Double diffusion in gel reactions with antigens insoluble in aqueous media.
Ethanol-soluble, but saline-insoluble antigens were prepared as saline suspensions and studied in double diffusion reactions in a soft agarose gel. Positive reactions were observed with syphilis and SLE sera tested against the Kahn antigen as well as against commercial cardiolipin reagents. Also, ethanol-soluble brain antigen was studied for organ-specific reactions with rabbit immune sera. It was shown that double diffusion in gel can be employed as an analytical procedure for studies on reactions of saline suspensions of ethanol-soluble antigens.
Milgrom
Felix
F
Department of Microbiology and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA. milgrom@buffalo.edu
Loza
Ulana
U
Czechowski
Donna
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D001921:Brain / Q000276:immunology*; D002308:Cardiolipins / Q000276:immunology; D002417:Cattle; D003470:Culture Media; D000431:Ethanol; D005428:Flocculation Tests; D006801:Humans; D005779:Immunodiffusion; D009928:Organ Specificity; D011817:Rabbits; D012995:Solubility; D013552:Swine; D013587:Syphilis / Q000175:diagnosis / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002308:Cardiolipins; D003470:Culture Media; D000431:Ethanol