Use of a duplex-PCR assay to screen for Feline Herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats.
Fifty-four ocular and forty-six pharyngeal swabs, collected from 54 cats with respiratory syndrome, were analyzed by duplex-PCR to evaluate the presence of Feline Herpesvirus type 1 and Chlamydophila spp. Both pathogens are in the population of cats and as four cats were positive only in ocular swabs and three only in pharyngeal ones, it is deduced that a correct diagnostic approach has to foresee the dispatch to the laboratory of both swabs. Furthermore, all chlamydophila strains analysed by endonuclease restriction were classified as Chlamydophila felis.
Department of Scienze Biomediche Comparate, Faculty of Veterinary Medicine, University of Teramo, Italy.
F
F
Marsilio
B
B
Di Martino
C
C
Di Francesco
D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D023501:Chlamydophila / Q000145:classification / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000175:diagnosis / Q000662:veterinary*; D005123:Eye / Q000382:microbiology / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D010614:Pharynx / Q000382:microbiology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018140:Varicellovirus / Q000145:classification / Q000302:isolation & purification*