Increased oxidative DNA damage in mononuclear leukocytes in vitiligo.
Vitiligo is an acquired pigmentary disorder of the skin of unknown aetiology. The autocytotoxic hypothesis suggests that melanocyte impairment could be related to increased oxidative stress. Evidences have been reported that in vitiligo oxidative stress might also be present systemically. We used the comet assay (single cell alkaline gel electrophoresis) to evaluate DNA strand breaks and DNA base oxidation, measured as formamidopyrimidine DNA glycosylase (FPG)-sensitive sites, in peripheral blood cells from patients with active vitiligo and healthy controls. The basal level of oxidative DNA damage in mononuclear leukocytes was increased in vitiligo compared to normal subjects, whereas DNA strand breaks (SBs) were not changed. This alteration was not accompanied by a different capability to respond to in vitro oxidative challenge. No differences in the basal levels of DNA damage in polymorphonuclear leukocytes were found between patients and healthy subjects. Thus, this study supports the hypothesis that in vitiligo a systemic oxidative stress exists, and demonstrates for the first time the presence of oxidative alterations at the nuclear level. The increase in oxidative DNA damage shown in the mononuclear component of peripheral blood leukocytes from vitiligo patients was not particularly severe. However, these findings support an adjuvant role of antioxidant treatment in vitiligo.
Giovannelli
Lisa
L
Department of Preclinical and Clinical Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Firenze, Florence, Italy. lisag@pharm.unifi.it
Bellandi
Serena
S
Pitozzi
Vanessa
V
Fabbri
Paolo
P
Dolara
Piero
P
Moretti
Silvia
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014820:Vitiligo / Q000097:blood*