Molecular cloning and expression analysis of a macrophage-colony stimulating factor receptor-like gene from rainbow trout, Oncorhynchus mykiss.
The M-CSF and its receptor (M-CSFR, CSF-1R or c-fms proto-oncogene) system were initially implicated as essential in mammals for normal monocyte development as well as for pregnancy. To allow a comparison with the M-CSF and M-CSFR system of an oviparous animal, we cloned a M-CSFR-like gene from rainbow trout (Oncorhynchus mykiss). The gene was cloned from a cDNA library of head kidney. It contained an open reading frame encoding 967 amino acids with a predicted size of 109 kDa. The putative amino acid sequence of rainbow trout M-CSFR showed 54% amino acid identity to fugu (Takifugu rubripes) M-CSFR, 52% to zebrafish (Danio rerio) M-CSFR and 40% to mouse (Mus musculus) and human (Homo sapiens) M-CSFR. The M-CSFR-like gene was constitutively expressed in head kidney, kidney, intestine, spleen and blood. The gene was detected especially in the ovary of immature female rainbow trout. These results suggest that a M-CSFR-like receptor may be involved in female reproductive tracts even in an oviparous animal like fish.
Department of Histology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Teruko
T
Honda
Takashi
T
Nishizawa
Maya
M
Uenobe
Chie
C
Kohchi
Akashi
A
Kuroda
Mitsuru
M
Ototake
Teruyuki
T
Nakanishi
Yuichi
Y
Yokomizo
Yukinori
Y
Takahashi
Hiroyuki
H
Inagawa
Gen-Ichiro
G
Soma
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D016366:Open Reading Frames; D010053:Ovary / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution / Q000235:genetics
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016186:Receptor, Macrophage Colony-Stimulating Factor