Characterization of hemizygous SOD1/wild-type transgenic mice with the SHIRPA primary screen and tests of sensorimotor function and anxiety.
SOD1 is one of several overexpressed genes in Down's syndrome. In order to dissect genetic causes of the syndrome, hemizygous human wild-type SOD1 transgenic mice were compared to FVB/N non-transgenic controls at 3 months of age in the SHIRPA primary screen of neurologic function as well as in tests of motor activity and coordination. The responsiveness of SOD1/wt transgenic mice to visual and somatosensory stimuli was reduced in placing, pinna, corneal, and toe-pinch tests. In addition, SOD1/wt transgenic mice crossed fewer segments on a stationary beam. On the contrary, there was no intergroup difference for motor activity and anxiety in open-field and emergence tests and for latencies before falling on the stationary beam, coat-hanger, and rotorod. These results indicate mild deficits in sensorimotor responsiveness in a mouse model expressing human SOD1 and that the overexpressed gene may be responsible for some Down symptoms.
Faculté de Médecine et de Pharmacie, Université de Rouen, INSERM U614, Bâtiment de Recherche, 22 bld Gambetta, Salle 1D18, 76183 Rouen, Cedex, France. robert.lalonde@univ-rouen.fr
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Lalonde
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Dumont
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Paly
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London
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Strazielle
D000818:Animals; D001008:Anxiety Disorders / Q000235:genetics* / Q000503:physiopathology; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000235:genetics* / Q000503:physiopathology / Q000523:psychology*; D005106:Exploratory Behavior / Q000502:physiology; D005260:Female; D006801:Humans; D007175:Impulsive Behavior / Q000235:genetics / Q000503:physiopathology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity / Q000235:genetics; D009069:Movement Disorders / Q000175:diagnosis / Q000235:genetics; D009460:Neurologic Examination / Q000379:methods; D010147:Pain Measurement / Q000379:methods; D010812:Physical Stimulation; D020886:Somatosensory Disorders / Q000235:genetics* / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1
C000606290:SOD1 protein, human; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1