Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB.
Clinical and environmental strains of Burkholderia cenocepacia belonging to the recA lineages IIIA and IIIB were examined for exopolysaccharide (EPS) production. The exopolysaccharides structure was determined using mainly gas chromatography coupled to mass spectrometry and NMR spectroscopy. All the strains produced Cepacian, a highly branched polysaccharide constituted of a heptasaccharide repeating unit, composed of one rhamnose, one glucose, one glucuronic acid, one mannose and three galactose residues. This polymer is the most common exopolysaccharide produced by strains of the Burkholderia cepacia (Bcc) complex. One clinical strain produced also another polysaccharide constituted of three galactose units and one 3-deoxy-D-manno-2-octulosonic acid residues, a polymer that was previously isolated from two strains of B. cepacia genomovar I and B. cenocepacia IIIA.
Unità Biotecnologie, C.R. Casaccia, ENEA, 00060 Rome, Italy.
Luigi
L
Chiarini
Paola
P
Cescutti
Laura
L
Drigo
Giuseppe
G
Impallomeni
Yury
Y
Herasimenka
Annamaria
A
Bevivino
Claudia
C
Dalmastri
Silvia
S
Tabacchioni
Graziana
G
Manno
Flavio
F
Zanetti
Roberto
R
Rizzo
D016956:Burkholderia cepacia / Q000145:classification / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D011135:Polysaccharides, Bacterial