[Neoplastic transformation of mouse fibroblasts under the influence of high-energy protons and gamma-rays].
Oncoginic transformations of mouse fibroblasts C3H10T1/2 after exposure to proton energies 150 and 584 MeV were compared with fibroblast effects of gamma-radiation. Prior to exposure, cell populations (2.7 x 10(3) cells/cm2) were inoculated in plastic vials with the surface area of 75 cm2 and cultivated 11 days. Survivability was determined by comparing the number of cell colonies in irradiated and non-irradiated (control) vials. Transformation rate was calculated by dividing the total transformation focus number by the number of survived cells in a vial. Rate of oncogenic transformations after gamma- and proton (584 MeV) irradiation was essentially identical, i.e. the parameter grew rapidly at the doses < 1 Gy and slowed down at the doses > 1 Gy. In the dose interval between 1 and 5 Gy, transformation rate for proton energy 150 MeV was found low compared with gamma-radiation and proton energy 584 MeV. It is hypothesized that the different transformation rate after exposure to proton energy 150 MeV is linked with the high linear energy transfer as compared with the proton energy of 584 MeV and gamma-radiation.
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D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology* / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D051379:Mice; D009678:Nuclear Energy; D011522:Protons / Q000009:adverse effects*; D011829:Radiation Dosage
D011522:Protons