The fundamentals of tissue engineering: new scaffolds.
The ability to regenerate new bone for skeletal use is a major clinical need. In this study, two novel porous calcium phosphate materials pure HA and biphasic HA/beta-Tricalcium phosphate (HA/beta -TCP) were evaluated as potential scaffolds for cell-seeded bone substitutes using human osteoblast-like cells (HOS) and primary human mesenchymal stem cells (hMSCs). A high rate of proliferation was observed on both scaffolds. A greater increase in alkaline phosphatase (ALP- an indicator of osteoblast differentiation) was observed on HA/beta -TCP compared to HA. This observation indicates that HA/TCP may play a role in inducing osteoblastic differentiation. Although further evaluation is required both materials show potential as innovative synthetic substitutes for tissue engineered scaffolds.
Di Silvio
L
L
Guy's, Kings' and St Thomas' Medical and Dental Institute, King's College London, UK.
Gurav
N
N
Sambrook
R
R
D000818:Animals; D019902:Chondrocytes / Q000166:cytology; D004864:Equipment and Supplies / Q000592:standards; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000592:standards; D007564:Japan; D015603:Keratinocytes / Q000166:cytology; D009924:Organ Culture Techniques / Q000592:standards*; D012449:Safety / Q000592:standards; D023822:Tissue Engineering / Q000592:standards*