Effect of lead ions on rat erythrocyte purine content.
The influence of short-term exposure to lead on the energetic status of erythrocytes in rats is reported in this study. The male Wistar rats selected for this study drank water containing 1% lead(II) acetate and/or intraperitoneal injections of 1 or 2 mg/kg body wt every 4 d starting on the eighth of the experiment, over a period of 1 mo. The whole-blood lead concentration measured after 4 wk was 1.51-35.31 microg/dL. The concentrations of adenosine, adenosine triphosphates, diphosphates, and monophosphates (ATP, ADP, and AMP), guanine triphosphates, diphosphates and monophosphates (GTP, GDP, and GMP), guanosine (Guo), inosine (Ino), inosine monophosphate (IMP), hypoxantine (Hyp), and nicotinamide dinucleotide and its phosphate (NAD(+) and NADP(+)) were determined by high-performance liquid chromatography (HPLC). The mean concentrations of ATP, GTP, NAD(+), and NADP(+) and those of adenylate (AEC) and guanylate (GEC) were significantly reduced in erythrocytes from the animals exposed to lead when compared to untreated controls. These results suggest that a lead ion disrupts the erythrocyte energy pathways. The decreases of NAD(+) and ATP could be used as an indicator of the extent of exposure to low levels of lead.
Department of Biochemistry, Faculty of Natural Sciences, University of Szczecin, Szczecin, Poland.
Irena
I
Baranowska-Bosiacka
Alina Joanna
AJ
Hlynczak
D000227:Adenine Nucleotides / Q000097:blood; D000284:Administration, Oral; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D006150:Guanine Nucleotides / Q000097:blood; D019271:Hypoxanthine / Q000097:blood; D007274:Injections, Intraperitoneal; D007292:Inosine Nucleotides / Q000097:blood; D008297:Male; D009243:NAD / Q000097:blood; D009249:NADP / Q000097:blood; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D011685:Purine Nucleotides / Q000097:blood*; D011687:Purines / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D009942:Organometallic Compounds; D011685:Purine Nucleotides; D011687:Purines; D009243:NAD; D019271:Hypoxanthine; D009249:NADP; C008261:lead acetate