Comparative analysis of cyanobacterial communities from polluted reservoirs in Korea.
Cyanobacteria are the dominant phototrophic bacteria in water environments. Here, the diversity of cyanobacteria in seven Korean reservoir waters where different levels of algal blooms were observed during the summer of 2002, was examined by T-RFLP analysis. The number of T-RF bands in the HaeIII T-RFLP profiles analyzed from those water samples ranged from 20 to 44. Of these, cyanobacteria accounted for 6.1 to 27.2% of the total bacteria. The water samples could be clustered into 2 groups according to the Dice coefficient of the T-RF profiles. The eutrophic Dunpo and oligotrophic Chungju reservoirs were selected, and several representative clones from both reservoir waters analyzed for the nucleotide sequences of their 16S rDNA. The major clones were found to belong to the Microcystis and Anabaena species in the waters from the Dunpo and Chungju reservoirs, respectively, which was in agreement with the T-RFLP result. That is, the Microcystis and Anabaena species were dominant in the eutrophic and polluted Dunpo and oligotrophic Chungju reservoir waters, respectively. These results indicated that there is a correlation between prevalence of cyanobacterial species and levels of pollution in reservoir waters.
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Korea.
Jin-Book
JB
Kim
Mi-Sook
MS
Moon
Dong-Hun
DH
Lee
Sung-Taik
ST
Lee
Marco
M
Bazzicalupo
Chi-Kyung
CK
Kim
D000818:Animals; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D005068:Eutrophication; D005618:Fresh Water / Q000382:microbiology*; D007723:Korea; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014876:Water Pollution
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S