title: Determination of lipid oxidation level in broiler meat by liquid chromatography.
abstract: An assay was conducted for the determination of malondialdehyde (MDA) levels in broiler meat. The method involves extraction of tissues with trichloroacetic acid (TCA) and reaction of the TCA extract with 2,4-dinitrophenylhydrazine (DNPH). After separation of the MDA-DNPH complex using a solid-phase extraction C18 column, samples were eluted with 1 mL acetonitrile. Aliquots of 20 microL acetonitrile were analyzed by liquid chromatography on reversed-phase C18 column (3 microm) with UV detection. The products were eluted isocratically with the mobile phase containing acetonitrile-water-acetic acid (39 + 61 + 0.2, v/v/v). The retention time for MDA-DNPH was 6.5 min, and the detection limit was 3.5 microg/kg. Two extraction methods (cold and hot) were also used in the study. The results showed that hot extraction increased results about 55.8% and recovery from samples spiked with 116.6 microg/kg was lower (74.6%) in comparison with cold extraction (94.7%).
authors.affiliation: University of Veterinary Medicine, Department of Food Hygiene and Technology, Komenského 73, 041 81 Kosice, The Slovak Republic. marcincak@lycos.com
authors.forename: Slavomir
authors.initials: S
authors.lastname: Marcincák
authors.forename: Jozef
authors.initials: J
authors.lastname: Sokol
authors.forename: Pavel
authors.initials: P
authors.lastname: Bystrický
authors.forename: Peter
authors.initials: P
authors.lastname: Popelka
authors.forename: Peter
authors.initials: P
authors.lastname: Turek
authors.forename: Mangesh
authors.initials: M
authors.lastname: Bhide
authors.forename: Dionýz
authors.initials: D
authors.lastname: Máté
mesh_terms: D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D050356:Lipid Metabolism; D008315:Malondialdehyde / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D010084:Oxidation-Reduction
chemical_list: D008315:Malondialdehyde