title: Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT.
abstract: Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase in human, has been identified as the rate-limiting factor in telomerase activity, but its detailed mechanism of transcription regulation remains unclear. In this investigation, a novel human gene telomerase transcriptional elements-interacting factor (TEIF) was isolated from HeLa cell based on hTERT promoter yeast one-hybrid assay. TEIF has a 2358-bp open reading frame encoding a predicted protein of 786 amino acids, which is functionally conserved in general eukaryotic species. The transcription of TEIF was detected in cells and expressed an 86-kDa native protein, distributing mainly in cellular nuclei. Its interaction with hTERT promoter was identified and the DNA binding potential was credited to C-terminus of TEIF. Transfection of TEIF into HeLa cells presented marked transactivation of hTERT promoter and stimulated both endogenous hTERT transcription and telomerase activities. These results suggest that TEIF protein might be a transcription regulator and take part in the activation of hTERT.
authors.affiliation: Department of Pathology, Health Science Center of Peking University, Beijing 100083, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Tang
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Fang
authors.initials: F
authors.lastname: Mei
authors.forename: Shaomin
authors.initials: S
authors.lastname: Yang
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Junjie
authors.initials: J
authors.lastname: Lv
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D040461:Gene Components; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D020218:Response Elements; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D004268:DNA-Binding Proteins; C464929:SCYL1 protein, human; D014157:Transcription Factors; D019098:Telomerase