Induction of callus from a metal hypertolerant fern, Athyrium yokoscense, and evaluation of its cadmium tolerance and accumulation capacity.
The callus of a metal hypertolerant fern, Athyrium yokoscense, was induced from the spores generated on a small sectioned frond in vitro. The callus grew vigorously with the periodical medium change, especially in a liquid culture. When the callus and regenerated tissues were exposed to Cd, every tissue tolerated at least 1 mM Cd for >1 month. These tissues accumulated high levels of Cd (maximum 3.3 mg g(-1) dry weight in roots) in accordance with the Cd concentration of the medium, and the Cd concentrations of all parts, except roots, were at a similar level. The data suggest that the Cd tolerance of this fern is basically independent of the plant parts and the developmental stages, although the accumulation ability is higher in roots than in the other plant parts.
Yoshihara
Toshihiro
T
Plant Molecular Biology, Laboratory of Environmental Science, Central Research Institute Electric Power Industry, 1646 Abiko, Abiko, Chiba, 270-1194, Japan. yoshiha@criepi.denken.or.jp
Tsunokawa
Kyoko
K
Miyano
Yoshiyuki
Y
Arashima
Yuki
Y
Hodoshima
Hirotaka
H
Shoji
Kazuhiro
K
Shimada
Hiroaki
H
Goto
Fumiyuki
F
D002104:Cadmium / Q000493:pharmacokinetics* / Q000494:pharmacology*; D029624:Ferns / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018514:Plant Structures / Q000187:drug effects / Q000502:physiology; D013170:Spores / Q000187:drug effects / Q000502:physiology
D002104:Cadmium