title: Comparison of dengue-1 virus envelope glycoprotein gene sequences from French Polynesia.
abstract: Dengue (DEN) is the leading arboviral infection of humans, with 100 million cases annually in the tropical areas of the world. The recent severe DEN-1 epidemic in French Polynesia in 2001, with an incidence rate of 16% and more than 45% of the cases with dengue hemorrhagic fever/dengue shock syndrome among 1,400 hospitalized children and eight fatalities, led us to study this new circulating strain. The entire envelope (E) gene of two French Polynesian DEN-1 virus isolates from the two epidemics of 1988-1989 (FP89) and 2001 (FP01) were sequenced and compared with 29 published DEN-1 virus E gene sequences. Phylogenetic relationships showed that the FP89 strain belonged to genotype V and the FP01 strain to genotype IV based on studies on the same region of DEN-1 virus genome (1,485 nucleotides). The recent dengue epidemic in French Polynesia in 2001 was probably due to the introduction of a new DEN-1 virus from Southeast Asia, since the minimum nucleotide divergence was 3.3% with A88, the Indonesian strain isolated in 1988 in Jakarta.
authors.affiliation: Laboratoire de Recherche en Virologie Médicale, Institut Louis Malardé, PO Box 30, 98713 Papeete Tahiti, Polynésie Française. mlaille@ilm.pf
authors.forename: Manola
authors.initials: M
authors.lastname: Laille
authors.forename: Claudine
authors.initials: C
authors.lastname: Roche
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D006801:Humans; D010802:Phylogeny; D011114:Polynesia / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C072281:E protein TH Sman, Dengue virus