Cold sensitivity of thermophilic and mesophilic RNA polymerases.
RNA polymerase from mesophilic Deinococcus radiodurans displays the same cold sensitivity of promoter opening as RNA polymerase from the closely related thermophilic Thermus aquaticus. This suggests that, contrary to the accepted view, cold sensitivity of promoter opening by thermophilic RNA polymerases may not be a consequence of their thermostability.
Kulbachinskiy
A
A
Institute of Molecular Genetics, Moscow, Russia. akulb@img.ras.ru
Bass
I
I
Bogdanova
E
E
Goldfarb
A
A
Nikiforov
V
V
D003080:Cold Temperature; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D034301:Deinococcus / Q000201:enzymology*; D004795:Enzyme Stability; D006358:Hot Temperature; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000378:metabolism; D013824:Thermus / Q000201:enzymology*; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases