[Determination of species belonging to the Lactobacillus genus with the use of RAPD-typing].
The numeric analysis of 14 strains of lactobacilli investigated by 57 phenotypical characteristics, has resulted in formation of three clusters: group L. delbrueckii, L. plantarum and the modular group formed by strains of various species. The API-testing, 16S rRNA sequencing and RAPD-typing with primers 5'-AGCAGCGTGG-3' and LP3-ST 5'-TGGTCCGAGC-3' have been carried out for several strains which position in a dendrogram did not correspond to their species diagnosis. The obtained data have allowed the reclassification of the strain L. delbrueckii subsp. bulgaricus B-1923T as L. plantarum B-1923T, L. agilis B-2603 as L. fermentum B-2603, L. amylophilus 30/4/(2) as L. plantarum 30/4/(2). Strain L. confusus 39T, identified by the phenotypical characteristics and data of API-testing as L. fermenturm, was reclassified on the basis of the phylogenetic analysis and RAPD-typing, as L. pentosus 39T.
Lashchevskiĭ
V V
VV
Kovalenko
N K
NK
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D012336:RNA, Ribosomal, 16S