A point mutation in a silencer module reduces the promoter activity for the human mercaptopyruvate sulfurtransferase.
A promoter region of human mercaptopyruvate sulfurtransferase (MST) [EC 2.8.1.2] is G+C-rich and TATA-less, showing features of a house-keeping gene. In the core promoter, a GC box (-284:GGGGCGTGGC:-275) and an initiator (-219:TTATATG:-225) are found. A cap site hunting analysis for human liver cDNA revealed four possible transcriptional start sites, nucleotides -223, -159, -35 and -25. Point mutagenesis and deletion studies suggest that a module of the silencer element is -394:GCTG:-391. A replacement of -391G to C lost the silencer function; on the other hand, a replacement of -394G to T or C, -393C to T or -392T to G markedly reduced the promoter activity.
Nagahara
Noriyuki
N
Department of Environmental Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. noriyuki@nms.ac.jp
Sreeja
V G
VG
Li
Qing
Q
Shimizu
Takako
T
Tsuchiya
Terumasa
T
Fujii-Kuriyama
Yoshiaki
Y
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004742:Enhancer Elements, Genetic; D020862:GC Rich Sequence / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D037941:Silencer Elements, Transcriptional / Q000235:genetics*; D013466:Sulfurtransferases / Q000235:genetics* / Q000378:metabolism; D024363:Transcription Initiation Site / Q000502:physiology
D018076:DNA, Complementary; D013466:Sulfurtransferases; C021550:3-mercaptopyruvate sulphurtransferase