Cloning and expression of an SH3 domain-containing protein (Xchef-1), a novel downstream target of activin/nodal signaling.
The activity of the activin/nodal signaling cascade is essential for the proper specification of germ layers during gastrulation. Many of the components of this signaling pathway have been identified, but relatively few downstream targets have been discovered. Using cDNA microarrays, we have identified a novel SH3-domain-containing gene we have named Xchef-1 that is upregulated in response to activin/nodal signaling. Xchef-1 is a direct downstream target of activin and is expressed in the marginal zones of gastrulating Xenopus embryos in a dynamic pattern reminiscent of nodal expression. At neurula stages, Xchef-1 is expressed in neural crest of the head and trunk as well as in the anterior neural plate. These domains of expression are then restricted at tailbud stages to the branchial arches, and the region of the future gall bladder.
Meek
Lauren M
LM
Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697-2300, USA. lmeek@uci.edu
Hayata
Tadayoshi
T
Shin
Yong-Chol
YC
Evinger
Albert J
AJ
Cho
Ken W Y
KW
D029404:Activin Receptors / Q000378:metabolism*; D028341:Activins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006389:Hemagglutinins, Viral / Q000737:chemistry*; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000196:embryology*; D055457:Nodal Protein; D020411:Oligonucleotide Array Sequence Analysis; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000737:chemistry*; D014981:Xenopus; D029867:Xenopus Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D018909:src Homology Domains
C498339:CASS4 protein, Xenopus; D018076:DNA, Complementary; D006389:Hemagglutinins, Viral; D047908:Intracellular Signaling Peptides and Proteins; D055457:Nodal Protein; D016212:Transforming Growth Factor beta; D014760:Viral Fusion Proteins; D029867:Xenopus Proteins; C060814:hemagglutinin esterase; D028341:Activins; D012313:RNA; D029404:Activin Receptors