A first version of the Caenorhabditis elegans Promoterome.
An important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we address one of the challenges of C. elegans localizome mapping projects: that of obtaining a genome-wide resource of C. elegans promoters needed to generate transgenic animals expressing localization markers such as the green fluorescent protein (GFP). To ensure high flexibility for future uses, we utilized the newly developed MultiSite Gateway system. We generated and validated "version 1.1" of the Promoterome: a resource of approximately 6000 C. elegans promoters. These promoters can be transferred easily into various Gateway Destination vectors to drive expression of markers such as GFP, alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).
Dupuy
Denis
D
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Li
Qian-Ru
QR
Deplancke
Bart
B
Boxem
Mike
M
Hao
Tong
T
Lamesch
Philippe
P
Sequerra
Reynaldo
R
Bosak
Stephanie
S
Doucette-Stamm
Lynn
L
Hope
Ian A
IA
Hill
David E
DE
Walhout
Albertha J M
AJ
Vidal
Marc
M
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D016366:Open Reading Frames / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*
D008164:Luminescent Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins