title: RNA encoding the MPT83 antigen induces protective immune responses against Mycobacterium tuberculosis infection.
abstract: We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies.
authors.affiliation: Mycobacterial Division, National Institute for Medical Research, London NW7 1AA, UK.
authors.forename: Tian
authors.initials: T
authors.lastname: Xue
authors.forename: Evangelos
authors.initials: E
authors.lastname: Stavropoulos
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Silvia
authors.initials: S
authors.lastname: Ragno
authors.forename: Martin
authors.initials: M
authors.lastname: Vordermeier
authors.forename: Mark
authors.initials: M
authors.lastname: Chambers
authors.forename: Glyn
authors.initials: G
authors.lastname: Hewinson
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Lowrie
authors.forename: M Joseph
authors.initials: MJ
authors.lastname: Colston
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Tascon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D012329:RNA, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012845:Sindbis Virus / Q000201:enzymology / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D012324:RNA-Dependent RNA Polymerase