[Development of retrovirus-mediated insertional mutagenesis in zebrafish and its application in saturation mutagenesis and gene screening].
The strategies of large-scale mutagenesis and gene screening include chemical mutagenesis, insertional mutagenesis and gene trap. Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth. Gene trap elements are also used in the construction of retrovirus vectors. The whole system is likely to make zebrafish the first vertebrate to achieve saturation mutagenesis and gene screening.
College of Life Science, Peking University, Beijing 100871, China. qifei@pku.edu.cn
Fei
F
Qi
Shuo
S
Lin
Qi-Chang
QC
Fan
D000818:Animals; D002874:Chromosome Mapping; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional / Q000379:methods*; D012190:Retroviridae / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*