Airway epithelial cell-pathogen interactions.
Cystic fibrosis (CF) airway becomes colonized with only a limited number of bacterial pathogens. It is of paramount importance to establish in vitro and in vivo models to better understand bacterial-host interactions under CF-like conditions. In this article, in vitro methods suitable to study Pseudomonas aeruginosa (Pa) and Staphylococcus aureus (Sa) adherence to and uptake by airway epithelial cells are described. Acute and chronic respiratory infection models, which have been used in CF transgenic mice and mimic human CF lung pathology, are also taken into consideration.
Institut fur Allgemeine Hygiene und Umwelthygiene, Universität Tübingen, Tübingen, Germany.
Alessandra
A
Bragonzi
Elena
E
Copreni
Sophie
S
de Bentzmann
Martina
M
Ulrich
Massimo
M
Conese
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001422:Bacterial Adhesion / Q000502:physiology; D001424:Bacterial Infections / Q000503:physiopathology*; D018929:Cell Culture Techniques; D003550:Cystic Fibrosis / Q000503:physiopathology; D053938:DNA Fragmentation / Q000502:physiology; D004705:Endocytosis / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D020545:Respiratory Mucosa / Q000502:physiology* / Q000503:physiopathology; D012141:Respiratory Tract Infections / Q000503:physiopathology*; D013211:Staphylococcus aureus / Q000502:physiology*