Human liver specific transcriptional factor TCP10L binds to MAD4.
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.
Jiang
Dao-Jun
DJ
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, P.R. China.
Yu
Hong-Xiu
HX
Hexige
Sa-Yin
SY
Guo
Ze-Kun
ZK
Wang
Xiang
X
Ma
Li-Jie
LJ
Chen
Zheng
Z
Zhao
Shou-Yuan
SY
Yu
Long
L
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002460:Cell Line; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D056427:t-Complex Genome Region
D051792:Basic Helix-Loop-Helix Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C484347:MXD4 protein, human