Influenza viruses resistant to the antiviral drug oseltamivir: transmission studies in ferrets.
Three type A influenza viruses, each of which has a distinct neuraminidase-gene mutation and is resistant to the neuraminidase inhibitor oseltamivir, have been isolated. Previously, in the ferret model, an R292K mutant of a type A (H3N2) virus was not transmitted under conditions in which the wild-type virus was transmitted. This model was used to investigate whether the E119V mutant of a type A (H3N2) virus and the H274Y mutant of a type A (H1N1) virus would be transmitted under similar circumstances. Both mutant viruses were transmitted, although the H274Y mutant required a 100-fold-higher dose for infection of donor ferrets and was transmitted more slowly than was the wild type. Both the mutant and the wild-type viruses retained their genotypic characteristics.
Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA.
M Louise
ML
Herlocher
Rachel
R
Truscon
Stephanie
S
Elias
Hui-Ling
HL
Yen
Noel A
NA
Roberts
Suzanne E
SE
Ohmit
Arnold S
AS
Monto
D000081:Acetamides / Q000494:pharmacology*; D019943:Amino Acid Substitution; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D024882:Drug Resistance, Viral; D005289:Ferrets; D009980:Influenza A virus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000635:transmission* / Q000821:virology*; D053139:Oseltamivir; D014764:Viral Proteins / Q000235:genetics
D000081:Acetamides; D000998:Antiviral Agents; D014764:Viral Proteins; D053139:Oseltamivir; D009439:Neuraminidase