Viruses adsorbed on musculoskeletal allografts are inactivated by terminal ethylene oxide disinfection.
In 1987 it was anticipated that unsterilized tissues would transmit virus diseases such as hepatitis and HIV-1 from infected donors so a freeze-drying process for musculoskeletal tissue was developed to include terminal ethylene oxide (EO) exposure for 14 h. We found no studies of EO efficacy when viruses were associated with human allografts so we studied the antiviral effect of terminal EO disinfection using all but the final freeze-drying phase of this clinical processing protocol (CPP). Specifically we looked at EO inactivation of HIV-1, a human hepatitis B surrogate and test viruses known to be highly resistant to disinfecting agents, including irradiation. Freeze-drying, ordinarily required after EO disinfection and part of the CPP, was not done. Suspensions of HIV-1, Bovine viral diarrhea, Reovirus type 3, Duck hepatitis B, Poliomyelitis and Canine parvovirus were adsorbed on glass, demineralized bone powder, and preprocessed strips of femoral cortex, iliac wedges, cancellous blocks and patellar bone-tendon-bone preparations and subjected to EO disinfection. Test viruses were inactivated at the end of 7 h of EO disinfection, providing a safety factor in the CPP of at least 100%. Because allografts can transmit viruses, terminal EO disinfection should provide safer musculoskeletal allografts than non-disinfected tissues or those irradiated with a standard irradiation dose. New spontaneously appearing viruses would probably be inactivated with this terminal EO disinfection but they and viral bioweapons will require individual validation to assure viral inactivation.
University of Southern California School of Medicine, USA. tillmoore@aol.com
Tillman M
TM
Moore
Eli
E
Gendler
El
E
Gendler
D000818:Animals; D001842:Bone and Bones; D001908:Diarrhea Viruses, Bovine Viral / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D005027:Ethylene Oxide / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects; D006801:Humans; D012089:Mammalian orthoreovirus 3 / Q000187:drug effects; D018482:Muscle, Skeletal; D017993:Parvovirus, Canine / Q000187:drug effects; D017955:Poliovirus / Q000187:drug effects; D014184:Transplantation, Homologous
D004202:Disinfectants; D005027:Ethylene Oxide