Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province.
OBJECTIVE
To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at the high-incidence area for ESCC in Henan Province, China.


METHODS
Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.


RESULTS
A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin.


CONCLUSIONS
Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC.
An
Ji-Ye
JY
Laboratory for Cancer Research, Third Teaching Hospital, College of Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
Fan
Zong-Min
ZM
Zhuang
Ze-Hao
ZH
Qin
Yan-Ru
YR
Gao
Shan-Shan
SS
Li
Ji-Lin
JL
Wang
Li-Dong
LD
D000368:Aged; D001798:Blood Proteins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000453:epidemiology / Q000601:surgery*; D002681:China / Q000453:epidemiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004938:Esophageal Neoplasms / Q000097:blood* / Q000453:epidemiology / Q000601:surgery*; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins