title: High efficiency DNA mutagenesis mediated by using in vitro transcription, DNase I digestion, and RT-PCR.
authors.affiliation: Chinese Academy of Sciences, Beijing, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xin
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
mesh_terms: D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase; D003850:Deoxyribonuclease I / Q000378:metabolism*; D066298:In Vitro Techniques; D016297:Mutagenesis, Site-Directed; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase; D003850:Deoxyribonuclease I