Ischemic preconditioning-induced expression of gp130 and STAT3 in astrocytes of the rat hippocampus.
We investigated the distribution and time course of expression of both gp130 mRNA and signal transducer and activator of transcription factor-3 (STAT3) in a rat model of ischemic tolerance induction. Forebrain ischemia was induced by four-vessel occlusion for 3 min as an ischemic preconditioning. Ischemic preconditioning 3 days before a 10-min lethal ischemia preserved neuronal signal. Expression of gp130 mRNA was induced 12 h after ischemic preconditioning, and was most prominent in the CA1 and the hilar region at 3 days, with expression sustained for at least 7 days. Ischemic preconditioning-induced STAT3 activation, as revealed by nuclear translocation, resembled that of gp130 expression. Nuclear STAT3 immunoreactivity occurred in the CA1 and the hilar region within 12 h after ischemic preconditioning, and was sustained for at least 7 days. Double-labeling experiments revealed that the cells expressing gp130 or STAT3 were glial fibrillary acidic protein (GFAP) immunoreactive astrocytes. These results demonstrate upregulation of gp130 and STAT3 in reactive astrocytes following ischemic preconditioning, indicating that this signal pathway is involved in the astroglial reaction to ischemic preconditioning in the rat hippocampus.
Kim
Seong Yun
SY
Department of Pharmacology, College of Medicine, The Catholic University of Korea, 137-701 Seoul, South Korea.
Park
Hyun-Jung
HJ
Choi
Jeong-Sun
JS
Lee
Ju Eun
JE
Cha
Jung-Ho
JH
Choi
Yun-Sik
YS
Cho
Kyung-Ok
KO
Chun
Myung-Hoon
MH
Lee
Mun-Yong
MY
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism; D050822:Cytokine Receptor gp130; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D017403:In Situ Hybridization; D019194:Ischemic Preconditioning; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D004268:DNA-Binding Proteins; C494201:Il6st protein, rat; D008562:Membrane Glycoproteins; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators; D050822:Cytokine Receptor gp130