title: New structures of the O-specific polysaccharides of proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose.
abstract: The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods, mass spectrometry, including MS/MS, and NMR spectroscopy, including experiments run in an H2O/D2O mixture to reveal correlations for NH protons. The O-polysaccharides were found to contain N-carboxyacetyl (malonyl) and N-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, Qui4N), respectively. The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: B
authors.initials: B
authors.lastname: Linder
authors.forename: R
authors.initials: R
authors.lastname: Fudala
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: H
authors.initials: H
authors.lastname: Moll
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: U
authors.initials: U
authors.lastname: Zähringer
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: C492812:4-amino-4,6-dideoxyglucose; D008070:Lipopolysaccharides; D019081:O Antigens; D005944:Glucosamine