Anesthetized Long Evans rats show similar protein expression and long-term potentiation as Fischer 344 rats but reduced short-term potentiation in motor cortex.
A number of studies describe strain-related differences in the motor behavior of rats. Inbred albino F344 rats are found to be impaired in procedural spatial learning, skilled reaching, and over ground locomotion in relation to pigmented out bred Long Evans (LE) rats. These deficits could be related to the functional differences in the motor cortex of the two strains, and the objective of the present study was to examine this hypothesis. Synaptic transmission was examined in the two rat strains, using long-term potentiation (LTP) and short-term potentiation (STP), two electrophysiological measures of neural function and learning. Field potentials were evoked in the motor cortex of anesthetized Long Evans and Fischer 344 (F344) rats in response to contralateral white matter stimulation. The main findings indicated that (1) baseline-evoked responses in the two strains was similar, indicating similar basal levels of synaptic strength, (2) LTP was induced in both strains of rats, suggesting similar synaptic efficacy in the two strains of rats, and (3) STP was enhanced in the Fischer 344 rats, suggesting differences in synaptic function. Protein expression also revealed that the two strains did not differ with respect to structural or synaptic protein expression. Thus, the two strains exhibit motor skill differences despite a great degree of physiological similarity in motor cortex. The results are discussed in relation to the greater utility of using the Long Evans rat for examining the neural basis of plasticity and models of disease, especially if motor tasks are evaluated.
Wawryko
Paul
P
Department of Psychology, University of Manitoba, Winnipeg, Manitoba, Canada R3T-2N2.
Ward
Nicole L
NL
Whishaw
Ian Q
IQ
Ivanco
Tammy L
TL
D000704:Analysis of Variance; D000758:Anesthesia; D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology; D019054:Evoked Potentials, Motor / Q000235:genetics / Q000502:physiology; D051860:Fragile X Messenger Ribonucleoprotein 1; D006993:Hypnotics and Sedatives / Q000008:administration & dosage; D007267:Injections; D017774:Long-Term Potentiation / Q000235:genetics / Q000502:physiology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D023421:Models, Animal; D009044:Motor Cortex / Q000502:physiology*; D009048:Motor Skills / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D010424:Pentobarbital / Q000008:administration & dosage; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344 / Q000502:physiology*; D020318:Rats, Long-Evans / Q000502:physiology*; D013045:Species Specificity; D009435:Synaptic Transmission / Q000235:genetics / Q000502:physiology*; D016708:Synaptophysin / Q000096:biosynthesis
D051860:Fragile X Messenger Ribonucleoprotein 1; D006993:Hypnotics and Sedatives; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010424:Pentobarbital; D016601:RNA-Binding Proteins; D016708:Synaptophysin; C497685:Fmr1 protein, rat