Expression of interleukin-11 receptor in CD38-positive cells from patients with multiple myeloma.
Interleukin-11, a cytokine with multiple biological activities, has been shown to stimulate the proliferation and to support the long-term growth of human myeloma cell lines. Despite this, no expression of the interleukin-11alpha receptor has so far been demonstrated in myeloma cells. We have investigated the expression of interleukin-11alpha receptor and interleukin-11 at the level of mRNA and protein product in bone marrow mononuclear cells isolated from patients with multiple myeloma using reverse-transcriptase polymerase chain reaction and flow cytometry. The mRNA for interleukin-11alpha receptor and/or the corresponding protein were identified in 9 of 15 patients with multiple myeloma. In contrast, the interleukin-11 was not detected in any of the patients examined.
Tsimanis
Alexander
A
Department of Molecular Cell Biology, Zetiq Technologies, Rehovot Business Park, Gad Feinstein Road, Rehovot, Israel.
Shtalrid
Mordehai
M
Shvidel
Lev
L
Kalinkovich
Alexander
A
Berrebi
Alain
A
Klepfish
Abraham
A
D036541:ADP-ribosyl Cyclase / Q000096:biosynthesis*; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000378:metabolism*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053703:Interleukin-11 Receptor alpha Subunit; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009101:Multiple Myeloma / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018123:Receptors, Interleukin / Q000096:biosynthesis*; D053702:Receptors, Interleukin-11; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D015703:Antigens, CD; C508428:IL11RA protein, human; D053703:Interleukin-11 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1