Cell kinetic analysis in artificial skin using immunochemical methods.
Cell kinetic studies provide important information on histogenesis in vivo and in vitro. In this regard, specific antibodies to cell cycle-related antigens have been raised and characterized, thus permitting the study of cell kinetics using immunochemical methods. Recent advances in culture technology permitted the generation of human skin equivalents in vitro. We here provide detailed practical procedures for the study of epidermal cell kinetics in a model of artificial skin using immunohistochemistry and flow cytometry. The combined application of both techniques allows a precise detection of tissue growth sites and a quantitative assessment of cell growth. Moreover, simultaneous analysis of differentiation markers and proliferation antigens may be useful to understand molecular mechanisms that regulate tissue growth and development.
Casasco
Andrea
A
Department of Experimental Medicine, University of Pavia, Italy.
Cornaglia
Antonia Icaro
AI
Riva
Federica
F
Casasco
Marco
M
Calligaro
Alberto
A
D002453:Cell Cycle / Q000502:physiology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000276:immunology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017768:Skin, Artificial
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen