Isolation and characterization of alpha-glucosidase inhibitor from the fungus Ganoderma lucidum.
An alpha-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of alpha-glucosidase. It showed very potent inhibitory activity against alpha-glucosidase with an IC50 value of 4.6 micro g/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of alpha-glucosidase was competitive.
Kim
Shin-Duk
SD
Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea. sdkim@skuniv.ac.kr
Nho
Hong Joon
HJ
D001667:Binding, Competitive; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D020128:Inhibitory Concentration 50; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry / Q000254:growth & development*; D000520:alpha-Glucosidases / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000520:alpha-Glucosidases