Generation and characterisation of monoclonal antibodies to oleanolic acid.
Monoclonal antibodies to oleanolic acid, a pentacyclic triterpene, were generated using the hybridoma fusion method described by Kohler and Milstein . Protein conjugates of the target molecule for immunisation were prepared either by directly linking the isoprenoid to different protein carriers via the carboxylic function or after introduction of a succinic spacer between the protein carrier and position 3 of the target molecule. Antibodies of three different cell lines were further analysed by competitive ELISA and were shown to be directed either against both rings A and B or to ring E of the pentacyclic system. Thus these antibodies can be used in the specific detection of oleanolic acid itself or of a broad range of oleanolic acid derivatives sharing a specific structural moiety. Therefore these antibodies may be interesting tools for the screening of putative terpenoid-containing plants.
Brand
K M
KM
Institute of Pharmaceutical Biology, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
Klein
C
C
Zündorf
I
I
Dingermann
T
T
Knöss
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009828:Oleanolic Acid / Q000737:chemistry / Q000276:immunology*; D008517:Phytotherapy; D010946:Plants, Medicinal
D000911:Antibodies, Monoclonal; D009828:Oleanolic Acid