title: Extracellularly regulated kinases 1/2 (p44/42 mitogen-activated protein kinases) phosphorylate synapsin I and regulate insulin secretion in the MIN6 beta-cell line and islets of Langerhans.
abstract: The p44/p42 MAPKs (ERK1/2) cascade regulates beta-cell nuclear events, which modulates cell differentiation and gene transcription, whereas its implication in processes occurring in the cytoplasm, such as activation of the exocytotic machinery, is still unclear. Using the MIN6 beta-cell line and isolated rat islets of Langerhans, we investigated whether glucose, by activating the ERK1/2 cascade, induces phosphorylation of cytoplasmic proteins implicated in exocytosis of insulin granules such as synapsin I. We observed that the majority of ERK1/2 activity induced by glucose remains in the cytoplasm and physically interacts with synapsin I, allowing phosphorylation of the substrate. Therefore, we reexamined the potential requirement of ERK1/2 for insulin secretion. Blocking activation of ERK1/2 using MEK1/2, the MAPK kinase inhibitor PD98059 or using small interfering RNA-mediated silencing of ERK1 and ERK2 expressions resulted in partial inhibition of glucose-induced insulin release, indicating that ERK1/2 pathway participates also in the regulation of insulin secretion. Moreover, using the pancreatic islet perifusion model, we found that the ERK1/2 activity participates in the first and second phases of insulin release induced by glucose. Taken together, our results demonstrate new aspects of the glucose-dependent actions of ERK1/2 in beta-cells exerted on cytoplasmic proteins, including synapsin I, and participating in the overall glucose-induced insulin secretion.
authors.affiliation: Unité Institut National de la Santé et de la Recherche Médecale U376, Centre Hospitalier Universitaire Arnaud de Villeneuve, 371 Rue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Longuet
authors.forename: Christophe
authors.initials: C
authors.lastname: Broca
authors.forename: Safia
authors.initials: S
authors.lastname: Costes
authors.forename: El Habib
authors.initials: EH
authors.lastname: Hani
authors.forename: Dominique
authors.initials: D
authors.lastname: Bataille
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dalle
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms; D010766:Phosphorylation / Q000187:drug effects; D034741:RNA, Small Interfering; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000378:metabolism*
chemical_list: D007328:Insulin; D034741:RNA, Small Interfering; D016704:Synapsins; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D005947:Glucose