title: Proteome analysis of focal adhesion kinase (FAK)-overexpressing cells.
abstract: We established several focal adhesion kinase (FAK) cDNA-transfected cells and found that FAK-transfected HL-60 (HL-60/FAK) cells are resistant to apoptosis induced with hydrogen peroxide, etoposide and radiation compared with the parental HL-60 or the vector-transfected (HL-60/Vect) cells. We carried out proteome analysis to study the mechanism of resistance to apoptosis in HL-60/FAK cells. Among 300 spots resolved in two-dimensional gels, ca. 10% of them were significantly increased in HL-60/FAK cells compared with HL-60/Vect cells, whereas ca. 2% of them were decreased or disappeared. These proteins were performed for further analysis by Western blots or N-terminal sequencing or mass spectrometry. Increased proteins included stress proteins such as hsp90, ribosomal proteins, and antioxidant enzymes such as peroxyredoxin 2. Some of these proteins are assumed to contribute to the antiapoptotic action of FAK.
authors.affiliation: Department of Biochemistry, Kyoritsu University of Pharmacy, Tokyo, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Utsubo
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sonoda
authors.forename: Reiko
authors.initials: R
authors.lastname: Takahashi
authors.forename: Shiro
authors.initials: S
authors.lastname: Iijima
authors.forename: Eriko
authors.initials: E
authors.lastname: Aizu-Yokota
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kasahara
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D013058:Mass Spectrometry; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D020543:Proteome / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013879:Thioredoxins / Q000096:biosynthesis; D014162:Transfection
chemical_list: D020543:Proteome; D012333:RNA, Messenger; D013879:Thioredoxins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human