[The study of immunofluorescence on the retinal digest preparations].
OBJECTIVE
To study the technique of immunofluorescence staining on the digested retina preparations.


METHODS
Retinas of 5 normal Wistar rats and 5 STZ diabetic Wistar rats of were used to make digested retina preparations and carry out immunofluorescence staining with the vascular cell adhesion molecule-1 (VCAM-1).


RESULTS
The retinal vessels were clearly observed; There were a lot of positive cells in the retinal preparations of the diabetic Wistar rats, while there were no positive cells in the retinal preparations of the normal rats.


CONCLUSIONS
It is feasible to carry out immunofluorescence staining on the digested retina preparations.
Lin
Jianxian
J
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Bai
Ningyan
N
Tang
Shibo
S
Zhang
Ping
P
Feng
Guanguang
G
Zhang
Wenxin
W
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
D019010:Vascular Cell Adhesion Molecule-1