[Study on relationship between leukocytes and early diabetic retinopathy].
OBJECTIVE
To explore the leukocytic functions in adhesion, stacking and causing microcirculatory disorder in retinal microvessels in early diabetic rats.


METHODS
Sixteen healthy adult male Wistar rats were randomly divided into normal control group and 3 months group of diabetes (STZ) . We carried out morphological observations and CD45 (Leukocyte Common Antigen) monoclonal antibody immunohistochemical studies of the retinal digest preparations.


RESULTS
Morphological changes including capillary varix, irregularity of capillary caliber, was found in the retinal blood capillaries in diabetic rats at 3 months. Further more, the expression of CD45 was significantly increased in the diabetic retinal microvessels, which were found leukocytes adhering and stacking.


CONCLUSIONS
Changes of retinal microvessels can be found in STZ diabetic rats at 3 months. The leukocytes were important in the onset and development of diabetic retinopathy (DR).
Bai
Ningyan
N
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Tang
Shibo
S
Lin
Jianxian
J
Zhu
Banghao
B
Luo
Yan
Y
Lin
Shaofen
S
D000818:Animals; D002448:Cell Adhesion; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D003930:Diabetic Retinopathy / Q000473:pathology*; D017493:Leukocyte Common Antigens; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012171:Retinal Vessels / Q000473:pathology*
D017493:Leukocyte Common Antigens