title: Optimization of medium by orthogonal matrix method for submerged mycelial culture and exopolysaccharide production in Collybia maculata.
abstract: Optimization of submerged culture conditions for the production of mycelial growth and exopolysaccharides (EPSs) by Collybia maculata was investigated. The optimum temperature and the initial pH for EPS production in a shake-flask culture of C. maculata were found to be 20 degrees C and 5.5, respectively. Among the various medium's constituents examined, glucose, Martone A-1, K(2)HPO(4), and CaCl(2) were the most suitable carbon, nitrogen, and mineral sources for EPS production, respectively. The optimum concentration of the medium's ingredients determined using the orthogonal matrix method was as follows: 30 g/L of glucose, 20 g/L of Martone A-1, 1 g/L of K(2)HPO(4), and 1 g/L of CaCl(2). Under the optimized culture conditions, the maximum concentration of EPSs in a 5-L stirred-tank reactor was 2.4 g/L, which was approximately five times higher than that in the basal medium. A comparative fermentation result showed that the EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor despite the lower mycelial growth rate. The specific productivities and the yield coefficients in the airlift reactor were higher than those in the stirred-tank reactor even though the volumetric productivities were higher in the stirred-tank reactor than in the airlift reactor.
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Lim
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Joo
authors.forename: Hyun Oh
authors.initials: HO
authors.lastname: Kim
authors.forename: Jang Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D000465:Algorithms; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005163:Factor Analysis, Statistical; D005656:Fungal Proteins / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D011134:Polysaccharides; D002244:Carbon