[Obtainment of transgenic wheat with the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) gene and analysis of resistance to aphid].
Snowdrop lectin (Galanthus nivalis agglutinin; GNA) is toxic to sap sucking injurious insects of Homopteran. A new gna gene has been transferred into common spring wheat Zhong60634 and winter wheat Yumai66 with high yield by using the biolistic transformation method. Transgenic wheat plants have been obtained in both of the two varieties. Two transgenic plants (T0) have been obtained from the bombarded 535 immature embryos of Zhong60634. Bioassay results show that the development of aphid could be slowed down and the survival rate of young aphid could be reduced by gna gene. Seventeen transgenic plants (T0) were obtained from the bombarded 4636 immature embryos of Yumai66. Twenty plantlets with good resistance to Rhopalosiphum padi and Macrosiphum avenae, which are mainly aphid in north wheat area, were identified from the transgenic plants of T1 generation that came from 8 T0 transgenic plants with good resistance to aphid. The anti-aphid bioassay shows that resistance to the different grain aphid is not the same in transgenic wheat plants. To Rhopalosiphum padi, the rate of survival aphid 8 days after exposing transgenic plants to aphids is significantly lower than that of nontransgenic plants. To Macrosiphum avenae, growth speed of aphids is slowed down but not killed. At the same time, the death rate of young aphids is increased. Anyway, feeding of the two kinds of aphids has been controlled in a certain degree by gna gene when aphids can free to move in plants.
Liang
Hui
H
College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Zhu
Yin-Feng
YF
Zhu
Zhen
Z
Sun
Dong-Fa
DF
Jia
Xu
X
D000818:Animals; D001042:Aphids / Q000254:growth & development*; D007306:Insecticides; D037241:Mannose-Binding Lectins / Q000235:genetics*; D010572:Pest Control, Biological; D037121:Plant Lectins / Q000235:genetics*; D030821:Plants, Genetically Modified; D014908:Triticum / Q000235:genetics* / Q000469:parasitology
D007306:Insecticides; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C063117:snowdrop lectin