Global analysis of host cell gene expression late during cytomegalovirus infection reveals extensive dysregulation of cell cycle gene expression and induction of Pseudomitosis independent of US28 function.
Replication of human cytomegalovirus (CMV) depends on host cell gene products working in conjunction with viral functions and leads to a dramatic dysregulation of cell cycle gene expression. Comprehensive transcriptional profiling was used to identify pathways most dramatically modulated by CMV at late times during infection and to determine the extent to which expression of the viral chemokine receptor US28 contributed to modulating cellular gene expression. Cells infected with the AD169 strain of virus or a fully replication competent US28-deficient derivative (RV101) were profiled throughout the late phase of infection (50, 72, and 98 h postinfection). Although sensitive statistical analysis showed striking global changes in transcript levels in infected cells compared to uninfected cells, the expression of US28 did not contribute to these alterations. CMV infection resulted in lower levels of transcripts encoding cytoskeletal, extracellular matrix, and adhesion proteins, together with small GTPases and apoptosis regulators, and in higher levels of transcripts encoding cell cycle, DNA replication, energy production, and inflammation-related gene products. Surprisingly, a large number of cellular transcripts encoding mitosis-related proteins were upmodulated at late times in infection, and these were associated with the formation of abnormal mitotic spindles and the appearance of pseudomitotic cells. These data extend our understanding of how broadly CMV alters the regulation of host cell cycle gene products and highlight the establishment of a mitosis-like environment in the absence of cellular DNA replication as important for viral replication and maturation.
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5124, USA.
Laura
L
Hertel
Edward S
ES
Mocarski
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D004261:DNA Replication; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D018816:Genes, cdc; D006801:Humans; D016023:Integrins / Q000235:genetics; D045726:Metalloproteases / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism; D008938:Mitosis; D019707:Receptors, Chemokine / Q000032:analysis / Q000502:physiology*; D013997:Time Factors; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
D016326:Extracellular Matrix Proteins; D016023:Integrins; D019707:Receptors, Chemokine; C411191:US28 receptor, Cytomegalovirus; D014764:Viral Proteins; D045726:Metalloproteases