Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.
We found that the recombinant endo-beta-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside) in a good yield of 43%.
The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.
Kenji
K
Osumi
Yoshitaka
Y
Makino
Eri
E
Akaike
Takashi
T
Yamanoi
Mamoru
M
Mizuno
Midori
M
Noguchi
Toshiyuki
T
Inazu
Kenji
K
Yamamoto
Kiyotaka
K
Fujita
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D000118:Acetylglucosaminidase / Q000378:metabolism*; D003001:Cloning, Molecular; D006020:Glycopeptides / Q000378:metabolism*; D006031:Glycosylation; D009089:Mucor / Q000201:enzymology*; D009844:Oligosaccharides / Q000378:metabolism*; D010047:Ovalbumin; D011994:Recombinant Proteins
D006020:Glycopeptides; D009844:Oligosaccharides; D011994:Recombinant Proteins; C014124:4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside; D010047:Ovalbumin; D000118:Acetylglucosaminidase; D000117:Acetylglucosamine