title: RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein.
abstract: Differential screening of a cDNA library constructed using poly(A)(+) RNA from suspension-cultured rice cells treated with jasmonic acid (JA) for 1/2h yielded a cDNA of a gene tentatively named RERJ1 that is upregulated in response to exogenous JA. Northern blot analysis indicated that the RERJ1 mRNA levels peaked at 1/2-1h after the addition of jasmonic acid and then decreased gradually. RERJ1 encodes a transcriptional regulator with a basic helix-loop-helix motif. The phenotypes of transgenic rice plants overexpressing sense or antisense RERJ1 mRNA demonstrated that RERJ1 is involved in the growth inhibition of rice shoots caused by JA. Other biological functions of RERJ1 are discussed from an evolutionary standpoint.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kiribuchi
authors.forename: Miho
authors.initials: M
authors.lastname: Sugimori
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Takeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Otani
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.forename: Haruko
authors.initials: H
authors.lastname: Onodera
authors.forename: Masashi
authors.initials: M
authors.lastname: Ugaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tomiyama-Akimoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Minami
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
mesh_terms: D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D018257:Helix-Loop-Helix Motifs / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000502:physiology*; D010940:Plant Proteins; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C495143:RERJ1 protein, rice