Alpha-beta chimeric oligo-DNA bearing intercalator-conjugated nucleobase inside the linker sequence remarkably improves thermal stability of an alternate-stranded triple helix.
Novel alpha-beta chimeric oligodeoxynucleotides bearing an intercalator-conjugated nucleobase located at the internal 4-nt linker region were synthesized, and their triplex-stabilizing property was examined. The triple helical DNA formed between the modified chimera DNA and double-stranded DNA exhibited remarkable thermal stability; however, the position of the intercalator-conjugated nucleobase had little influence on the stability. Among the examined, modified chimera DNA bearing the two intercalator-conjugated nucleobases at adjacent positions exhibited the highest stability.
Zafrul Azam
A T M
AT
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan.
Hasegawa
Minoru
M
Moriguchi
Tomohisa
T
Shinozuka
Kazuo
K
D002678:Chimera / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D007364:Intercalating Agents / Q000138:chemical synthesis*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000235:genetics*
D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D004247:DNA