Dynamic interactions of a transcription factor with DNA are accelerated by a chromatin remodeller.
Most components in the nucleus are in a state of dynamic equilibrium maintained by the rapid mobility of nuclear proteins within and between compartments. Mobility is believed to reflect transient binding, but the identity of the binding sites and the function of the transient interactions are a matter of debate. Furthermore, we know little about how these processes may be regulated. Here, we investigate the nature and regulation of transcription factor binding and mobility in the nucleus of yeast cells. Using the Ace1p transcriptional activator, we show that nonspecific DNA binding interactions seem to have a role in retarding Ace1p nuclear mobility. Surprisingly, we find that this binding is a regulated process using a chromatin remodeller to speed up Ace1p interactions at nonspecific DNA sites. Our results suggest that transcription factor mobility represents a diffusion-driven, rapid sampling of nonspecific DNA sites, and that chromatin remodellers accelerate this genomic search process.
Karpova
Tatiana S
TS
CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI, NIH, 41 Library Drive, Bethesda, Maryland 20892, USA.
Chen
Teresa Y
TY
Sprague
Brian L
BL
McNally
James G
JG
D001665:Binding Sites; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006720:Homozygote; D007700:Kinetics; D009068:Movement; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry*; D015533:Transcriptional Activation
C060256:CUP2 protein, S cerevisiae; D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D004247:DNA