Isolation of the repertoire of VSG expression site containing telomeres of Trypanosoma brucei 427 using transformation-associated recombination in yeast.
Trypanosoma brucei switches between variant surface glycoproteins (VSGs) allowing immune escape. The active VSG is in one of many telomeric bloodstream form VSG expression sites (BESs), also containing expression site-associated genes (ESAGs) involved in host adaptation. The role of BES sequence diversity in parasite virulence can best be understood through analysis of the full repertoire of BESs from a given T. brucei strain. However, few BESs have been cloned, as telomeres are highly underrepresented in standard libraries. We devised a strategy for isolating the repertoire of T. brucei 427 BES-containing telomeres in Saccaromyces cerevisiae by using transformation-associated recombination (TAR). We isolated 182 T. brucei 427 BES TAR clones, 167 of which could be subdivided into minimally 17 BES groups. This set gives us the first view of the breadth and diversity of BESs from one T. brucei strain. Most BESs ranged between 40 and 70 kb (average, 57 +/- 17 kb) and contained most identified ESAGs. Phylogenetic comparison of the cohort of BES promoter and ESAG6 sequences did not show similar trees, indicating rapid evolution most likely mediated by sequence exchange between BESs. This cloning strategy could be used for any T. brucei strain, facilitating research on the biodiversity of telomeric gene families and host-pathogen interactions.
Becker
Marion
M
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Aitcheson
Niall
N
Byles
Elaine
E
Wickstead
Bill
B
Louis
Edward
E
Rudenko
Gloria
G
D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000472:pathogenicity*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D014643:Variant Surface Glycoproteins, Trypanosoma