Effects of PCB99 and PCB153 exposure on spermatogenesis in young adult C57BL6 mice.
This study examined the effects of acute exposure to PCB99 (2,2',4,4',5-pentachlorobiphenyl), and PCB153 (2,2',4,4'5,5'-hexachlorobiphenyl), on spermatogenesis in 8-week-old C57BL6 mice. The mice were randomly allocated to PCB99 and PCB153 and a single dose of respectively 10 and 100 mg/kg was given by oral gavage. During the 6-week experiment, six mice per treatment group were sacrificed weekly, body weights were recorded and samples with respect to the male reproductive system were collected until further analysis. None of the treatments, showed changes in body weight or reproductive endpoints. Flow cytometric analysis revealed spermatogenesis to be unaffected. However, PCB99 and PCB153 showed a significant increase in Leydig cell apoptosis. The results from the present study indicate that the male reproductive system is relatively refractory to PCB99 and PCB153 at levels exceeding those of wildlife and humans, when exposed during adult life. However, the finding of apoptotic Leydig cells merits further investigation.
Oskam
Irma C
IC
Department of Production Animal Clinical Science, Norwegian School of Veterinary Science, P.O. Box 8146, NO033 Oslo, Norway.
Ropstad
Erik
E
Smith
Adrian J
AJ
Skaare
Janneche U
JU
Tverdal
Aage
A
Berg
Kjell Andersen
KA
Wiger
Richard
R
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D005837:Genitalia, Male / Q000187:drug effects / Q000473:pathology; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000633:toxicity*; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D023382:Toxicity Tests, Acute
D004785:Environmental Pollutants; C500336:2,2',4,4',5-pentachlorobiphenyl; D013739:Testosterone; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl