Detection rates of bacteria in chronic otitis media with effusion in children.
This study was performed to investigate polymerase chain reaction-based detection of bacterial DNA in middle ear fluid and assess the correlation between the PCR-positive rate with several factors associated with middle ear effusion. The purpose was to gain a further understanding of bacterial infection as a major cause of otitis media with effusion. Of the 278 specimens of middle ear fluid, 39 (14%) tested positive by ordinary culture. The overall detection rate of bacterial DNA using the PCR method was 36.7% for middle ear effusion, and bacterial DNA detection rates of Hemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis in the middle ear effusion were 29.1%, 4.7% and 10.8%, respectively. The bacterial DNA detection rate was higher in ears with a history of acute otitis media than those without the history. High detection rates were observed in patients younger than 48 months who have had a higher tendency to present with acute otitis media. We concluded that PCR is a more sensitive method for the detection of bacteria in middle ear effusion than ordinary culture, and acute otitis media is a major contributor to the pathogenesis of otitis media with effusion.
Department of Otolaryngology, College of Medicine, Hanyang University, Seoul, Korea.
Chul-Won
CW
Park
Jang-Hee
JH
Han
Jin-Hyeok
JH
Jeong
Seok-Hyun
SH
Cho
Mi-Jung
MJ
Kang
Kyung
K
Tae
Seung-Hwan
SH
Lee
D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D006192:Haemophilus Infections / Q000175:diagnosis*; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D001936:Moraxella catarrhalis / Q000235:genetics / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000175:diagnosis; D010034:Otitis Media with Effusion / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000175:diagnosis; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial