title: Initial assessment of a tissue engineered stomach derived from syngeneic donors in a rat model.
abstract: The objective of this study is to assess the feasibility of creating a tissue engineered stomach using isolated stomach epithelium organoid unit from syngeneic adult donors and a biodegradable polymer scaffold in a rat model. Despite recent advances in reconstruction techniques, total gastrectomy is still accompanied by various complications. As an alternative treatment, a tissue engineered stomach that replaces the mechanical and metabolic functions of a normal stomach is proposed. Stomach epithelium organoid units were isolated from syngeneic adult rats and seeded onto biodegradable polymers. These constructs were implanted into the omenta of recipient adult rats. All constructs were harvested for histologic and immunohistochemical examination at designated time points. Cyst-like structures were formed that showed the development of vascularized tissue with a neomucosa. Immunohistochemical staining for alpha-actin smooth muscle, gastric mucin, and proton pump indicated the presence of a smooth muscle layer and gastric epithelium, as well as the existence of parietal cells of the stomach mucosa, respectively. Epithelium derived stomach organoid units seeded on biodegradable polymers were transplanted in donor rats and have been shown to vascularize, survive, and regenerate into complex tissue resembling a native stomach. These initial results are encouraging, and studies are currently underway to further assess this approach.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, USA.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Maemura
authors.forename: Michael
authors.initials: M
authors.lastname: Shin
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishii
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Vacanti
mesh_terms: D020341:Absorbable Implants; D000199:Actins / Q000378:metabolism; D000818:Animals; D005753:Gastric Mucosa / Q000033:anatomy & histology / Q000502:physiology*; D007150:Immunohistochemistry; D019344:Lactic Acid; D009130:Muscle, Smooth / Q000378:metabolism; D009940:Organoids / Q000033:anatomy & histology / Q000502:physiology; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D017494:Proton Pumps / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013270:Stomach / Q000033:anatomy & histology / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000199:Actins; D011091:Polyesters; D011108:Polymers; D017494:Proton Pumps; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)