[Therapeutic effect of 125IUdR on the glioma cell line C6 in vitro and in vivo].
OBJECTIVE
To investigate the therapeutic effect of 125IUdR on gliomas.


METHODS
By means of growth-curve, clonogenic formation experiment and MTT assay, the inhibitive effect of 125IUdR on the proliferation of C6 cells was studied in vitro. Wistar rats with intracerebral C6 gliomas were used to verify the therapeutic efficacy of 125IUdR in vivo.


RESULTS
C6 monolayer cells were efficiently inhibited by 125IUdR in a time-and-dose-dependent manner. In the MTT assay, after treament with 150 kBq/ml 125IUdR for 5 days, the inhibition rate reached 93.06%. In murine transplantable tumor, 125IUdR had significant therapeutic effect on rats bearing solid tumor glioma C6. After treatment with 125IUdR for 5 days, the tumor weight of experiment group was lower than that of blank group and control group (P<0.01). The median survival of animals treated with 125IUdR (27 days) was markedly longer than that of control group (9 days) (P<0.01). Na 125I and 127IUdR showed little inhibitive effect on the proliferation of C6 cells in vitro and in vivo.


CONCLUSIONS
125IUdR can markedly inhibit the growth of Glioma cell line C6. 125IUdR has potential for the treatment of malignant brain tumor.
Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu 610041, China.
Bo
B
Wu
Chao
C
You
Xiao-dong
XD
Liu
Bo-wen
BW
Cai
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D005910:Glioma / Q000473:pathology / Q000532:radiotherapy*; D007065:Idoxuridine / Q000627:therapeutic use*; D007457:Iodine Radioisotopes / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D007065:Idoxuridine