Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection.
This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separation and efficiency. The method showed good linearity and precision. Recovery was between 85 and 102% and detection limits were 10, 15 and 20 ng/ml for MDA, MDMA and MDEA, respectively. No interfering substances were detected with fluorescence detection.
Instrumental Analysis Laboratory, Criminalistic Institute, College of Pharmaceutical Sciences, Toxicology, University of São Paulo, Av. Professor Lineu Prestes, 580 B13B-Toxicology, CEP 05508-900 São Paulo, SP, Brazil. jlcefoa@yahoo.com
José Luiz
JL
da Costa
Alice Aparecida
AA
da Matta Chasin
D015104:3,4-Methylenedioxyamphetamine / Q000031:analogs & derivatives* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005554:Forensic Medicine; D006801:Humans; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine; C051800:3,4-methylenedioxyethamphetamine