Tissue factor pathway inhibitor and thrombin activatable fibrinolytic inhibitor plasma levels following burn and septic injuries in rats.
Burn and septic injuries induce profound changes in coagulation status. This study examined the changes in plasma tissue factor pathway inhibitor (TFPI) and thrombin activatable fibrinolytic inhibitor (TAFI) levels in a rat model of burn and septic injuries. Rats underwent 30% TBSA cutaneous scald burn injury and septic insult was induced by caecal ligation and puncture (CLP). CLP was superimposed on burn injury to mimic the clinical model of sepsis complicating burn injury. Rats were pretreated with Cprofloxacin orally to colonize their gut with Enterococcus faecalis. TFPI and TAFI plasma levels were measured using functional activity assay kit with a chromogenic method at 24 and 72 hours following the injuries. TFPI levels decreased significantly at 24 hours in burn, CLP, and burn+CLP groups, followed by incomplete rebound recovery at 72 hours in all three groups. On the other hand, TAFI levels increased significantly at 24- and 72-hour time points in all three groups. These results suggest that burn, septic, and their combined injuries perturb coagulation cascade and thrombotic process toward the procoagulant pathway by impairing fibrinolysis.
Ravindranath
Thyyar M
TM
Ronald McDonald Children's Hospital, Maywood, Illinois, USA. travind@lumc.edu
Goto
Masakatsu
M
Demir
Muzaffer
M
Tobu
Mahmut
M
Kujawski
M Florian
MF
Hoppensteadt
D
D
Samonte
V
V
Iqbal
Omer
O
Sayeed
M M
MM
Fareed
J
J
D000818:Animals; D002056:Burns / Q000097:blood* / Q000150:complications; D025901:Carboxypeptidase B2 / Q000097:blood*; D013293:Enterococcus faecalis; D005786:Gene Expression Regulation / Q000502:physiology; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000150:complications / Q000382:microbiology; D019851:Thrombophilia / Q000209:etiology; D013927:Thrombosis / Q000209:etiology; D013997:Time Factors
D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D025901:Carboxypeptidase B2