[The locus coeruleus modulates the inspiratory inhibition induced by electrical stimulation of the Bötzinger complex].
Experiments were done on urethane anesthetized adult rabbits. Long-train electrical stimulation was delivered to the Bötzinger complex (Böt.C) to observe the changes in the peak amplitude of integrated phrenic nerve activity. Then, a long-train electrical stimulation was delivered to the locus coeruleus (LC) or monosodium glutamate was microinjected into the LC . Within a certain period of time, another long-train electrical stimulation was delivered to the Böt.C to observe the responses of phrenic nerve activity. We investigated whether the LC could modulate the inspiratory inhibition induced by electrical stimulation of the Böt.C. The results are as follows: (1) Within a certain period of time after a long-train electrical stimulation applied at the LC, the inspiratory inhibition produced by electrical stimulation at the Böt.C was significantly attenuated. Comparing with the control stimulation that was only delivered at Böt.C without pre-stimulation of the LC, the inspiratory inhibition was decreased by (28.78+/-19.49)%. (2) Similarly, after chemical stimulation of the LC with microinjection of monosodium glutamate, the inspiratory inhibition produced by electrical stimulation of Böt.C was also significantly attenuated [decreased by (19.18+/-8.06)%]. The results obtained suggest that the LC plays a role in the modulation of the inspiratory inhibition of Böt.C stimulation.
Zhang
Fa-Yan
FY
Department of Physiology, Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
Li
Yan-Chun
YC
Xiong
Yi-Qin
YQ
Wang
Gui-Min
GM
Li
Qin
Q
Song
Gang
G
D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D008125:Locus Coeruleus / Q000502:physiology*; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D008839:Microelectrodes; D008845:Microinjections; D009474:Neurons / Q000502:physiology; D010791:Phrenic Nerve / Q000502:physiology*; D011817:Rabbits; D012119:Respiration; D012970:Sodium Glutamate / Q000494:pharmacology; D014520:Urethane / Q000494:pharmacology
D014520:Urethane; D012970:Sodium Glutamate