[Association of homozygous deletion of p15 and p16 gene and amplification of EGFR gene in laryngeal squamous cell carcinoma].
To assess the relationship of deletion of p15 and p16 gene and EGFR gene amplification in laryngeal squamous cell carcinoma (LSCC). DNA was extracted from fresh tumor. Deletion of p15 exon 2(p15E2) and p16 exon 2(p16E2) in 30 cases of LSCC was detected by polymerase chain reaction (PCR) technique. Amplification of EGFR gene in 30 cases of LSCC was detected by FISH. The rate of p15E2 deletion in 30 cases was 13.3(4/30), and that of p16E2 was 16.7% (5/30). p15E2 and p16E2 codeletion rate was 6.7% (2/30). The rate of EGFR gene amplification in 30 cases was 30% (9/30), and was amplified 2 to 8 fold. Homozygous deletion of p16E2 and p15E2 and codeletion is related with amplification of EGFR gene (P = 0.000018), and may play an important role to oncogenesis and malignant progression in LSCC.
Li
Fu-Cai
FC
Department of Medical Genetics, China Medical University, Shenyang 110001, China. Fucaili@hotmail.com
Li
Ying-Hui
YH
Zhao
Xui
X
Fu
Wei-Neng
WN
Xu
Zhen-Ming
ZM
Li
Zeng-Gang
ZG
Sun
Kai-Lai
KL
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D050762:Cyclin-Dependent Kinase Inhibitor p15; D066246:ErbB Receptors / Q000235:genetics*; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D025521:Tumor Suppressor Proteins; D066246:ErbB Receptors