Impaired absorption of marked oligopeptide Glycine-I Tyrosine-Glycine after successful autologous-allotopic ileal mucosa transplantation in beagles.
OBJECTIVE
After establishing a method for ileal mucosa transplantation in an animal model, the authors investigated the absorptive capacity for oligopeptides of the transplanted mucosa.


METHODS
In 14 beagle dogs the authors transplanted ileal mucosa in a vascularized demucosed segment of the transverse colon. The colonic wall-ileal mucosa complex then was integrated in the ileal continuity. Six animals were lost owing to operative complications. Absorptive capacity for oligopeptides was measured in the remaining 8 animals with the iodine 131 (131I)-marked tripeptide glycine-tyrosine-glycine before and 4 weeks after transplantation. The results were compared and analyzed with the Student's t test for matched pairs. Blood concentrations of the marked tripeptide with P value less than .05 were considered as a significant reduction in the absorptive capacity of the transplanted ileal mucosa. After fixation with glutaraldehyd graft, uptake of the colonic wall-ileal mucosa complex was evaluated histologically in 8 animals.


RESULTS
In all 8 animals, a 100% graft uptake was verified in all sections. Fifteen minutes after application of 15 MBc Glycine-131I-Tyrosine-Glycine there was no significant difference in the absorption between normal and transplanted ileal mucosa. After 30 minutes, the absorption of the transplanted ileal mucosa showed a tendency (P < .1) for an impaired uptake of the marked tripeptide. However, 60 minutes after application the difference in the absorptive capacity of the transplanted ileal mucosa was significant (P < .05).


CONCLUSIONS
Autologous allotopic ileal mucosa transplantation is feasible; however, an impaired absorption of oligopeptides of the transplanted mucosa 4 weeks after transplantation could be observed.
Department of Pediatric Surgery, University of Heidelberg, Heidelberg, Germany.
H A
HA
Beiler
J
J
Steinorth
A
A
Witt
W
W
Mier
A
A
Mohammed
K L
KL
Waag
Z
Z
Zachariou
D000818:Animals; D004285:Dogs; D007082:Ileum / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D009842:Oligopeptides / Q000493:pharmacokinetics*; D012778:Short Bowel Syndrome / Q000378:metabolism*; D014182:Transplantation, Autologous / Q000502:physiology
D009842:Oligopeptides; C503208:glycyl-iodine (131I)-tyrosyl-glycine