Occurrence of D-histidine residues in antimicrobial poly(arginyl-histidine), conferring resistance to enzymatic hydrolysis.
The antimicrobial peptide poly(arginyl-histidine) is secreted by the ergot fungus Verticillium kibiense. We previously showed that poly(arginyl-histidine) from the fungus inhibits the growth of certain microorganisms more effectively than that chemically synthesized from the L-form of arginine and histidine, implying some substantial differences between the fungal and synthetic peptides. To elucidate what causes such differences, we here investigated the structural features of the fungal peptides. The acid hydrolysates of the fungal peptide contained d-histidine. When synthetic poly(L-arginyl-D-histidine) mimicking the fungal peptide was added to the culture of Salmonella typhimurium together with poly(L-arginyl-L-histidine), poly(L-arginyl-D-histidine) was not easily degraded during the incubation compared with poly(L-arginyl-L-histidine). We concluded that the d-form of histidine residues in the fungal peptide prolongs the life of the peptide leading to the enhancement of antimicrobial activity.
Research Institute for Biological Sciences Okayama (RIBS), 7549-1 Kayo-cho, Okayama 716-1241, Japan. mnishikawa@bio-ribs.com
Masanobu
M
Nishikawa
Ken'ichi
K
Ogawa
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D006639:Histidine / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D020172:Verticillium / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D010455:Peptides; C481666:poly(arginyl-histidine); D006639:Histidine