Effects of burn serum on myocardial inflammation and function.
Large cutaneous burns are clearly recognized to produce acute myocardial contractile dysfunction. This study used a model of burn serum challenge in either primary cardiomyocyte cultures or isolated perfused hearts to examine several aspects of burn-serum-related contractile dysfunction as well as myocardial inflammatory responses. Despite the absence of detectable LPS in burn serum, pretreating isolated cells or perfused hearts with recombinant bactericidal permeability-increasing protein (rBPI21) prevented both the inflammatory cytokine cascade and the cardiac contractile dysfunction induced by burn serum treatment of myocytes or ventricular muscle preparations. Our finding that anti-TNF strategies applied to isolated myocytes or hearts before burn serum challenge prevented myocardial inflammation and contractile dysfunction suggested that LPS or LPS-like factors may require the action of second messengers such as TNF-alpha and IL-1beta to mediate LPS-related myocardial depressant effects. Our finding that experimental approaches neutralizing circulating LPS provided cardioprotection suggested that bacterial endotoxin or LPS-like molecules contribute, in part, to burn-related myocardial contractile dysfunction.
Horton
Jureta W
JW
Department of Surgery, UT Southwestern Medical Center, Dallas, TX 75390-9160, USA. jureta.horton@utsouthwestern.edu
Maass
David L
DL
White
D Jean
DJ
Sanders
Billy
B
Murphy
Joseph
J
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002056:Burns / Q000097:blood*; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D007249:Inflammation / Q000276:immunology*; D007375:Interleukin-1 / Q000097:blood / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D032342:Muscle Cells / Q000473:pathology; D009132:Muscles / Q000473:pathology; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000737:chemistry; D012964:Sodium / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C021623:bactericidal permeability increasing protein; D012964:Sodium; D002118:Calcium