A strategy for functional proteomic analysis of glycosidase activity from cell lysates.
Vocadlo
David J
DJ
Center for New Direction in Organic Synthesis, Howard Hughes Medical Institute, Departments of Chemistry and Molecular and Cell Biology, University of California, Room 419, Latimer Hall, Berkeley, CA 94720-1460, USA. dvocadlo@sfu.ca
Bertozzi
Carolyn R
CR
D000818:Animals; D002457:Cell Extracts / Q000032:analysis*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
D002457:Cell Extracts; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase