Use of an improved zirconyl hematoxylin stain in the diagnosis of Barrett's esophagus.
Barrett's esophagus is a precancerous condition characterized by replacement of the normal stratified squamous epithelium by a simple columnar epithelium with goblet cells that secrete an acidic mucin. As originally formulated, fresh solutions of zirconyl hematoxylin stain goblet cells poorly. An improved formula, quintupling the amount of oxidant, yields zirconyl hematoxylin solutions that stain goblet cells darkly even when fresh. The improved zirconyl hematoxylin can be used in place of alcian blue in the diagnosis of Barrett's esophagus. The ingredients of zirconyl hematoxylin are always readily available and are generally recognized as safe.
McNulty
Jeannette M
JM
School of Graduate Medical Sciences, Barry University, Miami Shores, FL, 33161, USA.
Kambour
Michael J
MJ
Smith
Allen A
AA
D000423:Alcian Blue; D001471:Barrett Esophagus / Q000175:diagnosis* / Q000473:pathology; D004396:Coloring Agents; D004848:Epithelium / Q000473:pathology; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D020397:Goblet Cells / Q000473:pathology; D006416:Hematoxylin / Q000031:analogs & derivatives*; D006801:Humans; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000473:pathology
D004396:Coloring Agents; C418048:zirconyl hematoxylin; D000423:Alcian Blue; D006416:Hematoxylin