Fish protein improves the total antioxidant status of streptozotocin-induced diabetes in spontaneously hypertensive rat.
BACKGROUND
We measured the effects of fish protein (FP) on blood pressure, glycemia and antioxidant status in spontaneously hypertensive (SH) rats with streptozotocin-induced diabetes (STZ).


METHODS
Two groups of 12 rats each were fed 20% casein (C) or FP for 2 months. The total antioxidant status of blood and organs (liver, kidney and heart) was measured by the KRL test. Antioxidant enzyme activities (G-Px, G-Red, and SOD) and antioxidant substances (GSH, NO) were determined in organs, and vitamin C in plasma.


RESULTS
FP lowered blood pressure in SH rats, but not in SH-STZ. Blood and plasma antioxidant status increased 35% and 9%, respectively, with FP in SH-STZ compared to SH rats; when compared to C, these values were more enhanced. SOD activity values were elevated with FP in SH-STZ rats, compared to the C diet, regardless of organ. Higher kidney NO and heart GSH values were noted in SH-STZ rats than SH. In SH rats fed FP, the GSH value was 2.26 times higher in liver, and NO was 3 times higher in heart. Higher NO was noted in kidney (1.84 times) and heart (1.91 times), GSH in heart (1.79 times), and vitamin C in plasma (+46%) in SH-STZ rats with FP than with C.


CONCLUSIONS
Fish protein has a beneficial effect on blood pressure in SH rats but not in SH-STZ, and plays an important role in antioxidative defense. This protein may be useful in future treatments of such diseases as diabetes and hypertension.
Boukortt
Farida Ouda
FO
Laboratoire de Nutrition Clinique et Métabolique, Faculté des Sciences, Université d'Oran Es-Sénia, Oran, Algeria.
Girard
Aurélie
A
Prost
Josiane L
JL
Ait-Yahia
Dalila
D
Bouchenak
Malika
M
Belleville
Jacques
J
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001786:Blood Glucose / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects*; D002364:Caseins / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology; D029941:Fish Proteins / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D005980:Glutathione Reductase / Q000097:blood / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D011918:Rats, Inbred SHR; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D002364:Caseins; D029941:Fish Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase