Detection of genetically modified organisms by electrochemiluminescence PCR method.
With the development of biotechnology, more and more genetically modified organisms (GMOs) have entered commercial market. Because of the safety concerns, detection and characterization of GMOs have attracted much attention recently. In this study, electrochemiluminescence polymerase chain reaction (ECL-PCR) combined with hybridization technique was applied to detect the GMOs in genetically modified (GM) soybeans and papayas for the first time. Whether the soybeans and the papayas contain GM components was discriminated by detecting the Cauliflower mosaic virus 35S (CaMV35S) promoter. The experiment results show that the detection limit for CaMV35S promoter is 100 fmol, and the GM components can be clearly identified in GM soybeans and papayas. The technique may provide a new means in GMOs detection due to its simplicity and high efficiency.
Liu
Jinfeng
J
Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Xing
Da
D
Shen
Xingyan
X
Zhu
Debin
D
D029441:Carica / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D017796:Caulimovirus / Q000502:physiology; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013025:Glycine max / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000821:virology
D011994:Recombinant Proteins