A role for mannose-binding lectin dysfunction in generation of autoantibodies in systemic lupus erythematosus.
OBJECTIVE
To investigate the possible association of the mannose binding lectin (MBL) pathway of complement activation with different disease parameters and disease activity in patients with systemic lupus erythematosus (SLE).


METHODS
MBL genotype, MBL serum concentration, MBL complex activity and MBL pathway activity were assessed in 53 patients. The activity of the MBL-MASP complex was assessed on the basis of its ability to activate exogenous C4. For MBL pathway activity the formation of the terminal complex of complement activation (C5b-9) was measured. Results were analysed in relation to clinical variables and autoantibody profiles in these patients.


RESULTS
MBL complex activity and MBL pathway activity were both reduced in patients carrying MBL variant alleles. Anticardiolipin and anti-C1q autoantibodies were observed significantly more frequently in patients with MBL variant alleles. Furthermore, the presence of these autoantibodies was associated with a decreased MBL concentration and function. In contrast, anti-MBL autoantibodies were not found in patients with MBL variant alleles, possibly related to impaired binding of variant MBL to apoptotic material.


CONCLUSIONS
In patients with SLE, a reduced functional activity of the MBL pathway of complement, in relation to expression of MBL variant alleles, is associated with increased levels of autoantibodies against cardiolipin and C1q, but not against MBL. We hypothesize that an enhanced production of autoantibodies may be related to disturbed clearance of apoptotic material due to impaired MBL function.
Department of Nephrology, C3P-29, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands. majseelen@lumc.nl
M A
MA
Seelen
E A
EA
van der Bijl
L A
LA
Trouw
T C M
TC
Zuiverloon
J R
JR
Munoz
F C
FC
Fallaux-van den Houten
N
N
Schlagwein
M R
MR
Daha
T W J
TW
Huizinga
A
A
Roos
D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000097:blood; D001323:Autoantibodies / Q000096:biosynthesis*; D003167:Complement Activation; D015922:Complement C1q / Q000276:immunology; D005260:Female; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology*; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011110:Polymorphism, Genetic; D012720:Severity of Illness Index
D017153:Antibodies, Anticardiolipin; D001323:Autoantibodies; D037601:Mannose-Binding Lectin; D015922:Complement C1q