The Th2 response as monitored by CRTH2 or CCR3 expression is severely decreased during septic shock.
The shift of T lymphocytes toward a Th2 profile during septic shock has been established on the basis of in vitro cytokine production. In the present study, the Th2 response was investigated at the level of cell surface marker expression (whole blood flow cytometry). In 58 patients with septic shock, we observed a reduced CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells) expression on Th2 lymphocytes and regulatory T cells in comparison with 39 healthy volunteers. Eosinophils, which constitutively express CRTH2 in healthy individuals, also exhibited low levels of CRTH2 in patients. In addition, eosinophil CCR3 expression (eotaxin receptor, type 2 chemokine) was strongly correlated with CRTH2, suggesting thus an extended modulation of Th2 related molecules. Importantly, the persistence over time of low levels of CRTH2 or CCR3 expression was found in nonsurvivors. We hypothesize that the restoration of CRTH2/CCR3 expression may be an indicator for optimal recovery after septic shock.
Venet
Fabienne
F
Flow Cytometry Unit, Immunology Laboratory and Intensive Care Units, Lyon-Sud University Hospital, CEDEX F-69495 Pierre-Bénite, France.
Lepape
Alain
A
Debard
Anne-Lise
AL
Bienvenu
Jacques
J
Bohé
Julien
J
Monneret
Guillaume
G
D000368:Aged; D000369:Aged, 80 and over; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000276:immunology / Q000378:metabolism*; D012772:Shock, Septic / Q000276:immunology* / Q000378:metabolism*; D015996:Survival Rate; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
C515350:CCR3 protein, human; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; C036293:prostaglandin D2 receptor