The fundamentals of tissue engineering: new scaffolds.
The ability to regenerate new bone for skeletal use is a major clinical need. In this study, two novel porous calcium phosphate materials pure HA and biphasic HA/beta-Tricalcium phosphate (HA/beta -TCP) were evaluated as potential scaffolds for cell-seeded bone substitutes using human osteoblast-like cells (HOS) and primary human mesenchymal stem cells (hMSCs). A high rate of proliferation was observed on both scaffolds. A greater increase in alkaline phosphatase (ALP- an indicator of osteoblast differentiation) was observed on HA/beta -TCP compared to HA. This observation indicates that HA/TCP may play a role in inducing osteoblastic differentiation. Although further evaluation is required both materials show potential as innovative synthetic substitutes for tissue engineered scaffolds.
Guy's, Kings' and St Thomas' Medical and Dental Institute, King's College London, UK.
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L
Di Silvio
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N
Gurav
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Sambrook
D000818:Animals; D019902:Chondrocytes / Q000166:cytology; D004864:Equipment and Supplies / Q000592:standards; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000592:standards; D007564:Japan; D015603:Keratinocytes / Q000166:cytology; D009924:Organ Culture Techniques / Q000592:standards*; D012449:Safety / Q000592:standards; D023822:Tissue Engineering / Q000592:standards*