Phylogenetic analysis of a human group B rotavirus WH-1 detected in China in 2002.
A human group B rotavirus strain WH-1 was detected in an adult sporadic case of diarrhea in Wuhan, China in 2002. In this study, the gene sequences of WH-1 were determined in order to examine the phylogenetic relatedness to other human group B rotaviruses found previously in China (ADRV, in 1982), India (CAL-1, in 1998), and Bangladesh (Bang373, in 2000), as well as animal viruses, and to estimate the mutation rate of group B rotavirus. VP7 (major outer capsid protein) gene of WH-1 showed extremely high sequence identity (98.6%) to ADRV and showed relatively high sequence identities to CAL-1 (92.5%) and Bang373 (92.4%). In contrast, identities to animal (bovine and murine) group B rotaviruses were considerably lower (61-64%). Other gene segments of WH-1 encoding VP2, VP4, VP6, NSP1-NSP3, and NSP5 also showed high sequence identities to ADRV genes (98-99%), which were generally higher than those to CAL-1 genes and Bang373 genes (90-95%). However, amino acid sequence identities between WH-1 and ADRV were almost the same (VP2, VP6, and NSP3), or lower (NSP2) than those between WH-1 and CAL-1 (or Bang373). Since rates of synonymous substitution and transition between WH-1 and ADRV were similar for all the segments analyzed, genetic evolution was considered to have occurred neutrally and at a similar speed in most of the RNA segments. Based on the sequence divergence between WH-1 and ADRV, the mutation rate in natural condition of human group B rotavirus was estimated as 7.9 x 10(-4) substitution/site per year. The frequency of synonymous substitution between ADRV and Bang373 was 5.7 times higher than that between ADRV and WH-1, suggesting that the group B rotaviruses of Indian-Bangladeshi lineage diverged from that of Chinese lineage several decades ago.
Yang
Ji-Hong
JH
Wuhan Centers for Disease Prevention and Control, Wuhan, China.
Kobayashi
Nobumichi
N
Wang
Yuan-Hong
YH
Zhou
Xuan
X
Li
Yan
Y
Zhou
Dun-Jin
DJ
Hu
Zhi-Hong
ZH
Ishino
Masaho
M
Alam
Mohammed M
MM
Naik
Trailokya N
TN
Ahmed
Muzahed U
MU
D000328:Adult; D036022:Capsid Proteins / Q000235:genetics; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D017421:Sequence Analysis
D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus