Elicitor-induced nitric oxide burst is essential for triggering catharanthine synthesis in Catharanthus roseus suspension cells.
Elicitor prepared from the cell walls of Penicillium citrinum induced multiple responses in Catharanthus roseus suspension cells, including rapid generation of nitric oxide (NO), sequentially followed by enhancement of catharanthine production by C. roseus cells. Elicitor-induced catharanthine biosynthesis was blocked by NO-specific scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and nitric oxide synthase (NOS) inhibitor S,S'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea (PBITU). PBITU also strongly inhibited elicitor-induced NO generation by C. roseus suspension cells. The inhibiting effect of PBITU on elicitor-induced catharanthine production was reversed by external application of NO via the NO-donor sodium nitroprusside. The results strongly suggested that NO, generated by NOS or NOS-like enzymes in C. roseus suspension cells when treated with the fungal elicitor, was essential for triggering catharanthine synthesis.
Xu
Maojun
M
College of Food Sciences and Biotechnology and Environmental Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. maojunxu@163.com
Dong
Jufang
J
D001565:Benzoates; D030008:Catharanthus / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009599:Nitroprusside / Q000378:metabolism; D010407:Penicillium / Q000737:chemistry*; D016897:Respiratory Burst; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014748:Vinca Alkaloids / Q000096:biosynthesis*
D001565:Benzoates; D004791:Enzyme Inhibitors; D007093:Imidazoles; D014748:Vinca Alkaloids; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009599:Nitroprusside; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D013890:Thiourea; C017836:catharanthine