title: Effects of amino acids and its metabolites on prolidase activity against various iminodipeptides in erythrocytes from normal human and a patient with prolidase deficiency.
abstract: BACKGROUND
  The characteristics of prolidase in erythrocytes from controls and patient with prolidase deficiency were investigated.
  
  
  METHODS
  The erythrocytes were isolated from the heparinized blood of normal human and a patient with prolidase deficiency. Effects of various amino acids and their metabolites on prolidase activity against iminodipeptides in presence of 1 mmol/l MnCl(2) were investigated.
  
  
  RESULTS
  Prolidase activity against glycylproline in erythrocytes from normal human was strongly enhanced by glycine, L-alanine, L-serine with MnCl(2), but the activity was strongly inhibited by L-valine, and L-leucine. However, the stereoisomers, D-leucine and D-valine enhanced the activity. The prolidase activity against methionylproline in erythrocytes from the patient with prolidase deficiency was also enhanced by glycine, L-alanine and L-serine. The activity was inhibited by l-leucine, but D-leucine and L-valine enhanced the activity against various iminodipeptides.
  
  
  CONCLUSIONS
  Prolidase activity against glycylproline in normal human erythrocytes and against methionylproline from the prolidase-deficient patient was enhanced strongly by glycine, alanine and serine with MnCl(2). However, this activity was inhibited by L-leucine, but was enhanced by D-leucine.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi 783-8505, Japan.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nakayama
authors.forename: Shiro
authors.initials: S
authors.lastname: Awata
authors.forename: Weifang
authors.initials: W
authors.lastname: Wang
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Masanobu
authors.initials: M
authors.lastname: Manabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kodama
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D002712:Chlorides / Q000097:blood / Q000378:metabolism; D004150:Dipeptidases / Q000172:deficiency / Q000378:metabolism*; D004151:Dipeptides / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D007098:Imino Acids / Q000378:metabolism; D017895:Manganese Compounds / Q000097:blood / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D002712:Chlorides; D004151:Dipeptides; D007098:Imino Acids; D017895:Manganese Compounds; D004150:Dipeptidases; C022449:proline dipeptidase; C025340:manganese chloride