A method to extract algae toxin of microcystin-LR.
A simple and low-cost method to obtain cyanobacterial toxin microcystin-LR (MC-LR) was developed. A new strain of Microcystis aeruginosa, named DC-1, producing microcystin-LR but not microcystin-RR, was separated from the field blooming algae samples of Dianchi Lake, in southwest of China. Following three times' freeze and thaw treatment, the cultivated DC-1 cells were extracted with 40% methanol in water. The extract was centrifuged and the supernatant applied to a Hydrophilic-Lipophilic Balance (HLB) SPE cartridge. Eluted impurities with a certain gradient from 30% to 50% methanol in water, MC-LR was finally eluted from the HLB cartridge with 60% methanol in water, and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR samples may be used in adsorption and biodegradation experiments instead of using expensive standard reagents.
Zhang
Ming-ming
MM
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Pan
Gang
G
Yan
Hai
H
Chen
Hao
H
D001427:Bacterial Toxins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D000458:Cyanobacteria; D004791:Enzyme Inhibitors / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors
D001427:Bacterial Toxins; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D010749:Phosphoprotein Phosphatases; C057862:cyanoginosin LR