[Study on the interface of human hepatocyte L-02 polypropylene:simple culture method of human hepatocyte with spheroidal aggregate culture].
OBJECTIVE
To found new interface of human hepatocyte/poly propylene with good cytocompatibility for made polypropylene hollow fibers bioreactor of bioartificial liver in future.


METHODS
Using the macromolecular hydroperoxide groups on the polypropylene membrane surface as initiators, acrylamides were polymerized on the polypropylene membranes, under induction by both UV irradiation and Fe2+ reduction. Growth characteristics of human hepatocyte L-02 were detected when it was cultured on polystyrene, polypropylene and modified polypropylene membrane surface.


RESULTS
Water contact angle measurement of the polypropylene and the modified polypropylene membranes decreased from (72 +/- 5) degrees to (30 +/- 4) degrees , which indicated that the hydrophilicity of the membrane was improved obviously after the grafting modification. Human hepatocyte L-02 could not adhere and spread on modified polypropylene membrane surface, and grown in spheroidal aggregate with higher density and higher proliferation ratio measured by MTT method.


CONCLUSIONS
Acrylamide polymerized on the polypropylene membranes is a good method which not only improved human hepatocytes cytocompatibility but also found a new simple culture method with spheroidal aggregate culture of human hepatocyte.
Peng
Cheng-hong
CH
Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Han
Bao-san
BS
Gao
Chang-you
CY
Ma
Zu-wei
ZW
Zhao
Zhi-ming
ZM
Wang
Yong
Y
Liu
Hong
H
Zhang
Gui-di
GD
Yang
Mei-juan
MJ
D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D019164:Liver, Artificial; D008567:Membranes, Artificial; D011126:Polypropylenes / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D008567:Membranes, Artificial; D011126:Polypropylenes