Fluorescent amplified fragment length polymorphism subtyping of multiresistant Salmonella enterica serovar Typhimurium DT104.
Fluorescent amplified fragment length polymorphism (FAFLP) subtyping analysis was used to genotype multiresistant Salmonella enterica serovar Typhimurium definitive phage type 104. Thirteen distinct FAFLP profiles were found among 85 isolates exhibiting identical pulsed-field gel electrophoresis (PFGE) profiles. A single FAFLP profile was shared by 93% of outbreak-associated isolates and 82% of sporadic isolates. This study demonstrates the value of FAFLP as a high-resolution tool for epidemiological investigation of Salmonella.
Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, London, United Kingdom.
Andrew J
AJ
Lawson
John
J
Stanley
E John
EJ
Threlfall
Meeta
M
Desai
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001434:Bacteriophage Typing; D002417:Cattle; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics