Imaging gene expression in single living cells.
Technical advances in the field of live-cell imaging have introduced the cell biologist to a new, dynamic, subcellular world. The static world of molecules in fixed cells has now been extended to the time dimension. This allows the visualization and quantification of gene expression and intracellular trafficking events of the studied molecules and the associated enzymatic processes in individual cells, in real time.
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Yaron
Y
Shav-Tal
Robert H
RH
Singer
Xavier
X
Darzacq
D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D003952:Diagnostic Imaging; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D015003:Yeasts / Q000166:cytology / Q000378:metabolism
D004268:DNA-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors