Molecular weight determination of hypromellose acetate succinate (HPMCAS) using size exclusion chromatography with a multi-angle laser light scattering detector.
The molecular weight of hypromellose acetate succinate (HPMCAS), a polymer used for enteric coating, was determined by means of size exclusion chromatography with a multi-angle laser light scattering detector. The weight-average molecular weight (Mw) of several lots and grades ranged approximately from 17000 to 20000, and the number-average molecular weight (Mn) was around 13000. The inter-day precision of measurement, in terms of the coefficient of variation, was less than 5%.
Fukasawa
Miyuki
M
Specialty Chemicals Research Center, Shin-Etsu Chemical Co. Ltd., 28-1 Nishifukushima, Kubiki-mura, Nakakubiki-gun, Niigata 942-8601, Japan. miyuki-fukasawa@shinetsu.jp
Obara
Sakaé
S
D002850:Chromatography, Gel / Q000379:methods*; D007834:Lasers; D008027:Light; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000032:analysis*; D008970:Molecular Weight; D012542:Scattering, Radiation
C048331:hydroxypropylmethylcellulose acetate succinate; D008747:Methylcellulose