F(1)F(0) ATP synthase subunit c is targeted by the SRP to YidC in the E. coli inner membrane.
Escherichia coli inner membrane proteins (IMPs) use different pathways for targeting and membrane integration. We have examined the biogenesis of the F1F0 ATP synthase subunit c, a small double spanning IMP, using complementary in vivo and in vitro approaches. The data suggest that F0c is targeted by the SRP to the membrane, where it inserts at YidC in a Sec-independent mechanism. F0c appears to be the first natural substrate of this novel pathway.
Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
Edwin
E
van Bloois
Gert
G
Jan Haan
Jan-Willem
JW
de Gier
Bauke
B
Oudega
Joen
J
Luirink
D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry*; D021122:Protein Subunits / Q000378:metabolism*; D018271:Signal Recognition Particle / Q000378:metabolism*
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D021122:Protein Subunits; D018271:Signal Recognition Particle; C412443:YIDC protein, E coli; C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases