title: Mammary cell turnover in lactating ewes is modulated by changes of energy fuels.
abstract: The study of cell turnover of mammary gland helps to gain understanding of the subtle modulation that largely determines milk production. To evaluate the effect of diet composition on cell turnover of mammary gland, pluriparous sheep were allotted to three experimental groups and fed from day 33 after lambing a control diet (BD), a high starch (HS) diet or a high fat (HF) diet. Biopsies of mammary gland tissue were collected at 50 days after lambing for in situ detection of cell death and RT-PCR analysis of bax, bcl-2, caspase-3 and GST expressions. Both apoptotic and proliferation indexes were significantly higher (P<0.05) in the BD group compared to HS and HF groups. The relative expressions of GST were significantly greater (P<0.05) in HF group compared to the BD and HS groups. There was a significant increase in the ratio of bcl-2 to bax mRNA in the HS and HF groups. Availability of energy substrates for mammary gland can interfere with the cell fate, modulating genes involved in the control of oxidative stress which, in turn, can indirectly regulate cell apoptosis and proliferation.
authors.affiliation: Dipartimento di Scienze della Produzione Animale, Facoltà di Medicina Veterinaria, Università di Udine, Via delle Scienze, 208, 33100 Udine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Colitti
authors.forename: G
authors.initials: G
authors.lastname: Stradaioli
authors.forename: B
authors.initials: B
authors.lastname: Stefanon
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D007774:Lactation / Q000378:metabolism / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D012756:Sheep / Q000378:metabolism / Q000502:physiology*
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats