Imaging of gene expression during long-term potentiation.
Long term potentiation (LTP) at hippocampal Schaffer collateral-CA1 synapses involves an early and a late phase, where only the latter is sensitive to protein synthesis inhibitors. Here we characterized the dynamics of protein synthesis associated with the induction of L-LTP using a transgenic mouse model in which a cAMP responsive element (CRE)-regulated promoter drives production of an enhanced yellow fluorescent protein (eYFP). We found that eYFP fluorescence increased after less than 30 min following L-LTP induction. Application of transcription and translation suppressors and the NMDA receptor antagonist D-AP5 inhibited the L-LTP and prevented the rise in eYFP levels. The early-phase of LTP was not affected by inhibiting protein synthesis.
Laboratory for Neuronal Circuit Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.
Thomas
T
Behnisch
Shinichi
S
Matsushita
Thomas
T
Knöpfel
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004558:Electric Stimulation / Q000379:methods; D004594:Electrophysiology / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism*; D051670:Shaw Potassium Channels; D013997:Time Factors
D001426:Bacterial Proteins; D018691:Excitatory Amino Acid Antagonists; C490839:Kcnc1 protein, mouse; D008164:Luminescent Proteins; D024642:Potassium Channels, Voltage-Gated; D011500:Protein Synthesis Inhibitors; D051670:Shaw Potassium Channels; C054562:yellow fluorescent protein, Bacteria; D015763:2-Amino-5-phosphonovalerate