[Evaluation of bacterial flora in contaminated soil as a countermeasure against H2S gas production].
Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, spore-forming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium.
Taniguchi
Hatsumi
H
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Fukuda
Kazumasa
K
Yan
Wan
W
Hinoue
Mitsuo
M
Yamauchi
Kazuki
K
Ichihara
Tsuyoshi
T
Mizuno
Kouhei
K
Ishimatsu
Sumiyo
S
Sera
Nobuyuki
N
Hamasaki
Mitsuhiro
M
Takahashi
Kouji
K
Horikawa
Kazumi
K
D000397:Air Pollution / Q000517:prevention & control*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D003199:Computer Systems; D005740:Gases; D006862:Hydrogen Sulfide / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D014866:Waste Products
D005740:Gases; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014866:Waste Products; D006862:Hydrogen Sulfide