Molecular characterization of two lactate dehydrogenase genes with a novel structural organization on the genome of Lactobacillus sp. strain MONT4.
Two lactate dehydrogenase (ldh) genes from Lactobacillus sp. strain MONT4 were cloned by complementation in Escherichia coli DC1368 (ldh pfl) and were sequenced. The sequence analysis revealed a novel genomic organization of the ldh genes. Subcloning of the individual ldh genes and their Northern blot analyses indicated that the genes are monocistronic.
Department of Food Science and Toxicology, University of Idaho, Moscow, ID 83844-2312, USA.
Jennifer
J
Weekes
Gülhan U
GU
Yüksel
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000302:isolation & purification; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D027843:Vitis / Q000382:microbiology
D011994:Recombinant Proteins; D007770:L-Lactate Dehydrogenase