Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and smoking.
Mutant frequency at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene in the peripheral blood lymphocytes obtained from 44 healthy individuals (23 non-smokers and 21 smokers) of an Indian male population was studied using T-lymphocyte cloning assay. It was found that lnMF increased with age at a rate of 2.5% per year (P <0.001). Blood samples from smokers showed a significant (P <0.037) increase in HPRT mutant frequency (MF) (10.43 +/- 4.74 x 10(-6)) as compared to that obtained from non-smokers (7.69 +/- 3.69 x 10(-6)). This study also showed a significant (P <0.027) inverse correlation between lnMF and non-selected cloning efficiency (CE). However, with respect to age no variation was observed in cloning efficiency. The results obtained in this study showed a good comparison with those reported in different populations of the world.
Kumar
P R Vivek
PR
Radiological Safety Division, Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam-603102, India.
Hamza
V Zareena
VZ
Mohankumar
Mary N
MN
Jeevanram
R K
RK
D000328:Adult; D000367:Age Factors; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007194:India; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012907:Smoking / Q000235:genetics*; D013601:T-Lymphocytes / Q000201:enzymology*
D007041:Hypoxanthine Phosphoribosyltransferase