title: A preliminary trial using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) on the same feedstuffs to detect tissues of animal origin.
abstract: A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues. The results of the two methods differ somewhat: PCR-RFLP did not detect any signal in any sample, but multiplex PCR detected a signal in one sample. These findings could be a basis for further investigations.
authors.affiliation: Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Milan, Italy. fabio.colombo@unimi.it
authors.forename: F
authors.initials: F
authors.lastname: Colombo
authors.forename: E
authors.initials: E
authors.lastname: Marchisio
authors.forename: I E
authors.initials: IE
authors.lastname: Trezzi
authors.forename: V
authors.initials: V
authors.lastname: Peri
authors.forename: L
authors.initials: L
authors.lastname: Pinotti
authors.forename: A
authors.initials: A
authors.lastname: Baldi
authors.forename: G
authors.initials: G
authors.lastname: Soncini
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D008460:Meat / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine; D014422:Turkeys
chemical_list: D004247:DNA