title: Variability of markers of inflammation and infection in induced sputum in children with cystic fibrosis.
abstract: To determine reproducibility of inflammatory marker concentrations in induced sputum from subjects with cystic fibrosis (CF), 15 nonexpectorating children, 6 to 13 years of age with mild CF lung disease, underwent 3 weekly sputum inductions with 3% saline. Neutrophil elastase concentration and bacterial cultures were reproducible. This study provides useful information for investigators designing trials of anti-inflammatory therapies in CF involving sputum induction.
authors.affiliation: Department of Medicine and Clinical Research Program, Children's Hospital Boston, Massachusetts 02115, USA. claudia.ordonez@childrens.harvard.edu
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Ordoñez
authors.forename: Alex I
authors.initials: AI
authors.lastname: Kartashov
authors.forename: Mary Ellen B
authors.initials: ME
authors.lastname: Wohl
mesh_terms: D000280:Administration, Inhalation; D000293:Adolescent; D002648:Child; D003550:Cystic Fibrosis / Q000201:enzymology* / Q000382:microbiology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D019272:Leukocyte Elastase / Q000378:metabolism; D009330:Nebulizers and Vaporizers; D009195:Peroxidase / Q000378:metabolism; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000008:administration & dosage; D013183:Sputum / Q000201:enzymology* / Q000382:microbiology*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D012965:Sodium Chloride; D009195:Peroxidase; D019272:Leukocyte Elastase