Differential accumulation of Glut1 in the non-DRM domain of the plasma membrane in response to the inhibition of oxidative phosphorylation.
Approximately 50% of Glut1 in the plasma membrane of Clone 9 cells is localized to the detergent-resistant membrane (DRM) fraction. Acute exposure (90 min) to 5mM azide stimulated glucose transport by approximately 4.7-fold and increased the abundance of Glut1 in the non-DRM fraction of the plasma membrane by approximately 2.9-fold while the abundance of Glut1 in the DRMs was not changed. In parallel experiments, approximately 17 h exposure to azide further increased the rate of glucose transport over that observed at 90 min by approximately 33% and increased plasma membrane Glut1 content by approximately 3.5-fold over control. The increase in total plasma membrane Glut1 reflected a approximately 4.7-fold increase of Glut1 content in the non-DRM fraction and a approximately 2.6-fold increase in the DRMs. We conclude that acute exposure to azide increases Glut1 content in the non-DRM fractions, while prolonged exposure to azide increases the Glut1 content in both non-DRM and DRM fractions. These changes may play an important role in the stimulation of glucose transport in response to the inhibition of oxidative phosphorylation.
Rubin
Darrell
D
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
Ismail-Beigi
Faramarz
F
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002999:Clone Cells; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D007150:Immunohistochemistry; D007700:Kinetics; D008099:Liver / Q000166:cytology; D021962:Membrane Microdomains / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D013347:Subcellular Fractions / Q000737:chemistry / Q000648:ultrastructure
D001386:Azides; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495679:Slc2a1 protein, rat; D005947:Glucose