Sp1 is involved in the transcriptional activation of lysozyme in epithelial cells.
Lysozyme protects us from the ever-present danger of bacterial infection. The expression of lysozyme is, in part, regulated by the Ets factor, myeloid elf-1-like factor (MEF). MEF binds to the ETS site of the lysozyme promoter at -46 to -40bp. Closer analysis of the promoter using a series of deletion mutants and point mutants indicated that the region around -75bp is also essential in regulating the activity of lysozyme. The sequences in this region correspond to the Sp1 consensus binding site. Sp1 is known to regulate a variety of house-keeping and tissue-specific genes by itself or with other transcription factors like AP-1 or ETS. We indicate here that Sp1 regulates the lysozyme gene by binding to the GT-core sequences of lysozyme promoter. Treatment with mithramycin A down-regulated the promoter activity and the transfection of anti-sense Sp1 induced a decrease in the endogenous expression of lysozyme.
Suico
Mary Ann
MA
Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
Koga
Tomoaki
T
Shuto
Tsuyoshi
T
Hisatsune
Akinori
A
Lu
Zhuo
Z
Basbaum
Carol
C
Okiyoneda
Tsukasa
T
Kai
Hirofumi
H
D024506:5' Flanking Region; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000201:enzymology; D006801:Humans; D009113:Muramidase / Q000096:biosynthesis / Q000235:genetics*; D008926:Plicamycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C066851:mithramycin A; D009113:Muramidase; D008926:Plicamycin