title: Crystallization of a core fragment of the flagellar hook protein FlgE.
abstract: A core fragment of the bacterial flagellar hook protein FlgE was overexpressed, purified and crystallized. The crystal diffracted to 1.6 A resolution using synchrotron X-radiation. The crystal belongs to the orthorhombic crystal system, with space group P2(1)2(1)2 and unit-cell parameters a = 128.4, b = 48.8, c = 96.7 A. SeMet protein was also overexpressed, purified, crystallized and a set of 2.3 A MAD data was collected.
authors.affiliation: Dynamic NanoMachine Project, ICORP, JST, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Fadel A
authors.initials: FA
authors.lastname: Samatey
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsunami
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imada
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Nagashima
authors.forename: Keiichi
authors.initials: K
authors.lastname: Namba
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010446:Peptide Fragments / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C097681:FlgE protein, Bacteria; D010446:Peptide Fragments