Determination of avilamycin A and B in pig faeces by solid phase extraction and reverse-phase HPLC assay.
A HPLC method is described for the simultaneous determination of avilamycin A and B in pig faeces, following extraction using acetonitrile and normal-phase solid phase extraction. The HPLC stationary phase was Kromosil 5 micro C-18 with a mobile phase of 48% acetonitrile and 52% 0.01N ammonium acetate buffer, pumped at a flow rate of 1 ml/min. Detection was by UV absorbance at 295 nm and an injection volume of 50 microl was used. Recovery from faeces was >98% and intra-assay precision (CV) was <9.0% for both compounds. The lowest limit of quantification was 0.9 mg/kg (avilamycin A) and 0.2 mg/kg (avilamycin B) with an accuracy of <15% error. No interference was seen from endogenous materials in pig faeces and commonly used veterinary antibiotics.
Sunderland
Julie
J
Bristol Centre for Antimicrobial Research and Evaluation, North Bristol NHS Trust, Southmead Hospital, Bristol, BS105NB, UK. sunderlandj@hotmail.com
Lovering
Andrew M
AM
Tobin
Caroline M
CM
MacGowan
Alasdair P
AP
Roe
John M
JM
Delsol
Anne A
AA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013552:Swine
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; C436976:avilamycin A