[Quantification of isopimpinellin in root of Toddalia asiatica by HPLC].
OBJECTIVE
To determine the content of isopimpinellin in root of Toddalia asiatica.


METHODS
A HPLC method was set up. Using Hypersil C18 column and methanol-water (70:30) as mobile phase, with the detection wavelength at 306 nm.


RESULTS
The linear range of isopimpinellin was 0.004 20 approximately 0.420 microg. The average recovery was 99.7% and the RSD 2.8%.


CONCLUSIONS
The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.
Hao
Xiao-Yan
XY
Department of Pharmacy, Guiyang Medical College, Guiyang 550004, China. haoxiaoyan@vip.163.com
Cao
Xiao-Hong
XH
Liang
Yan
Y
Shen
Yue-Mao
YM
D002851:Chromatography, High Pressure Liquid; D011564:Furocoumarins / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D027821:Rutaceae / Q000737:chemistry*
D011564:Furocoumarins; C015304:isopimpinellin