Alternation of cytosolic carbonic anhydrase isoenzymes during deciduomatal development in pregnant mice.
OBJECTIVE
To analyze the activity of cytosolic carbonic anhydrases (CAs) during the pre- and postimplantation stages of pregnancy in mice. Furthermore, to investigate the CA activity in mice in which abortion was induced by injection of substance P.


METHODS
Controlled animal experiment.


METHODS
University research laboratory.


METHODS
A total of 75 ICR mice (weight between 20-25 g) that showed two consecutive 4-day cycles.


METHODS
Substance P (2.4 microg/g) or CA inhibitor (100 microg/g), or both, were administered. Decidualized uterine tissues were collected on days 0.5-10.5 after administration.


METHODS
The activity, protein expression pattern, and mRNA level of decidual cytosolic CAs.


RESULTS
The abortion induced by injection of substance P led to an aberrant expression of cytosolic CA and a decreased number of embryos. Furthermore, substance P-induced abortion could be effectively inhibited by CA inhibitors.


CONCLUSIONS
Cytosolic CAs, especially CA II, may act as negative regulators in implantation, development, and maintenance of the pregnancy and therefore, this information could be further applied in developing therapies for human sterility.
Chiang
Whei-Ling
WL
School of Medical Technology, Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan.
Liu
Jer-Yuh
JY
Liao
Chu-Yu
CY
Yang
Shun-Fa
SF
Hsieh
Yih-Shou
YS
Chu
Shu-Chen
SC
D000019:Abortifacient Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000028:Abortion, Induced; D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology*; D003656:Decidua / Q000166:cytology / Q000254:growth & development*; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D013373:Substance P / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000019:Abortifacient Agents; D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D012333:RNA, Messenger; D013373:Substance P; D002256:Carbonic Anhydrases