MSMA resistance studies.
Monosodium methanearsonate (MSMA)-resistant and -susceptible common cocklebur (Xanthium strumarium L.) and cotton (Gossypium hirsutum L.) were treated with MSMA. Plant parameters analyzed were: glutathione synthetase activity, selected amino acid (arginine, glutamic acid, alanine, citrulline, glutamine, and glutathione) content and arsenic content (MSMA, total arsenic, and arsonate). No reduction of arsenic from the parent pentavalent form present in MSMA to the trivalent form was detected. Arginine, glutamic acid, and glutamine content increased in tissue three days after MSMA treatment. Glutathione content decreased during the first three days after treatment; however, five days after treatment the resistant biotype of cocklebur and cotton had elevated glutathione levels (8-20 times greater, respectively). Glutathione Synthetase activity was higher in cotton than in either of the cocklebur biotypes; MSMA did not affect its activity in cotton or either cocklebur biotype. Resistant biotypes have a slightly higher activity than the susceptible biotype. Tolerance of cotton to MSMA may be related to glutathione synthetase activity and possibly to the presence of phytochelatins. Also, increased glutathione levels in the resistant biotype may implicate phytochelatin involvement in the resistance mechanism.
Department of Entomology, Soils, and Plant Sciences, Clemson University, Clemson, South Carolina 29634-0315, USA. dcamper@clemson.edu
N D
ND
Camper
R J
RJ
Keese
P S
PS
Coker
D000222:Adaptation, Physiological; D001152:Arsenicals / Q000494:pharmacology*; D017753:Ecosystem; D005978:Glutathione / Q000032:analysis; D003368:Gossypium / Q000502:physiology; D006540:Herbicides / Q000494:pharmacology*; D007305:Insecticide Resistance; D031210:Xanthium / Q000502:physiology
D001152:Arsenicals; D006540:Herbicides; D005978:Glutathione; C020300:monomethylarsonic acid