Gene and protein profiling of the response of MA-10 Leydig tumor cells to human chorionic gonadotropin.
Activation of the steroidogenic machinery by peptide hormones involves a number of steps for transmitting signals from the plasma membrane to mitochondria in a spatially and temporally coordinated manner. Although key proteins mediating the hormonal signal have been identified, recent data suggest that the pathway might involve more complex protein-protein and protein-lipid interactions. Genomic and proteomic methods of analysis, namely the Affymetrix Murine Genome U74A v2 GeneChip and the BD PowerBlot Western Array, were used to identify human chorionic gonadotropin (hCG)-induced changes in mRNA and protein of MA-10 Leydig tumor cells that parallel the increase seen in progesterone synthesis. To analyze the massive amount of data that was generated, a comprehensive protein information matrix summarizing the features of each gene or protein, including its known properties, as well as annotations derived by homology-based functional inference, was developed. Of the genes examined by Affymetrix array, approximately 79 were differentially expressed and of gene products examined by PowerBlot, 9 were differentially expressed (above twofold). Changes in the expression of selected transcripts of interest were confirmed using real-time quantitative polymerase chain reaction and immunoblot analyses. Collectively, these results indicate that hormonal regulation of steroidogenesis is a complex phenomenon, involving proteins that participate in various known and novel pathways, which are implicated in transmitting signals from the plasma membrane to mitochondria and nucleus.
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Basic Sciences Bldg, 3900 Reservoir Rd, Washington, DC 20057, USA.
Wenping
W
Li
Hakima
H
Amri
Hongzhan
H
Huang
Cathy
C
Wu
Vassilios
V
Papadopoulos
D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D019295:Computational Biology; D020869:Gene Expression Profiling; D006801:Humans; D015151:Immunoblotting; D016247:Information Storage and Retrieval; D007984:Leydig Cell Tumor / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D011374:Progesterone / Q000096:biosynthesis; D040081:Protein Array Analysis; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology
D006063:Chorionic Gonadotropin; D011374:Progesterone