title: Role of apoptosis in functional luteolysis in the pregnant rabbit corpus luteum: evidence of a role for placental-derived factors in promoting luteal cell survival.
abstract: Corpora lutea (CL) were isolated from one rabbit ovary on days 4, 8, 16 (peak luteal function), 28 (functional regression) and 30 of pregnancy and processed for biochemical analysis of DNA integrity. Analysis of DNA integrity revealed the presence of oligonucleosomal fragments in day 28 and day 30 CL but not in day 16 CL. The extent of low molecular weight (<15 kb) DNA labeling was 6.6 +/- 0.84 fold higher in day 30 as compared to day 16 CL (mean +/- SEM; n = 4, P < 0.01). In a second series of experiments, healthy CL collected from day 16 pregnant rabbits were incubated for 2 h in the absence or presence of 250 microg/ml of placental extract (PE) obtained from day 16 and/or day 30 placentas. Analysis of DNA integrity revealed that extensive apoptosis occurred in CL incubated in medium alone and in medium containing day 30 PE. In contrast, day 16 PE significantly suppressed apoptosis vs control (70 +/- 4%). In a third series of experiments, expression of mRNA for bcl-x and bax was measured by Northern analysis of CL treated without and with day 16 PE using cRNA probes for bcl-x and bax developed in our laboratory by RT-PCR. Treatment with PE significantly reduced bax mRNA levels but did not change bcl-x mRNA levels. These studies provide evidence that functional luteolysis in the pregnant rabbit CL is correlated with the occurrence of apoptosis. The data suggest that a factor(s) derived from the placenta may be responsible for the prolongation of CL life span during pregnancy by its ability to alter the bax:bcl -x rheostat and suppress apoptosis.
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. dharma@anhb.uwa.edu.au
authors.forename: A M
authors.initials: AM
authors.lastname: Dharmarajan
authors.forename: S B
authors.initials: SB
authors.lastname: Goodman
authors.forename: N
authors.initials: N
authors.lastname: Atiya
authors.forename: S P
authors.initials: SP
authors.lastname: Parkinson
authors.forename: R R
authors.initials: RR
authors.lastname: Lareu
authors.forename: K I
authors.initials: KI
authors.lastname: Tilly
authors.forename: J L
authors.initials: JL
authors.lastname: Tilly
mesh_terms: D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D003338:Corpus Luteum / Q000502:physiology*; D005260:Female; D003341:Luteolysis; D009924:Organ Culture Techniques; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy / Q000502:physiology*; D011374:Progesterone / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D011863:Radioimmunoassay; D013997:Time Factors; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D011374:Progesterone