Estrogen receptor alpha and beta expression in uterine leiomyomas from premenopausal women.
OBJECTIVE
To measure messenger RNA levels of estrogen receptor (ER) alpha and beta in uterine leiomyomas, normal myometrium, and endometrium.


METHODS
Prospective study.


METHODS
University hospital.


METHODS
Seventeen premenopausal women who underwent surgery due to symptomatic uterine leiomyomas.


METHODS
None.


METHODS
Quantitative RT-PCR was used for evaluation of ERalpha and ERbeta expression. Results were normalized to total cellular DNA.


RESULTS
Expression of ERalpha and ERbeta did not differ between leiomyomas and myometrium; however, in both tissues, expression of ERalpha was significantly higher than that of ERbeta. Estrogen receptor-alpha expression in endometrium was lower than in leiomyomas and myometrium. In leiomyomas and endometrium, correlations between expression of ERalpha and ERbeta were found.


CONCLUSIONS
Uterine leiomyomas, myometrium, and endometrium display distinct patterns of ER expression.
Jakimiuk
Artur J
AJ
Second Department of Gynecology, University School of Medicine, Lublin, Poland. yakimiuk@yahoo.com
Bogusiewicz
Michal
M
Tarkowski
Rafal
R
Dziduch
Piotr
P
Adamiak
Aneta
A
Wróbel
Andrzej
A
Haczyński
Józef
J
Magoffin
Denis A
DA
Jakowicki
Jerzy A
JA
D000328:Adult; D004717:Endometrium / Q000737:chemistry; D047628:Estrogen Receptor alpha / Q000032:analysis* / Q000235:genetics; D047629:Estrogen Receptor beta / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000737:chemistry*; D008875:Middle Aged; D009215:Myometrium / Q000737:chemistry; D017697:Premenopause / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000737:chemistry*
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger