Interaction between insulin-like growth factor-1 with other growth factors in serum depleted culture medium for human cartilage engineering.
The regulation roles of insulin-like growth factor-1 (IGF-1) with basic fibroblast growth factor (bFGF) and transforming growth factor beta 2 (TGFbeta2) in human nasal septum chondrocytes monolayer culture and cartilage engineering was investigated in this study. The role of IGF-1 with bFGF and TGFbeta2 was investigated by measuring chondrocyte growth kinetic and collagen genes expression. IGF-1 together with bFGF and TGFbeta2 promote cartilage tissue engineering, increase type II collagen expression and enhance the histological features of engineered cartilage.
Department of Physiology, Medical Faculty of Universiti Kebangsaan Malaysia.
K H
KH
Chua
B S
BS
Aminuddin
N H
NH
Fuzina
B H I
BH
Ruszymah
D002356:Cartilage / Q000637:transplantation*; D002455:Cell Division / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology*; D024043:Collagen Type II / Q000235:genetics; D016895:Culture Media, Serum-Free; D015870:Gene Expression / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D024043:Collagen Type II; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D007334:Insulin-Like Growth Factor I