Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures.
Studies on CFTR protein expression and localization in native tissues or in primary cultures of human epithelial cells are scarce due to the intrinsic instability of this protein, its low expression in most tissues and also to technical difficulties. However, such data are of the highest importance to understand the pathophysiology of CF. The purpose of this article is to outline several assays for the characterization of primary epithelial cultures and to review different CFTR immunostaining protocols.
Mendes
Filipa
F
Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Av Padre Cruz, 1649-016, Lisboa, Portugal. filipa.mendes@insa.min-saude.pt
Doucet
Laurent
L
Hinzpeter
Alexandre
A
Férec
Claude
C
Lipecka
Joanna
J
Fritsch
Janine
J
Edelman
Aleksander
A
Jorna
Huub
H
Willemsen
Rob
R
Bot
Alice G M
AG
De Jonge
Hugo R
HR
Hinnrasky
Jocelyne
J
Castillon
Nicolas
N
Taouil
Karima
K
Puchelle
Edith
E
Penque
Deborah
D
Amaral
Margarida D
MD
D018929:Cell Culture Techniques; D003550:Cystic Fibrosis / Q000235:genetics / Q000276:immunology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000276:immunology*; D004847:Epithelial Cells; D015870:Gene Expression / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator