Determination of CFTR chloride channel activity and pharmacology using radiotracer flux methods.
Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Here, we described three different protocols to study the properties, regulation and pharmacology of the CFTR Cl- channel in populations of cells and artificial vesicles. These techniques are widely used to evaluate the function of wild-type and mutant CFTR prior to detailed analyses using the patch-clamp technique. Moreover, they have proved especially valuable in the search for new drugs to treat cystic fibrosis.
Institut de Physiologie et Biologie Cellulaires CNRS UMR 6187, Université de Poitiers, 40 Avenue du Recteur Pineau, Poitiers, 86022 France.
Caroline
C
Norez
Ghanshyam D
GD
Heda
Timothy
T
Jensen
Ilana
I
Kogan
Lauren K
LK
Hughes
Céline
C
Auzanneau
Renaud
R
Dérand
Laurence
L
Bulteau-Pignoux
Canhui
C
Li
Mohabir
M
Ramjeesingh
Hongyu
H
Li
David N
DN
Sheppard
Christine E
CE
Bear
John R
JR
Riordan
Frédéric
F
Becq
D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects / Q000378:metabolism*; D003584:Cytological Techniques; D006801:Humans; D017136:Ion Transport / Q000502:physiology*; D017736:Ion-Selective Electrodes; D008081:Liposomes / Q000494:pharmacology; D011868:Radioisotopes / Q000494:pharmacology
C505032:CFTR protein, human; D008081:Liposomes; D011868:Radioisotopes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator