title: [The expression of PPTA and c-fos mRNA in dog caudal spinal trigeminal nucleus induced by traumatic occlusion].
abstract: OBJECTIVE
  PPTA and c-fos mRNA expression were detected in dog caudalis subnucleus of trigeminal spinal tract nucleus (VC) induced by trauma occlusion in order to investigate orofacial pain mechanism.
  
  
  METHODS
  The occlusal surface of the first and second maxillary right molars in 15 dogs were unilaterally raised 1.5 mm with casting Ni-Cr inlay which were fixed in Class I hole. On days 3, 7, 14, 30 and 60 after teeth operation, the VC of right and left sides were removed. PPTA and c-fos mRNAs were detected in experimental and control groups with reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  (1) The basal levels of PPTA and c-fos mRNAs were extremely low and poorly detectable in VC in control animals. (2) The expression of PPTA mRNA in VC of traumatic side was up regulated from 3 days after inlay was fixed in molar and reached peak level during 14 to 30 days and then down-regulated gradually and no significant difference was noted between 60 days group and control group. (3) c-fos mRNA expression was more intense during 3 to 7 days compared with the control group but undetectable in the other experimental period. (4) Both PPTA and c-fos mRNAs expression in VC of trauma occlusal side were more intense than that in the contralateral side.
  
  
  CONCLUSIONS
  The present results show that both PPTA and c-fos mRNA expression are elevated in dog's VC induced by traumatic occlusion. The primary afferent terminal of orofacial area is sensitized, which suggest one kind of mechanism of orofacial pain in the condition of traumatic occlusion.
authors.affiliation: Department of Stomatology, General Hospital of PLA, Beijing 100853, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Xin-mu
authors.initials: XM
authors.lastname: Wang
authors.forename: Da-qing
authors.initials: DQ
authors.lastname: Liu
authors.forename: Sheng-xi
authors.initials: SX
authors.lastname: Wu
mesh_terms: D000818:Animals; D003769:Dental Occlusion, Traumatic / Q000503:physiopathology*; D004285:Dogs; D005156:Facial Neuralgia / Q000209:etiology; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D015320:Tachykinins / Q000096:biosynthesis* / Q000235:genetics; D014279:Trigeminal Nucleus, Spinal / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin