Association of calcium phosphate and fibroblast growth factor-2: a dynamic light scattering study.
The size distributions of fibroblast growth factor-2 (FGF-2) in aqueous solutions with neutral pH were investigated with a dynamic light scattering technique. We found that the FGF-2 was distributed in dimer or trimer form at concentrations of 0.1-1.0 mg . mL(-1). An aggregate with a hydrodynamic radius of approximately 90 nm coexisted with this and its proportion increased with a decrease in concentration. At lower concentrations (less than 0.10 mg . mL(-1)) FGF-2 aggregates with an average radius of 80-100 nm were dominant and were stable for more than a day. These FGF-2 solutions were mixed with calcium phosphate solutions to produce a sub-micron sized compound of FGF-2 and hydroxyapatite, which could be used as a biological implant that possessed a pharmacological function for bone formation. By utilizing a transformation from amorphous calcium phosphate to hydroxyapatite, FGF-2 was effectively incorporated into polycrystals of hydroxyapatite.SEM photograph of a mixture of hydroxyapatite and FGF-2.
Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Kazuo
K
Onuma
Noriko
N
Kanzaki
Naoko
N
Kobayashi
D002130:Calcium Phosphates; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006801:Humans; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D012542:Scattering, Radiation
D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; C519480:amorphous calcium phosphate; D016222:Fibroblast Growth Factor 2; D017886:Durapatite