title: Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.
abstract: Mouse autocrine motility factor (mAMF), a tumour-secreted cytokine that stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 69.97, b = 115.88, c = 73.27 A, beta = 101.76 degrees . There are two subunits (one dimer) per asymmetric unit. Complexes with four-, five- and six-carbon carbohydrate phosphate inhibitors have also been crystallized. The crystals diffract to at least 1.8 A resolution and are suitable for X-ray structure analyses at high resolution.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Naba
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Katsura
authors.initials: K
authors.lastname: Shiraiwa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kusakabe
authors.forename: Tatsuyoshi
authors.initials: T
authors.lastname: Funasaka
authors.forename: Arayo
authors.initials: A
authors.lastname: Haga
authors.forename: Hisamitsu
authors.initials: H
authors.lastname: Nagase
authors.forename: Avraham
authors.initials: A
authors.lastname: Raz
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice
chemical_list: D005956:Glucose-6-Phosphate Isomerase