[Polarograhic adsorptive wave of protein hydrolysate in Pb2+ and sodium hydroxide solution and its application].
OBJECTIVE
To propose a new simple and sensitive voltammetric method for determination of proteins.


METHODS
Protein with sulfhydryl or disulfide bond in 0.5 mol x L(-1) NaOH, 1.5 x 10(-4) mol x L(-1) Pb2+ and 0.02% tetrabutylammonium iodide was heated in boiling water for 5 minutes. The reactive product gave a well defined reductive adsorption wave at -0.66 V (vs SCE) by means of single sweep polarography, and the height of derivative wave was proportional to the concentration of proteins.


RESULTS
The peak height was linearly proportional to bovine serum albumin (BSA) or human serum albumin (HSA) concentration in range of 7.5 x 10(-10) -3.0 x 10(-7) mol x L(-1) (r(BSA) = 0.9995, and r(HSA) = 0.9990). The detection limit of BSA or HSA was 3.0 x 10(-10) mol x L(-1). For lysozyme (Lyso), the concentration range was from 1.4 x 10(-8) to 1.3 x 10(-6) mol x L(-10 (r(Lyso) = 0.9997) and the detection limit was 7.0 x 10(-9) mol x L(-1).


CONCLUSIONS
The method is simple, rapid, sensitive and applicable to the assay of diluted human serum albumin samples.
Lan
Jin-Gui
JG
College of Chemistry and Life Science, South Central University for Nationalities, Wuhan 430074, China.
Luo
Deng-Bai
DB
Zhang
Yu-Hua
YH
D000327:Adsorption; D000818:Animals; D006801:Humans; D007854:Lead; D009113:Muramidase / Q000032:analysis; D011048:Polarography / Q000379:methods*; D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D012972:Sodium Hydroxide
D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D007854:Lead; D012972:Sodium Hydroxide; C009405:tetrabutylammonium; D009113:Muramidase