title: A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells.
abstract: To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.
authors.affiliation: Plant Science Center, RIKEN (Institute of Physical and Chemical Research), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan. kenmat@psc.riken.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Taku
authors.initials: T
authors.lastname: Demura
authors.forename: Ivan
authors.initials: I
authors.lastname: Galis
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Horiguchi
authors.forename: Mami
authors.initials: M
authors.lastname: Sasaki
authors.forename: Gen
authors.initials: G
authors.lastname: Tashiro
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D002460:Cell Line; D032461:Chromosomes, Plant; D017931:DNA Primers; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary