HPLC determination of four active saponins from Panax notoginseng in rat serum and its application to pharmacokinetic studies.
Four main active saponins (ginsenosides Rg1, Rb1, Rd and notoginsenoside R1) in Panax notoginseng in rat serum after oral and intravenous administration of total saponins of P. notoginseng (PNS) to rats were determined using a simple and sensitive high-performance chromatographic method. The serum samples were pretreated with solid-phase extraction before analysis. The calibration curves for the four saponins were linear in the given concentration ranges. The intra-day and inter-day assay coefficients in serum were less than 10.0% and the recoveries of the method were higher than 80.0% in the high, middle and low concentrations. This method was applied to study the pharmacokinetics following oral and intravenous administration of PNS.
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, People's Republic of China.
Lie
L
Li
Yuxin
Y
Sheng
Jinlan
J
Zhang
Chuanshe
C
Wang
Dean
D
Guo
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D005894:Panax / Q000737:chemistry*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity
D036145:Ginsenosides; D012503:Saponins; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1; C049863:ginsenoside Rd; C072936:notoginsenoside R1