1274 full-open reading frames of transcripts expressed in the developing mouse nervous system.
As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to approximately 20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs > or =5 kb, and 54% of those > or =4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins.
Bonaldo
Maria F
MF
Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA.
Bair
Thomas B
TB
Scheetz
Todd E
TE
Snir
Einat
E
Akabogu
Ike
I
Bair
Jennifer L
JL
Berger
Brian
B
Crouch
Keith
K
Davis
Aja
A
Eyestone
Mari E
ME
Keppel
Catherine
C
Kucaba
Tamara A
TA
Lebeck
Mark
M
Lin
Jenny L
JL
de Melo
Anna I R
AI
Rehmann
Joshua
J
Reiter
Rebecca S
RS
Schaefer
Kelly
K
Smith
Christina
C
Tack
Dylan
D
Trout
Kurtis
K
Sheffield
Val C
VC
Lin
Jim J-C
JJ
Casavant
Thomas L
TL
Soares
Marcelo B
MB
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism*; D018076:DNA, Complementary; D005123:Eye / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015723:Gene Library; D016678:Genome; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger