title: Thrombospondin-1 expression in relation to p53 status and VEGF expression in human breast cancers.
abstract: The aim of the present study was to study the expression and relationship of potential angiogenic factors. Paraffin-embedded tumour sections from 261 breast cancer patients were stained immunohistochemically for thrombospondin (TSP-1) expression. p53 status was previously determined by cDNA-based sequencing, and vascular endothelial growth factor (VEGF) protein expression had been previously analysed using an immunoassay. 241 cancers (92%) had detectable levels of TSP-1. No associations between TSP-1 and p53 status or VEGF were found. No correlations between TSP-1 and relapse-free (P=0.3), breast cancer-corrected (P=0.2) or overall survival (P=0.5) were found. A correlation was found for patients with p53 mutations, but negative p53 expression, with higher VEGF levels (P=0.009), but there was no correlation between this p53 group and those with low TSP-1 levels (P=0.2). In conclusion, TSP-1 expression was not prognostic and was not associated with neither p53-status or VEGF expression.
authors.affiliation: Department of Oncology and Pathology, CCK Cancer Centrum Karolinska, Karolinska Institutet and Hospital, SE-171 76 Stockholm, Sweden. barbro.linderholm@cck.ki.se
authors.forename: Barbro
authors.initials: B
authors.lastname: Linderholm
authors.forename: Eva
authors.initials: E
authors.lastname: Karlsson
authors.forename: Sigrid
authors.initials: S
authors.lastname: Klaar
authors.forename: Thomas
authors.initials: T
authors.lastname: Lindahl
authors.forename: Anna-Lena
authors.initials: AL
authors.lastname: Borg
authors.forename: Göran
authors.initials: G
authors.lastname: Elmberger
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism; D011379:Prognosis; D019700:Thrombospondin 1 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D019700:Thrombospondin 1; D016159:Tumor Suppressor Protein p53; D042461:Vascular Endothelial Growth Factor A