Isolated small rat hepatocytes express both annexin III and terminal differentiated hepatocyte markers, tyrosine aminotransferase and tryptophan oxygenase, at the mRNA level.
We recently showed that annexin III is expressed in isolated small rat hepatocytes but, not in parenchymal hepatocytes. In the present study, we used reverse transcription polymerase chain analysis to examine the annexin III mRNA level in isolated small rat hepatocytes and parenchymal hepatocytes. Annexin III mRNA was detected in isolated small hepatocytes, but not in isolated parenchymal hepatocytes, confirming the presence of annexin III expression in isolated small rat hepatocytes at the mRNA level and indicating that the absence of annexin III expression in isolated parenchymal hepatocytes is due to the absence of annexin III mRNA. Furthermore, we examined the mRNA level of tyrosine aminotransferase and tryptophan oxygenase, two terminally differentiated hepatocyte markers. mRNA for these markers was detected in both parenchymal hepatocytes and small hepatocytes.
Niimi
Shingo
S
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan. niimi@nihs.go.jp
Hyuga
Masashi
M
Harashima
Mizuho
M
Seki
Taiichiro
T
Ariga
Toyohiko
T
Kawanishi
Toru
T
Hayakawa
Takao
T
D000818:Animals; D017318:Annexin A3 / Q000096:biosynthesis* / Q000235:genetics; D015415:Biomarkers; D002454:Cell Differentiation; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014366:Tryptophan Oxygenase / Q000096:biosynthesis* / Q000235:genetics; D014444:Tyrosine Transaminase / Q000096:biosynthesis* / Q000235:genetics
D015415:Biomarkers; D012333:RNA, Messenger; D014366:Tryptophan Oxygenase; D014444:Tyrosine Transaminase; D017318:Annexin A3