title: Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis through bioassay-guided fractionation procedures in rats.
abstract: The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and (1)H, (13)C and 2D NMR spectroscopic techniques.
authors.affiliation: Department of Pharmacognosy, Gazi University, Faculty of Pharmacy, Etiler, 6330 Ankara, Turkey. yesilada@gazi.edu.tr
authors.forename: Erdem
authors.initials: E
authors.lastname: Yesilada
authors.forename: Ilhan
authors.initials: I
authors.lastname: Gürbüz
authors.forename: Erdal
authors.initials: E
authors.lastname: Bedir
authors.forename: Irem
authors.initials: I
authors.lastname: Tatli
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification*; D001681:Biological Assay / Q000379:methods; D031179:Centaurea; D005591:Chemical Fractionation / Q000379:methods*; D005260:Female; D035264:Flowers; D007783:Lactones / Q000302:isolation & purification* / Q000627:therapeutic use; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000627:therapeutic use; D013276:Stomach Ulcer / Q000188:drug therapy
chemical_list: D000897:Anti-Ulcer Agents; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes