title: New strategy for the synthesis of 3',5'-bifunctionalized oligonucleotide conjugates through sequential formation of chemoselective oxime bonds.
abstract: A convenient strategy for the synthesis of bifunctionalized oligonucleotide conjugates bearing two different reporters at the 3' and 5' ends of the oligonucleotide is presented. The method involves the preparation of oligonucleotides bearing an aldehyde and/or aminooxy functionality at each end, followed by reaction to form oxime bonds with appropriately functionalized reporters. The conjugation reactions are carried out under mild aqueous conditions with good reaction yield.
authors.affiliation: LEDSS, UMR CNRS 5616, ICMG FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
authors.forename: Om Prakash
authors.initials: OP
authors.lastname: Edupuganti
authors.forename: Yashveer
authors.initials: Y
authors.lastname: Singh
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
authors.forename: Pascal
authors.initials: P
authors.lastname: Dumy
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D010091:Oximes; D010455:Peptides