RNA is more UV resistant than DNA: the formation of UV-induced DNA lesions is strongly sequence and conformation dependent.
DNA and RNA hairpins, which represent well-folded oligonucleotide structures, were irradiated and the amount of damaged hairpins was directly quantified by using ion-exchange HPLC. The types of photoproducts formed in the hairpins were determined by ESI-HPLC-MS/MS experiments. Irradiation of hairpins with systematically varied sequences and conformations (A versus B) revealed remarkable differences regarding the amount of photolesions formed. UV-damage formation is, therefore, a strongly sequence and conformation dependent process.
Department Chemie, Ludwig-Maximilians University, Butenandtstrasse 5-13, 81377 Munich, Germany.
Lal Mohan
LM
Kundu
Uwe
U
Linne
Mohamed
M
Marahiel
Thomas
T
Carell
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D013058:Mass Spectrometry; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D012313:RNA / Q000737:chemistry / Q000528:radiation effects*; D014466:Ultraviolet Rays
D012313:RNA; D004247:DNA