[Saline tolerance white clover transformed with the betaine aldehyde dehyrogenase gene by Agrobacterium tumefaciens].
The white clover has been transformed with the Betaine Aldehyde Dehydrogenase (BADH) gene cloned from Atriplex hortensis by Agrobacterium tumefaciens. The relative electronic conductivity of the transgenic plants under 1% NaCl stress for 48 hours was about 20%, less than the control plant's relative electronic conductivity (more than 40%), these showed the cell membrane of the transgenic plants has been less injured than control plants under salt stress. The other experience showed that the transgenic plant could grow well in water culture included 0.5% NaCl for more than two weeks, but the control plants could not.
Chen
Chuan-Fang
CF
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. chuanfangchen@hotmail.com
Li
Yi-Wen
YW
Chen
Yu
Y
Bai
Jian-Rong
JR
Li
Hui
H
Zhu
Yin-Feng
YF
Chen
Shou-Yi
SY
Jia
Xu
X
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D050728:Betaine-Aldehyde Dehydrogenase; D015152:Blotting, Northern; D015139:Blotting, Southern; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology*; D014170:Transformation, Genetic; D029921:Trifolium / Q000187:drug effects / Q000235:genetics*
D012965:Sodium Chloride; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase