Fate of constitutive endocytic vesicles formed in the growth cone: transport of vesicles from one growth cone to another in the same neuron.
A developing neuron must have multiple paths of communication coordinating events among all its parts. One of these is the transport to the cell body of endocytic vesicles formed in growth cones. In order to observe this at single cell resolution, we developed a technique in which the fluorescent dye FM1-43 was applied to a single growth cone and newly formed constitutive endocytic vesicles were labeled. Using low light, time-lapse microscopy we were able to follow the movement of these vesicles throughout the neuron. The vast majority of the transported vesicles went to the cell body. However, many were observed to enter secondary neurites and to be transported to other growth cones. These new, more direct paths of transport that link the multiple growth cones of a neuron may play a role in several important developmental events involving interactions between the multiple neurites of a single neuron.
Denburg
Jeffrey L
JL
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA. jeffrey-denburg@uiowa.edu
Hughen
Ronald W
RW
Tucker
Diane
D
Kater
Stanley B
SB
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003952:Diagnostic Imaging / Q000379:methods; D005728:Ganglia, Sympathetic / Q000166:cytology; D020439:Growth Cones / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods; D008954:Models, Biological; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013997:Time Factors; D022161:Transport Vesicles / Q000502:physiology*
C073804:FM1 43; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds