Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms.
The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei.
Capparelli
R
R
Zootechnical Sciences and Food Inspection Department, School of Agriculture, University of Naples, Federico II, Via Università 133, Portici, 80055 Naples, Italy. capparel@unina.it
Viscardi
M
M
Amoroso
M G
MG
Blaiotta
G
G
Bianco
M
M
D002957:Citrus / Q000145:classification* / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D005838:Genotype; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D021161:Self-Sustained Sequence Replication / Q000379:methods*
D018744:DNA, Plant; D005819:Genetic Markers