title: Sp1 is involved in the transcriptional activation of lysozyme in epithelial cells.
abstract: Lysozyme protects us from the ever-present danger of bacterial infection. The expression of lysozyme is, in part, regulated by the Ets factor, myeloid elf-1-like factor (MEF). MEF binds to the ETS site of the lysozyme promoter at -46 to -40bp. Closer analysis of the promoter using a series of deletion mutants and point mutants indicated that the region around -75bp is also essential in regulating the activity of lysozyme. The sequences in this region correspond to the Sp1 consensus binding site. Sp1 is known to regulate a variety of house-keeping and tissue-specific genes by itself or with other transcription factors like AP-1 or ETS. We indicate here that Sp1 regulates the lysozyme gene by binding to the GT-core sequences of lysozyme promoter. Treatment with mithramycin A down-regulated the promoter activity and the transfection of anti-sense Sp1 induced a decrease in the endogenous expression of lysozyme.
authors.affiliation: Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Suico
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Koga
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shuto
authors.forename: Akinori
authors.initials: A
authors.lastname: Hisatsune
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lu
authors.forename: Carol
authors.initials: C
authors.lastname: Basbaum
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Okiyoneda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kai
mesh_terms: D024506:5' Flanking Region; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000201:enzymology; D006801:Humans; D009113:Muramidase / Q000096:biosynthesis / Q000235:genetics*; D008926:Plicamycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D016329:Sp1 Transcription Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C066851:mithramycin A; D009113:Muramidase; D008926:Plicamycin