title: Identification of a novel nephropathogenic infectious bronchitis virus in Israel.
abstract: A novel infectious bronchitis variant, designated as IS/885/00, associated with nephritis, was isolated from outbreaks in 23 broiler farms in Israel. The virus was first identified by reverse transcriptase-polymerase chain reaction and showed a distinct restriction fragment length polymorphism pattern from previously described Israeli isolates. Sequence analysis of the S1 gene and the deduced amino acid sequence revealed 97.2% protein similarity to genotype IS/ 720/99 and 71.6% similarity to the vaccine strain H120, the only strain permitted for use in this country. A database search in GenBank revealed a closely related isolate from Egypt, Egypt/Beni-Seug/01, with 96.6% similarity. Other published nephropathogenic infectious bronchitis virus strains/isolates shared less than 77% similarity with IS/885/00. A vaccine protection test in specific-pathogen-free chicks indicated 91% protection to the trachea and only 25% protection to the kidneys in vaccinated birds challenged with IS/885/00.
authors.affiliation: Division of Avian and Aquatic Animal Diseases, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan, 50250, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Meir
authors.forename: E
authors.initials: E
authors.lastname: Rosenblut
authors.forename: S
authors.initials: S
authors.lastname: Perl
authors.forename: N
authors.initials: N
authors.lastname: Kass
authors.forename: G
authors.initials: G
authors.lastname: Ayali
authors.forename: S
authors.initials: S
authors.lastname: Perk
authors.forename: E
authors.initials: E
authors.lastname: Hemsani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002645:Chickens / Q000276:immunology / Q000821:virology*; D016000:Cluster Analysis; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000276:immunology*; D007557:Israel; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014132:Trachea / Q000821:virology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D014765:Viral Vaccines; C031728:sigma 1 protein, reovirus