title: Different sensitivities to apoptotic induction by camptothecin between normal and senescent lens epithelial cells.
abstract: OBJECTIVE
  To investigate whether normal and senescent lens epithelial cells have different defense abilities to apoptotic induction factor in vitro.
  
  
  METHODS
  Rabbit lens epithelial cells were cultured, passed. When reaching confluence, cells from the first and seventh passage were stained by x-gal staining to detect cell senescence. Cell apoptosis was detected by TUNEL(Roche). 10 micromol/L camptothecin was used to induce cell apoptosis from the lens epithelial cells of the first and seventh passage to distinguish different sensitivities to apoptotic induction factor between normal and senescent cells.
  
  
  RESULTS
  The senescent cells (41.17% +/- 5.24%) were detected in the lens epithelial cell culture of the seventh passage, which are higher than those of the first passage (0.98% +/- 0.39%). There was no apoptotic cell detected in the cell cultures undisturbed. Exposure of the first passage cells to camptothecin resulted in death of approximately 23.87% +/- 3.45% of the cells during a 36 hour exposure period. In contrast, significantly more lens epithelial cells died through the apoptosis (38.29% +/- 4.01%) from the seventh passage.
  
  
  CONCLUSIONS
  Senescent cells increased with cell passage. Senescence lens epithelial cells do not undergo apoptosis if they were not disturbed. But the vulnerabilities to apoptotic induction between health and senescence cells were different.
authors.affiliation: Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
authors.forename: Haike
authors.initials: H
authors.lastname: Guo
authors.forename: Haiying
authors.initials: H
authors.lastname: Jin
authors.forename: Liya
authors.initials: L
authors.lastname: Wang
authors.forename: Hongyang
authors.initials: H
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology*; D002386:Cataract / Q000209:etiology; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology*; D007908:Lens, Crystalline / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D002166:Camptothecin