Selection of high ginsenoside producing ginseng hairy root lines using targeted metabolic analysis.
To develop an experimental system for studying ginsenoside biosynthesis, we generated thousands of ginseng (Panax ginseng C.A. Meyer) hairy roots, genetically transformed roots induced by Agrobacterium rhizogenes, and analyzed the ginsenosides in the samples. 27 putative ginsenosides were detected in ginseng hairy roots. Quantitative and qualitative variations in the seven major ginsenosides were profiled in 993 ginseng hairy root lines using LC/MS and HPLC-UV. Cluster analysis of metabolic profiling data enabled us to select hairy root lines, which varied significantly in ginsenoside production. We selected hairy root lines producing total ginsenoside contents 4-5 times higher than that of a common hairy root population, as well as lines that varied in the ratio of the protopanaxadiol to protopanaxatriol type ginsenoside. Some of the hairy root lines produce only a single ginsenoside in relatively high amounts. These metabolites represent the end product of gene expression, thus metabolic profiling can give a broad view of the biochemical status or biochemical phenotype of a hairy root line that can be directly linked to gene function.
Woo
Sung-Sick
SS
Unigen Inc. San 80, SongJungLee, ByengCheon, Chungnam 330-863, Korea.
Song
Ji-Sook
JS
Lee
Ju-Yeon
JY
In
Dong Su
DS
Chung
Hwa-Jee
HJ
Liu
Jang Ryol
JR
Choi
Dong-Woog
DW
D036145:Ginsenosides / Q000032:analysis / Q000096:biosynthesis*; D015394:Molecular Structure; D005894:Panax / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012231:Rhizobium
D036145:Ginsenosides