[Some properties of a mutant of Streptomyces globisporus 1912 supersensitive to ultraviolet radiation].
A well-sporulating mutant Spo 1 has been isolated after the action of defective phage from the zone of total lysis of Streptomyces globisporus 1912. It has been shown, that mutant Spo 1 is supersensitive to the action of ultraviolet radiation and has the increased sensitivity to the action of methyl-methane-sulfonate, has mutation in the cluster of genes of synthesis of landomycin E and is not sensitive to the action of nitrous acid and hydrogen peroxide. Spo 1 also has a high level of UV-mutability. After the action of UV on spores 4620 colonies were analysed and 17 auxotrophic mutants (0.36%) were selected. Four auxotrophs restored their ability to produce landomycin E and a partial block of synthesis of the antibiotic was observed in six auxotrophs. Seven of white (lnd E) auxotrophs were analysed with the help of a system "secretor-conventer" and it has been shown that two mutants do not synthesize daidzeine flavonoide and have genotype lnd E dai aux uvs, five of them were capable to synthesize this flavonoide and have genotype lnd dai(+) aux uvs. Initial species of S. globisporus 1912 has increased sensitivity to lethal action of UV. It is supposed that mutant Spo 1 bears two uvs-mutations.
Lavrinchuk
V Ia
VIa
Matseliukh
B P
BP
Tymchyk
O V
OV
D000617:Aminoglycosides / Q000096:biosynthesis; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009154:Mutation; D013171:Spores, Bacterial / Q000187:drug effects / Q000528:radiation effects; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D000617:Aminoglycosides; C113593:landomycin E; D008741:Methyl Methanesulfonate