Horseradish peroxidase-catalyzed cross-linking of feruloylated arabinoxylans with beta-casein.
Heterologous conjugates of wheat arabinoxylan and beta-casein were prepared via enzymatic cross-linking, using sequential addition of the arabinoxylan to a mixture of beta-casein, peroxidase, and hydrogen peroxide. The maximal formation of adducts between the beta-casein and the feruloylated arabinoxylan was reached at a protein-to-arabinoxylan ratio of 10:1, in combination with a molar ratio hydrogen peroxide to substrate of 2:1 and a molar protein-to-enzyme ratio between 10(2) and 10(4). The protein-arabinoxylan adducts were separated from the arabinoxylan homopolymers by size exclusion and anion exchange chromatography. The molar ratio protein:arabinoxylan in the purified conjugates varied between 0.1 and 5.6. This is the first report on the large-scale enzymatic preparation of heterologous protein-arabinoxylan conjugates.
Agrotechnology & Food Innovations, Bornsesteeg 59, 6708 PD Wageningen, The Netherlands. Carmen.Boeriu@wur.nl
Carmen G
CG
Boeriu
Gideon
G
Oudgenoeg
Willem Th J
WT
Spekking
Lucienne B J M
LB
Berendsen
Laetitia
L
Vancon
Hans
H
Boumans
Harry
H
Gruppen
Willem J H
WJ
van Berkel
Colja
C
Laane
Alphons G J
AG
Voragen
D002364:Caseins / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D003432:Cross-Linking Reagents; D006735:Horseradish Peroxidase / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014908:Triticum / Q000737:chemistry; D014990:Xylans / Q000378:metabolism*
D002364:Caseins; D003373:Coumaric Acids; D003432:Cross-Linking Reagents; D014990:Xylans; C085118:arabinoxylan; C004999:ferulic acid; D006735:Horseradish Peroxidase