title: [Study on the interface of human hepatocyte L-02 polypropylene:simple culture method of human hepatocyte with spheroidal aggregate culture].
abstract: OBJECTIVE
  To found new interface of human hepatocyte/poly propylene with good cytocompatibility for made polypropylene hollow fibers bioreactor of bioartificial liver in future.
  
  
  METHODS
  Using the macromolecular hydroperoxide groups on the polypropylene membrane surface as initiators, acrylamides were polymerized on the polypropylene membranes, under induction by both UV irradiation and Fe2+ reduction. Growth characteristics of human hepatocyte L-02 were detected when it was cultured on polystyrene, polypropylene and modified polypropylene membrane surface.
  
  
  RESULTS
  Water contact angle measurement of the polypropylene and the modified polypropylene membranes decreased from (72 +/- 5) degrees to (30 +/- 4) degrees , which indicated that the hydrophilicity of the membrane was improved obviously after the grafting modification. Human hepatocyte L-02 could not adhere and spread on modified polypropylene membrane surface, and grown in spheroidal aggregate with higher density and higher proliferation ratio measured by MTT method.
  
  
  CONCLUSIONS
  Acrylamide polymerized on the polypropylene membranes is a good method which not only improved human hepatocytes cytocompatibility but also found a new simple culture method with spheroidal aggregate culture of human hepatocyte.
authors.affiliation: Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Cheng-hong
authors.initials: CH
authors.lastname: Peng
authors.forename: Bao-san
authors.initials: BS
authors.lastname: Han
authors.forename: Chang-you
authors.initials: CY
authors.lastname: Gao
authors.forename: Zu-wei
authors.initials: ZW
authors.lastname: Ma
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Gui-di
authors.initials: GD
authors.lastname: Zhang
authors.forename: Mei-juan
authors.initials: MJ
authors.lastname: Yang
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D019164:Liver, Artificial; D008567:Membranes, Artificial; D011126:Polypropylenes / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D011126:Polypropylenes