An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements.
An attempt was made to develop an interclass somatic cell nuclear transfer method as an alternative means of establishing chicken embryonic stem cells. Chicken-to-cattle interclass embryos that activated calcium ionophore, cycloheximide, and cytochalasin D were developed into blastocysts, and the developing interclass embryos had chicken genetic complements.
Kim
Tae M
TM
Division of Animal Genetic Engineering, School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Park
Tae S
TS
Shin
Sang S
SS
Han
Jae Y
JY
Moon
Shin Y
SY
Lim
Jeong M
JM
D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000235:genetics; D002459:Cell Fusion / Q000379:methods*; D002467:Cell Nucleus / Q000235:genetics; D002642:Chick Embryo / Q000196:embryology*; D005260:Female; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology