title: Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I.
abstract: Two crystal forms, rhombohedral and hexagonal, of a homing endonuclease from Thermoproteus sp. IC-061 (I-Tsp0611) were obtained by the hanging-drop and sitting-drop method, respectively. The hexagonal crystals belong to space group P6(3)22, with unit-cell parameters a = b = 111.4, c = 97.6 A, and diffract to 3.2 A resolution on beamline BL44 at SPring-8 (Harima, Japan). The rhombohedral crystals belong to space group R32, with unit-cell parameters a = b = 95.4, c = 192.9 A, and diffract to 2.7 A resolution using a Cu Kalpha rotating-anode generator with an R-AXIS VII detector. The crystal asymmetric unit contained one protein molecule and the solvent contents of the two crystal forms were estimated to be 68.3 and 67.6% by volume, respectively.
authors.affiliation: Institute for Health Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Imagawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakayama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Katunuma
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sako
authors.forename: Norimichi
authors.initials: N
authors.lastname: Nomura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsuge
mesh_terms: D003460:Crystallization; D003850:Deoxyribonuclease I / Q000737:chemistry*; D041542:Thermoproteus / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D003850:Deoxyribonuclease I