Gene expression analysis of peripheral T cells in a subgroup of common variable immunodeficiency shows predominance of CCR7(-) effector-memory T cells.
Common variable immunodeficiency (CVID) represents a heterogeneous group of antibody deficiency syndromes, characterized by defective antibody production in which T cell deficiency may play a pathogenic role. A subgroup of CVID patients has impaired in vitro T cell proliferation. Using microarray analyses of T cells from these patients, we found a gene expression pattern different from healthy controls and patients with X-linked agammaglobulinaemia. The profile of the differentially expressed genes suggests enhanced cytotoxic effector functions, antigen experienced or chronically activated T cells and a predominance of CCR7(-) T cells. Further experiments using flow cytometry revealed a striking predominance of CCR7(-) T cells in a subgroup of CVID patients, and an association with impaired T cell proliferation. Our observations indicate that a predominance of CCR7(-) T cells with effector-memory cell features and with reduced proliferative capacity may characterize a subgroup of CVID.
Holm
A M
AM
Section for Clinical Immunology and Infectious Diseases, Medical Department, The National Hospital, Oslo, Norway. a.m.holm@klinmed.uio.no
Sivertsen
E A
EA
Tunheim
S H
SH
Haug
T
T
Bjerkeli
V
V
Yndestad
A
A
Aukrust
P
P
Frøland
S S
SS
D000328:Adult; D000361:Agammaglobulinemia / Q000235:genetics / Q000276:immunology; D000368:Aged; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D041321:Chromosomes, Human, X; D017074:Common Variable Immunodeficiency / Q000235:genetics / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D008040:Genetic Linkage / Q000235:genetics / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine