Identification of genes regulated by oleic acid in Jurkat cells by suppressive subtractive hybridization analysis.
In this study, the effect of oleic acid (50 microM) on gene expression of Jurkat cells (human T lymphocytes cell line) was examined using the suppressive subtractive hybridization approach. This technique allowed us to identify genes with higher or lower expression after cell treatment with oleic acid as compared to untreated cells. Oleic acid upregulated the expression of the translation elongation factor alpha 1 and ATP synthase 8 and downregulated gp96 (human tumor rejection antigen gp96), heat-shock protein 60 and subtilisin-like protein 4. These results suggest that oleic acid, at plasma physiological concentration, can regulate the expression of important genes to maintain the machinery that ensures cell functioning.
Martins de Lima
Thais
T
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Professor Lineu Prestes, 1524, Butantã, São Paulo, SP 05508-900, Brazil.
de Oliveira Rodrigues
Leonardo
L
Bengtson
Mario Henrique
MH
Sogayar
Mari Cleide
MC
Bezerra
Camila Nogueira Alves
CN
Rebouças
Nancy Amaral
NA
Curi
Rui
R
D015152:Blotting, Northern; D003001:Cloning, Molecular; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D019169:Jurkat Cells; D019301:Oleic Acid / Q000494:pharmacology*; D013601:T-Lymphocytes
D019301:Oleic Acid