Encapsulation and/or release behavior of bovine serum albumin within and from polylactide-grafted dextran microspheres.
Polylactide (PLA)-grafted dextran (Dex-graft-PLA) of various contents of sugar units was synthesized by anionic polymerization of L-lactide (L-LA) using the alkoxide of partially trimethylsilylated dextran (TMSDex) and subsequently removing the trimethylsilyl (TMS) groups. The copolymer showed different solubility from L-LA homopolymer with increasing the content of sugar units. We prepared bovine serum albumin (BSA)-loaded microspheres (MS)s according to a water-in-oil-in-water emulsion-solvent evaporation/extraction method using methylene chloride/DMSO as an organic cosolvent. MSs prepared from Dex-graft-PLA [MS(Dex-graft-PLA)s] exhibited higher loading efficiency of BSA than MSs prepared from PLLA [MS(PLLA)s]. The in vitro release rate of BSA from MS(Dex-graft-PLA) was faster than that from MS(PLLA). BSA released from MS(Dex-graft-PLA) maintained the secondary structure of native BSA to a great extent, compared with BSA released from MS(PLLA).
Department of Applied Chemistry, Faculty of Engineering & High Technology Research Center, Kansai University, Suita, Osaka 564-8680, Japan. touchi@ipcku.kansai-u.ac.jp
Tatsuro
T
Ouchi
Toshifumi
T
Saito
Tomohiro
T
Kontani
Yuichi
Y
Ohya
D002214:Capsules; D002942:Circular Dichroism; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D020100:Hydrogels / Q000737:chemistry; D007202:Indicators and Reagents; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D002214:Capsules; D003911:Dextrans; D020100:Hydrogels; D007202:Indicators and Reagents; D011091:Polyesters; C419144:dextran-polylactide hydrogel; D012710:Serum Albumin, Bovine; C033616:poly(lactide); D016650:Fluorescein-5-isothiocyanate