Diagnosis of candidosis by amplification of small subunit of 18S rRNA gene.
A PCR assay for the diagnosis of infection produced by Candida sp. was developed. The primers, designated 520 and 522, were selected from highly-conserved areas of the small subunit (ssu) 18S rRNA gene of Candida spp. To check the value of the results a Candida albicans oligonucleotide probe, digoxigenin-labeled, and a general Candida probe were used in hybridization experiments with the amplified products. We were able to detect a Candida- specific fragment of 1800 bp from different clinical samples. The procedure described could provide an interesting complement to present diagnostic methods of detecting Candida sp in clinical samples.
Servicio de Microbiología, Hospital de Basurto, Avda. Montevideo 18, 48013-Bilbao, Bizkaia, España.
M J
MJ
Unzaga
L
L
Gallego
P
P
Berdonces
J R
JR
Dones
R
R
Cisterna