Partial liquid ventilation with FC-77 suppresses the release of lipid mediators in rat acute lung injury model.
OBJECTIVE
To investigate whether the release of lipid mediators is suppressed in rats with experimentally induced acute lung injury managed with partial liquid ventilation (PLV) using FC-77.


METHODS
Prospective, randomized controlled study.


METHODS
Research laboratory in a university.


METHODS
Male Sprague-Dawley rats.


METHODS
After tracheostomy was performed under general anesthesia, lung injury was induced by intratracheal instillation of HCl. The PLV group was then subjected to conventional gas ventilation for 30 mins, followed by PLV using FC-77. The control group was subjected to conventional gas ventilation throughout the study period.


RESULTS
In the PLV group the following results were obtained: a) impaired oxygenation was markedly improved; b) the increase in the serum levels of lipid mediators such as leukotriene B4, thromboxane A2, and 6-keto-prostaglandin F1alpha was suppressed; and c) the increase in the concentrations of leukotriene B4, thromboxane A2, and 6-keto-prostaglandin F1alpha in the total lung homogenate at 180 mins after lung injury was also suppressed.


CONCLUSIONS
This study indicates that PLV using FC-77 suppresses the release of lipid mediators in our rat model of acute lung injury. However, further investigation is needed to clarify the precise mechanism of this effect.
Hirayama
Yoh
Y
Department of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
Hirasawa
Hiroyuki
H
Oda
Shigeto
S
Shiga
Hidetoshi
H
Matsuda
Kenichi
K
Ueno
Hirokazu
H
Nakamura
Masataka
M
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis*; D005466:Fluorocarbons / Q000494:pharmacology* / Q000627:therapeutic use; D006851:Hydrochloric Acid / Q000009:adverse effects; D018836:Inflammation Mediators / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D021061:Liquid Ventilation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D013928:Thromboxane A2 / Q000096:biosynthesis
D001095:Arachidonic Acids; D005466:Fluorocarbons; D018836:Inflammation Mediators; C429307:fluorocarbon 77; D007975:Leukotriene B4; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D006851:Hydrochloric Acid