[Tricholoma equestre--animal toxicity study].
Animal toxicity study of Tricholoma equestre mushrooms stored for 12 months at (-)20 degrees C was performed using 30 male BALB/c mice. Three groups of 5 mice each were given suspension of T. equestre powder in water, boiled aqueous extract and chloroform-methanol extract dissolved in Miglyol 812 by gavage for three consecutive days. Mice in control groups were given water, Miglyol 812 and p-phenylenediamine (CAS 106-50-3). Creatine kinase activity was determined in serum collected 72 hours after the final dose. Mean activity of serum creatine kinase in mice treated with T. equestre powder, aqueous extract, chloroform-methanol extract and Miglyol 812 were 157 +/- 93, 129 +/- 30, 96 +/- 38, 111 +/- 66 U/L respectively and did not differ significantly from mean activity in mice which were given water (107 +/- 38 U/L). Mean serum creatine kinase activity in p-phenylenediamine group (265 +/- 63 U/L) was significantly higher than in group treated with water (p<0.01).


CONCLUSIONS
Extracts of Tricholoma equestre mushrooms stored for 12 months at (-)20 degrees C did not cause rhabdomyolysis in male BALB/c mice.
Chodorowski
Zygmunt
Z
I Klinika Chorób Wewnetrznych i Ostrych Zatruć, Akademii Medycznej w Gdańsku. jsanand@amedec.amg.gda.pl
Sznitowska
Małgorzata
M
Wiśniewski
Marek
M
Sein Anand
Jacek
J
Waldman
Wojciech
W
Ronikier
Anna
A
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009145:Mushroom Poisoning; D009183:Mycotoxins / Q000633:toxicity*; D012206:Rhabdomyolysis / Q000097:blood / Q000139:chemically induced; D013997:Time Factors
D009183:Mycotoxins; D003402:Creatine Kinase