[The effect of growth factors on the proliferation of cultured porcine trabecular meshwork cells].
OBJECTIVE
We attempted to identify cell growth factors that cause a multiplication of trabecular meshwork(TM) cells.


METHODS
Porcine TM cells were cultured in Dulbecco's modified Eagle's medium/nutrient mixture F-12/Ham to which we added 1, 10, and 100 ng/ml of platelet-derived growth factor(PDGF), fibroblast growth factor 2(FGF2), insulin-like growth factor-1 (IGF-1), vascular endothelial cell growth factor(VEGF), hepatocyte growth factor (HGF), or brain-derived neurotrophic factor(BDNF). We measured [3H] thymidine incorporation to evaluate the influence of the growth factors on TM cell proliferation.


RESULTS
[3H] thymidine incorporation into TM cells was promoted by 10 and 100 ng/ml of PDGF, IGF-1, and FGF2 after 24 and 48 hours, whereas 1, 10, and 100 ng/ml of VEGF restrained cell proliferation after 48 hours. HGF and BDNF did not show any remarkable influence on TM cell proliferation.


CONCLUSIONS
Our, results suggest that PDGF, IGF-1, and FGF2 may cause a drop in intraocular pressure followed by activation of a TM function, by multiplying TM cells.
Ando
Akira
A
Department of Ophthalmology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi 570-8507, Japan.
Kaneko
Shiho
S
Matsumura
Miyo
M
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013552:Swine; D014129:Trabecular Meshwork / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D019208:Brain-Derived Neurotrophic Factor; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I