Repressor mutant forms of the Azospirillum brasilense NtrC protein.
The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.
Huergo
Luciano F
LF
Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-990 Curitiba, Paraná, Brazil.
Assumpção
Marcelo C
MC
Souza
Emanuel M
EM
Steffens
M Berenice R
MB
Yates
M Geoffrey
MG
Chubatsu
Leda S
LS
Pedrosa
Fábio O
FO
D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D009586:Nitrogen Fixation / Q000235:genetics; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism; D009604:Nitrosoguanidines / Q000494:pharmacology; D009876:Operon; D051922:PII Nitrogen Regulatory Proteins; D010641:Phenotype; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009566:Nitrates; D009604:Nitrosoguanidines; D051922:PII Nitrogen Regulatory Proteins; D012097:Repressor Proteins; C404143:PIID regulatory protein, Bacteria; D009591:Nitrogenase