[The structure of a conserved region of porcine genome, represented in human genome by chromosome 17].
Radiation mapping of nine genes (H3F3B, HLR1, MYL4, STAT5B, THRA1, TOP2A, MCP1, NF1, and MPO) to porcine chromosome 12 was carried out. Also, subchromosomal location of the NF1 gene along with the two loci containing the DNA sequences homologous to the DNA of the two human BAC clones was determined. The NF1 position was ascertained via microdissection of chromosome 12 with subsequent PCR amplification of the gene fragment with specific primers. BAC clones were mapped using FISH. Comparative analysis of the gene order in porcine chromosome 12 and in the homologous human chromosome 17 was performed. It was demonstrated that the gene orders in these chromosomes differed relative to the position of the MPO gene.
Larkin
D M
DM
Astakhova
I M
IM
Kozhin
A Iu
AIu
Karamysheva
T V
TV
Rubtsov
N B
NB
Kuznetsov
S B
SB
Zhdanova
N S
NS
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D002886:Chromosomes, Human, Pair 17; D017931:DNA Primers; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016133:Polymerase Chain Reaction
D017931:DNA Primers