Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.
Mouse autocrine motility factor (mAMF), a tumour-secreted cytokine that stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 69.97, b = 115.88, c = 73.27 A, beta = 101.76 degrees . There are two subunits (one dimer) per asymmetric unit. Complexes with four-, five- and six-carbon carbohydrate phosphate inhibitors have also been crystallized. The crystals diffract to at least 1.8 A resolution and are suitable for X-ray structure analyses at high resolution.
Naba
Noriko
N
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Tanaka
Nobutada
N
Shiraiwa
Katsura
K
Kusakabe
Yoshio
Y
Funasaka
Tatsuyoshi
T
Haga
Arayo
A
Nagase
Hisamitsu
H
Raz
Avraham
A
Nakamura
Kazuo T
KT
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice
D005956:Glucose-6-Phosphate Isomerase