FTIR microscopy detection of cells infected with viruses.
Fourier-transform infrared (FTIR) microscopy is considered a comprehensive and sensitive method for detection of molecular changes in cells. The advantage of FTIR microspectroscopy over conventional FTIR spectroscopy is that it facilitates inspection of restricted regions of a cell culture or a tissue. We have shown that it is possible to apply FTIR microscopy as a sensitive and effective assay for the detection of cells infected with various members of the herpes family of viruses and retroviruses. Detectable and significant spectral differences between normal and infected cells were evident at early stages of the infection. Impressive changes in several spectroscopic parameters were seen in infected compared with uninfected cells. It seems that the change in spectral behavior is specific to the infecting virus, because cells infected with herpesviruses showed different spectral changes compared with cells infected with retroviruses.
Erukhimovitch
Vitaly
V
Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Talyshinsky
Marina
M
Souprun
Yelena
Y
Huleihel
Mahmoud
M
D000818:Animals; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D018259:Herpesvirus 1, Human / Q000378:metabolism; D051379:Mice; D008853:Microscopy / Q000379:methods*; D041681:NIH 3T3 Cells; D009053:Sarcoma Viruses, Murine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014709:Vero Cells; D014777:Virus Diseases / Q000175:diagnosis*