[Research of the electrophysiological properties of acutely dissociated spiral ganglion neurons isolated from mice using patch clamp technique].
OBJECTIVE
To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice.


METHODS
Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique.


RESULTS
In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents. Ion channel activity had the character of voltage-dependence. Some cells, which did not exhibit sodium currents exhibited, delayed rectifier potassium currents.


CONCLUSIONS
The electrophysiological properties of acutely dissociated spiral ganglion neurons could be reference to the research of the mechanics of auditory propagation and the ion channel pharmacology.
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu 610041, China. cou68@163.tom.com
Jia-Hong
JH
Deng
Jian-Gang
JG
Fan
Chuan-Yu
CY
Liang
D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology; D005260:Female; D007473:Ion Channels / Q000502:physiology; D008297:Male; D051379:Mice; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D013136:Spiral Ganglion / Q000502:physiology*
D007473:Ion Channels