title: Involvement of CD8+ T-cells in exacerbation of corneal scarring in mice.
abstract: OBJECTIVE
  To determine the specific immune responses involved in the exacerbation of corneal scarring induced by HSV-1 in gK vaccinated mice.
  
  
  METHODS
  BALB/c mice were vaccinated with HSV-1 glycoprotein K (gK) and ocularly challenged with HSV-1. Infiltration into the cornea of T cells and macrophages was monitored by immunocytochemistry, and the effect of depletion of CD4+ T-cells, CD8+ T-cells, or macrophages on corneal scarring was determined.
  
  
  RESULTS
  Following ocular challenge, CD4+ and CD8+ T-cells and macrophages were more abundant in the corneas of gK-vaccinated mice than in the corneas of mock vaccinated mice. Depletion of CD8+ T-cells, but not of CD4+ T-cells or macrophages, reduced the severity of corneal scarring in gK-vaccinated mice.
  
  
  CONCLUSIONS
  We have shown that gK vaccination causes an overall increase in T cells and macrophages in the cornea after ocular HSV-1 challenge. The immunopathology induced by gK vaccination appears to be related to CD8+ T-cell activity, as depletion of these cells, but not other immune cells, reduced corneal scarring.
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, CSMC Burns & Allen Research Institute, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.
authors.forename: Yanira
authors.initials: Y
authors.lastname: Osorio
authors.forename: Steve
authors.initials: S
authors.lastname: Cai
authors.forename: Florence M
authors.initials: FM
authors.lastname: Hofman
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Brown
authors.forename: Homayon
authors.initials: H
authors.lastname: Ghiasi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000917:Antibody Formation; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000473:pathology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D002921:Cicatrix / Q000276:immunology* / Q000473:pathology / Q000821:virology; D004002:Clodronic Acid / Q000494:pharmacology; D003315:Cornea / Q000473:pathology; D003316:Corneal Diseases / Q000276:immunology* / Q000473:pathology / Q000821:virology; D005123:Eye / Q000821:virology; D005260:Female; D022301:Herpes Simplex Virus Vaccines / Q000276:immunology; D018259:Herpesvirus 1, Human; D007111:Immunity, Cellular; D016849:Keratitis, Herpetic / Q000150:complications / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D022301:Herpes Simplex Virus Vaccines; C072448:UL53 protein, Human herpesvirus 1; D014764:Viral Proteins; D004002:Clodronic Acid