[The expression profiles of human chromosome 21 orthologues in mouse M II oocyte].
OBJECTIVE
To analyse the expression profile of orthologues of human chromosome 21 (HC21) in mouse M II oocytes, and to discuss the relationship between this expression profile and early embryonic development and further to find the possible reasons of DS phenotypes genesis.


METHODS
cDNA array and Global RT-PCR methods were used to analyse and identify the expression profile of 93 HC21 orthologues in mouse M II oocytes.


RESULTS
26 of 93 orthologues were proved to be expressed in mouse M II oocytes and these genes were involved in many biological procedure including transcriptional, metabolism, ionic channel, and ubiquitin pathway etc.


CONCLUSIONS
To our knowledge, this is first report about the HC21 orthologues expression profile in M II oocytes and this expression profile indicates that 26 of 93 HC21 orthologues may directly play important role in early development, and the biological information obtained from this experiment would be beneficial to understand imbalance of HC21 genes expression and the molecular mechanism of DS phenotypes genesis.
Yu
Wei-Dong
WD
Department of Obstetrics and Gynaecology, Peking University People's Hospital, Beijing 100044, China.
Liang
Rong
R
Shen
Huan
H
Tian
Li
L
Yang
Li-Jun
LJ
Guo
Jing-Zhu
JZ
D000818:Animals; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004314:Down Syndrome / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D016372:RNA, Antisense / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny; D014158:Transcription, Genetic
D016372:RNA, Antisense