title: Non-toxic and short treatment with gemcitabine inhibits in vitro migration of HT-1080 cells.
abstract: Gemcitabine has demonstrated clinical activity in solid tumors. Several in vitro studies were carried out regarding its metabolism, toxicity and cell cycle effects, but none was done on the anti-metastasis potential of the drug. We sought to determine the effect of gemcitabine concentrations on migration velocity of HT-1080 cells at concentrations which do not alter cell cycle progression and proliferation. Cells were treated for 1 or 5 h at IC10-70 of gemcitabine in order to estimate its effects on viability, proliferation and migration capacity using flow cytometry and microscopy imaging, respectively. The gemcitabine treatment for 1 h had no effect on cell proliferation, viability, cycle or migration on HT-1080 cells. Even though the 5 h of exposure at IC10, IC20 and IC50 concentrations did not affect cell viability, proliferation and cell cycle repartition, the mean velocity of HT-1080 dramatically decreased by 50 and 30%, respectively. Gemcitabine at IC70 concentrations for 5 h of exposure first induced a time course inhibition of proliferation, together with a decrease in viability and altered cell morphology, and then inhibited cell migration by 50%. These data suggest the possibility to couple the anti-migratory property of gemcitabine with the known anti-tumoral effect in the treatment of tumors with high metastatic potential.
authors.affiliation: Laboratoire de Dynamique Cellulaire, Ecole Pratique des Hautes Etudes, Grenoble, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Alcouffe
authors.forename: Jean
authors.initials: J
authors.lastname: Boutonnat
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Clément-Lacroix
authors.forename: Mireille
authors.initials: M
authors.lastname: Mousseau
authors.forename: Xavier
authors.initials: X
authors.lastname: Ronot
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D004334:Drug Administration Schedule; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005354:Fibrosarcoma / Q000473:pathology; D005602:France; D006801:Humans; D020128:Inhibitory Concentration 50; D009374:Neoplasms, Experimental / Q000188:drug therapy; D013997:Time Factors; D000093542:Gemcitabine
chemical_list: D003841:Deoxycytidine; D000093542:Gemcitabine