Assessing clonality of Vibrio cholerae Inaba isolates by characterization of nonsense mutations in wbeT.
The transferase gene wbeT of six clinical isolates of Vibrio cholerae O1 biotype El Tor was analysed. Two unique mutations were identified in the wbeT gene of three Inaba isolates. Due to their random nature, mutations in wbeT can be used to determine the clonal origin of clinical Inaba isolates.
Rijpkema
Sjoerd G
SG
Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK 2National Institute of Cholera and Enteric Diseases, Calcutta, India 3Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh.
Durrani
Zarmina
Z
Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK 2National Institute of Cholera and Enteric Diseases, Calcutta, India 3Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh.
Ramamurthy
T
T
Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK 2National Institute of Cholera and Enteric Diseases, Calcutta, India 3Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh.
Balakrish Nair
G
G
Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK 2National Institute of Cholera and Enteric Diseases, Calcutta, India 3Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh.
D001426:Bacterial Proteins / Q000235:genetics*; D002771:Cholera / Q000382:microbiology*; D018389:Codon, Nonsense; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004269:DNA, Bacterial; C082559:RfbT protein, Vibrio cholerae