[Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells].
The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
Zhang
Wei
W
State Key Laboratory of Experimental Hematology, Institute of Hematology, Chenese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
Ge
Wei
W
Li
Chang-Hong
CH
You
Sheng-Guo
SG
Han
Qin
Q
Deng
Wei-Min
WM
Zhao
Chun-Hua
CH
D001854:Bone Marrow Cells / Q000502:physiology*; D018106:CD28 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D006801:Humans; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D015854:Up-Regulation
D018106:CD28 Antigens; D016827:CD8 Antigens