title: [Expression of VEGF in endothelial cells and the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside].
abstract: OBJECTIVE
  To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304) and the inhibitory effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (ST I) in vitro.
  
  
  METHODS
  Exposure to 2.5 mg x L(-1) LPC or LPC + ST I for 24 hours, VEGF protein was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, VEGF mRNA expression in ECV304 was examined by in situ hybridization. VEGF165 mRNA was examined by RT-PCR and Realtime RT-PCR.
  
  
  RESULTS
  LPC upregulated VEGF protein and VEGF mRNA expression in the ECV304 cells. ST I was shown to markedly inhibit the LPC-induced increase of VEGF protein and VEGF165 mRNA (P < 0.001).
  
  
  CONCLUSIONS
  LPC can induce a strong expression of VEGF in ECV304 cells and ST I can inhibit it.
authors.affiliation: Eastern Hepatobiliary Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yao-cheng
authors.initials: YC
authors.lastname: Rui
authors.forename: Yan
authors.initials: Y
authors.lastname: Qiu
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Li
authors.forename: Hou-jia
authors.initials: HJ
authors.lastname: Liu
authors.forename: Wan-sheng
authors.initials: WS
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D005960:Glucosides; D008244:Lysophosphatidylcholines; D012333:RNA, Messenger; D013267:Stilbenes; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A