Immobilization of cholesterol oxidase on cellulose acetate membrane for free cholesterol biosensor development.
This article describes the immobilization of cholesterol oxidase on a cellulose acetate (CA) membrane activated by Sodium periodate, ethylenediamine, and glutaraldehyde etc. The properties of the immobilized enzyme membrane were investigated. The factors affecting the activity of immobilized enzyme such as the concentration of glutaraldehyde, the concentration of enzyme used during immobilization, temperature, pH, and immobilizing time etc. were also studied. The immobilized COD membrane has been used to construct fibre-optic fluorescent biosensor.
Wang
Shenqi
S
Key Laboratory of Bioactive Materials, Ministry of Education Nankai University, Tianjin, P.R. China. sanyiwang@263.net
Li
Shipu
S
Yu
Yaoting
Y
D015374:Biosensing Techniques / Q000379:methods*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005336:Fiber Optic Technology; D005453:Fluorescence; D008567:Membranes, Artificial
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose; D002784:Cholesterol; D002789:Cholesterol Oxidase