Prosthetic radioiodination of interleukin-8 ([(123/131)I]-IL-8): biological behavior in a mouse infection model.
Numerous molecular entities with diverse structures have been radiolabeled and investigated as potential infection and inflammation detection agents. However, none of these molecules have gained the acceptance of gallium citrate or radiolabeled autologous white blood cells. We have radioiodinated interleukin-8 using two different methods and tested the biological behavior of the products in mice. As expected, the direct radioiodinated material displayed extensive in vivo deiodination. The use of pyridine-based prosthetic label yielded a product with better kinetics than the direct radioiodination method and showed a better target to non-target ratio. Nonetheless, this method is not suited for labeling of bioactive peptides such as the title peptide because of the very high specific activity required to prevent cytotoxic effects in a human application.
Amartey
J K
JK
Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, MBC-03, P.O. Box 3354, Riyadh 11211, Kingdom of Saudi Arabia. amarty@kfshrc.edu.sa
Esguerra
C
C
Al-Otaibi
B
B
Al-Jammaz
I
I
Al-Qahtani
M
M
Parhar
R S
RS
D000818:Animals; D002478:Cells, Cultured; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging* / Q000378:metabolism*; D016209:Interleukin-8 / Q000737:chemistry / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods; D008657:Metabolic Clearance Rate; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils / Q000000981:diagnostic imaging* / Q000378:metabolism*; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D014018:Tissue Distribution
D016209:Interleukin-8; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals