Interaction of non-ionic surfactants with hepatic CYP in Prochilodus scrofa.
Cytochromes P450 (CYP) constitute a superfamily of hemeproteins that play a vital role in the metabolism of a wide variety of endogenous and xenobiotic compounds. Xenobiotic metabolism and the role of CYP are of particular interest in studies regarding the prevention of the damage caused by chemical pollutants. We investigated, in this study, the interaction of Triton X-100 and Tween 80 with CYP and antioxidant defenses in Curimbata, a Brazilian fish. Aiming to clarify the effects of non-ionic surfactants in the monooxigenase system of fish through in vitro study, the effects of Triton X-100 and Tween 80 were analyzed using monooxygenases and antioxidant system as experimental model. Total CYP and EROD were strongly inhibited by Triton X-100 and Tween 80 in a concentration-dependent way; the content of CYP was reduced until zero while EROD activity was completely inhibited in the presence of Triton X-100 and more than 40% inhibited in the presence of Tween 80. Each surfactant causes a different effect on each antioxidant enzyme. No effect was detected in SOD activity in the presence of even Triton X-100 or Tween 80. Triton X-100 increase catalase activity, while Tween 80 decreases this enzyme activity. The molecular structure of the surfactants causes the alteration of this system, since they are able to interact with the microsomal protein, especially with monooxigenase's components, altering their conformation and, consequently destroying their function. Our results suggest that surfactants can interact with components of the microsomal system leading to inhibition of CYP. Therefore, CYP activity, which has been used as a biomarker of xenobiotic exposure, should be used as a marker in association with other enzymes.
Laboratory of Biomembranes, Department of Biochemistry, Institute of Biology, State University of Campinas, CP6109, ZIP CODE 13083-970, Cidade Universitária Zeferino Vaz, Barão Geraldo, Campinas, SP, Brazil. feracin@unicamp.br
Maria E F
ME
da Silva
Nilce C
NC
Meirelles
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000494:pharmacology*; D005399:Fishes / Q000502:physiology; D008099:Liver / Q000201:enzymology; D017830:Octoxynol / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000494:pharmacology*; D015262:Xenobiotics / Q000378:metabolism*
D015415:Biomarkers; D011136:Polysorbates; D013501:Surface-Active Agents; D015262:Xenobiotics; D017830:Octoxynol; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1