Transcriptional coactivator p300/CBP-associated factor and p300/CBP-associated factor type B are required for normal estrogen response of the mouse uterus.
Mice with targeted gene disruption of one of the estrogen receptor coactivators, p300/CBP-associated factor (PCAF), and its counterpart, PCAF-B, were used to investigate the possible involvement of PCAF and PCAF-B in estrogen receptor-mediated actions in vivo. Among ovariectomized mice that were treated with estrogen, PCAF and PCAF/PCAF-B knockouts showed abnormal growth of the uterus compared with the wild type. The level of c-fos gene expression in the uterus was not induced by estrogen in the knockouts. These observations suggest that PCAF and PCAF-B are required for estrogen-dependent normal growth of the uterus via estrogen receptor-mediated transcriptional regulations.
Inoue
Erina
E
Division of Applied Food Research, National Institute of Health and Nutrition, Shinjuku, Tokyo 162-8636, Japan.
Hanai
Miho
M
Yamada
Kazuhiko
K
Esashi
Takatoshi
T
Yamauchi
Jun
J
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D016762:Genes, fos / Q000235:genetics* / Q000502:physiology; D051548:Histone Acetyltransferases; D051379:Mice; D018345:Mice, Knockout; D010052:Ovariectomy; D011960:Receptors, Estrogen / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014599:Uterus / Q000033:anatomy & histology*; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D004967:Estrogens; D051548:Histone Acetyltransferases; D011960:Receptors, Estrogen; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor