On-line single droplet deposition for MALDI mass spectrometry.
A single droplet generator was coupled with a rotating ball inlet matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometer. Single droplets with 100 picoliter volume were ejected by a piezoelectric-actuated droplet generator and deposited onto a matrix-coated rotating stainless steel ball at atmospheric pressure. The single droplet deposit was transported to the vacuum side of the instrument where ionization was accomplished using a UV pulsed laser. Using this on-line interface, it was possible to obtain protonated molecule signal from as little as 10 fmol analyte.
Zhang
Xia
X
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA.
Narcisse
Damien A
DA
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA.
Murray
Kermit K
KK
Department of Chemistry, Louisiana State University, 232 Choppin Hall, 70803, Baton Rouge, LA, USA. kkmurray@lsu.edu.
D000803:Angiotensin I / Q000737:chemistry; D000818:Animals; D001331:Automation; D001920:Bradykinin / Q000737:chemistry; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D010316:Particle Size; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D007328:Insulin; D045304:Cytochromes c; D000803:Angiotensin I; D001920:Bradykinin