A novel vector for the expression of SCR domains in insect cells.
Exploitation of recombinant technology to study proteins containing strings of short consensus repeat (SCR) domains largely depends on expression vectors. In this paper, we describe a vector for cloning and constitutive expression of single or multiple SCR domains. The recombinant vector has unique additive features over commercially available vectors that make it a universal cloning vector for SCR domains as well as a vector suitable for expressing any protein fragment beginning and ending with cysteine residues. As a demonstration of its usefulness, the constitutive extracellular expression of five SCR-containing proteins derived from complement factor H is presented.
Alam
M Nurul
MN
Department of Biochemistry, University of Texas Health Science Center, 11937 U.S. Hwy 271, Tyler, TX 75703, USA.
Haque
Aftabul
A
Sreedhar
Maladi
M
Pangburn
Michael K
MK
D000818:Animals; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D007313:Insecta / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D017434:Protein Structure, Tertiary / Q000235:genetics* / Q000502:physiology; D014162:Transfection
D017242:Complement Factor H