5-(2-Aminoethyl)dithio-2-nitrobenzoate as a more base-stable alternative to Ellman's reagent.
[reaction: see text] 5-(2-Aminoethyl)dithio-2-nitrobenzoate (ADNB) reacts with free thiols with kinetics similar to those of Ellman's reagent but has dramatically improved stability under alkaline conditions, making it an excellent alternative to Ellman's reagent for the quantitation of thiol contents and enzymatic assays under basic pH conditions.
Zhu
Jinge
J
Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
Dhimitruka
Ilirian
I
Pei
Dehua
D
D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry*; D007700:Kinetics; D009579:Nitrobenzoates / Q000737:chemistry*
C508465:5-(2-aminoethyl)dithio-2-nitrobenzoate; D007202:Indicators and Reagents; D009579:Nitrobenzoates