Photo-immobilization of a phospholipid polymer for surface modification.
A photo-reactive polymer having a phospholipid polar group was prepared, and the polymer was photo-immobilized on polymeric surfaces, where its interactions with biocomponents were investigated. By using a photo-immobilization method, the polymer was used for surface modification of polyethylene and polypropylene, polymers whose surfaces were not treated in our previous development of the phosphorylcholine-derived polymer. The photo-reactive polymer was synthesized by a coupling reaction involving copolymer consisting of 2-methacryloyloxyethyl phosphorylcholine and methacrylic acid with 4-azidoaniline. When the polymer was unpattern immobilized on the surface, X-ray photo-electron spectroscopic analysis and static contact angle measurements were performed. It was shown that the surface was covered with phospholipid polar groups. Micropattern immobilization was carried out using a micropatterned photo-mask. Measurements using atomic force microscopy showed that the swelled micropatterned polymer was five times as thick as the dried one. Protein adsorption and platelet adhesion were reduced on the polymer-immobilized regions. Mammalian cells did not adhere, and formed aggregates on the immobilized regions. In conclusion, the photo-reactive phospholipid polymer was covalently immobilized on the conventional polymer surfaces and it tended to reduce interactions with proteins and cells.
Konno
Tomohiro
T
Regenerative Medical Bioreactor Project, Kanagawa Academy of Science and Technology, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.
Hasuda
Hirokazu
H
Ishihara
Kazuhiko
K
Ito
Yoshihiro
Y
D000327:Adsorption / Q000528:radiation effects; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry; D008027:Light; D008422:Materials Testing; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000502:physiology; D010743:Phospholipids / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry / Q000379:methods*; D010973:Platelet Adhesiveness / Q000502:physiology*; D020959:Polyethylene / Q000737:chemistry* / Q000528:radiation effects; D011126:Polypropylenes / Q000737:chemistry* / Q000528:radiation effects; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D020099:Coated Materials, Biocompatible; D007136:Immunoglobulins; D010743:Phospholipids; D011126:Polypropylenes; D012710:Serum Albumin, Bovine; D020959:Polyethylene