Methods for gene expression profiling in dermatology research using DermArray nylon filter DNA microarrays.
Here we present methods of gene expression profiling using nylon filter deoxyribonucleic acid (DNA) microarrays and radiolabeled and nonradiolabeled hybridization probes. DermArray(R) nylon filter DNA microarrays were designed specifically for use in dermatology research. A patent-pending method was used to select approx 4400 highly informative, sequence-verified human cDNA clones for this DNA micro array. Using DermArray(R) filters, biomarkers have been discovered for normal and pathologic cells from skin, and for responses to dermatologic drugs. As an example, gene expression profiling was performed with hydroquinone-treated SKMel-28 cells, a melanoma cell line. Also included are the methods for bioinformatic analysis using Pathwaystrade mark software.
Davis
Richard L
RL
NRC Division, Midwest Research Institute, Rockland, MD, USA.
DuBreuil
Rusla M
RM
Reddy
Shanker P
SP
Dooley
Thomas P
TP
D019295:Computational Biology; D003880:Dermatology; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D012867:Skin / Q000378:metabolism*