title: The herpes simplex virus type 1 latency-associated transcript (LAT) enhancer/rcr is hyperacetylated during latency independently of LAT transcription.
abstract: During herpes simplex virus type 1 (HSV-1) latency, only one region of the viral genome is actively transcribed: the region encoding the latency-associated transcript (LAT). A previous study demonstrated that during latency the LAT promoter is hyperacetylated at histone H3 (K9, K14) relative to lytic genes examined. In the present study, we examine the acetylation profile of regions downstream of the LAT promoter during a latent infection of murine dorsal root ganglia. These analyses revealed the following: (i) the region of the genome containing the 5' exon of the LAT primary transcript was at least as enriched in acetylated H3 as the LAT promoter, and (ii) the region of hyperacetylation does not extend to the ICP0 promoter. In order to assess the contribution of LAT transcription to the acetylation of the 5' exon region, the acetylation profile of KOS/29, a recombinant with a deletion of the LAT promoter, was examined. The region containing the 5' exon of KOS/29 was hyperacetylated relative to lytic gene regions in the absence of detectable LAT transcription. These results indicate that the region containing the 5' exon of LAT, known to contain enhancer activities and to be critical for induced reactivation (rcr), exists in a chromatin structure during latency that is distinct from other lytic gene regions. This result suggests a role for the 5' exon LAT enhancer region as a cis-acting regulator of transcription that maintains a transcriptionally permissive chromatin domain in the HSV-1 latent episome.
authors.affiliation: Department of Molecular Genetics and Microbiology, Box 100266, University of Florida College of Medicine, Gainesville, FL 32610-0266, USA.
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Kubat
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Amelio
authors.forename: Nicole V
authors.initials: NV
authors.lastname: Giordani
authors.forename: David C
authors.initials: DC
authors.lastname: Bloom
mesh_terms: D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000737:chemistry; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D005091:Exons; D005260:Female; D006657:Histones / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000235:genetics; D051379:Mice; D035683:MicroRNAs; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases; D014764:Viral Proteins / Q000235:genetics*; D014775:Virus Activation; D017735:Virus Latency
chemical_list: D002843:Chromatin; D006657:Histones; D017874:Immediate-Early Proteins; D035683:MicroRNAs; D014764:Viral Proteins; C477253:latency associated transcript, herpes simplex virus-1; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1