Vesicle swelling regulates content expulsion during secretion.
The involvement of secretory vesicle swelling has been proposed in secretion; however, little is known about its role. Using both the pancreatic acinar cell and neuronal model, we show secretory vesicle swelling in live cells. Our study reveals that vesicle swelling potentiates its fusion at the cell plasma membrane, and is required for expulsion of intravesicular contents. Since the extent of swelling is directly proportional to the amount of vesicular contents expelled, this provides cells with the ability to regulate release of secretory products. These direct observations of the requirement of secretory vesicle swelling in secretion, provides an understanding of the appearance of partially empty vesicles following the process.
Kelly
Marie L
ML
Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA.
Cho
Won Jin
WJ
Jeremic
Aleksandar
A
Abu-Hamdah
Rania
R
Jena
Bhanu P
BP
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D018625:Microscopy, Atomic Force; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010455:Peptides; D051381:Rats; D017207:Rats, Sprague-Dawley; D022142:Secretory Vesicles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D014862:Wasp Venoms / Q000494:pharmacology
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014862:Wasp Venoms; C037500:mastoparan