[Methods of enumeration of bacteria in drinking water].
Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts (AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%.
Lu
Wei
W
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Wang
Yun
Y
Zhang
Xiao-jian
XJ
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D015169:Colony Count, Microbial / Q000379:methods; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*