Antibiotic susceptibility of 65 isolates of Burkholderia pseudomallei and Burkholderia mallei to 35 antimicrobial agents.
OBJECTIVE
Fifty isolates of Burkholderia pseudomallei and 15 isolates of Burkholderia mallei were tested for their susceptibilities to 35 antimicrobial agents, including agents not previously tested against these bacteria.


METHODS
MICs were determined by agar dilution in Mueller-Hinton medium.


RESULTS
Among the antibiotics tested, lower MICs were obtained with imipenem, ceftazidime, piperacillin, piperacillin/tazobactam, doxycycline and minocycline. Fluoroquinolones and aminoglycosides had poor activities. A single clinical isolate of B. pseudomallei was resistant to ceftazidime, co-amoxiclav and doxycycline but remained susceptible to imipenem.


CONCLUSIONS
Although B. mallei MICs are often lower, the overall results underline the importance of resistance in both species. The susceptibilities measured are consistent with the current recommendations for the treatment of B. pseudomallei and B. mallei infections.
Thibault
F M
FM
Centre de Recherches du Service de Santé des Armées Emile Pardé, Département de Biologie des Agents Transmissibles, F-38702 La Tronche, France. fthibault@crssa.net
Hernandez
E
E
Vidal
D R
DR
Girardet
M
M
Cavallo
J-D
JD
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001694:Biological Warfare; D042726:Burkholderia mallei / Q000187:drug effects*; D016957:Burkholderia pseudomallei / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D005896:Glanders / Q000382:microbiology; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D006801:Humans; D008554:Melioidosis / Q000382:microbiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D051381:Rats
D000900:Anti-Bacterial Agents