[Isolation of differentially expressed cDNA sequences in human gastric carcinoma by cDNA microarray].
OBJECTIVE
Some gastric carcinoma-related genes haven't been identified yet. The study was designed to isolate and identify differentially expressed cDNA sequences in gastric carcinoma, and further clone gastric carcinoma-related genes.


METHODS
The differences of gene expression profile between 30 gastric carcinoma tissues and their adjacent normal tissues were analyzed by cDNA microarray which representing about 4 892 cDNA sequences, the differentially expressed cDNA sequences were analyzed by bioinformatics, and selected cDNA sequences were confirmed by reverse transcripase-polymerase chain reaction (RT-PCR).


RESULTS
A total of 33 differentially expressed cDNA sequences were identified in gastric carcinoma, among which 18 up-regulated in gastric carcinoma,while 15 down-regulated. MDSCBC11 clone,represented a novel gene, located in chromosome band 1p35-36, and significantly down-regulated in 13 of 30 (43%) gastric carcinomas.


CONCLUSIONS
Some gastric carcinoma-related cDNA sequences have been identified,they might be involved in pathogenesis of gastric carcinoma. This study provides a useful clue for further clone gastric carcinoma-related genes.
Department of Pathology, Nanjing General Hospital,P.L.A, Nanjing, Jiangsu, 210002, P.R.China.
Hai-Long
HL
Xie
Xiao-Jun
XJ
Zhou
Jie-Yu
JY
Chen
Wen-Bin
WB
Huang
Li-Hua
LH
Zhang
Fang-Qiu
FQ
Li
D000328:Adult; D001483:Base Sequence; D002878:Chromosomes, Human, Pair 1; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger