[Concentration of soluble CD4, CD8, CD25 receptors as well IFN-gamma and IL-4 released by lymphocyte of chronic Lyme patients cultured with 3 genotypes of Borrelia burgdorferi].
The aim of the study was the evaluation of sCD4, sCD8, sCD25, IFN-gamma and IL-4 released in the supernatant of lymphocyte cultures from patients with chronic Lyme stimulated by B. burgdorferi. Three genotypes of B. burgdorferi: sensu stricto B-31, B. garinii 20047 and B. afzelii--VS 46110 were used for stimulation. The study group consisted of 23 patients (13 males and 10 females). The control group (K) consisted of 10 healthy people. Peripheral blood mononuclear cells were incubated in atmosphere of 5% CO2 at 37 degrees C after the addition of spirochetes of B. afzelii (VS 46110), B. garinii (20047), B. burgdorferi sensu stricto (B-31) in the concentration of 10(8) spirochetes/well, for 5 days. The supernatants were analyzed for concentration of sCD4, sCD8, sCD25 and IL-4, IFN-gamma by means of ELISA method. The increase in sCD4, sCD8 and sCD25 concentrations indicates the activation of these lymphocytes with CD4, CD8, CD25 receptors in this group of patients. A significantly higher concentration of IFN-gamma shows the long-lasting cellular response with the advantage of Th1 type profile cytokines. All used strains had immunogenic properties but B. afzelii had the strongest in patients with chronic borreliosis.
Zajkowska
Joanna M
JM
Klinika Chorób Zakaźnych i Neuroinfekcji Akademii Medycznej w Biatymstoku. zajkowsk@kki.pl
Swierzbińska
Renata
R
Pancewicz
Sławomir A
SA
Kondrusik
Maciej
M
Hermanowska-Szpakowicz
Teresa
T
D000328:Adult; D025065:Borrelia burgdorferi / Q000276:immunology*; D015704:CD4 Antigens / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D008193:Lyme Disease / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011044:Poland; D011948:Receptors, Antigen, T-Cell / Q000097:blood*; D015375:Receptors, Interleukin-2 / Q000097:blood*
D015704:CD4 Antigens; C067361:CD8 receptor; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma