CFTR, investigated with the two-electrode voltage-clamp technique: the importance of knowing the series resistance.
When the gene mutated in cystic fibrosis (CF) was identified and named the cystic fibrosis transmembrane conductance regulator (CFTR), it was not immediately clear what its function might be. Subsequent analysis showed that it is an anion channel, but also other functions were proposed. This protocol aims to shortly describe the two-electrode voltage-clamp (TEVC) technique and to highlight some precautions when studying CFTR with it. A short description of the series resistance (RS) and its influence on measurements of channel characteristics is given.
Nagel
Georg
G
Max-Planck-Institute of Biophysics, Marie-Curie-Str. 15, D-60439 Frankfurt/Main, Germany. Georg.Nagel@mpibp-frankfurt.mpg.de
D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology*; D017097:Electric Impedance; D006801:Humans; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods*
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator