RNA editing and short variant of serotonin 2C receptor mRNA in neuronally differentiated NG108-15 cells.
Two types of serotonin 2C subtype receptor mRNA, receptor-type and short variant, has been reported. The expression of the receptor-type mRNA could be detected as well as the short variant in NG108-15 cells by using a high temperature stable reverse transcriptase and the expression of the receptor-type mRNA was enhanced in drug-induced neuronal differentiated cells. The deleted sequence of the short variant include the RNA editing site by adenosine deaminase. Analysis of the sequence at the editing site revealed that the mRNA of undifferentiated cells was highly edited at sites A and B and that cytosine deaminase activity may also be involved in neuronal differentiation.
Tohda
Michihisa
M
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan. tohdam@ms.toyama-mpu.ac.jp
Sukma
Monrudee
M
Watanabe
Hiroshi
H
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D014644:Genetic Variation; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D044405:Receptor, Serotonin, 5-HT2C / Q000096:biosynthesis / Q000235:genetics*
D020033:Protein Isoforms; D012333:RNA, Messenger; D044405:Receptor, Serotonin, 5-HT2C