Migration and maturation of human dendritic cells infected with Toxoplasma gondii depend on parasite strain type.
Migration and maturation of human dendritic cells derived from CD34+ progenitor cells (DC) infected by Toxoplasma gondii were studied in an in vitro model. We demonstrated that infection with virulent type I strains RH and ENT or type II low virulent strains PRU and CAL induced DC migration towards MIP-3beta. However, type II strains induced a higher percentage of migrating cells than that induced by type I strains or positive controls (chemical allergen or lipopolysaccharides). Type II strains produced soluble factors responsible of the high migration whereas heat killed tachyzoites did not induced a migration higher than positive controls. We also demonstrated that infection by virulent strains and not by type II stains or heat killed tachyzoites triggers DC maturation. A soluble factor released by type II strains was responsible of the absence of DC maturation. Taken together, these results demonstrated that the interference of T. gondii in the behaviour of DC functions is related to the strain types and can be supported by secretion of soluble factors by the parasite.
Diana
Julien
J
EA3732, Université Claude Bernard LYON I, Pavillon R, Hôpital Edouard Herriot, 5 place d'Arsonval, 69437 Lyon 03, France.
Persat
Florence
F
Staquet
Marie-Jeanne
MJ
Assossou
Olga
O
Ferrandiz
Josette
J
Gariazzo
Marie-Jo
MJ
Peyron
François
F
Picot
Stéphane
S
Schmitt
Daniel
D
Vincent
Claude
C
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D051940:B7-2 Antigen; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D054415:Chemokine CCL19; D019742:Chemokines, CC / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology / Q000469:parasitology* / Q000502:physiology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000032:analysis; D009000:Monocytes; D010641:Phenotype; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000032:analysis; D014122:Toxoplasma / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence; D000097802:CD83 Antigen
D015703:Antigens, CD; D018952:Antigens, CD34; D051940:B7-2 Antigen; D054415:Chemokine CCL19; D019742:Chemokines, CC; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D000097802:CD83 Antigen; C515460:CCL19 protein, human; C106311:CCR6 protein, human; C515370:CCR7 protein, human; C497964:CD86 protein, human