title: Organ-specific toxicokinetics and dose-response of arsenic in tilapia Oreochromis mossambicus.
abstract: We appraised organ-specific toxicokinetics and dose responses of arsenic burdens in tilapia Oreochromis mossambicus. We kinetically linked an Area-under-the-curve (AUC)-based acute toxicity model and a pharmacodynamic model to derive dose-response relationships between equilibrium organ-specific arsenic concentrations and mortality effects. The AUC-based acute toxicity model was also used to derive organ-specific internal effect concentration (IEC)-time-response relationships, which can also be applied to predict a time-mortality profile. We conducted a 7-day exposure experiment to obtain toxicokinetic parameters, whereas the AUC-based acute toxicity model was verified with LC50(t) data obtained from a 7-day acute toxicity bioassay. Our results demonstrated that 96-hour LC50 and incipient LC50 for tilapia exposed to arsenic are 28.68 (95% confidence interval to 24.92 to 32.44) and 25.55 mg L(-1), respectively. Dose-response relationships followed the Hill equation, which could be expressed as organ-specific bioconcentration factors and incipient LC50. Organ-specific dose-response relationships showed that muscle, gill, and liver have a relatively steep sigmoid dose-response profile in that IEC50 were 26.6, 62.5, and 78.5 microg g(-1) dry wt (dw), respectively. Organ-specific arsenic internal lethal burdens were the highest in the gill and the lowest in the muscle in waterborne-exposed tilapia. The IEC and target-organ concentrations derived in this study can be used in site-specific risk assessment.
authors.affiliation: Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, 10617, China. cmliao@ntu.edu.tw
authors.forename: C M
authors.initials: CM
authors.lastname: Liao
authors.forename: J W
authors.initials: JW
authors.lastname: Tsai
authors.forename: M P
authors.initials: MP
authors.lastname: Ling
authors.forename: H M
authors.initials: HM
authors.lastname: Liang
authors.forename: Y H
authors.initials: YH
authors.lastname: Chou
authors.forename: P T
authors.initials: PT
authors.lastname: Yang
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D005880:Gills / Q000473:pathology; D007700:Kinetics; D007928:Lethal Dose 50; D008099:Liver / Q000473:pathology; D018482:Muscle, Skeletal / Q000473:pathology; D017210:Tilapia; D014018:Tissue Distribution; D014873:Water Pollutants / Q000633:toxicity*
chemical_list: D014873:Water Pollutants; D001151:Arsenic