[Expression and significance of caspase-3 gene in apoptotic muscle cells 103Pd radioactive stent bile duct in dogs].
OBJECTIVE
To discuss the expression and significance of caspase-3 gene in the apoptotic muscle cells in gamma-radiation-induced muscle cell lines.


METHODS
The caspase-3 mRNA in the control and gamma-radiation induced apoptotic muscle cells was analysed by RT-PCR.


RESULTS
The expression of caspase-3 gene transcript was higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct, and apoptotic muscle cells were higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct.


CONCLUSIONS
The high level expression of caspase-3 gene may help to understand the muscle cells sensitivity to gamma-radiation apoptosis. 103Pd radioactive stent may increase the expression of caspase-3 gene in dog bile duct and prevent the billiary narrow when dog bile duct was injured by balloon.
He
Gui-Jin
GJ
Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China.
Su
Qi
Q
Gao
Qin-Yi
QY
Xu
Shu-He
SH
Gao
Hong
H
Jiang
Wei-Guo
WG
Jiang
Tao
T
Dai
Xian-Wei
XW
Ma
Kai
K
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D051017:Apoptosis Regulatory Proteins; D001652:Bile Ducts / Q000201:enzymology / Q000528:radiation effects*; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000528:radiation effects*; D004285:Dogs; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000528:radiation effects*; D010165:Palladium / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000235:genetics / Q000528:radiation effects; D011868:Radioisotopes / Q000008:administration & dosage*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015607:Stents
D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger; D011868:Radioisotopes; D010165:Palladium; D053148:Caspase 3; D020169:Caspases