Simple synthesis of deuterium and 13C labeled trifluoromethyl phenyldiazirine derivatives as stable isotope tags for mass spectrometry.
The synthesis of trifluoromethyl diazirine with a stable isotope tag is reported. We found that both Friedel-Crafts acylation and reduction of aryl carbonyl to methylene, using commercially available stable-isotope reagents, were utilized for the synthesis of diazirinyl fatty acid derivatives. The stable isotope labeled diazirine may be valuable for identifying binding sites by mass spectrometry.
Hashimoto
Makoto
M
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. hasimoto@obihiro.ac.jp
Hatanaka
Yasumaru
Y
D001665:Binding Sites; D002247:Carbon Isotopes / Q000378:metabolism; D003903:Deuterium / Q000138:chemical synthesis*; D041961:Deuterium Exchange Measurement; D003978:Diazomethane / Q000138:chemical synthesis*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods
D002247:Carbon Isotopes; D003978:Diazomethane; D003903:Deuterium