title: Urinary excretion of nickel after dermal application of Nigerian light crude oil.
abstract: We examined the pattern of excretion of crude oil nickel (CON) in urine and feces. Adult male guinea pigs were treated with a single dose of 5 mL/kg b.w. Nigerian Bonny light crude oil applied on the skin. Untreated guinea pigs served as controls. The CON content was measured spectrophotometrically by the dimethylglyoxime reaction following nitric acid:perchloric acid: sulfuric acid (3:1:1) digestion of blood, urine, and feces samples at 2, 4, 8, and 16 days after the crude oil application. We found a peak of CON excretion in the urine at 2 days and a return to the control level at 16 days. There was no change in the CON content in the feces during the observation time. We found a peak of CON content in the blood at 8 days and a return to control values at 16 days. The implication of these excretion and transport patterns of CON is discussed vis-á-vis the potential application of the urinary nickel content as a valid biochemical indicator of human exposure to environmentally spilled crude oil.
authors.affiliation: Department of Chemistry, Rivers State University of Science and Technology, Nkpolu, Port Harcourt, Rivers State, Nigeria.
authors.forename: Ibiba F
authors.initials: IF
authors.lastname: Oruambo
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D005243:Feces / Q000737:chemistry; D006168:Guinea Pigs; D008297:Male; D009532:Nickel / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D010578:Petroleum / Q000633:toxicity*; D014556:Urine / Q000737:chemistry
chemical_list: D010578:Petroleum; D009532:Nickel