Transepithelial electrical measurements with the Ussing chamber.
The Ussing chamber technique is a simple, but powerful technique to investigate ion transport. Originally designed to study vectorial ion transport through the frog skin, it has revolutionized our knowledge about how eletrolytes permeate epithelia. Here we discuss the physiological principles that underlie the technique and protocols to investigate the role of the cystic fibrosis transmembrane conductance regulator (CFTR) in transepithelial ion transport.
University of Bristol, Department of Physiology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK.
Hongyu
H
Li
David N
DN
Sheppard
Martin J
MJ
Hug
D002462:Cell Membrane / Q000502:physiology*; D002478:Cells, Cultured; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology; D004594:Electrophysiology / Q000295:instrumentation*; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D017136:Ion Transport / Q000502:physiology*; D017736:Ion-Selective Electrodes; D008564:Membrane Potentials / Q000502:physiology; D011199:Potentiometry / Q000295:instrumentation
C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator