title: Effects of in vitro storage time and semen-extender on membrane quality of boar sperm assessed by flow cytometry.
abstract: The Norwegian AI company Norsvin has used the short-term semen-extender BTS to extend and store boar semen since the late 1980s. Fertility results have been consistent when extended semen has been used for AI within 3 days after collection, however, from a production and economic point of view it is preferable that semen stored for up to 5 days can be used. The aim of this study was to compare membrane quality of sperm stored in BTS for 3 days with sperm stored in the long-term semen-extenders Androstar, Mulberry III and X-cell for 5 days. Using a split-sample design, plasma membrane- and acrosome-integrity were assessed flow cytometrically by use of Yo-Pro-1 and PNA-FITC, and fluidity and phospholipid asymmetry of the membrane were assessed by use of MC540 and Annexin V-FITC. Due to observed sperm fragmentation in Androstar after Day 1, the data for Androstar were excluded from the analyses. After 5 days of storage, the membrane quality of X-cell-stored sperm was not statistically different from that of sperm stored in BTS for 3 days, while membrane quality of sperm stored in Mulberry III was statistically better on Day 5 compared to BTS on Day 3. In conclusion, Mulberry III and X-cell preserve sperm quality, as well as that of BTS on Day 3, for up to 5 days after collection.
authors.affiliation: Team Semin, P.O. Box 8146 Dep., N-0033 Oslo, Norway. karin.waterhouse@veths.no
authors.forename: K E
authors.initials: KE
authors.lastname: Waterhouse
authors.forename: P M
authors.initials: PM
authors.lastname: De Angelis
authors.forename: T
authors.initials: T
authors.lastname: Haugan
authors.forename: H
authors.initials: H
authors.lastname: Paulenz
authors.forename: P O
authors.initials: PO
authors.lastname: Hofmo
authors.forename: W
authors.initials: W
authors.lastname: Farstad
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D005434:Flow Cytometry; D008297:Male; D008560:Membrane Fluidity; D010743:Phospholipids / Q000032:analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012996:Solutions; D013081:Sperm Motility; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine; D013997:Time Factors
chemical_list: D010743:Phospholipids; D012996:Solutions