[Induction of HSF1 expression and sporadic colorectal cancer].
To explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers.



A gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT-PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer.



The expression of hsf1, hsf27 and inos was increased in colon cancer compared with normal colon mucosa using PathwayFinder GEArray. The RT-PCR results showed that the expression of hsf1 was detected in 86% of patients(30/35)and the expression of inos detected in 63% patients(22/35).



Hsf1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.
Fang
Yong-ming
YM
The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Dong
Qi
Q
Cen
Hui
H
Tang
Xiao-ping
XP
Zheng
Shu
S
D018797:Cell Cycle Proteins; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D000076249:Heat Shock Transcription Factors; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics
C405928:CDK5RAP3 protein, human; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins