[Study on the formaldehyde-induced DNA damage with comet assay].
Formaldehyde is known as a genotoxic substance. Numerous studies have shown that formaldehyde could induce DNA-DNA and DNA-protein crosslinks. However, scholars have disagreed with each other on the formaldehyde-induced DNA strand breaks. We chose buccal cells as materials to evaluate the genotoxicity of formaldehyde with comet assay--especially for DNA strand breaks. The results showed that formaldehyde of low concentration induced DNA strand breaks, while formaldehyde of high concentration induced DNA-DNA and DNA-protein crosslinks. According to our experimental results, we proposed the concept of the "peak point of break" at which formaldehyde induced DNA strand breaks most.
Li
Rui
R
College of the Life Sciences, Central China Normal University, Wuhan 430079.
Lu
Zhi Song
ZS
Qiao
Yan
Y
Yao
Han Chao
HC
Yu
Fei Fei
FF
Yang
Xu
X
D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D006801:Humans
D005404:Fixatives; D005557:Formaldehyde