Estrogen and inflammation increase the excitability of rat temporomandibular joint afferent neurons.
Several painful conditions, including temporomandibular disorders (TMD), are more prevalent and more severe in women than in men. Although the physiological basis for this sex difference remains to be determined, it is likely that estrogen is an underlying factor. The present study was performed to test the hypotheses that estrogen increases the excitability of rat temporomandibular joint (TMJ) afferents and exacerbates the inflammation-induced sensitization of these sensory neurons. Retrogradely labeled TMJ neurons from ovariectomized rats and ovariectomized rats receiving chronic estrogen replacement were studied using whole cell patch-clamp techniques three days after injecting the TMJ with either saline or Complete Freund's Adjuvant to induce inflammation. Excitability was assessed with depolarizing current injection to determine action potential threshold, rheobase, and the response to suprathreshold stimuli. Spontaneous activity was also assessed. Both inflammation and estrogen increased the excitability of TMJ neurons as reflected by decreases in action potential threshold and rheobase and increases in the incidence of spontaneous activity. The effects were additive with neurons from rats receiving both estrogen and inflammation being the most excitable. The increases in excitability were associated with changes in passive properties and action potential waveform, suggesting that estrogen and inflammation affect the expression and/or properties of ion channels in TMJ neurons. Importantly, the influence of estrogen on both baseline and inflammation-induced changes in TMJ neuronal excitability may help explain the profound sex difference observed in TMD as well as suggest a novel target for the treatment of this pain condition.
Flake
Natasha M
NM
Department of Biomedical Sciences, Dental School, University of Maryland, 666 W. Baltimore St., Rm. 5-A-12, Baltimore, MD 21201, USA.
Bonebreak
David B
DB
Gold
Michael S
MS
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000704:Analysis of Variance; D000818:Animals; D002211:Capsaicin / Q000378:metabolism / Q000494:pharmacology; D002232:Carbocyanines / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D048429:Cell Size; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D025802:Electric Capacitance; D004558:Electric Stimulation / Q000379:methods; D004967:Estrogens / Q000097:blood / Q000494:pharmacology*; D005260:Female; D005620:Freund's Adjuvant; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D037102:Lectins / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D016900:Neurofilament Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000145:classification / Q000187:drug effects* / Q000528:radiation effects; D010052:Ovariectomy / Q000379:methods; D018408:Patch-Clamp Techniques / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013704:Temporomandibular Joint / Q000166:cytology*; D012668:Trigeminal Ganglion / Q000166:cytology; D053675:Versicans
C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines; D004967:Estrogens; D006023:Glycoproteins; D037102:Lectins; D016900:Neurofilament Proteins; C508324:Vcan protein, rat; C051774:neurofilament protein H; D053675:Versicans; D005620:Freund's Adjuvant; D002211:Capsaicin