The generation of cloned Drosophila melanogaster.
We report here the first successful use of embryonic nuclear transfer to create viable adult Drosophila melanogaster clones. Given the generation time, cost effectiveness, and relative ease of embryonic nuclear transplant in Drosophila, this method can provide an opportunity to further study the constraints on development imposed by transplanting determined or differentiated nuclei.
Department of Biology, Dalhousie University, Halifax, Nova Scotia B4H 4J1, Canada.
Andrew J
AJ
Haigh
William A
WA
MacDonald
Vett K
VK
Lloyd
D000818:Animals; D018929:Cell Culture Techniques; D002999:Clone Cells; D019976:Cloning, Organism / Q000191:economics / Q000379:methods*; D004247:DNA / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology; D005819:Genetic Markers; D053652:Nuclear Transfer Techniques; D019076:Transgenes
D004272:DNA, Mitochondrial; D005819:Genetic Markers; D004247:DNA