Improved methods for mutacin detection and production.
Studies of mutacins have always been hampered by the difficulties in obtaining active liquid preparations of these substances. In order to be commercially produced, good mutacin yields have to be obtained, preferably in inexpensive media. The results presented here indicate that mutacins can be produced in supplemented cheese whey permeate. The influence of carbon and nitrogen supplements on mutacin production varied according to the producer strain. The use of CaCO3 as a buffer in batch cultures resulted in improved yields of mutacin in the supernatants. Antimicrobial activity assays were improve by acidification of the diluent (pH 2) and were less variable in peptone water (0.5%). The culture medium consisting of cheese whey permeate (6% w/v), yeast extract (2% w/v) and CaCO3 (1% w/v) was found to be an inexpensive medium for the efficient production of mutacins.
Département de Biochimie et Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec, G1K 7P4, Canada.
Guillaume
G
Nicolas
Isabelle
I
Auger
Martine
M
Beaudoin
Francois
F
Hallé
Hélène
H
Morency
Gisèle
G
LaPointe
Marc C
MC
Lavoie
D001430:Bacteriocins / Q000096:biosynthesis* / Q000378:metabolism*; D002021:Buffers; D002119:Calcium Carbonate; D005690:Galactose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D016982:Micrococcus luteus / Q000254:growth & development; D008894:Milk Proteins / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D013295:Streptococcus mutans / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D000067816:Whey Proteins
D001430:Bacteriocins; D002021:Buffers; D008894:Milk Proteins; D010461:Peptones; D000067816:Whey Proteins; C107503:mutacin B-Ny266; D013395:Sucrose; D002119:Calcium Carbonate; D005690:Galactose