Monitoring of the fermentation media of citric acid by the trimethylsilyl derivatives of the organic acids formed.
In this approach, a derivatization method is described for monitoring of organic acids in fermentation media without any separation step. The aqueous phase of fermentation media was evaporated and heated in a silylation reagent to form trimethylsilyl (TMS) derivatives. The silylated compounds are analyzed by 29Si nuclear magnetic resonance (29Si NMR) and gas chromatography-mass spectrometry (GC-MS). 29Si NMR can qualitatively monitor the components produced in the Krebs cycle. Quantification of these compounds is investigated by using selected ion monitoring mode of mass spectrometry. In this mode, mass to charge (m/z) values of their [M - 15]+ ions, which are 465, 275, 247, 221, 335, 251, and 313 of TMS derivatives of citric, alpha-ketoglutaric, succinic, fumaric, l-malic, oxaloacetic, and palmitic (as an internal standard), acids, respectively, are used. The limit of detection and the linear working range for derivatized citric acid were found to be 0.1 mg L(-1) and 10-3 x 10(4) mg L(-1). The relative standard deviation of the method for five replicates was 2.1%. The average recovery efficiency for citric acid added to culture media was approximately 97.2%. Quantitative results of GC-MS are compared with those obtained by an ultraviolet-visible method.
Ghassempour
Alireza
A
Medicinal Plants Research Institute, Shahid Beheshti University, P.O. Box 19835-389, Tehran, Iran. a-ghasempour@cc.sbu.ac.ir
Nojavan
Saeed
S
Talebpour
Zahra
Z
Amiri
Ali Asghar
AA
Najafi
Nahid Mashkouri
NM
D001234:Aspergillus niger / Q000378:metabolism; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry; D019343:Citric Acid / Q000032:analysis* / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D014297:Trimethylsilyl Compounds / Q000032:analysis* / Q000737:chemistry
D002264:Carboxylic Acids; D003470:Culture Media; D014297:Trimethylsilyl Compounds; D019343:Citric Acid