title: Molecular and functional analysis of the dictyostelium centrosome.
abstract: The centrosome is a nonmembranous, nucleus-associated organelle that functions not only as the main microtubule-organizing center but also as a cell cycle control unit. How the approximately 100 different proteins that make up a centrosome contribute to centrosome function is still largely unknown. Considerable progress in the understanding of centrosomal functions can be expected from comparative cell biology of morphologically different centrosomal structures fulfilling conserved functions. Dictyostelium is an alternative model organism for centrosome research in addition to yeast and animal cells. With the elucidation of morphological changes and dynamics of centrosome duplication, the establishment of a centrosome isolation protocol, and the identification of many centrosomal components, there is a solid basis for understanding the biogenesis and function of this fascinating organelle. Here we give an overview of the prospective protein inventory of the Dictyostelium centrosome based on database searches. Moreover, we focus on the comparative cell biology of known components of the Dictyostelium centrosome including the gamma-tubulin complex and the homologues of centrin, Nek2, XMAP215, and EB1.
authors.affiliation: Adolf-Butenandt-Institut?Zellbiologie, Universität München, D-80336 München, Germany.
authors.forename: Ralph
authors.initials: R
authors.lastname: Gräf
authors.forename: Christine
authors.initials: C
authors.lastname: Daunderer
authors.forename: Irene
authors.initials: I
authors.lastname: Schulz
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D018385:Centrosome / Q000378:metabolism* / Q000648:ultrastructure; D048749:Cytokinesis / Q000502:physiology; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D022101:Microtubule-Organizing Center / Q000378:metabolism* / Q000648:ultrastructure; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism
chemical_list: C486738:CKAP5 protein, Xenopus; C405804:EB1 microtubule binding proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D029867:Xenopus Proteins; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D000072379:NIMA-Related Kinases; C490317:Nek2 protein, mouse; D017346:Protein Serine-Threonine Kinases