title: Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells.
abstract: In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extracellular matrix production via redox-sensitive signaling pathways. We investigated the role(s) of ROS in proliferation, migration and collagen synthesis induced by BK in VSMC derived from Sprague Dawley rat aorta. BK (10 nM) increased VSMC proliferation by 30% (n=5); this proliferation was inhibited by the antioxidants N-acetylcysteine (20 mM) and alpha-lipoic acid (LA, 250 mM). In addition, BK induced an increase in cell migration and in collagen levels that were blocked by LA. ROS production induced by BK (n=10) was significantly inhibited by bisindolylmaleimide (4microM) and by PD98059 (40microM). These results suggest that: 1) ROS participate in the mechanism(s) used by bradykinin to induce cellular proliferation; 2) bradykinin induces ROS generation through a pathway that involves the kinases PKC and MEK; and 3) ROS participate in the pathways mediating cell migration and the production of collagen as a response to treatment with bradykinin. To our knowledge, this is the first report describing mechanisms to explain the participation of ROS in the cellular proliferation and extracellular matrix pathway regulated by BK.
authors.affiliation: Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. mvelarde@bio.puc.cl
authors.forename: Victoria
authors.initials: V
authors.lastname: Velarde
authors.forename: Paula M
authors.initials: PM
authors.lastname: de la Cerda
authors.forename: Claudia
authors.initials: C
authors.lastname: Duarte
authors.forename: Francisca
authors.initials: F
authors.lastname: Arancibia
authors.forename: Eduardo
authors.initials: E
authors.lastname: Abbott
authors.forename: Alejandro
authors.initials: A
authors.lastname: González
authors.forename: Francesca
authors.initials: F
authors.lastname: Moreno
authors.forename: Ayad A
authors.initials: AA
authors.lastname: Jaffa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology; D001920:Bradykinin / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D003094:Collagen; D001920:Bradykinin