Automated multi-well device to measure transepithelial electrical resistances under physiological conditions.
Measurement of transendothelial or transepithelial electrical resistances (TERs) is a straightforward in situ experimental approach to monitor the expression or modulation of barrier-forming cell-to-cell contacts (tight junctions) in cultured cells grown on porous filters. Although widely accepted, there is currently no device available to automatically measure the time course of TERs under ordinary cell culture conditions (37 degrees C, 5% or 10% CO2). This paper describes a development from our laboratory that is capable of following in parallel the TERs of several filter-grown cell layers with time and in an entirely computer-controlled fashion. The cell cultures can be followed even in long-term experiments without any manual assistance or opening of the incubator Besides reading TER values, this approach also returns the electrical capacitance of the cell layers, which is indicative of the expression of microvilli and other membrane extrusions. The device is based on reading the frequencydependent impedance of the cell layer, followed by equivalent circuit modeling to extract the cell-related parameters. It is compatible with several multi-well formats (up to 96 wells) and controlled by custom-designed software that reads, analyzes, and presents the data.
Wegener
Joachim
J
Institüt für Biochemie, Westfälische Wilhelms-Universität Münster, Münster, Germany. wegenej@uni-muenster.de
Abrams
Dimitri
D
Willenbrink
Wolfgang
W
Galla
Hans-Joachim
HJ
Janshoff
Andreas
A
D000818:Animals; D002199:Capillary Permeability / Q000502:physiology*; D002478:Cells, Cultured; D025802:Electric Capacitance; D004553:Electric Conductivity; D017097:Electric Impedance; D042783:Endothelial Cells / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D013552:Swine; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000295:instrumentation