Improved functional outcome after spinal cord injury in iNOS-deficient mice.
METHODS
Functional outcome was evaluated following experimental compression-type spinal cord injury (SCI) in wild-type mice and knockout mice, lacking the inducible nitric oxide synthase (iNOS) gene.


OBJECTIVE
To evaluate the role of the nitric oxide generating enzyme iNOS in SCI.


METHODS
The experimental animals were subjected to an extradural compression of the thoracic spinal cord. Functional outcome was studied during the first 2 weeks post-injury using a scoring system for assessment of hind limb motor function.


RESULTS
Injury resulted in initial paraplegia followed by gradual improvement of motor function in most cases. Mice lacking the iNOS gene (iNOS-/-) clearly tended to have a better functional outcome than wild-type mice. The difference was significant on day 14 after injury.


CONCLUSIONS
In accordance with a few earlier experimental studies, showing beneficial effects of pharmacological iNOS inhibition, the present report would indicate a destructive influence of iNOS following spinal cord trauma.
Isaksson
J
J
Research Group of Neuropathology, Department of Genetics and Pathology, Uppsala University Hospital, Sweden.
Farooque
M
M
Olsson
Y
Y
D000818:Animals; D005260:Female; D020233:Gait Disorders, Neurologic / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology*; D006614:Hindlimb / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019001:Nitric Oxide Synthase / Q000172:deficiency*; D052247:Nitric Oxide Synthase Type II; D020127:Recovery of Function / Q000502:physiology*; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology*; D013117:Spinal Cord Compression / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology*
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse