Eledoisin and Kassinin, but not Enterokassinin, stimulate ion transport in frog skin.
In frog skin, tachykinins stimulate the ion transport, estimated by measuring the short-circuit current (SCC) value, by interacting with NK1-like receptors. In this paper we show that Kassinin (NK2 preferring in mammals) increases the SCC, while Enterokassinin has no effect. Therefore, either 2 Pro residues or 1 Pro and 1 basic amino acid must be present in the part exceeding the C-terminal pentapeptide. Eledoisin (NK3 preferring in mammals) stimulation of SCC is reduced by CP99994 and SR48968 (NK1 and NK2 antagonists) and not affected by SB222200 (NK3 antagonist). None of the three antagonists affects Kassinin stimulation of SCC.
Lippe
Claudio
C
Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Via Amendola 165/A, 70126 Bari, Italy.
Bellantuono
Vito
V
Ardizzone
Concetta
C
Cassano
Giuseppe
G
D000595:Amino Acid Sequence; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D004600:Eledoisin / Q000378:metabolism / Q000494:pharmacology*; D017136:Ion Transport / Q000502:physiology*; D015286:Kassinin / Q000378:metabolism / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D011893:Rana esculenta / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D012879:Skin Physiological Phenomena / Q000187:drug effects; D013237:Stereoisomerism; D015320:Tachykinins / Q000378:metabolism / Q000494:pharmacology*
D001549:Benzamides; D010880:Piperidines; D011804:Quinolines; C109112:SB 222200; D015320:Tachykinins; C080755:3-(2-methoxybenzylamino)-2-phenylpiperidine; D015286:Kassinin; D004600:Eledoisin; C073839:SR 48968