title: Induction of callus from a metal hypertolerant fern, Athyrium yokoscense, and evaluation of its cadmium tolerance and accumulation capacity.
abstract: The callus of a metal hypertolerant fern, Athyrium yokoscense, was induced from the spores generated on a small sectioned frond in vitro. The callus grew vigorously with the periodical medium change, especially in a liquid culture. When the callus and regenerated tissues were exposed to Cd, every tissue tolerated at least 1 mM Cd for >1 month. These tissues accumulated high levels of Cd (maximum 3.3 mg g(-1) dry weight in roots) in accordance with the Cd concentration of the medium, and the Cd concentrations of all parts, except roots, were at a similar level. The data suggest that the Cd tolerance of this fern is basically independent of the plant parts and the developmental stages, although the accumulation ability is higher in roots than in the other plant parts.
authors.affiliation: Plant Molecular Biology, Laboratory of Environmental Science, Central Research Institute Electric Power Industry, 1646 Abiko, Abiko, Chiba, 270-1194, Japan. yoshiha@criepi.denken.or.jp
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yoshihara
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tsunokawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Miyano
authors.forename: Yuki
authors.initials: Y
authors.lastname: Arashima
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Hodoshima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shoji
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Goto
mesh_terms: D002104:Cadmium / Q000493:pharmacokinetics* / Q000494:pharmacology*; D029624:Ferns / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018514:Plant Structures / Q000187:drug effects / Q000502:physiology; D013170:Spores / Q000187:drug effects / Q000502:physiology
chemical_list: D002104:Cadmium