title: Inhibitory activity of Chrysanthemi sibirici herba extract on RBL-2H3 mast cells and compound 48/80-induced anaphylaxis.
abstract: The effects of extracts from various oriental medicinal herbs on mast cell-mediated allergic reaction were investigated. Among them, Chrysanthemi sibirici herba ethanol extract exerted the potent inhibitory activity on antigen-induced degranulation in RBL-2H3 mast cells. Chrysanthemi sibirici herba dose-dependently inhibited DNP-BSA or compound 48/80-induced degranulation in RBL-2H3 mast cells, with IC(50) values of approximately 49 microg/ml and 76 microg/ml, respectively. This extract strongly suppressed compound 48/80-induced systemic anaphylaxis by 48.7% at a dose of 300 mg/kg in mice. Chrysanthemi sibirici herba also inhibited the expression of TNF-alpha and the activation of the MAP kinase, ERK1/2, which is critical for the production of inflammatory cytokines in mast cells, as indicated by the suppression of activating phosphorylation of ERK1/2. These results lead us to conclude that Chrysanthemi sibirici herba may be used clinically to treat various allergic diseases.
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Seo
authors.forename: Na Young
authors.initials: NY
authors.lastname: Ko
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Chang
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Taekyu
authors.initials: T
authors.lastname: Park
authors.forename: Hoi Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Jeung Whan
authors.initials: JW
authors.lastname: Han
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology; D031202:Chrysanthemum; D004305:Dose-Response Relationship, Drug; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000302:isolation & purification / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D010936:Plant Extracts; D028321:Plant Preparations; D003189:p-Methoxy-N-methylphenethylamine