Real-time three-dimensional optical micromanipulation of multiple particles and living cells.
Counterpropagating light fields provide a stationary optical potential well for a Brownian particle. Introducing variability in the relative strengths of the counterpropagating beams allows us to create a more general configuration-the optical elevator. An optical elevator dynamically controls the axial location of the potential minimum where the particle finds a stable equilibrium position. We describe the implementation of multiple real-time reconfigurable optical elevators with the generalized phase contrast method for dynamic manipulation of polystyrene spheres and yeast cells S. cerevisiae in three dimensions.
Rodrigo
Peter John
PJ
Optics and Plasma Research Department, Risø National Laboratory, Roskilde, Denmark.
Daria
Vincent Ricardo
VR
Glückstad
Jesper
J
D002465:Cell Movement / Q000528:radiation effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D009038:Motion; D009862:Online Systems / Q000295:instrumentation; D055095:Optics and Photonics / Q000295:instrumentation*; D010316:Particle Size; D010812:Physical Stimulation / Q000295:instrumentation* / Q000379:methods; D011137:Polystyrenes / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*
D011137:Polystyrenes