Anti-genotoxic and free-radical scavenging activities of extracts from (Tunisian) Myrtus communis.
The effect of extracts from leaves of Myrtus communis on the SOS reponse induced by Aflatoxin B1 (AFB1) and Nifuroxazide was investigated in a bacterial assay system, i.e. the SOS chromotest with Escherichia coli PQ37. Aqueous extract, the total flavonoids oligomer fraction (TOF), hexane, chloroform, ethyl acetate and methanol extracts and essential oil obtained from M. communis significantly decreased the SOS response induced by AFB1 (10 microg/assay) and Nifuroxazide (20 microg/assay). Ethyl acetate and methanol extracts showed the strongest inhibition of the induction of the SOS response by the indirectly genotoxic AFB1. The methanol and aqueous extracts exhibited the highest level of protection towards the SOS-induced response by the directly genotoxic Nifuroxazide. In addition to anti-genotoxic activity, the aqueous extract, the TOF, and the ethyl acetate and methanol extracts showed an important free-radical scavenging activity towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. These results suggest the future utilization of these extracts as additives in chemoprevention studies.
Laboratoire de Biochimie et de Biologie Moléculaire, Faculté de Pharmacie de Monastir, Rue Avicenne, 5000 Monastir, Tunisie.
N
N
Hayder
A
A
Abdelwahed
S
S
Kilani
R Ben
RB
Ammar
A
A
Mahmoud
K
K
Ghedira
L
L
Chekir-Ghedira
D016604:Aflatoxin B1 / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism*; D006834:Hydrazines / Q000378:metabolism; D062385:Hydroxybenzoates / Q000494:pharmacology; D009152:Mutagenicity Tests; D031651:Myrtus / Q000033:anatomy & histology / Q000737:chemistry*; D009581:Nitrofurans / Q000494:pharmacology; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013014:SOS Response, Genetics
D000890:Anti-Infective Agents; D016587:Antimutagenic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D006834:Hydrazines; D062385:Hydroxybenzoates; D009581:Nitrofurans; D010851:Picrates; D010936:Plant Extracts; D016604:Aflatoxin B1; C004931:1,1-diphenyl-2-picrylhydrazyl; C013150:nifuroxazide