Genome-wide expression profiling of fetal membranes reveals a deficient expression of proteinase inhibitor 3 in premature rupture of membranes.
OBJECTIVE
We used a genome-wide approach to identify differentially expressed genes in patients with preterm premature rupture of membranes to improve the understanding of underlying molecular mechanisms.


METHODS
RNA was isolated from the fetal membranes of patients with preterm labor with intact membranes and preterm premature rupture of membranes and was stratified according to the presence or absence of histologic chorioamnionitis. Microarray experiments were used to identify differentially expressed genes, and real-time quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry were used in follow-up experiments.


RESULTS
Microarray experiments identified decreased expression of proteinase inhibitor 3 in the preterm premature rupture of membranes cases. Quantitative reverse transcriptase-polymerase chain reaction confirmed these results. Immunohistochemistry demonstrated decreased proteinase inhibitor 3 protein expression in preterm premature rupture of membranes.


CONCLUSIONS
A genome-wide approach identified deficient expression of proteinase inhibitor 3 in preterm premature rupture of membranes, which demonstrated the usefulness of functional genomics for the dissection of mechanisms of disease and identification of differentially regulated genes that were not suspected previously to play a role in parturition.
Tromp
Gerard
G
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA.
Kuivaniemi
Helena
H
Romero
Roberto
R
Chaiworapongsa
Tinnakorn
T
Kim
Yeon Mee
YM
Kim
Mi Ran
MR
Maymon
Eli
E
Edwin
Samuel
S
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002821:Chorioamnionitis / Q000235:genetics / Q000378:metabolism; D003430:Cross-Sectional Studies; D005321:Extraembryonic Membranes / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D036801:Parturition / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins