Molecular characterization of an Infectious bursal disease virus isolate from Iran.
The segment A of an Infectious bursal disease virus (IBDV) isolate from Iran was amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced and compared with published sequences of 26 IBDV isolates from other parts of the world. The Iranian isolate showed 8 unique amino acid differences. In addition, 9 common amino acid differences, namely 3 in VP2, (222 Ala, 256 lIe and 294 lIe), 3 in VP4 (685 Asn/Ser, 715 Ser and 751 Asp), 2 in VP3 (990 Val and 1005 Ala), and 1 in VP5 (49 Arg) were found. Phylogenetic analysis indicated that the Iranian isolate is closely related to highly virulent (hv) IBDV isolates from Asian countries. Nevertheless, it may share a common origin with hv isolates from other parts of the world.
Research Center of Animal Science and Natural Resources, Arak, Iran.
S D
SD
Hosseini
A R
AR
Omar
I
I
Aini
D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary / Q000821:virology; D002645:Chickens / Q000821:virology; D018076:DNA, Complementary / Q000737:chemistry; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007492:Iran; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
D018076:DNA, Complementary; D012367:RNA, Viral; C082513:VP2 protein, infectious bursal disease virus; C478244:VP3 protein, infectious bursal disease virus; C478245:VP4 protein, infectious bursal disease virus; D015678:Viral Structural Proteins