A role of anti-verotoxin antibody immunoreactive peptide, Virp5, from rat spinal cord.
An anti-verotoxin 2 (VT2) antibody immunoreactive 5-kDa polypeptide (Virp5), has been obtained through screening of the rat spinal cord cDNA library with the aid of anti-VT2 antibody. Virp5 was mainly expressed in the central nervous system, liver and kidney, and localized at glia-like cells and nerve fibers in the central nervous system, vascular endothelial cells and hepatic cells in the liver, as well as epithelial cells of distal tubules in the kidney. Intravenous administration of purified Virp5 elicited a dose-dependent increase in blood pressure. These results suggest that Virp5 commonly exists in the body, being partly playing a role in regulating the blood pressure.
Ishikawa
Naohisa
N
Department of Pharmacology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan. nao@aichi-med-u.ac.jp
Feng
Guo-Gang
GG
Ito
Yoshitake
Y
Hotta
Yoshihiro
Y
Wakida
Yasushi
Y
Murakami
Hidetsugu
H
Yajima
Michio
M
Ishikawa
Atsuko
A
Yokochi
Takashi
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure / Q000187:drug effects; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D015723:Gene Library; D006339:Heart Rate / Q000187:drug effects; D022781:Hepatocytes / Q000378:metabolism; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009412:Nerve Fibers / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D022641:Shiga Toxin 2 / Q000276:immunology*; D013116:Spinal Cord / Q000737:chemistry* / Q000276:immunology
D018076:DNA, Complementary; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; D022641:Shiga Toxin 2; C499790:Virp5 protein, rat