title: Long-term persistence of clonally expanded T cells in patients with polymyositis.
abstract: Polymyositis is a CD8(+) T-cell-mediated disease. T-cell clonal expansions are observed at disease onset, but little is known about their persistence over time. Qualitative and quantitative spectratyping demonstrated that PM relapse features dramatically perturbed blood T-cell repertoires but is not associated with the emergence of new T-cell clones. It is striking that patients in remission also maintained all their T-cell repertoire abnormalities. The clonally expanded T-cells displayed a memory phenotype, expressed intracellular perforin, and dramatically responded to IL-2, showing a potential to be reactivated upon appropriate conditions. These results indicate that persistent T-cell clonal expansion is an important feature of polymyositis.
authors.affiliation: Service de Médecine Interne, Hôpital Pitié-Salpêtrière, Paris, France. olivier.benveniste@psl.ap-hop-paris.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Benveniste
authors.forename: Serge
authors.initials: S
authors.lastname: Herson
authors.forename: Benoît
authors.initials: B
authors.lastname: Salomon
authors.forename: Dalia
authors.initials: D
authors.lastname: Dimitri
authors.forename: Hélène
authors.initials: H
authors.lastname: Trébeden-Nègre
authors.forename: Laetitia
authors.initials: L
authors.lastname: Jean
authors.forename: Véronique
authors.initials: V
authors.lastname: Bon-Durand
authors.forename: David
authors.initials: D
authors.lastname: Antonelli
authors.forename: David
authors.initials: D
authors.lastname: Klatzmann
authors.forename: Olivier
authors.initials: O
authors.lastname: Boyer
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D002999:Clone Cells; D006801:Humans; D008213:Lymphocyte Activation; D017285:Polymyositis / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology*; D013997:Time Factors