[Study on genetic diversity of herba Cistanches by RAPD].
OBJECTIVE
To determine the genetic diversity of Cistanche species.


METHODS
Two populations of Cistanche deserticola and four populations of C. tubulosa were analyzed by random amplified polymorphic DNA (RAPD) markers.


RESULTS
A total of 76 and 87 loci were amplified using 10 random primers each other. The average percentage of polymorphic loci of C. deserticola was 47.37%. The PPL were 39.47% and 35.53% for two populations. Average Nei's gene diversity was 0.1358, Shannon' s genetic diversity was 0.2072, and Gst was 0.2546. The average PPL of C. tubulosa was 27.59%. It was 19.54% to 25.29% in different populations and Andi'er population had the highest. Average Nei's gene diversity was 0.0823, and Shannon' s genetic diversity was 0.125 8, Cst was 0.175 5.


CONCLUSIONS
The diversity of Cistanche deserticola is higher than that of C. tubulosa, but both has differentiation among populations, C. deserticola has already separated itself into two different ecotypes.
Cui
Guang-Hong
GH
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China.
Chen
Min
M
Huang
Lu-Qi
LQ
Xiao
Su-Ping
SP
Li
Da
D
D031671:Cistanche / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant