[The study of expression of proliferating cell nuclear antigen and its gene in hypopharyngeal carcinoma].
OBJECTIVE
To study the relationship of proliferating cell nuclear antigen (PCNA) and the biological and clinical characteristics of hypopharyngeal carcinoma and its value of clinical prognosis.


METHODS
The SABC-immunohistochemical method and in situ hybridization were correspondingly used to detect the expression of PCNA and its gene in 48 cases of cancer tissue and 15 cases of nearby cancer tissue of primary hypopharyngeal carcinoma. The number of positive cells of PCNA in 63 cases of tissue was represented by proliferating index (PI).


RESULTS
The expression of PCNA correlated with the clinical stage, histological grade and clinical recurrence of hypopharyngeal carcinoma and prognosis of the patients, but did not correlate significantly with tumor location and whether metastasis of cervical lymph nodes or not.


CONCLUSIONS
The expression of PCNA protein may provide a reference in evaluating the malignancy level of hypopharyngeal carcinoma and prognosis of the patients.
Yao
Liansheng
L
Department of Otorhinolaryngology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515.
Li
Xiangping
X
Zhang
Jinhua
J
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis
D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger