icaA is not a useful diagnostic marker for prosthetic joint infection.
A collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaA could be used to distinguish between pathogens and nonpathogens. All Staphylococcus aureus prosthetic joint infection isolates (n = 55) were icaA positive. A total of 46% (20 out of 44) of coagulase-negative staphylococcal prosthetic joint infection isolates were icaA positive, and 30% (7 out of 23) of arthroplasty-associated non-prosthetic joint infection-associated coagulase-negative staphylococcal isolates were icaA positive (P = 0.23). Certain coagulase-negative Staphylococcus species appeared more likely to be isolated as either arthroplasty-associated non-prosthetic joint infection-associated isolates (e.g., Staphylococcus warneri and Staphylococcus hominis) or pathogens (e.g., Staphylococcus lugdunensis). The presence of icaA in a coagulase-negative staphylococcal isolate associated with an arthroplasty is not a useful diagnostic indicator of pathogenicity.
Department of Biochemistry, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Kristi L
KL
Frank
Arlen D
AD
Hanssen
Robin
R
Patel
D001178:Arthroplasty; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers; D003030:Coagulase / Q000378:metabolism; D006622:Hip Prosthesis / Q000382:microbiology; D006801:Humans; D007720:Knee Prosthesis / Q000382:microbiology; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity
D001426:Bacterial Proteins; D015415:Biomarkers; D003030:Coagulase; D017351:N-Acetylglucosaminyltransferases