Labeling of major plant lipids and jasmonic acid using [1-(14C)] lauric acid.
A medium chain length fatty acid, [1-(14C)] lauric acid (12:0) was administered to the detached leaves of Artemisia and was incorporated into major lipids, including phospholipids and galactolipids. [1-(14C)]12:0 was elongated and desaturated into linolenic acid (18:3). In detached leaves of both Artemisia and Arabidopsis thaliana ecotype Columbia, radioactivity from [14C]18:3 was incorporated into jasmonic acid (JA) and methyl jasmonate (MJ). Higher amounts of [14C]JA were measured in Artemisia than Arabidopsis leaves. In Artemisia, [14C]JA was actively metabolized into [14C]MJ. Extracts prepared from the leaves of Artemisia, exhibited higher in vitro JA methyltransferase activity than those from Arabidopsis.
Department of Agronomy, University of Kentucky, 403 Plant Science Building, 1405 Veterans Drive, Lexington, KY 40546-0312, USA.
Meshack M
MM
Afitlhile
Hirotada
H
Fukushige
David
D
Hildebrand
D017360:Arabidopsis / Q000378:metabolism*; D019306:Artemisia / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003517:Cyclopentanes / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008738:Methyl Ethers / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D054883:Oxylipins; D018515:Plant Leaves / Q000378:metabolism
D002250:Carbon Radioisotopes; D003517:Cyclopentanes; D007850:Lauric Acids; D008055:Lipids; D008738:Methyl Ethers; D054883:Oxylipins; C030358:lauric acid; C011006:jasmonic acid