Comparison between sodium dodecylsulphate and cetyltrimethylammonium bromide as mobile phases in the micellar liquid chromatography determination of non-steroidal anti-inflammatory drugs in pharmaceuticals.
The retention behaviour of non-steroidal anti-inflammatory drugs (NSAIDs) using micellar mobile phases of sodium dodecylsulphate (SDS) is studied and compared with that observed with micellar mobile phases of cetyltrimethylammonium bromide (CTAB). A liquid chromatographic procedure for the determination of acemetacin, diclofenac, indomethacin, ketoprofen, naproxen and tolmetin in pharmaceutical preparations is described. The proposed system uses a Kromasil C18 analytical column and a solution of 0.15 M SDS at pH 3 with 10% 1-propanol as mobile phase. Under these conditions, the studied NSAIDs elute between 6 and 10 min at a 1 mL min(-1) flow rate. Limits of detection (LOD) are lower than 0.5 microg mL(-1). Recoveries in the analysis of the pharmaceutical preparations are ranged between 91 and 104% respect to the nominal content declared by the manufacturers and the relative standard deviations are in general lower than 4%.
Martínez-Algaba
C
C
Departamento de Química Analítica, Facultad de Farmacia, Universitat de València,C/Vicente Andrés Estellés s/n, E-46100 Burjassot, Valencia, Spain.
Escuder-Gilabert
L
L
Sagrado
S
S
Villanueva-Camañas
R M
RM
Medina-Hernández
M J
MJ
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002138:Calibration; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary; D007202:Indicators and Reagents; D012015:Reference Standards; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002593:Cetrimonium Compounds; D007202:Indicators and Reagents; D012996:Solutions; D012967:Sodium Dodecyl Sulfate; D000077286:Cetrimonium