[Detection and purification of cytokine secreting T lymphocytes in allogeneic reaction and a preliminary study on its clinical significance].
OBJECTIVE
Detection of cytokine secreting T lymphocytes after allogeneic peripheral blood mononuclear cell (PBMNC) stimulation was carried out and its clinical significance discussed so as to explore a new approach to study allogeneic reactive T lymphocytes.


METHODS
Cytokine secretion assay (CKSA) was applied to detect T lymphocytes secreting interferon gamma (IFNgamma), interleukin-4 (IL-4) and IL-10 at single cell level in human mixed lymphocytes reaction. T cells secreting IFNgamma from PBMNC were detected in patients with acute graft versus host disease (aGVHD).


RESULTS
In comparison with T cells secreting IL-4 and IL-10 which were (0.12 +/- 0.03)% and (0.10 +/- 0.03)%, respectively, a sizable proportion of T cells secreting IFNgamma could be detected (1.12 +/- 0.13)%. Preliminary result indicated that frequency of T cells secreting IFNgamma correlated with the occurrence of aGVHD.


CONCLUSIONS
It is feasible to detect T lymphocytes secreting IFNgamma after allogeneic PBMNC and to apply CKSA technique for clinical research.
Meng
Xiao-jian
XJ
Department of Hematology, the First Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310003, China.
Lin
Mao-fang
MF
D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology
D016207:Cytokines; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma