Larval melanocyte regeneration following laser ablation in zebrafish.
A method to specifically ablate melanocytes in a genetically tractable organism would facilitate the analysis of melanocyte regeneration and regulation. We have demonstrated that a Q-switched neodymium:yttrium-aluminum-garnet dermatology laser kills larval melanocytes in zebrafish. Following melanocyte ablation, new melanocytes regenerate from unpigmented precursors. We show that melanocyte regeneration following laser ablation requires kit receptor tyrosine kinase.
Department of Genetics, Division of Dermatology, Washington University School of Medicine, St Louis, Missouri, USA.
Chao-Tsung
CT
Yang
Roberta D
RD
Sengelmann
Stephen L
SL
Johnson
D000818:Animals; D002455:Cell Division / Q000502:physiology; D007834:Lasers; D008544:Melanocytes / Q000166:cytology* / Q000502:physiology*; D019009:Proto-Oncogene Proteins c-kit / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000254:growth & development; D012879:Skin Physiological Phenomena; D012880:Skin Pigmentation / Q000502:physiology; D015027:Zebrafish
D019009:Proto-Oncogene Proteins c-kit