Comparison of six density gradient media for selection of cryopreserved donor spermatozoa.
The aim of our study was to evaluate the efficiency of 4 density gradient media for motile cryopreserved spermatozoa selection to Percoll (Kabi Pharmacia, Uppsala, Sweden) and to Puresperm (J.C.D. International Laboratory, L'Aigle, France). Puresperm was the new medium chosen in our laboratory in 1996 as the substitute for Percoll. The solutions tested were 3 colloidal silane-coated silica particle media (Isolate, SpermGrad-100, Sil-Select Plus) and iodixanol (Optiprep). Semen parameters analyzed after selection were concentration, motility, and morphology. Semen parameters after Puresperm gradient had similar values compared to Percoll. Optiprep was less efficient with a poor concentration. Isolate had a comparatively better concentration, but the capacity of selection was not satisfactory. SpermGrad-100 and Sil-Select Plus were less effective than Puresperm. In conclusion, Puresperm could be considered a better alternative to Percoll for cryopreserved spermatozoa migration.
Laboratoire de Biologie de la Reproduction, Centre Hospitalier Universitaire-Charles Nicolle, 1 rue de Germont, 76031 Rouen cedex, France.
Nathalie
N
Mousset-Siméon
Nathalie
N
Rives
Lydie
L
Masse
Florence
F
Chevallier
Bertrand
B
Mace
D002499:Centrifugation, Density Gradient / Q000379:methods* / Q000592:standards; D015925:Cryopreservation; D006801:Humans; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D012996:Solutions; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa; D014283:Triiodobenzoic Acids
D012996:Solutions; D014283:Triiodobenzoic Acids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; C044834:iodixanol