Determination of lipid oxidation level in broiler meat by liquid chromatography.
An assay was conducted for the determination of malondialdehyde (MDA) levels in broiler meat. The method involves extraction of tissues with trichloroacetic acid (TCA) and reaction of the TCA extract with 2,4-dinitrophenylhydrazine (DNPH). After separation of the MDA-DNPH complex using a solid-phase extraction C18 column, samples were eluted with 1 mL acetonitrile. Aliquots of 20 microL acetonitrile were analyzed by liquid chromatography on reversed-phase C18 column (3 microm) with UV detection. The products were eluted isocratically with the mobile phase containing acetonitrile-water-acetic acid (39 + 61 + 0.2, v/v/v). The retention time for MDA-DNPH was 6.5 min, and the detection limit was 3.5 microg/kg. Two extraction methods (cold and hot) were also used in the study. The results showed that hot extraction increased results about 55.8% and recovery from samples spiked with 116.6 microg/kg was lower (74.6%) in comparison with cold extraction (94.7%).
Marcincák
Slavomir
S
University of Veterinary Medicine, Department of Food Hygiene and Technology, Komenského 73, 041 81 Kosice, The Slovak Republic. marcincak@lycos.com
Sokol
Jozef
J
Bystrický
Pavel
P
Popelka
Peter
P
Turek
Peter
P
Bhide
Mangesh
M
Máté
Dionýz
D
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D050356:Lipid Metabolism; D008315:Malondialdehyde / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D010084:Oxidation-Reduction
D008315:Malondialdehyde