Whole body barometric plethysmography: a screening method to investigate airway reactivity and acute lung injuries in freely moving pigs.
The aim of this work was to investigate whether the Penh index, measured using whole body barometric plethysmography, can be used as a screening parameter to evaluate the airway reactivity and the intensity of the pulmonary response to endotoxins. Penh was firstly recorded in non-sedated freely moving piglets exposed (1) to a nebulized acetylcholine (Ach) pre-treated or not with clenbuterol, or (2) to endotoxin challenge. To measure Penh simultaneously with total pulmonary resistance (R(L)), dynamic compliance (C(dyn)) and intrapleural pressure changes (Max Delta Ppl), an oesophageal balloon catheter technique was used and the piglets were anaesthetised. The recordings were performed during (1) an intravenous metacholine (Mch) challenge and (2) in endotoxin-exposed animals. In freely moving animals, Ach induced a significant dose-dependent increase in Penh, which was significantly blocked by clenbuterol. Endotoxin instillation also resulted in a significant rise in Penh while the corresponding response measured under anaesthesia was significantly and positively correlated with R(L) and Max Delta Ppl. Similar results were obtained during Mch challenge but the Penh was negatively correlated with C(dyn). We conclude that Penh could be used in freely moving piglets as a screening index for airway reactivity and pulmonary functional changes in cholinergic and endotoxin challenges.
Halloy
David J
DJ
Department for Functional Sciences, Unit of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Veterinary Medicine, University of Liège, 4000 Liege, Belgium.
Kirschvink
Nathalie A
NA
Vincke
Grégoire L
GL
Hamoir
Julien N
JN
Delvaux
François H
FH
Gustin
Pascal G
PG
D000403:Airway Resistance; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000662:veterinary*; D001985:Bronchial Provocation Tests / Q000662:veterinary*; D016085:Bronchoconstrictor Agents; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins; D010993:Plethysmography, Whole Body / Q000379:methods / Q000662:veterinary*; D011897:Random Allocation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D016085:Bronchoconstrictor Agents; D004731:Endotoxins