title: [Peripheral blood CD34+-positive mononuclear cells participate in neovasculogenesis of human ovarian epithelial carcinoma].
abstract: OBJECTIVE
  To observe whether endothelial cell (EC) progenitors (CD(34)(+)-positive mononuclear cells) participated in neovasculogenesis of ovarian epithelial carcinoma through in vitro and in vivo experiments, and to explore the mechanism of tumor neovasculogenesis.
  
  
  METHODS
  CD(34)(+)-positive mononuclear cells were isolated from peripheral blood of ovarian epithelial carcinoma patients by means of magnetic beads coated with antibody to CD(34)(+), plated on culture dishes coated with human fibronectin in endothelium medium, and examined by using RT-PCR, fluorescence-activated cell sorting (FACS) and nitric oxide (NO) assay kit for the expression of EC lineage-markers. EC-like cells were labeled with DiI ex vivo, and injected into immunodeficiency mice model with transplanted hypodermic SKOV3 by caudal vein. After 4 - 6 weeks, the tumor was resected and examined by confocal microscopy, and immunohistochemistry.
  
  
  RESULTS
  In vitro, CD(34)(+)-positive mononuclear cells differentiated into ECs. In animal models of SKOV3, EC progenitors (CD(34)(+)-positive mononuclear cells) incorporated into sites of neovasculogenesis in tumor, 4 - 6 weeks later DiI-labeled cells incorporated into capillaries and small arteries.
  
  
  CONCLUSIONS
  The neovasculogenesis in human ovarian epithelial carcinoma involves angiogenesis and vasculogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, Obstetrics and Gynecologic Hospital of Fudan University, Shanghai 200011, China.
authors.forename: Feng-lian
authors.initials: FL
authors.lastname: Xu
authors.forename: You-ji
authors.initials: YJ
authors.lastname: Feng
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000097:blood*; D002277:Carcinoma / Q000098:blood supply* / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D010051:Ovarian Neoplasms / Q000098:blood supply* / Q000473:pathology
chemical_list: D018952:Antigens, CD34