PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum.
Protein disulfide isomerase (PDI) is the archetypal enzyme involved in the formation and reshuffling of disulfide bonds in the endoplasmic reticulum (ER). PDI achieves its redox function through two highly conserved thioredoxin domains, and PDI can also operate as an ER chaperone. The substrate specificities and the exact functions of most other PDI family proteins remain important unsolved questions in biology. Here, we characterize a new and striking member of the PDI family, which we have named protein disulfide isomerase-like protein of the testis (PDILT). PDILT is the first eukaryotic SXXC protein to be characterized in the ER. Our experiments have unveiled a novel, glycosylated PDI-like protein whose tissue-specific expression and unusual motifs have implications for the evolution, catalytic function, and substrate selection of thioredoxin family proteins. We show that PDILT is an ER resident glycoprotein that liaises with partner proteins in disulfide-dependent complexes within the testis. PDILT interacts with the oxidoreductase Ero1alpha, demonstrating that the N-terminal cysteine of the CXXC sequence is not required for binding of PDI family proteins to ER oxidoreductases. The expression of PDILT, in addition to PDI in the testis, suggests that PDILT performs a specialized chaperone function in testicular cells. PDILT is an unusual PDI relative that highlights the adaptability of chaperone and redox function in enzymes of the endoplasmic reticulum.
van Lith
Marcel
M
Department of Biological Sciences, University of Durham, Durham, DH1 3LE, United Kingdom.
Hartigan
Nichola
N
Hatch
Jennifer
J
Benham
Adam M
AM
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D045744:Cell Line, Tumor; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D006031:Glycosylation; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007535:Isomerases / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D018832:Molecular Chaperones / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013737:Testis / Q000201:enzymology*
D004220:Disulfides; D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; C062477:endoplasmic reticulum glycoprotein p72; C405327:ERO1A protein, human; D010088:Oxidoreductases; D007535:Isomerases; C513196:PDIA3 protein, rat; C496702:PDILT protein, human; C513195:Pdia3 protein, mouse; C496703:Pdilt protein, mouse; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human