title: Temporal patterns of the embryonic intermediate filaments nestin and vimentin expression in the cerebral cortex of adult rats after cryoinjury.
abstract: The expression of two embryonic intermediate filaments, nestin and vimentin, in the rat brain at days 0 (control), 1, 4, 7 and 14 post-cryoinjury was studied to elucidate their roles in brain injury. Western blot analysis showed that both nestin and vimentin expressions in the ipsilateral cerebral cortex were significantly increased at 4 and 7 days post-cryoinjury, and were decreased at day 14 after cryoinjury. Immunohistochemistry showed that there were few nestin- and vimentin-positive cells in the cerebral cortex in normal controls. On days 4 and 7 post-injury, abundant glial cells in the periphery of the lesion were immunostained for nestin and/or vimentin; only vimentin was detected in the majority of inflammatory cells in the core lesion. These findings suggest that nestin and vimentin contribute to the repair of brain injury through the migration of activated cells and the formation of a glial scar.
authors.affiliation: Department of Veterinary Medicine, Cheju National University, Jeju 690-756, South Korea.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Seungjoon
authors.initials: S
authors.lastname: Kim
authors.forename: Jae-Kwang
authors.initials: JK
authors.lastname: Jin
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Sim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001930:Brain Injuries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D005615:Freezing; D007839:Functional Laterality / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014746:Vimentin / Q000378:metabolism*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014746:Vimentin