Construction and immunogenicity of human papillomavirus type 6b L1 recombinant plasmid.
OBJECTIVE
To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immunogenicity in mice.


METHODS
The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunogenicity study.


RESULTS
The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice's sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-gamma were increased in the same mice.


CONCLUSIONS
HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata.
Liu
Fang
F
Department of Dermatology, Beijing Chaoyang Hospital, Beijing, 100020. roseliufang@sohu.com
Wang
Jia-bi
JB
Zuo
Ya-gang
YG
Liu
Yue-hua
YH
Ma
Dong-lai
DL
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019556:COS Cells; D036022:Capsid Proteins; D002522:Chlorocebus aethiops; D003218:Condylomata Acuminata / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D019444:Vaccines, DNA / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D007376:Interleukin-2; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma