Absence of ras-gene hot-spot mutations in canine fibrosarcomas and melanomas.
Point mutations within ras proto-oncogenes, particularly within the mutational hot-spot codons 12, 13 and 61, are frequently detected in human malignancies and in different types of experimentally-induced tumours in animals. So far little is known about ras mutations in naturally occurring canine fibrosarcomas or K-ras mutations in canine melanomas. To elucidate whether ras mutations exist in these naturally occurring tumours in dogs, in the present study we screened 13 canine fibrosarcomas, 2 feline fibrosarcomas and 11 canine melanomas for point mutations, particularly within the mutational hot-spots, making this the first study to investigate a large number of canine fibrosarcomas. None of the samples showed a K- or N-ras hot spot mutation. Thus, our data strongly suggest that ras mutations at the hot-spot loci are very rare and do not play a major role in the pathogenesis of the spontaneously occurring canine tumours investigated.
Murua Escobar
Hugo
H
Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 Bremen, Germany.
Günther
Kathrin
K
Richter
Andreas
A
Soller
Jan T
JT
Winkler
Susanne
S
Nolte
Ingo
I
Bullerdiek
Jörn
J
D000818:Animals; D002371:Cat Diseases / Q000235:genetics; D002415:Cats; D003062:Codon; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005354:Fibrosarcoma / Q000235:genetics* / Q000662:veterinary*; D011905:Genes, ras / Q000235:genetics*; D008545:Melanoma / Q000235:genetics* / Q000662:veterinary*; D017354:Point Mutation
D003062:Codon