title: New insights into the proton-dependent oxygen affinity of Root effect haemoglobins.
abstract: A long-standing puzzle with regard to protein structure/function relationships is the proton-dependent modification of haemoglobin (Hb) structure that causes oxygen to be unloaded from Root effect Hbs into the swim bladders and eyes of fish even against high oxygen pressure gradients. Although oxygen unloading in Root effect Hbs has generally been attributed to proton-dependent stabilization of the T-state, protonation of Root effect Hbs can alter their ligand affinities in both R- and T-state conformations and either stabilize the T-state or destabilize the R-state. The C-terminal residues that are so important in the Bohr effect of human Hb appear to be involved in the Root effects of some fish Hbs and not in others, indicating that several evolutionary pathways have resulted in expression of highly pH-dependent Hbs. New data are presented that show surprising similarities in the pH- and anion-dependence of sulfhydryl group reactivity and anaerobic oxidation of human and fish Hbs. The available evidence supports the concept that in both Bohr effect and Root effect Hbs a large steric component acts in addition to quaternary shifts between R and T conformations to regulate ligand affinity. Allosteric effectors moderate these steric effects within both R- and T-state conformations and allow for an elegant match between Hb function and the wide-ranging physiological needs of diverse organisms.
authors.affiliation: Nicholas School of the Environment and Earth Science, Duke University Marine Laboratory, Beaufort, NC 28516, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bonaventura
authors.forename: A L
authors.initials: AL
authors.lastname: Crumbliss
authors.forename: R E
authors.initials: RE
authors.lastname: Weber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005399:Fishes / Q000502:physiology*; D006441:Hemoglobin A / Q000502:physiology; D006454:Hemoglobins / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010100:Oxygen / Q000502:physiology*; D011487:Protein Conformation; D011522:Protons; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000502:physiology; D014413:Tuna / Q000502:physiology
chemical_list: D006454:Hemoglobins; D011522:Protons; D013438:Sulfhydryl Compounds; D006441:Hemoglobin A; D010100:Oxygen