PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-specific primer pair.
A PCR-based assay to identify pandemic group Vibrio parahaemolyticus has been developed. The assay employs an oligonucleotide primer pair derived from the group-specific sequence of an arbitrarily primed-PCR fragment, which is located in the genome encoding a "hypothetical protein," approximately 80% homologous to the Mn2+ and Fe2+ transporter of the NRAMP family of V. vulnificus. The assay distinguished the pandemic group from other V. parahaemolyticus strains by yielding a 235-bp specific amplicon, and can be a useful diagnostic tool for identification of pandemic group strains.
Okura
Masatoshi
M
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, Hyogo, Japan.
Osawa
Ro
R
Iguchi
Atsushi
A
Takagi
Michihiro
M
Arakawa
Eiji
E
Terajima
Jun
J
Watanabe
Haruo
H
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D027682:Cation Transport Proteins; D017931:DNA Primers; D004196:Disease Outbreaks; D014735:Vibrio Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers