Chromatic immunoassay based on polydiacetylene vesicles.
A new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 10,12-tricosadiynoic acid (TCDA) and dimyristoylphosphatidycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay.
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
Yan-Lei
YL
Su
Jin-Ru
JR
Li
Long
L
Jiang
D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry; D003124:Colorimetry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes; D012680:Sensitivity and Specificity; D013499:Surface Properties
C434762:10,12-tricosadiynoic acid; D000906:Antibodies; D005231:Fatty Acids, Unsaturated; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D000114:Acetylene; D004134:Dimyristoylphosphatidylcholine