Pyrosequencing: sequence typing at the speed of light.
Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.
Diggle
Matthew A
MA
Scottish Meningococcus and Pneumococcus Reference Laboratory, Department of Microbiology, House on the Hill, Stobhill Hospital, Balornock Road, Glasgow, G21 3UW.
Clarke
Stuart C
SC
D015373:Bacterial Typing Techniques / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D005798:Genes, Bacterial / Q000235:genetics*; D008954:Models, Biological; D009345:Neisseria meningitidis / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013997:Time Factors
D017931:DNA Primers; D004247:DNA