[Study on relationship between the polymorphism of angiotensin converting enzyme gene and blood stasis syndrome in patients with coronary heart disease].
OBJECTIVE
To explore the relationship between the insertion/deletion (I/D) polymorphism of angiotensin converting enzyme (ACE), and blood stasis syndrome (BSS) in patients with coronary heart disease (CHD).


METHODS
The ACE gene type in 48 patients of CHD of BSS type, 52 CHD patients of non-BSS type and 54 healthy subjects (control) was determined by PCR assay, also levels of endothelin (ET), angiotensin II (Ag II), and nitric oxide (NO) were determined.


RESULTS
Occurrence of DD genotype and allele genotype of ACE gene was higher in patients of BSS than that in patients of non-BSS and control (P < 0.01). ET/NO level was higher in patients of BSS than that in control (P < 0.01). ET and Ag II levels in patients of BSS were significantly higher than those in patients of non-BSS (P < 0.05) and control (P < 0.01). Levels of ET/NO and Ag II in subjects with DD genotype in various groups were higher than those in subjects with Ag II or ID genotype, the highest level occurred in patients of BSS with DD genotype, when compared with the other two groups, the difference in Ag II was significant (P < 0.05 and P < 0.01), when compared with control, the difference in ET/NO was significant (P < 0.01).


CONCLUSIONS
DD genotype of ACE gene may be the susceptible gene of CHD in patients of BSS type.
Mao
Yi-lin
YL
Discipline of TCM Diagnostics, Hunan TCM College, Changsha (410005).
Yuan
Zhao-kai
ZK
Huang
Xian-ping
XP
Lu
Fangguo
F
Tan
Guangbo
G
Hu
Zhixi
Z
D000368:Aged; D000483:Alleles; D000787:Angina Pectoris / Q000235:genetics; D000804:Angiotensin II / Q000097:blood; D003324:Coronary Artery Disease / Q000235:genetics*; D003937:Diagnosis, Differential; D016232:Endothelins / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic
D016232:Endothelins; D000804:Angiotensin II; D009569:Nitric Oxide; D007703:Peptidyl-Dipeptidase A