title: Site-specific PEGylation of proteins containing unnatural amino acids.
abstract: Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.
authors.affiliation: Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
authors.forename: T Ashton
authors.initials: TA
authors.lastname: Cropp
authors.forename: Daniel
authors.initials: D
authors.lastname: Summerer
authors.forename: Mridul
authors.initials: M
authors.lastname: Mukherji
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase