Anion-exchange displacement centrifugal partition chromatography.
Ion-exchange displacement chromatography has been adapted to centrifugal partition chromatography. The use of an ionic liquid, benzalkonium chloride, as a strong anion-exchanger has proven to be efficient for the preparative separation of phenolic acid regioisomers. Multigram quantities of a mixture of three hydroxycinnamic acid isomers were separated using iodide as a displacer. The displacement process was characterized by a trapezoidal profile of analyte concentration in the eluate with narrow transition zones. By taking advantage of the partition rules involved in support-free liquid-liquid chromatography, a numerical separation model is proposed as a tool for preliminary process validation and further optimization.
Maciuk
Alexandre
A
FRE CNRS 2715 Isolement, Structures, Transformations et Synthèses des Substances Naturelles, IFR 53 Biomolécules, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, F-51687 Reims Cedex 2, France.
Renault
Jean-Hugues
JH
Margraff
Rodolphe
R
Trébuchet
Philippe
P
Zèches-Hanrot
Monique
M
Nuzillard
Jean-Marc
JM