Generation of multinucleated giant cells in vitro from bovine monocytes and macrophages.
The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNgamma in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), MGC formations from macrophages were observed only when macrophages were cultured with GM-CSF plus CM. These results indicate that several mechanisms to generate MGC from bovine monocytes-macrophage lineage cells exist, and that GM-CSF is a major mediator of MGC formation in cattle.
National Institute of Animal Health, Tsukuba, Japan.
Kazuhiro
K
Yoshihara
Reiko
R
Nagata
Yoshihiro
Y
Muneta
Shigeki
S
Inumaru
Yuichi
Y
Yokomizo
Yasuyuki
Y
Mori
D000818:Animals; D002417:Cattle / Q000276:immunology*; D003208:Concanavalin A; D005453:Fluorescence; D015726:Giant Cells / Q000166:cytology / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D066298:In Vitro Techniques; D008264:Macrophages / Q000502:physiology*; D008863:Microspheres; D009000:Monocytes / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology
D003208:Concanavalin A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor