Factors inducing in-stent restenosis: an in-vitro model.
In-stent restenosis is caused by the proliferation of the smooth muscle cells (SMCs) following a host response towards the implanted device. However, the precise biochemical and cellular mechanisms are still not completely understood. In this paper, the behaviour of SMCs has been investigated by an in vitro model where the cells were stimulated by platelet derived growth factor (PDGF) on tissue-like substrates as well as on biomaterials such as stainless steel (St) and diamond-like carbon (DLC)-coated St. The results demonstrated that SMCs have a completely different adhesion mode on St and become particularly prone to proliferation and pro-inflammatory cytokine secretion under PDGF stimulus. This would suggest that restenosis may caused by the accidental contact of the SMC with the St substrate under an inflammatory insult.
School of Pharmacy & Biomolecular Sciences, University of Brighton, Cockcroft Building Lewes Road, Brighton, UK.
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M
Santin
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C
Morris
M
M
Harrison
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L
Mikhalovska
A W
AW
Lloyd
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Mikhalovsky
D002244:Carbon; D000077482:Carbon Fiber; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D023903:Coronary Restenosis / Q000503:physiopathology*; D016207:Cytokines; D046169:Dermoscopy; D006801:Humans; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology*; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D011474:Prosthesis Design; D013193:Stainless Steel; D015607:Stents
D000077482:Carbon Fiber; D020099:Coated Materials, Biocompatible; D016207:Cytokines; D010982:Platelet-Derived Growth Factor; D013193:Stainless Steel; D002244:Carbon