Green fluorescent protein as a marker for monitoring a pentachlorophenol degrader Sphingomonas chlorophenolica ATCC39723.
Sphingomonas chlorophenolica ATCC39723 was successfully labeled with the gfp (green fluorescent protein) gene inserted into the pcpB gene by homologous recombination. As the gfp recombinant was easily distinguished from other indigenous organisms, the population of gfp recombinant was monitored after being released into the soil microcosms. Their population density dropped from 10(8) to 10(6) (cfu/ml) in the non-sterilized soil microcosms during the first 6 days. Moreover, the gfp recombinant was not detected even at lower dilution rates after a certain time period. The recombinant, however, survived for at least 28 days in the sterilized soil microcosms. Although the gfp recombinant did not degrade pentachlorophenol (PCP), this experiment showed the possibility of using gfp as a monitoring reporter system for S. chlorophenolica ATCC39723 and potentially other species of Sphingomonas.
Department of Biological Engineering, Center for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea.
Eun-Taex
ET
Oh
Jae-Seong
JS
So
Byung-Hyuk
BH
Kim
Jong-Sul
JS
Kim
Sung-Cheol
SC
Koh
D001673:Biodegradation, Environmental; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism*; D010416:Pentachlorophenol / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants; D020578:Sphingomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D005819:Genetic Markers; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D012989:Soil Pollutants; D049452:Green Fluorescent Proteins; D010416:Pentachlorophenol