title: Accumulation of sulfatide in neuronal and glial cells of arylsulfatase A deficient mice.
abstract: Arylsulfatase A (ASA) degrades sulfatide, seminolipid and lactosylceramide sulfate, glycolipids recognized by the Sulph I antibody although sulfatide is considered the main antigen. Sulfatide is myelin associated but studies have shown a minor distribution also in non-myelin forming cells. The aim of this work was to further study sulfatide in neurons and astrocytes by immunohistochemistry, facilitated by investigation of tissue from adult ASA deficient (ASA -/-) mice. Cells with a low presence of sulfatide might be detected due to lack of ASA activity and accumulation of Sulph I antigens. Sulfatide positive astrocytes and neurons were more numerous and intensely stained in ASA -/- mice, demonstrating a sulfatide accumulation compared to controls. Sulph I staining was especially increased in the molecular layer of cerebellum, in which Purkinje cell dendrites displayed an altered morphology, and in layer IV-VI of cerebral cortex. In hippocampus, immunostaining was found in neuronal cytoplasm in ASA -/- but in nuclear membranes of control mice. We observed a gray matter astrogliosis, which appeared to be associated to sulfatide accumulation. In addition, the developmental change (<20 months) of Sulph I antigens, galactosylceramide, phospholipids and cholesterol were followed by lipid analyses which verified sulfatide and seminolipid accumulation in adult ASA -/- mice, although no lactosylceramide sulfate could be detected. In addition to demonstrating sulfatide in neurons and astrocytes, this study supports the value of ASA -/- mice as a model for metachromatic leukodystrophy and suggests that accumulation of sulfatide beyond myelin might contribute to the pathology of this disease.
authors.affiliation: Institite of Clinical Neuroscience, Experimental Neuroscience Section, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital, SE-431 80 Mölndal, Sweden.
authors.forename: Marie
authors.initials: M
authors.lastname: Molander-Melin
authors.forename: Zarah
authors.initials: Z
authors.lastname: Pernber
authors.forename: Sebastian
authors.initials: S
authors.lastname: Franken
authors.forename: Volkmar
authors.initials: V
authors.lastname: Gieselmann
authors.forename: Jan-Eric
authors.initials: JE
authors.lastname: Månsson
authors.forename: Pam
authors.initials: P
authors.lastname: Fredman
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000166:cytology; D002540:Cerebral Cortex / Q000166:cytology; D002553:Cerebroside-Sulfatase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000737:chemistry* / Q000166:cytology; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D013433:Sulfoglycosphingolipids / Q000032:analysis*
chemical_list: D008055:Lipids; D013433:Sulfoglycosphingolipids; D002553:Cerebroside-Sulfatase