[Study on gene expression of Tamarix under NaHCO3 stress using SSH technology].
The gene expression of Tamarix androssowii under NaHCO3 stresses is studied by using SSH technique, in which the cDNA from the materials treated with NaHCO3 solution is as tester and the cDNA from the materials in normal growth is as driver. Total 36 genes related to NaHCO3 stress were obtained through Northern hybridization. Blastx analysis showed that the proteins encoded by these genes were homologous to the following proteins: the antioxidant enzymes catalase and peroxiredoxin; trehalose phosphatase, which was related to trehalose synthesis; a few regulation proteins such as bZIP transcription factor, MADS-box protein, glycine-rich RNA-binding proteins, CCCH-type zinc finger protein and F-box protein etc; early light-induced protein, which could protect and/or repair the photosynthetic apparatus damage induced by stress; cysteine proteinase and vacuolar processing enzyme that can make function in plant cell death, and lipid transfer protein precursor, polyubiquitin, chalcone synthase, NADP-dependent isocitrate dehydrogenase, salt-induced S12 protein, and oxygen-evolving enhancer protein 1 etc. Among 36 genes obtained, the proteins encoded three genes were homologous to 3 putative proteins: HAK2, calcium-binding protein and RNA-binding protein, respectively. In addition, 6 new salt stress response squences were found. The result indicated that the salt-tolerant mechanism of Tamarix androssowii may be a complicated, interactive system involving multiple approaches and multiple genes, but not only a single salt gland-depended approach.
Yang
Chuan-Ping
CP
Forest Resource and Environment College, Northeast Forestry University, Harbin 150040, China. yangcp@mail.nefu.edu.cn
Wang
Yu-Cheng
YC
Liu
Gui-Feng
GF
Jiang
Jing
J
D015870:Gene Expression; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D032363:Tamaricaceae / Q000235:genetics*
D017693:Sodium Bicarbonate