Role of polyamines in the function of nuclear transcription factor NF-kappaB in breast cancer cells.
The aim of the work was the investigation of the influence of polyamines on formation of nuclear transcription factor kappa B (NF-kappaB) complex with specific DNA-sequences nuclear response elements (NRE), and the study of content of p50 and p65 (NF-kappaB subunits) in breast tumor cells under polyamines metabolism modulation.


METHODS
The model experiments in vitro were carried out on cultured MCF-7 cells, in vivo experiments -- on experimental Ca755 and Walker-256 tumors using surface plasmon resonance (SPR) and Western-blotting analysis.


RESULTS
The data have demonstrated that polyamines falicitated p50 -- NRE binding. Under conditions of polyamine depletion, an increase of content of both p50 and p65 subunits of NF-kappaB in the nucleus has been registered (especially that of p50 subunits). Upon addition of alpha-DFMO + putrescine to the culture medium of MCF-7 cells, the decrease of the levels of p50 and p65 proteins in the nuclei has been observed.


CONCLUSIONS
Polyamines influence the activity of NF-kappaB transcription factor in breast tumor cells.
Zaletok
Sophia
S
R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine. sophiazaletok@hotmail.com.
Alexandrova
Nataliya
N
Berdynskykh
Nina
N
Ignatenko
Nataliya
N
Gogol
Sergiy
S
Orlovsky
Oleksiy
O
Tregubova
Nadiya
N
Gerner
Eugene
E
Chekhun
Vasyl
V
D000818:Animals; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002279:Carcinoma 256, Walker / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D000518:Eflornithine / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D020349:Surface Plasmon Resonance; D051996:Transcription Factor RelA; D015533:Transcriptional Activation / Q000187:drug effects
D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D009363:Neoplasm Proteins; D011073:Polyamines; D051996:Transcription Factor RelA; D013096:Spermine; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine