A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli.
Apurinic/apyrimidinic (AP) sites are alkali labile lesions that, when encountered during DNA replication, can block polymerases or potentially result in mutagenic events. Owing to the instability of 2-deoxyribose lesions (AP), a chemically stable tetrahydrofuran analog (F) is often used as a model of abasic sites. A comparison of the two lesions in Saccharomyces cerevisiae revealed that the model lesion and 2-deoxyribose have distinct in vivo effects. Comprehensive comparative analyses of F and AP have not been carried out in Escherichia coli. We conducted a side-by-side investigation of F and AP in E.coli to compare their biological effects and interactions with SOS polymerases. Both lesions were examined in SOS-induced and uninduced cells. Our studies reveal that in uninduced E.coli the effects of individual polymerases in the replication of plasmids containing F or AP are distinct. However, when cells are SOS-induced, the biological effects of F and AP are similar.
Department of Chemistry, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
Kelly M
KM
Kroeger
Myron F
MF
Goodman
Marc M
MM
Greenberg
D004249:DNA Damage; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003855:Deoxyribose / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005663:Furans / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism; D010957:Plasmids / Q000096:biosynthesis / Q000737:chemistry; D013014:SOS Response, Genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004269:DNA, Bacterial; D005663:Furans; D009711:Nucleotides; C018674:tetrahydrofuran; D003855:Deoxyribose; D004259:DNA-Directed DNA Polymerase