[Introdoction of bioluminescence genes into silicate-dissolving bacteria strain NBT].
In this study, silicate-dissolving bacteria NBT strain was grown with 0.25% maltose as carbon source, and the rifampicin-resistance was generated by ultraviolet mutagenesis and streak naturalized to 200 microg x ml(-1). Through triparental cross, the luxAB genes were introduced into NBT-R200 at the help of pRK2013. The luminescence activity of the hybrid strain was tested, which indicated that all colonies had a high luminescence activity and kanamycin-, tetracyctine-and rifampicin-resistance. The NBT-R200 cells prepared from initial logarithmic growth phage were more likely to be sensitive to foreign DNA, and the maximum translocation frequency was up to 6.70 x 10(-5). In addition, the optimal mating ratio was 1:1:1. The potassium release ability from feldspar and the luminescence of luxAB genes marked silicate-dissolving bacteria RL85 strain were stable, and hence, the RL85 was available to rhizosphere microecology researches.
He
Linyan
L
Department of Microbiology, Nanjing Agricultural University, Nanjing 210096, China. hlyan0974@yahoo.com.cn
Huang
Weiyi
W
D001419:Bacteria / Q000145:classification; D049449:Luminescence; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D012230:Rhizobiaceae / Q000145:classification / Q000187:drug effects / Q000254:growth & development*; D017640:Silicates; D012988:Soil Microbiology
D017640:Silicates; D009580:Nitroblue Tetrazolium