Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry.
An analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation of Corynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation of C. glutamicum to different growth conditions is demonstrated.
Strelkov
Sergey
S
Institute of Biochemistry, University of Cologne, Zülpicher Str. 47, D-50674 Cologne, Germany.
von Elstermann
Mirko
M
Schomburg
Dietmar
D
D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods