title: Long-term follow-up study of segmental and focal vitiligo treated by autologous, noncultured melanocyte-keratinocyte cell transplantation.
abstract: OBJECTIVE
  To evaluate long-term efficacy and safety of melanocyte-keratinocyte cell transplantation in the management of segmental and focal vitiligo.
  
  
  METHODS
  A simpler and modified method based on that of Olsson and Juhlin was performed. This method uses a shaved biopsy skin sample up to one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the dermabraded depigmented skin area, and a collagen dressing is applied to keep it in place.
  
  
  METHODS
  Fifty patients with segmental and 17 with focal vitiligo were treated. One patient with segmental and 2 with focal vitiligo did not attend any follow-up visits. The remaining patients were observed for a period of up to 5 years.
  
  
  METHODS
  Autologous, noncultured melanocyte-keratinocyte cell transplantation.
  
  
  METHODS
  Repigmentation was graded as excellent with 95% to 100% pigmentation, good with 65% to 94%, fair with 25% to 64%, and poor with 0% to 24% of the treated area.
  
  
  RESULTS
  In the segmental vitiligo group, 41 patients (84%) showed excellent, 3 (6%) good, and 5 (10%) poor pigmentation, which was retained until the end of the respective follow-up period. In the focal vitiligo group, 11 patients (73%) showed excellent, 1 (7%) fair, and 3 (20%) poor pigmentation, which was retained until the end of the respective follow-up period.
  
  
  CONCLUSIONS
  Melanocyte-keratinocyte cell transplantation is a simple, safe, and effective surgical therapy. Patients with segmental and focal vitiligo can experience a prolonged disease-free period, which may extend through the rest of their lives.
authors.affiliation: Noble Clinic, Pune, India. dr_mulekar@vsnl.com
authors.forename: Sanjeev V
authors.initials: SV
authors.lastname: Mulekar
mesh_terms: D000328:Adult; D002478:Cells, Cultured / Q000637:transplantation; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D053685:Laser Therapy; D008137:Longitudinal Studies; D008297:Male; D008544:Melanocytes / Q000637:transplantation*; D012880:Skin Pigmentation; D013535:Suspensions; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014820:Vitiligo / Q000473:pathology / Q000601:surgery*
chemical_list: D013535:Suspensions