Mechanically engineered hydrogel scaffolds for axonal growth and angiogenesis after transplantation in spinal cord injury.
OBJECTIVE
Spinal cord injury (SCI) is a complex pathological entity, the treatment of which requires a multipronged approach. One way to integrate different therapeutic strategies for SCI is to develop implantable scaffolds that can deliver therapies in a synergistic manner. Many investigators have developed implantable "bridges," but an important property of such scaffolds--that is, mechanical compatibility with host tissues--has been neglected. In this study, the authors evaluated the results of implanting a mechanically matched hydrogel-based scaffold to treat SCI.


METHODS
A nonbiodegradable hydrogel, poly(2-hydroxyethylmethacrylate) (PHEMA), was engineered using thermally initiated free radical solution polymerization. Two groups of 12 adult Sprague-Dawley rats underwent partial cervical hemisection injury followed by implantation of either PHEMA or PHEMA soaked in 1 microg of brain-derived neurotrophic factor (BDNF). Four rats from each group were killed 1, 2, or 4 weeks after induction of the injury. Immunofluorescence staining was performed to determine the presence of scarring, cellular inflammatory responses, gliosis, angiogenesis, and axonal growth in and around the implanted scaffolds.


CONCLUSIONS
The implanted PHEMA with 85% water content had a compressive modulus of 3 to 4 kPa, which matched the spinal cord. Implanted PHEMA elicited modest cellular inflammatory responses that disappeared by 4 weeks and minimal scarring was noted around the matrix. Considerable angiogenesis was observed in PHEMA, and PHEMA soaked in BDNF promoted axonal penetration into the gel. The authors conclude that mechanically engineered PHEMA is well accepted by host tissues and might be used as a platform for sustained drug delivery to promote axonal growth and functional recovery after SCI.
Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA. ab99@drexel.edu
Ajay
A
Bakshi
Omar
O
Fisher
Taner
T
Dagci
B Timothy
BT
Himes
Itzhak
I
Fischer
Anthony
A
Lowman
D000818:Animals; D001369:Axons / Q000502:physiology*; D020099:Coated Materials, Biocompatible; D004343:Drug Implants; D020100:Hydrogels; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects*; D011102:Polyhydroxyethyl Methacrylate; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology
D020099:Coated Materials, Biocompatible; D004343:Drug Implants; D020100:Hydrogels; D011102:Polyhydroxyethyl Methacrylate