Biosynthesis and compositional regulation of poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in recombinant ralstonia eutropha expressing mutated polyhydroxyalkanoate synthase genes.
A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB(-)4 harboring mutated PHA synthase genes (phaC(Ac)) from Aeromona caviae was investigated. The strain harboring wild-type phaC(Ac) gene produced a PHA copolymer consisting of (R)-3-hydroxybutyrate and (R)-3-hydroxyhexanoate [P(3HB-co-3HHx)] with 3.5 mol-% of 3HHx fraction from soybean oil. When the mutants of phaC(Ac) gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0-5.1 mol-%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes.
Tsuge
Takeharu
T
Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatuta, Midori-ku, Yokohama 226-8502, Japan. ttsuge@iem.titech.ac.jp
Saito
Yu
Y
Kikkawa
Yoshihiro
Y
Hiraishi
Tomohiro
T
Doi
Yoshiharu
Y
D020155:3-Hydroxybutyric Acid / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000333:Aeromonas / Q000201:enzymology* / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D002208:Caproates / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D020118:Cupriavidus necator / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics; D019020:Directed Molecular Evolution; D005285:Fermentation / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D000217:Acyltransferases; D002208:Caproates; D004274:DNA, Recombinant; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase