title: Elemental and redox analysis of single bacterial cells by x-ray microbeam analysis.
abstract: High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.
authors.affiliation: Environmental Research Division and Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439-4843, USA. kemner@anl.gov
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Kemner
authors.forename: Shelly D
authors.initials: SD
authors.lastname: Kelly
authors.forename: Barry
authors.initials: B
authors.lastname: Lai
authors.forename: Joerg
authors.initials: J
authors.lastname: Maser
authors.forename: Edward J
authors.initials: EJ
authors.lastname: O'loughlin
authors.forename: Deirdre
authors.initials: D
authors.lastname: Sholto-Douglas
authors.forename: Zhonghou
authors.initials: Z
authors.lastname: Cai
authors.forename: Mark A
authors.initials: MA
authors.lastname: Schneegurt
authors.forename: Charles F
authors.initials: CF
authors.lastname: Kulpa
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Nealson
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D002118:Calcium / Q000032:analysis; D002857:Chromium / Q000032:analysis / Q000494:pharmacology; D004602:Elements; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000032:analysis; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D013052:Spectrometry, X-Ray Emission
chemical_list: D004602:Elements; D002857:Chromium; C074702:chromium hexavalent ion; D010758:Phosphorus; D002118:Calcium