[Discovery of genes active in embryogenesis by gene trapping].
Gene trapping is one of the most promising technologies of vector mutagenesis used for discovery of genes active in embryogenesis. A brief description is provided for gene trapping based on the use of embryonic stem cells and vectors carrying the reporter gene lacZ without promoter, as well as the results obtained with the help of this technology. A total of four transgenic mouse lines were obtained, in three of which the vector was integrated into genes active during embryogenesis. As a result, various patterns of beta-galactosidase expression were observed in the limb rudiments, heart, liver, and other organs at different embryonic stages. At present, detailed studies of the site and time of action of the mutated genes in embryonic and postnatal development are under way, as well as molecular-genetic identification of these genes.
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Fedorov
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D001616:beta-Galactosidase