Molecular analysis of mutations in the human HPRT gene.
The HPRT assay uses incorporation of toxic nucleotide analogs to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosporibosyltransferase. A major advantage of this assay is the ability to isolate mutant cells and determine the molecular basis for their functional deficiency. Many types of analyses have been performed at this locus: the current protocol involves generation of a cDNA and multiplex PCR of each exon, including the intron/exon junctions, followed by direct sequencing of the products. This analysis detects point mutations, small deletions and insertions within the gene, mutations affecting RNA splicing, and the products of illegitimate V(D)J recombination within the gene. Establishment of and comparisons with mutational spectra hold the promise of identifying exposures to mutation-inducing genotoxicants from their distinctive pattern of gene-specific DNA damage at this easily analyzed reporter gene.
Keohavong
Phouthone
P
Department of Environmental and Occupational Health, University of Pittsburgh, PA, USA.
Xi
Liqiang
L
Grant
Stephen G
SG
D004252:DNA Mutational Analysis / Q000379:methods*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009152:Mutagenicity Tests; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism
D009711:Nucleotides; D007041:Hypoxanthine Phosphoribosyltransferase