Elemental and redox analysis of single bacterial cells by x-ray microbeam analysis.
High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.
Kemner
Kenneth M
KM
Environmental Research Division and Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439-4843, USA. kemner@anl.gov
Kelly
Shelly D
SD
Lai
Barry
B
Maser
Joerg
J
O'loughlin
Edward J
EJ
Sholto-Douglas
Deirdre
D
Cai
Zhonghou
Z
Schneegurt
Mark A
MA
Kulpa
Charles F
CF
Nealson
Kenneth H
KH
D001422:Bacterial Adhesion; D018441:Biofilms; D002118:Calcium / Q000032:analysis; D002857:Chromium / Q000032:analysis / Q000494:pharmacology; D004602:Elements; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000032:analysis; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D013052:Spectrometry, X-Ray Emission
D004602:Elements; D002857:Chromium; C074702:chromium hexavalent ion; D010758:Phosphorus; D002118:Calcium