title: Stimulation of virus-specific T cell responses by dendritic cell vaccination in the chronic phase of simian AIDS models.
abstract: Virus-specific CD8+ cytotoxic T lymphocyte (CTL) responses play an important role in the control of immunodeficiency virus infections. Therapeutic immunization with antigen-pulsed dendritic cells (DC) may be a promising strategy for stimulating CTL. However, decreases in DC number and function have been suggested in the host persistently infected with the virus, and this may constitute an obstacle to DC-based immunotherapy in the chronic phase. In this study, we show that virus-specific CTL responses were augmented by therapeutic immunization with inactivated virus-pulsed autologous DC in rhesus macaques that had maintained prophylactic vaccine-based control of virus replication for more than 3 years after simian or simian-human immunodeficiency virus challenge. Our results indicate the potential of DC in the chronic phase for efficiently stimulating CTL in vivo, suggesting the feasibility of therapeutic DC immunization for replenishing virus-specific CTL responses in the chronic phase after the prophylactic vaccine-based control of primary immunodeficiency virus infection.
authors.affiliation: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Moriaki
authors.initials: M
authors.lastname: Kato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Igarashi
authors.forename: Akiko
authors.initials: A
authors.lastname: Takeda
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horie
authors.forename: Eiji
authors.initials: E
authors.lastname: Higashihara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matano
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D008253:Macaca mulatta; D008297:Male; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013997:Time Factors; D019444:Vaccines, DNA / Q000276:immunology; D019562:Viral Load
chemical_list: D018449:SAIDS Vaccines; D019444:Vaccines, DNA