title: Ageing affects the differentiation potential of human myoblasts.
abstract: The ageing process causes a reduction in the regenerative potential of skeletal muscles eventually leading to diminished muscle strength. In this work we investigated if ageing affects the excitation-contraction coupling mechanism in human myotubes derived from human satellite cells, thereby contributing to the loss in muscle strength in the aged. To test this hypothesis, satellite cells from differently aged donors were differentiated in vitro and the maturation of the excitation-contraction mechanism was followed by the videoimaging technique monitoring the efficiency of such a mechanism in generating intracellular calcium transients. Our experiments showed a delay in the establishment of the excitation-contraction coupling mechanism depending on the age of the donor. Remarkably, the effect was reproducible in human satellite cells from a young donor aged in vitro, suggesting that the delayed functional maturation was strictly dependent on the number of satellite cell divisions and independent from the host environment.
authors.affiliation: Department of Physiology and Pathology and Centre for Neuroscience B.R.A.I.N., University of Trieste, via A. Fleming 22, I-34127 Trieste, Italy. pielle@dfpmail.units.it
authors.forename: Paola
authors.initials: P
authors.lastname: Lorenzon
authors.forename: Elena
authors.initials: E
authors.lastname: Bandi
authors.forename: Francesca
authors.initials: F
authors.lastname: de Guarrini
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pietrangelo
authors.forename: Ralf
authors.initials: R
authors.lastname: Schäfer
authors.forename: Margit
authors.initials: M
authors.lastname: Zweyer
authors.forename: Anton
authors.initials: A
authors.lastname: Wernig
authors.forename: Fabio
authors.initials: F
authors.lastname: Ruzzier
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002459:Cell Fusion; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D002675:Child, Preschool; D003893:Desmin / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009119:Muscle Contraction / Q000502:physiology; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
chemical_list: D015220:Calcium Channels; D003893:Desmin