title: B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells.
abstract: B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.
authors.affiliation: Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Liqun
authors.initials: L
authors.lastname: Luo
authors.forename: Andrei I
authors.initials: AI
authors.lastname: Chapoval
authors.forename: Dallas B
authors.initials: DB
authors.lastname: Flies
authors.forename: Gefeng
authors.initials: G
authors.lastname: Zhu
authors.forename: Fumiya
authors.initials: F
authors.lastname: Hirano
authors.forename: Shengdian
authors.initials: S
authors.lastname: Wang
authors.forename: Julie S
authors.initials: JS
authors.lastname: Lau
authors.forename: Haidong
authors.initials: H
authors.lastname: Dong
authors.forename: Koji
authors.initials: K
authors.lastname: Tamada
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Flies
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D060887:B7 Antigens; D018122:B7-1 Antigen / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D002455:Cell Division / Q000276:immunology; D045744:Cell Line, Tumor; D002999:Clone Cells; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D018076:DNA, Complementary / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology*; D014162:Transfection
chemical_list: D000276:Adjuvants, Immunologic; D060887:B7 Antigens; D018122:B7-1 Antigen; C486038:Cd276 protein, mouse; D018076:DNA, Complementary; D018984:Epitopes, T-Lymphocyte