Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin.
The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA.
Jing
Wang
W
Department of Pathophysiology, Hebei Medical University, Shijiazhuang, China.
Shuhan
Sun
S
Zhenlin
Hu
H
Fengjuan
Zhou
Z
Yiling
Ling
L
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004269:DNA, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005612:Freeze Drying; D016680:Genome, Bacterial; D009163:Mycobacterium bovis / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D004269:DNA, Bacterial