Genotoxicity of aspartame.
In the present study, the genotoxic effects of the low-calorie sweetener aspartame (ASP), which is a dipeptide derivative, was investigated using chromosome aberration (CA) test, sister chromatid exchange (SCE) test, micronucleus test in human lymphocytes and also Ames/Salmonella/ microsome test. ASP induced CAs at all concentrations (500, 1000 and 2000 microg/ml) and treatment periods (24 and 48 h) dose-dependently, while it did not induce SCEs. On the other hand, ASP decreased the replication index (RI) only at the highest concentration for 48 h treatment period. However, ASP decreased the mitotic index (MI) at all concentrations and treatment periods dose-dependently. In addition, ASP induced micronuclei at the highest concentrations only. This induction was also dose-dependent for 48 hours treatment period. ASP was not mutagenic for Salmonella typhimurium TA98 and TA100 strains in the absence and presence of S9 mix.
Rencüzoğullari
Eyyüp
E
Biology Department, Faculty of Arts and Sciences, Natural and Applied Sciences Institute, Cukurova University, Adana, Turkey. reyyup@mail.cu.edu.tr
Tüylü
Berrin Ayaz
BA
Topaktaş
Mehmet
M
Ila
Hasan Basri
HB
Kayraldiz
Ahmet
A
Arslan
Mehmet
M
Diler
Songül Budak
SB
D000818:Animals; D001218:Aspartame / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens; D051381:Rats; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013549:Sweetening Agents / Q000633:toxicity*
D009153:Mutagens; D013549:Sweetening Agents; D001218:Aspartame