[Effect of Ginkgo biloba L leaves on oxidation of human low density lipoproteins in vitro].
OBJECTIVE
To study the effects of Ginkgo biloba L leave on oxidation of human low density lipoproteins (LDL) induced by Fe2+ or Cu2+ in vitro.


METHODS
LDL was oxidized by Fe2+ or Cu2+ for 1, 2, 4, 6h, while it was protected by extracts from leaves of Ginkgo biloba L. ( EGB761), ginkgo flavonids(GF) and ginkgo terpenlactones (GT). Lipofusion (LF), malondialdehyde (MDA) and vitamin E (VitE) were measured as the index of the oxidation of LDL by means of Fluorescence spectroscopy methods.


RESULTS
Each extract can inhibit the oxidation of LDL, the inhibition of each extract is increasing during the first 4 hours and subsequently decrease, after 6 hours all the inhibitor have almost lost their ability on antioxidation.


CONCLUSIONS
EGB761, GF and GT are effective antioxidant and the anti-oxidation vary with time.
Huang
Peili
P
Faculty of Public Health, Capital University of Medical Sciences, Beijing 100054, China.
Feng
Gaoqian
G
Zhang
Shuhua
S
Wang
Hui
H
Jin
Youyu
Y
D000975:Antioxidants / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D018515:Plant Leaves / Q000737:chemistry; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007783:Lactones; D008077:Lipoproteins, LDL; D014810:Vitamin E; D008315:Malondialdehyde