title: PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-specific primer pair.
abstract: A PCR-based assay to identify pandemic group Vibrio parahaemolyticus has been developed. The assay employs an oligonucleotide primer pair derived from the group-specific sequence of an arbitrarily primed-PCR fragment, which is located in the genome encoding a "hypothetical protein," approximately 80% homologous to the Mn2+ and Fe2+ transporter of the NRAMP family of V. vulnificus. The assay distinguished the pandemic group from other V. parahaemolyticus strains by yielding a 235-bp specific amplicon, and can be a useful diagnostic tool for identification of pandemic group strains.
authors.affiliation: Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okura
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iguchi
authors.forename: Michihiro
authors.initials: M
authors.lastname: Takagi
authors.forename: Eiji
authors.initials: E
authors.lastname: Arakawa
authors.forename: Jun
authors.initials: J
authors.lastname: Terajima
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D027682:Cation Transport Proteins; D017931:DNA Primers; D004196:Disease Outbreaks; D014735:Vibrio Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers