Altered renal response to acute volume expansion in transgenic rats harboring the human tissue kallikrein gene.
The renal response to acute volume expansion was investigated in transgenic (TGR) rats harboring the human tissue kallikrein gene. After a primer injection of 0.9% NaCl (3 ml/100 g, i.v), Sprague-Dawley (SD) or TGR rats received a continuous infusion of 0.9% NaCl (15 microl/100 g/min, i.a.) through a catheter placed into the carotid artery. Acute volume expansion was produced by a second injection of 0.9% NaCl (2 ml/100 g, i.v.) 65 min after the first injection. Plasma vasopressin (AVP) and atrial natriuretic peptide (ANP) concentration was measured before and within 10 min of volume expansion. TGR animals presented a blunted response to acute volume expansion evidenced by an attenuated increase in total and fractional water and sodium excretion. Before or after volume expansion, plasma AVP and ANP did not differ between SD and TGR. Pre-treatment with the BK-B2 antagonist HOE-140 (7.5 microg/100 g. i.a) partially improved the renal response of TGRs and severely blunted the response in SD rats. These data show that TGR (hKLK1) rats have an impaired renal response to acute volume expansion that can not be accounted for by changes in the release of AVP or ANP.
Passaglio
Kátia T
KT
Laboratório de Hipertensão, Instituto de Ciências Biológicas and Laboratório de Endocrinologia, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, 31270-901, Belo Horizonte, Brazil.
Baltatu
Ovidiu
O
Machado
Raquel P
RP
dos Reis
Adelina M
AM
Pesquero
João B
JB
Bader
Michael
M
Santos
Robson A S
RA
D000818:Animals; D030801:Animals, Genetically Modified; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001920:Bradykinin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D020840:Tissue Kallikreins / Q000235:genetics / Q000378:metabolism*
C065679:icatibant; D009320:Atrial Natriuretic Factor; D020840:Tissue Kallikreins; D001920:Bradykinin