Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs.
In previous studies of nuclear transplantation, most cloned animals were obtained by intraspecies nuclear transfer and are phenotypically identical to their nuclear donors; furthermore, there was no further report on successful fish cloning since the report of cloned zebrafish. Here we report the production of seven cross-genus cloned fish by transferring nuclei from transgenic common carp into enucleated eggs of goldfish. Nuclear genomes of the cloned fish were exclusively derived from the nuclear donor species, common carp, whereas the mitochondrial DNA from the donor carp gradually disappeared during the development of nuclear transfer (NT) embryos. The somite development process and somite number of nuclear transplants were consistent with the recipient species, goldfish, rather than the nuclear donor species, common carp. This resulted in a long-lasting effect on the vertebral numbers of the cloned fish, which belonged to the range of goldfish. These demonstrate that fish egg cytoplasm not only can support the development driven by transplanted nuclei from a distantly related species at the genus scale but also can modulate development of the nuclear transplants.
Sun
Yong-Hua
YH
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Chen
Shang-Ping
SP
Wang
Ya-Ping
YP
Hu
Wei
W
Zhu
Zuo-Yan
ZY
D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002347:Carps / Q000196:embryology* / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics / Q000502:physiology*; D019976:Cloning, Organism / Q000379:methods; D003593:Cytoplasm / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D005818:Genetic Engineering; D006054:Goldfish / Q000196:embryology / Q000235:genetics; D006824:Hybridization, Genetic; D053652:Nuclear Transfer Techniques; D038081:Organogenesis / Q000235:genetics / Q000502:physiology*; D010063:Ovum / Q000166:cytology / Q000502:physiology; D010641:Phenotype; D019170:Somites / Q000502:physiology; D013045:Species Specificity; D013131:Spine / Q000196:embryology*
D004272:DNA, Mitochondrial; D004247:DNA