title: Effect of anaerobic fungi on in vitro feed digestion by mixed rumen microflora of buffalo.
abstract: Five strains of anaerobic fungi isolated from the faeces of wild (hog deer, Cervus porcinus; blackbuck, Antelope cervicapra; spotted deer, Axis axis; nilgai, Baselophus tragocamelus) and rumen liquor of domestic (sheep, Ovies aries) ruminants showing high fibrolytic enzyme producing ability were added to mixed rumen microflora of buffalo to study their effect on the digestibility of lignocellulosic feed (wheat straw and wheat bran in the ratio of 80:20), enzyme production and fermentation end products in in vitro conditions. Among the 5 isolates studied, FNG5 (isolated from nilgai) showed the highest stimulating effect on apparent digestibility (35.31 +/- 1.61% vs. 28.61 +/- 1.55%; P < 0.05), true digestibility (43.64 +/- 1.73% vs. 35.37 +/- 1.65%; P < 0.01), neutral detergent fiber digestibility (29.30 +/- 2.58% vs. 18.47 +/- 2.12; P < 0.01) of feed 24 h after inoculation compared to the control group. The production of carboxymethyl cellulase, xylanase, acetyl esterase and beta-glucosidase was significantly (P < 0.05) higher in the FNG5 inoculated incubation medium. There was no improvement in the digestibility and enzyme production on the addition of the other 4 isolates. Total volatile fatty acid levels as well as the concentration of acetate, propionate, isobutyrate and valerate were significantly higher in the FNG5 added group as compared to the control group. The fungal isolate FNG5 from nilgai, a wild ruminant, was found to be superior to the other isolates tested and appears to have a potential to be used as a feed additive for improving fiber degradation in domestic ruminants.
authors.affiliation: Central Institute for Research on Buffaloes, Nabha 147201, Patiala, India.
authors.forename: Shyam Sunder
authors.initials: SS
authors.lastname: Paul
authors.forename: Devki Nandan
authors.initials: DN
authors.lastname: Kamra
authors.forename: Vadali Rama Bhadra
authors.initials: VR
authors.lastname: Sastry
authors.forename: Narottam Prasad
authors.initials: NP
authors.lastname: Sahu
authors.forename: Neeta
authors.initials: N
authors.lastname: Agarwal
mesh_terms: D000821:Animal Feed; D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D002020:Buffaloes / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism; D004063:Digestion; D004950:Esterases / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D066298:In Vitro Techniques; D008031:Lignin / Q000378:metabolism*; D020089:Piromyces / Q000201:enzymology* / Q000302:isolation & purification; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*; D014995:Xylosidases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D004950:Esterases; D014995:Xylosidases; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase