Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray.
The complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. To understand these molecular mechanisms, we performed cDNA microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. Microarray analysis of 4,304 cDNA clones revealed that the hybridization signal of 396 cDNA clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of leaves. Cluster analysis with the expression data showed that 259 cDNA clones (156 non redundant groups) were specifically or predominantly expressed in anther tissues and were regulated by developmental stage-specific manners in the anther tissues. These co-regulated genes would be important for development of functional anther tissues. Furthermore, we selected several clones for RNA in situ hybridization analysis. From these analyses, we found several novel genes that show temporal and spatial expression patterns during anther development in addition to anther-specific genes reported so far. These results indicate that the genes identified in this experiment are controlled by different programs and are specialized in their developmental and cell types.
Endo
Makoto
M
Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
Tsuchiya
Tohru
T
Saito
Hiroshi
H
Matsubara
Hitoshi
H
Hakozaki
Hirokazu
H
Masuko
Hiromi
H
Kamada
Motoshi
M
Higashitani
Atsushi
A
Takahashi
Hideyuki
H
Fukuda
Hiroo
H
Demura
Taku
T
Watanabe
Masao
M
D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics*; D035264:Flowers / Q000378:metabolism*; D005456:Fluorescent Dyes; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*
D018076:DNA, Complementary; D005456:Fluorescent Dyes