title: Joint tests for quantitative trait loci in experimental crosses.
abstract: Selective genotyping is common because it can increase the expected correlation between QTL genotype and phenotype and thus increase the statistical power of linkage tests (i.e., regression-based tests). Linkage can also be tested by assessing whether the marginal genotypic distribution conforms to its expectation, a marginal-based test. We developed a class of joint tests that, by constraining intercepts in regression-based analyses, capitalize on the information available in both regression-based and marginal-based tests. We simulated data corresponding to the null hypothesis of no QTL effect and the alternative of some QTL effect at the locus for a backcross and an F2 intercross between inbred strains. Regression-based and marginal-based tests were compared to corresponding joint tests. We studied the effects of random sampling, selective sampling from a single tail of the phenotypic distribution, and selective sampling from both tails of the phenotypic distribution. Joint tests were nearly as powerful as all competing alternatives for random sampling and two-tailed selection under both backcross and F2 intercross situations. Joint tests were generally more powerful for one-tailed selection under both backcross and F2 intercross situations. However, joint tests cannot be recommended for one-tailed selective genotyping if segregation distortion is suspected.
authors.affiliation: Department of Biostatistics, Section on Statistical Genetics, University of Alabama at Birmingham, Birmingham, AL, USA. MBeasley@UAB.edu
authors.forename: T Mark
authors.initials: TM
authors.lastname: Beasley
authors.forename: Dongyan
authors.initials: D
authors.lastname: Yang
authors.forename: Nengjun
authors.initials: N
authors.lastname: Yi
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Bullard
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Travis
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Amos
authors.forename: Shizhong
authors.initials: S
authors.lastname: Xu
authors.forename: David B
authors.initials: DB
authors.lastname: Allison
mesh_terms: D003198:Computer Simulation; D003433:Crosses, Genetic; D018562:Disease Transmission, Infectious; D005828:Genetics, Population; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics*; D008433:Mathematics; D015233:Models, Statistical; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D012641:Selection, Genetic