Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues.
The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.
Kim
Seok H
SH
Department of Pathology, Chungbuk National University College of Medicine, 62 Kaesin-dong, Cheongju, Chungbuk, South Korea.
Kook
Myeong C
MC
Song
Hyung G
HG
D015704:CD4 Antigens / Q000737:chemistry*; D016827:CD8 Antigens / Q000737:chemistry*; D000939:Epitopes; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008198:Lymph Nodes / Q000737:chemistry*; D016612:Paraffin Embedding; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation
D015704:CD4 Antigens; D016827:CD8 Antigens; D000939:Epitopes; D005557:Formaldehyde