graal: a Drosophila gene coding for several mosaic serine proteases.
Serine proteases play vital roles in several biological processes such as development and immunity. We have characterized Graal, a large multi-domain serine protease from Drosophila. Graal is spliced in at least three transcripts that are present throughout development. The domains found in Graal proteins are: chitin-binding domains (CBD), scavenger receptor cysteine-rich (SRCR) domains, low density lipoprotein receptor cysteine-rich (LDLR-CR) domains, histidine and proline-rich domains, a NGGYQPP-repeat domain and a serine protease domain. The last 2370 nucleotides of these RNAs are identical and encode a His-rich domain, two SRCR domains, two LDLR-CR domains and a protease domain. The transcription of graal is upregulated after fungal or bacterial infection. Analysis of the Iso1 (y;cn,sp,bw) strain shows that graal transcription is impaired in this fly line due to the insertion of a retrotransposon in the sixth exon. However, no phenotype could be observed consecutive to the absence of graal full length transcripts, particularly in the context of an immune challenge.
Munier
Anne Isabelle
AI
Institut de Biologie Moléculaire et Cellulaire, CNRS UPR 9022, 15 rue Descartes, 67084 Strasbourg, France.
Medzhitov
Ruslan
R
Janeway
Charles A
CA
Doucet
Daniel
D
Capovilla
Maria
M
Lagueux
Marie
M
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D017344:Genes, Insect; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D012697:Serine Endopeptidases