title: Transcriptome profiling in root nodules and arbuscular mycorrhiza identifies a collection of novel genes induced during Medicago truncatula root endosymbioses.
abstract: Transcriptome profiling based on cDNA array hybridizations and in silico screening was used to identify Medicago truncatula genes induced in both root nodules and arbuscular mycorrhiza (AM). By array hybridizations, we detected several hundred genes that were upregulated in the root nodule and the AM symbiosis, respectively, with a total of 75 genes being induced during both interactions. The second approach based on in silico data mining yielded several hundred additional candidate genes with a predicted symbiosis-enhanced expression. A subset of the genes identified by either expression profiling tool was subjected to quantitative real-time reverse-transcription polymerase chain reaction for a verification of their symbiosis-induced expression. That way, induction in root nodules and AM was confirmed for 26 genes, most of them being reported as symbiosis-induced for the first time. In addition to delivering a number of novel symbiosis-induced genes, our approach identified several genes that were induced in only one of the two root endosymbioses. The spatial expression patterns of two symbiosis-induced genes encoding an annexin and a beta-tubulin were characterized in transgenic roots using promoter-reporter gene fusions.
authors.affiliation: Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Manthey
authors.forename: Franziska
authors.initials: F
authors.lastname: Krajinski
authors.forename: Natalija
authors.initials: N
authors.lastname: Hohnjec
authors.forename: Christian
authors.initials: C
authors.lastname: Firnhaber
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Perlick
authors.forename: Helge
authors.initials: H
authors.lastname: Küster
mesh_terms: D017302:Annexins / Q000235:genetics; D005658:Fungi / Q000254:growth & development; D020869:Gene Expression Profiling; D017343:Genes, Plant; D046913:Medicago truncatula / Q000235:genetics* / Q000382:microbiology*; D038821:Mycorrhizae / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D013559:Symbiosis; D014404:Tubulin / Q000235:genetics
chemical_list: D017302:Annexins; D014404:Tubulin