Control of a multisubunit DNA motor by a thermoresponsive polymer switch.
The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction-modification (R-M) enzymes enables temperature-controlled "switching" of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil-globule phase transitions of the polymer as shown in light scattering and gel retardation assays.
Pennadam
Sivanand S
SS
School of Pharmacy and Biomedical Sciences, Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth PO1 2DT, United Kingdom.
Lavigne
Matthieu D
MD
Dutta
Christina F
CF
Firman
Keith
K
Mernagh
Darren
D
Górecki
Dariusz C
DC
Alexander
Cameron
C
D000180:Acrylic Resins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D021122:Protein Subunits
D000180:Acrylic Resins; D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D021122:Protein Subunits; C052970:poly-N-isopropylacrylamide; D004247:DNA; C109829:HsdM protein, Bacteria; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific