[Water permeability of the OMCD and IMCD cells' basolateral membrane under the conditions of dehydration and dDAVP action].
Water permeability of the basolateral membrane was estimated in isolated fragments of OMCD or IMCD in the Wistar rats. Apical surface of the fragments was blocked with oil injected into the lumen. Apparent water permeability coefficient (Pf) was measured by the rate of epithelium swelling following transition from hypertonic to isotonic medium (600 mOsm to 300 mOsm). Water deprivation caused significant increase in the Pf value in OMCD and IMCD fragments. Desmopressin (10(-8) M) increased water permeability in hydrated rats both in OMCD and IMCD. Mercury chloride decreased the Pf and abolished the effect of desmopressin in reversible manner. Estimation of aquaporins 2, 3, 4 mRNA content in the renal medulla was performed by semi-quantitative RT-PCR. Content of AQP4 and AQP2 mRNA in dehydrated animals was significantly higher than in hydrated ones both in outer medulla and inner medulla. Expression of AQP3 increased during dehydration only in the inner medulla. The findings reveal that water permeability of OMCD and IMCD can be increased by physiological stimuli, e.g. water deprivation. The activation of gene expression of the key elements of vasopressin signal system seems to contribute to this reaction.
Baturina
G S
GS
Isaeva
L E
LE
Khodus
G R
GR
Nesterov
V V
VV
Solenov
E I
EI
Ivanova
L N
LN
D000818:Animals; D020346:Aquaporins / Q000096:biosynthesis* / Q000235:genetics; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002463:Cell Membrane Permeability; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D003681:Dehydration / Q000235:genetics / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005260:Female; D015870:Gene Expression; D007038:Hypotonic Solutions / Q000494:pharmacology; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017669:Mercury Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics; D014867:Water / Q000378:metabolism*
D020346:Aquaporins; D007038:Hypotonic Solutions; D017669:Mercury Compounds; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D014867:Water; D003894:Deamino Arginine Vasopressin; C015728:calomel