title: Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis.
abstract: The enzymes of the shikimate pathway are potential targets for the development of new therapies because they are essential for bacteria but absent from mammals. The last step in this pathway is performed by chorismate synthase (CS), which catalyzes the conversion of 5-enolpyruvylshikimate-3-phosphate to chorismate. Optimization of crystallization trials allowed the crystallization of homogeneous recombinant CS from Mycobacterium tuberculosis (MtCS). The crystals of MtCS belong to space group P6(4)22 (or P6(2)22) and diffract to 2.8 A resolution, with unit-cell parameters a = b = 129.7, c = 156.8 A. There are two molecules in the asymmetric unit. Molecular-replacement trials were not successful. Heavy-atom derivative screening is in progress.
authors.affiliation: Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
authors.forename: Marcio Viniciusbertacine
authors.initials: MV
authors.lastname: Dias
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ely
authors.forename: Fernanda
authors.initials: F
authors.lastname: Canduri
authors.forename: José Henrique
authors.initials: JH
authors.lastname: Pereira
authors.forename: Jeverson
authors.initials: J
authors.lastname: Frazzon
authors.forename: Luiz Augusto
authors.initials: LA
authors.lastname: Basso
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Palma
authors.forename: Walter Filgueira
authors.initials: WF
authors.lastname: de Azevedo
authors.forename: Diógenes Santiago
authors.initials: DS
authors.lastname: Santos
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry*
chemical_list: C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases