Determination of neutrophil Fcgamma receptor IIIb antigens (HNA-1a, HNA-1b and HNA-1c) by fluorescence-primed allele-specific polymerase chain reaction.
We evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcgamma receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.
Serviço de Hematologia, do Hospital de Crianças Maria Pia, Porto, Portugal. elisio_costa@hotmail.com
E
E
Costa
M B
MB
Antunes
S
S
Faria
E
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Vieira
R
R
Branca
J
J
Barbot
R
R
Dos Santos
D000483:Alleles; D000941:Antigens / Q000032:analysis*; D015703:Antigens, CD / Q000276:immunology*; D005453:Fluorescence; D058851:GPI-Linked Proteins; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011174:Portugal / Q000453:epidemiology; D017452:Receptors, IgG / Q000276:immunology*
D000941:Antigens; D015703:Antigens, CD; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG