Liquid chromatography/tandem mass spectrometry of unusual phenols from Yucca schidigera bark: comparison with other analytical techniques.
Qualitative and quantitative analyses of phenolic compounds are of interest for both medicinal and food plants. In the present work, the phenolic fraction from Yucca schidigera, a plant bearing the GRAS (Generally Recognized as Safe) label approved by the US Food and Drug Administration, was studied. Crude extracts of Y. schidigera bark were investigated by liquid chromatography/UV spectrophotometry with diode-array detection, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS), in order to develop and optimize simple and rapid techniques to determine both stilbenes and yuccaols for the purposes of quality control of collected material. With optimal LC and MS conditions, stilbenes and yuccaols were quantified with all the proposed methods and the results were compared. Sensitivity was evaluated and the results indicated that MS/MS detection in the multiple reaction monitoring mode is easily applicable to this plant and allows the rapid and direct identification and quantification of these peculiar compounds in crude plant extracts.
Dipartimento di Scienze Farmaceutiche, Universitá di Salerno via Ponte don Melillo, 84084 Fisciano (SA), Italy.
Paola
P
Montoro
Sonia
S
Piacente
Wieslaw
W
Oleszek
Cosimo
C
Pizza
D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013141:Spiro Compounds / Q000032:analysis / Q000737:chemistry; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry; D027623:Yucca / Q000737:chemistry*
D010636:Phenols; D010936:Plant Extracts; D013141:Spiro Compounds; D013267:Stilbenes; C488887:yuccaol C; C487250:yuccaol D