title: Comparison between sodium dodecylsulphate and cetyltrimethylammonium bromide as mobile phases in the micellar liquid chromatography determination of non-steroidal anti-inflammatory drugs in pharmaceuticals.
abstract: The retention behaviour of non-steroidal anti-inflammatory drugs (NSAIDs) using micellar mobile phases of sodium dodecylsulphate (SDS) is studied and compared with that observed with micellar mobile phases of cetyltrimethylammonium bromide (CTAB). A liquid chromatographic procedure for the determination of acemetacin, diclofenac, indomethacin, ketoprofen, naproxen and tolmetin in pharmaceutical preparations is described. The proposed system uses a Kromasil C18 analytical column and a solution of 0.15 M SDS at pH 3 with 10% 1-propanol as mobile phase. Under these conditions, the studied NSAIDs elute between 6 and 10 min at a 1 mL min(-1) flow rate. Limits of detection (LOD) are lower than 0.5 microg mL(-1). Recoveries in the analysis of the pharmaceutical preparations are ranged between 91 and 104% respect to the nominal content declared by the manufacturers and the relative standard deviations are in general lower than 4%.
authors.affiliation: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València,C/Vicente Andrés Estellés s/n, E-46100 Burjassot, Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Martínez-Algaba
authors.forename: L
authors.initials: L
authors.lastname: Escuder-Gilabert
authors.forename: S
authors.initials: S
authors.lastname: Sagrado
authors.forename: R M
authors.initials: RM
authors.lastname: Villanueva-Camañas
authors.forename: M J
authors.initials: MJ
authors.lastname: Medina-Hernández
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002138:Calibration; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary; D007202:Indicators and Reagents; D012015:Reference Standards; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002593:Cetrimonium Compounds; D007202:Indicators and Reagents; D012996:Solutions; D012967:Sodium Dodecyl Sulfate; D000077286:Cetrimonium