Decrease of immobility behavior in forced-swimming test and immune system enhancing effect of traditional medicine Gamisipjundaebo-tang.
Gamisipjundaebo-tang (GSDBT) has been used for the purpose of development of physical strength. In the present study, we investigated the immune enhancing effect induced by GSDBT. We investigated the anti-immobility effect of GSDBT via a forced-swimming test and blood biochemical parameters related to fatigue, glucose, blood urea nitrogen, lactic dehydrogenase, creatine kinase, and total protein. GSDBT (0.1 and 1 g/kg) was orally administered to mice for 14 days. After 7 and 14 days, as assessed through a forced-swimming test, immobility time was decreased in the GSDBT-administrated group (0.1 and 1 g/kg) in comparison with the control group. In addition, after 8 days, the contents of glucose and lactate dehydrogenase in the blood serum were increased, and contents of blood urea nitrogen were decreased in the GSDBT-administrated group. After 15 days, the contents of glucose were increased, and the contents of lactate dehydrogenase and blood urea nitrogen were decreased in the GSDBT-administrated group. However, it had no effect on the elevation of creatine kinase and total protein level. We also investigated the effect of GSDBT on the production of cytokines in human T-cell line, MOLT-4 cells, and splenocytes. GSDBT significantly increased interferon (IFN)-gamma and interleukin (IL)-2 levels compared with the media control but did not affect IL-4. GSDBT increased the protein expression of IFN-gamma in MOLT-4 cells. These results suggest that GSDBT may be useful in immune function improvement and may also have antifatigue properties.
Shin
Hye-Young
HY
College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea.
Shin
Tae-Yong
TY
Seo
Sang-Wan
SW
Na
Ho-Jeong
HJ
Kwon
Yong-Taek
YT
Song
Bong-Keun
BK
Lee
Eon-Jeong
EJ
Kim
Yun-Kyung
YK
Hong
Moo-Chang
MC
Shin
Min-Kyu
MK
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
D000818:Animals; D002460:Cell Line; D005221:Fatigue / Q000188:drug therapy; D006801:Humans; D007103:Immobilization; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010946:Plants, Medicinal; D013550:Swimming; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D007155:Immunologic Factors; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma