title: Positive predictive value of Gen-Probe APTIMA Combo 2 testing for Neisseria gonorrhoeae in a population of women with low prevalence of N. gonorrhoeae infection.
abstract: We collected specimens from women who tested positive for Neisseria gonorrhoeae using the Gen-Probe APTIMA Combo 2 (AC2), and we performed confirmatory tests using a nucleic acid amplification test (NAAT) that targets alternate gonococcal nucleic acid sequences. Among 59,664 specimens, 280 (0.47%) had positive results using AC2; 265 of these specimens were tested using the confirmatory NAAT, of which 258 yielded positive results (positive predictive value, 97.4%; 95% confidence interval, 95.1%-98.8%). Routine confirmatory testing of specimens with positive AC2 gonorrhea test results is not indicated.
authors.affiliation: Division of Infectious Diseases, University of Washington, Seattle, WA, USA. golden@u.washington.edu
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Golden
authors.forename: James P
authors.initials: JP
authors.lastname: Hughes
authors.forename: Linda E
authors.initials: LE
authors.lastname: Cles
authors.forename: Karen
authors.initials: K
authors.lastname: Crouse
authors.forename: Katherine
authors.initials: K
authors.lastname: Gudgel
authors.forename: Jinxin
authors.initials: J
authors.lastname: Hu
authors.forename: Paul D
authors.initials: PD
authors.lastname: Swenson
authors.forename: Walter E
authors.initials: WE
authors.lastname: Stamm
authors.forename: H Hunter
authors.initials: HH
authors.lastname: Handsfield
mesh_terms: D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D015995:Prevalence; D012680:Sensitivity and Specificity