Effect of the flavonol quercetin on membrane conductances in rat colonic crypt cells.
The plant polyphenol quercetin was shown to induce Cl- secretion in rat colon. This study was performed to investigate the alterations of membrane conductances in isolated epithelial cells induced by quercetin. Whole-cell patch-clamp recordings were performed in isolated crypts from rat distal colon. In cells of the crypt basis, quercetin significantly hyperpolarized the membrane potential at concentrations > or =3 microM and increased the K+ conductance without visibly altering the Cl- conductance. Thus, quercetin induces Cl- secretion merely by activation of K+ channels in the colon epithelium.
Cermak
Rainer
R
Institute of Animal Nutrition and Physiology, Christian-Albrechts-University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany. cermak@aninut.uni-kiel.de
Wolffram
Siegfried
S
Kuhn
Gisela
G
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D018118:Chloride Channels / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000166:cytology* / Q000187:drug effects*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions
D018118:Chloride Channels; D002712:Chlorides; D015221:Potassium Channels; D012996:Solutions; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D011794:Quercetin