Abnormal migration of human wild-type alpha-synuclein upon gel electrophoresis.
Alpha-synuclein aggregates have been linked to the pathogenesis of Parkinson's disease (PD), with Lewy bodies (LBs) and Lewy neurites (LNs) constituting the pathological hallmarks in the brains of patients with PD and dementia with LBs. LBs are formed by the conversion of soluble monomers of alpha-synuclein into insoluble aggregates. Here we report an abnormal electrophoretic mobility, at a higher molecular weight (MW) than the expected theoretical MW, of both recombinant histidine-tagged human alpha-synuclein, human alpha-synuclein expressed in SH-SY5Y human neuroblastoma cells or Ltk(-) fibroblasts, and rat brain alpha-synuclein, on SDS-PAGE polyacrylamide, but not on Nu-PAGE gradient peptide, gels, suggesting possible alpha-synuclein data misinterpretations associated with gel electrophoresis. These studies raise important considerations about the type of protein gel electrophoresis system suitable to study the alterations of alpha-synuclein associated with neurodegeneration, PD and other synucleinopathies.
Moussa
Charbel E-H
CE
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA.
Wersinger
Christophe
C
Rusnak
Milan
M
Tomita
York
Y
Sidhu
Anita
A
D000818:Animals; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D051843:Synucleins; D051844:alpha-Synuclein
D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C497604:SNCA protein, human; C497606:Snca protein, rat; D051843:Synucleins; D051844:alpha-Synuclein