Accumulation of pathogenesis-related type-5 like proteins in phytoplasma-infected garland chrysanthemum Chrysanthemum coronarium.
Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble proteins, determined by Edman degradation, shared high sequence similarities with those of pathogenesis-related type-5 (PR-5) proteins such as tobacco thaumatin-like protein. Accumulation of these six proteins was also found in garland chrysanthemum plants infected by other phytoplasmas. These results demonstrate that phytoplasmal infection induces the accumulation of PR-5 like proteins in garland chrysanthemum plants.
Zhong
Bo-Xiong
BX
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn
Shen
Yan-Wei
YW
D000595:Amino Acid Sequence; D031202:Chrysanthemum / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D045582:Phytoplasma / Q000378:metabolism*; D010935:Plant Diseases; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012984:Software
D010940:Plant Proteins; D011506:Proteins; C073139:osmotin protein, Nicotiana tabacum; C003427:thaumatin protein, plant