Induction of anti-leukemic cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous myeloblasts.
Presentation of AML antigens by dendritic cells (DC) could potentially induce a T cell-mediated anti-leukemic immune response. In the present study, we generated DC from adherent (AD-DC) and non-adherent (NAD-DC) myeloblasts obtained from bone marrows of AML patients. Both cell populations displayed morphological, phenotypic and functional properties of DC. The functions of NAD-DC were compared to AD-DC that had been fused with autologous AML blasts (FC/AML). The FC/AML induced greater T cell proliferation and CTL activity against autologous AML blasts (9/10 cases) as compared to NAD-DC. FC/AML may thus represent a promising strategy for DC-based immunotherapy of patients with AML.
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. jgong@medicine.bu.edu
Jianlin
J
Gong
Shigeo
S
Koido
Yoko
Y
Kato
Yasuhiro
Y
Tanaka
Dongshu
D
Chen
Anna
A
Jonas
Ilene
I
Galinsky
Daniel
D
DeAngelo
David
D
Avigan
Donald
D
Kufe
Richard
R
Stone
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000473:pathology*; D005260:Female; D042381:Granulocyte Precursor Cells / Q000473:pathology*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*