Recombinant Sendai viruses with L1618V mutation in their L polymerase protein establish persistent infection, but not temperature sensitivity.
The Sendai virus pi strain (SeVpi) isolated from cells persistently infected with SeV shows mainly two phenotypes: (1) temperature sensitivity and (2) an ability of establishing persistent infection (steady state). Three amino acid substitutions are found in the Lpi protein and are located at aa 1088, 1618, and 1664. Recombinant SeV(Lpi) (rSeV(Lpi)) having all these substitutions is temperature sensitive and is capable of establishing persistent infection (steady state). rSeVs carrying the fragment containing L1618V show both phenotypes. rSeV(L1618V), in which leucine at aa 1618 is replaced with valine, has the ability of establishing persistent infection, but is not a temperature-sensitive mutant, indicating that the ability of a virus to establish persistent infection can be separated from temperature sensitivity. The amino acid change at 1618(L-->V) coexisting with aa 1169 threonine is required for acquirement of a temperature-sensitive phenotype. Three amino acid substitutions are also found in the Ppi protein, but rSeV(Ppi) does not show these phenotypes.
Nishio
Machiko
M
Department of Microbiology, Mie University School of Medicine, Mie-Prefecture 514-8507, Japan.
Nagata
Ai
A
Tsurudome
Masato
M
Ito
Morihiro
M
Kawano
Mitsuo
M
Komada
Hiroshi
H
Ito
Yasuhiko
Y
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D006389:Hemagglutinins, Viral / Q000032:analysis; D007930:Leucine / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics; D011995:Recombination, Genetic; D029082:Sendai virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016415:Sequence Alignment; D013696:Temperature; D014633:Valine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D006389:Hemagglutinins, Viral; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D014764:Viral Proteins; C064365:L protein, Sendai virus; D012321:DNA-Directed RNA Polymerases; D007930:Leucine; D014633:Valine