title: The advanced glycation end-product N epsilon-(carboxymethyl)lysine level is elevated in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.
abstract: Oxidative stress is involved in the aetiopathogenesis of amyotrophic lateral sclerosis (ALS), a fatal degenerative disorder. To test whether oxidative stress in ALS is increased and confined to the central nervous system, we have measured the glycoxidation product N(epsilon)-(carboxymethyl)lysine (CML) in serum and cerebrospinal fluid (CSF) samples by means of a novel enzyme immunoassay. Significant increases of CSF/serum ratio of CML in ALS patients (n = 25) as compared to normal controls (n = 20, p = 0.001) and to Alzheimer disease patients (n = 9, p = 0.029) suggest intrathecal production of this glycoxidation product. Measurement of CML levels may provide a novel diagnostic tool and may supplement current monitoring strategies in interventional trials.
authors.affiliation: Department of Neurology, University of Ulm, Oberer Eselsberg 45, D-89081 Ulm, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Kaufmann
authors.forename: B O
authors.initials: BO
authors.lastname: Boehm
authors.forename: S D
authors.initials: SD
authors.lastname: Süssmuth
authors.forename: R
authors.initials: R
authors.lastname: Kientsch-Engel
authors.forename: A
authors.initials: A
authors.lastname: Sperfeld
authors.forename: A C
authors.initials: AC
authors.lastname: Ludolph
authors.forename: H
authors.initials: H
authors.lastname: Tumani
mesh_terms: D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid*; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D008239:Lysine / Q000097:blood / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric
chemical_list: D017127:Glycation End Products, Advanced; D008239:Lysine