Disentangling ligand migration and heme pocket relaxation in cytochrome P450cam.
In this work we show that ligand migration and active site conformational relaxation can occur independently of each other in hemoproteins. The complicated kinetics of carbon monoxide rebinding with cytochrome P450cam display up to five distinct processes between 77 K and 300 K. They were disentangled by using a combination of three approaches: 1), the competition of the ligand with xenon for the occupation of internal protein cavities; 2), the modulation of the amount of distal steric hindrance within the heme pocket by varying the nature of the substrate; and 3), molecular mechanics calculations to support the proposed heme-substrate relaxation mechanism and to seek internal cavities. In cytochrome P450cam, active site conformational relaxation results from the displacement of the substrate toward the heme center upon photodissociation of the ligand. It is responsible for the long, puzzling bimodal nature of the rebinding kinetics observed down to 77 K. The relaxation rate is strongly substrate-dependent. Ligand migration is slower and is observed only above 135 K. Migration and return rates are independent of the substrate.
Biophysique Moléculaire, Institut Curie, Centre Universitaire, Orsay, France.
Catherine
C
Tetreau
Liliane
L
Mouawad
Samuel
S
Murail
Patricia
P
Duchambon
Yves
Y
Blouquit
Daniel
D
Lavalette
D001665:Binding Sites; D002248:Carbon Monoxide / Q000032:analysis / Q000737:chemistry*; D003198:Computer Simulation; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D006418:Heme / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding
D008024:Ligands; D006418:Heme; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System