Phenotyping the haemostatic system by thrombography--potential for the estimation of thrombotic risk.
The aim of this paper is to review the thrombogram and its use for phenotyping the haemostatic system. The thrombogram can be readily obtained through Calibrated Automated Thrombography (CAT), using a commercially available fluorometer, dedicated software (Thrombinoscope) and a calibrator. Here we explore the possibility to use platelet-rich plasma (PRP) triggered with a low amount of recombinant human tissue factor (approximately 0.5 pM) and also explore the function of the protein C system by adding activated protein C (APC) or soluble recombinant thrombomodulin (TM). Examples are shown: inherited antithrombin (AT) and protein C deficiencies, and antiphospholipid antibodies.
Regnault
Véronique
V
INSERM ERIT-M 0323 et EA 3452, Université Henri Poincaré, Boite Postale 184, 54505 Vandoeuvre-lès-Nancy, France. Veronique.Regnault@nancy.inserm.fr
Hemker
H Coenraad
HC
Wahl
Denis
D
Lecompte
Thomas
T
D000925:Anticoagulants / Q000494:pharmacology; D001778:Blood Coagulation Disorders / Q000175:diagnosis*; D001792:Blood Platelets / Q000378:metabolism*; D002138:Calibration; D005470:Fluorometry / Q000379:methods*; D006487:Hemostasis; D006801:Humans; D010641:Phenotype; D010949:Plasma / Q000276:immunology*; D011486:Protein C / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D012306:Risk; D012984:Software; D013048:Specimen Handling; D018180:Thrombomodulin / Q000737:chemistry; D013925:Thromboplastin / Q000494:pharmacology; D013927:Thrombosis / Q000473:pathology*; D013997:Time Factors
D000925:Anticoagulants; D011486:Protein C; D011994:Recombinant Proteins; D018180:Thrombomodulin; D013925:Thromboplastin