Multiplexed hybridization detection of quantum dot-conjugated DNA sequences using surface plasmon enhanced fluorescence microscopy and spectrometry.
In this study, the general suitability of quantum dot (QD)-DNA conjugates for the surface plasmon enhanced fluorescence spectroscopy technique is demonstrated. Furthermore, the QD-DNA system is transferred to the platform of surface plasmon enhanced fluorescence microscopy. Using this technique together with a microarray format, in which the sensor-bound single-stranded catcher probes are organized in individual surface spots, results in a simultaneous qualitative analysis of QD-conjugated analyte DNA strands as multicolor images. A clear decomposition of different QD(x)()-DNA(y)() mixtures can be achieved for sequential, as well as mixture injections. Besides this, the study describes the successful approach of measuring spectrally resolved surface plasmon enhanced fluorescence signals derived from catcher probe hybridized QD-DNA conjugates.
Department of Chemistry and of Materials Science, National University of Singapore, Kent Ridge Road, Singapore. rudolf-robelek@imre.a-star.edu.sg
Rudolf
R
Robelek
Lifang
L
Niu
Evelyne L
EL
Schmid
Wolfgang
W
Knoll
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009693:Nucleic Acid Hybridization; D011789:Quantum Theory; D013057:Spectrum Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
D004277:DNA, Single-Stranded