title: Activation of the discoidin domain receptor 2 induces expression of matrix metalloproteinase 13 associated with osteoarthritis in mice.
abstract: Human genetic studies indicate that mutations in type IX and XI collagens result in early-onset osteoarthritis (OA) with a wide spectrum of osteochondrodysplasia. However, a convincing causal chain of events underlying the role of these collagen mutations in the pathogenesis of OA has not been elucidated. Here we show that the expression of a cell surface collagen receptor, discoidin domain receptor 2 (DDR2), is increased in chondrocytes of the articular cartilage of knee joints in mice that develop OA as a result of a heterozygous mutation in type XI collagen. At the same time point, 6 months, we also found increased expression and activity of matrix metalloproteinase 13 (MMP-13) in the mutant mouse knee cartilage. The expression of both DDR2 and MMP-13 was increased in chondrocytes cultured on plates coated with native type II collagen but not on gelatin, and overexpression of DDR2, but not of a truncated form, was found to induce the expression of MMP-13 when chondrocytes were cultured on type II collagen but not on plastic. The DDR2-induced expression of MMP-13 appears to be specific, since we did not observe induction of MMP-1, MMP-3, MMP-8, ADAMTS-4, ADAMTS-5, and IL-1 transcripts in human chondrocytes or Mmp-3, Mmp-8, Adamts-4, Adamts-5, and Il-1 in mouse chondrocytes. Our data suggest that the defect in the cartilage matrix of mice that are heterozygous for a type XI collagen mutation (cho/+) permits activation and up-regulation of DDR2 in chondrocytes. This could be due to increased exposure of chondrocytes to type II collagen as a result of the decreased amount of type XI collagen in the mutant cartilage. The specific induction of MMP-13 by DDR2 in response to its cartilage-specific ligand, type II collagen, may contribute to cartilage damage in hereditary OA.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Haibing
authors.initials: H
authors.lastname: Peng
authors.forename: Dongying
authors.initials: D
authors.lastname: Wu
authors.forename: Kenpan
authors.initials: K
authors.lastname: Hu
authors.forename: Mary B
authors.initials: MB
authors.lastname: Goldring
authors.forename: Bjorn R
authors.initials: BR
authors.lastname: Olsen
authors.forename: Yefu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D024043:Collagen Type II / Q000378:metabolism; D024161:Collagen Type XI / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism*; D000070818:Discoidin Domain Receptors; D004789:Enzyme Activation; D004790:Enzyme Induction; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology; D008024:Ligands; D053509:Matrix Metalloproteinase 13; D051379:Mice; D020370:Osteoarthritis, Knee / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000378:metabolism*
chemical_list: D024043:Collagen Type II; D024161:Collagen Type XI; D008024:Ligands; D011975:Receptors, Mitogen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse