Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid.
A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR.
Hsiao
Hsien-Yi
HY
Biosensor Laboratory, Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, 136 Chou-Shan Road, Taipei, Taiwan 106, R.O.C.
Tsai
Chih-Cheng
CC
Chen
Suming
S
Hsieh
Bo-Chuan
BC
Chen
Richie L C
RL
D000107:Acetylation; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008962:Models, Theoretical; D010756:Phosphoric Acids / Q000737:chemistry*; D012984:Software; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors; D014466:Ultraviolet Rays
D010756:Phosphoric Acids; D012997:Solvents; D002686:Chitin; D048271:Chitosan; C030242:phosphoric acid