title: Glycosidic torsional motions in a bicelle-associated disaccharide from residual dipolar couplings.
abstract: An analysis of torsional motions about glycosidic bonds in a disaccharide is undertaken using residual dipolar coupling measurements and selective immobilization of the reducing end sugar to provide a suitable motional reference. The immobilization is accomplished by using the short chain of an alkyl glycoside to anchor the disaccharide to a bilayer medium aligned in magnetic field. Motions about the beta-(1-4) linkage of the n-butyl-4-O-beta-d-galactopyranosyl-alpha-d-mannopyranoside are shown to be substantial (+/-40 degrees ) and in good agreement with predictions of a fully solvated molecular dynamics simulation.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yi
authors.forename: André
authors.initials: A
authors.lastname: Venot
authors.forename: John
authors.initials: J
authors.lastname: Glushka
authors.forename: James H
authors.initials: JH
authors.lastname: Prestegard
mesh_terms: D002236:Carbohydrate Conformation; D004187:Disaccharides / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D008958:Models, Molecular; D013816:Thermodynamics
chemical_list: D004187:Disaccharides; D006027:Glycosides; D008358:Mannose; D005690:Galactose