Impaired neutralising antibody formation and high transduction efficacy after isolated hepatic perfusion with adenoviral vectors.
Local adenoviral gene transfer can be performed by means of isolated hepatic perfusion (IHP). This methodology is a very effective and safe way to deliver adenoviral vectors. We studied the immune response after IHP. A decreased neutralising antibody formation was observed, offering possibilities for further research in the field of gene therapy in isolated perfusion settings.
Erasmus University Medical Centre-Daniel den Hoed Cancer Centre, Department of Surgical Oncology, PO Box 5201, 3008 AE Rotterdam, The Netherlands.
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B
van Etten
A M M
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Eggermont
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Ambagtsheer
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van Tiel
T L M
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ten Hagen
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D010478:Chemotherapy, Cancer, Regional Perfusion; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008099:Liver / Q000473:pathology / Q000821:virology*; D008297:Male; D009500:Neutralization Tests; D051381:Rats; D011919:Rats, Inbred Strains; D014161:Transduction, Genetic
D000914:Antibodies, Viral