Microarrays and beyond: what potential do current and future genomics tools have for breeders?
One of the most exciting tools from genomics is the ability to obtain a whole-genome snapshot of gene expression. This is typically called a microarray analysis, because probes for the genes of interest, which can run into the thousands, are spotted in a very small array on a glass slide or some other substrate. The resulting array is often called a gene chip, or simply a chip, in the case of short oligo arrays, or slides in the case of cDNA or long oligo arrays. Microarrays offer the awesome potential of simultaneously examining the level of expression, where expression is intended to measure the standing amount of mRNA, for all of the genes in a genome. Given this potential, it is not surprising that microarrays have attracted a great deal of attention from animal geneticists and breeders. The purpose of this review is to provide a brief, yet critical, overview of some of the potential uses of such whole-genome expression studies in applied animal breeding and to speculate about what additional forthcoming tools might be of use.
Walsh
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Department of Ecology and Evolutionary Biology, University of Arizona, Tucson 85721, USA. jbwalsh@u.arizona.edu <jbwalsh@u.arizona.edu>
Henderson
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D
D000818:Animals; D001709:Biotechnology / Q000379:methods / Q000639:trends*; D001947:Breeding / Q000379:methods*; D015870:Gene Expression; D023281:Genomics / Q000379:methods / Q000639:trends*; D046228:Microarray Analysis / Q000379:methods / Q000639:trends*; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger