Adsorption of bile acid by chitosan-orotic acid salt and its application as an oral preparation.
The orotic acid (OT) salt of chitosan (CS), CS-OT, and that of a CS derivative, CP, were prepared, and the adsorption of primary or secondary bile acid was investigated. Calcium-induced alginate gel beads (Alg-Ca) containing CS-OT were also prepared and autoclaved, and the possibility of these beads to act as a vehicle for oral administration to prevent hyperlipidemia was investigated. When taurocholate (TCA) and glycocholate (GCA) were present together in the medium, CS-OT adsorbed identical amounts of both bile acids. This trend was seen in all CPs, although the capacity to adsorb bile acid was affected by the number and/or structure of the amino groups in the CP. On the other hand, taurodeoxycholate, a secondary bile acid was preferentially adsorbed over TCA and GCA. Alg-Ca containing CS-OT took up bile acids in a similar manner as CS-OT irrespective of the water content of the gel matrix. As all elements can be taken as a food, Alg-Ca containing CS-OT could serve as a useful dietary agent for the prevention of hyperlipidemia, which is a lifestyle-related disease.
Faculty of Pharmaceutical Science, Hokuriku University, Kanazawa, Japan. y-murata@hokuriku-u.ac.jp
Yoshifumi
Y
Murata
Sayuri
S
Kudo
Kyouko
K
Kofuji
Etsuko
E
Miyamoto
Susumu
S
Kawashima
D000284:Administration, Oral; D000327:Adsorption; D000464:Alginates / Q000737:chemistry; D001647:Bile Acids and Salts / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004304:Dosage Forms; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009963:Orotic Acid / Q000008:administration & dosage / Q000737:chemistry*
D000464:Alginates; D001647:Bile Acids and Salts; D004304:Dosage Forms; D006603:Hexuronic Acids; D009963:Orotic Acid; D020723:Glucuronic Acid; D048271:Chitosan