Genome-wide analysis of Kluyveromyces lactis in wild-type and rag2 mutant strains.
The use of heterologous DNA arrays from Saccharomyces cerevisiae has been tested and revealed as a suitable tool to compare the transcriptomes of S. cerevisiae and Kluyveromyces lactis, two yeasts with notable differences in their respirofermentative metabolism. The arrays have also been applied to study the changes in the K. lactis transcriptome owing to mutation in the RAG2 gene coding for the glycolytic enzyme phosphoglucose isomerase. Comparison of the rag2 mutant growing in 2% glucose versus 2% fructose has been used as a model to elucidate the importance of transcriptional regulation of metabolic routes, which may be used to reoxidize the NADPH produced in the pentose phosphate pathway. At this transcriptional level, routes related to the oxidative stress response become an interesting alternative for NADPH use.
Becerra
Manuel
M
Dpto. de Biología Celular y Molecular, Universidad de La Coruña, F. Ciencias, Campus de La Zapateira s/n 15075, La Coruña, Spain.
Tarrío
Nuria
N
González-Siso
M Isabel
MI
Cerdán
M Esperanza
ME
D005632:Fructose / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D016681:Genome, Fungal; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D009249:NADP / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics
D005632:Fructose; D009249:NADP; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose