title: Characterization of blood vessels in bone marrow from patients with chronic myeloid leukemia and polycythemia vera.
abstract: Angiogenesis is a feature of hematological malignancies which may provide prognostic information. However, there is, as yet, no established marker for leukemia-associated vessels in bone marrow. In this study, immunohistochemical stainings for von Willebrand factor (vWf), CD34, Tie-2, angiomodulin, glycodelin, cycloxygenase-2 (Cox-2) and endoglin were compared in order to identify the bone marrow vasculature. Chronic myeloid leukemia (CML), a disease displaying intense angiogenesis, and polycythemia vera (PV), a disease with a low microvascular density (MVD), were studied, as well as normal bone marrow. Only vWf, CD34 and Tie-2 stained the bone marrow endothelium. Although more vessels were stained for vWf than for CD34, there was no evidence that vWf stained more disease-associated vessels. In double staining, Tie-2 co-localized with CD34, but vessels staining only for Tie-2 were also found. However, the number of Tie-2-positive vessels did not correlate to either the MVD or the disease. Angiomodulin, glycodelin, Cox-2 and endoglin did not stain vessel-like structures. In conclusion, estimating the MVD by means of CD34 staining appears to be the most reliable method, but none of the tested molecules qualified as a specific marker for leukemia-associated vessels in the bone marrow.
authors.affiliation: Department of Medicine, Division of Hematology, Huddinge University Hospital, SE-141 86 Stockholm, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Zetterberg
authors.forename: L G
authors.initials: LG
authors.lastname: Lundberg
authors.forename: J
authors.initials: J
authors.lastname: Palmblad
mesh_terms: D000368:Aged; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood*; D001808:Blood Vessels / Q000737:chemistry; D001853:Bone Marrow / Q000098:blood supply*; D001854:Bone Marrow Cells / Q000737:chemistry; D051546:Cyclooxygenase 2; D000071063:Endoglin; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D011087:Polycythemia Vera / Q000175:diagnosis*; D011257:Pregnancy Proteins / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000378:metabolism; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C064206:ENG protein, human; D000071063:Endoglin; D000071816:Glycodelin; D006023:Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; C086009:tumor-derived adhesion factor; D014841:von Willebrand Factor; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D042787:Receptor, TIE-2