Potential of DNA microarrays for developing parallel detection tools (PDTs) for microorganisms relevant to biodefense and related research needs.
Development of parallel detection tools using microarrays is critically reviewed in view of the need for screening multiple microorganisms in a single test. Potential research needs with respect to probe design and specificity, validation, sample concentration, selective target enrichment and amplification, and data analysis are discussed. Data illustrating selected probe design issues for detecting multiple targets in mixed microbial systems is presented. Challenges with respect to cost, time, and ease of use compared to other methods are also summarized.
Hashsham
Syed A
SA
Department of Civil and Environmental Engineering, Michigan State University, A 126 Research Complex-Engineering, East Lansing, MI 48824, USA. hashsham@egr.msu.edu
Wick
Lukas M
LM
Rouillard
Jean-Marie
JM
Gulari
Erdogan
E
Tiedje
James M
JM
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001694:Biological Warfare / Q000517:prevention & control; D023482:Bioterrorism / Q000517:prevention & control*; D002959:Civil Defense / Q000295:instrumentation / Q000379:methods / Q000639:trends; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D056890:Eukaryota / Q000302:isolation & purification*; D005658:Fungi / Q000302:isolation & purification*; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods / Q000639:trends; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D012106:Research / Q000295:instrumentation* / Q000639:trends; D012107:Research Design; D018511:Systems Integration; D013673:Technology Assessment, Biomedical