Synthesis of nanosized chitosan-poly(acrylic acid) particles by a dropping method.
Deoxyribonucleic acid (DNA) vaccines are being investigated extensively because of their excellent potential over conventional protein ones. A suitable DNA carrier, consisting of uniformly dispersed chitosan-poly(acrylic acid) particles with an average size of 30 nm, was successfully synthesized by a dropping method with a ratio of chitosan solution to poly(acrylic acid) solution of 1:1 and was incubated in a buffer solution with a pH value of 3.0. The particle size increased from 35.76 to 45.90 nm when the pH value of the buffer solution was increased from 3.0 to 7.4. After freeze-drying, the non-incubated mixed solution showed a membranous morphology. A powdered product was formed from the mixed solution as incubated in buffer solution with pH values of 3.0 and 5.3. However, when the mixed solution was incubated in a buffer solution of pH 7.4, a mixture of membrane and powder was obtained.
Chang
Tao-Chih
TC
Department of Materials Science and Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan.
Wang
Jian-Wen
JW
Hon
Min-Hsiung
MH
D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning; D014961:X-Ray Diffraction
D000180:Acrylic Resins; D007202:Indicators and Reagents; C065246:chitosan-poly(acrylic acid); D048271:Chitosan