Carbachol-induced membrane ruffling and its desensitization in rat basophilic leukemia (RBL-2H3) cells transfected with m3 muscarinic acetylcholine receptors.
The changes in the reorganization of actin filaments during desensitization of secretion were investigated by transfecting RBL-2H3 cells with cDNA encoding the human m3 muscarinic acetylcholine receptors (RBL-m3 cells). Incubation of RBL-m3 cells with 10-100 microM carbachol in Ca2+ -free medium developed membrane ruffling. When the cells were desensitized under the condition where desensitization of carbachol-induced secretion occurred, desensitized cells failed to develop membrane ruffling with the subsequent addition of carbachol. These results suggest that m3 muscarinic receptor-mediated desensitization of secretion involves negative regulation of actin reorganization leading to membrane ruffling.
Department of Pharmacology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. oishikz@my-pharm.ac.jp
Kazuhiko
K
Oishi
Tomokazu
T
Yoshizumi
Noriyasu
N
Seki
Masaatsu K
MK
Uchida
D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D001491:Basophils / Q000378:metabolism / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007942:Leukemia, Experimental; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D051381:Rats; D043587:Receptor, Muscarinic M3 / Q000819:agonists / Q000187:drug effects* / Q000235:genetics; D015398:Signal Transduction; D014162:Transfection; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000199:Actins; D043587:Receptor, Muscarinic M3; D002217:Carbachol; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium