Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures.
Studies on CFTR protein expression and localization in native tissues or in primary cultures of human epithelial cells are scarce due to the intrinsic instability of this protein, its low expression in most tissues and also to technical difficulties. However, such data are of the highest importance to understand the pathophysiology of CF. The purpose of this article is to outline several assays for the characterization of primary epithelial cultures and to review different CFTR immunostaining protocols.
Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge, Av Padre Cruz, 1649-016, Lisboa, Portugal. filipa.mendes@insa.min-saude.pt
Filipa
F
Mendes
Laurent
L
Doucet
Alexandre
A
Hinzpeter
Claude
C
Férec
Joanna
J
Lipecka
Janine
J
Fritsch
Aleksander
A
Edelman
Huub
H
Jorna
Rob
R
Willemsen
Alice G M
AG
Bot
Hugo R
HR
De Jonge
Jocelyne
J
Hinnrasky
Nicolas
N
Castillon
Karima
K
Taouil
Edith
E
Puchelle
Deborah
D
Penque
Margarida D
MD
Amaral
D018929:Cell Culture Techniques; D003550:Cystic Fibrosis / Q000235:genetics / Q000276:immunology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000276:immunology*; D004847:Epithelial Cells; D015870:Gene Expression / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator