Cleavage of the N-linked oligosaccharide from the surfaces of Chlamydia species affects attachment and infectivity of the organisms in human epithelial and endothelial cells.
Previous studies demonstrated that the high-mannose oligosaccharide N linked to the Chlamydia major outer membrane protein inhibited the attachment and infectivity of the organism. The present study showed that cleavage of the glycan with N-glycanase decreased the attachment and infectivity of chlamydial organisms in human epithelial and endothelial cells.
Kuo
Cho-chou
CC
Department of Pathobiology, Box 357238, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu
Lee
Amy
A
Campbell
Lee Ann
LA
D001422:Bacterial Adhesion; D002460:Cell Line; D002689:Chlamydia / Q000145:classification / Q000502:physiology*; D042783:Endothelial Cells / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism
D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases; C020668:glycanase