title: Characterization of a highly inducible novel CC chemokine from differentiated rainbow trout (Oncorhynchus mykiss) macrophages.
abstract: A full-length cDNA clone encoding a novel trout CC chemokine was identified in expressed sequence tags generated from lipopolysaccharide (LPS)-stimulated in vitro differentiated macrophages isolated from the head kidney of the rainbow trout (Oncorhynchus mykiss). The putative 101-amino-acid protein is 38% similar to Macaca mulatta CCL4 (macrophage inflammatory protein 1beta) but is also similar to several other related mammalian CC chemokines, including human Act-2. Real-time PCR and conventional RT-PCR revealed significant up-regulation of transcript levels of the trout CCL4-like mRNA in LPS-stimulated in vitro differentiated macrophages. In unstimulated trout, CCL4-like mRNA expression was detected at different levels in all tissues tested, whereas in LPS-challenged animals (6 mg/kg), CCL4-like mRNA increased in intestine, ovary and spleen at both 24 h and 72 h post-injection. In gills, CCL4-like mRNA expression was inhibited after LPS administration. Based on the highly regulated expression pattern exhibited by the trout CCL4-like mRNA, it is likely that this chemokine plays an important regulatory role in the immune response of trout.
authors.affiliation: Unitat de Fisiologia Animal, Departament de Biologia Cellular, Fisiologia i d'Immunologia, Facultat de Ciencies, Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain. simon.mackenzie@uab.es
authors.forename: S
authors.initials: S
authors.lastname: Mackenzie
authors.forename: C
authors.initials: C
authors.lastname: Liarte
authors.forename: D
authors.initials: D
authors.lastname: Iliev
authors.forename: J V
authors.initials: JV
authors.lastname: Planas
authors.forename: L
authors.initials: L
authors.lastname: Tort
authors.forename: F W
authors.initials: FW
authors.lastname: Goetz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019742:Chemokines, CC / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000276:immunology*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D019742:Chemokines, CC; D012333:RNA, Messenger