title: Reactivity of anti-Nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental Nipah and Hendra virus infections.
abstract: The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for immunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tanimura
authors.forename: Tadao
authors.initials: T
authors.lastname: Imada
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kashiwazaki
authors.forename: Shahirudin
authors.initials: S
authors.lastname: Shamusudin
authors.forename: Sharifah
authors.initials: S
authors.lastname: Syed Hassan
authors.forename: Aziz
authors.initials: A
authors.lastname: Jamaluddin
authors.forename: Gail
authors.initials: G
authors.lastname: Russell
authors.forename: John
authors.initials: J
authors.lastname: White
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D045464:Henipavirus Infections / Q000175:diagnosis / Q000276:immunology*; D006736:Horses; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D045405:Nipah Virus / Q000276:immunology*; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral