title: Microarray analyses uncover UBE1L as a candidate target gene for lung cancer chemoprevention.
abstract: Retinoids, natural and synthetic derivatives of vitamin A, are active in cancer therapy and chemoprevention. We reported previously that all-trans-retinoic acid (RA) treatment prevented carcinogen-induced transformation of immortalized human bronchial epithelial (HBE) cells. To identify cancer chemopreventive mechanisms, immortalized (BEAS-2B), carcinogen-transformed (BEAS-2B(NNK)), and RA-chemoprevented (BEAS-2B(NNK/RA)) HBE cells were used to conduct microarray analyses independently. Species increased in chemoprevented as compared with immortalized HBE cells (group I) and those augmented in chemoprevented as compared with transformed HBE cells (group II) included known RA-target genes as well as previously unrecognized RA-target genes in HBE cells. Unexpectedly, both groups were also enriched for interferon-stimulated genes. One interferon-stimulated gene of particular interest was UBE1L, the ubiquitin-activating enzyme E1-like protein. UBE1L expression was also induced after prolonged RA-treatment of immortalized HBE cells. UBE1L mRNA was shown previously as repressed in certain lung cancer cell lines, directly implicating UBE1L in lung carcinogenesis. Notably, UBE1L immunoblot expression was reduced in a subset of malignant as compared with adjacent normal lung tissues that were examined. Immunohistochemical analyses were performed using a new assay developed to detect this species using rabbit polyclonal anti-UBE1L antibodies independently raised against the amino- or carboxyl-termini of UBE1L. Studies done on paraffin-embedded and fixed tissues revealed abundant UBE1L, but low levels of cyclin D1 expression in the normal human bronchial epithelium, indicating an inverse relationship existed between these species. To study this further, cotransfection into HBE cells of wild-type or mutant UBE1L species was accomplished. In a dose-dependent manner, wild-type but not mutant UBE1L species repressed cyclin D1 expression. This implicated UBE1L in a retinoid chemoprevention mechanism involving cyclin D1 repression described previously. Taken together, these findings directly implicate UBE1L as a candidate-pharmacologic target for lung cancer chemoprevention. These findings also provide a mechanistic basis for the tumor suppressive effects of UBE1L through cyclin D1 repression.
authors.affiliation: Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Pitha-Rowe
authors.forename: W Jeffrey
authors.initials: WJ
authors.lastname: Petty
authors.forename: Qing
authors.initials: Q
authors.lastname: Feng
authors.forename: Petra H
authors.initials: PH
authors.lastname: Koza-Taylor
authors.forename: Debra A
authors.initials: DA
authors.lastname: Dimattia
authors.forename: Lynn
authors.initials: L
authors.lastname: Pinder
authors.forename: Konstantin H
authors.initials: KH
authors.lastname: Dragnev
authors.forename: Natalie
authors.initials: N
authors.lastname: Memoli
authors.forename: Vincent
authors.initials: V
authors.lastname: Memoli
authors.forename: Tom
authors.initials: T
authors.lastname: Turi
authors.forename: Jean
authors.initials: J
authors.lastname: Beebe
authors.forename: Sutisak
authors.initials: S
authors.lastname: Kitareewan
authors.forename: Ethan
authors.initials: E
authors.lastname: Dmitrovsky
mesh_terms: D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000517:prevention & control*; D020411:Oligonucleotide Array Sequence Analysis; D014212:Tretinoin / Q000627:therapeutic use*; D044764:Ubiquitin-Activating Enzymes / Q000032:analysis / Q000235:genetics* / Q000502:physiology
chemical_list: D016588:Anticarcinogenic Agents; C471538:UBA1 protein, human; D014212:Tretinoin; D044764:Ubiquitin-Activating Enzymes