title: Transmission of Streptococcus mutans in a group of Turkish families.
abstract: OBJECTIVE
  To investigate the transmission of Streptococcus mutans in a group of Turkish families using AP-polymerase chain reaction (PCR) detection.
  
  
  METHODS
  Eight mothers who had high S. mutans levels in unstimulated saliva and 8 children aged between 2 and 3 years participated in the study. Plaque samples from each child were collected with the tips of sterile toothpicks for S. mutans counts. Although not part of the original study design, S. mutans samples were also obtained from the unstimulated saliva of the three fathers who shared the same households. Three typical isolates of S. mutans were isolated from TYCSB agar of each subject and identified by sugar fermentation tests. S. mutans ATCC 10449 was used as the reference strain. AP-PCR was conducted with OPA-05 primer.
  
  
  RESULTS
  All of the mothers and fathers shared the similar genotypes within their children. The fathers also harbored similar genotypes to their spouses.
  
  
  CONCLUSIONS
  The mothers or the fathers could be the source for the transmission of S. mutans to their children.
authors.affiliation: Ege University Dental Faculty, Department of Pedodontics, 35100 Bornova-Izmir, Turkey. nazan@dent.ege.edu.tr
authors.forename: N K
authors.initials: NK
authors.lastname: Ersin
authors.forename: E H
authors.initials: EH
authors.lastname: Kocabas
authors.forename: A R
authors.initials: AR
authors.lastname: Alpoz
authors.forename: A
authors.initials: A
authors.lastname: Uzel
mesh_terms: D000328:Adult; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D018562:Disease Transmission, Infectious; D005192:Family Health; D005220:Fathers; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D009035:Mothers; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid; D013290:Streptococcal Infections / Q000635:transmission*; D013295:Streptococcus mutans / Q000502:physiology*; D014421:Turkey
chemical_list: D004269:DNA, Bacterial