title: Identification and distribution of new insertion sequences in the genome of the extremely halotolerant and alkaliphilic Oceanobacillus iheyensis HTE831.
abstract: Six kinds of new insertion sequences (ISs), IS667 to IS672, a group II intron (Oi.Int), and an incomplete transposon (Tn852loi) were identified in the 3,630,528-bp genome of the extremely halotolerant and alkaliphilic Oceanobacillus iheyensis HTE831. Of 19 ISs identified in the HTE831 genome, 7 were truncated, indicating the occurrence of internal rearrangement of the genome. All ISs except IS669 generated a 4- to 8-bp duplication of the target site sequence, and these ISs carried 23- to 28-bp inverted repeats (IRs). Sequence analysis revealed that four ISs (IS669, IS670, IS671, and IS672) were newly identified as belonging to separate IS families (IS200/IS605, IS30, IS5, and IS3, respectively). IS667 and IS668 were also characterized as new members of the ISL3 family. Tn8521oi, which belongs to the Tn3 family as a new member, generated a 5-bp duplication of the target site sequence and carried complete 38-bp IRs. Of the eight protein-coding sequences (CDSs) identified in Tn8521oi, three CDSs (OB481, OB482, and OB483) formed a ger gene cluster, and two other paralogous gene clusters were found in the HTE831 genome. Most of the ISs and the group II intron widely distributed throughout the genome were inserted in noncoding regions, while two ISs (IS667-08 and IS668-02) and Oi.Int-04 were inserted in the coding regions.
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Matsuki
authors.forename: Gab-Joo
authors.initials: GJ
authors.lastname: Chee
authors.forename: Hideto
authors.initials: H
authors.lastname: Takami
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000145:classification / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D020440:Gene Duplication; D016680:Genome, Bacterial; D016931:Halobacteriales / Q000235:genetics* / Q000502:physiology; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics; D016337:RNA, Catalytic / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D013171:Spores, Bacterial; D019895:Transposases / Q000235:genetics; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D019895:Transposases; D012194:RNA-Directed DNA Polymerase