title: Human endothelial cell attachment and proliferation on a novel vascular graft prototype.
abstract: A new vascular prosthesis prototype was assessed for its ability to support an endothelial cell layer in vitro. A coiled tubular structure, constructed from polymer-coated metallic wires, with an internal diameter of 690 microm, was used. Addition of heparin to the surface coating of the coil strongly enhanced the blood compatibility of the device. A series of coils with five different coatings, increasing in hydrophilicity, was studied. Heparin was added to one series, another series did not contain this anticoagulant drug. Upon contact with blood, a vascular prosthesis will instantaneously adsorb plasma proteins on its surface, and these proteins will influence the behavior of cells binding to the device. When coils were treated with human plasma proteins, mimicking the in vivo situation, human microvascular endothelial cells grew well on all coils studied, irrespective of the hydrophilicity of the underlying coating or the addition of heparin. For control coils, only endothelial cell growth on the most hydrophobic surfaces, and a moderate enhancing effect for heparin, were observed. This novel vascular graft prototype seems well suited for the support of an endothelial cell layer, especially when plasma proteins are adsorbed to its surface, and shows promise for in vivo testing.
authors.affiliation: Centre for Biomaterials Research, University of Maastricht, PO Box 616, 6200 MD, Maastricht, The Netherlands. menno.knetsch@bioch.unimaas.nl
authors.forename: Menno L W
authors.initials: ML
authors.lastname: Knetsch
authors.forename: Yvette B J
authors.initials: YB
authors.lastname: Aldenhoff
authors.forename: Martijn
authors.initials: M
authors.lastname: Schraven
authors.forename: Leo H
authors.initials: LH
authors.lastname: Koole
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D042783:Endothelial Cells / Q000502:physiology*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000737:chemistry; D013242:Sterilization
chemical_list: D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D006493:Heparin