title: Determination of lycopene in food by on-line SFE coupled to HPLC using a single monolithic column for trapping and separation.
abstract: A method that would eliminate the degradation of lycopene during analysis was developed. Supercritical fluid extraction (SFE) with carbon dioxide as the extraction medium was connected on-line to high performance liquid chromatography (HPLC) where a single monolithic column was used for trapping and the subsequent separation of analytes. The method was linear over the studied range (0.1-2.5 microg), and it was repeatable (R.S.D. 3.9%), sensitive (LOD = 0.5 ng) and fast (35 min). Lycopene was determined in tomatoes, fruit and several food products. Because of the on-line construction, lycopene was not in contact with air or light during the whole procedure and the amount analysed should therefore correspond to the real amount in the sample.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, PO. Box 55, FIN-00014 Helsinki, Finland.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pól
authors.forename: Tuulia
authors.initials: T
authors.lastname: Hyötylänen
authors.forename: Outi
authors.initials: O
authors.lastname: Ranta-Aho
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Riekkola
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D000077276:Lycopene; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D002338:Carotenoids; D000077276:Lycopene