[A new method for screening mutant yeast strains with green fluorescent protein].
OBJECTIVE
To establish a fast and simple method for screening mutant yeast strains.


METHODS
Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library, with the green fluorescent protein gene as the reporter gene in the transposon.


RESULTS
The strains that emitted green fluorescence were isolated, indicating that the gfp gene was inserted into the yeast genome by homologous recombination.


CONCLUSIONS
This study established a useful method for functional genome study by homologous recombination technique, and provide an alternative for gene therapeutic drug development.
Hu
Lian-mei
LM
Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. hulianmei@yahoo.com.cn
Piao
Ying-jie
YJ
He
Cai-gu
CG
Ma
Wen-li
WL
Cui
Dong
D
Zheng
Weng-ling
WL
D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic; D015003:Yeasts / Q000235:genetics*
D049452:Green Fluorescent Proteins