Initiation of DNA replication at a nuclear matrix-attached chromatin fraction.
It is still unclear what nuclear components support initiation of DNA replication. To address this issue, we developed a cell-free replication system in which the nuclear matrix along with the residual matrix-attached chromatin was used as a substrate for DNA replication. We found out that initiation occurred at late G1 residual chromatin but not at early G1 chromatin and depended on cytosolic and nuclear factors present in S phase cells but not in G1 cells. Initiation of DNA replication occurred at discrete replication foci in a pattern typical for early S phase. To prove that the observed initiation takes place at legitimate DNA replication origins, the in vitro synthesized nascent DNA strands were isolated and analyzed. It was shown that they were enriched in sequences from the core origin region of the early firing, dihydrofolate reductase origin of replication ori-beta and not in distal to the origin sequences. A conclusion is drawn that initiation of DNA replication occurs at discrete sub-chromosomal structures attached to the nuclear matrix.
Radichev
Ilian
I
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Parashkevova
Anastassia
A
Anachkova
Boyka
B
D000818:Animals; D016466:CHO Cells; D002458:Cell Fractionation; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D006224:Cricetinae; D004261:DNA Replication / Q000502:physiology*; D016193:G1 Phase / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D015530:Nuclear Matrix / Q000378:metabolism / Q000502:physiology*; D018741:Replication Origin / Q000502:physiology; D016196:S Phase / Q000502:physiology
D002843:Chromatin