title: Red clover extracts stimulate differentiation of human osteoblastic osteosarcoma HOS58 cells.
abstract: Different extracts from red clover (Trifolium pratense L.) were tested for their ability to stimulate the activity of osteoblastic osteosarcoma cells (HOS58). As a key marker of osteoblasticity we chose the activity of alkaline phosphatase (ALP). Whereas butanol and methanol extracts had no influence on either ALP or cellular protein production, enzyme activity was increased significantly on incubation with chloroform extracts. All extracts were analysed for isoflavone content. The data clearly suggest a role for red clover isoflavonoids in the stimulation of osteoblastic cell activity.
authors.affiliation: Institute of Pharmacy, University of Greifswald, Greifswald, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wende
authors.forename: L
authors.initials: L
authors.lastname: Krenn
authors.forename: I
authors.initials: I
authors.lastname: Unterrieder
authors.forename: U
authors.initials: U
authors.lastname: Lindequist
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D012516:Osteosarcoma / Q000201:enzymology; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D029921:Trifolium
chemical_list: D004968:Estrogens, Non-Steroidal; D010936:Plant Extracts; D000469:Alkaline Phosphatase