Expansion of CCR5+ CD4+ T-lymphocytes in the course of active pulmonary tuberculosis.
Nonsyncytium inducing, macrophage tropic HIV strains predominate in the course of active tuberculosis (TB). The present study assesses the expression of CCR5 in CD4+ T-lymphocytes from blood and bronchoalveolar lavage (BAL) of TB patients, non-TB lung disease controls and healthy controls. Memory (CD45RO+), recently activated (CD69+), proliferating (Ki67+) CCR5+ or CCR3+ CD4+ T-lymphocytes were determined by multiparametric flow cytometry analysis. Results show that BAL CD4+ T-lymphocytes expressing CCR5 or CCR3 were significantly increased when compared to peripheral blood both in patients and in healthy controls. However, the data show that the proportions of peripheral blood CCR5+ CD4+ and CCR3+ CD4+ T-lymphocytes and BAL CCR5+ CD4+ T-lymphocytes, but not BAL CCR3+ CD4+ T-lymphocytes, were significantly increased in TB patients. Furthermore, the observation that BAL CCR5+ CD4+ T-lymphocytes from TB patients expressed early activation markers, were not proliferating and showed down-regulation of CCR5 expression suggests recruitment and trapping at the site of disease. Altogether, these results suggest that the lower respiratory tract mucosa may provide cellular targets accessible for efficient transmission of macrophage tropic HIV-1 variants and that tuberculosis may enhance this phenomenon.
Santucci
M B
MB
Dept of Biology, University of Roma "Tor Vergata", Via della Ricerca Scientifica 00133, Roma, Italy. fraziano@bio.uniroma2.it
Bocchino
M
M
Garg
S K
SK
Marruchella
A
A
Colizzi
V
V
Saltini
C
C
Fraziano
M
M
D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054397:Receptors, CCR3; D019713:Receptors, CCR5 / Q000276:immunology*; D019707:Receptors, Chemokine / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
C515350:CCR3 protein, human; D054397:Receptors, CCR3; D019713:Receptors, CCR5; D019707:Receptors, Chemokine