title: [Study on the formaldehyde-induced DNA damage with comet assay].
abstract: Formaldehyde is known as a genotoxic substance. Numerous studies have shown that formaldehyde could induce DNA-DNA and DNA-protein crosslinks. However, scholars have disagreed with each other on the formaldehyde-induced DNA strand breaks. We chose buccal cells as materials to evaluate the genotoxicity of formaldehyde with comet assay--especially for DNA strand breaks. The results showed that formaldehyde of low concentration induced DNA strand breaks, while formaldehyde of high concentration induced DNA-DNA and DNA-protein crosslinks. According to our experimental results, we proposed the concept of the "peak point of break" at which formaldehyde induced DNA strand breaks most.
authors.affiliation: College of the Life Sciences, Central China Normal University, Wuhan 430079.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Zhi Song
authors.initials: ZS
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiao
authors.forename: Han Chao
authors.initials: HC
authors.lastname: Yao
authors.forename: Fei Fei
authors.initials: FF
authors.lastname: Yu
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
mesh_terms: D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D006801:Humans
chemical_list: D005404:Fixatives; D005557:Formaldehyde