[The dynamic change of total alkaloids content from Pinellia ternate].
OBJECTIVE
To determine the alkaloids content of Pinellia ternate in different growing time for finding out the best harvest time.


METHODS
The alkaloid was extracted by chloroform from Pinellia ternate and tested at 417 nm with the spectroscopy by acid dye. The result was analyzed in One-Way ANOVA.


RESULTS
The calibration curve of alkaloid was linear( r = 0.9989). The recovery rate was perfect (100.1%). The alkaloids content in different growing time had very significant difference ( F = 12.789, P < 0.01).


CONCLUSIONS
This method is simple and quick. The alkaloids content in whole seedling stage is higher than that in other growing stages, while the mean total alkaloids output per plant is the highest in intensive seedling-downfallen stage which is considered as the best harvest time.
Zeng
Jianhong
J
Department of Biology, West China Normal University, Nanchong 637000.
Peng
Zhengsong
Z
Song
Jingyuan
J
Ma
Xiaojun
X
Wei
Shuhong
S
Luo
Yongcheng
Y
D000470:Alkaloids / Q000032:analysis*; D000704:Analysis of Variance; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D030021:Pinellia / Q000737:chemistry* / Q000254:growth & development; D035281:Plant Tubers / Q000737:chemistry / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013997:Time Factors
D000470:Alkaloids; D004396:Coloring Agents