title: [Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells].
abstract: OBJECTIVE
  To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G(1)/S-phase renal tubule epithelial cells (RTECs).
  
  
  METHODS
  G1-phase RTECs were divided into cantharidin-treated group, exposed to sodium cyanide (CN) and cantharidin, hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography.
  
  
  RESULTS
  The concentration of CN exposure for 1 h, ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50+/-0.26 mmol/g protein, 4.25+/-0.11 mmol/g protein, 8.58+/-0.13 mmol/g protein, respectively, P<0.01).
  
  
  CONCLUSIONS
  Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.
authors.affiliation: Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mailsq@fmmu.edu.cn
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.forename: Yu-jia
authors.initials: YJ
authors.lastname: Yao
authors.forename: Ru-ying
authors.initials: RY
authors.lastname: Li
authors.forename: Cui-ling
authors.initials: CL
authors.lastname: Ding
authors.forename: Li-chao
authors.initials: LC
authors.lastname: Hou
authors.forename: Xu-de
authors.initials: XD
authors.lastname: Sun
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002193:Cantharidin / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D016193:G1 Phase; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D016196:S Phase; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; D000255:Adenosine Triphosphate; D002193:Cantharidin