title: Substrate profile variation and drug resistance patterns of â - lactamase producing Shigella species isolated from diarrhoeal patients in Lagos, Nigeria.
abstract: The number and trend of antibiotic resistance by Shigella species recovered from food and diarrhoeal stools are on the increase in Nigeria and has resulted in a high frequency of hospitalisation. Increased cost of disease management, and higher mortality in children. This study exposes 51 â-lactamase producing Shigella isolates from Lagos to some newly introduced drugs in the country. The drugs include â-lactam - â-actamase inhibitor antibiotics. â-lctam substrate hydrolysis and inhibitory effects of clavulanate were also investigated in-vitro. Results obtained revealed that all the isolates showed high level resistance to tetracycline, ampicillin, streptomycin, co-trimoxazole and amoxicillin with an MIC range of 128 - 1024 microg/ml. The isolates were susceptible to piperacillin, tobramycin, aztreonam and ofloxacin (0.03 - 8 microg/ ml). 18.2 - 40.9% of S. flexneri and S. dysenteriae showed low level resistance to cefuroxime and cefotaxime (MIC = 4 - 16 microg/ ml). Among the â-lactam - â- lctamase inhibitors tested, only piperacillin-tazobactam showed 100% resistance. Hydrolysis of â -lctam substrate was found to be species dependent in decreasing order of S. flexneri, S. dysenteriae, S. sonnei and S.boydii. An IC50 range of 0.8-2.4 mM was also observed in these isolates. Our data indicate that the incidence of multidrug resistance is high among â-lactamase producing Shigella isolates in Lagos, Nigeria. While the third generation cephalosporins should be used with cautions, some of the newly introduced drugs have the prospects of being used in the future control and management of shigellosis in the country.
authors.affiliation: Derpartment of Biochemistry, College of Medicine, University of Lagos, Nigeria. bamwal@yahoo.com
authors.forename: B A
authors.initials: BA
authors.lastname: Iwalokur
authors.forename: G O
authors.initials: GO
authors.lastname: Gbenle
authors.forename: E O
authors.initials: EO
authors.lastname: Akinrinmisi
authors.forename: S I
authors.initials: SI
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Ogunledun
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003967:Diarrhea / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D004405:Dysentery, Bacillary / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D012760:Shigella / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases