title: Redox status of the eye lens: a regional study.
abstract: The aim of this work was to study the regional variation of some antioxidant systems in calf lens. Specific lens regions of nearly same age were obtained by a microsectioning technique, and the concentration of reduced and oxidized glutathione, protein sulfhydryl groups, and iron were measured in each lens region. The concentration of reduced glutathione, the major redox buffer in lens, exponentially decreased from the cortical regions to the nucleus. In contrast, the concentration of protein sulfhydryl groups gradually increased from the cortex toward the nucleus. The protein-bound disulfides remained constant throughout the lens. Iron was concentrated in the outer cortical region. The results show that the most dynamic redox-active zone in the lens is the subcapsular cortical region where the oxidant flux meets a highly reducing environment containing a potent redox catalyst.
authors.affiliation: Department of Experimental Ophthalmology, University of Bonn, Abbéstr. 2, 53105 Bonn, Germany. margirova@hotmail.com
authors.forename: Mariana
authors.initials: M
authors.lastname: Argirova
authors.forename: Mischa
authors.initials: M
authors.lastname: Kleine-Reidick
authors.forename: Winrich
authors.initials: W
authors.lastname: Breipohl
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods; D002386:Cataract / Q000378:metabolism; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D007908:Lens, Crystalline / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry; D017382:Reactive Oxygen Species
chemical_list: D004220:Disulfides; D011506:Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D007501:Iron; D005978:Glutathione; D010100:Oxygen