Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils.
A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C18 column. Methanol and acetonitrile (1:1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL.
Faculty of Commodity Science, Poznań University of Economics, al Niepodległości 10, 60-967 Poznań, Poland. a.gliszczynska-swiglo@ae.poznan.pl
Anna
A
Gliszczyńska-Swigło
Ewa
E
Sikorska
D019840:2-Propanol / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D024505:Tocopherols / Q000032:analysis*
D000097:Acetonitriles; D010938:Plant Oils; D019840:2-Propanol; D024505:Tocopherols; D000432:Methanol; C032159:acetonitrile