Use of SPE and LC/TIS/MS/MS for rapid detection and quantitation of ketamine and its metabolite, norketamine, in urine.
Ketamine (K) has become more and more popular for drug abuse in recent years. A lot of pre-treatment work such as extraction and derivatizing increase difficulties in the tests for ketamine in biological specimens. A rapid method to detect and quantitate ketamine and its metabolite norketamine in urine used deuterated dilution followed by solid phase extraction and liquid chromatography/TurboIonSpray/tandem mass spectrometry (LC/TIS/MS/MS) is described. Control recovery for both low and high concentrations can reach to 90%. Ten ketamine positive urines were examinated by this method. Concentrations ranged from 114 to 2925 ng/mL and from 453 to 9805 ng/mL for norketamine. The method was sensitive, specific, accurate and provided easy operation to detect and quantitate ketamine and its metabolites in urine.
Wang
Kaung-Chaun
KC
Identification Division, Criminal Investigation Bureau, No. 5, Lane 553, Sector 4, Chung Hsiao E. Road, Taipei, Taiwan, ROC. kaung@email.cib.gov.tw
Shih
Tuo-Shi
TS
Cheng
Sheaw-Guey
SG
D000778:Anesthetics, Dissociative / Q000737:chemistry / Q000652:urine*; D005554:Forensic Medicine / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007649:Ketamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine*; D015394:Molecular Structure; D015813:Substance Abuse Detection / Q000379:methods*
D000778:Anesthetics, Dissociative; D007649:Ketamine; C033419:norketamine