Genetic influences on ovulation of primary oocytes in LT/Sv strain mice.
A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.
Everett
Clare A
CA
Medical Genetics, Molecular Medicine Centre, University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.
Auchincloss
Catherine A
CA
Kaufman
Matthew H
MH
Abbott
Catherine M
CM
West
John D
JD
D000818:Animals; D033481:Chromosomes, Mammalian; D005260:Female; D018816:Genes, cdc; D008040:Genetic Linkage; D008540:Meiosis / Q000235:genetics; D008677:Metaphase; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D018895:Microsatellite Repeats; D009865:Oocytes / Q000502:physiology*; D010060:Ovulation / Q000235:genetics*; D013045:Species Specificity; D014960:X Chromosome