New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography.
Cryo-electron tomography (cryo-ET) is an emerging imaging technology that combines the potential of three-dimensional (3-D) imaging at molecular resolution (<5 nm) with a close-to-life preservation of the specimen. In conjunction with pattern recognition techniques, it enables us to map the molecular landscape inside cells. The application of cryo-ET to intact cells provides novel insights into the structure and the spatial organization of the cytoskeleton in prokaryotic and eukaryotic cells.
Kürner
Julia
J
Department of Structural Biology, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.
Medalia
Ohad
O
Linaroudis
Alexandros A
AA
Baumeister
Wolfgang
W
D008841:Actin Cytoskeleton / Q000737:chemistry; D000818:Animals; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D003599:Cytoskeleton / Q000737:chemistry*; D005057:Eukaryotic Cells / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D021621:Imaging, Three-Dimensional; D011387:Prokaryotic Cells / Q000737:chemistry / Q000648:ultrastructure*; D014054:Tomography