The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis.
A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expression of fad104 was detected in adipocyte differentiable 3T3-L1 cells but not observed in the non-adipogenic cell line NIH-3T3. Moreover, the ability of 3T3-L1 cells to differentiate declined with the knockdown of fad104 by RNA interference, strongly indicating that fad104 functions as a positive regulator of adipogenesis.
Tominaga
Kei
K
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
Kondo
Chiharu
C
Johmura
Yoshikazu
Y
Nishizuka
Makoto
M
Imagawa
Masayoshi
M
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D018076:DNA, Complementary; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D005353:Fibronectins