title: Crystallization and preliminary X-ray crystallographic studies of a lectin from the mushroom Marasmius oreades.
abstract: The Marasmius oreades agglutinin (MOA) recognizes blood group B oligosaccharides. This mushroom lectin belongs to the ricin superfamily and is currently the only lectin known with exclusive specificity for Galalpha1,3Gal-structures, as occur in the subterminally fucosylated blood group B epitope Galalpha1,3(Fucalpha1,2)Galbeta1,4GlcNAc (MOA's preferred ligand) or without fucosylation in the xenotransplantation epitope. MOA has been co-crystallized with the linear blood group B trisaccharide Galalpha1,3Galbeta1,4GlcNAc using the hanging-drop vapour-diffusion technique at room temperature. MOA crystals were grown in the presence of ammonium formate and HEPES buffer. A 3.0 A data set has been collected. Preliminary analysis of the X-ray data is consistent with space group P3(1) or P3(2) and unit-cell parameters a = b = 105, c = 113 A, with two dimers per asymmetric unit.
authors.affiliation: Department of Chemistry and Bioscience, Chalmers University of Technology, PO Box 462, SE-405 30 Göteborg, Sweden.
authors.forename: Elin
authors.initials: E
authors.lastname: Grahn
authors.forename: Asa
authors.initials: A
authors.lastname: Holmner
authors.forename: Cecilia
authors.initials: C
authors.lastname: Cronet
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tateno
authors.forename: Harry C
authors.initials: HC
authors.lastname: Winter
authors.forename: Irwin J
authors.initials: IJ
authors.lastname: Goldstein
authors.forename: Ute
authors.initials: U
authors.lastname: Krengel
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D037102:Lectins / Q000737:chemistry*
chemical_list: D037102:Lectins