Control of dendritic branching and tiling by the Tricornered-kinase/Furry signaling pathway in Drosophila sensory neurons.
To cover the receptive field completely but without redundancy, neurons of certain functional groups exhibit tiling of their dendrites via dendritic repulsion. Here we show that two evolutionarily conserved proteins, the Tricornered (Trc) kinase and Furry (Fry), are essential for tiling and branching control of Drosophila sensory neuron dendrites. Dendrites of fry and trc mutants display excessive terminal branching and fail to avoid homologous dendritic branches, resulting in significant overlap of the dendritic fields. Trc control of dendritic branching involves regulation of RacGTPase, a pathway distinct from the action of Trc in tiling. Timelapse analysis further reveals a specific loss of the ability of growing dendrites to turn away from nearby dendritic branches in fry mutants, suggestive of a defect in like-repels-like avoidance. Thus, the Trc/Fry signaling pathway plays a key role in patterning dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching.
Emoto
Kazuo
K
Howard Hughes Medical Institute, Department of Physiology and Biochemistry, University of California San Francisco, 1550 4th Street, San Francisco, CA 94143, USA.
He
Ying
Y
Ye
Bing
B
Grueber
Wesley B
WB
Adler
Paul N
PN
Jan
Lily Yeh
LY
Jan
Yuh-Nung
YN
D000595:Amino Acid Sequence; D000818:Animals; D048430:Cell Shape; D003712:Dendrites / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000145:classification / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology*; D020744:rac GTP-Binding Proteins / Q000378:metabolism
D029721:Drosophila Proteins; C436143:fry protein, Drosophila; C419769:trc protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D020744:rac GTP-Binding Proteins