Combined analysis of the essential oil of Chenopodium ambrosioides by GC, GC-MS and 13C-NMR spectroscopy: quantitative determination of ascaridole, a heat-sensitive compound.
A commercial sample of the essential oil of Chenopodium ambrosioides L. from Madagascar was analysed by GC, GC-MS and 13C-NMR. By GC analysis, the major constituents were found to be ascaridole (1) (41.8%), isoascaridole (2) (18.1%), p-cymene (16.2%), alpha-terpinene (9.7%) and limonene (3.8%). However, ascaridole undergoes a partial thermal isomerisation to 2 and hence the amount of 1 is under-estimated by GC analysis. The actual contents of 1 and 2 (55.3 and 4.6%, respectively) were obtained following combined analysis of the sample by GC and 13C-NMR. Several hydroxy- and polyhydroxy-menthanes were identified by 13C-NMR.
Cavalli
Jean-François
JF
Université de Corse, Equipe Chimie et Biomasse, UMR-CNRS 6134, Route des Sanguinaires, 20 000 Ajaccio, France.
Tomi
Félix
F
Bernardini
Antoine-François
AF
Casanova
Joseph
J
D027464:Chenopodium ambrosioides / Q000737:chemistry*; D000081005:Cyclohexane Monoterpenes; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009822:Oils, Volatile / Q000032:analysis*; D010545:Peroxides / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry
D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010545:Peroxides; D010938:Plant Oils; C043372:ascaridole