Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.
Institute of Food and Agricultural Technology, University of Girona, Campus Montilivi s/n, E-17071 Girona, Spain.
David
D
Rodríguez-Lázaro
Anna
A
Jofré
Teresa
T
Aymerich
Marta
M
Hugas
Maria
M
Pla
D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008461:Meat Products / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D034421:Sus scrofa
D004269:DNA, Bacterial