title: Immunoelectron microscopic analysis of cornified cell envelopes and antigen retrieval.
abstract: In this chapter, postembedding immunoelectron microscopy methods for studies of cornified cell envelopes are provided. Human epidermal tissue samples are used as the material. The samples are cryo-fixed without chemical fixation, freeze-substituted at a low temperature, and embedded in Lowicryl K11M resin. For immunostaining, colloidal gold-conjugated secondary antibodies are used. Methods for retrieval of masked epitopes are also described.
authors.affiliation: Department of Dermatology, Asahikawa Medical College, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Ishida-Yamamoto
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002462:Cell Membrane / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D006801:Humans; D015603:Keratinocytes / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D011498:Protein Precursors / Q000378:metabolism*
chemical_list: D000180:Acrylic Resins; D008565:Membrane Proteins; D011498:Protein Precursors; C064554:loricrin; C050066:Lowicryl K11M; C032253:involucrin