Genomic in situ hybridization in plants with small genomes is feasible and elucidates the chromosomal parentage in interspecific Arabidopsis hybrids.
Genomic in situ hybridization (GISH) is a useful tool to analyse natural polyploids, hybrid plants, and their backcross progenies as to their origin, genomic composition, and intergenomic rearrangements. However, in angiosperms with very small genomes (<0.6 pg/1 C), often only heterochromatic regions were found to be labeled. We have modified the GISH technique to label entire mitotic and meiotic chromosomes of Arabidopsis thaliana (2n = 10) and closely related species with very small genomes by using high concentrations of DNA (7.5-15 microg per probe per slide) or 5 microg of probe and long hybridization times (>60 h). According to our GISH data, Cardaminopsis carpatica (2n = 16) is most likely the diploid ancestor of the autotetraploid Arabidopsis arenosa (2n = 32). Furthermore, within the allotetraploid species Arabidopsis suecica (2n = 26), it was possible to elucidate the origin of chromosomes contributed by the parental species A. thaliana and A. arenosa for a specimen with 2n = 26 or a deviating chromosome number.
Ali
Hoda B M
HB
Institute of Plant Genetics and Crop Plant Research (IPK), D-06466 Gatersleben, Germany.
Lysak
Martin A
MA
Schubert
Ingo
I
D017360:Arabidopsis / Q000235:genetics*; D002678:Chimera / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D018745:Genome, Plant; D008540:Meiosis / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D011123:Polyploidy