A practical method for measuring deoxynivalenol, nivalenol, and T-2 + HT-2 toxin in foods by an enzyme-linked immunosorbent assay using monoclonal antibodies.
We have developed and tested an enzyme-linked immunosorbent assay system for individual measurement of deoxynivalenol, nivalenol, and T-2 + HT-2 toxin using monoclonal antibodies for 3,4,15-triacetyl-nivalenol, for both 3,4,15-triacetyl-nivalenol and 3,15-diacetyl-deoxynivalenol, and for acetyl-T-2 toxin. The assay system comprised three kits (desinated the DON + NIV kit, the NIV kit, and the T-2 + HT-2 kit). The practical performance of the enzyme-linked immunosorbent assay system was assessed by assaying trichothecene mycotoxins in wheat kernels. The enzyme-linked immunosorbent assay system meets all the requirements for use in a routine assay in terms of sensitivity (detection limit: deoxynivalenol 80 ng/g, nivalenol 80 ng/g, T-2 toxin 30 ng/g), reproducibility (total coefficient of variation: 1.9-6.2%), accuracy (recovery: 93.8-112.0%), simplicity and rapidity (time required: <2 h), mass handling (>42 samples/assay), and a good correlation with gas chromatography-mass spectrometry (r=0.9146-0.9991). Components derived from the wheat extract did not interfere with the assay kits. The enzyme-linked immunosorbent assay system is a useful alternative method to gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, or liquid chromatography-ultraviolet absorption for screening cereals and foods for trichothecene mycotoxin contamination.
Yoshizawa
Takumi
T
Department of Biochemistry and Food Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
Kohno
Hiroaki
H
Ikeda
Kazuyuki
K
Shinoda
Tatsuya
T
Yokohama
Hiroaki
H
Morita
Kazuki
K
Kusada
Osamu
O
Kobayashi
Yoshinori
Y
D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005433:Flour / Q000032:analysis*; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology; D014908:Triticum / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D014255:Trichothecenes; C038405:nivalenol; D013605:T-2 Toxin; C007262:deoxynivalenol; C012351:HT-2 toxin