Safety and protective effect of a disinfectant (STEL water) for white spot syndrome viral infection in shrimp.
The efficacy of STEL water for protection against white spot syndrome virus (WSSV) infection was evaluated using shrimp. The LC50 of residual chlorine (Cl-) in STEL water for brood-stock and 2-mo-old shrimp were 2.3 and 3.2 ppm, respectively. All 2-month-old shrimp raised in seawater containing more than 40 microl 2l(-1) of a WSSV-infected tissue homogenate died within 3 d post-exposure (dpe). Thus, a 10-fold dose of 400 microl 2 l(-1) was used in the disinfection tests. Low concentrations of STEL water effectively prevented mortality of shrimp at this challenge dose. All 2-month-old shrimp exposed to seawater with 400 microl of viral homogenate disinfected with STEL water at Cl- concentrations over 0.125 ppm for 1 and 10 min, lived until 5 dpe. With 5-mo-old shrimp, all positive control shrimps died within 3 dpe, whereas most shrimp reared in seawater disinfected with STEL water for 1 h before addition of homogenate lived until 5 dpe. Results suggested that continuous disinfection of seawater with STEL water may be effective for preventing WSSV infection in shrimp.
Park
Jong-Hwan
JH
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
Seok
Seung-Hyeok
SH
Cho
Sun-a
SA
Baek
Min-Won
MW
Lee
Hui-Young
HY
Kim
Dong-Jae
DJ
Kim
Han-Yun
HY
Chang
Se-Ok
SO
Park
Jae-Hak
JH
D000818:Animals; D017756:Aquaculture / Q000379:methods*; D003386:Brachyura / Q000821:virology*; D004267:DNA Viruses / Q000187:drug effects* / Q000472:pathogenicity; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006997:Hypochlorous Acid / Q000494:pharmacology; D007928:Lethal Dose 50; D012623:Seawater / Q000821:virology; D013997:Time Factors
D004202:Disinfectants; D006997:Hypochlorous Acid