[Study on GC-MS fingerprint analysis in rhizome of volatile oil of Acorus tatarinowii].
OBJECTIVE
To establish the method of fingerprint analysis on volatile oil in rhizome of Acorus tatarinowii by GC-MS, and to study the main characteristic components.


METHODS
The main components of 10 samples were determined by GC-MS.


RESULTS
The injector temperature was 250 degrees C. The interface temperature was 230 degrees C. The column flow was 1.3 mL x min(-1). The column pressure was 80 kPa. The detector volt was 1.4 kV. The temperature rate was 3 degrees C x min(-1). And the main characteristic components were composed of the methyleugenol (2.13%), cis-methylisoeugenol (4.48%), trans-methylisoeugenol (0.82%), gamma-asarone (4.51%), beta-asarone (66.15%), alpha-asarone (6.35%). And the RSD of precision and reproducibility and stability was almost in the range of 5%.


CONCLUSIONS
The method is reliable, accurate and can be used for fingerprint analysis of volatile oil of Acorus tatarinowii.
Wei
Gang
G
The frist Affiliated Hospital of Guangzhou University of Treditional Chinese Medicine, Guangzhou 510405, China. weigang021@163.com
Fang
Yong-Qi
YQ
Liu
Dong-Hui
DH
Lin
Shuang-Feng
SF
D029023:Acorus / Q000737:chemistry*; D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000032:analysis; D005054:Eugenol / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D009822:Oils, Volatile; C012195:asarone; C005223:methyleugenol; D005054:Eugenol; C031050:isomethyleugenol