title: High-performance liquid chromatography-coordination ion spray-mass spectrometry (HPLC-CIS/MS): a new tool for the analysis of non-polar compound classes in plant extracts using the example of Piper methysticum Forst.
abstract: An efficient method to characterise complex plant extracts is described using the example of Piper methysticum Forst. (kava; Piperaceae). The method is based on the on-line coupling of high-performance liquid chromatography to a new detection technique: coordination ion spray-mass spectrometry (CIS/MS). CIS/MS is a universal, novel ionisation technique improving selectivity as well as sensitivity. Charged complexes were formed through addition of central complexing ions such as sodium, silver and cobalt. The advantages of CIS/MS detection compared with the electrospray ionisation detection are discussed. The experimental set-up and the application of this simple and robust technique is described to show the its various fields of application in the analysis of plant extracts.
authors.affiliation: Institute of Pharmacy, Department of Pharmaceutical Analysis, University of Tübingen, Auf der Morgenstelle 8, D-72076 Tübingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gaub
authors.forename: A
authors.initials: A
authors.lastname: von Brocke
authors.forename: G
authors.initials: G
authors.lastname: Roos
authors.forename: K A
authors.initials: KA
authors.lastname: Kovar
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020901:Kava / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D011753:Pyrones / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D011753:Pyrones