[Construction of AFLP molecular markers linkage map and localization of green cocoon gene in silkworm (Bombyx mori)].
Based on an improved method of AFLP, AFLP markers were employed for construction of a linkage map and localization of Gc gene used a set of 44 backcross lines( BC1) of silkworm ( Bombyx mori) as a mapping population. In this work, all together 3 956 bands were obtained by 28 pairs of primers and 141.3 bands each primer pair on average. Among them 2 836 bands were in good agreement with the segregation pattern. A total of 1 018 (25.7%) polymorphic AFLP markers were detected. The 693 (68.1%) of polymorphic markers with 1:1 segregation ratio ( P < or = 0.05) were obtained. Furthermore,The analytical model was based on the backcross type and the parameters were set as following: LOD = 3.0, maximum recombination value of 0. 20 and use the command ' group', 'compare', 'try', 'map' and 'ripple' to construct the linkage maps. 407 of the 693 loci were chi2 tested in agreement with 1:1 segregation were divided into 33 linkages by Mapmaker/Exp(Version 3.0), with a total map distance of 3 676.7 cM and a mean distance of 9.1 cM between markers. The morphological gene Gc was located between L-P4T6-107 and L-PT6T4-84 on linkage group 22. In addition, 286 markers were not included in the linkage groups. The efficiency of loci mapping was 58.7%. Among the 33 linkage groups, the morphological marker Gc classically localized on linkage group 15 was relocated on linkage group 22 on the map, suggesting that this molecular linkage group corresponds to linkage group 15 on the linkage map based on morphological characters. All these have laid an important base for the marker assisted breeding of the silkworm.
The Key Sericultural Laboratory of Agricultural Ministry, Sericulture and Biotechnology Department Southwest Agricultural University, Chongqing 400716, China. zhaoaichun@hotmail.com
Ai-Chun
AC
Zhao
Cheng
C
Lu
Bin
B
Li
Xiao-Ying
XY
Pu
Ze-Yang
ZY
Zhou
Zhong-Huai
ZH
Xiang
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D017344:Genes, Insect; D005819:Genetic Markers; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers