Enhanced expression of transforming growth factor-beta1 in inflammatory cells and secretory granules in Paneth cells in the small intestine of mice infected with Toxocara canis.
The small intestine is the initial organ which Toxocara canis larvae invade. Information on intestinal pathological changes associated with transforming growth factor-beta1 (TGF-beta1) and secretory granules (SG) in Paneth cells (PCs) caused by T. canis is unclear. Mice orally inoculated with 250 T. canis infective eggs were evaluated by pathological and immunohistochemical assessments with a 294-day investigation. Pathologically, the inflammatory reactions with or without trapped larvae in the submucosa were observed only within the first 28 days post-infection (DPI), with inflammatory injury ranging from severe during 2 DPI to mild between 7 and 28 DPI. The crypts of Leiberkuhn were major larval penetration sites. Enhanced expression of SG in PCs appeared earlier than those of TGF-beta1 in infiltrating cells. The significance of both effectors might be related to the host's defense against larval invasion in the intestinal phase of toxocaral infection.
Fan
Chia-Kwung
CK
Department of Parasitology, College of Medicine, Taipei Medical University, No. 250 Wu-Hsin Street, 110 Taipei, Taiwan, Republic of China. tedfan@tmu.edu.tw
Hung
Chien-Ching
CC
Lin
Yun-Ho
YH
Li
Ming-Hsien
MH
Su
Kua-Eyre
KE
D000818:Animals; D005260:Female; D007249:Inflammation / Q000469:parasitology / Q000473:pathology*; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000473:pathology; D007421:Intestine, Small / Q000166:cytology / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D019879:Paneth Cells / Q000166:cytology / Q000469:parasitology* / Q000473:pathology; D022142:Secretory Vesicles / Q000378:metabolism*; D017166:Toxocara canis / Q000254:growth & development / Q000472:pathogenicity*; D014120:Toxocariasis / Q000469:parasitology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation
C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1