Differential expression patterns of proinflammatory and antiinflammatory mediators during atherogenesis in mice.
OBJECTIVE
Recent advances support the current view of atherosclerosis as an inflammatory process that initiates and promotes lesion development to the point of acute thrombotic complications and clinical events. ApoE-deficient mice are a valuable model for studying the involvement of inflammatory mediators during atherogenesis. In this study, we investigated the correlation between atherosclerotic plaque development and expression of important pro- and antiinflammatory mediators during progression of atherosclerosis in ApoE-/- mice.


RESULTS
Expression of proinflammatory cytokines, chemokines, and chemokine receptors within aortic lesions increased during atherogenesis, as detected by real-time quantitative reverse-transcription polymerase chain reaction. In parallel, the number of inflammatory cells within lesions increased together with serum cholesterol and body weight. Interestingly, the majority of inflammatory mediators investigated reached their maximum expression values at 10 weeks of diet, followed by continuous decrease of their expression levels, whereas atherosclerotic plaque size further increased. We show that the expression pattern of these different inflammatory mediators mainly correlates with the amount of inflammatory cells present within the atherosclerotic lesions.


CONCLUSIONS
Atherosclerosis might result from an imbalance between pro- and antiinflammatory mediators in response to endothelial injury induced by cholesterol-rich diet. These data provide important information on the expression kinetics of inflammatory mediators and point out the possible role of antiinflammatory cells during atherogenesis.
Division of Cardiology, Foundation for Medical Research, University Hospital, Geneva, Switzerland.
Niels R
NR
Veillard
Sabine
S
Steffens
Fabienne
F
Burger
Graziano
G
Pelli
François
F
Mach
D000818:Animals; D001012:Aorta, Abdominal / Q000737:chemistry / Q000473:pathology; D001013:Aorta, Thoracic / Q000737:chemistry / Q000473:pathology; D001161:Arteriosclerosis / Q000235:genetics*; D003199:Computer Systems; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics*; D007249:Inflammation / Q000235:genetics; D018836:Inflammation Mediators / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D019707:Receptors, Chemokine / Q000235:genetics; D012850:Sinus of Valsalva / Q000737:chemistry / Q000473:pathology
D018836:Inflammation Mediators; D012333:RNA, Messenger; D019707:Receptors, Chemokine