title: Effect of roasting history and buffer composition on peanut protein extraction efficiency.
abstract: Peanut is a major allergenic food. Undeclared peanut (allergens) from mis-formulation or contamination during food processing pose a potential risk for sensitized individuals and must be avoided. Reliable detection and quantification methods for food allergens are necessary in order to ensure compliance with food labelling and to improve consumer protection. The extraction of proteins from allergenic foods and complex food products is an important step in any allergen detection method. In this study, the protein extraction efficiency of various buffers prepared in-house and some extraction buffers included in some commercial allergen enzyme-linked immunosorbent assay (ELISA) test kits for peanut determination in food products were tested. In addition, the effect of roasting history on the extractability of peanut protein was investigated by the biuret and the bicinchoninic acid (BCA) assays. Elevated roasting temperatures in food processing were found to have a major impact on protein extraction efficiency by reducing protein yields of oil and dry roasted peanuts by 50-75% and 75-80%, respectively, compared with the raw material. Extraction buffers operating in the higher pH range (pH 8-11) showed best yields.
authors.affiliation: European Commission, DG Joint Research Centre, Institute for Reference Materials and Measurements, Geel, Belgium. roland.poms@icc.or.at
authors.forename: Roland E
authors.initials: RE
authors.lastname: Poms
authors.forename: Claudia
authors.initials: C
authors.lastname: Capelletti
authors.forename: Elke
authors.initials: E
authors.lastname: Anklam
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D010367:Arachis / Q000276:immunology*; D002021:Buffers; D003296:Cooking / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D006801:Humans; D021183:Peanut Hypersensitivity / Q000517:prevention & control*; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D002021:Buffers; D010940:Plant Proteins