Effects of flanking genes on the phenotypes of mice deficient in basigin/CD147.
The induction of null mutations by means of homologous recombination is a powerful technique for clarifying the biological activities of target genes. However, the problems of the genetic background and flanking genes should be borne in mind. Here we employed a breeding strategy to compare three lines of mice deficient in the basigin (Bsg)/CD147 gene. The first line was F2 from F1 hybrid offspring of the 129/SV chimera and C57BL/6J. The second one was from a C57BL/6J congenic line. Both lines showed high embryonic lethality, sterility, and blindness. The third one was 'reverse F2' from 'reverse F1' hybrid offspring of the C57BL/6J congenic line and 129/SV. Surprisingly, this line showed a normal birth rate, while sterility and blindness persisted. Our results clearly separate the effects of the induced null mutation from those of flanking genes and the genetic background, and provide a useful means of investigating the biological functions of Bsg.
Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Sen
S
Chen
Kenji
K
Kadomatsu
Mineo
M
Kondo
Yoshiro
Y
Toyama
Kiyotaka
K
Toshimori
Shinji
S
Ueno
Yozo
Y
Miyake
Takashi
T
Muramatsu
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D051926:Basigin; D001723:Birth Rate; D001766:Blindness / Q000235:genetics; D001947:Breeding; D002678:Chimera; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000473:pathology / Q000502:physiology; D005260:Female; D018390:Gene Targeting; D007246:Infertility / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D010375:Pedigree; D010641:Phenotype; D011995:Recombination, Genetic; D012160:Retina / Q000166:cytology / Q000473:pathology / Q000503:physiopathology; D013090:Spermatocytes / Q000166:cytology / Q000473:pathology; D015996:Survival Rate
D015703:Antigens, CD; C497887:Bsg protein, mouse; D051926:Basigin