title: Starch microparticles as oral vaccine adjuvant: antigen-dependent uptake in mouse intestinal mucosa.
abstract: An oral vaccine formulation comprised of starch microparticles with conjugated antigens is being developed. In this report we have examined the uptake of such microparticles by the intestinal mucosa and examined whether the conjugated antigen can influence the uptake. Two model antigens were used: recombinant cholera toxin B subunit (rCTB), which is known to bind to the ubiquitous GM1-receptor, and human serum albumin (HSA) which is not known to have any specific binding properties. The uptake was studied in mouse ligated intestinal loops into which the microparticles were injected. The intestinal loops were excised, fixed in ice-cold 95% ethanol. Entire specimens were mounted, exposed to fluorescence-labeled reagents staining the cytoskeleton, the particles and/or M cells and examined in a confocal laser-scanning microscope. A qualitative difference in the uptake of the rCTB- and HSA-conjugated microparticles was seen. The rCTB-conjugated microparticles were found both in villi and in the follicles of the Peyer's patches. HSA-conjugated microparticles could only be detected in the follicles of the Peyer's patches and not in villi. The rCTB conjugated to the microparticles did not lose its ability to bind the GM1-receptor, as shown with a GM1-ELISA, and the uptake of rCTB-conjugated microparticles in villi is most probably facilitated by the rCTB binding to the GM1-receptor. The qualitative difference in uptake could be of importance for the development of an immune response as the cytokine and chemokine microenvironment during antigen presentation will decide the differentiation of the immune response induced.
authors.affiliation: Department of Pharmacy, Uppsala University, Sweden.
authors.forename: Agneta
authors.initials: A
authors.lastname: Larhed
authors.forename: Linda
authors.initials: L
authors.lastname: Stertman
authors.forename: Emma
authors.initials: E
authors.lastname: Edvardsson
authors.forename: Ingvar
authors.initials: I
authors.lastname: Sjöholm
mesh_terms: D000276:Adjuvants, Immunologic / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D002772:Cholera Toxin / Q000008:administration & dosage / Q000493:pharmacokinetics; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics; D013213:Starch / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D018074:Vaccines, Conjugate / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D012709:Serum Albumin; D018074:Vaccines, Conjugate; D013213:Starch; D002772:Cholera Toxin