title: Effect of beta-casein (1-28) on proliferative responses and secretory functions of human immunocompetent cell lines.
abstract: The effect of bovine beta-casein (1-28) purified from commercial casein phosphopeptide preparations on human T, B, and monocyte cell lines was evaluated. Beta-casein (1-28) enhanced the proliferation of the following: T cell lines HUT-78, Jurkat Clone E6-1, and MOLT-4; B cell lines BALL, KHM-1B, and U266B1; and monocyte cell lines U937 and HL-60. Moreover, beta-casein (1-28) stimulated IgA production by KHM-1B over 96 h of culture. Semiquantitative reverse transcriptional-polymerase chain reaction analysis indicated that beta-casein (1-28) enhanced mRNA expression of interleukin (IL)-6 in U266B1 and KHM-1B. These results suggest that beta-casein (1-28) exerts a mitogenic effect on human T, B, and monocyte cells, and an IgA-enhancing effect on B cells.
authors.affiliation: Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, Kamiina, Nagano 399-4598, Japan. tkawafb@shinshu-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawahara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Katayama
authors.forename: Hajime
authors.initials: H
authors.lastname: Otani
mesh_terms: D000818:Animals; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D015850:Interleukin-6 / Q000378:metabolism; D019169:Jurkat Cells; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D020298:U937 Cells
chemical_list: D002364:Caseins; D007070:Immunoglobulin A; D015850:Interleukin-6; D012333:RNA, Messenger