title: Preferential target is mitochondria in alpha-mangostin-induced apoptosis in human leukemia HL60 cells.
abstract: Our previous study has shown that alpha-mangostin, a xanthone from the pericarps of mangosteen, induces caspase-3-dependent apoptosis in HL60 cells. In the current study, we investigated the mechanism of apoptosis induced by alpha-mangostin in HL60 cells. Alpha-mangostin-treated HL60 cells demonstrated caspase-9 and -3 activation but not -8, which leads us to assume that alpha-mangostin may mediate the mitochondrial pathway in the apoptosis. Parameters of mitochondrial dysfunction including swelling, loss of membrane potential (deltapsim), decrease in intracellular ATP, ROS accumulation, and cytochrome c/AIF release, were observed within 1 or 2 h after the treatment. On the other hand, alpha-mangostin-treatment did not affect expression of bcl-2 family proteins and activation of MAP kinases. These findings indicate that alpha-mangostin preferentially targets mitochondria in the early phase, resulting in indication of apoptosis in HL60 cells. Furthermore, we examined the structure-activity relationship between xanthone derivatives including alpha-mangostin and the potency of deltapsim-loss in HL60 cells. Interestingly, replacement of hydroxyl group by methoxy group remarkably decreased its potency. It was also shown that the cytotoxicity substantially correlated with deltapsim decrease. These results indicate that alpha-mangostin and its analogs would be candidates for preventive and therapeutic application for cancer treatment.
authors.affiliation: Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. kmatsumo@giib.or.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.forename: Kenji
authors.initials: K
authors.lastname: Ohguchi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ito
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Emi
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Munekazu
authors.initials: M
authors.lastname: Iinuma
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nozawa
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D044004:Xanthones / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D044004:Xanthones; C021053:mangostin