RNA encoding the MPT83 antigen induces protective immune responses against Mycobacterium tuberculosis infection.
We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies.
Xue
Tian
T
Mycobacterial Division, National Institute for Medical Research, London NW7 1AA, UK.
Stavropoulos
Evangelos
E
Yang
Min
M
Ragno
Silvia
S
Vordermeier
Martin
M
Chambers
Mark
M
Hewinson
Glyn
G
Lowrie
Douglas B
DB
Colston
M Joseph
MJ
Tascon
Ricardo E
RE
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D012329:RNA, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012845:Sindbis Virus / Q000201:enzymology / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D012324:RNA-Dependent RNA Polymerase