title: Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections.
abstract: Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue haemorrhagic fever (DHF). To develop a better understanding of the roles of platelet-associated IgG (PAIgG) and IgM (PAIgM) in inducing thrombocytopenia and its severity of disease in patients with secondary dengue virus infection, the relationship between the PAIgG or PAIgM levels and disease severity as well as thrombocytopenia was examined in 78 patients with acute phase secondary infection in a prospective hospital-based study. The decrease in platelet count during the acute phase recovered significantly during the convalescent phase. In contrast, the increased levels of PAIgG or PAIgM that occurred during the acute phase of these patients decreased significantly during the convalescent phase. An inverse correlation between platelet count and PAIgG or PAIgM levels was found in these patients. Anti-dengue virus IgG and IgM activity was found in platelet eluates from 10 patients in an acute phase of secondary infection. Increased levels of PAIgG or PAIgM were significantly higher in DHF than those in dengue fever (DF). An increased level of PAIgM was associated independently with the development of DHF, representing a possible predictor of DHF with a high specificity. Our present data suggest that platelet-associated immunoglobulins involving antidengue virus activity play a pivotal role in the induction of thrombocytopenia and the severity of the disease in secondary dengue virus infections.
authors.affiliation: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Oishi
authors.forename: S
authors.initials: S
authors.lastname: Inoue
authors.forename: E M
authors.initials: EM
authors.lastname: Dimaano
authors.forename: M T P
authors.initials: MT
authors.lastname: Alera
authors.forename: A M P
authors.initials: AM
authors.lastname: Robles
authors.forename: B D
authors.initials: BD
authors.lastname: Estrella
authors.forename: A
authors.initials: A
authors.lastname: Kumatori
authors.forename: K
authors.initials: K
authors.lastname: Moji
authors.forename: M T
authors.initials: MT
authors.lastname: Alonzo
authors.forename: C C
authors.initials: CC
authors.lastname: Buerano
authors.forename: R R
authors.initials: RR
authors.lastname: Matias
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: F F
authors.initials: FF
authors.lastname: Natividad
authors.forename: T
authors.initials: T
authors.lastname: Nagatake
mesh_terms: D000293:Adolescent; D001792:Blood Platelets / Q000276:immunology*; D003715:Dengue / Q000276:immunology*; D006400:Hematocrit / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D010976:Platelet Count; D011446:Prospective Studies; D019595:Severe Dengue / Q000276:immunology; D012720:Severity of Illness Index; D013921:Thrombocytopenia / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M