[Preparation and application of monoclonal antibodies against cowpea trypsin inhibitor].
OBJECTIVE
To detect cowpea trypsin inhibitor (CpTI) in transgenic rice leaves and build a possible method to assay transgenic plant with CpTI.


METHODS
Nowadays gene assay is the most useful method to detect transgenic plants. But false positive may be got in gene assay and effective protein may not be produced. So assay of foreign protein is the most direct and conformable method. Monoclonal antibodies were got through traditional method of preparation.


RESULTS
Three monoclonal antibodies were got. Detection of CpTI in transgenic rice leaves with these three antibodies mixes through Western blotting was conducted and the result was satisfying.


CONCLUSIONS
The method of using the mixes of three antibodies to detect the CpTI in plants through Western blotting was satisfying.
National Institute For Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing.
Xiaoping
X
Chen
Lichen
L
Yang
Xiaoguang
X
Yang
Jianhua
J
Piao
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000911:Antibodies, Monoclonal; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases