Expression pattern of FCRL (FREB, FcRX) in normal and neoplastic human B cells.
FCRL (also known as FREB and FcRX) is a recently described member of the family of Fc receptors for immunoglobulin G (IgG). In the present study we analysed its expression in normal and neoplastic lymphoid tissue using immunohistochemical techniques. FCRL was preferentially expressed in a proportion of germinal centre cells and, more weakly, in mantle zone B cells. In addition, strong labelling was observed in marginal zone B cells in the spleen, representing one of the few markers for this cell type. The majority of cases of small B-cell lymphoma, diffuse large B-cell lymphoma and lymphocyte predominance Hodgkin's disease were positive for FCRL. However, the number of positive cells varied widely, and in consequence we could not define a cut-off that distinguished subsets of diffuse large B-cell lymphoma. Our results also showed that FCRL tended to be negative in T-cell-rich B-cell lymphoma and in classical Hodgkin's disease. FCRL may therefore represent a novel marker for normal B cells (e.g. splenic marginal zone cells) and may also be useful as a potential marker of B-cell neoplasms.
Masir
Noraidah
N
Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, UK.
Jones
Margaret
M
Pozzobon
Michela
M
Marafioti
Teresa
T
Volkova
Olga Y
OY
Mechetina
Ludmila V
LV
Hansmann
Martin-Leo
ML
Natkunam
Yasodha
Y
Taranin
Alexander V
AV
Mason
David Y
DY
D001402:B-Lymphocytes / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D006689:Hodgkin Disease / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016393:Lymphoma, B-Cell / Q000737:chemistry*; D011961:Receptors, Fc / Q000032:analysis*
D015415:Biomarkers; C452186:FCRLA protein, human; D011961:Receptors, Fc