title: Expressed sequence tag (EST) analysis of Gregarine gametocyst development.
abstract: Gregarines are protozoan parasites of invertebrates in the phylum Apicomplexa. We employed an expressed sequence tag strategy in order to dissect the molecular processes of sexual or gametocyst development of gregarines. Expressed sequence tags provide a rapid way to identify genes, particularly in organisms for which we have very little molecular information. Analysis of approximately 1800 expressed sequence tags from the gametocyst stage revealed highly expressed genes related to cell division and differentiation. Evidence was found for the role of degradation and recycling in gametocyst development. Numerous additional genes uncovered by expressed sequence tag sequencing should provide valuable tools to investigate gametocyst development as well as for molecular phylogenetics, and comparative genomics in this important group of parasites.
authors.affiliation: School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA. omoto@wsu.edu
authors.forename: Charlotte K
authors.initials: CK
authors.lastname: Omoto
authors.forename: Marc
authors.initials: M
authors.lastname: Toso
authors.forename: Keliang
authors.initials: K
authors.lastname: Tang
authors.forename: L David
authors.initials: LD
authors.lastname: Sibley
mesh_terms: D000818:Animals; D016782:Apicomplexa / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D020451:Contig Mapping / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D020224:Expressed Sequence Tags; D015870:Gene Expression / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics*; D008018:Life Cycle Stages / Q000235:genetics; D008969:Molecular Sequence Data; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D016054:DNA, Protozoan