title: A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice.
abstract: The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7.
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Conley
authors.forename: V
authors.initials: V
authors.lastname: Nduati
authors.forename: J L
authors.initials: JL
authors.lastname: Gonzalez-Hernandez
authors.forename: A
authors.initials: A
authors.lastname: Mesfin
authors.forename: M
authors.initials: M
authors.lastname: Trudeau-Spanjers
authors.forename: S
authors.initials: S
authors.lastname: Chao
authors.forename: G R
authors.initials: GR
authors.lastname: Lazo
authors.forename: D D
authors.initials: DD
authors.lastname: Hummel
authors.forename: O D
authors.initials: OD
authors.lastname: Anderson
authors.forename: L L
authors.initials: LL
authors.lastname: Qi
authors.forename: B S
authors.initials: BS
authors.lastname: Gill
authors.forename: B
authors.initials: B
authors.lastname: Echalier
authors.forename: A M
authors.initials: AM
authors.lastname: Linkiewicz
authors.forename: J
authors.initials: J
authors.lastname: Dubcovsky
authors.forename: E D
authors.initials: ED
authors.lastname: Akhunov
authors.forename: J
authors.initials: J
authors.lastname: Dvorák
authors.forename: J H
authors.initials: JH
authors.lastname: Peng
authors.forename: N L V
authors.initials: NL
authors.lastname: Lapitan
authors.forename: M S
authors.initials: MS
authors.lastname: Pathan
authors.forename: H T
authors.initials: HT
authors.lastname: Nguyen
authors.forename: X-F
authors.initials: XF
authors.lastname: Ma
authors.lastname: Miftahudin
authors.forename: J P
authors.initials: JP
authors.lastname: Gustafson
authors.forename: R A
authors.initials: RA
authors.lastname: Greene
authors.forename: M E
authors.initials: ME
authors.lastname: Sorrells
authors.forename: K G
authors.initials: KG
authors.lastname: Hossain
authors.forename: V
authors.initials: V
authors.lastname: Kalavacharla
authors.forename: S F
authors.initials: SF
authors.lastname: Kianian
authors.forename: D
authors.initials: D
authors.lastname: Sidhu
authors.forename: M
authors.initials: M
authors.lastname: Dilbirligi
authors.forename: K S
authors.initials: KS
authors.lastname: Gill
authors.forename: D W
authors.initials: DW
authors.lastname: Choi
authors.forename: R D
authors.initials: RD
authors.lastname: Fenton
authors.forename: T J
authors.initials: TJ
authors.lastname: Close
authors.forename: P E
authors.initials: PE
authors.lastname: McGuire
authors.forename: C O
authors.initials: CO
authors.lastname: Qualset
authors.forename: J A
authors.initials: JA
authors.lastname: Anderson
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D011003:Ploidies; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics*