Expanding the scope of site-specific protein biotinylation strategies using small molecules.
We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods.
Tan
Lay-Pheng
LP
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Chen
Grace Y J
GY
Yao
Shao Q
SQ
D001665:Binding Sites / Q000502:physiology; D019921:Biotinylation / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism*
D011506:Proteins