Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel.
OBJECTIVE
The mucosa associated flora of the large intestine is important in determining mucosal function although what controls its composition is unknown. This study has determined the effect of the prebiotic carbohydrates oligofructose and inulin on the mucosal flora.


METHODS
An in vitro chemostat model of both planktonic and surface associated bacteria was used followed by an intervention study in 29 subjects undergoing colonoscopy.


METHODS
Fourteen subjects, recruited from colonoscopy waiting lists, supplemented their diet for two weeks with a mix of 7.5 g of oligofructose and 7.5 g inulin. Fifteen subjects were recruited at the time of colonoscopy and given no supplement. Multiple endoscopic biopsies were taken from the caecum, transverse and descending colon, and rectum. The mucosal flora was characterised by culture and to species level by cellular fatty acid profiles. Cell proliferation was assessed by immunohistochemical staining for minichromosome maintenance protein 2, Ki67, and proliferating cell nuclear antigen.


RESULTS
In vitro prebiotics increased surface counts of bifidobacteria from 6.6 to 7.3 log(10) colony forming units (CFU) per slide (p<0.0006) with no significant changes in planktonic bacteria. In the feeding study, prebiotics increased mucosal bifidobacteria (log CFU/g mucosa (SEM)) in both the proximal (control 5.3 (0.4) v prebiotic 6.3 (0.3)) (p = 0.059) and distal (control 5.2 (0.3) v prebiotic 6.4 (0.3)) colon (p = 0.01). Lactobacilli were also increased (3.0 (0.1) v 3.7 (0.2) (p = 0.02) in the proximal and 3.1 (0.1) v 3.6 (0.2) (p = 0.04) in the distal colon, respectively). There were significantly more eubacteria in fed subjects but no changes in total anaerobes clostridia, bacteroides, or coliforms, nor in proliferation indices.


CONCLUSIONS
Prebiotic carbohydrates can change the composition of the mucosa associated flora significantly.
Department of Gastroenterology, Addenbrookes Hospital, Cambridge, UK.
S J
SJ
Langlands
M J
MJ
Hopkins
N
N
Coleman
J H
JH
Cummings
D000328:Adult; D000368:Aged; D001644:Bifidobacterium / Q000254:growth & development; D002455:Cell Division / Q000187:drug effects; D003113:Colonoscopy; D015169:Colony Count, Microbial; D019587:Dietary Supplements / Q000009:adverse effects; D005243:Feces / Q000382:microbiology; D005260:Female; D005414:Flatulence / Q000209:etiology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology; D007420:Intestine, Large / Q000166:cytology / Q000382:microbiology*; D007444:Inulin / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009844:Oligosaccharides / Q000009:adverse effects / Q000494:pharmacology*; D019936:Probiotics
D009844:Oligosaccharides; C120489:oligofructose; D007444:Inulin