title: New approach to the analysis of oxidized triacylglycerols in lipoproteins.
abstract: The oxidation of human LDL lipids and the structures of oxidized TAG molecules found in LDL were investigated. Pooled samples of 10 normolipidemic and 10 hyperlipidemic subjects were analyzed. For determination of the oxidation levels, the LDL baseline diene conjugation (LDL-BDC) method was used. A method based on HPLC and electrospray ionization-MS was optimized and applied to the analysis of molecular structures of oxidized TAG in LDL. Differences were found between the oxidation levels of the samples. The LDL-BDC value was 22.2 micromol/L serum in the normolipidemic group, and 88.1 micromol/L serum in the hyperlipidemic group. The amounts of oxidized TAG molecules were small. However, several species of oxidized TAG were identified. These included TAG molecules with a keto or an epoxy group attached to a FA, and TAG molecules with a FA core aldehyde. In some TAG, the keto/epoxy ratio was greater in the hyperlipidemic group compared to the normolipidemic group. The results show that our approach is applicable to research on lipid oxidation in lipoproteins.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. jusuom@utu.fi
authors.forename: Jukka-Pekka
authors.initials: JP
authors.lastname: Suomela
authors.forename: Markku
authors.initials: M
authors.lastname: Ahotupa
authors.forename: Olli
authors.initials: O
authors.lastname: Sjövall
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Kurvinen
authors.forename: Heikki
authors.initials: H
authors.lastname: Kallio
mesh_terms: D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry*; D008722:Methods; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization; D014280:Triglycerides / Q000032:analysis*
chemical_list: D008074:Lipoproteins; D014280:Triglycerides