Preliminary analysis of CDK2 sequence and its nuclear import.
We constructed the plasmids encoding enhanced green fluorescent protein (EGFP)-tagged wild type cyclin-dependent kinase 2(CDK2) (pEGFP-CDK2) and CDK2 deletion mutants (pEGFP-CDK2N and pEGFP-CDK2C, lacking the last C-terminal and the first N-terminal 97 amino acids of CDK2, respectively) and transfected them into HeLa cell line and CHO cell line. After synchronization, green fluorescent signals were detected mainly in nucleus of the cells transfected with pEGFP-CDK2 and predominantly in cytoplasm of the cells transfected with the two mutant CDK2 constructs. Our results suggested that there were no nuclear-import signals in CDK2 and that CDK2 nuclear import might be mediated by association with other proteins through the three-dimensional structure formed by amino acids including those from the N- and C-terminal regions of CDK2.
The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology, China Medical University, Shenyang 110001, China. liuqien@hotmail.com
Qi
Q
Liu
Yang
Y
Luo
Li
L
Jiang
Wei-Qiang
WQ
Zhou
Xiao-Hui
XH
Man
Xue
X
Zhang
D021581:Active Transport, Cell Nucleus; D042846:CDC2-CDC28 Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2; D006367:HeLa Cells; D006801:Humans; D019913:Nuclear Localization Signals; D014162:Transfection
D019913:Nuclear Localization Signals; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2