ZAK re-programs atrial natriuretic factor expression and induces hypertrophic growth in H9c2 cardiomyoblast cells.
Various intracellular or intercellular stimuli have been associated with the development of cardiac cell hypertrophy. However, the mechanisms underlying this association are not completely understood. In a previous study we determined that ZAK mRNA expression is abundant in heart. ZAK is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. We, therefore, investigated the potential involvement of ZAK (which in cultured H9c2 cardiomyoblast cell is a positive mediator of cell hypertrophy). Our results showed that the expression of a wild-type form of ZAK induces the characteristic hypertrophic growth features, including increased cell size, elevated atrial natriuretic factor expression, and increased actin fiber organization.
Institute of Biochemistry, Chung-Shan Medical University, Taichung 402, Taiwan.
Chih-Yang
CY
Huang
Pin Ju
PJ
Chueh
Chien-Tang
CT
Tseng
Kuan-Yu
KY
Liu
Hsiao-Ya
HY
Tsai
Wei-Wen
WW
Kuo
Ming-Yung
MY
Chou
Jaw-Ji
JJ
Yang
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D020816:Amino Acid Motifs; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis*; D015153:Blotting, Western; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004220:Disulfides / Q000737:chemistry; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D047468:Immunoprecipitation; D048031:JNK Mitogen-Activated Protein Kinases / Q000737:chemistry; D007930:Leucine / Q000737:chemistry; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System; D009154:Mutation; D032383:Myocytes, Cardiac / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary; D051381:Rats; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
D000199:Actins; C493956:CDKN1A protein, human; C493958:Cdkn1a protein, rat; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004220:Disulfides; D016328:NF-kappa B; D009320:Atrial Natriuretic Factor; D011494:Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020930:MAP Kinase Kinase Kinases; C413047:MAP3K20 protein, human; D007930:Leucine