A PLCdelta1-binding protein, p122RhoGAP, is localized in focal adhesions.
We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-delta(1). Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney cells. In immunoprecipitation studies, p122 co-precipitated with vinculin, indicating that p122 is localized at the sites of focal adhesion. We have also shown that the N-terminal half of p122 is responsible for this localization. It is conceivable, therefore, that p122 is involved in the reorganization of the actin cytoskeleton and focal adhesions that regulate cell-substratum adhesion and cell migration.
Kawai
K
K
Laboratory of Biosignalling, Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Hyogo-ken 978-1297, Japan.
Yamaga
M
M
Iwamae
Y
Y
Kiyota
M
M
Kamata
H
H
Hirata
H
H
Homma
Y
Y
Yagisawa
H
H
D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007668:Kidney / Q000502:physiology; D008954:Models, Biological; D054803:Phospholipase C delta; D011485:Protein Binding; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism*; D016596:Vinculin / Q000502:physiology
D020690:GTPase-Activating Proteins; D007527:Isoenzymes; C085379:rho GTPase-activating protein; D016596:Vinculin; D010738:Type C Phospholipases; D054803:Phospholipase C delta; C518026:Plcd1 protein, rat