Overexpression of genes in Purkinje cells in the embryonic chicken cerebellum by in vivo electroporation.
In cerebellar development, Purkinje cells transiently express various genes, whose expression is compartmentalized into different mediolaterial or parasagittal domains. The study of these genes will help us to understand the functional organization of the cerebellum. In the present work, we establish an efficient in vivo electroporation technique to transfer genes into Purkinje cells of the developing chicken cerebellum, as demonstrated by green fluorescent protein. This method can be applied to analyze gene function and regulation in cerebellar Purkinje cells.
Luo
Jiankai
J
Institute of Anatomy I, Friedrich Schiller University, Teichgraben 7, D-07743 Jena, Germany. tluo@mt.uni-jena.de
Redies
Christoph
C
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D002642:Chick Embryo; D018274:Electroporation / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*