[Study on the interface of human hepatocyte/micropore polypropylene ultrafiltration membrane].
OBJECTIVE
To found a new interface of human hepatocyte/micropore polypropylene ultrafiltration membrane (MPP) with good cytocompatibility so as to construct bioartificial bioreactor with polypropylene hollow fibers in future.


METHODS
MPP ultrafiltration membrane underwent chemical grafting modification through ultraviolet irradiation and Fe(2+) reduction. The contact angles of MPP and the modified MPP membranes were measured. Human hepatic cells L-02 were cultured. MPP and modified MPP membranes were spread on the wells of culture plate and human hepatic cells and cytodex 3 were inoculated on them. Different kinds of microscopy were used to observe the morphology of these cells.


RESULTS
The water contact angle of MPP and the modified MPP membranes decreased from 78 degrees +/- 5 degrees to 27 degrees +/- 4 degrees (P < 0.05), which indicated that the hydrophilicity of the membrane was improved obviously after the grafting modification. Human hepatocyte L-02 did not adhere to and spread on the modified MPP membrane surface, and only grew on the microcarrier cytodex 3 with higher density and higher proliferation ratio measured by MTT.


CONCLUSIONS
Grafting modification of acrylamide on MPP membrane is a good method to improve the human hepatocyte cytocompatibility with MPP ultrafiltration membrane.
Peng
Cheng-Hong
CH
Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Han
Bao-San
BS
Gao
Chang-You
CY
Ma
Zu-Wei
ZW
Zhao
Zhi-Ming
ZM
Wang
Yong
Y
Liu
Hong
H
Zhang
Gui-di
GD
Yang
Mei-Juan
MJ
D021522:Bioartificial Organs; D019149:Bioreactors; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D006801:Humans; D017114:Liver Failure, Acute; D019164:Liver, Artificial; D008567:Membranes, Artificial; D010539:Permeability; D011126:Polypropylenes / Q000737:chemistry; D013499:Surface Properties; D013500:Surface Tension; D023822:Tissue Engineering / Q000379:methods; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods; D014508:Urea / Q000378:metabolism
D008567:Membranes, Artificial; D011126:Polypropylenes; D014508:Urea