Cloning and functional analysis by gene disruption of a novel gene involved in indigo production and fluoranthene metabolism in Pseudomonas alcaligenes PA-10.
A novel indole dioxygenase (idoA) gene has been cloned from Pseudomonas alcaligenes PA-10, based on its ability to convert indole to indigo. The chromosomally encoded idoA gene exhibits no similarity to previously cloned naphthalene dioxygenases or to aromatic oxygenases from other species at the nucleotide level. Phylogenetic analysis indicates that the idoA gene product is most similar to an acyl-CoA dehydrogenase from Novosphingobium aromaticivorans. The enzyme encoded by the idoA gene is essential for the metabolism of fluoranthene, since a mutant in which the idoA gene has been disrupted looses the ability to degrade this compound. The idoA gene appears to be constitutively expressed in PA-10, but its expression is also subject to regulation following prior exposure to salicylate and to fluoranthene degradative intermediates.
Department of Microbiology, University College Cork, National University of Ireland, Cork, Ireland.
D
D
Alemayehu
L M
LM
Gordon
M M
MM
O'Mahony
N D
ND
O'Leary
A D W
AD
Dobson
D003001:Cloning, Molecular; D049308:Dioxygenases / Q000378:metabolism; D005449:Fluorenes / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism*; D010802:Phylogeny; D044202:Pseudomonas alcaligenes / Q000235:genetics* / Q000378:metabolism
D005449:Fluorenes; D007211:Indoles; C007738:fluoranthene; D007203:Indigo Carmine; D049308:Dioxygenases; C496045:indole dioxygenase idoA, Pseudomonas alcaligenes