Antibodies for CFTR studies.
For most expression studies focusing on the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, sensitive and specific antibodies (Abs) are critically needed. Several Abs have been produced commercially or by research laboratories for CFTR detection in both cell lines with heterologous or endogenous expression and native cells/tissues. Here, we review the applicability of most Abs currently in use in CF research for the biochemical and/or immunocytochemical detection of CFTR.
Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Lisboa, Portugal.
Filipa
F
Mendes
Carlos M
CM
Farinha
Mónica
M
Roxo-Rosa
Pascale
P
Fanen
Aleksander
A
Edelman
Robert
R
Dormer
Margaret
M
McPherson
Heather
H
Davidson
Edith
E
Puchelle
Hugo
H
De Jonge
Ghanshyam D
GD
Heda
Martina
M
Gentzsch
Gergely
G
Lukacs
Deborah
D
Penque
Margarida D
MD
Amaral
D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis* / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques
D000906:Antibodies; D000911:Antibodies, Monoclonal; C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator