Cyclin D1, p53, MIB1, intratumoral microvessel density, and human papillomavirus in advanced laryngeal carcinoma: association with nodal metastasis.
OBJECTIVE
We sought to study various parameters in laryngeal squamous cell carcinoma (LSCC) that might predict nodal metastasis.


METHODS
Sixty-four LSCCs were examined with respect to their histopathology and, using immunohistochemistry, their proliferative capacity (MIB1), p53 and cyclin D1 status, and intratumoral microvessel density. The presence of human papillomavirus was ascertained by the polymerase chain reaction.


RESULTS
Histopathologically, most tumors had an infiltrating/mixed growth pattern and a diminished inflammatory reaction at the growing margin. In addition, 56% of the tumors were positive for MIB1, with 64% showing p53 overexpression; 70% were positive for cyclin D1; and 59% showed increased tumor microvessel density. Of 42 cases analyzed, 9.5% were positive for human papillomavirus 16.


CONCLUSIONS
Of the parameters studied, a diminished lymphocytic inflammatory response at the periphery (P < 0.05) and cyclin D1 overexpression (P < 0.001) correlated significantly with cervical nodal metastasis at presentation.


CONCLUSIONS
Cyclin D1 overexpression, easily assessed on biopsy samples, may thus help in optimizing therapy at the outset.
Kumar
Rekha V
RV
Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore, India. kvijaykumar@vsnl.com
Shenoy
Ashok M
AM
Daniel
Richard
R
Shah
Keerti V
KV
D000293:Adolescent; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000473:pathology* / Q000821:virology; D019938:Cyclin D1 / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000098:blood supply / Q000737:chemistry / Q000473:pathology* / Q000821:virology; D008207:Lymphatic Metastasis / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; C077171:HIVEP2 protein, human