Antigenic differences between apo-B in native and circulating modified low-density lipoproteins.
The state of apo-B in native and circulating modified low-density lipoproteins was studied by solid-phase enzyme immunoassay. We studied the interaction of these particles with monoclonal antibodies to apo-B of low-density lipoproteins. Native and circulating modified low-density lipoproteins had different affinity for the studied antigens. Our results illustrate conformational changes in apo-B of circulating modified low-density lipoproteins compared to native low-density lipoproteins. These changes probably contribute to increased accumulation of particles in vascular cells and their transformation into foam cells giving way to atherosclerotic vascular lesions.
Suprun
I V
IV
Laboratory for Mechanisms of Atherogenesis, Institute of Experimental Cardiology, Russian Research-and-Production Complex for Cardiology, Russian Ministry of Health, Moscow. inat@cardio.ru
Mel'nichenko
A A
AA
Yanushevskaya
E V
EV
Vlasik
T N
TN
Sobenin
I A
IA
Panasenko
O M
OM
Orekhov
A N
AN
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001055:Apolipoproteins B / Q000276:immunology* / Q000378:metabolism; D001161:Arteriosclerosis / Q000209:etiology / Q000276:immunology / Q000473:pathology; D006801:Humans; D007118:Immunoassay; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL