Plasma protein adsorption to surfaces grafted with dense homopolymer and copolymer brushes containing poly(N-isopropylacrylamide).
Growing polymer chains from surface initiators in principle allows much more dense polymer surface layers to be created than can be produced by grafting of whole (self-excluding) chains. We have utilized aqueous atom transfer radical polymerization to graft a series of cleavable hydrophilic poly(N-isopropylacrylamide) (PNIPAM) homopolymers and block copolymers of substituted acrylamides from polystyrene latex to give brushes of controlled MW and surface density. Average chain separations much less than their free solution radii of gyration have been achieved. Exposure to radiolabeled single proteins or to whole plasma and subsequent analysis by SDS-PAGE shows that PNIPAM brushes decrease protein adsorption relative to the latex surface or other substituted polyacrylamides. The PNIPAM brushes exhibit a second-order phase transition around 30 degrees C as reflected by a decrease in the hydrodynamic thickness of the brush at higher temperatures. Total plasma protein adsorption is increased at 40 degrees C compared to 20 degrees C but there is significant differential adsorption behavior among the proteins detected by gel-electrophoresis analysis.
Janzen
Johan
J
Department of Pathology and Laboratory Medicine, 2211 Wesbrook Mall, University of British Columbia, Vancouver, BC V6T 2B5, Canada.
Le
Yevgeniya
Y
Kizhakkedathu
Jayachandran N
JN
Brooks
Donald E
DE
D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D000327:Adsorption; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007840:Latex / Q000737:chemistry; D008422:Materials Testing; D008970:Molecular Weight; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D013816:Thermodynamics
D000180:Acrylic Resins; D001672:Biocompatible Materials; D001798:Blood Proteins; D007840:Latex; D012709:Serum Albumin; C052970:poly-N-isopropylacrylamide