Transfection microarray of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified glass slide.
Cell-based microarrays are emerging as a tool for analyzing the functions of genes in cells. However, partly due to the difficulty of cell immobilization, the application of this method has been limited to adherent cells. We previously reported a method that rapidly and strongly attached living nonadherent cells to glass slides modified with a cell membrane anchoring reagent, designated a biocompatible anchor for membrane (BAM). Here we demonstrate that plasmid DNA deposited in a defined area on BAM-modified glass slides was transfected into nonadherent K562 cells immobilized on the DNA-deposited and BAM-modified slides. This method allowed the transfection of K562 cells not only with plasmid cDNA expression vectors but also with small interfering RNA (siRNA) at a defined location on the BAM-modified slides. We expect this methodology to greatly expand the scope of current cell microarray technology.
National Institute of Advanced Industrial Science and Technology, Amagasaki, Japan.
Koichi
K
Kato
Kohei
K
Umezawa
Masato
M
Miyake
Jun
J
Miyake
Teruyuki
T
Nagamune
D001672:Biocompatible Materials / Q000737:chemistry*; D018914:Cells, Immobilized; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004986:Ether / Q000737:chemistry; D015870:Gene Expression; D005898:Glass; D006801:Humans; D007202:Indicators and Reagents; D020014:K562 Cells; D008055:Lipids; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering; D013499:Surface Properties; D014162:Transfection
D001672:Biocompatible Materials; D018076:DNA, Complementary; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D008567:Membranes, Artificial; D034741:RNA, Small Interfering; D004986:Ether; D011092:Polyethylene Glycols