A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.
Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
Gómez
Eliana B
EB
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Angeles
Vanessa T
VT
Forsburg
Susan L
SL
D000483:Alleles; D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; D002453:Cell Cycle; D019926:Cyclin B / Q000172:deficiency; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005816:Genetic Complementation Test; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D010957:Plasmids; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D011503:Transglutaminases / Q000737:chemistry / Q000235:genetics
D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; C577398:CUT9 protein, S pombe; D019926:Cyclin B; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C082463:RAD4 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D011503:Transglutaminases