Detection of antigen-antibody reaction using a fluorescent probe and its application to homogeneous competitive-type immunoassay for insulin.
The interaction of bovine insulin with anti-human insulin antibody (mAb) was examined using a fluorescent probe. The fluorescence intensity of fluoresceinthiocarbamyl (FTC)-insulin was increased by adding mAb, and the increase was saturated at 53% at a molar ratio of FTC-insulin to mAb of 2.0. Based on the change in fluorescence intensity, a standard curve of the homogeneous competitive-type immunoassay was constructed, and the detection range of insulin was found to be 50-400 nM.
Oneda
Hiroshi
H
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sankyo-ku, Kyoto 606-8502, Japan.
Inouye
Kuniyo
K
D000818:Animals; D000937:Antigen-Antibody Reactions; D002417:Cattle; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007328:Insulin / Q000031:analogs & derivatives* / Q000032:analysis*; D007330:Insulin Antibodies / Q000276:immunology*; D013050:Spectrometry, Fluorescence / Q000379:methods
D007328:Insulin; D007330:Insulin Antibodies; C028647:insulin, fluoresceinthiocarbamyl(B29)-