Reduced and high molecular weight barley beta-glucans decrease plasma total and non-HDL-cholesterol in hypercholesterolemic Syrian golden hamsters.
Consumption of concentrated barley beta-glucan lowers plasma cholesterol because of its soluble dietary fiber nature. The role of molecular weight (MW) in lowering serum cholesterol is not well established. Prior studies showed that enzymatic degradation of beta-glucan eliminates the cholesterol-lowering activity; however, these studies did not evaluate the MW of the beta-glucan. The current study was conducted to evaluate whether barley beta-glucan concentrates, partially hydrolyzed to reduce MW, possess cholesterol-lowering and antiatherogenic activities. The reduced MW fraction was compared with a high MW beta-glucan concentrate from the same barley flour. Concentrated beta-glucan preparations were evaluated in Syrian Golden F(1)B hamsters fed a hypercholesterolemic diet (HCD) with cholesterol, hydrogenated coconut oil, and cellulose. After 2 wk, hamsters were fed HCD or diets that contained high or reduced MW beta-glucan at a concentration of 8 g/100 g at the expense of cellulose. Decreases in plasma total cholesterol (TC) and non-HDL-cholesterol (non-HDL-C) concentrations occurred in the hamsters fed reduced MW and high MW beta-glucan diets. Plasma HDL-C concentrations did not differ. HCD-fed hamsters had higher plasma triglyceride concentrations. Liver TC, free cholesterol, and cholesterol ester concentrations did not differ. Aortic cholesterol ester concentrations were lower in the reduced MW beta-glucan-fed hamsters. Consumption of either high or reduced MW beta-glucan increased concentrations of fecal total neutral sterols and coprostanol, a cholesterol derivative. Fecal excretion of cholesterol was greater than in HCD-fed hamsters only in those fed the reduced MW beta-glucan. Study results demonstrate that the cholesterol-lowering activity of barley beta-glucan may occur at both lower and higher MW.
Wilson
Thomas A
TA
Center for Health and Disease Research, Department of Health and Clinical Sciences, University of Massachusetts-Lowell, Lowell, MA 01854, USA.
Nicolosi
Robert J
RJ
Delaney
Bryan
B
Chadwell
Kim
K
Moolchandani
Vikas
V
Kotyla
Timothy
T
Ponduru
Sridevi
S
Zheng
Guo-Hua
GH
Hess
Richard
R
Knutson
Nathan
N
Curry
Leslie
L
Kolberg
Lore
L
Goulson
Melanie
M
Ostergren
Karen
K
D000818:Animals; D002784:Cholesterol / Q000097:blood*; D006224:Cricetinae; D004043:Dietary Fiber / Q000627:therapeutic use*; D005243:Feces / Q000737:chemistry; D005936:Glucans / Q000008:administration & dosage / Q000627:therapeutic use*; D001467:Hordeum; D006937:Hypercholesterolemia / Q000188:drug therapy*; D008297:Male; D008647:Mesocricetus; D008970:Molecular Weight
D004043:Dietary Fiber; D005936:Glucans; D002784:Cholesterol