Role of allopregnanolone on cerebellar granule cells neurogenesis.
The role of allopregnanolone on immature cerebellar granule cells (CGC) proliferation was studied. Allopregnanolone (0.1-1 microM) increased [(3)H]thymidine incorporation and cell number determined by neuronal counting and by an MTT colorimetric assay. The effect of the neurosteroid was completely prevented by preincubation with 10 mM MgCl(2), 10 microM nifedipine, 10 microM picrotoxin or by 50 microM bicuculine. We conclude that ALLO affects cerebellar neurogenesis by increasing calcium influx through voltage-gated calcium channels and GABA(A) receptors activation.
Instituto de Investigaciones Farmacológicas-CONICET, Buenos Aires, Argentina.
E A
EA
Keller
A
A
Zamparini
L N
LN
Borodinsky
M C
MC
Gravielle
M L
ML
Fiszman
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018118:Chloride Channels / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011280:Pregnanolone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects; D013256:Steroids / Q000494:pharmacology*
D015220:Calcium Channels; D018118:Chloride Channels; D018691:Excitatory Amino Acid Antagonists; D011963:Receptors, GABA-A; D013256:Steroids; D011280:Pregnanolone; D002118:Calcium