Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis.
PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.
School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS. r.j.cox@bris.ac.uk.
Russell J
RJ
Cox
Frank
F
Glod
Deirdre
D
Hurley
Colin M
CM
Lazarus
Thomas P
TP
Nicholson
Brian A M
BA
Rudd
Thomas J
TJ
Simpson
Barrie
B
Wilkinson
Ying
Y
Zhang
D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005784:Gene Amplification; D019151:Peptide Library; D048630:Polyketide Synthases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014233:Tricarboxylic Acids / Q000378:metabolism*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D018076:DNA, Complementary; D019151:Peptide Library; D014233:Tricarboxylic Acids; C075117:squalestatin 1; D048630:Polyketide Synthases