title: A method to extract algae toxin of microcystin-LR.
abstract: A simple and low-cost method to obtain cyanobacterial toxin microcystin-LR (MC-LR) was developed. A new strain of Microcystis aeruginosa, named DC-1, producing microcystin-LR but not microcystin-RR, was separated from the field blooming algae samples of Dianchi Lake, in southwest of China. Following three times' freeze and thaw treatment, the cultivated DC-1 cells were extracted with 40% methanol in water. The extract was centrifuged and the supernatant applied to a Hydrophilic-Lipophilic Balance (HLB) SPE cartridge. Eluted impurities with a certain gradient from 30% to 50% methanol in water, MC-LR was finally eluted from the HLB cartridge with 60% methanol in water, and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR samples may be used in adsorption and biodegradation experiments instead of using expensive standard reagents.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Pan
authors.forename: Hai
authors.initials: H
authors.lastname: Yan
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D000458:Cyanobacteria; D004791:Enzyme Inhibitors / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors
chemical_list: D001427:Bacterial Toxins; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D010749:Phosphoprotein Phosphatases; C057862:cyanoginosin LR