title: Genetic analysis of the M RNA segment of Crimean-Congo hemorrhagic fever virus strains involved in the recent outbreaks in Russia.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is a severe zoonosis with a high fatality rate. In Russia, local CCHF outbreaks have occurred in the Stavropol Territory, and the Volgograd and Astrakhan Regions during 2000 and 2001. Seven strains of CCHF virus (CCHFV) were isolated from infected patients and collected ticks. Two fragments of the CCHF virus M genome segment were PCR amplified and their nucleotide sequences were determined. All these virus strains appear to be closely related (up to 5.8% nucleotide sequence differences) and form a distinct clade on the CCHFV phylogenetic tree. Within this clade, CCHFV strains from Stavropol and Astrakhan cluster together, whereas those from Volgograd form a separate subgroup.
authors.affiliation: Charité - Universitätsmedizin Berlin, Institut für Infektionsmedizin, Berlin, Germany.
authors.forename: J H
authors.initials: JH
authors.lastname: Kuhn
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: S P
authors.initials: SP
authors.lastname: Morzunov
authors.forename: I D
authors.initials: ID
authors.lastname: Petrova
authors.forename: O I
authors.initials: OI
authors.lastname: Vyshemirskii
authors.forename: D K
authors.initials: DK
authors.lastname: Lvov
authors.forename: G I
authors.initials: GI
authors.lastname: Tyunnikov
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
mesh_terms: D000595:Amino Acid Sequence; D004196:Disease Outbreaks; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics*; D006479:Hemorrhagic Fever, Crimean / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012426:Russia
chemical_list: D012367:RNA, Viral