title: Liver function during extracorporeal whole liver perfusion in a pig model of acute ischemic liver failure.
abstract: The shortage of livers for transplant has renewed interest in the potential of temporary liver support such as extra corporeal whole liver perfusion. In an ischemic induced liver failure model we perfused an extra corporeal liver through only a portal vein and assessed the function of this ex vivo liver by using hepatic tests to estimate elimination as well as synthesis capacities. Acute liver failure was performed in five control pigs by a hepatic devascularization associated to an end to side portocaval shunt. In a treated group, 5 to 6 h after this hepatic devascularization, animals were connected to an extra corporeal liver perfused via the portal vein with blood withdrawn from the ischemic liver animal from its portal vein. Devascularization of the liver induced an increase in liver enzymes and ammonia, a drop in the ratio of branched chain amino acids to aromatic amino acids, and a decrease in blood urea and indocyanine green and galactose clearances. In treated animals, urea, amino acid ratio, and clearances increased after the ex vivo liver perfusion. In this group, mean bile production and mean liver oxygen consumption were 13.7 +/- 3.6 ml/h and 16.1 +/- 7.7 ml/min, respectively. In an acute ischemic liver failure pig model, an extra corporeal whole liver perfusion demonstrated detoxification properties as well as synthesis capacities.
authors.affiliation: Surgery Unit, Hôpital Saint Joseph-Saint Luc, Lyon, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Méchet
authors.forename: Franck
authors.initials: F
authors.lastname: Lhuillier
authors.forename: Marie Cécile
authors.initials: MC
authors.lastname: Blanchet
authors.forename: Michel
authors.initials: M
authors.lastname: Pouyet
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Viale
authors.forename: Joelle
authors.initials: J
authors.lastname: Goudable
authors.forename: Guy
authors.initials: G
authors.lastname: Annat
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Scoazec
authors.forename: Olivier
authors.initials: O
authors.lastname: Boillot
authors.forename: Dominique
authors.initials: D
authors.lastname: Liotard
authors.forename: Eric
authors.initials: E
authors.lastname: Merle
authors.forename: Bertrand
authors.initials: B
authors.lastname: Delafosse
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000150:complications; D008099:Liver / Q000098:blood supply / Q000502:physiology; D017093:Liver Failure / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D008111:Liver Function Tests; D006435:Renal Dialysis / Q000379:methods*; D013552:Swine