Expression of the tudor-related gene Tdrd5 during development of the male germline in mice.
Homologues of Drosophila germ cell determinant genes such as vasa, nanos and tudor have recently been implicated in development of the male germline in mice. In the present study, the mouse gene encoding Tudor domain containing protein 5 (TDRD5) was isolated from a 12.5-13.5 days post coitum (dpc) male-enriched subtracted cDNA library. Whole-mount in situ hybridization analysis of Tdrd5 expression in the mouse embryonic gonad indicated that this gene is upregulated in the developing testis from 12.5 dpc, with expression levels remaining higher in testis than ovary throughout embryogenesis. Expression of Tdrd5 was absent in testes isolated from We/We embryos, which lack germ cells. In situ hybridization (ISH) on cryosectioned 13.5 dpc testes suggests that expression of Tdrd5, like that of Oct4, is restricted to germ cells. Northern hybridization analysis of expression in adult tissues indicated that Tdrd5 is expressed in the testis only, implying that expression of this gene is restricted to the male germline throughout development to adulthood.
Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia.
James M
JM
Smith
Josephine
J
Bowles
Megan
M
Wilson
Rohan D
RD
Teasdale
Peter
P
Koopman
D000818:Animals; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D013737:Testis / Q000196:embryology* / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D011506:Proteins; D017411:Ribonucleoproteins, Small Nuclear; C488681:Tdrd1 protein, mouse; C498338:Tdrd5 protein, mouse