MHC class II-related genes expression in porcine-serum-induced rat hepatic fibrosis.
Genes, especially MHC class II-related genes, expression was examined in porcine-serum (PS)-induced hepatic fibrosis model. Brown-Norway (BN) and Wistar rats were injected intraperitoneally with 0.5 ml sterile porcine serum twice a week for 1, 2, 3, 4, and 8 weeks. Histopathologically, inflammation started at 2 weeks in BN and at 4 weeks in Wistar rats, and hepatic fibrosis developed at 4 weeks in BN rats and at 8 weeks in Wistar rats. Microarray analysis done at 2 and 4 weeks revealed that the expression of MHC class II-related genes and acute phase inflammation-related genes significantly increased at 2 weeks in BN and at 4 weeks in Wistar rats. On the other hand, the expression of some transcription-related genes was down-regulated in both strains. In BN rats, the results of semiquantitative RT-PCR analysis done on four MHC class II-related genes mRNAs corresponded well with those of microarray analysis. MHC class II is considered to be involved in the initiation of PS-induced hepatic fibrosis in rats.
Baba
Yasuko
Y
Department of Veterinary Pathology, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo 113-8657, Japan. aa27157@mail.ecc.u-tokyo.ac.jp
Doi
Kunio
K
D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D005802:Genes, MHC Class II; D008099:Liver / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D051381:Rats; D011914:Rats, Inbred BN; D017208:Rats, Wistar; D044967:Serum / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000097:blood / Q000276:immunology