title: Crystallization and preliminary X-ray crystallographic studies of human 2',3'-cyclic nucleotide 3'-phosphodiesterase.
abstract: The catalytic fragment of human 2',3'-cyclic nucleotide 3'-phosphodiesterase (hCNP-CF) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 300 as the precipitating agent. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.39, b = 55.35, c = 78.76 A. There is one molecule per asymmetric unit. The crystals diffract to at least 1.8 A resolution using synchrotron radiation and are suitable for X-ray structure analysis at high resolution.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ichimiya
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kurihara
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571337:CNP protein, human