A direct quantitative analysis method for monitoring biogenic volatile organic compounds released from leaves of Pelargonium hortorum in situ.
A direct quantitative method is presented that is based upon the use of multiple headspace solid phase microextraction (HS-SPME) to monitor biogenic volatile organic compounds (BVOCs) released from a living leaf of Pelargonium hortorum in situ. Seventeen BVOCs were detected by GC-MS after a single SPME extraction using a CAR/DVB/PDMS fibre. An internal standard was employed to determine the absolute amounts of seven terpenoid compounds released from a P. hortorum leaf. The quantitative analysis was performed over two days, with extraction preformed for 20 min every 3 h. The amount of volatiles extracted varied with the time of day, with two maxima recorded at 14:00 (day 1) and 17:00 (day 2), corresponding to 236 and 277 ng of the seven terpenoids recorded, respectively. These results indicate that multiple HS-SPME in combination with an internal standard is a simple, quick, and quantitative technique for analysising BVOC emissions from a live plant sample.
Deng
Xiaojun
X
Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Shanghai 200032, China. xjdeng@iris.sipp.ac.cn
Peng
Jinyin
J
Luo
Bin
B
Wei
Ming
M
Hu
Wenli
W
Du
Jiawei
J
D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D009930:Organic Chemicals / Q000032:analysis*; D031316:Pelargonium / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012044:Regression Analysis; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014835:Volatilization
D009930:Organic Chemicals; D012997:Solvents