Utility of the FLP-FRT recombination system for genetic manipulation of rice.
To develop an FLP-FRT recombination system- (derived from 2 mu plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the beta-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, USA. pradhak@uark.edu
Parthiban
P
Radhakrishnan
Vibha
V
Srivastava
D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D025801:Ubiquitin / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D025801:Ubiquitin; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase