Antigens up the nose: identification of putative biomarkers for nasal tolerance induction functional studies combined with proteomics.
Intranasal autoantigen delivery is the most effective means of inducing mucosal tolerance and suppression of autoimmune disease. In an effort to identify markers of the "tolerant state", we employed proteomics technology at the level of the cervical lymph node. The analysis revealed that nasal antigen administration (without adiuvant) led to modulation of various proteins among which the most prominent were haptoglobin, nonintegrin 67 kDa laminin receptor, and MRP8. The immunoregulatory haptoglobin may qualify as (bio)marker for effective immunotherapy.
Department of Target Discovery and Pharmacology at NV Organon, Oss, The Netherlands.
Annemieke M
AM
Boots
Peter D
PD
Verhaert
Rezie J
RJ
te Poele
Sabine
S
Evers
Christina J
CJ
Coenen-de Roo
Jenny
J
Cleven
Ebo S
ES
Bos
D054392:Adipokines; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D040501:Calgranulin A / Q000032:analysis / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D000071451:Chitinase-3-Like Protein 1; D016207:Cytokines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000276:immunology; D006242:Haptoglobins / Q000032:analysis / Q000378:metabolism; D007108:Immune Tolerance / Q000276:immunology*; D007114:Immunization; D037102:Lectins; D008198:Lymph Nodes / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D021381:Protein Transport / Q000276:immunology; D040901:Proteomics; D016601:RNA-Binding Proteins; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D017474:Receptors, Laminin / Q000032:analysis / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
D054392:Adipokines; D015415:Biomarkers; C084533:CHI3L1 protein, human; D040501:Calgranulin A; D002352:Carrier Proteins; D000071451:Chitinase-3-Like Protein 1; D016207:Cytokines; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D006242:Haptoglobins; D037102:Lectins; C111390:OVA 323-339; C121375:Pcbp1 protein, mouse; D010446:Peptide Fragments; D016601:RNA-Binding Proteins; C499308:Rag2 protein, mouse; D011948:Receptors, Antigen, T-Cell; D017474:Receptors, Laminin; C064655:V(D)J recombination activating protein 2; D010047:Ovalbumin