title: Cooperativity between selectins and beta2-integrins define neutrophil capture and stable adhesion in shear flow.
abstract: A cooperative, sequential process of molecular recognition governs leukocyte capture, rolling, and arrest on inflamed endothelium. Flowing neutrophils are captured via heterotypic adhesive interactions mediated by endothelial E-selectin, whereas homotypic interactions between neutrophils are mediated by L-selectin. To elucidate how each selectin facilitates the transition to CD18-mediated stable adhesion, E-selectin and L-selectin were expressed at defined site density in a murine pre-B-cell line. Direct observation of two-body collisions revealed that 30% of neutrophil interactions with E-selectin transfectants formed doublets at low shear rate G = 14 s(-1) whereas a threshold shear rate 14 s(-1) < or = G < or = 10 s(-1) was necessary for L-selectin adhesion. Adhesion via L-selectin resisted rupture at high shear stress, while E-selectin tethered doublets remained intact longer once formed. Moreover, higher expression of L-selectin (1100 sites/microm2) than that of E-selectin (220 sites/microm2) was required for comparable heterotypic adhesion efficiency. With a threefold rise in active CD18 upregulated on chemotactically stimulated neutrophils, homotypic adhesion efficiency increased 10-fold compared to less than 5-fold for heterotypic adhesion to selectin transfectants. Co-expression of E-selectin and ICAM-1 boosted adhesion efficiency threefold more than either receptor alone over the range of active CD18 expression. These data are the first to quantify adhesion efficiency mediated by selectin tethering and conformational activation of beta2-integrin in neutrophils in shear flow.
authors.affiliation: Department of Biomedical Engineering, University of California, Davis, CA 95616, USA.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: McDonough
authors.forename: Fiona A
authors.initials: FA
authors.lastname: McIntosh
authors.forename: Constantina
authors.initials: C
authors.lastname: Spanos
authors.forename: Sriram
authors.initials: S
authors.lastname: Neelamegham
authors.forename: Harry L
authors.initials: HL
authors.lastname: Goldsmith
authors.forename: Scott I
authors.initials: SI
authors.lastname: Simon
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D003198:Computer Simulation; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology; D051379:Mice; D008955:Models, Cardiovascular; D018448:Models, Immunological; D018375:Neutrophil Activation / Q000502:physiology; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D019087:Selectins / Q000502:physiology*; D033081:Shear Strength; D013314:Stress, Mechanical
chemical_list: D018821:CD18 Antigens; D019087:Selectins