Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay.
The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.
Division of Microbiology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont., Canada M5G 1X8.
D
D
Adachi
G
G
Johnson
R
R
Draker
M
M
Ayers
T
T
Mazzulli
P J
PJ
Talbot
R
R
Tellier
D001483:Base Sequence; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D028941:Coronavirus 229E, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D028962:Coronavirus OC43, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology
D018076:DNA, Complementary; D004279:DNA, Viral; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific