B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells.
B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.
Luo
Liqun
L
Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA.
Chapoval
Andrei I
AI
Flies
Dallas B
DB
Zhu
Gefeng
G
Hirano
Fumiya
F
Wang
Shengdian
S
Lau
Julie S
JS
Dong
Haidong
H
Tamada
Koji
K
Flies
Andrew S
AS
Liu
Yang
Y
Chen
Lieping
L
D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D060887:B7 Antigens; D018122:B7-1 Antigen / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D002455:Cell Division / Q000276:immunology; D045744:Cell Line, Tumor; D002999:Clone Cells; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D018076:DNA, Complementary / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology*; D014162:Transfection
D000276:Adjuvants, Immunologic; D060887:B7 Antigens; D018122:B7-1 Antigen; C486038:Cd276 protein, mouse; D018076:DNA, Complementary; D018984:Epitopes, T-Lymphocyte