Beta-glucan enhancement of T cell IFNgamma response in swine.
Beta-glucan has been shown to enhance anti-tumor and anti-infection functions in animals. Pigs at 4 months of age were infected with porcine reproductive and respiratory syndrome virus (PRRSV), and peripheral blood monocytes (PBMC) were isolated for the detection of interferon gamma (IFNgamma)-producing cells. We found that soluble high molecular weight beta-glucan could increase IFNgamma-producing cell frequency in a dose-dependent manner in the enzyme-linked immunospot assay (ELISPOT) in the absence of antigenic restimulation. A concentration as low as 1.6 microg/ml gave a significant increase and a similarly high enhancement was achieved at concentrations from 3.2 to 100 microg/ml. In PRRSV-specific IFNgamma ELISPOT, soluble beta-glucan elicited increased PRRSV-specific responses at concentrations from 3.2 to 50 microg/ml, but not at 100 microg/ml, whereas insoluble beta-glucan had no effect. Soluble beta-glucan augmented the porcine cellular immune response in an antigen-independent fashion, whereas insoluble beta-glucan had no activity. This finding suggests that soluble beta-glucan may enhance innate antiviral immunity against PRRSV.
Xiao
Zhengguo
Z
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
Trincado
Carlos A
CA
Murtaugh
Michael P
MP
D000818:Animals; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D047071:beta-Glucans / Q000494:pharmacology*
D007155:Immunologic Factors; D047071:beta-Glucans; D007371:Interferon-gamma