[Effect of catechin on bone marrow cell cycle and gene expression of hematopoietic growth factors].
OBJECTIVE
To study the effect of catechin, the active component of Spatholobus suberectus Dunn, on bone marrow cell cycle and the expression of IL-6 and GM-CSF mRNA in spleen cells of normal and marrow-depressed mice in order to clarify the mechanism of hematopoietic-supportive effect of catechin.


METHODS
Flow cytometry was adopted to investigate the influence of catechin on bone marrow cell cycle in mice and the expression of IL-6 and GM-CSF mRNA induced by catechin in spleen cells was detected by RT-PCR technique simultaneously.


RESULTS
The cell proportion of normal and marrow-depressed mice in G0/G1 phase was reduced significantly, while that in S + G2/M phase increased significantly. Being induced by catechin, IL-6 mRNA and GM-CSF mRNA in spleen cells were markedly up-regulated.


CONCLUSIONS
Catechin (2 g x L(-1), intraperitoneally injected to mice daily immediately after irradiation for 7 consecutive days) was shown to promote the expression of IL-6 mRNA and GM-CSF mRNA in spleen cells of mice, through which it can accelerate bone marrow cells of normal mice into cell cycle and help those of marrow-depressed mice to get out of "G1-phase-block", enter into cell cycle and radically accelerate the proliferation and differentiation of hematopoietic stem cell/hematopoietic progenitor cell (HSC/HPC).
Liu
Ping
P
Research Institute for Clinical Pharmacology, General Hospital of PLA, Beijing 100853, China. lp7211@nsfc.gov.cn
Wang
Dong-xiao
DX
Chen
Ruo-yun
RY
Chen
Meng-li
ML
Yin
Jian-fen
JF
Chen
Gui-yun
GY
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D015850:Interleukin-6; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002392:Catechin