Inhibition of HIV-1 infection in cells expressing an artificial complementary peptide.
TLMA2993 peptide (N'-TLMALELKGKLLLAGLAPSAFLPLSFPEGL-C') which was designed by a computer program (MIMETIC) inhibited the activity of HIV-1 reverse transcriptase in a cell-free system. Therefore, we constructed a TLMA2993 expression vector containing an artificial cDNA for TLMA2993 to generate the peptide in cells. The cell lysate of transfected U937 cells contained a detectable level of TLMA2993 peptide using competitive ELISA. The transfectants were resistant to HIV-1 infection due to expression of TLMA2993 peptide in the cells. The use of MIMETIC to design an inhibitory peptide to any intracellular target molecule, followed by transfection of the artificial cDNA for the peptide, could afford a new approach for treatment and/or prevention of viral infection.
Hosokawa
Masato
M
Department of Biodefence, Nagoya City University Graduate School of Medical Science, 1 Kawasumi, Mizuho-cho, Nagoya 467-8601, Japan.
Imai
Masaki
M
Okada
Hidechika
H
Okada
Noriko
N
D002460:Cell Line; D015195:Drug Design; D005822:Genetic Vectors; D015658:HIV Infections / Q000378:metabolism*; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000378:metabolism*; D012984:Software
D010455:Peptides; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase