title: Intraoperative isolation and processing of BM-derived stem cells.
abstract: To improve tissue regeneration of ischemic myocardium, autologous bone marrow-derived stem cells have been injected intramyocardially in five patients undergoing coronary artery bypass grafting and transmyocardial laser revascularization. An innovative method for the intraoperative isolation of CD133(+)-stem cells in less than 3 hours has been established. After induction of general anesthesia, approx. 60-240 ml of bone marrow were harvested from the posterior iliac crest and processed in the operating room under GMP conditions using the automated cell selection device Clini-MACS. Following standard CABG surgery, LASER channels were shot in predefined areas within the hibernating myocardium. Subsequently, autologous CD133(+)-stem cells (1.9-9.7 x 10(6) cells; purity up to 97%) were injected in a predefined pattern around the laser channels. Through the intraoperative isolation of CD133(+)-cells, this effective treatment of ischemic myocardium can be applied to patients scheduled both for elective and for emergency revascularisation procedures.
authors.affiliation: Department of Cardiovascular and Thoracic Surgery Heinrich-Heine-University of Duesseldorf, Duesseldorf, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ghodsizad
authors.forename: H-M
authors.initials: HM
authors.lastname: Klein
authors.forename: A
authors.initials: A
authors.lastname: Borowski
authors.forename: V
authors.initials: V
authors.lastname: Stoldt
authors.forename: N
authors.initials: N
authors.lastname: Feifel
authors.forename: T
authors.initials: T
authors.lastname: Voelkel
authors.forename: Ch
authors.initials: Ch
authors.lastname: Piechaczek
authors.forename: Er
authors.initials: E
authors.lastname: Burchardt
authors.forename: M
authors.initials: M
authors.lastname: Stockschläder
authors.forename: E
authors.initials: E
authors.lastname: Gams
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007432:Intraoperative Period; D010455:Peptides / Q000032:analysis*; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides