Production of hemo- and immunoregulatory cytokines by erythroblast antigen+ and glycophorin A+ cells from human bone marrow.
BACKGROUND
Erythroid nuclear cells (ENC) of the bone marrow (BM) have not previously been considered as important producers of wide spectrum of haemo- and immunoregulatory cytokines. The aim of the current work was to confirm the production of the main hemo- and immunoregulatory cytokines in human ENC from BM.


RESULTS
We used native human BM ENC in our experiments. We for the first time have shown, that the unstimulated erythroblasts (Gl A+ or AG-EB+) produced a wide spectrum of immunoregulatory cytokines. Human BM ENC produce cytokines such as interleukin (IL)-1beta, IL-2, IL-4, IL-6, interferon (IFN)-gamma, transforming growth factor (TGF)-beta1, tumor necrosis factor (TNF)-alpha and IL-10. They can be sub-divided into glycophorin A positive (Gl A+) and erythroblast antigen positive (AG-EB+) cells. To study potential differences in cytokine expression between these subsets, ENC were isolated and purified using specific antibodies to Gl A and AG-EB and the separated cells were cultivated for 24 hours. The cytokine contents of the supernatant were measured by electrochemiluminescence immunoassay. Quantitative differences in TGF-beta1 and TNF-alpha production were found between Gl A+ and AG-EB+ BM ENC. Furthermore, in vitro addition of erythropoietin (EPO) reduced IFN-gamma and IL-2 production specifically by the AG-EB+ ENC. Thus, Gl A+ and AG-EB+ ENC produce IL-1beta, IL-2, IL-4, IL-6, IFN-gamma, TGF-beta1 and TNF-alpha. Gl A+ ENC also produce IL-10.


CONCLUSIONS
Cytokine production by erythroid nuclear cells suggests that these cells might be involved in regulating the proliferation and differentiation of hematopoietic and immunocompetent cells in human BM.
Laboratory of the Regulation of Immunopoiesis, Institute of Clinical Immunology SB RAMS, Yadrintsevskaya 14, Novosibirsk, 630099, Russia. ici@online.nsk.su
Sergey V
SV
Sennikov
Tatyana V
TV
Injelevskaya
Sergey V
SV
Krysov
Alexandr N
AN
Silkov
Igor B
IB
Kovinev
Natalya J
NJ
Dyachkova
Anton N
AN
Zenkov
Mary I
MI
Loseva
Vladimir A
VA
Kozlov
D000941:Antigens / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology; D001854:Bone Marrow Cells / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology; D004900:Erythroblasts / Q000276:immunology*; D006021:Glycophorins / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000502:physiology; D007375:Interleukin-1 / Q000096:biosynthesis / Q000502:physiology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000502:physiology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000502:physiology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000502:physiology; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000941:Antigens; D016207:Cytokines; D006021:Glycophorins; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma