Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing.
A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3'-end and a copper(II) complex to the 5'-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.
Brunner
Jens
J
Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Kraemer
Roland
R
D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods; D002413:Cations, Divalent; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D003300:Copper; D004247:DNA; D019793:Fluorescein