Brugia malayi infection in Meriones unguiculatus: antibody response to recombinant BmR1.
BmR1 recombinant antigen has previously been shown to demonstrate high sensitivity and specificity in the serological diagnosis of brugian filariasis in humans. In this study, the pattern of recognition of antibody to BmR1 during Brugia malayi infection was investigated by employing Meriones unguiculatus as the experimental model. Thirty two gerbils were infected subcutaneously with 120 L(3); and two control groups each comprising 25 animals were employed. ELISA using BmR1 was used to detect filaria-specific IgG antibodies elicited by the gerbils; using sera collected from the day 1 until day 150 post-inoculation (p.i.). The results showed that BmR1 detected B. malayi infection in gerbils harboring adult worms irrespective of the presence of circulating microfilaria, and was exemplified by positive ELISA results in nine a microfilaraemic animals that harbored live adult worms. The initial time of the antibody recognition was at day 8 p.i. and the antibody titre showed some correlation with adult worm burden.
Lim
Boon Huat
BH
Institute for Research in Molecular Medicine and School of Medical Sciences, Universiti Sains Malaysia, Malaysia.
Noordin
Rahmah
R
Nor
Zurainee Mohamed
ZM
Rahman
Rohana Abdul
RA
Abdullah
Khairul Anuar
KA
Sinnadurai
Sivanandam
S
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D017178:Brugia malayi / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis* / Q000276:immunology; D005849:Gerbillinae; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008842:Microfilariae / Q000254:growth & development / Q000302:isolation & purification; D018512:Parasitemia / Q000175:diagnosis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins