Inhibition of herpesvirus-6B RNA replication by short interference RNAs.
RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.
Yoon
Jong-Sub
JS
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
Kim
Sun-Hwa
SH
Shin
Min-Chu
MC
Hong
Seong-Karp
SK
Jung
Yong-Tae
YT
Khang
In-Gu
IG
Shin
Wan-Shik
WS
Kim
Chun-Choo
CC
Paik
Soon-Young
SY
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D012330:RNA, Double-Stranded / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology; D014779:Virus Replication
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D012367:RNA, Viral