Non-PCR methods for the analysis of CFTR transcripts.
Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very low levels of its endogenous expression. In this paper, we describe four different non-PCR methods optimized to analyze CFTR transcripts in epithelial cell lines, primary cell lines and native tissues that express significant amounts of CFTR transcript.
School of Biomedical Sciences, University of Queensland, Brisbane, Qld 4072, Australia.
Ann E O
AE
Trezise
Carlos M
CM
Farinha
Ghanshyam D
GD
Heda
Ann
A
Harris
Margarida D
MD
Amaral
Nathalie
N
Mouchel
D015152:Blotting, Northern / Q000379:methods; D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells; D005821:Genetic Techniques; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D014158:Transcription, Genetic / Q000235:genetics
C505032:CFTR protein, human; D018076:DNA, Complementary; D019005:Cystic Fibrosis Transmembrane Conductance Regulator