Study of the myenteric and submucous plexuses after BAC treatment in the intestine of rats.
A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI--ileum control; CBI--denervated ileum; SC--colon control; and CBC--denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2. The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric plexus there was also a significant decrease in the number of ganglia/mm2 (13.8% in group CBI and 52.14% in group CBC) and in the number of neurons/ganglion (33.9% in group CBI and 39.6% in group CBC). The morphological analyses showed that there was an alteration in the shape of the ganglia of the ileal and colonic myenteric plexus. The area of the cell bodies had a significant increase both in the myenteric and the submucous plexus in groups CBI and CBC. These data demonstrate that the BAC treatment causes morphologic and quantitative changes in the myenteric plexus and quantitative changes in the cell body area of the submucous plexus.
Department of Morphophysiological Sciences, Universidade Estadual de Maringá, PR, Brazil. ncbuttow@uem.br
Nilza Cristina
NC
Buttow
Miriam
M
Santin
Luciana Conci
LC
Macedo
Aline Cristina
AC
Neres Teixeira
Gisele Caroline
GC
Novakowski
Taíse Roberta
TR
Bolonheis Armelin
Kathya
K
Assmann
D000818:Animals; D001548:Benzalkonium Compounds / Q000494:pharmacology*; D003106:Colon / Q000166:cytology* / Q000294:innervation / Q000378:metabolism; D003714:Denervation / Q000379:methods; D005724:Ganglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007082:Ileum / Q000166:cytology* / Q000294:innervation / Q000378:metabolism; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013368:Submucous Plexus / Q000166:cytology* / Q000378:metabolism
D001548:Benzalkonium Compounds