title: A mass spectrometric investigation of non-covalent interactions between ruthenium complexes and DNA.
abstract: Electrospray ionisation mass spectrometry was used to investigate reactions between six ruthenium compounds and three different non self-complementary duplex oligonucleotides containing 16 base pairs. Each of the compounds studied formed non-covalent complexes containing between one and five ruthenium molecules bound to DNA. Competition experiments involving duplex 16mers and pairs of ruthenium compounds were used to determine the order of relative binding affinities of the metal compounds. Other competition experiments involving ruthenium compounds, and the organic DNA binding agents daunomycin and distamycin, provided information about the sites and modes of DNA binding of the ruthenium compounds.
authors.affiliation: Department of Chemistry, University of Wollongong, Northfields Avenue, New South Wales, Australia.
authors.forename: Thitima
authors.initials: T
authors.lastname: Urathamakul
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Beck
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Sheil
authors.forename: Janice R
authors.initials: JR
authors.lastname: Aldrich-Wright
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Ralph
mesh_terms: D004247:DNA / Q000737:chemistry*; D003609:Dactinomycin / Q000737:chemistry; D003630:Daunorubicin / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009942:Organometallic Compounds; D003609:Dactinomycin; D012428:Ruthenium; D004247:DNA; D003630:Daunorubicin