Brassinolides and IAA induce the transcription of four alpha-expansin genes related to development in Cicer arietinum.
Four different cDNAs encoding alpha-expansins have been identified in Cicer arietinum (Ca-EXPA1, Ca-EXPA2, Ca-EXPA3 and Ca-EXPA4). The shared amino acid sequence similarity among the four alpha-expansin proteins ranged from 67 to 89%. All of them display common characteristics such as molecular mass (around 24 kDa), amino acid numbers, and also the presence of a signal peptide. The transcription pattern of chickpea alpha-expansin genes in seedlings and plants suggests a specific role for each of the four alpha-expansins in different phases of development or in different plant organs. High levels of Ca-EXPA2 transcripts coincide with maximum epicotyl and stem growth, indicating an important involvement of this particular alpha-expansin in elongating tissues. Ca-EXPA3 would be related to radicle development, while Ca-EXPA4 seems to be involved in pod development. A considerable increase in the level of all Ca-EXPA transcripts accompanied the indole acetic acid (IAA) plus brassinolide (BR)-induced elongation of excised epicotyl segments. This IAA + BR induction was seen even for the chickpea expansin genes whose transcription was not affected by IAA or BR alone.
Sánchez
Ma Angeles
MA
Departamento de Fisiología Vegetal, Facultad de Biología, Centro Hispano-Luso de Investigaciones Agrarias, Universidad de Salamanca, Pza Doctores de la Reina s/n, Salamanca 37007, Spain.
Mateos
Isabel
I
Labrador
Emilia
E
Dopico
Berta
B
D000595:Amino Acid Sequence; D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D029863:Cicer / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics
D060406:Brassinosteroids; D002777:Cholestanols; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012337:RNA, Ribosomal, 18S; D013260:Steroids, Heterocyclic; C095825:expansin protein, plant; C030737:indoleacetic acid; C023623:brassinolide