Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle.
To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.
Le Roch
Karine G
KG
Department of Cell Biology ICND202, the Scripps Research Institute, La Jolla, California 92037, USA.
Johnson
Jeffrey R
JR
Florens
Laurence
L
Zhou
Yingyao
Y
Santrosyan
Andrey
A
Grainger
Munira
M
Yan
S Frank
SF
Williamson
Kim C
KC
Holder
Anthony A
AA
Carucci
Daniel J
DJ
Yates
John R
JR
Winzeler
Elizabeth A
EA
D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics; D017125:Genes, Protozoan; D008018:Life Cycle Stages / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016053:RNA, Protozoan / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan