title: Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major.
abstract: Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogen.
authors.affiliation: Laboratório de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Henrique
authors.initials: H
authors.lastname: Lanza
authors.forename: Sandra R
authors.initials: SR
authors.lastname: Afonso-Cardoso
authors.forename: Adriano G
authors.initials: AG
authors.lastname: Silva
authors.forename: Danielle R
authors.initials: DR
authors.lastname: Napolitano
authors.forename: Foued S
authors.initials: FS
authors.lastname: Espíndola
authors.forename: Janethe D O
authors.initials: JD
authors.lastname: Pena
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000494:pharmacology; D015415:Biomarkers; D002118:Calcium / Q000494:pharmacology*; D018929:Cell Culture Techniques; D005260:Female; D018320:Leishmania major / Q000276:immunology / Q000472:pathogenicity*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000469:parasitology*; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*
chemical_list: D000953:Antigens, Protozoan; D015415:Biomarkers; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D008274:Magnesium; D002118:Calcium