Novel application of sRNA: stimulation of ribosomal frameshifting.
Small RNAs play an important role in regulation of gene expression in eukaryotic and eubacterial cells by modulating gene expression both at the level of transcription and translation. Here, we show that short complementary RNAs can also affect gene expression by stimulating ribosomal frameshifting in vitro. This finding has important implications for understanding the process of ribosomal frameshifting and for the potential application of small RNAs in the treatment of diseases that are due to frameshift mutations.
Olsthoorn
R C L
RC
Leiden Institute of Chemistry, Section Genexpress, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, P.O. Box 9502, 2300RA Leiden, The Netherlands. olsthoor@chem.leidenuniv.nl
Laurs
M
M
Sohet
F
F
Hilbers
C W
CW
Heus
H A
HA
Pleij
C W A
CW
D020029:Base Pairing; D003062:Codon; D018388:Codon, Terminator; D004742:Enhancer Elements, Genetic; D018965:Frameshifting, Ribosomal / Q000235:genetics*; D005786:Gene Expression Regulation; D009841:Oligonucleotides / Q000378:metabolism; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D013816:Thermodynamics; D014158:Transcription, Genetic
D003062:Codon; D018388:Codon, Terminator; D009841:Oligonucleotides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins