title: TAFI polymorphisms at amino acids 147 and 325 are not risk factors for cerebral infarction.
abstract: Thrombin-activatable fibrinolysis inhibitor (TAFI) was reported as an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). Its level in plasma appears to influence vascular disease. In addition, TAFI activity is strongly influenced by genetic polymorphism, especially at amino acids 147 and 325. We investigated whether these TAFI polymorphisms would act as a risk factor for cerebral infarction (CI) by examining 253 samples in which the diagnosis was cliniconeuropathologically confirmed. We found little that was statistically significant in terms of these polymorphisms among patients with no vascular problems or in a population-based control group. In the present study of an elderly Japanese group, our samples revealed a lower percentage of the Ile allele at Thr/Ile-325 compared with western counterparts. Although patients with severe infarcts had a lower percentage of the Ile allele (10%) at amino acid position 325 compared with the slightly and moderately affected patients and the population-based control group (15-18%), no statistical significance was found. None of our results showed any statistical correlation between TAFI polymorphisms and CI.
authors.affiliation: Choju Medical Institute, Fukushimura Hospital, Toyohashi, Aichi 441-8914, Japan. akatu@chojuken.net
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Akatsu
authors.forename: Hidehisa
authors.initials: H
authors.lastname: Yamagata
authors.forename: Yusen
authors.initials: Y
authors.lastname: Chen
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miki
authors.forename: Kouzin
authors.initials: K
authors.lastname: Kamino
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Takeda
authors.forename: William
authors.initials: W
authors.lastname: Campbell
authors.forename: Ikuko
authors.initials: I
authors.lastname: Kondo
authors.forename: Kenji
authors.initials: K
authors.lastname: Kosaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hidechika
authors.initials: H
authors.lastname: Okada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002102:Cadaver; D025901:Carboxypeptidase B2 / Q000235:genetics*; D002544:Cerebral Infarction / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors
chemical_list: D025901:Carboxypeptidase B2