Extracellular matrix-like surfactant polymers containing arginine-glycine-aspartic acid (RGD) peptides.
We report on a novel series of biomimetic polymers exhibiting interfacial properties similar to the extracellular matrix. A series of well-defined surfactant polymers were synthesized by simultaneously incorporating arginine-glycine-aspartic acid (RGD) peptide, dextran oligosaccharide, and hexyl ligands with controlled feed ratios onto a poly(vinyl amine) (PVAm) backbone. The peptide sequence was H-GSSSGRGDSPA-NH(2) (Pep) having a hydrophilic extender at the amino terminus and capped carboxy terminus. The peptide-to-dextran (Pep:Dex) ratios were varied to create surfactants having 0, 25, 50, 75, and 100 mol-% peptide relative to dextran. The surfactants were characterized by IR, NMR and atomic force microscopy (AFM) for composition and surface active properties. AFM confirmed full surface coverage of PVAm(Pep)(100%) on graphite, and supported the mechanism of interdigitation of hexyl ligands between surfactant molecules within a specified range of hexyl chain densities. the attachment and growth of human pulmonary artery endothelial cells on the PVAm(Pep)(100%) surface was identical to the fibronectin positive control. Cell adhesion decreased dramatically with decreasing peptide density on the surfactant polymers. Molecular model of a peptide surfactant polymer, consisting of poly(vinyl amine) backbone with peptide, dextran oligosaccharide and hexyl branches coupled to the polymer chain.
Anderson
Eric H
EH
Department of Macromolecular Science, Wickenden 201, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-7207, USA.
Ruegsegger
Mark A
MA
Murugesan
Gurunathan
G
Kottke-Marchant
Kandice
K
Marchant
Roger E
RE
D000042:Absorption; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002448:Cell Adhesion; D002455:Cell Division; D042783:Endothelial Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D011651:Pulmonary Artery / Q000166:cytology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
D009842:Oligopeptides; D011108:Polymers; D013501:Surface-Active Agents; C047981:arginyl-glycyl-aspartic acid