title: Host range factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-Ld652Y cells derived from L. dispar.
abstract: Host range factor 1 (HRF-1) of Lymantria dispar multinucleocapsid nucleopolyhedrovirus promotes Autographa californica MNPV replication in nonpermissive Ld652Y cells derived from L. dispar. Here we demonstrate that restricted Hyphantria cunea NPV replication in Ld652Y cells was not due to apoptosis but was likely due to global protein synthesis arrest that could be restored by HRF-1. Our data also showed that HRF-1 promoted the production of progeny virions for two other baculoviruses, Bombyx mori NPV and Spodoptera exigua MNPV, whose replication in Ld652Y cells is limited to replication of viral DNA without successful production of infectious progeny virions. Thus, HRF-1 is an essential viral factor required for productive infection of NPVs in Ld652Y cells.
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Motoko
authors.initials: M
authors.lastname: Ikeda
authors.forename: Cristiano A Felipe
authors.initials: CA
authors.lastname: Alves
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Thiem
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D017209:Apoptosis; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
chemical_list: C099608:HRF-1 protein, Autographa californica nucleopolyhedrovirus; D014764:Viral Proteins