title: Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle.
abstract: To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.
authors.affiliation: Department of Cell Biology ICND202, the Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: Karine G
authors.initials: KG
authors.lastname: Le Roch
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Yingyao
authors.initials: Y
authors.lastname: Zhou
authors.forename: Andrey
authors.initials: A
authors.lastname: Santrosyan
authors.forename: Munira
authors.initials: M
authors.lastname: Grainger
authors.forename: S Frank
authors.initials: SF
authors.lastname: Yan
authors.forename: Kim C
authors.initials: KC
authors.lastname: Williamson
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Holder
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Carucci
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Winzeler
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics; D017125:Genes, Protozoan; D008018:Life Cycle Stages / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016053:RNA, Protozoan / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan