Evaluation of cisplatin-DNA crosslinks formation with UV-C application by the alkaline Comet-assay.
The AIM of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors.


METHODS
DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The RESULTS obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration.


CONCLUSIONS
The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.
Hovhannisyan
Galina G
GG
Department of Genetics and Cytology, Yerevan State University, Yerevan 375049, Armenia.
Haroutunyan
Tigran S
TS
Arutyunyan
Rouben M
RM
D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D002945:Cisplatin / Q000494:pharmacology*; D020552:Comet Assay; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000737:chemistry / Q000187:drug effects; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D004247:DNA; D002945:Cisplatin