Preparation of a tritiated ginkgolide.
Ginkgolide B, a constituent of the tree Ginkgo biloba, was radiolabeled with the beta-emitter tritium ([(3)H]) in two steps from ginkgolide C. First, a triflate precursor was prepared utilizing the selective reactivity of 7-OH in ginkgolide C; the triflate was then reduced with sodium borotritide to yield tritiated ginkgolide B ([(3)H]GB) in good yield and high specific activity. The tritiated ginkgolide will be an important tool for studying neuromodulatory properties of ginkgolides.
Department of Chemistry, Columbia University, New York, NY 10027, USA.
Kristian
K
Strømgaard
Makiko
M
Suehiro
Koji
K
Nakanishi
D004224:Diterpenes / Q000138:chemical synthesis* / Q000737:chemistry; D020441:Ginkgo biloba / Q000737:chemistry; D046934:Ginkgolides; D007783:Lactones / Q000138:chemical synthesis* / Q000737:chemistry; D008968:Molecular Conformation; D014316:Tritium / Q000737:chemistry*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D014316:Tritium; C058295:ginkgolide C; C045856:ginkgolide B