First isolation and molecular characterization of Ehrlichia canis in Spain.
This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the 16S rRNA gene sequences of other Ehrlichia strains accessible in GenBank. The 16S rRNA gene sequence of the E. canis strain reported here showed a high percentage of similarity with the 16S rRNA gene sequence of E. canis from different geographic areas including Japan, Venezuela and Israel. These data confirm the presence of E. canis in Spain.
Aguirre
Enara
E
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Sainz
Angel
A
Dunner
Susana
S
Amusategui
Inmaculada
I
López
Lissette
L
Rodríguez-Franco
Fernando
F
Luaces
Inés
I
Cortés
Oscar
O
Tesouro
Miguel A
MA
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000188:drug therapy / Q000469:parasitology*; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics* / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000188:drug therapy / Q000469:parasitology* / Q000662:veterinary*; D017809:Fatal Outcome; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007095:Imidocarb / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013030:Spain
D000913:Antibodies, Protozoan; D000981:Antiprotozoal Agents; D016054:DNA, Protozoan; D007095:Imidocarb; C031719:imidocarb dipropionate