Analysis of interactions between regulator of G-protein signaling-14 and microtubules.
Microtubules are dynamic polymers essential for mitosis and cell division, intracellular transport, and maintaining cell organization and structure. Microtubule dynamics are tightly controlled in a context-specific manner by a myriad of microtubule-associated proteins. We have identified regulator of G-protein signaling-14 (RGS14) as a microtubule-associated protein. RGS14 is a component of the mitotic apparatus that binds directly to and stabilizes microtubules in vitro and is essential for the first cell division in the mouse embryo. This article describes methods used for examining the impact of the RGS14/microtubule interaction in vivo and in vitro.
Department of Physiology and Pharmacology, London, Ontario, Canada.
Luke
L
Martin-McCaffrey
Francis S
FS
Willard
Agnieszka
A
Pajak
Lina
L
Dagnino
David P
DP
Siderovski
Sudhir J A
SJ
D'Souza
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D020710:RGS Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D020710:RGS Proteins; D011993:Recombinant Fusion Proteins; C491399:Rgs14 protein, mouse; D014404:Tubulin