Detection and identification of phenazone-type drugs and their microbial metabolites in ground and drinking water applying solid-phase extraction and gas chromatography with mass spectrometric detection.
A new analytical method applying in situ derivatization was developed to enable the extraction of polar drug metabolites from water samples by solid-phase extraction (SPE). An additional derivatization by silylation was used to enhance the sensitivity of analyte detection by gas chromatography-mass spectrometry (GC-MS). Thus, the two metabolites 1,5-di-methyl-1,2-dehydro-3-pyrazolone (DP) and 4-(2-methylethyl)-1,5-dimethyl-1,2-dehydro-3-pyrazolone (PDP), postulated for the degradation of phenazone and propyphenazone, were identified and detected up to the microg/L level in raw and drinking water samples from public water supply.
Zühlke
Sebastian
S
Institute of Food Chemistry, Technical University Berlin, Sekr TIB 4/3-1, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
Dünnbier
Uwe
U
Heberer
Thomas
T
D000107:Acetylation; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000378:metabolism; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005858:Germany; D012015:Reference Standards; D013048:Specimen Handling; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; C009077:propyphenazone; D000983:Antipyrine