[Molecular identification of wheat granule bound starch synthase gene polymorphism].
Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE. PCR primers were designed according to the three Wx genes sequences and their mutants, respectively. A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus, respectively, corresponding to the normal and mutant alleles. Compared with the former studies, shorter and more different PCR products at three loci, amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel, which enables screening breeding lines for noodle use faster and effectively.
Song
Jian-Min
JM
Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China.
Li
Bao-Yun
BY
You
Ming-Shan
MS
Liang
Rong-Qi
RQ
Chang
Cheng
C
Liu
Shou-Bin
SB
Tang
Zhao-Hui
ZH
Liu
Guang-Tian
GT
D004591:Electrophoresis, Polyacrylamide Gel; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013214:Starch Synthase / Q000235:genetics*; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D013214:Starch Synthase