The Canine Parvovirus wild-type strains infections in dogs--epidemiological and diagnostic aspects.
Biological material was taken from dogs with diarrhea. Faecal samples were taken from live animals white intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. In total, 18 specimens were investigated from dogs housed alone or in large groups. The samples were examined for presence of viral infections and concurrent bacterial and parasitic infestations. To test for the presence of the viral infection, latex (On Site Biotech, Sweden) and direct immunofluorescence tests were performed. At the same time to the presence of CPV infection, was conducted by the PCR method with primers complementary to a conservative region of VP1/VP2. In order to identify the bacterial strain, the material was inoculated onto appropriate media and identified with API tests, whilst parasitological examinations were performed with Fulleborn's method. CPV infection was accompanied by CCV and CAV infections, as well as bacterial ones, caused mostly by Escherichia coli.
Department of Epizootiology and Veterinary Administration with Clinic, Faculty of Veterinary Medicine, Agricultural University, Pl Grunwaldzki 45, 50-366 Wrocław, Poland.
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D000818:Animals; D036022:Capsid Proteins / Q000032:analysis; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology / Q000821:virology*; D004285:Dogs; D004926:Escherichia coli / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology / Q000469:parasitology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D007422:Intestines / Q000382:microbiology / Q000469:parasitology; D007841:Latex Fixation Tests / Q000662:veterinary; D008297:Male; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary
D036022:Capsid Proteins; D017931:DNA Primers; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19