Expression of bone morphogenetic proteins, receptors, and tissue inhibitors in human fetal, adult, and osteoarthritic articular cartilage.
Coordinate expression of BMPs and their receptors and inhibitors is likely necessary for physiologic BMP regulation and activity. To characterize the expression of such factors in fetal, normal adult, and end-stage osteoarthritic articular cartilage, samples from these sources were analyzed. PCR-amplified sequences (BMPs 1-11), receptors (IA, IB, II), TGF-beta1, TGF-beta2, inhibitors noggin and follistatin, CDMP-1, COMP, and GAPDH from cDNAs generated from extracted total RNA were resolved by gel electrophoresis. Protein levels of BMPs 3, 7, and 8 were also analyzed by SDS-PAGE and Western blotting. RT-PCR revealed that BMPs 1, 2, 4-6, and 11, BMPR-IA and II, noggin, follistatin, CDMP-1, COMP, and GAPDH mRNAs were expressed in similar fashion in both fetal and adult (normal or osteoarthritic) cartilage. BMPs 9 and 10 mRNAs were not expressed in either group. BMPs 7, 8, and BMPR-IB mRNAs were consistently expressed in fetal but not in adult cartilage. BMP-3 mRNA was expressed in fetal and normal adult, but not in osteoarthritic samples. TGF-beta1 was expressed in both adult normal and osteoarthritic, but not fetal, samples. Similarly, Western blotting demonstrated BMPs 7 and 8 to be present in fetal but not in adult samples. BMP-3 protein was present in fetal and adult normal samples, to a lesser extent, but absent in osteoarthritic cartilage.
Department of Orthopaedic Surgery, Musculoskeletal Research Center, NYU-Hospital for Joint Diseases Orthopaedic Institute, 301 East 17th Street, New York, NY 1003, USA.
Andrew L
AL
Chen
Carrie
C
Fang
Chuanju
C
Liu
Michael P
MP
Leslie
Eric
E
Chang
Paul E
PE
Di Cesare
D000368:Aged; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002352:Carrier Proteins; D064236:Cartilage Oligomeric Matrix Protein; D002358:Cartilage, Articular / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005333:Fetus; D038681:Follistatin / Q000235:genetics; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000235:genetics; D055428:Growth Differentiation Factor 5; D006131:Growth Inhibitors / Q000235:genetics*; D006801:Humans; D064235:Matrilin Proteins; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics / Q000503:physiopathology*; D011506:Proteins / Q000235:genetics; D017978:Receptors, Growth Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099176:Noggin Protein
D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D038681:Follistatin; D006023:Glycoproteins; D055428:Growth Differentiation Factor 5; D006131:Growth Inhibitors; D064235:Matrilin Proteins; D011506:Proteins; D017978:Receptors, Growth Factor; D000099176:Noggin Protein; C577553:TSP5 protein, human