Map positions of third chromosomal female sterile and lethal mutations of Drosophila melanogaster.
Chromosomal mutations induced by ethyl methanesulfonate (EMS) treatment can cause female sterility or maternal-effect lethality in Drosophila. EMS is particularly useful to researchers because it creates mutations independent of position effects. However, because researchers have little control over the chromosomal site of mutation, post-mutagenic genetic mapping is required to determine the cytological location of the mutation. To make a valuable set of mutants more useful to the research community, we have mapped the uncharacterized part of the female-sterile - maternal-effect lethal Tubingen collection. We mapped 49 female-sterile - maternal-effect lethal alleles and 72 lethal alleles to individual deficiency intervals on the third chromosome. In addition, we analyzed the phenotype of ovaries resulting from female sterile mutations. The observed phenotypes range from tumorous ovaries and early blocks in oogenesis, to later blocks, slow growth, blocks in stage 10, to apparently full development of the ovary. The mapping and phenotypic characterization of these 121 mutations provide the necessary information for the researcher to consider a specific mutant as a candidate for their gene of interest.
Schuetze
Christopher
C
Department of Biology, McGill University, 1205 avenue Docteur Penfield, Montreal, QC H3A 1B1, Canada.
Peters
Michelle
M
Duong
Jia-Jen
JJ
Cavey
Matthieu
M
Dörig
Ruth
R
Lasko
Paul
P
Suter
Beat
B
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D002875:Chromosomes / Q000187:drug effects / Q000235:genetics*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005260:Female; D017344:Genes, Insect / Q000187:drug effects / Q000235:genetics; D005804:Genes, Lethal / Q000187:drug effects / Q000235:genetics*; D007247:Infertility, Female / Q000139:chemically induced / Q000235:genetics*; D009154:Mutation / Q000187:drug effects / Q000235:genetics*; D009866:Oogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D010641:Phenotype
D005020:Ethyl Methanesulfonate