[Effect of methylmercury on expression of immediate early gene c-jun mRNA in rat brain].
In order to probe into the early prediction molecular index and the signal transduction molecular mechanism of methyl mercury chloride (MMC) neurotoxicity, the expression of c-jun mRNA in rat brains induced by different concentration MMC for different times were observed by using reverse transcription polymerase chain reaction (RT-PCR) methods (the control group was physiological saline of 0.9%, the concentrations of expose groups were 0.05, 0.5, 5 mg x kg(-1) respectively, the sampling times were 20, 60, 240, 1440 min). The result showed the expression of c-jun mRNA in rat brains was prior to the accumulation of mercury, and the expression of c-jun mRNA in rat brains could early predict the neurotoxicity of MMC. IEG (c-jun) participated in the toxicity process of injury by MMC.
Cheng
Jin-ping
JP
School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China.
Wang
Wen-hua
WH
Qu
Li-ya
LY
Jia
Jin-ping
JP
Zheng
Min
M
Lin
Zhi-xin
ZX
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D017781:Genes, Immediate-Early / Q000235:genetics*; D008297:Male; D008767:Methylmercury Compounds / Q000633:toxicity*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D008767:Methylmercury Compounds; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; C004925:methylmercuric chloride