A standardized endogenous megakaryocytic erythroid colony assay for the diagnosis of essential thrombocythemia.
OBJECTIVE
The reliability of assays of endogenous megakaryocytic colony (EMC) and endogenous erythroid colony (EEC) formation for the diagnosis of thrombocytoses remains controversial. We tested the suitability of a recently developed collagen-based assay of EMC formation for the diagnosis of essential thrombocythemia (ET).


METHODS
This was a multicenter (8 laboratories) study including 121 patients: 82 with ET and 39 with reactive thrombocytoses (RT). EMC and EEC were assessed in each laboratory in serum-free, cytokine-free, standardized collagen gel assays; bone marrow (BM) and peripheral blood (PB) were tested in parallel.


RESULTS
In PB cultures, only EEC were specific for ET. In BM cultures, both EMC and EEC were specific for ET and present in assays of 77.8% (EMC) and 33.3% (EEC) of ET patients. Altogether, 80.2% of ET patients had BM EMC and/or EEC, whereas none of the patients with RT did.


CONCLUSIONS
When performed with BM progenitors for the diagnosis of thrombocytoses, positivity of the standardized EMC/EEC assay in collagen is specific (100%) and detects 80% of ET.
Dobo
Irène
I
Laboratoire d' Hématologie of the Centres Hospitaliers Universitaires d'Angers, France.
Boiret
Nathalie
N
Lippert
Eric
E
Girodon
François
F
Mossuz
Pascal
P
Donnard
Magali
M
Campos
Lydia
L
Pineau
Danielle
D
Bascans
Elisabeth
E
Praloran
Vincent
V
Hermouet
Sylvie
S
D001853:Bone Marrow / Q000473:pathology; D003094:Collagen; D003114:Colony-Forming Units Assay; D003470:Culture Media; D016895:Culture Media, Serum-Free; D015672:Erythroid Precursor Cells / Q000473:pathology*; D006801:Humans; D008533:Megakaryocytes / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013920:Thrombocythemia, Essential / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D013922:Thrombocytosis / Q000097:blood / Q000473:pathology
D003470:Culture Media; D016895:Culture Media, Serum-Free; D003094:Collagen