title: Nitrogen-fixing bacterium Burkholderia brasiliensis produces a novel yersiniose A-containing O-polysaccharide.
abstract: Burkholderia brasiliensis, a Gram-negative diazotrophic endophytic bacterium, was first isolated from roots, stems, and leaves of rice plant in Brazil. The polysaccharide moiety was released by ammonolysis from the B. brasiliensis lipopolysaccharide (LPS), allowing the unambiguous characterization of a 3,6-dideoxy-4-C-(1-hydroxyethyl)-D-xylo-hexose (yersiniose A), an uncommon feature for Burkholderia LPS. The complete structure of the yersiniose A-containing O-antigen was identified by sugar and methylation analyses and NMR spectroscopy. Our results show that the repeating oligosaccharide motif of LPS O-chain consists of a branched tetrasaccharide with the following structure:-->2-alpha-d-Rhap-(1-->3)-[alpha-YerAp-(1-->2)]-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Universidade Federal do Rio de Janeiro, 21944-970, Brazil.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Mattos
authors.forename: Adriane R
authors.initials: AR
authors.lastname: Todeschini
authors.forename: Norton
authors.initials: N
authors.lastname: Heise
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
authors.forename: Jose O
authors.initials: JO
authors.lastname: Previato
authors.forename: Lucia
authors.initials: L
authors.lastname: Mendonça-Previato
mesh_terms: D019117:Burkholderia / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D006601:Hexoses / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D019081:O Antigens / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D006601:Hexoses; D019081:O Antigens; C041266:yersiniose; D009584:Nitrogen