Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells.
In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 microM DATS significantly decreased the cell viability as compared with the control (P < 0.05) in dose and time dependent relations. Phenomena of cell number loss, shape deformation and lysis were observed after treatment with 100 microM DATS for 24 h. Cell cycle studies showed that J5 cells were significantly arrested in G2/M phase as the cells were treated with 100 microM DADS, 10, 50 or 100 microM DATS for 24 h (P < 0.05). DATS was more effective in arresting cells in G2/M phase than DADS, and the phenomena of arresting J5 cells in G2/M phase increased obviously in dose and time dependent relations. According to the Western blot analysis, DATS decreased cyclin-dependent kinase (Cdks)-Cdk7 (i.e. Cdc2 activate kinase) protein levels in J5 cells but increased cyclin B1 protein level. The modulation potency to cyclin B1 and Cdk7 expressions was in the order of DATS > DADS > DAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells, and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides.
Wu
C-C
CC
Department of Nutrition and Health Science, Fooyin University, Kaohsiung 831, Taiwan, ROC.
Chung
J G
JG
Tsai
S-J
SJ
Yang
J H
JH
Sheen
L Y
LY
D000498:Allyl Compounds / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis; D004220:Disulfides / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009822:Oils, Volatile / Q000494:pharmacology*; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D000972:Antineoplastic Agents, Phytogenic; D016213:Cyclins; D004220:Disulfides; D009363:Neoplasm Proteins; D009822:Oils, Volatile; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide; C038491:allyl sulfide; D018844:Cyclin-Dependent Kinases