title: The combination of PRRS virus and bacterial endotoxin as a model for multifactorial respiratory disease in pigs.
abstract: This paper reviews in vivo studies on the interaction between porcine reproductive and respiratory syndrome virus (PRRSV) and LPS performed in the authors' laboratory. The main aim was to develop a reproducible model to study the pathogenesis of PRRSV-induced multifactorial respiratory disease. The central hypothesis was that respiratory disease results from an overproduction of proinflammatory cytokines in the lungs. In a first series of studies, PRRSV was shown to be a poor inducer of TNF-alpha and IFN-alpha in the lungs, whereas IL-1 and the anti-inflammatory cytokine IL-10 were produced consistently during infection. We then set up a dual inoculation model in which pigs were inoculated intratracheally with PRRSV and 3-14 days later with LPS. PRRSV-infected pigs developed acute respiratory signs for 12-24h upon intratracheal LPS inoculation, in contrast to pigs inoculated with PRRSV or LPS only. Moreover, peak TNF-alpha, IL-1 and IL-6 titers were 10-100 times higher in PRRSV-LPS inoculated pigs than in the singly inoculated pigs and the cytokine overproduction was associated with disease. To further prove the role of proinflammatory cytokines, we studied the effect of pentoxifylline, a known inhibitor of TNF-alpha and IL-1, on PRRSV-LPS induced cytokine production and disease. The clinical effects of two non-steroidal anti-inflammatory drugs (NSAIDs), meloxicam and flunixin meglumine, were also examined. Pentoxifylline, but not the NSAIDs, significantly reduced fever and respiratory signs from 2 to 6h after LPS. The levels of TNF-alpha and IL-1 in the lungs of pentoxifylline-treated pigs were moderately reduced, but were still 26 and 3.5-fold higher than in pigs inoculated with PRRSV or LPS only. This indicates that pathways other than inhibition of cytokine production contributed to the clinical improvement. Finally, we studied a mechanism by which PRRSV may sensitize the lungs for LPS. We hypothesized that PRRSV would increase the amount of LPS receptor complex in the lungs leading to LPS sensitisation. Both CD14 and LPS-binding protein, two components of this complex, increased significantly during infection and the amount of CD14 in particular was correlated with LPS sensitisation. The increase of CD14 was mainly due to infiltration of strongly CD14-positive monocytes in the lungs. The PRRSV-LPS combination proved to be a simple and reproducible experimental model for multifactorial respiratory disease in pigs. To what extent the interaction between PRRSV and LPS contributes to the development of complex respiratory disease is still a matter of debate.
authors.affiliation: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Steven
authors.initials: S
authors.lastname: Van Gucht
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Labarque
authors.forename: Kristien
authors.initials: K
authors.lastname: Van Reeth
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008954:Models, Biological; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000382:microbiology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D012141:Respiratory Tract Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology
chemical_list: D001427:Bacterial Toxins; D016207:Cytokines; D008070:Lipopolysaccharides; D010726:Phosphodiesterase Inhibitors; D011448:Prostaglandin Antagonists