Biochemical brain markers and purinergic parameters in rat CSF after seizure induced by pentylenetetrazol.
Cellular and molecular mechanisms involved in the generation of seizures and the magnitude of neural cells injury are not fully understood. We evaluated astrocyte and/or neuronal injury in rats in the pentylenetetrazol model of acute seizures by measuring S100B and NSE levels in cerebrospinal fluid. Additionally, we determined ADP and GDP hydrolysis by soluble nucleoside triphosphate diphosphohydrolase in the cerebrospinal fluid, and the concentration of nucleosides adenosine, inosine and guanosine as putative markers of brain injury. After pentylenetetrazol-induced seizures: (i) S100B values increased from 10 to 30 min, returning to control levels at 24 h; NSE levels presented a biphasic increase: an increase at 10 to 30 min returning to control levels, and again at 240 min followed by a decline at 24 h; (ii) nucleotidase activities increased from 10 min, returning to control levels at 240 min; (iii) guanosine and inosine levels increased exclusively after 30 min. In summary, this study showed biochemical changes in the cerebrospinal fluid occurring after seizures induced by pentylenetetrazol. Such events may have a modulating effect upon seizure expression, particularly nucleoside triphosphate diphosphohydrolase activities and nucleoside concentrations, but are nevertheless followed by neural death as evidenced by the increase in NSE and S100B levels.
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600 anexo, 90035-003, Porto Alegre, RS, Brazil.
Jean Pierre
JP
Oses
Renata
R
Leke
Luis Valmor
LV
Portela
Diogo R
DR
Lara
André P
AP
Schmidt
Emerson André
EA
Casali
Susana
S
Wofchuk
Diogo O
DO
Souza
João José Freitas
JJ
Sarkis
D000241:Adenosine / Q000134:cerebrospinal fluid; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000134:cerebrospinal fluid; D001081:Apyrase / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D003292:Convulsants; D004195:Disease Models, Animal; D005260:Female; D006151:Guanosine / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000378:metabolism; D006868:Hydrolysis; D007288:Inosine / Q000494:pharmacology; D009410:Nerve Degeneration / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000134:cerebrospinal fluid; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid*; D010433:Pentylenetetrazole; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid; D011687:Purines / Q000134:cerebrospinal fluid*; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000134:cerebrospinal fluid; D012640:Seizures / Q000134:cerebrospinal fluid* / Q000139:chemically induced / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D015703:Antigens, CD; D015415:Biomarkers; D003292:Convulsants; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011687:Purines; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; D006151:Guanosine; D006153:Guanosine Diphosphate; D007288:Inosine; D000244:Adenosine Diphosphate; D001081:Apyrase; C067869:CD39 antigen; D010751:Phosphopyruvate Hydratase; D000241:Adenosine; D010433:Pentylenetetrazole