N-acetylcysteine inhibits peroxynitrite-mediated damage in oleic acid-induced lung injury.
Since oleic acid (OA) induces morphologic and cellular changes similar to those observed in human acute lung injury (ALI) and acute respiratory distress syndrome, it has become a widely used model to investigate the effects of several agents on pathogenesis of lung injury. The antioxidant and anti-inflammatory properties of N-acetylcysteine (NAC) has been documented in many lung injury models. In this study, we evaluated the role of NAC in an OA-induced lung injury model by measuring myeloperoxidase (MPO) activity, malondialdehyde (MDA) and 3-nitrotyrosine (3-NT) levels in lung tissue. Five groups labelled Sham, NAC, OA, Pre-OA-NAC and Post-OA-NAC were determined. ALI was induced by intravenous administration of OA. The pre-OA-NAC group received iv NAC 15 min before OA infusion and the post-OA-NAC group received iv NAC 2 h after OA infusion. In both of the NAC treatment groups' blood and tissue samples were collected 4 h after OA infusion, independent from the time of NAC infusion. The MPO activity, MDA and 3-NT levels in lung homogenates were found to be increased in OA group and the administration of NAC significantly reduced tissue MPO, MDA and 3-NT levels (p = 0.0001) Lung histopathology was also affected by NAC in this OA-induced experimental lung injury model.
Department of Thoracic Surgery, School of Medicine, Mersin University, Mersin Universitesi Tip Fakültesi Hastanesi, Zeytinlibahçe Caddesi 33079, Mersin, Turkey. oguzkoksel@mersin.edu.tr
Oguz
O
Koksel
Ismail
I
Cinel
Lulufer
L
Tamer
Leyla
L
Cinel
Ali
A
Ozdulger
Arzu
A
Kanik
Bahadir
B
Ercan
Ugur
U
Oral
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D005260:Female; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D008853:Microscopy; D019301:Oleic Acid / Q000009:adverse effects*; D009195:Peroxidase / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D030421:Peroxynitrous Acid; D019301:Oleic Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D008315:Malondialdehyde; D009195:Peroxidase; D000111:Acetylcysteine