Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog.
To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.
Clark
Leigh Anne
LA
Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Tsai
Kate L
KL
Steiner
Jörg M
JM
Williams
David A
DA
Guerra
Trina
T
Ostrander
Elaine A
EA
Galibert
Francis
F
Murphy
Keith E
KE
D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D005456:Fluorescent Dyes; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*
D005456:Fluorescent Dyes