Albumin loaded microsphere of amphiphilic poly(ethylene glycol)/ poly(alpha-ester) multiblock copolymer.
The purpose of this study is to investigate the microspheres (MS) based on (AB)(n) type amphiphilic multiblock copolymers for sustained and complete release of a model protein, bovine serum albumin (BSA). The MS were prepared by a modified water-in-oil-in-water (W/O/W) double emulsion method using amphiphilic multiblock copolymers consisting of poly(ethylene glycol) (PEG) and a poly(alpha-ester), poly(epsilon-caprolactone) (PCL) or poly(l-lactic acid) (PLLA). The size of MS and encapsulation efficiency of BSA within MS were not noticeably influenced by the copolymer composition used in this experiment. While BSA was completely released from PEG/PLLA MS through matrix erosion and the diffusion of BSA, it was released only to an extent of 60% from PEG/PCL MS solely through the diffusion process. However, the release of BSA from PEG/PCL MS dramatically increased and then reached 100% release in 10 days after thermal treatment of the MS at 50 degrees C for 30 min in the middle of release test (on day 15).
Center for Biomaterials and Biotechnology, Department of Materials and Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Korea.
Jong-Ho
JH
Kim
You Han
YH
Bae
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D005027:Ethylene Oxide / Q000737:chemistry*; D006358:Hot Temperature; D007773:Lactates / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D007773:Lactates; D007783:Lactones; C400421:PLC(20)-b-PEO(44); D013501:Surface-Active Agents; C088513:poly(lactic acid-ethylene glycol); D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D005027:Ethylene Oxide