title: GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori.
abstract: Bombyx mori (ShunreixShogetsu) is sensitive to Cry1Aa and resistant to Cry1Ac, both insecticidal proteins of Bacillus thuringiensis. Cry1Aa passed through the peritrophic membrane (PM) much faster (0.37 microg/mm2 PM/h) than Cry1Ac (0.05 microg/mm2 PM/h) during the initial observation period. Both Cry1Aa and Cry1Ac bound to the PM but only the binding of Cry1Ac was specifically inhibited by N-acetylgalactosamine (GalNAc). When Cry1Ac was pretreated with GalNAc, Cry1Ac permeated the PM much faster. These results suggested that Cry1Ac bound a PM protein via GalNAc on a sugar side chain. The role of the PM on Cry1Ac resistance of B. mori was briefly discussed.
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Shitomi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hori
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology*; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000493:pharmacokinetics*; D001427:Bacterial Toxins / Q000493:pharmacokinetics*; D012831:Bombyx / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004731:Endotoxins / Q000493:pharmacokinetics*; D006460:Hemolysin Proteins
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D000116:Acetylgalactosamine