Description of a novel HLA-B allele, B*5613, identified during HLA-typing using sequence-specific oligonucleotide hybridization and sequence-specific amplification.
Here, we report on the characterization of a novel human leukocyte antigen (HLA)-B allele, B*5613. The allele was identified in an adult male from North Africa who was suffering from sickle cell anemia. HLA-B*5613 most closely matches to B*5601 differing only by a substitution of three nucleotides of codon 180. Due to this substitution, low-resolution HLA-typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. DNA sequencing confirmed a variation of codon 180 (CTG-->GAC) resulting in an amino acid substitution Leu156Asp.
Hoppe
B
B
Institute of Transfusion Medicine, Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Germany. berthold.hoppe@charite.de
Heymann
G A
GA
Schoenemann
C
C
Nagy
M
M
Kiesewetter
H
H
Salama
A
A
D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens; C497985:HLA-B*56:13 antigen; C056079:HLA-B56 antigen