title: Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells.
abstract: The epidermal growth factor (EGF)-like domain is involved in receptor-ligand interactions, extracellular matrix formation, cell adhesion and chemotaxis. Nasopharyngeal carcinoma associated gene 6 (NGX6) is a novel EGF-like domain-containing gene located at the high frequent loss of heterozygosity (LOH) region 9p21-22 associated with nasopharyngeal carcinoma (NPC). It is down-regulated in NPC and its over-expression can delay the cell cycle G(0)-G(1) progression in NPC cells. In the present study, in situ hybridization analysis, using NPC tissue microarrays, showed that loss of NGX6 expression was associated with NPC lymph node metastasis. The Tet-on gene expression system and cDNA array techniques were used to profile the potential targets of NGX6. We found that NGX6 can influence the expression of some cell adhesion molecules in NPC cells. NGX6 can associate with ezrin, a linkage between the cell membrane and cytoskeleton. The NGX6 protein was expressed on the cell surface as a glycoprotein. Ectopic induction of NGX6 can impair NPC cell migration and invasive ability as well as improve cell adhesion and gap junctional intercellular communication, and can suppress tumor formation in vivo. The data revealed that NGX6 plays a role in cell adhesion modulation in NPC cells.
authors.affiliation: Cancer Research Institute, Xiang-Ya School of Medicine, 110 Xiang-Ya Road, Changsha, Hunan 410078, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Songqing
authors.initials: S
authors.lastname: Fan
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.forename: Qun
authors.initials: Q
authors.lastname: Yan
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Huaying
authors.initials: H
authors.lastname: Liu
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Houde
authors.initials: H
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Gan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.forename: Shourong
authors.initials: S
authors.lastname: Shen
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D019556:COS Cells; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D010750:Phosphoproteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D000099149:Ezrin
chemical_list: D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D004815:Epidermal Growth Factor; D008565:Membrane Proteins; D010750:Phosphoproteins; D025521:Tumor Suppressor Proteins; D000099149:Ezrin; C477325:TMEM8B protein, human