[Some mechanisms of adenosine protective effect in the "calcium paradox"].
A possibility of preventing the "calcium paradox" with the aid of adenosine was studied as well as some mechanisms of adenosine effect upon the heart in case of the "calcium paradox". Adenosine was found to suppress release of amino acids from the heart in perfusion with calcium-free medium, to efficiently prevent disorders in the energy-dependent functions of mitochondrion and myoglobin release from the heart in reperfusion with Ca2+ -containing solution. Adenosine was also found to increase 2-10-fold lactate release from the heart. Adenosine seems to be able to activate glycolysis. Iodine acetate was shown to completely suppress the adenosine ability to decrease amino acid release from the heart perfused with calcium-free medium. Under conditions of iodine acetate blocking of glycolysis was found to possess no protective properties against cytolysis in the "calcium paradox". The heart mitochondria isolated in the end of the experiment revealed low values of free or phosphorylating respiration and complete dissociation of oxidation. Also a protective effect of adenosine in inhibition of Na+, K+ -ATPhase with Strophantinum, was studied.
V V
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Alabovskiĭ
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Khamburov
A A
AA
Vinokurov
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D006019:Glycolysis / Q000187:drug effects; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009211:Myoglobin / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000502:physiology; D013328:Strophanthins / Q000494:pharmacology
D009211:Myoglobin; D020011:Protective Agents; D013328:Strophanthins; D000254:Sodium-Potassium-Exchanging ATPase; D000241:Adenosine; D002118:Calcium