Identification of Leishmania strains from Jordan.
The enzymatic profiles of 22 Jordanian Leishmania isolates obtained from humans, Psammomys obesus and Phlebotomus papatasi were determined using starch-gel electrophoresis and a 15-enzyme system. Thirteen of the isolates were typed as L. major and the other nine, all from Mediterranean or sub-Mediterranean regions, as L. tropica. The two zymodemes of L. major encountered, MON-26 and MON-103, differed in terms of purine nucleoside phosphorylase 2. The MON-26 isolates came from the Jordanian plateau whereas those of MON-103 were only collected from the Jordan valley. The four zymodemes of L. tropica observed (MON-7, MON-137, MON-200 and MON-265) were identical for only two of the 15 enzymes studied (i.e. isocitrate dehydrogenase and glucose phosphate isomerase), confirming the high level of enzymatic polymorphism of L. tropica. So far, MON-200 and MON-265 have only been found in Jordan.
Saliba
E K
EK
Department of Biotechnology and Genetic Engineering, Philadelphia University, P.O. Box 1, Amman, 19392, Jordan. salibaek@go.com.jo
Pratlong
F
F
Dedet
J P
JP
Saleh
N
N
Khoury
S A
SA
Oumeish
O Y
OY
Batayneh
O
O
Al-Oran
R
R
D000818:Animals; D004197:Disease Reservoirs; D004592:Electrophoresis, Starch Gel / Q000379:methods; D005849:Gerbillinae / Q000469:parasitology; D006801:Humans; D007303:Insect Vectors; D007527:Isoenzymes / Q000378:metabolism; D007597:Jordan / Q000453:epidemiology; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D018320:Leishmania major / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification; D007895:Leishmania tropica / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000469:parasitology*; D010691:Phlebotomus / Q000469:parasitology
D007527:Isoenzymes