High-level expression of the neutralizing epitope of porcine epidemic diarrhea virus by a tobacco mosaic virus-based vector.
Porcine epidemic diarrhea virus (PEDV) causes acute enteritis in pigs of all ages and is often fatal for neonates. A tobacco mosaic virus (TMV)-based vector was utilized for the expression of a core neutralizing epitope of PEDV (COE) for the development of a plant-based vaccine. In this study, the coding sequence of a COE gene was optimized based on the modification of codon usage in tobacco plant genes and the removal of mRNA-destabilizing sequences. The native and synthetic COE genes were cloned into TMV-based vectors and expressed in tobacco plants. The recombinant COE protein constituted up to 5.0% of the total soluble protein in the leaves of tobacco plants infected with the TMV-based vector containing synthetic COE gene, which was approximately 30-fold higher than that in tobacco plants infected with TMV-based vector containing a native COE gene. Therefore, this result indicates that the plant viral expression system with a synthetic gene optimized for plant expression is suitable to produce a large amount of antigen for the development of plant-based vaccine rapidly.
Institute of Basic Science, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Tae-Jin
TJ
Kang
Kui-Hyeon
KH
Kang
Jin-Ah
JA
Kim
Tae-Ho
TH
Kwon
Yong-Suk
YS
Jang
Moon-Sik
MS
Yang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine / Q000821:virology; D014026:Nicotiana / Q000235:genetics; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D000939:Epitopes; D011994:Recombinant Proteins; D014764:Viral Proteins