Syndecan-dependent binding of Drosophila hemocytes to laminin alpha3/5 chain LG4-5 modules: potential role in sessile hemocyte islets formation.
Heparin-column chromatography and elastase-digestion of medium from hemocyte Kc167 gave Drosophila laminin alpha3/5betagamma trimer, alpha3/5LG2-3 and alpha3/5LG4-5 modules with eluting NaCl concentrations of 450, 280 and 450 mM, respectively. Kc167 cells bound dish surface with alpha3/5betagamma trimer or alpha3/5LG4-5, but not with alpha3/5LG2-3 modules. Cell binding was counteracted by treating with heparin or heparan sulfate. RNA interference of syndecan in Kc167 cells impaired the binding, but that of dally or dally-like did not. Green fluorescent protein-expressing hemocytes also bound surface with alpha3/5betagamma trimer or alpha3/5LG4-5 module. Thus, syndecan-dependent binding of hemocytes to laminin may have a potential role in sessile hemocytes islets formation in T2-A8 segments of Drosophila.
Narita
Ryo
R
Department of Bioengineering Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Yamashita
Hironobu
H
Goto
Akira
A
Imai
Hiromi
H
Ichihara
Shigeyuki
S
Mori
Hitoshi
H
Kitagawa
Yasuo
Y
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004330:Drosophila / Q000166:cytology* / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D007814:Larva; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000378:metabolism*; D034622:RNA Interference; D053668:Syndecan-1; D053670:Syndecan-3; D053667:Syndecans; D029867:Xenopus Proteins / Q000378:metabolism
D006493:Heparin; D006497:Heparan Sulfate; D007797:Laminin; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D053668:Syndecan-1; D053670:Syndecan-3; D053667:Syndecans; D029867:Xenopus Proteins; C508244:SDC1 protein, Xenopus; C508316:SDC3 protein, Xenopus; C097043:laminin alpha5; C095567:laminin alpha 3