Structural information on nanomolecular systems revealed by FRET.
Our newly developed fluorescence resonance energy transfer (FRET) based technique, fluorescence nanotomography (FN), is used to determine the morphology and dynamics of some soft materials and bio-molecules by attaching donor (D) fluorophores and acceptors (A) to the investigated structure and using fluorescence lifetime measurements to reveal the D-A distance distribution function rhoDA(r). We report the effect of the limited sizes of the donor and acceptor, effect of porous polymer, and molecular structure and phase transition in phospholipid bilayers.
Rolinski
Olaf J
OJ
Department of Physics, University of Strathclyde, John Anderson Building, 107 Rottenrow, Glasgow G4 0NG, UK. o.j.rolinski@strath.ac.uk
Mathivanan
Chinnaraj
C
McNaught
Gillian
G
Birch
David J S
DJ
D000465:Algorithms; D003460:Crystallization / Q000379:methods*; D005240:Feasibility Studies; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008051:Lipid Bilayers / Q000032:analysis* / Q000737:chemistry*; D046911:Macromolecular Substances / Q000032:analysis / Q000737:chemistry; D008968:Molecular Conformation; D049329:Nanostructures / Q000032:analysis* / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D041622:Tomography, Optical / Q000379:methods*
D008051:Lipid Bilayers; D046911:Macromolecular Substances; D010743:Phospholipids