title: Detection of HCV antibodies in oral fluid.
abstract: Although conventionally the detection of HCV antibodies is carried out on serum, the collection of oral fluid is non-invasive, safe and cost effective. In this study, the efficacy of the detection of HCV antibodies in oral fluid was assessed. 73 anti-HCV positive and 73 anti-HCV negative paired serum/oral fluid samples, drawn from patients visiting a Belgian academic hospital, were tested using the modified Ortho HCV 3.0 and LIA confirmation assay. Performing the test on oral fluid with the modified protocol, 61/73 anti-HCV positive samples were tested positive, while 73/73 anti-HCV negative samples were tested negative, giving a sensitivity and specificity of 83.6% (95% CI: 72.7-90.9%) and 100.0% (95% CI: 93.8-100.0%), respectively. Comparing S/CO of concordantly positive and negative samples, the cut-off point was lowered by 30% resulting in a sensitivity of 89.0% (95% CI: 79.0-94.8%) while the specificity remained 100.0% (95% CI: 93.8-100.0%). The confirmation assay was carried out as described by the manufacturer, diluting the oral fluid 1:10. Testing paired samples gave a concordance of 85.6% (125/146), yielding no more accurate results. These findings suggested that the modified ELISA method for anti-HCV detection in oral fluid can be used for epidemiological surveys.
authors.affiliation: Department of Microbiology, Scientific Institute of Public Health, Section of Virology, Juliette Wytsmanstreet 14, B-1050 Brussels, Belgium. liesbet.decook@iph.fgov.be
authors.forename: L
authors.initials: L
authors.lastname: De Cock
authors.forename: V
authors.initials: V
authors.lastname: Hutse
authors.forename: E
authors.initials: E
authors.lastname: Verhaegen
authors.forename: S
authors.initials: S
authors.lastname: Quoilin
authors.forename: H
authors.initials: H
authors.lastname: Vandenberghe
authors.forename: R
authors.initials: R
authors.lastname: Vranckx
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D001800:Blood Specimen Collection / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000032:analysis*; D006801:Humans; D012463:Saliva / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D018937:Hepatitis C Antibodies