title: Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.
abstract: Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Hong
authors.forename: Todd
authors.initials: T
authors.lastname: Emrick
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D000085:Acetates / Q000737:chemistry*; D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000085:Acetates; D005026:Ethylene Glycols; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; D006046:Gold; D002918:Chymotrypsin