Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle.
Chronic respiratory infections reduce growth in pigs but protein accretion (PA) during an ongoing multifactorial respiratory infection has not been determined, and the mechanisms underlying growth inhibition are largely unknown. The objectives of this study were to determine whether viral and bacterial pneumonia in young pigs decrease PA, increase serum IL-1beta and IL-6, and increase myostatin (MSTN) mRNA in biceps femoris and triceps muscles. Mycoplasma hyopneumoniae (Mh) or medium was given intratracheally at 4 wk of age, Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) or medium was given intranasally at 6 wk of age, and pigs were killed 7 or 14 d after PRRSV inoculation for body composition analysis. PRRSV but not Mh induced a marked increase (P < 0.01) in IL-1beta, IL-6, and MSTN mRNA and a decrease (P < 0.01) in food intake, daily weight gain, PA, and lipid accretion. PRRSV also reduced (P < 0.01) myofiber area in the biceps femoris. Food intake, weight gain, PA, and weight of biceps femoris and triceps muscles were negatively correlated (r = -0.4 to -0.8, P < 0.05) with serum IL-1beta and IL-6 and with MSTN mRNA in muscle. These results suggest that the magnitude of increases in inflammatory cytokines during a respiratory infection may be predictive of decreases in PA and growth. They further suggest that during infection growth of skeletal muscle is limited in part by myostatin.
Escobar
Jeffery
J
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
Van Alstine
William G
WG
Baker
David H
DH
Johnson
Rodney W
RW
D000818:Animals; D001823:Body Composition; D004435:Eating; D015870:Gene Expression; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D050356:Lipid Metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000254:growth & development; D045705:Mycoplasma hyopneumoniae; D055435:Myostatin; D009929:Organ Size; D045729:Pneumonia of Swine, Mycoplasmal / Q000378:metabolism* / Q000503:physiopathology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000503:physiopathology; D019316:Porcine respiratory and reproductive syndrome virus; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine; D016212:Transforming Growth Factor beta / Q000235:genetics*; D015430:Weight Gain
D007375:Interleukin-1; D015850:Interleukin-6; D009124:Muscle Proteins; D055435:Myostatin; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta