Alterations of mast cell mediator production and release by gamma-linolenic and docosahexaenoic acid.
The purpose of our investigations was to evaluate the supposed beneficial effects of gamma-linolenic (GLA) and docosahexaenoic acid (DHA) in a canine mastocytoma cell line (C2) as a model for canine atopic dermatitis. Cells were cultured in a basic medium (DEH) and in DEH supplemented with 14.3 microM GLA (DEH-GLA) or 14.3 microM DHA (DEH-DHA) for 8 days. Chymase and tryptase activity, as well as histamine and prostaglandin (PG)E(2) release were measured. To stimulate histamine and PGE(2) release, cells were incubated with the wasp venom peptide mastoparan (50 microM) for 30 min. GLA increased tryptase activity and decreased histamine release after C2 stimulation. DHA diminished PGE(2) production in activated C2. These results support the prescription of GLA- and DHA-enriched diets to reduce inflammatory signs in canine atopic dermatitis.
Gueck
Thomas
T
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany. gueck@vetmed.uni-leipzig.de
Seidel
Anja
A
Baumann
Daniela
D
Meister
Antje
A
Fuhrmann
Herbert
H
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D053818:Chymases; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000662:veterinary*; D019587:Dietary Supplements; D015232:Dinoprostone / Q000096:biosynthesis; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D006632:Histamine / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D012697:Serine Endopeptidases / Q000096:biosynthesis; D053802:Tryptases; D017965:gamma-Linolenic Acid / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D018836:Inflammation Mediators; D004281:Docosahexaenoic Acids; D017965:gamma-Linolenic Acid; D006632:Histamine; D012697:Serine Endopeptidases; D053818:Chymases; D053802:Tryptases; D015232:Dinoprostone