Determination of proteins with fullerol by a resonance light scattering technique.
Fullerol has been synthesized through the reaction of fullerene C60 with NaOH in aqueous solution by means of ultrasonic agitation and characterized by infrared and 1H-nuclear magnetic resonance spectroscopy. The fullerol obtained shows good solubility and excellent stability in water. A weak resonance light scattering (RLS) spectrum of fullerol was observed in aqueous solution. However, the intensity of the RLS signal could be enhanced in the presence of proteins, including bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep), and lysozyme (Lys). Based on the enhancement of the RLS, a sensitive method for the determination of proteins has been established. The quantitative conditions were considered with regard to the effects of the pH, the ion strength, and the concentration of the fullerol. Under the optimum conditions, the intensity of the RLS was proportional to the concentration of proteins with the limits of detection of 9.7, 10.9, 57.4, and 8.5 ng mL(-1) for BSA, HSA, Pep, and Lys, respectively. Almost no interference can be observed from some amino acids, nucleic acids, and most of the metal ions. The model samples and human serum samples were determined satisfactorily with the proposed method.
Zhao
Guang-Chao
GC
School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China. gczhao@mail.ahnu.edu.cn
Zhang
Ping
P
Wei
Xian-Wen
XW
Yang
Zhou-Sheng
ZS
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002138:Calibration; D002417:Cattle; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D009994:Osmolar Concentration; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D012542:Scattering, Radiation; D013997:Time Factors
D001798:Blood Proteins; D037741:Fullerenes; D011506:Proteins