HPLC determination and pharmacokinetic studies of salvianolic acid B in rat plasma after oral administration of Radix Salviae Miltiorrhizae extract.
A precise and reproducible HPLC method has been established and validated for determination of salvianolic acid B (SalB) in rat plasma after oral administration of Radix Salviae Miltiorrhizae extract. Liquid-liquid extraction was adopted for the sample preparation. Separation was accomplished on a C(18) column with a linear gradient elution consisting of acetonitrile and aqueous phosphoric acid. Ultraviolet detection was at 280 nm. The method was validated over the concentration range 10.8-259.4 microg/mL using 1 mL of plasma. The assay was linear over this concentration range with a coefficient of variation less than 7%. The extraction recovery of SalB was within the range 71-83% with RSD 11%. The mean recovery of the internal standard was 84% (n = 6) with RSD of 5.6%. This method is suitable to determine SalB in plasma and to investigate the pharmacokinetics of SalB.
Zhang
Jinlan
J
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, People's Republic of China.
Yu
Hailan
H
Sheng
Yuxin
Y
Li
Lie
L
Ye
Min
M
Guo
Dean
D
D000097:Acetonitriles; D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000097:blood / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D008297:Male; D010756:Phosphoric Acids; D010936:Plant Extracts; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza; D012680:Sensitivity and Specificity; D053630:Depsides
D000097:Acetonitriles; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D010756:Phosphoric Acids; D010936:Plant Extracts; C076944:salvianolic acid B; D053630:Depsides; C030242:phosphoric acid; C032159:acetonitrile