Site-specific assembly of DNA and appended cargo on arrayed carbon nanotubes.
We describe a strategy that permits discrete regions of arrayed carbon nanotubes (CNTs) to be functionalized simultaneously and specifically with DNA oligonucleotides. The different chemical properties of two regions on single CNTs and orthogonal chemical coupling strategies have been exploited to derivatize CNTs within highly ordered arrays with multiple DNA sequences. Through duplex hybridization, we then targeted different DNA sequences with appended metal nanoparticles to distinct sites on the CNT architecture with precise spatial control. The materials generated from these studies represent the first CNTs with bipartite functionalization. The approach described provides a high level of precision in parallel and directed assembly of DNA sequences and appended cargo and is useful for the preparation of novel hybrid bionanomaterials.
Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.
Bradford J
BJ
Taft
Adam D
AD
Lazareck
Gary D
GD
Withey
Aijun
A
Yin
J M
JM
Xu
Shana O
SO
Kelley
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry
D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D009841:Oligonucleotides; D011721:Pyrenes; D004247:DNA