title: [Relationship between ATP-binding cassette transporter 1 R219K genetic variation and blood lipids].
abstract: OBJECTIVE
  To investigate whether ATP-binding cassette transporter 1 (ABCA1) R219K genetic variation is correlated with blood lipids.
  
  
  METHODS
  Specimens of peripheral blood were collected from 692 patients with cerebral apoplexy, aged 62 +/- aged 12, and 352 sex- and age-matched persons without cardio-cerebro-vascular disease. Polymerase chain reaction-restricted fragments length polymorphism (PCR-RFLP) was used to determine the ABCA1 genotype: RR type (177 bp), RK type (177 bp, 107 bp, and 70 bp); and KK type (107 bp and 70 bp). The RR and KK type products were sequenced.
  
  
  RESULTS
  The level of HDL-C showed an upward trend in the sequence of RR, RK, and KK genotypes with a significant difference between RR genotype (1.3 mmol/L +/- 0.4 mmol/L) and KK genotype (1.4 mmol/L +/- 0.4 mmol/L), especially in the males. The levels of TG tended downward in the sequence of RR, RK, and KK genotypes, however, without a significant difference between any 2 genotypes. Linear regression analysis showed that the HDL-C level was positively correlated with age in the noncarriers of ABCA1R219K genetic variation (RR genotype), and the TC level was negatively correlated with age in the carriers (RK + KK genotype). In the cohort aged </= 70 the HDL-C level was higher in carriers than in noncarriers.
  
  
  CONCLUSIONS
  ABCA1R219K genetic variation results in a beneficial profile of blood lipids, more evident in males. RK + KK genotype is more pronounced in the individuals aged </= 70.
authors.affiliation: Department of Cardiology, Xiangya Second Hospital, Central South University, Changsha 410011, China.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Zhao
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Xiao
authors.forename: Quan-Zhong
authors.initials: QZ
authors.lastname: Li
authors.forename: Sai
authors.initials: S
authors.lastname: Nie
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Tan
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000368:Aged; D001483:Base Sequence; D002561:Cerebrovascular Disorders / Q000097:blood* / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; D014280:Triglycerides