Evaluation of the antiherpetic activity of standardized extracts of Achyrocline satureioides.
Traditionally, Achyrocline satureioides or 'marcela' has been used in South America for the treatment of several disorders. For the present study, three spray-dried extracts (N1, N2 and N3) were used, all of them prepared with 50% of an hydroethanolic extract rich in flavonoid compounds and 50% of blends of different adjuvants. The cytotoxic concentration which causes destruction in 50% monolayer cells (CC50) was 62.5 microg/ml for the three extracts. The antiviral activity was evaluated by using two different strains of herpes simplex virus (HSV-1) and the best results were obtained with KOS strain and N2 extract. Studies concerning the mechanism of the antiherpetic activity demonstrated that N2 extracts showed no virucidal effect or activity on cellular receptors. HSV-1 DNA synthesis was not inhibited. The antiherpetic activity occurred between the second and ninth hour of the virus replication cycle, probably indicating a perturbation on late stages of this cycle.
Bettega
J M R
JM
Laboratório de Virologia Aplicada Departamento de Ciências Farmacêuticas (CIF/CCS), Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Teixeira
H
H
Bassani
V L
VL
Barardi
C R M
CR
Simões
C M O
CM
D036921:Achyrocline; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D006561:Herpes Simplex / Q000188:drug therapy; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D016133:Polymerase Chain Reaction; D014709:Vero Cells / Q000187:drug effects
D000998:Antiviral Agents; D017931:DNA Primers; D004279:DNA, Viral; D010936:Plant Extracts