Laboratory diagnosis of four recent sporadic cases of community-acquired SARS, Guangdong Province, China.
Four cases of severe acute respiratory syndrome (SARS) that occurred from December 16, 2003, to January 8, 2004, in the city of Guangzhou, Guangdong Province, China, were investigated. Clinical specimens collected from these patients were tested by provincial and national laboratories in China as well as members of the World Health Organization SARS Reference and Verification Laboratory Network in a collaborative effort to identify and confirm SARS-associated coronavirus (SARS-CoV) infection. Although SARS-CoV was not isolated from any patient, specimens from three patients were positive for viral RNA by reverse transcription-polymerase chain reaction assay, and all patients had detectable rises in SARS-CoV-specific antibodies. This study shows the effectiveness of a collaborative, multilaboratory response to diagnose SARS.
Liang
Guodong
G
Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Chen
Qiuxia
Q
Xu
Jianguo
J
Liu
Yufei
Y
Lim
Wilina
W
Peiris
J S M
JS
Anderson
Larry J
LJ
Ruan
Li
L
Li
Hui
H
Kan
Biao
B
Di
Biao
B
Cheng
Peter
P
Chan
K H
KH
Erdman
Dean D
DD
Gu
Shuyan
S
Yan
Xinge
X
Liang
Weili
W
Zhou
Duanhua
D
Haynes
Lia
L
Duan
Shumin
S
Zhang
Xin
X
Zheng
Han
H
Gao
Yang
Y
Tong
Suxiang
S
Li
Dexin
D
Fang
Ling
L
Qin
Pengzhe
P
Xu
Wenbo
W
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002681:China; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis*
D000914:Antibodies, Viral; D012367:RNA, Viral