Novel member of the PAR domain bZIP gene family isolated from Pacific oyster, Crassostrea gigas.
A cDNA encoding the first mollusk member of the PAR subfamily of bZIP transcription factors has been characterized in the Pacific oyster, Crassostrea gigas. The sequence of the cDNA predicts a protein of 260 amino acids that has 41-50% identity with the PAR domain, 72-79% identity with the basic DNA-binding domain and 34-56% identity with the leucine zipper domain of other members of the PAR subfamily. Polymerase chain reaction with reverse transcription indicates that this gene is expressed during developmental stages from an unfertilized egg to a juvenile. It was demonstrated by northern hybridization that the gene is also expressed on several adult tissues. The identification of a novel member of the PAR subfamily bZIP genes in mollusks may help to identify common functions that have been conserved through evolution and to elucidate evolutionary relationships within this subfamily of proteins.
Hamano
Kaoru
K
Physiology and Molecular Biology Division, National Research Institute of Fisheries Science, Yokohama 236-8648, Japan. hamano@affrc.go.jp
Awaji
Masahiko
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D015152:Blotting, Northern; D016000:Cluster Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051790:G-Box Binding Factors; D020869:Gene Expression Profiling; D007564:Japan; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010125:Ostreidae / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D014157:Transcription Factors