title: [Combination of direct-ELISA and PCR for the rapid detection and identification of Salmonella spp].
abstract: OBJECTIVE
  To develop a protocol for the rapid detection of Salmonellae.
  
  
  METHODS
  A mono-antibody-based direct-ELISA and PCR methods for the detection of Salmonella were developed previously. This study assessed the accuracy of both direct-ELISA and PCR methods for the rapid detection of Salmonella and set up a new detection protocol.
  
  
  RESULTS
  The sensitivity of the PCR method was higher than that of direct-ELISA method. In the 2002 spring physical examination for employees, 1 546 human fecal samples were examined by the combination of direct-ELISA and PCR method. Compared with the results of national standard method, the sensitivity and specificity of direct ELISA was 100% and 97.14%, respectively, while those of PCR method reached both 100%. It also indicated that combination use of two methods could give positive report within 40 hrs, and also achieve high sensitivity and specificity.
  
  
  CONCLUSIONS
  Based on the results obtained, a protocol for the rapid detection of Salmonella was developed. The first step is to us direct-ELISA method to screen the large number of samples, and then use PCR method to validate the ELISA positive samples, and the final step is, if needed, is to use the national standard method to determine the serotypes of Salmonellae.
authors.affiliation: Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China.
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Huang
authors.forename: Xin-An
authors.initials: XA
authors.lastname: Jiao
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Pan
authors.forename: Qi-Yi
authors.initials: QY
authors.lastname: Wen
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Xiu-Fan
authors.initials: XF
authors.lastname: Liu
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012703:Serotyping