title: Distinct pattern of gene expression in pyothorax-associated lymphoma (PAL), a lymphoma developing in long-standing inflammation.
abstract: Pyothorax-associated lymphoma (PAL) is a unique lymphoma developing in the pleural cavity after long-standing pyothorax. They are diffuse large B-cell lymphomas (DLBCLs), frequently with immunoblastic morphology, and show a strong association with Epstein-Barr virus (EBV) infection. In this study, cDNA microarray analysis was performed in six cases with PAL and 12 with nodal DLBCL. Among 5516 informative genes, 348 displayed more than 2-fold difference (higher or lower) of expression level between PAL and nodal DLBCL (P < 0.001). These genes are known to be involved in apoptosis, interferon response, and signal transduction. One of the most differentially expressed genes, IFI27 (interferon-alpha-inducible protein 27) was subjected to quantitative RT-PCR analysis, and increased expression of IFI27 was confirmed. Overexpression of IFI27 was also found in cell lines derived from PAL, but not in other lymphoid cell lines. This study shows that PAL is a distinctive subtype of DLBCL not only in its clinical presentation, but also in its molecular profile.
authors.affiliation: Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Nishiu
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomita
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakatsuka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takakuwa
authors.forename: Norishige
authors.initials: N
authors.lastname: Iizuka
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hoshida
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Ikeda
authors.forename: Keiji
authors.initials: K
authors.lastname: Iuchi
authors.forename: Rempei
authors.initials: R
authors.lastname: Yanagawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
mesh_terms: D000328:Adult; D000368:Aged; D016000:Cluster Analysis; D016724:Empyema, Pleural / Q000150:complications*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008223:Lymphoma / Q000150:complications* / Q000235:genetics*; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000150:complications* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction