The monosodium iodoacetate model of osteoarthritis: a model of chronic nociceptive pain in rats?
Osteoarthritis (OA) is a widespread condition affecting the elderly population. One of the most prominent features but least studied symptoms is chronic pain associated with OA. The study objective was to determine pain endpoints in rats with monosodium iodoacetate (MIA) induced OA, and to investigate the efficacy of common nociceptive agents. Sprague-Dawley rats received an intraarticular injection of either 25 microl 80 mg/ml MIA or 25 microl 0.9% sterile saline into the right knee joint. Changes in von Frey thresholds and latencies to stroking with a cotton bud (punctate and dynamic allodynia, respectively) were measured pre- and for up to 10 weeks post-intraarticular injection. Changes in hind paw weight distribution were also determined. Both punctate allodynia and a weight bearing deficit were observed in MIA-treated rats for up to 10 weeks. Interestingly, dynamic allodynia was not detected at any time point tested. Morphine (0.3-3 mg/kg, s.c.) and tramadol (3-100 mg/kg, p.o.) dose-dependently inhibited punctate allodynia and partially reversed weight bearing deficit. In conclusion, the MIA model of OA is reproducible and mimics OA pain in humans. Analgesic drug studies indicate this model may be useful for investigating chronic nociceptive pain.
Pain Therapeutics, Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent CT139NJ, UK. Rachel.Combe@Pfizer.com
Rachel
R
Combe
Steve
S
Bramwell
Mark J
MJ
Field
D000704:Analysis of Variance; D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors; D007839:Functional Laterality; D006930:Hyperalgesia / Q000503:physiopathology; D007461:Iodoacetates; D008297:Male; D009020:Morphine / Q000627:therapeutic use; D010003:Osteoarthritis / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology; D010146:Pain / Q000188:drug therapy / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D016474:Weight-Bearing / Q000502:physiology
D004791:Enzyme Inhibitors; D007461:Iodoacetates; D009020:Morphine