[Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells].
OBJECTIVE
To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G(1)/S-phase renal tubule epithelial cells (RTECs).


METHODS
G1-phase RTECs were divided into cantharidin-treated group, exposed to sodium cyanide (CN) and cantharidin, hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography.


RESULTS
The concentration of CN exposure for 1 h, ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50+/-0.26 mmol/g protein, 4.25+/-0.11 mmol/g protein, 8.58+/-0.13 mmol/g protein, respectively, P<0.01).


CONCLUSIONS
Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.
Shen
Qing
Q
Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mailsq@fmmu.edu.cn
Yao
Yu-jia
YJ
Li
Ru-ying
RY
Ding
Cui-ling
CL
Hou
Li-chao
LC
Sun
Xu-de
XD
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002193:Cantharidin / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D016193:G1 Phase; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D016196:S Phase; D013552:Swine
D004791:Enzyme Inhibitors; D000255:Adenosine Triphosphate; D002193:Cantharidin