High-performance liquid chromatographic analysis of dextromethorphan, guaifenesin and benzoate in a cough syrup for stability testing.
A method has been developed for the analysis of a cough syrup containing dextromethorphan, guaifenesin, benzoic acid, saccharin and other components. Forced degradation was also studied to demonstrate that the method could be employed during a stability study of the syrup. Final conditions were phosphate buffer (25 mM, pH 2.8) with triethylamine (TEA)-acetonitrile (75:25, v/v). In such conditions, all the actives, excipients and degradation products were baseline resolved in less than 14 min, and different wavelengths were used for the different analytes and related compounds.
Galli
V
V
Sección Química Analítica, Facultad de CC Experimentales y de la Salud, Universidad San Pablo-CEU, Urbanización Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.
Barbas
C
C
D000097:Acetonitriles / Q000737:chemistry; D000996:Antitussive Agents / Q000737:chemistry* / Q000627:therapeutic use; D001565:Benzoates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003371:Cough / Q000188:drug therapy; D003915:Dextromethorphan / Q000032:analysis*; D004355:Drug Stability; D005021:Ethylamines / Q000737:chemistry; D006140:Guaifenesin / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012439:Saccharin / Q000032:analysis
D000097:Acetonitriles; D000996:Antitussive Agents; D001565:Benzoates; D005021:Ethylamines; D010710:Phosphates; D006140:Guaifenesin; D003915:Dextromethorphan; D012439:Saccharin; C016162:triethylamine; C032159:acetonitrile