The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel.
Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.
Sheppard
David N
DN
Department of Physiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. D.N.Sheppard@bristol.ac.uk
Gray
Michael A
MA
Gong
Xiandi
X
Sohma
Yoshiro
Y
Kogan
Ilana
I
Benos
Dale J
DJ
Scott-Ward
Toby S
TS
Chen
Jeng-Haur
JH
Li
Hongyu
H
Cai
Zhiwei
Z
Gupta
Jyoti
J
Li
Canhui
C
Ramjeesingh
Mohabir
M
Berdiev
Bakhrom K
BK
Ismailov
Iskander I
II
Bear
Christine E
CE
Hwang
Tzyh-Chang
TC
Linsdell
Paul
P
Hug
Martin J
MJ
D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008051:Lipid Bilayers; D018408:Patch-Clamp Techniques / Q000379:methods*
C505032:CFTR protein, human; D008051:Lipid Bilayers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator