Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE).
Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33 degrees C, this was reduced at 37 and 39 degrees C. Concomitantly, the cell growth was arrested at 37 and 39 degrees C compared with that at 33 degrees C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters.
Hosoya
Ken-ichi
K
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan. hosoyak@ms.toyama-mpu.ac.jp
Tomi
Masatoshi
M
Takayama
Megumi
M
Komokata
Yuko
Y
Nakai
Daisuke
D
Tokui
Taro
T
Nishimura
Kenji
K
Ueda
Masatsugu
M
Obinata
Masuo
M
Hori
Satoko
S
Ohtsuki
Sumio
S
Amidon
Gordon L
GL
Terasaki
Tetsuya
T
D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats
D026901:Membrane Transport Proteins; D012333:RNA, Messenger