title: Aquaporin-1 expressed in cultured human trabecular meshwork cells.
abstract: OBJECTIVE
  To determine if aquaporin-1 could be detected in cultures of human trabecular meshwork cells.
  
  
  METHODS
  Using primers specific for aquaporin-1, reverse transcription combined with polymerase chain reaction (RT-PCR) yielded a product and its size with total RNA prepared from the human trabecular meshwork cells. SDS-PAGE and immunoblotting were also used in this study to detect the specific water channel.
  
  
  RESULTS
  The presence of this product and its size (298 base pairs) are consistent with that of an aquaporin-1 message in these cells. A band of 28 kD in agreement with the molecular size of aquaporin-1 was showed in a film by immunoblotting.
  
  
  CONCLUSIONS
  The presence of aquaporin-1 in human trabecular meshwork cells, the predominant cell-type of the primary outflow region of the human eye, suggests that water channels may be involved in the movement of aqueous fluid out of the eye. In addition, the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides an in vitro model to study the endogenous expression of aquaporin-1 and its possible role in the regulation of aqueous outflow.
authors.affiliation: Zhongshan Ophthalmic center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Mingkai
authors.initials: M
authors.lastname: Lin
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Yehong
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Lan
authors.forename: Keming
authors.initials: K
authors.lastname: Yu
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D051398:Aquaporin 1 / Q000096:biosynthesis*; D002478:Cells, Cultured; D006801:Humans; D015151:Immunoblotting; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014129:Trabecular Meshwork / Q000166:cytology* / Q000378:metabolism
chemical_list: D051398:Aquaporin 1; D012313:RNA