title: MicroRNA-143 regulates adipocyte differentiation.
abstract: MicroRNAs (miRNAs) are endogenously expressed 20-24 nucleotide RNAs thought to repress protein translation through binding to a target mRNA (1-3). Only a few of the more than 250 predicted human miRNAs have been assigned any biological function. In an effort to uncover miRNAs important during adipocyte differentiation, antisense oligonucleotides (ASOs) targeting 86 human miRNAs were transfected into cultured human pre-adipocytes, and their ability to modulate adipocyte differentiation was evaluated. Expression of 254 miRNAs in differentiating adipocytes was also examined on a miRNA microarray. Here we report that the combination of expression data and functional assay results identified a role for miR-143 in adipocyte differentiation. miR-143 levels increased in differentiating adipocytes, and inhibition of miR-143 effectively inhibited adipocyte differentiation. In addition, protein levels of the proposed miR-143 target ERK5 (4) were higher in ASO-treated adipocytes. These results demonstrate that miR-143 is involved in adipocyte differentiation and may act through target gene ERK5.
authors.affiliation: Isis Pharmaceuticals, Carlsbad, California 92008, USA. cesau@isisph.com
authors.forename: Christine
authors.initials: C
authors.lastname: Esau
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Kang
authors.forename: Eigen
authors.initials: E
authors.lastname: Peralta
authors.forename: Elaine
authors.initials: E
authors.lastname: Hanson
authors.forename: Eric G
authors.initials: EG
authors.lastname: Marcusson
authors.forename: Lingamanaidu V
authors.initials: LV
authors.lastname: Ravichandran
authors.forename: Yingqing
authors.initials: Y
authors.lastname: Sun
authors.forename: Seongjoon
authors.initials: S
authors.lastname: Koo
authors.forename: Ranjan J
authors.initials: RJ
authors.lastname: Perera
authors.forename: Ravi
authors.initials: R
authors.lastname: Jain
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Dean
authors.forename: Susan M
authors.initials: SM
authors.lastname: Freier
authors.forename: C Frank
authors.initials: CF
authors.lastname: Bennett
authors.forename: Bridget
authors.initials: B
authors.lastname: Lollo
authors.forename: Richard
authors.initials: R
authors.lastname: Griffey
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D020319:Oligodeoxyribonucleotides, Antisense