title: [Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells].
abstract: The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, Chenese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Li
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: You
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Deng
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhao
mesh_terms: D001854:Bone Marrow Cells / Q000502:physiology*; D018106:CD28 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D006801:Humans; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D018106:CD28 Antigens; D016827:CD8 Antigens