title: PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.
abstract: The cDNA for PRMT7, a recently discovered human protein-arginine methyltransferase (PRMT), was cloned and expressed in Escherichia coli and mammalian cells. Immunopurified PRMT7 actively methylated histones, myelin basic protein, a fragment of human fibrillarin (GAR) and spliceosomal protein SmB. Amino acid analysis showed that the modifications produced were predominantly monomethylarginine and symmetric dimethylarginine (SDMA). Examination of PRMT7 expressed in E. coli demonstrated that peptides corresponding to sequences contained in histone H4, myelin basic protein, and SmD3 were methylated. Furthermore, analysis of the methylated proteins showed that symmetric dimethylarginine and relatively small amounts of monomethylarginine and asymmetric dimethylarginine were produced. SDMA was also formed when a GRG tripeptide was methylated by PRMT7, indicating that a GRG motif is by itself sufficient for symmetric dimethylation to occur. Symmetric dimethylation is reduced dramatically compared with monomethylation as the concentration of the substrate is increased. The data demonstrate that PRMT7 (like PRMT5) is a Type II methyltransferase capable of producing SDMA modifications in proteins.
authors.affiliation: University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Molecular Genetics, Microbiology and Immunology, Piscataway, New Jersey 08854, USA.
authors.forename: Jin-Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeffry R
authors.initials: JR
authors.lastname: Cook
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Olga
authors.initials: O
authors.lastname: Mirochnitchenko
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Gunderson
authors.forename: Arthur M
authors.initials: AM
authors.lastname: Felix
authors.forename: Nicole
authors.initials: N
authors.lastname: Herth
authors.forename: Ralf
authors.initials: R
authors.lastname: Hoffmann
authors.forename: Sidney
authors.initials: S
authors.lastname: Pestka
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D004926:Escherichia coli; D006367:HeLa Cells; D006801:Humans; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011484:Protein-Arginine N-Methyltransferases
chemical_list: C487735:dimethylarginine; D001120:Arginine; D008780:Methyltransferases; C487994:PRMT7 protein, human; D011484:Protein-Arginine N-Methyltransferases