Function of lanGT3, a glycosyltransferase gene involved in landomycin A biosynthesis.
The glycosyltransferase gene lanGT3, involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGT3 in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.
Luzhetskyy
Andriy
A
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Liu
Tao
T
Fedoryshyn
Marta
M
Ostash
Bohdan
B
Fedorenko
Viktor
V
Rohr
Jürgen
J
Bechthold
Andreas
A
D000617:Aminoglycosides / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C064648:landomycin A; D016695:Glycosyltransferases