Harnessing phytochrome's glowing potential.
Directed evolution of a cyanobacterial phytochrome was undertaken to elucidate the structural basis of its light sensory activity by remodeling the chemical environment of its linear tetrapyrrole prosthetic group. In addition to identifying a small region of the apoprotein critical for maintaining phytochrome's native spectroscopic properties, our studies revealed a tyrosine-to-histidine mutation that transformed phytochrome into an intensely red fluorescent biliprotein. This tyrosine is conserved in all members of the phytochrome superfamily, implicating direct participation in the primary photoprocess of phytochromes. Fluorescent phytochrome mutants also hold great promise to expand the present repertoire of genetically encoded fluorescent proteins into the near infrared.
Fischer
Amanda J
AJ
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
Lagarias
J Clark
JC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003116:Color; D019143:Evolution, Molecular; D005453:Fluorescence; D007259:Infrared Rays; D015394:Molecular Structure; D005810:Multigene Family / Q000235:genetics; D009154:Mutation / Q000235:genetics; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D010798:Phycocyanin / Q000378:metabolism; D010834:Phytochrome / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism; D045725:Tetrapyrroles
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D011758:Pyrroles; D045725:Tetrapyrroles; D010798:Phycocyanin; D010834:Phytochrome; C011069:phycocyanobilin; D011494:Protein Kinases; C413623:Cph1 phytochrome protein, bacteria