[The influence of medicine to the expression of cell adhesion molecule in experimental otitis media with effusion].
OBJECTIVE
To study the influence of Cefaclor and Prednisonum to the expression of cell adhesion molecule in experimental otitis media with effusion (OME).


METHODS
SD rats models of OME were made using formalin killed nontypeable Hemophilus influenzae and the expression of adhesion molecules Mac-1 and L-Selectin was analysed by flow cytometry using white blood cell obtained by cardiac puncture in control, bacterial and medicine groups. The pathology of middle ear mucosa was investigated to understand the relationship between the expression of adhesion molecules and the pathology.


RESULTS
It was found that the expression of adhesion molecules Mac-1 and L-Selection in bacteria group increased obviously with pathological change of the middle ear mucosa. Cefaclor and Prednisonum could decrease the expression of adhesion molecules and inhibited the leukocyte accumulation in the middle ear cavity though there was no significant differences except for the L-Selectin in the Cefaclor group between the bacteria group and the medicine group.


CONCLUSIONS
Dead bacteria in the middle ear may act as an antigen to induce the inflammatory response and effusion of the middle ear. The expression of adhesion molecules Mac-1 and L-Selectin is highly related to the inflammatory response. Cefaclor and Prednisonum may improve otitis media with effusion, which may related to the decreasing expression of Mac-1 and L-Selectin.
Wang
Shengzi
S
Department of Radiotherapy, EENT Hospital, Shanghai Fudan University, Shanghai 200031.
Yan
Yuehua
Y
Wang
Shuyi
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002433:Cefaclor / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D005938:Glucocorticoids / Q000494:pharmacology; D019041:L-Selectin / Q000096:biosynthesis; D008297:Male; D010034:Otitis Media with Effusion / Q000188:drug therapy* / Q000378:metabolism; D011241:Prednisone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000900:Anti-Bacterial Agents; D015815:Cell Adhesion Molecules; D005938:Glucocorticoids; D019041:L-Selectin; D002433:Cefaclor; D011241:Prednisone