Genes required for Drosophila nervous system development identified by RNA interference.
RNA interference was used to screen 3,314 Drosophila double-stranded RNAs, corresponding to approximately 25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the genes are unknown. Other genes encode protein kinases, transcription factors, and signaling proteins, as well as proteins with other functions.
Ivanov
Andrej I
AI
Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Rovescalli
Alessandra C
AC
Pozzi
Paola
P
Yoo
Siuk
S
Mozer
Brian
B
Li
Hsi-Ping
HP
Yu
Shu-Hua
SH
Higashida
Haruhiro
H
Guo
Vicky
V
Spencer
Michael
M
Nirenberg
Marshall
M
D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D009154:Mutation; D009420:Nervous System / Q000196:embryology*; D010641:Phenotype; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics
D012330:RNA, Double-Stranded