title: Cytoprotective effect on oxidative stress and inhibitory effect on cellular aging of Uncaria sinensis Havil.
abstract: The ethanol extract from the hooks and stems of Uncaria sinensis Havil (Rubiaceae) exhibited significant inhibitory activity on oxidative stress and the age-dependent shortening of the telomeric DNA length. In the peroxidation model using t-BuOOH, the Uncaria sinensis extract showed a notable cytoprotective effect on the HEK-N/F cells with 65.0 +/- 3.0% of cell viability when compared with control cells at a concentration of 50 microg/ml. In addition, the Uncaria sinensis extract exhibited a significant cytoprotective effect against UVB-induced oxidative damage. The life-span of the HEK-N/F cells was elongated by 201% as a result of the continuous administration of 3 microg/ml of the Uncaria sinensis extract compared to that of the control. These observations were attributed to the inhibitory effect of the Uncaria sinensis extract on the age-dependent shortening of the telomere length as shown by the Southern blots of the terminal restriction fragments (TRFs) of DNA extracted from subculture passages.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Minkyun
authors.initials: M
authors.lastname: Na
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kamiryo
authors.forename: Yu-ichiro
authors.initials: Y
authors.lastname: Senoo
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yokoo
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Miwa
authors.forename: Kyung Sik
authors.initials: KS
authors.lastname: Song
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Seong
mesh_terms: D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems; D032070:Uncaria
chemical_list: D010936:Plant Extracts