Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.
This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.
Ch'ng
Toh Hean
TH
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Flood
E Alexander
EA
Enquist
Lynn William
LW
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D019411:Clinical Laboratory Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D011558:Herpesvirus 1, Suid / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000821:virology*; D016716:PC12 Cells; D011557:Pseudorabies / Q000378:metabolism*; D051381:Rats; D017783:Superior Cervical Ganglion / Q000166:cytology