Identification of the luciferin-luciferase system and quantification of coelenterazine by mass spectrometry in the deep-sea luminous ostracod Conchoecia pseudodiscophora.
The bioluminescence system of the ostracod Conchoecia pseudodiscophora, which is abundant in the Sea of Japan, has been characterized. The luminescence (lambda(max)=463 nm) is produced by a luciferin-luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from C. pseudodiscophora were quantified by LC-ESI-MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine-type luciferase is present.
Oba
Yuichi
Y
Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
Tsuduki
Hiroshi
H
Kato
Shin-ichi
S
Ojika
Makoto
M
Inouye
Satoshi
S
D000818:Animals; D003445:Crustacea / Q000737:chemistry*; D049454:Firefly Luciferin / Q000737:chemistry / Q000378:metabolism*; D007093:Imidazoles / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008163:Luminescent Measurements; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D011719:Pyrazines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
D007093:Imidazoles; D011719:Pyrazines; C017144:coelenterazine; D049454:Firefly Luciferin; D008156:Luciferases