Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan.
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.
Department of Chemical Process and Environments, The University of Kitakyushu, 1-1 Hibikino, Wakamatu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
Takahisa
T
Anada
Hideshi
H
Matsunaga
Ryouji
R
Karinaga
Kazuya
K
Koumoto
Masami
M
Mizu
Koji
K
Nakano
Seiji
S
Shinkai
Kazuo
K
Sakurai
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004247:DNA / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D008969:Molecular Sequence Data; D011061:Poly A / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012566:Sizofiran / Q000737:chemistry*; D013997:Time Factors
D011134:Polysaccharides; D011061:Poly A; C015465:poly(dA); D012566:Sizofiran; D004247:DNA