Hypertension attenuates cell-to-cell communication in hamster retractor muscle feed arteries.
This study examined whether hypertension attenuated cell-to-cell communication in skeletal muscle resistance arteries. Briefly, arteries feeding the retractor muscle of normotensive and hypertensive hamsters were cannulated, pressurized, and superfused with a physiological saline solution. Cell-to-cell communication was functionally assessed by application of vasoactive stimuli (via micropipette) to a small portion of a feed artery while diameter at sites distal to the point of agent application was monitored. In keeping with past observations, discrete application of a smooth muscle depolarizing agent (phenylephrine or KCl) elicited a localized vasoconstriction that conducted poorly along feed arteries from normotensive hamsters. In contrast, acetylcholine, an agent known to hyperpolarize endothelial cells, elicited a vasodilation in normotensive feed arteries that conducted with little decay. Whereas smooth muscle depolarizing agents continued to elicit a localized response, conduction of endothelium-dependent vasodilation was attenuated in hypertensive hamsters. This decrease occurred in the absence of changes in vessel reactivity to intravascular pressure or to global application of phenylephrine, U-46619, or acetylcholine. We propose, on the basis of these physiological observations, quantitative mRNA measurements of connexins 37, 40, 43, and 45, and analysis of the literature, that an increase in endothelial-to-endothelial or smooth muscle-to-endothelial coupling resistance is likely responsible for hypertension-induced impairment in vascular communication. We hypothesize that this attenuation could contribute to the rise in total peripheral resistance characteristically observed in hypertension.
Department of Biological Sciences, Ohio University, Athens, Ohio, USA.
David T
DT
Kurjiaka
Shawn B
SB
Bender
Darin D
DD
Nye
William B
WB
Wiehler
Donald G
DG
Welsh
D000818:Animals; D001158:Arteries / Q000166:cytology* / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D017630:Connexins / Q000235:genetics; D006224:Cricetinae; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D017629:Gap Junctions / Q000502:physiology; D006973:Hypertension / Q000378:metabolism / Q000503:physiopathology*; D018482:Muscle, Skeletal / Q000098:blood supply*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014664:Vasodilation / Q000502:physiology
D017630:Connexins; D012333:RNA, Messenger