title: Human ribosomal protein S3 interacts with DNA base excision repair proteins hAPE/Ref-1 and hOGG1.
abstract: The human ribosomal protein S3 (hS3) possesses associated activities that suggest alternative roles beyond its participation in protein translation. For example, it is capable of cleaving apurinic/apyrimidinic (AP) DNA via a beta-elimination reaction, an activity that is missing in partially purified extracts of xeroderma pigmentosum group-D fibroblasts. In a recent study, we showed by surface plasmon resonance (SPR) that hS3 also has a very high apparent binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG) and AP sites in DNA. Using the same SPR technology, it is shown here that hS3 positively interacts with the human base excision repair (BER) enzymes N-glycosylase/AP lyase OGG1 and APE/Ref-1. Using a DNA substrate that allows for the detection of 8-oxoG repair, we also show that hOGG1 N-glycosylase activity becomes increasingly more robust in the presence of hS3. Human S3 was found to co-immunoprecipitate with both hOGG1 and APE/Ref-1, indicating that these proteins physically interact with one another. These results raise the possibility that hS3 not only functions as a ribosomal protein but, in addition, may influence repair activities at sites of DNA damage.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana 70808, USA.
authors.forename: Vijay
authors.initials: V
authors.lastname: Hegde
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Walter A
authors.initials: WA
authors.lastname: Deutsch
mesh_terms: D045647:DNA Glycosylases / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D009841:Oligonucleotides / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012269:Ribosomal Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D009841:Oligonucleotides; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D006151:Guanosine; C046215:8-hydroxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase