title: The VP6 protein of rotavirus interacts with a large fraction of human naive B cells via surface immunoglobulins.
abstract: Immunity to human group A rotavirus (RV), a major cause of viral gastroenteritis in infants, involves B lymphocytes that provide RV-specific antibodies. Additionally, some arguments suggest that naive B cells could be implicated in the first steps of the immune response against RV. The aim of our study was to analyze the interaction of VP6 and VP7 RV capsid proteins with human B cells depending on the immune status of the individual, i.e., naive or RV experienced. For this purpose, a two-color virus-like particle flow cytometry assay was devised to evaluate the blood B-lymphocyte reactivity to VP6 and VP7 proteins from healthy RV-exposed adults, recently infected infants, and neonates at birth. Both VP6 and VP7 interactions with B cells were mediated by surface immunoglobulins and probably by their Fab portions. VP7-reactive B lymphocytes were mainly detected from RV-experienced patients and almost exclusively in the CD27-positive memory cell fraction. Conversely, VP6-reactive B lymphocytes were detected at similar and high frequencies in adult, infant, and neonate samples. In adult samples, VP6 reacted with about 2% of the CD27-negative (CD27(neg)) naive B cells. These results demonstrated that the VP6 RV protein interacted with a large fraction of naive B lymphocytes from both adults and neonates. We propose that naive B cell-VP6 interaction might influence the strength and quality of the acquired immune response and should be considered for elaborating RV vaccine strategies.
authors.affiliation: Service des Urgences Médicales Pédiatriques, Hôpital Armand Trousseau, 26 ave. du Dr. Arnold Netter, 75571 Paris cedex 12, France. nathalie.parez@trs.ap-hop-paris.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Parez
authors.forename: Antoine
authors.initials: A
authors.lastname: Garbarg-Chenon
authors.forename: Cynthia
authors.initials: C
authors.lastname: Fourgeux
authors.forename: Françoise
authors.initials: F
authors.lastname: Le Deist
authors.forename: Annabelle
authors.initials: A
authors.lastname: Servant-Delmas
authors.forename: Annie
authors.initials: A
authors.lastname: Charpilienne
authors.forename: Jean
authors.initials: J
authors.lastname: Cohen
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D022243:Rotavirus Vaccines / Q000276:immunology; D014771:Virion / Q000502:physiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D011947:Receptors, Antigen, B-Cell; D022243:Rotavirus Vaccines; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus