Synthesis of long Poly(dG).Poly(dC) DNA using enzymatic reaction.
Non-defect Poly(dG).Poly(dC) of 500 bp (170 nm) has been synthesized by using enzymatic reactions and was characterized by its UV spectrum, showing that conjugated pi-electrons between base pairs are spread over the DNA molecule suggesting the absence of structural defects.
Tanaka
Shin-ichi
S
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0074, Japan.
Taniguchi
Masateru
M
Uchiyama
Susumu
S
Fukui
Kiichi
K
Kawai
Tomoji
T
D004247:DNA / Q000138:chemical synthesis*; D011088:DNA Ligases / Q000737:chemistry*; D004583:Electrons; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet
D011089:Polydeoxyribonucleotides; C016681:poly(dG).poly(dC); D004247:DNA; D011088:DNA Ligases