Anti-complementary activity of triterpenoides from fruits of Zizyphus jujuba.
In order to determine on the anti-complement activity of triterpenes, following eleven triterpenoides were isolated from the fruits of the Zizyphus jujuba MILL: ceanothane-type triterpenes: colubrinic acid (1), zizyberenalic acid (11); lupane-type triterpenes: alphitolic acid (2), 3-O-cis-p-coumaroyl alphitolic acid (3), 3-O-trans-p-coumaroyl alphitolic acid (4), betulinic acid (7), betulonic acid (9); and oleanane-type triterpenes: 3-O-cis-p-coumaroyl maslinic acid (5), 3-O-trans-p-coumaroyl maslinic acid (6), oleanolic acid (8), oleanonic acid (10). These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Among them, compounds 5, 6, and 8 exhibited significant anti-complement activity with IC(50) values of 101.4, 143.9, and 163.4 microM, respectively, whereas the ceanothane-type and the lupane-type triterpenes were inactive. This suggests that the oleanane-structure plays an important role in inhibiting the hemolytic activity of human serum against erythrocytes.
Lee
Sang-Myung
SM
KT&G Central Research Institute, Yuseong, Daejeon, Korea.
Park
Jin-Gyu
JG
Lee
You-Hee
YH
Lee
Cheal-Gyu
CG
Min
Byung-Sun
BS
Kim
Jung-Hee
JH
Lee
Hyeong-Kyu
HK
D002851:Chromatography, High Pressure Liquid; D003169:Complement Inactivator Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects; D005638:Fruit; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031957:Ziziphus / Q000737:chemistry*
D003169:Complement Inactivator Proteins; D014315:Triterpenes