Nitric oxide-scavenging activity of polyhydroxylated fullerenol, C60(OH)24.
Investigation of the possible nitric oxide-scavenging activity of hydroxylated derivative of fullerene, fullerenol C60(OH)24, demonstrated that it expressed direct scavenging activity toward nitric oxide radical (NO) liberated within solution of sodium nitroprusside (SNP), a well known NO donor. In parallel, pre-treatment (30') with intratesticular injection of fullerenol (60 microg/each testis) prevented NO-induced decrease of catalase, glutathione transferase and glutathione peroxidase activities in the denucleated fraction of interstitial testicular cells of adult rats 2 h after intratesticular injection of SNP (20 microg/each testis). In addition, fullerenol decreased formation of thiobarbituric acid-reactive substances (TBA-RS) with similar efficiency as butylated hydroxy toluen (BHT), a well known antioxidant. Also, fullerenol expressed certain scavenging activity toward superoxide anion (O2-) in xanthine/xanthine oxidase system. In summary, results obtained in this study confirmed free radical-scavenging activity of fullerenol, and according to our knowledge, it is the first evidence of direct NO-quenching activity of hydroxylated C60 derivative in different milieu.
Mirkov
Snezana M
SM
Department of Medicine, The University of Chicago, 5841 South Maryland Ave. MC 2115, Chicago, IL 60637, USA.
Djordjevic
Aleksandar N
AN
Andric
Nebojsa L
NL
Andric
Silvana A
SA
Kostic
Tatjana S
TS
Bogdanovic
Gordana M
GM
Vojinovic-Miloradov
Mirjana B
MB
Kovacevic
Radmila Z
RZ
D000818:Animals; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology*; D037741:Fullerenes / Q000008:administration & dosage / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000008:administration & dosage; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D013737:Testis / Q000166:cytology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D016166:Free Radical Scavengers; D037741:Fullerenes; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D009599:Nitroprusside; C108127:fullerenol; D009569:Nitric Oxide; D010088:Oxidoreductases; D005982:Glutathione Transferase