Induced angiogenesis with intramedullary direct current: experimental research.
The purpose of this study was to evaluate angiogenesis after the use of intramedullary direct electrical current in rabbit tibia. Thirty-two New Zealand rabbits were divided into four groups: group 1, false electrode group; group 2, hole group; group 3, control group; and group 4, intramedullary electrical stimulation group. One-half of the rabbits in each group were evaluated angiographically, pathologically, and scintigraphically on day 7, and the rest were evaluated on day 21. Results proved that electrical stimulation was not capable of the induction of angiogenesis in the subjects killed on day 7 and day 21. Furthermore, we found some fibrotic changes secondary to electrical stimulation on day 7 (P = 0.04) and day 21 (P = 0.01). However, an increase in new capillary vessels occurred in the false electrode group (P = 0.02). We found no useful effect of electrical stimulation in our study, a finding that is possibly due to our use of a method previously undocumented in the literature. We believe that this study can be the new baseline for further studies into the stimulation or inhibition of angiogenesis using intramedullary wire with or without electrical stimulation.
Department of Orthopedics and Traumatology, Inönü University and Turgut Ozal Medical Center, Malatya, Turkey. minan@inonu.edu.tr
Muharrem
M
Inan
Ilker
I
Alat
Iclal
I
Gurses
Ersoy
E
Kekilli
Ramazan
R
Kutlu
Ahmet
A
Eskin
Omer Murat
OM
Aydin
Haci
H
Bostan
Saim
S
Yologlu
D000792:Angiography; D000818:Animals; D002196:Capillaries / Q000502:physiology; D004599:Electric Stimulation Therapy / Q000009:adverse effects / Q000379:methods*; D005355:Fibrosis; D007511:Ischemia / Q000628:therapy*; D018482:Muscle, Skeletal / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011817:Rabbits; D013977:Tibia / Q000098:blood supply* / Q000473:pathology