Synthesis of the Lewis b hexasaccharide and HSA-conjugates thereof.
An efficient and short route has been elaborated for the aminopropyl spacer equipped Leb hexasaccharide. For the preparation of HSA-conjugates of this oligosaccharide, the use of disuccinimidyl suberate (DSS) and disuccinimidyl glutarate (DSG) as cross-linker reagents has been evaluated. This conjugation method emerged as being faster and easier to monitor by standard MALDI-TOF spectrometry than squarate ester based conjugations of similar efficiency if DSS is used as cross-linker.
Department of Chemistry, Göteborg University, S-412 96 Gothenburg, Sweden. martina.lahmann@chem.gu.se
Martina
M
Lahmann
Linnéa
L
Bülow
Peter
P
Teodorovic
Helena
H
Gybäck
Stefan
S
Oscarson
D003432:Cross-Linking Reagents; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013388:Succinimides
C500822:3-aminopropyl fucopyranosyl-1-2-galactopyranosyl-1-3-(fucopyranosyl-1-4)-2-acetamido-2-deoxyglucopyranosyl-1-3-galactopyranosyl-1-4-glucopyranoside; D003432:Cross-Linking Reagents; D006001:Glycoconjugates; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D012709:Serum Albumin; D013388:Succinimides; C096464:disuccinimidyl glutarate; C019358:disuccinimidyl suberate