Dietary resistant starch alters the characteristics of colonic mucosa and exerts a protective effect on trinitrobenzene sulfonic acid-induced colitis in rats.
The protective effect of a dietary high-amylose cornstarch (HAS) against trinitrobenzene sulfonic acid (TNBS)-induced colitis was examined in rats. Rats were fed a HAS-free basal diet or, a 15% or 30% HAS supplemented diet for 10 d, and then received intracolonic TNBS to induce colitis and fed the respective diets for a further 8 d. HAS ingestion significantly protected colonic injuries as evidenced by lower colonic myeloperoxidase activity. Rats fed the HAS diet showed greater cecal short-chain fatty acid (SCFA) production than those fed the basal diet. Further, just before TNBS administration, HAS ingestion dose-dependently increased fecal and cecal mucin contents, and protein and nucleic acid contents in the colonic mucosa. HAS ingestion also reduced colonic permeability. The protective effect of HAS ingestion on TNBS-induced colitis is perhaps exerted through alterations in colonic mucosa, possibly due to cecal SCFA production.
Morita
Tatsuya
T
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. actmori@agr.shizuoka.ac.jp
Tanabe
Hiroki
H
Sugiyama
Kimio
K
Kasaoka
Seiichi
S
Kiriyama
Shuhachi
S
D000818:Animals; D002432:Cecum / Q000187:drug effects / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000627:therapeutic use; D005227:Fatty Acids / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013213:Starch / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*
D004040:Dietary Carbohydrates; D005227:Fatty Acids; D009077:Mucins; D014302:Trinitrobenzenesulfonic Acid; D013213:Starch; D009195:Peroxidase