Interaction between insulin-like growth factor-1 with other growth factors in serum depleted culture medium for human cartilage engineering.
The regulation roles of insulin-like growth factor-1 (IGF-1) with basic fibroblast growth factor (bFGF) and transforming growth factor beta 2 (TGFbeta2) in human nasal septum chondrocytes monolayer culture and cartilage engineering was investigated in this study. The role of IGF-1 with bFGF and TGFbeta2 was investigated by measuring chondrocyte growth kinetic and collagen genes expression. IGF-1 together with bFGF and TGFbeta2 promote cartilage tissue engineering, increase type II collagen expression and enhance the histological features of engineered cartilage.
Chua
K H
KH
Department of Physiology, Medical Faculty of Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Fuzina
N H
NH
Ruszymah
B H I
BH
D002356:Cartilage / Q000637:transplantation*; D002455:Cell Division / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology*; D024043:Collagen Type II / Q000235:genetics; D016895:Culture Media, Serum-Free; D015870:Gene Expression / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D024043:Collagen Type II; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D007334:Insulin-Like Growth Factor I