Genetic variability and taxonomic position of ectomycorrhizal fungus Pisolithus from India.
Eight ectomycorrhizal fungal isolates of Pisolithus associated with Eucalyptus species in different parts of India were collected and the genetic variability of these isolates was studied by ITS-RFLP and ITS sequencing. All the isolates showed same RFLP patterns with each restriction enzyme, indicating all these isolates of Pisolithus are of the same genotype. The sequence comparison of KN6 of Indian isolate showed high sequence similarities with the isolates of Pisolithus associated with Eucalyptus from Australia. Phylogeny analysis showed that all the isolates compared in this study clustered into four main groups The Indian isolate (KN6) clustered with Pisolithus albus isolates of group I, which are associated with Eucalyptus. These results suggested that Pisolithus isolates found in India are P. albus.
Singla
Shaveta
S
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147 004, India.
Reddy
M S
MS
Marmeisse
R
R
Gay
G
G
D001487:Basidiomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005052:Eucalyptus / Q000382:microbiology*; D014644:Genetic Variation; D007194:India; D016533:Mycological Typing Techniques; D038821:Mycorrhizae; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer