Global position and recruitment of HATs and HDACs in the yeast genome.
Chromatin regulators play fundamental roles in the regulation of gene expression and chromosome maintenance, but the regions of the genome where most of these regulators function has not been established. We explored the genome-wide occupancy of four different chromatin regulators encoded in Saccharomyces cerevisiae. The results reveal that the histone acetyltransferases Gcn5 and Esa1 are both generally recruited to the promoters of active protein-coding genes. In contrast, the histone deacetylases Hst1 and Rpd3 are recruited to specific sets of genes associated with distinct cellular functions. Our results provide new insights into the association of histone acetyltransferases and histone deacetylases with the yeast genome, and together with previous studies, suggest how these chromatin regulators are recruited to specific regions of the genome.
Robert
François
F
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA. francois.robert@ircm.qc.ca
Pokholok
Dmitry K
DK
Hannett
Nancy M
NM
Rinaldi
Nicola J
NJ
Chandy
Mark
M
Rolfe
Alex
A
Workman
Jerry L
JL
Gifford
David K
DK
Young
Richard A
RA
D000123:Acetyltransferases / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism; D016678:Genome; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000378:metabolism*; D009243:NAD / Q000096:biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D056565:Sirtuin 2; D037761:Sirtuins / Q000378:metabolism; D013172:Spores, Fungal / Q000201:enzymology / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D004268:DNA-Binding Proteins; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases; D009243:NAD; D011494:Protein Kinases; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D056565:Sirtuin 2; D037761:Sirtuins; D014157:Transcription Factors; D014364:Tryptophan; C496551:Esa1 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C103934:HST1 protein, S cerevisiae; C071665:RPD3 protein, S cerevisiae