Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells.
To examine how substance P (SP) is related with dental pulp inflammation, we examined the effects of SP on expression of genes for inflammatory factors in human dental pulp cell cultures. Using reverse transcriptase-polymerase chain reaction, we found that Prevotella intermedia lipopolysaccharide (LPS) induced expression of SP and SP-receptor mRNAs, and that somatostatin inhibited the LPS-induced expression of SP mRNA. We also found that SP enhanced LPS-induced stimulation of NF-kappaB binding activity. In addition, SP induced expression of cyclooxygenase-2 and interleukin-10 receptor mRNAs. In contrast, SP inhibited expression of interferon-gamma receptor mRNA. These results suggest that SP may play a regulatory role in the immunological response of dental pulp tissue to pathogenic bacteria.
Tokuda
Masayuki
M
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima, Japan. mas-tok@dentb.hal.kagoshima-u.ac.jp
Miyamoto
Rie
R
Nagaoka
Shigetaka
S
Torii
Mitsuo
M
D019898:Autocrine Communication; D015153:Blotting, Western; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D003782:Dental Pulp / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins; D016328:NF-kappa B / Q000378:metabolism; D018713:Prevotella intermedia / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017471:Receptors, Interferon / Q000037:antagonists & inhibitors; D018123:Receptors, Interleukin / Q000096:biosynthesis; D053701:Receptors, Interleukin-10; D018040:Receptors, Neurokinin-1 / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013373:Substance P / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation; D000096743:Interferon gamma Receptor
D051546:Cyclooxygenase 2; D018836:Inflammation Mediators; D007527:Isoenzymes; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D011451:Prostaglandin-Endoperoxide Synthases; D012333:RNA, Messenger; D017471:Receptors, Interferon; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D018040:Receptors, Neurokinin-1; D013373:Substance P; D000096743:Interferon gamma Receptor; C496540:PTGS2 protein, human