A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype.
A novel protein MGC5306 has been identified in yeast-two-hybrid analysis by screening a HeLa cDNA library with a truncated DNA polymerasebeta (polbetaDelta) as bait. The polbetaDelta is expressed in various types of cancers. Co-immunoprecipitation-Western blot analysis confirms not only its interaction with polbetaDelta but also with wild-type polbeta. Binding to polbeta indicates potential function of MGC5306 in repair pathway. Transfection of cells with MGC5306-GFP and Western blot analysis with anti-MGC5306 antibody reveal its nuclear localization. MGC5306 is expressed in human carcinomas and tumor cell lines but not in normal tissues, suggesting MGC5306 is most likely involved in carcinogenesis. An antigrowth activity and modulations of cell cycle events are identified in cells expressing siRNAMGC5306.
Wang
Liming
L
Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, USA.
Bhattacharyya
Nandan
N
Chelsea
Diane M
DM
Escobar
Pedro F
PF
Banerjee
Sipra
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002470:Cell Survival; D019951:DNA Polymerase beta / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000737:chemistry; D010641:Phenotype; D034741:RNA, Small Interfering / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D019951:DNA Polymerase beta