Organization of the horizontally transferred pheBA operon and its adjacent genes in the genomes of eight indigenous Pseudomonas strains.
Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in eight different plasmids were analyzed. In all cases, miniature insertional elements or putative transposase genes were found suggesting transposase dependent pheBA integration into plasmids. In three cases, an open reading frame encoding homologue to the transcription regulator protein (CatR) of the pheBA operon was determined.
Peters
Maire
M
Institute of Molecular and Cell Biology, University of Tartu and Estonian Biocentre, 23 Riia Street, 51010 Tartu, Estonia. mpeters@ebc.ee
Tomikas
Ave
A
Nurk
Allan
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001673:Biodegradation, Environmental; D017931:DNA Primers; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D019800:Phenol / Q000493:pharmacokinetics*; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*; D015183:Restriction Mapping
D017931:DNA Primers; D019800:Phenol