Astrocytic and neuronal biochemical markers in the sera of subjects with diabetes mellitus.
To determine if clinical diabetes is associated with disruption of the blood-brain barrier (BBB) and/or brain injury, enzyme-linked immunoassays and Western blots were used to measure serum levels of S100B, NSE and their auto-antibodies in type 1 and type 2 diabetic subjects. Serum S100B concentrations in type 2 diabetic subjects, but not in type 1 diabetic subjects, were significantly lower than those found in healthy controls. There were no significant differences in serum NSE levels of either type 1 or type 2 diabetics compared to healthy controls. However, there was a significant increase in antibodies to NSE in both type 1 and type 2 diabetic subjects compared to controls, whereas diabetics and controls had equally very low levels of anti S100B auto-antibodies. These studies suggest that diabetes in humans may be associated with alterations in the BBB integrity that allow the emergence of antibodies against neuronal antigens.
Department of Internal Medicine, Saint Louis University Medical School, Saint Louis, MO 63104, USA. mery@mb.sci.am
Meri R
MR
Hovsepyan
Michael J
MJ
Haas
Anna S
AS
Boyajyan
Astghik A
AA
Guevorkyan
Ashot A
AA
Mamikonyan
Susan E
SE
Myers
Arshag D
AD
Mooradian
D000293:Adolescent; D000328:Adult; D000368:Aged; D001253:Astrocytes / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western / Q000379:methods; D002648:Child; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000097:blood*; D009474:Neurons / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*
D015415:Biomarkers; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; D010751:Phosphopyruvate Hydratase