Reduced metaphase I associations between the short arms of homologous chromosomes in a Hordeum vulgare L. x H. bulbosum L. diploid hybrid influences the frequency of recombinant progeny.
Hordeum vulgare (cultivated barley) x H. bulbosum (bulbous barley grass) hybrids have been used to obtain disease-resistant recombinant lines (RLs). The RLs contain chromatin transferred from the wild species mostly onto the long arms of recipient barley chromosomes. To determine whether differences in meiotic metaphase I (MI) associations between the long and short arms of homologous chromosomes can account for the preponderance of introgressions on the long arms, we carried out fluorescent in situ hybridisation on MI chromosome preparations obtained from pollen mother cells of a diploid interspecific hybrid. By using various probes, we established that MI associations between the long arms occurred more frequently than between the short arms for the five chromosomes tested.
Pickering
R A
RA
New Zealand Institute for Crop and Food Research Limited, Private Bag 4704, Christchurch, New Zealand. pickeringr@crop.cri.nz
Hudakova
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S
Houben
A
A
Johnston
P A
PA
Butler
R C
RC
D023902:Chromosome Pairing / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D004171:Diploidy; D001467:Hordeum / Q000235:genetics*; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*