[Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus].
OBJECTIVE
To modify the structure of dehydroepiandrosterone (DHEA).


METHODS
Using hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.


RESULTS
(1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3, 17-dione (I); 6alpha-hydroxyandrost-4-ene-3, 17-dione (II); 6alpha, 17beta-dihydroxyandrost-4-ene-3-one (III); androst-4-ene-3, 6, 17-trione (IV) and 17beta-hydroxyandrost-4-ene-3-one (V).


CONCLUSIONS
It is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.
Liu
Ying
Y
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Cheng
Ke-di
KD
Zhu
Ping
P
Feng
Wen-hua
WH
Meng
Chao
C
Zhu
Hui-xin
HX
He
Hui-xia
HX
Ma
Xiao-jun
XJ
D000735:Androstenedione / Q000737:chemistry / Q000302:isolation & purification*; D000736:Androstenes / Q000737:chemistry / Q000302:isolation & purification*; D001711:Biotransformation; D003470:Culture Media; D003687:Dehydroepiandrosterone / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D018517:Plant Roots / Q000378:metabolism; D010946:Plants, Medicinal / Q000378:metabolism*; D019657:Solanaceae / Q000378:metabolism*; D046509:Tissue Culture Techniques
D000736:Androstenes; D003470:Culture Media; D000735:Androstenedione; D003687:Dehydroepiandrosterone; C018213:androst-4-ene-3,6,17-trione