Antigenic mixture of synthetic peptides for the immunodiagnosis of HTLV I/II infection.
Monomeric and chimeric synthetic peptides were used as coating antigens in four different mixtures in a solid phase immunoassay to select an optimal combination for the detection of antibodies to human T-cell lymphotropic virus (HTLV) in serum samples. The peptides, P-13 (gp21 I), Q5 (gp21 II)-GG-(gp46 II), and Q (gp46 I)-GG-(p19 I), represent immunodominant sequences from transmembrane protein (gp21), envelope protein (gp46), and core protein (p19) of HTLV I/II viruses; they were the most antigenic and specific peptides in previous studies. The sequences of the chimeric peptides were separated by two glycine residues. An indirect UltramicroEnzyme-linked immunosorbent assay (UMELISA) was used to evaluate the antigenicity of these peptide mixtures by using samples from anti-HTLV I/II PRP205(M), (n = 20), HTLV I-infected individuals from Cuba (n = 7), and HTLV I-positive sera from Colombia and Chile (n = 9). The specificity was evaluated with healthy blood donor sera (n = 300), anti-HIV 1-positive samples (n = 10), and other seropositive samples to different infectious agents. The highest sensitivity and specificity was obtained with mixture 1, which could be very useful in the immunodiagnostic of HTLV infection.
Department of Retroviruses, National Center for Scientific Research, Havana, Cuba. iqvih@cie.sld.cu
Yadaris Márquez
YM
Bocalandro
Milenen Hernández
MH
Marin
René Antonio Vázquez
RA
Vallejo
Lilliam Pozo
LP
Peña
Carlos Silva
CS
León
Grisell Ramos
GR
Martínez
Chryslaine Rodríguez
CR
Tanty
David Higginson
DH
Clarke
Senia Bermúdez
SB
Velázquez
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015481:HTLV-I Antibodies / Q000097:blood / Q000276:immunology*; D015486:HTLV-I Antigens / Q000737:chemistry / Q000276:immunology*; D015490:HTLV-I Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D015482:HTLV-II Antibodies / Q000097:blood / Q000276:immunology*; D015487:HTLV-II Antigens / Q000737:chemistry / Q000276:immunology*; D015491:HTLV-II Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D012680:Sensitivity and Specificity
D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; D015482:HTLV-II Antibodies; D015487:HTLV-II Antigens; D010455:Peptides