Bovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5.
Bovine herpesvirus 1 glycoprotein D (gD) gene expression by recombinant replication defective human adenovirus type 5 (HAdV-5) was investigated in calves using indirect immunofluorescence microscopy (IIFM), confocal laser scanning microscopy (CLSM) and RT-PCR. One fold intranasal instillation of HAdV-5-expressing gD in the cattle upper respiratory tract showed a short term expression of at least 5 days, but not 10 days, limited only to epithelial cells localised in the epithelium of the nasal mucosa in one out of six calves. Observed limited gene transfer into well differentiated cattle airway epithelial cells must be taken into consideration in order to enhance transfection efficiency, and consequently the vaccine potential of this vector.
Gogev
Sacha
S
Virology, Department of Infectious and Parasitic diseases, Faculty of Veterinary Medicine, University of Liège, boulevard de Colonster 20, B43b, 4000 Liège, Belgium.
Georgin
Jean-Pierre
JP
Schynts
Frédéric
F
Vanderplasschen
Alain
A
Thiry
Etienne
E
D000260:Adenoviruses, Human; D000281:Administration, Intranasal; D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000276:immunology; D005822:Genetic Vectors; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology; D012137:Respiratory System / Q000276:immunology*; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis*
D022283:Herpesvirus Vaccines; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins