Evidence for polyadenylated mRNA in Pseudomonas aeruginosa.
In this paper, we report the synthesis of Pseudomonas aeruginosa cDNA in the presence of oligo(dT) primers. Hybridization of oligonucleotide DNA microarrays indicates that under the experimental conditions used, at least 43.7% of the expressed genes from P. aeruginosa PAO1, representing many different functional classes, can be detected by using oligo(dT)-primed cDNAs.
Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany.
Senthil Selvan
SS
Saravanamuthu
Franz
F
von Götz
Prabhakar
P
Salunkhe
Rathinam
R
Chozhavendan
Robert
R
Geffers
Jan
J
Buer
Burkhard
B
Tümmler
Ivo
I
Steinmetz
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011061:Poly A / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; C027903:oligo (dT); D011061:Poly A