title: Genotoxicity evaluation of Isaria sinclairii (ISE) extract.
abstract: The mutagenic potential Isaria sinclairii, a traditional Chinese medicine composed of the fruiting bodies of I. sinclairii and its parasitic host larva, was evaluated using short-term genotoxicity tests, namely, the Ames test, chromosome aberration (CA), and micronuclei (MN) tests. In a Salmonella typhimurium assay, I. sinclairii extract (ISE) did not produce any mutagenic response in the absence or presence of 59 mix with TA98, TA100, TA1535, and TA1537. In the chromosome aberration (CA) test, ISE induced no significant effect on Chinese hamster ovary (CHO) cells compared with control. In the MN test, no significant change in the occurrence of micronucleated polychromatic erythrocytes was observed in male ICR mice intraperitoneally administered ISE at doses of 15, 150, or 1500 mg/kg. These results indicate that ISE has no mutagenic potential in these in vitro and in vivo systems.
authors.affiliation: Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Kang Sun
authors.initials: KS
authors.lastname: Ryu
authors.forename: Sang Duck
authors.initials: SD
authors.lastname: Jee
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: In Sun
authors.initials: IS
authors.lastname: Kim
authors.forename: Se C
authors.initials: SC
authors.lastname: Kang
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Koo
authors.forename: Yo An
authors.initials: YA
authors.lastname: Park
authors.forename: Sul Min
authors.initials: SM
authors.lastname: Choi
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Yoo
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kwack
authors.forename: Sun Dong
authors.initials: SD
authors.lastname: Yoo
authors.forename: Byung Mu
authors.initials: BM
authors.lastname: Lee
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D016466:CHO Cells; D002869:Chromosome Aberrations / Q000139:chemically induced*; D006224:Cricetinae; D003412:Cricetulus; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D004912:Erythrocytes; D015162:Micronucleus Tests; D009152:Mutagenicity Tests
chemical_list: D004365:Drugs, Chinese Herbal