title: Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal rieske [2Fe-2S] center.
abstract: The weakly coupled 15N atoms around a reduced Rieske [2Fe-2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3-0.4 MHz for the Nepsilon and 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Ndelta atoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron-proton transfer reactions.
authors.affiliation: Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. tiwasaki@nms.ac.jp
authors.forename: Toshio
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Asako
authors.initials: A
authors.lastname: Kounosu
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Uzawa
authors.forename: Rimma I
authors.initials: RI
authors.lastname: Samoilova
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Dikanov
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000378:metabolism; D009587:Nitrogen Isotopes; D016944:Sulfolobus / Q000737:chemistry / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D007506:Iron-Sulfur Proteins; D009587:Nitrogen Isotopes; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III