Detection of microsatellite instability during somatic embryogenesis of oak (Quercus robur L.).
Five microsatellite loci (QpZAG1/5, QpZAG9, QpZAG36, MSQ4, MSQ13) were used to test for genetic stability of three somatic embryogenic culture lines of Quercus robur L. and plantlets derived therefrom. DNA variation was detected among somatic embryos within all embryogenic lines, whereas no genetic instability was found among the regenerated plants. Two microsatellite loci revealed variation, and a locus-dependent instability was observed. The most polymorphic and useful microsatellite locus for detecting genetic variation was QpZAG9, with 28.5% of the investigated loci being variable.
Wilhelm
E
E
Division of Environment and Life Sciences, Department of Biotechnology, ARC Seibersdorf Research, 2444, Seibersdorf, Austria. eva.wilhelm@arcs.ac.at
Hristoforoglu
K
K
Fluch
S
S
Burg
K
K
D000383:Agriculture / Q000379:methods; D047108:Embryonic Development / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics*; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D029963:Quercus / Q000196:embryology / Q000235:genetics*; D012639:Seeds / Q000196:embryology / Q000235:genetics*