Construction of a linkage map based on a Lathyrus sativus backcross population and preliminary investigation of QTLs associated with resistance to ascochyta blight.
A linkage map of the Lathyrus sativus genome was constructed using 92 backcross individuals derived from a cross between an accession resistant (ATC 80878) to ascochyta blight caused by Mycosphaerella pinodes and a susceptible accession (ATC 80407). A total of 64 markers were mapped on the backcross population, including 47 RAPD, seven sequence-tagged microsatellite site and 13 STS/CAPS markers. The map comprised nine linkage groups, covered a map distance of 803.1 cM, and the average spacing between markers was 15.8 cM. Quantitative trait loci (QTL) associated with ascochyta blight resistance were detected using single-point analysis and simple and composite interval mapping. The backcross population was evaluated for stem resistance in temperature-controlled growth room trials. One significant QTL, QTL1, was located on linkage group 1 and explained 12% of the phenotypic variation in the backcross population. A second suggestive QTL, QTL2, was detected on linkage group 2 and accounted for 9% of the trait variation. The L. sativus R-QTL regions detected may be targeted for future intergenus transfer of the trait into accessions of the closely related species Pisum sativum.
Skiba
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Department of Biotechnology and Environmental Biology, RMIT University, Plenty Road, Bundoora, VIC, 3083, Australia. beata.skiba@rmit.edu.au
Ford
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Pang
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D001203:Ascomycota; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D007113:Immunity, Innate / Q000235:genetics*; D027807:Lathyrus / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D040641:Quantitative Trait Loci; D019105:Random Amplified Polymorphic DNA Technique