A homodecoupled diffusion experiment for the analysis of complex mixtures by NMR.
Diffusion ordered spectroscopy (DOSY) relies on differences in translation diffusion as a means to separate components in a solution mixture. However, the analysis of spectra of mixtures can be problematic because spectral overlap. It is the aim of this article to propose a pulse sequence and processing method that leads to a complete 2D homodecoupled-DOSY experiment. This experiment offers several advantages that could extend the range of applications to more complex mixtures by achieving important improvements in both signal dispersion and sensitivity.
Cobas
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JC
Laboratorio Integral de Dinámica e Estructura de Biomoléculas José R. Carracido, Unidade de Resonancia Magnética, Edificio Cactus, RIAIDT, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. carlos@mestrec.com
Martín-Pastor
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D001552:Benzazepines / Q000737:chemistry; D045424:Complex Mixtures / Q000737:chemistry*; D004058:Diffusion; D004867:Equipment Design; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods*; D013395:Sucrose / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
D001552:Benzazepines; D045424:Complex Mixtures; D047392:beta-Cyclodextrins; D013395:Sucrose; C031215:betadex