Inhibitory effect of water-soluble chitosan on TNF-alpha and IL-8 secretion from HMC-1 cells.
Mast cells are known to play an active role as effector cells in allergic inflammation and in diverse immunological and pathological processes. Activated mast cell-derived pro-inflammatory cytokines are important pathologic factors of progression of allergic inflammation. In this study, we investigated whether pro-inflammatory cytokines (TNF-alpha and IL-8) can be induced by calcium stimulation in HMC-1 cells, and high molecular weight water-soluble chitosan (WSC) can inhibit the production of these cytokines. We provided evidence that the secretion of TNF-alpha and IL-8 from HMC-1 cells was induced by Ca2+-ionophore A23187 or Ca2+-ATPase inhibitor TSG. Treatment of WSC (10 microg/ml) prior to stimulation with calcium agonists significantly blocked the secretion of TNF-alpha by 65.1% for A23187 and 87.7% for TSG. IL-8 secretion in response to A23187 or TSG was inhibited by 49.2% for A23187 and 34.1% for TSG, respectively, compared to absence of WSC. These results suggest that WSC has potential regulatory effects on allergic inflammatory diseases by down-modulating Ca2+-induced mast cell activation.
Kim
Mi-Sun
MS
College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.
You
Hyun Ja
HJ
You
Mi Kyung
MK
Kim
Nam-Song
NS
Shim
Bum Sang
BS
Kim
Hyung-Min
HM
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000819:agonists / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D048271:Chitosan / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D012995:Solubility; D019284:Thapsigargin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism*; D014867:Water
D004791:Enzyme Inhibitors; D016209:Interleukin-8; D007476:Ionophores; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D000001:Calcimycin; D019284:Thapsigargin; D048271:Chitosan; D000252:Calcium-Transporting ATPases; D002118:Calcium