Expression of secretory phospholipase A2 enzymes in lungs of humans with pneumonia and their potential prostaglandin-synthetic function in human lung-derived cells.
Although a number of sPLA2 (secretory phospholipase A2) enzymes have been identified in mammals, the localization and functions of individual enzymes in human pathologic tissues still remain obscure. In the present study, we have examined the expression and function of sPLA2s in human lung-derived cells and in human lungs with pneumonia. Group IID, V and X sPLA2s were expressed in cultured human bronchial epithelial cells (BEAS-2B) and normal human pulmonary fibroblasts with distinct requirement for cytokines (interleukin-1b, tumour necrosis factor a and interferon-g). Lentivirus- or adenovirus-mediated transfection of various sPLA2s into BEAS-2B or normal human pulmonary fibroblast cells revealed that group V and X sPLA2s increased arachidonate release and prostaglandin production in both cell types, whereas group IIA and IID sPLA2s failed to do so. Immunohistochemistry of human lungs with pneumonia demonstrated that group V and X sPLA2s were widely expressed in the airway epithelium, interstitium and alveolar macrophages, in which group IID sPLA2 was also positive, whereas group IIA sPLA2 was restricted to the pulmonary arterial smooth muscle layers and bronchial chondrocytes, and group IIE and IIF sPLA2s were minimally detected. These results suggest that group V and X sPLA2s affect lung pathogenesis by facilitating arachidonate metabolism or possibly through other functions.
Masuda
Seiko
S
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Murakami
Makoto
M
Mitsuishi
Michiko
M
Komiyama
Kazuo
K
Ishikawa
Yukio
Y
Ishii
Toshiharu
T
Kudo
Ichiro
I
D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism; D001980:Bronchi / Q000166:cytology / Q000201:enzymology; D001996:Bronchopneumonia / Q000201:enzymology* / Q000473:pathology; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000201:enzymology*; D016676:Macrophages, Alveolar / Q000201:enzymology / Q000378:metabolism; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000473:pathology; D010741:Phospholipases A / Q000096:biosynthesis* / Q000378:metabolism*; D054467:Phospholipases A2; D011651:Pulmonary Artery / Q000201:enzymology / Q000473:pathology; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone