title: The tetrameric molecule of conventional kinesin contains identical light chains.
abstract: Conventional kinesin is a multifunctional motor protein that transports numerous organelles along microtubules. The specificity of kinesin-cargo binding is thought to depend on the type(s) of light chains that a kinesin molecule contains. We have shown previously that different isoforms of kinesin light chains are associated with different types of cargo, mitochondria and membranes of the Golgi complex. Here, we provide evidence that the two light chains present within each kinesin molecule are always of the same type. Further, we demonstrate that kinesin heavy chains interact with nascent light-chain polypeptides on ribosomes. These data suggest that incorporation of the two identical light chains into a single kinesin molecule most likely occurs cotranslationally.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, Moscow 117334, Russian Federation. fatima@eimb.ru
authors.forename: Fatima K
authors.initials: FK
authors.lastname: Gyoeva
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Sarkisov
authors.forename: Alexey L
authors.initials: AL
authors.lastname: Khodjakov
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Minin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D019281:Dimerization; D006801:Humans; D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011132:Polyribosomes / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D010455:Peptides; D020033:Protein Isoforms; D016547:Kinesins