Fluorescence in situ hybridization analysis of human oocytes: advantages of a double-labeling procedure.
OBJECTIVE
To improve the fluorescence in situ hybridization (FISH) chromosomal analysis of human oocytes and first polar bodies.


METHODS
In situ chromosomal identification on isolated cells, with combinations of centromeric (or locus-specific) probes and whole-chromosome painting probes for chromosomes 9, 13, 16, 18, 21, and X.


METHODS
Montpellier University Hospital.


METHODS
Women participating in an IVF program.


METHODS
Fifty-four in vitro unfertilized oocytes were fixed on slides, and simple or double FISH labeling procedures were performed on preparations.


METHODS
Simultaneous in situ visualization of specific domains and chromosome arms of each targeted chromosome.


RESULTS
Eight chromosomal abnormalities were identified, including two hyperhaploidies, three cases of extra single chromatid, and three cases of balanced separation of sister chromatids. Also, the double-labeling procedure allowed the avoidance of five interpretation errors, owing to additional artefactual signals.


CONCLUSIONS
By ensuring precise identification of both chromosomes and single chromatids, the FISH double-labeling procedure limits the risk of erroneous interpretation and allows a more accurate cytogenetic analysis of human oocytes.
Pellestor
Franck
F
CNRS-UPR 1142, Montpellier, France. Franck.Pellestor@igh.cnrs.fr
Anahory
Tal
T
Andréo
Brigitte
B
Régnier-Vigouroux
Gilles
G
Soulié
Jean Pierre
JP
Baudouin
Magalie
M
Demaille
Jacques
J
D002869:Chromosome Aberrations; D020223:Chromosome Painting / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics*; D005260:Female; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009865:Oocytes / Q000648:ultrastructure*
D005456:Fluorescent Dyes