HTRP--an immediate-early gene product induced by HSV1 infection in human embryo fibroblasts, is involved in cellular co-repressors.
The interaction between virus and receptor is a process that mimics physiological ligand binding receptors and induces signal transduction. In the investigation of the interaction between HSV1 (Herpes Simplex virus 1) and human fibroblasts via virus binding to its receptor complex on cellular membranes, the HTRP (human transcription regulator protein), a protein encoded by an immediate-early gene of cellular response against the specific stimulation of HSV1 binding, was cloned from a cDNA library established from early gene response mRNA. The localization of HTRP expressed as a fusion polypeptide with a fluorescent protein in HeLa cells was confirmed to be the nucleus. The results of a yeast two-hybrid experiment indicated that HTRP is indeed involved in the interaction with the SAP (mSin3-associate polypeptide) complex via SAP30. A pull-down test and Western blotting in vitro, and immunoprecipitation in vivo also provided evidence in support of this result. The interaction of HTRP with SAP30 in its conserved domain implies that this protein family, as the products of immediate-early genes, comprise functional molecules involved in the transcriptional regulation of cells, which might be related to the inhibition of some cell survival genes.
Li
Jian-Feng
JF
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, 379 Jiaoling Rd., Kunming, 650118, PR China.
Liu
Long-Ding
LD
Ma
Shao-Hui
SH
Che
Yan-Chun
YC
Wang
Li-Chun
LC
Dong
Cheng-Hong
CH
Zhao
Hong-Ling
HL
Liao
Yun
Y
Li
Qi-Han
QH
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002470:Cell Survival; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology*; D015723:Gene Library; D017781:Genes, Immediate-Early; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006655:Histone Deacetylases / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D009687:Nuclear Proteins; D010455:Peptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C113294:SAP30 protein, human; C500210:SAP30BP protein, human; D014157:Transcription Factors; D012799:Sialyltransferases; C500214:rtST6GalNAc, rainbow trout; D005982:Glutathione Transferase; D006655:Histone Deacetylases