Steric effects of isoleucine 107 on heme reorientation reaction in human myoglobin.
Structural factors to regulate the heme reorientation reaction in myoglobin were examined and we found that the side chain at position 107 (Ile107), which is located between the 2-vinyl and 3-methyl groups of heme, forms a kinetic barrier for the heme rotation about the alpha-gamma axis. The phenylalanine-substituted mutant showed an extremely slow heme reorientation rate, compared to that of the wild-type protein, while replacement by the decreased side chain, valine, at position 107 accelerated the reorientation reaction. Considering that the spectroscopic data show only minor structural changes in the heme environments of the Ile107 mutants, the side chain at position 107 sterically interacts with the heme peripheral groups in the activation state for the heme reorientation, which supports the intramolecular mechanism that the heme rotates about the alpha-gamma axis without leaving the "protein cage."
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Haruto
H
Ishikawa
Satoshi
S
Takahashi
Koichiro
K
Ishimori
Isao
I
Morishima
D001671:Biochemistry / Q000379:methods; D006418:Heme / Q000737:chemistry; D006427:Hemin / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007532:Isoleucine / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D010649:Phenylalanine / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman / Q000379:methods; D013237:Stereoisomerism; D014965:X-Rays
D009211:Myoglobin; D011506:Proteins; D007532:Isoleucine; D006418:Heme; D010649:Phenylalanine; D006639:Histidine; D006427:Hemin