Plasmodium falciparum: production of human antibodies specific for the MSP-3 protein in the Hu-SPL-SCID mouse.
Human immunity against Plasmodium falciparum malaria is mediated by IgG antibodies. One of the major targets of protective antibodies is the MSP-3 protein. Anti-MSP-3 human monoclonal antibodies could therefore be valuable for passive immunotherapy, particularly of drug resistant malaria. Human monoclonal antibodies were previously produced in the Hu-SPL-SCID model reconstituted with human splenocytes, immunized by highly immunogenic neo-antigen or a recall antigen. We report here that this model can also be successfully employed to induce human antibody-secreting cells specific of low immunogenicity neo-antigens, such as MSP-3. These cells represent a new and valuable source of human monoclonal anti-malaria antibodies.
Bouharoun-Tayoun
Hasnaa
H
Laboratory of Immunology, Faculty of Public Health, Lebanese University, Lebanon. hbtayoun@ul.edu.lb
Noun
Ghada
G
Druilhe
Pierre
P
Nakhlé
Catherine
C
Haddad
Nabil
N
Chamat
Soulaima
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C089133:merozoite surface protein 3, Plasmodium