Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay.
The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.
Adachi
D
D
Division of Microbiology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont., Canada M5G 1X8.
Johnson
G
G
Draker
R
R
Ayers
M
M
Mazzulli
T
T
Talbot
P J
PJ
Tellier
R
R
D001483:Base Sequence; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D028941:Coronavirus 229E, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D028962:Coronavirus OC43, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology
D018076:DNA, Complementary; D004279:DNA, Viral; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific