[Study on the changes of IL-3 and its receptor in mice with immune-mediated aplastic anemia].
The aim of this study was to find new idea for clinical treatment of aplastic anemia. Immune-mediated aplastic anemia mice were developed, IL-3 in the supernatant with PHA stimulating splenic cells was detected by ELISA, semi-quantiting analysis of IL-3R was performed by point hybridization. The results showed that the IL-3 level in the supernatant with PHA stimulating splenic cells of immune-mediated aplastic anemia mice was higher than controls, difference between them was significant (P <0.001), while amount of IL-3 receptor by semi-quantiting analysis was lower than control significantly. In conclusion, the IL-3 receptor expression level is important for pathogenesis and treatment strategy of aplastic anemia.
Huang
Tao
T
Department of Hematology, The Yue Yang Iraditional Chinese and Weseten Medicine Integrated Hospital Affiliated to Shanghai Traditional Chinese University, Shanghai 200437, China. 13331993136@133sh.com
Huang
Zhen-Qiao
ZQ
Zhou
Yong-Ming
YM
Xue
Zhi-Zhong
ZZ
Gan
Ren-Bao
RB
D000741:Anemia, Aplastic / Q000276:immunology* / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D007377:Interleukin-3 / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis* / Q000235:genetics
D007377:Interleukin-3; D012333:RNA, Messenger; D016185:Receptors, Interleukin-3