Insulin degrading enzyme is expressed in the human cerebrovascular endothelium and in cultured human cerebrovascular endothelial cells.
Insulin degrading enzyme (IDE) is found in the cytosol, peroxisomes and plasma membrane of many cells. Although it preferentially cleaves insulin it can also cleave many other small proteins with diverse sequences including the monomeric form of the amyloid beta peptide (A beta). In the brain, IDE has been reported to be expressed predominantly in neurons. In this study, IDE expression was detected in cultured human cerebrovascular endothelial cells. Using laser capture microdissection followed by PCR analysis, it was found that IDE mRNA is expressed in human brain blood vessels. Using immunofluorescence and multiphoton microscopy IDE was localized to the endothelium of the cerebrovascular blood vessels in human.
Gao
Wenwu
W
ENR VA Medical Center, Bedford, MA 01730, USA.
Eisenhauer
Patricia B
PB
Conn
Kelly
K
Lynch
John A
JA
Wells
John M
JM
Ullman
M David
MD
McKee
Ann
A
Thatte
Hamant S
HS
Fine
Richard E
RE
D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007339:Insulysin / Q000235:genetics* / Q000378:metabolism*; D008833:Microcirculation; D013378:Substantia Nigra / Q000098:blood supply
D007339:Insulysin