17beta-hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor alpha-regulated gene in mouse intestine.
In order to study the role of peroxisome proliferator-activated receptor alpha in mouse intestine, its agonist-induced proteins were identified by peptide mass fingerprinting followed by Northern blot analysis using their cDNAs. One of the most remarkably induced proteins was identified as 17beta-hydroxysterol dehydrogenase type 11. Its very rapid induction by various agonists was most efficient in intestine and then in liver. These findings together with recently reported results showing the enzyme family's wide substrate spectrum, including not only glucocorticoids and sex steroids but also bile acids, fatty acids and branched chain amino acids, suggest new roles for both peroxisome proliferator-activated receptor alpha and 17beta-hydroxysterol dehydrogenase type 11 in lipid metabolism and/or detoxification in the intestine.
Department of Biochemistry, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. motojima@my-pharm.ac.jp
Kiyoto
K
Motojima
D015067:17-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D004790:Enzyme Induction / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D047493:PPAR alpha / Q000819:agonists / Q000172:deficiency / Q000502:physiology*; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping / Q000379:methods; D020025:Peroxisome Proliferators / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007527:Isoenzymes; D047493:PPAR alpha; D010446:Peptide Fragments; D020025:Peroxisome Proliferators; D011743:Pyrimidines; D012333:RNA, Messenger; C006253:pirinixic acid; D015067:17-Hydroxysteroid Dehydrogenases