title: HNF1beta/TCF2 mutations impair transactivation potential through altered co-regulator recruitment.
abstract: Mutations in the HNF1beta gene, encoding the dimeric POU-homeodomain transcription factor HNF1beta (TCF2 or vHNF1), cause various phenotypes including maturity onset diabetes of the young 5 (MODY5), and abnormalities in kidney, pancreas and genital tract development. To gain insight into the molecular mechanisms underlying these phenotypes and into the structure of HNF1beta, we functionally characterized eight disease-causing mutations predicted to produce protein truncations, amino acids substitutions or frameshift deletions in different domains of the protein. Truncated mutations, retaining the dimerization domain, displayed defective nuclear localization and weak dominant-negative activity when co-expressed with the wild-type protein. A frameshift mutation located within the C-terminal QSP-rich domain partially reduced transcriptional activity, whereas selective deletion of this domain abolished transactivation. All five missense mutations, which concern POU-specific and homeodomain residues, were correctly expressed and localized to the nucleus. Although having different effects on DNA-binding capacity, which ranged from complete loss to a mild reduction, these mutations exhibited a severe reduction in their transactivation capacity. The transcriptional impairment of those mutants, whose DNA-binding activity was weakly or not affected, correlated with the loss of association with one of the histone-acetyltransferases CBP or PCAF. In contrast to wild-type HNF1beta, whose transactivation potential depends on the synergistic action of CBP and PCAF, the activity of these mutants was not increased by the synergistic action of these two coactivators or by treatment with the specific histone-deacetylase inhibitor TSA. Our findings suggest that the complex syndrome associated with HNF1beta-MODY5 mutations arise from either defective DNA-binding or transactivation function through impaired coactivator recruitment.
authors.affiliation: Biologie du Développement, UMR 7622, CNRS, Université Pierre et Marie Curie, 9 quai St Bernard, 75005 Paris, France.
authors.forename: Elena
authors.initials: E
authors.lastname: Barbacci
authors.forename: Angeliki
authors.initials: A
authors.lastname: Chalkiadaki
authors.forename: Christelle
authors.initials: C
authors.lastname: Masdeu
authors.forename: Cécile
authors.initials: C
authors.lastname: Haumaitre
authors.forename: Ludmilla
authors.initials: L
authors.lastname: Lokmane
authors.forename: Chantal
authors.initials: C
authors.lastname: Loirat
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cloarec
authors.forename: Iannis
authors.initials: I
authors.lastname: Talianidis
authors.forename: Christine
authors.initials: C
authors.lastname: Bellanne-Chantelot
authors.forename: Silvia
authors.initials: S
authors.lastname: Cereghini
mesh_terms: D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019281:Dimerization; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D005819:Genetic Markers; D051539:Hepatocyte Nuclear Factor 1-beta; D006801:Humans; D008297:Male; D009154:Mutation; D010375:Pedigree; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers; C496495:HNF1B protein, human; D014157:Transcription Factors; D051539:Hepatocyte Nuclear Factor 1-beta; D004247:DNA