title: Anti-endostatin monoclonal antibody enhances growth of human hepatocellular carcinoma cells by inhibiting activity of endostatin secreted by the transplanted cells in nude mice.
abstract: Endostatin, a fragment of collagen XVIII, inhibits angiogenesis in tumors, and is expected to become a new anticancer drug. However, its effectiveness is still controversial, because some researchers failed to reproduce the same marked regression of tumors by the peptide. We gave anti-endostatin monoclonal antibody, designated as CH18B, to nude mice transplanted with human hepatocellular carcinoma cells (JHH-1 line) that endogenously produced endostatin from collagen XVIII secreted by the cells themselves. As a result, CH18B promoted tumor angiogenesis by inhibiting endostatin activity in the tumor and subsequently increased tumor mass by preventing cancer cells from undergoing apoptosis. But the antibody itself did not stimulate proliferation of the tumor cells. Our present experimental procedure, the use of anti-endostatin antibody, definitely solved the question whether endostatin might exert its anticancer activity.
authors.affiliation: Department of Hepatology, Shigei Medical Research Hospital, 2117 Yamada, Okayama 701-0202, Japan. tsuboi@shigei.or.jp
authors.forename: So
authors.initials: S
authors.lastname: Tsuboi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nouso
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Tomono
authors.forename: Seishi
authors.initials: S
authors.lastname: Nagamori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Naito
authors.forename: Tsunakazu
authors.initials: T
authors.lastname: Watanabe
authors.forename: Koji
authors.initials: K
authors.lastname: Manabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohmori
authors.forename: Harushige
authors.initials: H
authors.lastname: Nakatsukasa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Higashi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shiratori
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Namba
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Ninomiya
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology* / Q000662:veterinary; D049109:Cell Proliferation; D043169:Endostatins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology* / Q000662:veterinary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental; D009389:Neovascularization, Pathologic; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D043169:Endostatins