[Effects of advanced glycation end products and hypoxia on endothelin-1 secretion by bovine retinal microvascular pericytes].
OBJECTIVE
To investigate the effects of advanced glycation end products(AGE) and hypoxia on endothelin-1 (ET-1) secretion by cultured bovine retinal microvascular pericytes (BRPs).


METHODS
BRPs were incubated with different concentrations of AGE (8, 32, 125 microg/ ml) for four days, hypoxia (10% O2, 5% CO2, 85% N2) for 12, 24, 48 hours, respectively and AGE for 2 days firstly and then hypoxia for 48 hours. Finally, ET-1 production was assessed in culture supernatants of BRPs under basal and stimulated conditions by radioimmunoassay.


RESULTS
Hypoxia stimulated ET-1 production in a time-dependent manner in cultured BRPs. AGE could not induce the production significantly, but it could upregulate the effect of hypoxia on ET-1.


CONCLUSIONS
These results suggested that AGE and hypoxia could regulate the ET-1 production in BRPs. ET-1 might be related to microvascular dysfunction in diabetes.
Chen
Baihua
B
Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
Jiang
Deyong
D
Tang
Luosheng
L
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D000860:Hypoxia; D008833:Microcirculation / Q000503:physiopathology; D020286:Pericytes / Q000187:drug effects* / Q000378:metabolism; D012171:Retinal Vessels / Q000166:cytology* / Q000187:drug effects / Q000503:physiopathology
D019332:Endothelin-1; D017127:Glycation End Products, Advanced