title: Sab (SH3BP5), a novel mitochondria-localized JNK-interacting protein.
abstract: The JNK (c-Jun N-terminal kinase) pathway is activated by diverse stresses and can have an effect on a number of different cellular processes. Protein-protein interactions are critical for efficient signalling from JNK to multiple targets; through a screen for interacting proteins, we identified a novel JNK-interacting protein, Sab (SH3BP5). Sab has previously been found to interact with the Src homology 3 domain of Bruton's tyrosine kinase; however, the interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain. As with c-Jun, the presence of this KIM is essential for Sab to act as a JNK substrate. Interestingly, Sab is associated with the mitochondria and co-localizes with a portion of active JNK after stress treatment. The present study and previously reported work may suggest a possible role for Sab in targeting JNK to this subcellular compartment and/or mediating crosstalk between different signal-transduction pathways.
authors.affiliation: IBLS Division of Biochemistry and Molecular Biology, University of Glasgow, Davidson Building, Glasgow G12 8QQ, UK. c.wiltshire@beatson.gla.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Wiltshire
authors.forename: D A F
authors.initials: DA
authors.lastname: Gillespie
authors.forename: G H W
authors.initials: GH
authors.lastname: May
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D007700:Kinetics; D048670:MAP Kinase Kinase 4; D008928:Mitochondria / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D018384:Oxidative Stress; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; C112230:SH3BP5 protein, human; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D020929:Mitogen-Activated Protein Kinase Kinases