High-performance liquid chromatographic analysis of dextromethorphan, guaifenesin and benzoate in a cough syrup for stability testing.
A method has been developed for the analysis of a cough syrup containing dextromethorphan, guaifenesin, benzoic acid, saccharin and other components. Forced degradation was also studied to demonstrate that the method could be employed during a stability study of the syrup. Final conditions were phosphate buffer (25 mM, pH 2.8) with triethylamine (TEA)-acetonitrile (75:25, v/v). In such conditions, all the actives, excipients and degradation products were baseline resolved in less than 14 min, and different wavelengths were used for the different analytes and related compounds.
Sección Química Analítica, Facultad de CC Experimentales y de la Salud, Universidad San Pablo-CEU, Urbanización Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.
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Barbas
D000097:Acetonitriles / Q000737:chemistry; D000996:Antitussive Agents / Q000737:chemistry* / Q000627:therapeutic use; D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003371:Cough / Q000188:drug therapy; D003915:Dextromethorphan / Q000032:analysis*; D004355:Drug Stability; D005021:Ethylamines / Q000737:chemistry; D006140:Guaifenesin / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012439:Saccharin / Q000032:analysis
D000097:Acetonitriles; D000996:Antitussive Agents; D001565:Benzoates; D005021:Ethylamines; D010710:Phosphates; D006140:Guaifenesin; D003915:Dextromethorphan; D012439:Saccharin; C016162:triethylamine; C032159:acetonitrile