[Establishment of CPP-SOM integrated cDNA microarray technology].
OBJECTIVE
To get an insight into the molecular mechanisms of diseases development and targeted therapy at the transcriptome level and search for potential therapeutic targets.


METHODS
The present researchers established a cDNA microarray platform and applied component plane presentation integrated self-organizing map (CPP-SOM) to the microarray data obtained from a differentiation model, all trans retinoic acid-induced differentiation in NB4 cells.


RESULTS
The platform included 12630 unique clones, including 9436 known genes. By CPP-SOM, the researchers were able to not only well classify the regulated genes into functionally distinct categories but also depict transcriptional changes throughout the process of the development of diseases or drug treatment.


CONCLUSIONS
The platform has proven to be steady and reliable, and the CPP-SOM could serve as an important and good tool for analysis of microarray data.
State Key Lab for Medical Genomics, Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Second Medical University, Shanghai, 200025 P.R. China.
Pei-zheng
PZ
Zheng
Chun-jun
CJ
Zhao
Yan-zhi
YZ
Du
Sai-juan
SJ
Chen
Zhu
Z
Chen
Ji
J
Zhang
Qing-hua
QH
Zhang
Kan-kan
KK
Wang
D045744:Cell Line, Tumor; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction