title: Differential expression of protein kinase C alpha and delta in testes of mouse at various stages of development.
abstract: Protein kinase C (PKC) describes a family of serine/threonine protein kinases, and multiple isoforms are expressed in various mammalian tissues. In the present study, we examined the expression of PKC-alpha and PKC-delta at protein and mRNA level in mouse testis by Western blotting and RT-PCR. We also examined the expression of both PKC isoenzymes in the developing mouse testis. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC-alpha were uniformly distributed; but PKC-delta expression occurred in the testes of 3-week-old mice, perhaps even at a relatively late stage in spermatid development. The results suggest that each isoenzyme may have different functional roles in processing and modulating physiological cellular responses of spermatogenesis.
authors.affiliation: Department of Biochemistry, China Medical University, Shenyang, Liaoning Province, PRC.
authors.forename: Chen
authors.initials: C
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Vénuste
authors.initials: V
authors.lastname: Gasana
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: ZhiHong
authors.initials: Z
authors.lastname: Zong
authors.forename: BingZhi
authors.initials: B
authors.lastname: Yu
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism*; D051745:Protein Kinase C-delta / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development*
chemical_list: D007527:Isoenzymes; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta