Evaluation of the Salmonella umu test with 83 NTP chemicals.
There is a need for simple rapid tests for evaluating the carcinogenic potential of the thousands of chemical compounds that are developed each year. The DNA-damaging effects of 83 National Toxicology Program (NTP) chemicals, including noncarcinogens and carcinogens, were examined in the umu test by measuring the expression of the umuDC-lacZ genes in Salmonella typhimurium TA1535/pSK1002. Salmonella were exposed to individual NTP chemicals at 37 degrees C for 2 hr both with and without a rat liver S9 mix; the treated cells were then diluted and incubated for a further 2 hr (posttreatment assay). O-nitrophenyl-beta-D-galactoside was added to the cultures and the beta-galactosidase activity driven by the Salmonella umuDC-lacZ genes was determined by measurement of the OD(420 nm) and OD(550 nm) of the cultures. Salmonella cell number was simultaneously determined by measurement of OD(600 nm). The overall concordance between genotoxicity in the umu test and carcinogenicity was 67%, which was similar to the concordance between Ames' test results and carcinogenicity (63%) using the same 83 NTP chemicals. The results of this study indicate that the umu test with a single Salmonella strain is a simple rapid system, with accuracy comparable to existing, more time-consuming assays.
Genetic Toxicology Group, Toxicology Division II, Mitsubishi Chemical Safety Institute Ltd., Ibaraki 314-0255, Japan. k-yasunaga@ankaken.co.jp
Katsuaki
K
Yasunaga
Akiko
A
Kiyonari
Takeshi
T
Oikawa
Naoki
N
Abe
Kunie
K
Yoshikawa
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods; D002273:Carcinogens / Q000633:toxicity*; D016208:Databases, Factual; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial / Q000187:drug effects*; D007287:Inorganic Chemicals / Q000633:toxicity*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods; D009930:Organic Chemicals / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013014:SOS Response, Genetics / Q000187:drug effects / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D002273:Carcinogens; D007287:Inorganic Chemicals; D009930:Organic Chemicals; D001616:beta-Galactosidase