Detection of CMV infected cells by flow cytometry--evaluation of MAbs CCH2 and AAC10 directed against early and late CMV antigens.
In this work evaluation of usefulness of monoclonal antibodies (MAbs) CCH2 and AAC10 directed against early--pUL44(DB52) and late--ppUL83(pp65) CMV antigens, utilized in Department of Virology, NIH for routine diagnosis of CMV infection by shell vial and pp65 antigenemia assay, for determination of CMV antigens by flow cytometry in human leucocytes, isolated, infected and cultivated in vitro was presented.
Department of Virology, National Institute of Hygiene, 00-791 Warsaw, Chocimska 24, Poland.
Joanna
J
Siennicka
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000821:virology; D010750:Phosphoproteins / Q000032:analysis*; D014763:Viral Matrix Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa