Impact and efficiency of GH10 and GH11 thermostable endoxylanases on wheat bran and alkali-extractable arabinoxylans.
The results of a comparative study of two thermostable (1-->4)-beta-xylan endoxylanases using a multi-technical approach indicate that a GH11 xylanase is more useful than a GH10 xylanase for the upgrading of wheat bran into soluble oligosaccharides. Both enzymes liberated complex mixtures of xylooligosaccharides. 13C NMR analysis provided evidence that xylanases cause the co-solubilisation of beta-glucan, which is a result of cell-wall disassembly. The simultaneous use of both xylanases did not result in a synergistic action on wheat bran arabinoxylans, but instead led to the production of a product mixture whose profile resembled that produced by the action of the GH10 xylanase alone. Upon treatment with either xylanase, the diferulic acid levels in residual bran were unaltered, whereas content in ferulic and p-coumaric acids were unequally decreased. With regard to the major differences between the enzymes, the products resulting from the action of the GH10 xylanase were smaller in size than those produced by the GH11 xylanase, indicating a higher proportion of cleavage sites for the GH10 xylanase. The comparison of the kinetic parameters of each xylanase using various alkali-extractable arabinoxylans indicated that the GH10 xylanase was most active on soluble arabinoxylans. In contrast, probably because GH11 xylanase can better penetrate the cell-wall network, this enzyme was more efficient than the GH10 xylanase in the hydrolysis of wheat bran. Indeed the former enzyme displayed a nearly 2-fold higher affinity and a 6.8-fold higher turnover rate in the presence of this important by-product of the milling industry.
Beaugrand
Johnny
J
Institut National de la Recherche Agronomique, UMR FARE-614, Centre de Recherche Agronomique, 2 esplanade Roland Garros, BP 224, F-51686 Reims, France.
Chambat
Gérard
G
Wong
Vicky W K
VW
Goubet
Florence
F
Rémond
Caroline
C
Paës
Gabriel
G
Benamrouche
Samina
S
Debeire
Philippe
P
O'Donohue
Michael
M
Chabbert
Brigitte
B
D004043:Dietary Fiber / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D007700:Kinetics; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D014990:Xylans / Q000378:metabolism*; D014994:Xylose
D004043:Dietary Fiber; D009844:Oligosaccharides; D014990:Xylans; C085118:arabinoxylan; D014994:Xylose; D043364:Endo-1,4-beta Xylanases