Determination of phenolic acids in olive oil by capillary electrophoresis.
A CZE method for the separation and quantitation of phenolic acids (cinnamic, syringic, p-coumaric, vanillic, caffeic, 3,4-dihydroxyphenylacetic, protocatechuic), extracted from extra virgin olive oil, was developed. The sample preparation involved the LLE and SPE extraction methods. CE separation was performed in a fused silica capillary of I.D.= 50microm using as a BGE 40 mM borate buffer at pH=9.2. The separation voltage was 18kV with corresponding current of 27-28 microA. Detection was accomplished with UV-detector at lambda=200nm. The proposed method was fully validated. A good repeatability of migration time (RSD% ranged from 0.81 to 1.63) and of corrected peak area (RSD% from 2.89 to 5.77) was obtained. The linearity of detector response in the range from 5 to 50 ppm was checked, obtaining the correlation coefficient R2 values in the range: 0.9919-0.9997. Some phenolic acids in real oil samples were detected and quantified with the proposed method.
Buiarelli
Francesca
F
Dipartimento di Chimica, Università La Sapienza, P le A Moro 5, 00185 Roma, Italy.
Di Berardino
Sonia
S
Coccioli
Franco
F
Jasionowska
Renata
R
Russo
Mario Vincenzo
MV
D019075:Electrophoresis, Capillary; D062385:Hydroxybenzoates / Q000032:analysis*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D062385:Hydroxybenzoates; D000069463:Olive Oil; D010938:Plant Oils; C017616:phenolic acid