Stabilization of phospholipid multilayers at the air-water interface by compression beyond the collapse: a BAM, PM-IRRAS, and molecular dynamics study.
Compression beyond the collapse of phospholipid monolayers on a modified Langmuir trough has revealed the formation of stable multilayers at the air-water interface. Those systems are relevant new models for studying the properties of biological membranes and for understanding the nature of interactions between membranes and peptides or proteins. The collapse of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-di[cis-9-octadecenoyl]-sn-glycero-3-[phospho-l-serine] (DOPS), 1,2-di[cis-9-octadecenoyl]-sn-glycero-3-phosphocholine (DOPC), and 1,2-di[cis-9-octadecenoyl]-sn-glycero-3-[phospho-1-rac-glycerol] (DOPG) monolayers has been investigated by isotherm measurements, Brewster angle microscopy (BAM), and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). In the cases of DMPC and DOPS, the collapse of the monolayers revealed the formation of bilayer and trilayer structures, respectively. The DMPC bilayer stability has been analyzed also by a molecular dynamics study. The collapse of the DOPC and DOPG systems shows a different behavior, and the Brewster angle microscopy reveals the formation of luminous bundles, which can be interpreted as diving multilayers in the subphase.
Saccani
J
J
Laboratoire de Physico-Chimie Moléculaire, UMR 5803-CNRS, 351 cours de la Libération, 33405 Talence Cedex, France.
Castano
S
S
Beaurain
F
F
Laguerre
M
M
Desbat
B
B
D000388:Air; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry*; D008567:Membranes, Artificial; D015394:Molecular Structure; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D011312:Pressure; D012694:Serine / Q000031:analogs & derivatives* / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D008567:Membranes, Artificial; D010743:Phospholipids; D014867:Water; D012694:Serine; D005990:Glycerol; D004134:Dimyristoylphosphatidylcholine