Fibronectin adsorption on surface-activated poly(dimethylsiloxane) and its effect on cellular function.
This article reports that surface modification of poly(dimethylsiloxane) (PDMS) influences fibronectin (Fn) adsorption and enhances cell attachment. Controlled adsorption of Fn on chemically activated polymer substrates is known to influence cellular function. Thin films of PDMS were spun cast on silicon wafers to obtain homogeneous and molecularly smooth surfaces. The films were made hydrophilic by exposure to ultraviolet ozone activation (PDMS*). The films then were characterized by contact angle goniometry, ellipsometry, atomic force microscopy (AFM), Rutherford backscattering spectrometry and X-ray photoelectron spectroscopy. Contact angle measurements indicated higher hydrophobicity of the nonactivated PDMS substrates than PDMS*. AFM scans of the substrates indicated higher surface roughness of PDMS* (Ra = 0.55 nm) than PDMS (Ra = 0.25 nm). Although Fn surface density (Gamma) was slightly higher on PDMS than on PDMS*, due to hydrophobic interactions between substrate and Fn, cell function was greatly enhanced on the Fn-coated PDMS* (PDMS*-Fn) than on PDMS (PDMS-Fn). Higher attachment of MC3T3-E1 osteoblast-like cells was observed on PDMS*-Fn than on PDMS-Fn. Moreover, cell spreading and cytoskeleton organization after 72 h was clearly favored on the Fn-coated PDMS* surfaces.
Center for Bioactive Materials and Tissue Engineering, Department of Bioengineering, University of Pennsylvania School of Engineering and Applied Science, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, Pennsylvania 19104-6392, USA. gtoworfe@seas.upenn.edu
George K
GK
Toworfe
Russell J
RJ
Composto
Christopher S
CS
Adams
Irving M
IM
Shapiro
Paul
P
Ducheyne
D000199:Actins / Q000378:metabolism; D000327:Adsorption / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D013499:Surface Properties
D000199:Actins; D004129:Dimethylpolysiloxanes; D005353:Fibronectins