Radiolabeled choline as a proliferation marker: comparison with radiolabeled acetate.
[11C]Choline is a potential tracer to detect tumors, especially brain and prostate cancers. The metabolism of [11C]choline defines the accumulation pattern of [11C]choline in tumors depicted by positron emission tomography. Choline is a precursor of phosphatidylcholine that is a major constituent of membrane lipids. Membrane lipid synthesis as well as DNA synthesis is activated during cell proliferation. We investigated the relation between [14C]choline metabolism and proliferative activity using 10 tumor cell lines and fibroblasts. [14C]Choline uptake was higher in tumor cells than in fibroblasts and was correlated with the proliferative activity, though the sensitivity of [14C]choline uptake to proliferative activity was less than that of [1-14C]acetate. [14C]Phosphocholine produced from [14C]choline by phosphorylation mainly contributed to this accumulation. [11C]Choline can be used for the evaluation of tumor proliferation through estimating choline kinase activity.
Biomedical Imaging Research Center, University of Fukui, 23-3, Shimoaizuki, Matsuoka, Yoshida, Fukui, 910-1193, Japan.
Mitsuyoshi
M
Yoshimoto
Atsuo
A
Waki
Atsushi
A
Obata
Takako
T
Furukawa
Yoshiharu
Y
Yonekura
Yasuhisa
Y
Fujibayashi
D000085:Acetates / Q000493:pharmacokinetics*; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002794:Choline / Q000493:pharmacokinetics*; D006801:Humans; D009367:Neoplasm Staging / Q000379:methods; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic
D000085:Acetates; D014408:Biomarkers, Tumor; D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; D002794:Choline