[Differentiation of bone marrow-derived stem cells in injured rat brain tissue].
OBJECTIVE
To investigate the differentiation of bone marrow-derived stem cells (BMDSCs) migrated from blood circulation and resided in the injured brain tissue.


METHODS
Brain injury model was established by iridectomy in the right cerebral cortex of female SD rats. Twenty-four hours after brain injury, the female rats received the implantation of green fluorescence protein (GFP)-labeled BMDSCs from male SD rats and were sacrificed at 2, 4 and 8 weeks after the implantation. Fluorescent immunohistochemistry for CD11b and glial fibrillary acidic protein (GFAP) on the brain sections was used to detect the GFP-positive cells.


RESULTS
One week after the transplantation of the GFP-labeled BMDSCs, 3.53% of the peripheral blood white cells were GFP-positive; at 4 weeks and 8 weeks, a significant number of GFP-positive cells were found at the injury sites, some of which expressed CD11b and others expressed GFAP.


CONCLUSIONS
GFP-labeled BMDSCs can migrate to the injured brain tissue and differentiate into cells that express microglia- and astrocytes-specific antigens.
Zou
Xi-feng
XF
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhang
Hai-yan
HY
Zhao
Chun-li
CL
Li
Tie-lin
TL
Xu
Qun-yuan
QY
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001930:Brain Injuries / Q000473:pathology / Q000601:surgery*; D039481:CD11b Antigen / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D049452:Green Fluorescent Proteins; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009474:Neurons / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins