title: Rapid induction of splenic and peritoneal B-1a cells in adult mice by thymus-independent type-2 antigen.
abstract: We have produced a transgenic mouse (PV1TgL) that can only generate B lymphocytes with an Ig receptor specific for the synthetic polymer polyvinyl pyrrolidinone. Before immunization, bone marrow B cell numbers are very low, and peripheral lymphoid organs are almost devoid of B cells, confirming the role of positive selection by Ag in the development of mature B cell populations. The predominant population of B cells in the spleens of naive adult PV1TgL mice have most of the characteristics of marginal zone B cells, including anatomical location in the peripheral areas of the splenic white pulp. After immunization, a new population of B cells appears in the spleen with the characteristics of B-1 cells. Similar cells also appear somewhat later in the peritoneal cavity. Our findings suggest that immunization with a thymus-independent Ag can lead to the appearance and expansion of Ag-reactive B-1 cells in an adult mouse.
authors.affiliation: Comprehensive Center for Inflammatory Disorders, Dental Research Center, School of Dentistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Alan C
authors.initials: AC
authors.lastname: Whitmore
authors.forename: Harold R
authors.initials: HR
authors.lastname: Neely
authors.forename: Ramiro
authors.initials: R
authors.lastname: Diz
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Flood
mesh_terms: D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000235:genetics; D000955:Antigens, T-Independent / Q000008:administration & dosage* / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D003433:Crosses, Genetic; D005260:Female; D007114:Immunization / Q000379:methods; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010537:Peritoneum / Q000166:cytology* / Q000276:immunology*; D011205:Povidone / Q000008:administration & dosage / Q000031:analogs & derivatives*; D013154:Spleen / Q000166:cytology* / Q000276:immunology*
chemical_list: D000955:Antigens, T-Independent; C077842:polyvinylpolypyrrolidone; D011205:Povidone