Nitric oxide and effect of a radical scavenger N-tert-butyl-alpha-phenylnitrone on stroke in a rat model.
To characterize the role of nitric oxide (NO) in stroke, NO was measured using an in vivo microdialysis technique and electron spin resonance spectrometry in malignant stroke-prone spontaneously hypertensive rats (M-SHRSP), stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). The brain dialysate NO level was higher in SHRSP than in WKY. NO was not detected in M-SHRSP hippocampus microdialysate after stroke except after the administration of N-tert-butyl-alpha-phenylnitrone (PBN). In addition, very little NO was generated in M-SHRSP brain tissue with hemorrhage. These data demonstrate an association between NO and stroke in M-SHRSP. Further, PBN administration results in maintenance of NO levels after stroke in M-SHRSP.
Institute for Environmental Sciences, University of Shizuoka, Shizuoka, Japan. saitok@smail.u-shizuoka-ken.ac.jp
Kieko
K
Saito
Masahiko
M
Ikeda
Hisashi
H
Yoshioka
Takako
T
Tomita
D000818:Animals; D001921:Brain / Q000378:metabolism; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017551:Microdialysis; D009569:Nitric Oxide / Q000096:biosynthesis*; D009589:Nitrogen Oxides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020521:Stroke / Q000378:metabolism*
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C029217:phenyl-N-tert-butylnitrone