title: Isoflavonoids in the Rutaceae family: 1. Fortunella obovata, Murraya paniculata and four Citrus species.
abstract: Several types of compounds with immunoreactivity similar to isoflavonoids were detected in water: ethanol extracts of leaves of Fortunella obovata Hort. ex Tanaka, Murraya paniculata Jack. and four Citrus species, namely C. aurantium L, C. grandis Osbeck, C. limonia Osbeck., and C. sinensis Osbeck (Rutaceae). The chromatographic mobilities of the immunoreactive substances were compared with those of authentic standards, revealing a spectrum of isoflavonoid metabolites in all plants studied. Aglycones as well as glycosides were recognized, namely daidzin, genistin, daidzein, genistein, formononetin, biochanin A, prunetin, and several incompletely characterized isoflavonoids. A subsequent HPLC-MS study verified the identities of the main immunoreactive isoflavonoids and established the identities of several others, viz. glycitein, glycitin, ononin and sissotrin, including the malonylated and acetylated isoflavonoid glucosides. The estimated content of the individual immunoreactive entities ranged from a few microg to about 2 mg/kg (dry weight). It is concluded that the isoflavonoid metabolic pathway is present throughout the Rutaceae family.
authors.affiliation: Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Prague, Czech Republic. oldrich.lapcik@vscht.cz
authors.forename: Oldrich
authors.initials: O
authors.lastname: Lapcík
authors.forename: Borivoj
authors.initials: B
authors.lastname: Klejdus
authors.forename: Michaela
authors.initials: M
authors.lastname: Davidová
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kokoska
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kubán
authors.forename: Jitka
authors.initials: J
authors.lastname: Moravcová
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D019833:Genistein / Q000737:chemistry / Q000378:metabolism; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011863:Radioimmunoassay; D027821:Rutaceae / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007529:Isoflavones; C004742:daidzein; D019833:Genistein