Genotyping of single nucleotide polymorphisms (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.
Using competitive allele-specific oligonucleotide hybridization with immunochromatographic strip (CASSOH), we have developed a simplified method for the detection of eight polymorphisms that are especially important in the identification of drug responders or non-responders and patients at increased risk of drug toxicity. The genotyping method is unambiguously determined by the presence or the absence of visible purple lines on the immunochromatographic strip, and results are obtained within 5 min after PCR. This method is rapid, highly sensitive, simplified, and should be suitable for point-of-care genotyping in clinical settings.
Hiratsuka
Masahiro
M
Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University, Sendai, Japan. mhira@tohoku-pharm.ac.jp
Ebisawa
Aiko
A
Matsubara
Yoichi
Y
Kure
Shigeo
S
Konno
Yumiko
Y
Sasaki
Takamitsu
T
Mizugaki
Michinao
M
D000483:Alleles; D002845:Chromatography / Q000379:methods; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D015345:Oligonucleotide Probes / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D015345:Oligonucleotide Probes