Separation of flavonoids from the seeds of Vernonia anthelmintica Willd by high-speed counter-current chromatography.
Several flavonoids including 2',3,4,4'-tetrahydroxychalcone, 5,6,7,4'-tetrahydroxyflavone and butin, were separated from the seeds of Vernonia anthelmintica Willd by high-speed counter-current chromatography using a two-step operation. Two different types of solvent systems were used: chloroform-dichloromethane-methanol-water (2:2:3:2, v/v) and 1,2 dichloroethane-methanol-acetonitrile-water (4:1.1:0.25:2, v/v). From 1 kg of seeds of Vernonia anthelmintica Willd the method yielded about 45 mg of 2',3,4,4'-tetrahydroxychalcone, 40mg of 5,6,7,4'-tetrahydroxyflavone, and 55 mg of butin. Each isolated component showed 95-97% purity as determined by high-performance liquid chromatography analysis. These purified compounds were characterized by MS and NMR.
Tian
Guilian
G
Beijing Institute of New Technology Application, Beijing 100035, China.
Zhang
Ubin
U
Zhang
Tianyou
T
Yang
Fuquan
F
Ito
Yoichiro
Y
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012639:Seeds / Q000737:chemistry; D035741:Vernonia / Q000737:chemistry*
D005419:Flavonoids; D007202:Indicators and Reagents