Determination of CFTR chloride channel activity and pharmacology using radiotracer flux methods.
Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Here, we described three different protocols to study the properties, regulation and pharmacology of the CFTR Cl- channel in populations of cells and artificial vesicles. These techniques are widely used to evaluate the function of wild-type and mutant CFTR prior to detailed analyses using the patch-clamp technique. Moreover, they have proved especially valuable in the search for new drugs to treat cystic fibrosis.
Norez
Caroline
C
Institut de Physiologie et Biologie Cellulaires CNRS UMR 6187, Université de Poitiers, 40 Avenue du Recteur Pineau, Poitiers, 86022 France.
Heda
Ghanshyam D
GD
Jensen
Timothy
T
Kogan
Ilana
I
Hughes
Lauren K
LK
Auzanneau
Céline
C
Dérand
Renaud
R
Bulteau-Pignoux
Laurence
L
Li
Canhui
C
Ramjeesingh
Mohabir
M
Li
Hongyu
H
Sheppard
David N
DN
Bear
Christine E
CE
Riordan
John R
JR
Becq
Frédéric
F
D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects / Q000378:metabolism*; D003584:Cytological Techniques; D006801:Humans; D017136:Ion Transport / Q000502:physiology*; D017736:Ion-Selective Electrodes; D008081:Liposomes / Q000494:pharmacology; D011868:Radioisotopes / Q000494:pharmacology
C505032:CFTR protein, human; D008081:Liposomes; D011868:Radioisotopes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator