On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin.
Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on-line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on-line cation exchange in order to prevent massive adducting in the ESI interface.
Henriksen
Jens
J
Department of Spectroscopy, LEO Pharma A/S, 2750 Ballerup, Denmark. jens.henriksen@leo-pharma.com
Ringborg
Lene Hoffmeyer
LH
Roepstorrf
Peter
P
D000107:Acetylation; D002412:Cations / Q000737:chemistry; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005343:Fibrinolytic Agents / Q000032:analysis* / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000032:analysis* / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000032:analysis / Q000737:chemistry; D000078222:Tinzaparin
D002412:Cations; D005343:Fibrinolytic Agents; D006495:Heparin, Low-Molecular-Weight; D009844:Oligosaccharides; D013431:Sulfates; D000078222:Tinzaparin