Leptin kinetics during peritoneal dialysis in acutely uraemic rats.
BACKGROUND
Leptin has been shown to function as an inhibitor of appetite and energy expenditure accelerator. However, it was recently reported that leptin has other important functions as a fibrogenetic factor and a novel, independent risk factor for coronary heart disease. The present study aimed to assess the blood concentration of leptin in acute uraemic rats by using various peritoneal dialysis (PD) solutions.


METHODS
To induce acute renal failure, the bilateral renal arteries were ligated via a mid-abdominal incision 1 h before starting PD. Rats were divided into four groups: 13.6 g/L glucose-containing dialysate (group L); 38.6 g/L glucose-containing dialysate (group H); 13.6 g/L glucose and 25 g/L mannitol-containing dialysate with equal osmotic pressure to the dialysate of group H (group M); and renal failure without PD (group F). The concentrations of glucose, urea nitrogen (UN), leptin and insulin were measured at 0, 2 and 4 h after starting PD.


RESULTS
We observed significant blood UN suppression in all dialysed groups. Blood glucose was significantly higher in rats treated with the high glucose solution than in those treated with the low glucose solution. Insulin and leptin significantly increased in the high glucose solution group. There was a strong correlation between the blood glucose and insulin levels. We also found a strong correlation between the percentage changes in blood glucose and leptin. The relationship between the percentage changes in insulin and leptin were weak but significant.


CONCLUSIONS
The high glucose PD solution resulted in increased circulating levels of leptin, glucose, and insulin, suggesting that these changes are linked with PD performed with glucose-based dialysis fluid.
Matsubara
Keisuke
K
The Second Department of Internal Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan.
Kiyomoto
Hideyasu
H
Moriwaki
Kumiko
K
Hara
Taiga
T
Kondo
Naoki
N
Shokoji
Takatomi
T
Hitomi
Hirofumi
H
Aki
Yasuharu
Y
Aono
Masaki
M
Nishiyama
Akira
A
Ohmori
Koji
K
Kohno
Masakazu
M
D058186:Acute Kidney Injury / Q000097:blood* / Q000150:complications; D000818:Animals; D005950:Glucose Solution, Hypertonic / Q000494:pharmacology*; D015312:Hemodialysis Solutions / Q000494:pharmacology*; D020738:Leptin / Q000097:blood*; D008297:Male; D010530:Peritoneal Dialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014511:Uremia / Q000097:blood* / Q000209:etiology
D005950:Glucose Solution, Hypertonic; D015312:Hemodialysis Solutions; D020738:Leptin