Amperometric hydrogen peroxide biosensor based on horseradish peroxidase-labeled nano-Au colloids immobilized on poly(2,6-pyridinedicarboxylic acid) layer by cysteamine.
A sensitive hydrogen peroxidase (H2O2) amperometric sensor based on horseradish peroxidase (HRP)-labeled nano-Au colloids has been proposed. Nano-Au colloids were immobilized by the thiol group of cysteamine, which was associated with the carboxyl groups of poly(2,6-pyridinedicarboxylic acid) (PPDA). With the aid of the hydroquinone, the sensor displayed excellent electrocatalytical response to the reduction of H2O2. Compared with the non-Au-colloid modified electrode, i.e., PPDA/HRP, the Au-colloid modified electrode exhibited better performance characteristics, including stability, reproducibility, sensitivity and accuracy. The biosensor shows a linear response to H2O2 in the range of 3.0 x 10(-7) - 2 x 10(-3) M. The detection limit was 1.0 x 10(-7) M.
College of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, P R. China. lichx33@etang.com
Chun-Xiang
CX
Li
Ke-Qing
KQ
Deng
Guo-Li
GL
Shen
Ru-Qin
RQ
Yu
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003543:Cysteamine / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D049329:Nanostructures; D010848:Picolinic Acids; D011108:Polymers; D011725:Pyridines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D017979:Gold Colloid; D010848:Picolinic Acids; D011108:Polymers; D011725:Pyridines; D003543:Cysteamine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C004860:dipicolinic acid