Increased stability of bmr3 mRNA results in a multidrug-resistant phenotype in Bacillus subtilis.
A spontaneous mutant isolated in the presence of a high concentration of puromycin acquired a multidrug-resistant phenotype. Expression of the bmr3 gene was dramatically increased. A base substitution, T to A at the +4 position, detected in the mutant resulted in the stabilization of bmr3 mRNA.
Department of Molecular Biology, School of Health Sciences, Kyorin University, 476 Miyashita, Hachioji, Tokyo 192-0005, Japan. ohkir@kyorin-u.ac.jp
Reiko
R
Ohki
Kozue
K
Tateno
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011691:Puromycin / Q000494:pharmacology; D020871:RNA Stability; D012329:RNA, Bacterial; D012333:RNA, Messenger
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C104245:Bmr3 protein, Bacillus subtilis; D026901:Membrane Transport Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011691:Puromycin