Synthetic organic food colouring agents and their degraded products: effects on human and rat cholinesterases.
Most synthetic coloured additives are carcinogenic; teratogenic and cause allergic reactions. In this study, the effects of synthetic azo dyes (sunset yellow FCF and carmoisine), as well as their degraded products (sulphanilic acid and naphthionic acid), on both true and pseudo-cholinesterases (ChEs) are studied. The results indicate that the synthetic azo dyes and their degraded products inhibit both human true and pseudo-ChE activities in vitro. The concentration of coloured additive that cause 50% inhibition (IC50) and enzyme inhibitor dissociation constant (Ki) show that sunset yellow FCF produces greater inhibition of both true and pseudo-ChEs than does carmoisine and sulphanilic acid, while naphthionic acid produces greater inhibition of pseudo-ChE only. Ki indicates that the affinity of sulphanilic acid for both true and pseudo-ChEs is higher than the other three inhibitors. Inhibition of both true and pseudo-ChEs by sunset yellow FCF is of mixed (competitive and non-competitive) type, but carmoisine and sulphanilic acid are non-competitive. Naphthionic acid produces a competitive inhibition kinetic with plasma ChE only. This inhibition is abolished by dialysis, indicating that their effects are reversible. The effects of sunset yellow FCF, carmoisine, sulphanilic acid and naphthionic acid on rat true and pseudo-ChEs are investigated. The data clearly show that there is a significant decrease in enzyme activity. Sulphanilic acid and sunset yellow FCF are the most potent in vivo inhibitors of true ChE and pseudo-ChE, respectively.
Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria, Egypt. yousryosman8@hotmail.com
M Y
MY
Osman
I A
IA
Sharaf
H M Y
HM
Osman
Z A
ZA
El-Khouly
E I
EI
Ahmed
D000328:Adult; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D002802:Cholinesterases / Q000097:blood*; D003956:Dialysis; D005505:Food Coloring Agents / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009282:Naphthalenesulfonates / Q000494:pharmacology; D051381:Rats; D013425:Sulfanilic Acids / Q000494:pharmacology
D001391:Azo Compounds; D005505:Food Coloring Agents; D009282:Naphthalenesulfonates; D013425:Sulfanilic Acids; C023356:naphthionic acid; C006671:azo rubin S; D002802:Cholinesterases; C005842:6-hydroxy-5-((p- sulfophenyl)azo)-2-naphthalenesulfonic acid disodium salt