Neurophysiological and immunohistochemical studies on Guillain-Barre syndrome with IgG anti-GalNAc-GD1a antibodies-effects on neuromuscular transmission.
We investigated the epitopes and functional role of IgG anti-GalNAc-GD1a antibodies appearing in serum from a patient with Guillain-Barre syndrome (GBS) and IgG anti-GalNAc-GD1a antibody that was produced by immunization of a rabbit with GalNAc-GD1a. Both sera blocked neuromuscular transmission in muscle-spinal cord co-culture cells. The acetylcholine-induced potential did not reduce by adding sera, suggesting that the blockade is presynaptic. The effect was complement-independent and purified IgG from serum of the patient or the rabbit had the same effects. The epitope with both anti-GalNAc-GD1a antibodies was observed in the soma of large neurons in the anterior horns of the adult rat spinal cord and their motor axons of rat ventral roots. Both anti-GalNAc GD1a antibodies reacted strongly with the motor nerve terminals in rats. The anti-GalNAc-GD1a antibodies may block neuromuscular transmission by attacking on presynaptic motor axon, probably affecting the ion channels in the presynaptic motor axon.
Department of Neuroscience, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, Tokyo 194-8543, Japan.
Kyoji
K
Taguchi
Jin
J
Ren
Iku
I
Utsunomiya
Hiroyuki
H
Aoyagi
Nobuya
N
Fujita
Toshio
T
Ariga
Tadashi
T
Miyatake
Hiide
H
Yoshino
D000109:Acetylcholine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000368:Aged; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000494:pharmacology; D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods; D002038:Bungarotoxins / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D003714:Denervation / Q000379:methods; D004594:Electrophysiology / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005732:Gangliosides / Q000276:immunology* / Q000378:metabolism; D020275:Guillain-Barre Syndrome / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D009469:Neuromuscular Junction / Q000378:metabolism; D009924:Organ Culture Techniques; D011817:Rabbits; D051381:Rats; D013116:Spinal Cord / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects*
D000906:Antibodies; D002038:Bungarotoxins; D005732:Gangliosides; D007074:Immunoglobulin G; C055253:IV(4)-galactosyl-N-acetylganglioside GD1a; D000109:Acetylcholine