title: Clonal relationship of recent invasive Haemophilus influenzae serotype f isolates from Denmark and the United States.
abstract: Surveillance performed after the introduction of general Haemophilus influenzae serotype b (Hib) vaccination in Denmark identified 13 cases of invasive bacteraemic H. influenzae serotype f (Hif) disease in adults over a period of 7 years. Bacteraemic respiratory tract infections accounted for 61 % of cases, but meningitis, epiglottitis and osteoarthritis were also seen. Recent Danish isolates were compared to recent American isolates, historical Hif strains and non-Hif invasive strains. Results of conventional serotyping were confirmed by PCR detection of the serotype-f-specific cap and bexA gene sequences. Multilocus enzyme electrophoresis typing revealed that recent Danish and American isolates belonged to a single Hif clone, which may be undergoing expansion. The need for accurate serotyping of H. influenzae to enable reliable monitoring for Hib replacement by other capsular types is emphasized.
authors.affiliation: Department of Clinical Microbiology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark 2Department of Clinical Microbiology, Hillerød Hospital, Helsevej 2, DK 3400 Hillerød, Denmark 3Department of Clinical Microbiology, Odense University Hospital, J. B. Winsløvsvej 21.2, DK 5000 Odense C, Denmark 4Department of Clinical Microbiology, Hvidovre Hospital, Kettegaard Alle 30, DK 2650 Hvidovre, Denmark 5Department of Medical Microbiology and Immunology, Århus University, Universitetsparken, DK 8000 Århus C, Denmark.
authors.forename: Brita
authors.initials: B
authors.lastname: Bruun
authors.affiliation: Department of Clinical Microbiology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark 2Department of Clinical Microbiology, Hillerød Hospital, Helsevej 2, DK 3400 Hillerød, Denmark 3Department of Clinical Microbiology, Odense University Hospital, J. B. Winsløvsvej 21.2, DK 5000 Odense C, Denmark 4Department of Clinical Microbiology, Hvidovre Hospital, Kettegaard Alle 30, DK 2650 Hvidovre, Denmark 5Department of Medical Microbiology and Immunology, Århus University, Universitetsparken, DK 8000 Århus C, Denmark.
authors.forename: Bente
authors.initials: B
authors.lastname: Gahrn-Hansen
authors.affiliation: Department of Clinical Microbiology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark 2Department of Clinical Microbiology, Hillerød Hospital, Helsevej 2, DK 3400 Hillerød, Denmark 3Department of Clinical Microbiology, Odense University Hospital, J. B. Winsløvsvej 21.2, DK 5000 Odense C, Denmark 4Department of Clinical Microbiology, Hvidovre Hospital, Kettegaard Alle 30, DK 2650 Hvidovre, Denmark 5Department of Medical Microbiology and Immunology, Århus University, Universitetsparken, DK 8000 Århus C, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Westh
authors.affiliation: Department of Clinical Microbiology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark 2Department of Clinical Microbiology, Hillerød Hospital, Helsevej 2, DK 3400 Hillerød, Denmark 3Department of Clinical Microbiology, Odense University Hospital, J. B. Winsløvsvej 21.2, DK 5000 Odense C, Denmark 4Department of Clinical Microbiology, Hvidovre Hospital, Kettegaard Alle 30, DK 2650 Hvidovre, Denmark 5Department of Medical Microbiology and Immunology, Århus University, Universitetsparken, DK 8000 Århus C, Denmark.
authors.forename: Mogens
authors.initials: M
authors.lastname: Kilian
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000368:Aged; D016470:Bacteremia / Q000382:microbiology; D016667:Bacterial Capsules; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002761:Cholangitis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D003718:Denmark; D004798:Enzymes / Q000032:analysis; D004826:Epiglottitis / Q000382:microbiology; D005260:Female; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008100:Liver Abscess / Q000382:microbiology; D008297:Male; D008583:Meningitis, Haemophilus / Q000382:microbiology; D008875:Middle Aged; D017720:Molecular Epidemiology; D010003:Osteoarthritis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000235:genetics; D012141:Respiratory Tract Infections / Q000382:microbiology; D012703:Serotyping; D014481:United States
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004798:Enzymes; D011134:Polysaccharides; C438052:capsular polysaccharide, haemophilus influenzae type F; C068101:bexA protein, Haemophilus influenzae