Thyroid transcription factor-1 immunocytochemical staining of pleural fluid cytocentrifuge preparations for detection of small cell lung carcinoma.
OBJECTIVE
To assess whether thyroid transcription factor-1 (TTF-1) immunostaining of cytocentrifuge slides previously stained by the Papanicolaou technique is feasible and useful in diagnosing small cell lung carcinoma (SCLC) in pleural fluid.


METHODS
Eleven Papanicoalou-stained cytocentrifuge preparations of pleural fluid from 11 patients with SCLC were reviewed. The initial cytologic diagnoses were 5 positive, 1 suspicious and 5 negativefor SCLC. After transferring the cytologic material to a positively charged slide, immunostaining was performed using mouse monoclonal antibody against TTF-1.


RESULTS
The neoplastic cellularity varied among the single suspicious and 5 positive cases. The case with a suspicious diagnosis had scant tumor cells. All 6 cases showed nuclear stainingfor TTF-1 in most of the tumor cells. The other 5 cases with a negative cytologic diagnosis were negativefor TTF-1.


CONCLUSIONS
The results of this study indicate that immunostaining for TTF-1 can be successfully applied to previously Pap-stained cytocentrifuge preparations to assist in the diagnosis of SCLC in pleural fluid.
Liu
Jing
J
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston Medical School, Houston, Texas 77030, USA. jing.liu.1@uth.tmc.edu
Farhood
Anwar
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000473:pathology*; D002498:Centrifugation / Q000295:instrumentation / Q000379:methods; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D016066:Pleural Effusion, Malignant / Q000378:metabolism / Q000473:pathology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012189:Retrospective Studies; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D004396:Coloring Agents; C000614412:NKX2-1 protein, human; C000614414:Nkx2-1 protein, mouse; D009687:Nuclear Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors