title: Expression of a cascading genetic network within liposomes.
abstract: Liposomes have long been used as possible compartments for artificial cells, and it has been shown that liposomes can sustain various types of biochemical reactions. To elevate the degree of molecular complexity of the system in liposomes, we have constructed a two-stage genetic network encapsulated in liposomes. This two-stage genetic network was constructed with the plasmid pTH, in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP). We show that the two-stage genetic network constructed in a cell-free expression system is functional within liposomes.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Kanetomo
authors.initials: K
authors.lastname: Sato
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Shima
authors.forename: Itaru
authors.initials: I
authors.lastname: Urabe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D001483:Base Sequence; D002474:Cell-Free System; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D008081:Liposomes; D010957:Plasmids / Q000235:genetics; D014764:Viral Proteins
chemical_list: D017931:DNA Primers; D008081:Liposomes; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases