title: Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity.
abstract: Induction of immunity and peripheral tolerance requires contacts between antigen-bearing dendritic cells (DCs) and cognate T cells. Using real-time two-photon microscopy, we have analyzed the dynamics of CD8(+) T cells in lymph nodes during the induction of antigen-specific immunity or tolerance. At 15-20 h after the induction of immunity, T cells stopped moving and established prolonged interactions with DCs. In tolerogenic conditions, despite effective initial T cell activation and proliferation, naive T cells remained motile and established serial brief contacts with multiple DCs. Thus, stable DC-T cell interactions occur during the induction of priming, whereas brief contacts may contribute to the induction of T cell tolerance.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U365, Immunité et Cancer, Institut Curie, 26 rue d'Ulm, F-75248 Paris Cedex 05, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Hugues
authors.forename: Luc
authors.initials: L
authors.lastname: Fetler
authors.forename: Laura
authors.initials: L
authors.lastname: Bonifaz
authors.forename: Julie
authors.initials: J
authors.lastname: Helft
authors.forename: François
authors.initials: F
authors.lastname: Amblard
authors.forename: Sebastian
authors.initials: S
authors.lastname: Amigorena
mesh_terms: D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology*; D013997:Time Factors