[Role of pancreatic enzymes in gut injury secondary to trauma/hemorrhagic shock in rats].
OBJECTIVE
To test the hypothesis that pancreatic digestive enzymes are involved in trauma/hemorrhagic shock (T/HS)-induced intestinal injury.


METHODS
Male Sprague-Dawley rats were given pancreatic enzyme inhibitor (6-amidino-2 -naphthyl p-guanidinobenzoate dimethanesulfonate, ANGD) intraluminally or intravenously after laparotomy (trauma), and then subjected to 90 min of hemorrhagic shock or sham shock. Intestinal injury was assessed 3 h after resuscitation with Ringer's lactate solution.


RESULTS
Histological analysis demonstrated fewer injured villi in shock rats with intraluminal ANGD treatment than in those with intravenous ANGD administration and control shock rats. Furthermore, intestinal intraluminal perfusion of Ringer's lactate solution without ANGD produced a certain degree of protection against T/HS-induced intestinal injury, as compared with the rats without perfusion.


CONCLUSIONS
Pancreatic proteolytic enzymes in ischemic gut may serve as important toxic factors contributing to gut injury following T/HS.
Shi
Han-ping
HP
Department of Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shihp38@hotmail.com
Wen
Ying
Y
Zhang
Ce
C
Xue
Qi
Q
Lei
Shang-tong
ST
D000818:Animals; D001550:Benzamidines; D006146:Guanidines / Q000494:pharmacology; D007422:Intestines / Q000473:pathology*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000201:enzymology / Q000209:etiology*; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors; D014947:Wounds and Injuries / Q000150:complications* / Q000201:enzymology
D001550:Benzamidines; D006146:Guanidines; D014361:Trypsin Inhibitors; D014357:Trypsin; C032855:nafamostat