title: [Polarograhic adsorptive wave of protein hydrolysate in Pb2+ and sodium hydroxide solution and its application].
abstract: OBJECTIVE
  To propose a new simple and sensitive voltammetric method for determination of proteins.
  
  
  METHODS
  Protein with sulfhydryl or disulfide bond in 0.5 mol x L(-1) NaOH, 1.5 x 10(-4) mol x L(-1) Pb2+ and 0.02% tetrabutylammonium iodide was heated in boiling water for 5 minutes. The reactive product gave a well defined reductive adsorption wave at -0.66 V (vs SCE) by means of single sweep polarography, and the height of derivative wave was proportional to the concentration of proteins.
  
  
  RESULTS
  The peak height was linearly proportional to bovine serum albumin (BSA) or human serum albumin (HSA) concentration in range of 7.5 x 10(-10) -3.0 x 10(-7) mol x L(-1) (r(BSA) = 0.9995, and r(HSA) = 0.9990). The detection limit of BSA or HSA was 3.0 x 10(-10) mol x L(-1). For lysozyme (Lyso), the concentration range was from 1.4 x 10(-8) to 1.3 x 10(-6) mol x L(-10 (r(Lyso) = 0.9997) and the detection limit was 7.0 x 10(-9) mol x L(-1).
  
  
  CONCLUSIONS
  The method is simple, rapid, sensitive and applicable to the assay of diluted human serum albumin samples.
authors.affiliation: College of Chemistry and Life Science, South Central University for Nationalities, Wuhan 430074, China.
authors.forename: Jin-Gui
authors.initials: JG
authors.lastname: Lan
authors.forename: Deng-Bai
authors.initials: DB
authors.lastname: Luo
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D006801:Humans; D007854:Lead; D009113:Muramidase / Q000032:analysis; D011048:Polarography / Q000379:methods*; D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D012972:Sodium Hydroxide
chemical_list: D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D007854:Lead; D012972:Sodium Hydroxide; C009405:tetrabutylammonium; D009113:Muramidase