Activity of some hepatic enzymes in schistosomiasis and concomitant alteration of arylsulfatase B.
The levels of arylsulfatases A and B, alpha-amylase, aspartate transcarbamylase, and gamma-glutamyl transpeptidase were investigated during the infection of mice with schistosoma mansoni. This infection caused a significant (p < 0.001) increase in the activity of hepatic arylsulfatase B (ASB), aspartate transcarbamylases and gamma-glutamyl transpeptidase. A non-significant difference occurred for alpha-amylase (p < 0.3) and arylsulfatase A (p > 0.5) when compared to the control. The specific activity of hepatic ASB was progressively increased with the progression of the Schistosoma-infection. Moreover, the kinetic studies of hepatic ASB in Schistosoma-infection showed that a slight decrease in the value of K(m) and about a 40% increase in V(max) when compared to the control. In addition, the pH optimum of hepatic ASB was altered from 6 to 7 as a result of schistosomiasis. These observations suggest that there are schistosomiasis-associated changes of the catalytic and kinetic properties of hepatic ASB.
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt. m_balbaa@hotmail.com
Mahmoud
M
Balbaa
Mohamed
M
El-Kersh
Hamdy
H
Mansour
Galila
G
Yacout
Mohamed
M
Ismail
Ahmed
A
Malky
Khaled
K
Bassiouny
Nihad
N
Abdel-Monem
Kamal
K
Kandeel
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000378:metabolism*; D002384:Catalysis; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008109:Liver Diseases, Parasitic / Q000201:enzymology; D008297:Male; D051379:Mice; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000469:parasitology; D012552:Schistosomiasis / Q000201:enzymology*; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D001221:Aspartate Carbamoyltransferase; D005723:gamma-Glutamyltransferase; D020051:N-Acetylgalactosamine-4-Sulfatase; D002553:Cerebroside-Sulfatase; D000516:alpha-Amylases