Developmental stage-dependent modulation of synapses by postsynaptic expression of activated calcium/calmodulin-dependent protein kinase II.
In Drosophila neuromuscular junctions, there is a unique system which consists of two neighboring muscles (M6 and M7) innervated by the same neurons and a gene of interest can be expressed in only M6 or in both muscles by GAL4-upstream activating sequence expression system. By using this system, we previously demonstrated that expression of activated calcium/calmodulin-dependent protein kinase II (CaMKII) in the muscle cell promotes coordinated maturation of pre- and postsynaptic sites of larvae just after hatching (JAH larvae) in a synapse-specific manner. Here we show that the promotive effects are no longer seen in the older larvae, 8-10 h after hatching (8 h AH larvae). Morphological studies indicate that CaMKII activation in fact reduces postsynaptic sites at 8 h AH. This is opposite to the effect observed in JAH larvae. These results suggest that the mode of CaMKII function switches during development, and that regulation of postsynaptic CaMKII activity is necessary for normal synaptic development. Finally, we report that in 8 h AH but not in JAH larvae, synapses on M7, in which CaMKII activity is not manipulated, are affected by the expression of activated CaMKII in M6. This suggests the interesting possibility that at certain developmental stages only, modification of synapses on one target cell can influence the synapses on another target cell innervated by the same neurons.
Morimoto-Tanifuji
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Department of Physics, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyoku, Tokyo 113-0033, Japan.
Kazama
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Nose
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D000375:Aging / Q000378:metabolism / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D004330:Drosophila / Q000196:embryology / Q000502:physiology; D004625:Embryo, Nonmammalian; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006735:Horseradish Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007814:Larva; D008954:Models, Biological; D009132:Muscles / Q000196:embryology* / Q000502:physiology; D009469:Neuromuscular Junction / Q000196:embryology*; D017729:Presynaptic Terminals / Q000502:physiology; D018091:Receptors, AMPA / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*; D013997:Time Factors
D018091:Receptors, AMPA; D006735:Horseradish Peroxidase; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C104722:glutamate receptor ionotropic, AMPA 2