Effect of Polyscias filicifolia Bailey biomass on protein synthesis process in isolated pig heart.
OBJECTIVE
The aim of this study was to investigate effect of Polyscias filicifolia Bailey biomass on protein synthesis process in normoxic and anoxic pig heart.


METHODS
Experiments were done on isolated pig hearts weighing 100-150 g. Effects of anoxia were evaluated after 20 and 90 min of anoxic perfusion. Control hearts were perfused under aerobic conditions. Investigating action of Polyscias filicifolia Bailey, pig heart was perfused under normoxic and anoxic condition with buffer, which contains tincture Polyscias filicifolia Bailey. For the determination of protein synthesis rate (time of incubation was 15 min) and level (time of incubation was 60 min), incorporation of [14C]-leucine into translational products in a cell-free system was measured.


RESULTS
Protein synthesis rate and level in cell-free system decreased by 30% and 20% respectively after 20 min anoxic perfusion and by 48% and 45% respectively after 90 min anoxic perfusion in comparison to the control. Polyscias filicifolia Bailey tincture did not affect the protein synthesis rate and level in cell-free translation system from control pig heart. Polyscias filicifolia Bailey tincture has protective effect on protein synthesis system from pig hearts after 20 min and 90 min anoxic perfusion. Protein synthesis rate and level after 20 min anoxic perfusion with Polyscias filicifolia Bailey tincture was the same as at a control level. However, after 90 min anoxic perfusion with Polyscias filicifolia Bailey tincture protein synthesis rate and level did not reach control level and represented 81% and 76% respectively of control values. Spectrum of newly synthesized polypeptides in cell-free protein synthesis system under anoxic conditions and after treatment with Polyscias filicifolia Bailey tincture did not differ.


CONCLUSIONS
Protein synthesis rate and level decreased under long and short-term anoxia. Polyscias filicifolia Bailey biomass restore protein synthesis system under anoxia. Neither anoxia, nor Polyscias filicifolia Bailey tincture did not influence spectrum of newly synthesized proteins.
Kasauskas
Artūras
A
Department of Biochemistry, Kaunas University of Medicine, A. Mickeviciaus 9, 44307 Kaunas, Lithuania. akasauskas@one.lt
Viezeliene
Dale
D
D000818:Animals; D027481:Araliaceae; D001345:Autoradiography; D018533:Biomass; D002021:Buffers; D002474:Cell-Free System / Q000378:metabolism; D003627:Data Interpretation, Statistical; D004591:Electrophoresis, Polyacrylamide Gel; D000860:Hypoxia / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D028321:Plant Preparations / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012996:Solutions; D013552:Swine; D013997:Time Factors
D002021:Buffers; D028321:Plant Preparations; D012996:Solutions