title: Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism.
abstract: OBJECTIVE
  Polyunsaturated fatty acids (PUFA) deficiency is common in patients with alcoholic liver disease. The suitability of reversing such deficiency remains controversial. The aim was to investigate the role played by PUFA deficiency in the occurrence of alcohol-related mitochondrial dysfunction.
  
  
  METHODS
  Wistar rats were fed either a control diet with or without alcohol (control and ethanol groups) or a PUFA deficient diet with or without alcohol (PUFA deficient and PUFA deficient+ethanol groups). After 6 weeks, liver mitochondria were isolated for energetic studies and fatty acid analysis.
  
  
  RESULTS
  Mitochondria from ethanol fed rats showed a dramatic decrease in oxygen consumption rates and in cytochrome oxidase activity. PUFA deficiency showed an opposite picture. PUFA deficient+ethanol group roughly reach control values, regarding cytochrome oxidase activity and respiratory rates. The relationship between ATP synthesis and respiratory rate was shifted to the left in ethanol group and to the right in PUFA-deficient group. The plots of control and PUFA deficient+ethanol groups were overlapping. Phospholipid arachidonic over linoleic ratio closely correlated to cytochrome oxidase and oxygen uptake.
  
  
  CONCLUSIONS
  PUFA deficiency reverses alcohol-related mitochondrial dysfunction via an increase in phospholipid arachidonic over linoleic ratio, which raises cytochrome oxidase activity. Such deficiency may be an adaptive mechanism.
authors.affiliation: Département d'Hépatologie et Nutrition, CHU, F-14033 Caen cedex, France. piquet-ma@chu-caen.fr
authors.forename: Marie-Astrid
authors.initials: MA
authors.lastname: Piquet
authors.forename: Michel
authors.initials: M
authors.lastname: Roulet
authors.forename: Véronique
authors.initials: V
authors.lastname: Nogueira
authors.forename: Céline
authors.initials: C
authors.lastname: Filippi
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sibille
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hourmand-Ollivier
authors.forename: Marianne
authors.initials: M
authors.lastname: Pilet
authors.forename: Vincent
authors.initials: V
authors.lastname: Rouleau
authors.forename: Xavier M
authors.initials: XM
authors.lastname: Leverve
mesh_terms: D000821:Animal Feed; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D005231:Fatty Acids, Unsaturated / Q000172:deficiency* / Q000494:pharmacology; D008108:Liver Diseases, Alcoholic / Q000178:diet therapy / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated