title: Hyperosmotic chemical agent's effect on in vivo cerebral blood flow revealed by laser speckle.
abstract: We investigated the influence of a hyperosmotic agent (glycerol) on the normal physiological function of tissue by applying the glycerol in vitro and in vivo to rabbit dura mater to assess the changes in the tissue's optical properties. We used a laser speckle imaging technique to study the effect of epidurally applied glycerol on resting cerebral blood flow (CBF). Our results showed that resting CBF decreased as the transparency of the dura mater increased. The challenges for the design of an optical clearing technique were not only the clearing effects and the duration of the action of the chemical agents but also the influence of the glycerol on the tissue's normal physiological function.
authors.affiliation: The Key Laboratory of Biomedical Photonics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Cheng
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Shangbin
authors.initials: S
authors.lastname: Chen
authors.forename: Weihua
authors.initials: W
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
mesh_terms: D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D004388:Dura Mater / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology*; D005990:Glycerol / Q000494:pharmacology; D017078:Laser-Doppler Flowmetry / Q000379:methods; D007834:Lasers; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011817:Rabbits; D012542:Scattering, Radiation
chemical_list: D005990:Glycerol