Shock waves increase T-cell proliferation or IL-2 expression by activating p38 MAP kinase.
Shock waves were elicited by transient pressure disturbances, which could be used to treat musculoskeletal disorders. In present studies, we investigated whether the low-density shock waves (LDSWs), which are able to damage plasma membrane without impairing the vimentin or other organelles, might augment T-cell proliferation as well as IL-2 expression, and if mitogen activated protein kinase p38 (p38 MAPK) might be an underlying mechanism through which the LDSWs enhanced T-cell function. We found that the LDSWs increased activation of p38 MAPK in Jurkat T cells. The LDSWs alone didn't result in the T-cell proliferation and IL-2 expression. However, in combination with other stimuli, LDSWs could augment the T-cell proliferation and IL-2 expression. Inhibition of p38 MAPK using SB203580 reduced the stimulatory effects of the LDSWs, which indicated that the LDSWs enhanced IL-2 expression through a mechanism that involved p38 MAPK activation. We concluded that the p38 MAPK activation played a key role in the regulation of T cells function by the LDSWs.
Yu
Tie-Cheng
TC
Department of Orthopedics, The First Teaching Hospital of Jilin University, Changchun 130021, China. tiechengyu2003@yahoo.com.cn
Liu
Yi
Y
Tan
Yan
Y
Jiang
Yanfang
Y
Zheng
Xueqing
X
Xu
Xinxiang
X
D017252:CD3 Complex / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D008954:Models, Biological; D011725:Pyridines / Q000494:pharmacology; D011840:Radiation, Nonionizing; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D017252:CD3 Complex; D016827:CD8 Antigens; D004791:Enzyme Inhibitors; D007093:Imidazoles; D007376:Interleukin-2; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580