title: Genotype analysis of Malassezia restricta as the major cutaneous flora in patients with atopic dermatitis and healthy subjects.
abstract: Lipophilic yeasts of the genus Malassezia colonize the skin surface of humans and are an exacerbating factor in atopic dermatitis (AD). Two species, M. restricta and M. globosa are major cutaneous microflora in both AD patients and healthy subjects. We compared the DNA sequences of the intergenic spacer (IGS) region, located between the 26S and 5S rRNA genes of M. restricta colonizing the skin surfaces of 13 AD patients and 12 healthy subjects, and of three CBS stock strains as references. The IGS 1 sequences were divided into two major groups, corresponding to AD patients and healthy subjects. These findings suggest that a specific genotype of M. restricta plays a significant role in AD, although M. restricta commonly colonizes both AD patients and healthy subjects.
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. sugita@my-pharm.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.forename: Mami
authors.initials: M
authors.lastname: Tajima
authors.forename: Misato
authors.initials: M
authors.lastname: Amaya
authors.forename: Ryoji
authors.initials: R
authors.lastname: Tsuboi
authors.forename: Akemi
authors.initials: A
authors.lastname: Nishikawa
mesh_terms: D000328:Adult; D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D003876:Dermatitis, Atopic / Q000382:microbiology*; D003881:Dermatomycoses / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D012867:Skin / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; C065096:RNA, ribosomal, 26S