RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts.
Staphylococcus aureus is one of the most important pathogens in humans and animals. In this study eighty strains were analyzed by RAPD-PCR to assess the genetic relationship between S. aureus isolates from bovine and human hosts. Results were compared with those obtained by biotyping. Fifty-two percent of the S. aureus isolates belonged to a host specific biotype (human, bovine and poultry). Bovine and human ecovars were the most prevalent. Dendrogram obtained by RAPD results showed that all the isolates clustered into eleven groups (A-K) at a relative genetic similarity of less than 30% when analyzed with the three primers. Group A clustered 95% of the human host isolates and the remaining groups (B-K) clustered the bovine host isolates. Principal coordinate analysis also showed that the isolates could be arbitrarily divided into two groups, bovine and human, by the second coordinate. Only 9 isolates (11%) were not clustered into these groups. The genetic diversity among the S. aureus isolates from bovine hosts is relatively low compared to that of isolates from human hosts. There were no statistically significant differences among isolated from bovine and human hosts. This study shows that RAPD-PCR assayed with three primers can be successfully applied to assess the genetic relationship of S. aureus isolates from different hosts.
Reinoso
Elina
E
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Qúimicas y Naturales, Universidad Nacional de Río Cuarto, C.R 5800 Ruta 36 km 601. Río Cuarto, Córdoba, Argentina.
Bettera
Susana
S
Frigerio
Cecilia
C
DiRenzo
Miguel
M
Calzolari
Aldo
A
Bogni
Cristina
C
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003612:Dairying; D005260:Female; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology*; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification