Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide ligation assay on DNA chips.
A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing.
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Jiao-Yu
JY
Deng
Xian-En
XE
Zhang
Hong-Bing
HB
Lu
Qiang
Q
Liu
Zhi-Ping
ZP
Zhang
Ya-Feng
YF
Zhou
Wei-Hong
WH
Xie
Zhi-Jun
ZJ
Fu
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D011088:DNA Ligases / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D017354:Point Mutation; D012293:Rifampin / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D009841:Oligonucleotides; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D011088:DNA Ligases; D012293:Rifampin