title: Group 3 chromosome bin maps of wheat and their relationship to rice chromosome 1.
abstract: The focus of this study was to analyze the content, distribution, and comparative genome relationships of 996 chromosome bin-mapped expressed sequence tags (ESTs) accounting for 2266 restriction fragments (loci) on the homoeologous group 3 chromosomes of hexaploid wheat (Triticum aestivum L.). Of these loci, 634, 884, and 748 were mapped on chromosomes 3A, 3B, and 3D, respectively. The individual chromosome bin maps revealed bins with a high density of mapped ESTs in the distal region and bins of low density in the proximal region of the chromosome arms, with the exception of 3DS and 3DL. These distributions were more localized on the higher-resolution group 3 consensus map with intermediate regions of high-mapped-EST density on both chromosome arms. Gene ontology (GO) classification of mapped ESTs was not significantly different for homoeologous group 3 chromosomes compared to the other groups. A combined analysis of the individual bin maps using 537 of the mapped ESTs revealed rearrangements between the group 3 chromosomes. Approximately 232 (44%) of the consensus mapped ESTs matched sequences on rice chromosome 1 and revealed large- and small-scale differences in gene order. Of the group 3 mapped EST unigenes approximately 21 and 32% matched the Arabidopsis coding regions and proteins, respectively, but no chromosome-level gene order conservation was detected.
authors.affiliation: Department of Plant Breeding, Cornell University, Ithaca, New York 14853, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Munkvold
authors.forename: R A
authors.initials: RA
authors.lastname: Greene
authors.forename: C E
authors.initials: CE
authors.lastname: Bermudez-Kandianis
authors.forename: C M
authors.initials: CM
authors.lastname: La Rota
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authors.initials: H
authors.lastname: Edwards
authors.forename: S F
authors.initials: SF
authors.lastname: Sorrells
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authors.lastname: Dake
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authors.initials: D
authors.lastname: Benscher
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authors.lastname: Kantety
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authors.lastname: Linkiewicz
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authors.initials: J
authors.lastname: Dubcovsky
authors.forename: E D
authors.initials: ED
authors.lastname: Akhunov
authors.forename: J
authors.initials: J
authors.lastname: Dvorák
authors.lastname: Miftahudin
authors.forename: J P
authors.initials: JP
authors.lastname: Gustafson
authors.forename: M S
authors.initials: MS
authors.lastname: Pathan
authors.forename: H T
authors.initials: HT
authors.lastname: Nguyen
authors.forename: D E
authors.initials: DE
authors.lastname: Matthews
authors.forename: S
authors.initials: S
authors.lastname: Chao
authors.forename: G R
authors.initials: GR
authors.lastname: Lazo
authors.forename: D D
authors.initials: DD
authors.lastname: Hummel
authors.forename: O D
authors.initials: OD
authors.lastname: Anderson
authors.forename: J A
authors.initials: JA
authors.lastname: Anderson
authors.forename: J L
authors.initials: JL
authors.lastname: Gonzalez-Hernandez
authors.forename: J H
authors.initials: JH
authors.lastname: Peng
authors.forename: N
authors.initials: N
authors.lastname: Lapitan
authors.forename: L L
authors.initials: LL
authors.lastname: Qi
authors.forename: B
authors.initials: B
authors.lastname: Echalier
authors.forename: B S
authors.initials: BS
authors.lastname: Gill
authors.forename: K G
authors.initials: KG
authors.lastname: Hossain
authors.forename: V
authors.initials: V
authors.lastname: Kalavacharla
authors.forename: S F
authors.initials: SF
authors.lastname: Kianian
authors.forename: D
authors.initials: D
authors.lastname: Sandhu
authors.forename: M
authors.initials: M
authors.lastname: Erayman
authors.forename: K S
authors.initials: KS
authors.lastname: Gill
authors.forename: P E
authors.initials: PE
authors.lastname: McGuire
authors.forename: C O
authors.initials: CO
authors.lastname: Qualset
authors.forename: M E
authors.initials: ME
authors.lastname: Sorrells
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics*