Importance of structural tightening, as opposed to partially bound States, in the determination of chemical shift changes at noncovalently bonded interfaces.
Two models (A and B) have been proposed to account for decreased downfield chemical shifts of a proton bound by noncovalent interactions at a ligand/antibiotic interface as the number of ligand/antibiotic interactions is decreased. In model A, the proton involved in the noncovalent bond suffers a smaller downfield shift because the bond is, with a relatively large probability, broken, and not because it is longer. In model B, the proton involved in the noncovalent bond suffers a smaller downfield shift because the bond is longer, and not because it is, with a relatively large probability, broken. We show that model A cannot account for the chemical shift changes. Model B accounts for the process of positively cooperative binding, in which noncovalent bonds are reduced in length and thereby increase the stability of the organized state.
Williams
Dudley H
DH
Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW, UK.
Davies
Nichola L
NL
Koivisto
Jari J
JJ
D001665:Binding Sites; D006860:Hydrogen Bonding; D007700:Kinetics; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D012310:Ristocetin / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism
D009842:Oligopeptides; C112142:chloroeremomycin; D012310:Ristocetin; C016719:ristocetin A; C043367:lysyl-alanyl-alanine; D014640:Vancomycin