title: Vitamin E-coated dialyzer and antioxidant defense parameters: three-month study.
abstract: Atherosclerotic cardiovascular disease is the most frequent cause of death in patients with end-stage renal disease who have undergone dialysis treatment. Oxidative stress, increased lipid peroxidation, and impaired function of antioxidant systems may contribute to the accelerated development of atherosclerosis in chronic renal failure patients during renal replacement therapy. The aim of this study was to investigate the influence of a vitamin E-coated dialyzer on antioxidant defense parameters in hemodialysis (HD) patients. In 14 HD patients, hemodialysis was performed using a vitamin E-coated dialyzer (Terumo CL-E15NL; Terumo Corporation, Tokyo, Japan) during a 3-month study. In these patients, erythrocyte (ER) antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR) and catalase (CAT), plasma total antioxidant capacity (TAC), RBC glutathione (GSH), plasma malondialdehyde (MDA), plasma, and RBC vitamin E were investigated. Each parameter was measured at the beginning of the study, after the 1st, 2nd, and 3rd month of the study, and 10 weeks after the interruption of the use of vitamin E-coated dialyzer. All HD patients were treated by erythropoietin (EPO) and received vitamin C 50 mg/d, pyridoxine 20 mg/d, and folic acid 5 mg/wk during the entire study. The 3-month treatment with the vitamin E-coated dialyzer led to a significant decrease of plasma MDA level (from 2.85 +/- 0.44 to 2.25 +/- 0.37 micromol/L) and to an increase of plasma TAC, RBC, GSH, and the vitamin E levels both in plasma (from 25.9 +/- 2.8 to 33.6 +/- 3.8 micromol/L) and in the RBCs (from 6.7 +/- 0.8 to 7.4 +/- 0.7 micromol/L) by 30% and 10.5%, respectively. Ten-week interruption of the use of the vitamin E-coated dialyzer led to near initial values of MDA (2.90 +/- 0.28 micromol/L), plasma (28.6 +/- 3.5 micromol/L), and RBC (6.9 +/- 0.7 micromol/L) vitamin E and of other investigated parameters. Statistical analysis of results was performed by conventional methods and analysis of variance. The findings of the current study confirm the beneficial effect of the vitamin E-coated dialyzer against oxidative stress in HD patients.
authors.affiliation: Nephrological Clinic, University Hospital of L. Pasteur, Kosice, Slovak Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Mydlík
authors.forename: Katarína
authors.initials: K
authors.lastname: Derzsiová
authors.forename: Oliver
authors.initials: O
authors.lastname: Rácz
authors.forename: Anna
authors.initials: A
authors.lastname: Sipulová
authors.forename: Eva
authors.initials: E
authors.lastname: Lovásová
authors.forename: Angelika
authors.initials: A
authors.lastname: Molcányiová
authors.forename: Jozefína
authors.initials: J
authors.lastname: Petrovicová
mesh_terms: D000328:Adult; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D018384:Oxidative Stress / Q000187:drug effects*; D006435:Renal Dialysis; D013997:Time Factors; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D008567:Membranes, Artificial; D014810:Vitamin E