[Comparison between effects of mechanical mince on the DNA damages of spleen, liver and kidney cells in mice].
OBJECTIVE
To study whether there are differences between effects of the DNA damages of the spleen, liver and kidney cells in mice caused by mechanical mince and the spleen, liver and kidney in mice can be dissociated into single cells by mechanical mince or not.


METHODS
No drug was administrated to the tested group mice, and Cyclophosphamide was administrated to the control group mice with 150 mg/kg doses. Then the three kinds of single cell suspensions were prepared by mechanical mince. The cell livabilities were detected by trypanblau coloration, and effects of mechanical mice on the DNA damages of the three kinds of cells were detected by single cell gel electrophoresis.


RESULTS
The single cell quantity prepared from spleen, liver and kidney in short time by mechanical mince can meet completely the needs of comet assay. The percentages of cells with comets of the tested group were respectively 3.20%, 6.21%, 9.22% and the DNA migrations were respectively 27.30microm, 28.45microm, 47.10microm. There were significant differences between the percentages of cells with comets in the tested groups (P < 0.05). In the DNA migration test, there were significant differences between the spleen cells and kidney cells or between liver cells and kidney cells of the three tested groups (P < 0.05). The percentages of cells with comets and the DNA migrations of the three types cells in tested groups were low significantly (P < 0.01, P < 0.001) compared with the corresponding positive control groups.


CONCLUSIONS
The effects of mechanical mince on the DNA damages of spleen, liver and kidney cells became stronger and stronger orderly. Spleen and liver can be dissociated into single cells by mechanical mince more appropriately.
Li
Xiaoyan
X
Biology Department, Medical college, Xianning College, Xianning 437100, China.
Chen
Xianjun
X
D000818:Animals; D001696:Biomechanical Phenomena; D020552:Comet Assay; D004249:DNA Damage; D007668:Kidney / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000473:pathology*