Gene frequency distribution of the BoLA-DRB3 locus in Saavedreño Creole dairy cattle.
The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he = 0.919). The FIS index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedreño Creole dairy cattle, with significant variation in allele frequency among cattle breeds.
Centro de Investigaciones en Genética Básica y Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, CC 296, B1900AVW La Plata, Argentina.
M V
MV
Ripoli
J P
JP
Lirón
J C
JC
De Luca
F
F
Rojas
F N
FN
Dulout
G
G
Giovambattista
D000483:Alleles; D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D005787:Gene Frequency; D016678:Genome; D005838:Genotype; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
C423282:BoLA-DRB3 antigen; D000949:Histocompatibility Antigens Class II; D004247:DNA