Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms.
The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei.
Zootechnical Sciences and Food Inspection Department, School of Agriculture, University of Naples, Federico II, Via Università 133, Portici, 80055 Naples, Italy. capparel@unina.it
R
R
Capparelli
M
M
Viscardi
M G
MG
Amoroso
G
G
Blaiotta
M
M
Bianco
D002957:Citrus / Q000145:classification* / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D005838:Genotype; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D021161:Self-Sustained Sequence Replication / Q000379:methods*
D018744:DNA, Plant; D005819:Genetic Markers