title: Mu and delta opioid-stimulated [35S]GTP gamma S binding in brain and spinal cord of polyarthritic rats.
abstract: Polyarthritis induced by inoculation with complete Freund's adjuvant alters opioid peptides, but does not affect opioid receptor binding. This study was conducted to measure mu and delta opioid receptor-stimulated G-protein activity in brain and spinal cord of rats 19 days after injection of complete Freund's adjuvant or vehicle. Mu and delta opioid-stimulated [35S]GTPgammaS binding measured autoradiographically in caudate-putamen, medial thalamus and periaqueductal gray was unchanged in polyarthritic rats. Delta opioid-stimulated [35S]GTPgammaS binding was significantly decreased in the spinal cord of polyarthritic rats, whereas mu opioid-stimulated activity was unchanged. These data reveal that the functional activity of delta opioid receptors in the spinal cord is altered in polyarthritis.
authors.affiliation: Department of Pharmacology and Toxicology, Virginia Commonwealth University MCV Campus, Richmond, VA 23298, USA.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Cichewicz
authors.forename: Melinda L
authors.initials: ML
authors.lastname: Cox
authors.forename: Sandra P
authors.initials: SP
authors.lastname: Welch
authors.forename: Dana E
authors.initials: DE
authors.lastname: Selley
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Sim-Selley
mesh_terms: D000818:Animals; D001168:Arthritis / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism*; D008297:Male; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D013462:Sulfur Radioisotopes / Q000378:metabolism
chemical_list: D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D013462:Sulfur Radioisotopes; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D016244:Guanosine 5'-O-(3-Thiotriphosphate)