Community-onset disease caused by Citrobacter freundii producing a novel CTX-M beta-lactamase, CTX-M-30, in Canada.
Strains of Citrobacter freundii intermediate to cefotaxime but sensitive to ceftazidime were isolated from four different patients in Canada. Sequencing of PCR products by use of CTX-M-specific primers revealed a new combination of four amino acid substitutions. This new gene was designated bla(CTX-M-30) and was encoded on a 3-kb plasmid. The pI of CTX-M-30 was 8.0.
Abdalhamid
Baha
B
Center for Research in Antiinfectives & Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.
Pitout
Johann D D
JD
Moland
Ellen S
ES
Hanson
Nancy D
ND
D015139:Blotting, Southern; D002170:Canada; D016971:Citrobacter freundii / Q000187:drug effects* / Q000201:enzymology; D017714:Community-Acquired Infections / Q000382:microbiology* / Q000652:urine; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology* / Q000652:urine; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007525:Isoelectric Focusing; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases; C494144:beta-lactamase CTX-M-30, Citrobacter freundii