Positive predictive value of Gen-Probe APTIMA Combo 2 testing for Neisseria gonorrhoeae in a population of women with low prevalence of N. gonorrhoeae infection.
We collected specimens from women who tested positive for Neisseria gonorrhoeae using the Gen-Probe APTIMA Combo 2 (AC2), and we performed confirmatory tests using a nucleic acid amplification test (NAAT) that targets alternate gonococcal nucleic acid sequences. Among 59,664 specimens, 280 (0.47%) had positive results using AC2; 265 of these specimens were tested using the confirmatory NAAT, of which 258 yielded positive results (positive predictive value, 97.4%; 95% confidence interval, 95.1%-98.8%). Routine confirmatory testing of specimens with positive AC2 gonorrhea test results is not indicated.
Golden
Matthew R
MR
Division of Infectious Diseases, University of Washington, Seattle, WA, USA. golden@u.washington.edu
Hughes
James P
JP
Cles
Linda E
LE
Crouse
Karen
K
Gudgel
Katherine
K
Hu
Jinxin
J
Swenson
Paul D
PD
Stamm
Walter E
WE
Handsfield
H Hunter
HH
D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D015995:Prevalence; D012680:Sensitivity and Specificity