An optimized method for the assay of the red blood cell--age-related enzyme aspartate aminotransferase.
Three methods of preparation of red blood cell concentrate for erythrocyte aspartate aminotransferase measurement were compared: (1) filtration of whole blood through a cellulose column (n = 36); (2) washing of whole blood and aspiration of buffy coat after centrifugation (n = 48); (3) optimized method with washing without aspiration of buffy coat (n = 229).
Robinson
Yohan
Y
Charité, Campus Benjamin Franklin, Institute of Sports Medicine, 12200 Berlin, Germany. yohan.robinson@charite.de
Cristancho
Edgar
E
Böning
Dieter
D
D001219:Aspartate Aminotransferases / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D016922:Cellular Senescence; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D047589:Leukocyte Reduction Procedures / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001219:Aspartate Aminotransferases