title: Identification and characterization of nuclear localization signals of CaMKP-N.
abstract: Calmodulin-dependent protein kinase phosphatase (CaMKP) and CaMKP-N dephosphorylate and regulate multifunctional Ca(2+)/calmodulin-dependent protein kinases. The enzymatic properties of CaMKP-N and CaMKP resemble each other, whereas their localizations are different. CaMKP-N is localized in the nucleus, whereas CaMKP is localized in the cytosol. In the present study, the nuclear localization signals (NLSs) of CaMKP-N were identified and characterized. CaMKP-N contains two NLSs, NLS1 and NLS2, at the C-terminus. A cluster of basic residues in the NLSs is important for their function. NLS1 and NLS2 function independently, but mutagenesis analysis suggests that these NLSs interact with each other.
authors.affiliation: Department of Biochemistry, Asahikawa Medical College, Asahikawa 078-8510. mtake@asahikawa-med.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Takanobu
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Fujisawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D003600:Cytosol / Q000378:metabolism; D017353:Gene Deletion; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D019913:Nuclear Localization Signals; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000378:metabolism; D000071636:Protein Phosphatase 2C; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D019913:Nuclear Localization Signals; D049452:Green Fluorescent Proteins; C110531:calmodulin dependent protein kinase II phosphatase; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C