title: [A study of genetic polymorphism of the STR locus D7S2846, D19S400 and D18S535 in Han population in Wenzhou].
abstract: OBJECTIVE
  To study genetic polymorphism of D7S2846, D19S400 and D18S535 in Han population in Wenzhou.
  
  
  METHODS
  DNA was extracted with chelex-100 method from EDTA-blood samples of 194 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.
  
  
  RESULTS
  6 alleles and 15 genotypes of D7S2846, 10 alleles and 36 genotypes of D19S400, 8 alleles and 26 genotypes of D18S535 was observed. The heterozygosities of the three loci are 0.644, 0.724 and 0.772; The power of discriminating are 0.854, 0.940 and 0.938.
  
  
  CONCLUSIONS
  The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium (P>0.05), so the three loci can be used in forensic medicine and in other genetic researches.
authors.affiliation: Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Hong-qin
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Shu-zhen
authors.initials: SZ
authors.lastname: Wu
authors.forename: Yun-tian
authors.initials: YT
authors.lastname: Bi
mesh_terms: D000483:Alleles; D002681:China / Q000208:ethnology; D004247:DNA / Q000302:isolation & purification; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
chemical_list: D004247:DNA