Genotoxicity evaluation of Isaria sinclairii (ISE) extract.
The mutagenic potential Isaria sinclairii, a traditional Chinese medicine composed of the fruiting bodies of I. sinclairii and its parasitic host larva, was evaluated using short-term genotoxicity tests, namely, the Ames test, chromosome aberration (CA), and micronuclei (MN) tests. In a Salmonella typhimurium assay, I. sinclairii extract (ISE) did not produce any mutagenic response in the absence or presence of 59 mix with TA98, TA100, TA1535, and TA1537. In the chromosome aberration (CA) test, ISE induced no significant effect on Chinese hamster ovary (CHO) cells compared with control. In the MN test, no significant change in the occurrence of micronucleated polychromatic erythrocytes was observed in male ICR mice intraperitoneally administered ISE at doses of 15, 150, or 1500 mg/kg. These results indicate that ISE has no mutagenic potential in these in vitro and in vivo systems.
Ahn
Mi Young
MY
Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
Ryu
Kang Sun
KS
Jee
Sang Duck
SD
Kim
Iksoo
I
Kim
Jin Won
JW
Kim
Yeong Shik
YS
Kim
Hyung Sik
HS
Kim
In Sun
IS
Kang
Se C
SC
Koo
Hyun Jung
HJ
Park
Yo An
YA
Choi
Sul Min
SM
Yoo
Eun Jeong
EJ
Kwack
Seung Jun
SJ
Yoo
Sun Dong
SD
Lee
Byung Mu
BM
D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D016466:CHO Cells; D002869:Chromosome Aberrations / Q000139:chemically induced*; D006224:Cricetinae; D003412:Cricetulus; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D004912:Erythrocytes; D015162:Micronucleus Tests; D009152:Mutagenicity Tests
D004365:Drugs, Chinese Herbal