Antioxidant activity of NADH and its analogue--an in vitro study.
The antioxidant activities of NADH and of its analogue, 1,4-dihydro-2,6-dimethyl-3,5-dicarbethoxy-pyridine (PyH(2)), were evaluated in vitro. NADH was found to be oxidized by the peroxyl radical derived from 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) decomposition, in a pH-dependent manner. Both NADH and PyH(2) inhibited the peroxidation of egg yolk lecithin (EYL) liposomes, although PyH(2) was more effective than NADH when 2,2'-azobis-4-methoxy-2,4-dimethyl-valeronitrile (methoxy-AMVN) was employed to induce EYL liposome peroxidation. The antioxidant activities of NADH and PyH(2) were also evaluated by measuring their influences on 1,3-diphenylisobenzofuran (DPBF) fluorescence decay in the presence of peroxyl radicals. NADH and PyH(2) were much more effective at inhibiting DPBF quenching in Triton X-100 micelles than in liposomes. These results indicate that NADH can inhibit lipid peroxidation despite being hydrophilic. Nevertheless, membrane penetration is an important factor and limits its antioxidant activity.
Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport, Wiejska 1, 80-336 Gdansk, Poland.
Robert Antoni
RA
Olek
Wieslaw
W
Ziolkowski
Jan Jacek
JJ
Kaczor
Lucedio
L
Greci
Jerzy
J
Popinigis
Jedrzej
J
Antosiewicz
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004530:Egg Yolk / Q000737:chemistry; D005609:Free Radicals / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry / Q000378:metabolism*
D000975:Antioxidants; D005609:Free Radicals; D008081:Liposomes; D010713:Phosphatidylcholines; D011725:Pyridines; D009243:NAD