Cytoprotective effect on oxidative stress and inhibitory effect on cellular aging of Terminalia chebula fruit.
The ethanol extract from the fruit of Terminalia chebula (Combretaceae) exhibited significant inhibitory activity on oxidative stress and the age-dependent shortening of the telomeric DNA length. In the peroxidation model using t-BuOOH, the T. chebula extract showed a notable cytoprotective effect on the HEK-N/F cells with 60.5 +/- 3.8% at a concentration of 50 microg/ml. In addition, the T. chebula extract exhibited a significant cytoprotective effect against UVB-induced oxidative damage. The life-span of the HEK-N/F cells was elongated by 40% as a result of the continuous administration of 3 microg/ml of the T. chebula extract compared to that of the control. These observations were attributed to the inhibitory effect of the T. chebula extract on the age-dependent shortening of the telomere, length as shown by the Southern blots of the terminal restriction fragments (TRFs) of DNA extracted from subculture passages.
College of Pharmacy, Chungnam National University, Daejeon, Korea.
MinKyun
M
Na
KiHwan
K
Bae
Sam Sik
SS
Kang
Byung Sun
BS
Min
Jae Kuk
JK
Yoo
Yuko
Y
Kamiryo
Yu-ichiro
Y
Senoo
Seiichi
S
Yokoo
Nobuhiko
N
Miwa
D004247:DNA / Q000187:drug effects; D005638:Fruit; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D016615:Telomere / Q000187:drug effects; D029766:Terminalia; D014466:Ultraviolet Rays
D010936:Plant Extracts; D020011:Protective Agents; D004247:DNA