Plant cuticular lipid export requires an ABC transporter.
A waxy protective cuticle coats all primary aerial plant tissues. Its synthesis requires extensive export of lipids from epidermal cells to the plant surface. Arabidopsis cer5 mutants had reduced stem cuticular wax loads and accumulated sheetlike inclusions in the cytoplasm of wax-secreting cells. These inclusions represented abnormal deposits of cuticular wax and resembled inclusions found in a human disorder caused by a defective peroxisomal adenosine triphosphate binding cassette (ABC) transporter. We found that the CER5 gene encodes an ABC transporter localized in the plasma membrane of epidermal cells and conclude that it is required for wax export to the cuticle.
Pighin
Jamie A
JA
Department of Botany, University of British Columbia (UBC), 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.
Zheng
Huanquan
H
Balakshin
Laura J
LJ
Goodman
Ian P
IP
Western
Tamara L
TL
Jetter
Reinhard
R
Kunst
Ljerka
L
Samuels
A Lacey
AL
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D019281:Dimerization; D017343:Genes, Plant; D002479:Inclusion Bodies / Q000648:ultrastructure; D050356:Lipid Metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014617:Vacuoles / Q000648:ultrastructure; D014885:Waxes / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; C493174:CER5 protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D014885:Waxes