Pyrimidine 5'-nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography.
A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'-nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'-azido-3'-deoxythymidine as the phosphate acceptor.
Dipartimento di Biochimica "A. Castellani", Università di Pavia, Italy.
Chiara
C
Zanone
Laurent R
LR
Chiarelli
Giovanna
G
Valentini
Eleonora
E
Perani
Laura
L
Annovazzi
Simona
S
Viglio
Paolo
P
Iadarola
D015720:5'-Nucleotidase / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology; D013058:Mass Spectrometry; D011994:Recombinant Proteins / Q000097:blood; D015215:Zidovudine / Q000494:pharmacology
D011994:Recombinant Proteins; D015215:Zidovudine; D015720:5'-Nucleotidase