title: Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo.
abstract: Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Müllerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Müllerian duct in the developing chicken embryo. Morphological changes in the Müllerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Müllerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Müllerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Müllerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Müllerian duct in the female embryos of the chicken.
authors.affiliation: Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan.
authors.forename: Yonju
authors.initials: Y
authors.lastname: Ha
authors.forename: Akira
authors.initials: A
authors.lastname: Tsukada
authors.forename: Noboru
authors.initials: N
authors.lastname: Saito
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000254:growth & development*; D002645:Chickens; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D009095:Mullerian Ducts / Q000196:embryology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2