title: Effects of short and long incubations on DNA fragmentation of testicular sperm.
abstract: DNA fragmentation in testicular sperm from men with obstructive azoospermia is increased by 4-hour and 24-hour incubations and after cryopreservation with the effect is intensified by post-thaw incubation. Testicular sperm for use in intracytoplasmic sperm injection (ICSI) should be injected without delay.
authors.forename: Lauren H
authors.initials: LH
authors.lastname: Dalzell
authors.forename: Carmel M
authors.initials: CM
authors.lastname: McVicar
authors.forename: Neil
authors.initials: N
authors.lastname: McClure
authors.forename: Deborah
authors.initials: D
authors.lastname: Lutton
authors.forename: Sheena E M
authors.initials: SE
authors.lastname: Lewis
mesh_terms: D018929:Cell Culture Techniques; D015925:Cryopreservation; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D013094:Spermatozoa; D013737:Testis; D013997:Time Factors