[High mobility group box-1 protein activates Janus kinase-signal transducer and activator of transcription pathway in rat peritoneal macrophages].
OBJECTIVE
To investigate the potential signal transduction mechanism in high mobility group box-1 protein (HMGB1)-induced inflammatory response in rat peritoneal macrophages.


METHODS
Peritoneal macrophages obtained from male Wistar rats were incubated for 3 days before they were stimulated by HMGB1 (10 microg/ml). At various time points after HMGB1 stimulation, macrophages were denatured directly in cell culture flasks to detect activation of Janus kinase-2 (JAK2), signal transducer and activator of transcription-1 (STAT1) and STAT3 by immunoprecipitation, Western blotting and electrophoretic mobility shift assay, respectively.


RESULTS
HMGB1 stimulation could activate STAT1 and STAT3 in peritoneal macrophages in 2 hours, among them the activation of STAT3 appeared to be the quickest, peaking as early as 10 minutes after stimulation. But no marked change in JAK2 activity was observed within 2 hours following HMGB1 stimulation.


CONCLUSIONS
These data suggest that Janus kinase-signal transducer and activator of transcription pathway might be involved in regulation of HMGB1-induced inflammatory response in peritoneal macrophages.
Burns Institute, 304th Hospital of PLA, Beijing 100037, China.
Hui
H
Liu
Yong-Ming
YM
Yao
Yue-Qing
YQ
Dong
Yan
Y
Yu
Zhi-Yong
ZY
Sheng
D000818:Animals; D002478:Cells, Cultured; D024243:HMGB1 Protein / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D050794:STAT1 Transcription Factor / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction
D024243:HMGB1 Protein; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; C494081:Stat1 protein, rat; C494088:Stat3 protein, rat; C507927:Jak2 protein, rat; D053614:Janus Kinase 2