Beta-adrenergic receptor density and adenylate cyclase activity in lead-exposed rat brain after cessation of lead exposure.
To understanding the reversible or irreversible harm to the beta-adrenergic system in the brain of lead-exposed rats, this study sets up an animal model to estimate the change in the sympathetic nervous system of brain after lead exposure was withdrawn. We address the following topics in this study: (a) the relationship between withdrawal time of lead exposure and brain beta-adrenergic receptor, blood lead level, and brain lead level in lead-exposed rats after lead exposure was stopped; and (b) the relationship between lead level and beta-adrenergic receptor and cyclic AMP (c-AMP) in brain. Wistar rats were chronically fed with 2% lead acetate and water for 2 months. Radioligand binding was assayed by a method that fulfilled strict criteria of beta-adrenergic receptor using the ligand [125I]iodocyanopindolol. The levels of lead were determined by electrothermal atomic absorption spectrometry. The c-AMP level was determined by radioimmunoassay. The results showed a close relationship between decreasing lead levels and increasing numbers of brain beta-adrenergic receptors and brain adenylate cyclase activity after lead exposure was withdrawn. The effect of lead exposure on the beta-adrenergic system of the brain is a partly reversible condition.
Chang
Huoy-Rou
HR
Department of Biomedical Engineering, I-Shou University, 1, Section 1, Shiuecheng Road, Dashu Shiang, Kaohsiung County, Taiwan. huoy@isu.edu.tw
Tsao
Der-An
DA
Yu
Hsin-Su
HS
Ho
Chi-Kung
CK
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004195:Disease Models, Animal; D020120:Iodocyanopindolol / Q000494:pharmacology; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity*; D007855:Lead Poisoning / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta; D007854:Lead; D020120:Iodocyanopindolol; D000242:Cyclic AMP; D000262:Adenylyl Cyclases