Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation.
A substantial number of G-protein-coupled receptor-interacting proteins have been identified initially by the use of yeast two-hybrid screens. Using the C-terminal tail of both opioid receptors and the melanin concentrating hormone receptor-1 as bait, the actin and intermediate filament-binding protein periplakin was isolated. In each case, the site of interaction is within helix VIII of the receptor and periplakin limits agonist-mediated G-protein activation potentially by competing with G-protein for this region of the receptor.
Milligan
G
G
Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Davidson Building, Glasgow G12 8QQ, Scotland, UK. g.milligan@bio.gla.ac.uk
Murdoch
H
H
Kellett
E
E
White
J H
JH
Feng
G-J
GJ
D000494:Allosteric Regulation; D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006801:Humans; D051179:Plakins; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism; D044101:Receptors, Melanocortin / Q000737:chemistry; D020798:Two-Hybrid System Techniques
D003598:Cytoskeletal Proteins; D051179:Plakins; C110037:Ppl protein, mouse; D043562:Receptors, G-Protein-Coupled; D044101:Receptors, Melanocortin; D019204:GTP-Binding Proteins