title: Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin.
abstract: The antiterminator Q gene of bacteriophage 933W (Q933) was identified upstream of the stx2 gene in 90% of human disease-origin Escherichia coli O157:H7 isolates and in 44.5% of bovine isolates. Shiga toxin production was higher in Q933-positive isolates than Q933-negative isolates. This genetic marker may provide a useful molecular tool for epidemiologic studies.
authors.affiliation: Ohio State University, Wooster, Ohio 44691, USA. lejeune.3@osu.edu
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Lejeune
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Abedon
authors.forename: Kaori
authors.initials: K
authors.lastname: Takemura
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Christie
authors.forename: Srinand
authors.initials: S
authors.lastname: Sreevatsan
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003090:Coliphages / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000821:virology; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D022641:Shiga Toxin 2 / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D005819:Genetic Markers; C066119:Q protein, Bacteriophage lambda; D022641:Shiga Toxin 2; D014764:Viral Proteins