Insemination with isolated and virologically tested spermatozoa is a safe way for human immunodeficiency type 1 virus-serodiscordant couples with an infected male partner to have a child.
OBJECTIVE
To enable serodiscordant couples to reproduce while minimizing the risk of infecting the female partner and to demonstrate the feasibility of including these couples in an insemination program.


METHODS
Prospective follow-up study.


METHODS
Ambulatory IUIs.


METHODS
Fifty-six HIV-1-serodiscordant couples with a male infected partner.


METHODS
Sperm cell separation by the gradient density method followed by the swim-up method, virological detection, and IUI after ovarian stimulation.


METHODS
Pregnancy rate, HIV-1 test, and p24 antigenemia in women.


RESULTS
Two hundred thirteen IUIs were performed, and 37 pregnancies occurred, resulting in 33 births and 2 ongoing pregnancies. Fifty percent of couples have a child. No women were infected.


CONCLUSIONS
Our study demonstrates the efficiency of an IUI program with prepared and virologically tested spermatozoa in serodiscordant couples with an HIV-1-infected male partner, allowing the couples to have children without transmission of the virus to the female partner.
Bujan
Louis
L
CECOS Midi-Pyrénées and Research Group on Human Fertility; Hôpital Paule de Viguier, Toulouse, France. bujan.l@chu-toulouse.fr
Pasquier
Christophe
C
Labeyrie
Evelyne
E
Lanusse-Crousse
Patrick
P
Morucci
Mireille
M
Daudin
Myriam
M
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D002499:Centrifugation, Density Gradient; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D016655:HIV Core Protein p24 / Q000097:blood; D015658:HIV Infections / Q000188:drug therapy / Q000517:prevention & control / Q000821:virology*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000821:virology*
D015483:HIV Antibodies; D016655:HIV Core Protein p24; D012367:RNA, Viral