title: Comparison of p53 levels in lymphocytes and in blood plasma of nuclear power plant workers.
abstract: p53 levels were assessed in lymphocytes and in blood plasma of workers from two Czech nuclear power plants (NPP): 114 subjects working in Temelin and 108 subjects working in Dukovany. Ionizing radiation (IR) exposure data were available for 64 and 59 subjects working in the monitored zones from the NPP in Temelin and Dukovany, respectively. The short-term doses of IR for these subjects were 0.01 and 0.12 mSv, and the long-term doses were 0.46 and 5.68 mSv, in the Temelin and Dukovany NPP, respectively. As a control group, 46 subjects living in Ceske Budejovice, a city nearby the Temelin NPP, were analyzed. The concentration of p53 in lymphocytes was significantly higher in workers from the monitored zone in the Dukovany NPP (median value 6.4 pg/microg protein, P <0.001) than in workers from the Temelin NPP (3.2 pg/microg) as well as in the control group (3.5 pg/microg). In contrast, plasma levels of p53 were comparable in the control group (median value 116 pg/ml plasma) and workers from the monitored zone of Dukovany NPP (102 pg/ml), but lower in workers from Temelin NPP (5 pg/ml). Other factors affecting p53 levels were studied. Smoking resulted in increased p53 lymphocyte levels. The effect of polymorphisms in metabolic and DNA repair genes on p53 levels was analyzed. The correlation was found between p53 levels in lymphocytes and p53 codon 72 polymorphism in subjects working in NPPs, but not in the control group. The results of measurement p53 levels in lymphocytes suggest that this biomarker could reflect the short-term as well as long-term effects of low doses IR. Its impact on human health should be further explored.
authors.affiliation: Laboratory of Genetic Ecotoxicology, Health Institute of Central Bohemia and Institute of Experimental Medicine AS CR, Videnska 1083, 14220 Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Rössner
authors.forename: Irena
authors.initials: I
authors.lastname: Chvatalova
authors.forename: Jana
authors.initials: J
authors.lastname: Schmuczerova
authors.forename: Alena
authors.initials: A
authors.lastname: Milcova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rössner
authors.forename: Radim J
authors.initials: RJ
authors.lastname: Sram
mesh_terms: D018153:Czech Republic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D016273:Occupational Exposure; D011210:Power Plants; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
chemical_list: D016159:Tumor Suppressor Protein p53