title: A comprehensive approach for establishment of the platform to analyze functions of KIAA proteins II: public release of inaugural version of InGaP database containing gene/protein expression profiles for 127 mouse KIAA genes/proteins.
abstract: The inaugural version of the InGaP database (Integrative Gene and Protein expression database; http://www.kazusa.or.jp/ingap/index.html) is a comprehensive database of gene/protein expression profiles of 127 mKIAA genes/proteins related to hypothetical ones obtained in our ongoing cDNA project. Information about each gene/protein consists of cDNA microarray analysis, subcellular localization of the ectopically expressed gene, and experimental data using anti-mKIAA antibody such as Western blotting and immunohistochemical analyses. KIAA cDNAs and their mouse counterparts, mKIAA cDNAs, were mainly isolated from cDNA libraries derived from brain tissues, thus we expect our database to contribute to the field of neuroscience. In fact, cDNA microarray analysis revealed that nearly half of our gene collection is predominantly expressed in brain tissues. Immunohistochemical analysis of the mouse brain provides functional insight into the specific area and/or cell type of the brain. This database will be a resource for the neuroscience community by seamlessly integrating the genomic and proteomic information about the mouse KIAA genes/proteins.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. hkoga@kazusa.or.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koga
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yuasa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagase
authors.forename: Kiyo
authors.initials: K
authors.lastname: Shimada
authors.forename: Mihoko
authors.initials: M
authors.lastname: Nagano
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Imai
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakajima
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Murakami
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawai
authors.forename: Futaba
authors.initials: F
authors.lastname: Miki
authors.forename: Junji
authors.initials: J
authors.lastname: Magae
authors.forename: Susumu
authors.initials: S
authors.lastname: Inamoto
authors.forename: Noriko
authors.initials: N
authors.lastname: Okazaki
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D005544:Forecasting; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D023281:Genomics; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D036103:Nanotechnology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D000073718:Pancreatitis-Associated Proteins; D040901:Proteomics; D012984:Software; D013045:Species Specificity; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human