Exchange transfusion with synthetic oxygen-carrying plasma protein "albumin-heme" into an acute anemia rat model after seventy-percent hemodilution.
Recombinant human serum albumin (rHSA) incorporating the synthetic heme "albumin-heme" is an oxygen-carrying plasma protein that has the potential to be a red blood cell substitute. The physiological responses to a 30% exchange transfusion with two types of albumin-heme (rHSA-FecycP, rHSA-FepivP) solutions after 70% isovolemic hemodilution with 5 g/dL rHSA were investigated using anesthetized rats. The circulation parameters, blood parameters, renal cortical oxygen pressure (pO2), and muscle tissue pO2 were carefully monitored for 60 min after the injection. The declined mean arterial pressure and the mixed venous partial pO2 significantly recovered to 70.8 and 91.9% of the basal values by intravenous infusion of albumin-hemes, respectively. The lowered renal cortical pO2 also increased, indicating oxygen transport by this synthetic hemoprotein. The administration of albumin-heme into the acute anemia rat model after hemorrhage improved the circulatory volume and resuscitated the shock state. Both rHSA-FecycP and rHSA-FepivP transported oxygen through the body.
Komatsu
Teruyuki
T
Advanced Research Institute for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Yamamoto
Hisashi
H
Huang
Yubin
Y
Horinouchi
Hirohisa
H
Kobayashi
Koichi
K
Tsuchida
Eishun
E
D000740:Anemia / Q000188:drug therapy*; D000818:Animals; D001784:Blood Gas Analysis; D001798:Blood Proteins / Q000627:therapeutic use*; D001802:Blood Substitutes / Q000627:therapeutic use*; D005078:Exchange Transfusion, Whole Blood / Q000379:methods*; D006418:Heme / Q000494:pharmacology*; D006438:Hemodilution; D006439:Hemodynamics / Q000187:drug effects; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics / Q000187:drug effects; D012709:Serum Albumin / Q000627:therapeutic use*; D012771:Shock, Hemorrhagic / Q000188:drug therapy / Q000503:physiopathology; D016019:Survival Analysis
D001798:Blood Proteins; D001802:Blood Substitutes; D012709:Serum Albumin; D006418:Heme; D010100:Oxygen