Physical and chemical stability of different formulations with superoxide dismutase.
Topical formulations with superoxide dismutase (SOD), a scavenger of superoxide radicals, have proved to be effective against some skin diseases. Nevertheless, formulations with proteins are susceptible to both chemical and physical instability. Three different formulations (anionic and non-ionic gel and emulsion) were developed and supplemented with SOD in order to determine the most stable formulation that would maintain SOD activity. Physical stability was evaluated by assessing the rheological behavior of the formulations stored at room temperature, 37 and 45 degrees C. Chemical stability was evaluated by the measurement of enzymatic activity in the formulations stored at room temperature and at 45 degrees C. Formulations showed a flow index less than one, characterizing pseudoplastic behavior. There was no significant difference in initial values of flow index, tixotropy or minimum apparent viscosity. Neither gel showed significant changes in minimum apparent viscosity concerning storage time or temperature, as well, SOD presence and its activity. The emulsion showed decreased viscosity by the 28th day, but no significant changes concerning storage temperature or SOD presence, although it showed a decreased activity. The addition of SOD to the formulations studied did not affect their physical stability but gel formulations seem to be better bases for enzyme addition.
Di Mambro
V M
VM
Department of Pharmaceutical Science, Faculty of Pharmaceutical Science, University of São Paulo, Brazil.
Campos
P M B G Maia
PM
Fonseca
M J V
MJ
D002626:Chemistry, Pharmaceutical; D004355:Drug Stability; D004655:Emulsions; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D005782:Gels; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry; D012212:Rheology; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D014783:Viscosity
D004655:Emulsions; D016166:Free Radical Scavengers; D005782:Gels; D011506:Proteins; D013482:Superoxide Dismutase