Blood-cell-specific acetylcholinesterase splice variations under changing stimuli.
Developmental and trauma-induced mechanism(s) that modify inflammation and immune responses in blood cells were recently found to be regulated by acetylcholine. Here, we report corresponding blood cell-specific changes in acetylcholinesterase splice variants. Plasmon resonance and flow cytometry using acetylcholinesterase variant-specific antibody probes, revealed a progressive increase in myeloid cell fractions expressing the apoptosis-related acetylcholinesterase-S variant from newborns to adult controls and post-delivery mothers. Hematopoietic cell fractions positive for the myeloproliferative acetylcholinesterase-R variant, were similarly high in post-partum blood, both intracellular and on the cell surface. Moreover, intracellular acetylcholinesterase-S protein amounts as reflected by fluorescence intensity measurements remained unchanged in myeloid cells from post-partum mothers as compared with matched controls. Unlike brain neurons, which over-express intracellular acetylcholinesterase-R under stress, lymphocytes from post-partum mothers presented increased surface acetylcholinesterase-S and pronounced decreases in both the expression and contents of surface acetylcholinesterase-R. Peripheral stimuli-induced modulations in acetylcholine regulation may hence reflect blood cell lineage-dependent acetylcholinesterase splice variations.
Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Tel Aviv, Israel.
Marjorie
M
Pick
Cesar
C
Flores-Flores
Dan
D
Grisaru
Susana
S
Shochat
Varda
V
Deutsch
Hermona
H
Soreq
D000109:Acetylcholine / Q000097:blood / Q000235:genetics / Q000276:immunology; D000110:Acetylcholinesterase / Q000097:blood* / Q000145:classification / Q000235:genetics / Q000276:immunology*; D000375:Aging / Q000378:metabolism*; D001773:Blood Cells / Q000201:enzymology* / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007231:Infant, Newborn; D018384:Oxidative Stress / Q000276:immunology*; D019154:Protein Splicing / Q000235:genetics
D000110:Acetylcholinesterase; D000109:Acetylcholine