title: Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid.
abstract: A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR.
authors.affiliation: Biosensor Laboratory, Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, 136 Chou-Shan Road, Taipei, Taiwan 106, R.O.C.
authors.forename: Hsien-Yi
authors.initials: HY
authors.lastname: Hsiao
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Tsai
authors.forename: Suming
authors.initials: S
authors.lastname: Chen
authors.forename: Bo-Chuan
authors.initials: BC
authors.lastname: Hsieh
authors.forename: Richie L C
authors.initials: RL
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008962:Models, Theoretical; D010756:Phosphoric Acids / Q000737:chemistry*; D012984:Software; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D010756:Phosphoric Acids; D012997:Solvents; D002686:Chitin; D048271:Chitosan; C030242:phosphoric acid