title: [The study of expression of proliferating cell nuclear antigen and its gene in hypopharyngeal carcinoma].
abstract: OBJECTIVE
  To study the relationship of proliferating cell nuclear antigen (PCNA) and the biological and clinical characteristics of hypopharyngeal carcinoma and its value of clinical prognosis.
  
  
  METHODS
  The SABC-immunohistochemical method and in situ hybridization were correspondingly used to detect the expression of PCNA and its gene in 48 cases of cancer tissue and 15 cases of nearby cancer tissue of primary hypopharyngeal carcinoma. The number of positive cells of PCNA in 63 cases of tissue was represented by proliferating index (PI).
  
  
  RESULTS
  The expression of PCNA correlated with the clinical stage, histological grade and clinical recurrence of hypopharyngeal carcinoma and prognosis of the patients, but did not correlate significantly with tumor location and whether metastasis of cervical lymph nodes or not.
  
  
  CONCLUSIONS
  The expression of PCNA protein may provide a reference in evaluating the malignancy level of hypopharyngeal carcinoma and prognosis of the patients.
authors.affiliation: Department of Otorhinolaryngology, Nanfang Hospital, the First Military Medical University, Guangzhou 510515.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Yao
authors.forename: Xiangping
authors.initials: X
authors.lastname: Li
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger