title: Effects of N-acetylcysteine on oxidant-antioxidant balance in oleic acid-induced lung injury.
abstract: The antioxidant and anti-inflammatory properties of N-acetylcysteine has been documented in many experimental lung injury models. Because intravenous injection of oleic acid induces histopathologic changes similar to those seen in human acute lung injury or acute respiratory distress syndrome, the authors evaluated the effects of N-acetylcysteine (NAC) on oxidative stress and lung damage in an oleic acid (OA)-induced lung injury model. Thirty-five rats were divided into 5 groups as sham, NAC, OA, pre-OA-NAC, and post-OA-NAC. Lung damage was induced by intravenous administration of oleic acid. Pre-OA-NACgroup received intravenous (IV) N-acetylcysteine 15 minutes before oleic acid infusion and post-OA-NAC group received IV N-acetylcysteine 2 hours after oleic acid infusion. In both of the N-acetylcysteine treatment groups, blood and tissue samples were collected 4 hours after oleic acid infusion, independent from the time of N-acetylcysteine infusion. In other groups, blood and tissue samples were collected 4 hours after ethanol, NAC, or OA infusions. Serum myeloperoxidase activity, total antioxidant capacity, malondialdehyde levels, and lung tissue Na+ - K+ ATPase activity were measured and light microscopic analyses of lung specimens were performed. The administration of N-acetylcysteine significantly restored Na+ - K+ ATPase activity and total antioxidant capacity levels and ameliorated lung architecture. N-acetylcysteine has been shown to have some attenuating effects in experimental animal studies. However, further investigations are necessary to suggest N-acetylcysteine as a treatment agent in critically ill patients with lung injury.
authors.affiliation: Department of Thoracic Surgery, University of Mersin School of Medicine, Mersin, Turkey. oguzkoksel@mersin.edu.tr
authors.forename: Oguz
authors.initials: O
authors.lastname: Koksel
authors.forename: Ali
authors.initials: A
authors.lastname: Ozdulger
authors.forename: Menderes
authors.initials: M
authors.lastname: Ercil
authors.forename: Lulufer
authors.initials: L
authors.lastname: Tamer
authors.forename: Bahadir
authors.initials: B
authors.lastname: Ercan
authors.forename: Ugur
authors.initials: U
authors.lastname: Atik
authors.forename: Leyla
authors.initials: L
authors.lastname: Cinel
authors.forename: Ismail
authors.initials: I
authors.lastname: Cinel
authors.forename: Arzu
authors.initials: A
authors.lastname: Kanik
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005260:Female; D007275:Injections, Intravenous; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D019301:Oleic Acid / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D019301:Oleic Acid; D008315:Malondialdehyde; D009195:Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase; D000111:Acetylcysteine