Expression profile of mRNAs from rat hippocampus and its application to microarray.
Stress refers to physiological or psychological stimuli that disrupt homeostasis and induce pathophysiological conditions due to maladaptive response, sometimes resulting in mental disorders including depression and post-traumatic stress disorder. Severe stress has been shown to induce neuronal atrophy and apoptosis, especially in the hippocampus, which is thought to be a region of the brain important in stress-related disorders. We have analyzed gene expression in rat hippocampus comprehensively to clarify the molecular mechanism of stress-related disorders. In the present study, we identified and catalogued 13,660 partial complementary DNA sequences (expressed sequence tags (ESTs)) of randomly selected clones from a cDNA library of rat hippocampus. Sequence analysis showed that these clones cluster into 7173 non-redundant sequences comprising 1794 clusters and 5379 singletons. As a result of nucleotide and peptide database search, 2594 were found to represent known rat sequences. Of the remaining 4579 genes, 599 non-redundant ESTs represent rat homologs of genes identified in other species or new members of structurally related families. In addition, we illustrate the use of these clone sets by constructing a cDNA microarray focused on genes categorized into "cell/organism defense". These ESTs and our own microarray thus provide an improved genomic source for molecular studies of animal models of stress-related disorders.
Tanaka
Takeshi
T
Department of Psychiatry and Human Behavior, Gunma University, Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan.
Horikawa
Yukio
Y
Kawamoto
Takanori
T
Kabe-Sakurai
Noriko
N
Takeda
Jun
J
Mikuni
Masahiko
M
D000818:Animals; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D006624:Hippocampus / Q000502:physiology*; D006706:Homeostasis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D012044:Regression Analysis; D013315:Stress, Psychological / Q000235:genetics