Adrenocortical response to stress in fasted and unfasted artificially reared 12-day-old rat pups.
Recent studies have compared artificially reared (AR) rats with dam-reared rats on behavioral outcomes but, despite the fact that they are deprived of their mothers during the stress hyporesponsive period (SHRP), little is known about the effects of AR on the development of the stress response. In this study, the corticosterone (CORT) response to a stressor (saline injection ip) on postnatal Day 12 was assessed in rat pups that had been either dam-reared (DR) or artificially reared since Day 5. In the preceding 24 hr, half the pups in the DR group were maternally deprived (DEP). To control for the food deprivation consequent to maternal deprivation in these groups, half the pups in the AR groups also underwent 24-hr food deprivation (DEP). In the nondeprived condition AR pups did not differ from DR pups on untreated CORT levels or on levels at 1-hr poststress (i.e., all rats demonstrated low levels of CORT characteristic of the SHRP). In contrast, both maternally deprived DR pups and food-deprived AR pups exhibited increased untreated CORT levels as well as a significant increase at 30-min poststress, but CORT elevations were lower in the AR groups than in the DR groups. Thus, long-term maternal deprivation through artificial rearing in rats does not affect the reduced CORT levels and reduced CORT responsiveness associated with the SHRP; however, if animals are food deprived, then all show increased basal CORT levels and a greater CORT response to stress, although this response is lower in AR groups than in DR groups. These results suggest that rat pups artificially reared with adequate nutrition will still exhibit the SHRP.
Ward
Glenn
G
Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1. gward@healthy.uwaterloo.ca
Xing
Hua Cheng
HC
Carnide
Nancy
N
Slivchak
Jane
J
Wainwright
Patricia
P
D000818:Animals; D000831:Animals, Newborn; D003345:Corticosterone / Q000378:metabolism*; D005215:Fasting; D005260:Female; D005508:Food Deprivation; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D008426:Maternal Deprivation; D010913:Pituitary-Adrenal System / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D020318:Rats, Long-Evans; D013315:Stress, Psychological / Q000378:metabolism*; D014886:Weaning
D003345:Corticosterone