Coupling of nanoflow liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry: real-time liquid chromatography run mapping on a MALDI plate.
The major obstacle in the use of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) instruments in the analysis of complex proteome samples is the lack of a direct coupling of a highly resolving separation technique with the mass spectrometer itself. To overcome this drawback, a spotting device for capillary and nanoflow liquid chromatography (LC) with a special liquid deposition principle for lowest volumes was developed. The instrument is able to perform MALDI spotting in real time in order to deposit the LC run on the MALDI plate, and therefore couples the high resolution power of nano-RP-HPLC separation directly with MALDI-MS. This work describes the development and optimization of a method for spotting with online matrix addition, and illustrates its use in the analysis of a complex proteome sample.
Nägele
Edgar
E
Agilent Technologies R&D and Marketing GmbH & Co. KG, Hewlett-Packard-Str. 8, 76337 Waldbronn, Germany. edgar_naegele@agilent.com
Vollmer
Martin
M
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008214:Lymphocytes / Q000737:chemistry; D036103:Nanotechnology; D010455:Peptides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*
D010455:Peptides