title: Differences in the expression of LPS-receptors are not responsible for the sex-specific immune response after trauma and hemorrhagic shock.
abstract: Several studies demonstrated a sex-specific cytokine secretion by macrophages following trauma-hemorrhage (T-H) and incubation with lipopolysaccharide A (LPS). Although LPS is known to act via the receptors CD14 and TLR4 on macrophages, it remains unknown whether differences in LPS receptor expression in males and females may be responsible for the gender-specific LPS induced cytokine response following (T-H). To study this, male and proestrus female mice (C3H/HeN) were subjected to trauma (laparotomy) followed by hemorrhage or sham operation. At 2 h thereafter, SMphi and PMphi were harvested and cultured for 2 h. The expression of CD14 and TLR4 was measured by flow cytometry on unstimulated SMphi and PMphi as well as after LPS stimulation. The results indicate that the expression of CD14 and TLR4 on SMphi and PMphi from female and male mice was similar in sham-operated animals and after (T-H). Incubation of macrophages with LPS did not alter CD14 and TLR4 expression in the study groups. Thus, the sex specific LPS induced cytokine secretion after (T-H) is not caused by differences in LPS receptor expression on Mphi of male and female mice.
authors.affiliation: Department of Surgery, Klinikum Grosshadern Ludwig-Maximilians University, Marchioninistr. 15, D-81377 Munich, Germany.
authors.forename: S J
authors.initials: SJ
authors.lastname: Eisenmenger
authors.forename: M W
authors.initials: MW
authors.lastname: Wichmann
authors.forename: P
authors.initials: P
authors.lastname: Angele
authors.forename: E
authors.initials: E
authors.lastname: Faist
authors.forename: R
authors.initials: R
authors.lastname: Hatz
authors.forename: I H
authors.initials: IH
authors.lastname: Chaudry
authors.forename: K W
authors.initials: KW
authors.lastname: Jauch
authors.forename: M K
authors.initials: MK
authors.lastname: Angele
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D018950:Lipopolysaccharide Receptors / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011359:Proestrus; D012727:Sex Characteristics; D012771:Shock, Hemorrhagic / Q000150:complications / Q000276:immunology* / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D014947:Wounds and Injuries / Q000150:complications / Q000276:immunology* / Q000378:metabolism*
chemical_list: D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides