The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism.
Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for approximately 20% of global carbon fixation. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand-base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.
Armbrust
E Virginia
EV
School of Oceanography, University of Washington, Seattle, WA 98195, USA. armbrust@ocean.washington.edu
Berges
John A
JA
Bowler
Chris
C
Green
Beverley R
BR
Martinez
Diego
D
Putnam
Nicholas H
NH
Zhou
Shiguo
S
Allen
Andrew E
AE
Apt
Kirk E
KE
Bechner
Michael
M
Brzezinski
Mark A
MA
Chaal
Balbir K
BK
Chiovitti
Anthony
A
Davis
Aubrey K
AK
Demarest
Mark S
MS
Detter
J Chris
JC
Glavina
Tijana
T
Goodstein
David
D
Hadi
Masood Z
MZ
Hellsten
Uffe
U
Hildebrand
Mark
M
Jenkins
Bethany D
BD
Jurka
Jerzy
J
Kapitonov
Vladimir V
VV
Kröger
Nils
N
Lau
Winnie W Y
WW
Lane
Todd W
TW
Larimer
Frank W
FW
Lippmeier
J Casey
JC
Lucas
Susan
S
Medina
Mónica
M
Montsant
Anton
A
Obornik
Miroslav
M
Parker
Micaela Schnitzler
MS
Palenik
Brian
B
Pazour
Gregory J
GJ
Richardson
Paul M
PM
Rynearson
Tatiana A
TA
Saito
Mak A
MA
Schwartz
David C
DC
Thamatrakoln
Kimberlee
K
Valentin
Klaus
K
Vardi
Assaf
A
Wilkerson
Frances P
FP
Rokhsar
Daniel S
DS
D000222:Adaptation, Physiological; D020418:Algal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D000818:Animals; D005075:Biological Evolution; D002467:Cell Nucleus / Q000235:genetics; D002875:Chromosomes; D004247:DNA / Q000235:genetics; D017377:Diatoms / Q000737:chemistry / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D017753:Ecosystem; D004734:Energy Metabolism; D016678:Genome; D007501:Iron / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010788:Photosynthesis; D018087:Plastids / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012824:Silicic Acid / Q000378:metabolism; D013559:Symbiosis; D014508:Urea / Q000378:metabolism
D020418:Algal Proteins; D045342:Light-Harvesting Protein Complexes; D012824:Silicic Acid; D014508:Urea; D004247:DNA; D007501:Iron; D009584:Nitrogen