Inhibitory activity of Chrysanthemi sibirici herba extract on RBL-2H3 mast cells and compound 48/80-induced anaphylaxis.
The effects of extracts from various oriental medicinal herbs on mast cell-mediated allergic reaction were investigated. Among them, Chrysanthemi sibirici herba ethanol extract exerted the potent inhibitory activity on antigen-induced degranulation in RBL-2H3 mast cells. Chrysanthemi sibirici herba dose-dependently inhibited DNP-BSA or compound 48/80-induced degranulation in RBL-2H3 mast cells, with IC(50) values of approximately 49 microg/ml and 76 microg/ml, respectively. This extract strongly suppressed compound 48/80-induced systemic anaphylaxis by 48.7% at a dose of 300 mg/kg in mice. Chrysanthemi sibirici herba also inhibited the expression of TNF-alpha and the activation of the MAP kinase, ERK1/2, which is critical for the production of inflammatory cytokines in mast cells, as indicated by the suppression of activating phosphorylation of ERK1/2. These results lead us to conclude that Chrysanthemi sibirici herba may be used clinically to treat various allergic diseases.
Lee
Jun Ho
JH
Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, Republic of Korea.
Seo
Jin Young
JY
Ko
Na Young
NY
Chang
Sung Ho
SH
Her
Erk
E
Park
Taekyu
T
Lee
Hoi Young
HY
Han
Jeung Whan
JW
Kim
Young Mi
YM
Choi
Wahn Soo
WS
D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology; D031202:Chrysanthemum; D004305:Dose-Response Relationship, Drug; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000302:isolation & purification / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000633:toxicity*
D010936:Plant Extracts; D028321:Plant Preparations; D003189:p-Methoxy-N-methylphenethylamine