Coupled-enzyme system for the determination of lipase activity.
A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method.
Department of Biological & Chemical Engineering, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia.
Ines Ben
IB
Rejeb
Jeannette Ben
JB
Hamida
Mohamed
M
Gargouri
D002384:Catalysis; D003067:Coenzymes / Q000032:analysis / Q000737:chemistry; D004789:Enzyme Activation; D005227:Fatty Acids / Q000737:chemistry*; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis* / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D014280:Triglycerides / Q000737:chemistry*
D003067:Coenzymes; D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase; D010100:Oxygen; C009564:trilinolein