Epidermal cell analysis by RT-PCR.
Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.
Troy
Tammy-Claire
TC
Ottawa Health Research Institute, Ottawa, Ontario, Canada.
Cheung
Robert Man-Kit
RM
Turksen
Kursad
K
D000818:Animals; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D012333:RNA, Messenger