AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis.
For the efficient translocation of organic nitrogen, small peptides of two to three amino acids are posited as an important alternative to amino acids. A new transporter mediating the uptake of di- and tripeptides was isolated from Arabidopsis thaliana by heterologous complementation of a peptide transport-deficient Saccharomyces cerevisiae mutant. AtPTR1 mediated growth of S. cerevisiae cells on different di- and tripeptides and caused sensitivity to the phytotoxin phaseolotoxin. The spectrum of substrates recognized by AtPTR1 was determined in Xenopus laevis oocytes injected with AtPTR1 cRNA under voltage clamp conditions. AtPTR1 not only recognized a broad spectrum of di- and tripeptides, but also substrates lacking a peptide bond. However, amino acids, omega-amino fatty acids or peptides with more than three amino acid residues did not interact with AtPTR1. At pH 5.5 AtPTR1 had an apparent lower affinity (K(0.5) = 416 microm) for Ala-Asp compared with Ala-Ala (K(0.5) = 54 microm) and Ala-Lys (K(0.5) = 112 microm). Transient expression of AtPTR1/GFP fusion proteins in tobacco protoplasts showed that AtPTR1 is localized at the plasma membrane. In addition, transgenic plants expressing the beta-glucuronidase (uidA) gene under control of the AtPTR1 promoter demonstrated expression in the vascular tissue throughout the plant, indicative of a role in long-distance transport of di- and tripeptides.
Dietrich
Daniela
D
Molecular Plant Physiology, Institute of Plant Sciences, University of Berne, 3013 Berne, Switzerland.
Hammes
Ulrich
U
Thor
Kathrin
K
Suter-Grotemeyer
Marianne
M
Flückiger
Ricardo
R
Slusarenko
Alan J
AJ
Ward
John M
JM
Rentsch
Doris
D
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018525:Germination; D026901:Membrane Transport Proteins / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D009952:Ornithine / Q000031:analogs & derivatives* / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D013379:Substrate Specificity
D029681:Arabidopsis Proteins; D026901:Membrane Transport Proteins; D009842:Oligopeptides; C495664:Ptr1 protein, Arabidopsis; C021234:phaseolotoxin; D009952:Ornithine