Effect of recombinant interferon-alpha on streptozotocin-induced chromosome aberrations and sister-chromatid exchanges in hamster cells.
We assessed the effect of recombinant IFN-alpha-2a (rIFN-alpha-2a) on the induction of CAs and sister-chromatid exchanges (SCEs) by the methylating compound streptozotocin (STZ), in Chinese Hamster Ovary (CHO) cells. The cytokine was added to cell cultures 30 min before STZ and left in the culture medium until the end of the treatment. A statistically significant increase in the frequency of CAs and SCEs was observed following treatment with STZ alone (p < 0.05) compared to control, whereas treatments with rIFN-alpha-2a alone did not produce any significant increase of CAs or SCEs over the control values (p < 0.05). Moreover, rIFN-alpha-2a had a marked inhibitory effect on the frequency of STZ-induced CAs--both chromosome- and chromatid-type--(p < 0.05) but was unable to prevent SCEs induced by the antibiotic (p > 0.05). A decrease in the replication index (RI) was observed in the combined treatments compared with STZ alone-treated cultures, indicating inhibition of DNA synthesis. It is suggested that rIFN-alpha-2a exerts its protective action against the induction of CAs by STZ by stimulating DNA repair.
Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900 La Plata, Buenos Aires, Argentina. abolzan@imbice.org.ar
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AD
Bolzán
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E
Lacunza
M S
MS
Bianchi
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols; D002869:Chromosome Aberrations / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D004317:Doxorubicin; D007370:Interferon Type I / Q000494:pharmacology*; D011994:Recombinant Proteins; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013311:Streptozocin / Q000633:toxicity*; D014750:Vincristine
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D007370:Interferon Type I; D011994:Recombinant Proteins; D014750:Vincristine; D013311:Streptozocin; D004317:Doxorubicin; D003520:Cyclophosphamide; D004247:DNA