title: A novel method of identifying genetic mutations using an electrochemical DNA array.
abstract: We describe the development of a new type of DNA array chip that utilizes electrochemical reactions and a novel method of simultaneously identifying multiple genetic mutations on an array chip. The electrochemical array (ECA) uses a threading intercalator specific to double-stranded nucleotides, ferrocenylnaphthalene diimide (FND), as the indicator. ECA does not require target labeling, and the equipment is simple, durable and less expensive. The simultaneous multiple mutation detection (SMMD) system using an ECA chip and FND utilizes an enzyme to simultaneously distinguish several genetic mutations such as single nucleotide polymorphism (SNP), insertion, deletion, translocation and short tandem repeat. We examined this SMMD system using an ECA chip, by detecting seven different mutations on the lipoprotein lipase (LPL) gene for 50 patients in a blind test. It turned out that all the results obtained were concordant with the sequencing results, demonstrating that this system is a powerful tool for clinical applications.
authors.affiliation: TUM Gene, Inc., 3-1 Kazusa-Koito Kimitsu, Chiba 292-1149 Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Wakai
authors.forename: Atsuko
authors.initials: A
authors.lastname: Takagi
authors.forename: Masato
authors.initials: M
authors.lastname: Nakayama
authors.forename: Takahito
authors.initials: T
authors.lastname: Miya
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Miyahara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Iwanaga
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Masahiko
authors.initials: M
authors.lastname: Amano
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Urata
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry; D006801:Humans; D007094:Imides / Q000737:chemistry; D008071:Lipoprotein Lipase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D005296:Ferrous Compounds; D007094:Imides; C406363:ferrocenylnaphthalene diimide; D008071:Lipoprotein Lipase