title: Low-level lifetime exposure to cadmium decreases skeletal mineralization and enhances bone loss in aged rats.
abstract: The effects of low-level lifetime exposure to cadmium (Cd) on the skeleton mineral status and the risk of bone loss in the elderly were studied in an experimental model of human environmental exposure in non-Cd-polluted areas. Young female Wistar rats were exposed to 1 mg Cd/l in drinking water for 24 months. Bone mineral content (BMC), density (BMD) and area of the lumbar spine (L1-L5) and femur, and total skeleton BMD (T-BMD) were measured densitometrically at the baseline and after 6, 12, 18, and 24 months. Prevalence of osteopenia and osteoporosis was evaluated based on the BMD T score and Z score. Osteocalcin (OC) in the serum and total alkaline phosphatase (total ALP) in the serum, cortical and trabecular bone samples as bone formation markers, and C-terminal cross-linking telopeptide of type I collagen (CTX) in the serum and urine as bone resorption markers were measured. Calcium (Ca) and Cd concentrations in the serum/blood and urine were determined as well. In the Cd-exposed females, the L1-L5 and femur BMC and BMD at all the studied time points were lower compared to control. The exposure to Cd resulted in lower accumulation of peak bone mass, accelerated osteopenia, and enhanced the prevalence of osteoporosis in aged rats. The effect of Cd was more pronounced at the L1-L5 than at the femur. CTX concentration in the urine was decreased after 6 months and next increased compared to control, whereas the urinary loss of Ca was enhanced during the exposure to Cd. After 24 months of the treatment, the serum total ALP activity and the activity of this enzyme in cortical and trabecular bone decreased and serum CTX concentration increased, whereas the concentrations of OC and Ca were unchanged. The study clearly revealed that low-level lifetime exposure to Cd diminishes the accumulation of bone mass during skeletal growth and influences bone metabolism at maturity causing osteopenia, and enhances the age-related bone loss due to high turnover rate leading in consequence to osteoporosis in aged rats. The results together with our previous findings confirm the hypothesis that environmental exposure to Cd may be a risk factor for skeletal diseases.
authors.affiliation: Department of Toxicology, Medical University of Białystok, 15-222 Białystok, Poland. mmbr@poczta.onet.pl
authors.forename: M M
authors.initials: MM
authors.lastname: Brzóska
authors.forename: J
authors.initials: J
authors.lastname: Moniuszko-Jakoniuk
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000139:chemically induced / Q000453:epidemiology / Q000473:pathology; D016723:Bone Remodeling / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002104:Cadmium / Q000032:analysis / Q000494:pharmacology / Q000633:toxicity*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000097:blood / Q000652:urine; D003094:Collagen / Q000097:blood / Q000652:urine; D024042:Collagen Type I; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005269:Femur / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D008159:Lumbar Vertebrae / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D010024:Osteoporosis / Q000139:chemically induced* / Q000473:pathology; D010455:Peptides / Q000097:blood / Q000652:urine; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D002104:Cadmium; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium