title: Serial analysis of gene expression in human keratinocytes and epidermis.
abstract: The demand for large-scale gene expression analysis tools is on the rise now that several genomes have been sequenced. One of these tools, serial analysis of gene expression (SAGE), allows the qualitative as well as quantitative analysis of a large number of genes in a defined tissue or culture model. SAGE has already been successfully used to identify differentially expressed genes in normal physiological processes and pathological conditions. This chapter focuses on the SAGE protocol and its application to cultured human keratinocytes, and on MicroSAGE, an adapted protocol that allows the use of small amounts of mRNA from isolated epidermis or a skin biopsy.
authors.affiliation: Department of Tumor Immunology, Nijmegan Center for Molecular Life Sciences, The Netherlands.
authors.forename: Bastiaan J H
authors.initials: BJ
authors.lastname: Jansen
authors.forename: Gys
authors.initials: G
authors.lastname: de Jongh
authors.forename: Joost
authors.initials: J
authors.lastname: Schalkwijk
authors.forename: Fred
authors.initials: F
authors.lastname: van Ruissen
mesh_terms: D002478:Cells, Cultured; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary