title: Fully-automated chip-based nanoelectrospray tandem mass spectrometry of gangliosides from human cerebellum.
abstract: The introduction of chip-based electrospray (ESI) ion sources into biological mass spectrometry (MS) addressed the fundamental issue of how to analyze minute amounts of complex biological systems. The automation of sample delivery into the MS combined with the chip-based ESI allows for high quality bioanalysis in a high-throughput fashion. These advantages have already been demonstrated in proteomics, direct screening of drugs and drug discovery. As part of our continuing effort to implement automated chip-based mass spectrometry into the field of complex carbohydrate analysis, we hereby report the development of a chipESI MS and MS/MS methodology for the screening of gangliosides. A strategy to characterize a complex ganglioside mixture from human cerebellar tissue, by automated ESIchip-quadrupole time-of-flight (QTOF) MS and MS/MS is presented here. The feasibility of this method, and the general experimental requirements for automated chipESI MS analysis of these carbohydrate species is described.
authors.affiliation: Institute for Medical Physics and Biophysics, Biomedical Analysis, University of Münster, Münster, Germany. zamfir@uni-muenster.de
authors.forename: Alina
authors.initials: A
authors.lastname: Zamfir
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Vukelić
authors.forename: Laura
authors.initials: L
authors.lastname: Bindila
authors.forename: Jasna
authors.initials: J
authors.lastname: Peter-Katalinić
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Almeida
authors.forename: Alistair
authors.initials: A
authors.lastname: Sterling
authors.forename: Mark
authors.initials: M
authors.lastname: Allen
mesh_terms: D000328:Adult; D002531:Cerebellum / Q000737:chemistry*; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D046208:Microchip Analytical Procedures; D036103:Nanotechnology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D005732:Gangliosides