title: Detection and quantification of the rare latently infected cell undergoing herpes simplex virus transcriptional activation in the nervous system in vivo.
abstract: Herpes simplex virus (HSV), in contrast to most other members of the herpes virus family, has the ability to infect, enter latency, and reactivate from latency in a number of nonhuman species, including mice. This provides a unique opportunity to study the complex lytic-latent cycle of a human neurotropic virus in a mouse model. This chapter details basic methods for inducing and quantifying reactivation, with emphasis on the first strategy for detecting and quantifying the initiation of HSV reactivation in vivo.
authors.affiliation: Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
authors.forename: Nancy M
authors.initials: NM
authors.lastname: Sawtell
mesh_terms: D000818:Animals; D006561:Herpes Simplex / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D051379:Mice; D009420:Nervous System / Q000821:virology*; D018139:Simplexvirus / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*; D012668:Trigeminal Ganglion / Q000821:virology; D017735:Virus Latency / Q000502:physiology*