title: Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments.
abstract: The organization of secretory traffic remains unclear, mainly because of the complex structure and dynamics of the secretory pathway. We have thus studied a simplified system, a single synchronized traffic wave crossing an individual Golgi stack, using electron tomography. Endoplasmic-reticulum-to-Golgi carriers join the stack by fusing with cis cisternae and induce the formation of intercisternal tubules, through which they redistribute their contents throughout the stack. These tubules seem to be pervious to Golgi enzymes, whereas Golgi vesicles are depleted of both enzymes and cargo. Cargo then traverses the stack without leaving the cisternal lumen. When cargo exits the stack, intercisternal connections disappear. These findings provide a new view of secretory traffic that includes dynamic intercompartment continuities as key players.
authors.affiliation: Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy.
authors.forename: Alvar
authors.initials: A
authors.lastname: Trucco
authors.forename: Roman S
authors.initials: RS
authors.lastname: Polishchuk
authors.forename: Oliviano
authors.initials: O
authors.lastname: Martella
authors.forename: Alessio
authors.initials: A
authors.lastname: Di Pentima
authors.forename: Aurora
authors.initials: A
authors.lastname: Fusella
authors.forename: Daniele
authors.initials: D
authors.lastname: Di Giandomenico
authors.forename: Enrica
authors.initials: E
authors.lastname: San Pietro
authors.forename: Galina V
authors.initials: GV
authors.lastname: Beznoussenko
authors.forename: Elena V
authors.initials: EV
authors.lastname: Polishchuk
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Baldassarre
authors.forename: Roberto
authors.initials: R
authors.lastname: Buccione
authors.forename: Willie J C
authors.initials: WJ
authors.lastname: Geerts
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Koster
authors.forename: Koert N J
authors.initials: KN
authors.lastname: Burger
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Mironov
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
mesh_terms: D001692:Biological Transport; D002451:Cell Compartmentation; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure*; D008854:Microscopy, Electron