Use of high-performance liquid chromatography to assess airborne mycotoxins. Aflatoxins and ochratoxin A.
An HPLC analytical method combining methanol-deionised water (80:20, v/v) extraction, methanol-acetonitrile (50:50, v/v) extraction and fluorescence detection was implanted to analyse ochratoxin A and aflatoxins B1, B2, G1 and G2 of air samples collected during the usual production process in a number of workplaces of a coffee factory to assess the occupational exposure of the engaged workers. The average levels of airborne ochratoxin A and aflatoxins were less than 1.2 and 0.4 ng/m3, respectively, using 50 L air samples. When 150 L air samples were used, levels lower than 0.04 ng/m3 ochratoxin A and 0.013 ng/m3 for aflatoxins B1, B2, G1 and G2, could be detected.
Instituto Nacional de Seguridad e Higiene en el Trabajo, Centro Nacional de Condiciones de Trabajo, Dulcet 2-10, E 08034 Barcelona, Spain. atarinma@mtas.es
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Tarín
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Rosell
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Guardino
D000348:Aflatoxins / Q000032:analysis*; D000388:Air / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A