Genome-scale models of microbial cells: evaluating the consequences of constraints.
Microbial cells operate under governing constraints that limit their range of possible functions. With the availability of annotated genome sequences, it has become possible to reconstruct genome-scale biochemical reaction networks for microorganisms. The imposition of governing constraints on a reconstructed biochemical network leads to the definition of achievable cellular functions. In recent years, a substantial and growing toolbox of computational analysis methods has been developed to study the characteristics and capabilities of microorganisms using a constraint-based reconstruction and analysis (COBRA) approach. This approach provides a biochemically and genetically consistent framework for the generation of hypotheses and the testing of functions of microbial cells.
Price
Nathan D
ND
Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.
Reed
Jennifer L
JL
Palsson
Bernhard Ø
BØ
D018407:Bacterial Physiological Phenomena; D019295:Computational Biology; D016680:Genome, Bacterial; D008433:Mathematics; D008954:Models, Biological; D010641:Phenotype; D012984:Software; D013598:Systems Theory