Pyrosequencing: sequence typing at the speed of light.
Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.
Scottish Meningococcus and Pneumococcus Reference Laboratory, Department of Microbiology, House on the Hill, Stobhill Hospital, Balornock Road, Glasgow, G21 3UW.
Matthew A
MA
Diggle
Stuart C
SC
Clarke
D015373:Bacterial Typing Techniques / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D005798:Genes, Bacterial / Q000235:genetics*; D008954:Models, Biological; D009345:Neisseria meningitidis / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013997:Time Factors
D017931:DNA Primers; D004247:DNA