Responses of fish chromatophore-based cytosensor to a broad range of biological agents.
A cytosensor based on living chromatophores from Betta splendens Siamese fighting fish was used to test several classes of biologically active agents. Tested agents include neurotransmitters, adenyl cyclase activators, cytoskeleton effectors, cell membrane effectors and protein synthesis inhibitors. Characteristic cell responses were analyzed, and potential cytosensor applications were considered. Streptococcus pyogenes toxins streptolysin S and streptolysin O, Clostridium tetani tetanolysin, Staphylococcus aureus alpha-toxin and Vibrio parahemolyticus hemolysin, all bacterial toxins that act on cell membranes, elicited a strong response from chromatophores. A comparison of purified toxin to actual bacterial culture from Vibrio parahemolyticus demonstrated a nearly identical chromatophore cell response pattern. This suggests that the cytosensor response is reflective of bacterial toxin production.
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA.
Karen P
KP
Dierksen
Ljiljana
L
Mojovic
Bruce A
BA
Caldwell
R Ryan
RR
Preston
Rosalyn
R
Upson
Jeannine
J
Lawrence
Philip N
PN
McFadden
Janine E
JE
Trempy
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D015374:Biosensing Techniques; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000502:physiology; D002856:Chromatophores / Q000502:physiology*; D004784:Environmental Monitoring / Q000379:methods*; D010473:Perciformes / Q000502:physiology; D014118:Toxins, Biological / Q000032:analysis; D014881:Water Supply
D001427:Bacterial Toxins; D014118:Toxins, Biological