title: Evolutionarily conserved regions and hydrophobic contacts at the superfamily level: The case of the fold-type I, pyridoxal-5'-phosphate-dependent enzymes.
abstract: The wealth of biological information provided by structural and genomic projects opens new prospects of understanding life and evolution at the molecular level. In this work, it is shown how computational approaches can be exploited to pinpoint protein structural features that remain invariant upon long evolutionary periods in the fold-type I, PLP-dependent enzymes. A nonredundant set of 23 superposed crystallographic structures belonging to this superfamily was built. Members of this family typically display high-structural conservation despite low-sequence identity. For each structure, a multiple-sequence alignment of orthologous sequences was obtained, and the 23 alignments were merged using the structural information to obtain a comprehensive multiple alignment of 921 sequences of fold-type I enzymes. The structurally conserved regions (SCRs), the evolutionarily conserved residues, and the conserved hydrophobic contacts (CHCs) were extracted from this data set, using both sequence and structural information. The results of this study identified a structural pattern of hydrophobic contacts shared by all of the superfamily members of fold-type I enzymes and involved in native interactions. This profile highlights the presence of a nucleus for this fold, in which residues participating in the most conserved native interactions exhibit preferential evolutionary conservation, that correlates significantly (r = 0.70) with the extent of mean hydrophobic contact value of their apolar fraction.
authors.affiliation: Dipartimento di Scienze Bio-chimiche, Università La Sapienza, Piazzale A. Moro 5, 00185 Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Paiardini
authors.forename: Francesco
authors.initials: F
authors.lastname: Bossa
authors.forename: Stefano
authors.initials: S
authors.lastname: Pascarella
mesh_terms: D017124:Conserved Sequence; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics; D019143:Evolution, Molecular; D057927:Hydrophobic and Hydrophilic Interactions; D005810:Multigene Family; D011732:Pyridoxal Phosphate / Q000737:chemistry*; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D004798:Enzymes; D011732:Pyridoxal Phosphate