[ER-alpha, IGF-1R expressions and co-expressions in newborn rats with experimental hypoxic-ischemic brain damage].
OBJECTIVE
To observe the distribution and change of insulin-like growth factor-1 receptor (IGF-1R), estrogen receptor-alpha (ER-alpha) and their co-expression in the brains of normal newborn female rats and in the brains of those with hypoxic-ischemic brain damage (HIBD).


METHODS
The 7 day-old female SD rats were subjected to the ligation of right carotid artery (ischemia), then they were put into a box full with 8% oxygen and 92% nitrogen for an hour (hypoxia). The methods of immunohistochemistry and double-immunohistochemistry were used to detect the expression of IGF-1R, ER-alpha and their co-expression in the brain.


RESULTS
(1) There were extensive immunoreactions of IGF-IR, ER-alpha in newborn rat's brain, and there were extensive co-localization of IGF-1R and ER-alpha in the same cells of cerebral cortex. (2) There were no changes in the expressions of IGF-1R and ER-alpha, no changes in the co-expression of IGF-1R and ER-alpha at 24 hours of HIBD; whereas there were increasing expressions of IGF-1R, ER-alpha and increasing co-expression of IGF-1R, ER-alpha in the cerebral cortex on the 7th day of HIBD.


CONCLUSIONS
The above results suggest that there are co-action of IGF-1R and ER-alpha in the repair of hypoxic-ischemic brain damage.
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Bin
B
Xia
Yu-Jia
YJ
Yao
Juan
J
Chen
Kai-jian
KJ
Cheng
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis* / Q000235:genetics
D047628:Estrogen Receptor alpha; D017526:Receptor, IGF Type 1