[The influence of protein kinase C inhibitor in eosinophil apoptosis of nasal polyps].
OBJECTIVE
To study the influence of the inhibitor of protein kinase C (PKC), Iso-H7 [1-(5-lsoquinolinyl-sulfony)-3-methylpiperazine dihydrochloride] on eosinophil apoptosis in nasal polyp tissues.


METHODS
Nasal polyp tissues were cultured and divided into two groups, test group (with Iso-H7 in different concentration) and control group (without the inhibitor of PKC), to check out the influence of Iso-H7 on eosinophil apoptosis in nasal polyps. The nasal polyp tissues were cultured in Iso-H7 in concentration of 10(-6), 10(-4), 10(-2) mol/L for 1 d, 3 d, and 5 d, respectively. Eosinophil apoptosis index was measured with TUNEL (terminal-deoxynucleotidyl transferase d-UTP nick end labeling), in situ hybridization and MGG staining to identify eosinophil apoptosis.


RESULTS
Occasionally, there was little eosinophil apoptosis in two groups in 1 d, but in 3 d, There were a great deal of eosinophil apoptosis in test group. The eosinophil apoptosis indexs of the two groups were significantly different (P < 0.01). The indexs of eosinophil apoptosis was not significantly higher in 3 d than that in 5 d in test groups (P > 0. 05), but significantly higher than that in 1d (P < 0. 01).


CONCLUSIONS
Iso-H7 takes a important role in accelerating eosinophil apoptosis. The inhibitor of PKC could significantly promote eosinophil apoptosis in nasal polyps. There is no dosage and time dependence of PKC inhibitor in regulating eosinophil apoptosis of nasal polyps.
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031, China. meihl@hotmail.com
Mei-Hua
MH
Li
Zhan-Quan
ZQ
Yang
Wan-Zhong
WZ
Yin
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004804:Eosinophils / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000473:pathology*; D011493:Protein Kinase C / Q000378:metabolism
D004791:Enzyme Inhibitors; C080501:1-(5-isoquinolinylsulfonyl)-3-methylpiperazine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C