Description of an ultrathin multiwire proportional chamber-based detector and application to the characterization of the Spraguea lophii (Microspora) two-dimensional genome fingerprint.
Multiwire proportional chamber is a useful technology to build detectors that supersede the lack of interactivity of autoradiography in molecular biology experiments. Some drawbacks still limited the diffusion of existing instruments in biological laboratories. The major competitors are storage phosphor imaging systems. The simplified description of a radio-chromato-imager prototype (RCI) based on an original ultrathin multiwire proportional chamber is presented. It combines the advantage of the different existing technologies to present competitive properties in terms of efficiency, spatial resolution, robustness, manipulation easiness and production cost. Application of the RCI detector to molecular biology was performed by the analysis of karyotype and restriction display two-dimensional pulsed-field gel electrophoresis (KARD 2-D PFGE) data which are used to describe small eukaryotic genome structures. The comparative analysis with autoradiography was performed with the PDQuest software on Spraguea lophii (Microspora) genome fingerprints. The spot detection procedure applied to the different images leads to a similar conclusion considering the genome structure of S. lophii which appeared to be composed of 15 chromosomes for 13 karyotypic bands (200-880 kbp).
Mansour
Lamjed
L
Parasitologie Moléculaireet Cellulaire, Université Blaise Pascal, Aubière, France. emmanuel.cornillot@univ-bpclermont.fr
Cheikali
Chafik
C
Desaunais
Pierre
P
Coulon
Jean-Pierre
JP
Daubin
Josette
J
Hassine
Oum Kaltoum Ben
OK
Vivarès
Christian P
CP
Jeanjean
Jack
J
Cornillot
Emmanuel
E
D000818:Animals; D016817:Apansporoblastina / Q000737:chemistry / Q000235:genetics*; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D016054:DNA, Protozoan / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation*; D018503:Genome, Protozoan; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D007621:Karyotyping / Q000295:instrumentation / Q000379:methods
D016054:DNA, Protozoan