Evaluation of a colorimetric antifungal susceptibility test by using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride.
A colorimetric antifungal susceptibility test was performed using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. Among 24 strains of Candida species, no trailing growth was found. In 22 and 20 strains, the MICs obtained in the colorimetric assay were within two dilutions of those obtained by the National Committee for Clinical Laboratory Standards method for ketoconazole and itraconazole, respectively.
Shin
Jeong Hwan
JH
Department of Laboratory Medicine, Pusan National University College of Medicine, Seo-Gu Ami-Dong 1-Ga 10, Busan 602-739, Korea.
Choi
Jae-Cheol
JC
Lee
Jeong Nyeo
JN
Kim
Hyung Hoi
HH
Lee
Eun Yup
EY
Chang
Chulhun Ludgerus
CL
D002175:Candida / Q000187:drug effects* / Q000254:growth & development; D003124:Colorimetry; D007202:Indicators and Reagents; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D013778:Tetrazolium Salts
C494145:2,3-diphenyl-5-thienyl-(2)-tetrazolium; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D017964:Itraconazole; D007654:Ketoconazole