Production of maltodextrin 1-phosphate by Fibrobacter succinogenes S85.
We show for the first time the occurrence of maltodextrin-1-Phosphate (MD-1P) (DP2) in F. succinogenes S85, a rumen bacterium specialized in cellulolysis which is not able to use maltose and starch. MD-1P were found in intra and extracellular medium of resting cells incubated with glucose. We used 2D 1H NMR technique and TLC to identify their structure and quantify their production with time. It was also shown that these phosphorylated oligosaccharides originated both from exogenous glucose and endogenous glycogen.
Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal--CNRS, 63177 Aubière cedex, France.
Régis
R
Nouaille
Maria
M
Matulova
Anne-Marie
AM
Delort
Evelyne
E
Forano
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D045848:Fibrobacter / Q000201:enzymology* / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000096:biosynthesis*
D001426:Bacterial Proteins; D011134:Polysaccharides; C008315:maltodextrin