Simultaneous determination of magnesium lithospermate B, rosmarinic acid, and lithospermic acid in beagle dog serum by liquid chromatography/tandem mass spectrometry.
A rapid, sensitive and specific isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the simultaneous quantitative determination of magnesium lithospermate B (MLB), rosmarinic acid (RA), and lithospermic acid (LA) in beagle dog serum, with silibinin as internal standard. The serum samples were treated by liquid-liquid extraction and analyzed using LC/MS/MS with a TurboIonSpray source. A short run-time (3 min) fulfilled the need for monitoring serum levels of MLB, RA, and LA in large-scale studies. The calibration curves for MLB, RA, and LA were linear over the ranges 8-2048, 4-1024, and 4-1024 ng/mL, respectively, with coefficients of correlation >0.999. The intra- and inter-day precision (CV) of analysis was <10%, and accuracy ranged from 90-104%. This quantitation method was successfully applied to a pharmacokinetic study of salvianolate administrated by intravenous infusion with dosage of 6 mg/kg in beagle dogs.
Li
Xiaochuan
X
Department of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
Yu
Chen
C
Sun
Weikang
W
Liu
Gangyi
G
Jia
Jingying
J
Wang
Yiping
Y
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001572:Benzofurans / Q000097:blood*; D002109:Caffeic Acids / Q000008:administration & dosage / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000097:blood*; D053630:Depsides; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D016166:Free Radical Scavengers / Q000032:analysis / Q000378:metabolism; D007275:Injections, Intravenous; D007773:Lactates / Q000008:administration & dosage / Q000493:pharmacokinetics; D010618:Phenanthrolines / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000097123:Rosmarinic Acid
D000975:Antioxidants; D001572:Benzofurans; D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D007773:Lactates; D010618:Phenanthrolines; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C061091:lithospermate B; C028972:dan-shen root extract; C066201:salvianolic acid A