Par6alpha signaling controls glial-guided neuronal migration.
Neuronal migrations along glial fibers provide a primary pathway for the formation of cortical laminae. To examine the mechanisms underlying glial-guided migration, we analyzed the dynamics of cytoskeletal and signaling components in living neurons. Migration involves the coordinated two-stroke movement of a perinuclear tubulin 'cage' and the centrosome, with the centrosome moving forward before nuclear translocation. Overexpression of mPar6alpha disrupts the perinuclear tubulin cage, retargets PKCzeta and gamma-tubulin away from the centrosome, and inhibits centrosomal motion and neuronal migration. Thus, we propose that during neuronal migration the centrosome acts to coordinate cytoskeletal dynamics in response to mPar6alpha-mediated signaling.
Solecki
David J
DJ
Laboratory of Developmental Neurobiology, The Rockefeller University, New York, New York 10021, USA.
Model
Lynn
L
Gaetz
Jedidiah
J
Kapoor
Tarun M
TM
Hatten
Mary E
ME
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology; D022081:Cell Surface Extensions / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology*; D003001:Cloning, Molecular / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D003952:Diagnostic Imaging / Q000379:methods; D000072159:Dynactin Complex; D005822:Genetic Vectors / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000821:virology; D009924:Organ Culture Techniques; D038761:Photobleaching; D011493:Protein Kinase C / Q000378:metabolism; D051744:Protein Kinase C-epsilon; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016337:RNA, Catalytic / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism; D014743:Videotape Recording / Q000379:methods; D015215:Zidovudine / Q000378:metabolism
D000072159:Dynactin Complex; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D011506:Proteins; D016337:RNA, Catalytic; D014404:Tubulin; C418481:hairpin ribozyme; D049452:Green Fluorescent Proteins; D015215:Zidovudine; C497256:Prkce protein, mouse; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon