[Genetic relationships among five populations of Oxya chinensis in Shanxi Province and adjacent region based on RAPD].
Random Amplified Polymorphic DNA (RAPD) markers were applied to analyze genetic relationships of five populations of Oxya chinensis collected from Shanxi Province and laner Mongolia, Oxya japonica from Guangxi was used as an outgroup. Genomic DNA of sixty-four individuals was extracted from dissected leg muscle using phenol-chloroform procedure, and then amplified by 10 random primers (10 bp), the amplified products were separated by agarose gel electrophoresis. The results were as follows: (1) a total of 115 clear and reproducible bands were generated, molecular size was 200 - 2500 bp. The obtaining segments of individual primer were among 5 - 15, on average, about 12 bands per primer. (2) The dendrogram based on 115 RAPD markers was constructed and clustered using between-groups linkage method. The cluster analysis indicated strong similarities within populations, firstly, the individuals in each population closely clustered together;and then five populations of Oxya chinensis could be distinguished with RAPD markers and were grouped into two distinct clusters. The dendrogram showed that Shanxi Linyi population and Tunliu population were the most similar,which were clustered with Taiyuan population Shanxi into one cluster, while, Daixian population in Shanxi was closely related to laner Mongolia population, both of which belonged to the other cluster. Nevertheless, All the five populations of Oxya chinensis had far genetic distance with Oxya japonica. In the dendrogram, a tendency of clustering following a North-South gradient could be observed, the results implied that genetic distance of five populations of Oxya chinensis correlated with geographical distance to some degree.
Zhang
Jian-Zhen
JZ
College of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
Ren
Li
L
Guo
Ya-Ping
YP
Ma
En-Bo
EB
D000818:Animals; D005828:Genetics, Population; D009987:Orthoptera / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique