Labeling of major plant lipids and jasmonic acid using [1-(14C)] lauric acid.
A medium chain length fatty acid, [1-(14C)] lauric acid (12:0) was administered to the detached leaves of Artemisia and was incorporated into major lipids, including phospholipids and galactolipids. [1-(14C)]12:0 was elongated and desaturated into linolenic acid (18:3). In detached leaves of both Artemisia and Arabidopsis thaliana ecotype Columbia, radioactivity from [14C]18:3 was incorporated into jasmonic acid (JA) and methyl jasmonate (MJ). Higher amounts of [14C]JA were measured in Artemisia than Arabidopsis leaves. In Artemisia, [14C]JA was actively metabolized into [14C]MJ. Extracts prepared from the leaves of Artemisia, exhibited higher in vitro JA methyltransferase activity than those from Arabidopsis.
Afitlhile
Meshack M
MM
Department of Agronomy, University of Kentucky, 403 Plant Science Building, 1405 Veterans Drive, Lexington, KY 40546-0312, USA.
Fukushige
Hirotada
H
Hildebrand
David
D
D017360:Arabidopsis / Q000378:metabolism*; D019306:Artemisia / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003517:Cyclopentanes / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008738:Methyl Ethers / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D054883:Oxylipins; D018515:Plant Leaves / Q000378:metabolism
D002250:Carbon Radioisotopes; D003517:Cyclopentanes; D007850:Lauric Acids; D008055:Lipids; D008738:Methyl Ethers; D054883:Oxylipins; C030358:lauric acid; C011006:jasmonic acid