Non-invasive method for sampling and extraction of mouse DNA for PCR.
We adapted a non-invasive, fast, reliable and inexpensive procedure for the sampling and extraction of deoxyribonucleic acid (DNA) for genetic testing of mice. The procedure is based on a simple DNA extraction procedure used in the forensic genetic testing of humans. It involves mouth swabbing of the inner cheek using a cotton stick, followed by alkaline lysis of the harvested buccal epithelial cells. This procedure allows for repeated sampling and genetic testing of the individual mouse, and it is faster, simpler and, in our hands, more reliable than the currently used routine procedures for the sampling and extraction of mouse DNA. Current procedures all involve biopsy of a piece of the tail, ear or toe, followed by lengthy procedures to release and isolate the DNA.
Department of Anatomy and Neurobiology, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark. mmeldgaard@health.sdu.dk
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D000818:Animals; D002610:Cheek / Q000502:physiology; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004847:Epithelial Cells / Q000737:chemistry; D051379:Mice / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*
D004247:DNA