title: The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM).
abstract: We studied the effects of 2,3-butanedione monoxime (BDM) on the cytoplasmic streaming of Chara corallina and on the motility of myosin prepared from the same plant to examine whether this reagent really affects the plant class XI myosin. It was found that BDM inhibited both cytoplasmic streaming and the motility of myosin at a very similar concentration range (10-100 mM). BDM introduced directly into tonoplast-free cells also inhibited cytoplasmic streaming. These results suggested that effect of BDM on cytoplasmic streaming was exerted through myosin and not through ion channels at least in Chara corallina, though a very high concentration of BDM was required.
authors.affiliation: Department of Biology, Chiba University, Inage-ku, Chiba, 263-8522 Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Funaki
authors.forename: Ayumi
authors.initials: A
authors.lastname: Nagata
authors.forename: Youka
authors.initials: Y
authors.lastname: Akimoto
authors.forename: Kiyo
authors.initials: K
authors.lastname: Shimada
authors.forename: Kohji
authors.initials: K
authors.lastname: Ito
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D044784:Chara / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology*; D009218:Myosins / Q000378:metabolism / Q000502:physiology*
chemical_list: C004717:diacetylmonoxime; D009218:Myosins; D003931:Diacetyl