title: Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus.
abstract: The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target.
authors.affiliation: Centro de Biología Molecular Severo Ochoa CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Renata
authors.initials: R
authors.lastname: Moreno
authors.forename: Aurelio
authors.initials: A
authors.lastname: Hidalgo
authors.forename: Felipe
authors.initials: F
authors.lastname: Cava
authors.forename: Roberto
authors.initials: R
authors.lastname: Fernández-Lafuente
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Guisán
authors.forename: José
authors.initials: J
authors.lastname: Berenguer
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007613:Kanamycin Resistance / Q000235:genetics; D008345:Manganese / Q000378:metabolism*; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger; D008345:Manganese; D002374:Catalase