Hematopoietic development of human embryonic stem cells in culture.
The isolation of embryonic stem (ES) cells from human preimplantation blastocytes creates an exciting new starting point to analyze the earliest stages of human blood development. This chapter describes two methods for the promotion of hematopoietic differentiation of human ES cells: stromal cell co-culture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic benefits.
Tian
Xinghui
X
Stem Cell Institute and Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Kaufman
Dan S
DS
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D051379:Mice; D017154:Stromal Cells / Q000502:physiology; D039901:Totipotent Stem Cells / Q000502:physiology*