Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis.
Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.
Verstraelen
Hans
H
Department of Obstetrics and Gynaecology, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
Verhelst
Rita
R
Claeys
Geert
G
Temmerman
Marleen
M
Vaneechoutte
Mario
M
D039903:Actinobacteria / Q000302:isolation & purification*; D000328:Adult; D001644:Bifidobacterium / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000302:isolation & purification; D005260:Female; D045182:Fusobacteria / Q000302:isolation & purification; D006801:Humans; D045183:Leptotrichia / Q000302:isolation & purification; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000382:microbiology*
D004275:DNA, Ribosomal