Ischemic heart disease down-regulates angiotensin type 1 receptor mRNA in human coronary arteries.
Angiotensin II is important in the development of cardiovascular disease. In the present study, angiotensin II receptor mRNA levels were quantified by real-time polymerase chain reaction (real-time PCR) in human coronary arteries from patients with ischemic heart disease and controls. Furthermore, the suitability of artery culture for studying angiotensin receptor changes was evaluated by in vitro pharmacology and real-time PCR. The angiotensin type 1 (AT1) receptor mRNA levels were down-regulated in human coronary arteries from patients with ischemic heart disease as compared to controls (P<0.05). Culture of coronary arteries for 48 h induced down-regulation of the angiotensin AT1 and AT2 receptor mRNA levels and also a less efficacious angiotensin II-induced vasoconstriction (Emax=103+/-2% before and 23+/-7% after artery culture, P<0.001). Artery culture may thus be a suitable method for studying angiotensin receptor regulation.
Wackenfors
Angelica
A
Department of Internal Medicine, Division of Experimental Vascular Research, Lund University Hospital, BMC A13, SE-221 84 Lund, Sweden.
Emilson
Malin
M
Ingemansson
Richard
R
Edvinsson
Lars
L
Malmsjö
Malin
M
D002478:Cells, Cultured; D003331:Coronary Vessels / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000502:physiology; D006801:Humans; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D017202:Myocardial Ischemia / Q000378:metabolism*; D009924:Organ Culture Techniques; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D044140:Receptor, Angiotensin, Type 1 / Q000096:biosynthesis* / Q000235:genetics; D044139:Receptor, Angiotensin, Type 2 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology
D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011188:Potassium