Three new DRB alleles routinely identified by sequence-based typing: DRB1*010103, DRB1*0326 and DRB3*0219.
We describe here two additional DRB1 alleles found in two Caucasoid recipient candidates for organ transplant and a new DRB3 allele found in a Caucasoid unrelated bone marrow donor from the German file. HLA-DRB generic and allele typing were performed using commercial kits, subsequently exon 2 was sequenced. We found a DRB1*010101 with a silent mutation at codon 68 and a DRB1*0306 with a mutation at codon 38 (T-C) which causes an amino acid substitution from Val to Ala. DRB3*0219 differs from DRB3*020201 by two-point mutations at codons 60 and 74 (A/C and A/G, respectively). These mutations at positions 266 and 308 were responsible for two amino acid substitutions (Tyr to Ser and Gln to Arg).
Dubois
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V
Histocompatibility Laboratory, EFS Rhône Alpes, Lyon, France.
Favre-Victoire
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I
Gebuhrer
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L
D001483:Base Sequence; D015234:HLA-A Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D059826:HLA-DRB3 Chains; D006801:Humans; D008969:Molecular Sequence Data
D015234:HLA-A Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C487961:HLA-DRB1*01:01 antigen; C497989:HLA-DRB1*01:01:03 antigen; C497988:HLA-DRB1*03:26 antigen; D059826:HLA-DRB3 Chains; C497987:HLA-DRB3*02:19 antigen