Differential expression of bone morphogenetic protein 4-6 (BMP-4, -5, and -6) and growth differentiation factor-9 (GDF-9) during ovarian development in neonatal pigs.
Growth differentiation factor-9 (GDF-9) and bone morphogenetic proteins (BMPs), comprise the largest subgroups of ligands in the TGF-beta superfamily, and have been shown to be involved in follicle development in mammals. However, whether these factors are involved in folliculogenesis in pigs is still unknown. The present study was performed to determine the relationships between early folliculogenesis and the expression of GDF-9 and BMP (BMP-4, -5 and -6) mRNAs in neonatal pigs. Ovaries were removed at 5, 16, 28 and 39 days after birth to examine the follicular population (the right ovary of each animal) and to detect mRNA expression (the left ovary of each animal). Primordial follicles accounted for >80% of the ovarian follicles from 5 days until 39 days after birth. A marked increase in primary follicles and the appearance of secondary follicles were observed in the ovaries at 28 days after birth. BMP-4, -5, and -6 and GDF-9 mRNAs were expressed by ovaries at 5-, 16-, 28- and 39-day-old pigs. The peak expression of BMP-4, -5, and -6 and GDF-9 mRNAs was observed in the ovaries at 5, 39, 28 and 16 days, respectively, after birth. These data demonstrate that folliculogenesis in piglets might be controlled by the interaction with these factors. We conclude that BMPs and GDF-9 may have distinct functions in several stages of follicle development in neonatal pig ovaries.
Shimizu
Takashi
T
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan. shimizut@idac.tohoku.ac.jp
Yokoo
Masaki
M
Miyake
Yuko
Y
Sasada
Hiroshi
H
Sato
Eimei
E
D000818:Animals; D000831:Animals, Newborn; D055415:Bone Morphogenetic Protein 4; D055417:Bone Morphogenetic Protein 5; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055429:Growth Differentiation Factor 9; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D006080:Ovarian Follicle / Q000254:growth & development*; D010053:Ovary / Q000254:growth & development; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine / Q000254:growth & development*
D055415:Bone Morphogenetic Protein 4; D055417:Bone Morphogenetic Protein 5; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins; D055429:Growth Differentiation Factor 9; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger