Immunoelectron microscopic analysis of cornified cell envelopes and antigen retrieval.
In this chapter, postembedding immunoelectron microscopy methods for studies of cornified cell envelopes are provided. Human epidermal tissue samples are used as the material. The samples are cryo-fixed without chemical fixation, freeze-substituted at a low temperature, and embedded in Lowicryl K11M resin. For immunostaining, colloidal gold-conjugated secondary antibodies are used. Methods for retrieval of masked epitopes are also described.
Ishida-Yamamoto
Akemi
A
Department of Dermatology, Asahikawa Medical College, Japan.
D000180:Acrylic Resins / Q000737:chemistry; D002462:Cell Membrane / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D006801:Humans; D015603:Keratinocytes / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D011498:Protein Precursors / Q000378:metabolism*
D000180:Acrylic Resins; D008565:Membrane Proteins; D011498:Protein Precursors; C064554:loricrin; C050066:Lowicryl K11M; C032253:involucrin