Molecular cloning and characterization of a human gene involved in transcriptional regulation of hTERT.
Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase in human, has been identified as the rate-limiting factor in telomerase activity, but its detailed mechanism of transcription regulation remains unclear. In this investigation, a novel human gene telomerase transcriptional elements-interacting factor (TEIF) was isolated from HeLa cell based on hTERT promoter yeast one-hybrid assay. TEIF has a 2358-bp open reading frame encoding a predicted protein of 786 amino acids, which is functionally conserved in general eukaryotic species. The transcription of TEIF was detected in cells and expressed an 86-kDa native protein, distributing mainly in cellular nuclei. Its interaction with hTERT promoter was identified and the DNA binding potential was credited to C-terminus of TEIF. Transfection of TEIF into HeLa cells presented marked transactivation of hTERT promoter and stimulated both endogenous hTERT transcription and telomerase activities. These results suggest that TEIF protein might be a transcription regulator and take part in the activation of hTERT.
Tang
Zhiwei
Z
Department of Pathology, Health Science Center of Peking University, Beijing 100083, China.
Zhao
Yuanjun
Y
Mei
Fang
F
Yang
Shaomin
S
Li
Xuan
X
Lv
Junjie
J
Hou
Lin
L
Zhang
Bo
B
D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D040461:Gene Components; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D020218:Response Elements; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D015533:Transcriptional Activation
D033942:Adaptor Proteins, Vesicular Transport; D004268:DNA-Binding Proteins; C464929:SCYL1 protein, human; D014157:Transcription Factors; D019098:Telomerase