Cell cycle regulation of the murine 8-oxoguanine DNA glycosylase (mOGG1): mOGG1 associates with microtubules during interphase and mitosis.
8-Oxoguanine DNA glycosylase (OGG1) is a major DNA repair enzyme in mammalian cells. OGG1 participates in the repair of 8-oxoG, the most abundant known DNA lesion induced by endogenous reactive oxygen species in aerobic organisms. In this study, antibodies directed against purified recombinant human OGG1 (hOGG1) or murine (mOGG1) protein were chemically conjugated to either the photosensitizer Rose Bengal or the fluorescent dye Texas red. These dye-protein conjugates, in combination with binding assays, were used to identify associations between mOGG1 and the cytoskeleton of NIH3T3 fibroblasts. Results from these binding studies showed that mOGG1 associates with the cytoskeleton by specifically binding to the centriole and microtubules radiating from the centrosome at interphase and the spindle assembly at mitosis. Similar results were obtained with hOGG1. Together results reported in this study suggest that OGG1 is a microtubule-associated protein itself or that OGG1 utilizes yet to be identified motor proteins to ride on microtubules as tracks facilitating the movement and redistribution of cytoplasmic OGG1 pools during interphase and mitosis and in response to oxidative DNA damage.
Department of Pharmacological Sciences, School of Medicine, University Hospital and Medical Center, State University of New York, Stony Brook, New York 11794-8651, USA.
Kimberly A
KA
Conlon
Dmitry O
DO
Zharkov
Miguel
M
Berrios
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D001667:Binding, Competitive; D002453:Cell Cycle / Q000502:physiology*; D002502:Centrioles / Q000276:immunology / Q000378:metabolism; D045647:DNA Glycosylases / Q000032:analysis / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006801:Humans; D007399:Interphase / Q000502:physiology; D051379:Mice; D008870:Microtubules / Q000276:immunology / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology; D041681:NIH 3T3 Cells; D010782:Photolysis; D012395:Rose Bengal / Q000032:analysis / Q000737:chemistry; D008941:Spindle Apparatus / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism; D014966:Xanthenes / Q000032:analysis / Q000737:chemistry
D000906:Antibodies; D005456:Fluorescent Dyes; D014404:Tubulin; D014966:Xanthenes; D012395:Rose Bengal; C034657:Texas red; D045647:DNA Glycosylases; C485990:Ogg1 protein, mouse; C479058:oxoguanine glycosylase 1, human