An antioxidant tetrapeptide UPF1 in rats has a neuroprotective effect in transient global brain ischemia.
Different glutathione analogues have potential to maintain or increase tissue glutathione level and to scavenge the reactive oxygen species. We designed and synthesized a novel non-toxic glutathione analogue, named UPF1, which possessed 60-fold higher hydroxyl radical scavenger efficiency in vitro, compared with glutathione itself, and investigated the effects of UPF1 on a four-vessel occlusion model of rats. The UPF1 was administered via the jugular vein in two separate experiments at two time points: 20 min before global brain ischemia and immediately before reperfusion. In both cases the number of pyramidal cells surviving in the subfield of CA1 at the dorsal hippocampus in the UPF1-treated groups of rats was twice as high as in the vehicle group.
Põder
P
P
Department of Biochemistry, European Centre of Molecular and Clinical Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia. priit.poder@kliinikum.ee
Zilmer
M
M
Starkopf
J
J
Kals
J
J
Talonpoika
A
A
Pulges
A
A
Langel
U
U
Kullisaar
T
T
Viirlaid
S
S
Mahlapuu
R
R
Zarkovski
A
A
Arend
A
A
Soomets
U
U
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002544:Cerebral Infarction / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000150:complications*; D008297:Male; D020365:RNA Helicases / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control; D013997:Time Factors
D000975:Antioxidants; D020365:RNA Helicases; D005978:Glutathione