Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform.
We implemented 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled asparagine-linked glycan (N-glycan) profiling on a microfluidic electrophoresis platform. Using 11.5 cm effective length etched channels and 4% linear polyacrylamide as the separation matrix, the major N-glycans in human serum were profiled in 12 min with a resolution comparable to what is achieved for these analytes on gel-based DNA sequencers. This demonstration suggests a practical clinical application for high-speed compact analyzers which might be uniquely based on microfluidic devices.
Department of Molecular Biomedical Research, Ghent University and VIB, Ghent, Belgium.
Nico
N
Callewaert
Roland
R
Contreras
Luba
L
Mitnik-Gankin
Loucinda
L
Carey
Paul
P
Matsudaira
Daniel
D
Ehrlich
D019075:Electrophoresis, Capillary / Q000379:methods*; D044085:Microfluidics; D011134:Polysaccharides / Q000097:blood*; D011721:Pyrenes / Q000737:chemistry
C103430:8-aminopyrene-1,3,6-trisulfonic acid; D011134:Polysaccharides; D011721:Pyrenes