Protective effect of nicotine on tunicamycin-induced apoptosis of PC12h cells.
Nicotine has been reported to have neuroprotective effects. The present study deals with the neuroprotective effect of nicotine on the tunicamycin-induced apoptosis of PC12h cells. Treatment of PC12h cells with tunicamycin causes endoplasmic reticulum stress leading to apoptosis. Nicotine dose-dependently prevented the tunicamycin-induced apoptosis. Hoechst 33258 staining demonstrated the protective effect of nicotine against tunicamycin-induced apoptosis. Treatment with nicotinic acetylcholine receptor (nAChR) and L-type voltage-sensitive calcium channel (L-VSCC) antagonists prevented the nicotine-induced protective effect. A phosphatidylinositol 3-kinase (PI3-K) inhibitor had no influence on the nicotine-induced neuroprotective effect. These results show that the neuroprotective effect of nicotine occurs through nAChRs including the alpha 7 subtype and L-VSCC in PC12h cells and not through the PI3-K/Akt pathway.
Utsumi
Takahiro
T
Laboratory of Neurobiology, Faculty of Engineering and High Technology Research Center, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
Shimoke
Koji
K
Kishi
Soichiro
S
Sasaya
Harue
H
Ikeuchi
Toshihiko
T
Nakayama
Hitoshi
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001562:Benzimidazoles; D002470:Cell Survival / Q000187:drug effects; D018680:Cholinergic Antagonists / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors; D009025:Morpholines / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D014415:Tunicamycin / Q000037:antagonists & inhibitors / Q000633:toxicity*
D001562:Benzimidazoles; D018680:Cholinergic Antagonists; D002867:Chromones; D004791:Enzyme Inhibitors; D009025:Morpholines; D018722:Nicotinic Agonists; C447680:hoechst 32258; D014415:Tunicamycin; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D009538:Nicotine