PCR detection of hemolysin (vhh) gene in Vibrio harveyi.
The Vibrio harveyi hemolysin gene (vhh), which encodes for a virulence factor involved in pathogenicity to fish and shellfish species, may be targeted for species detection or strain differentiation. Primers designed for this gene were used in detection studies of V. harveyi strains from various hosts. One primer set among four tested, could amplify the expected gene fragment in PCR using templates from all 11 V. harveyi strains studied. Detection of the presence of the hemolysin gene could therefore serve as a suitable detection marker of Vibrio harveyi isolates potentially pathogenic to fish and shrimps.
National Institute of Molecular Biology and Biotechnology, University of the Philippines, Diliman, Quezon City 1101, Philippines.
Mia Judith U
MJ
Conejero
Cynthia T
CT
Hedreyda
D001483:Base Sequence; D017931:DNA Primers; D006460:Hemolysin Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D017931:DNA Primers; D006460:Hemolysin Proteins