title: Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.
abstract: A protocol for detection of mutations in the TP53 gene using temporal temperature gradient gel electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques denaturing gradient gel electrophoresis (DGGE) and constant denaturant gel electrophoresis (CDGE) and eliminates some of the problems. The result is a rapid and sensitive screening technique that is robust and easily set up in smaller laboratory environments.
authors.affiliation: Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Therese
authors.initials: T
authors.lastname: Sørlie
authors.forename: Hilde
authors.initials: H
authors.lastname: Johnsen
authors.forename: Phuong
authors.initials: P
authors.lastname: Vu
authors.forename: Guro Elisabeth
authors.initials: GE
authors.lastname: Lind
authors.forename: Ragnhild
authors.initials: R
authors.lastname: Lothe
authors.forename: Anne-Lise
authors.initials: AL
authors.lastname: Børresen-Dale
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics; D009691:Nucleic Acid Denaturation; D013696:Temperature
chemical_list: D004247:DNA