Structure prediction, evolution and ligand interaction of CHASE domain.
Cytokinins are plant hormones involved in the essential processes of plant growth and development. They bind with receptors known as CRE1/WOL/AHK4, AHK2, and AHK3, which possess histidine kinase activity. Recently, the sensor domain cyclases/histidine kinases associated sensory extracellular (CHASE) was identified in those proteins but little is known about its structure and interaction with ligands. Distant homology detection methods developed in our laboratory and molecular phylogeny enabled the prediction of the structure of the CHASE domain as similar to the photoactive yellow protein-like sensor domain. We have identified the active site pocket and amino acids that are involved in receptor-ligand interactions. We also show that fold evolution of cytokinin receptors is very important for a full understanding of the signal transduction mechanism in plants.
Pas
Jakub
J
BioInfoBank Institute, ul. Limanowskiego 24A, 60-744 Poznan, Poland. kuba@bioinfo.pl
von Grotthuss
Marcin
M
Wyrwicz
Lucjan S
LS
Rychlewski
Leszek
L
Barciszewski
Jan
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D003583:Cytokinins / Q000737:chemistry*; D019143:Evolution, Molecular; D008024:Ligands; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D003583:Cytokinins; D008024:Ligands