Cloning and sequencing analysis of the repressor gene of temperate mycobacteriophage L1.
The wild-type and temperature-sensitive (ts) repressor genes were cloned from the temperate mycobacteriophage L1 and its mutant L1cIts391, respectively. A sequencing analysis revealed that the 131st proline residue of the wild-type repressor was changed to leucine in the ts mutant repressor. The 100% identity that was discovered between the two DNA regions of phages L1 and L5, carrying the same sets of genes including their repressor genes, strengthened the speculation that L1 is a minor variant of phage L5 or vice versa. A comparative analysis of the repressor proteins of different mycobacteriophages suggests that the mycobacteriophage-specific repressor proteins constitute a new family of repressors, which were possibly evolved from a common ancestor. Alignment of the mycobacteriophage-specific repressor proteins showed at least 7 blocks (designated I-VII) that carried 3-8 identical amino acid residues. The amino acid residues of blocks V, VI, and some residues downstream to block VI are crucial for the function of the L1 (or L5) repressor. Blocks I and II possibly form the turn and helix 2 regions of the HTH motif of the repressor. Block IV in the L1 repressor is part of the most charged region encompassing amino acid residues 72-92, which flanks the putative N-terminal basic (residues 1-71) and C-terminal acidic (residues 93-183) domains of L1 repressor.
Department of Biochemistry, Bose Institute, P1/12-CIT Scheme VII M, Calcutta 700 054, India. sau@bic.boseinst.ernet.in
Subrata
S
Sau
Partho
P
Chattoraj
Tridib
T
Ganguly
Chia Yen
CY
Lee
Nitai Chandra
NC
Mandal
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics; D019143:Evolution, Molecular; D019077:Helix-Turn-Helix Motifs / Q000235:genetics; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D007930:Leucine / Q000378:metabolism; D008242:Lysogeny / Q000235:genetics*; D008969:Molecular Sequence Data; D009160:Mycobacteriophages / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004279:DNA, Viral; D012097:Repressor Proteins; D007930:Leucine