Antigenic activity of three chimeric synthetic peptides of the transmembrane (gp41) and the envelope (gp120) glycoproteins of HIV-1 virus.
The antigenicity of three chimeric synthetic peptides (Qm, Qm-16, and Qm-17) incorporating an immunodominant epitope of the gp41 transmembrane protein (587-617) and the different epitopes of the gp120 envelope protein (495-516), (301-335), (502-516) of human immunodeficiency virus (HIV-1), separated by two glycine residues, was evaluated by UltramicroEnzyme-linked immunosorbent assay (UMELISA) by using panels of anti-HIV-1 positive sera (n = 47). The specificity was evaluated with samples from healthy blood donors (n = 20) and anti-HIV-2 positive samples (n = 10). The results indicate that the chimeric peptide, Qm, was the most reactive one because it detected antibodies to virus efficiently. This may be related to peptide adsorption onto the solid surface, the C-terminal region of HIV-1 gp120 (495-516) combined with gp41 (587-617) in the chimera, and the epitope accessibility to the antibodies. This study showed the usefulness of the chimeric peptides as antigen to detect antibodies to HIV-1 virus.
Marin
Milenen Hernández
MH
Department of Peptide Synthesis, Immunoassay Center, Havana, Cuba. iqpeptidos@cie.sld.cu
Peña
Lilliam Pozo
LP
Tanty
Chryslaine Rodríguez
CR
Higginson Clarke
David
D
Arenas
Martha Amat
MA
Noguerol
Kenia Ruenes
KR
León
Carlos Silva
CS
D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000276:immunology; D015658:HIV Infections / Q000097:blood*; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000276:immunology
D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D010455:Peptides