Immunogenicity of recombinant LT-B delivered orally to humans in transgenic corn.
Previous clinical studies have demonstrated the feasibility of using edible transgenic plants to deliver protective antigens as new oral vaccines. Transgenic corn is particularly attractive for this purpose since the recombinant antigen is stable and homogeneous, and corn can be formulated in several edible forms without destroying the cloned antigen. Transgenic corn expressing 1 mg of LT-B of Escherichia coli without buffer was fed to adult volunteers in three doses, each consisting of 2.1 g of plant material. Seven (78%) of nine volunteers developed rises in both serum IgG anti-LT and numbers of specific antibody secreting cells after vaccination. Four (44%) of nine volunteers also developed stool IgA. Transgenic plants represent a new vector for oral vaccine antigens.
Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore Street, Baltimore, MD 21201, USA. ctacket@medicine.umaryland.edu
Carol O
CO
Tacket
Marcela F
MF
Pasetti
Robert
R
Edelman
John A
JA
Howard
Stephen
S
Streatfield
D000284:Administration, Oral; D000328:Adult; D000917:Antibody Formation / Q000276:immunology; D000921:Antibody-Producing Cells / Q000378:metabolism; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000276:immunology*; D004768:Enterotoxins / Q000008:administration & dosage / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000276:immunology*; D005243:Feces / Q000737:chemistry; D005433:Flour / Q000032:analysis; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C027538:heat-labile enterotoxin, E coli