title: Development of a safe oral Abeta vaccine using recombinant adeno-associated virus vector for Alzheimer's disease.
abstract: A new oral vaccine for Alzheimer's disease was developed using recombinant adeno-associated virus vector carrying Abeta cDNA (AAV/Abeta). Oral administration of the vaccine without adjuvant induced the expression and secretion of Abeta1-43 or Abeta1-21 in the epithelial cell layer of the intestine in amyloid precursor protein transgenic mice. Serum antibody levels were elevated for more than six months, while T cell proliferative responses to Abeta was not detected. Brain Abeta burden was significantly decreased compared to the control without inflammatory changes. This oral AAV/Abeta vaccine seems to be promising for prevention and treatment of Alzheimer's disease.
authors.affiliation: National Institute for Longevity Sciences, NCGG, 36-3 Gengo, Morioka, Obu City, Aichi 474-8522, Japan. hhara@nils.go.jp
authors.forename: Hideo
authors.initials: H
authors.lastname: Hara
authors.forename: Alon
authors.initials: A
authors.lastname: Monsonego
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yuasa
authors.forename: Kayo
authors.initials: K
authors.lastname: Adachi
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Takeda
authors.forename: Keikichi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Howard L
authors.initials: HL
authors.lastname: Weiner
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tabira
mesh_terms: D000284:Administration, Oral; D000544:Alzheimer Disease / Q000276:immunology / Q000517:prevention & control*; D023582:Alzheimer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000276:immunology / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D023582:Alzheimer Vaccines; D016229:Amyloid beta-Peptides; D015703:Antigens, CD; D017931:DNA Primers; D018076:DNA, Complementary