title: Round-robin comparison of methods for the detection of human enteric viruses in lettuce.
abstract: Five methods that detect human enteric virus contamination in lettuce were compared. To mimic multiple contaminations as observed after sewage contamination, artificial contamination was with human calicivirus and poliovirus and animal calicivirus strains at different concentrations. Nucleic acid extractions were done at the same time in the same laboratory to reduce assay-to-assay variability. Results showed that the two critical steps are the washing step and removal of inhibitors. The more reliable methods (sensitivity, simplicity, low cost) included an elution/concentration step and a commercial kit. Such development of sensitive methods for viral detection in foods other than shellfish is important to improve food safety.
authors.affiliation: IFREMER, Laboratoire de Microbiologie, BP 21105, 44311 Nantes cedex 03, France. sleguyad@ifremer.fr
authors.forename: Françoise S
authors.initials: FS
authors.lastname: Le Guyader
authors.forename: Anna-Charlotte
authors.initials: AC
authors.lastname: Schultz
authors.forename: Larissa
authors.initials: L
authors.lastname: Haugarreau
authors.forename: Luciana
authors.initials: L
authors.lastname: Croci
authors.forename: Leena
authors.initials: L
authors.lastname: Maunula
authors.forename: Erwin
authors.initials: E
authors.lastname: Duizer
authors.forename: Froukje
authors.initials: F
authors.lastname: Lodder-Verschoor
authors.forename: Carl-Henrik
authors.initials: CH
authors.lastname: von Bonsdorff
authors.forename: Elizabetha
authors.initials: E
authors.lastname: Suffredini
authors.forename: Wim M M
authors.initials: WM
authors.lastname: van der Poel
authors.forename: Rosanna
authors.initials: R
authors.lastname: Reymundo
authors.forename: Marion
authors.initials: M
authors.lastname: Koopmans
mesh_terms: D002139:Caliciviridae / Q000302:isolation & purification*; D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D018545:Lactuca / Q000821:virology*; D009693:Nucleic Acid Hybridization; D017955:Poliovirus / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral; D011092:Polyethylene Glycols