[Studies on the effects of Siwu Mixture on reversing multidrug resistance of human erythrocyte leukemic cell line K562/ADM].
OBJECTIVE
To study the effects of Siwu Mixture on reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM.


METHODS
With verapamil set as a positive control, MTT assay was applied to evaluate the resistant factor of cell line K562/ADM to anticancer drugs and the reversal index of Siwu Mixture on MDR of the cells. The concentration of adriamycin(ADM) in the cells was measured by reversal-phase high performance liquid chromatography. The immunofluorescence method was used to determine the expression of P-glucoprotein(Pgp) on cell membrane.


RESULTS
When ADM was combined with Siwu Mixture, its concentration in the cells and the reversal index of MDR were significantly higher than those of blank control (P < 0.01). However, the expression of Pgp on cell membrane showed little difference ( P > 0.05).


CONCLUSIONS
In case of noncytotoxic dose, Siwu Mixture showed significant effect on reversing MDR of cell line K562/ADM. The effect might be related to decreasing efflux of anticancer drugs in the cells and increasing their concentration in the cells. Nevertheless, it had no influence on the expression of Pgp on cell membrane.
Xia
Lei
L
Department of Chinese Science and Engineering, College of Pharmaceutical Sciences, Zhejiong University, Hangzhou 310027, China.
Shen
Peng
P
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D029971:Angelica sinensis / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology; D004338:Drug Combinations; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D030004:Ligusticum / Q000737:chemistry; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004317:Doxorubicin