title: Repetitive measurements of Chlamydia pneumoniae DNA in peripheral blood mononuclear cells in healthy control subjects and dialysis patients: a prospective study.
abstract: Infection with Chlamydia pneumoniae has been suggested to play a role in the development and maintenance of atherosclerosis. However, the course of C. pneumoniae infection is not clarified. Thus, both the persistence of C. pneumoniae DNA in blood and the tendency to recurrence have not been studied. We determined the prevalence of C. pneumoniae DNA in the white cells of the peripheral blood in 98 dialysis patients and in 52 healthy subjects. Blood samples were collected approximately 6 times from each subject during a period of 1 y with an interval of approximately 2 months and analysed with a polymerase chain reaction. C. pneumoniae DNA was detectable in 47 out of 150 subjects at least once during a y. Reinfection was a rare phenomenon and the presence of C. pneumoniae DNA in blood was of less than 2 months' duration in almost all patients. There was a significant association between the presence of C. pneumoniae DNA during 1 y and the presence of atherosclerosis in the legs of dialysis patients (OR=3.50, p=0.03). Additionally, a significant association was found between the presence of C. pneumoniae DNA and an abnormal electrocardiogram (ECG) (OR=3.16, p=0.01). These findings may support the hypothesis of an association between infection with C. pneumoniae and the presence or development of atherosclerosis.
authors.affiliation: Department of Medicine, Holstebro Hospital, Holstebro and Aarhus University, Denmark. ajam@ringamt.dk
authors.forename: Jakob
authors.initials: J
authors.lastname: Müller
authors.forename: Charlotte
authors.initials: C
authors.lastname: Holm
authors.forename: Ole
authors.initials: O
authors.lastname: Nyvad
authors.forename: Niels A
authors.initials: NA
authors.lastname: Larsen
authors.forename: Erling B
authors.initials: EB
authors.lastname: Pedersen
mesh_terms: D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D023521:Chlamydophila Infections / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D003718:Denmark / Q000453:epidemiology; D005260:Female; D006801:Humans; D015994:Incidence; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011336:Probability; D011446:Prospective Studies; D012008:Recurrence; D012016:Reference Values; D006435:Renal Dialysis / Q000009:adverse effects*; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D017678:Sex Distribution
chemical_list: D004269:DNA, Bacterial