title: Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE).
abstract: Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33 degrees C, this was reduced at 37 and 39 degrees C. Concomitantly, the cell growth was arrested at 37 and 39 degrees C compared with that at 33 degrees C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan. hosoyak@ms.toyama-mpu.ac.jp
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hosoya
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tomi
authors.forename: Megumi
authors.initials: M
authors.lastname: Takayama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Komokata
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakai
authors.forename: Taro
authors.initials: T
authors.lastname: Tokui
authors.forename: Kenji
authors.initials: K
authors.lastname: Nishimura
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ueda
authors.forename: Masuo
authors.initials: M
authors.lastname: Obinata
authors.forename: Satoko
authors.initials: S
authors.lastname: Hori
authors.forename: Sumio
authors.initials: S
authors.lastname: Ohtsuki
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Amidon
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Terasaki
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger