Peri/nuclear localization of intact insulin-like growth factor binding protein-2 and a distinct carboxyl-terminal IGFBP-2 fragment in vivo.
Insulin-like growth factor binding protein-2 (IGFBP-2) as one of the most important IGFBPs has never been assessed in the intracellular compartment in vivo. Since there is evidence for novel intracellular functions of distinct IGFBPs, we investigated the presence of IGFBP-2 inside the cell. In peri/nuclear fractions of various tissues isolated from IGFBP-2 transgenic and non-transgenic mice we were able to show the presence of intact IGFBP-2. In addition, we demonstrate the presence of a highly conserved carboxyl-terminal IGFBP-2 fragment in the peri/nuclear fraction by using different peptide-induced antibodies. In pancreatic sections, confocal microscopy revealed the presence of IGFBP-2 on the nuclear surface but not within the nucleus. Our findings suggest novel functions of intact IGFBP-2 and IGFBP-2 fragments within the cell.
Institute of Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilians University, 81377 Munich, Germany. hoeflich@lmb.uni-muenchen.de
A
A
Hoeflich
R
R
Reisinger
B S
BS
Schuett
M W
MW
Elmlinger
V C
VC
Russo
G A
GA
Vargas
P M
PM
Jehle
H
H
Lahm
I
I
Renner-Müller
E
E
Wolf
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D047468:Immunoprecipitation; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011419:Propidium / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018971:Insulin-Like Growth Factor Binding Protein 2; D008024:Ligands; D010455:Peptides; D011419:Propidium