Molecular genetic variation in Chinese populations of three subspecies of Hippophae rhamnoides.
To provide a population-level genetic profile for investigation and conservation of genetic diversity of the sea buckthorn (Hippophae rhamnoides L.), 300 individuals of fifteen natural populations of sea buckthorn in China were analyzed by using ISSR (inter-simple sequence repeats) markers. On the basis of Shannon's index and Nei's genetic diversity, the mean genetic diversity detected in the natural populations of Hippophae rhamnoides ssp. yunnanensis, ssp. sinensis, and ssp. gyantsensis was 0.1944, 0.2169, and 0.1372, respectively. The coefficient of gene differentiation (Gst) of seven ssp. yunnanensis populations is 0.2790, and that of seven ssp. sinensis populations is 0.4184. This means that 28% of the total molecular variance of seven ssp. yunnanensis populations existed among populations, and 42% for seven ssp. sinensis populations, suggesting that the subspecies have different genetic structures. No significant correlation between genetic and geographic distances of the populations was found using ISSR markers.
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai, People's Republic of China.
Chunjie
C
Tian
Peng
P
Nan
Suhua
S
Shi
Jiakuan
J
Chen
Yang
Y
Zhong
D002681:China; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D031281:Hippophae / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
D005819:Genetic Markers; D004247:DNA