Expression of a cascading genetic network within liposomes.
Liposomes have long been used as possible compartments for artificial cells, and it has been shown that liposomes can sustain various types of biochemical reactions. To elevate the degree of molecular complexity of the system in liposomes, we have constructed a two-stage genetic network encapsulated in liposomes. This two-stage genetic network was constructed with the plasmid pTH, in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP). We show that the two-stage genetic network constructed in a cell-free expression system is functional within liposomes.
Ishikawa
Keitaro
K
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Sato
Kanetomo
K
Shima
Yasufumi
Y
Urabe
Itaru
I
Yomo
Tetsuya
T
D001483:Base Sequence; D002474:Cell-Free System; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008081:Liposomes; D010957:Plasmids / Q000235:genetics; D014764:Viral Proteins
D017931:DNA Primers; D008081:Liposomes; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases