Isolation and characterization of limonoate and nomilinoate A-ring lactones.
A method combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the isolation of two key metabolites in the limonoid biosynthetic pathway critical to citrus quality. Potassium salts of limonoate A-ring lactone and nomilinoate A-ring lactone were isolated from young Chandler pummelo seedlings and characterized on the basis of proton and carbon NMR data.
United States Department of Agriculture, Western Regional Research Center, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA.
Audrius A
AA
Zukas
Andrew P
AP
Breksa
Gary D
GD
Manners
D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry* / Q000378:metabolism; D007783:Lactones / Q000737:chemistry* / Q000302:isolation & purification*; D036701:Limonins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
D007783:Lactones; D036701:Limonins