title: Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibles.
abstract: OBJECTIVE
  The aim of this work was to evaluate the effectiveness of homogenous demineralized dentin matrix (HDDM) slices in surgical bone defects created in the mandibles of rabbits and occluded with a polytetrafluoroethylene (PTFE) membrane in the promotion of bone growth.
  
  
  METHODS
  Surgical bone defects were created in 36 adult rabbits and divided into 4 groups: bone defect (control), bone defect with PTFE membrane, bone defect with HDDM, and bone defect with both HDDM and a PTFE membrane (HDDM + PTFE). The rabbits were sacrificed after 30, 60, and 90 days, and the bone defects were examined histologically and by histomorphometric analysis (analysis of variance and the Tukey test).
  
  
  RESULTS
  The volume of newly formed bone matrix was significantly greater in the HDDM and HDDM + PTFE groups than in the control and PTFE groups. The discrete inflammatory reaction found in the HDDM and HDDM + PTFE groups did not prevent the osteopromotive activity of the dentin matrix.
  
  
  CONCLUSIONS
  HDDM slices were biocompatible and were resorbed during the bone remodeling process. They stimulated the newly formed bone until 30 days after implantation.
  
  
  CONCLUSIONS
  Bone repair was accelerated in the bone defects treated with HDDM in comparison to the control group.
authors.affiliation: Department of Biosciences and Oral Diagnosis, São José dos Campos School of Dentistry, São Paulo State University (UNESP), São Jose dos Campos, São Paulo, Brazil. valcarvalho@directnet.com.br
authors.forename: Valéria Abrantes Pinheiro
authors.initials: VA
authors.lastname: Carvalho
authors.forename: Darcy de Oliveira
authors.initials: Dde O
authors.lastname: Tosello
authors.forename: Miguel Angel de Castillo
authors.initials: MA
authors.lastname: Salgado
authors.forename: Mônica Fernandes
authors.initials: MF
authors.lastname: Gomes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D016723:Bone Remodeling / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use; D003764:Dental Materials / Q000627:therapeutic use*; D003804:Dentin / Q000637:transplantation; D048091:Guided Tissue Regeneration; D006253:Haversian System / Q000187:drug effects / Q000473:pathology; D008336:Mandibular Diseases / Q000473:pathology / Q000601:surgery*; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000187:drug effects*; D011138:Polytetrafluoroethylene; D011817:Rabbits; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; C093073:Demineralized Dentin Matrix; D003764:Dental Materials; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene