title: Initial experience using histidine-tryptophan-ketoglutarate solution in clinical pancreas transplantation.
abstract: BACKGROUND
  The colloid-based University of Wisconsin (UW) preservation solution has been used extensively in clinical pancreas transplantation. Experimental studies support the use of the crystalloid-based histidine-tryptophan-ketoglutarate (HTK) preservation solution for this purpose.
  
  
  OBJECTIVE
  We report our initial experience with HTK for pancreas allograft preservation and compare this to a contemporary experience with UW solution in conventional multiorgan deceased donors (<50 yr).
  
  
  METHODS
  Retrospectively collected information on 33 pancreas transplants between September 2001 and October 2002 were analyzed for early graft function and complications up to 30 d after procurement and storage in either HTK or UW solutions. During multi-organ recovery, either UW solution (4-5 L) or HTK solution (8-10 L) was used for aortic perfusion and subsequent back-table flush and storage. Exocrine drainage of 31 pancreas allografts was enteric, while the bladder was used for drainage in two cases. Patient outcomes were analyzed according to the preservation solution used. Sixteen pancreata were used in combination with a kidney allograft (SPK), seven were used in patients after prior kidney transplantation (PAK), while 10 were used in patients who were not in renal failure (PTA).
  
  
  RESULTS
  The UW group consisted of 17 patients (10 SPK, three PAK, four PTA) with a mean donor age of 29.5 +/- 10.7, and a mean cold ischemia time of 15.1 +/- 2.1 h. The mean post-transplant pancreas and kidney function on days 1 and 10 were amylase (315 and 99 IU/L), lipase (1727 and 346 IU/L), glucose (121 and 100 mg/dL) and creatinine (5.01 and 1.77 mg/dL). Patient and graft survival was 100% at 1-month post transplant. In the HTK group there were 16 patients (six SPK, four PAK, six PTA) with a mean donor age 21.9 +/- 5.7 and a mean cold ischemia time 14.0 +/- 1.3 h. The mean post-transplant pancreas and kidney function on days 1 and 10 were amylase (588 and 126 IU/L), lipase (4711 and 441 IU/L), glucose (97 and 109 mg/dL) and creatinine (5.28 and 2.42 mg/dL). Patient survival was 100% while graft survival was 94% at 1-month post-transplant.
  
  
  CONCLUSIONS
  Early graft function and complications are comparable with HTK and UW solutions for pancreas allograft preservation.
authors.affiliation: Division of Transplantation Surgery, University of Pittsburgh and the Thomas E. Starzl Transplantation Institute, Pittsburgh, PA 15213-3236, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Potdar
authors.forename: Sayeed
authors.initials: S
authors.lastname: Malek
authors.forename: Bijan
authors.initials: B
authors.lastname: Eghtesad
authors.forename: Ron
authors.initials: R
authors.lastname: Shapiro
authors.forename: Amit
authors.initials: A
authors.lastname: Basu
authors.forename: Kusum
authors.initials: K
authors.lastname: Patel
authors.forename: Brian
authors.initials: B
authors.lastname: Broznick
authors.forename: John
authors.initials: J
authors.lastname: Fung
mesh_terms: D000241:Adenosine; D000328:Adult; D000493:Allopurinol; D005260:Female; D005947:Glucose; D005978:Glutathione; D006801:Humans; D007328:Insulin; D008297:Male; D008353:Mannitol; D019188:Organ Preservation Solutions; D010179:Pancreas; D016035:Pancreas Transplantation / Q000502:physiology*; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D012189:Retrospective Studies
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose