Acetylsalicylic acid inhibits ATP diphosphohydrolase activity by platelets from adult rats.
METHODS
The in vitro effect of the nonsteroidal anti-inflammatory drug, acetylsalicylic acid (ASA), on the extracellular adenine nucleotide hydrolysis by intact rat blood platelets was studied.


RESULTS
Our results demonstrate that aspirin, at final concentrations of 2.0 and 3.0 mM, inhibits ATP extracellular hydrolysis in vitro by approximately 17% and 21%, respectively. Aspirin, at a final concentration of 3.0 mM, also inhibited in vitro extracellular ADP hydrolysis by approximately 41%. The same concentrations of this drug, however, did not alter AMP hydrolysis by intact rat blood platelets under similar assay conditions. The kinetic analysis demonstrated that the inhibition of ADP and ATP hydrolysis by aspirin in rat platelets is of the uncompetitive type.


CONCLUSIONS
In this study, we demonstrated an inhibitory effect of ASA upon E-NTPDase 3 activity of platelets from adult rats and discussed the significance of our findings.
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, ANEXO, CEP Porto Alegre, RS, Brazil. debuffon@redemeta.com.br
Andréia
A
Buffon
Vanessa B
VB
Ribeiro
Cristina R
CR
Fürstenau
Ana M O
AM
Battastini
João J F
JJ
Sarkis
D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors; D000227:Adenine Nucleotides / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D001081:Apyrase / Q000037:antagonists & inhibitors* / Q000097:blood*; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D012394:Rosaniline Dyes
D000227:Adenine Nucleotides; D016861:Cyclooxygenase Inhibitors; D012394:Rosaniline Dyes; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; C048139:Coomassie blue; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D001081:Apyrase; D001241:Aspirin