Immunogenicity of murein-associated proteins from temperature-stressed Streptococcus suis cultures.
We compared immunogenicity in pigs of whole cell lysate proteins (WCP) with murein-associated proteins (MAP) obtained from a virulent serotype 2 strain of Streptococcus (S.) suis grown at 32 or 42 degrees C. Protein fractions were tested for their ability to induce antibodies in 3-week-old piglets by enzyme-linked immunosorbent assay and Western blot analysis. We found a significant increase in the antibody levels in all sera irrespective of the preparation used for immunization. However, alpha-WCP sera showed higher reactivities than alpha-MAP sera, and piglets immunized with 32 degrees C preparations (alpha-32 sera) showed higher responses than those immunized with 42 degrees C preparations (alpha-42 sera). Western blot analysis revealed that alpha-WCP sera in part reacted with different proteins when compared with alpha-MAP sera. Furthermore, some proteins were only detected by alpha-32 but not by alpha-42 sera. In conclusion, the results demonstrate the immunogenicity of cell wall MAP of S. suis, and highlight the importance of considering growth conditions in the preparation of subunit vaccines.
Institut für Mikrobiologie, Zentrum für Infektionsmedizin, Bischofsholer Damm 15, 30173 Hannover, Germany.
L
L
Benga
R
R
Goethe
E
E
Grosse Beilage
P
P
Valentin-Weigand
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006358:Hot Temperature; D010457:Peptidoglycan / Q000276:immunology*; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary*; D017011:Streptococcus suis / Q000145:classification / Q000276:immunology*; D013312:Stress, Physiological; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology*
D000907:Antibodies, Bacterial; D010457:Peptidoglycan