Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1.
Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0512, USA. frank.loeffler@ce.gatech.edu.
Rosa
R
Krajmalnik-Brown
Tina
T
Hölscher
Ivy N
IN
Thomson
F Michael
FM
Saunders
Kirsti M
KM
Ritalahti
Frank E
FE
Löffler
D000595:Amino Acid Sequence; D001483:Base Sequence; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D016386:Chromosome Walking; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D014752:Vinyl Chloride / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D004269:DNA, Bacterial; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D014752:Vinyl Chloride