[Positional analysis of a gene related to salt tolerance in Sinorhizobium meliloti by transposon rescue].
Salt sensitive mutant 042BML-2 was obtained by mutating Sinorhizobium meliloti 042BM with transposon Tn5 carried on the plasmid pRL1063a. By transposon rescue, a 1.179 kb of DNA sequence of S. meliloti flanking the Tn5 insertion site was obtained. The sequence similarity analysis through BLAST analysis in GenBank revealed the transposon was inserted into a functionally unknown gene, which is 6 270 bp in length, of S. meliloti, and the gene was named rtsC. This study indicated that rtsC was associated with salt tolerance in S. meliloti 042BM. Hydrophobicity profile analysis of the putative amino acid sequence showed that two predicted transmembrane domains existed in N-terminal portion of RtsC. The significance of RtsC protein in the salt-tolerance in S. meliloti was discussed.
Li
Xiao-Hong
XH
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Microbial Resource and Application of Agricultural Ministry, Beijing 100094, China.
Du
Bing-Hai
BH
Zhang
Xiao-Qing
XQ
Wang
Lei
L
Yang
Su-Sheng
SS
D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016962:Sinorhizobium meliloti / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*
D004251:DNA Transposable Elements; D012965:Sodium Chloride