OK205 regulates production of inflammatory cytokines in HMC-1 cells.
OK205 is a traditional Korean prescription containing water-soluble chitosan, glucosamine HCl, chondroitin sulfate, and extract of herbal medicine, and has been used commercially to treat rheumatoid arthritis (RA). Because infiltrated mast cells and their mediators may contribute to the initiation and progression of the inflammatory process and matrix degradation of RA, we tested the inhibitory effects of OK205 on cytokine production in a human mast cell line (HMC-1 cells). Production of tumor necrosis factor-alpha was significantly decreased to 0.091+/-0.010 ng/ml after treatment of HMC-1 cells with OK205 100 microg/ml. The inhibition rate was about 43.57%. In addition, production of interleukin-6 in OK205 1 pg/ml-treated cells was 2.779+/-0.071 ng/ml, and the inhibition rate was about 50.22%. However, OK205 did not significantly inhibit the production of interleukin-8. These findings may help in understanding the mechanism of action of OK205, leading to control of mast cells in inflammatory conditions like RA.
Choi
In-Young
IY
College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Jung
Hyuk-Sang
HS
Kim
Hyung-Ryong
HR
Lee
Eon-Jeong
EJ
Lee
Eun-Hee
EH
Shin
Tae-Yong
TY
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan; D002809:Chondroitin Sulfates; D016207:Cytokines / Q000096:biosynthesis*; D005944:Glucosamine; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008518:Medicine, East Asian Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D000093242:Interleukin-6 Inhibitors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D048271:Chitosan; D002809:Chondroitin Sulfates; D016207:Cytokines; D005944:Glucosamine; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C499385:OK205 Korean medicine