A study on biomineralization behavior of N-methylene phosphochitosan scaffolds.
Biomimetic growth of calcium phosphate over natural polymer may be an effective approach to constituting an organic/inorganic composite scaffold for bone tissue engineering. In this work, N-methylene phosphochitosan (NMPCS) was prepared via formaldehyde addition and condensation with phosphoric acid in a step that allowed homogeneous modification without obvious deterioration in chitosan (CS) properties. The NMPCS obtained was characterized by using FT-IR and elemental analysis. The macroporous scaffolds were fabricated through a freeze-drying technique. A comparative study on NMPCS and CS scaffold biomimetic mineralization was carried out in different media, i.e, a simulated body fluid (SBF) or alternative CaCl(2) and Na(2)HPO(4) solutions respectively. Apatite formation within NMPCS and CS scaffolds was identified with FT-IR, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) and X-ray diffractometery (XRD). The results revealed alternate soaking of the scaffolds in CaCl(2) and Na(2)HPO(4) solutions was better than soaking in SBF solution alone in relation to apatite deposition on the scaffold pore walls. Biomineralization provides an approach to improve nature derived materials, e.g., chitosan derivative NMPCS properties e.g., compressive modulus, etc. SEM image of a NMPCS/apatite composite scaffold.
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China.
Yu Ji
YJ
Yin
Xiao Yan
XY
Luo
Jun Feng
JF
Cui
Chang Yong
CY
Wang
Xi Min
XM
Guo
Kang De
KD
Yao
D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001709:Biotechnology / Q000379:methods; D002122:Calcium Chloride / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D006838:Hydrocarbons; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D010766:Phosphorylation; D011108:Polymers; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
D001031:Apatites; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006838:Hydrocarbons; D011108:Polymers; C030011:carbene; D048271:Chitosan; C020243:calcium phosphate; D002122:Calcium Chloride; D008697:Methane