[Genetic diversity of rare and endangered plant Magnolia amoena].
The genetic variations within and between eleven natural populations and one cultured population of Magnolia amoena from different distribution regions were investigated at the DNA level by employing RAPD. Out of 40 random primers, fourteen random primers were screened which could generate highly reproducible and clear RAPD fragments for further population analysis. With these primers, a total of 94 discernible DNA fragments were obtained and 23 (24.4%) were polymorphic, which indicated that low levels of genetic variation existed in the investigated populations. In addition, UPGMA map were made according to the genetic similarity and distance of the twelve populations calculated in this article. The result showed that the genetic diversity between individuals within population was lower than that between populations. The individual genetic differentiation might be relation to their geographic distribution. The low total genetic diversity of Magnolia amoena was one reason for its endangerment.
Liu
Dengyi
D
Biodiversity Research Center, The Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources in Anhui, Anhui Normal University, Wuhu 241000, China. ldy@mail.ahnu.edu.cn
Chu
Ling
L
Yang
Yuehong
Y
D002681:China; D003247:Conservation of Natural Resources; D014644:Genetic Variation; D031566:Magnolia / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique