Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes.
Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.
Nur Adelina
A N
AN
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Munirah
S
S
Chua
K H
KH
Fuzina
N H
NH
Saim
L
L
Ruszymah
B H I
BH
D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D024042:Collagen Type I / Q000235:genetics*; D024043:Collagen Type II / Q000235:genetics*; D004431:Ear, External; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D024043:Collagen Type II