Enzyme activities and alcohol tolerance in isofemale lines of Drosophila melanogaster originating from different habitats.
Enzyme activity variation was studied in a Drosophila melanogaster population from two villages (Tiszafüred and Tiszaszolos) in Hungary. Two habitats (distillery and farmyard) were sampled in both villages and 8-9 isofemale lines were established from each sample with a total of 35 lines. The activities of ADH, alphaGPDH, IDH and 6PGDH were determined on starch gel after electrophoresis in 10 F1 females of each of the 35 isofemale lines. Three sublines were established from three selected isofemale lines of all four samples (altogether 36 sublines). Alcohol tolerance of the adult flies was assayed in these sublines. The activity of ADH was similar in the two habitats; so was the sensitivity to ethanol. Accordingly, no differences in adaptation to environmental ethanol were detected between the two habitats. The deviations between the two habitats in average activities and in the total variation of enzyme activities were not consistent in the two villages. These results suggest that founder effects and genetic drift are more pronounced in distilleries than selection. The association among enzyme activities varied greatly both between the two villages and between the two habitats. The two parameters of alcohol tolerance were not significantly different between the two habitats in any of the two villages.
Pecsenye
Katalin
K
Department of Evolutionary Zoology and Human Biology, University of Debrecen, Debrecen, Egyetem tér 1., H-4010 Hungary. pecskati@tigris.klte.hu
Saura
Anssi
A
D000426:Alcohol Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D003433:Crosses, Genetic; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D004592:Electrophoresis, Starch Gel; D004777:Environment; D000431:Ethanol / Q000378:metabolism*; D005260:Female; D018703:Founder Effect; D040961:Genetic Drift; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*
D029721:Drosophila Proteins; D000431:Ethanol; D000429:Alcohol Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; C073796:ADH protein, Drosophila; D000426:Alcohol Dehydrogenase; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase