Involvement of the yeast metacaspase Yca1 in ubp10Delta-programmed cell death.
UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function.
Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
Maurizio
M
Bettiga
Luciano
L
Calzari
Ivan
I
Orlandi
Lilia
L
Alberghina
Marina
M
Vai
D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D001205:Ascorbic Acid / Q000502:physiology; D020169:Caspases / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005434:Flow Cytometry; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008856:Microscopy, Fluorescence; D016254:Mutagenesis, Insertional; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D013104:Spheroplasts / Q000502:physiology; D043222:Ubiquitin Thiolesterase
D004271:DNA, Fungal; D009687:Nuclear Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C403282:UBP10 protein, S cerevisiae; D043222:Ubiquitin Thiolesterase; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D001205:Ascorbic Acid