title: Antioxidant activity of phenylpropanoid esters isolated and identified from Platycodon grandiflorum A. DC.
abstract: Platycodon grandiflorum A. DC (Campanulaceae) is used as a traditional oriental medicine and also as a food in Korea. Here we investigated its antioxidant activity, and isolated and identified its active compounds. Petroleum ether extracts from the whole root of P. grandiflorum were fractionated by silica gel column chromatography using a solvent gradient (petroleum ether:diethyl ether, v/v; 9:1-5:5). The 8:2 fraction showed a higher radical scavenging activity than the other fractions, and active compounds were purified from this fraction by reversed-phased HPLC. Two active compounds were identified as coniferyl alcohol esters of palmitic and oleic acids by FAB-MS, UV, IR and NMR spectroscopy. The antioxidant activities of these two compounds, which were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and nitric oxide radical scavenging capacity, were found to be as high as those of BHT or BHA.
authors.affiliation: Graduate School of Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-ku, Seoul 136-701, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Yoon
authors.forename: Cheong-Tae
authors.initials: CT
authors.lastname: Kim
authors.forename: Seung-Taik
authors.initials: ST
authors.lastname: Lim
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031238:Platycodon / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D004952:Esters; D016166:Free Radical Scavengers; D010936:Plant Extracts