title: Oxidized low-density lipoproteins accumulate in rat lung after experimental lung edema induced by alpha- naphthylthiourea (ANTU).
abstract: Oxidation of the low-density lipoprotein (LDL) results in the production of modified LDLs. Oxidation of LDL cholesterol plays a role on the pathogenesis of endothelial dysfunction. This study was designed to investigate the possible participation of the oxidative modification of low density lipoprotein in the lung edema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent on the lung endothelium. When ANTU injected intraperitoneally into rats (15 mg kg(-1)), it produced lung edema as indicated by an increase in lung weight/body weight (LW/BW) ratio and pleural effusion (PE) reaching a maximum within 4 h. A significant lung edema was observed 4 h after intraperitoneally injection of alpha-naphthylthiourea when compared with olive oil-injected control rats. On microscopic examination of alpha-naphthylthiourea-treated rats were shown to have severe lung injury, while no change was observed in olive oil-treated control rats. While there were no staining in control lungs, positive oxidized low-density lipoproteins immune-fluorescent staining were observed in lung edema group. Our study showed that oxidized low-density lipoprotein (oxLDL) accumulated in ANTU-induced lung damage. This is the first study in which accumulation of oxLDL molecules in the intact lung tissue were shown by fluorescent immune-staining method in experimental lung edema. The potential role of oxLDL in this pathology are still under investigation.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Karaelmas University, 67100 Incivez Zonguldak, Turkey. dresipahi@yahoo.com
authors.forename: Emine Yilmaz
authors.initials: EY
authors.lastname: Sipahi
authors.forename: Ishak
authors.initials: I
authors.lastname: Ozel Tekin
authors.forename: Mustafa
authors.initials: M
authors.lastname: Comert
authors.forename: Figen
authors.initials: F
authors.lastname: Barut
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ustun
authors.forename: Tunc Hakan
authors.initials: TH
authors.lastname: Sipahi
mesh_terms: D000818:Animals; D005260:Female; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000378:metabolism; D008168:Lung / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011654:Pulmonary Edema / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D013890:Thiourea / Q000031:analogs & derivatives* / Q000633:toxicity*
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D013890:Thiourea; C005277:alpha-naphthyl thiourea