Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus.
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target.
Moreno
Renata
R
Centro de Biología Molecular Severo Ochoa CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.
Hidalgo
Aurelio
A
Cava
Felipe
F
Fernández-Lafuente
Roberto
R
Guisán
José Manuel
JM
Berenguer
José
J
D000332:Aerobiosis; D000693:Anaerobiosis; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007613:Kanamycin Resistance / Q000235:genetics; D008345:Manganese / Q000378:metabolism*; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*
D016372:RNA, Antisense; D012333:RNA, Messenger; D008345:Manganese; D002374:Catalase