Diverse odor-conditioned memories require uniquely timed dorsal paired medial neuron output.
Amnesiac mutant flies have an olfactory memory defect. The amn gene encodes a homolog of vertebrate pituitary adenylate cyclase-activating peptide (PACAP), and it is strongly expressed in dorsal paired medial (DPM) neurons. DPM neurons ramify throughout the mushroom bodies in the adult fly brain, and they are required for stable memory. Here, we show that DPM neuron output is only required during the consolidation phase for middle-term odor memory and is dispensable during acquisition and recall. However, we found that DPM neuron output is required during acquisition of a benzaldehyde odor memory. We show that flies sense benzaldehyde by the classical olfactory and a noncanonical route. These results suggest that DPM neurons are required to consolidate memory and are differently involved in memory of a volatile that requires multisensory integration.
Keene
Alex C
AC
Department of Neurobiology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA.
Stratmann
Markus
M
Keller
Andreas
A
Perrat
Paola N
PN
Vosshall
Leslie B
LB
Waddell
Scott
S
D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001362:Avoidance Learning / Q000502:physiology; D001522:Behavior, Animal / Q000502:physiology; D001547:Benzaldehydes / Q000494:pharmacology; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D003213:Conditioning, Psychological / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D051717:Fushi Tarazu Transcription Factors; D006651:Histocytochemistry / Q000379:methods; D018782:Maze Learning / Q000502:physiology; D008568:Memory / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods; D024521:Mushroom Bodies / Q000166:cytology / Q000502:physiology; D016296:Mutagenesis / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D009812:Odorants; D009833:Olfactory Pathways / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism
D001547:Benzaldehydes; D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; C080545:GAL4-ftzQ protein, Drosophila; D009479:Neuropeptides; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C093565:amn protein, Drosophila; C032175:benzaldehyde