[Association of CTLA-4 promoter -1722 polymorphism with systemic lupus erythematosus in Chinese].
OBJECTIVE
To investigate the possible association of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) promoter -1722 polymorphism with systemic lupus erythematosus (SLE) in the population in southern China.


METHODS
A total of 103 SLE patients (13 males and 90 females with an average age of 33.63+/-12.58 years) diagnosed according to the SLE diagnostic criteria of the American College of Rheumatology revised in 1982 and 110 healthy ethnically matched controls (21 males and 89 females with an average age of 27.49+/-8.60 years), all from southern China, were enrolled in the study. DNA was extracted from EDTA-treated blood samples according to the standard isolation procedure. The restriction fragment length polymorphism-polymerase chain reaction was used to analyze CTLA-4 promoter-1722 polymorphism in SLE patients and healthy controls.


RESULTS
Compared with the controls, the SLE patients had higher frequencies of TC genotype (42% vs 58%, P<0.05) and lower frequency of CC genotype (25% vs 15%, P<0.05). There were no significant differences in the frequencies of TT genotype, alleles and phenotypes between SLE patients and controls; however, significant differences in the frequencies of TT genotype and alleles of CTLA-4 promoter -1722 were found among different races (P<0.05).


CONCLUSIONS
CTLA-4 promoter -1722 polymorphism appears to be associated with SLE susceptibility in southern Chinese population.
Department of Nephrology, Second Hospital of Sun Yat-sen University, Guangzhou 510120, China. anxpu_gzb@21cn.net
An-ping
AP
Xu
Pei-da
PD
Yin
Xiao-yan
XY
Su
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics*; D044466:Asian People; D060908:CTLA-4 Antigen; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human