title: Cell-free DNA and RNA in plasma as a new molecular marker for prostate cancer.
abstract: Extracellular nucleic acids could serve as molecular markers in the early detection of cancer and in the prediction of disease outcome. In this study we examined six molecular markers, such as: variations in the quantity of DNA in plasma, glutathione-S-transferase P1 (GSTP1) gene methylation status in plasma, carcinoembryonic antigen (CEA) and prostate-specific membrane antigen (PSMA) mRNA in peripheral blood mononuclear cells (PBMC), and plasma samples from prostate cancer patients in different stages. The combination of DNA load and GSTP1 promoter methylation status identified 83% (10/12) of the prostate cancer patients before therapy. This study shows that free circulating DNA can be detected in patients with prostate cancer compared with disease-free individuals, and suggests a new, noninvasive approach for early detection of prostate cancer.
authors.affiliation: Molecular Biology Research Center HYGEIA, Antonis Papayannis, Kiffisias Ave. & 4 Erythrou Stavrou Str., 151 23 Marousi, Athens, Hellas.
authors.forename: Eirini
authors.initials: E
authors.lastname: Papadopoulou
authors.forename: Elias
authors.initials: E
authors.lastname: Davilas
authors.forename: Vasilios
authors.initials: V
authors.lastname: Sotiriou
authors.forename: Alexander
authors.initials: A
authors.lastname: Koliopanos
authors.forename: Filipos
authors.initials: F
authors.lastname: Aggelakis
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Dardoufas
authors.forename: Niki J
authors.initials: NJ
authors.lastname: Agnanti
authors.forename: Irini
authors.initials: I
authors.lastname: Karydas
authors.forename: Georgios
authors.initials: G
authors.lastname: Nasioulas
mesh_terms: D002474:Cell-Free System / Q000378:metabolism*; D004247:DNA / Q000097:blood* / Q000235:genetics; D005819:Genetic Markers / Q000502:physiology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood* / Q000235:genetics; D012313:RNA / Q000097:blood* / Q000235:genetics; D018709:Statistics, Nonparametric
chemical_list: D005819:Genetic Markers; D012313:RNA; D004247:DNA