Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K.
The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.
Rahman
Raja Noor Zaliha Abd
RN
Department of Biochemistry and Microbiology, Enzyme and Microbial Technology Research, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. rnzaliha@fsas.upm.edu.my
Geok
Lee Poh
LP
Basri
Mahiran
M
Salleh
Abu Bakar
AB
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D004361:Drug Tolerance; D004789:Enzyme Activation; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D009038:Motion; D009930:Organic Chemicals / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology*; D012997:Solvents / Q000737:chemistry*; D013045:Species Specificity; D013696:Temperature
D009930:Organic Chemicals; D012997:Solvents; D010447:Peptide Hydrolases