Rat-PET study without anesthesia: anesthetics modify the dopamine D1 receptor binding in rat brain.
Positron emission tomography (PET) measurements in 6-month-old F344/N rats were performed in the conscious state and the influence of chloral hydrate, ketamine, and pentobarbital anesthesia on dopamine D(1) (DA-D(1)) receptor binding was evaluated using [(11)C]SCH23390, a selective DA-D(1) receptor ligand. To perform the PET study in conscious rats, an original fixation apparatus was developed and the animals were trained to acclimate to the scanning atmosphere for 3 h. This training was carried out twice a day for 2 weeks. PET measurements in conscious rats were successful, since the trained rats scarcely moved during the scanning (as monitored by video camera) and since highly reproducible measurements of binding potential (BP) were derived from their scanning. Chloral hydrate and ketamine anesthesia significantly increased the striatal BP of DA-D(1) receptors by 36% and 46%, respectively, compared to that observed in the conscious state. In contrast, pentobarbital markedly decreased the BP by 41%. These BP values of DA-D(1) receptors were calculated using a curve-fitting method. Our results indicate that PET studies in rats should be performed in the conscious state since the anesthetics dramatically modified ligand-receptor bindings, such as DA-D(1) receptor binding, in rat brain.
Department of Brain Science and Molecular Imaging, National Institute for Longevity Sciences, Aichi 474-8522, Japan. momosaki@nils.go.jp
Sotaro
S
Momosaki
Kentaro
K
Hatano
Yasuhiro
Y
Kawasumi
Takashi
T
Kato
Rie
R
Hosoi
Kaoru
K
Kobayashi
Osamu
O
Inoue
Kengo
K
Ito
D000758:Anesthesia; D000818:Animals; D001552:Benzazepines / Q000378:metabolism / Q000494:pharmacology; D001921:Brain / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000502:physiology; D002250:Carbon Radioisotopes; D003213:Conditioning, Psychological; D003243:Consciousness; D003345:Corticosterone / Q000097:blood; D018492:Dopamine Antagonists / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D049268:Positron-Emission Tomography / Q000379:methods*; D051381:Rats; D011916:Rats, Inbred F344; D017447:Receptors, Dopamine D1 / Q000378:metabolism*
D001552:Benzazepines; D002250:Carbon Radioisotopes; D018492:Dopamine Antagonists; D017447:Receptors, Dopamine D1; D003345:Corticosterone