Activation of lignin peroxidase in organic media by reversed micelles.
Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined in this study; however, LIP was catalytically active by being entrapped in the AOT reversed micellar solution. LIP activity in the reversed micelle was enhanced by optimizing either the preparation or the operation conditions, such as water content and pH in water pools of the reversed micelle and the reaction temperature. Stable activity was obtained in isooctane because of the stability of the reversed micelle. The optimal pH was 5 in the reversed micellar system, which shifted from pH 3 in the aqueous solution. The degradation reaction of several environmental pollutants was attempted using LIP hosted in the AOT reversed micelle. Degradation achieved after a 1-h reaction reached 81%, 50%, and 22% for p-nonylphenol, bisphenol A, and 2,4-dichlorophenol, respectively. This is the first report on the utilization of LIP in organic media.
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki Fukuoka 812-8581, Japan.
Masayuki
M
Kimura
Junji
J
Michizoe
Shin-Ya
SY
Oakazaki
Shintaro
S
Furusaki
Masahiro
M
Goto
Hiroo
H
Tanaka
Hiroyuki
H
Wariishi
D003102:Colloids / Q000737:chemistry*; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D009930:Organic Chemicals / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
D003102:Colloids; D008823:Micelles; D009930:Organic Chemicals; D010636:Phenols; D012997:Solvents; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D010544:Peroxidases; C042858:lignin peroxidase