A new method of DNA preparation for pulsed-field gel electrophoresis analysis.
We have developed a new, labour-saving method of preparation, handling and treatment of DNA-containing agarose plugs for pulsed-field gel electrophoresis (PFGE). A plastic mould in which plugs are formed and supported during DNA purification and digestion was designed and successfully tested in a prototype device.
Fiett
Janusz
J
Centre for Quality Control in Microbiology, Chelmska 30/34, 00-725 Warsaw, Poland. fiett@cls.edu.pl
Kucharczyk
Krzysztof
K
Gniadkowski
Marek
M
D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*
D004269:DNA, Bacterial