Induction of VEGF and its Flt-1 receptor after sciatic nerve crush injury.
Expression of vascular endothelial growth factor and its receptors Flt-1 and Flk-1 was studied in lumbar spinal cord after sciatic nerve crush injury. Immunohistochemical staining revealed strikingly different distribution of VEGF, Flt-1, and Flk-1 in lumbar motor neurons. VEGF was observed both in the nuclei and perikarya, while Flk-1 had cytoplasmic and Flt-1 perinuclear localization. Real-time RT-PCR showed a significant increase in the expression of VEGF and Flt-1 on the injured side of the lumbar spinal cord. The increased level of VEGF was also detected by immunoblot. Here we show that lumbar motor neurons increase the expression of VEGF and Flt-1 in response to injury. We propose that VEGF/Flt-1 signaling may be involved in regeneration of the spinal motor neurons.
Department of Physiology, The Brody School of Medicine, East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA.
Rustem R
RR
Islamov
Vishnu
V
Chintalgattu
Elena S
ES
Pak
Laxmansa C
LC
Katwa
Alexander K
AK
Murashov
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D007839:Functional Laterality / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D018995:Myosin Heavy Chains; D009409:Nerve Crush / Q000379:methods; D024501:Nonmuscle Myosin Type IIB; D012333:RNA, Messenger / Q000096:biosynthesis; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D020426:Sciatic Neuropathy / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D002795:Choline O-Acetyltransferase; C501163:Flt1 protein, mouse; D040262:Receptors, Vascular Endothelial Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains