[Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection].
OBJECTIVE
To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances.


METHODS
Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro, on which the antiangiogenic effects of genistein, tyrphostin A23 and lavendustin C were assessed.


RESULTS
HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture, and was easily reproducible. Tyrosine kinase inhibitors, genistein, tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation.


CONCLUSIONS
This new human angiogenesis model has proved to be simple, responsive, and reproducible for testing potential angiogenic inhibitors, and is suitable for screening anti-angiogenic drugs.
School of Pharmacy, Yunnan University, Kunming 650091, China. libin36@ynu.edu.cn
Bin
B
Li
D020533:Angiogenesis Inhibitors; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology*; D019833:Genistein / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D020032:Tyrphostins / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D020032:Tyrphostins; D019833:Genistein; D011505:Protein-Tyrosine Kinases; C060641:tyrphostin A23