Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis.
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as anti-Leishmania total antigen antibodies. When cross-reactivity was tested, chronic Chagas' disease patients (N = 10) did not show any reactivity. Therefore, we consider this L. infantum Hsp 83 to be a good antigen for routine use for serodiagnosis of tegumentary leishmaniasis.
Celeste
B J
BJ
Laboratório de Soroepidemiologia e Imunobiologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brasil.
Angel
S O
SO
Castro
L G M
LG
Gidlund
M
M
Goto
H
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D014355:Chagas Disease / Q000175:diagnosis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins; D006801:Humans; D018314:Leishmania infantum / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D015800:Protozoan Proteins; D012698:Serologic Tests / Q000379:methods
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D006360:Heat-Shock Proteins; C490310:Hsp83 protein, protozoan; D015800:Protozoan Proteins