A genetically encoded photocaged amino acid.
We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.
Wu
Ning
N
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Deiters
Alexander
A
Cropp
T Ashton
TA
King
David
D
Schultz
Peter G
PG
D001483:Base Sequence; D002210:Caprylates / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007935:Leucine-tRNA Ligase / Q000235:genetics* / Q000378:metabolism*; D008781:Methyltyrosines / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D010777:Photochemistry; D011506:Proteins / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics
D002210:Caprylates; D008781:Methyltyrosines; D011506:Proteins; D012346:RNA, Transfer, Amino Acyl; C002350:O-methyltyrosine; D006639:Histidine; D013482:Superoxide Dismutase; D007935:Leucine-tRNA Ligase; D003545:Cysteine