title: Influence of amphotericin B on the ciliary beat frequency of nasal mucosa.
abstract: OBJECTIVE
  A new therapeutic approach in eosinophilic fungal rhinosinusitis is topical application of antifungals such as amphotericin B. The tolerability of a recently applied concentration of this drug by human nasal mucosa was tested in vitro.
  
  
  METHODS
  Ciliary beat frequency (CBF) was measured by a photometric technique, combining a light microscope, a photometer, a photograph-multiplier, and a computed analyzing unit.
  
  
  RESULTS
  Treatment for 20 minutes with amphotericin B finally diluted in saline to 0.1 mg/mL caused no decrease in CBF. By contrast, both amphotericin B diluted in distilled water and distilled water without additives lowered CBF about 50%. This decrease was not reversible after reincubation for 20 minute in saline.
  
  
  CONCLUSIONS
  The results confirm the good tolerability of topical amphotericin B in the nasal and paranasal cavity. Because of the negative effect of distilled water, final dilution in physiologic solvents should be considered.
authors.affiliation: Department of Otorhinolaryngology, University Hospital of Innsbruck, Innsbruck, Austria.
authors.forename: Edmund
authors.initials: E
authors.lastname: Hofer
authors.forename: Andreas
authors.initials: A
authors.lastname: Neher
authors.forename: Anneliese
authors.initials: A
authors.lastname: Schrott-Fischer
authors.forename: Markus
authors.initials: M
authors.lastname: Nagl
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D009068:Movement / Q000187:drug effects / Q000502:physiology; D009297:Nasal Mucosa / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B