Poly(vinyl alcohol) : a potential matrix for glucose oxidase immobilization?
Considerable effort has been focused on the method of immobilizing glucose oxidase (GOD) for amperometric glucose biosensors since the technique employed may influence the available activity of the enzyme and thus affect the performance of the sensor. Narrow measuring range and low current response are still considered problems in this area. In this work, poly(vinyl alcohol)(PVA) was investigated as a potential matrix for GOD immobilization. GOD was entrapped in cross-linked PVA. The use of a PVA-GOD membrane as the enzymatic component of a glucose biosensor was found to be promising in both the magnitude of its signal and its relative stability over time. The optimum PVA-GOD membrane (cross-linking density of 0.06) was obtained through careful selection of the cross-linking density of the PVA matrix.
Department of Bioprocess Engineering, Universiti Teknologi Malaysia, Malaysia.
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Azila
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T
Barbari
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Searson
D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003432:Cross-Linking Reagents; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006801:Humans; D008567:Membranes, Artificial; D011142:Polyvinyl Alcohol; D012680:Sensitivity and Specificity
D001786:Blood Glucose; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011142:Polyvinyl Alcohol; D005949:Glucose Oxidase