Protective effect of DL-alpha-lipoic acid in cyclophosphamide induced oxidative injury in rat testis.
The aim of the present study was to evaluate the protective effect of DL-alpha-lipoic acid on the biochemical changes, tissue peroxidative damage and abnormal antioxidant levels in the rat testis during cyclophosphamide (CP)-induced injury. Adult male Wistar rats were divided into four treatment groups: (I) control, (II) 15 mg/kg CP once a week for 10 weeks by gavage, (III) 35 mg/kg lipoic acid once a week for 10 weeks by intraperitoneal injection, and (IV) CP plus lipoic acid (24 h prior to CP administration). Testicular toxicity, assessed by decreased enzymatic activities of lactate dehydrogenase and glucose-6-phosphate dehydrogenase, was reversed with lipoic acid pretreatment. CP-exposed rats (group II) showed abnormal levels of enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase) and antioxidants (reduced glutathione, ascorbate and alpha-tocopherol) along with high malondialdehyde levels. In contrast, rats pretreated with lipoic acid (group IV) showed normal lipid peroxidation and antioxidant defenses. These findings indicate a cytoprotective role of lipoic acid in this experimental model of testicular toxicity.
Selvakumar
Elangovan
E
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
Prahalathan
Chidambaram
C
Mythili
Yenjerla
Y
Varalakshmi
Palaninathan
P
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008063:Thioctic Acid / Q000008:administration & dosage / Q000494:pharmacology*
D018906:Antineoplastic Agents, Alkylating; D000975:Antioxidants; D008063:Thioctic Acid; D003520:Cyclophosphamide; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase