[Effects of 1alpha,25-dihydroxyvitamin D(3) and UVB on cell proliferation and melanin synthesis of cultured human melanocyte].
OBJECTIVE
To study the effects of 1alpha,25-dihydroxyvitamin D(3) and UVB on cell proliferation and melanin synthesis of normal human melanocytes.


METHODS
Melanocytes of foreskins of healthy men were cultured and treated with various concentration of 1alpha,25-dihydroxyvitamin D(3) or UVB (55 mJ/cm(2)),or both. Cell proliferation was measured with MTT assay .The synthesis of melanin was determined by chromatography.


RESULTS
1alpha,25-dihydroxyvitamin D(3) and UVB could promote the proliferation of cultured melanocyte,and 1alpha,25-dihydroxyvitamin D(3) could promote the melanin synthesis. The significant concentration of 1alpha,25-dihydroxyvitamin D(3) ranging from 10(-7) to 10(-10) mol/L.


CONCLUSIONS
1alpha,25-dihydroxyvitamin D(3) and UVB irradiation could promote the proliferation of melanocyte,which indicates that they might be effective in the treatment of vitiligo.
Department of Dermatology, Peking University People's Hospital, Beijing 100044, China. xuqianxi@sohu.com
Qian-xi
QX
Xu
Juan
J
Du
Pei-ying
PY
He
Jian-zhong
JZ
Zhang
Tie-jun
TJ
Zhu
D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
D008543:Melanins; C118756:dihydroxy-vitamin D3; D014807:Vitamin D