Immunodetection of UCP1 in rat thymocytes.
Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This implies that rat thymocytes contain uncoupling protein 1.
Carroll
A M
AM
Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland.
Haines
L R
LR
Pearson
T W
TW
Brennan
C
C
Breen
E P
EP
Porter
R K
RK
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007473:Ion Channels; D008565:Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification; D024101:Mitochondrial Proteins; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000378:metabolism*; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1