title: Protection against experimental Aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes.
abstract: Liposome-entrapped atypical Aeromonas salmonicida antigen was prepared to investigate the potential protective efficacy for A. salmonicida infection. Carp (Cyprinus carpio) were immunised orally with liposome-entrapped A. salmonicida antigen. After immunisation, significantly higher antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunised control group. Furthermore, immunised carp were challenged by immersion with 1 x 10(6) cfu ml(-1) of A. salmonicida for 60 min. Of the eight non-immunised carp, three carp died (62.5% survival), whereas five out of six (83.5%) immunised survived. Furthermore, the development of skin ulcers was significantly inhibited in carp immunised with liposomes containing A. salmonicida antigen. These results suggest that liposomes containing A. salmonicida antigen have the potential for the induction of a protective immune response against atypical A. salmonicida infection and also suggest the possibility of developing a vaccine that may ultimately be used for the prevention of fish diseases.
authors.affiliation: Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuen-cho, 1-1, Sakai, Osaka 599-8531, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Irie
authors.forename: Shinobu
authors.initials: S
authors.lastname: Watarai
authors.forename: Tadashi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kodama
mesh_terms: D000284:Administration, Oral; D048409:Aeromonas salmonicida / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage*; D002347:Carps; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D005667:Furunculosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007114:Immunization; D008081:Liposomes
chemical_list: D000942:Antigens, Bacterial; D008081:Liposomes