Knockdown of mouse adult beta-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference.
RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21-23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especially when it is necessary to generate long-term gene silencing in vivo. Virus vector-mediated RNAi provides an alternative to transfection. In the present study, we investigated such transduction system and showed that retrovirus vector-mediated RNAi can substantially down-regulate expression of mouse adult beta-globin gene in MEL cells. The results suggest that retrovirus vector-delivered RNAi may find its use in functional genomics and in gene therapy.
Zhao
Na
N
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC), 5 Dong Dan San Tiao, Beijing 100005, PR China.
Zu
Zhen-Xiang
ZX
Liu
Chang-Mei
CM
Dong
Wen-Ji
WJ
Liu
De-Pei
DP
Liang
Chih-Chuan
CC
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D034622:RNA Interference; D012190:Retroviridae / Q000235:genetics*
D005914:Globins