The cDNA for leucomyosuppressin in Blattella germanica and molecular evolution of insect myosuppressins.
Myosuppressins are a group of 10-residues FMRFamide-related peptides reported in Dictyoptera, Orthoptera, Lepidoptera and Diptera. Myosuppressins inhibit visceral muscle contractions and, in the cockroach Blattella germanica, inhibit food intake. In B. germanica, the cDNA of leucomyosuppressin (LMS) has been cloned and sequenced. The deduced precursor is 96 amino acids long and contains a single copy of LMS. Brain mRNA levels remain constant during the first reproductive cycle of adult females, whereas those in the gut show a slight decline during the time of maximal food intake. Comparison of myosuppressin precursors of different species reveals that all have the same organization. Phylogenetic analysis suggests that the precursor experienced an accelerated evolution in Lepidoptera and Diptera with respect to Dictyoptera, whereas only Lepidoptera has radical changes in the bioactive peptide.
Vilaplana
Lluïsa
L
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
Castresana
José
J
Bellés
Xavier
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020048:Blattellidae / Q000235:genetics*; D001923:Brain Chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D007301:Insect Hormones / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D007313:Insecta / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007301:Insect Hormones; D009479:Neuropeptides; D011498:Protein Precursors; D012333:RNA, Messenger; C058650:leucomyosuppressin; D012313:RNA