[Combination of direct-ELISA and PCR for the rapid detection and identification of Salmonella spp].
OBJECTIVE
To develop a protocol for the rapid detection of Salmonellae.


METHODS
A mono-antibody-based direct-ELISA and PCR methods for the detection of Salmonella were developed previously. This study assessed the accuracy of both direct-ELISA and PCR methods for the rapid detection of Salmonella and set up a new detection protocol.


RESULTS
The sensitivity of the PCR method was higher than that of direct-ELISA method. In the 2002 spring physical examination for employees, 1 546 human fecal samples were examined by the combination of direct-ELISA and PCR method. Compared with the results of national standard method, the sensitivity and specificity of direct ELISA was 100% and 97.14%, respectively, while those of PCR method reached both 100%. It also indicated that combination use of two methods could give positive report within 40 hrs, and also achieve high sensitivity and specificity.


CONCLUSIONS
Based on the results obtained, a protocol for the rapid detection of Salmonella was developed. The first step is to us direct-ELISA method to screen the large number of samples, and then use PCR method to validate the ELISA positive samples, and the final step is, if needed, is to use the national standard method to determine the serotypes of Salmonellae.
Huang
Jin-Lin
JL
Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China.
Jiao
Xin-An
XA
Pan
Zhi-Ming
ZM
Wen
Qi-Yi
QY
Sun
Lin
L
Liu
Xiu-Fan
XF
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping