title: CDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae).
abstract: We cloned the Bombina orientalis adenosine triphosphate (ATP) synthase subunit b gene from a B. orientalis oviduct cDNA library. The transcript was 997 bp long and encoded 250 amino acid residues. It showed high similarity to amphibian (84-85%), mammalian (56-62%) and invertebrate (46-50%) sequences. In phylogenetic analyses, it clustered with other amphibian sequences. This gene was highly expressed in brain, intestine and oviduct but not in muscle and liver. In this paper, we report the basic characteristics of B. orientalis ATP synthase subunit b gene.
authors.affiliation: Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Sook-Kyoung
authors.initials: SK
authors.lastname: Kim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Han Seung
authors.initials: HS
authors.lastname: Kang
authors.forename: Hee-Seok
authors.initials: HS
authors.lastname: Kweon
authors.forename: Ui-Wook
authors.initials: UW
authors.lastname: Hwang
authors.forename: Moon Kyoo
authors.initials: MK
authors.lastname: Kim
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.forename: Yong-Dal
authors.initials: YD
authors.lastname: Yoon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010057:Oviducts / Q000378:metabolism*; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D006180:Proton-Translocating ATPases