title: Origin of bovine follicular fluid and its effect during in vitro maturation on the developmental competence of bovine oocytes.
abstract: Protein supplementation during in vitro maturation can profoundly affect both the rate and overall efficiency of the maturation procedure. The present study was conducted to assess the ability of different concentrations (1, 5, and 10%) of bovine follicular fluid (bFF) to support in vitro maturation of oocytes and subsequent developmental capacity. The bFF was derived either from competent follicles ( > 8 mm) obtained by transvaginal recovery following superovulation or from a pool of small follicles (2-5 mm) from abbatoir-derived ovaries. Bovine oocytes were cultured for 24 h in synthetic oviduct fluid medium (m-SOF) supplemented with polyvinylpyrrolidone. Following fertilization and embryo culture, more oocytes (P < 0.05) reached the blastocyst stage when oocytes were cultured with 5% bFF from competent follicles (41 +/- 3.7%) compared with bFF derived from small follicles (16 +/- 2.9%). Estradiol and recombinant human follicle stimulating hormone added to the competent bFF during maturation acted in synergy to increase blastocyst production rate (P < 0.05); this blastocyst production rate (57 +/- 1.2%) was higher than those obtained with the addition of these two hormones to bFF derived from small follicles (26 +/- 2.9%). The quality of blastocysts obtained was reflected by inner cell mass (51.30 +/- 3.5 and 25.50 +/- 3.7) and trophectoderm cell numbers (99.72 +/- 2.5 and 94.80 +/- 4.7) for bFF from competent and small follicles, respectively. In conclusion, follicular fluid originating from competent follicles increased the developmental competence of abbatoir-derived oocytes.
authors.affiliation: Department of Animal Science, Centre de Recherche en Biologie de la Reproduction, Laval University, Ste-Foy, Que., Canada G1K 7P4.
authors.forename: Atef
authors.initials: A
authors.lastname: Ali
authors.forename: Karine
authors.initials: K
authors.lastname: Coenen
authors.forename: Daniel
authors.initials: D
authors.lastname: Bousquet
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Sirard
mesh_terms: D000003:Abattoirs; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D004958:Estradiol / Q000008:administration & dosage; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology; D011994:Recombinant Proteins / Q000008:administration & dosage; D013480:Superovulation; D020858:Tissue and Organ Harvesting / Q000662:veterinary
chemical_list: D011994:Recombinant Proteins; D004958:Estradiol; D005640:Follicle Stimulating Hormone