Crystallization and preliminary X-ray crystallographic studies of human 2',3'-cyclic nucleotide 3'-phosphodiesterase.
The catalytic fragment of human 2',3'-cyclic nucleotide 3'-phosphodiesterase (hCNP-CF) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 300 as the precipitating agent. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.39, b = 55.35, c = 78.76 A. There is one molecule per asymmetric unit. The crystals diffract to at least 1.8 A resolution using synchrotron radiation and are suitable for X-ray structure analysis at high resolution.
Sakamoto
Yasumitsu
Y
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Tanaka
Nobutada
N
Ichimiya
Tomomi
T
Kurihara
Tadashi
T
Nakamura
Kazuo T
KT
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571337:CNP protein, human