[Identification of the triploid hybrid chromosomes of Elymus canadensis L. x Hordeum brivisubulatum Link. by genomic in situ hybridization].
AThe intergeneric F1 hybrid between Elymus canadensis L. and Hordeum brivisubulatum Link. is a triploid (2n = 3x = 21 ), in which 7 chromosomes,that is equal to the base number of parent's chromosomes, were lost. In order to indentify the chromosome constitution of the triploid F1 hybrid of E. canadensis L. x H. brivisubulatum Link., the genomic in situ hybridization of F1 root tip cell chromosome DNA that H. brivisubulatum H1 H1 H2H2 genome DNA labeled with Biotin-16-dUTP was conducted using E. canadensis SSH(c)H(c) genome as blocking DNA. The result showed that the chromosomes of triploid F1 hybrid consisted of 7 chromosomes from E. canadensis S genome and 14 chromosomes from H. brivisubulatum H1 H2 genome while the 7 chromosomes of Hc genome of E. canadensis were lost.
Agronomy College of Inner Mongolia Agricultural University, Hohhot 010019, China. yz_yz5810@sina.com
Zhuo
Z
Yu
Jin-Feng
JF
Yun
You-Zhi
YZ
Ma
Zhi-Yong
ZY
Xin
D002678:Chimera; D032461:Chromosomes, Plant; D031749:Elymus / Q000235:genetics*; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D011123:Polyploidy