Vibrio neonatus sp. nov. and Vibrio ezurae sp. nov. isolated from the gut of Japanese abalones.
Five alginolytic, facultative anaerobic, non-motile bacteria were isolated from the gut of Japanese abalones (Haliotis discus discus, H. diversicolor diversicolor and H. diversicolor aquatilis). Phylogenetic analyses based on 16S rRNA gene and gap gene sequences indicated that these strains are closely related to V. halioticoli. DNA-DNA hybridizations, FAFLP fingerprintings, and phylogenies of gap and 16S rRNA gene sequences showed that the five strains represent two species different from all currently described vibrios. The names Vibrio neonatus sp. nov. (IAM 15060T = LMG 19973T = HDD3-1T; mol% G+C of DNA is 42.1-43.9), and Vibrio ezurae sp. nov. (IAM 15061T = LMG 19970T = HDS1-1T; mol% G+C of DNA is 43.6-44.8) are proposed to encompass these new taxa. The two new species can be differentiated from V. halioticoli on the basis of several features, including beta-galactosidase activity, assimilation of glycerol, D-mannose and D-gluconate.
Sawabe
Tomoo
T
Laboratory of Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan. sawabe@fish.hokudai.ac.jp
Hayashi
Karin
K
Moriwaki
Jun
J
Fukui
Youhei
Y
Thompson
Fabiano L
FL
Swings
Jean
J
Christen
Richard
R
D000332:Aerobiosis; D000464:Alginates / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005407:Flagella; D041981:Gastrointestinal Tract / Q000382:microbiology; D020459:Genes, rRNA; D005942:Gluconates / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating) / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008969:Molecular Sequence Data; D008974:Mollusca / Q000382:microbiology*; D009068:Movement; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014733:Vibrio / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001616:beta-Galactosidase / Q000032:analysis
D000464:Alginates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005942:Gluconates; D006603:Hexuronic Acids; D012336:RNA, Ribosomal, 16S; D020723:Glucuronic Acid; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating); D001616:beta-Galactosidase; D005990:Glycerol; D008358:Mannose; C030691:gluconic acid