[Studies on the ploymorphic of sperm of F2 hybrids of red crucian carp x common carp].
AThe ultrastructures of the sperm of F2 hybrids of red crucian carp x common carp were studied by using scanning and transmission electron microscope. The sperm of the F2 hybrids consisted of head, mid-piece and tail. There was no acrosome at the anterior end of the nuclears, whereas there was a vesicle. The results revealed that there existed obviously ploymorphic in the sperm of F2 hybrids. In the water-like semen from males of F2 hybrids, different sizes of the head of the sperm including haploid, diploid, tetraploid, and aneuploid sperm were observed. The head diameter of the smallest sperm was only 1.32 microm, but that of the biggest one was about 18.39 microm, and most of them varied from 1.85 to 2.15 microm. The haploid sperm was normal, while the a-neuploid, diploid, tetraploid and multiploid sperm were abnormal. Among the abnormal sperm, there was a super sperm with about 20 tails, whose head volume was much bigger than that of any other sperm. From the results of the transmission electron microscope, 3 sperm with two nucleus and 1 sperm with two tails were found. This study provided an useful evidence for the mechanism that the formation of tetraploid in F3 hybrids was due to the fertilization of the diploid eggs and diploid sperm produced by F2 hybrids.
Li
Jian Zhong
JZ
Key Lab of Protein Chemistry and Developmental Biology, Ministry of Education, College of Life Science, Hunan Normal University, Changsha 410081, China.
Liu
Shao Jun
SJ
Zhang
Xuan Jie
XJ
Feng
Hao
H
Liu
Yun
Y
D000818:Animals; D002347:Carps / Q000502:physiology*; D002678:Chimera / Q000502:physiology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure*