title: Ventral otic cell lines as developmental models of auditory epithelial and neural precursors.
abstract: Conditionally immortal cell lines were established from the ventral otocyst of the Immortomouse at embryonic day 10.5 and selected to represent precursors of auditory sensory neural and epithelial cells. Selection was based upon dissection, tissue-specific markers, and expression of the transcription factor GATA3. Two cell lines expressed GATA3 but possessed intrinsically different genetic programs under differentiating conditions. US/VOT-E36 represented epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells. US/VOT-N33 represented migrating neuroblasts. Under differentiating conditions in vitro the cell lines expressed very different gene expression profiles. Expression of several cell- and tissue-specific markers, including the transcription factors Pax2, GATA3, and NeuroD, differed between the cell lines in a pattern consistent with that observed between their counterparts in vivo. We suggest that these and other conditionally immortal cell lines can be used to study transient events in development against different backgrounds of cell competence.
authors.affiliation: Department of Biomedical Sciences, Addison Building, Western Bank, Sheffield, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Lawoko-Kerali
authors.forename: M
authors.initials: M
authors.lastname: Milo
authors.forename: D
authors.initials: D
authors.lastname: Davies
authors.forename: A
authors.initials: A
authors.lastname: Halsall
authors.forename: R
authors.initials: R
authors.lastname: Helyer
authors.forename: C M
authors.initials: CM
authors.lastname: Johnson
authors.forename: M N
authors.initials: MN
authors.lastname: Rivolta
authors.forename: M A
authors.initials: MA
authors.lastname: Tones
authors.forename: M C
authors.initials: MC
authors.lastname: Holley
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001306:Auditory Pathways / Q000166:cytology / Q000196:embryology / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D004848:Epithelium / Q000196:embryology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051656:KCNQ Potassium Channels; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009925:Organ of Corti / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D011247:Pregnancy; D013136:Spiral Ganglion / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology
chemical_list: D051656:KCNQ Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D002118:Calcium