[CML cell line K562 cell apoptosis induced by mangiferin].
This study was aimed to investigate the effects and the mechanism of mangiferin on chronic myeloid leukemia cell lines K562 cells in vitro. The antiproliferation effects of mangiferin on K562 leukemia cells were tested by tetrazolium salt (MTT) method; the apoptosis induced by mangiferin on K562 cell line was explored by means of cell morphology, DNA gel electrophoresis and flow cytometry. The changes in bcr/abl gene expression was detected by using reverse transcriptase (RT)-PCR. The results showed that five different concentrations of mangiferin (25 - 200 micromol/L) dose-dependently and time-dependently inhibited the proliferation of K562 cells, and induced apoptosis in K562 cell line. RT-PCR revealed that bcr/abl gene expression was down-regulated when K562 cells had been treated with different concentrations of mangiferin. In conclusion, mangiferin remarkably inhibits the proliferation of K562 leukemia cells in vitro, and induces apoptosis in K562 cell line probably through down-regulation of bcr/abl gene expression.
Peng
Zhi-Gang
ZG
Department of Hematology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China. zgpeng@Hotmail.com
Luo
Jun
J
Xia
Ling-Hui
LH
Chen
Yan
Y
Song
San-Jun
SJ
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005434:Flow Cytometry; D016313:Genes, abl; D006801:Humans; D020014:K562 Cells; D044004:Xanthones / Q000494:pharmacology*
D044004:Xanthones; C013592:mangiferin