Microaspiration in infants with laryngomalacia.
OBJECTIVE
Laryngomalacia is the most common congenital laryngeal anomaly and the most frequent cause of stridor in infants. Infants with laryngomalacia may have associated respiratory symptoms other than stridor. In this study, we evaluated whether infants with laryngomalacia have episodes of microaspiration by calculating the amount of lipids present in the alveolar macrophages.


METHODS
We reviewed bronchoalveolar lavage (BAL) findings obtained in 14 infants with laryngomalacia and in 11 control children.


METHODS
BAL was performed during flexible fiberoptic bronchoscopy (FFB) to diagnose persistent stridor. The presence of microaspiration was determined by evaluating the amount of lipid per single macrophage, assigning each cell a score from 0 to 4 according to the amount of lipid in the cytoplasm.


RESULTS
The lipid-laden macrophage index was similar in patients and controls (31.5, range 12-177 vs. 45, range 35-106). In 5 of the 14 (36%) patients the index exceeded 100, and more than 25% of the cells were graded 3 and 4, indicating the presence of microaspiration. The lipid-laden macrophage index correlated significantly with the number and percentage of BAL neutrophils (Pearson correlation test, r = 0.83, P < .001 and r = 0.925, P < .001) as a marker of lung inflammation.


CONCLUSIONS
Infants with laryngomalacia may have episodes of microaspiration. BAL performed during FFB to evaluate stridor may add helpful therapeutic information without increasing the risks of the procedure.
Midulla
Fabio
F
Servizio Speciale Fibrosi Cistica, Istituto di Clinica Pediatrica, Università di Roma La Sapienza, Italy. midulla@uniroma1.it
Guidi
Roberto
R
Tancredi
Giancarlo
G
Quattrucci
Serena
S
Ratjen
Felix
F
Bottero
Sergio
S
Vestiti
Katia
K
Francalanci
Paola
P
Cutrera
Renato
R
D001992:Bronchoalveolar Lavage Fluid; D001999:Bronchoscopy; D005260:Female; D006801:Humans; D007223:Infant; D007818:Laryngeal Diseases / Q000150:complications / Q000151:congenital* / Q000175:diagnosis; D007830:Larynx / Q000002:abnormalities*; D008055:Lipids / Q000032:analysis; D016676:Macrophages, Alveolar / Q000737:chemistry; D008297:Male; D011015:Pneumonia, Aspiration / Q000175:diagnosis / Q000209:etiology*; D012016:Reference Values; D012135:Respiratory Sounds / Q000209:etiology*; D013223:Statistics as Topic
D008055:Lipids