Trichomonas vaginalis: random amplified polymorphic DNA analysis of isolates from symptomatic and asymptomatic women in India.
Trichomonas vaginalis, the causative agent for human trichomoniasis, is a protozoan parasite. Trichomoniasis is the most common non-viral sexually transmitted disease. The infection in women may be asymptomatic or may lead to severe vaginitis, cervicitis and severe sequelae. Despite its high prevalence, the genetic variability and factors leading to symptomatic infection have been poorly understood. One thousand women in childbearing age group were screened for the presence of T vaginalis. Thirty-eight women were found positive for T vaginalis and out of these 22 (57.9%) were having symptomatic infection and 16 (42%) were asymptomatic. Fresh isolates from 15 symptomatic and 15 asymptomatic women were axenised and subjected to random amplified polymorphic DNA (RAPD) analysis with the use of five different random primers (OPD 1-OPD 5). The isolates with similar banding pattern were assigned as a single type. OPD 3 indicated least (nine types) while OPD 4 indicated highest typing (18 types) ability. Phylogenetic analysis using RAPD distance software indicated two distinct lineages; upper branch consisting of only seven symptomatic isolates while lower branch consisting of all the 15 asymptomatic isolates the other eight symptomatic isolates were recorded in separate cluster. The study indicated that RAPD technique might be helpful to delineate the pathogenic mechanism(s) for its virulence; however, further studies on large number of isolates are desired to elucidate the findings.
Department of Parasitology and Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Praneet
P
Kaul
Indu
I
Gupta
Rakesh
R
Sehgal
Nancy
N
Malla
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D006801:Humans; D007194:India; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D014247:Trichomonas Vaginitis / Q000469:parasitology / Q000503:physiopathology*; D014246:Trichomonas vaginalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D014621:Vagina / Q000469:parasitology; D014774:Virulence
D016054:DNA, Protozoan