Development of a psilocin immunoassay for serum and blood samples.
After the immunisation of rabbits with a psilocin-specific immunogen, polyclonal antisera were obtained. With these antisera a competitive, heterogeneous radioimmunoassay for the detection of psilocin was developed. As tracer a derivative of psilocin was synthesised, which contained a tritiated CH(3) group. The antisera showed a specific reaction with psilocin. The cross-reactivity of structurally related endogenous substances like serotonin, tryptophan and tyrosine was below 0.01%. Also common drugs of abuse (Delta(9)-tetrahydrocannabinol, cocaine, morphine, amphetamine) showed negligible cross-reactivity (0.01-2%). Only tricyclic neuroleptics with a (dimethylamino)ethyl side-chain showed some cross-reactivity (20%). Spiked serum and blood samples were analysed with this new immunoassay and the results obtained were compared with the values measured with a validated GC-MS method.
Albers
C
C
Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Hittorfstrasse 58-62, 48149 Münster, Germany.
Köhler
H
H
Lehr
M
M
Brinkmann
B
B
Beike
J
J
D000818:Animals; D006213:Hallucinogens / Q000097:blood*; D006801:Humans; D011562:Psilocybin / Q000031:analogs & derivatives* / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
D006213:Hallucinogens; D011562:Psilocybin; C009105:psilocin