Separation of racemic 2,4-dinitrophenyl amino acids on 9-O-(phenyloxycarbonyl)quinine-bonded carbon-clad zirconia in reversed-phase liquid chromatography.
Zirconia is known to be one of the best materials for the chromatographic support due to its excellent chemical, thermal, and mechanical stability. In this work, we report preparation and use of 9-O-(phenyloxycarbonyl)quinine-bonded carbon-clad zirconia (QNCZ) as a chiral stationary phase (CSP) for separation of N-(2,4-dinitrophenyl) (DNP)-amino acids (AAs) enantiomers in reversed-phase liquid chromatography. Retention and enantioselectivity of the QNCZ CSP were compared with those of quinine 3-triethoxysilylpropylcarbamate-coated zirconia (QNZ) and quinine 3-triethoxysilylpropylcarbamate-bonded silica (QNS). The QNCZ CSP showed in general the better enantioselectivity for most of the amino acids studied.
Park
Jung Hag
JH
Department of Chemistry, College of Science, Yeungnam University, 214-1 Daedong, Kyongsan 712-749, Republic of Korea. jhpark@yu.ac.kr
Lee
Joon Woo
JW
Kwon
Sang Hyun
SH
Cha
Jin Soon
JS
Carr
Peter W
PW
McNeff
Clayton V
CV
D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004136:Dinitrobenzenes / Q000302:isolation & purification*; D007202:Indicators and Reagents; D011803:Quinine / Q000031:analogs & derivatives* / Q000737:chemistry*; D012822:Silicon Dioxide; D013237:Stereoisomerism; D015040:Zirconium / Q000737:chemistry*
C496815:9-O-(phenyloxycarbonyl)quinine; D000596:Amino Acids; D004136:Dinitrobenzenes; D007202:Indicators and Reagents; D012822:Silicon Dioxide; D011803:Quinine; D015040:Zirconium; C028541:zirconium oxide