Homologies among three Bacillus licheniformis plasmids and molecular characterization of their replication module.
To assess to what extent three Bacillus licheniformis plasmids had the same molecular organization a physical map of the 9.34, 8.40 and 7.90 kb plasmids was achieved by using seventeen restriction enzymes. Southern hybridization was performed on plasmids using restriction fragments of the smallest plasmid as probes. Data from different hybridization patterns show a close homology among the three plasmids hypothesizing a similar molecular organization. The lack of plasmid diversity observed, seem to support the hypothesis of a similar phylogeny among these plasmids. This investigation provides more information concerning phylogeny, interrelationships and level of diversity among Bacillus plasmids and a molecular characterization of three plasmids useful for the construction of cloning vectors.
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milan, Via Celoria 2, Milan 20133, Italy. carlo.parini@unimi.it
Carlo
C
Parini
Simone
S
Guglielmetti
Diego
D
Mora
Giovanni
G
Ricci
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D012093:Replicon / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
D004269:DNA, Bacterial