title: Large-scale investigation of genomic markers for severe periodontitis.
abstract: The purpose of the present study was to investigate the genomic markers for periodontitis, using large-scale single-nucleotide polymorphism (SNP) association studies comparing healthy volunteers and patients with periodontitis. Genomic DNA was obtained from 19 healthy volunteers and 22 patients with severe periodontitis, all of whom were Japanese. The subjects were genotyped at 637 SNPs in 244 genes on a large scale, using the TaqMan polymerase chain reaction (PCR) system. Statistically significant differences in allele and genotype frequencies were analyzed with Fisher's exact test. We found statistically significant differences (P < 0.01) between the healthy volunteers and patients with severe periodontitis in the following genes; gonadotropin-releasing hormone 1 (GNRH1), phosphatidylinositol 3-kinase regulatory 1 (PIK3R1), dipeptidylpeptidase 4 (DPP4), fibrinogen-like 2 (FGL2), and calcitonin receptor (CALCR). These results suggest that SNPs in the GNRH1, PIK3R1, DPP4, FGL2, and CALCR genes are genomic markers for severe periodontitis. Our findings indicate the necessity of analyzing SNPs in genes on a large scale (i.e., genome-wide approach), to identify genomic markers for periodontitis.
authors.affiliation: General Dentistry, The Nippon Dental University Hospital at Tokyo, 2-3-16 Fujimi, Chiyoda-ku, Tokyo, Japan. ndu-a-suzuki@nyc.odn.ne.jp.
authors.forename: Asami
authors.initials: A
authors.lastname: Suzuki
authors.forename: Guijin
authors.initials: G
authors.lastname: Ji
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Numabe
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ishii
authors.forename: Masaaki
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Kyuichi
authors.initials: K
authors.lastname: Kamoi
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics; D000328:Adult; D000483:Alleles; D004247:DNA / Q000235:genetics; D018819:Dipeptidyl Peptidase 4; D005260:Female; D005340:Fibrinogen / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D015894:Genome, Human; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D007564:Japan; D008297:Male; D010518:Periodontitis / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018003:Receptors, Calcitonin / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: C095202:FGL2 protein, human; D005819:Genetic Markers; D006023:Glycoproteins; D007527:Isoenzymes; D018003:Receptors, Calcitonin; D007987:Gonadotropin-Releasing Hormone; D005340:Fibrinogen; D004247:DNA; D019869:Phosphatidylinositol 3-Kinases; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D000243:Adenosine Deaminase