Arecoline excites the colonic smooth muscle motility via M3 receptor in rabbits.
Arecoline is an effective component of areca (betel nuts, a Chinese medicine named pinang or binglang). The purpose of this study was to investigate the effect of arecoline on the motility of distal colon in rabbits and its mechanisms involved. Strips of colonic smooth muscle were suspended in organ baths containing Krebs solution, and their isometric contractions were examined. The response of smooth muscle to arecoline in colonic strips was recorded. The effects of atropine, gallamine and 1,1-dimethyl-4-diphenylacetoxypiperidiniumiodide (4-DAMP) on arecoline-induced contraction were also observed. Arecoline (1 nM - 1 microM) produced a concentration-dependent contraction in both the longitudinal and the circular smooth muscle of rabbit colon. Atropine (10 microM) abolished the arecoline (80 nM)--induced contraction. M3 receptor antagonist, 4 - DAMP (0.4 microM), abolished the arecoline (80 nM)--related response, whereas M2 receptor antagonist, gallamine (0.4 microM), did not affect the effect of arecoline. These results suggest that arecoline excites the colonic motility via M3 receptor in rabbits.
Institute of Physiology, School of Medicine, Shandong University, Jinan 250012, P R China. xiedping@sdu.edu.cn
Dong-Ping
DP
Xie
Lian-Bi
LB
Chen
Chuan-Yong
CY
Liu
Chun-Ling
CL
Zhang
Ke-Jing
KJ
Liu
Paulus S
PS
Wang
D000818:Animals; D001115:Arecoline / Q000494:pharmacology*; D001285:Atropine / Q000494:pharmacology; D003106:Colon / Q000187:drug effects* / Q000294:innervation; D004305:Dose-Response Relationship, Drug; D005260:Female; D005731:Ganglionic Stimulants / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology; D011817:Rabbits; D043585:Receptor, Muscarinic M2 / Q000187:drug effects; D043587:Receptor, Muscarinic M3 / Q000187:drug effects*
D005731:Ganglionic Stimulants; D018727:Muscarinic Antagonists; D010880:Piperidines; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D001115:Arecoline; D001285:Atropine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium