title: Hematopoietic gene expression profile in zebrafish kidney marrow.
abstract: The zebrafish kidney marrow is considered to be the organ of definitive hematopoiesis, analogous to the mammalian bone marrow. We have sequenced 26,143 ESTs and isolated 304 cDNAs with putative full-length ORF from a zebrafish kidney marrow cDNA library. The ESTs formed 7,742 assemblies, representing both previously identified zebrafish ESTs (56%) and recently discovered zebrafish ESTs (44%). About 30% of these EST assemblies have orthologues in humans, including 1,282 disease-associated genes in the Online Mendelian Inheritance in Man (OMIM) database. Comparison of the effective and regulatory molecules related to erythroid functions across species suggests a good conservation from zebrafish to human. Interestingly, both embryonic and adult zebrafish globin genes showed higher homology to the human embryonic globin genes than to the human fetal/adult ones, consistent with evo-devo correlation hypothesis. In addition, conservation of a whole set of transcription factors involved in globin gene switch suggests the regulatory network for such remodeling mechanism existed before the divergence of the teleost and the ancestor of mammals. We also carried out whole-mount mRNA in situ hybridization assays for 493 cDNAs and identified 80 genes (16%) with tissue-specific expression during the first five days of zebrafish development. Twenty-six of these genes were specifically expressed in hematopoietic or vascular tissues, including three previously unidentified zebrafish genes: coro1a, nephrosin, and dab2. Our results indicate that conserved genetic programs regulate vertebrate hematopoiesis and vasculogenesis, and support the role of the zebrafish as an important animal model for studying both normal development and the molecular pathogenesis of human blood diseases.
authors.affiliation: State Key Lab for Medical Genomics, Shanghai Institute of Hematology, Ruijin Hospital Affiliated to Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Song
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Sun
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Sheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Jiang
authors.forename: Hui-Yong
authors.initials: HY
authors.lastname: Fan
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: John P
authors.initials: JP
authors.lastname: Kanki
authors.forename: Ting Xi
authors.initials: TX
authors.lastname: Liu
authors.forename: A Thomas
authors.initials: AT
authors.lastname: Look
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D005075:Biological Evolution; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004920:Erythropoiesis / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D008666:Metalloendopeptidases; C106352:nephrosin