4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH.
Thymocytes exposed to the pro-oxidant tert-butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane-bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.
Department of Biochemistry, Medical University, Gdańsk, Poland.
Ewa
E
Kossowka
Agata
A
Zauszkiewicz
Jolanta
J
Kubasik-Juraniec
Cecylia
C
Tukaj
Jan Henry
JH
Spodnik
Anna
A
Hallman
Jerzy
J
Klimek
Emilia
E
Syta
Adam
A
Figarski
Takashi
T
Wakabayashi
Michał
M
Woźniak
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D048430:Cell Shape; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000378:metabolism*
D000975:Antioxidants; D003497:Cyclic N-Oxides; D020122:tert-Butylhydroperoxide; C003959:TEMPO