High-performance liquid chromatography-coordination ion spray-mass spectrometry (HPLC-CIS/MS): a new tool for the analysis of non-polar compound classes in plant extracts using the example of Piper methysticum Forst.
An efficient method to characterise complex plant extracts is described using the example of Piper methysticum Forst. (kava; Piperaceae). The method is based on the on-line coupling of high-performance liquid chromatography to a new detection technique: coordination ion spray-mass spectrometry (CIS/MS). CIS/MS is a universal, novel ionisation technique improving selectivity as well as sensitivity. Charged complexes were formed through addition of central complexing ions such as sodium, silver and cobalt. The advantages of CIS/MS detection compared with the electrospray ionisation detection are discussed. The experimental set-up and the application of this simple and robust technique is described to show the its various fields of application in the analysis of plant extracts.
Gaub
M
M
Institute of Pharmacy, Department of Pharmaceutical Analysis, University of Tübingen, Auf der Morgenstelle 8, D-72076 Tübingen, Germany.
von Brocke
A
A
Roos
G
G
Kovar
K A
KA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020901:Kava / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D011753:Pyrones / Q000032:analysis*
D010936:Plant Extracts; D011753:Pyrones