title: A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype.
abstract: A novel protein MGC5306 has been identified in yeast-two-hybrid analysis by screening a HeLa cDNA library with a truncated DNA polymerasebeta (polbetaDelta) as bait. The polbetaDelta is expressed in various types of cancers. Co-immunoprecipitation-Western blot analysis confirms not only its interaction with polbetaDelta but also with wild-type polbeta. Binding to polbeta indicates potential function of MGC5306 in repair pathway. Transfection of cells with MGC5306-GFP and Western blot analysis with anti-MGC5306 antibody reveal its nuclear localization. MGC5306 is expressed in human carcinomas and tumor cell lines but not in normal tissues, suggesting MGC5306 is most likely involved in carcinogenesis. An antigrowth activity and modulations of cell cycle events are identified in cells expressing siRNAMGC5306.
authors.affiliation: Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, USA.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.forename: Nandan
authors.initials: N
authors.lastname: Bhattacharyya
authors.forename: Diane M
authors.initials: DM
authors.lastname: Chelsea
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Escobar
authors.forename: Sipra
authors.initials: S
authors.lastname: Banerjee
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002470:Cell Survival; D019951:DNA Polymerase beta / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000737:chemistry; D010641:Phenotype; D034741:RNA, Small Interfering / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D019951:DNA Polymerase beta