Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.
A protocol for detection of mutations in the TP53 gene using temporal temperature gradient gel electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques denaturing gradient gel electrophoresis (DGGE) and constant denaturant gel electrophoresis (CDGE) and eliminates some of the problems. The result is a rapid and sensitive screening technique that is robust and easily set up in smaller laboratory environments.
Sørlie
Therese
T
Department of Genetics, The Norwegian Radium Hospital, Oslo, Norway.
Johnsen
Hilde
H
Vu
Phuong
P
Lind
Guro Elisabeth
GE
Lothe
Ragnhild
R
Børresen-Dale
Anne-Lise
AL
D004247:DNA / Q000032:analysis / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics; D009691:Nucleic Acid Denaturation; D013696:Temperature
D004247:DNA