title: [The study of immunofluorescence on the retinal digest preparations].
abstract: OBJECTIVE
  To study the technique of immunofluorescence staining on the digested retina preparations.
  
  
  METHODS
  Retinas of 5 normal Wistar rats and 5 STZ diabetic Wistar rats of were used to make digested retina preparations and carry out immunofluorescence staining with the vascular cell adhesion molecule-1 (VCAM-1).
  
  
  RESULTS
  The retinal vessels were clearly observed; There were a lot of positive cells in the retinal preparations of the diabetic Wistar rats, while there were no positive cells in the retinal preparations of the normal rats.
  
  
  CONCLUSIONS
  It is feasible to carry out immunofluorescence staining on the digested retina preparations.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Jianxian
authors.initials: J
authors.lastname: Lin
authors.forename: Ningyan
authors.initials: N
authors.lastname: Bai
authors.forename: Shibo
authors.initials: S
authors.lastname: Tang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Guanguang
authors.initials: G
authors.lastname: Feng
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012171:Retinal Vessels / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1