title: Overexpression of the Wilms' tumor gene W T1 in primary astrocytic tumors.
abstract: Expression of the Wilms' tumor gene W T1 in primary astrocytic tumors was examined using a quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or immunohistochemistry. Real-time RT-PCR showed that W T1 mRNA was expressed at various levels in all of the 25 astrocytic tumors examined. Immunohistochemical analysis showed that W T1 protein was expressed in 5 of 6 low-grade astrocytic tumors (grade I-II) and all of 18 high-grade ones (grade III-IV), and that expression levels of W T1 protein in high-grade tumors were significantly higher than those in low-grade ones. W T1 protein was not detected in the normal glial cells contained in the tumor specimens. Furthermore, treatment with W T1 antisense oligomers specifically inhibited growth of glioblastoma cell lines, U87-MG, A172, and T-98G. These results may indicate that the W T1 gene plays an important role in tumorigenesis of primary astrocytic tumors.
authors.affiliation: Department of Bioinformatics, Osaka University Graduate School of Medicine, 1-7 Yamada-Oka, Suita, Osaka 565-0871, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oji
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakano
authors.forename: Motohiko
authors.initials: M
authors.lastname: Maruno
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Nakatsuka
authors.forename: Tanyarat
authors.initials: T
authors.lastname: Jomgeow
authors.forename: Sakie
authors.initials: S
authors.lastname: Abeno
authors.forename: Naoya
authors.initials: N
authors.lastname: Tatsumi
authors.forename: Asumi
authors.initials: A
authors.lastname: Yokota
authors.forename: Sayaka
authors.initials: S
authors.lastname: Aoyagi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Ken
authors.initials: K
authors.lastname: Ito
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kanato
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shirakata
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Nishida
authors.forename: Naoki
authors.initials: N
authors.lastname: Hosen
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawakami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tsuboi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oka
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yoshimine
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000293:Adolescent; D000368:Aged; D001254:Astrocytoma / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D025721:WT1 Proteins