Comprehensive search for chicken W chromosome-linked genes expressed in early female embryos from the female-minus-male subtracted cDNA macroarray.
In order to seek chicken W chromosome-linked genes expressed significantly earlier than the time of gonadal differentiation, female-minus-male-subtracted cDNA macroarrays were prepared from day 2 (Hamburger-Hamilton stages 12-13), day 3 (stages 19-20) and day 4 (stages 24-25) embryos. From a total of 15-744 macroarrayed cDNA clones, 610 clones exhibiting significantly female-specific expression were selected. When each one of the 610 cDNA clones was used as a probe in Southern blot hybridization with male or female chicken genomic DNA, 62 clones, grouped into eight (A-H) types according to their patterns of hybridization, were considered to be derived from W chromosome-linked genes. When representative cDNA clones in each type were sequenced, clones derived from two known W-linked genes; SPIN-W and ATP5A1W , and from two hitherto unknown W-linked genes, represented by 2d-2D9 and 2d-2F9 clones, were identified and their localizations on the W chromosome were confirmed by fluorescence in-situ hybridization. The 2d-2D9 sequence has no significant homology with other genes in databases but 2d-2F9 has a region which shows partial homology to the consensus sequence of the AAA ATPase superfamily. Both 2d-2D9 and 2d-2F9 sequences are found in contigs of undetermined chromosome-linkage in the Draft Chicken Genome Sequence.
Yamada
Daisuke
D
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-8510 Japan.
Koyama
Yoshiyuki
Y
Komatsubara
Mai
M
Urabe
Mayuko
M
Mori
Masako
M
Hashimoto
Yoichi
Y
Nii
Ryosuke
R
Kobayashi
Midori
M
Nakamoto
Ayako
A
Ogihara
Jun
J
Kato
Jun
J
Mizuno
Shigeki
S
D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D012730:Sex Chromosomes
D018076:DNA, Complementary