[Effect of human acellular amnion membrane on tendon adhesion in rat].
OBJECTIVE
To investigate the effects of human acellular amnion membrane on SD rat tendon adhesion and to obtain the experimental data for clinical application in preventing postoperative tendon adhesion.


METHODS
The tendons of 28 adult SD rats hindlimb were cut and sutured. The tendons of left hindlimb were encapsulated by human accellular amnion membrane as the experimental group and the ones of the other side were not encapsulated as control group. The rats were killed 1, 2, 4, 6, 8 and 12 weeks after operation. The results were evaluated grossly and histologically.


RESULTS
There were no differences in healing of injury tendon and inflammatory response between the two groups. The anatomical and histological results showed the experimental group had less adhesion than the control group (P < 0.05).


CONCLUSIONS
Human acellular amnion membrane can prevent adhesion of tendon without affecting tendon healing and is an optimal biological material to prevent tendon adhesion.
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases of Ministry of Education, West China Hospital, Sichuan University, Chengdu Sichuan 610041, PR China. orthop@public.cd.sc.cn
Jingcong
J
Luo
Zhiming
Z
Yang
Xiuqun
X
Li
D000650:Amnion / Q000637:transplantation*; D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D011183:Postoperative Complications / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D013708:Tendon Injuries / Q000601:surgery*; D013710:Tendons / Q000601:surgery*; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control; D014183:Transplantation, Heterologous; D014945:Wound Healing / Q000187:drug effects
D008567:Membranes, Artificial