Chromatic immunoassay based on polydiacetylene vesicles.
A new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 10,12-tricosadiynoic acid (TCDA) and dimyristoylphosphatidycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay.
Su
Yan-Lei
YL
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
Li
Jin-Ru
JR
Jiang
Long
L
D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry; D003124:Colorimetry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes; D012680:Sensitivity and Specificity; D013499:Surface Properties
C434762:10,12-tricosadiynoic acid; D000906:Antibodies; D005231:Fatty Acids, Unsaturated; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D000114:Acetylene; D004134:Dimyristoylphosphatidylcholine