Reverse transcription-booster PCR for detection of noroviruses in shellfish.
The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.
Laboratorio Alimenti, Istituto Superior di Sanità, Rome, Italy. dario.demedici@iss.it.
Dario
D
De Medici
Luciana
L
Croci
Elisabetta
E
Suffredini
Laura
L
Toti
D000818:Animals; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000635:transmission / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005243:Feces / Q000821:virology; D005516:Food Microbiology; D005759:Gastroenteritis / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000821:virology*
D004279:DNA, Viral; D012367:RNA, Viral