Peripherally administered growth hormone increases brain dopaminergic activity and swimming in rainbow trout.
There is increasing evidence that growth hormone (GH) has important behavioral effects in fish, but the underlying mechanisms are not well understood. To investigate if peripherally administered GH influences the monoaminergic activity of the brain, and how this is correlated to behavior, juvenile rainbow trout were implanted intraperitoneally with ovine GH. Fish were either kept isolated or in groups of five. The physical activity and food intake of the isolated fish were observed after 1 and 7 days, when brains were also sampled. The content of serotonin, dopamine, and noradrenaline and their metabolites in hypothalamus, telencephalon, optic tectum, and brain stem was then analyzed. For fish kept isolated for 7 days following implant, GH increased swimming activity and the levels of the dopamine metabolite 3, 4-hydroxy-phenylacetic acid (DOPAC) were higher in all brain parts examined. In the optic tectum, the levels of the dopamine metabolite homovanillic acid (HVA) were lowered by the GH treatment. One-day GH implant did not affect behavior or monoamine levels of isolated fish. In the fish kept in groups, a 7-day GH implant increased the hypothalamic levels of DOPAC, but not in the other brain parts examined, which may indicate an effect on the brain dopaminergic system from social interactions. It can be concluded that peripherally administered GH may function as a neuromodulator, affecting the dopaminergic activity of the rainbow trout brain, and this is associated with increased swimming activity.
Fish Endocrinology Laboratory, Department of Zoology/Zoophysiology, Göteborg University, S-405 30 Göteborg, Sweden. viktoria.johansson@zool.gu.se
Viktoria
V
Johansson
Svante
S
Winberg
Elisabeth
E
Jönsson
David
D
Hall
Björn Thrandur
BT
Björnsson
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D020341:Absorbable Implants; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology; D013006:Growth Hormone / Q000008:administration & dosage / Q000502:physiology*; D007263:Infusions, Parenteral; D009068:Movement / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D011897:Random Allocation; D012701:Serotonin / Q000378:metabolism; D013550:Swimming / Q000502:physiology; D013997:Time Factors
D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D013006:Growth Hormone; D004298:Dopamine; D009638:Norepinephrine