Indirect covariance NMR spectroscopy.
A novel NMR scheme is presented that establishes homonuclear spin correlations without requiring direct detection of the spin species. This covariance NMR method is experimentally demonstrated for a mixture of amino acids and for the uniformly 13C-labeled cyclic decapeptide antamanide using a 13C-edited TOCSY experiment. The method opens up new avenues for the experimental analysis of molecules containing insensitive spins encountered in biomolecular NMR and analytical chemistry including metabolomics.
Zhang
Fengli
F
Carlson School of Chemistry and Biochemistry, Clark University, Worcester, Massachusetts 01610, USA.
Brüschweiler
Rafael
R
D005583:Fourier Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010456:Peptides, Cyclic / Q000737:chemistry
D010456:Peptides, Cyclic; C100265:antamanide