An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer.
Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca(2+)/Mg(2+)-free fusion medium and a serum-free medium being used for bovine embryos.
Chang
Kyung H
KH
School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Lim
Jeong M
JM
Kang
Sung K
SK
Lee
Byeong C
BC
Moon
Shin Y
SY
Hwang
Woo S
WS
D000818:Animals; D002417:Cattle / Q000196:embryology*; D018929:Cell Culture Techniques; D002459:Cell Fusion / Q000379:methods*; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology; D009865:Oocytes / Q000166:cytology*; D011446:Prospective Studies; D011897:Random Allocation; D014470:Umbilical Cord / Q000166:cytology
D003470:Culture Media