title: Clustering patterns of LOD scores for asthma-related phenotypes revealed by a genome-wide screen in 295 French EGEA families.
abstract: A genome-wide scan for asthma phenotypes was conducted in the whole sample of 295 EGEA families selected through at least one asthmatic subject. In addition to asthma, seven phenotypes involved in the main asthma physiopathological pathways were considered: SPT (positive skin prick test response to at least one of 11 allergens), SPTQ score being the number of positive skin test responses to 11 allergens, Phadiatop (positive specific IgE response to a mixture of allergens), total IgE levels, eosinophils, bronchial responsiveness (BR) to methacholine challenge and %predicted FEV(1). Four regions showed evidence for linkage (P</=0.001): 6q14 for %FEV(1), 12p13 for IgE, 17q22-q24 for SPT and 21q21 for both SPTQ and %FEV(1). Nine other regions indicated smaller linkage signals (0.001<P</=0.005). While most of these regions have been reported by previous asthma and lung function screens, 6q14 appears to be a new region potentially linked to %FEV(1). To determine which of these various asthma phenotypes are more likely to share common genetic determinants, a principal component analysis was applied to the genome-wide LOD scores. This analysis revealed clustering of LODs for asthma, SPT and Phadiatop on one axis and clustering of LODs for %FEV(1), BR and SPTQ on the other, while LODs for IgE and eosinophils appeared to be independent from all other LODs. These results provide new insights into the potential sharing of genetic determinants by asthma-related phenotypes.
authors.affiliation: 1INSERM EMI0006, Evry, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bouzigon
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Dizier
authors.forename: Christine
authors.initials: C
authors.lastname: Krähenbühl
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lemainque
authors.forename: Isabella
authors.initials: I
authors.lastname: Annesi-Maesano
authors.forename: Christine
authors.initials: C
authors.lastname: Betard
authors.forename: Jean
authors.initials: J
authors.lastname: Bousquet
authors.forename: Denis
authors.initials: D
authors.lastname: Charpin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gormand
authors.forename: Michel
authors.initials: M
authors.lastname: Guilloud-Bataille
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Just
authors.forename: Nicole
authors.initials: N
authors.lastname: Le Moual
authors.forename: Jean
authors.initials: J
authors.lastname: Maccario
authors.forename: Régis
authors.initials: R
authors.lastname: Matran
authors.forename: Françoise
authors.initials: F
authors.lastname: Neukirch
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Oryszczyn
authors.forename: Evelyne
authors.initials: E
authors.lastname: Paty
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pin
authors.forename: Myriam
authors.initials: M
authors.lastname: Rosenberg-Bourgin
authors.forename: Daniel
authors.initials: D
authors.lastname: Vervloet
authors.forename: Francine
authors.initials: F
authors.lastname: Kauffmann
authors.forename: Mark
authors.initials: M
authors.lastname: Lathrop
authors.forename: Florence
authors.initials: F
authors.lastname: Demenais
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000235:genetics*; D002648:Child; D005602:France; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D008126:Lod Score; D018895:Microsatellite Repeats; D010641:Phenotype; D012907:Smoking
chemical_list: D005819:Genetic Markers