[Effects of 1alpha,25-dihydroxyvitamin D(3) and UVB on cell proliferation and melanin synthesis of cultured human melanocyte].
OBJECTIVE
To study the effects of 1alpha,25-dihydroxyvitamin D(3) and UVB on cell proliferation and melanin synthesis of normal human melanocytes.


METHODS
Melanocytes of foreskins of healthy men were cultured and treated with various concentration of 1alpha,25-dihydroxyvitamin D(3) or UVB (55 mJ/cm(2)),or both. Cell proliferation was measured with MTT assay .The synthesis of melanin was determined by chromatography.


RESULTS
1alpha,25-dihydroxyvitamin D(3) and UVB could promote the proliferation of cultured melanocyte,and 1alpha,25-dihydroxyvitamin D(3) could promote the melanin synthesis. The significant concentration of 1alpha,25-dihydroxyvitamin D(3) ranging from 10(-7) to 10(-10) mol/L.


CONCLUSIONS
1alpha,25-dihydroxyvitamin D(3) and UVB irradiation could promote the proliferation of melanocyte,which indicates that they might be effective in the treatment of vitiligo.
Xu
Qian-xi
QX
Department of Dermatology, Peking University People's Hospital, Beijing 100044, China. xuqianxi@sohu.com
Du
Juan
J
He
Pei-ying
PY
Zhang
Jian-zhong
JZ
Zhu
Tie-jun
TJ
D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
D008543:Melanins; C118756:dihydroxy-vitamin D3; D014807:Vitamin D