Poliovirus sampling by using sodium dodecyl sulfate/EDTA-pretreated chromatography paper strips.
To achieve the goal of poliovirus eradication, surveillance of endemic areas is a crucial step in the poliovirus eradication program. Currently, six countries still have endemic poliovirus. We have tested a novel method which uses SDS/EDTA-treated chromatography paper strips to collect and transport poliovirus-containing stool samples. The SDS/EDTA-treated paper strips were soaked with different dilutions of poliovirus-containing feces and stored at different temperatures. After storing the SDS/EDTA paper strips for 5 months at 37 degrees C, poliovirus RNA could be successfully amplified using RT-PCR. Infectivity of wild-type poliovirus type 1, 2, and 3 was lost upon contact with the SDS/EDTA-treated strips. This easy, inexpensive, and biosafe chromatography paper strip method for the collection and transportation of poliovirus samples can be of use in poliovirus surveillance and polio vaccination programs.
Maes
Piet
P
Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Belgium.
Van Doren
Els
E
Denys
Barbara
B
Thoelen
Inge
I
Rahman
Mustafizur
M
Vijgen
Leen
L
Van Ranst
Marc
M
D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D004492:Edetic Acid; D005243:Feces / Q000821:virology*; D010209:Paper; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid