title: Antimicrobial effect of ozonated water on bacteria invading dentinal tubules.
abstract: Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. In the present study, we examined the effect of ozonated water against Enterococcus faecalis and Streptcoccus mutans infections in vitro in bovine dentin. After irrigation with ozonated water, the viability of E. faecalis and S. mutans invading dentinal tubules significantly decreased. Notably, when the specimen was irrigated with sonication, ozonated water had nearly the same antimicrobial activity as 2.5% sodium hypochlorite (NaOCl). We also compared the cytotoxicity against L-929 mouse fibroblasts between ozonated water and NaOCl. The metabolic activity of fibroblasts was high when the cells were treated with ozonated water, whereas that of fibroblasts significantly decreased when the cells were treated with 2.5% NaOCl. These results suggest that ozonated water application may be useful for endodontic therapy.
authors.affiliation: Department of Operative Dentistry and Endodontics, Kyushu Dental College, Kitakyushu, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Nagayoshi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kitamura
authors.forename: Takaki
authors.initials: T
authors.lastname: Fukuizumi
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nishihara
authors.forename: Masamichi
authors.initials: M
authors.lastname: Terashita
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology* / Q000633:toxicity; D001424:Bacterial Infections / Q000517:prevention & control; D002417:Cattle; D015169:Colony Count, Microbial; D003804:Dentin / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects; D007739:L Cells / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D010083:Oxidants, Photochemical / Q000494:pharmacology* / Q000633:toxicity; D010126:Ozone / Q000494:pharmacology* / Q000633:toxicity; D012388:Root Canal Irrigants / Q000494:pharmacology* / Q000633:toxicity; D012973:Sodium Hypochlorite / Q000494:pharmacology / Q000633:toxicity; D013295:Streptococcus mutans / Q000187:drug effects; D014867:Water
chemical_list: D000890:Anti-Infective Agents; D010083:Oxidants, Photochemical; D012388:Root Canal Irrigants; D014867:Water; D010126:Ozone; D012973:Sodium Hypochlorite