title: Brugia malayi infection in Meriones unguiculatus: antibody response to recombinant BmR1.
abstract: BmR1 recombinant antigen has previously been shown to demonstrate high sensitivity and specificity in the serological diagnosis of brugian filariasis in humans. In this study, the pattern of recognition of antibody to BmR1 during Brugia malayi infection was investigated by employing Meriones unguiculatus as the experimental model. Thirty two gerbils were infected subcutaneously with 120 L(3); and two control groups each comprising 25 animals were employed. ELISA using BmR1 was used to detect filaria-specific IgG antibodies elicited by the gerbils; using sera collected from the day 1 until day 150 post-inoculation (p.i.). The results showed that BmR1 detected B. malayi infection in gerbils harboring adult worms irrespective of the presence of circulating microfilaria, and was exemplified by positive ELISA results in nine a microfilaraemic animals that harbored live adult worms. The initial time of the antibody recognition was at day 8 p.i. and the antibody titre showed some correlation with adult worm burden.
authors.affiliation: Institute for Research in Molecular Medicine and School of Medical Sciences, Universiti Sains Malaysia, Malaysia.
authors.forename: Boon Huat
authors.initials: BH
authors.lastname: Lim
authors.forename: Rahmah
authors.initials: R
authors.lastname: Noordin
authors.forename: Zurainee Mohamed
authors.initials: ZM
authors.lastname: Nor
authors.forename: Rohana Abdul
authors.initials: RA
authors.lastname: Rahman
authors.forename: Khairul Anuar
authors.initials: KA
authors.lastname: Abdullah
authors.forename: Sivanandam
authors.initials: S
authors.lastname: Sinnadurai
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D017178:Brugia malayi / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis* / Q000276:immunology; D005849:Gerbillinae; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008842:Microfilariae / Q000254:growth & development / Q000302:isolation & purification; D018512:Parasitemia / Q000175:diagnosis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins