Viral load of human herpesvirus 8 (HHV-8) in the circulatory blood cells correlates with clinical progression in a patient with HHV-8-associated solid lymphoma with aids-associated Kaposi's sarcoma.
We encountered a case of a rapidly progressive HHV-8-associated solid lymphoma with AIDS-associated Kaposi's sarcoma (KS). HHV-8 DNA load in whole blood cells was analyzed quantitatively by real-time PCR using amplification of the HHV-8-encoded ORF26 gene. Ours is the first observation that the rapid increase in the HHV-8 viral load (from 1.9x10(4) copies/microg to 1.6x10(6) copies/microg in 40 days) in conjunction with low CD4+ cell counts was accompanied by an accelerated clinical disease progression. The results indicate that the quantity of circulating HHV-8 is measurable with real-time PCR and can provide clinically useful information.
Song
Jian
J
Department of Medical Science I, School of Health and Sport Sciences, Osaka University, Osaka, Japan.
Yoshida
Atsushi
A
Yamamoto
Yoshihiko
Y
Katano
Harutaka
H
Hagihara
Keisuke
K
Oka
Shinichi
S
Kimura
Satoshi
S
Yoshizaki
Kazuyuki
K
D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D015704:CD4 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D004279:DNA, Viral / Q000378:metabolism; D018450:Disease Progression; D019288:Herpesvirus 8, Human / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000821:virology; D008297:Male; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*; D013997:Time Factors; D019562:Viral Load
D015704:CD4 Antigens; D004279:DNA, Viral