LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA.
Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double-stranded DNA. Structural studies have shown that LNA oligonucleotides induce A-type (RNA-like) duplex conformations. The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.
Vester
Birte
B
Nucleic Acid Center, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense. b.vester@bmb.sdu.dk
Wengel
Jesper
J
D018076:DNA, Complementary / Q000737:chemistry* / Q000378:metabolism; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D018716:Molecular Mimicry; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D018075:RNA, Complementary / Q000737:chemistry* / Q000378:metabolism; D012266:Ribose / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use
C434798:2'-O-4'-C-methylene-ribofuranosyl nucleotide; D018076:DNA, Complementary; D009711:Nucleotides; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D018075:RNA, Complementary; C477371:locked nucleic acid; D012266:Ribose; D004722:Endoribonucleases