Ex vivo responsiveness of head and neck squamous cell carcinoma to glufosfamide, a novel alkylating agent.
BACKGROUND
Glufosfamide is a novel alkylating agent in which the active metabolite of isophosphoramide mustard is glycosidically linked to beta-D-glucose. Targeting the elevated glucose uptake of tumor cells expressing the SAAT1 glucose transporter, glufosfamide represents an attractive new drug for cancer chemotherapy. The present study investigates the ex vivo responsiveness of Head and Neck Squamous Cell Carcinoma (HNSCC) specimens to glufosfamide.


METHODS
Twenty-one unselected HNSCC specimens were investigated using a novel ex vivo colony formation assay to determine the epithelial drug response. The individual responsiveness to glufosfamide and to cis-platinum was determined.


RESULTS
Five out of 21 evaluable HNSCC specimens were sensitive to glufosfamide. There was a tendency for glufosfamide sensitivity in platinum-resistant specimens and vice versa.


CONCLUSIONS
The effectiveness of glufosfamide observed in the present ex vivo study suggests at least an equipotentiality of glufosfamide in comparison to cis-platinum. The potential clinical usefulness of glufosfamide in HNSCC warrants further evaluation.
Dollner
Ralph
R
Department of Otorhinolaryngology, Head and Neck Surgery, University of Leipzig, Leipzig, Gernany. Ralph.Dollner@medizin.uni-leipzig.de
Dietz
Andreas
A
Kopun
Marijana
M
Helbig
Matthias
M
Wallner
Frank
F
Granzow
Christof
C
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D002945:Cisplatin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005947:Glucose / Q000031:analogs & derivatives; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells / Q000187:drug effects; D010752:Phosphoramide Mustards / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D018906:Antineoplastic Agents, Alkylating; D010752:Phosphoramide Mustards; C092028:beta-D-glucosylisophosphoramide mustard; D005947:Glucose; D002945:Cisplatin; D007069:Ifosfamide