title: Immunization with modified vaccinia virus Ankara-based recombinant vaccine against severe acute respiratory syndrome is associated with enhanced hepatitis in ferrets.
abstract: Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) is a serious emerging human infectious disease. In this report, we immunized ferrets (Mustela putorius furo) with recombinant modified vaccinia virus Ankara (rMVA) expressing the SARS-CoV spike (S) protein. Immunized ferrets developed a more rapid and vigorous neutralizing antibody response than control animals after challenge with SARS-CoV; however, they also exhibited strong inflammatory responses in liver tissue. Inflammation in control animals exposed to SARS-CoV was relatively mild. Thus, our data suggest that vaccination with rMVA expressing SARS-CoV S protein is associated with enhanced hepatitis.
authors.affiliation: National Centre for Foreign Animal Diseases, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2.
authors.forename: Hana
authors.initials: H
authors.lastname: Weingartl
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
authors.forename: Stefanie
authors.initials: S
authors.lastname: Czub
authors.forename: James
authors.initials: J
authors.lastname: Neufeld
authors.forename: Peter
authors.initials: P
authors.lastname: Marszal
authors.forename: Jason
authors.initials: J
authors.lastname: Gren
authors.forename: Greg
authors.initials: G
authors.lastname: Smith
authors.forename: Shane
authors.initials: S
authors.lastname: Jones
authors.forename: Roxanne
authors.initials: R
authors.lastname: Proulx
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Deschambault
authors.forename: Elsie
authors.initials: E
authors.lastname: Grudeski
authors.forename: Anton
authors.initials: A
authors.lastname: Andonov
authors.forename: Runtao
authors.initials: R
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: John
authors.initials: J
authors.lastname: Copps
authors.forename: Allen
authors.initials: A
authors.lastname: Grolla
authors.forename: Daryl
authors.initials: D
authors.lastname: Dick
authors.forename: Jody
authors.initials: J
authors.lastname: Berry
authors.forename: Shelley
authors.initials: S
authors.lastname: Ganske
authors.forename: Lisa
authors.initials: L
authors.lastname: Manning
authors.forename: Jingxin
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005289:Ferrets; D006505:Hepatitis / Q000209:etiology*; D008099:Liver / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D064370:Spike Glycoprotein, Coronavirus; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000009:adverse effects*; D014616:Vaccinia virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000009:adverse effects*
chemical_list: D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus