Solid support membranes for ion channel arrays and sensors: application to rapid screening of pharmacological compounds.
The use of solid supported membranes (SSM) was investigated for reconstitution of ion channels and for potential application to screen pharmacological reagents affecting ion channel function. The voltage-gated Kv1.5 K+ channel was reconstituted on an SSM and a current was measured. This current was dependent on the presence of K+, but not Na+, indicating that the Kv1.5 K+ channel maintained cation specificity when reconstituted on SSM. Two pharmacological reagents applied to Kv1.5 K+ channels reconstituted on SSM had similar inhibitory effects as those measured using Kv1.5 in biological membranes. SSM-mounted ion channels were stable enough to be washed with buffer solution and reused many times, allowing solution exchange essential for pharmacological drug screening.
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA.
Nobunaka
N
Matsuno
Michael
M
Murawsky
James
J
Ridgeway
John
J
Cuppoletti
D000818:Animals; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017743:Equipment Reuse; D007473:Ion Channels / Q000187:drug effects*; D051666:Kv1.5 Potassium Channel; D008567:Membranes, Artificial; D051379:Mice; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000187:drug effects
D007473:Ion Channels; C496982:Kcna5 protein, mouse; D051666:Kv1.5 Potassium Channel; D008567:Membranes, Artificial; D024642:Potassium Channels, Voltage-Gated