Antigen-presenting cells are targets of regulatory T cells similar to those that mediate anterior chamber-associated immune deviation.
OBJECTIVE
To identify the means by which in-vitro-generated regulatory T cells, similar to those in anterior chamber-associated immune deviation (ACAID), suppress antigen-specific T-cell responses.


METHODS
T regulators (T regs), generated by stimulating ovalbumin (OVA)-specific Tcr transgenic DO11.10 T cells with OVA-pulsed, TGF-beta2-treated peritoneal exudates cells (PEC), or their supernatants were added to OVA-pulsed PEC that were used to activate DO11.10 T cells in vitro or to suppress OVA-specific delayed hypersensitivity (DH) induction in vivo.


RESULTS
OVA-pulsed PECs exposed in vitro to TGF-beta-producing T regs or their supernatants failed to activate DO11.10 T cells in vitro, and suppressed DH in mice immunized with OVA plus adjuvant.


CONCLUSIONS
T cells exposed to TGF-beta2-pretreated, antigen-pulsed PECs secrete soluble factors, including active TGF-beta that regulate OVA-specific responses by forcing antigen-presenting cells to promote deviant T-cell activation in vitro and in vivo.
Yokoi
Hidetoshi
H
The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Streilein
J Wayne
JW
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002478:Cells, Cultured; D005122:Exudates and Transudates / Q000276:immunology / Q000378:metabolism; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007154:Immune System Diseases / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053781:Transforming Growth Factor beta2
D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D010047:Ovalbumin