title: Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes.
abstract: Using gene targeting, we inserted a high-affinity variant of the reverse tetracycline controlled transactivator (rtTA) into the genomic Sox10 locus. This rtTA transgene faithfully recapitulated Sox10 expression in the emerging neural crest, several of its derivatives, and in oligodendrocytes. It was furthermore able to induce expression of a tetracycline inducible transgenic reporter gene in a doxycycline-dependent manner. Induction was fast, with substantial reporter gene expression visible 6 h after the onset of doxycycline treatment. Shut-off, in contrast, exhibited delayed kinetics, which probably correlated with doxycycline clearance rates. This mouse provides a useful tool for generating tetracycline-controlled gene expression in neural crest and oligodendrocytes.
authors.affiliation: Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, 91054 Erlangen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Ludwig
authors.forename: Beate
authors.initials: B
authors.lastname: Schlierf
authors.forename: Anke
authors.initials: A
authors.lastname: Schardt
authors.forename: Klaus-Armin
authors.initials: KA
authors.lastname: Nave
authors.forename: Michael
authors.initials: M
authors.lastname: Wegner
mesh_terms: D000818:Animals; D002460:Cell Line; D003433:Crosses, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018390:Gene Targeting; D006609:High Mobility Group Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D019426:Integrases / Q000235:genetics; D007700:Kinetics; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D009432:Neural Crest / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D055757:SOXE Transcription Factors; D013752:Tetracycline / Q000494:pharmacology*; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics*; D014157:Transcription Factors; D019076:Transgenes; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D006609:High Mobility Group Proteins; D009363:Neoplasm Proteins; D011500:Protein Synthesis Inhibitors; C527923:SOX10 protein, human; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase; D013752:Tetracycline