[A study of quadriplex PCR with chimeric primers for short tandem repeats loci].
OBJECTIVE
To establish a simplified method of multiplex PCR based on chimeric primers for STR loci.


METHODS
A set of chimeric primers and universal primers were designed to carry out a multiplex PCR for amplifying four short tandem repeats(STR) loci. Then the amplified STR loci were detected by polyacrylamide gel electrophoresis and the gels were sliverstained.


RESULTS
A quadriplex STR system was developed on the basis of both chimeric and universal primers.


CONCLUSIONS
The STR multiplex PCR based on both chimeric and universal primers hss been achieved readily and reproducibly by simple adjustment of the individual primer concentrations. The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing the multiplex PCR.
Dong
Jian-guo
JG
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P.R. China. rechtsme@weums.edu.cn
Ji
Qiang
Q
Li
Ying-bi
YB
Wu
Jin
J
Zhang
Hai-jun
HJ
Hou
Yi-ping
YP
D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers