Quinolinic acid induces NMDA receptor-mediated lipid peroxidation in rat brain microvessels.
Quinolinic acid increased the generation of lipid peroxidation products by isolated rat brain microvessels in vitro. The effect was inhibited both by a specific NMDA receptor antagonist D-2-amino-5-phosphonovaleric acid and by reduced glutathione (GSH). Furthermore, quinolinic acid displaced specific binding of [(3)H]-L-glutamate by cerebral microvessel membranes, particularly in the presence of NMDA receptor co-agonist (glycine) and modulator (spermidine). We conclude that quinolinic acid can cause potentially cytotoxic lipid peroxidation in brain microvessels via an NMDA receptor mediated mechanism.
St'astný
Frantisek
F
Prague Psychiatric Centre affiliated with Charles University 3rd Faculty of Medicine, Prague, Czech Republic.
Lisý
Václav
V
Mares
Vladislav
V
Lisá
Vera
V
Balcar
Vladimir J
VJ
Santamaría
Abel
A
D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D015763:2-Amino-5-phosphonovalerate; D005978:Glutathione