Diagnosis of taenia saginata cysticercosis by immunohistochemical test on formalin-fixed and paraffin-embedded bovine lesions.
A new method of diagnosing cysticercus or larval stage of the human tapeworm, Taenia saginata, also known as Cysticercus bovis, in formalin-fixed bovine tissue was developed using a monoclonal antibody to T. saginata and avidin-biotin complex immunohistochemistry. Grossly recognizable viable and degenerate cysts were identifiable after immunohistochemical staining and could be differentiated from Sarcocystis, Actinobacillus, or non-cyst, normal bovine structures. Thenew test should permit laboratory confirmation of suspected T. saginata cysticercus lesions.
Ogunremi
Oladele
O
Centre for Animal Parasitology, Canadian Food Inspection Agency, Saskatoon Laboratory, Saskatoon, Saskatchewan, Canada.
MacDonald
Garry
G
Geerts
Stanny
S
Brandt
Jef
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology*; D003551:Cysticercosis / Q000175:diagnosis* / Q000469:parasitology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D041202:Taenia saginata / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal