title: Intraflagellar transport motors in Caenorhabditis elegans neurons.
abstract: IFT (intraflagellar transport) assembles and maintains sensory cilia on the dendritic endings of chemosensory neurons within the nematode Caenorhabditis elegans. During IFT, macromolecular protein complexes called IFT particles (which carry ciliary precursors) are moved from the base of the sensory cilium to its distal tip by anterograde IFT motors (kinesin-II and Osm-3 kinesin) and back to the base by retrograde IFT-dynein [Rosenbaum and Witman (2002) Nat. Rev. Mol. Cell Biol. 3, 813-825; Scholey (2003) Annu. Rev. Cell Dev. Biol. 19, 423-443; and Snell, Pan and Wang (2004) Cell 117, 693-697]. In the present study, we describe the protein machinery of IFT in C. elegans, which we have analysed using time-lapse fluorescence microscopy of green fluorescent protein-fusion proteins in concert with ciliary mutants.
authors.affiliation: Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, CA 95616, USA. jmscholey@ucdavis.edu
authors.forename: J M
authors.initials: JM
authors.lastname: Scholey
authors.forename: G
authors.initials: G
authors.lastname: Ou
authors.forename: J
authors.initials: J
authors.lastname: Snow
authors.forename: A
authors.initials: A
authors.lastname: Gunnarson
mesh_terms: D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans; D002633:Chemotaxis; D002923:Cilia / Q000378:metabolism; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D008957:Models, Genetic; D046912:Multiprotein Complexes / Q000737:chemistry; D009154:Mutation; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D046912:Multiprotein Complexes; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins