Construct design, biophysical, and biochemical characterization of the fusion core from mouse hepatitis virus (a coronavirus) spike protein.
Membrane fusion between virus and host cells is the key step for enveloped virus entry and is mediated by the viral envelope fusion protein. In murine coronavirus, mouse hepatitis virus (MHV), the spike (S) protein mediates this process. Recently, the formation of anti-parallel 6-helix bundle of the MHV S protein heptad repeat (HR) regions (HR1 and HR2) has been confirmed, implying coronavirus has a class I fusion protein. This bundle is also called fusion core. To facilitate the solution of the crystal structure of this fusion core, we deployed an Escherichia coli in vitro expression system to express the HR1 and HR2 regions linked together by a flexible linker as a single chain (named 2-helix). This 2-helix polypeptide subsequently assembled into a typical 6-helix bundle. This bundle has been analyzed by a series of biophysical and biochemical techniques and confirmed that the design technique can be used for coronavirus as we successfully used for members of paramyxoviruses.
Laboratory of Structural Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Yanhui
Y
Xu
David K
DK
Cole
Zhiyong
Z
Lou
Yiwei
Y
Liu
Lan
L
Qin
Xu
X
Li
Zhihong
Z
Bai
Fang
F
Yuan
Zihe
Z
Rao
George F
GF
Gao
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002942:Circular Dichroism; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D006517:Murine hepatitis virus / Q000737:chemistry* / Q000472:pathogenicity; D017433:Protein Structure, Secondary; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000737:chemistry*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus