HPLC determination and pharmacokinetic studies of salvianolic acid B in rat plasma after oral administration of Radix Salviae Miltiorrhizae extract.
A precise and reproducible HPLC method has been established and validated for determination of salvianolic acid B (SalB) in rat plasma after oral administration of Radix Salviae Miltiorrhizae extract. Liquid-liquid extraction was adopted for the sample preparation. Separation was accomplished on a C(18) column with a linear gradient elution consisting of acetonitrile and aqueous phosphoric acid. Ultraviolet detection was at 280 nm. The method was validated over the concentration range 10.8-259.4 microg/mL using 1 mL of plasma. The assay was linear over this concentration range with a coefficient of variation less than 7%. The extraction recovery of SalB was within the range 71-83% with RSD 11%. The mean recovery of the internal standard was 84% (n = 6) with RSD of 5.6%. This method is suitable to determine SalB in plasma and to investigate the pharmacokinetics of SalB.
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, People's Republic of China.
Jinlan
J
Zhang
Hailan
H
Yu
Yuxin
Y
Sheng
Lie
L
Li
Min
M
Ye
Dean
D
Guo
D000097:Acetonitriles; D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000097:blood / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D008297:Male; D010756:Phosphoric Acids; D010936:Plant Extracts; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza; D012680:Sensitivity and Specificity; D053630:Depsides
D000097:Acetonitriles; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D010756:Phosphoric Acids; D010936:Plant Extracts; C076944:salvianolic acid B; D053630:Depsides; C030242:phosphoric acid; C032159:acetonitrile