[Changes in the levels of nitric oxide in the mononuclear cells and neutrophils of patients with tuberculosis with varying courses of pulmonary tuberculosis].
A hundred and forty four patients with different types of the course of pulmonary tuberculosis were examined. Before and 20 hours after incubation with BCG-killed cultures, mononuclear cells and neutrophils were used to estimate the level of generation of nitric oxide (NO) from the content of its stabile metabolites--nitrites determined by Griess reagent. Crucially active pulmonary tuberculosis was found to follow the increased leukocytic generation of NO. The most profound shifts occurred in the mononuclear cells. During a favorable involutional process, the mononuclear cells increased their baseline generation of NO and, moreover, preserved their potential for a responsive "nitric oxide burst" when they met a specific causative agent. In patients with acute pulmonary tuberculosis, the basal level of NO in the mononuclear cells was high, but their response to BCG proved to be decreased. In the neutrophils, the basal level of NO exhibited a significant scatter and their higher response to BCG was noted only during the involutional course of tuberculosis. Changes in the leukocytic levels of NO were related to the magnitude of a systemic inflammatory response, to the severity of respiratory failure, and to the rate of cellular lipid peroxidation. A clear relationship was observed between the nature of baseline changes in the values of NA and the efficiency of subsequent treatment.
G O
GO
Kaminskaia
R Iu
RIu
Abdullaev
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism*; D012720:Severity of Illness Index; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000378:metabolism*
D009569:Nitric Oxide