[Effect of arachidonic acid peroxidation products on the development of gastroesophageal reflux disease].
Oxygen free radicals trigger arachidonic acid peroxidation. The end-products of this reaction are malonyl dialdehyde (MDA) and conjugated dienes (CDs). The pattern of changes of MDA and CD concentrations in serum and esophagus mucosa homogenates were estimated in patients with GERD. The study was conducted on a group of 92 patients. They were divided into a non-esophagitis and co-existent esophagitis subgroup. The presence of inflammation was confirmed by means of pathomorphological examination. Results were verified against upper gastrointestinal tract endoscopy outcomes. The measurements were taken before and 8 weeks after anti-GERD treatment. Results were compared against 20 patients awaiting hernia repair. In both groups MDA serum concentrations before treatment were significantly different from the control group (2.02 nmol/mL in group I and 3.92 nmol/mL in group II). It decreased significantly after treatment in group II (2.72 nmol/mL) and insignificantly in group I. Also DS serum concentrations before treatment was significantly different comparing to the control group (1.34 nmol/mL in group I, and 2.13 nmol/mL in group II). Like MDA, dienes' concentrations decreased significantly after treatment in group II and insignificantly in group I. MDA and DS tissue concentrations were always higher than the serum ones. Both before and after treatment they displayed statistically significant differences between the esophagitis and non-esophagitis groups. Oxygen free radicals measured by means of MDA and DS serum and tissue homogenate concentrations rise significantly in patients with GERD compared to the control group. Arachidonic acid peroxidation products' concentrations were significantly higher in patients with GERD and esophagitis than in the non-esophagitis group.
Modzelewski
Bogdan
B
Klinika Chirurgii Ogólnej i Transplantacyjnej UM w Lodzi.
D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005764:Gastroesophageal Reflux / Q000097:blood / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D010084:Oxidation-Reduction; D026082:Singlet Oxygen / Q000378:metabolism*
D026082:Singlet Oxygen; D016718:Arachidonic Acid; D008315:Malondialdehyde