Equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the EcPV-1 L1 major capsid protein.
Equus caballus papillomavirus type 1 (EcPV-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. The complete EcPV-1 nucleotide sequence and genomic organization were determined. Phylogenetic analysis showed that EcPV-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. To produce EcPV-1 virus-like particles (VLPs), the EcPV-1 L1 major capsid protein was expressed in insect cells using a recombinant baculovirus vector. The self-assembled EcPV-1 VLPs were morphologically indistinguishable from wild type papillomavirus virions. Monoclonal antibodies were developed against intact and denatured EcPV-1 VLPs. When tested by ELISA, all monoclonal antibodies produced against intact (#18) and some against denatured EcPV-1 VLPs (#16) reacted with intact EcPV-1 VLPs only, demonstrating that the VLPs carry type-specific conformational as well as linear epitopes on their surface. Recombinant EcPV-1 VLPs offer the potential of a noninfectious vaccine to prevent and eradicate equine cutaneous papillomatosis.
Laboratory of Vaccinology, John Graham Brown Cancer Center, University of Louisville Health Science Center, Louisville, KY, USA.
Shin-Je
SJ
Ghim
Annabel
A
Rector
Hajo
H
Delius
John P
JP
Sundberg
A Bennett
AB
Jenson
Marc
M
Van Ranst
D020816:Amino Acid Motifs; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D016367:Baculoviridae / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D016679:Genome, Viral; D006736:Horses; D015151:Immunoblotting; D007313:Insecta; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000737:chemistry; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics*; D010802:Phylogeny; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014771:Virion
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; C094776:L1 protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; D004247:DNA