Involvement of tumor necrosis factor-alpha receptor 1 and tumor necrosis factor-related apoptosis-inducing ligand-(TRAIL) receptor-2/DR-5, but not Fas, in graft injury in live-donor liver transplantation.
The pathway leading to cell death in clinical liver transplantation is not known. Eight liver transplant recipients and eight donors were enrolled in this study. Postoperative serum levels of alanine transferase had significantly increased in the recipients compared with those in the donors. Mild centri-lobular necrosis was observed in only liver tissues taken from the recipients. Tumor necrosis factor (TNF)-R1 and death receptor 5 expression levels had increased in liver tissues taken from the recipients. There were no changes in the levels of Fas/Fas ligand expression in liver tissues from either the donors or recipients. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression was down-regulated in donor liver after hepatectomy and liver allograft after implantation. The results suggest that, although ischemic liver injury was not serious, due to the short ischemia time, TNF and TRAIL signals are associated with liver ischemic injury in live-donor liver transplantation but Fas signal is not.
Tashiro
Hirotaka
H
Second Department of Surgery, Faculty of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551 Hiroshima, Japan. htashiro@hiroshima-u.ac.jp
Itamoto
Toshiyuki
T
Ohdan
Hideki
H
Arihiro
Kouji
K
Tateaki
Yuko
Y
Nakahara
Hideki
H
Ochi
Makoto
M
Hino
Hiroshi
H
Mizunuma
Kazuyuki
K
Hara
Hidetaka
H
Tokita
Daisuke
D
Onoe
Takashi
T
Ishiyama
Kouhei
K
Mitsuta
Hiroshi
H
Sugino
Keizou
K
Asahara
Toshimasa
T
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002675:Child, Preschool; D002763:Cholecystectomy; D053222:Fas Ligand Protein; D005260:Female; D006498:Hepatectomy; D006801:Humans; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D016031:Liver Transplantation; D019520:Living Donors; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009336:Necrosis; D011184:Postoperative Period; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor; D053221:TNF-Related Apoptosis-Inducing Ligand; C505639:TNFRSF10B protein, human; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor