An assay of mammalian cell micropermeabilization based on measurements of cellular lactate production.
A method for the quantitative assay of mammalian cell micropermeabilization is described. The method is based on the permeabilization-induced loss of endogenous glycolytic cofactors and consequent discontinuation of cellular lactate production. Advantages of the method include sensitivity and precision similar to that of micropermeabilization assays based on the release of 86Rb+ from preloaded cells, avoidance of radioactivity, and simplicity of the measurements and equipment required.
Lehoux
Eric A
EA
Department of Biochemistry and Molecular Biology, Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA. elehoux@biochem.okstate.edu
Baker
Sharon M
SM
Bush
Jeremy A
JA
Spivey
H Olin
HO
D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D006019:Glycolysis; D019344:Lactic Acid / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D010758:Phosphorus / Q000494:pharmacology; D051381:Rats; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000378:metabolism
D001427:Bacterial Toxins; D010758:Phosphorus; D019344:Lactic Acid