Effect of steady and oscillatory shear stress on F-actin content and distribution in neutrophils.
We investigated neutrophil activation, specifically F-actin content and distribution, in situations mimicking the in vivo environment using steady and oscillatory shear. Under low steady shear (<150 s(-1)) F-actin levels were decreased for both treated (n-formyl-L-methioryl-L-leucyl-L-phenylalanine (fMLP)) and untreated neutrophils. The F-actin content increased with a change to higher steady shear levels. Neutrophils show the same behavior of decreased F-actin content for oscillatory shear (26.7 s(-1)) as they did for steady shear. In both situations, the low shear levels caused a decrease in F-actin content. However, as the magnitude of the shear rate increased, cells showed a reversal to increasing F-actin content. Shear caused a decrease in F-actin in the cell cortex for both control and fMLP treated cells. Ctyochalasin B (CB), a common F-actin assembly blocker, significantly decreased F-actin content. The results indicate that neutrophils regulate their actin network based on the level and type of shear stress they encounter in the bloodstream.
Institute of Biomedical Engineering, West China Center of Medical Sciences, Sichuan University, Chengdu, Sichuan 610041, PR China.
Huai-Qing
HQ
Chen
Wei
W
Tian
Yun-Shuang
YS
Chen
Liang
L
Li
Jeffrey
J
Raum
K-L Paul
KL
Sung
D000199:Actins / Q000032:analysis*; D000704:Analysis of Variance; D005434:Flow Cytometry; D006801:Humans; D018375:Neutrophil Activation; D009504:Neutrophils / Q000502:physiology*; D013314:Stress, Mechanical
D000199:Actins