Kinetics of expression of the salivary apyrases in Triatoma infestans.
Apyrases are nucleoside triphosphate-diphosphohydrolases that remove Pi from ATP and ADP. The blood feeding reduviid Triatoma infestans, which transmits the Trypanosoma cruzi agent of Chagas disease to animals and man, presents in its salivary glands five apyrases with molecular masses of 88, 82, 79, 68 and 67 kDa. These triatomine apyrases have been associated with the prevention of ADP induced platelet aggregation in the host. Here we provide biochemical data showing that these apyrases are stored in the lumen of the salivary gland D1 pairs, and that about one half of the pool of the enzyme is consumed during feeding. After the feeding recovery of apyrases to maximal activity level takes days, thus suggesting de novo protein synthesis. This hypothesis is supported by quantitative RT-PCR analysis which shows an upregulation of the 79 kDa apyrase mRNA level after feeding.
Faudry
Eric
E
Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, 70910-900 Brasilia, Brazil.
Rocha
Priscila S
PS
Vernet
Thierry
T
Lozzi
Silene P
SP
Teixeira
Antonio R L
AR
D000818:Animals; D001081:Apyrase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001769:Blood; D014355:Chagas Disease / Q000635:transmission; D004247:DNA / Q000235:genetics; D004435:Eating; D015870:Gene Expression; D006801:Humans; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics / Q000469:parasitology; D007700:Kinetics; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000201:enzymology; D014224:Triatoma / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000472:pathogenicity
D012333:RNA, Messenger; D004247:DNA; D001081:Apyrase