Homeodomain-leucine zipper proteins interact with a plant homologue of the transcriptional co-activator multiprotein bridging factor 1.
StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. In an attempt to understand the role of StMBF1, we analyzed its interaction with plant transcription factors of the homeodomain-leucine zipper (Hd-Zip) family, a group of proteins with a typical leucine zipper motif adjacent to a homeodomain. StMBF1 is able to interact in vitro with the Hd-Zip protein Hahb-4 both in the presence and absence of DNA. Upon binding, StMBF1 increases the DNA binding affinity of Hahb-4, and of another plant homeodomain containing protein from the GL2/Hd-Zip IV family, HAHR-1. The biological role of interactions is discussed in this paper.
Instituto de Investigaciones Biológicas, Departamento de Biología, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina.
María Eugenia
ME
Zanetti
Raquel L
RL
Chan
Andrea V
AV
Godoy
Daniel H
DH
González
Claudia A
CA
Casalongué
D000818:Animals; D004247:DNA / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016350:Leucine Zippers; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D018398:Homeodomain Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D004247:DNA