Characterization of a highly inducible novel CC chemokine from differentiated rainbow trout (Oncorhynchus mykiss) macrophages.
A full-length cDNA clone encoding a novel trout CC chemokine was identified in expressed sequence tags generated from lipopolysaccharide (LPS)-stimulated in vitro differentiated macrophages isolated from the head kidney of the rainbow trout (Oncorhynchus mykiss). The putative 101-amino-acid protein is 38% similar to Macaca mulatta CCL4 (macrophage inflammatory protein 1beta) but is also similar to several other related mammalian CC chemokines, including human Act-2. Real-time PCR and conventional RT-PCR revealed significant up-regulation of transcript levels of the trout CCL4-like mRNA in LPS-stimulated in vitro differentiated macrophages. In unstimulated trout, CCL4-like mRNA expression was detected at different levels in all tissues tested, whereas in LPS-challenged animals (6 mg/kg), CCL4-like mRNA increased in intestine, ovary and spleen at both 24 h and 72 h post-injection. In gills, CCL4-like mRNA expression was inhibited after LPS administration. Based on the highly regulated expression pattern exhibited by the trout CCL4-like mRNA, it is likely that this chemokine plays an important regulatory role in the immune response of trout.
Mackenzie
S
S
Unitat de Fisiologia Animal, Departament de Biologia Cellular, Fisiologia i d'Immunologia, Facultat de Ciencies, Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Spain. simon.mackenzie@uab.es
Liarte
C
C
Iliev
D
D
Planas
J V
JV
Tort
L
L
Goetz
F W
FW
D000595:Amino Acid Sequence; D000818:Animals; D019742:Chemokines, CC / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000276:immunology*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis
D019742:Chemokines, CC; D012333:RNA, Messenger