Optical biopsy of liver fibrosis by use of multiphoton microscopy.
We demonstrate the application of multiphoton microscopy in diagnosing toxin- (CCl4-) induced liver fibrosis in mice. Although hepatocyte autofluorescence does not vary significantly, different degrees of necrosis and stellate cell proliferation at necrotic sites in livers with fibrosis (ex vivo) can be detected easily from multiphoton-induced autofluorescence images by use of 780-nm excitation. Our result suggests that multiphoton microscopy can be developed into an effective technique for the detection and diagnosis of liver fibrosis in vivo.
Lee
Hsuan-Shu
HS
Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
Liu
Yuan
Y
Chen
Hsiao-Ching
HC
Chiou
Ling-Ling
LL
Huang
Guan-Tarn
GT
Lo
Wen
W
Dong
Chen-Yuan
CY
D000818:Animals; D001706:Biopsy / Q000295:instrumentation / Q000379:methods; D002251:Carbon Tetrachloride; D022781:Hepatocytes / Q000473:pathology*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*
D002251:Carbon Tetrachloride