Carbonic anhydrase III is not required in the mouse for normal growth, development, and life span.
Carbonic anhydrase III is a cytosolic protein which is particularly abundant in skeletal muscle, adipocytes, and liver. The specific activity of this isozyme is quite low, suggesting that its physiological function is not that of hydrating carbon dioxide. To understand the cellular roles of carbonic anhydrase III, we inactivated the Car3 gene. Mice lacking carbonic anhydrase III were viable and fertile and had normal life spans. Carbonic anhydrase III has also been implicated in the response to oxidative stress. We found that mice lacking the protein had the same response to a hyperoxic challenge as did their wild-type siblings. No anatomic alterations were noted in the mice lacking carbonic anhydrase III. They had normal amounts and distribution of fat, despite the fact that carbonic anhydrase III constitutes about 30% of the soluble protein in adipocytes. We conclude that carbonic anhydrase III is dispensable for mice living under standard laboratory husbandry conditions.
Kim
Geumsoo
G
Laboratories of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-0812, USA.
Lee
Tae-Hoon
TH
Wetzel
Petra
P
Geers
Cornelia
C
Robinson
Mary Ann
MA
Myers
Timothy G
TG
Owens
Jennie W
JW
Wehr
Nancy B
NB
Eckhaus
Michael W
MW
Gros
Gerolf
G
Wynshaw-Boris
Anthony
A
Levine
Rodney L
RL
D000818:Animals; D024403:Carbonic Anhydrase III / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D018390:Gene Targeting; D048788:Growth and Development; D066298:In Vitro Techniques; D008136:Longevity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000201:enzymology / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress
D024403:Carbonic Anhydrase III