title: Effect of prolonged physical exercise on urinary proANP1-30 and proANP31-67.
abstract: Dynamic exercise strongly affects atrial natriuretic peptides (ANP), in particular the mature bioactive alphaANP and the proANP fragments, namely proANP1-98, proANP1-30 and proANP31-67. The proANPs influence kidney functions and their plasma levels increase after physical exercise. We measured urinary proANP1-30 and proANP31-67 levels before and at the end of physical exercise in 28 well-trained male cyclists. For the first time, the proANP1-30 and proANP31-67 urinary levels in athletes before and at the end of a prolonged agonistic bicycle race were measured. Urinary creatinine and total proteins were also measured. The urinary proANP31-67, creatinine and total protein levels were significantly higher at the end of exercise than before. In contrast, proANP1-30/protein and proANP31-67/protein ratios decreased after exercise. Even proANP1-30/creatinine and proANP31-67/creatinine ratios were lower after exercise. A significant correlation between proANP1-30 and proANP31-67 urinary levels at the end of exercise was found. The proANP31-67/creatinine ratio before and after exercise also showed a significant correlation. The variation of urinary proANP fragments confirmed their possible role in physical exercise. In particular, it could be interpreted as a response of the body or kidney to renal impairment occurring during exercise.
authors.affiliation: Clinical Biochemistry Section, Department of Medical Diagnostic Sciences and Spec. Ther., University of Padua, Padua, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Cappellin
authors.forename: Elio F
authors.initials: EF
authors.lastname: De Palo
authors.forename: Rosalba
authors.initials: R
authors.lastname: Gatti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Soldà
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Woloszczuk
authors.forename: Paolo
authors.initials: P
authors.lastname: Spinella
mesh_terms: D000293:Adolescent; D009320:Atrial Natriuretic Factor / Q000652:urine*; D003404:Creatinine / Q000652:urine; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000502:physiology*; D008297:Male; D010446:Peptide Fragments / Q000652:urine*; D011507:Proteinuria; D016482:Urinalysis
chemical_list: D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor; D003404:Creatinine