Gene expression spectra in human leukemia HL-60 cells treated with EGCG.
To decipher the molecular mechanism of EGCG induced HL-60 cell apoptosis, alterations of gene expression spectra in HL-60 cell line cells after treatment with EGCG were screened by cDNA chip, and analyzed with the GenePix 3.0 twice; and the cDNA chip results further identified by RT-PCR. Ninety-seven genes among the total 8398 (1.15%) showed consistent significant differential expression in the duplicated cDNA chip assessments. Thirty-nine genes (40.2%) were up-regulated and 58 genes (59.8%) were down-regulated; and the randomly selected four performed RT-PCR results agreed with the cDNA chip data. The results suggest that the apoptosis of HL-60 cells induced by EGCG is a progressive transformation process regulated by a variety of genes.
Cao
J
J
Tea and Health Laboratory, Central-South University Xiangya School of Medicine, Changsha 410078, Hunan, PR China. caojin@public.cs.hn.cn
Ren
L L
LL
Liu
J W
JW
Li
Y
Y
Qu
H Y
HY
D001483:Base Sequence; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D017931:DNA Primers; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D002392:Catechin; C045651:epigallocatechin gallate