Generation of hybrid cell lines with endothelial potential from spontaneous fusion of adult bone marrow cells with embryonic fibroblast feeder.
We have previously isolated mouse embryonic cell lines with endothelial potential using a simple empirical approach. In an attempt to isolate similar cell lines from adult mouse bone marrow (BM), BM cells were cultured on mitotically inactive mouse embryonic fibroblast (MEF) feeder cells. Several cell lines with putative endothelial potential were generated. They expressed endothelial-specific genes and formed vascular-like structures when plated on matrigel. When transplanted into appropriate mouse models, they incorporated into the endothelium of the vasculature. Similar cell lines were also obtained using human or porcine BM. None of these lines induced tumor formation when transplanted into immunodeficient Rag1-/- mice. However, all the lines were aneuploid with genetic markers from BM samples and the MEF feeder, suggesting that they resulted from a non-species-specific fusion of a BM cell and mitotically inactive MEF. Together, these lines demonstrated for the first time that BM cells can also undergo fusion with commonly used mitotically inactive feeder cells. Although these fusion cell lines were culture artifacts, their derivation would be useful in understanding fusion of BM cells with other cell types, and their endothelial potential will also be useful in characterizing endothelial differentiation.
National University Medical Institute, National University of Singapore, Singapore.
Jianwen
J
Que
Reida Menshawe
RM
El Oakley
Manuel
M
Salto-Tellez
Nathalie
N
Wong
Dominique P V
DP
de Kleijn
Ming
M
Teh
Leslie
L
Retnam
Sai-Kiang
SK
Lim
D000782:Aneuploidy; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D002459:Cell Fusion; D002460:Cell Line; D002461:Cell Line, Transformed / Q000166:cytology / Q000378:metabolism; D003094:Collagen; D004338:Drug Combinations; D004622:Embryo, Mammalian / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D007797:Laminin; D008099:Liver / Q000098:blood supply; D051379:Mice; D008810:Mice, Inbred C57BL; D016685:Mitomycin / Q000494:pharmacology; D009203:Myocardial Infarction; D018919:Neovascularization, Physiologic / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011509:Proteoglycans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013552:Swine
D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D016685:Mitomycin; D003094:Collagen