Sensitivity of delta-ALA-D (E.C. 4.2.1.24) of rats to metals in vitro depends on the stage of postnatal growth and tissue.
Heavy metals, like cadmium, lead, and mercury, are potential toxic substances. The exposure to these metals can cause renal disturbances and neurological alterations. Young rats are more sensitive to harmful agents than adult animals. Delta-ALA-D enzyme acts as a biomarker of these exposures, since it has high affinity for divalent metals. The purpose of this search was to investigate the sensitivity of delta-ALA-D from suckling rats to cadmium, lead or mercury in vitro. IC(50) for delta-ALA-D activity of brain, kidneys, and liver from rats with ages between 1 and 6, 8 and 13 or 17 and 21 days was determined using metals concentrations that range from 0 to 200 microM for CdCl(2), 0 to 600 microM for HgCl(2) and from 0 to 50 microM for lead acetate. The results demonstrated that the cerebral delta-ALA-D activity is more sensitive to lead acetate than to cadmium and mercury. Delta-ALA-D from hepatic tissue is the most resistant to presence of mercury chloride in assay medium. Lead and cadmium are more toxic to renal enzyme than mercury. To sum up, the sensitivity of delta-ALA-D enzyme of young rats to heavy metals studied depends on the phase of development and tissue.
Peixoto
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NC
Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria, Campus Universitário-Camobi, 97105-900 Santa Maria, RS, Brazil.
Roza
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T
Pereira
M E
ME
D000367:Age Factors; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008628:Mercury / Q000633:toxicity*; D000623:Porphobilinogen Synthase / Q000032:analysis / Q000187:drug effects* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar / Q000254:growth & development
D015415:Biomarkers; D002104:Cadmium; D007854:Lead; D000623:Porphobilinogen Synthase; D008628:Mercury