title: Community-onset disease caused by Citrobacter freundii producing a novel CTX-M beta-lactamase, CTX-M-30, in Canada.
abstract: Strains of Citrobacter freundii intermediate to cefotaxime but sensitive to ceftazidime were isolated from four different patients in Canada. Sequencing of PCR products by use of CTX-M-specific primers revealed a new combination of four amino acid substitutions. This new gene was designated bla(CTX-M-30) and was encoded on a 3-kb plasmid. The pI of CTX-M-30 was 8.0.
authors.affiliation: Center for Research in Antiinfectives & Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.
authors.forename: Baha
authors.initials: B
authors.lastname: Abdalhamid
authors.forename: Johann D D
authors.initials: JD
authors.lastname: Pitout
authors.forename: Ellen S
authors.initials: ES
authors.lastname: Moland
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Hanson
mesh_terms: D015139:Blotting, Southern; D002170:Canada; D016971:Citrobacter freundii / Q000187:drug effects* / Q000201:enzymology; D017714:Community-Acquired Infections / Q000382:microbiology* / Q000652:urine; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology* / Q000652:urine; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007525:Isoelectric Focusing; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases; C494144:beta-lactamase CTX-M-30, Citrobacter freundii