Feasibility of reduction in use of the mouse bioassay: presence/absence screening for saxitoxin in frozen acidified mussel and oyster extracts from the coast of California with in vitro methods.
In the United States, the detection of paralytic shellfish poisoning (PSP) for regulatory purposes relies on the mouse bioassay (MBA). Using a saxitoxin presence/absence test could reduce animal usage significantly. Three in vitro methods, the RIDASCREEN Saxitoxin kit, MIST Alert, and a 5 h neuroblastoma assay, were evaluated in parallel with the MBA using 106 twice-frozen, acidified extracts from California-grown mussel and oyster tissues. For each assay, a cutoff point was established whereby data below or equal to that point were scored as negative and were assigned a score of zero. Data above the cutoff were considered positive and assigned a score of one. Pearson correlation coefficients were generated. The RIDASCREEN, MIST Alert, and neuroblastoma bioassay correlated to the MBA at 0.849, 0.853, and 0.832 when used for presence/absence detection. These data suggest that a reduction in MBA usage could be achieved in the surveillance of California-grown mussels and oysters for PSP-associated toxins. Correlation data between the in vitro assays, cost comparisons, and the potential for false negatives and false positives were examined. Implications of these methodologies in protecting public health are discussed.
Inami
Gregory B
GB
California Department of Health Services, Microbial Diseases Laboratory, 850 Marina Bay Pkwy, MS E-164, Richmond, CA 94804, USA. ginami@dhs.ca.gov
Crandall
Claudia
C
Csuti
Donna
D
Oshiro
Michael
M
Brenden
Rita A
RA
D000818:Animals; D001681:Biological Assay; D049872:Bivalvia / Q000737:chemistry*; D005628:Frozen Foods; D051379:Mice; D010125:Ostreidae / Q000737:chemistry*; D012530:Saxitoxin / Q000032:analysis*
D012530:Saxitoxin