Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism.
Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.
Franceschetti
Marina
M
Division of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK.
Perry
Barry
B
Thompson
Benjamin
B
Hanfrey
Colin
C
Michael
Anthony J
AJ
D000064:Acclimatization; D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D020543:Proteome; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D010446:Peptide Fragments; D010940:Plant Proteins; D011073:Polyamines; D020543:Proteome