Conformational transitions and glycation of serum albumin in patients with minimal-change glomerulopathy.
BACKGROUND
There has been a lack of study on the structural changes of serum albumin in patients with minimal change disease (MCD). To determine whether glycation and/or conformational transitions of albumin are involved in the pathogenesis of albuminuria, nine patients with MCD were enrolled in a prospective follow-up study for comparison of these parameters in serum albumin during the remission and relapse of nephrotic syndrome.


METHODS
Circular dichroism measurements were made with purified albumin. Ellipticities at each wavelength were transformed to mean residue ellipticity. Monosaccharide composition was analyzed by high-pH anion-exchange chromatography with pulsed amperometric detection.


RESULTS
There was no difference in the proportions of alpha-helix, beta-conformation, and beta-turn of albumin between the sera of control patients and those with nephrotic syndrome. However, the proportion of the random configuration was slightly higher in the plasma albumin of patients in relapse than in those in remission. The proportion of the random configuration was lower in the albumin of the serum than in the urine of patients with nephrotic syndrome, but there was no difference in the proportions of alpha-helix, beta-conformation, and beta-tum of albumin between their plasma and urine.


CONCLUSIONS
Our results suggest that conformational changes in albumin are involved in albuminuria in patients with MCD.
Hong
Sae Yong
SY
Department of Internal Medicine and Clinical Research Institute, Soonchunhyang University Chonan Hospital, Chonan, Korea.
Lee
Eun Young
EY
Yang
Jong Oh
JO
Kim
Tae Yeong
TY
Kim
Eun Hee
EH
Cheong
Mi Young
MY
Kim
Soo Hyun
SH
Cheong
Chae Joon
CJ
D000328:Adult; D000419:Albuminuria / Q000652:urine; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009402:Nephrosis, Lipoid / Q000097:blood* / Q000652:urine; D009404:Nephrotic Syndrome / Q000097:blood / Q000652:urine; D011446:Prospective Studies; D012709:Serum Albumin / Q000737:chemistry*
D012709:Serum Albumin