Role for a region of helically unstable DNA within the Epstein-Barr virus latent cycle origin of DNA replication oriP in origin function.
The minimal replicator of the Epstein-Barr virus (EBV) latent cycle origin of DNA replication oriP is composed of two binding sites for the Epstein-Barr virus nuclear antigen-1 (EBNA-1) and flanking inverted repeats that bind the telomere repeat binding factor TRF2. Although not required for minimal replicator activity, additional binding sites for EBNA-1 and TRF2 and one or more auxiliary elements located to the right of the EBNA-1/TRF2 sites are required for the efficient replication of oriP plasmids. Another region of oriP that is predicted to be destabilized by DNA supercoiling is shown here to be an important functional component of oriP. The ability of DNA fragments of unrelated sequence and possessing supercoiled-induced DNA duplex destabilized (SIDD) structures, but not fragments characterized by helically stable DNA, to substitute for this component of oriP demonstrates a role for the SIDD region in the initiation of oriP-plasmid DNA replication.
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA.
Zhanna
Z
Polonskaya
Craig J
CJ
Benham
Janet
J
Hearing
D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication; D004278:DNA, Superhelical / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D018741:Replication Origin / Q000502:physiology*; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism; D017735:Virus Latency / Q000502:physiology; D014779:Virus Replication
D004278:DNA, Superhelical; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D035341:Telomeric Repeat Binding Protein 2; D004265:DNA Helicases; C417029:EBV-encoded nuclear antigen 1