GMP and AMP as methyl radical traps in the reaction with pentaamminemethylcobalt(III).
C8 methylation of the title purine nucleotides was achieved in near neutral aqueous solution by reaction with (13)C enriched [Co(NH(3))(5)(CH(3))](2+). This cation decomposes upon dissolution in water by release of a methyl radical. The latter was identified by electron spin resonance (ESR) spectroscopy as the PBN/(13)C(.-)H(3) adduct (PBN=phenyl-N-tert-butylnitrone). When the purine nucleotide was used in large excess, the efficiency of the trapping by the C8 atom was determined by integration of the (13)C NMR signals to be 20-25% for GMP and 15-20% for AMP, respectively, at 37 degrees C.
Kofod
Pauli
P
Department of Life Sciences and Chemistry, Roskilde University, P.O. Box 260, DK-4000 Roskilde, Denmark. pkofod@ruc.dk
D000249:Adenosine Monophosphate / Q000737:chemistry*; D005609:Free Radicals; D006157:Guanosine Monophosphate / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000737:chemistry*
D005609:Free Radicals; D009942:Organometallic Compounds; C504264:pentaamminemethylcobalt(III); D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate