title: Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier.
abstract: Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.
authors.affiliation: Department of Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Teruaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Munenori
authors.initials: M
authors.lastname: Numata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Mariko
authors.initials: M
authors.lastname: Umeda
authors.forename: Ryouji
authors.initials: R
authors.lastname: Karinaga
authors.forename: Masami
authors.initials: M
authors.lastname: Mizu
authors.forename: Kazuya
authors.initials: K
authors.lastname: Koumoto
authors.forename: Taro
authors.initials: T
authors.lastname: Kimura
authors.forename: Shiro
authors.initials: S
authors.lastname: Okumura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakurai
authors.forename: Seiji
authors.initials: S
authors.lastname: Shinkai
mesh_terms: D002452:Cell Count; D002942:Circular Dichroism; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D009844:Oligosaccharides / Q000737:chemistry*; D011061:Poly A / Q000737:chemistry; D011066:Poly C / Q000737:chemistry; D012680:Sensitivity and Specificity; D012566:Sizofiran / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D006027:Glycosides; D046911:Macromolecular Substances; D016376:Oligonucleotides, Antisense; D009844:Oligosaccharides; C008245:lactosides; D011061:Poly A; C015465:poly(dA); D011066:Poly C; D012566:Sizofiran