Human serum provided additional values in growth factors supplemented medium for human chondrocytes monolayer expansion and engineered cartilage construction.
We have previously formulated an optimized human chondrocytes growth medium based on 2% fetal bovine serum supplementation. For clinical usage, the animal serum must be replaced by patient own serum. We investigated the effects of human serum concentration for human nasal septum chondrocytes monolayer culture and cartilage reconstruction. Human serum demonstrated a dose dependent manner in promoting chondrocytes growth and cartilage engineering.
Chua
K H
KH
Department of Physiology, Medical Faculty of Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Fuzina
N H
NH
Ruszymah
B H I
BH
D000818:Animals; D002356:Cartilage / Q000637:transplantation*; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology*; D024042:Collagen Type I / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics; D017077:Culture Media, Conditioned; D015870:Gene Expression / Q000502:physiology; D006133:Growth Substances / Q000097:blood*; D006801:Humans; D009300:Nasal Septum; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D024043:Collagen Type II; D017077:Culture Media, Conditioned; D006133:Growth Substances