[Using thioGlo-1 for the determination of thiols in brain tissue].
A technique for the fluorometric determination of protein and non-protein thiols in brain tissue with the maleimide reagent ThioGlo-1is has been described. Comparison with the most popular spectrophotometric Ellman method has been performed. The ThioGlo-1-based method is highly sensitive and may be used for thiol determination both in homogenates and supernatants of brain tissue.
Iakovlev
A A
AA
Guliaeva
N V
NV
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005470:Fluorometry; D008297:Male; D009281:Naphthalenes / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D009281:Naphthalenes; D011758:Pyrroles; D013438:Sulfhydryl Compounds; C498335:ThioGlo-1