Progress and challenges in profiling the dynamics of chromatin and transcription factor binding with DNA microarrays.
ChIP-chip, or chromatin immunoprecipitation followed by DNA microarray analysis, has proven to be an efficient means of mapping protein-genome interactions. Recent experiments using this tool are beginning to reveal the complex dynamics of transcription factor binding and chromatin organization, and how these processes interact with each other to generate a cellular response to environmental and developmental cues. Data derived from this approach, particularly data involving chromatin components and histone modifications, might be affected by assumptions underlying the procedure, and the data might be made more useful by adoption of standardized whole-genome microarray platforms.
Hanlon
Sean E
SE
Department of Biology and Carolina Center for the Genome Sciences, CB #3280, 202 Fordham Hall, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
Lieb
Jason D
JD
D000818:Animals; D002843:Chromatin / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D016678:Genome; D006657:Histones / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*
D002843:Chromatin; D006657:Histones; D014157:Transcription Factors