title: Activity-dependent transcription regulation of PSD-95 by neuregulin-1 and Eos.
abstract: Neuregulin-1 (Nrg-1) contains an intracellular domain (Nrg-ICD) that translocates into the nucleus, where it may regulate gene expression upon neuronal depolarization. However, the identity of its target promoters and the mechanisms by which it regulates transcription have been elusive. Here we report that, in the mouse cochlea, synaptic activity increases the level of nuclear Nrg-ICD and upregulates postsynaptic density protein-95 (PSD-95), a scaffolding protein that is enriched in post-synaptic structures. Nrg-ICD enhances the transcriptional activity of the PSD-95 promoter by binding to a zinc-finger transcription factor, Eos. The Nrg-ICD-Eos complex induces endogenous PSD-95 expression in vivo through a signaling pathway that is mostly independent of gamma-secretase regulation. This upregulation of PSD-95 expression by the Nrg-ICD-Eos complex provides a molecular basis for activity-dependent synaptic plasticity.
authors.affiliation: Department of Otolaryngology, Washington University, St. Louis, Missouri 63110, USA. jbao@wustl.edu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Bao
authors.forename: Hana
authors.initials: H
authors.lastname: Lin
authors.forename: Yannan
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Debin
authors.initials: D
authors.lastname: Lei
authors.forename: Abdullah
authors.initials: A
authors.lastname: Osman
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Penggao
authors.initials: P
authors.lastname: Dai
authors.forename: Kevin K
authors.initials: KK
authors.lastname: Ohlemiller
authors.forename: Richard T
authors.initials: RT
authors.lastname: Ambron
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D003051:Cochlea / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000502:physiology; D000076122:Disks Large Homolog 4 Protein; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005110:Extracellular Space / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D017930:Genes, Reporter / Q000502:physiology; D051528:Guanylate Kinases; D006801:Humans; D051740:Ikaros Transcription Factor; D007150:Immunohistochemistry / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051379:Mice; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D020890:Neuregulin-1 / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011189:Potassium Chloride / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction / Q000502:physiology; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D014162:Transfection / Q000379:methods
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; C497239:IKZF1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D012333:RNA, Messenger; D014157:Transcription Factors; C118760:Zfpn1a4 protein, mouse; C033652:postsynaptic density proteins; D051740:Ikaros Transcription Factor; D011189:Potassium Chloride; D051528:Guanylate Kinases