title: NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.
abstract: Transcriptional activity of the TATA-binding protein (TBP) is controlled by a variety of proteins. The BTAF1 protein (formerly known as TAF(II)170/TAF-172 and the human ortholog of Saccharomyces cerevisiae Mot1p) and the NC2 complex composed of NC2alpha (DRAP1) and NC2beta (Dr1) are able to bind to TBP directly and regulate RNA polymerase II transcription both positively and negatively. Here, we present evidence that the NC2alpha subunit interacts with BTAF1. In contrast, the NC2beta subunit is not able to associate with BTAF1 and seems to interfere with the BTAF1-TBP interaction. Addition of NC2alpha or the NC2 complex can stimulate the ability of BTAF1 to interact with TBP. This function is dependent on the presence of ATP in cell extracts but does not involve the ATPase activity of BTAF1 nor phosphorylation of NC2alpha. Together, our results constitute the first evidence of the physical cooperation between BTAF1 and NC2alpha in TBP regulation and provide a framework to understand transcription functions of NC2alpha and NC2beta in vivo.
authors.affiliation: Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands.
authors.forename: Marcin P
authors.initials: MP
authors.lastname: Klejman
authors.forename: Lloyd A
authors.initials: LA
authors.lastname: Pereira
authors.forename: Hester J T
authors.initials: HJ
authors.lastname: van Zeeburg
authors.forename: Siv
authors.initials: S
authors.lastname: Gilfillan
authors.forename: Michael
authors.initials: M
authors.lastname: Meisterernst
authors.forename: H T Marc
authors.initials: HT
authors.lastname: Timmers
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D014443:Tyrosine / Q000737:chemistry
chemical_list: C109035:BTAF1 protein, human; C099112:DRAP1 protein, human; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014443:Tyrosine; D000255:Adenosine Triphosphate; D004247:DNA