High-throughput capillary electrophoresis frontal analysis method for the study of drug interactions with human serum albumin at near-physiological conditions.
The application of the short-end capillary injection to capillary electrophoresis frontal analysis (CE-FA) to study the interaction between basic, neutral and acid drugs towards human serum albumin (HSA) at near-physiological conditions is presented. The compounds selected display a wide range of binding affinities and the results obtained were in good agreement with those reported in the literature. An equation for the estimation of the number of primary binding sites and their corresponding affinity constants is developed isolating the experimentally measured variables in just one axis. The proposed CE-FA method to screen drug interactions with HSA under physiological conditions is simple, rapid and cost-effective what may facilitate its implementation in the drug discovery process.
Departamento Química Analítica, Facultat de Farmacia, Universitat de Valencia, Valencia, Spain.
Juan J
JJ
Martínez-Pla
María A
MA
Martínez-Gómez
Yolanda
Y
Martín-Biosca
Salvador
S
Sagrado
Rosa M
RM
Villanueva-Camañas
Maria J
MJ
Medina-Hernández
D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*
D004364:Pharmaceutical Preparations; D012709:Serum Albumin