Investigating mitochondrial radical production using targeted probes.
A range of mitochondria-targeted probe molecules that comprise a lipophilic cation covalently attached to an active moiety have been developed. The lipophilic cation causes the accumulation of these molecules into mitochondria, driven by the mitochondrial membrane potential. To date, we have targeted antioxidants, spin traps, thiol reagents and DNA-alkylating compounds to mitochondria. The selective mitochondrial localization of these compounds enables us to investigate several aspects of the production of reactive oxygen species by mitochondria.
Murphy
M P
MP
Medical Research Council-Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK. mpm@mrc-dunn.cam.ac.uk
D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D007425:Intracellular Membranes / Q000502:physiology; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000502:physiology*; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D008954:Models, Biological; D018384:Oxidative Stress; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species; D014451:Ubiquinone / Q000494:pharmacology; D014807:Vitamin D / Q000494:pharmacology
D000975:Antioxidants; D005609:Free Radicals; D008051:Lipid Bilayers; D017382:Reactive Oxygen Species; D014451:Ubiquinone; D014807:Vitamin D