Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells.
The cytotoxic mechanism of protein-bound polysaccharide isolated from Phellinus linteus (PL, Mesima) has been investigated. PL inhibited the proliferation and colony formation of SW480 human colon cancer cells. Flow cytometry analysis showed that PL increased the populations of both apoptotic sub-G1 and G2/M phase. The result obtained from TUNEL assay corroborated apoptosis which was shown in flow cytometry. Western blot analysis suggested that PL-induced apoptosis and growth inhibition were associated with decrease in Bcl-2, increase of the release of cytochrome c, and reduced expression of cyclin B1. These results suggest that PL has a direct antitumor effect through apoptosis and cell cycle blockade in certain cancer cells.
Li
Ge
G
Department of Surgery ,College of Medicine, Cancer Research Institute, Chungnam National University and Hospital, 640 Daesa-Dong, Joong-Ku, Daejon 301-721, South Korea.
Kim
Dong-Hee
DH
Kim
Tae-Dong
TD
Park
Byoung-Jeon
BJ
Park
Hae-Duck
HD
Park
Jong-Il
JI
Na
Min-Kyun
MK
Kim
Hwan-Chul
HC
Hong
Nam-Doo
ND
Lim
Kyu
K
Hwang
Byung-Doo
BD
Yoon
Wan-Hee
WH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001487:Basidiomycota; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D011134:Polysaccharides