Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier.
Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.
Hasegawa
Teruaki
T
Department of Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Fujisawa
Tomohisa
T
Numata
Munenori
M
Matsumoto
Takahiro
T
Umeda
Mariko
M
Karinaga
Ryouji
R
Mizu
Masami
M
Koumoto
Kazuya
K
Kimura
Taro
T
Okumura
Shiro
S
Sakurai
Kazuo
K
Shinkai
Seiji
S
D002452:Cell Count; D002942:Circular Dichroism; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D009844:Oligosaccharides / Q000737:chemistry*; D011061:Poly A / Q000737:chemistry; D011066:Poly C / Q000737:chemistry; D012680:Sensitivity and Specificity; D012566:Sizofiran / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D006027:Glycosides; D046911:Macromolecular Substances; D016376:Oligonucleotides, Antisense; D009844:Oligosaccharides; C008245:lactosides; D011061:Poly A; C015465:poly(dA); D011066:Poly C; D012566:Sizofiran