[Detection and sequence analysis of Sapporo-like virus in Guangzhou].
OBJECTIVE
To verify the presence of Sapporo-like virus (SLV) in children with diarrhea in Guangzhou.


METHODS
Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank.


RESULTS
Only one positive specimen (CH03354) was found out of the 169 stool specimens collected. Sequence analysis of the capsid gene of this strain showed that it belonged to SLV GI-1 cluster.


CONCLUSIONS
SLV exists in Guangzhou and different regions of China may have different genotypes of this virus.
Liu
Yi
Y
Department of Epidemiology, Southern Medical University, Guangzhou 510515, China. ly811@fimmu.com
Dai
Ying-chun
YC
Li
Jian-dong
JD
Zhan
Hui-chun
HC
Chen
Qing
Q
Yu
Shou-yi
SY
Nie
Jun
J
D001483:Base Sequence; D017250:Caliciviridae Infections / Q000821:virology*; D002675:Child, Preschool; D002681:China; D004279:DNA, Viral / Q000235:genetics; D003967:Diarrhea / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D029341:Sapovirus / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D004279:DNA, Viral