UV spectroscopy and reverse-phase HPLC as novel methods to determine Capreomycin of liposomal fomulations.
Capreomycin (CS) is an antitubercular drug active against several Mycobacterium strains, in particular, against M. Avium. In spite of its activity, it is considered a second line drug because it can induce severe renal and hepatic damages when administered as free drug. However, it is possible to employ drug delivery systems, such as liposomes, to reduce the toxicity of the peptide without loss of its biological activity. For this purpose, appropriately validated time and money saving analytical methods are needed for a careful capreomycin dosage. In the present paper, UV spectroscopy and a reverse-phase HPLC (RP-HPLC) were investigated as alternative methods for capreomycin quantitative analysis. These techniques were validated against the USP XXVI microbiological turbidimetric assay and the normal-phase HPLC (NP-HPLC) method reported in the British Pharmacopoeia 2003. The results obtained showed that either UV spectrophotometry or RP-HPLC are techniques having higher accuracy and reproducibility with respect to the microbiological assay. Moreover, the RP-HPLC method provided improved performances if compared to NP-HPLC. In fact, RP-HPLC showed: (i) enhanced sensitivity and (ii) increased resolution. Thus we propose RP-HPLC and UV as valid alternative methods to the conventional procedures for capreomycin quantitative analysis.
Rossi
Carlo
C
Department of Chimica e Tecnologia del Farmaco, Via del Liceo 1, Università degli Studi di Perugia, 06123 Perugia, Italy.
Fardella
Giuseppe
G
Chiappini
Ione
I
Perioli
Luana
L
Vescovi
Claudia
C
Ricci
Maurizio
M
Giovagnoli
Stefano
S
Scuota
Stefania
S
D000904:Antibiotics, Antitubercular / Q000008:administration & dosage / Q000032:analysis*; D001412:Bacillus subtilis / Q000187:drug effects; D001681:Biological Assay; D002138:Calibration; D002207:Capreomycin / Q000008:administration & dosage / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D007202:Indicators and Reagents; D007711:Klebsiella pneumoniae / Q000187:drug effects; D016014:Linear Models; D008081:Liposomes; D046529:Microscopy, Electron, Transmission; D009391:Nephelometry and Turbidimetry; D010743:Phospholipids; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000904:Antibiotics, Antitubercular; D007202:Indicators and Reagents; D008081:Liposomes; D010743:Phospholipids; D002207:Capreomycin