[Study on the interactions between four components in Ligusticum chuanxiong rhizome and acceptors on cardiac muscle membrane].
OBJECTIVE
To study the interactions between four components in Ligusticum chuanxiong rhizome and heart cell membrane acceptors.


METHODS
Through observing the retention characteristics of the four components (tetramethylpyrazine, vanillic, chrysophol, ferulic acid) on cell membrane stationary phase (CMSP) chromatographic column, whether the components combine with cell membrane acceptors was studied, and through further comparing the retention characteristics with those of known activators or antagonists corresponding to cell membrane acceptors, the kind of acceptor with which one component combines was studied.


RESULTS
Tetramethylpyrazine, vanillic and chrysophol had retention on CMSP chromatographic column while ferulic acid hadn't. The retention characteristics of tetramethylpyrazine were similar to activator and antagonist corresponding to a acceptor, vanillic with beta1 acceptor activator.


CONCLUSIONS
Tetramethylpyrazine, vanillic and chrysophol can all combine with cardiac muscle membrane acceptors, while ferulic acid can not; tetramethylpyrazine probably acts on a acceptor and vanillic acts on beta1 acceptor.
Zhang
Yan-ni
YN
Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi' an 710062, China.
Yue
Xuan-feng
XF
Zhang
Zhi-qi
ZQ
D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003373:Coumaric Acids / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D030004:Ligusticum / Q000737:chemistry*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011485:Protein Binding; D011719:Pyrazines / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry; D014641:Vanillic Acid / Q000302:isolation & purification / Q000378:metabolism*
D000880:Anthraquinones; D003373:Coumaric Acids; D011719:Pyrazines; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; C004999:ferulic acid; D014641:Vanillic Acid; C027113:chrysophanic acid; C017953:tetramethylpyrazine