CDNA cloning and expression of ATP synthase subunit b from the fire-bellied frog Bombina orientalis (Anura: Discoglossidae).
We cloned the Bombina orientalis adenosine triphosphate (ATP) synthase subunit b gene from a B. orientalis oviduct cDNA library. The transcript was 997 bp long and encoded 250 amino acid residues. It showed high similarity to amphibian (84-85%), mammalian (56-62%) and invertebrate (46-50%) sequences. In phylogenetic analyses, it clustered with other amphibian sequences. This gene was highly expressed in brain, intestine and oviduct but not in muscle and liver. In this paper, we report the basic characteristics of B. orientalis ATP synthase subunit b gene.
Kim
Min-Sun
MS
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
Kim
Il-Chan
IC
Kim
Sang-Soo
SS
Kim
Sook-Kyoung
SK
Lee
Young-Mi
YM
Kang
Han Seung
HS
Kweon
Hee-Seok
HS
Hwang
Ui-Wook
UW
Kim
Moon Kyoo
MK
Lee
Jae-Seong
JS
Yoon
Yong-Dal
YD
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010057:Oviducts / Q000378:metabolism*; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D017931:DNA Primers; D018076:DNA, Complementary; D006180:Proton-Translocating ATPases