DARPP-32-like immunoreactivity in AII amacrine cells of rat retina.
Previous studies demonstrated that the dopamine- and adenosine 3',5'-monophosphate-regulated phosphatase inhibitor known as "DARPP-32" is present in rat, cat, monkey, and human retinas. We have followed up these studies by asking what specific cell subtypes contain DARPP-32. Using a polyclonal antibody directed against a peptide sequence of human DARPP-32, we immunostained adult rat retinas that were either transretinally sectioned or flat mounted and found DARPP-32-like immunoreactivity in some cells of the amacrine cell layer across the entire retinal surface. We report here, based on the shape and spatial distribution of these cells, their staining by an anti-parvalbumin antibody, and their juxtaposition with processes containing tyrosine hydroxylase, that DARPP-32-like immunoreactivity is present in AII amacrine cells of rat retina. These results suggest that the response of AII amacrine cells to dopamine is not mediated as simply as previously supposed.
Partida
Gloria J
GJ
Section of Neurobiology, Physiology, and Behavior, University of California, Davis, Davis, California 95616-8519, USA.
Lee
Sherwin C
SC
Haft-Candell
Leah
L
Nichols
Grant S
GS
Ishida
Andrew T
AT
D025042:Amacrine Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005260:Female; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000378:metabolism*
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D010750:Phosphoproteins