Methods to study protein-protein interactions.
Protein-protein interactions are the underpinnings of a vast number of cellular processes. In recent years, the convergence of biochemistry, cellular, and molecular biology has made available a number of powerful techniques for studying such interactions. These techniques vary in their sensitivity, efficiency, and rapidity, but judicial deployment of a combination of them has proved to be effective and reliable. Here, we highlight a version of the yeast two-hybrid assay originally pioneered by Fields and Song (1989) and subsequently enhancements by other investigators. We also briefly describe a number of new fluorescent imaging-based biophysical techniques for studying protein-protein interactions FRET, FCS, and BiFC. Together, these constitute an impressive collection of tools for studying interactions among proteins.
Meng
Jin-Jun
JJ
Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinati College of Medicine, OH, USA.
Rojas
Meghan
M
Bacon
Willis
W
Stickney
John T
JT
Ip
Wallace
W
D000818:Animals; D003001:Cloning, Molecular; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D012867:Skin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D020798:Two-Hybrid System Techniques
D005456:Fluorescent Dyes