Ice-water interface migration by temperature controlling for stretching of DNA molecules.
This report shows a new DNA stretching method using migration of an ice-water interface. DNA molecules were stretched accompanying the migration of the solid-liquid interface and immobilized in frozen area. This simple method needs no chemical modification to keep DNA in the stretched form. For full stretching of DNA molecules, one terminus of the DNA molecules were anchored on silanized substrate. The anchored DNA molecules were stretched by freezing the DNA solution. The stretched DNA molecules were observed after sublimation of the frozen solution keeping its stretched form on silanized surface which had no attractive interaction with DNA molecules except for the SH-modified terminus in solution. An infrared (IR) laser beam was introduced to a frozen DNA solution through an objective lens for local area melting of the solution. Scanning of the laser irradiation caused stretching and enclosing of DNA molecules in the frozen area followed by migration of the solid-liquid interface.
Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, 441-8580, Japan.
J
J
Komatsu
M
M
Nakano
H
H
Kurita
T
T
Takashima
S
S
Katsura
A
A
Mizuno
D055592:Biophysical Phenomena; D001703:Biophysics; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D005898:Glass; D007053:Ice; D007259:Infrared Rays; D009690:Nucleic Acid Conformation; D012821:Silanes / Q000737:chemistry; D013696:Temperature; D014867:Water
D007053:Ice; D012821:Silanes; D014867:Water; C099469:dichlorosilane; D004247:DNA