Modulation of matrix metalloproteinase-9 activity by hyaluronan is dependent on NF-kappaB activity in lymphoma cell lines with dissimilar invasive behavior.
Expression and activity of matrix metalloproteinase-9 (MMP-9) as well as its relationship with hyaluronan (HA) and NF-kappaB activity were analyzed in two murine lymphoma cell lines with dissimilar migration and invasive behavior. MMP activity was evaluated by zymograms in supernatants, membrane extracts of tumor cells, and in the organs invaded by these cells. The more aggressive LBLa cell line showed MMP-9 activity in vitro, which increased after HA treatment and was blocked by anti-CD44 mAb. Such activity was not found in the less aggressive LBLc. MMP-9 and MMP-2 activity was found in organs invaded by both cell lines, although differential MMP-9 activity was observed in lung infiltrated only by LBLa cell line. NF-kappaB activation was evaluated to determine whether differential activity of MMP-9 was dependent on downstream signaling pathway, showing higher NF-kappaB activity in the more aggressive LBLa cell line. Our results showed that MMP-9 activity modulated by HA through NF-kappaB signaling pathway may be involved in the aggressive behavior of LBLa.
Cátedra de Inmunología-IDEHU, Facultad de Farmacia y Bioquímica, UBA-CONICET, Junín 956, piso 4, CP: 1113, Buenos Aires, Argentina. laulaniz@ffyb.uba.ar
Laura
L
Alaniz
Mariana
M
García
Paula
P
Cabrera
María
M
Arnaiz
Victoria
V
Cavaliere
Guillermo
G
Blanco
Elida
E
Alvarez
Silvia
S
Hajos
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008223:Lymphoma / Q000378:metabolism*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D018076:DNA, Complementary; D018960:Hyaluronan Receptors; D016328:NF-kappa B; D011994:Recombinant Proteins; D006820:Hyaluronic Acid; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9