New technologies for the human cytome project.
Cytomes or cell systems are composed of various kinds of single-cells and constitute the elementary building units of organs and organisms. Their individualised (cytomic) analysis overcomes the problem of averaged results from cell and tissue homogenates where molecular changes in low frequency cell populations may be hidden and wrongly interpreted. Analysis of the cytome is of pivotal importance in basic research for the understanding of cells and their interrelations in complex environments like tissues and in predictive medicine where it is a prerequisite for individualised preventive therapy. Analysis of molecular phenotypes requires instrumentation that on the one hand provides high-throughput measurement of individual cells and is on the other hand highly multiplexed, enabling the simultaneous acquisition of many parameters on the single cell level. Upcoming technology suitable to this task, such as slide based cytometry is available or under development. The realisation of cytomic technology is important for the realisation of the human cytome project.
Pediatric Cardiology, Heart Center Leipzig GmbH, University Hospital Leipzig, Leipzig, Germany. tarnok@medizin.uni-leipzig.de
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D003585:Cell Biology / Q000639:trends*; D019295:Computational Biology; D003584:Cytological Techniques; D003592:Cytophotometry; D004586:Electrophoresis; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics; D013194:Staining and Labeling