Non-viral delivery of ribozymes for cancer gene therapy.
Ribozymes are RNA molecules with the capacity to effect sequence-specific cleavage of other transcripts. Since their initial discovery, there has been considerable interest in the development of ribozymes and other RNA therapeutics for gene therapy, particularly in the realm of cancer. However, as with other gene therapy applications, the delivery of ribozyme-based therapeutics to the target tissues of interest has represented a significant obstacle to the maturation of this technology to the clinical arena. This review will discuss the progress made so far in the use of non-viral methods for the systemic delivery of ribozymes for cancer gene therapy.
Kashani-Sabet
Mohammed
M
Auerback Melanoma Research Laboratory, Cutaneous Oncology Program, UCSF Comprehensive Cancer Center, Department of Dermatology, University of California San Francisco, CA 94115, USA. kashanim@derm.ucsf.edu
D000818:Animals; D017322:Clinical Trials, Phase II as Topic; D004337:Drug Carriers; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use; D006801:Humans; D008546:Melanoma, Experimental / Q000235:genetics / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics; D009362:Neoplasm Metastasis / Q000188:drug therapy; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D009360:Neoplastic Cells, Circulating; D010957:Plasmids / Q000235:genetics / Q000627:therapeutic use; D016337:RNA, Catalytic / Q000008:administration & dosage / Q000627:therapeutic use*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000037:antagonists & inhibitors / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
C121301:Angiozyme; D004337:Drug Carriers; D019309:Epstein-Barr Virus Nuclear Antigens; D016328:NF-kappa B; D016337:RNA, Catalytic; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1; C417029:EBV-encoded nuclear antigen 1