title: Characterization of murine gammaherpesvirus 68 glycoprotein B.
abstract: Murine gammaherpesvirus 68 (MHV-68) glycoprotein B (gB) was identified in purified virions by immunoblotting, immunoprecipitation, and immunoelectron microscopy. It was synthesized as a 120-kDa precursor in infected cells and cleaved into 65-kDa and 55-kDa disulfide-linked subunits close to the time of virion release. The N-linked glycans on the cleaved, virion gB remained partially endoglycosidase H sensitive. The processing of MHV-68 gB therefore appears similar to that of Kaposi's sarcoma-associated herpesvirus gB and human cytomegalovirus gB.
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.
authors.forename: Filipa B
authors.initials: FB
authors.lastname: Lopes
authors.forename: Susanna
authors.initials: S
authors.lastname: Colaco
authors.forename: Janet S
authors.initials: JS
authors.lastname: May
authors.forename: Philip G
authors.initials: PG
authors.lastname: Stevenson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018144:Rhadinovirus / Q000737:chemistry / Q000502:physiology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000502:physiology; D014771:Virion / Q000502:physiology
chemical_list: D004220:Disulfides; D014759:Viral Envelope Proteins; C089386:glycoprotein B, murine gammaherpesvirus 68