[Detection of antibiotic contents in cerebrospinal fluid].
The suggested method provides for the semi-quantitative detection of antibiotics in liquor made for the purpose of prognosticating the possibility of cultural secretion of infectious agents of purulent bacterial meningitis (PBM); it is also a basis for the need in using the non-cultural diagnostic tools. The method can be used in detecting the pharmacokinetic specificity of antibacterial drugs now in use and of their combinations with the aim of making corrections in antibiotic therapy. It is based on inhibiting the surface growth of test-culture by a liquor-dissolved antibiotic with the growth-delay zone being shaped. The preparation concentration is estimated in U/ml of the penicillin equivalent (PE). Examinations of 131 liquor samples (primary puncture) obtained from patients with meningitis showed clear-cut correlations between the antibiotics' concentrations, cultural verification of the PBM diagnosis and the efficiency of non-cultural examinations. 82.14% of cultures were derived from liquor samples with the negative antibacterial activity (range 1); 7.14% of cultures were derived from liquor samples containing the antibacterial preparation of up to 0.5 U/ml PE (range 2); culture of secondary PBM agents (S. aureus) were isolated from liquor samples containing up to 10 U/ml PE (range 3); and no microbe agents were isolated from liquor samples containing above 10 U/ml PE (range 4). The designed method, when used, demonstrated a high efficiency and significance of laboratory PBM diagnosis in presence of the antibacterial liquor efficiency.
Beloshitskiĭ
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Koroleva
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D000900:Anti-Bacterial Agents / Q000134:cerebrospinal fluid* / Q000494:pharmacology; D015169:Colony Count, Microbial; D006801:Humans; D007841:Latex Fixation Tests; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents