title: Insulin degrading enzyme is expressed in the human cerebrovascular endothelium and in cultured human cerebrovascular endothelial cells.
abstract: Insulin degrading enzyme (IDE) is found in the cytosol, peroxisomes and plasma membrane of many cells. Although it preferentially cleaves insulin it can also cleave many other small proteins with diverse sequences including the monomeric form of the amyloid beta peptide (A beta). In the brain, IDE has been reported to be expressed predominantly in neurons. In this study, IDE expression was detected in cultured human cerebrovascular endothelial cells. Using laser capture microdissection followed by PCR analysis, it was found that IDE mRNA is expressed in human brain blood vessels. Using immunofluorescence and multiphoton microscopy IDE was localized to the endothelium of the cerebrovascular blood vessels in human.
authors.affiliation: ENR VA Medical Center, Bedford, MA 01730, USA.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Gao
authors.forename: Patricia B
authors.initials: PB
authors.lastname: Eisenhauer
authors.forename: Kelly
authors.initials: K
authors.lastname: Conn
authors.forename: John A
authors.initials: JA
authors.lastname: Lynch
authors.forename: John M
authors.initials: JM
authors.lastname: Wells
authors.forename: M David
authors.initials: MD
authors.lastname: Ullman
authors.forename: Ann
authors.initials: A
authors.lastname: McKee
authors.forename: Hamant S
authors.initials: HS
authors.lastname: Thatte
authors.forename: Richard E
authors.initials: RE
authors.lastname: Fine
mesh_terms: D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007339:Insulysin / Q000235:genetics* / Q000378:metabolism*; D008833:Microcirculation; D013378:Substantia Nigra / Q000098:blood supply
chemical_list: D007339:Insulysin