Measurement of chloride transport in nasal epithelial cells by X-ray microanalysis.
Nasal epithelial cells are a representative and easy to obtain system of epithelial cells for the study of chloride transport. X-ray microanalysis (XRMA) allows the direct and simultaneous measurement of several elements of biological interest, including chloride. Here, a method is presented for the preparation of nasal epithelial cells for X-ray microanalysis and for the determination of chloride transport by this analytical technique.
Dragomir
Anca
A
Department of Medical Cell Biology, University of Uppsala, Box 571, 75123 Uppsala, Sweden.
Andersson
Charlotte
C
Roomans
Godfried M
GM
D002712:Chlorides / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D017136:Ion Transport / Q000502:physiology*; D008854:Microscopy, Electron / Q000379:methods*; D009297:Nasal Mucosa / Q000502:physiology*
D002712:Chlorides