title: Phenotyping the haemostatic system by thrombography--potential for the estimation of thrombotic risk.
abstract: The aim of this paper is to review the thrombogram and its use for phenotyping the haemostatic system. The thrombogram can be readily obtained through Calibrated Automated Thrombography (CAT), using a commercially available fluorometer, dedicated software (Thrombinoscope) and a calibrator. Here we explore the possibility to use platelet-rich plasma (PRP) triggered with a low amount of recombinant human tissue factor (approximately 0.5 pM) and also explore the function of the protein C system by adding activated protein C (APC) or soluble recombinant thrombomodulin (TM). Examples are shown: inherited antithrombin (AT) and protein C deficiencies, and antiphospholipid antibodies.
authors.affiliation: INSERM ERIT-M 0323 et EA 3452, Université Henri Poincaré, Boite Postale 184, 54505 Vandoeuvre-lès-Nancy, France. Veronique.Regnault@nancy.inserm.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Regnault
authors.forename: H Coenraad
authors.initials: HC
authors.lastname: Hemker
authors.forename: Denis
authors.initials: D
authors.lastname: Wahl
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecompte
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology; D001778:Blood Coagulation Disorders / Q000175:diagnosis*; D001792:Blood Platelets / Q000378:metabolism*; D002138:Calibration; D005470:Fluorometry / Q000379:methods*; D006487:Hemostasis; D006801:Humans; D010641:Phenotype; D010949:Plasma / Q000276:immunology*; D011486:Protein C / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D012306:Risk; D012984:Software; D013048:Specimen Handling; D018180:Thrombomodulin / Q000737:chemistry; D013925:Thromboplastin / Q000494:pharmacology; D013927:Thrombosis / Q000473:pathology*; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D011486:Protein C; D011994:Recombinant Proteins; D018180:Thrombomodulin; D013925:Thromboplastin