Simultaneous analysis of piperacillin and tazobactam in rabbits: application to pharmacokinetic study.
A simple and rapid assay is developed for the simultaneous analysis of piperacillin and tazobactam in rabbit serum and tissue cage fluid (TCF). To eliminate endogenous interferences, a wavelength switch technique was applied, in which the programmable UV detector changed the monitoring wavelength from 218 to 254 nm at 10 min. After liquid-liquid extraction, sample analyses were performed on a C(18) column by gradient elution; the mobile phase consisted of acetonitrile and phosphate buffer (0.014 m, pH 2.4). Owing to the limited amount of rabbit TCF available, a cross-validation of a proxy matrix was evaluated. The relative standard deviation of the between- and within-batch precision of both compounds was less than 5.1%; the relative error of the between- and within-batch accuracy was less than 7.3%. The recoveries of both compounds in serum and TCF were larger than 80%. This assay was successfully applied to simultaneously analyze piperacillin and tazobactam in rabbit serum and TCF samples.
Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, USA. cli@hartshosp.org
Chonghua
C
Li
Dawei
D
Xuan
Ming
M
Ye
Charles H
CH
Nightingale
David P
DP
Nicolau
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D015194:Diffusion Chambers, Culture; D005260:Female; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D010878:Piperacillin / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D000078142:Tazobactam
D010397:Penicillanic Acid; D000078142:Tazobactam; D010878:Piperacillin