Protective effect of rhIL-1beta on pancreatic islets of alloxan-induced diabetic rats.
OBJECTIVE
To observe the protective effect of rhIL-1beta on pancreatic islets of alloxan-induced diabetic rats.


METHODS
Protection of rhIL-1beta on pancreatic islets of alloxan-induced diabetic rats (n = 5) was demonstrated with methods of immunohistochemistry and stereology. The concentration of serum glucose was measured by GOD method and that of serum insulin by RIA.


RESULTS
The concentration of serum glucose increased but that of insulin decreased after administration of alloxan(150 mg/kg), and the volume density and numerical density of the islets were zero. In rhIL-1beta pretreated rats, although the concentration of serum insulin decreased (from 11.9+/-3.0 mIU/L to 6.1+/-1.6 mIU/L, P<0.05), that of glucose was at normal level compared with the control group. As compared with alloxan group, the concentration of serum glucose in rhIL-1beta pretreated rats decreased (from 19.4+/-8.9 mmol/L to 12.0+/-4.0 mmol/L, P<0.05) and the volume density increased(0/L to. 1/L, P<0.05).


CONCLUSIONS
rhIL-1beta pretreatment may have protective effect on the islets of alloxan-induced diabetic rats.
Wu
Li-Ping
LP
Second Department of Geriatrics and Gerontology, Xijing Hospital, Fourth Military Medical University, 169 Changle West Road, Xi'an 710032, Shaanxi Province, China.
Chen
Li-Hua
LH
Zhang
Jin-Shan
JS
Sun
Lan
L
Zhang
Yuan-Qiang
YQ
D000818:Animals; D001786:Blood Glucose; D019610:Cytoprotection; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007375:Interleukin-1 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
D001786:Blood Glucose; D007328:Insulin; D007375:Interleukin-1; D011994:Recombinant Proteins