[Development of monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic assay for lasalocid and semduramicin].
Monoclonal antibodies (MAbs) against lasalocid and semduramicin were prepared using keyhole limpet hemocyanin conjugates for the immunization of mice. With these MAbs, we developed quantitative enzyme-linked immunosorbent assay (ELISA) methods for lasalocid and semduramicin. The ELISAs were quantitative in the ranges of 0.1-50 ng/mL for lasalocid and 0.05-12.5 ng/mL for semduramicin, and showed 50% inhibition concentrations of 1.2 ng/mL for lasalocid and 0.5 ng/mL for semduramicin. The coefficient of variations (CV%) of lasalocid were 0.3-4.4% for intra-assay and 0.5-5.1% for inter-assay and those of semduramicin were 0.1-4.6% for intra-assay and 0.3-5.2% for inter-assay. The detection limits for lasalocid and semduramicin were 10 ng/g and 5 ng/g in chicken liver and muscle, respectively. Based on the immunochromatographic method, rapid test kits for lasalocid and semduramicin were also developed. With these kits, the detection limits of lasalocid were 50 ng/mL for standard solution and 125 ng/g for chicken muscle, and those of semduramicin were 10 ng/mL for standard solution and 100 ng/g for chicken muscle.
Research Institute for Animal Science in Biochemistry and Toxicology, 3-7-11, Hashimotodai, Sagamihara, Kanagawa 229-1132, Japan.
Hiroo
H
Watanabe
Atsuko
A
Satake
Yasumasa
Y
Kido
Akio
A
Tsuji
D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D002645:Chickens; D002845:Chromatography / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis* / Q000276:immunology; D004350:Drug Residues / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D007832:Lasalocid / Q000032:analysis* / Q000276:immunology; D008460:Meat / Q000032:analysis*; D051379:Mice; D009550:Nigericin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D003049:Coccidiostats; D011933:Reagent Kits, Diagnostic; C072941:semduramicin; D009550:Nigericin; D007832:Lasalocid