Pre-B-cell colony formation assay.
Latent membrane protein 2A (LMP-2A) of Epstein-Barr virus (EBV) mimics a constitutively active B-cell receptor (BCR) and plays a key role in viral latency and EBV pathogenesis. By functioning as a BCR mimic, LMP-2A drives B-cell development, resulting in the bypass of normal B-cell developmental checkpoints. To assess the function of LMP-2A, we have utilized a colony formation assay for the progenitor B cells that uses the B-cell proliferation factor IL-7.
Ikeda
Masato
M
Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Longnecker
Richard
R
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D015851:Interleukin-7; D051379:Mice; D014763:Viral Matrix Proteins
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D015851:Interleukin-7; D014763:Viral Matrix Proteins