Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion.
The distinct and overlapping roles of adhesion molecules belonging to the selectin and integrin families control the rate of leukocyte adhesion to stimulated vascular endothelial cells under hydrodynamic shear flow. Crystal structures have appeared for some of these interactions which complement molecular biology experiments, and clarify the molecular mechanism of the receptor-ligand binding interactions. Binding affinity data have also appeared using surface plasmon resonance and single-molecule biophysics experiments. These studies confirm and extend the predictions of previous experiments carried out in parallel-plate flow chambers, and cone and plate viscometers. This review discusses the current state of understanding on how molecular bond formation rates coupled with cellular and hydrodynamic features regulate leukocyte binding to endothelial cells.
Neelamegham
Sriram
S
Department of Chemical and Biological Engineering, State University of New York, Buffalo, NY 14260, USA. neel@eng.buffalo.edu
D001665:Binding Sites; D001692:Biological Transport / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000502:physiology*; D007700:Kinetics; D036904:Leukocyte Rolling / Q000502:physiology; D007962:Leukocytes / Q000502:physiology*; D008024:Ligands; D008954:Models, Biological; D011485:Protein Binding; D019087:Selectins / Q000737:chemistry / Q000502:physiology*; D020349:Surface Plasmon Resonance / Q000379:methods
D015815:Cell Adhesion Molecules; D016023:Integrins; D008024:Ligands; D019087:Selectins