Astrocytic and neuronal biochemical markers in the sera of subjects with diabetes mellitus.
To determine if clinical diabetes is associated with disruption of the blood-brain barrier (BBB) and/or brain injury, enzyme-linked immunoassays and Western blots were used to measure serum levels of S100B, NSE and their auto-antibodies in type 1 and type 2 diabetic subjects. Serum S100B concentrations in type 2 diabetic subjects, but not in type 1 diabetic subjects, were significantly lower than those found in healthy controls. There were no significant differences in serum NSE levels of either type 1 or type 2 diabetics compared to healthy controls. However, there was a significant increase in antibodies to NSE in both type 1 and type 2 diabetic subjects compared to controls, whereas diabetics and controls had equally very low levels of anti S100B auto-antibodies. These studies suggest that diabetes in humans may be associated with alterations in the BBB integrity that allow the emergence of antibodies against neuronal antigens.
Hovsepyan
Meri R
MR
Department of Internal Medicine, Saint Louis University Medical School, Saint Louis, MO 63104, USA. mery@mb.sci.am
Haas
Michael J
MJ
Boyajyan
Anna S
AS
Guevorkyan
Astghik A
AA
Mamikonyan
Ashot A
AA
Myers
Susan E
SE
Mooradian
Arshag D
AD
D000293:Adolescent; D000328:Adult; D000368:Aged; D001253:Astrocytes / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western / Q000379:methods; D002648:Child; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000097:blood*; D009474:Neurons / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*
D015415:Biomarkers; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; D010751:Phosphopyruvate Hydratase