title: Evaluation of two rapid assays for detecting Cryptosporidium parvum in calf feces.
abstract: OBJECTIVE
  To evaluate 2 rapid, patient-side assays for detection of Cryptosporidium parvum in feces from neonatal calves with diarrhea.
  
  
  METHODS
  Diagnostic test evaluation Sample Population-Fecal samples from 96 neonatal (1 to 30 days old) calves with diarrhea.
  
  
  METHODS
  Results of the rapid assays were compared with results of microscopic examination of fecal smears that had been stained with diamant fuchsin stain.
  
  
  RESULTS
  One of the rapid assays correctly identified 56 of 62 (90%) fecal samples positive for C. parvum oocysts and 33 of 34 (97%) fecal samples negative for oocysts. The other assay correctly identified 53 of 62 (85%) fecal samples positive for oocysts and 33 of 34 (97%) fecal samples negative for oocysts.
  
  
  CONCLUSIONS
  Results suggest that these 2 rapid assays are accurate when used to detect C. parvum in fecal samples from neonatal calves with diarrhea.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Janna L
authors.initials: JL
authors.lastname: Muccio
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Grooms
authors.forename: Linda S
authors.initials: LS
authors.lastname: Mansfield
authors.forename: Annabel G
authors.initials: AG
authors.lastname: Wise
authors.forename: Roger K
authors.initials: RK
authors.lastname: Maes
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005260:Female; D008297:Male; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013997:Time Factors