In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte.
In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER; among them, three are localized in a 300 kb cluster.
Physiologie de la Reproduction et des Comportements, UMR 6175 INRA-CNRS-Université François Rabelais de Tours-Haras Nationaux, 37380 Nouzilly, France.
Sébastien
S
Dadé
Isabelle
I
Callebaut
Amélie
A
Paillisson
Martine
M
Bontoux
Rozenn
R
Dalbiès-Tran
Philippe
P
Monget
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism*; D020224:Expressed Sequence Tags; D015723:Gene Library; D017403:In Situ Hybridization; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D009865:Oocytes / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000941:Antigens; D018076:DNA, Complementary; D004527:Egg Proteins; C416503:Nalp5 protein, mouse; D012333:RNA, Messenger