[A preliminary study on gene expression profile induced by water stress in wheat (Triticum aestivum L.) seedling].
In the present research, suppression subtractive hybridization (SSH) and high density membrane techniques were employed to analysis genes induced by water stress in wheat seedling at 2-leaf stage. The purpose was to comprehensively understand the genetic bases of drought resistance and to find the key genes related to drought resistance in wheat. A total of 181 positive clones were obtained by screening the SSH library including 1 530 individual recombinant clones. The result of the sequence homologous comparison and function querying showed that 83.2% differentially expressed genes were high homologous with those induced by abiotic or biotic stresses in plant and their functions were to protect the cell directly or indirectly from stress strain. Seventeen differentially expressed ESTs found first were registered in GenBank. After further identifying by reverse Northern, RT-PCR and Northern, the gene expression profile induced by water stress in wheat seedling was preliminarily revealed.
Wang
Zhuan
Z
Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Zang
Qing-Wei
QW
Guo
Zhi-Ai
ZA
Jing
Rui-Lian
RL
D015152:Blotting, Northern; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics*; D014908:Triticum / Q000235:genetics*; D014867:Water
D014867:Water