title: Hepatobiliary changes, antibody response, and alteration of liver enzymes in hamsters re-infected with Opisthorchis viverrini.
abstract: We investigated pathological changes, antibody response, and liver enzymes in hamsters re-infected with Opisthorchis viverrini. Group 1 received a single dose of 50 metacercariae; Groups 2 and 3 were first dosed with of 30 metacercariae and re-infected with 20 more once or twice at three month intervals. Inflammation and liver cell necrosis were observed on 3D (day 3) for Group 3 and 7D for Group 2 in comparison with 21D for Group 1. Pathological changes included peri-ductal fibrosis, bile duct dilation, and small bile duct formation. Increased O. viverrini-specific IgG levels ranked in the order Group 3>Group 2>Group 1. Liver enzyme activity was related to inflammatory cell infiltration. Re-infection induced faster inflammation and more severe pathological changes in association with parasite-specific antibody during chronic inflammation. This study emphasizes that there is an important relationship between the gradual decreases of inflammation with a concomitant increase in fibrosis after re-infection.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Pinlaor
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Puangrat
authors.initials: P
authors.lastname: Yongvanit
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D001219:Aspartate Aminotransferases / Q000378:metabolism; D006224:Cricetinae; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008099:Liver / Q000201:enzymology* / Q000469:parasitology / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D009889:Opisthorchiasis / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D009891:Opisthorchis / Q000276:immunology / Q000472:pathogenicity*; D010270:Parasite Egg Count; D012008:Recurrence; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase