title: A method for fast determination of psoralens in oral solutions of phytomedicines using liquid chromatography.
abstract: A method for sample preparation and analysis by high performance liquid chromatography with UV detection (HPLC-UV) has been developed for routine analysis of psoralen and bergapten, photosensitizing compounds, in oral solutions of phytomedicines employed in Brazil for some illnesses. The linearity, accuracy, the inter- and intra-day precision of the procedure were evaluated. Calibration curves for psoralen and bergapten were linear in the range of 1.0-600.0 microg ml(-1) and 1.0-400.0 microg ml(-1) respectively. The recoveries of the psoralens in the oral solutions analysed were 94.43-99.97%. The percentage coefficient of variation (CV) of the quantitative analysis of the psoralens in the products analysis was within 5%. In inter-equipment study was employed gas chromatography-flame ionization (CG-FID) detection.
authors.affiliation: Departamento de Química, Universidade Federal de Mato Grosso do Sul, Caixa Postal 649, 79070-900 Campo Grande/MS, Brazil. adrielias@yahoo.com.br
authors.forename: Adriana Elias
authors.initials: AE
authors.lastname: Pires
authors.forename: Neli Kiko
authors.initials: NK
authors.lastname: Honda
authors.forename: Cláudia Andréa Lima
authors.initials: CA
authors.lastname: Cardoso
mesh_terms: D000078223:5-Methoxypsoralen; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005410:Flame Ionization; D011564:Furocoumarins / Q000032:analysis*; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000032:analysis; D019999:Pharmaceutical Solutions; D017319:Photosensitizing Agents / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011564:Furocoumarins; D019999:Pharmaceutical Solutions; D017319:Photosensitizing Agents; D000078223:5-Methoxypsoralen; D008730:Methoxsalen