An angiotensin II AT1 receptor antagonist, telmisartan augments glucose uptake and GLUT4 protein expression in 3T3-L1 adipocytes.
Evidence has accumulated that some of the angiotensin II AT1 receptor antagonists have insulin-sensitizing property. We thus examined the effect of telmisartan on insulin action using 3T3-L1 adipocytes. With standard differentiation inducers, a higher dose of telmisartan effectively facilitated differentiation of 3T3-L1 preadipocytes. Treatment of both differentiating adipocytes and fully differentiated adipocytes with telmisartan caused a dose-dependent increase in mRNA levels for PPARgamma target genes such as aP2 and adiponectin. By contrast, telmisartan attenuated 11beta-hydroxysteroid dehydrogenase type 1 mRNA level in differentiated adipocytes. Of note, we demonstrated for the first time that telmisartan augmented GLUT4 protein expression and 2-deoxy glucose uptake both in basal and insulin-stimulated state of adipocytes, which may contribute, at least partly, to its insulin-sensitizing ability.
Fujimoto
Muneya
M
Division of Endocrinology and Metabolism, Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54, Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Masuzaki
Hiroaki
H
Tanaka
Tomohiro
T
Yasue
Shintaro
S
Tomita
Tsutomu
T
Okazawa
Kayoko
K
Fujikura
Junji
J
Chusho
Hideki
H
Ebihara
Ken
K
Hayashi
Tatsuya
T
Hosoda
Kiminori
K
Nakao
Kazuwa
K
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000235:genetics*; D009124:Muscle Proteins / Q000187:drug effects / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000187:drug effects*; D000077333:Telmisartan
D000806:Angiotensin-Converting Enzyme Inhibitors; D001562:Benzimidazoles; D001565:Benzoates; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D044140:Receptor, Angiotensin, Type 1; C495693:Slc2a4 protein, mouse; D003847:Deoxyglucose; D005947:Glucose; D000077333:Telmisartan