title: Simultaneously monitoring gene expression kinetics and genetic noise in single cells by optical well arrays.
abstract: An optical fiber based well array platform was used for simultaneous, dynamic gene expression monitoring from hundreds of individual live Escherichia coli cells carrying promoter-fluorescent reporter gene fusions. High information content about gene expression kinetics and cell-to-cell gene expression variability can be collected from a single experiment. These data are invaluable for investigating gene regulation and gene networks as well as for systems biology applications.
authors.affiliation: Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA.
authors.forename: Yina
authors.initials: Y
authors.lastname: Kuang
authors.forename: Israel
authors.initials: I
authors.lastname: Biran
authors.forename: David R
authors.initials: DR
authors.lastname: Walt
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D007700:Kinetics; D055095:Optics and Photonics