Effects of MPSS and a potent iNOS inhibitor on traumatic spinal cord injury.
ONO-1714, a selective inhibitor of inducible nitric oxide synthetase (iNOS) attenuated the increase of apoptosis and improved the functional outcome of urinary bladder after traumatic spinal cord injury. These findings suggest that iNOS plays a role in the process of SCI. Early treatment with 30 mg/kg methylprednisolone sodium succinate (MPSS) could also inhibit the expression of iNOS gene, apoptosis and the loss of urinary bladder function. We confirmed that early MPSS treatment may prevent injury associated with apoptosis and urinary bladder disability by reducing iNOS mRNA. However, delayed single MPSS treatment 8 h after spinal cord injury was not effective. Early repeated MPSS treatment might allow greater recovery from acute spinal cord injury.
Department of Orthopedic Surgery, Nagoya University School of Medicine, 466-8550 Nagoya; Nagoya Kyoritsu Hospital, 454-0933 Nagoya, Japan. yuyimin@med.nagoya-u.ac.jp
Yimin
Y
Yu
Yukihiro
Y
Matsuyama
Shojiro
S
Nakashima
Makoto
M
Yanase
Kazutoshi
K
Kiuchi
Naoki
N
Ishiguro
D000578:Amidines / Q000627:therapeutic use*; D000818:Animals; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects; D006574:Heterocyclic Compounds, 2-Ring / Q000627:therapeutic use*; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D008297:Male; D008776:Methylprednisolone Hemisuccinate / Q000627:therapeutic use*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013119:Spinal Cord Injuries / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology; D013997:Time Factors; D001750:Urinary Bladder, Neurogenic / Q000188:drug therapy / Q000209:etiology
C407360:7-chloro-3-imino-5-methyl-2-azabicyclo(4.1.0)heptane; D000578:Amidines; D006574:Heterocyclic Compounds, 2-Ring; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D008776:Methylprednisolone Hemisuccinate; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat