The chemokine CXCL10 modulates excitatory activity and intracellular calcium signaling in cultured hippocampal neurons.
In this study, we provide evidence for direct modulatory effects of the chemokine, CXCL10, on the physiology of hippocampal neurons maintained in primary culture. CXCL10 elicited a rise in intracellular Ca2+ and enhanced both spontaneous and evoked electrical activity of hippocampal neurons. CXCL10-induced elevations in intracellular Ca2+ were associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and neuronal activity. The effects of CXCL10 were not accompanied by a shift in resting membrane potential (RMP) or input resistance. Expression of the CXCR3 chemokine receptor supports a direct effect of CXCL10 on hippocampal neurons.
Department of Neuropharmacology, CVN-11, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Thomas E
TE
Nelson
Donna L
DL
Gruol
D000200:Action Potentials / Q000276:immunology*; D000818:Animals; D020013:Calcium Signaling / Q000276:immunology*; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D009474:Neurons / Q000276:immunology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000096:biosynthesis
D054357:Chemokine CXCL10; D019743:Chemokines, CXC; C091326:Cxcl10 protein, rat; C515189:Cxcr3 protein, rat; D016207:Cytokines; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine