title: A successful pregnancy outcome using frozen testicular sperm from a chimeric infertile male with a 46, XX/46, XY karyotype: case report.
abstract: BACKGROUND
  We report a very rare case of the successful delivery of a healthy infant fathered by an infertile chimera.
  
  
  METHODS
  ICSI was performed using frozen sperm. The karyotypes of peripheral lymphocytes were examined with a G-banding stain.
  
  
  RESULTS
  Chromosomal analysis prior to ICSI revealed a 46, XX [28]/46, XY [2] karyotype chimera. As an infant, the subject was diagnosed as a true hermaphrodite, and underwent a hysterectomy and oophorectomy. A small number of sperm were found in minced testicular tissue, and they were stored for ICSI.
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first case report of a successful pregnancy and delivery of a healthy infant fathered by an infertile chimera (46, XX/46, XY) following ICSI using frozen testicular sperm.
authors.affiliation: Iwaki Women's Clinic, 3-2, Ookishita, Uchigo, Iwaki, Fukushima, 973-8403, Japan. sugawara@iwaki-fu.or.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sugawara
authors.forename: Yo
authors.initials: Y
authors.lastname: Tokunaga
authors.forename: Machiko
authors.initials: M
authors.lastname: Maeda
authors.forename: Rie
authors.initials: R
authors.lastname: Komaba
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000328:Adult; D002678:Chimera / Q000235:genetics*; D015925:Cryopreservation; D012734:Disorders of Sex Development / Q000150:complications / Q000235:genetics; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007248:Infertility, Male / Q000209:etiology / Q000235:genetics* / Q000628:therapy*; D007621:Karyotyping; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic