[Effect of adenovirally delivered brain-derived neurotrophic factor on superoxide dismutase within the retina of experimentally injured optic nerve of rat eyes].
OBJECTIVE
To study the changes of malondialdehyde(MDA) and superoxide dismutase (SOD) in the retinal tissues of the rats after optic nerve injury and the influence to it by adenovirally delivered brain-derived neurotrophic factor (BDNF) to ocular tissues.


METHODS
Adenovirus with BDNF gene, adenovirus with beta-galactosidase gene and balanced salt solution were injected, respectively, into vitreous of injured eyes of rat. The content of MDA and SOD in the retinal tissues of the rats were measured by means of quantity analysis in various time. That of normal retinal tissues were examined and used as control.


RESULTS
The content of MDA and SOD in the normal retina was (5.33 +/- 1.45) nmol/ ml (21d), fewer than the two control groups' and the contents of SOD of BDNF group were (1587.55 +/- 173.39) U/g x pro (3d), (1613.24 +/- 184.57) U/g x pro(7d) (1694. 49 +/- 184. 30) U/g x pro (14d) and (1721.75 +/- 179.86) U/g x pro(21d), more than the two control groups' meanwhile. There were significent differences at 3d, 1 wk and 2 wk (P < 0.05). There were no significent differences between the two control groups at every time point.


CONCLUSIONS
Adenovirally delivered BDNF to murine ocular tissues after optic nerve injury helps protect from oxidative stress during earlier period. The effect may be related to the increased amount of SOD within local tissue by BDNF.
Hu
Dan
D
Department of Ophthalmology, in Hospital, the Fourth Military Medical University, Xi' an 710032, China.
Hou
Xu
X
Hui
Yannian
Y
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism*; D020221:Optic Nerve Injuries / Q000188:drug therapy / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014162:Transfection
D019208:Brain-Derived Neurotrophic Factor; D008315:Malondialdehyde; D013482:Superoxide Dismutase