[In vitro effects of diclofenac and selective cyclooxygenase-2 inhibitors on prostaglandin release from inflamed bursa subacromialis tissue in patients with subacromial syndrome].
BACKGROUND
To compare the in vitro effects of selective COX-2 inhibitors (L-745,337, NS-398 and DFU) and of COX-unspecific diclofenac on release of PGE(2 )and 6-keto-PGF(1alpha) from inflamed bursa subacromialis tissue (IBST) obtained from a total of 35 patients with shoulder impingement syndrome (SIS).


METHODS
Bursal specimens were incubated in the presence of drugs (0.01-1000 microM) for 20 min and 16 h.


RESULTS
After 20 min 10 microM diclofenac significantly inhibited formation of PGE(2) and 6-keto-PGF(1alpha), whereas L-745,337 and NS-398 (10-1000 microM) induced significant inhibition only at concentrations > or =100 microM. In contrast to equimolar diclofenac, DFU (0.01-10 microM) induced no inhibition of bursal PGE(2) release but a dose-dependent, although statistically not significant inhibition after 16 h. The inhibitory potency of diclofenac (0.01-10 microM) was even more increased during long-term incubation showing greater inhibition than DFU at all concentrations studied.


CONCLUSIONS
The data suggest that in IBST in SIS in vitro the majority of PG is generated via the COX-1 pathway.
Knorth
H
H
Abteilung für Rheumaorthopädie im St. Elisabeth-Hospital, Orthopädische Universitätsklinik der Ruhr-Universität Bochum.
Wittenberg
R H
RH
Dorfmüller
P
P
Lebert
R
R
Schmidt
W E
WE
Peskar
B M
BM
Wiese
M
M
Heukamp
M
M
Willburger
R E
RE
D000174:Acromion / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D002062:Bursitis / Q000378:metabolism* / Q000517:prevention & control; D052246:Cyclooxygenase 2 Inhibitors / Q000008:administration & dosage*; D004008:Diclofenac / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011453:Prostaglandins / Q000096:biosynthesis*; D019534:Shoulder Impingement Syndrome / Q000188:drug therapy / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D011453:Prostaglandins; D004008:Diclofenac