Combining a targeted radiotherapy and gene therapy approach for adenocarcinoma of prostate.
A targeted radiotherapy/gene therapy approach for prostate cancer, using the radiopharmaceutical [(131)I]meta-iodobenzylguanidine ([(131)I]MIBG), would restrict the effects of radiotherapy to malignant cells, thereby increasing efficacy and decreasing morbidity of radiotherapy. Prostate cancer cells were transfected with a transgene encoding the noradrenaline transporter (NAT) under the control of tumour-specific telomerase promoters, enabling them to actively take up [(131)I]MIBG. This led to tumour-specific cell kill. This strategy has the advantage of generating a radiological bystander effect, leading to the destruction of neighbouring tumour cells that have escaped transfection. This targeted approach could be a promising tumour-specific treatment option for prostate cancer.
Fullerton
N E
NE
Centre for Oncology & Applied Pharmacology, University of Glasgow, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK. n.fullerton@beatson.gla.ac.uk
Boyd
M
M
Mairs
R J
RJ
Keith
W N
WN
Alderwish
O
O
Brown
M M
MM
Livingstone
A
A
Kirk
D
D
D019797:3-Iodobenzylguanidine / Q000627:therapeutic use*; D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D024201:Bystander Effect; D003131:Combined Modality Therapy; D015316:Genetic Therapy; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D050484:Norepinephrine Plasma Membrane Transport Proteins; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D019275:Radiopharmaceuticals / Q000627:therapeutic use*; D027981:Symporters / Q000627:therapeutic use*; D019098:Telomerase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D007457:Iodine Radioisotopes; D050484:Norepinephrine Plasma Membrane Transport Proteins; D019275:Radiopharmaceuticals; C493227:SLC6A2 protein, human; D027981:Symporters; D019797:3-Iodobenzylguanidine; D019098:Telomerase