title: Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification.
abstract: We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Horisaka
authors.forename: Kayoko
authors.initials: K
authors.lastname: Fujita
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Iwata
authors.forename: Aya
authors.initials: A
authors.lastname: Nakadai
authors.forename: Alexandre T
authors.initials: AT
authors.lastname: Okatani
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Horikita
authors.forename: Takahide
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Honda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashidani
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D018420:Ape Diseases / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D008992:Monkey Diseases / Q000175:diagnosis / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011148:Pongo pygmaeus; D012453:Saimiri; D012680:Sensitivity and Specificity; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D018829:Adhesins, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial