title: Estrogen acidifies vaginal pH by up-regulation of proton secretion via the apical membrane of vaginal-ectocervical epithelial cells.
abstract: The objective of this study was to assess estrogen-dependent cellular mechanisms that could contribute to the acid pH of the vaginal lumen. Cultures of normal human cervical-vaginal epithelial (hECE) cells and endocervical cells were grown on filters, and acidification of the extracellular solutions on the luminal (L-pHo) and contraluminal (CL-pHo) sides was measured. The hECE cells and endocervical cells decreased CL-pHo from 7.40 to 7.25 within 20-30 min of incubation in basic salt solution. Endocervical cells also produced a similar decrease in L-pHo. In contrast, hECE cells acidified L-pHo down to pH 7.05 when grown as monoculture and down to pH 6.05 when grown in coculture with human cervical fibroblasts. This enhanced acid secretion into the luminal compartment was estrogen dependent because removal of endogenous steroid hormones attenuated the effect, whereas treatment with 17beta-estradiol restored it. The 17beta-estradiol effect was dose dependent (EC50 0.5 nm) and could be mimicked by diethylstilbestrol and in part by estrone and tamoxifen. Preincubation with ICI-182780, but not with progesterone, blocked the estrogen effect. Preincubation of cells with the V-ATPase blocker bafilomycin A1, when administered to the luminal solution, attenuated the baseline and estrogen-dependent acid secretion into the luminal solution. Treatment with EGTA, to abrogate the tight junctional resistance, blocked the decrease in L-pHo and stimulated a decrease in CL-pHo, indicating that the tight junctions are necessary for maintaining luminal acidification. We conclude that vaginal-ectocervical cells acidify the luminal canal by a mechanism of active proton secretion, driven in part by V-H+-ATPase located in the apical plasma membrane and that the baseline active net proton secretion occurs constitutively throughout life and that this acidification is up-regulated by estrogen.
authors.affiliation: Department of Reproductive Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA. gig@cwru.edu
authors.forename: George I
authors.initials: GI
authors.lastname: Gorodeski
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hopfer
authors.forename: Chung Chiun
authors.initials: CC
authors.lastname: Liu
authors.forename: Ellen
authors.initials: E
authors.lastname: Margles
mesh_terms: D000143:Acids / Q000378:metabolism*; D000328:Adult; D002118:Calcium / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology*; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008875:Middle Aged; D017697:Premenopause; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011522:Protons; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology; D019108:Tight Junctions / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014621:Vagina / Q000166:cytology*
chemical_list: D000143:Acids; D004967:Estrogens; D011522:Protons; D020845:Selective Estrogen Receptor Modulators; D006180:Proton-Translocating ATPases; D002118:Calcium