The genome sequence analysis of H5N1 avian influenza A virus isolated from the outbreak among poultry populations in Thailand.
In this report, the genome of the Thai avian influenza virus A (H5N1); A/Chicken/Nakorn-Pathom/Thailand/CU-K2/04, isolated from the Thai avian influenza A (AI) epidemic during the early of 2004 was sequenced. Phylogenetic analyses were performed in comparison to AI viruses from Hong Kong 1997 outbreaks and other AI (H5N1) isolates reported during 2001-2004. Molecular characterization of the Thai AI (H5N1) HA gene revealed a common characteristic of a highly pathogenic AI (HPAI), a 20-codon deletion in the neuraminidase gene, a 5-codon deletion in the NS gene and polymorphisms of the M2 and PB2 genes. Moreover, the HA and NA genes of the Thai AI displayed high similarity to those of the AI viruses isolated from human cases during the same epidemic. Finally, our results demonstrated that the Thai AI emerged as a member of 2000's AI lineage with most of the genetic sequences closely related to the Influenza A/Duck/China/E319.2/03 (H5N1).
Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Nareerat
N
Viseshakul
Roongroje
R
Thanawongnuwech
Alongkorn
A
Amonsin
Sanipa
S
Suradhat
Sunchai
S
Payungporn
Juthatip
J
Keawchareon
Kanisak
K
Oraveerakul
Piya
P
Wongyanin
Sukanya
S
Plitkul
Apiradee
A
Theamboonlers
Yong
Y
Poovorawan
D000595:Amino Acid Sequence; D000818:Animals; D003062:Codon; D004196:Disease Outbreaks / Q000662:veterinary*; D017353:Gene Deletion; D016679:Genome, Viral; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011200:Poultry; D016415:Sequence Alignment; D013785:Thailand / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
D003062:Codon; D014764:Viral Proteins