[Regulation of pre-mRNA alternative spicing in U251 cells by TPA].
By using the mini-gene construct containing partial sequence of Bcl-X gene as model, we examined the function of TPA on Bcl-X pre-mRNA alternative splicing in vivo and vitro with RT-PCR and site-directed mutagesis assay. The results show that PKA signaling system can regulate Bcl-X pre-mRNA alternative splicing, the possible mechanism is that the responsible sequence affect the choice of the 5'-downstream or upstream splice site of Bcl-X pre-mRNA.
Li
Chang-You
CY
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.
Chu
Jia-You
JY
Liu
Xiao-Juan
XJ
Yu
Jian-Kun
JK
Huang
Xiao-Qin
XQ
ShiI
Li
L
Zhu
Yong-Zhang
YZ
Na
Jian-Bo
JB
Xie
Jiu-Yong
JY
D017398:Alternative Splicing; D000818:Animals; D005419:Flavonoids / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; D012322:RNA Precursors; D051020:bcl-X Protein; C061097:breviscapine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate