The nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals.
Plants use the family of phytochrome photoreceptors to sense their light environment in the red/far-red region of the spectrum. Phytochrome A (phyA) is the primary photoreceptor that regulates germination and early seedling development. This phytochrome mediates seedling de-etiolation for the developmental transition from heterotrophic to photoauxotrophic growth. High intensity far-red light provides a way to specifically assess the role of phyA in this process and was used to isolate phyA-signaling intermediates. fhy1 and pat3 (renamed fhy1-3) are independently isolated alleles of a gene encoding a phyA signal transduction component. FHY1 is a small 24 kDa protein that shows no homology to known functional motifs, besides a small conserved septin-related domain at the C-terminus, a putative nuclear localization signal (NLS) and a putative nuclear exclusion signal (NES). Here we demonstrate that the septin-related domain is important for FHY1 to transmit phyA signals. Moreover, the putative NLS and NES of FHY1 are indeed involved in its nuclear localization and exclusion. Nuclear localization of FHY1 is needed for it to execute responses downstream of phyA. Together with the results from global expression analysis, our findings point to an important role of FHY1 in phyA signaling through its nuclear translocation and induction of gene expression.
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Mathias
M
Zeidler
Qingwen
Q
Zhou
Xavier
X
Sarda
Chi-Ping
CP
Yau
Nam-Hai
NH
Chua
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D004307:Dose-Response Relationship, Radiation; D018506:Gene Expression Regulation, Plant; D008027:Light; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D010834:Phytochrome / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D051942:Phytochrome A; D030821:Plants, Genetically Modified; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013045:Species Specificity
D029681:Arabidopsis Proteins; C441269:FHY1 protein, Arabidopsis; D019913:Nuclear Localization Signals; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D010834:Phytochrome