Improved survival of microencapsulated islets during in vitro culture and enhanced metabolic function following transplantation.
OBJECTIVE
The aim of this study was to determine whether a simple alginate capsule can prolong islet survival and function during long-term tissue culture. We also wanted to observe the ability of these encapsulated islets to restore glucose responsiveness to diabetic recipients, along with the quantity of islets required to do so.


METHODS
We compared the recovery and metabolic function of encapsulated canine islets with that of non-encapsulated canine islets following 1, 2 or 3 weeks of tissue culture. These culture preparations were also transplanted into diabetic nude mice and compared for their ability to reverse diabetes. Furthermore, short-term cultured encapsulated and non-encapsulated islets were transplanted in varying numbers to determine the minimum dose required to normalise blood glucose and prolong recipient survival.


RESULTS
Islet recovery following 1, 2 and 3 weeks of tissue culture was significantly higher when islets were encapsulated. When these islets were recovered at 1, 2 and 3 weeks and transplanted into diabetic nude mice, survival at 100 days was 100% for all encapsulated groups, versus 66%, 33% and 33% respectively for the non-encapsulated islets. Additionally, substantially fewer short-term cultured islets were required to normalise blood glucose when the islets were encapsulated. Recipients of encapsulated islets also had significantly longer survival times than recipients of non-encapsulated preparations.


CONCLUSIONS
This study demonstrates that encapsulation of islets with purified alginate improves islet survival and function in vitro and in vivo.
Korbutt
G S
GS
Surgical-Medical Research Institute, Dentistry/Pharmacy Building, University of Alberta, Edmonton, Alberta, Canada.
Mallett
A G
AG
Ao
Z
Z
Flashner
M
M
Rajotte
R V
RV
D000818:Animals; D002214:Capsules; D018929:Cell Culture Techniques / Q000379:methods; D004285:Dogs; D006085:Graft Survival / Q000502:physiology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D051379:Mice; D023421:Models, Animal; D014184:Transplantation, Homologous
D002214:Capsules; D007328:Insulin