Isolation of lightning-competent soil bacteria.
Artificial transformation is typically performed in the laboratory by using either a chemical (CaCl(2)) or an electrical (electroporation) method. However, laboratory-scale lightning has been shown recently to electrotransform Escherichia coli strain DH10B in soil. In this paper, we report on the isolation of two "lightning-competent" soil bacteria after direct electroporation of the Nycodenz bacterial ring extracted from prairie soil in the presence of the pBHCRec plasmid (Tc(r), Sp(r), Sm(r)). The electrotransformability of the isolated bacteria was measured both in vitro (by electroporation cuvette) and in situ (by lightning in soil microcosm) and then compared to those of E. coli DH10B and Pseudomonas fluorescens C7R12. The electrotransformation frequencies measured reached 10(-3) to 10(-4) by electroporation and 10(-4) to 10(-5) by simulated lightning, while no transformation was observed in the absence of electrical current. Two of the isolated lightning-competent soil bacteria were identified as Pseudomonas sp. strains.
Cérémonie
Hélène
H
Ecologie Microbienne UMR-CNRS 5557, Université Claude Bernard Lyon 1, BAt. G. Mendel, 43 Blvd. du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
Buret
François
F
Simonet
Pascal
P
Vogel
Timothy M
TM
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D018274:Electroporation; D005798:Genes, Bacterial; D008030:Lightning; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D012988:Soil Microbiology; D014170:Transformation, Genetic