Extracellular matrix-like surfactant polymers containing arginine-glycine-aspartic acid (RGD) peptides.
We report on a novel series of biomimetic polymers exhibiting interfacial properties similar to the extracellular matrix. A series of well-defined surfactant polymers were synthesized by simultaneously incorporating arginine-glycine-aspartic acid (RGD) peptide, dextran oligosaccharide, and hexyl ligands with controlled feed ratios onto a poly(vinyl amine) (PVAm) backbone. The peptide sequence was H-GSSSGRGDSPA-NH(2) (Pep) having a hydrophilic extender at the amino terminus and capped carboxy terminus. The peptide-to-dextran (Pep:Dex) ratios were varied to create surfactants having 0, 25, 50, 75, and 100 mol-% peptide relative to dextran. The surfactants were characterized by IR, NMR and atomic force microscopy (AFM) for composition and surface active properties. AFM confirmed full surface coverage of PVAm(Pep)(100%) on graphite, and supported the mechanism of interdigitation of hexyl ligands between surfactant molecules within a specified range of hexyl chain densities. the attachment and growth of human pulmonary artery endothelial cells on the PVAm(Pep)(100%) surface was identical to the fibronectin positive control. Cell adhesion decreased dramatically with decreasing peptide density on the surfactant polymers. Molecular model of a peptide surfactant polymer, consisting of poly(vinyl amine) backbone with peptide, dextran oligosaccharide and hexyl branches coupled to the polymer chain.
Department of Macromolecular Science, Wickenden 201, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-7207, USA.
Eric H
EH
Anderson
Mark A
MA
Ruegsegger
Gurunathan
G
Murugesan
Kandice
K
Kottke-Marchant
Roger E
RE
Marchant
D000042:Absorption; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002448:Cell Adhesion; D002455:Cell Division; D042783:Endothelial Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D011651:Pulmonary Artery / Q000166:cytology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
D009842:Oligopeptides; D011108:Polymers; D013501:Surface-Active Agents; C047981:arginyl-glycyl-aspartic acid