Release of nucleophosmin from the nucleus: Involvement in aloe-emodin-induced human lung non small carcinoma cell apoptosis.
Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is one of the active constituents from the root and rhizome of Rheum palmatum. Our previous study has demonstrated that aloe-emodin induced a significant change in the expression of lung cancer cell apoptosis-related proteins compared to those of control cells. However, the molecular mechanisms underlying the biological effects of aloe-emodin still remain unknown. Based on these reasons, we were interested in the change of aloe-emodin-induced total protein expression by the proteomics technique during aloe-emodin-induced lung cancer cell apoptosis. Our study applied 2D electrophoresis to analyze the proteins involved in aloe-emodin-induced apoptosis in H460 cells. We found that the release of nucleophosmin from the nucleus to the cytosol and the degradation of nucleophosmin were associated with aloe-emodin-induced H460 cell apoptosis. Our study also demonstrated that the gene expression of nucleophosmin remained unchanged after treatment with aloe-emodin. The aloe-emodin-caused increase in the amount of proform and fragment of nucleophosmin in cytoplasm may be one of the important events for aloe-emodin-induced H460 cell apoptosis.
Lee
Hong-Zin
HZ
School of Pharmacy, China Medical University, Taichung, Taiwan. hong@mail.cmu.edu.tw
Wu
Chun-Hsiung
CH
Chang
Shen-Peng
SP
D000595:Amino Acid Sequence; D000880:Anthraquinones; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D004642:Emodin / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D000090243:Nucleophosmin; D010446:Peptide Fragments / Q000737:chemistry
D000880:Anthraquinones; D002273:Carcinogens; D004791:Enzyme Inhibitors; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D010446:Peptide Fragments; D000090243:Nucleophosmin; C518327:aloe emodin; D004642:Emodin