title: Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity.
abstract: In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.
authors.affiliation: Department of Immunology, Kurume University School of Medicine, Asahi-machi, Kurume, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shichijo
authors.forename: N
authors.initials: N
authors.lastname: Keicho
authors.forename: H T
authors.initials: HT
authors.lastname: Long
authors.forename: T
authors.initials: T
authors.lastname: Quy
authors.forename: N C
authors.initials: NC
authors.lastname: Phi
authors.forename: L D
authors.initials: LD
authors.lastname: Ha
authors.forename: V V
authors.initials: VV
authors.lastname: Ban
authors.forename: S
authors.initials: S
authors.lastname: Itoyama
authors.forename: C-J
authors.initials: CJ
authors.lastname: Hu
authors.forename: N
authors.initials: N
authors.lastname: Komatsu
authors.forename: T
authors.initials: T
authors.lastname: Kirikae
authors.forename: F
authors.initials: F
authors.lastname: Kirikae
authors.forename: S
authors.initials: S
authors.lastname: Shirasawa
authors.forename: M
authors.initials: M
authors.lastname: Kaji
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Sata
authors.forename: T
authors.initials: T
authors.lastname: Kuratsuji
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Sasazuki
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007107:Immune System / Q000276:immunology*; D007109:Immunity / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D010455:Peptides / Q000276:immunology*; D012212:Rheology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D044967:Serum / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D010455:Peptides