Structure of a major glycolipid from Thermus oshimai NTU-063.
The structure of a major glycolipid isolated from the thermophilic bacteria Thermus oshimai NTU-063 was elucidated. The sugar and fatty acid compositions were determined by GC-MS and HPLC analysis on their methanolysis and methylation derivatives, respectively. After removal of both O- and N-acyl groups by alkaline treatment, the glycolipid was converted to a fully acetylated tetraglycosyl glycerol derivative, the structure of which was then determined by NMR spectroscopy (TOCSY, HSQC, HMBC). Thus, the complete structure of the major glycolipid from T. oshimai NTU-063 was established as beta-Glcp-(1-->6)-beta-Glcp-(1-->6)-beta-GlcpNAcyl-(1-->2)-alpha-Glcp-(1-->1)-glycerol diester. The N-acyl groups on the 2-amino-2-deoxy-glucopyranose residue are C15:0 and C17:0 fatty acids, whereas the fatty acids of glycerol diester are more heterogeneous including both straight and branched fatty acids from C15:0 to C18:0.
Lu
Tzu-Li
TL
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
Chen
Chien-Sheng
CS
Yang
Feng-Ling
FL
Fung
Jim-Min
JM
Chen
Mao-Yen
MY
Tsay
San-San
SS
Li
Jianjun
J
Zou
Wei
W
Wu
Shih-Hsiung
SH
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D013824:Thermus / Q000737:chemistry*
D002241:Carbohydrates; D005227:Fatty Acids; D006017:Glycolipids