title: Polycyclic aromatic hydrocarbons storage by Fusarium solani in intracellular lipid vesicles.
abstract: Accumulation and elimination of polycyclic aromatic hydrocarbons (PAHs) were studied in the fungus Fusarium solani. When the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of F. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: Sudan III and Rhodamine B. The fluorescence produced by Rhodamine B and PAH benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that F. solani stored PAH in pre-existing lipid vesicles. A passive temperature-independent process is involved in the benzo[a]pyrene uptake and storage. Sodium azide, a cytochrome c oxidation inhibitor, and the two cytoskeleton inhibitors colchicine and cytochalasin did not prevent the transport and accumulation of PAH in lipid vesicles of F. solani hyphae. F. solani degraded a large range of PAHs at different rates. PAH intracellular storage in lipid vesicles was not necessarily accompanied by degradation and was common to numerous other fungi.
authors.affiliation: Laboratoire de Mycologie/Phytopathologie/Environnement, Université du Littoral-Côte d'Opale, 17 avenue Blériot, BP 699, 62228 Calais Cedex, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Verdin
authors.forename: Anissa
authors.initials: A
authors.lastname: Lounès-Hadj Sahraoui
authors.forename: Ray
authors.initials: R
authors.lastname: Newsam
authors.forename: Gary
authors.initials: G
authors.lastname: Robinson
authors.forename: Roger
authors.initials: R
authors.lastname: Durand
mesh_terms: D001391:Azo Compounds / Q000032:analysis; D001673:Biodegradation, Environmental; D001692:Biological Transport / Q000187:drug effects; D003470:Culture Media; D003599:Cytoskeleton / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D050356:Lipid Metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics*; D013696:Temperature
chemical_list: D001391:Azo Compounds; D003470:Culture Media; D011084:Polycyclic Aromatic Hydrocarbons; D005947:Glucose; C033006:sudan III