title: Spasmogenic effect of a Solanummelongena leaf extract on guinea pig tracheal chains and its possible mechanism(s).
abstract: The methanol extract of fresh leaves of Solanum melongena L. (Solanaceae) was evaluated for its capacity to alter the tone of isolated, pre-contracted guinea pig tracheal chains, as well as for its possible mechanism(s) of action. Using serial dilutions between 0.0025 and 2.5 mg/mL, the extract was found to cause a dose-dependent increase in the force of muscle contraction. The EC(50) value was 0.46 +/- 0.01 mg/mL. The concomitant use of acetylcholine 10(-5) M did not significantly affect the force of contraction induced by the extract. Histamine 10(-5) M added at about 40% to, and salbutamol 10(-6) M antagonized by about 30% its constrictive effect. Chlorpheniramine 10(-6) M, propanolol 10(-5) M, and nifedipine 10(-6) M did not significantly influence the extract-induced force of contraction, but atropine 3 x 10(-7) M reduced it by approximately 60%. These data suggest that the Solanum melongena extract exerted a bronchospasmogenic rather than a bronchospasmolytic effect, probably through muscarinic receptor stimulation.
authors.affiliation: Department of Pharmacology, Faculty of Medical Sciences, Anton de Kom University, Kernkampweg 5-7, Paramaribo, Suriname. dennisra@sr.net
authors.forename: D R A
authors.initials: DR
authors.lastname: Mans
authors.forename: J
authors.initials: J
authors.lastname: Toelsie
authors.forename: S
authors.initials: S
authors.lastname: Mohan
authors.forename: S
authors.initials: S
authors.lastname: Jurgens
authors.forename: M
authors.initials: M
authors.lastname: Muhringen
authors.forename: S
authors.initials: S
authors.lastname: Illes
authors.forename: R
authors.initials: R
authors.lastname: Macnack
authors.forename: R
authors.initials: R
authors.lastname: Bipat
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D019657:Solanaceae; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
chemical_list: D010936:Plant Extracts; D000109:Acetylcholine