Monomeric C18 chromatographic method for the liquid chromatographic determination of lipophilic antioxidants in plants.
Reversed-phase liquid chromatography was used to determine lipophilic antioxidants in plants using two monomeric C18 columns operated at 30 degrees C and 4 degrees C, with a column-switching technique and acetonitrile-methanol gradient elution. The chromatograms were extracted at different wavelengths using a UV diode array detector (DAD). A wide range of plant antioxidants, including nine carotenoids (neoxanthin, violaxanthin, lutein, zeaxanthin, beta-cryptoxanthin, lycopene, canthaxanthin, alpha-carotene and beta-carotene) together with all-trans-retinol, capsaicin, dihydrocapsaicin, chlorophyll a, and chlorophyll b can be separated within 50 min. Fluorometric detection was applied to quantify trace amounts of six vitamin E analogues (alpha-, delta- and gamma-tocopherols and tocotrienols). The detection limits were 0.2-0.4 microg/g for various xanthophylls and 0.04-0.10 microg/g for vitamin E analogues.
Department of Community, Occupational and Family Medicine, Faculty of Medicine, National University of Singapore, Singapore 117597, Singapore.
Bee Lan
BL
Lee
Jin
J
Su
Choon Nam
CN
Ong
D000975:Antioxidants / Q000032:analysis*; D002211:Capsaicin / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010944:Plants / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000032:analysis; D014810:Vitamin E / Q000032:analysis; D024341:Xanthophylls / Q000032:analysis
D000975:Antioxidants; D024341:Xanthophylls; D014801:Vitamin A; D014810:Vitamin E; D002734:Chlorophyll; D002338:Carotenoids; D002211:Capsaicin