Translin associated protein X is essential for cellular proliferation.
DNA vectors that express short hairpin RNAs (shRNAs) provide a new tool for reverse genetic analysis for selective long-term reduction of gene expression in mammalian cells. Using shRNA constructs with a cytomegalovirus promoter and an actin intron between the hairpins for stabilization, we reduce expression of an exogenously expressed gene, GFP and the endogenous protein, Translin-associated factor X (TRAX), in stably transfected Hela cell lines. The reduction of TRAX in Hela cells causes reduced cell proliferation. This decrease is specific as there is no equivalent reduction of the TRAX interacting protein, Testis brain RNA-binding protein, or any significant increase in a number of interferon-related target genes.
Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6142, USA.
Shicheng
S
Yang
Norman B
NB
Hecht
D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D005434:Flow Cytometry; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012342:RNA, Small Nuclear / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002352:Carrier Proteins; D009687:Nuclear Proteins; D012342:RNA, Small Nuclear; C104071:TSNAXIP1 protein, human