title: Two-photon analysis of calcium signals in T lymphocytes of intact lamina propria from human intestine.
abstract: Lamina propria (LP) T cells of the human intestinal mucosa usually do not develop systemic immune responses despite permanent exposure to foreign antigens. The mechanisms maintaining this hyporeactivity in the normal gut are poorly understood. It is, at present, not clear what role the microenvironment of the mucosa plays for low T cell reactivity and in the pathogenesis of mucosal inflammation. Despite the importance of cytosolic Ca(2+) signals for T lymphocyte activation, intracellular Ca(2+) concentration measurements have so far only been performed in dissociated T cells, following disruption of the microenvironment. We used two-photon technology to measure Ca(2+) signals in identified T lymphocytes within the intact mucosa to minimize impact on tissue integrity while preserving the cellular microenvironment. We show that Ca(2+) signals in LP T cells correlate with the hyporeactivity of T cells in the intestinal immune system and furthermore link Ca(2+) signals with inflammatory bowel disease. Our data implicate that Ca(2+) signals in LP T cells do not depend on the microenvironment of the intact mucosa, since they are very similar to Ca(2+) signals in dissociated LP T cells.
authors.affiliation: Department of Physiology, University of the Saarland, Homburg, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Tutsch
authors.forename: Désirée
authors.initials: D
authors.lastname: Griesemer
authors.forename: Alexander
authors.initials: A
authors.lastname: Schwarz
authors.forename: Andreas
authors.initials: A
authors.lastname: Stallmach
authors.forename: Markus
authors.initials: M
authors.lastname: Hoth
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D003106:Colon / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D002118:Calcium