Cytoplasmic nonsense-mediated mRNA decay for a nonsense (W262X) transcript of the gene responsible for hereditary tyrosinemia, fumarylacetoacetate hydrolase.
Messenger RNAs containing premature stop codons are generally targeted for degradation through the nonsense-mediated mRNA decay (NMD) pathway. The subcellular localization of the NMD process in higher eukaryotes remains controversial. While many mRNAs are subjected to NMD prior to their release from the nucleus, a few display cytoplasmic NMD. To understand the possible impact of NMD on the pathogenesis of hereditary tyrosinemia type I, a severe metabolic disease caused by fumarylacetoacetate hydrolase (FAH) deficiency, we examined the metabolism of FAH mRNA harboring a nonsense mutation, W262X, in lymphoblastoid cell lines derived from patients and their parents. W262X-FAH transcripts show a approximately 20-fold reduction in abundance in mutant cells, which is translation-dependent. Cellular fractionation shows that this down-regulation of the W262X transcript occurs in the cytoplasm. Thus, the W262X FAH is another example of nonsense mRNAs subjected to the NMD pathway in the cytoplasm.
Dreumont
Natacha
N
Laboratory of Cellular and Developmental Genetics, CREFSIP, Department of Medicine, Université Laval, Que., Canada.
Maresca
Antonella
A
Khandjian
Edward W
EW
Baklouti
Faouzi
F
Tanguay
Robert M
RM
D002460:Cell Line; D018389:Codon, Nonsense; D005786:Gene Expression Regulation; D006801:Humans; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013347:Subcellular Fractions / Q000737:chemistry; D020176:Tyrosinemias / Q000235:genetics* / Q000378:metabolism
D018389:Codon, Nonsense; D012333:RNA, Messenger; D006867:Hydrolases; C020156:fumarylacetoacetase