Subcellular domain-restricted GABAergic innervation in primary visual cortex in the absence of sensory and thalamic inputs.
Distinct classes of GABAergic synapses target restricted subcellular domains, thereby differentially regulating the input, integration and output of principal neurons, but the underlying mechanism for such synapse segregation is unclear. Here we show that the distributions of two major classes of GABAergic synapses along the perisomatic and dendritic domains of pyramidal neurons were indistinguishable between primary visual cortex in vivo and cortical organotypic cultures. Therefore, subcellular synapse targeting is independent of thalamic input and probably involves molecular labels and experience-independent forms of activity.
Di Cristo
Graziella
G
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Wu
Caizhi
C
Chattopadhyaya
Bidisha
B
Ango
Fabrice
F
Knott
Graham
G
Welker
Egbert
E
Svoboda
Karel
K
Huang
Z Josh
ZJ
D000344:Afferent Pathways / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D048429:Cell Size; D022081:Cell Surface Extensions / Q000502:physiology*; D016009:Chi-Square Distribution; D007395:Interneurons / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008959:Models, Neurological; D009924:Organ Culture Techniques; D010320:Parvalbumins / Q000235:genetics; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology*; D013004:Somatostatin / Q000235:genetics; D013569:Synapses / Q000145:classification / Q000502:physiology*; D013788:Thalamus / Q000502:physiology*; D014793:Visual Cortex / Q000166:cytology* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010320:Parvalbumins; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid