[Correlation between local hormones and CD36 transcription level in women with polycystic ovary].
OBJECTIVE
To detect CD(36) expressions in polycystic ovary (PCO), and to explore its correlation with local androgen and insulin at transcription level.


METHODS
From August 2002 to February 2003, 12 patients with asymmetric PCO, 15 primary or secondary infertile patients without endocrine disorders and 8 polycystic ovary syndrome (PCOS) with bilateral PCO were recruited. Extraction of follicular fluid and detection of testosterone (T), dehydroepiandrosterone sulfate (DHEAS), insulin (INS) and androstenedione (A(2)) were performed. Relative CD(36) mRNA expression level of human ovarian inner thecal cells was analyzed by auto image analysis system (IAS) after RT-PCR.


RESULTS
The level of CD(36) mRNA expression in thecal cells was 0.24 +/- 0.07 in polycystic ovary of PCO group and 0.21 +/- 0.05 in bilateral ovaries of PCOS group, respectively, which were significantly lower than 0.83 +/- 0.13 in normal ovaries (P < 0.01). T and INS levels of follicle fluid in PCO were significantly higher than that in normal ovaries (P < 0.01). T and INS levels of follicle fluid were negatively related to CD(36) mRNA expression of follicular theca interna (r = -0.6810, r = -0.6708, P < 0.01).


CONCLUSIONS
Decrease of scavenger receptor gene CD(36) mRNA may play a role in the pathogenesis of PCO by increasing the level of T and INS in follicular fluid.
Yao
Liang-qing
LQ
Department Obstetrics and Gynecology, Affiliated Second Hospital, Zhongshan University, Guangzhou 510120, China.
Kuang
Jian-quan
JQ
Yang
Dong-zi
DZ
Li
Lin
L
He
Ying-ming
YM
Li
Guang-yi
GY
Chen
Lu-shi
LS
D000328:Adult; D000735:Androstenedione / Q000378:metabolism; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D013739:Testosterone / Q000378:metabolism*; D013799:Theca Cells / Q000378:metabolism
D018955:CD36 Antigens; D007328:Insulin; D012333:RNA, Messenger; D013739:Testosterone; D000735:Androstenedione