title: Postoperative Corynebacterium macginleyi endophthalmitis.
abstract: A 72-year-old man with chronic endophthalmitis who received steroid treatment for 3 months came to our center. Sterile endophthalmitis after cataract extraction had been diagnosed. Aqueous samples including smears, classic cultures, and polymerase chain reaction were taken for microbiological study. Amplified DNA was sequenced to identify the pathogen. Polymerase chain reaction amplification was positive for bacteria. Sequence analysis showed Corynebacterium macginleyi as the causal agent in 48 hours. The culture and smear stains from the ocular samples were negative. The patient was successfully treated with vancomycin. Polymerase chain reaction and subsequent DNA-typing were useful in detecting the microorganisms that caused the chronic endophthalmitis.
authors.affiliation: Departamento de Biología Molecular, VISSUM, Instituto Oftalmológico de Alicante, Alicante, Spain. cferrer@oftalio.com
authors.forename: Consuelo
authors.initials: C
authors.lastname: Ferrer
authors.forename: José M
authors.initials: JM
authors.lastname: Ruiz-Moreno
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Javier
authors.initials: J
authors.lastname: Montero
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Alió
mesh_terms: D000368:Aged; D001082:Aqueous Humor / Q000382:microbiology; D015373:Bacterial Typing Techniques; D002387:Cataract Extraction; D002908:Chronic Disease; D003352:Corynebacterium / Q000235:genetics / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D004359:Drug Therapy, Combination / Q000627:therapeutic use; D009877:Endophthalmitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D005784:Gene Amplification; D006801:Humans; D019654:Lens Implantation, Intraocular; D008297:Male; D015242:Ofloxacin / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications; D014031:Tobramycin / Q000627:therapeutic use; D014640:Vancomycin / Q000627:therapeutic use; D014822:Vitreous Body / Q000382:microbiology
chemical_list: D014640:Vancomycin; D015242:Ofloxacin; D014031:Tobramycin