Analysis of differential gene expression in the cochlea and kidney of mouse by cDNA microarrays.
Microarray hybridization analysis of gene expression in the cochlea and kidney suggest a relationship between these tissues at the genomic level, indicating the common gene expression, likely serving a common function in both the organs primarily maintaining ion transport, and implied previously from morphological, pharmaco-kinetic and teratogenic studies. The cDNAs of more than 100 genes listed on the hereditary hearing loss homepage were amplified as targets by RT-PCR and were hybridized with probes prepared from total RNA of the cochlea and the kidney. Thirteen of the genes analyzed showed altered fluorescence ratios of more than two logs. Of these, the expressions of 11 genes were over expressed and two were under expressed in the cochlea than in the kidney. Our data is the first report to corroborate the genomic similarities between these two important organs and may help to explain the somewhat similar response of these organs to certain therapeutic drugs.
Liu
Xin
X
Department of Otolaryngology, National University Hospital, Main Building, Level 3, Singapore.
Mohamed
Jamal A
JA
Ruan
Runsheng
R
D000818:Animals; D003051:Cochlea / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016678:Genome; D007668:Kidney / Q000502:physiology*; D008297:Male; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D023421:Models, Animal; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D009418:S100 Proteins; D028021:Sodium-Potassium-Chloride Symporters; D019204:GTP-Binding Proteins; D000254:Sodium-Potassium-Exchanging ATPase