title: Computational modeling reveals how interplay between components of a GTPase-cycle module regulates signal transduction.
abstract: Heterotrimeric G protein signaling is regulated by signaling modules composed of heterotrimeric G proteins, active G protein-coupled receptors (Rs), which activate G proteins, and GTPase-activating proteins (GAPs), which deactivate G proteins. We term these modules GTPase-cycle modules. The local concentrations of these proteins are spatially regulated between plasma membrane microdomains and between the plasma membrane and cytosol, but no data or models are available that quantitatively explain the effect of such regulation on signaling. We present a computational model of the GTPase-cycle module that predicts that the interplay of local G protein, R, and GAP concentrations gives rise to 16 distinct signaling regimes and numerous intermediate signaling phenomena. The regimes suggest alternative modes of the GTPase-cycle module that occur based on defined local concentrations of the component proteins. In one mode, signaling occurs while G protein and receptor are unclustered and GAP eliminates signaling; in another, G protein and receptor are clustered and GAP can rapidly modulate signaling but does not eliminate it. Experimental data from multiple GTPase-cycle modules is interpreted in light of these predictions. The latter mode explains previously paradoxical data in which GAP does not alter maximal current amplitude of G protein-activated ion channels, but hastens signaling. The predictions indicate how variations in local concentrations of the component proteins create GTPase-cycle modules with distinctive phenotypes. They provide a quantitative framework for investigating how regulation of local concentrations of components of the GTPase-cycle module affects signaling.
authors.affiliation: Departments of Chemistry and Biochemistry, Cellular and Molecular Medicine, and Bioengineering and San Diego Supercomputer Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Bornheimer
authors.forename: Mano R
authors.initials: MR
authors.lastname: Maurya
authors.forename: Marilyn Gist
authors.initials: MG
authors.lastname: Farquhar
authors.forename: Shankar
authors.initials: S
authors.lastname: Subramaniam
mesh_terms: D020558:GTP Phosphohydrolases / Q000737:chemistry* / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D020690:GTPase-Activating Proteins; D043562:Receptors, G-Protein-Coupled; D020558:GTP Phosphohydrolases; D020962:Heterotrimeric GTP-Binding Proteins