Depolymerization of beta-chitin to mono- and disaccharides by the serum fraction from the para rubber tree, Hevea brasiliensis.
The serum fraction of latex from Hevea brasiliensis, the para rubber tree, is known to contain an endo-chitinolytic enzyme, hevamine. Herein the activity of the rubber serum towards beta-chitin is investigated. The serum contained 6 mg/mL of protein and a chitinolytic activity of 18 mU permg of protein. The optimum ratio of enzyme to chitin was 0.22 mU/mg, and the optimum substrate concentration was 60 mg/mL. The optimum pH range was pH2-4, and the optimum temperature was 45 degrees C. At these conditions both (GlcNAc)2 and GlcNAc were produced in a molar ratio of approximately 2:1. The hydrolysis of 300 mg of chitin with 64 mU of the rubber serum for 8 days under the optimum conditions gave 39 mg of GlcNAc and 108 mg of (GlcNAc)2 as determined by HPLC. Mixing the rubber serum preparation with an Aspergillus niger pectinase preparation containing beta-N-acetylhexosaminidase can be used to produce almost exclusively the GlcNAc monomer in about 50% yield.
Klaikherd
Akamol
A
Department of Chemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
Siripastr Jayanta
M L
ML
Boonjawat
Jariya
J
Aiba
Sei-ichi
S
Sukwattanasinitt
Mongkol
M
D001234:Aspergillus niger / Q000201:enzymology; D002686:Chitin / Q000737:chemistry*; D004187:Disaccharides / Q000138:chemical synthesis*; D028482:Hevea / Q000737:chemistry* / Q000201:enzymology; D008958:Models, Molecular; D009005:Monosaccharides / Q000138:chemical synthesis*; D010936:Plant Extracts / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry; D013997:Time Factors
D004187:Disaccharides; D009005:Monosaccharides; D010936:Plant Extracts; D002686:Chitin; D011096:Polygalacturonase