title: Epidermal cell analysis by RT-PCR.
abstract: Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.
authors.affiliation: Ottawa Health Research Institute, Ottawa, Ontario, Canada.
authors.forename: Tammy-Claire
authors.initials: TC
authors.lastname: Troy
authors.forename: Robert Man-Kit
authors.initials: RM
authors.lastname: Cheung
authors.forename: Kursad
authors.initials: K
authors.lastname: Turksen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012333:RNA, Messenger