4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH.
Thymocytes exposed to the pro-oxidant tert-butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane-bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.
Kossowka
Ewa
E
Department of Biochemistry, Medical University, Gdańsk, Poland.
Zauszkiewicz
Agata
A
Kubasik-Juraniec
Jolanta
J
Tukaj
Cecylia
C
Spodnik
Jan Henry
JH
Hallman
Anna
A
Klimek
Jerzy
J
Syta
Emilia
E
Figarski
Adam
A
Wakabayashi
Takashi
T
Woźniak
Michał
M
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D048430:Cell Shape; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000378:metabolism*
D000975:Antioxidants; D003497:Cyclic N-Oxides; D020122:tert-Butylhydroperoxide; C003959:TEMPO