Single-particle sizing from light scattering by spectral decomposition.
A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum's peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 microm at a wavelength of 0.633 microm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 microm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others.
Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Institutskaya 3, Novosibirsk 630090 Russia.
Konstantin A
KA
Semyanov
Peter A
PA
Tarasov
Alexey E
AE
Zharinov
Andrei V
AV
Chernyshev
Alfons G
AG
Hoekstra
Valeri P
VP
Maltsev
D000465:Algorithms; D048429:Cell Size; D004912:Erythrocytes / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D047569:Laser Scanning Cytometry / Q000379:methods*; D010316:Particle Size; D012542:Scattering, Radiation