GPCR-interacting proteins (GIPs): nature and functions.
The simplistic idea that seven transmembrane receptors are single monomeric proteins that interact with heterotrimeric G-proteins after agonist binding is definitively out of date. Indeed, GPCRs (G-protein-coupled receptors) are part of multiprotein networks organized around scaffolding proteins. These GIPs (GPCR-interacting proteins) are either transmembrane or cytosolic proteins. Proteomic approaches can be used to get global pictures of these 'receptosomes'. This approach allowed us to identify direct but also indirect binding partners of serotonin receptors. GIPs are involved in a wide range of functions including control of the targeting, trafficking and signalling of GPCRs. One of them, Shank, which is a secondary and tertiary partner of metabotropic and ionotropic glutamate receptors, respectively, can induce the formation of a whole functional glutamate 'receptosome' and the structure to which it is associated, the dendritic spine.
Bockaert
J
J
LGF, UPR CNRS 2580, 141 rue de la Cardonille, 34094 Montpellier, Cedex 5, France.
Roussignol
G
G
Bécamel
C
C
Gavarini
S
S
Joubert
L
L
Dumuis
A
A
Fagni
L
L
Marin
P
P
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009419:Nerve Tissue Proteins; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D040901:Proteomics; D018091:Receptors, AMPA / Q000737:chemistry; D011985:Receptors, Serotonin / Q000737:chemistry; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism; D011506:Proteins
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D018091:Receptors, AMPA; D011985:Receptors, Serotonin; C120531:Shank1 protein, rat; C512356:C1QTNF1 protein, human; D011506:Proteins