Genome-wide mutagenesis of Zea mays L. using RescueMu transposons.
Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA. jfernand@stanford.edu
John
J
Fernandes
Qunfeng
Q
Dong
Bret
B
Schneider
Darren J
DJ
Morrow
Guo-Ling
GL
Nan
Volker
V
Brendel
Virginia
V
Walbot
D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D016254:Mutagenesis, Insertional / Q000235:genetics* / Q000379:methods*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D003313:Zea mays / Q000166:cytology / Q000235:genetics*
D004251:DNA Transposable Elements; D018744:DNA, Plant