title: [Expression of PN3 and NaN in trigeminal ganglion during occlusal trauma in rat].
abstract: OBJECTIVE
  To investigate changes of two sodium channels, PN(3) and NaN, during orofacial pain by occlusal trauma in rat.
  
  
  METHODS
  Expressions of PN(3) mRNA and NaN mRNA in trigeminal ganglion were tested during various periods of persistent occlusal trauma with reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  In groups, including control, occlusal trauma groups, PN(3) mRNA and NaN mRNA were all expressed in trigeminal ganglion neurons. In the control group, there were similar density values bilaterally. In the occlusal trauma group, the density values in gel electrophoresis of PN(3) mRNA and NaN mRNA on the intervention side were slightly greater than those on the control side.
  
  
  CONCLUSIONS
  The stimulation of occlusal trauma upregulates expressions of PN(3) mRNA and NaN mRNA, which suggests the signal occurring and conduction of chronic pain by occlusal trauma have the same molecular mechanism of sodium channel as inflammatory pain.
authors.affiliation: Department of Stomatology, General Hospital of PLA, Beijing 100853, China. zhuml@301hospital.com
authors.forename: Mei-ling
authors.initials: ML
authors.lastname: Zhu
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Zuo-qi
authors.initials: ZQ
authors.lastname: Hao
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Duan
mesh_terms: D000818:Animals; D003769:Dental Occlusion, Traumatic / Q000503:physiopathology*; D005157:Facial Pain / Q000209:etiology; D008297:Male; D062559:NAV1.8 Voltage-Gated Sodium Channel; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000096:biosynthesis* / Q000235:genetics; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: D062559:NAV1.8 Voltage-Gated Sodium Channel; D012333:RNA, Messenger; C568319:Scn10a protein, rat; D015222:Sodium Channels