title: Methods to study protein-protein interactions.
abstract: Protein-protein interactions are the underpinnings of a vast number of cellular processes. In recent years, the convergence of biochemistry, cellular, and molecular biology has made available a number of powerful techniques for studying such interactions. These techniques vary in their sensitivity, efficiency, and rapidity, but judicial deployment of a combination of them has proved to be effective and reliable. Here, we highlight a version of the yeast two-hybrid assay originally pioneered by Fields and Song (1989) and subsequently enhancements by other investigators. We also briefly describe a number of new fluorescent imaging-based biophysical techniques for studying protein-protein interactions FRET, FCS, and BiFC. Together, these constitute an impressive collection of tools for studying interactions among proteins.
authors.affiliation: Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinati College of Medicine, OH, USA.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Meng
authors.forename: Meghan
authors.initials: M
authors.lastname: Rojas
authors.forename: Willis
authors.initials: W
authors.lastname: Bacon
authors.forename: John T
authors.initials: JT
authors.lastname: Stickney
authors.forename: Wallace
authors.initials: W
authors.lastname: Ip
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D012867:Skin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D020798:Two-Hybrid System Techniques
chemical_list: D005456:Fluorescent Dyes