Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations.
Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.
Institut für Hygiene, Universitätsklinikum Münster, Münster, Germany.
Alexander
A
Mellmann
Jan
J
Mosters
Edda
E
Bartelt
Peter
P
Roggentin
Andrea
A
Ammon
Alexander W
AW
Friedrich
Helge
H
Karch
Dag
D
Harmsen
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D002417:Cattle; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005408:Flagellin / Q000235:genetics*; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA
D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin