High efficiency DNA mutagenesis mediated by using in vitro transcription, DNase I digestion, and RT-PCR.
Xin
Wen
W
Chinese Academy of Sciences, Beijing, P.R. China.
Huang
Da-Wei
DW
Xiao
Hui
H
D004247:DNA / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase; D003850:Deoxyribonuclease I / Q000378:metabolism*; D066298:In Vitro Techniques; D016297:Mutagenesis, Site-Directed; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase; D003850:Deoxyribonuclease I