title: Evaluation of genotoxic effect of low level 50 Hz magnetic fields on human blood cells using different cytogenetic assays.
abstract: The question whether extremely low frequency magnetic fields (ELFMFs) may contribute to mutagenesis or carcinogenesis is of current interest. In order to evaluate the possible genotoxic effects of ELFMFs, human blood cells from four donors were exposed in vitro for 48 h to 50 Hz, 1 mT uniform magnetic field generated by a Helmholtz coil system. Comet assay (SCGE), sister chromatid exchanges (SCE), chromosome aberrations (CAs), and micronucleus (MN) test were used to assess the DNA damage. ELF pretreated cells were also irradiated with 1 Gy of X-ray to investigate the possible combined effect of ELFMFs and ionizing radiation. Furthermore, nuclear division index (NDI) and proliferation index (PRI) were evaluated. Results do not evidence any DNA damage induced by ELFMF exposure or any effect on cell proliferation. Data obtained from the combined exposure to ELFMFs and ionizing radiation do not suggest any synergistic or antagonistic effect.
authors.affiliation: Section of Toxicology and Biomedical Sciences, ENEA CR Casaccia, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Testa
authors.forename: E
authors.initials: E
authors.lastname: Cordelli
authors.forename: L
authors.initials: L
authors.lastname: Stronati
authors.forename: C
authors.initials: C
authors.lastname: Marino
authors.forename: G A
authors.initials: GA
authors.lastname: Lovisolo
authors.forename: A M
authors.initials: AM
authors.lastname: Fresegna
authors.forename: D
authors.initials: D
authors.lastname: Conti
authors.forename: Paola
authors.initials: P
authors.lastname: Villani
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects; D002877:Chromosomes, Human / Q000528:radiation effects*; D020732:Cytogenetic Analysis / Q000379:methods*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D011829:Radiation Dosage; D011840:Radiation, Nonionizing; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity