Intraoperative isolation and processing of BM-derived stem cells.
To improve tissue regeneration of ischemic myocardium, autologous bone marrow-derived stem cells have been injected intramyocardially in five patients undergoing coronary artery bypass grafting and transmyocardial laser revascularization. An innovative method for the intraoperative isolation of CD133(+)-stem cells in less than 3 hours has been established. After induction of general anesthesia, approx. 60-240 ml of bone marrow were harvested from the posterior iliac crest and processed in the operating room under GMP conditions using the automated cell selection device Clini-MACS. Following standard CABG surgery, LASER channels were shot in predefined areas within the hibernating myocardium. Subsequently, autologous CD133(+)-stem cells (1.9-9.7 x 10(6) cells; purity up to 97%) were injected in a predefined pattern around the laser channels. Through the intraoperative isolation of CD133(+)-cells, this effective treatment of ischemic myocardium can be applied to patients scheduled both for elective and for emergency revascularisation procedures.
Ghodsizad
A
A
Department of Cardiovascular and Thoracic Surgery Heinrich-Heine-University of Duesseldorf, Duesseldorf, Germany.
Klein
H-M
HM
Borowski
A
A
Stoldt
V
V
Feifel
N
N
Voelkel
T
T
Piechaczek
Ch
Ch
Burchardt
Er
E
Stockschläder
M
M
Gams
E
E
D000071916:AC133 Antigen; D015703:Antigens, CD; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007432:Intraoperative Period; D010455:Peptides / Q000032:analysis*; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D013997:Time Factors
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides