title: [Study on the changes of IL-3 and its receptor in mice with immune-mediated aplastic anemia].
abstract: The aim of this study was to find new idea for clinical treatment of aplastic anemia. Immune-mediated aplastic anemia mice were developed, IL-3 in the supernatant with PHA stimulating splenic cells was detected by ELISA, semi-quantiting analysis of IL-3R was performed by point hybridization. The results showed that the IL-3 level in the supernatant with PHA stimulating splenic cells of immune-mediated aplastic anemia mice was higher than controls, difference between them was significant (P <0.001), while amount of IL-3 receptor by semi-quantiting analysis was lower than control significantly. In conclusion, the IL-3 receptor expression level is important for pathogenesis and treatment strategy of aplastic anemia.
authors.affiliation: Department of Hematology, The Yue Yang Iraditional Chinese and Weseten Medicine Integrated Hospital Affiliated to Shanghai Traditional Chinese University, Shanghai 200437, China. 13331993136@133sh.com
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Zhen-Qiao
authors.initials: ZQ
authors.lastname: Huang
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhou
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Xue
authors.forename: Ren-Bao
authors.initials: RB
authors.lastname: Gan
mesh_terms: D000741:Anemia, Aplastic / Q000276:immunology* / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D007377:Interleukin-3 / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis* / Q000235:genetics
chemical_list: D007377:Interleukin-3; D012333:RNA, Messenger; D016185:Receptors, Interleukin-3