Survival and injury of Arcobacter after artificial inoculation into drinking water.
The aim of this work was to assess the effect of chlorine water treatment on Arcobacter butzleri and to study the survival strategies of this organism in chlorinated and non-chlorinated drinking water. A. butzleri NCTC 12481 was inoculated into chlorinated and non-chlorinated water and samples were removed aseptically, immediately and periodically during the next 2 days (for chlorinated drinking water) or 35 days (for non-chlorinated drinking water). The membrane integrity (Live/Dead BacLight kit), 16S rRNA (FISH technique), DNA content (23S rRNA PCR-RFLPs) and culturability changes in A. butzleri cells were analyzed. Culturability of the cells was lost at 5 min in chlorinated drinking water. At that time the cells showed membrane damage, although fluorescent intensity of 16S rRNA hybridization was constant throughout the chlorine treatment. After 48 h the amplicon specific for the 23S rRNA gene was weakly detected. In non-chlorinated drinking water cells lost their culturability after 16 days but the other factors measured indicated that Arcobacter remained viable throughout the experiment.
Moreno
Yolanda
Y
Departamento de Biotecnología, Universidad Politécnica, Camino de Vera 14, 46022 Valencia, Spain.
Alonso
José Luis
JL
Botella
Salut
S
Ferrús
M Antonia
MA
Hernández
Javier
J
D020640:Arcobacter / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002713:Chlorine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004275:DNA, Ribosomal / Q000032:analysis; D004203:Disinfection / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D014881:Water Supply
D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D002713:Chlorine