title: Abnormal RNA processing and altered expression of serin-rich proteins in minimal-change nephrotic syndrome.
abstract: Mechanisms underlying the pathophysiology of minimal-change nephrotic syndrome (MCNS), the most frequent glomerular disease in children, remain elusive, but recent findings argue for a T cell dysfunction. Starting from a differential cDNA library from T cells of a patient under relapse and remission, we identified 16 transcripts specific for MCNS. All of these transcripts that were selectively up-regulated during the relapse phase of the disease were generated by alternative splicing of known genes. This abnormal RNA expression was associated with a down-regulation of serin-rich protein 75 and serin-rich protein 40, two proteins involved in mRNA splicing. Taken together, these data suggest that T cell dysfunction in MCNS is associated with abnormal mRNA splicing.
authors.affiliation: Unité INSERM U581, Hôpital Henri Mondor, 51 Avenue du Mal de Lattre Tassigny, 94010 Creteil, France. philippe.grimbert@umontreal.ca
authors.forename: Philippe
authors.initials: P
authors.lastname: Grimbert
authors.forename: Vincent
authors.initials: V
authors.lastname: Audard
authors.forename: Asta
authors.initials: A
authors.lastname: Valanciute
authors.forename: André
authors.initials: A
authors.lastname: Pawlak
authors.forename: Philippe
authors.initials: P
authors.lastname: Lang
authors.forename: Georges
authors.initials: G
authors.lastname: Guellaën
authors.forename: Djillali
authors.initials: D
authors.lastname: Sahali
mesh_terms: D017398:Alternative Splicing; D002648:Child; D015331:Cohort Studies; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D009404:Nephrotic Syndrome / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012313:RNA / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012008:Recurrence; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012694:Serine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012694:Serine; D012313:RNA