Expression analysis of BCL2L12, a new member of apoptosis-related genes, in colon cancer.
Apoptosis is an active process regulated by a variety of genes. Recently, the molecular cloning, physical mapping and expression analysis of a novel gene of the Bcl-2 family, BCL2L12, was reported. Expression analysis of the BCL2L12 gene in breast cancer confirmed an association of BCL2L12 with favorable prognosis of patients. In the present study, the expression of the BCL2L12 gene was analyzed in colon cancer by RT-PCR. Two transcripts, BCL2L12 and BCL2L12-A, were overexpressed in the cancer tissues as compared to their paired normal mucosa. An association was found between BCL2L12-A transcript expression and nodal status, as well as Dukes' stage. The BCL2L12-A transcript appears to be of importance for colon cancer since its expression is associated with disease progression.
Mathioudaki
Konstantina
K
G. Papanicolaou Research Center of Oncology, Saint Savas Hospital, GR-11522 Athens, Greece.
Scorilas
Andreas
A
Papadokostopoulou
Alexandra
A
Xynopoulos
Dimitrios
D
Arnogianaki
Niki
N
Agnanti
Niki
N
Talieri
Maroulio
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000502:physiology*; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology*; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D006801:Humans; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics; D016016:Proportional Hazards Models; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D018709:Statistics, Nonparametric
C430100:BCL2L12 protein, human; D005819:Genetic Markers; D009124:Muscle Proteins; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2