[Differential expression of motility-related protein-1/CD9 gene in gastric cancers and premalignant lesions].
OBJECTIVE
To clone and identify gastric cancer-related genes and explore the possible pathogenic mechanism of gastric cancer.


METHODS
The differentially expressed cDNA bands were isolated by fluorescent mRNA differential display in gastric cancer specimens, matched normal gastric mucosa and premalignant lesions. The motility-related protein-1 (MRP-1/CD9) gene was one of the down-regulated genes. MRP-1/CD9 gene expression in different kinds of gastric tissue was analyzed by Northern blot and reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
MRP-1/CD9 expression was down-regulated in all gastric cancer tissues. Northern blot analysis confirmed this differential expression. RT-PCR analysis showed that the MRP-1/CD9 gene expression was much lower in gastric cancers(0.31+/-0.18) than in the matched normal gastric tissue (0.49+/-0.24) and in the premalignant lesions (0.47+/-0.18) (P<0.05), and its expression in intestinal type gastric cancer (0.38+/-0.16) was higher than that in diffuse type gastric cancer (0.22+/-0.17) (P<0.05).


CONCLUSIONS
The MRP-1/CD9 gene expression was down-regulated in gastric cancer, its expression was probably related to the carcinogenesis and histology types of gastric cancer.
Zhang
Wei-ming
WM
Central Laboratory of Basic Medical SciencesTianjin Medical University,Tianjin, 300070 P.R.China. zj1978824@163.com
Zheng
Jie
J
Xu
Yao
Y
Liu
Wen-tian
WT
Li
Yan-yun
YY
Xing
Jun
J
Geng
Xin
X
D015703:Antigens, CD / Q000235:genetics*; D015152:Blotting, Northern; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D011230:Precancerous Conditions / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*; D060245:Tetraspanin 29
D015703:Antigens, CD; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29