title: Modulation of murine macrophage function by methamphetamine.
abstract: The abuse of methamphetamine (MA) is an increasingly growing problem globally and produces serious side effects. In the present study, the immunomodulating effects of MA were examined on murine peritoneal macrophages after MA (5 mg/kg body weight) was administered daily orally for 2 wk. When purified macrophages were stimulated with lipopolysaccharide (LPS), the tumoricidal activity induced by LPS was significantly suppressed by MA. MA also inhibited poly I:C-induced antiviral activity in macrophages and decreased the number of peritoneal macrophages. FACS analysis showed that the expression of CD14 was markedly decreased by MA in LPS-stimulated macrophages. The production of nitric oxide (NO) and tumor necrosis factor (TNF)-alpha: which are known to be major effector molecules in macrophage-mediated cytotoxicity, was decreased by MA. MA produced a significant effect on phagocytosis and interleukin-1 (IL-1) and IL-6 at 14 d. In addition, the level of hydrogen peroxide (H2O2) was not altered by MA. Taken together, these data indicate that MA has a differential immunomodulating effect on macrophage secretory and cellular activities.
authors.affiliation: College of Pharmacy, Sungkyunkwan University, Suwon, Kyunggi-do, South Korea.
authors.forename: Sang-Whan
authors.initials: SW
authors.lastname: In
authors.forename: Eun-Wha
authors.initials: EW
authors.lastname: Son
authors.forename: Dong-Kwon
authors.initials: DK
authors.lastname: Rhee
authors.forename: Suhkneung
authors.initials: S
authors.lastname: Pyo
mesh_terms: D000818:Animals; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000032:analysis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D008694:Methamphetamine / Q000633:toxicity*; D051379:Mice; D016877:Oxidants / Q000032:analysis; D010537:Peritoneum
chemical_list: D000697:Central Nervous System Stimulants; D018950:Lipopolysaccharide Receptors; D016877:Oxidants; D008694:Methamphetamine; D006861:Hydrogen Peroxide