title: Detection of Cryptosporidium oocysts and Giardia cysts in swimming pool filter backwash water concentrates by flocculation and immunomagnetic separation.
abstract: The purpose of the present study was to evaluate techniques for detection of Giardia cysts and Cryptosporidium oocysts in swimming pool filter backwash water. Calcium carbonate flocculation was used for water samples concentration of 1 l filter backwash water samples. Immunomagnetic separation (IMS) was used for separation of cysts and oocysts from detrital material. Cysts and oocysts were then detected using direct immunofluorescence. ColorSeed C&G was used as an internal standard. Recoveries were examined at several processing points. Highest recoveries (23% Cryptosporidium, 18% Giardia) were obtained using lower filter backwash volumes, greater IMS volumes, and addition of Tween20 to backwash samples prior to processing. The combination of CaCO3 flocculation, IMS and direct immunofluorescence was found to be an effective tool for the detection and quantification of Giardia spp. and Cryptosporidium spp. in filter backwash water samples.
authors.affiliation: Departamento de Microbiologia e Parasitologia MIP, CCB, LaboratOrio de Virologia Aplicada Universidade Federal de Santa Catarina Brazil.
authors.forename: Juliane
authors.initials: J
authors.lastname: Greinert
authors.forename: Danielle
authors.initials: D
authors.lastname: Furtado
authors.forename: James
authors.initials: J
authors.lastname: Smith
authors.forename: Célia
authors.initials: C
authors.lastname: Monte Barardi
authors.forename: Cláudia
authors.initials: C
authors.lastname: Simões
mesh_terms: D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry; D003458:Cryptosporidium / Q000302:isolation & purification*; D005374:Filtration; D005427:Flocculation; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000302:isolation & purification*; D018189:Immunomagnetic Separation; D033661:Oocysts / Q000302:isolation & purification; D013551:Swimming Pools
chemical_list: D002119:Calcium Carbonate