Hematopoietic and endothelial development of mouse embryonic stem cells in culture.
Embryonic stem (ES) cells differentiate efficiently in vitro and give rise to many different somatic cell types. Hematopoietic progenitors present within differentiated ES cells (embryoid bodies, EBs) can be identified by replating EB cells into semisolid media with hematopoietic growth factors. The developmental kinetics of various hematopoietic lineage precursors within EBs and molecular and cellular studies of these cells have suggested that the sequence of events leading to the onset of hematopoiesis within EBs is similar to that found within the mouse embryo. Thus, the in vitro differentiation model of ES cells provides a unique opportunity to study onset mechanisms involved in hematopoietic development.
Choi
Kyunghee
K
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
Chung
Yun Shin
YS
Zhang
Weh Jie
WJ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D051379:Mice; D039901:Totipotent Stem Cells / Q000502:physiology*