Patterning multiple aligned self-assembled monolayers using light.
This work describes a method for patterning a gold substrate with multiple, aligned self-assembled monolayers (SAMs) using light at different wavelengths. It describes the synthesis and characterization of an alkanethiolate SAM that is photosensitive to light at both 220 and 365 nm. A photomask acts as an area-selective filter for light at 220 and 365 nm, and a single set of exposures at these two wavelengths through one photomask, without steps of alignment between the exposures, can produce three aligned SAMs on one gold substrate. We demonstrate the versatility of this method of photopatterning by modifying individual aligned SAMs chemically to produce surfaces having different properties. We characterize the modified SAMs using immunolabeling, matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy, and surface plasmon resonance spectroscopy. We also describe the patterning of two aligned SAMs that resist the adsorption of proteins and a third region that does not resist the adsorption of proteins. The ability to produce multiple, aligned patterns of SAMs in a single step, without alignment of photomasks in separate steps, increases the versatility of SAMs for studying a range of physical phenomena.
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Declan
D
Ryan
Babak Amir
BA
Parviz
Vincent
V
Linder
Vincent
V
Semetey
Samuel K
SK
Sia
Jing
J
Su
Milan
M
Mrksich
George M
GM
Whitesides
D000327:Adsorption; D006046:Gold / Q000737:chemistry / Q000528:radiation effects; D008567:Membranes, Artificial; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects*; D013499:Surface Properties; D014466:Ultraviolet Rays
D008567:Membranes, Artificial; D011108:Polymers; D013438:Sulfhydryl Compounds; D006046:Gold