Application of the 19F NMR technique to observe binding of the general anesthetic halothane to human serum albumin.
19F NMR techniques were employed to characterize the binding property of the widely used general anesthetic halothane with human serum albumin (HSA). It was found that 19F(1H) NOE and 2D 1H-19F HOESY experiments detected intermolecular NOEs between halothane 19F and HSA protons. Measurements of the diffusion coefficients for halothane were also carried out by 1H and 19F NMR, indicating the interaction of halothane with HSA. The present results indicate that these techniques are very suitable to identify a fluorine-containing ligand binding with a protein receptor in the drug-discovery process.
Shikii
Kazuaki
K
Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba 263-8522, Japan.
Sakurai
Satoshi
S
Utsumi
Hiroaki
H
Seki
Hiroko
H
Tashiro
Mitsuru
M
D018685:Anesthetics, Inhalation / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D006221:Halothane / Q000737:chemistry*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*
D018685:Anesthetics, Inhalation; D012709:Serum Albumin; D005461:Fluorine; D006221:Halothane