Chromosome analysis of mouse zygotes after injecting oocytes with spermatozoa treated in vitro with green tea catechin, (-)-epigallocatechin gallate (EGCG).
The cytogenetic effects of (-)-epigallocatechin gallate (EGCG) on mouse spermatozoa were studied in vitro using an intracytoplasmic sperm injection (ICSI) technique. Spermatozoa were collected by the swim-up method and treated with EGCG at 1 microM and 10 microM. When motile, EGCG-treated spermatozoa were injected into oocytes, structural chromosome aberrations (SCAs) at the first cleavage metaphase did not increase significantly. However, a majority of immotile spermatozoa treated with 10 microM EGCG had the following abnormalities: pronuclear arrest (11% of activated oocytes), degenerated sperm chromatin (chromosome) mass (30% of activated oocytes) and occurrence of structural chromosome aberrations (57% of analyzed metaphases). The incidence of these abnormalities suggests that immotile spermatozoa were susceptible to EGCG, and that the damage of sperm chromatin was accelerated in immotile spermatozoa by 10 microM EGCG treatment.
Kusakabe
Hirokazu
H
Department of Biological Sciences, Asahikawa Medical College, Asahikawa 078-8510, Japan. hkusa55@asahikawa-med.ac.jp
Kamiguchi
Yujiroh
Y
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000633:toxicity*; D002843:Chromatin / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects*; D020732:Cytogenetic Analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D009865:Oocytes / Q000187:drug effects; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000187:drug effects*; D015053:Zygote / Q000187:drug effects*
D002843:Chromatin; D002392:Catechin; C045651:epigallocatechin gallate