Overexpression of the Wilms' tumor gene W T1 in primary astrocytic tumors.
Expression of the Wilms' tumor gene W T1 in primary astrocytic tumors was examined using a quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or immunohistochemistry. Real-time RT-PCR showed that W T1 mRNA was expressed at various levels in all of the 25 astrocytic tumors examined. Immunohistochemical analysis showed that W T1 protein was expressed in 5 of 6 low-grade astrocytic tumors (grade I-II) and all of 18 high-grade ones (grade III-IV), and that expression levels of W T1 protein in high-grade tumors were significantly higher than those in low-grade ones. W T1 protein was not detected in the normal glial cells contained in the tumor specimens. Furthermore, treatment with W T1 antisense oligomers specifically inhibited growth of glioblastoma cell lines, U87-MG, A172, and T-98G. These results may indicate that the W T1 gene plays an important role in tumorigenesis of primary astrocytic tumors.
Oji
Yusuke
Y
Department of Bioinformatics, Osaka University Graduate School of Medicine, 1-7 Yamada-Oka, Suita, Osaka 565-0871, Japan.
Suzuki
Tsuyoshi
T
Nakano
Yoko
Y
Maruno
Motohiko
M
Nakatsuka
Shin-Ichi
S
Jomgeow
Tanyarat
T
Abeno
Sakie
S
Tatsumi
Naoya
N
Yokota
Asumi
A
Aoyagi
Sayaka
S
Nakazawa
Tsutomu
T
Ito
Ken
K
Kanato
Keisuke
K
Shirakata
Toshiaki
T
Nishida
Sumiyuki
S
Hosen
Naoki
N
Kawakami
Manabu
M
Tsuboi
Akihiro
A
Oka
Yoshihiro
Y
Aozasa
Katsuyuki
K
Yoshimine
Toshiki
T
Sugiyama
Haruo
H
D000293:Adolescent; D000368:Aged; D001254:Astrocytoma / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000378:metabolism*
D012333:RNA, Messenger; D025721:WT1 Proteins