Mismatch repair ensures fidelity of replication and recombination in the radioresistant organism Deinococcus radiodurans.
We have characterized the mismatch repair system (MMR) of the highly radiation-resistant type strain of Deinococcus radiodurans, ATCC 13939. We show that the MMR system is functional in this organism, where it participates in ensuring the fidelity of DNA replication and recombination. The system relies on the activity of two key proteins, MutS1 and MutL, which constitute a conserved core involved in mismatch recognition. Inactivation of MutS1 or MutL resulted in a seven-fold increase in the frequency of spontaneous RifR mutagenesis and a ten-fold increase in the efficiency of integration of a donor point-mutation marker during bacterial transformation. Inactivation of the mismatch repair-associated UvrD helicase increased the level of spontaneous mutagenesis, but had no effect on marker integration--suggesting that binding of MutS1 and MutL proteins to a mismatched heteroduplex suffices to inhibit recombination between non identical (homeologous) DNAs. In contrast, inactivation of MutS2, encoded by the second mutS -related gene present in D. radiodurans, had no effect on mutagenesis or recombination. Cells devoid of MutS1 or MutL proteins were as resistant to gamma-rays, mitomycin C and UV-irradiation as wild-type bacteria, suggesting that the mismatch repair system is not essential for the reconstitution of a functional genome after DNA damage.
Mennecier
S
S
Institut de Génétique et Microbiologie, Bâtiment 409, Université Paris-Sud, 91405 Orsay Cedex, France.
Coste
G
G
Servant
P
P
Bailone
A
A
Sommer
S
S
D000595:Amino Acid Sequence; D020137:Base Pair Mismatch / Q000235:genetics*; D001483:Base Sequence; D004260:DNA Repair / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D034301:Deinococcus / Q000235:genetics* / Q000528:radiation effects; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005720:Gamma Rays; D016685:Mitomycin; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D011755:Pyrophosphatases; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D029968:Escherichia coli Proteins; D009692:Nucleic Acid Heteroduplexes; D016685:Mitomycin; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; C052211:mutT protein, E coli