title: Catching protein antigens by antibody affinity electrophoresis.
abstract: A new kind of affinity electrophoresis called antibody affinity electrophoresis is a technique used to capture protein antigens based on their interactions with specific monoclonal or polyclonal antibodies incorporated in the polyacrylamide gel. Polyclonal anti-glutathione-S-transferase (anti-GST), monoclonal anti-bovine serum albumin (anti-BSA), and polyclonal anti-human alpha-lactalbumin are embedded in distinct areas of a 7.5% native polyacrylamide gel. Some of the embedded antibodies get covalently and/or noncovalently incorporated into the gel matrix network. Under electrophoresis conditions, these antibodies do not show significant electrophoretic mobility, as compared to their specific protein antigen analytes. We observed that electrophoretic migration of GST, BSA, and protein G ceases when they encounter anti-GST, anti-BSA, and immunoglobulin G, respectively.
authors.affiliation: Protein Research Laboratory, Research Resources Center, University of Illinois, Chicago, IL, USA. boblee@uic.edu
authors.forename: Bao-Shiang
authors.initials: BS
authors.lastname: Lee
authors.forename: Shalini
authors.initials: S
authors.lastname: Gupta
authors.forename: Sangeeth
authors.initials: S
authors.lastname: Krisnanchettier
authors.forename: Syed S
authors.initials: SS
authors.lastname: Lateef
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D000915:Antibody Affinity; D000941:Antigens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D000941:Antigens; D011506:Proteins; C016680:polyacrylamide gels; D014357:Trypsin