Measurements of airway surface liquid height and mucus transport by fluorescence microscopy, and of ion composition by X-ray microanalysis.
The respiratory tract is lined by a thin layer of fluid, the airway surface liquid (ASL), which plays a critical role in lung defense. The paper describes methods to determine the height of the ASL and corresponding mucus transport rates using fluorescent probes, and methods to determine the ionic composition of the ASL by X-ray microanalysis.
Roomans
Godfried M
GM
Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75123 Uppsala, Sweden. godfried.roomans@medcellbiol.uu.se
Kozlova
Inna
I
Nilsson
Harriet
H
Vanthanouvong
Viengphet
V
Button
Brian
B
Tarran
Robert
R
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001826:Body Fluids / Q000737:chemistry / Q000502:physiology*; D018929:Cell Culture Techniques; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D009093:Mucus / Q000502:physiology*; D020545:Respiratory Mucosa / Q000502:physiology*; D013500:Surface Tension
D005456:Fluorescent Dyes