title: On the role of lipid in colicin pore formation.
abstract: Insights into the protein-membrane interactions by which the C-terminal pore-forming domain of colicins inserts into membranes and forms voltage-gated channels, and the nature of the colicin channel, are provided by data on: (i) the flexible helix-elongated state of the colicin pore-forming domain in the fluid anionic membrane interfacial layer, the optimum anionic surface charge for channel formation, and voltage-gated translocation of charged regions of the colicin domain across the membrane; (ii) structure-function data on the voltage-gated K(+) channel showing translocation of an arginine-rich helical segment through the membrane; (iii) toroidal channels formed by small peptides that involve local participation of anionic lipids in an inverted phase. It is proposed that translocation of the colicin across the membrane occurs through minimization of the Born charging energy for translocation of positively charged basic residues across the lipid bilayer by neutralization with anionic lipid head groups. The resulting pore structure may consist of somewhat short, ca. 16 residues, trans-membrane helices, in a locally thinned membrane, together with surface elements of inverted phase lipid micelles.
authors.affiliation: Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Zakharov
authors.forename: Elena A
authors.initials: EA
authors.lastname: Kotova
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Antonenko
authors.forename: William A
authors.initials: WA
authors.lastname: Cramer
mesh_terms: D003087:Colicins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008563:Membrane Lipids / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry; D008823:Micelles; D011487:Protein Conformation; D055672:Static Electricity
chemical_list: D003087:Colicins; D008563:Membrane Lipids; D008565:Membrane Proteins; D008823:Micelles