title: Application of high-performance liquid chromatography-tandem mass spectrometry to the identification of biologically active peptides produced by milk fermentation and simulated gastrointestinal digestion.
abstract: Identification of biologically active peptides in food matrices is a challenging task in food technology. In the present study, we propose a strategy for the rapid identification of peptides in complex food fractions and targeting of potentially bioactive peptides according to previous studies of activity-structure relationship. A large number of peptides included in the Mr 3000 permeate of a fermented product and its hydrolysate (obtained by simulated gastrointestinal digestion) could be easily identified using HPLC coupled on line to an ion trap mass spectrometer. Three of the identified sequences have previously been described as angiotensin-converting enzyme (ACE) inhibitors. The sequence of some peptides allowed us to anticipate the presence of ACE-inhibitory activity and several peptides were selected to initiate studies on antihypertensive, antioxidant and cytomodulatory activity.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernández-Ledesma
authors.forename: Lourdes
authors.initials: L
authors.lastname: Amigo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ramos
authors.forename: Isidra
authors.initials: I
authors.lastname: Recio
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D004063:Digestion; D005285:Fermentation; D006868:Hydrolysis; D007778:Lactobacillus / Q000378:metabolism; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D009862:Online Systems; D010455:Peptides / Q000032:analysis* / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides