Attenuation of cocaine-induced conditioned place preference by Polygala tenuifolia root extract.
A recent investigation indicated that Polygala tenuifolia Willdenow extract (PTE) possesses a potential antipsychotic effect. In this study, we examined the effects of PTE on the cocaine-induced changes in locomotor activity, conditioned place preference (CPP), fos-related antigen-immunoreactivity (FRA-IR), and activator protein (AP)-1 DNA binding activity. Cocaine-induced behavioral effects (hyperlocomotion and CPP) occurred in parallel with increases in FRA-IR and AP-1 DNA binding activity in the nucleus accumbens. These responses induced by cocaine were consistently attenuated by concurrent treatment with PTE (25 mg or 50 mg/kg/day, i.p. x 7). The adenosine A2A receptor antagonist, 1,3,7-trimethyl-8-(3-chlorostyrl)xanthine (0.5 or 1.0 mg/kg, i.p.), reversed the PTE-mediated pharmacological action in a dose related manner; neither the adenosine A(1) receptor antagonist, 8-cyclopentyl-1,3-dimethylxanthine (0.5 or 1.0 mg/kg, i.p.) nor the A2B receptor antagonist, alloxazine (1.5 or 3.0 mg/kg, i.p.) significantly affected this pharmacological action. Our results suggest that PTE prevents cocaine-induced behavioral effects, at least in part, via the activation of the adenosine A2A receptor.
Neurotoxicology Program, College of Pharmacy, Korea Institute of Drug Abuse, Kangwon National University, Chunchon 200-701, South Korea.
Eun-Joo
EJ
Shin
Ki-Wan
KW
Oh
Kee-Won
KW
Kim
Yong Soo
YS
Kwon
Jin Hyeong
JH
Jhoo
Wang-Kee
WK
Jhoo
Joo-Young
JY
Cha
Yong Kwang
YK
Lim
In Soon
IS
Kim
Hyoung-Chun
HC
Kim
D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography; D003042:Cocaine / Q000037:antagonists & inhibitors*; D004247:DNA / Q000378:metabolism; D007150:Immunohistochemistry; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009714:Nucleus Accumbens / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D031801:Polygala / Q000737:chemistry*; D016760:Proto-Oncogene Proteins c-fos / Q000276:immunology; D058915:Purinergic P1 Receptor Antagonists; D013037:Spatial Behavior / Q000187:drug effects*; D018808:Transcription Factor AP-1 / Q000378:metabolism
D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D058915:Purinergic P1 Receptor Antagonists; D018808:Transcription Factor AP-1; D004247:DNA; D003042:Cocaine