The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres.
This paper investigates the nuclear localization of human telomeres and, specifically, the 4q35 subtelomere mutated in facioscapulohumeral dystrophy (FSHD). FSHD is a common muscular dystrophy that has been linked to contraction of D4Z4 tandem repeats, widely postulated to affect distant gene expression. Most human telomeres, such as 17q and 17p, avoid the nuclear periphery to reside within the internal, euchromatic compartment. In contrast, 4q35 localizes at the peripheral heterochromatin with 4p more internal, generating a reproducible chromosome orientation that we relate to gene expression profiles. Studies of hybrid and translocation cell lines indicate this localization is inherent to the distal tip of 4q. Investigation of heterozygous FSHD myoblasts demonstrated no significant displacement of the mutant allele from the nuclear periphery. However, consistent association of the pathogenic D4Z4 locus with the heterochromatic compartment supports a potential role in regulating the heterochromatic state and makes a telomere positioning effect more likely. Furthermore, D4Z4 repeats on other chromosomes also frequently organize with the heterochromatic compartment at the nuclear or nucleolar periphery, demonstrating a commonality among chromosomes harboring this subtelomere repeat family.
Tam
Rose
R
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
Smith
Kelly P
KP
Lawrence
Jeanne B
JB
D000483:Alleles; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002886:Chromosomes, Human, Pair 17; D002894:Chromosomes, Human, Pair 4 / Q000648:ultrastructure*; D005347:Fibroblasts / Q000378:metabolism; D006570:Heterochromatin / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006579:Heterozygote; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D007399:Interphase; D008840:Microfilament Proteins; D009132:Muscles / Q000166:cytology / Q000378:metabolism; D020391:Muscular Dystrophy, Facioscapulohumeral / Q000235:genetics*; D009154:Mutation; D009687:Nuclear Proteins; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D016615:Telomere / Q000378:metabolism / Q000648:ultrastructure*
C101270:FRG1 protein, human; D006570:Heterochromatin; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins