title: Effects of sperm concentrations and culture media on fertilization and development of in vitro matured pig oocytes.
abstract: This study was carried out to investigate the effects of sperm concentrations and culture media on fertilization and development of in vitro matured pig oocytes. The concentrations of frozen-thawed sperm were 0.2 x 10(7), 2 x 10(7), 20 x 10(7) and 200 x 10(7)/ml, respectively. Culture media were NCSU-23, HEPES-buffered (25 mM) NCSU-23, PZM-3 and PZM-4, respectively. Increasing the sperm concentration from 0.2 x 10(7) to 2 x 10(7)/ml, significantly increased the penetration rate. Also, increasing the sperm concentration from 20 x 10(7) to 200 x 10(7)/ml increased the penetration rate from 62.1% to 69.9%, respectively, with no differences between these two concentrations. A similar pattern was observed for polyspermic penetration and male pronucleus formation. The mean number of sperm per oocyte significantly increased in the 20 x 10(7)/ml and again in the 200 x 10(7)/ml sperm concentrations. The percentage of blastocysts from cleaved oocytes at the 2 x 10(7)/ml sperm concentration was significantly higher than that at the 0.2 x 10(7), 20 x 10(7) and 200 x 10(7)/ml sperm concentrations. The percentage of blastocysts from cleaved oocytes and the cell numbers per blastocyst were significantly higher in the HEPES-buffered NCSU-23 culture medium than in the NCSU-23, PZM-3 and PZM-4 culture media under a gas atmosphere of 5% CO2 in air.
authors.affiliation: Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University, 305-764 Daejeon, Republic of Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Yi
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013552:Swine
chemical_list: D003470:Culture Media