title: Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray.
abstract: The complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. To understand these molecular mechanisms, we performed cDNA microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. Microarray analysis of 4,304 cDNA clones revealed that the hybridization signal of 396 cDNA clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of leaves. Cluster analysis with the expression data showed that 259 cDNA clones (156 non redundant groups) were specifically or predominantly expressed in anther tissues and were regulated by developmental stage-specific manners in the anther tissues. These co-regulated genes would be important for development of functional anther tissues. Furthermore, we selected several clones for RNA in situ hybridization analysis. From these analyses, we found several novel genes that show temporal and spatial expression patterns during anther development in addition to anther-specific genes reported so far. These results indicate that the genes identified in this experiment are controlled by different programs and are specialized in their developmental and cell types.
authors.affiliation: Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Endo
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saito
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsubara
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hakozaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko
authors.forename: Motoshi
authors.initials: M
authors.lastname: Kamada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Higashitani
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
authors.forename: Taku
authors.initials: T
authors.lastname: Demura
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics*; D035264:Flowers / Q000378:metabolism*; D005456:Fluorescent Dyes; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D005456:Fluorescent Dyes