title: Gene silencing in androgen-responsive prostate cancer cells from the tissue-specific prostate-specific antigen promoter.
abstract: The success of gene therapy using a RNA interference approach relies on small interfering RNA (siRNA) expression from a highly tissue-specific RNA polymerase II promoter rather than from ubiquitous RNA polymerase III. Accordingly, we have developed a prostate-specific vector that expresses siRNAs from the human prostate-specific antigen promoter, a RNA polymerase II promoter. Our data demonstrate androgen-dependent and tissue-specific siRNA-mediated gene silencing in the androgen-responsive prostate cancer cell line, LNCaP. The biological significance was evidenced by altered apoptotic activity through the inhibition of the apoptosis-related regulatory gene. These results demonstrate that siRNA-mediated gene silencing from a tissue-specific RNA polymerase II promoter could be a potential tool for tissue-specific gene therapy.
authors.affiliation: Dental Research Institute, University of California Los Angeles (UCLA) School of Dentistry, UCLA, Los Angeles, California 90095, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.forename: Shen
authors.initials: S
authors.lastname: Pang
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Lu
authors.forename: Kazunari K
authors.initials: KK
authors.lastname: Yokoyama
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Zheng
authors.forename: Robert
authors.initials: R
authors.lastname: Chiu
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D020287:In Situ Nick-End Labeling; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017430:Prostate-Specific Antigen / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D000728:Androgens; D034741:RNA, Small Interfering; D048031:JNK Mitogen-Activated Protein Kinases; D017430:Prostate-Specific Antigen