RNAi in a postmodern, postgenomic era.
As dsRNA-mediated interference (RNAi) libraries become more widely available, genome-wide loss of function experiments in cell culture are likely to become a routine laboratory tool. RNAi screens can then be conducted to test gene functions in different assays in a variety of cell types and across species. In comparing these large phenotypic data sets, researchers will gain a new perspective, enabling them to see each gene as part of a fluid network that is remodelled in response to changes in environmental and developmental context, and with the passage of evolutionary time.
Baum
Buzz
B
Ludwig Institute for Cancer Research, UCL Branch, London W1W 7BS, UK. buzz@ludwig.ucl.ac.uk
Craig
Gavin
G
D015723:Gene Library; D023281:Genomics; D033302:Postmodernism; D034622:RNA Interference / Q000502:physiology*; D017422:Sequence Analysis, DNA