Detection of catheter-related bloodstream infections by the differential-time-to-positivity method and gram stain-acridine orange leukocyte cytospin test in neutropenic patients after hematopoietic stem cell transplantation.
For febrile neutropenic patients who received hematopoietic stem cell transplantation, the Gram stain-acridine orange leukocyte cytospin (AOLC) test and the differential-time-to-positivity method (DTP) were performed. As a diagnostic tool for catheter-related bloodstream infections in these patients, the Gram stain-AOLC test has a lower sensitivity than does the DTP method but acceptable positive and negative predictive values.
Division of Infectious Diseases, Department of Internal Medicine, Medical University of Graz, Graz, Austria. robert.krause@meduni-graz.at
R
R
Krause
H W
HW
Auner
G
G
Gorkiewicz
A
A
Wölfler
F
F
Daxboeck
W
W
Linkesch
G J
GJ
Krejs
C
C
Wenisch
E C
EC
Reisinger
D000165:Acridine Orange; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002408:Catheters, Indwelling / Q000009:adverse effects*; D004866:Equipment Contamination; D005456:Fluorescent Dyes; D005840:Gentian Violet; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007962:Leukocytes; D009503:Neutropenia / Q000628:therapy*; D010619:Phenazines; D013194:Staining and Labeling; D013997:Time Factors
D005456:Fluorescent Dyes; C035863:Gram's stain; D010619:Phenazines; D000165:Acridine Orange; D005840:Gentian Violet