Rob
R
Dejonge
Hugo
H
Heda
Ghanshyam D
GD
Marino
Christopher R
CR
Fanen
Pascale
P
Hinzpeter
Alexandre
A
Lipecka
Joanna
J
Fritsch
Janine
J
Gentzsch
Martina
M
Edelman
Aleksander
A
Amaral
Margarida D
MD
D019921:Biotinylation; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000302:isolation & purification*; D005821:Genetic Techniques; D006031:Glycosylation; D006801:Humans; D007158:Immunologic Techniques
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator