[Study on the distributive regulation of Legionella pneumophila in different water].
OBJECTIVE
To grasp the distributive regulation and typing characters of Legionella pneumophila (Lp) in water.


METHODS
Take water samples from the natural and living environment, Suspect strains were cultivated routinely from condensed water samples, then dual identification of serology identification and 16S rRNA semi-nested PCR technology were used to identify typings of Legionella and Lp.


RESULTS
28 water samples were detected Legionella positive of the whole 126 sampling sites (21 Lp positive among those). The legionella positive rates of sampling sites chosen in the natural and living environment were 32.1% and 22.2% respectively (Lp positive rates were 21.4% and 16.7% respectively); 36 samples were legionella positive of the 430 samples through a year (23 samples were Lp positive). The detection of positive rates were higher in summer and autumn, and also higher in air-conditioner cooling tower water, shower water and lake water in park.


CONCLUSIONS
Lps are widely distributed over all kinds of water of the natural and living environment; the ambient temperature is an important factor for Lp's contamination, summer and autumn are the seasons with high incidence when Legionella contaminate and Legionellosis emit, and they also could be found in spring and winter. People contact closely with these water, such as park water, air-conditioner cooling tower water, shower water etc., which are high risks of legionella contamination and legionellosis transmission. Necessary measures should be taken promptly to protect public health.
Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China.
Hongliang
H
Liu
Changchun
C
Hou
Ying
Y
Xu
Aihua
A
Huang
Xuemin
X
Chen
D015373:Bacterial Typing Techniques; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014871:Water Microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S