Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
Tetraodon nigroviridis is a freshwater puffer fish with the smallest known vertebrate genome. Here, we report a draft genome sequence with long-range linkage and substantial anchoring to the 21 Tetraodon chromosomes. Genome analysis provides a greatly improved fish gene catalogue, including identifying key genes previously thought to be absent in fish. Comparison with other vertebrates and a urochordate indicates that fish proteins have diverged markedly faster than their mammalian homologues. Comparison with the human genome suggests approximately 900 previously unannotated human genes. Analysis of the Tetraodon and human genomes shows that whole-genome duplication occurred in the teleost fish lineage, subsequent to its divergence from mammals. The analysis also makes it possible to infer the basic structure of the ancestral bony vertebrate genome, which was composed of 12 chromosomes, and to reconstruct much of the evolutionary history of ancient and recent chromosome rearrangements leading to the modern human karyotype.
Jaillon
Olivier
O
UMR 8030 Genoscope, CNRS and Université d'Evry, 2 rue Gaston Crémieux, 91057 Evry Cedex, France.
Aury
Jean-Marc
JM
Brunet
Frédéric
F
Petit
Jean-Louis
JL
Stange-Thomann
Nicole
N
Mauceli
Evan
E
Bouneau
Laurence
L
Fischer
Cécile
C
Ozouf-Costaz
Catherine
C
Bernot
Alain
A
Nicaud
Sophie
S
Jaffe
David
D
Fisher
Sheila
S
Lutfalla
Georges
G
Dossat
Carole
C
Segurens
Béatrice
B
Dasilva
Corinne
C
Salanoubat
Marcel
M
Levy
Michael
M
Boudet
Nathalie
N
Castellano
Sergi
S
Anthouard
Véronique
V
Jubin
Claire
C
Castelli
Vanina
V
Katinka
Michael
M
Vacherie
Benoît
B
Biémont
Christian
C
Skalli
Zineb
Z
Cattolico
Laurence
L
Poulain
Julie
J
De Berardinis
Véronique
V
Cruaud
Corinne
C
Duprat
Simone
S
Brottier
Philippe
P
Coutanceau
Jean-Pierre
JP
Gouzy
Jérôme
J
Parra
Genis
G
Lardier
Guillaume
G
Chapple
Charles
C
McKernan
Kevin J
KJ
McEwan
Paul
P
Bosak
Stephanie
S
Kellis
Manolis
M
Volff
Jean-Nicolas
JN
Guigó
Roderic
R
Zody
Michael C
MC
Mesirov
Jill
J
Lindblad-Toh
Kerstin
K
Birren
Bruce
B
Nusbaum
Chad
C
Kahn
Daniel
D
Robinson-Rechavi
Marc
M
Laudet
Vincent
V
Schachter
Vincent
V
Quétier
Francis
F
Saurin
William
W
Scarpelli
Claude
C
Wincker
Patrick
P
Lander
Eric S
ES
Weissenbach
Jean
J
Roest Crollius
Hugues
H
D000818:Animals; D001482:Base Composition; D002875:Chromosomes / Q000235:genetics*; D002877:Chromosomes, Human / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D020440:Gene Duplication; D005796:Genes / Q000235:genetics; D016678:Genome; D006801:Humans; D007621:Karyotyping; D008322:Mammals / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D020543:Proteome; D017422:Sequence Analysis, DNA; D026801:Synteny / Q000235:genetics; D014561:Urochordata / Q000235:genetics; D014714:Vertebrates / Q000235:genetics*
D020543:Proteome