Mucosal delivery of anti-inflammatory IL-1Ra by sporulating recombinant bacteria.
BACKGROUND
Mucosal delivery of therapeutic protein drugs or vaccines is actively investigated, in order to improve bioavailability and avoid side effects associated with systemic administration. Orally administered bacteria, engineered to produce anti-inflammatory cytokines (IL-10, IL-1Ra), have shown localised ameliorating effects in inflammatory gastro-intestinal conditions. However, the possible systemic effects of mucosally delivered recombinant bacteria have not been investigated.


RESULTS
B. subtilis was engineered to produce the mature human IL-1 receptor antagonist (IL-1Ra). When recombinant B. subtilis was instilled in the distal colon of rats or rabbits, human IL-1Ra was found both in the intestinal lavage and in the serum of treated animals. The IL-1Ra protein in serum was intact and biologically active. IL-1-induced fever, neutrophilia, hypoglycemia and hypoferremia were inhibited in a dose-dependent fashion by intra-colon administration of IL-1Ra-producing B. subtilis. In the mouse, intra-peritoneal treatment with recombinant B. subtilis could inhibit endotoxin-induced shock and death. Instillation in the rabbit colon of another recombinant B. subtilis strain, which releases bioactive human recombinant IL-1beta upon autolysis, could induce fever and eventually death, similarly to parenteral administration of high doses of IL-1beta.


CONCLUSIONS
A novel system of controlled release of pharmacologically active proteins is described, which exploits bacterial autolysis in a non-permissive environment. Mucosal administration of recombinant B. subtilis causes the release of cytoplasmic recombinant proteins, which can then be found in serum and exert their biological activity in vivo systemically.
Porzio
Stefano
S
Inpharzam Ricerche SA, Zambon Group, Via ai Söi, CH-6807 Taverne, Switzerland. Stefano.Porzio@ZambonGroup.com <Stefano.Porzio@ZambonGroup.com>
Bossù
Paola
P
Ruggiero
Paolo
P
Boraschi
Diana
D
Tagliabue
Aldo
A
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000633:toxicity; D007413:Intestinal Mucosa / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000097:blood / Q000378:metabolism; D012795:Sialoglycoproteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D013171:Spores, Bacterial / Q000502:physiology
D000893:Anti-Inflammatory Agents; C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins