title: EPR and affinity studies of mannose-TEMPO functionalized PAMAM dendrimers.
abstract: Mannose-TEMPO functionalized G4-PAMAM dendrimers with increasing mannose loadings have been synthesized and characterized by MALDI-TOF MS and EPR spectroscopy. Analysis of linebroadening effects in the EPR spectra of these dendrimers allowed us to determine the relative presentation of mannose and TEMPO on the dendrimer surface. Hemagglutination assays and affinity chromatography/EPR experiments to assess the activity of the mannose-TEMPO dendrimers with Concanavalin A are presented.
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
authors.forename: Lynn E
authors.initials: LE
authors.lastname: Samuelson
authors.forename: Karl B
authors.initials: KB
authors.lastname: Sebby
authors.forename: Eric D
authors.initials: ED
authors.lastname: Walter
authors.forename: David J
authors.initials: DJ
authors.lastname: Singel
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Cloninger
mesh_terms: D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000737:chemistry*; D050091:Dendrimers; D004578:Electron Spin Resonance Spectroscopy; D006386:Hemagglutination Tests; D008358:Mannose / Q000737:chemistry*; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D013113:Spin Labels; D003208:Concanavalin A; D008358:Mannose; C003959:TEMPO