Preparation and characterization of biodegradable microspheres containing hepatitis B surface antigen.
Poly-DL-lactide-poly(ethylene glycol) (PELA) microspheres containing Hepatitis B surface antigen (HBsAg) were elaborated by a solvent extraction method based on the formation of a double water/oil/water (w/o/w) emulsion. Microspheres were characterized in terms of morphology, size and size distribution, encapsulation efficiency, and the efficiency of microsphere formation (EMF). Transmission electron microscopy (TEM) and polyacrylamide gel electrophoresis (PAGE) were used to investigate the structural integrality of HBsAg encapsulated in PELA microspheres. The release profile was investigated by the measurement of antigen present in the release medium at various intervals. The PELA-10 microspheres displayed the highest antigen encapsulation efficiency (about 80%), and antigen molecules could be stabilized in the PELA-10 microspheres during the preparation process. It suggested that the PELA microspheres had a great potential as a new polymer adjuvant for HBsAg. The release of Hepatitis B surface antigen from poly-DL-lactide-poly(ethylene glycol) microspheres.
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, P. O. Box 415, China. shaobingzhou@163.net
Shaobing
S
Zhou
Xueyan
X
Liao
Zhenglun
Z
Liang
Xiaohong
X
Li
Xianmo
X
Deng
Heming
H
Li
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis* / Q000737:chemistry*; D001673:Biodegradation, Environmental; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000737:chemistry*; D008863:Microspheres; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry
D000276:Adjuvants, Immunologic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006514:Hepatitis B Surface Antigens; D011091:Polyesters; D011108:Polymers; D011092:Polyethylene Glycols; C033616:poly(lactide)