Control of 4E-BP1 expression in mouse brown adipose tissue by the beta3-adrenoceptor.
Knockout of the translation inhibitor 4E-BP1 induces an overexpression of uncoupling protein-1 (UCP1) [Nature Medicine 7 (2001) 1128]. A possible inverse control of UCP1 and 4E-BP1 expressions in mouse brown adipose tissue was investigated. Cold-exposure, which increases the expression of UCP1, decreased that of 4E-BP1 mRNA in wild type but not in beta1/beta2/beta3-adrenoceptor knockout mice. Administration of the beta3-adrenoceptor agonist CL 316246 decreased 4E-BP1 mRNA by 75% and protein by 41% after 6 and 48 h, respectively. Our data are the first report of a regulation by the beta3-adrenoceptor of 4E-BP1 expression. They support a role of the latter in adaptive thermogenesis.
Lehr
Lorenz
L
Department of Cell Physiology and Metabolism, Centre Médical Universitaire, 1, rue Michel-Servet, 1211 Geneva 4, Switzerland. lehr6@etu.unige.ch
Kuehne
Françoise
F
Arboit
Patrizia
P
Giacobino
Jean-Paul
JP
Poulin
Francis
F
Muzzin
Patrick
P
Jimenez
Maria
M
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D003080:Cold Temperature; D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000187:drug effects; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D000318:Adrenergic beta-Agonists; C104151:EIF4EBP2 protein, human; D039642:Eukaryotic Initiation Factors; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3