Molecular structure and organization of crustacean hyperglycemic hormone genes of Penaeus monodon.
The Crustacean hyperglycemic hormone (CHH) has been shown to exist as multiple molecular forms in several crustacean species. In Penaeus monodon, a gene encoding CHH (so-called Pem-CHH1) was recently described. In this study, the molecular structures of two other CHH genes (Pem-CHH2 and Pem-CHH3) are reported. Both the Pem-CHH2 and Pem-CHH3 genes contain three exons that are separated by two introns that are similar to the structure of other genes in the same family. An analysis of the upstream nucleotide sequences of each Pem-CHH gene has identified the putative promoter element (TATA box) and putative binding sites for several transcription factors. The binding sites for CREB, Pit-1, and AP-1 were found upstream of all three Pem-CHH genes. A Southern blot analysis showed that at least one copy of each Pem-CHH gene was located within the same 10 kb genomic DNA fragment. These results suggest that the CHH genes are arranged in a cluster in the genome of P. monodon, and that their expression may be modulated by similar mechanisms.
Wiwegweaw
Amporn
A
Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.
Udomkit
Apinunt
A
Panyim
Sakol
S
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D018388:Codon, Terminator; D005091:Exons; D005809:Genes, Regulator; D007438:Introns; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics*; D033561:Penaeidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D060829:Arthropod Proteins; D018388:Codon, Terminator; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C015947:hyperglycemic hormone, crustacean