title: Large-scale cDNA transfection screening for genes related to cancer development and progression.
abstract: A large-scale assay was performed by transfecting 29,910 individual cDNA clones derived from human placenta, fetus, and normal liver tissues into human hepatoma cells and 22,926 cDNA clones into mouse NIH 3T3 cells. Based on the results of colony formation in hepatoma cells and foci formation in NIH 3T3 cells, 3,806 cDNA species (8,237 clones) were found to possess the ability of either stimulating or inhibiting cell growth. Among them, 2,836 (6,958 clones) were known genes, 372 (384 clones) were previously unrecognized genes, and 598 (895 clones) were unigenes of uncharacterized structure and function. A comprehensive analysis of the genes and the potential mechanisms for their involvement in the regulation of cell growth is provided. The genes were classified into four categories: I, genes related to the basic cellular mechanism for growth and survival; II, genes related to the cellular microenvironment; III, genes related to host-cell systemic regulation; and IV, genes of miscellaneous function. The extensive growth-regulatory activity of genes with such highly diversified functions suggests that cancer may be related to multiple levels of cellular and systemic controls. The present assay provides a direct genomewide functional screening method. It offers a better understanding of the basic machinery of oncogenesis, including previously undescribed systemic regulatory mechanisms, and also provides a tool for gene discovery with potential clinical applications.
authors.affiliation: National Laboratory for Oncogenes and Related Genes, Cancer Institute of Shanghai Jiao Tong University, 25/Ln 2200 Xie-Tu Road, Shanghai 200032, People's Republic of China.
authors.forename: Dafang
authors.initials: D
authors.lastname: Wan
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.forename: Wenxin
authors.initials: W
authors.lastname: Qin
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhou
authors.forename: Hongnian
authors.initials: H
authors.lastname: Li
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Qiu
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
authors.forename: Liping
authors.initials: L
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Genfu
authors.initials: G
authors.lastname: Yao
authors.forename: Huiqiu
authors.initials: H
authors.lastname: Jiang
authors.forename: Lianfang
authors.initials: L
authors.lastname: Qian
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.forename: Huiqun
authors.initials: H
authors.lastname: Shu
authors.forename: Xianlian
authors.initials: X
authors.lastname: Chen
authors.forename: Huili
authors.initials: H
authors.lastname: Xu
authors.forename: Minglei
authors.initials: M
authors.lastname: Guo
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Ge
authors.forename: Shengli
authors.initials: S
authors.lastname: Yang
authors.forename: Jianren
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000209:etiology / Q000235:genetics*; D009857:Oncogenes; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D014162:Transfection
chemical_list: D018076:DNA, Complementary