[Effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats].
OBJECTIVE
To study the effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats.


METHODS
Different doses of permethrin and deltamethrin in corn oil were administered to rats by gavage for ten days, then PLC-Fluorescence detection was used to analyzed the contents of dopamine (DA), DOPAC and HVA in the Substantial Nigra and Striatum. The activities of TH were also detected by HPLC-Fluorescence detection. TH mRNA or TH protein levels were measured by RT-PCR and immunohistochemistry method.


RESULTS
Contents of DA in Striatum in 1/10LD50 DM group have a significant change, the contents of DOPAC and the turnover rate of DA all increased (P < 0.05), but DA, DOPAC and (DOPAC + HVA)/DA did not have any significant change in Substantial Nigra. The activities and mRNA level of TH had obvious decrease in two DM groups, the TH protein levels also significantly decreased in 1/10LD50 DM group.


CONCLUSIONS
Deltamethrin could decrease the activities, mRNA and protein levels in nigraostriatum of rats.
Tongji Medical College of Huazhong Science and Technology University, Wuhan 430030, China.
Gongping
G
Liu
Nian
N
Shi
Ronghong
R
Ma
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D006719:Homovanillic Acid / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D009570:Nitriles / Q000494:pharmacology*; D026023:Permethrin / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012333:RNA, Messenger; D015102:3,4-Dihydroxyphenylacetic Acid; C017180:decamethrin; D026023:Permethrin; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D006719:Homovanillic Acid