[mtDNA mutations in mouse tumors].
OBJECTIVE
To investigate variations of mtDNA in mouse tumors and to explore the relationship between mtDNA mutations and murine carcinogenesis.


METHODS
Variations of D-loop, ND3 and tRNAIle + Glu + Met gene fragments of mtDNA from six mouse tumor cell lines were analyzed by PCR-RFLP and PCR-SSCP techniques.


RESULTS
ND3 and tRNAIle + Glu + Met gene fragments of mtDNA from the tumors showed no variations at 27 endonuclease sites. The D-loop of mtDNA from Hca-F demonstrated an additional endonuclease site of Hinf I in contrast to the inbred mouse. Upon PCR-SSCP analysis, the D-loop of mtDNA was found to possess mutations in 4 of 6 tumors.


CONCLUSIONS
D-loop appears to be the hot spot for tumor mtDNA mutations, which may contribute to the carcinogenesis of murine tumors.
Dai
Ji-gang
JG
Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Min
Jia-xin
JX
Zhang
Guo-qiang
GQ
Wei
Hong
H
Xiao
Ying-bin
YB
D000818:Animals; D045744:Cell Line, Tumor; D004272:DNA, Mitochondrial / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D042967:Electron Transport Complex I; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009154:Mutation; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics; D012352:RNA, Transfer, Glu / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D012358:RNA, Transfer, Met / Q000235:genetics
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D011506:Proteins; D012352:RNA, Transfer, Glu; D012355:RNA, Transfer, Ile; D012358:RNA, Transfer, Met; D042967:Electron Transport Complex I; C114961:MT-ND3 protein, human