title: Mycoplasma haemofelis and Mycoplasma haemominutum detection by polymerase chain reaction in cats from Saskatchewan and Alberta.
abstract: Hemobartonellosis is caused by Mycoplasma haemofelis, previously known as Haemobartonella felis. Cats infected with this organism typically develop regenerative anemia. The related species Mycoplasma haemominutum may also cause anemia. The purposes of this study were to use polymerase chain reaction technology to determine if both organisms exist in naturally infected cats from Saskatchewan and Alberta, and to determine if disease manifestation corresponds to mycoplasma species. Thirteen of 18 cats with regenerative anemia were infected, 12 with M. haemofelis and 1 with M. haemominutum. Eight of 22 cats with nonregenerative anemia were infected, 4 with M. haemofelis and 4 with M. haemominutum. Two of 20 cats with normal complete blood (cell) counts were infected with M. haemominutum. Although both mycoplasma species were identified, ill cats were more often infected with M. haemofelis.
authors.affiliation: Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Kewish
authors.forename: Greg D
authors.initials: GD
authors.lastname: Appleyard
authors.forename: Sherry L
authors.initials: SL
authors.lastname: Myers
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Kidney
authors.forename: Marion L
authors.initials: ML
authors.lastname: Jackson
mesh_terms: D000416:Alberta / Q000453:epidemiology; D000740:Anemia / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000453:epidemiology; D002415:Cats; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015995:Prevalence; D012525:Saskatchewan / Q000453:epidemiology; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial