Functional evolution of the vertebrate Myb gene family: B-Myb, but neither A-Myb nor c-Myb, complements Drosophila Myb in hemocytes.
The duplication of genes and genomes is believed to be a major force in the evolution of eukaryotic organisms. However, different models have been presented about how duplicated genes are preserved from elimination by purifying selection. Preservation of one of the gene copies due to rare mutational events that result in a new gene function (neofunctionalization) necessitates that the other gene copy retain its ancestral function. Alternatively, preservation of both gene copies due to rapid divergence of coding and noncoding regions such that neither retains the complete function of the ancestral gene (subfunctionalization) may result in a requirement for both gene copies for organismal survival. The duplication and divergence of the tandemly arrayed homeotic clusters have been studied in considerable detail and have provided evidence in support of the subfunctionalization model. However, the vast majority of duplicated genes are not clustered tandemly, but instead are dispersed in syntenic regions on different chromosomes, most likely as a result of genome-wide duplications and rearrangements. The Myb oncogene family provides an interesting opportunity to study a dispersed multigene family because invertebrates possess a single Myb gene, whereas all vertebrate genomes examined thus far contain three different Myb genes (A-Myb, B-Myb, and c-Myb). A-Myb and c-Myb appear to have arisen by a second round of gene duplication, which was preceded by the acquisition of a transcriptional activation domain in the ancestral A-Myb/c-Myb gene generated from the initial duplication of an ancestral B-Myb-like gene. B-Myb appears to be essential in all dividing cells, whereas A-Myb and c-Myb display tissue-specific requirements during spermatogenesis and hematopoiesis, respectively. We now report that the absence of Drosophila Myb (Dm-Myb) causes a failure of larval hemocyte proliferation and lymph gland development, while Dm-Myb(-/-) hemocytes from mosaic larvae reveal a phagocytosis defect. In addition, we show that vertebrate B-Myb, but neither vertebrate A-Myb nor c-Myb, can complement these hemocyte proliferation defects in Drosophila. Indeed, vertebrate A-Myb and c-Myb cause lethality in the presence or absence of endogenous Dm-Myb. These results are consistent with a neomorphic origin of an ancestral A-Myb/c-Myb gene from a duplicated B-Myb-like gene. In addition, our results suggest that B-Myb and Dm-Myb share essential conserved functions that are required for cell proliferation. Finally, these experiments demonstrate the utility of genetic complementation in Drosophila to explore the functional evolution of duplicated genes in vertebrates.
Davidson
Colin J
CJ
Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Tirouvanziam
Rabindra
R
Herzenberg
Leonard A
LA
Lipsick
Joseph S
JS
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D005075:Biological Evolution; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D020440:Gene Duplication; D005799:Genes, Dominant; D020570:Genes, myb / Q000235:genetics*; D005816:Genetic Complementation Test; D006434:Hemocytes / Q000378:metabolism*; D053613:Janus Kinase 1; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D010587:Phagocytosis; D010802:Phylogeny; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D050794:STAT1 Transcription Factor; D051193:Toll-Like Receptors; D015534:Trans-Activators / Q000378:metabolism; D014714:Vertebrates / Q000502:physiology*
D023181:Antimicrobial Cationic Peptides; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D011956:Receptors, Cell Surface; D050794:STAT1 Transcription Factor; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors; D015534:Trans-Activators; D011505:Protein-Tyrosine Kinases; D053613:Janus Kinase 1