title: Conservation of aphidicolin-induced fragile sites in Papionini (Primates) species and humans.
abstract: Fragile sites are considered structural features of mammalian chromosomes and a commonly repeated hypothesis is that they are evolutionarily conserved. We tested this hypothesis by establishing the subchromosomal homology of regions harbouring fragile sites in the chromosomes of humans, Macaca fascicularis (MFA) and Mandrillus sphinx (MSP). We delineated the interspecific homology of chromosome bands expressing aphidicolin-induced fragile sites of homologues to human chromosomes 1, 3, 5, 7, 18 and X by the comparative FISH of human BAC and YAC clones. Notably, two YAC clones known to span human chromosome regions containing fragile sites were shown to also span fragile sites in macaques and mandrills. The present comparative BAC/YAC mapping data represent, up to now, the most precise evidence of fragile site conservation during primate evolution.
authors.affiliation: Departament de Biologia Cel.lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, 08193, Barcelona, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Ruiz-Herrera
authors.forename: Francisca
authors.initials: F
authors.lastname: Garcia
authors.forename: Lutz
authors.initials: L
authors.lastname: Frönicke
authors.forename: Montserrat
authors.initials: M
authors.lastname: Ponsà
authors.forename: Josep
authors.initials: J
authors.lastname: Egozcue
authors.forename: Montserrat Garcia
authors.initials: MG
authors.lastname: Caldés
authors.forename: Roscoe
authors.initials: R
authors.lastname: Stanyon
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D043283:Chromosome Fragile Sites; D002874:Chromosome Mapping; D002875:Chromosomes / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008252:Macaca fascicularis; D048528:Mandrillus / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D004791:Enzyme Inhibitors; D005819:Genetic Markers; D016590:Aphidicolin