title: Cloning and expression analysis of a bZIP cDNA in Gossypium hirsutum L.
abstract: A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L. The cDNA, designated GhbZIP,encoded a polypeptide of 645 amino acids. GhbZIP protein had the structure characteristics of plant bZIP proteins, including two conserved domains, DUF630 and DUF632 with unknown functions, a proline-rich domain and a phenylalanine-rich domain. Meanwhile, the protein contained a leucine zipper-like motif in DUF632. The hydropathy analysis showed that the GhbZIP was a membrane protein. GhbZIP gene was preferentially expressed in ovule and fiber cells since three days post-anthesis, as indicated it might be involved in the transcription regulation of genes during cotton fiber elongation.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jian-Xiong
authors.initials: JX
authors.lastname: Jiang
authors.forename: Wang-Zhen
authors.initials: WZ
authors.lastname: Guo
authors.forename: Tian-Zhen
authors.initials: TZ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D051790:G-Box Binding Factors; D020869:Gene Expression Profiling; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D014157:Transcription Factors