Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection.
The design and construction of a long (75-mer) oligonucleotide-based DNA microarray for herpes simplex virus type 2 transcripts is described. This array is utilized to generate an analysis of HSV-2 transcript abundance as a function of conditions of infection of human cells, and global patterns of HSV-2 transcript abundance are compared with those for HSV-1. General similarities in patterns along with notable differences in specific details are noted. These results reveal a marked conservation in the program of gene activity between phenotypically diverged strains.
Aguilar
J S
JS
Department of Molecular Biology and Biochemistry, and Center for Virus Research, University of California-Irvine, Irvine, CA, USA.
Ghazal
Peter
P
Wagner
Edward K
EK
D002874:Chromosome Mapping; D006558:Herpes Genitalis / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000145:classification / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger