title: The effect of processing and cryopreservation on nucleated umbilical cord blood cells.
abstract: Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque or Puregene red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 +/- 10% (average +/- standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 +/- 20% compared to 91+/-11% (p<0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen.
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth's Medical Center, Boston, MA 02135, U.S.A. zwirello@massmed.org
authors.forename: Lucy A
authors.initials: LA
authors.lastname: Bayer-Zwirello
authors.forename: Despina E
authors.initials: DE
authors.lastname: Hoffman
authors.forename: Lorrie A
authors.initials: LA
authors.lastname: Adams
authors.forename: Paul T
authors.initials: PT
authors.lastname: Wilder
authors.forename: Margaret T
authors.initials: MT
authors.lastname: Reece
mesh_terms: D018952:Antigens, CD34; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D011247:Pregnancy
chemical_list: D018952:Antigens, CD34; D003451:Cryoprotective Agents; D017493:Leukocyte Common Antigens; D004121:Dimethyl Sulfoxide