Evaluating chick Galpha-interacting protein selectivity.
Regulators of G-protein signaling (RGS) proteins constitute a large family of GTPase-accelerating proteins (GAPs) for heterotrimeric G proteins. More than 30 RGS genes have been identified in mammals. One of these, the Galpha-interacting protein (GAIP), interacts preferentially with members of the G(i/o) subfamily of G-protein alpha subunits in mammalian cells. A unique isoform of GAIP, derived from embryonic chicken dorsal root ganglion neurons, has a short N terminus that is only 41% identical to known mammalian orthologs. Consistent with this unique primary structure, chick GAIP has higher target specificity than its mammalian counterparts. This article describes both in vitro and in vivo methods used to characterize chick GAIP selectivity.
Institut de Neurobiologie de la Mediterranée, INMED/INSERM U29, Marseille, France.
Patrizia
P
Tosetti
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002642:Chick Embryo; D004594:Electrophysiology; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D020710:RGS Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D010750:Phosphoproteins; D020033:Protein Isoforms; D021122:Protein Subunits; D020710:RGS Proteins; D011994:Recombinant Proteins; C097187:regulator of G-protein signalling 19; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium