Influence of the mycotoxins alpha- and beta-zearalenol (ZOL) on regulators of cap-dependent translation control in pig endometrial cells.
The molecular mechanisms that control the mycotoxin-mediated effects in porcine endometrial cells are far from being completely understood. Recent results show that they could inhibit cell proliferation. Therefore, the present study investigated the effects of the mycotoxins alpha-zearalenol (alpha-ZOL) and beta-zearalenol (beta-ZOL) on a cellular level. Mainly, the abundance and phosphorylation state (activity) of the cell cycle-dependent kinases MAPK and Akt (PKB) and their potential targets eIF4E (eukaryotic initiation factor 4E) and 4E-BP1 (4E binding protein, eIF4E repressor protein) were investigated. The results show that alpha-ZOL has apparently only a slight influence on the phosphorylation state of MAP kinases, Akt and on eIF4E and 4E-BP1. In contrast, their phosphorylation was strongly reduced in beta-ZOL-treated cells in a concentration-dependent manner. Therefore, our results indicate that beta-ZOL potentially not only influences transcription but also effects gene expression on translational level. The effect of alpha- and beta-ZOL on endometrial cell proliferation and their toxicology are discussed.
Wollenhaupt
K
K
Unit of Reproductive Biology, Research Institute for the Biology of Farm Animals, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany. wollenhaupt@fbn-dummerstorf.de
Jonas
L
L
Tiemann
U
U
Tomek
W
W
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D039561:Eukaryotic Initiation Factor-4E / Q000096:biosynthesis / Q000235:genetics; D039642:Eukaryotic Initiation Factors; D005260:Female; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis / Q000235:genetics; D009183:Mycotoxins / Q000633:toxicity*; D010448:Peptide Initiation Factors / Q000096:biosynthesis* / Q000235:genetics; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D015029:Zeranol / Q000031:analogs & derivatives* / Q000633:toxicity*
D002352:Carrier Proteins; D039561:Eukaryotic Initiation Factor-4E; D039642:Eukaryotic Initiation Factors; D009183:Mycotoxins; D010448:Peptide Initiation Factors; D010750:Phosphoproteins; D012333:RNA, Messenger; C029659:zearalenol; D015029:Zeranol; D020929:Mitogen-Activated Protein Kinase Kinases