Occurrence of Gluconacetobacter diazotrophicus in tropical and subtropical plants of Western Ghats, India.
Endophytic bacteria were isolated from the tissues of surface sterilized roots, stems, and leaves of fifty different crop plants. Phenotypic, biochemical tests and species-specific PCR assay permitted identification of four isolates of Gluconacetobacter diazotrophicus from root tissues of carrot (Daucus carota L.), raddish (Raphanus sativus L.), beetroot (Beta vulgaris L.) and coffee (Coffea arabica L.). Further the plant growth promoting traits such as nitrogenase activity, production of phytohormone indole acetic acid (IAA), phosphorus and zinc solubilization were assessed. Significant nitrogenase activity was recorded among the isolates and all the isolates produced IAA in the presence of tryptophan. Though all the four isolates efficiently solubilized phosphorus, the zinc solubilizing ability differed among the isolates.
Madhaiyan
M
M
Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea. mmadhaiyan@hotmail.com
Saravanan
V S
VS
Jovi
D Bhakiya Silba Sandal
DB
Lee
Hyoungseok
H
Thenmozhi
R
R
Hari
K
K
Sa
Tongmin
T
D003069:Coffee / Q000382:microbiology*; D018556:Crops, Agricultural / Q000382:microbiology*; D041821:Gluconacetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007194:India; D007210:Indoleacetic Acids / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism; D010758:Phosphorus; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014329:Tropical Climate; D014675:Vegetables / Q000382:microbiology*; D015032:Zinc
D003069:Coffee; D007210:Indoleacetic Acids; D010758:Phosphorus; C030737:indoleacetic acid; D009591:Nitrogenase; D015032:Zinc