[Study on the potentiation of bone marrow mesenchymal stem cells involved in sebaceous duct formation].
OBJECTIVE
To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into skin appandence.


METHODS
Porcine MSCs were isolated from porcine marrow and grown in vitro. After labeling with BrdU, MSCs were engrafted to porcine skin. At 1, 2, 4 weeks after the transplantation, immunohistochemical examinations were carried out to detect the positive staining of BrdU and cytokeratin.


RESULTS
A few sebaceous duct cells, which expressed cytokeratin, were also BrdU positive, and these cells were considered may to be transplanted MSCs-derived cells.


CONCLUSIONS
Porcine MSCs might have the potential to differentiated into sebaceous duct cells in skin.
Fang
Li-jun
LJ
Department of Stomatology of the Second Affiliated Hospital of Chinese Medical University, Shenyang 110003, China. fuxb@vip.sina.com
Fu
Xiao-bing
XB
Cheng
Biao
B
Sun
Tong-zhu
TZ
Li
Jian-fu
JF
Cao
Rong
R
Wang
Yu-xin
YX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012627:Sebaceous Glands / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous