title: Serum E-selectin and erythrocyte membrane Na+K+ ATPase levels in patients with rheumatoid arthritis.
abstract: We conducted this study to assess serum soluble E-selectin (sE-selectin) levels and erythrocyte membrane Na(+)K(+) ATPase activity in patients with rheumatoid arthritis (RA) and correlate the levels with disease activity. Levels of sE-selectin were measured in the serum of 20 patients with RA and 20 control subjects by an enzyme-linked immunosorbant assay. Na(+)K(+) ATPase activity was determined by a colorimetric method in RA patients and healthy controls. There were no statistically significant differences between the two groups with respect to demographic data such as age and sex (p > 0.05). The serum levels of sE-selectin, ESR and C-reactive protein (CRP) in RA patients were significantly higher than in healthy controls (p < 0.001). Erythrocyte membrane Na(+)K(+) ATPase activity was significantly lower in the RA group than in the control group (p < 0.001). Correlation analysis revealed significant positive correlations between soluble E-selectin and ESR (r = 0.457; p < 0.05) and CRP (r = 0.682; p < 0.01) levels. There were statistically significant negative correlations between erythrocyte membrane Na(+)K(+) ATPase activity and ESR (r = -0.450; p < 0.05) and CRP (r = -0.446; p < 0.05) levels. Additionally, a significant negative correlations between sE-selectin and Na(+)K(+) ATPase activity was observed (r = -0.80; p < 0.001). These results show that decreases in erythrocyte membrane Na(+)K(+) ATPase activity and increases in sE-selectin are observed in RA, and that increased levels of sE-selectin may also reflect disease status or activity.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Medical Faculty, Atatürk University, Erzurum, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Yildirim
authors.forename: Kazim
authors.initials: K
authors.lastname: Senel
authors.forename: Saliha
authors.initials: S
authors.lastname: Karatay
authors.forename: Meltem
authors.initials: M
authors.lastname: Sisecioglu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kiziltunc
authors.forename: Mahir
authors.initials: M
authors.lastname: Ugur
authors.forename: Fatih
authors.initials: F
authors.lastname: Akcay
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D019040:E-Selectin / Q000097:blood*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D019040:E-Selectin; D002097:C-Reactive Protein; D000254:Sodium-Potassium-Exchanging ATPase