On the mechanism of cell lysis by deformation.
In this study, we identify the extent of deformation that causes cell lysis using a simple technique where a drop of cell suspension is compressed by two flat plates. The viability of human prostatic adenocarcinoma PC-3 cells in solutions of various concentrations of NaCl is determined as a function of the gap size between the plates. The viability declines with decreasing gap size in the following order: 700 mM >150 mM >75 mM NaCl. This is considered to be due to the difference in cell size, which is caused by the osmotic volume change before deformation; cell diameter becomes smaller in a solution of higher NaCl concentration, which appears to increase the survival ratio in a given gap size. The deformation-induced decrease in cell viability is correlated with the cell surface strain, which is dependent on the increase in surface area, irrespective of NaCl concentration. In addition, the treatment of cells with cytochalasin D results in the disappearance of cortical actin filaments and a marked drop in the viability, indicating that cell lysis is closely related to the deformation of the cytoskeleton.
Takamatsu
Hiroshi
H
Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasugakoen, Kasuga, Fukuoka 816-8580, Japan. takamatu@cm.kyushu-u.ac.jp
Takeya
Ryu
R
Naito
Seiji
S
Sumimoto
Hideki
H
D008841:Actin Cytoskeleton / Q000187:drug effects; D001696:Biomechanical Phenomena; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000648:ultrastructure; D048430:Cell Shape; D048429:Cell Size; D002470:Cell Survival / Q000187:drug effects; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D009994:Osmolar Concentration; D009995:Osmosis; D011312:Pressure; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D013314:Stress, Mechanical
D015638:Cytochalasin D; D012965:Sodium Chloride