Induction of the anticarcinogenic marker enzyme, quinone reductase, by Dalbergiae Lignum.
The effect of an extract of Dalbergiae Lignum and four components that were isolated from the extract on the anticarcinogenic phase II marker enzyme, quinone reductase (QR), was investigated. Of the solvent extracts of Dalbergiae Lignum, the CH2Cl2 fraction was the most potent in inducing QR activity, with a CD value (the concentration required to double the QR activity) of 29.5 microg/mL. The CH2Cl2 extract was further separated into six compounds, four of which were identified as 4-methoxydalbergione, latifolin, 4',6-dihydroxy-7-methoxyflavanone, and obtusafuran. Obtusafuran [CD = 1.1 microM; chemopreventive index (CI) = 101.9] and latifolin (CD = 1.7 microM; CI = 154.6) displayed potent QR inducing activity and high chemopreventive indices. Latifolin and 4-methoxydalbergione were identified as strong DPPH-scavengers with half-maximal free radical scavenging concentrations of 15.9 and 17.2 microM, respectively.
College of Pharmacy and MeRRI, Wonkwang University, Iksan 570-749 Korea.
Hu-Quan
HQ
Yin
Bang-Wool
BW
Lee
Youn-Chul
YC
Kim
Dong-Hwan
DH
Sohn
Byung-Hoon
BH
Lee
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D035782:Dalbergia; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D010936:Plant Extracts; D016660:NAD(P)H Dehydrogenase (Quinone)