An isocoumarin with hepatoprotective activity in Hep G2 and primary hepatocytes from Agrimonia pilosa.
Phytochemical investigation of the aqueous extract of the roots of Agrimonia pilosa Ledeb. (Rosaceae), as guided by hepatoprotective activity in vitro, furnished two isocoumarins, agrimonolide (1) and agrimonolide 6-O-beta-D-glucoside (3), and (+)-catechin (2). Compound 1 showed hepatoprotective effects on both tacrine-induced cytotoxicity in human liver-derived Hep G2 cells and tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes with EC50 values of 88.2 +/- 2.8 and 37.7 +/- 1.6 microM, respectively.
College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
Eun-Jeon
EJ
Park
Hyuncheol
H
Oh
Tai-Hyun
TH
Kang
Dong-Hwan
DH
Sohn
Youn-Chul
YC
Kim
D031981:Agrimonia; D000818:Animals; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013619:Tacrine / Q000633:toxicity; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D003374:Coumarins; D010936:Plant Extracts; D013619:Tacrine; D020122:tert-Butylhydroperoxide