title: Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K.
abstract: The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.
authors.affiliation: Department of Biochemistry and Microbiology, Enzyme and Microbial Technology Research, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. rnzaliha@fsas.upm.edu.my
authors.forename: Raja Noor Zaliha Abd
authors.initials: RN
authors.lastname: Rahman
authors.forename: Lee Poh
authors.initials: LP
authors.lastname: Geok
authors.forename: Mahiran
authors.initials: M
authors.lastname: Basri
authors.forename: Abu Bakar
authors.initials: AB
authors.lastname: Salleh
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D004361:Drug Tolerance; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D009038:Motion; D009930:Organic Chemicals / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology*; D012997:Solvents / Q000737:chemistry*; D013045:Species Specificity; D013696:Temperature
chemical_list: D009930:Organic Chemicals; D012997:Solvents; D010447:Peptide Hydrolases