title: YY1 DNA binding and PcG recruitment requires CtBP.
abstract: We found that mammalian Polycomb group (PcG) protein YY1 can bind to Polycomb response elements in Drosophila embryos and can recruit other PcG proteins to DNA. PcG recruitment results in deacetylation and methylation of histone H3. In a CtBP mutant background, recruitment of PcG proteins and concomitant histone modifications do not occur. Surprisingly, YY1 DNA binding in vivo is also ablated. CtBP mutation does not result in YY1 degradation or transport from the nucleus, suggesting a mechanism whereby YY1 DNA binding ability is masked. These results reveal a new role for CtBP in controlling YY1 DNA binding and recruitment of PcG proteins to DNA.
authors.affiliation: University of Pennsylvania, School of Veterinary Medicine, Department of Animal Biology, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Srinivasan
authors.forename: Michael L
authors.initials: ML
authors.lastname: Atchison
mesh_terms: D000429:Alcohol Oxidoreductases; D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D050981:Erythroid-Specific DNA-Binding Factors; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1; D063151:Polycomb Repressive Complex 2; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D050986:YY1 Transcription Factor
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D006657:Histones; D009687:Nuclear Proteins; C066770:Pc protein, Drosophila; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; D004247:DNA; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein; C083329:E(z) protein, Drosophila; D063151:Polycomb Repressive Complex 2; D063150:Polycomb Repressive Complex 1