title: An enzyme-linked immunosorbent assay for human carcinoembryonic antigen-related cell adhesion molecule 8, a biological marker of granulocyte activities in vivo.
abstract: Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, NCA-95 and CD67, is a highly glycosylated protein expressed only in neutrophils and eosinophils in humans. The precise function of CEACAM8 remains unclear. As a member of the family of carcinoembryonic antigen (CEA), it may play a role in the interaction between granulocytes or between granulocytes and epithelial cells. We describe here an accurate, specific and reproducible enzyme-linked immunosorbent assay (ELISA) using purified native CEACAM8 as standard for the measurement of CEACAM8 with a detection range of 1-64 microg/l. We also present data on the levels of CEACAM8 in the blood of healthy individuals and patients undergoing surgery, as well as in patients with acute infections. The highly elevated levels of CEACAM8 in the blood of these patients, which are significantly correlated with the surface expression of CEACAM8 on neutrophils and the number of circulating neutrophils, suggest that CEACAM8 could serve as a biological marker for granulocyte activities in vivo.
authors.affiliation: Department of Medical Sciences, Clinical Chemistry, Uppsala University, S-751 85, Uppsala, Sweden. linshu.zhao@medsci.uu.se
authors.forename: Linshu
authors.initials: L
authors.lastname: Zhao
authors.forename: Shengyuan
authors.initials: S
authors.lastname: Xu
authors.forename: Gustav
authors.initials: G
authors.lastname: Fjaertoft
authors.forename: Karlis
authors.initials: K
authors.lastname: Pauksen
authors.forename: Lena
authors.initials: L
authors.lastname: Håkansson
authors.forename: Per
authors.initials: P
authors.lastname: Venge
mesh_terms: D015703:Antigens, CD; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000097:blood / Q000276:immunology; D015415:Biomarkers; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000097:blood / Q000276:immunology; D019411:Clinical Laboratory Techniques; D003141:Communicable Diseases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D058851:GPI-Linked Proteins; D006098:Granulocytes / Q000276:immunology*; D006801:Humans
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins