[Bacteriophage of Bacillus polymyxa BC153-29].
A virulent bacteriophage has been isolated from culture liquid of the sowing and production fermenter in the process of biosynthesis of polymyxin B; the bacteriophage lyses a sensitive producer's culture of antibiotic Bacillus polymyxa BC153-29. The bacteriophage has been purified by ultracentrifugation in the density gradient of cesium chloride, and its morphology has been investigated by the election microscopy. The hexagonal phage head is 72 +/- 1.8 nm in diameter, with the length and width of the elastic noncontractile appendix--300 +/- 2.3 and 14.2 +/- 0.5 nm, respectively. The phage was related to Siphoviridae (B1) family. The phage inactivation dynamics has been studied using specific antiserum, the phage neutralization velocity constant (595 min(-1)) and phage adsorption velocity constant on sensitive cells (9.39 x 10(-8) ml/min) have been determined. Exogenic origin of the isolated virulent bacteriophage of Bacillus polymyxa BC153-29 has been supposed.
Matseliukh
D Ia
DIa
Burova
L M
LM
D001407:Bacillus / Q000648:ultrastructure / Q000821:virology*; D017103:Bacillus Phages / Q000235:genetics / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron