title: Expression pattern of FCRL (FREB, FcRX) in normal and neoplastic human B cells.
abstract: FCRL (also known as FREB and FcRX) is a recently described member of the family of Fc receptors for immunoglobulin G (IgG). In the present study we analysed its expression in normal and neoplastic lymphoid tissue using immunohistochemical techniques. FCRL was preferentially expressed in a proportion of germinal centre cells and, more weakly, in mantle zone B cells. In addition, strong labelling was observed in marginal zone B cells in the spleen, representing one of the few markers for this cell type. The majority of cases of small B-cell lymphoma, diffuse large B-cell lymphoma and lymphocyte predominance Hodgkin's disease were positive for FCRL. However, the number of positive cells varied widely, and in consequence we could not define a cut-off that distinguished subsets of diffuse large B-cell lymphoma. Our results also showed that FCRL tended to be negative in T-cell-rich B-cell lymphoma and in classical Hodgkin's disease. FCRL may therefore represent a novel marker for normal B cells (e.g. splenic marginal zone cells) and may also be useful as a potential marker of B-cell neoplasms.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, UK.
authors.forename: Noraidah
authors.initials: N
authors.lastname: Masir
authors.forename: Margaret
authors.initials: M
authors.lastname: Jones
authors.forename: Michela
authors.initials: M
authors.lastname: Pozzobon
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.forename: Olga Y
authors.initials: OY
authors.lastname: Volkova
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Mechetina
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Taranin
authors.forename: David Y
authors.initials: DY
authors.lastname: Mason
mesh_terms: D001402:B-Lymphocytes / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D006689:Hodgkin Disease / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016393:Lymphoma, B-Cell / Q000737:chemistry*; D011961:Receptors, Fc / Q000032:analysis*
chemical_list: D015415:Biomarkers; C452186:FCRLA protein, human; D011961:Receptors, Fc