[Effects of genistein on NOS, GSH-Px activities and NO, GSH, MDA contents in MCF human breast cancer cells].
OBJECTIVE
To investigate the mechanism that the genistein inhibit the MCF human breast cancer cell.


METHODS
The activities of glutathione peroxidase (GSH-Px), nitric oxide synthetase (NOS) and the contents of nitric oxide (NO), glutathione (GSH), malondialdehyde (MDA) both in culture supernatant and cells were examined by enzyme method and spectrophotometry respectively.


RESULTS
With genistein increasing, the activities of GSH-Px, NOS and the contents of NO increased significantly (P < 0.05), while the contents of GSH, MDA decreased both in culture supernatant and cells (P < 0.05).


CONCLUSIONS
Genistein can affect NO synthesis and antioxidation in MCF breast cancer cells. It may be one of the mechanisms that the genistein inhibit the MCF breast cancer cells growth.
School of Public Health, Shandong University, Ji'nan 250012, China.
Shu'ai
S
Sun
Xiulan
X
Zhao
Li
L
Zhao
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009569:Nitric Oxide; D008315:Malondialdehyde; D019833:Genistein; D005979:Glutathione Peroxidase; D019001:Nitric Oxide Synthase; D005978:Glutathione