title: Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing.
abstract: An siRNA nanocarrier formed through self-assembly of PEG-based block catiomer possessing two distinct amino groups with different pKa values in a side chain was developed. This design provided the carrier with a sufficient siRNA complexation and an assumed buffering capacity in the endosomes, allowing it to exhibit remarkable gene knockdown abilities as well as sufficient serum tolerability.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Itaka
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishiyama
authors.forename: Woo-Dong
authors.initials: WD
authors.lastname: Jang
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Kozo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kataoka
mesh_terms: D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003959:Diamines / Q000737:chemistry*; D020868:Gene Silencing; D006801:Humans; D007700:Kinetics; D049329:Nanostructures / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics
chemical_list: D002412:Cations; D003959:Diamines; D010455:Peptides; D034741:RNA, Small Interfering; C033141:poly-beta-benzyl-aspartate; D011092:Polyethylene Glycols; D004247:DNA