Biocompatible glucose sensor prepared by modifying protein and vinylferrocene monomer composite membrane.
This paper proposes a very simple procedure for preparing a biocompatible sensor based on a protein (bovine serum albumin, BSA), enzyme and vinylferrocene (VF) composite membrane modified electrode. The membrane was prepared simply by first casting vinylferrocene and then coating it with BSA and glucose oxidase immobilised with glutaraldehyde. The sensor response was independent of dissolved oxygen concentration from 3 to 10 ppm and showed good stability for serum sample measurement, unlike the commonly used BSA/enzyme modified electrode. The sensor response was almost unchanged over the measurement time (>10 h) whereas the responses of a BSA and glucose oxidase modified platinum electrode and an osmium-polyvinylpyridine wired horseradish peroxidase modified electrode (Ohara et al., 1993) fell to 68% of their initial value in a serum sample containing 10mM glucose.
Kurita
Ryoji
R
NTT Advanced Technology, 3-1 Morinosato-Wakamiya, Atsugi, Kanagawa 243-0198, Japan. kurita@atsugi.ntt-at.co.jp
Tabei
Hisao
H
Iwasaki
Yuzuru
Y
Hayashi
Katsuyoshi
K
Sunagawa
Kenji
K
Niwa
Osamu
O
D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry*; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
D001672:Biocompatible Materials; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D008567:Membranes, Artificial; D014753:Vinyl Compounds; C500579:vinylferrocene; D012710:Serum Albumin, Bovine; D005949:Glucose Oxidase; D005947:Glucose; D005976:Glutaral