Classification of Giardia duodenalis parasites in Turkey into groups A and B using restriction fragment length polymorphism.
PCR-RFLP (restriction fragment length polymorphism) analysis was used to determine the relation of Giardia duodenalis Groups A and B. Of these, 17 (85%) were found as Group A in symptomatic cases; 22 (92%) were Group B in asymptomatic cases by using PCR-RFLP (p < 0.001). Interestingly, 5 (83%) were Group A in examination of endoscopy aspirates of symptomatic cases, as 5 (83%) were Group B in asymptomatic cases.
Aydin
Ahmet F
AF
Department of Infectious Diseases and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey.
Besirbellioglu
Bülent A
BA
Avci
Ismail Y
IY
Tanyuksel
Mehmet
M
Araz
Engin
E
Pahsa
Alaaddin
A
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D003967:Diarrhea / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005260:Female; D005872:Giardia / Q000145:classification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011336:Probability; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D014421:Turkey
D016054:DNA, Protozoan