title: Analysis of HOX homeodomain proteins and gene transcripts in the epidermis.
abstract: HOX homeodomain proteins are thought to be master developmental regulators of tissue patterning during embryogenesis. These DNA binding proteins also have diverse roles in adult cell function, and derangement of HOX genes has been associated with several types of cancer. In this chapter we present protocols for the immunohistochemical localization of HOX proteins in the epidermis. We also provide in situ hybridization protocols for detection of HOX gene mRNA transcripts in the epidermis.
authors.affiliation: Department of Dermatology, VA Medical Center, University of California at San Francisco, USA.
authors.forename: László G
authors.initials: LG
authors.lastname: Kömüves
authors.forename: Corey
authors.initials: C
authors.lastname: Largman
mesh_terms: D000818:Animals; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; D012333:RNA, Messenger