Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates.
Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.
Hong
Rui
R
Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.
Emrick
Todd
T
Rotello
Vincent M
VM
D000085:Acetates / Q000737:chemistry*; D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000085:Acetates; D005026:Ethylene Glycols; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; D006046:Gold; D002918:Chymotrypsin