The relation of Helicobacter pylori with intestinal metaplasia, gastric atrophy and BCL-2.
OBJECTIVE
It is assumed that the sequence of events in gastric cancer is as follows: chronic gastritis, atrophy, intestinal metaplasia (IM), dysplasia, and carcinoma. It is also known that Helicobacter pylori (Hp) can be involved in the chain of these chronic phenomena. Therefore, we studied the relation of Hp with chronic inflammation, atrophy, activity level, IM and bcl-2.


METHODS
The number of patients included in this study was 52; 35 (67.3%) were female and 17 (32.7%) were male. The average ages of women and men were determined as 44.5 and 41.5 years, respectively. Hp was determined in 53.8% of all cases and chronic inflammation was found in all cases. No dysplasia or gastric cancer was found in our cases. A statistically significant positive correlation was found between the Hp intensity and the degrees of chronic inflammation, atrophy and activity.


RESULTS
Bcl-2 was found positive in 7.1% of the patients with Hp, and in 4.1% of the Hp-negative patients. However, bcl-2 was positive in 2 (8.7%) of 23 Hp-positive patients with chronic atrophic gastritis and in 1 (11.1%) of 9 Hp-negative patients with chronic atrophic gastritis. In other words, bcl-2 was found more in Hp-negative chronic atrophic gastritis than in Hp-positive chronic atrophic gastritis. Also, atrophy and IM were present together in 2 bcl-2-positive patients in whom Hp was positive. Bcl-2 was found positive more in IM than atrophy, proportionally. According to these data, although bcl-2 was found positive more often in Hp (+) cases proportionally, it was not statistically significant. Nevertheless, no significant difference was found between bcl-2 positivity and atrophy according to the statistical data. The data regarding the relation between IM and bcl-2 were statistically significant, and a positive correlation was found between them.


CONCLUSIONS
We concluded that Hp infections result in chronic gastritis, have a role in the development of atrophy and IM, and that Hp infection has a significant relation with neutrophile activation. The more Hp intensity increases, the greater the degrees of chronic gastritis, activity and atrophy. We also found that Hp, which is known to increase apoptosis, increases the expression of bcl-2, an anti-apoptotic gene, not directly but rather by causing atrophy development. We observed more bcl-2 positivity in IM than in atrophy. Further studies with an extensive series of cases, including patients with dysplasia and gastric cancer, are needed to clarify statistical rates and to support the suggestion that bcl-2 expression increases with the progression to gastric cancer.
Topal
Derya
D
Department of Gastroenterology, Dicle University School of Medicine, Diyarbakir, Turkey.
Göral
Vedat
V
Yilmaz
Fahri
F
Kara
Ismail Hamdi
IH
D000328:Adult; D001706:Biopsy; D005260:Female; D005757:Gastritis, Atrophic / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D016481:Helicobacter Infections / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D007249:Inflammation; D007422:Intestines / Q000382:microbiology / Q000473:pathology*; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D011446:Prospective Studies; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012720:Severity of Illness Index; D013270:Stomach / Q000382:microbiology / Q000473:pathology
D019253:Proto-Oncogene Proteins c-bcl-2