[Growth behaviour of human mononuclear cells derived from bone marrow and cord blood on a collagen carrier for osteogenic regeneration].
We investigated the biocompatibility and osteogenetic potency of a porcine collagen I/III carrier in a human bone marrow and cord blood cell culture system.


METHODS
Human mesenchymal mononuclear cells were isolated from cord blood and iliac crest bone marrow and cultivated in various cell densities on a semipermeable porcine collagen I/III carrier. After 14 days of in vitro cultivation both cultures were subjected to osteogenic stimulation by dexamethasone, ascorbic acid and beta-glycerol phosphate (DAG) until day 40. Semiquantitative immunochemical evaluation based on osteoblastic and progenitor cell markers was then done.


RESULTS
With regard to the minimal local cell density required for growth and osteogenic differentiation, cord blood derived progenitor cells showed lower tolerance in comparison with bone marrow derived cells. For both cell culture systems three-dimensional growth and calcification within the collagen fibres were seen after osteogenic stimulation.


CONCLUSIONS
Human cord blood and bone marrow derived mesenchymal stem cell are capable of differentiating into osteoblasts after incubation with a collagen I/III biomaterial.
Wild
A
A
Orthopädische Universitätsklinik Leipzig, Semmelweisstrasse 10, 04103 Leipzig. alexander.wild@medizin.uni-leipzig.de
Jäger
M
M
Lensing-Hoehn
S
S
Werner
A
A
Krauspe
R
R
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D016025:Bone Transplantation / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D024042:Collagen Type I; D024061:Collagen Type III; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007085:Ilium; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering
D001672:Biocompatible Materials; D024042:Collagen Type I; D024061:Collagen Type III; D003470:Culture Media