[Determination of adenosine and cordycepin in Cordyceps sinensis and C. militarris with HPLC-ESI-MS].
OBJECTIVE
HPLC-ESI-MS to establish a method for simultaneous determination of adenosine and cordycepin in Cordyceps sinensis and C. militarris.


METHODS
HPLC-ESI-MS method. An electrospray ionization (ESI) interface and selective ion monitoring (SIM) mode were used. The analytical column was a 2.0 mm x 150 mm Shimadzu VP - ODS column and the mobile phase was water (94%), methanol (5%) and formic acid (1%). 2-Chloroadenosine was used as internal standard for this assay.


RESULTS
The regression equations and coefficient were Y = 0.134 6X + 0.001 29 (r = 0.998 4) for adenosine, Y = 0.216 4X + 0.021 5 (r = 0.999 1) for cordycepin. The liner range was 0.5 approximately 124.5 microg x mL(-1) and 0.5 approximately 136.5 microg x mL(-1) for adenosine and cordycepin, respectively. The average recoveries of adenosine and cordycepin were 95.8% and 98.1%, respectively.


CONCLUSIONS
This method is highly sensitive, fast and selective, which can be used for the determination of nucleosides in C. sinensis and its substitutes. This method can also be applied for the quality control of above herbs.
Huang
Lan-Fang
LF
Research Center of Modernization of Chinese Herbal Medicines, College of Chemical and Chemical Engineering, Changsha 410083, China.
Guo
Fang-Qiu
FQ
Liang
Yi-Zeng
YZ
Chen
Ben-Mei
BM
D000241:Adenosine / Q000032:analysis*; D000818:Animals; D012831:Bombyx; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry* / Q000145:classification; D003839:Deoxyadenosines / Q000032:analysis*; D007915:Lepidoptera; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization
D003839:Deoxyadenosines; C058120:cordycepin; D000241:Adenosine