title: Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core.
abstract: Pollen from cedar and cypress trees is a major cause of seasonal hypersensitivity in humans in several regions of the Northern Hemisphere. We report the first crystal structure of a cedar allergen, Jun a 1, from the pollen of the mountain cedar Juniperus ashei (Cupressaceae). The core of the structure consists primarily of a parallel beta-helix, which is nearly identical to that found in the pectin/pectate lyases from several plant pathogenic microorganisms. Four IgE epitopes mapped to the surface of the protein are accessible to the solvent. The conserved vWiDH sequence is covered by the first 30 residues of the N terminus. The potential reactive arginine, analogous to the pectin/pectate lyase reaction site, is accessible to the solvent, but the substrate binding groove is blocked by a histidine-aspartate salt bridge, a glutamine, and an alpha-helix, all of which are unique to Jun a 1. These observations suggest that steric hindrance in Jun a 1 precludes enzyme activity. The overall results suggest that it is the structure of Jun a 1 that makes it a potent allergen.
authors.affiliation: Sealy Center for Structural Biology, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0647, USA. edcz@xray.utmb.edu
authors.forename: Edmund W
authors.initials: EW
authors.lastname: Czerwinski
authors.forename: Terumi
authors.initials: T
authors.lastname: Midoro-Horiuti
authors.forename: Mark A
authors.initials: MA
authors.lastname: White
authors.forename: Edward G
authors.initials: EG
authors.lastname: Brooks
authors.forename: Randall M
authors.initials: RM
authors.lastname: Goldblum
mesh_terms: D000485:Allergens / Q000737:chemistry*; D052179:Antigens, Plant; D003461:Crystallography; D000939:Epitopes / Q000737:chemistry; D018653:Juniperus / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D010940:Plant Proteins; C121951:jun a 1 protein, Juniperus ashei