title: [Immunoexpression of c-erbB-2 in intraductal proliferative lesions of the female breast].
abstract: OBJECTIVE
  Genetic modifications are related to genesis and development of cancer. Neoplasias in various organs express the c-erbB-2 oncogene. In intraductal proliferations of the breast it has been assessed as a risk factor for subsequent development of carcinoma. The c-erbB-2 immunoexpression in intraductal epithelial proliferations and the relationship with histopathological characteristics of ductal carcinoma in situ (DCIS) were evaluated.
  
  
  METHODS
  File material from 88 women, which were tissue samples formalin-fixed, paraffin-embedded blocks, was used. Of these 51 presented with DCIS and 37 with ductal hyperplasia without atypia. Ages of the women ranged from 35 to 76 years. All cases were reviewed and nuclear grade, presence of necrosis, preponderance of histological subtype and its extension were verified. Specimens were obtained for the c-erB-2 immunohistochemical study of 84 of the women in question.
  
  
  RESULTS
  No expression of the oncogene was verified in the hyperplasias without atypias and in tissues adjacent to all tissue samples. Expression of c-erbB-2 was verified in 9 (19.1%) of the DCIS (p = 0.0001). Immunoexpression was not related to the extension of the lesions. The c-erbB-2 immunoexpression in DCIS was correlated to the histological subtype (p = 0.019), necrosis (p = 0.0066), nuclear grade (p = 0.0084) and Van Nuys Classification (p = 0.039).
  
  
  CONCLUSIONS
  Expression of c-erbB-2 was significant in proliferative lesions with risk (DCIS) and was correlated to histopathological characteristics: high nuclear grade, presence of necrosis and comedy subtype. There was no expression in the hyperplasias without atypias and adjacent tissues.
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Botucatu, UNESP, Botucatu, SP.
authors.forename: Agliberto Barbosa de
authors.initials: AB
authors.lastname: Oliveira
authors.forename: Laurival Antônio
authors.initials: LA
authors.lastname: De Luca
authors.forename: Grigna Teixeira
authors.initials: GT
authors.lastname: Carvalho
authors.forename: Victor Eduardo Arua
authors.initials: VE
authors.lastname: Arias
authors.forename: Lídia Raquel de
authors.initials: LR
authors.lastname: Carvalho
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Assunção
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002278:Carcinoma in Situ / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018734:Genes, erbB-2; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D012189:Retrospective Studies
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases