Laccase from Melanocarpus albomyces binds effectively to cellulose.
A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
VTT Biotechnology, P.O. Box 1500, Fin-02044 VTT, Finland.
Laura-Leena
LL
Kiiskinen
Hetti
H
Palonen
Markus
M
Linder
Liisa
L
Viikari
Kristiina
K
Kruus
D000327:Adsorption; D001203:Ascomycota / Q000201:enzymology*; D002482:Cellulose / Q000378:metabolism*; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism*; D008956:Models, Chemical; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
D005656:Fungal Proteins; D011994:Recombinant Proteins; D002482:Cellulose; D042845:Laccase