Gene expression characteristic in human auricular cartilage tissue engineering.
This study was to assess collagen type II and collagen type I gene expression in tissue-engineered human auricular: cartilage formed via tissue engineering technique. Large-scale culture expansions were transformed into 3D in vitro construct and were implanted subcutaneously on the dorsal of athymic mice. After 8 weeks, explanted construct was processed in the same manner of native cartilage to facilitate cells for gene expression analysis. Isolated cells from in vivo construct demonstrated expression of type II collagen gene comparable to native cartilage. This study verified that tissue-engineered auricular cartilage expressed cartilage specific gene, collagen type II after in vivo maturation.
Farah Wahida
I
I
Tissue Engineering and Human Genetics Laboratory, Hospital UKM.
Aminuddin
B S
BS
Munirah
S
S
Chua
K H
KH
Fuzina
N H
NH
Isa
M R
MR
Saim
L
L
Ruszymah
B H I
BH
D000199:Actins / Q000235:genetics; D000818:Animals; D002356:Cartilage / Q000637:transplantation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology*; D024042:Collagen Type I / Q000235:genetics*; D024043:Collagen Type II / Q000235:genetics*; D004431:Ear, External; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
D000199:Actins; D024042:Collagen Type I; D024043:Collagen Type II