Plasma F2-isoprostanes and coronary artery calcification: the CARDIA Study.
BACKGROUND
Oxidation of lipids in lipoproteins and cells may initiate and enhance the early development of cardiovascular disease.


RESULTS
We assayed F(2)-isoprostanes, oxidation products of arachidonic acid, by gas chromatography-mass spectrometry in a biracial cohort of 2850 young healthy adult men and women. Coronary artery calcification (CAC), a component of coronary artery atherosclerosis, was detectable in 10% of the cohort and appeared to be in its initial stages (Agatston scores <20 in 47% and <100 in 83% of CAC-positive participants). After adjusting for sex, clinical site, age, and race, the presence of any CAC was 24% more likely among those with high vs low concentrations of F(2)-isoprostanes [odds ratio (OR) = 1.24 per 92.2 pmol/L (32.7 ng/L; 1 SD of F(2)-isoprostanes); 95% confidence interval (CI), 1.09-1.41]. The OR was only slightly attenuated [1.18 per 92.2 pmol/L (32.7 ng/L); CI, 1.02-1.38] after further adjustment for body mass index, smoking, serum lipids, C-reactive protein, antioxidant supplementation use, diabetes, and blood pressure. As a continuous variable, the Agatston score increased by 6.9% per 92.2 pmol/L (32.7 ng/L) of F(2)-isoprostane concentration (P <0.01). Whereas CAC prevalence was lower in women than men, mean (SD), F(2)-isoprostanes were higher in women {190 (108.9) pmol/L [67.4 (38.6) ng/L]} than in men {140.4 (55.6) pmol/L [49.8 (19.7) ng/L]}. Nevertheless, F(2)-isoprostanes were associated with an increased risk of CAC in both sexes.


CONCLUSIONS
This association between increased concentrations of circulating F(2)-isoprostanes and CAC in young healthy adults supports the hypothesis that oxidative damage is involved in the early development of atherosclerosis.
Gross
Myron
M
Department of Laboratory Medicine and Pathology, School of Medicine, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA. gross@epi.umn.edu
Steffes
Michael
M
Jacobs
David R
DR
Yu
Xinhua
X
Lewis
Linda
L
Lewis
Cora E
CE
Loria
Catherine M
CM
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002114:Calcinosis / Q000209:etiology*; D003324:Coronary Artery Disease / Q000209:etiology* / Q000473:pathology; D003331:Coronary Vessels / Q000473:pathology*; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008955:Models, Cardiovascular; D012737:Sex Factors
D028441:F2-Isoprostanes