Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan.
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.
Anada
Takahisa
T
Department of Chemical Process and Environments, The University of Kitakyushu, 1-1 Hibikino, Wakamatu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
Matsunaga
Hideshi
H
Karinaga
Ryouji
R
Koumoto
Kazuya
K
Mizu
Masami
M
Nakano
Koji
K
Shinkai
Seiji
S
Sakurai
Kazuo
K
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004247:DNA / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D008969:Molecular Sequence Data; D011061:Poly A / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012566:Sizofiran / Q000737:chemistry*; D013997:Time Factors
D011134:Polysaccharides; D011061:Poly A; C015465:poly(dA); D012566:Sizofiran; D004247:DNA