The alphaM1 segment of the nicotinic acetylcholine receptor exhibits conformational flexibility in a membrane environment.
The transmembrane domain of the nicotinic acetylcholine receptor (nAChR) is predominantly alpha-helical, and of the four distinctly different transmembrane M-segments, only the helicity of M1 is ambiguous. In this study, we have investigated the conformation of a membrane-embedded synthetic M1 segment by solid-state nuclear magnetic resonance (NMR) methods. A 35-residue peptide representing the extended alphaM1 domain 206-240 of the Torpedo californica nAChR was synthesized with specific 13C - and 15N-labelled amino acids, and was incorporated in different phosphatidylcholine model membranes. The chemical shift of the isotopic labels was resolved by magic angle spinning (MAS) NMR and could be related to the secondary structure of the alphaM1 analog at the labelled sites. Our results show that the membrane-embedded alphaM1 segment forms an unstable alpha-helix, particularly near residue Leu18 (alphaLeu223 in the entire nAChR). This non-helical tendency was most pronounced when the peptide was incorporated in fully hydrated phospholipid bilayers, with an estimated 40-50% of the peptides having an extended conformation at position Leu18. We propose that the conserved proline residue at position 16 in the alphaM1 analog imparts a conformational flexibility on the M1 segments that could enable membrane-mediated modulation of nAChR activity.
School of Chemistry, University of Melbourne, Melbourne VIC 3010, Australia. maurits.deplanque@bioch.ox.ac.uk
Maurits R R
MR
de Planque
Dirk T S
DT
Rijkers
Jamie I
JI
Fletcher
Rob M J
RM
Liskamp
Frances
F
Separovic
D000595:Amino Acid Sequence; D000818:Animals; D007554:Isotopes; D008081:Liposomes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D009038:Motion; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines; D018583:Pliability; D011392:Proline; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011978:Receptors, Nicotinic / Q000737:chemistry*; D014101:Torpedo
D007554:Isotopes; D008081:Liposomes; D008565:Membrane Proteins; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D011978:Receptors, Nicotinic; D011392:Proline