title: Association of CyclinD1 copy number changes with histological type in ovarian tumors.
abstract: Literature data on the occurrence of CCND1 alterations in ovarian tumors are insufficient. The objective of this study was to assess the incidence of CCND1 copy number changes in a large number of ovarian tumors and its relation to the tumor phenotype: degree of malignancy, histological type, tumor stage, and grade. Fluorescence in situ hybridization (FISH) for analysis of CCND1 copy number changes was applied on a collection of 1 006 ovarian tumors--468 malignant, 48 with low malignant potency, and 490 benign tumors--arranged in tissue microarray. CCND1 amplification was found in 8.46% of the malignant cases and in 8.11% of those with low malignant potency. It was not found in benign ovarian tumors. CCND1 amplification was associated with the mucinous type of ovarian cancer (p<0.0001). CCND1 genetic gain was revealed in 9.06% of the malignant tumors, in 2.70% of the tumors with low malignant potency, and in 4.87% of the benign ovarian tumors. CCND1 gains and amplifications were not associated with the tumor grade and stage. Our results suggest that CCND1 gains are early events in ovarian tumorogenesis.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia, Bulgaria.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Dimova
authors.forename: Boriana
authors.initials: B
authors.lastname: Zaharieva
authors.forename: Sashka
authors.initials: S
authors.lastname: Raicheva
authors.forename: Ivan
authors.initials: I
authors.lastname: Milev
authors.forename: Rumen
authors.initials: R
authors.lastname: Dimitrov
authors.forename: Nicolai
authors.initials: N
authors.lastname: Doganov
authors.forename: Marin
authors.initials: M
authors.lastname: Aleksandrov
authors.forename: Todor
authors.initials: T
authors.lastname: Todorov
authors.forename: Draga
authors.initials: D
authors.lastname: Toncheva
mesh_terms: D005260:Female; D018628:Gene Dosage; D019940:Genes, bcl-1 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D012189:Retrospective Studies