Impact of oxygen concentration on embryonic development of mouse zygotes.
The aim of the present study was to examine the effect of culture under 5 and 20% oxygen on the development, differentiation and viability of zygotes and in-vivo-produced embryos at the 2-cell and 8-cell stages of development. First, zygotes collected in a common pool were cultured in 20% O2 for 0, 23, 46 and 95 h. Zygotes and in-vivo-produced embryos at the 2-cell and 8-cell stages of development were then cultured in 5 or 20% O2. The proportion of embryos reaching the compaction and blastocyst stages of development did not differ between groups regardless of the period of time embryos were cultured in 20% O2 or the stage at beginning of culture. Duration of culture under 20% O2 had a significant effect on total number of blastocyst cells. A stage-specific effect was observed on total and trophectoderm cell numbers in blastocysts resulting from the culture of zygotes and in-vivo-produced embryos under 20% O2. ICM and percent ICM development was significantly decreased by culture in 20% O2 at all stages examined. Oxygen concentration had no effect on implantation rate and fetal weights upon embryo transfer. However, transfer of zygotes grown to the blastocyst stage in 20% O2 resulted in a dramatic decrease in fetal development per blastocyst and fetal development per implantation. These results demonstrate that culture of F1 mouse zygotes in 20% O2 compromises the developmental potential of resultant blastocysts, which appear to be normal on morphological assessment.
Karagenc
L
L
Bahçeci Women Health Care Centre and German Hospital, Istanbul, Turkey.
Sertkaya
Z
Z
Ciray
N
N
Ulug
U
U
Bahçeci
M
M
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000378:metabolism; D004624:Embryo Transfer; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000008:administration & dosage* / Q000378:metabolism; D011247:Pregnancy; D046509:Tissue Culture Techniques; D015053:Zygote / Q000254:growth & development* / Q000378:metabolism*
D010100:Oxygen