title: Determination of the functional unit of the cystic fibrosis transmembrane conductance regulator chloride channel. One polypeptide forms one pore.
abstract: The magnitudes and distributions of subconductance states were studied in chloride channels formed by the wild-type cystic fibrosis transmembrane conductance regulator (CFTR) and in CFTRs bearing amino acid substitutions in transmembrane segment 6. Within an open burst, it was possible to distinguish three distinct conductance states referred to as the full conductance, subconductance 1, and subconductance 2 states. Amino acid substitutions in transmembrane segment 6 altered the duration and probability of occurrence of these subconductance states but did not greatly alter their relative amplitudes. Results from real time measurements indicated that covalent modification of single R334C-CFTR channels by [2-(trimethylammonium)ethyl]methanethiosulfonate resulted in the simultaneous modification of all three conductance levels in what appeared to be a single step, without changing the proportion of time spent in each state. This behavior suggests that at least a portion of the conduction path is common to all three conducting states. The time course for the modification of R334C-CFTR, measured in outside-out macropatches using a rapid perfusion system, was also consistent with a single modification step as if each pore contained only a single copy of the cysteine at position 334. These results are consistent with a model for the CFTR conduction pathway in which a single anion-conducting pore is formed by a single CFTR polypeptide.
authors.affiliation: School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332-0230, USA.
authors.forename: Zhi-Ren
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Guiying
authors.initials: G
authors.lastname: Cui
authors.forename: Xuehong
authors.initials: X
authors.lastname: Liu
authors.forename: Binlin
authors.initials: B
authors.lastname: Song
authors.forename: David C
authors.initials: DC
authors.lastname: Dawson
authors.forename: Nael A
authors.initials: NA
authors.lastname: McCarty
mesh_terms: D000818:Animals; D002712:Chlorides; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry* / Q000378:metabolism; D015640:Ion Channel Gating; D017136:Ion Transport; D008956:Models, Chemical; D016297:Mutagenesis, Site-Directed; D018408:Patch-Clamp Techniques; D010455:Peptides; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
chemical_list: D002712:Chlorides; D010455:Peptides; D019005:Cystic Fibrosis Transmembrane Conductance Regulator