title: SR-A1, a member of the human pre-mRNA splicing factor family, and its expression in colon cancer progression.
abstract: SR (serine-arginine) proteins are essential pre-mRNA splicing factors. Several SR proteins have been characterized in humans, among them SR-A1. It has been demonstrated by members of our group that the SR-A1 gene is constitutively expressed in most of the human tissues, while its transcription is increased in breast carcinoma cell lines. Moreover, the SR-A1 gene is overexpressed in a set of ovarian tumors, suggesting that it may be involved in the pathogenesis and/or progression of ovarian cancer. Therefore, in the present study we examined the expression of the SR-A1 gene in colon cancer tissues by RT-PCR and found that it is overexpressed as compared to normal mucosa (p=0.01). The SR-A1 gene was expressed more frequently in well-differentiated tumors than those with poor differentiation. Survival curves determined by the Kaplan-Meier method and univariate analysis demonstrated that SR-A1-positivity is associated with a long survival (p=0.044). However, when entered into a Cox multivariate model adjusted for other clinicopathological features studied, SR-A1 expression status was not found to be of independent prognostic significance. To the best of our knowledge, this is the first study examining the expression of the novel gene SR-A1 in colon cancer progression.
authors.affiliation: G. Papanicolaou Research Center of Oncology, Saint Savas Hospital, GR-11522 Athens, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Mathioudaki
authors.forename: Theoni
authors.initials: T
authors.lastname: Leotsakou
authors.forename: Alexandra
authors.initials: A
authors.lastname: Papadokostopoulou
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Paraskevas
authors.forename: Alexandros
authors.initials: A
authors.lastname: Ardavanis
authors.forename: Maroulio
authors.initials: M
authors.lastname: Talieri
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016009:Chi-Square Distribution; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology*; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D012322:RNA Precursors / Q000096:biosynthesis* / Q000235:genetics; D012326:RNA Splicing / Q000502:physiology*; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A; D018709:Statistics, Nonparametric
chemical_list: D005819:Genetic Markers; C495139:MSR1 protein, human; D012322:RNA Precursors; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A