Fishery by-product as a nutrient source for bacteria and archaea growth media.
A highly soluble fish protein hydrolysates (FPH) with an 80% protein (peptide size between 1.5 and 20 kDa) and a low free amino acid content was obtained from hake (Merluccius hubssi) filleting waste [Lat. Am. Appl. Res. 30 (2000) 241]. Assays with Halobacterium salinarum, Escherichia coli, Bacillus subtilis and Staphylococcus epidermidis were performed in order to test that FPH as nutrient source for archaea and eubacteria culture media. Cell growth was evaluated by plate count, and by monitoring turbidity and nucleic acids content in liquid cultures. Neither cell growth nor generation times resulting from control and FPH cultures exhibited statistically significant differences at alpha: 0.05 suggesting that FPH can be used as an alternative substrate for microorganism cultural purposes.
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Funes 3250, 4 nivel, B 7602 AYJ Mar del Plata, Argentina. cmartone@mdp.edu.ar
Celina B
CB
Martone
Olinda
O
Pérez Borla
Jorge J
JJ
Sánchez
D000704:Analysis of Variance; D000818:Animals; D001105:Archaea / Q000254:growth & development*; D001419:Bacteria / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000378:metabolism*; D005398:Fisheries; D048388:Gadiformes / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D009696:Nucleic Acids / Q000378:metabolism; D011492:Protein Hydrolysates / Q000302:isolation & purification / Q000378:metabolism*; D014866:Waste Products
D003470:Culture Media; D009696:Nucleic Acids; D011492:Protein Hydrolysates; D014866:Waste Products