Macrophages in the small intestinal muscularis externa of embryos, newborn and adult germ-free mice.
Previously, we demonstrated the presence of a constant and regularly distributed macrophage population of ramified cells in the intestinal muscle layers of smaller rodents. The function of these resident macrophages under normal conditions remains unknown. Histochemistry, immunohistochemistry and electron microscopy were applied to the muscularis externa of 15- and 17-day-old embryos, 2-day-old mice, adult germ-free and conventional mice. Since lipopolysaccharides (LPS) activates macrophages and inflammation affects gut motility, LPS-treated mice were also included in the study. Two macrophage antibodies, F4/80 and 2F8 were used to demonstrate the presence of macrophages in the muscle layers. The localization was confirmed by electron microscopy. In contrast to conventional adult mice, the muscle layers in embryos, newborn and germ-free adult mice were devoid of class II MHC antigen reactive cells. The acid phosphatase reaction and antibodies directed towards a lysosomal protein (Lamp-2) were used in order to verify other activation markers. None of these showed specific staining of the muscularis macrophages. Only LPS-treated adult mice showed iNOS-positive cells in whole mounts. We conclude that the characteristic organization and distribution of muscularis macrophages in adult mice are also present in embryos, newborn and germ-free mice and thus develop independently of foreign antigens. Further, these macrophages are truly resident and appear to have differential responses to exogene stimuli.
Mikkelsen
Hanne B
HB
Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark.
Garbarsch
Charly
C
Tranum-Jensen
Jørgen
J
Thuneberg
Lars
L
D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology / Q000502:physiology*; D015703:Antigens, CD / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D005856:Germ-Free Life; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000502:physiology* / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000633:toxicity*; D051907:Lysosomal Membrane Proteins; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009130:Muscle, Smooth / Q000502:physiology* / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II
D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D051907:Lysosomal Membrane Proteins; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse