Preparative linear ion trap mass spectrometer for separation and collection of purified proteins and peptides in arrays using ion soft landing.
A preparative mass spectrometer for microarray fabrication is reported. The instrument includes an atmospheric pressure ionization source, a linear ion trap mass analyzer, an ion collection surface positioning system, and a surface loading chamber with independent vacuum pumping. It was designed for the production of protein arrays using the ion soft-landing technique to collect ions on a surface after separation by mass/charge ratio. Small microarrays have been prepared by isolating and soft landing individual protein or peptide ions after electrospray ionization of mixtures. The composition and purity of the separated materials has been confirmed using independent external mass spectrometric analysis of rinse solutions of the collected spots, either by the new method of electrosonic spray ionization MS or by nanospray ionization MS. The ability to retain bioactivity in the mass-selected and collected biomolecules has been demonstrated in particular cases. The reported instrument has also been characterized as an analytical mass spectrometer.
Blake
Thomas A
TA
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Ouyang
Zheng
Z
Wiseman
Justin M
JM
Takáts
Zoltán
Z
Guymon
Andrew J
AJ
Kothari
Sameer
S
Cooks
R Graham
RG
D000595:Amino Acid Sequence; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D036103:Nanotechnology; D055095:Optics and Photonics; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
D007477:Ions; D010455:Peptides; D011506:Proteins