[Inter-laboratory evaluation studies for development of notified ELISA methods for allergic substances (milk)].
Extracts of sausage, sauce, cookie, cereal and pasta sauce spiked with milk standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of the three ELISA methods using a Milk Protein Casein ELISA Kit (Casein kit), a Milk Protein Beta-Lactoglobulin ELISA Kit (Beta-Lactoglobulin kit) and a FASTKIT Milk ELISA Kit (Milk ELISA kit) were mostly below 10%. Mean recoveries of the milk standard protein from the food extracts were over 40% in the three ELISA methods with a few excertions. The recoveries of milk standard protein from the sauce extract in Casein kit were improved by adjusting the extract to neutrality before the Casein kit assay. The recoveries of milk standard protein from cookie, cereal and pasta sauce were improved by the increasing the amount of antibody coated in the Milk ELISA kit. The detection limits of all the ELISA methods were 1 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of milk protein levels in extracts of sausage, sauce, cookie, cereal and pasta sauce.
National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Hiroshi
H
Akiyama
Kazuto
K
Isuzugawa
Naoki
N
Harikai
Hiroko
H
Watanabe
Ken
K
Iijima
Hirohito
H
Yamakawa
Yamato
Y
Mizuguchi
Reiji
R
Yoshikawa
Miho
M
Yamamoto
Hidetaka
H
Sato
Masatoshi
M
Watai
Fumihiro
F
Arakawa
Takeshi
T
Ogasawara
Rikuka
R
Nishihara
Hisashi
H
Kato
Atsushi
A
Yamauchi
Yoshihisa
Y
Takahata
Fumiki
F
Morimatsu
Shinichi
S
Mamegoshi
Shiroo
S
Muraoka
Tsutomu
T
Honjoh
Takahiro
T
Watanabe
Kozue
K
Sakata
Tomoaki
T
Imamura
Masatake
M
Toyoda
Rieko
R
Matsuda
Tamio
T
Maitani
D000485:Allergens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008894:Milk Proteins / Q000032:analysis*; D015337:Multicenter Studies as Topic; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
D000485:Allergens; D008894:Milk Proteins; D011933:Reagent Kits, Diagnostic