title: ETS-1 transcription factor activates the expression of mouse UDP-Gal:GA2/GM2/GD2/GT2 galactosyltransferase gene.
abstract: UDP-Gal:GA2/GM2/GD2/GT2 galactosyltransferase (Gal-T2) transfers galactose to the terminal N-acetylgalactosamine of either the neutral glycolipid GA2 or of the gangliosides GM2, GD2 and GT2. Previous studies revealed a tight regulation of Gal-T2 activity and mRNA expression during development of the rat CNS. Here, we study in PC12 cells the cis-acting elements involved in the activation of a fragment of 211 bp around the transcription initiation site of the mouse Gal-T2 promoter. Mutagenesis, competition experiments and functional assays showed that the Ets-1 transcription factor is involved in the activation of the Gal-T2 promoter.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Químicas, Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC (UNC-CONICET), Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina.
authors.forename: Lina P
authors.initials: LP
authors.lastname: Raimondi
authors.forename: Jose L
authors.initials: JL
authors.lastname: Daniotti
authors.forename: Hugo J F
authors.initials: HJ
authors.lastname: Maccioni
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016716:PC12 Cells; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D017931:DNA Primers; C494042:Ets1 protein, mouse; C494043:Ets1 protein, rat; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D011994:Recombinant Proteins; D014157:Transcription Factors; D005700:Galactosyltransferases