Superoxide production from human polymorphonuclear leukocytes by human mannan-binding protein (MBP).
Mannan-binding protein (MBP) is a Ca(2+)-dependent mammalian lectin that plays an important role in innate immunity. In this study, we found that ligand-bound MBP stimulates polymorphonuclear leukocytes (PMN) to induce cell aggregation and superoxide production. The biological response of PMN to ligand-bound MBP was dose- and time-dependent. The PMN aggregation and superoxide production induced by ligand-bound MBP was blocked completely by pertussis toxin, and partially blocked by a platelet activation factor receptor antagonist, TCV-309. These findings suggest that the ligand-bound MBP stimulates PMN through a putative MBP receptor(s) on PMN.
Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501 Japan.
Kazuhide
K
Uemura
Harumi
H
Yamamoto
Tomoaki
T
Nakagawa
Kishiko
K
Nakamura
Nobuko
N
Kawasaki
Shogo
S
Oka
Bruce Yong
BY
Ma
Toshisuke
T
Kawasaki
D002118:Calcium / Q000378:metabolism; D002449:Cell Aggregation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008024:Ligands; D008358:Mannose / Q000737:chemistry; D037601:Mannose-Binding Lectin / Q000096:biosynthesis*; D009504:Neutrophils / Q000378:metabolism*; D037342:Pertussis Toxin / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000378:metabolism; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011145:Polyvinyls / Q000737:chemistry; D011726:Pyridinium Compounds / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism*; D044005:Tetrahydroisoquinolines / Q000494:pharmacology; D013997:Time Factors
D008024:Ligands; D037601:Mannose-Binding Lectin; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011145:Polyvinyls; D011726:Pyridinium Compounds; D011994:Recombinant Proteins; D044005:Tetrahydroisoquinolines; D013481:Superoxides; D037342:Pertussis Toxin; D008358:Mannose; C067430:TCV 309; D002118:Calcium