Melanogenesis stimulation in murine B16 melanoma cells by Piper nigrum leaf extract and its lignan constituents.
A methanolic extract from the leaves of Piper nigrum L. showed a significant stimulatory effect on melanogenesis in cultured murine B16 melanoma cells. Activity-guided fractionation of the methanolic extract led to the isolation of two known lignans, (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2), together with a new lignan, (-)-3-desmethoxycubebinin (3). Among these lignans, 1 and 2 showed a significant stimulatory activity of melanogenesis without any significant effects on cell proliferation.
Matsuda
Hideaki
H
School of Pharmaceutical Sciences, Kinki University, Higashiosaka, Osaka, Japan. matsuda@phar.kindai.ac.jp
Kawaguchi
Yoshiko
Y
Yamazaki
Miho
M
Hirata
Noriko
N
Naruto
Shunsuke
S
Asanuma
Yusuke
Y
Kaihatsu
Takayuki
T
Kubo
Michinori
M
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D051379:Mice; D029222:Piper nigrum; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves; D014407:Tumor Cells, Cultured
C501790:3,4-dimethoxy-3,4-desmethylenedioxycubebin; D017705:Lignans; D008543:Melanins; D010936:Plant Extracts; C433065:cubebin