Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long-lasting immunity.
In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS-CoV) epitope peptides capable of inducing long-lasting immunity, we first screened immunoglobulin-G (IgG) antibodies reactive to 197 different overlapping 15-mers from the SARS-CoV proteins in the sera of three infected patients. Forty-two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6-month post-infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post-infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long-lasting immunity, may provide a better understanding of the immunogenicity of SARS-CoV.
Shichijo
S
S
Department of Immunology, Kurume University School of Medicine, Asahi-machi, Kurume, Fukuoka, Japan.
Keicho
N
N
Long
H T
HT
Quy
T
T
Phi
N C
NC
Ha
L D
LD
Ban
V V
VV
Itoyama
S
S
Hu
C-J
CJ
Komatsu
N
N
Kirikae
T
T
Kirikae
F
F
Shirasawa
S
S
Kaji
M
M
Fukuda
T
T
Sata
M
M
Kuratsuji
T
T
Itoh
K
K
Sasazuki
T
T
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007107:Immune System / Q000276:immunology*; D007109:Immunity / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D010455:Peptides / Q000276:immunology*; D012212:Rheology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D044967:Serum / Q000276:immunology
D007074:Immunoglobulin G; D010455:Peptides