title: Potentiometric immunosensor using artificial antibody based on molecularly imprinted polymers.
abstract: A potentiometric artificial immunosensor based on a molecularly imprinted polymer was prepared as a detecting element in micro total analysis systems with the intent of providing easy clinical analysis. As the structure and transducing mechanism of this sensor are very simple, construction of a single microsensor should be quite easy. Multimicrosensor arrays applicable to several kinds of analytes will be attainable by both changing the template molecule to be imprinted and reducing the sensor size. The response characteristics of this sensor were evaluated by measuring the response potential to serotonin, which was used as a model material. The obtained sensor was highly responsive to serotonin in water but not to tryptamine, acetaminophen, or procainamide. This phenomenon confirms that the sensor recognizes serotonin and that it functions as a specific artificial immunosensor. Quick measurement is possible because the response time, defined as the time required to achieve 95% of the magnitude of the equilibrated signal, correspond to approximately 12 s. The sensor's determination and detection limits were found to be 1 mumol/L and 100 pmol/L, respectively. These results suggest that our strategy can be applied to construction of a potentiometric artificial immunosensor.
authors.affiliation: Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan. kitade@mb.kyoto-phu.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kitade
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kitamura
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Konishi
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Takegami
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Okuno
authors.forename: Michie
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Manabu
authors.initials: M
authors.lastname: Wakabayashi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Muramatsu
mesh_terms: D000082:Acetaminophen / Q000032:analysis; D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D002138:Calibration; D011108:Polymers / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation*; D011342:Procainamide / Q000032:analysis; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis; D014363:Tryptamines / Q000032:analysis
chemical_list: D000906:Antibodies; D011108:Polymers; D014363:Tryptamines; D012701:Serotonin; D000082:Acetaminophen; C030820:tryptamine; D011342:Procainamide