A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.
Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Eliana B
EB
Gómez
Vanessa T
VT
Angeles
Susan L
SL
Forsburg
D000483:Alleles; D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; D002453:Cell Cycle; D019926:Cyclin B / Q000172:deficiency; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005816:Genetic Complementation Test; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D010957:Plasmids; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D011503:Transglutaminases / Q000737:chemistry / Q000235:genetics
D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; C577398:CUT9 protein, S pombe; D019926:Cyclin B; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C082463:RAD4 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D011503:Transglutaminases