title: Reviewing the mechanism of action of thiopurine drugs: towards a new paradigm in clinical practice.
abstract: The precise mechanism of action of thiopurine drugs remains unclear despite more than 40 years of use. Recent knowledge in the field of apoptosis and a better insight into, as well as a rapid increase in their use in several important areas of clinical medicine justify this appraisal. This is a review of the recent advances in the knowledge of their mechanism of action and is primarily intended to help clinicians understand the pharmacological properties of these drugs adequately and to find ways to improve their use in clinical practice. The parent compound is azathioprine (AZA), which is rapidly reduced in the presence of glutathione to 6-mercaptopurine (6-MP) and then metabolized into active metabolites with immune-modifier activity. Recent observations and new data indicate that AZA/6-MP could be considered as a "two-in-one" drug, providing a source of 6-thioguanine nucleotides (6-TGNs) and methylated metabolites, and that both compounds could contribute to its antiproliferative effects. This review will also focus on mechanisms that may help to explain a number of recent observations showing that myelotoxicity may occur in patients with high TPMT level or low 6-TGN rate. Our final proposal suggests that the immunosuppressive effects of these drugs are due to a balanced combination of antimetabolic and pro-apoptotic actions.
authors.affiliation: Scientific Department, Celltech Pharma, Madrid, Spain. carlos.cara@celltechgroup.com
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Cara
authors.forename: Amado Salvador
authors.initials: AS
authors.lastname: Pena
authors.forename: Miquel
authors.initials: M
authors.lastname: Sans
authors.forename: Luis
authors.initials: L
authors.lastname: Rodrigo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Guerrero-Esteo
authors.forename: Joaquín
authors.initials: J
authors.lastname: Hinojosa
authors.forename: Julio
authors.initials: J
authors.lastname: García-Paredes
authors.forename: Luis G
authors.initials: LG
authors.lastname: Guijarro
mesh_terms: D017209:Apoptosis; D006801:Humans; D007166:Immunosuppressive Agents / Q000378:metabolism / Q000494:pharmacology*; D011687:Purines / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D013457:Sulfur Compounds / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D007166:Immunosuppressive Agents; D011687:Purines; D013457:Sulfur Compounds