Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.
Retinaldehyde and retinoic acid are derivatives of vitamin A, and retinaldehyde is the precursor for the synthesis of retinoic acid, a well-known inhibitor of gap junctional intercellular communication. In this investigation, we asked the question if retinaldehyde has similar effects on gap junctions. Gap junctional intercellular communication was measured by scrape-loading and preloading dye-transfer methods, and studies were carried out mainly on cultured liver epithelial cells. Retinaldehyde was found to be a more potent inhibitor (dye transfer reduced by 50% at 2.8 microM) than retinoic acid (dye transfer reduced by 50% at 30 microM) and glycyrrhetinic acid (dye transfer reduced by 50% at 65 microM). Both the 11-cis and all-trans forms of retinaldehyde were equally effective. Retinaldehyde inhibited dye transfer of both anionic Lucifer yellow and cationic Neurobiotin. Inhibition by retinaldehyde developed in less than two minutes at 50 microM, but unlike the reported case with retinoic acid, recovery was slower, though full. In addition to liver epithelial cells, retinaldehyde inhibited gap junctional communication in lens epithelial cells, retinal pigment epithelial cells and retinal ganglion cells.
Pulukuri
Sadhona
S
Eye Research Institute, Oakland University, Rochester, MI 48309-4480, USA.
Sitaramayya
Ari
A
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004396:Coloring Agents / Q000378:metabolism / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D017629:Gap Junctions / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects; D008099:Liver / Q000166:cytology; D051381:Rats; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology; D012172:Retinaldehyde / Q000494:pharmacology*; D013997:Time Factors
D015815:Cell Adhesion Molecules; D004396:Coloring Agents; C070614:neurobiotin; D001710:Biotin; D012172:Retinaldehyde