Calorimetric and structural investigation of the interaction of lysozyme and bovine serum albumin with poly(ethylene oxide) and its copolymers.
This work reports investigations aiming at verifying the occurrence of specific interactions between lysozyme or bovine serum albumin (BSA) and poly(ethylene oxide) and its copolymers with poly(propylene oxide). Thermal stability of these proteins, followed by means of high sensitivity DSC, was found to be mostly unaffected by the presence of these polymers. Chromatographic experiments (reverse-phase HPLC and size exclusion chromatrography) did not reveal any sign of specific interaction for these mixtures, either. Isothermal titration calorimetry revealed an increase in enthalpy for the mixtures, represented by a positive enthalpy of transfer for these proteins from buffer to polymer solutions. Moreover, SAXS analyses confirmed that at ambient temperatures these polymers do not affect lysozyme structure. In summary, no evidence is found to support earlier suggestions that some kind of complex could be formed between these proteins and poly(ethylene oxide) or its copolymers, but the present results suggest the occurrence of entropically driven hydrophobic effects.
Almeida
Nara L
NL
Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13083-970 Campinas, SP, Brazil.
Oliveira
Cristiano L P
CL
Torriani
Iris L
IL
Loh
Watson
W
D000818:Animals; D002151:Calorimetry / Q000379:methods; D002417:Cattle; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D009113:Muramidase