[Methods of gene therapy for inner ear diseases].
OBJECTIVE
To study an effective, safety and convenient method for gene transfer and introduction to inner ear.


METHODS
Ad GFP were transferred into inner ears of 30 guinea pigs through round window, basal turn of cochlea and cerebrospinal fluid respectively. Auditory brainstem response (ABR)and otoacoustic emission of distortion products (DPOAE) were assayed to these animals preoperatively and postoperatively, then transfection and expression of Ad-GFP in inner ears and structure of cochlear hair cells were observed.


RESULTS
Transfection and expression of Ad-GFP were found in spiral ganglion of cochlea, stria vascularis and Cortisorgan through all of the three methods. The expression products were in the highest level at the third day after operation. But the operation through round window and basal turn of cochlea could lead to bleeding and coagulum deposited in parietalgyrus, which could result in hair cells injury. Changes of ABR threshold of these animals were not significant. But amplitude of DPOAE decreased significantly in animals through round window and basal turn of cochlea pathways. There was no hair cells injury and changes of DPOAE through cerebrospinal fluid pathway.


CONCLUSIONS
The study suggested the operations through round window and basal turn of cochlea could lead to bleeding in cochleas, which resulted in change of DPOAE amplitude. It is a safe and effective method to transfer through cerebrospinal fluid.
Li
Shengli
S
Department of Hearing and Acoustics, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004.
Zhu
Hongliang
H
Wang
Xiaoxia
X
Liu
Hui
H
Huang
Yichuan
Y
Yao
Xiaobao
X
D000818:Animals; D007758:Ear, Inner; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006168:Guinea Pigs; D007759:Labyrinth Diseases / Q000503:physiopathology / Q000628:therapy*; D008297:Male