Reversibly switchable DNA nanocompartment on surfaces.
Biological macromolecules have been used to fabricate many nanostructures, biodevices and biomimetics because of their physical and chemical properties. But dynamic nanostructure and biomachinery that depend on collective behavior of biomolecules have not been demonstrated. Here, we report the design of DNA nanocompartments on surfaces that exhibit reversible changes in molecular mechanical properties. Such molecular nanocompartments are used to encage molecules, switched by the collective effect of Watson-Crick base-pairing interactions. This effect is used to perform molecular recognition. Furthermore, we found that 'fuel' strands with single-base variation cannot afford an efficient closing of nanocompartments, which allows highly sensitive label-free DNA array detection. Our results suggest that DNA nanocompartments can be used as building blocks for complex biomaterials because its core functions are independent of substrates and mediators.
Mao
Youdong
Y
Laboratory for Biotechnology, Peking University, Beijing 100871, China. jackmao@water.pku.edu.cn
Luo
Chunxiong
C
Deng
Wei
W
Jin
Guangyin
G
Yu
Xiaomei
X
Zhang
Zhaohui
Z
Ouyang
Qi
Q
Chen
Runsheng
R
Yu
Dapeng
D
D020137:Base Pair Mismatch; D020029:Base Pairing; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
D004247:DNA