Effects of nerve growth factor on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium cells.
OBJECTIVE
To investigate the effects of nerve growth factor(NGF) on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium(RPE)cells in vitro.


METHODS
Primary culture and subculture of human fetal retinal pigment epithelium cells were established in vitro first. Cultured RPE cells were treated with NGF by various concentrations 0 micro1g/L, 50 microg/L, 100 microg/L, 200 microg/L and 300 microg/L(final concentration) for 48 hs. After 48 hs, cells proliferation was measured with methyl thiazolyl tetrazolium (MTT) assay method and the amount of DNA was determined by the absorbance at 280 nm of nucleic acid & protein analysis.


RESULTS
The A values of 100 microg/L, 200 microg/L, 300 microg/L NGF was(0.2137 +/- 0.233), (0.2188 +/- 0.0181), (0.2322 +/- 0.0164) as compared with(0.1897 +/- 0.0152) of A value of 0 microg/L NGF respectively, q value was 3.63, 4.40, 6.42 and P value was 0.015, 0.000, 0.000( q-test). The DNA concentrations of 100 microg/L, 200 microg/L, 300 microg/L and 400 microg/L NGF was (981. 220 4 +/- 123. 535 7), (1 375. 848 4 +/- 244. 471 8), (1 658. 707 1 176. 938 1), (2 353. 086 3 +/- 609. 906 4) microg/ml as compared with (666. 818 8 +/- 141. 330 2) microg/ml of DNA concentration of 0 microg/L NGF respectively, q value was 3. 63,8. 20,11. 47,19. 46, P value was 0. 024,0. 000,0. 000,0. 000(q-test).


CONCLUSIONS
The data suggested that NGF could stimulate the proliferation and DNA synthesis of cultured of hRPE cells in vitro in a dose-dependent manner.
Li
Wensheng
W
Department of Ophthalmology, the Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China.
Wen
Jun
J
Jiang
Deyong
D
Ding
Jianguang
J
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D006801:Humans; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000378:metabolism
D004247:DNA; D020932:Nerve Growth Factor