Involvement of CD14 in lipopolysaccharide- induced liver injury in mice pretreated with Propionibacterium acnes.
OBJECTIVE
The aim of this study was to elucidate the role of CD14 in the Propionibacterium acnes-lipopolysaccharide (LPS) system.


RESULTS
CD14 transgenic mice (M14M), which expressed heterotopic CD14 and showed decreased responses to LPS in vivo, were used. Seven days after priming, the size of granulomas induced by an intraperitoneal administration of P. acnes in the M14M mice was smaller than that in the nontransgenic mice. The number of CD14-positive cells in granulomas was also decreased in the M14M mice compared to the nontransgenic mice. An LPS challenge induced apoptotic and necrotic changes in hepatocytes in the nontransgenic mice but not in the M14M mice. Seven days after priming, tumor necrosis factor-alpha expression was found in monocytic cells in granulomas and Kupffer cells in the nontransgenic mice and was significantly upregulated after LPS injection, whereas the expression was very weak in these cells in the M14M mice.


CONCLUSIONS
CD14 plays a role in the P. acnes-LPS system in both priming and induction phases.
Department of Immunology and Allergy, Oita University Medical School, Oita, Japan.
Tomohisa
T
Uchida
Masashi
M
Kataoka
Yasunori
Y
Higuchi
Keiko
K
Matsuura
Naoki
N
Hijiya
Shunsuke
S
Yamamoto
D000818:Animals; D017209:Apoptosis; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D006099:Granuloma / Q000209:etiology; D022781:Hepatocytes / Q000473:pathology; D007274:Injections, Intraperitoneal; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009000:Monocytes / Q000378:metabolism; D009336:Necrosis; D011425:Propionibacterium acnes / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D018076:DNA, Complementary; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha