[Gene inactivation in Mycobacterium tuberculosis and its use in tuberculosis control and prevention].
Availability of the M. tuberculosis genome sequence and the development of sophisticated systems for genetic manipulation of bacilli offer the potential for new and effective tools to prevent and control tuberculosis. Efficient methods to inactivate mycobacterial genes have been developed. These methods have become the cornerstone for the application and development of mycobacterial functional genomics. Specific mutants are generated to establish the role of targetted genes associated with mycobacterial physiology and pathogenesis. Gene inactivation, supported directly or indirectly by the deciphering of the mycobacterial genome, has permitted the generation of large numbers of M. tuberculosis mutants. Analysis of these mutants has (in some cases) established relationships between gene products and their role in mycobacterial physiology and pathogenesis.
Chacón
Ofelia
O
Unidad de Bacteriología y Micobacterias, Corporación para Investigaciones Biológicas, Medellín, Colombia.
Realpe
Teresa
T
Barletta
Raúl Gerardo
RG
Robledo
Jaime
J
D000818:Animals; D020868:Gene Silencing; D005798:Genes, Bacterial / Q000502:physiology; D005821:Genetic Techniques; D006801:Humans; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D014376:Tuberculosis / Q000382:microbiology* / Q000517:prevention & control*