Immunolocalization of 14-3-3 isoforms in brains with Pick body disease.
Immunolocalization of 14-3-3 protein isoforms in relation to Pick bodies in Pick body disease (PBD) brains was investigated. Weakly granular immunoreactivity of 14-3-3 proteins was found in neurons in control subjects and in Pick body disease brains. In addition to this granular immunoreactivity, many Pick bodies were immunopositive for 14-3-3 proteins as confirmed with double-immunofluorescence with an anti-PHF tau (AT8) and anti-14-3-3 that recognizes all its isoforms (common). When probed with isoform-specific antibodies, Pick bodies were positive for beta, gamma, epsilon, eta, tau, and zeta isoform and exhibited immunostaining pattern similar to that observed with the anti-14-3-3 proteins (common). In addition, immunoreactivity of sigma isoform, so far considered to be exclusively extraneuronal, was unexpectedly found in Pick bodies, normal hippocampal neurons and brain homogenate from age-matched controls. Although localization of 14-3-3 proteins in Pick bodies suggests their involvement in Pick body formation, their role may be variable dependent on the isoforms differently expressed in different area in the brain.
Umahara
Takahiko
T
Department of Geriatrics, Tokyo Medical University, 6-7-1 Nishi-Shinjyuku, Shinjyuku-ku, Tokyo 160-0023, Japan. takahiko@tokyo-med.ac.jp
Uchihara
Toshiki
T
Tsuchiya
Kuniaki
K
Nakamura
Ayako
A
Ikeda
Kenji
K
Iwamoto
Toshihiko
T
Takasaki
Masaru
M
D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007120:Immunochemistry; D008969:Molecular Sequence Data; D020774:Pick Disease of the Brain / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D048948:14-3-3 Proteins; D020033:Protein Isoforms