Lipid rafts clustering and signalling by listeriolysin O.
Listeriolysin O, the major virulent determinant of Listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. In the present study, we reveal its other facet, rafts clustering. By immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins CD14 and CD24, which normally exhibit uniform distribution on J774 cells, undergo clustering upon treatment with LLO. The non-raft marker transferrin receptor is unaffected by such treatment. Rafts clustering might explain the induction of tyrosine phosphorylation observed on LLO-treated cells.
Gekara
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NO
Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. nge@gbf.de
Weiss
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D015703:Antigens, CD / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000494:pharmacology*; D051927:CD24 Antigen; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006460:Hemolysin Proteins; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D021962:Membrane Microdomains / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010766:Phosphorylation; D015398:Signal Transduction; D014443:Tyrosine / Q000737:chemistry
D015703:Antigens, CD; D001427:Bacterial Toxins; D051927:CD24 Antigen; C497889:CD24 protein, human; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D014443:Tyrosine; D002784:Cholesterol; C038705:hlyA protein, Listeria monocytogenes