Diagnosis of candidosis by amplification of small subunit of 18S rRNA gene.
A PCR assay for the diagnosis of infection produced by Candida sp. was developed. The primers, designated 520 and 522, were selected from highly-conserved areas of the small subunit (ssu) 18S rRNA gene of Candida spp. To check the value of the results a Candida albicans oligonucleotide probe, digoxigenin-labeled, and a general Candida probe were used in hybridization experiments with the amplified products. We were able to detect a Candida- specific fragment of 1800 bp from different clinical samples. The procedure described could provide an interesting complement to present diagnostic methods of detecting Candida sp in clinical samples.
Unzaga
M J
MJ
Servicio de Microbiología, Hospital de Basurto, Avda. Montevideo 18, 48013-Bilbao, Bizkaia, España.
Gallego
L
L
Berdonces
P
P
Dones
J R
JR
Cisterna
R
R