Identification of a 35 kDa protein in rat spinal ganglia and sensory fibers.
A 35 kDa protein was purified from rat spinal ganglia and sensory fibers. Combined direct trypsin digest and liquid chromatography ion trap mass spectrometry analysis, the 35 kDa protein was identified as annexin V. We then studied the distribution of serum antibodies to annexin V in patients with peripheral neuropathy. We found serum positive antibodies to annexin V only in some patients with immune-mediated neuropathy. This indicated that humoral immune responses to annexin V might play a role in the pathogenesis of autoimmune sensory neuropathy or sensory neuronopathy.
Department of Molecular and Cellular Pharmacology, College of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100083, PR China.
Quan
Q
Li
Hongzhi
H
Guan
Xiaoping
X
Pu
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017304:Annexin A5 / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002648:Child; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009475:Neurons, Afferent / Q000737:chemistry*; D010523:Peripheral Nervous System Diseases / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020539:Sequence Analysis, Protein / Q000379:methods
D017304:Annexin A5