Synthesis of an artificial hole-transporting nucleoside triphosphate, dMDATP, and its enzymatic incorporation into DNA.
We report on the enzymatic synthesis of DNA wires by successive incorporation of artificial nucleotides. We synthesized an efficient hole-transporting nucleoside triphosphate, d(MD)ATP, and have examined the screening of polymerases and reverse transcriptases for enzymatic incorporation of d(MD)ATP into DNA. We found that KOD Dash is an effective enzyme for the enzymatic synthesis of (MD)A-containing DNA. Furthermore, the efficiency of enzymatic incorporation was remarkably improved by the addition of manganese chloride. By applying the PCR method to the synthesis of DNA containing a run of (MD)A, the (MD)A run was amplified up to 6 x 10(6) times. (MD)A runs prepared by the enzymatic method exhibited a high hole-transporting ability.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan. okamoto@sbchem.kyoto-u.ac.jp
Tanaka
Kazuo
K
Nishiza
Ken-ichiro
K
Saito
Isao
I
D000255:Adenosine Triphosphate / Q000138:chemical synthesis* / Q000235:genetics; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000138:chemical synthesis* / Q000235:genetics; D009705:Nucleosides / Q000138:chemical synthesis* / Q000235:genetics
D009705:Nucleosides; D000255:Adenosine Triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase