title: Effects of trichloroethylene and perchloroethylene on muscle contractile responses and epithelial prostaglandin release and acetylcholinesterase activity in swine trachea.
abstract: Trichloroethylene (TCE) and perchloroethylene (PERC) have been reported to induce respiratory complications such as airway hyperactivity and asthma. The present study was designed to investigate their influence on smooth muscle contraction and epithelial release of prostanoids in swine trachea. Results showed that TCE and PERC exposure did not alter the basal tone of tracheal smooth muscle. However, TCE and PERC concentration-dependently increased both ACh-induced and high K+-induced muscle contraction. In addition to potentiation of muscle contractile responses evoked by acetylcholine or histamine, pretreatment of smooth muscle with PERC at higher concentration significantly suppressed the relaxant activity of beta-adrenergic agonists. The epithelial prostaglandin (PG)E2, but not PGD2, release from tracheal epithelium was significantly increased by TCE and PERC. In addition, the acetylcholinesterse (AChE) activity of tracheal epithelia was reduced by TCE and PERC. In conclusion, our results suggest that the enhancement of spasmogen-evoked muscle contractile responses and epithelial PGE2 secretion, as well as reduction of epithelial AChE activity, may participate in airway impairment and hyperresponsiveness after TCE and PERC exposure.
authors.affiliation: Institute of Pharmacology and Toxicology, Tzu Chi University No 701, Section 3, Hualien 970, Taiwan.
authors.forename: Hwei-Hsien
authors.initials: HH
authors.lastname: Chen
authors.forename: Yi-Ruu
authors.initials: YR
authors.lastname: Lin
authors.forename: Quan-Guo
authors.initials: QG
authors.lastname: Peng
authors.forename: Ming-Huan
authors.initials: MH
authors.lastname: Chan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects*; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects; D015230:Prostaglandin D2 / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013552:Swine; D013750:Tetrachloroethylene / Q000633:toxicity; D014132:Trachea / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D014241:Trichloroethylene / Q000633:toxicity
chemical_list: D004785:Environmental Pollutants; D011453:Prostaglandins; D014241:Trichloroethylene; D000110:Acetylcholinesterase; D015232:Dinoprostone; D015230:Prostaglandin D2; D013750:Tetrachloroethylene