[Effect of inhaled cyclosporin A on antigen-induced airway inflammation in asthmatic rats].
OBJECTIVE
To investigate the effect of inhalation of cyclosporin (CsA) on antigen-induced airway inflammation in Sprague-Dawley rats.


METHODS
Rats were sensitized with antigen (ovalbumin, OA). After two weeks, the sensitized rats were pretreated with aerosol CsA (5, 10, 20 g x L(-1)), once per day for 7 days. Then, the sensitized rats were challenged with OA (10 g x L(-1), once per day) for 2 days at day 20 after sensitization. The number of eosinophils in bronchoalveolar lavage fluid (BALF) and peripheral blood, histological changes of lung tissue, and TNF-alpha content in BALF were investigated.


RESULTS
Inhalation of CsA significantly reduced the number of eosinophils in BALF and peripheral blood, inflammatory infiltration and tissue edema of lung tissue, decreased the content of TNF-alpha in BALF.


CONCLUSIONS
Inhalation of CsA inhibited airway inflammation in rats, and the mechanism is related to inhibition of TNF-alpha release.
Chen
Ying
Y
Nephrology Center, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China. zyb-cy@163.com
Xie
Qiang-Min
QM
Yang
Qiu-Huo
QH
Chen
Ji-Qiang
JQ
D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D004804:Eosinophils / Q000473:pathology*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology*; D008297:Male; D010047:Ovalbumin; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007166:Immunosuppressive Agents; D014409:Tumor Necrosis Factor-alpha; D016572:Cyclosporine; D010047:Ovalbumin