Expression of PAC1 receptor in rat thymus after irradiation.
In the present work, PAC1-R (G-protein-coupled receptor specific for PACAP) was detected on cells in the normal thymus. Immunohistochemically PAC1-R was expressed strongly in stromal cells of the thymic medulla. Positive cells were also observed in the thymus of fetal and old adult rats. After 8 Gy irradiation to 9-week-old rats, PAC1-R expressions in the thymus decreased and almost recovered by day 21. The expression of PAC1-R mRNA was weak in the thymus and decreased further after irradiation. The expression almost recovered by day 28. Hip and hip/hop variants, which were not expressed in the normal thymus, were expressed in the thymus on days 3, 5 and 21 after irradiation. The expressions of IL-6 and IL-10 tended to increase initially after irradiation then decreased. Histologically, the thymic structures were destroyed on day 3 after irradiation and the thymus almost recovered by day 21. Thus PACAP is thought to be one of the important factors for cross-talk between cells involved in thymic regeneration.
Tokuda
Nobuko
N
Department of Human Science, Yamaguchi University School of Medicine, Ube 755-8505, Japan. toku@yamaguchi-u.ac.jp
Hamasaki
Keiko
K
Mizutani
Noriko
N
Adachi
Yasuhiro
Y
Sawada
Tomoo
T
Funahashi
Hisayuki
H
Shioda
Seiji
S
Fukumoto
Tetsuo
T
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000528:radiation effects; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013950:Thymus Gland / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*
C495505:Adcyap1r1 protein, rat; D018076:DNA, Complementary; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I