title: Identification and characterization of linear B-cell epitopes of beta-globulin, a major allergen of sesame seeds.
abstract: BACKGROUND
  The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
  
  
  OBJECTIVE
  This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd beta-globulin, the major allergen of sesame seed.
  
  
  METHODS
  A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on beta-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame beta-globulin served to identify the epitopes by using the dot-blot test.
  
  
  RESULTS
  At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the beta-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of beta-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
  
  
  CONCLUSIONS
  Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.
authors.affiliation: Department of Biotechnology and Food Engineering, Technion-Institute of Technology, Haifa 32000, Israel.
authors.forename: Neomi
authors.initials: N
authors.lastname: Wolff
authors.forename: Shmuel
authors.initials: S
authors.lastname: Yannai
authors.forename: Nathan
authors.initials: N
authors.lastname: Karin
authors.forename: Yael
authors.initials: Y
authors.lastname: Levy
authors.forename: Ram
authors.initials: R
authors.lastname: Reifen
authors.forename: Ilan
authors.initials: I
authors.lastname: Dalal
authors.forename: Uri
authors.initials: U
authors.lastname: Cogan
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D001609:Beta-Globulins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D018985:Epitopes, B-Lymphocyte; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007223:Infant; D008969:Molecular Sequence Data; D012639:Seeds / Q000276:immunology*; D031685:Sesamum / Q000276:immunology*
chemical_list: D000485:Allergens; D001609:Beta-Globulins; D018985:Epitopes, B-Lymphocyte; D007073:Immunoglobulin E