title: Effects of alendronate on restoration of biomechanical properties of periodontium in replanted rat molars.
abstract: OBJECTIVE
  We examined the effect of the pretreatment of roots with alendronate on the restoration of the support function of the healing periodontal ligament in replanted rat molars.
  
  
  METHODS
  The left maxillary first molars were extracted, placed in 0.9% NaCl containing 1 mm alendronate (alendronate group) or 0.9% NaCl (control group) for 5 min, and were replanted into their sockets. Groups of animals were killed at 7, 14, and 21 days after replantation. Normal control rats were also killed on the same days. The force required to extract the replanted or normal tooth from its socket was measured, and a load-deformation curve was developed and analyzed. Micro-computed tomography and histologic analyses were also made.
  
  
  RESULTS
  The mechanical properties of the healing periodontal ligament in the alendronate group were gradually restored from 7 to 21 days. However, fractures of the roots and bones during mechanical testing occurred in most of the replanted teeth in the control group at 21 days. The rates of restoration of the mechanical strength, extensibility, stiffness, and toughness for the alendronate group at 21 days were 67, 98, 74, and 68% of the normal controls, respectively. Micro-computed tomography and histologic observations revealed that bone-like structures within the pulp and ankylosis between the roots and socket bones occurred commonly in the control group, but were uncommon in the alendronate group.
  
  
  CONCLUSIONS
  Our findings suggest that the pretreatment with alendronate inhibits the formation of abnormal mineralized tissues and results in better restoration of the support function of the healing periodontal ligament in replanted teeth.
authors.affiliation: Department of Pharmacology, School of Dental Medicine, Tsurumi University, Tsurumi-ku, Yokohama, Japan. shibata-t@tsurumi-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Komatsu
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: S
authors.initials: S
authors.lastname: Shimoda
authors.forename: S
authors.initials: S
authors.lastname: Oida
authors.forename: K
authors.initials: K
authors.lastname: Kawasaki
authors.forename: M
authors.initials: M
authors.lastname: Chiba
mesh_terms: D019386:Alendronate / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D000844:Ankylosis / Q000517:prevention & control; D001696:Biomechanical Phenomena; D016009:Chi-Square Distribution; D003784:Dental Pulp Calcification / Q000517:prevention & control; D003799:Dental Stress Analysis; D008297:Male; D008963:Molar / Q000000981:diagnostic imaging / Q000601:surgery; D010513:Periodontal Ligament / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects; D014057:Tomography, X-Ray Computed; D014082:Tooth Fractures / Q000209:etiology; D014090:Tooth Replantation / Q000009:adverse effects
chemical_list: D019386:Alendronate