[DNA binding activity of nuclear factor-kappa B in human astrocytoma].
OBJECTIVE
Transcription factors of human cancer cells can regulate the expression of many malignancy-related gene products,relate to poor differentiation,and high metastasis of cancer cells,and also inhibit apoptosis of cancer cells. This study was designed to investigate the nuclear factor-kappa B (NF-kappaB)DNA binding activity in human astrocytoma tissue,and its significance with human astrocytoma development.


METHODS
The NF-kappaB DNA binding activities of 12 cases of normal brain tissue, and 37 cases of human astrocytoma tissue were detected by electrophoretic mobility shift assay (EMSA), and photo densitometry.


RESULTS
The absorbency of NF-kappaB DNA binding activity in human astrocytoma tissue was 134.2+/-24.1, higher than that in normal brain tissue (97.5+/-1.9)(P< 0.05). The NF-kappaB DNA binding activity in multiform glioblastoma tissue was highest (106.8+/-7.4), that in anaplasia Astrocytoma was 123.2+/-10.1, in astroblastoma was 139.3+/-16.8,and in astrocytoma was 160.2+/-18.6 (P< 0.05).


CONCLUSIONS
The NF-kappaB DNA binding activity correlates with malignancy degree of human astrocytoma.
Laboratory of Respiratory, Pediatric Hospital, Fudan University, Shanghai, 200032, P.R.China. guochunbao@hotmail.com
Chun-Bao
CB
Guo
Feng
F
Chen
Zong-Wei
ZW
Gao
D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D005909:Glioblastoma / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D011485:Protein Binding
D004273:DNA, Neoplasm; D016328:NF-kappa B