[Effect of jiantai liquid on expression of estrogen and progesterone receptors in uterus of mice with embryo implantation dysfunction induced by mifepristone].
OBJECTIVE
To explore the molecular mechanism of jiantai liquid (JTL) in improving endometrial receptivity of mice with embryo implantation dysfunction (EID).


METHODS
Mice model of EID induced by mifepristone were intervened with JTL (Twig of Chinese Taxillus, Red Sage root, Chinese Angelica, Milkvetch root, Chuanxiong rhizome), and sacrificed on day 8 of pregnancy. The endometrial estrogen receptor (ER) and progesterone receptor (PR) protein and their gene expressions were assessed by Western blot and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
Levels of ER and PR protein and their gene expressions in the JTL treated group were significantly higher than those in the model group respectively (all P < 0.05), and showed insignificant difference from those in the normal control group (all P > 0.05).


CONCLUSIONS
JTL could promote the development of endometrium and improve the embryo implantation by way of regulating the levels of ER and PR protein and gene expression in mice with EID.
Liu
Yan-juan
YJ
Institute of Integrated Traditional Chinese & Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan (430030). tjyjliu@hotmail.com
Huang
Guang-ying
GY
Yang
Ming-wei
MW
Gong
Ping
P
Lu
Fu'er
F
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects; D005260:Female; D008186:Luteolytic Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D015735:Mifepristone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D011980:Receptors, Progesterone / Q000096:biosynthesis* / Q000235:genetics; D014599:Uterus / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D008186:Luteolytic Agents; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D015735:Mifepristone