[Evaluation of water-soluble vitamins in the vitamin preparation Spofavit dragée using reversed-phase HPLC].
A reversed-phase HPLC gradient method with good separation efficiency was developed and validated for the evaluation of water-soluble vitamins in the pharmaceutical preparation Spofavit dragee. Analyses were performed on a C8 column (LiChrospher RP-select B), the mobile phase was a mixture of phosphate buffer with sodium hexanesulphonate and acetonitrile. Detection was at 280 and 210 nm. The method is capable within 7 minutes to evaluate 6 water-soluble vitamins (ascorbic acid, nicotinamide, panthotenate.Ca, pyridoxine.HCl, thiamine.HCl, and riboflavin). All validated parameters of this method are discussed; the developed method appeared robust and suitable for evaluation of water-soluble vitamins in vitamin preparations.
Jedlicka
A
A
Zentiva - Léciva a.s., Praha. ales.jedlicka@zentiva.cz
Klimes
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D012995:Solubility; D013607:Tablets; D014815:Vitamins / Q000032:analysis*
D013607:Tablets; D014815:Vitamins