Effect of vanillin on toxicant-induced mutation and mitotic recombination in proliferating somatic cells of Drosophila melanogaster.
Vanillin (VA; C8H8O3) is a flavoring agent that in previous studies has both increased and decreased the genotoxicity of chemical agents, depending on the nature of both the agent and the genetic event measured. The ability of VA to modulate the mutagenicity and recombinogenicity of three different monoalkylating agents, N-ethyl-N-nitrosourea (ENU), N-methyl-N-nitrosourea (MNU), and ethyl methanesulfonate (EMS), and the intercalating agent bleomycin (BLEO) was examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. While neither the mutagenicity nor the recombinagenicity of ENU or MNU was modified by posttreatment with VA, EMS-induced genetic toxicity was enhanced by as much as 30%. This overall enhancement included a synergistic increase in mitotic recombination and a lesser decrease in mutation. Posttreatment with VA also produced an increase in the genotoxicity of BLEO, which was characterized by increases of 120% and 180% for 0.5% and 1% VA, respectively. This enhancement was restricted to an increase in recombinational events, since no alteration in BLEO-induced mutation was observed. The data suggest that the major VA-modulatory action on genotoxicity in D. melanogaster is related to its synergistic effects on somatic recombination, which has a greater consequence on overall genotoxicity than its antimutagenic effects. Since the SMART assay is specifically sensitive to mitotic crossing-over, our data suggest that VA promotes toxicant-induced homologous recombination, at least in the proliferative cells of Drosophila.
Sinigaglia
Marialva
M
Laboratório de Mutagênese, Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Reguly
Maria Luíza
ML
de Andrade
Heloísa Helena Rodrigues
HH
D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001547:Benzaldehydes / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D004357:Drug Synergism; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D005421:Flavoring Agents / Q000494:pharmacology; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity; D008938:Mitosis; D009030:Mosaicism; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D011995:Recombination, Genetic
D000477:Alkylating Agents; D016587:Antimutagenic Agents; D001547:Benzaldehydes; D005421:Flavoring Agents; D008770:Methylnitrosourea; D005020:Ethyl Methanesulfonate; C100058:vanillin; D005038:Ethylnitrosourea