Control of a multisubunit DNA motor by a thermoresponsive polymer switch.
The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction-modification (R-M) enzymes enables temperature-controlled "switching" of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil-globule phase transitions of the polymer as shown in light scattering and gel retardation assays.
School of Pharmacy and Biomedical Sciences, Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth PO1 2DT, United Kingdom.
Sivanand S
SS
Pennadam
Matthieu D
MD
Lavigne
Christina F
CF
Dutta
Keith
K
Firman
Darren
D
Mernagh
Dariusz C
DC
Górecki
Cameron
C
Alexander
D000180:Acrylic Resins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D021122:Protein Subunits
D000180:Acrylic Resins; D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D021122:Protein Subunits; C052970:poly-N-isopropylacrylamide; D004247:DNA; C109829:HsdM protein, Bacteria; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific