title: An assay of mammalian cell micropermeabilization based on measurements of cellular lactate production.
abstract: A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precision similar to that of micropermeabilization assays based on the release of 86Rb+ from preloaded cells, avoidance of radioactivity, and simplicity of the measurements and equipment required.
authors.affiliation: Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA. elehoux@biochem.okstate.edu
authors.forename: Eric A
authors.initials: EA
authors.lastname: Lehoux
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Baker
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Bush
authors.forename: H Olin
authors.initials: HO
authors.lastname: Spivey
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D006019:Glycolysis; D019344:Lactic Acid / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D010758:Phosphorus / Q000494:pharmacology; D051381:Rats; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D010758:Phosphorus; D019344:Lactic Acid