title: Immunological properties of FMDV-gP64 fusion proteins expressed on SF9 cell and baculovirus surfaces.
abstract: In the present report, we characterized the immune response and the protection conferred by recombinant baculoviruses or infected insect cells expressing the fusions gp64-P1 and gp64-site A FMDV antigens. Mice, vaccinated intraperitoneally with gp64-P1 immunogens, showed a low-antibody response and a variable degree of protection. However, when mice received recombinant baculoviruses or infected insect cells expressing the fusion protein gp64-site A, high-ELISA and seroneutralizing titers (SNT) against FMDV were elicited. All mice immunized with Sf9 cells expressing FMDV site A developed a protective immune response against challenge with virulent FMDV, indicating that the baculovirus display of foreign epitopes is a promising approach to biosynthetic vaccines.
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Castelar, CC25 (1712), Buenos Aires, Argentina.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Tami
authors.forename: Andrea
authors.initials: A
authors.lastname: Peralta
authors.forename: Rosario
authors.initials: R
authors.lastname: Barbieri
authors.forename: Analía
authors.initials: A
authors.lastname: Berinstein
authors.forename: Elisa
authors.initials: E
authors.lastname: Carrillo
authors.forename: Oscar
authors.initials: O
authors.lastname: Taboga
mesh_terms: D000086042:3C Viral Proteases; D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D018411:Spodoptera / Q000166:cytology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases