[Expression of VEGF in endothelial cells and the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside].
OBJECTIVE
To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304) and the inhibitory effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (ST I) in vitro.


METHODS
Exposure to 2.5 mg x L(-1) LPC or LPC + ST I for 24 hours, VEGF protein was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, VEGF mRNA expression in ECV304 was examined by in situ hybridization. VEGF165 mRNA was examined by RT-PCR and Realtime RT-PCR.


RESULTS
LPC upregulated VEGF protein and VEGF mRNA expression in the ECV304 cells. ST I was shown to markedly inhibit the LPC-induced increase of VEGF protein and VEGF165 mRNA (P < 0.001).


CONCLUSIONS
LPC can induce a strong expression of VEGF in ECV304 cells and ST I can inhibit it.
Zhang
Li
L
Eastern Hepatobiliary Hospital, The Second Military Medical University, Shanghai 200433, China.
Rui
Yao-cheng
YC
Qiu
Yan
Y
Li
Tie-jun
TJ
Liu
Hou-jia
HJ
Chen
Wan-sheng
WS
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D005960:Glucosides; D008244:Lysophosphatidylcholines; D012333:RNA, Messenger; D013267:Stilbenes; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A