Expression of the peripheral benzodiazepine receptor is decreased in skin cancers in comparison with normal skin.
BACKGROUND
The peripheral benzodiazepine receptor (PBR) is an 18-kDa protein receptor mainly found on the outer mitochondrial membrane of cells. The PBR plays a role in several cellular functions including haem synthesis, steroidogenesis, DNA synthesis, cell growth and differentiation, and apoptosis. PBR expression in normal skin correlates with proliferating, secretory and differentiated cellular structures. Increased or aberrant expression of PBR has been associated with aggressive behaviour in several tumour types including ovarian, colon and breast adenocarcinomas and glioblastoma.


OBJECTIVE
To determine whether changes in normal PBR distribution would be useful as markers for skin cancers or possible target sites for therapies such as photodynamic therapy (PDT), we used immunohistochemistry to evaluate PBR expression and distribution in normal and photodamaged skin (actinic keratoses), skin cancers (in situ and invasive squamous cell carcinomas and superficial, nodular, morphoeiform and mixed pattern basal cell carcinomas) and several benign epithelial proliferations.


METHODS
A rabbit polyclonal antibody to a synthetic peptide fragment of the PBR was developed and characterized by enzyme-linked immunosorbent assay and Western blot analysis. The antibody was used to stain formalin-fixed and paraffin-embedded tissue samples (n = 157) by a routine avidin-biotin immunohistochemical technique. Sections were evaluated for antibody localization, distribution (0-4+) and reaction intensity (negative to strong).


RESULTS
Normal skin stained with a strong homogeneous positive reaction (3-4+) in the spinous and granular layers (with a gradient corresponding to increasing differentiation), the pilosebaceous units, eccrine gland ducts, endothelial cells and pilar muscle. In cutaneous neoplasms and other skin diseases, a heterogeneous pattern (0-4+) of PBR expression at lower intensity was seen depending on tumour type and degree of differentiation. PBR expression was greatest in well-differentiated tumours, synonymous with the PBR expression gradient seen in normal skin; and least in poorly differentiated and infiltrative tumour types.


CONCLUSIONS
The haem biosynthetic pathway has been harnessed for PDT of skin carcinomas by application of exogenous aminolaevulinic acid to generate the endogenous photosensitizer protoporphyrin IX (PpIX). Owing to the role of PBR as a transporter of haem precursors in haem synthesis, PBR density and distribution in skin cancers could be a predictor of the capacity for PpIX production and subsequent response to PDT in skin cancers.
Morgan
J
J
Department of Dermatology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. Janet.Morgan@Roswell.Park.org
Oseroff
A R
AR
Cheney
R T
RT
D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D002280:Basal Cell Carcinoma / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D006801:Humans; D007642:Keratosis / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D010778:Photochemotherapy; D010787:Photosensitivity Disorders / Q000378:metabolism; D011230:Precancerous Conditions / Q000378:metabolism; D011379:Prognosis; D011963:Receptors, GABA-A / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D012871:Skin Diseases / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011963:Receptors, GABA-A