Neurogranin expression in stably transfected N2A cell line affects cytosolic calcium level by nitric oxide stimulation.
To test a cellular effect of rodent neurogranin (Ng) oxidation as compared to Ng phosphorylation, we develop a cell model capable of stable expression of Ng using the Tet-On system, and determine whether Ng oxidation regulates intracellular calcium level. Our results show that Ng oxidation by nitric oxide donor induces an increase of [Ca(2+)](i) in Ng-expressed cells as compared to the control cells without expressing Ng. These results suggest that Ng oxidation plays a significant role in intracellular Ca(2+) homeostasis, essential for the activated signaling networks in learning and memory.
Yang
Hui Ming
HM
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Lee
Psyche Huu-Hoa
PH
Lim
Tit Meng
TM
Sheu
Fwu-Shan
FS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006706:Homeostasis; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D051579:Neurogranin; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; C496650:Nrgn protein, mouse; D051579:Neurogranin; D009569:Nitric Oxide; D002118:Calcium