A preliminary trial using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) on the same feedstuffs to detect tissues of animal origin.
A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues. The results of the two methods differ somewhat: PCR-RFLP did not detect any signal in any sample, but multiplex PCR detected a signal in one sample. These findings could be a basis for further investigations.
Colombo
F
F
Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy. fabio.colombo@unimi.it
Marchisio
E
E
Trezzi
I E
IE
Peri
V
V
Pinotti
L
L
Baldi
A
A
Soncini
G
G
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D008460:Meat / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine; D014422:Turkeys
D004247:DNA