Co-expression of interleukin-2 to increase the efficacy of DNA vaccine-mediated protection in coxsackievirus B3-infected mice.
DNA immunizations with the major structural protein VP1 of coxsackievirus B3 (CVB3) have been previously found to protect mice from a lethal challenge with CVB3. The function of this vaccination procedure is mainly based on accelerated antibody induction with an early cytokine expression and increased virus-specific cytotoxic activity of spleen cells causing decreased myocyte destruction and reduced viral replication. Here, we report that the co-expression of the immune-stimulatory interleukin-2 (IL-2) can increase the efficacy of the inoculated DNA vaccine depending on the route of administration and the mouse strain used.
Henke
Andreas
A
Institute of Virology and Antiviral Therapy, Medical Center, Friedrich Schiller University, D-07740 Jena, Germany. i6hean@rz.uni-jena.de
Chiang
Chi-Shiun
CS
Zell
Roland
R
Stelzner
Axel
A
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D029822:Enterovirus B, Human / Q000235:genetics / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007376:Interleukin-2 / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D007376:Interleukin-2; D019444:Vaccines, DNA