[Antimetastatic effects of genistein on salivary adenoid cystic carcinoma in vivo].
OBJECTIVE
To study the antimetastatic effects of genistein on salivary adenoid cystic carcinoma (ACC-M) in vivo.


METHODS
ACC-M cells were exposed to genistein for 3 days. The treated cells and control cells were then injected into the tail vein of 20 nude mice, which were randomized into 2 groups. The mice were sacrificed at the sixth week after implantation. The lung metastasis rates and the number of metastases were detected. Apoptosis index (AI), the expression of VEGF and MMP-9 of the lung metastases were also observed.


RESULTS
Compared to the control groups, the number of metastases was significantly reduced in genistein treated group (P < 0.05). The AI of ACC-M cells was higher in the treated group than in the control group (P < 0.05). The level of VEGF and MMP-9 expression were decreased in the treated groups (P < 0.05).


CONCLUSIONS
Genistein has a moderate inhibitory effect on metastasis of ACC. Inducing apoptosis, inhibiting the expression of VEGF and MMP-9 are the mechanisms of antimetastatic effects of genistein on ACC-M in nude mice.
Liu
Hao
H
Department of Oral and Maxillofacial Surgery, Tianjin Union Medical Center, Tianjin 300120, China.
Yu
Guang-yan
GY
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy* / Q000556:secondary; D005260:Female; D019833:Genistein / Q000008:administration & dosage*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D011897:Random Allocation; D012468:Salivary Gland Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D019833:Genistein