Immunogenicity of murein-associated proteins from temperature-stressed Streptococcus suis cultures.
We compared immunogenicity in pigs of whole cell lysate proteins (WCP) with murein-associated proteins (MAP) obtained from a virulent serotype 2 strain of Streptococcus (S.) suis grown at 32 or 42 degrees C. Protein fractions were tested for their ability to induce antibodies in 3-week-old piglets by enzyme-linked immunosorbent assay and Western blot analysis. We found a significant increase in the antibody levels in all sera irrespective of the preparation used for immunization. However, alpha-WCP sera showed higher reactivities than alpha-MAP sera, and piglets immunized with 32 degrees C preparations (alpha-32 sera) showed higher responses than those immunized with 42 degrees C preparations (alpha-42 sera). Western blot analysis revealed that alpha-WCP sera in part reacted with different proteins when compared with alpha-MAP sera. Furthermore, some proteins were only detected by alpha-32 but not by alpha-42 sera. In conclusion, the results demonstrate the immunogenicity of cell wall MAP of S. suis, and highlight the importance of considering growth conditions in the preparation of subunit vaccines.
Benga
L
L
Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Bischofsholer Damm 15, 30173 Hannover, Germany.
Goethe
R
R
Grosse Beilage
E
E
Valentin-Weigand
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006358:Hot Temperature; D010457:Peptidoglycan / Q000276:immunology*; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification / Q000276:immunology*; D013312:Stress, Physiological; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology*
D000907:Antibodies, Bacterial; D010457:Peptidoglycan