Ureotelism is inducible in the neotropical freshwater Hoplias malabaricus (Teleostei, Erythrinidae).
Increased environmental pH decreases ammonia transport through the gills, impairing nitrogenous waste. The consequent toxicity is usually drastic to most fishes. A few species are able to synthesize urea as a way to detoxify plasma ammonia. We studied three teleosts of the family Erythrinidae living in distinct environments, and assumed the biochemical behaviors would be different in spite of their being closely related species. Adult fish collected in the wild were submitted to alkaline water and the urea excretion rate was determined. The specific activity of urea cycle enzymes was determined in liver samples of fish from neutral waters. The studied species Hoplias lacerdae, Hoplerithrynus unitaeniatus, and Hoplias malabaricus are ureogenic. Urea synthesis is not a metabolic way to detoxify ammonia in H. lacerdae and Hoplerithrynus unitaeniatus exposed to an alkaline environment. The plasma ammonia profile of both species showed two distinct biochemical responses. Urea excretion of H. malabaricus was high in alkaline water, and the transition to ureotelism is proposed. The nitrogen excretion rate of H. malabaricus was among the highest values reported and the high urea excretion leads us to include this species as ureotelic in alkaline water.
Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, Rod. Washington Luís, km 235, C.P. 676, CEP 13560-905, São Carlos, SP, Brazil. gil@power.ufscar.br
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D000222:Adaptation, Physiological; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation; D004798:Enzymes / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism*; D014508:Urea / Q000378:metabolism*
D004798:Enzymes; D000641:Ammonia; D014508:Urea; D009584:Nitrogen