Effect of replacement of pyruvate/lactate in culture medium with glucose on preimplantation development of porcine embryos in vitro.
The effect of glucose supplementation at different times in in vitro culture on the developmental competence of in vitro produced (IVP) porcine embryos was examined. In Experiment 1, when IVP embryos were cultured in modified NCSU-37 supplemented with pyruvate and lactate (IVC-pyr/lac) for 0 h, 24 h, 48 h, 72 h, 96 h, or 118 h and subsequently in modified NCSU-37 supplemented with glucose (IVC-glu) until Day 6 (Day 0=day of in vitro fertilization), the rates of blastocyst formation were significantly higher in embryos cultured in IVC-pyr/lac for 24 or 48 h (24.4% and 23.0%, respectively) than in embryos cultured in IVC-pyr/lac for the whole culture period (14.5%). However, there were no significant differences between embryos obtained after the energy source replacement and embryos cultured in IVC-glu for the whole culture period on the rates (15.2%-24.4%, and 16.8% respectively). Replacement of pyruvate/lactate with glucose at 58 h of culture in Experiment 2 significantly enhanced the rate (31.3%) compared to those after replacement at 48 h, 53 h and 63 h of culture (20.6%, 20.8%, and 21.1%, respectively). In conclusion, replacement of pyruvate/lactate with glucose as the energy substrate was optimal at 58 h of culture for the development of porcine embryos to the blastocyst stage.
Karja
Ni Wayan Kurniani
NW
Laboratory of Animal Reproduction and Biotechnology, Department of Veterinary Sciences, Yamaguchi University, Japan.
Medvedev
Sergey
S
Onishi
Akira
A
Fuchimoto
Dai-Ichiro
D
Iwamoto
Masaki
M
Otoi
Takeshige
T
Nagai
Takashi
T
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D005947:Glucose / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000494:pharmacology*; D034421:Sus scrofa
D003470:Culture Media; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose