[Simultaneous determination of residual fourteen kinds of beta-lactam and macrolide antibiotics in bovine muscles by high-performance liquid chromatography with a diode array detector].
Residues of 14 kinds of beta-lactam and macrolide antibiotics in bovine muscles were extracted with acetonitrile and the extract was partitioned with n-hexane to remove fat. High-performance liquid chromatography was carried out on a TSK-gel ODS-80TM column using gradient elution with acetonitrile-0.05% trifluoroacetic acid and the drugs were quantitated by diode array detection. The recoveries of the drugs from bovine muscles spiked at 0.1 ppm were over 63% and each quantitation limit was 0.04 ppm.
Chiba Prefectural Institute of Public Health, 666-2, Nitona-cho, Chuo-ku Chiba 260-8715, Japan.
Tomoko
T
Nagata
Eiichi
E
Ashizawa
Hiroyuki
H
Hashimoto
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D018942:Macrolides / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D047090:beta-Lactams / Q000032:analysis*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D047090:beta-Lactams