Potassium assay in blastomere of two-cell mouse embryo after equilibration and washing from cryoprotector.
The need for effective methods of cryopreservation of early mammalian embryos necessitates the study of the mechanisms of blastomere adaptation to the equilibration procedure and subsequent washing from the cryoprotector. The osmotic effects during these procedures can cause electrolyte imbalance in embryonic cells. Intracellular potassium concentrations at the stage of two-blastomere mouse embryo were studied by electron probe microanalysis.
Gol'dshtein
D V
DV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
Smolyaninova
E I
EI
Pogorelov
A G
AG
D000818:Animals; D001757:Blastomeres / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D004622:Embryo, Mammalian; D047108:Embryonic Development; D019855:Ethylene Glycol / Q000494:pharmacology*; D005612:Freeze Drying; D051379:Mice; D008815:Mice, Inbred Strains; D011188:Potassium / Q000032:analysis* / Q000502:physiology; D014867:Water / Q000737:chemistry
D003451:Cryoprotective Agents; D014867:Water; D019855:Ethylene Glycol; D011188:Potassium