title: Electrophoretic variants of intracellular catalase of different Candida species.
abstract: Intracellular and extracellular catalases of different species of Candida were investigated using different culture media. All the Candida strains produced intracellular catalase, whose enzymatic activity was detected by non-denaturating polyacrylamide gradient (4-30%) gel electrophoresis. The cell extracts presented a major 230 kDa catalase band and in some strains variants of catalase with different molecular weights were detected. Candida catalase activity was not affected by heating at 50 degrees C and incubation with beta-mercaptoethanol, but treatment with sodium dodecyl sulphate inhibited or reduced enzymatic activity. Extracellular enzyme activity was not detected in any of the culture filtrate extracts tested.
authors.affiliation: Institute of Biomedical Science, São Paulo University, USP, SP, Brazil.
authors.forename: N R S
authors.initials: NR
authors.lastname: Miyasak
authors.forename: C S
authors.initials: CS
authors.lastname: Unterkircher
authors.forename: P O
authors.initials: PO
authors.lastname: Carvalho
authors.forename: M T
authors.initials: MT
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D002175:Candida / Q000145:classification* / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D002180:Candidiasis, Oral / Q000382:microbiology / Q000503:physiopathology; D002374:Catalase / Q000378:metabolism*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004795:Enzyme Stability; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D014774:Virulence
chemical_list: D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D002374:Catalase