Nonisotopic, semiautomated plasmodium falciparum bioassay for measurement of antimalarial drug levels in serum or plasma.
A simple, nonisotopic, semiautomated bioassay for the measurement of antimalarial drug levels in plasma or serum based on the quantitation of histidine-rich protein II in malaria culture is presented. The assay requires only small sample volumes and was found to be highly sensitive and reproducible. The results closely paralleled those obtained with isotopic bioassays (R = 0.988, P < 0.001) and high-performance liquid chromatography-electrochemical detection (R = 0.978, P < 0.001).
Noedl
Harald
H
Department of Specific Prophylaxis and Tropical Medicine, Institute of Pathophysiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria. harald.noedl@univie.ac.at
Teja-Isavadharm
Paktiya
P
Miller
Robert Scott
RS
D000818:Animals; D000962:Antimalarials / Q000097:blood* / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology; D000077332:Artesunate; D001331:Automation; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D010963:Plasmodium falciparum / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology; D000099053:Histidine-Rich Glycoprotein
D000962:Antimalarials; D037621:Artemisinins; D011506:Proteins; D012717:Sesquiterpenes; D000099053:Histidine-Rich Glycoprotein; D000077332:Artesunate