Antioxidative responses of Calendula officinalis under salinity conditions.
To gain a better insight into long-term salt-induced oxidative stress, some physiological parameters in marigold (Calendula officinalis L.) under 0, 50 and 100 mM NaCl were investigated. Salinity affected most of the considered parameters. High salinity caused reduction in growth parameters, lipid peroxidation and hydrogen peroxide accumulation. Under high salinity stress, a decrease in total glutathione and an increase in total ascorbate (AsA + DHA), accompanied with enhanced glutathione reductase (GR, EC 1.6.4.2) and ascorbate peroxidase (APX, EC 1.11.1.11) activities, were observed in leaves. In addition, salinity induced a decrease in superoxide dismutase (SOD, EC 1.15.1.1) and peroxidase (POX, EC 1.11.1.7) activities. The decrease in dehydroascorbate reductase (DHAR, EC 1.8.5.1) and monodehydroascorbate reductase (MDHAR, EC 1.6.5.4) activities suggests that other mechanisms play a major role in the regeneration of reduced ascorbate. The changes in catalase (CAT, EC 1.11.1.6) activities, both in roots and in leaves, may be important in H2O2 homeostasis.
Chaparzadeh
Nader
N
Department of Biology, Teacher Training University, PO Box 15815-3587, Tehran, Iran.
D'Amico
Maria Lucia
ML
Khavari-Nejad
Ramazan-Ali
RA
Izzo
Riccardo
R
Navari-Izzo
Flavia
F
D000975:Antioxidants / Q000378:metabolism; D020838:Calendula / Q000187:drug effects / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D009994:Osmolar Concentration; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*
D000975:Antioxidants; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D005978:Glutathione; D019803:Glutathione Disulfide