Dietary protein concentration affects plasma arteriovenous difference of amino acids across the porcine mammary gland.
The objective of this study was to determine whether the porcine mammary gland responds to increasing dietary CP concentration through changes in AA arteriovenous difference (a-v). Sixteen Landrace x Yorkshire lactating sows were provided ad libitum access to one of four isocaloric diets varying in CP concentration (7.8, 13.0, 18.2, and 23.5 %; as-fed basis). Litters were adjusted to 11 pigs within 48 h of birth. Sows were fitted with catheters in the carotid artery and main mammary vein on d 4. On d 10, 14, 18, and 22 of lactation, arterial and venous blood samples were obtained every 30 min over 6 h. Milk yield was estimated on d 11 and 21 using the D2O dilution technique. Final litter sizes on d 21 were 10.3, 11, 9.5, and 11 piglets for sows fed the 7.8, 13.0, 18.2, and 23.5% CP diets, respectively. Piglet ADG tended (P = 0.088) to increase with increasing dietary CP concentration and were 186, 221, 220, and 202 g for sows fed the 7.8, 13.0, 18.2, and 23.5% CP diet, respectively. Daily total milk yield on d 21 (kg milk/d) tended (P = 0.099) to increase, and average milk yield per nursed piglet (kg of milk-pig(-1)d(-1)) increased (P < 0.05) with increasing CP concentration and were, on a per-piglet basis, 0.95, 1.19, 1.14 and 1.13 kg of milk/d for the 7.8, 13.0, 18.2, and 23.5% CP diets, respectively. As dietary CP increased from 7.8 to 23.5%, isoleucine and leucine a-v increased linearly only (linear, P < 0.01); all other AA a-v increased, reached a maximum in sows fed 18.2% CP, and decreased thereafter in sows fed 23.5% CP (quadratic, from P = 0.10 to P < 0.05). Amino acid uptake by the entire udder and by each gland increased (linear, P < 0.05) with increasing dietary CP. Arteriovenous differences response to increasing day of lactation varied among AA, from no change for histidine, isoleucine, lysine, methionine, tryptophan, and valine, to a linear trend increase for arginine (P = 0.055), leucine (P = 0.064), phenylalanine (P = 0.101), and threonine (P = 0.057). In summary, for the majority of AA, a-v increased with increasing dietary CP concentration from 7.8 to 18.2%, but decreased when CP concentration exceeded 18.2%. In contrast, mammary AA uptake, piglet ADG and milk yield per pig increased linearly with increasing dietary CP, suggesting a coordinated regulation between AA delivery and transport to meet the demand for milk yield.
Guan
X
X
Department of Animal Science, Michigan State University, East Lansing 48824, USA.
Pettigrew
J E
JE
Ku
P K
PK
Ames
N K
NK
Bequette
B J
BJ
Trottier
N L
NL
D000596:Amino Acids / Q000097:blood* / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007774:Lactation / Q000097:blood* / Q000378:metabolism; D008098:Litter Size; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000502:physiology; D008892:Milk / Q000378:metabolism; D009751:Nutritional Requirements; D011897:Random Allocation; D012039:Regional Blood Flow; D013552:Swine / Q000097:blood / Q000378:metabolism / Q000502:physiology*
D000596:Amino Acids; D004044:Dietary Proteins