MicroRNA-143 regulates adipocyte differentiation.
MicroRNAs (miRNAs) are endogenously expressed 20-24 nucleotide RNAs thought to repress protein translation through binding to a target mRNA (1-3). Only a few of the more than 250 predicted human miRNAs have been assigned any biological function. In an effort to uncover miRNAs important during adipocyte differentiation, antisense oligonucleotides (ASOs) targeting 86 human miRNAs were transfected into cultured human pre-adipocytes, and their ability to modulate adipocyte differentiation was evaluated. Expression of 254 miRNAs in differentiating adipocytes was also examined on a miRNA microarray. Here we report that the combination of expression data and functional assay results identified a role for miR-143 in adipocyte differentiation. miR-143 levels increased in differentiating adipocytes, and inhibition of miR-143 effectively inhibited adipocyte differentiation. In addition, protein levels of the proposed miR-143 target ERK5 (4) were higher in ASO-treated adipocytes. These results demonstrate that miR-143 is involved in adipocyte differentiation and may act through target gene ERK5.
Esau
Christine
C
Isis Pharmaceuticals, Carlsbad, California 92008, USA. cesau@isisph.com
Kang
Xiaolin
X
Peralta
Eigen
E
Hanson
Elaine
E
Marcusson
Eric G
EG
Ravichandran
Lingamanaidu V
LV
Sun
Yingqing
Y
Koo
Seongjoon
S
Perera
Ranjan J
RJ
Jain
Ravi
R
Dean
Nicholas M
NM
Freier
Susan M
SM
Bennett
C Frank
CF
Lollo
Bridget
B
Griffey
Richard
R
D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
D015342:DNA Probes; D035683:MicroRNAs; D020319:Oligodeoxyribonucleotides, Antisense