A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures.
The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs)--polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3HB-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of 3H-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar.
Institute of Biophysics, The Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russia.
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EI
Shishatskaya
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TG
Volova
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival / Q000502:physiology*; D005720:Gamma Rays; D006358:Hot Temperature; D006885:Hydroxybutyrates / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D013499:Surface Properties; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C111948:poly(hydroxyethanoate); C003182:poly-beta-hydroxybutyrate