title: Description of a novel HLA-B allele, B*5613, identified during HLA-typing using sequence-specific oligonucleotide hybridization and sequence-specific amplification.
abstract: Here, we report on the characterization of a novel human leukocyte antigen (HLA)-B allele, B*5613. The allele was identified in an adult male from North Africa who was suffering from sickle cell anemia. HLA-B*5613 most closely matches to B*5601 differing only by a substitution of three nucleotides of codon 180. Due to this substitution, low-resolution HLA-typing using sequence-specific oligonucleotide hybridization or amplification using sequence-specific primers gave inconclusive results. DNA sequencing confirmed a variation of codon 180 (CTG-->GAC) resulting in an amino acid substitution Leu156Asp.
authors.affiliation: Institute of Transfusion Medicine, Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Germany. berthold.hoppe@charite.de
authors.forename: B
authors.initials: B
authors.lastname: Hoppe
authors.forename: G A
authors.initials: GA
authors.lastname: Heymann
authors.forename: C
authors.initials: C
authors.lastname: Schoenemann
authors.forename: M
authors.initials: M
authors.lastname: Nagy
authors.forename: H
authors.initials: H
authors.lastname: Kiesewetter
authors.forename: A
authors.initials: A
authors.lastname: Salama
mesh_terms: D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens; C497985:HLA-B*56:13 antigen; C056079:HLA-B56 antigen