Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system.
BACKGROUND
The utilization of cryopreserved red blood cell (RBC) units had been limited by a maximum postdeglycerolization storage of 24 hours at 1 to 6 degrees C until the recent development of a closed system for the glycerolization and deglycerolization process.


METHODS
Sixty leukoreduced additive solution (AS), AS-1 (n = 30) and AS-3 (n = 30) RBC units from 500-mL whole blood (WB) collections were stored for 6 days, glycerolized, frozen at -70 +/- 5 degrees C for at least 14 days, thawed, deglycerolized, and stored for 15 days at 1 to 6 degrees C. Glycerolization and deglycerolization were performed with the ACP 215. In-vitro variables were tested before glycerolization, on Day 0, and Day 15 after deglycerolization storage. Forty donors were assessed for double-label 24-hour percent recovery, and T1/2 survival time was measured for 20 donors.


RESULTS
Postdeglycerolization mean +/- standard deviation in-vitro RBC mass recoveries were 93 +/- 5 percent for AS-1 and 95 +/- 4 percent for AS-3. Mean hemoglobin +/- standard deviation after deglycerolization was 50.5 +/- 5.5g for AS-1 and 50.1 +/- 3.5g for AS-3. Mean hemolysis (Day 15) was 0.36 +/- 0.11 percent for AS-1 and 0.38 +/- 0.13 percent for AS-3. Double-label 24-hour in-vivo recoveries were 82.5 +/- 7.8 percent for AS-1 and 81.4 +/- 7.1 percent for AS-3. The 51Cr T1/2 value was 41.8 +/- 3.97 for AS-1 and 40.6 +/- 7.11 for AS-3. Other in-vitro variables were as expected.


CONCLUSIONS
Leukoreduced AS-1 and AS-3 RBCs after frozen storage at -70 +/- 5 degrees C can be stored for up to 14 days when processing is performed with the ACP 215 system with resuspension of deglycerolized RBCs in AS-3.
Bandarenko
Nicholas
N
University of North Carolina, Chapel Hill, USA. nbandare@unch.unc.edu
Hay
Shauna N
SN
Holmberg
Jerry
J
Whitley
Pam
P
Taylor
Harry L
HL
Moroff
Gary
G
Rose
Leslie
L
Kowalsky
Richard
R
Brumit
Marla
M
Rose
Michael
M
Sawyer
Sherrie
S
Johnson
Adrienne
A
McNeil
Deanna
D
Popovsky
Mark A
MA
D000225:Adenine; D001781:Blood Component Removal; D001793:Blood Preservation; D002951:Citrates; D015925:Cryopreservation / Q000379:methods*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes; D005947:Glucose; D005990:Glycerol / Q000008:administration & dosage*; D006801:Humans; D007962:Leukocytes; D008353:Mannitol; D010710:Phosphates; D012965:Sodium Chloride; D013997:Time Factors
C037839:ADSOL; C058647:AS-3 preservative solution; D002951:Citrates; D010710:Phosphates; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; D005990:Glycerol