title: [Expression and significance of caspase-3 gene in apoptotic muscle cells 103Pd radioactive stent bile duct in dogs].
abstract: OBJECTIVE
  To discuss the expression and significance of caspase-3 gene in the apoptotic muscle cells in gamma-radiation-induced muscle cell lines.
  
  
  METHODS
  The caspase-3 mRNA in the control and gamma-radiation induced apoptotic muscle cells was analysed by RT-PCR.
  
  
  RESULTS
  The expression of caspase-3 gene transcript was higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct, and apoptotic muscle cells were higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct.
  
  
  CONCLUSIONS
  The high level expression of caspase-3 gene may help to understand the muscle cells sensitivity to gamma-radiation apoptosis. 103Pd radioactive stent may increase the expression of caspase-3 gene in dog bile duct and prevent the billiary narrow when dog bile duct was injured by balloon.
authors.affiliation: Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China.
authors.forename: Gui-Jin
authors.initials: GJ
authors.lastname: He
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
authors.forename: Qin-Yi
authors.initials: QY
authors.lastname: Gao
authors.forename: Shu-He
authors.initials: SH
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Dai
authors.forename: Kai
authors.initials: K
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D051017:Apoptosis Regulatory Proteins; D001652:Bile Ducts / Q000201:enzymology / Q000528:radiation effects*; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000528:radiation effects*; D004285:Dogs; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000528:radiation effects*; D010165:Palladium / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000235:genetics / Q000528:radiation effects; D011868:Radioisotopes / Q000008:administration & dosage*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015607:Stents
chemical_list: D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger; D011868:Radioisotopes; D010165:Palladium; D053148:Caspase 3; D020169:Caspases