Strategies for measurement of biotransformation enzyme gene expression.
The analysis of gene expression is an integral part of any research characterizing gene function. A wide variety of techniques have been developed for this purpose, each with their own advantages and limitations. This chapter seeks to provide an overview of some of the most recent as well as conventional methods to quantitate gene expression. These approaches include Northern blot analysis, ribonuclease protection assay (RPA), reverse transcription polymerase chain reaction, expressed sequence tag (EST) sequencing, differential display, cDNA arrays, and the serial analysis of gene expression (SAGE). Current applications of the information derived from gene expression studies require assays to be adaptable for the quantitative analysis of a large number of samples and end points within a short period coupled with cost effectiveness. A comparison of some of these features of each analytical approach as well as their advantages and disadvantages has also been provided.
Romkes
Marjorie
M
Center for Clinical Pharmacology, University of Pittsburgh, PA, USA.
Buch
Shama C
SC
D000818:Animals; D001711:Biotransformation / Q000235:genetics*; D015152:Blotting, Northern; D004798:Enzymes / Q000096:biosynthesis / Q000235:genetics*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis
D004798:Enzymes; D012333:RNA, Messenger