title: Construction and immunogenicity of human papillomavirus type 6b L1 recombinant plasmid.
abstract: OBJECTIVE
  To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immunogenicity in mice.
  
  
  METHODS
  The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunogenicity study.
  
  
  RESULTS
  The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice's sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-gamma were increased in the same mice.
  
  
  CONCLUSIONS
  HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata.
authors.affiliation: Department of Dermatology, Beijing Chaoyang Hospital, Beijing, 100020. roseliufang@sohu.com
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jia-bi
authors.initials: JB
authors.lastname: Wang
authors.forename: Ya-gang
authors.initials: YG
authors.lastname: Zuo
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Liu
authors.forename: Dong-lai
authors.initials: DL
authors.lastname: Ma
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019556:COS Cells; D036022:Capsid Proteins; D002522:Chlorocebus aethiops; D003218:Condylomata Acuminata / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D007376:Interleukin-2; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma