Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study.
Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.
Norazril
S A H
SA
Tissue Engineering Laboratory, Hospital Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Norhayati
M M
MM
Mazlyzam
A L
AL
Fauziah
O
O
Ruszymah
B H I
BH
D002448:Cell Adhesion / Q000502:physiology*; D048271:Chitosan; D003094:Collagen; D004734:Energy Metabolism / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques / Q000379:methods*; D011108:Polymers; D023822:Tissue Engineering / Q000379:methods*
D011108:Polymers; D003094:Collagen; D048271:Chitosan