Cks1 is dispensable for survival in Saccharomyces cerevisiae.
Cks1 is a small, evolutionarily conserved protein that was identified due to its genetic interaction with the Cdc28 cyclin-dependent kinase. In S. cerevisieae, Cks1 has long been regarded as a protein essential for cell survival. Here, we describe the derivation of viable cks1 null cells. cks1 null cells are slow growing and exhibit a variety of phenotypes consistent with functions previously described for cks1 temperature-sensitive mutants. In addition, we uncovered additional phenotypes (including a meiotic defect, sensitivity to high salt and inositol auxotrophy), all of which are defects associated with mutations in genes involved in general transcription pathways.
Yu
Veronica P C C
VP
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Reed
Steven I
SI
D048868:Adaptor Proteins, Signal Transducing; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D048430:Cell Shape; D005838:Genotype; D006918:Hydroxyurea / Q000494:pharmacology; D007294:Inositol / Q000494:pharmacology; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing; C081185:CKS1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D007294:Inositol; D006918:Hydroxyurea