Cephamycin C production is regulated by relA and rsh genes in Streptomyces clavuligerus ATCC27064.
The effects of growth rate and nutrient uptake rate on the production of cephamycin C were determined in the parental strain, deltarelA mutant, and deltarsh null mutant of S. clavuligerus. Production of cephamycin C was inversely related to mycelium growth and the phosphate feed rate was more critical for the production of cephamycin C. On the contrary, the production of cephamycin C was completely abolished in the deltarelA mutant, but not in deltarsh mutant. The changes in the cephamycin C production by disruption of the relA and rsh genes are presumably associated with the consequent ability of the mutants to accumulate (p)ppGpp under nutrient starvation. Therefore, it is concluded that the stringent response of S. clavuligerus to starvation for nutrients is governed mainly by RelA rather than Rsh and that the response is more apparently regulated by the limitation of phosphate.
School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Wook
W
Jin
Hyo Kyung
HK
Kim
Jae Young
JY
Kim
Sung Gyun
SG
Kang
Sang Hee
SH
Lee
Kye Joon
KJ
Lee
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002513:Cephamycins / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005990:Glycerol / Q000378:metabolism*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002513:Cephamycins; D010710:Phosphates; C428123:RshA protein, Streptomyces coelicolor; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases; D005990:Glycerol; C024925:cephamycin C