[Alfa-subunit composition of neuronal nicotinic acetylcholine receptors of the submucosal plexus in the guinea-pig small intestine].
The alpha-subunit composition of nicotinic acetylcholine receptors (nAChRs) in the submucosal plexus of the guinea-pig ileum were studied using the whole-cell patch-clamp technique and affinity-purified antibodies (Abs) against alpha3-, alpha4, alpha5 and alpha7-subunits of nAChRs. The independent addition of anti-alpha3 and anti-alpha5 Abs to extracellular bath solution induced depression of the acetylcholine (ACh)-evoked currents by 60% +/- 1.56% and 65% +/- 1.62% correspondingly. Successive application of anti-alpha5 Abs in the presence of anti-alpha3 Abs did not have any additional blocking effect on ACh-evoked currents. Anti-alpha7 Abs evoked depression of Ach-induced currents by 24% +/- 1.51% in 80% of investigated neurons and by 67% +/- 1.5% in 20% of neurons. The addition of anti-alpha4 Abs to extracellular bath solution did not have effect on membrane currents of the investigated neurons. Our data provide evidence of participation of alpha3-, alpha5 and alpha7-containing receptors in the response to ACh. Alpha3- and alpha5-subunits are associated in the same functional nAChRs that provide greater part of response to ACh in the most of submucosal neurons. However, in some neurones alpha7-containing nAChRs provide greater part of response to agonist.
Hryhorov
O O
OO
Skok
M V
MV
Skok
V I
VI
D000109:Acetylcholine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000294:innervation*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013368:Submucous Plexus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D021122:Protein Subunits; D011978:Receptors, Nicotinic; D000109:Acetylcholine