title: Lymphocyte activation receptors: new structural paradigms in group V of C-type animal lectins.
abstract: The structure-function relationship in group V of C-type animal lectins remains incompletely understood despite the new structures of NK (natural killer) cell receptors that have been solved recently. Recombinant, soluble forms of rat and human NKR-P1 and CD69 that we obtained after in vitro refolding were analysed by Fourier transform-ion cyclotron resonance MS and heteronuclear NMR ((1)H-(15)N correlation). In NKR-P1, calcium may not be removed by chelating agents because of the very high affinity of binding. In CD69, incorporation of calcium causes a structural shift in several amino acids important for the interaction with carbohydrates. Structural studies have also allowed us to understand an interesting preference of these receptors for either linear (NKR-P1) or branched (CD69) carbohydrate sequences.
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic. pavlicek@biomed.cas.cz
authors.forename: J
authors.initials: J
authors.lastname: Pavlícek
authors.forename: D
authors.initials: D
authors.lastname: Kavan
authors.forename: P
authors.initials: P
authors.lastname: Pompach
authors.forename: P
authors.initials: P
authors.lastname: Novák
authors.forename: O
authors.initials: O
authors.lastname: Luksan
authors.forename: K
authors.initials: K
authors.lastname: Bezouska
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D037181:Lectins, C-Type / Q000737:chemistry / Q000502:physiology*; D008213:Lymphocyte Activation / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D051381:Rats; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D000099192:CD69 Antigens