title: A novel fluorescent label based on organic dye-doped silica nanoparticles for HepG liver cancer cell recognition.
abstract: In this paper, we report a method for the recognition of HepG liver cancer cells with the use of a novel fluorescent label based on organic dye-doped fluorescent silica nanoparticles. The novel organic dye-doped silica nanoparticles are prepared with a water-in-oil microemulsion technique. The silica network is produced by the controlled synchronous hydrolysis of tetraethoxysilane and 3-amino-propyltriethoxysilane (APTES). The organic dye fluorescein isothiocyanate is doped inside as a luminescent signaling element, through covalent bonding to the amino group of APTES. The organic dye-doped core-shell nanoparticles are highly luminescent and exhibit minimal dye leaching and excellent photostability. A novel fluorescent label method based on biological fluorescent nanoparticles has been developed. The dye-doped fluorescent silica nanoparticles are covalently immobilized with anti-human liver cancer monoclonal antibody HAb18. We have used antibody-labeled fluorescent nanoparticles to recognize HepG liver cancer cells. It has been observed that the bioassay based on the organic dye-doped nanoparticles can identify the target cells selectively and efficiently. The fluorescent nanoparticle label also exhibits high photostability.
authors.affiliation: National Key Laboratory of Chemical and Biological Sensing & Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.forename: Jinghua
authors.initials: J
authors.lastname: Duan
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
authors.forename: Xia
authors.initials: X
authors.lastname: Lin
authors.forename: Chunmei
authors.initials: C
authors.lastname: He
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D001681:Biological Assay; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D008027:Light; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D011437:Propylamines; D012821:Silanes / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011108:Polymers; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane