Novel fluo-4 analogs for fluorescent calcium measurements.
We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.
Martin
Vladimir V
VV
Molecular Probes Invitrogen Detection Technologies, 29851 Willow Creek Rd, Eugene, OR 97492, USA.
Beierlein
Michael
M
Morgan
Josh L
JL
Rothe
Anca
A
Gee
Kyle R
KR
D000814:Aniline Compounds / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002118:Calcium / Q000032:analysis*; D002531:Cerebellum / Q000737:chemistry; D002645:Chickens; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D012165:Retinal Ganglion Cells / Q000737:chemistry; D014966:Xanthenes / Q000032:analysis* / Q000737:chemistry
D000814:Aniline Compounds; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D002118:Calcium