title: Tissue factor pathway inhibitor and thrombin activatable fibrinolytic inhibitor plasma levels following burn and septic injuries in rats.
abstract: Burn and septic injuries induce profound changes in coagulation status. This study examined the changes in plasma tissue factor pathway inhibitor (TFPI) and thrombin activatable fibrinolytic inhibitor (TAFI) levels in a rat model of burn and septic injuries. Rats underwent 30% TBSA cutaneous scald burn injury and septic insult was induced by caecal ligation and puncture (CLP). CLP was superimposed on burn injury to mimic the clinical model of sepsis complicating burn injury. Rats were pretreated with Cprofloxacin orally to colonize their gut with Enterococcus faecalis. TFPI and TAFI plasma levels were measured using functional activity assay kit with a chromogenic method at 24 and 72 hours following the injuries. TFPI levels decreased significantly at 24 hours in burn, CLP, and burn+CLP groups, followed by incomplete rebound recovery at 72 hours in all three groups. On the other hand, TAFI levels increased significantly at 24- and 72-hour time points in all three groups. These results suggest that burn, septic, and their combined injuries perturb coagulation cascade and thrombotic process toward the procoagulant pathway by impairing fibrinolysis.
authors.affiliation: Ronald McDonald Children's Hospital, Maywood, Illinois, USA. travind@lumc.edu
authors.forename: Thyyar M
authors.initials: TM
authors.lastname: Ravindranath
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Goto
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Demir
authors.forename: Mahmut
authors.initials: M
authors.lastname: Tobu
authors.forename: M Florian
authors.initials: MF
authors.lastname: Kujawski
authors.forename: D
authors.initials: D
authors.lastname: Hoppensteadt
authors.forename: V
authors.initials: V
authors.lastname: Samonte
authors.forename: Omer
authors.initials: O
authors.lastname: Iqbal
authors.forename: M M
authors.initials: MM
authors.lastname: Sayeed
authors.forename: J
authors.initials: J
authors.lastname: Fareed
mesh_terms: D000818:Animals; D002056:Burns / Q000097:blood* / Q000150:complications; D025901:Carboxypeptidase B2 / Q000097:blood*; D013293:Enterococcus faecalis; D005786:Gene Expression Regulation / Q000502:physiology; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000150:complications / Q000382:microbiology; D019851:Thrombophilia / Q000209:etiology; D013927:Thrombosis / Q000209:etiology; D013997:Time Factors
chemical_list: D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor; D025901:Carboxypeptidase B2