Flow injection chemiluminescence determination of dihydralazine sulphate based on permanganate oxidation sensitized by rhodamine B.
A novel flow injection chemiluminescence (CL) method for the determination of dihydralazine sulphate (DHZS) is described. The method is based on the CL produced during the oxidation of DHZS by acidic permanganate solution in the presence of rhodamine B. Rhodamine B is suggested as a fluorescing compound for the energy-transferred excitation. The CL emission allows quantitation of DHZS concentration in the range 5-800 ng/mL, with a detection limit of 1.9 ng/mL (3sigma). The experimental conditions for the CL reaction are optimized and the possible reaction mechanism is discussed. The method has been applied to the determination of DHZS in pharmaceutical preparations and compares well with the high performance liquid chromatography (HPLC) method.
Yang
Xiao-Feng
XF
Institute of Analytical Science, Northwest University, Xi'an 710069, People's Republic of China.
Wu
Dong-Bing
DB
Li
Hua
H
D000143:Acids; D000959:Antihypertensive Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004078:Dihydralazine / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D011196:Potassium Permanganate / Q000737:chemistry*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry*; D012996:Solutions; D013607:Tablets
D000143:Acids; D000959:Antihypertensive Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D012235:Rhodamines; D012996:Solutions; D013607:Tablets; D011196:Potassium Permanganate; C029773:rhodamine B; D004078:Dihydralazine