title: Deficiency of neuropilin 2 suppresses VEGF-induced retinal neovascularization.
abstract: Vascular endothelial growth factor (VEGF) plays a central role in the development of ocular neovascularization (NV) and is an excellent target for therapeutic intervention. VEGF acts through several receptors, including VEGF receptor 1, VEGF receptor 2, neuropilin-1 (Npn1), and Npn2, but the exact role of these receptors in the development of retinal NV is unknown. In this study, we investigated the expression of npn2 mRNA during new blood vessel growth in the retina and used npn2 knockout mice to assess the impact of deficiency of Npn2 on retinal NV. The level of npn2 mRNA in the retina increased during retinal vascular development, after exposure to hyperoxia, and after the onset of retinal ischemia. Immunohistochemistry showed colocalization of Npn2 with a vascular marker in retinal NV. Compared with littermate controls, mice deficient in Npn2 had significantly less ischemia-induced retinal NV and very little subretinal NV due to expression of a Vegf transgene. These data suggest that Npn2 facilitates VEGF-induced retinal NV and may constitute a useful target for therapeutic intervention in ocular diseases complicated by NV.
authors.affiliation: The Department of Ophthalmology, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287-9277, USA.
authors.forename: Jikui
authors.initials: J
authors.lastname: Shen
authors.forename: Rebecca
authors.initials: R
authors.lastname: Samul
authors.forename: Joelle
authors.initials: J
authors.lastname: Zimmer
authors.forename: Hansheng
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liang
authors.forename: Sean
authors.initials: S
authors.lastname: Hackett
authors.forename: Peter A
authors.initials: PA
authors.lastname: Campochiaro
mesh_terms: D000818:Animals; D006801:Humans; D018496:Hyperoxia / Q000150:complications; D007511:Ischemia / Q000150:complications; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D039943:Neuropilin-2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012160:Retina / Q000737:chemistry / Q000378:metabolism; D015861:Retinal Neovascularization / Q000209:etiology / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D039943:Neuropilin-2; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors