Intein-mediated purification of a recombinantly expressed peptide.
A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.
Pezza
John A
JA
Department of Biology, Boston University, 5 Cummington Street, Boston, MA, USA.
Allen
Karen N
KN
Tolan
Dean R
DR
D006863:Hydrogen-Ion Concentration; D047668:Inteins; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature
D010455:Peptides; D011993:Recombinant Fusion Proteins