title: [Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus].
abstract: OBJECTIVE
  To modify the structure of dehydroepiandrosterone (DHEA).
  
  
  METHODS
  Using hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.
  
  
  RESULTS
  (1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3, 17-dione (I); 6alpha-hydroxyandrost-4-ene-3, 17-dione (II); 6alpha, 17beta-dihydroxyandrost-4-ene-3-one (III); androst-4-ene-3, 6, 17-trione (IV) and 17beta-hydroxyandrost-4-ene-3-one (V).
  
  
  CONCLUSIONS
  It is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Ke-di
authors.initials: KD
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Feng
authors.forename: Chao
authors.initials: C
authors.lastname: Meng
authors.forename: Hui-xin
authors.initials: HX
authors.lastname: Zhu
authors.forename: Hui-xia
authors.initials: HX
authors.lastname: He
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Ma
mesh_terms: D000735:Androstenedione / Q000737:chemistry / Q000302:isolation & purification*; D000736:Androstenes / Q000737:chemistry / Q000302:isolation & purification*; D001711:Biotransformation; D003470:Culture Media; D003687:Dehydroepiandrosterone / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D018517:Plant Roots / Q000378:metabolism; D010946:Plants, Medicinal / Q000378:metabolism*; D019657:Solanaceae / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D000736:Androstenes; D003470:Culture Media; D000735:Androstenedione; D003687:Dehydroepiandrosterone; C018213:androst-4-ene-3,6,17-trione