L-carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts.
Telomere is the repetitive DNA sequence at the end of chromosomes, which shortens progressively with cell division and limits the replicative potential of normal human somatic cells. L-carnosine, a naturally occurring dipeptide, has been reported to delay the replicative senescence, and extend the lifespan of cultured human diploid fibroblasts. In this work, we studied the effect of carnosine on the telomeric DNA of cultured human fetal lung fibroblast cells. Cells continuously grown in 20 mM carnosine exhibited a slower telomere shortening rate and extended lifespan in population doublings. When kept in a long-term nonproliferating state, they accumulated much less damages in the telomeric DNA when cultured in the presence of carnosine. We suggest that the reduction in telomere shortening rate and damages in telomeric DNA made an important contribution to the life-extension effect of carnosine.
Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Lan
L
Shao
Qing-Huan
QH
Li
Zheng
Z
Tan
D000375:Aging; D015139:Blotting, Southern; D002336:Carnosine / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004171:Diploidy; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D017382:Reactive Oxygen Species; D012685:Sepharose / Q000737:chemistry; D016615:Telomere / Q000648:ultrastructure*; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D017382:Reactive Oxygen Species; D002336:Carnosine; D004247:DNA; D012685:Sepharose; D001616:beta-Galactosidase