Optimisation of the derivatisation reaction and subsequent headspace solid-phase microextraction method for the direct determination of chlorophenols in red wine.
An acetylation reaction for the derivatisation of the three chlorophenols involved in cork taint was optimised using a Doehlert design for direct application in wine samples. In this first step, the optimum reaction pH, by adding different amounts of KHCO3, and the required quantity of derivatisation reagent were fixed. Then a series of parameters relevant for the headspace solid-phase microextraction process, such as desorption conditions, salt addition and agitation sample were evaluated. A simultaneous study of the type of fibre and extraction temperature was performed at five levels and based on the results obtained the rest of factors (sample volume and exposition time) that could potentially affect the extraction yields were optimised by a central composite design. According to the validation of the method, we propose here, to our knowledge, the first application of solid-phase microextraction for the direct analysis of chlorophenols in red wine samples.
Department of Chemistry, University of La Rioja, C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
Almudena
A
Martínez-Uruñuela
José María
JM
González-Sáiz
Consuelo
C
Pizarro
D000089:Acetic Anhydrides / Q000737:chemistry; D001639:Bicarbonates / Q000737:chemistry; D002733:Chlorophenols / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D017680:Potassium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014920:Wine / Q000032:analysis*
D000089:Acetic Anhydrides; D001639:Bicarbonates; D002733:Chlorophenols; D017680:Potassium Compounds; D012492:Salts; C031800:acetic anhydride; C026329:potassium bicarbonate