Brainstem prolactin mRNA is enhanced in mice with suppressed neuronal nitric oxide synthase activity.
Prolactin (PRL) and vasoactive intestinal polypeptide (VIP) mRNA levels were elevated in the brainstem of neuronal nitric oxide synthase (nNOS) gene knockout (KO) mice compared to the levels in nNOS control mice. In addition, PRL mRNA levels increased in the hypothalamus and the brainstem of nNOS control mice after administration of 7-nitro-indazole (7-NI), a relatively selective nNOS inhibitor. The results suggest that NO inhibits PRL. No differences in the genes measured were observed in inducible NOS KO mice.
Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University, 205 Wegner Hall, P.O. Box 646520, Pullman, WA 99164-6520, USA.
Lichao
L
Chen
Ping
P
Taishi
Deborah
D
Duricka
James M
JM
Krueger
D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism; D007191:Indazoles / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D011388:Prolactin / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D007191:Indazoles; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse; C080122:7-nitroindazole