[Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene].
OBJECTIVE
To carry out bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene (tlh) obtained by PCR amplification.


METHODS
The tlh gene amplified by PCR was cloned into the vector pET32a(+) and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.


CONCLUSIONS
The sequenced tlh gene (named tlh14-90) was entered into GenBank with the accession number of AY289609. Tlh14-90 has a length of 1 257 bp with both start and stop codons, having 99% homology with the tlh gene of WP1. Tlh14-90 is predicted to encode a protein containing 418 amino acids (named TLH14-90, with the molecular formula of C(2131)H(3184)N(548)O(649)S(16), molecular weight of 47 392.9, and the theoretical PI of 4.92). This protein consists of 4 Cys and the contents of Ala, Leu and Asn are 11.0%, 7.4% and 7.2%, respectively, having a hydrophobic parameter of -34 calculated using kappa-D method. Predicted as a alpha/protein for its secondary structure, TLH14-90 has not been identified for its tertiary structure.
Department of Epidemiology, Southern Medical University, Guangzhou 510515, China. sdlizf@163.com
Zhi-feng
ZF
Li
Jun
J
Nie
Ying-chun
YC
Dai
Jian-dong
JD
Li
Qing
Q
Chen
Shou-yi
SY
Yu
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014736:Vibrio parahaemolyticus / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio