[Localization of matrix-associated elements in the 5'-region of the rat estrogen sulfotransferase gene].
Potential matrix-associated elements in the 5'-region of the rat estrogen sulfotransferase gene (Ste1) were searched for and characterized. The DNA fragments corresponding to the regions -800/+1048 and +1049/+2038 relative to the main point of transcription initiation were found to be bound to the nuclear matrix in vitro. A permanent association of the 5'-region of the Ste1 gene with the nuclear matrix in rat hepatocytes was found, the most probable site of attachment being the region -352/-152. No differences were found in the attachment of the 5'-region of the Ste1 gene to the nuclear matrix in the liver of males where the gene is actively transcribed and in that of females where it is inactive.
I A
IA
Morozov
I I
II
Astapova
A N
AN
Smirnov
P M
PM
Rubtsov
D024506:5' Flanking Region / Q000235:genetics*; D000818:Animals; D002522:Chlorocebus aethiops; D005260:Female; D022781:Hepatocytes / Q000201:enzymology; D008297:Male; D015530:Nuclear Matrix / Q000201:enzymology / Q000235:genetics*; D051381:Rats; D012737:Sex Factors; D015238:Sulfotransferases / Q000235:genetics* / Q000378:metabolism
D015238:Sulfotransferases; C020122:estrone sulfotransferase