Antiapoptotic effects of budipine.
Apoptosis is one essential step for neuronal death in the nigrostriatal region in patients with Parkinson's disease. Cytotoxic tumor necrosis factor-alpha (TNF-alpha) and the proinflammatory cytokine interleukin-6 (Il-6) provide a proapoptotic environment. We investigated the influence of the antiparkinsonian compound budipine on the release of TNF-alpha and Il-6 in peripheral blood mononuclear cells (PBMC) and on the degree of cisplatin induced apoptotic cell death in SH-SY 5Y human neuroblastoma cells. 10(-7), 10(-8), 10(-9) mol/l of budipine significantly reduced release of TNF-alpha and Il-6 in PBMC and decreased apoptotic cell death after 50 hours and 74 hours in the SH-SY 5Y cells. Our results suggest, that budipine administration provides an antiapoptotic environment and slows neuronal apoptotic and inflammatory mediated loss of neurons.
Department of Neurology, St. Josef-Hospital, University of Bochum, Bochum, Germany. thomas.mueller@ruhr-uni-bochum.de
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Müller
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Przuntek
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Krüger
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Mackowiak
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000978:Antiparkinson Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D010880:Piperidines / Q000494:pharmacology*; D013564:Sympathetic Nervous System / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000978:Antiparkinson Agents; D015850:Interleukin-6; D010880:Piperidines; D014409:Tumor Necrosis Factor-alpha; C026710:budipine