Insulinomimetic effects of kaempferitrin on glycaemia and on 14C-glucose uptake in rat soleus muscle.
Bauhinia forficata is one of the Bauhinia species mostly used as an antidiabetic herbal remedy in Brazil. Kaempferitrin (kaempferol-3,7-O-(alpha)-L-dirhamnoside) is the predominant flavonol glycoside found in the B. forficata leaves. The aim of the present work was to study the long-term effect of kaempferitrin on glycaemia in diabetic rats, as well as the in vitro effect of this compound on 14C-D-glucose uptake and 14C-leucine incorporation into protein in normal rat soleus muscle. Kaempferitrin was found to have an acute lowering effect on blood glucose in diabetic rats and to stimulate the glucose uptake percentile, as efficiently as insulin in muscle from normal rats. This compound did not have any effect on glucosuria or on protein synthesis in muscle from normal and diabetic animals. However, the protein synthesis in the kaempferitrin-treated groups was maintained at the same level as the respective controls. Thus, the hypoglycaemic effect and the prompt efficiency of the kaempferitrin in stimulating [U-14C]-2-deoxi-D-glucose uptake in muscle -constitute the first evidence to indicate that the acute effect of this compound on blood glucose lowering may occur as a consequence of the altered intrinsic activity of the glucose transporter (Vmax or glucose transporters translocation?) not involving directly the synthesis of new carriers.
Jorge
Ana Paula
AP
Departamento de Bioquímica, Centro de Ciências Biológicas, UFSC, Campus Universitário, Bairro Trindade. Cx. Postal 5069, CEP: 88040-970, Florianópolis, SC, Brazil.
Horst
Heros
H
de Sousa
Eliandra
E
Pizzolatti
Moacir Geraldo
MG
Silva
Fátima Regina Mena Barreto
FR
D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002250:Carbon Radioisotopes; D003847:Deoxyglucose / Q000493:pharmacokinetics; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D004347:Drug Interactions; D006029:Glycosuria / Q000188:drug therapy; D007328:Insulin / Q000494:pharmacology*; D044949:Kaempferols / Q000494:pharmacology*; D007930:Leucine / Q000493:pharmacokinetics; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D002250:Carbon Radioisotopes; D007328:Insulin; D044949:Kaempferols; D003847:Deoxyglucose; D007930:Leucine; C042728:lespenefril