Preparation of reversibly glucose-responsive hydrogels by covalent immobilization of lectin in polymer networks having pendant glucose.
Glucose-responsive hydrogels were prepared by copolymerization of a monomer having a pendant glucose with modified lectin (concanavalin A (ConA)) having vinyl groups. Swelling behavior of ConA-copolymerized glucosyloxyethyl methacrylate (GEMA) hydrogels was discussed from the viewpoint of their synthetic condition and structures. The swelling ratio of the ConA-copolymerized GEMA hydrogels was strongly dependent on the glucose concentration in a buffer solution. As free glucose resulted in the dissociation of the complex between ConA and pendant glucose in the networks and the cross-linking density in the hydrogel decreased, the hydrogels swelled gradually in the presence of free glucose. Leak of ConA from the ConA-entrapment hydrogel and ConA-copolymerized hydrogel was examined in order to discuss the reversible changes of their glucose-responsive behavior. During swelling in the presence of free glucose, ConA leaked out of the ConA-entrapment GEMA hydrogel but did not out of the ConA-copolymerized GEMA hydrogel. As a result, the ConA-copolymerized GEMA hydrogel showed the reversible swelling changes in response to a stepwise change in the glucose concentration. This study revealed that covalent immobilization of ConA in the GEMA networks is very important for development of reversibly glucose-responsive hydrogels.
Miyata
Takashi
T
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. tmiyata@ipcku.kansai-u.ac.jp
Jikihara
Atsushi
A
Nakamae
Katsuhiko
K
Hoffman
Allan S
AS
D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000302:isolation & purification; D003208:Concanavalin A; D005947:Glucose / Q000737:chemistry; D005960:Glucosides / Q000138:chemical synthesis* / Q000302:isolation & purification; D020100:Hydrogels / Q000138:chemical synthesis* / Q000302:isolation & purification; D037102:Lectins; D011108:Polymers / Q000138:chemical synthesis* / Q000302:isolation & purification
D001672:Biocompatible Materials; D005960:Glucosides; D020100:Hydrogels; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A; C090633:poly(glucosylethyl methacrylate); D005947:Glucose