Visual stimulation, 1H MR spectroscopy and fMRI of the human visual pathways.
The purpose was to assess changes in lactate content and other brain metabolites under visual stimulation in optical chiasm, optic radiations and occipital cortex using multiple voxel MR spectroscopy (MRS). 1H chemical shift imaging (CSI) examinations of transverse planes centered to include the above structures were performed in four subjects at an echo time of 135 ms. Functional MRI (fMRI) was used to confirm the presence of activity in the visual cortex during the visual stimulation. Spectral maps of optical chiasm were of poor quality due to field disturbances caused by nearby large blood vessels and/or eye movements. The optic radiations and the occipital lobe did not show any significant MR spectral change upon visual stimulation, i.e., the peak areas of inositol, choline, creatine, glutamate and N-acetylaspartate were not affected. Reproducible lactate signals were not observed. fMRI confirmed the presence of strong activations in stimulated visual cortex. Prolonged visual stimulation did not cause significant changes in MR spectra. Any signal observed near the 1.33 ppm resonance frequency of the lactate methyl-group was artifactual, originating from lipid signals from outside the volume of interest (VOI). Previous claims about changes in lactate levels in the visual cortex upon visual stimulation may have been based on such erroneous observations.
Laboratory for Experimental Ophthalmology, University of Groningen, Postbus 30001, Groningen, 9700 RB, The Netherlands.
Christine C
CC
Boucard
Jop P
JP
Mostert
Frans W
FW
Cornelissen
Jacques
J
De Keyser
Matthijs
M
Oudkerk
Paul E
PE
Sijens
D000328:Adult; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001931:Brain Mapping; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010775:Photic Stimulation; D014793:Visual Cortex / Q000378:metabolism / Q000502:physiology*; D014795:Visual Pathways / Q000378:metabolism / Q000502:physiology*
D007773:Lactates; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline