title: Crystallization and preliminary X-ray analyses of the active and the inactive forms of family GH-8 chitosanase with subclass II specificity from Bacillus sp. strain K17.
abstract: Chitosanase from Bacillus sp. strain K17 (ChoK) belongs to glycoside hydrolase family 8 and exhibits subclass II specificity. The purified protein is structurally stable over a wide pH range (3-10), but is active in a much narrower pH range (4.5-7.5), with optimal activity around pH 6.0. The protein has been successfully crystallized at two different pH values corresponding to the active and inactive states. The crystals diffract to 1.5 and 2.0 A resolution, respectively.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B4 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sakihama
authors.forename: Wataru
authors.initials: W
authors.lastname: Adachi
authors.forename: Shinji
authors.initials: S
authors.lastname: Shimizu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sunami
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukazawa
authors.forename: Mamie
authors.initials: M
authors.lastname: Suzuki
authors.forename: Rie
authors.initials: R
authors.lastname: Yatsunami
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D001407:Bacillus / Q000145:classification / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004789:Enzyme Activation; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration
chemical_list: D006026:Glycoside Hydrolases; C019868:chitosanase