Isolation and transcriptional regulation of the Kluyveromyces lactis FBA1 (fructose-1,6-bisphosphate aldolase) gene.
Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is mediated by heme since expression was higher in a mutant defective in heme biosynthesis. KlFBA1 transcription is not induced by calcium-shortage, low temperature, or at stationary phase. These data suggest that KlFBA1 plays a role in the balance between oxidative and fermentative metabolism and that this gene is differentially regulated in K. lactis and Saccharomyces cerevisiae, i.e., a respiratory vs. fermentative yeast.
Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira s/n, 15071- A Coruña, Spain.
Silvia M Díaz
SM
Prado
M Esperanza
ME
Cerdán
M Isabel
MI
González Siso
D003001:Cloning, Molecular; D003470:Culture Media; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006418:Heme / Q000494:pharmacology; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D010100:Oxygen / Q000494:pharmacology; D014158:Transcription, Genetic
D003470:Culture Media; D005656:Fungal Proteins; D006418:Heme; D005634:Fructose-Bisphosphate Aldolase; D010100:Oxygen