title: Effects of a tryptic hydrolysate from bovine milk alphaS1-casein on hemodynamic responses in healthy human volunteers facing successive mental and physical stress situations.
abstract: BACKGROUND
  Preclinical results in rats have demonstrated anxiolytic-like effects of a tryptic bovine alphaS1-casein hydrolysate.
  
  
  OBJECTIVE
  We investigated the putative effects of this tryptic hydrolysate on systolic (SBP), diastolic (DBP) blood pressures, heart rate (HR) values and plasma cortisol concentrations (CC) in human healthy volunteers facing successive stress situations.
  
  
  METHODS
  The subjects were (double blind) randomly allocated to ingest three times, 12 hours apart, two capsules containing either 200 mg of alphaS1-casein hydrolysate (TS) or bovine skimmed milk powder as a placebo (CS). On the morning of the test day, a first blood sample for baseline measurement of CC was taken before the subjects were submitted to the Stroop test (ST) and, after a 30-min rest, to a Cold Pressor test (CPT). SBP, DBP, and HR were continuously recorded for 5 min before the ST and during each stress situation. A second blood sample was taken 15 min after the end of the CPT condition.
  
  
  RESULTS
  ST and ST + CPT combined test situations increased SBP, DBP and HR. The significant "Treatment x SBP" and "Treatment x DBP" interactions indicated the lower percentage changes in SBP and DBP of the TS. In addition, the results showed a significant decrease of the CC in the TS but not in the CS throughout the ST + CPT combined stress tests. HR remained stable in TS between the initial rest period and the CPT unlike what happened in CS.
  
  
  CONCLUSIONS
  On the basis of blood pressure and cortisol changes, these results suggest an antistress profile of this alphaS1-casein hydrolysate in human subjects.
authors.affiliation: ETAP-Applied Ethology 13, rue du Bois de la Champelle, 54500, Vandoeuvre-lès-Nancy, France. etap@etap-lab.com
authors.forename: Michaël
authors.initials: M
authors.lastname: Messaoudi
authors.forename: Catherine
authors.initials: C
authors.lastname: Lefranc-Millot
authors.forename: Didier
authors.initials: D
authors.lastname: Desor
authors.forename: Benoît
authors.initials: B
authors.lastname: Demagny
authors.forename: Lionel
authors.initials: L
authors.lastname: Bourdon
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects* / Q000502:physiology; D002364:Caseins / Q000008:administration & dosage* / Q000494:pharmacology; D004311:Double-Blind Method; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D008297:Male; D008892:Milk / Q000737:chemistry; D013312:Stress, Physiological / Q000503:physiopathology*
chemical_list: D002364:Caseins; C475729:casein hydrolysate; D006854:Hydrocortisone