title: Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog.
abstract: To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Leigh Anne
authors.initials: LA
authors.lastname: Clark
authors.forename: Kate L
authors.initials: KL
authors.lastname: Tsai
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
authors.forename: Trina
authors.initials: T
authors.lastname: Guerra
authors.forename: Elaine A
authors.initials: EA
authors.lastname: Ostrander
authors.forename: Francis
authors.initials: F
authors.lastname: Galibert
authors.forename: Keith E
authors.initials: KE
authors.lastname: Murphy
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D005456:Fluorescent Dyes; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes