[Effect of 103palladium radioactive stent on expression of smooth muscle actin in bile duct during healing process and its significance].
OBJECTIVE
To observe the effect of radiation on the expression of smooth muscle actin (SMA) in the bile duct during the healing process and the inhibitory function of (103)palladium (Pd) radioactive stent on the stricture of bile duct after injury.


METHODS
Twelve mongrel dogs were made models of bile duct injury: duodenotomy was performed, a balloon catheter was inserted into the general bile duct and saline with high pressure was perfused thereinto to cause laceration of the mucosa, and then the balloon catheter was withdrawn and ordinary alloy stent or (103)Pd radioactive stent was inserted into the general bile duct. Thirty days after the dogs were killed. Their bile ducts were taken out to undergo HE staining to observe the area of general bile duct, thickness of the tunica intima, area of residual bile duct cavity, stricture degree, and circumference of bile duct. The expression of SMA in the bile duct tissue was detected by immunoistochemistry.


RESULTS
SMA was expressed in 5 of the 6 specimens of bile duct in the (103)Pd radioactive stent group and 2 of the 6 specimens of the ordinary stent group (P < 0.01). The maximum thickness of tunica intima of general bile duct was 0.78 mm +/- 0.12 mm in the (103)Pd radioactive stent group, significantly less than that of the ordinary stent group (1.86 mm +/- 0.14 mm, P < 0.01). The percentage of maximum stricture area of the (103)Pd radioactive stent group was 23% +/- 16%, significantly lower that that of the ordinary stent group (56% +/- 22%, P < 0.01). The circumference of bile duct cavity of the (103)Pd radioactive stent group was 9.7 mm +/- 1.6 mm, significantly longer that of the ordinary stent group (7.0 mm +/- 1.4 mm, P < 0.01).


CONCLUSIONS
(103)Pd radioactive stent reduces the expression of SMA in the bile duct during the healing process, thus inhibiting the stricture of bile duct caused by scar contracture at the anastomotic stoma.
He
Gui-Jin
GJ
Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China.
Wang
Ya-Zhu
YZ
Xu
Shu-He
SH
Gao
Hong
H
Jiang
Tao
T
Dai
Xian-Wei
XW
Ma
Kai
K
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001652:Bile Ducts / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D003251:Constriction, Pathologic / Q000517:prevention & control / Q000628:therapy; D004285:Dogs; D008297:Male; D009130:Muscle, Smooth / Q000528:radiation effects; D010165:Palladium / Q000008:administration & dosage*; D011868:Radioisotopes / Q000008:administration & dosage*; D015607:Stents; D014945:Wound Healing / Q000528:radiation effects
D000199:Actins; D011868:Radioisotopes; D010165:Palladium