[TK locus mutation assay: comparison of L5178Y and TK6 cell lines].
OBJECTIVE
To compare the advantages and disadvantages of utilizing L5178Y and TK6 cell lines in the TK locus mutation assay.


METHODS
The two cell lines were used for detecting and assessing the mutability of four chemicals (MMS, EMS, MMC and KCl); the microwell method of TK locus mutation assay was adopted.


RESULTS
The two cell lines brought about similar results in the study. The tolerance of TK6 to all four chemicals was lower than that of L5178Y, and all the relative mutation indices of TK6 were higher than those of L5178Y.


CONCLUSIONS
Each of the two cell lines has its own strong points; nevertheless, the authors recommend the applications of TK6 cell in the assay system since this cell line comes from human.
Shuai
Pei-qiang
PQ
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan, University, Chengdu 610041, China.
Zhang
Li-shi
LS
Wang
Yi-jing
YJ
D000818:Animals; D045744:Cell Line, Tumor; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D006801:Humans; D007940:Leukemia L5178 / Q000201:enzymology* / Q000235:genetics; D008223:Lymphoma / Q000201:enzymology* / Q000235:genetics; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D009154:Mutation; D013937:Thymidine Kinase / Q000235:genetics*
D016685:Mitomycin; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D013937:Thymidine Kinase