[The confirmation of putative natural hybrid species Meconopsis x cookei G. Taylor (Papaveraceae) based on nuclear ribosomal DNA ITS region sequence].
The Nuclear Ribosomal DNA internal transcribed spacers (ITS) region sequences from a putative natural hybrid species Meconopsis x cookei and its possible parents M. punicea and M. quintuplinervia were obtained by using direct sequencing method. The sequence length of ITS region (including ITS1, 5.8S and ITS2) is 667 bp for M. punicea, 668 bp for M. x cookei, and 668 bp for M. quintuplinervia. The sequences were aligned by the software Clustal X, and the bases per locus were compared by using software with manual method. The aligned sequence length is 688 bp, of which ITS1 is 254 bp, 5.8S is 162 bp, and ITS2 is 252 bp. 16 variable loci were detected from the aligned sequence, approximately 2.40% to the whole sequence length, of which ITS1 has nine variable loci (56.25%), ITS2 has six variable loci (37.50%), and 5.8S has one variable locus (6.25%). The results show that M. x cookei have two kinds of ITS sequences contributed from two species M. puniceaa and M. quintuplinervia, i.e. the variation of ITS gene among M. x cookei, M. punicea and M. quintuplinervia is congruence with the Mendel's genetics law. Therefore, the molecular evidences indicate that M. x cookei is a hybrid origin from M. punicea and M. quintuplinervia.
Yuan
Chang-Chun
CC
Life Science and Chemistry School, Zhanjiang Normal University, Zhanjiang 524048, China. yuancc@zhjnc.edu.cn
Li
Pei-Xin
PX
Wang
Yan-Fang
YF
Shi
Su-Hua
SH
D001483:Base Sequence; D002678:Chimera; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D008969:Molecular Sequence Data; D028101:Papaveraceae / Q000235:genetics*
D021903:DNA, Ribosomal Spacer