Dual role of the adenovirus pVI C terminus as a nuclear localization signal and activator of the viral protease.
Adenain, the protease produced by adenovirus, is regulated by formation of a heterodimer with an 11 aa peptide derived from the C terminus of another adenoviral protein, pVI. Here, the role of the basic motif KRRR, which is conserved in pVI sequences from human adenovirus serotypes, was investigated. It was shown that this motif is less important than the N- or C-terminal regions in the formation of the adenain-peptide heterodimer and in the activity of the subsequent complex. This motif, however, acted as a nuclear localization signal that was capable of targeting heterologous proteins to the nucleus, resulting in a distinctive intranuclear distribution consisting of discrete foci, which is similar to that found for pVI during adenovirus infection.
Honkavuori
K S
KS
Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK.
Pollard
B D
BD
Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK.
Rodriguez
M S
MS
Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK.
Hay
R T
RT
Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK.
Kemp
G D
GD
Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK.
D000260:Adenoviruses, Human / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment; D015398:Signal Transduction; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D010446:Peptide Fragments; D014764:Viral Proteins; C105093:glycyl-valyl-glutaminyl-seryl-leucyl-lysyl-arginyl-arginyl-arginyl-cysteinyl-phenylalanine; D003546:Cysteine Endopeptidases; C091827:adenain