Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing.
An siRNA nanocarrier formed through self-assembly of PEG-based block catiomer possessing two distinct amino groups with different pKa values in a side chain was developed. This design provided the carrier with a sufficient siRNA complexation and an assumed buffering capacity in the endosomes, allowing it to exhibit remarkable gene knockdown abilities as well as sufficient serum tolerability.
Itaka
Keiji
K
Department of Materials Science and Engineering, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Kanayama
Naoki
N
Nishiyama
Nobuhiro
N
Jang
Woo-Dong
WD
Yamasaki
Yuichi
Y
Nakamura
Kozo
K
Kawaguchi
Hiroshi
H
Kataoka
Kazunori
K
D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics; D003959:Diamines / Q000737:chemistry*; D020868:Gene Silencing; D006801:Humans; D007700:Kinetics; D049329:Nanostructures / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics
D002412:Cations; D003959:Diamines; D010455:Peptides; D034741:RNA, Small Interfering; C033141:poly-beta-benzyl-aspartate; D011092:Polyethylene Glycols; D004247:DNA