[Study of DNA identification in burned bones].
OBJECTIVE
For the purpose of solving a problems of DNA testing of burned bones.


METHODS
We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purification using the DNA IQ System.


RESULTS
The methods were found to be effective in removing the PCR inhibitors from the burned bone. Then the extracted DNA was successfully genotyped by using the florescence labeling STR multiplex method.


CONCLUSIONS
The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, precluding the utilization of traditional forensic DNA identification techniques.
Ye
Jian
J
Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. yejian_21@sina.com.cn
Ji
An-quan
AQ
Zhao
Xing-chun
XC
D001842:Bone and Bones / Q000737:chemistry*; D002056:Burns / Q000378:metabolism; D002593:Cetrimonium Compounds; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences; D013997:Time Factors
D002593:Cetrimonium Compounds; D004247:DNA