title: Comparative culturing of Pleurotus spp. on coffee pulp and wheat straw: biomass production and substrate biodegradation.
abstract: The results of the cultivation of six strains of Pleurotus (P. djamor (2), P. ostreatus (2) and P. pulmonarius (2)) on coffee pulp and wheat straw are presented. Metabolic activity associated with biomass of each strain was determined, as well as changes in lignin and polysaccharides (cellulose and hemicellulose), phenolic and caffeine contents in substrate samples colonized for a period of up to 36 days. Analysis were made of changes during the mycelium incubation period (16 days) and throughout different stages of fructification. Greater metabolic activity was observed in the wheat straw samples, with a significant increase between 4 and 12 days of incubation. The degradation of polysaccharide compounds was associated with the fruiting stage, while the reduction in phenolic contents was detected in both substrates samples during the first eight days of incubation. A decrease was observed in caffeine content of the coffee pulp samples during fruiting stage, which could mean that some caffeine accumulates in the fruiting bodies.
authors.affiliation: Laboratorio de Hongos Comestibles, Departamento de Hongos, Instituto de Ecología, P.O. Box 63, 91070 Xalapa, Mexico. dulce@ecologia.edu.mx
authors.forename: Dulce
authors.initials: D
authors.lastname: Salmones
authors.forename: Gerardo
authors.initials: G
authors.lastname: Mata
authors.forename: Krzysztof N
authors.initials: KN
authors.lastname: Waliszewski
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D002110:Caffeine / Q000378:metabolism; D003069:Coffee / Q000378:metabolism; D003470:Culture Media / Q000032:analysis*; D005452:Fluoresceins / Q000378:metabolism; D020076:Pleurotus / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism; D014866:Waste Products / Q000032:analysis
chemical_list: D003069:Coffee; D003470:Culture Media; D005452:Fluoresceins; D011134:Polysaccharides; D014866:Waste Products; D002110:Caffeine; C018506:diacetylfluorescein