title: The Ca2+ channel alpha2delta-1 subunit determines Ca2+ current kinetics in skeletal muscle but not targeting of alpha1S or excitation-contraction coupling.
abstract: Auxiliary channel subunits regulate membrane expression and modulate current properties of voltage-activated Ca(2+) channels and thus are involved in numerous important cell functions, including muscle contraction. Whereas the importance of the alpha(1S), beta(1a), and gamma Ca(2+) channel subunits in skeletal muscle has been determined by using null-mutant mice, the role of the alpha(2)delta-1 subunit in skeletal muscle is still elusive. We addressed this question by small interfering RNA silencing of alpha(2)delta-1 in reconstituted dysgenic (alpha(1S)-null) myotubes and in BC3H1 skeletal muscle cells. Immunofluorescence labeling of the alpha(1S) and alpha(2)delta-1 subunits and whole cell patch clamp recordings demonstrated that triad targeting and functional expression of the skeletal muscle Ca(2+) channel were not compromised by the depletion of the alpha(2)delta-1 subunit. The amplitudes and voltage dependences of L-type Ca(2+) currents and of the depolarization-induced Ca(2+) transients were identical in control and in alpha(2)delta-1-depleted muscle cells. However, alpha(2)delta-1 depletion significantly accelerated the current kinetics, most likely by the conversion of slowly activating into fast activating Ca(2+) channels. Reverse transcription-PCR analysis indicated that alpha(2)delta-1 is the exclusive isoform expressed in differentiated BC3H1 cells and that depletion of alpha(2)delta-1 was not compensated by the up-regulation of any other alpha(2)delta isoform. Thus, in skeletal muscle the Ca(2+) channel alpha(2)delta-1 subunit functions as a major determinant of the characteristic slow L-type Ca(2+) current kinetics. However, this subunit is not essential for targeting of Ca(2+) channels or for their primary physiological role in activating skeletal muscle excitation-contraction coupling.
authors.affiliation: Department of Physiology and Medical Physics, Innsbruck Medical University, Austria.
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Obermair
authors.forename: Gerlinde
authors.initials: G
authors.lastname: Kugler
authors.forename: Sabine
authors.initials: S
authors.lastname: Baumgartner
authors.forename: Petronel
authors.initials: P
authors.lastname: Tuluc
authors.forename: Manfred
authors.initials: M
authors.lastname: Grabner
authors.forename: Bernhard E
authors.initials: BE
authors.lastname: Flucher
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010957:Plasmids; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D034741:RNA, Small Interfering