Regulation of ADF/cofilin phosphorylation and synaptic function by LIM-kinase.
To investigate the role of the LIM-kinase (LIMK) family in the regulation of ADF/cofilin phosphorylation and synaptic function in the mammalian central nervous system (CNS), we conducted biochemical and electrophysiological analysis using mice that were genetically altered in the expression of LIMK-1 and LIMK-2. We showed here that while LIMK-2 knockout mice exhibited minimal abnormalities, the LIMK-1/2 double knockout mice were more severely impaired in both ADF/cofilin phosphorylation and excitatory synaptic function in the CA1 region of the hippocampus. These results indicate a critical role for the LIMK family in the regulation of ADF/cofilin and synaptic function in the brain.
Meng
Yanghong
Y
Brain and Behavior, The Hospital for Sick Children, Room 6028, McMaster Building, Toronto, Ont. M5G 1X8, Canada.
Takahashi
Hisaaki
H
Meng
Jinsong
J
Zhang
Yu
Y
Lu
Guijin
G
Asrar
Suhail
S
Nakamura
Toshikazu
T
Jia
Zhengping
Z
D051339:Actin Depolymerizing Factors; D000818:Animals; D002531:Cerebellum / Q000502:physiology; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051345:Destrin; D006624:Hippocampus / Q000502:physiology; D007700:Kinetics; D054461:Lim Kinases; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*; D016335:Zinc Fingers
D051339:Actin Depolymerizing Factors; D004268:DNA-Binding Proteins; D051345:Destrin; C495825:Dstn protein, mouse; D008840:Microfilament Proteins; D011494:Protein Kinases; D054461:Lim Kinases; C515893:Limk1 protein, mouse; C095325:Limk2 protein, mouse; D017346:Protein Serine-Threonine Kinases