[FCGR3B gene frequencies and FCGR3 gene variants in Chinese: a study in 487 Chinese individuals from Zhejiang Province].
OBJECTIVE
To investigate the FCGR3B gene frequencies and FCGR3 variants in a Chinese population from Zhejiang Province.


METHODS
DNA was extracted from the blood specimens of 487 healthy blood donors from Zhejiang Province. The FCGR3B gene frequencies were determined by polymerase chain reaction with sequence-specific primers (PCR-SSP). The 19 specimens with 3 different FCGR3B genotypes underwent FCGR3 gene cloning and sequencing.


RESULTS
The gene frequencies of FCGR3B(*1), FCGR3B(*2), and FCGR3B(*3) in these 487 individuals were 0.564, 0.429, and 0.000 respectively. The genotype frequency of FCGR3B(null) was 0.62% (3/487). Sequencing of FCGR3 revealed that in 7 out of the 19 Chinese individuals variants caused by single nucleotide exchanges at one or more of the polymorphic positions 141, 147, 227, 266 and 277 in exon 3 also existed in this Chinese population.


CONCLUSIONS
FCGR3B(*1) gene is more frequent in a Chinese population from Zhejiang Province than the FCGR3B(*2) gene, and the FCGR3B(*3) gene seems to be absent. Gene variants caused by single nucleotide exchanges are found in addition to the well known forms, but the reason for this remains unclear.
Tong
Yin
Y
Department of Hematology, First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
Jin
Jie
J
Wu
Jun-Feng
JF
Wang
Yun-Gui
YG
Wang
Lei
L
Neppert
Juergen
J
Flesch
Brigitte
B
D015703:Antigens, CD / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D058851:GPI-Linked Proteins; D015870:Gene Expression; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017452:Receptors, IgG / Q000235:genetics*
D015703:Antigens, CD; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG