HIV and hepatitis C virus RNA in seronegative organ and tissue donors.
The objective of our study was to determine whether nucleic acid testing could detect HIV RNA or hepatitis C virus (HCV) RNA in a large series of seronegative organ and tissue donors, and whether this technique should be routinely used to improve viral safety of grafts. We studied 2236 organ donors, 636 tissue donors, and 177 cornea donors. We identified five HCV RNA-positive donors in 2119 HCV-seronegative organ donors, and one HCV RNA-positive donor in 631 HCV-seronegative tissue donors. No HIV-seronegative, HIV RNA-positive donor was identified. Our data suggest that routine nucleic acid testing of organ and tissue donors might increase viral safety in transplantation.
Challine
Dominique
D
Viral Emergency and Organ, Tissue, and Cell Donor Screening Laboratory, Department of Virology (INSERM U635), Hôpital Henri Mondor, Université Paris XII, Créteil, France.
Pellegrin
Bertrand
B
Bouvier-Alias
Magali
M
Rigot
Pierrette
P
Laperche
Liliane
L
Pawlotsky
Jean-Michel
JM
D006678:HIV / Q000302:isolation & purification*; D018023:HIV Seronegativity; D016174:Hepacivirus / Q000302:isolation & purification*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000097:blood*; D014019:Tissue Donors
D018937:Hepatitis C Antibodies; D012367:RNA, Viral