A mass spectrometric investigation of non-covalent interactions between ruthenium complexes and DNA.
Electrospray ionisation mass spectrometry was used to investigate reactions between six ruthenium compounds and three different non self-complementary duplex oligonucleotides containing 16 base pairs. Each of the compounds studied formed non-covalent complexes containing between one and five ruthenium molecules bound to DNA. Competition experiments involving duplex 16mers and pairs of ruthenium compounds were used to determine the order of relative binding affinities of the metal compounds. Other competition experiments involving ruthenium compounds, and the organic DNA binding agents daunomycin and distamycin, provided information about the sites and modes of DNA binding of the ruthenium compounds.
Urathamakul
Thitima
T
Department of Chemistry, University of Wollongong, Northfields Avenue, New South Wales, Australia.
Beck
Jennifer L
JL
Sheil
Margaret M
MM
Aldrich-Wright
Janice R
JR
Ralph
Stephen F
SF
D004247:DNA / Q000737:chemistry*; D003609:Dactinomycin / Q000737:chemistry; D003630:Daunorubicin / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D009942:Organometallic Compounds; D003609:Dactinomycin; D012428:Ruthenium; D004247:DNA; D003630:Daunorubicin