One third of Danish hypertrophic cardiomyopathy patients with MYH7 mutations have mutations [corrected] in MYH7 rod region.
Familial hypertrophic cardiomyopathy (FHC) is, in most cases, a disease of the sarcomere, caused by a mutation in one of 10 known sarcomere disease genes. More than 266 mutations have been identified since 1989. The FHC disease gene first characterized MYH7, encodes the cardiac beta-myosin heavy chain, and contains more than 115 of these mutations. However, in most studies, only the region encoding the globular head and the hinge region of the mature cardiac beta-myosin heavy chain have been investigated. Furthermore, most studies carries out screening for mutations in the most prevalent disease genes, and discontinues screening when an apparent disease-associated mutation has been identified. The aim of the present study was to screen for mutations in the rod region of the MYH7 gene in all probands of the cohort, regardless of the known genetic status of the proband. Three disease-causing mutations were identified in the rod region in four probands using capillary electrophoresis single-strand conformation polymorphism as a screening method. All mutations were novel: N1327K, R1712W, and E1753K. Two of the probands had already been shown to carry other FHC-associated mutations. In conclusion, we show that in the Danish cohort we find one third of all MYH7 mutations in the rod-encoding region and we find that two of the patients carrying these mutations also carry mutations in other FHC disease genes stressing the need for a complete screening of all known disease genes in FHC-patients.
Department of Clinical Biochemistry, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark.
Lotte
L
Hougs
Ole
O
Havndrup
Henning
H
Bundgaard
Lars
L
Køber
Jens
J
Vuust
Lars Allan
LA
Larsen
Michael
M
Christiansen
Paal Skytt
PS
Andersen
D019943:Amino Acid Substitution / Q000235:genetics*; D024722:Cardiac Myosins; D024741:Cardiomyopathy, Hypertrophic, Familial / Q000235:genetics*; D015331:Cohort Studies; D004252:DNA Mutational Analysis; D003718:Denmark; D005260:Female; D006801:Humans; D008297:Male; D018995:Myosin Heavy Chains / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D024723:Ventricular Myosins / Q000235:genetics*
C501641:MYH7 protein, human; D024722:Cardiac Myosins; D024723:Ventricular Myosins; D018995:Myosin Heavy Chains