title: [The heat shock protein 70 antisense oligomers enhance the sensitivity of bladder cancer cell EJ to mitomycin C].
abstract: OBJECTIVE
  To investigate whether the heat shock protein (HSP) 70 antisense oligomers can enhance the sensitivity of bladder cancer cell EJ to mitomycin C.
  
  
  METHODS
  The HSP70 mRNA of EJ cells was blocked by the 10 micromol/L HSP70 antisense oligomers, while its effect on cell growth was evaluated by methyl thiazolyl tetrazolium (MTT) and colony forming ability test.
  
  
  RESULTS
  The HSP70 expressions in HSP70 antisense treated group were lower than the corresponding sense and nonsense treated groups (P < 0.01). While, the increased sensitivity of EJ to mitomycin C was found in antisense treated group, compared with the corresponding sense and nonsense treated groups (P < 0.01).
  
  
  CONCLUSIONS
  The sensitivity of bladder cancer cell EJ to mitomycin C was enhanced by the blockage of the HSP70 expression.
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Ling-feng
authors.initials: LF
authors.lastname: He
authors.forename: Shu-kun
authors.initials: SK
authors.lastname: Hou
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yan
authors.forename: Liang
authors.initials: L
authors.lastname: Ren
authors.forename: Shen-wu
authors.initials: SW
authors.lastname: Wang
mesh_terms: D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016685:Mitomycin