[Urinary 8-hydroxy-2'-deoxyguanosin (8-OHdG) in patients with chronic liver diseases].
Urinary 8-hydroxy-2'-deoxyguanosin(8-OHdG) has been reported as sensitive biomarker of oxidative DNA damage and also of oxidative stress. We measured the urinary 8-OHdG in patients with chronic liver diseases by competitive ELISA, and analyzed the relationship with clinical characteristics. Fifty patients (male/female: 22/28) with chronic liver disease were enrolled this study. The mean concentration of urinary 8-OHdG in healthy control and patients with liver cirrhosis, chronic hepatitis C, chronic hepatitis B, and autoimmune hepatitis were 10.40+/-3.14, 10.14+/-4.19, 11.79+/-5.58, 14.99+/-4.46, and 13.64+/-3.84 microg/gCr, respectively. There were no significant differences among the five group. The mean concentration of urinary 8-OHdG in inveterate drinker was significantly higher than that in non-drinker (16.67+/-4.29 vs. 11.19+/-4.80 microg/gCr, p<0.05). The smoking enhanced the elevation of urinary 8-OHdG in drinkers. In clinical characteristics, serum y-GTP, a marker of alcoholic liver disease, had significant positive correlation with urinary 8-OHdG on the drinker with chronic hepatitis. In addition, there was a positive correlation between serum ferritin levels and urinary 8-OHdG levels. Iron in the liver suggested oxidative damage of hepatocytes through the fenton reaction in patients with chronic liver disease. In conclusion, drinking and smoking induced liver damage by oxidative stress, and urinary 8-OHdG may be reliable marker of oxidative stress in patients with chronic liver disease.
Ishizakai
Miki
M
School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi.
Yoshida
Kensuke
K
Nishimoto
Natsumi
N
Saleh
Al-Ma'Quol Wedad Hamdi
AM
Ishii
Chiaki
C
Handa
Hiroshi
H
Ogawara
Hatsue
H
Nagamine
Takeaki
T
Murakami
Masami
M
Murakami
Hirokazu
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects*; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002908:Chronic Disease; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005293:Ferritins / Q000097:blood; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis* / Q000209:etiology* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012907:Smoking / Q000009:adverse effects; D005723:gamma-Glutamyltransferase / Q000097:blood
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005293:Ferritins; D005723:gamma-Glutamyltransferase; D003849:Deoxyguanosine