Activation of phenylpropanoid metabolism in sesame by over-expression of carrot calmodulin gene.
Transgenic sesame (Sesamum schinzianum ASCH.) was produced by Agrobacterium-mediated transfection of a carrot calmodulin gene, cam-4, which was specifically expressed upon the contact of carrot cells with oligogalacturonide elicitor. Coding region of cam-4 was ligated to the downstream of 35S promoter of cauliflower mosaic virus and subcloned into pMATGBO-DB3.1. A. tumefaciens 4404 was transformed with the constructed vector, and the crown gall tissues formed in the sesame seedlings were transferred onto appropriate media to obtain the re-differentiated plants. The reverse-transcription polymerase chain reaction followed by Southern blot analysis revealed that cam-4 gene was appreciably expressed in the transgenic plants. Activities of two key enzyme regulating phenylpropanoid metabolisms, phenylalanine ammonia-lyase and caffeic acid O-methyltransferase, and the contents of phenolic compounds in the transformed sesame were markedly elevated as compared with those of the control. These results suggest that the over-expression of cam-4 gene enhances the biosynthetic activities of phenylpropane derivatives in the transformed sesame plants.
Mitsuma
Satoru
S
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Ishigaki
Eriko
E
Sugiyama
Ryuji
R
Asamizu
Tetsuya
T
Yamada
Kyoji
K
Kurosaki
Fumiya
F
D016960:Agrobacterium tumefaciens / Q000235:genetics; D015139:Blotting, Southern; D002147:Calmodulin / Q000235:genetics*; D018552:Daucus carota / Q000235:genetics*; D008780:Methyltransferases / Q000096:biosynthesis; D009569:Nitric Oxide / Q000096:biosynthesis; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis; D010666:Phenylpropionates / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds; D031685:Sesamum / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
D002147:Calmodulin; D010666:Phenylpropionates; D009569:Nitric Oxide; D008780:Methyltransferases; C019608:caffeate O-methyltransferase; D010650:Phenylalanine Ammonia-Lyase