Establishment of in vitro test system for the evaluation of the estrogenic activities of natural products.
In order to evaluate estrogenic compounds in natural products, an in vitro detection system was established. For this system, the human breast cancer cell line MCF7 was stably transfected using an estrogen responsive chloramphenicol acetyltransferase (CAT) reporter plasmid yielding MCF7/pDsCAT-ERE119-Ad2MLP cells. To test the estrogenic responsiveness of this in vitro assay system, MCF7/pDsCAT-ERE119-Ad2MLP cells were treated with various concentrations of 17beta-estradiol. Treatments of 10(-8) to 10(-12) M 17beta-estradiol revealed significant concentration dependent estrogenic activities compared with ethanol. We used in vitro assay system to detect estrogenic effects in Puerariae radix and Ginseng radix Rubra extracts. Treatment of 500 and 50 microg/ml of Puerariae radix extracts increased the transcriptional activity approximately 4- and 1.5-fold, respectively, compared with the ethanol treatment. Treatment of 500, 50, and 5 microg/ml of Ginseng radix Rubra extracts increased the transcriptional activity approximately 3.2-, 2.7-, and 1.4-fold, respectively, compared with the ethanol treatment. These observations suggest that Puerariae radix and Ginseng radix Rubra extracts have effective estrogenic actions and that they could be developed as estrogenic supplements.
Department of Bioscience and Biotechnology, Silla University, Busan 617-736, Korea.
Ok Soo
OS
Kim
Jung-Hye
JH
Choi
Young Hwa
YH
Soung
Seon Hee
SH
Lee
Jae-Hwa
JH
Lee
Jong Myung
JM
Ha
Bae-Jin
BJ
Ha
Moon-Soo
MS
Heo
Sang-Hyeon
SH
Lee
D000818:Animals; D001688:Biological Products / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D005894:Panax; D010936:Plant Extracts / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D029908:Pueraria; D014981:Xenopus
D001688:Biological Products; D004967:Estrogens; D010936:Plant Extracts; D004958:Estradiol