Novel mutations within the embB gene in ethambutol-susceptible clinical isolates of Mycobacterium tuberculosis.
Genetic analysis of the embB gene revealed mutations in 17 (68%) of 25 ethambutol (EMB) resistant isolates (M306I, M306V, M306L, Q497R) but also in 4 (20%) of 20 EMB-susceptible isolates of Mycobacterium tuberculosis, namely, an ATG-->ATM substitution resulting in M306I, G406N, and the novel alterations M423I and A659T.
Lee
Ann S G
AS
Division of Medical Sciences, National Cancer Centre, Singapore 169610, Republic of Singapore. dmslsg@nccs.com.sg
Othman
Siti Noor Khadijah
SN
Ho
Yu Min
YM
Wong
Sin Yew
SY
D000995:Antitubercular Agents / Q000494:pharmacology*; D003062:Codon / Q000235:genetics; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004977:Ethambutol / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012846:Singapore / Q000453:epidemiology
D000995:Antitubercular Agents; D003062:Codon; D017931:DNA Primers; D004269:DNA, Bacterial; D004977:Ethambutol