title: Analysis of urinary nucleosides. IV. Identification of urinary purine nucleosides by liquid chromatography/electrospray mass spectrometry.
abstract: Modified urinary nucleosides are potentially invaluable in cancer diagnosis. High-performance liquid chromatography (HPLC) was combined with full scan mass spectrometry (MS), tandem mass spectrometry and MSn analysis in order to identify purine nucleosides purified from urine. UV peaks evident in the chromatogram were examined by the various mass spectrometric techniques and adenosine, 1-methyladenosine, xanthosine, N1-methylguanosine, N2-methylguanosine, N2,N2-dimethylguanosine, N2,N2,N7-trimethylguanosine, inosine, and 1-methylinosine were each identified in the urine samples from cancer patients. The benefits of the use of LC/MS compared with HPLC alone are discussed.
authors.affiliation: Biomolecular Analysis Mass Spectrometry (BAMS) Facility, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: Edward
authors.initials: E
authors.lastname: Dudley
authors.forename: Filip
authors.initials: F
authors.lastname: Lemière
authors.forename: Walter
authors.initials: W
authors.lastname: Van Dongen
authors.forename: Robin
authors.initials: R
authors.lastname: Tuytten
authors.forename: Salah
authors.initials: S
authors.lastname: El-Sharkawi
authors.forename: A Gareth
authors.initials: AG
authors.lastname: Brenton
authors.forename: Eddy L
authors.initials: EL
authors.lastname: Esmans
authors.forename: Russell P
authors.initials: RP
authors.lastname: Newton
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011684:Purine Nucleosides / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011684:Purine Nucleosides