Reporter cell lines for the detection of herpes simplex viruses.
Virus culture has played significant roles in basic and clinical virology, with a number of advantages that cannot be attainable by modern molecular techniques. However, virus culture is generally a slower process, as it inevitably takes the period of a full replication cycle of a given virus. A genetically modified cell culture with a virus-inducible marker is described here, using a frequently isolated DNA virus (herpes simplex virus) as a model. The assay system relies on expression of the reporter gene driven by a specific viral promoter that is triggered early in the course of viral infection. The reporter gene employed was green fluorescent protein (GFP) or secreted alkaline phosphatase (SEAP), whose assays offer real-time detection or quantification, respectively. This cell-based assay is simple, rapid, sensitive, specific, and quantitative and serves as a phenotypic method for determination of antiviral susceptibilities.
Kung
Szu-Hao
SH
Faculty of Medical Technology and Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.
D000818:Animals; D002522:Chlorocebus aethiops; D017930:Genes, Reporter; D006561:Herpes Simplex / Q000175:diagnosis* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics; D018139:Simplexvirus / Q000378:metabolism*; D014709:Vero Cells
C100671:ICP10 protein, herpes simplex virus type 2; D012264:Ribonucleotide Reductases; D017346:Protein Serine-Threonine Kinases