Influence of amphotericin B on the ciliary beat frequency of nasal mucosa.
OBJECTIVE
A new therapeutic approach in eosinophilic fungal rhinosinusitis is topical application of antifungals such as amphotericin B. The tolerability of a recently applied concentration of this drug by human nasal mucosa was tested in vitro.


METHODS
Ciliary beat frequency (CBF) was measured by a photometric technique, combining a light microscope, a photometer, a photograph-multiplier, and a computed analyzing unit.


RESULTS
Treatment for 20 minutes with amphotericin B finally diluted in saline to 0.1 mg/mL caused no decrease in CBF. By contrast, both amphotericin B diluted in distilled water and distilled water without additives lowered CBF about 50%. This decrease was not reversible after reincubation for 20 minute in saline.


CONCLUSIONS
The results confirm the good tolerability of topical amphotericin B in the nasal and paranasal cavity. Because of the negative effect of distilled water, final dilution in physiologic solvents should be considered.
Hofer
Edmund
E
Department of Otorhinolaryngology, University Hospital of Innsbruck, Innsbruck, Austria.
Neher
Andreas
A
Schrott-Fischer
Anneliese
A
Nagl
Markus
M
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009068:Movement / Q000187:drug effects / Q000502:physiology; D009297:Nasal Mucosa / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
D000935:Antifungal Agents; D000666:Amphotericin B