Large-scale carbohydrate analysis by capillary array electrophoresis: part 1. Separation and scale-up.
A 96-capillary array electrophoresis (CAE) instrument has been adapted for large-scale mono- and oligosaccharide analysis and characterization. Operational protocols and data processing tools have been developed to optimize the CAE system for this application. Effects of different additives to the running buffer on efficiency and capillary-to-capillary performance reproducibility have been studied.
Diversa Corporation, San Diego, CA 92121, USA. jkhandurina@diversa.com
Julia
J
Khandurina
Nels A
NA
Olson
Abraham A
AA
Anderson
Kevin A
KA
Gray
Andras
A
Guttman
D002021:Buffers; D002241:Carbohydrates / Q000032:analysis* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results
D002021:Buffers; D002241:Carbohydrates