Polyclonal antibody effects on the human cardiac 5-HT4(e) receptors depend upon the expression system.
The initial objective of this work was to examine the effects of an antibody (Anti-G21V) directed against the second extracellular loop of human heart 5-HT4 receptors expressed in Chinese hamster ovary (CHO) cells. The antibody anti-G21V had no effect upon either basal cAMP-or 5-HT-evoked increases in cAMP in CHO cells, whereas it had shown an agonist-like effect in COS-7 cells. Analysis of agonist fractions of h5-HT4(e) receptors in CHO and COS-7 cells revealed that equilibrium constant could underlie the different responses of the receptor toward the anti-G21V antibody. Therefore, different expression systems could give rise to functional differences in 5-HT4 receptor behavior.
Di Scala
Emmanuella
E
UMR CNRS 6542, Physiologie des Cellules Cardiaques et Vasculaires, Faculté des Sciences et Techniques, Université de, Tours, France.
Rose
Stéphanie
S
Hérault
Olivier
O
Argibay
Jorge
J
Cosnay
Pierre
P
Bozon
Véronique
V
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D006801:Humans; D009206:Myocardium / Q000276:immunology*; D044422:Receptors, Serotonin, 5-HT4 / Q000235:genetics / Q000276:immunology*; D013997:Time Factors
D000906:Antibodies; D044422:Receptors, Serotonin, 5-HT4