Total proteolytic activity and content of the main proteinase inhibitors in blood plasma of rats bearing doxorubicin-sensitive and doxorubicin-resistant Guerin carcinoma.
The AIM of work was to evaluate the alteration of the total proteolytic activity (TPA) and the levels of alpha(1)-proteinase inhibitor (alpha1PI) and alpha(2)-macroglobuline (alpha2M) in blood plasma of rats bearing Guerin carcinoma upon the development of Doxorubicin (DOX) resistance.


METHODS
TPA and alpha1PI and alpha2M content in the blood plasma of male Wistar rats bearing DOX-resistant and DOX-sensitive Guerin carcinoma were evaluated by standard biochemical methods.


RESULTS
During growth of both DOX-sensitive and DOX-resistant Guerin carcinoma, TPA decrease in blood plasma and the increase of alpha1PI levels were registered; in DOX-resistant group this effect was more pronounced. Alpha2M content in blood plasma of animals from both experimental groups was considerably smaller than that of the control and was the lowest in DOX-resistant group.


CONCLUSIONS
The growth of DOX-resistant Guerin carcinoma is accompanied by imbalance of proteolysis processes in the blood plasma, particularly, alteration of TPA and alpha1PI and alpha2M levels.
Chekhun
Vasyl F
VF
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
Kovtonyuk
Oksana V
OV
Todor
Igor N
IN
Kulik
Galina I
GI
Tryndiak
Volodymyr P
VP
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use; D002277:Carcinoma / Q000097:blood / Q000188:drug therapy / Q000201:enzymology*; D004317:Doxorubicin / Q000494:pharmacology* / Q000627:therapeutic use; D004351:Drug Resistance; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood*; D011480:Protease Inhibitors / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D000903:Antibiotics, Antineoplastic; D009363:Neoplasm Proteins; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D004317:Doxorubicin; D012697:Serine Endopeptidases