Cobalt-protoporphyrin treatment enhances murine isoislets engraftment.
To study the effect of treatment with cobalt-protoporphyrin (CoPP) for the induction of the heme oxygenase-1 (HO-1) enzyme on islet engraftment donor mice received either a single intraperitoneal injection of CoPP (20 mg/kg body weight) 1 day prior to islet isolation or this injection plus a 9 day posttransplantation course of Copp. After a single injection of CoPP, the CoPP-induced islets contained higher HO-1 proteins than did the normal islets both at 12 (5.3 +/- 1.5 vs 0.1 +/- 0.1 ng/mg protein, P < .01) and at 30 hours (6.8 +/- 2.1 vs 0.4 +/- 0.3 ng/mg protein, P < .05), but not at 56 hours (1.9 +/- 0.8 vs 1.6 +/- 0.8 ng/mg protein, P > .05). In contrast, diabetic mice that received 75 CoPP-induced islets and a 9-day CoPP injection course posttransplantation showed better improvement in blood glucose levels and body weights than did the mice that only received CoPP-induced islets. Mice of both CoPP-treated groups displayed better improvement in glycemic control than mice that received control islets. At 8 weeks after transplantation, the insulin content of grafts from both CoPP groups was significantly higher than that in the control group. In conclusion, CoPP treatment for the induction of HO-1 enhances engraftment of islets in a syngeneic murine transplantation model.
Fu
S-H
SH
Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang-Gung Memorial Hospital, Taoyuan Hsien, Taiwan.
Hsu
B R-S
BR
Juang
J-H
JH
Chen
S-T
ST
Yang
T-Y
TY
Hsu
S
S
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011524:Protoporphyrins / Q000627:therapeutic use*
D001786:Blood Glucose; D011524:Protoporphyrins; C007095:cobaltiprotoporphyrin