Detection of stanozolol and its metabolites in equine urine by liquid chromatography-electrospray ionization ion trap mass spectrometry.
The equine phase I and phase II metabolism of the synthetic anabolic steroid stanozolol was investigated following its administration by intramuscular injection to a thoroughbred gelding. The major phase I biotransformations were hydroxylation at C16 and one other site, while phase II metabolism in the form of sulfate and beta-glucuronide conjugation was extensive. An analytical procedure was developed for the detection of stanozolol and its metabolites in equine urine using solid phase extraction, acid solvolysis of phase II conjugates and analysis by positive ion electrospray ionization ion trap LC-MS.
Australian Racing Forensic Laboratory, PO Box 528, Kensington, NSW 1465, Australia. amckinney@racingnsw.com.au
Andrew R
AR
McKinney
Craig J
CJ
Suann
Anthony J
AJ
Dunstan
Stephen L
SL
Mulley
Damon D
DD
Ridley
Allen M
AM
Stenhouse
D045930:Anabolic Agents / Q000493:pharmacokinetics / Q000652:urine*; D000818:Animals; D001711:Biotransformation; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013197:Stanozolol / Q000493:pharmacokinetics / Q000652:urine*
D045930:Anabolic Agents; D013197:Stanozolol