[A study of genetic polymorphism of the STR locus D7S2846, D19S400 and D18S535 in Han population in Wenzhou].
OBJECTIVE
To study genetic polymorphism of D7S2846, D19S400 and D18S535 in Han population in Wenzhou.


METHODS
DNA was extracted with chelex-100 method from EDTA-blood samples of 194 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.


RESULTS
6 alleles and 15 genotypes of D7S2846, 10 alleles and 36 genotypes of D19S400, 8 alleles and 26 genotypes of D18S535 was observed. The heterozygosities of the three loci are 0.644, 0.724 and 0.772; The power of discriminating are 0.854, 0.940 and 0.938.


CONCLUSIONS
The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium (P>0.05), so the three loci can be used in forensic medicine and in other genetic researches.
Zhang
Hong-qin
HQ
Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027, China.
Wu
Shu-zhen
SZ
Bi
Yun-tian
YT
D000483:Alleles; D002681:China / Q000208:ethnology; D004247:DNA / Q000302:isolation & purification; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D004247:DNA