Solid-phase microextraction-gas chromatography-mass spectrometric analysis of volatile compounds in a compounded Chinese medicinal prescription, Xiao-Cheng-Qi-Tang.
In this paper, a headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was applied for the analysis of volatile compounds in a famous compounded Chinese medicinal prescription, Xiao-Cheng-Qi-Tang. Some parameters affecting the extraction efficiency such as stirring, extraction temperature, fiber exposure time and desorption time were optimized. The best results were obtained using a 100 microm PDMS fiber during headspace extraction at 90 degrees C with stirring at 1000 rpm for 20 min. Twenty-seven compounds were identified in Xiao-Cheng-Qi-Tang including some main compounds such as d-limonene and linalool. Inter- and intra-day relative standard deviations (R.S.Ds.) were less than 15.6%, showing that the method had a good reproducibility. The result might provide some foundation for building the convincing theory on the pharmacological activity of this prescription.
Sha
Yunfei
Y
Department of Pharmaceutical Analysis, Fudan University, 138 Yixueyuan Raod, Shanghai 200032, PR China.
Shen
Shun
S
Duan
Gengli
G
D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012996:Solutions; D013696:Temperature
D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D012996:Solutions; C499893:xiao-cheng-qi-tang