title: Genetic and physical map of the pLAFR1 vector.
abstract: This paper presents the complete sequencing and annotation of the pLAFR1 vector. pLAFR is a tetracycline-resistant "cosmid" cloning vector, which is derived from the 20 kb plasmid pRK290, a RK2-derivative. Due to its broad host range, the pLAFR1 vector has been widely used in the genetic analysis of a broad number of gram-negative bacterial species. The availability of the complete pLAFR1 sequence will most definitely help in the construction and analysis of clone librares based on pRK290 or pLAFR vectors.
authors.affiliation: Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Heverlee, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Vanbleu
authors.forename: Kathleen
authors.initials: K
authors.lastname: Marchal
authors.forename: Jos
authors.initials: J
authors.lastname: Vanderleyden
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003360:Cosmids / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA