Protective effects of the Chinese prescription Hachimi-jio-gan against diabetic oxidative stress.
We used rats with streptozotocin (STZ)-induced diabetes to investigate the effects of Hachimi-jiogan on diabetic oxidative stress. Oral administration of Hachimi-jio-gan, at a dose of 50, 100 or 200 mg kg(-1) (body weight) daily, for 10 days to rats with STZ-induced diabetes resulted in significant dose-dependent decreases in serum levels of glucose and glycosylated protein, implying that Hachimi-jio-gan improves the abnormal glucose metabolism that leads to oxidative stress. Hachimi-jio-gan also showed a tendency to reduce the urine volume and significantly reduced the elevated urinary protein level. Moreover, rats with STZ-induced diabetes had high serum levels of superoxide and nitrite/nitrate. However, the administration of Hachimi-jio-gan dose-dependently reduced the overproduction of radicals associated with diabetes, suggesting the role of Hachimijio-gan as a radical scavenger that could protect against diabetic oxidative stress. Furthermore, thiobarbituric acid-reactive substance levels in serum and hepatic and renal mitochondria were dose-dependently lower in the Hachimi-jio-gan-treated groups than in the control diabetic group, which implies that Hachimi-jio-gan would alleviate the oxidative stress associated with diabetes through the inhibition of lipid peroxidation. These results indicate that Hachimi-jio-gan is a potential therapeutic agent that will reduce the damage caused by oxidative stress involved in diabetes.
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Hyun Young
HY
Kim
Takako
T
Yokozawa
Eun Ju
EJ
Cho
Noriko
N
Yamabe
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D006023:Glycoproteins / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013481:Superoxides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects; D000093422:Glycated Serum Proteins
D001786:Blood Glucose; D001798:Blood Proteins; D004365:Drugs, Chinese Herbal; D006023:Glycoproteins; D009566:Nitrates; D009573:Nitrites; C054591:hachimijiogan; D013481:Superoxides; D013311:Streptozocin; D003404:Creatinine; D000093422:Glycated Serum Proteins