Selection of single-chain antibodies against the VP8* subunit of rotavirus VP4 outer capsid protein and their expression in Lactobacillus casei.
Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.
Monedero
Vicente
V
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot, Spain.
Rodríguez-Díaz
Jesús
J
Viana
Rosa
R
Buesa
Javier
J
Pérez-Martínez
Gaspar
G
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology*; D002460:Cell Line; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D003470:Culture Media; D006801:Humans; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D012401:Rotavirus
D000914:Antibodies, Viral; D036022:Capsid Proteins; D022801:Complementarity Determining Regions; D003470:Culture Media; D019151:Peptide Library; C060339:VP4 protein, Rotavirus