Grb7 expression and cellular migration in chronic lymphocytic leukemia: a comparative study of early and advanced stage disease.
Grb7, a noncatalytic intracellular adaptor protein involved in cell migration, is overexpressed in certain invasive and metastatic solid tumors. We found a highly significant difference in the level of expression of Grb7 between chronic lymphocytic leukemia (CLL) cells obtained from stage I and stage IV patients (P<0.001). Using semiquantitative RT-PCR, we detected high levels of Grb7 in 88% of stage IV patients vs only 18% in stage I patients. A corresponding increase was found in the in vitro migration of stage IV CLL cells in comparison to stage I cells. The statistically significant difference in the expression of Grb7 between stage IV and stage I patients was preserved even when tested specifically in the ZAP70-positive group (P<0.01). These findings show that Grb7 levels reflect the severity of the disease, and may be used, in conjunction with ZAP70, to predict disease progression.
Hematology Institute, Kaplan Medical Center, Rehovot, Israel. haran@clalit.org.il
M
M
Haran
S
S
Chebatco
L
L
Flaishon
F
F
Lantner
N
N
Harpaz
L
L
Valinsky
A
A
Berrebi
I
I
Shachar
D016022:Case-Control Studies; D002465:Cell Movement; D002478:Cells, Cultured; D004194:Disease; D018450:Disease Progression; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D051382:GRB7 Adaptor Protein; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D009367:Neoplasm Staging; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051746:ZAP-70 Protein-Tyrosine Kinase
C495955:GRB7 protein, human; D011506:Proteins; D051382:GRB7 Adaptor Protein; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human