Sequential monolayer-suspension culture of human airway epithelial cells.
In this article, we describe a technique for culturing human airway epithelial cells, developed in Leuven, as a new tool for a most reliable diagnosis for ciliary disorders. This technique that allows to keep both structural and functional primary abnormalities of inherited ciliary abnormalities, while avoiding the secondary ones, can also be useful to a number of other studies, namely in cystic fibrosis.
Willems
Tom
T
Experimental Otorhinolaryngology, Department for Human Genetics, Campus GHB, O&N nivo 6, Herestraat 49, B-3000 Leuven, Belgium.
Jorissen
Mark
M
D018929:Cell Culture Techniques; D004847:Epithelial Cells; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D020545:Respiratory Mucosa / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000379:methods