Polymorphisms in the tumor necrosis factor-alpha gene at position -308 and the inducible 70 kd heat shock protein gene at position +1267 in multifetal pregnancies and preterm premature rupture of fetal membranes.
OBJECTIVE
The purpose of this study was to determine the relationship between preterm premature rupture of membranes, tumor necrosis factor-alpha, and heat shock protein-70 gene polymorphisms in multifetal gestations.


METHODS
Buccal swabs from 101 mother-neonate pairs of multifetal pregnancies were tested for single nucleotide polymorphisms at position -308 of the tumor necrosis factor-alpha gene and +1267 of the heat shock protein-70 gene. Pregnancy outcome data were obtained subsequently.


RESULTS
Tumor necrosis factor-alpha allele 2 carriage by the first-born occurred in 10 of 27 pregnancies (37.0%) that resulted in preterm premature rupture of membranes compared with 6 of 67 pregnancies (9.0%) without preterm premature rupture of membranes ( P = .002). The allele frequency of tumor necrosis factor-alpha allele 2 and heat shock protein-70 allele 2 in the first born was higher in pregnancies that were complicated by preterm premature rupture of membranes (18.5% vs 4.5%; P = .003; and 57.7% vs 41.3%; P = .04, respectively). There was no relationship between tumor necrosis factor-alpha allele 2 or heat shock protein-70 allele 2 carriage by the second fetus or mother and preterm premature rupture of membranes.


CONCLUSIONS
Tumor necrosis factor-alpha allele 2 and/or heat shock protein-70 allele 2 carriage by the first-born fetus is associated with preterm premature rupture of membranes in multifetal pregnancies.
Kalish
Robin B
RB
Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, New York Weill Medical College of Cornell University, 525 East 68th Street, New York, NY 10021, USA.
Vardhana
Santosh
S
Gupta
Meruka
M
Perni
Sriram C
SC
Chasen
Stephen T
ST
Witkin
Steven S
SS
D000483:Alleles; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011247:Pregnancy; D011272:Pregnancy, Multiple / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D018840:HSP70 Heat-Shock Proteins; D014409:Tumor Necrosis Factor-alpha