title: Detection of Campylobacter or Salmonella in turkey semen and the ability of poultry semen extenders to reduce their concentrations.
abstract: Campylobacter and Salmonella are the most commonly reported pathogens causing foodborne illness in the United States. In turkeys, the potential that semen used for artificial insemination is contaminated with these foodborne pathogens has not been investigated. Because semen on turkey farms is pooled and then used to inseminate multiple hens, contaminated semen could easily spread these bacteria throughout entire flocks via artificial insemination. The objectives of this study were to 1) determine if semen from commercial turkey farms contained these foodborne pathogens and 2) if present, evaluate the efficacy of semen extenders to reduce or eliminate Campylobacter and Salmonella from semen. Semen was collected from randomized pools of ejaculates from 10 to 30 toms per farm from 6 flocks over a 7-wk period and, on occasion, was found to contain Campylobacter, Salmonella, or both. To evaluate the efficacy of semen extenders to reduce or eliminate pathogens, pooled ejaculates were challenged with Campylobacter or Salmonella and treated with commercial poultry extenders containing various concentrations of antibiotics or an antibiotic combination previously demonstrated to remove Campylobacter from mammalian semen. Results demonstrate that commercial turkey semen may contain Campylobacter or Salmonella, and the semen extenders tested either did not reduce the bacteria or reduced but did not eliminate these bacteria from semen. We concluded that semen may be a potential vehicle for Campylobacter transfer to hens, and, if this is true, development of a method for eliminating pathogens in semen before insemination could reduce the risk of colonization.
authors.affiliation: Poultry Production and Product Safety Research Unit, ARS, USDA, Fayetteville, Arkansas 72701, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Donoghue
authors.forename: P J
authors.initials: PJ
authors.lastname: Blore
authors.forename: K
authors.initials: K
authors.lastname: Cole
authors.forename: N M
authors.initials: NM
authors.lastname: Loskutoff
authors.forename: D J
authors.initials: DJ
authors.lastname: Donoghue
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002167:Campylobacter / Q000187:drug effects / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000302:isolation & purification*; D012661:Semen / Q000382:microbiology*; D016896:Treatment Outcome; D014422:Turkeys / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial