[False positive and false negative reaction results based on an amplification of nucleic acids in infection diagnoses. Reasons and implications].
The paper discusses the most frequent causes of false positive and false negative results of reactions based on amplification of nucleic acids. All stages of the method are described beginning with sample collection up to the finishing of visualization of amplification product. The main aim objective of the publication is to draw doctors' attention to the necessity to interpret the result with criticism. We attempted also to demonstrate how to avoid false results.
Kamińska
Agnieszka
A
Zakład Immunopatologii Chorób Zakaźnych i Pasozytniczych Akademii Medycznej w Warszawie.
Dabrowska
Julia
J
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007239:Infections / Q000175:diagnosis* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000592:standards*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012329:RNA, Bacterial / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004279:DNA, Viral; D012329:RNA, Bacterial; D012367:RNA, Viral