Antioxidant activity of jionoside D from Clerodendron trichotomum.
The antioxidant property of jionoside D, isolated from Clerodendron trichotomum (Verbenaceae), was investigated. This compound showed scavenging activity of intracellular reactive oxygen species and of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, as well as lipid peroxidation inhibitory activity. This radical scavenging activity of jionoside D protected the cell viability of Chinese hamster lung fibroblast (V79-4) cells exposed H2O2. Furthermore, jionoside D reduced the apoptotic cells induced by H2O2, as demonstrated by the decreased number of sub G1 hypo-diploid cells and apoptotic body formation. However, it increased the activities of cellular antioxidant enzymes, superoxide dismutase and catalase. Taken together, these findings suggest that jionoside D, isolated from C. trichotomum, exhibits antioxidant properties.
Chae
Sungwook
S
Natural Products Research Institute, Seoul National University, Yeunkun dong, Chongno ku, Korea.
Kim
Ju Sun
JS
Kang
Kyoung Ah
KA
Bu
Hyung Deuk
HD
Lee
Youngki
Y
Hyun
Jin Won
JW
Kang
Sam Sik
SS
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D032409:Clerodendrum; D006224:Cricetinae; D003412:Cricetulus; D004187:Disaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006834:Hydrazines / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D004187:Disaccharides; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C501789:jionoside D; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase