C-kit receptor and its possible function in human spermatozoa.
The presence and role of the c-kit proto-oncogene protein was investigated in the mature sperm of the human. A polyclonal antibody against the c-kit peptide was used to perform immunohistochemical (IHC) staining, electron microscopy (EM) studies, and Western blot analysis. The acrosomal region of fresh sperm specifically stained with the antibody. No acrosomal staining or staining limited to the equatorial region was noted in the acrosome-reacted (AR) sperm. EM studies demonstrated immunogold label on the plasma membrane (PM) of the acrosome, and confirmed the lack of binding following the acrosome reaction. A 150 kDa band was detected by Western blot analysis. This protein was released from the sperm surface during sperm capacitation and the acrosome reaction. Antibody against the c-kit receptor significantly inhibited the acrosome reaction and increased sperm agglutination, but did not significantly inhibit sperm motility. These results suggest that the c-kit receptor protein is present in mature human sperm and is released during capacitation and/or the acrosome reaction. The assessment of the c-kit receptor may also be a useful assay for sperm function in male infertility.
Feng
Huai L
HL
Center for Human Reproduction, North Shore University Hospital, NYU School of Medicine, Manhasset, New York, USA.
Sandlow
J I
JI
Zheng
L J
LJ
D000177:Acrosome / Q000276:immunology / Q000502:physiology; D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology*; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D002462:Cell Membrane / Q000276:immunology / Q000502:physiology; D006801:Humans; D008297:Male; D000090063:Proto-Oncogene Mas; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D013073:Sperm Agglutination / Q000187:drug effects / Q000502:physiology; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure
D000906:Antibodies; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D019009:Proto-Oncogene Proteins c-kit