title: Crystallization and preliminary diffraction studies of TraF, a component of the Escherichia coli type IV secretory system.
abstract: TraF, a component of the Escherichia coli type IV secretory system, has been crystallized and preliminary X-ray diffraction data have been collected. TraF is a 26 kDa protein encoded by the E. coli F plasmid and is required for conjugative plasmid transfer and the formation of sex pili. The N-terminal domain of TraF has no recognizable sequence features, whereas the C-terminal domain is believed to adopt a thioredoxin fold. However, since the active-site cysteines of thioredoxin-like proteins are not conserved in TraF, its biochemical role remains unclear. TraF crystallizes in space group C2, with unit-cell parameters a = 119.87, b = 34.36, c = 46.21 A, beta = 90.40 degrees , and crystals diffract to 2.3 A resolution.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Audette
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Holland
authors.forename: Trevor C
authors.initials: TC
authors.lastname: Elton
authors.forename: Jan
authors.initials: J
authors.lastname: Manchak
authors.forename: Koto
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Laura S
authors.initials: LS
authors.lastname: Frost
authors.forename: Bart
authors.initials: B
authors.lastname: Hazes
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C500309:TraF protein, E coli