title: Degradation pathway of 2-chloroethanol in Pseudomonas stutzeri strain JJ under denitrifying conditions.
abstract: The pathway of 2-chloroethanol degradation in the denitrifying Pseudomonas stutzeri strain JJ was investigated. In cell-free extracts, activities of a phenazine methosulfate (PMS)-dependent chloroethanol dehydrogenase, an NAD-dependent chloroacetaldehyde dehydrogenase, and a chloroacetate dehalogenase were detected. This suggested that the 2-chloroethanol degradation pathway in this denitrifying strain is the same as found in aerobic bacteria that degrade chloroethanol. Activity towards primary alcohols, secondary alcohols, diols, and other chlorinated alcohols could be measured in cell-free extracts with chloroethanol dehydrogenase (CE-DH) activity. PMS and phenazine ethosulfate (PES) were used as primary electron acceptors, but not NAD, NADP or ferricyanide. Cells of strain JJ cultured in a continuous culture under nitrate limitation exhibited chloroethanol dehydrogenase activity that was a 12 times higher than in cells grown in batch culture. However, under chloroethanol-limiting conditions, CE-DH activity was in the same range as in batch culture. Cells grown on ethanol did not exhibit CE-DH activity. Instead, NAD-dependent ethanol dehydrogenase (E-DH) activity and PMS-dependent E-DH activity were detected.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. John.Dijk@wur.nl
authors.forename: John A
authors.initials: JA
authors.lastname: Dijk
authors.forename: Jan
authors.initials: J
authors.lastname: Gerritse
authors.forename: Gosse
authors.initials: G
authors.lastname: Schraa
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Stams
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D005023:Ethylene Chlorohydrin / Q000378:metabolism*; D009584:Nitrogen; D045542:PQQ Cofactor / Q000378:metabolism; D044223:Pseudomonas stutzeri / Q000254:growth & development / Q000378:metabolism*
chemical_list: D045542:PQQ Cofactor; D005023:Ethylene Chlorohydrin; D000445:Aldehyde Oxidoreductases; C039797:2-chloroacetaldehyde dehydrogenase; D009584:Nitrogen