Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans.
A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.
Yang
Lin
L
Department of Molecular Genetics, The Ohio State University, 804 Riffe Building, 496 West 12th Ave., Columbus, OH 43210, USA.
Ukil
Leena
L
Osmani
Aysha
A
Nahm
Francis
F
Davies
Jonathan
J
De Souza
Colin P C
CP
Dou
Xiaowei
X
Perez-Balaguer
Ariadna
A
Osmani
Stephen A
SA
D001233:Aspergillus nidulans / Q000166:cytology / Q000235:genetics*; D002453:Cell Cycle; D002503:Centromere / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics
D005656:Fungal Proteins; D005819:Genetic Markers; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins