title: Rotavirus genotyping: keeping up with an evolving population of human rotaviruses.
abstract: The use of molecular methods for rotavirus characterisation provides not only increased sensitivity for typing, but also allows accurate and more complete characterisation of strains, and the identification of putative reassortant strains. However, due to the constant accumulation of point mutations through genetic drift, and to the emergence of novel genotypes, possibly zoonotic transmission and subsequent reassortment, the reagents and methods used require close monitoring and updating. Methods and oligonucleotide primers are described to overcome failures to type G9, G10 and P[11] rotavirus strains, and cross-reactivity identified between G10 and G3 rotaviruses.
authors.affiliation: Enteric Virus Unit, Enteric Respiratory and Neurological Virus Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK. miren.iturriza@hpa.org.uk
authors.forename: Miren
authors.initials: M
authors.lastname: Iturriza-Gómara
authors.forename: Gagandeep
authors.initials: G
authors.lastname: Kang
authors.forename: Jim
authors.initials: J
authors.lastname: Gray
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers