Expression of Stra13 during mouse endochondral bone development.
We have examined the expression of the basic helix-loop-helix factor Stra13 (DEC1/Sharp2) during endochondral bone development in the mouse. Stra13 expression was examined by in situ hybridization in the tibia from E14.5-E18.5, and at post-natal day 24. At E14.5, expression of Stra13 mRNA was very low, with expression limited to scattered hypertrophic chondrocytes. At E15.5 Stra13 mRNA was present in post-mitotic hypertrophic chondrocytes, co-localizing with collagen X expression. At E16.5-E18.5, Stra13 was expressed in both the proliferating chondrocytes and in the late hypertrophic chondrocytes. At E15.5-E18.5, Stra13 expression was also observed in the primary spongiosa. Stra13 expression was also maintained in the 24-day post-natal tibia, with expression detectable only in the late hypertrophic chondrocytes. Because Stra13 has been shown to be induced by hypoxia, and the growth plate is hypoxic during embryonic development, we compared the expression pattern of Stra13 and the HIF1-alpha target gene VEGF. VEGF is expressed predominantly in the late hypertrophic chondrocytes, with lower expression in the proliferating chondrocytes. Thus, there was a large degree of overlap in the expression patterns of Stra13 and VEGF in chondrocytes during embryonic development.
MacLean
Helen E
HE
Endocrine Unit, WEL501, Massachusetts General Hospital, 50 Blossom St, Boston, MA 02114, USA.
Kronenberg
Henry M
HM
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D001846:Bone Development; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism*; D002356:Cartilage / Q000166:cytology; D019902:Chondrocytes / Q000378:metabolism; D024121:Collagen Type X / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000502:physiology*; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D051792:Basic Helix-Loop-Helix Proteins; D024121:Collagen Type X; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D014157:Transcription Factors; D042461:Vascular Endothelial Growth Factor A; C491539:Bhlhe40 protein, mouse