[Study on the expression and mutation of cadherin 6 gene in human ovarian carcinoma].
OBJECTIVE
To study expression and mutation of cadherin 6 gene in human ovarian tumor tissues and its clinical significance and to explore new molecular biomarker of ovarian tumors.


METHODS
The expression and mutation of cadherin 6 in 41 cases of malignant ovarian tumors, 15 cases of benign ovarian tumors and borderline ovarian tumors and 17 cases of normal tissues were detected by RT-PCR and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique respectively.


RESULTS
The positive expression rates of cadherin 6 were 71% in normal group, 53% in benign ovarian tumors, 24% in malignant ovarian tumors, being significantly higher in the former two groups than in malignant tumor group (P < 0.05). Statistical analysis revealed a significant correlation between cadherin 6 gene expression and clinical stages and histological subtype of malignant ovarian tumors. Lower expression rate of cadherin 6 gene was found in cancer tissues at stages III approximately IV than at stages I approximately II (P < 0.01). No mutation was found in normal group and benign tumor tissues and it was observed in 2 out of 41 malignant ovarian tumor tissues.


CONCLUSIONS
These results suggest that expression of cadherin 6 gene tends to lack in advanced ovarian carcinoma with lymph node metastases, as well as in poorly differentiated carcinomas. It may be a prognostic marker of ovarian carcinoma.
Li
Dan-rong
DR
Department of Basic Science, Cancer Institute of Guangxi Zhuang Automomous Region, Nanning 530021, China.
Li
Li
L
Zhang
Wei
W
Wang
Li-mei
LM
Zhang
Jie-qing
JQ
D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D009154:Mutation; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015820:Cadherins; D012333:RNA, Messenger