Development of a nonmechanical enucleation method using x-ray irradiation in somatic cell nuclear transfer.
Irradiation of in-vitro-matured bovine oocytes with x-rays of different durations was performed to develop an alternative to conventional mechanical enucleation methods in somatic cell nuclear transfer. No significant difference in embryo development to the blastocyst stage was detected between nonmechanical and mechanical methods, and cytologic analyses of karyotype and microtubule formation showed the potential availability of x-ray irradiation.
Kim
Tae M
TM
School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Hwang
Woo S
WS
Shin
Ji H
JH
Park
Hyun J
HJ
Han
Jae Y
JY
Lim
Jeong M
JM
D000818:Animals; D002417:Cattle / Q000196:embryology*; D002459:Cell Fusion / Q000662:veterinary*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D014965:X-Rays