title: KIT gene deletions at the intron 10-exon 11 boundary in GI stromal tumors.
abstract: Most gastrointestinal stromal tumors (GISTs) harbor oncogenic mutations in the KIT gene, and the majority of these mutations affect the juxtamembrane domain of the kinase encoded by exon 11. Screening GISTs for KIT gene mutations is important for translational research studies and for providing prognostic information on the likelihood of tumor response to treatment with the kinase inhibitor imatinib mesylate (Gleevec). In a series of GISTs analyzed in our laboratory by a combination of denaturing HPLC and direct DNA sequencing, we identified 19 cases with KIT exon 11 deletions that included from 1 to 14 bp of intron 10 sequence and resulted in loss of the normal splice acceptor site at the beginning of exon 11. Predicted use of the next potential splice-acceptor site was confirmed by cDNA sequencing in 4 cases. Thus, the resulting mutant isoform, deletion KPMYEVQWK 550-558, was the same in all 19 cases. Only two other examples of deletions across the intron 10-exon 11 boundary have been reported, yet among 722 GISTs analyzed in our laboratories these deletions were not uncommon, accounting for 3.9% of exon 11 mutations and 2.6% of all tumors. Loss of KIT intron 10 sequences may be under-recognized if the forward primer is too close to exon 11, or if cases are examined exclusively at the cDNA level. Laboratories that offer clinical screening for KIT mutations in GI stromal tumors should be aware of this class of mutations.
authors.affiliation: Department of Pathology, Oregon Health & Science University Cancer Institute, Portland, OR 97239, USA. corlessc@ohsu.edu.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Corless
authors.forename: Laura
authors.initials: L
authors.lastname: McGreevey
authors.forename: Ajia
authors.initials: A
authors.lastname: Town
authors.forename: Arin
authors.initials: A
authors.lastname: Schroeder
authors.forename: Troy
authors.initials: T
authors.lastname: Bainbridge
authors.forename: Patina
authors.initials: P
authors.lastname: Harrell
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Fletcher
authors.forename: Michael C
authors.initials: MC
authors.lastname: Heinrich
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001549:Benzamides; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D005770:Gastrointestinal Neoplasms / Q000235:genetics*; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics*; D017353:Gene Deletion; D006720:Homozygote; D006801:Humans; D000068877:Imatinib Mesylate; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010879:Piperazines / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D020033:Protein Isoforms; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D011743:Pyrimidines / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome
chemical_list: D001549:Benzamides; D018076:DNA, Complementary; D010879:Piperazines; D020033:Protein Isoforms; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D004247:DNA; D019009:Proto-Oncogene Proteins c-kit