title: [Correlation between local hormones and CD36 transcription level in women with polycystic ovary].
abstract: OBJECTIVE
  To detect CD(36) expressions in polycystic ovary (PCO), and to explore its correlation with local androgen and insulin at transcription level.
  
  
  METHODS
  From August 2002 to February 2003, 12 patients with asymmetric PCO, 15 primary or secondary infertile patients without endocrine disorders and 8 polycystic ovary syndrome (PCOS) with bilateral PCO were recruited. Extraction of follicular fluid and detection of testosterone (T), dehydroepiandrosterone sulfate (DHEAS), insulin (INS) and androstenedione (A(2)) were performed. Relative CD(36) mRNA expression level of human ovarian inner thecal cells was analyzed by auto image analysis system (IAS) after RT-PCR.
  
  
  RESULTS
  The level of CD(36) mRNA expression in thecal cells was 0.24 +/- 0.07 in polycystic ovary of PCO group and 0.21 +/- 0.05 in bilateral ovaries of PCOS group, respectively, which were significantly lower than 0.83 +/- 0.13 in normal ovaries (P < 0.01). T and INS levels of follicle fluid in PCO were significantly higher than that in normal ovaries (P < 0.01). T and INS levels of follicle fluid were negatively related to CD(36) mRNA expression of follicular theca interna (r = -0.6810, r = -0.6708, P < 0.01).
  
  
  CONCLUSIONS
  Decrease of scavenger receptor gene CD(36) mRNA may play a role in the pathogenesis of PCO by increasing the level of T and INS in follicular fluid.
authors.affiliation: Department Obstetrics and Gynecology, Affiliated Second Hospital, Zhongshan University, Guangzhou 510120, China.
authors.forename: Liang-qing
authors.initials: LQ
authors.lastname: Yao
authors.forename: Jian-quan
authors.initials: JQ
authors.lastname: Kuang
authors.forename: Dong-zi
authors.initials: DZ
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ying-ming
authors.initials: YM
authors.lastname: He
authors.forename: Guang-yi
authors.initials: GY
authors.lastname: Li
authors.forename: Lu-shi
authors.initials: LS
authors.lastname: Chen
mesh_terms: D000328:Adult; D000735:Androstenedione / Q000378:metabolism; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D013739:Testosterone / Q000378:metabolism*; D013799:Theca Cells / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D007328:Insulin; D012333:RNA, Messenger; D013739:Testosterone; D000735:Androstenedione