title: Blood supply and oxidative metabolism in muscle biopsies of female cleaners with and without myalgia.
abstract: OBJECTIVE
  Pathomechanisms of work-related myalgia are poorly understood. Myalgia is thought to be caused by excitation of nociceptors present in the muscular tissue but not in the muscle fiber itself. Muscle contraction in combination with hypoxia is known to excite nociceptors. Morphologic analysis can contribute to the knowledge of the excitation of nociceptors. This study thoroughly examines the morphology of the trapezius muscle's capillary supply and signs of disturbed oxidative metabolism to understand their role in work-related myalgia.
  
  
  METHODS
  Surgical trapezius muscle biopsies were obtained from 25 female cleaners with long-standing work-related myalgia, 25 female cleaners without trapezius myalgia, and 21 healthy teachers. Enzyme and immunohistochemical stainings were performed to highlight fibers with aberrant intermyofibrillar patterns, indicating a disturbed oxidative metabolism (also known as moth-eaten fibers) and a disturbed capillary supply of different fibers.
  
  
  RESULTS
  A significantly lower number of capillaries per fiber area in cleaners suffering from myalgia compared with cleaners without trapezius myalgia was found. Moth-eaten fibers were found in the 3 groups, but these fibers were significantly more prevalent in the groups of cleaners than in the healthy teacher group.
  
  
  CONCLUSIONS
  This work indicates that the capillary supply of trapezius is affected in work-related trapezius myalgia. More studies are needed to understand possible mechanisms that would explain the occurrence of moth-eaten fibers.
authors.affiliation: Department of Occupational and Environmental Medicine, University Hospital, Lund, Sweden. britt.larsson@ymed.lu.se
authors.forename: Britt
authors.initials: B
authors.lastname: Larsson
authors.forename: Jonas
authors.initials: J
authors.lastname: Björk
authors.forename: Fawzi
authors.initials: F
authors.lastname: Kadi
authors.forename: Rolf
authors.initials: R
authors.lastname: Lindman
authors.forename: Björn
authors.initials: B
authors.lastname: Gerdle
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods; D002196:Capillaries / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007797:Laminin / Q000378:metabolism; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000378:metabolism; D009784:Occupational Diseases / Q000378:metabolism / Q000473:pathology*; D011000:Pleurodynia, Epidemic / Q000473:pathology*; D018709:Statistics, Nonparametric
chemical_list: D007797:Laminin; D009243:NAD; C084098:laminin A