Simultaneous determination of niflumic acid and its prodrug, talniflumate in human plasma by high performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of niflumic acid and its prodrug, talniflumate, in human plasma. Niflumic acid and talniflumate were eluted isocratically with methanol-water (73:27, v/v, adjusted to pH 3.5 by acetic acid) at a fl ow rate of 1 mL/min. Indomethacin was used as an internal standard. Signals were monitored by an UV detector at 288 nm. Retention times of indomethacin, niflumic acid and talniflumate were 5.9, 7.2 and 13.5 min, respectively. Calibration plots were linear over the range 50-5000 ng/mL for niflumic acid and 100-5000 ng/mL for talniflumate. The limits of quantitation were 50 ng/mL for niflumic acid and 100 ng/mL for talniflumate. The intra- and inter-day relative standard deviations (RSD) of niflumic acid and talniflumate were less than 10% and the accuracies were higher than 90%. This method is rapid, sensitive and reproducible for the determination of niflumic acid and talniflumate in human plasma.
College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Korea.
Dong-Jin
DJ
Jang
Jeong-Sook
JS
Park
Hye-Ran
HR
Ko
Jun-Pil
JP
Jee
Jin-Ki
JK
Kim
Sung Tae
ST
Kim
Chong-Kook
CK
Kim
D001572:Benzofurans / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D009544:Niflumic Acid / Q000097:blood* / Q000493:pharmacokinetics; D011355:Prodrugs / Q000032:analysis*; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics
D001572:Benzofurans; D011355:Prodrugs; D011725:Pyridines; D009544:Niflumic Acid; C488233:talniflumate