Deficient eosinophil chemotaxis-promoting activity of genetically normal mast cells transplanted into subcutaneous tissue of Mitfmi-vga9/Mitfmi-vga9 mice: comparison of the activity and mast cell distribution pattern with KitW/KitW-vMice.
Despite the practical lack of mast cells in the skin tissue of WBB6F(1)-Kit(W)/Kit(W-v), the skin tissue of WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice contains one third of mast cells than that of WBB6F(1)-+/+ mice. We attempted to investigate the function of the decreased but appreciable number of mast cells in the skin of WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice. The substance P (SP)-induced eosinophil infiltration was examined using air-bleb assay. The air-bleb membrane was composed of the subcutaneous connective tissue. Unexpectedly, we found that the air-bleb membranes formed in the back of WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice contained no mast cells. The WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice showed impaired SP-induced eosinophil infiltration as observed in WBB6F(1)-Kit(W)/Kit(W-v) mice, indicating that mast cells detected in the dermis of WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice did not help SP-induced eosinophil infiltration. Subcutaneous transplantation of cultured mast cells from WBB6F(1)-+/+ mice normalized SP-induced eosinophil infiltration in WBB6F(1)-Kit(W)/Kit(W-v) mice but not in WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice. The greater number and the more dispersed distribution pattern of mast cells that appeared in the subcutaneous connective tissue of WBB6F(1)-Kit(W)/Kit(W-v) mice after the transplantation appeared to explain the difference between WBB6F(1)-Kit(W)/Kit(W-v) and WBB6F(1)-Mitf(mi-vga9)/Mitf(mi-vga9) mice.
Oboki
Keisuke
K
Department of Pathology (Room C2), Graduate School of Frontier Bioscience and Medical School, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.
Morii
Eiichi
E
Kitamura
Yukihiko
Y
D000818:Animals; D017690:Cell Transplantation; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology*; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000276:immunology*; D040521:Subcutaneous Tissue / Q000276:immunology / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology
D013373:Substance P