Enhancement of pheromone response by RGS9 and Gbeta5 in yeast.
The G-protein gamma-subunit-like (GGL) domain present within a subfamily of RGS proteins binds specifically to Gbeta5. This interaction and resulting biological effect impacts the standard model of heterotrimeric G-protein signaling. It has been hypothesized that the RGS/Gbeta5 may potentially substitute for Gbetagamma in the heterotrimeric complex. Saccharomyces cerevisiae pheromone responsive mating signaling pathway is primarily driven by Gbetagamma. We evaluated GGL containing RGS9 and RGS7 for functional complementation in a RGS (sst2Delta) knockout yeast strain. The potential of Gbeta5 to augment the function of these RGS proteins was also evaluated. While Gbeta5 had no effect on RGS7, coexpression of Gbeta5 with RGS9 enhanced cell cycle arrest, suggesting that under certain conditions, RGS9 and Gbeta5 may possibly function as betagamma dimer. Furthermore, we demonstrate that Gbeta5 can complement a ste4Delta, the yeast beta-subunit, thus providing the first evidence of functional complementation of a mammalian Gbeta.
Ajit
Seena K
SK
Neuroscience Discovery Research, Wyeth Research, Princeton, NJ 08543, USA.
Young
Kathleen H
KH
D015153:Blotting, Western; D002453:Cell Cycle; D018076:DNA, Complementary / Q000378:metabolism; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism*; D005816:Genetic Complementation Test; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D010675:Pheromones / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D020710:RGS Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction
D018076:DNA, Complementary; C490611:GNB5 protein, human; D044387:GTP-Binding Protein beta Subunits; D010675:Pheromones; D020710:RGS Proteins; D011994:Recombinant Proteins; C116925:regulator of g-protein signaling 9; D020962:Heterotrimeric GTP-Binding Proteins