Reconstitution of human airway tissue in the humanized xenograft model.
Normal human airway epithelial tissue may be reconstituted in the humanized xenograft model in immunodeficient NUDE mice. Epithelial cells dissociated from human fetal or adult tissue are seeded on a denuded rat trachea and implanted in the NUDE mice. After a first step of dedifferentiation, the human epithelial cells adhere on the denuded basal lamina of the rat host trachea and progressively reconstitute a normal well-differentiated epithelium after several steps of migration, proliferation, stratification and redifferentiation.
INSERM UMR-S 514-CHU Maison Blanche, 45, rue Cognacq Jay, 51092 Reims, France.
Sandie
S
Escotte
Corinne
C
Catusse
Christelle
C
Coraux
Edith
E
Puchelle
D000818:Animals; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena; D017690:Cell Transplantation / Q000379:methods*; D004847:Epithelial Cells / Q000502:physiology / Q000637:transplantation*; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000502:physiology / Q000637:transplantation*; D014132:Trachea / Q000166:cytology / Q000503:physiopathology; D014133:Tracheal Diseases / Q000628:therapy*; D014183:Transplantation, Heterologous