Poly(3-guanidinopropyl methacrylate): a novel cationic polymer for gene delivery.
A cationic polymethacrylate with a guanidinium side group was designed in order to create a polymer with cell membrane-penetrating properties such as Tat or other arginine-rich peptides. The polymer, poly(3-guanidinopropyl methacrylate), abbreviated as pGuaMA, was synthesized by free radical polymerization. The DNA-condensing properties of pGuaMA (Mw 180 kDa) were investigated via dynamic light scattering and zeta potential measurements, and small, positively charged particles (110 nm, +37 mV) were found. It was shown that polyplexes based on pGuaMA were able to transfect COS-7 cells efficiently in the absence of serum, while under the same conditions poly(arginine) (pArg) polyplexes did not show detectable transfection levels. Addition of a membrane-disrupting peptide, INF 7, derived from the influenza virus, to preformed pGuaMA polyplexes did result in approximately 2 times increased transfection levels. DLS, zeta potential measurements, gel electrophoresis, and ethidium bromide displacement measurements indicated that serum induced aggregation of the polyplexes at high polymer/plasmid ratios, while at low polymer/plasmid ratios the polarity of the polyplexes reversed likely due to adsorption of negatively charged proteins on their surface. Likely, the unfavorable interactions of pGuaMA polyplexes with serum proteins is the reason for the absent transfection activity of these polyplexes in the presence of serum. Confocal laser scanning microscopy indicated cellular internalization via endocytosis of both polyplexes and free polymer. Thus, pGuaMA polyplexes enter cells, as reported for other polyplexes, by endocytosis and not, as hypothesized, via direct membrane passage.
Funhoff
Arjen M
AM
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, 3508 TB, Utrecht, The Netherlands.
van Nostrum
Cornelus F
CF
Lok
Martin C
MC
Fretz
Marjan M
MM
Crommelin
Daan J A
DJ
Hennink
Wim E
WE
D000818:Animals; D019556:COS Cells; D002412:Cations; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods; D018390:Gene Targeting / Q000379:methods*; D019791:Guanidine / Q000008:administration & dosage / Q000138:chemical synthesis*; D011108:Polymers / Q000008:administration & dosage / Q000138:chemical synthesis*; D011109:Polymethacrylic Acids / Q000008:administration & dosage / Q000138:chemical synthesis*; D014162:Transfection / Q000379:methods
D002412:Cations; D011108:Polymers; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D004247:DNA; D019791:Guanidine