title: Interaction of echinomycin with guanine: electrochemistry and spectroscopy studies.
abstract: The interaction of antitumor antibiotic, echinomycin (Echi) with guanine (Gua) was thoroughly investigated by adsorptive transfer stripping cyclic voltammetry, ultraviolet and visible adsorption spectra (UV/Vis) and Fourier-transform infrared spectroscopy (FTIR). Electrochemistry provided a simple tool for verifying the occurrence of interaction between Echi and Gua. Echi could be accumulated from the solution and give well-defined electrochemical signals in 0.1 M phosphate buffer solution (pH 7.0) only when Gua was present on the surface of the electrochemically pretreated glass carbon electrode (GCE), suggesting a strong binding of Echi to Gua. All the acquired spectral data showed that a new adduct between Echi and Gua was formed, and two pairs of adjacent intermolecular hydrogen bonds between the Ala backbone atoms in Echi and Gua (Ala-NH to Gua-N3 and Gua-NH2 to Ala-CO) played a dominating role in the interaction. Electrochemistry coupled with spectroscopy techniques could provide a relatively easy way to obtain useful insights into the molecular mechanism of drug-DNA interactions, which should be important in the development of new anticancer drugs with specific base recognition.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
authors.forename: Junguang
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D004448:Echinomycin / Q000737:chemistry*; D004563:Electrochemistry; D006147:Guanine / Q000737:chemistry*; D015394:Molecular Structure; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000903:Antibiotics, Antineoplastic; D004448:Echinomycin; D006147:Guanine