Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the chinese medicinal herb Rheum officinale Baill. by high-speed counter-current chromatography.
A high-speed counter-current chromatography (HSCCC) method for preparative separation and purification of five hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. was developed by using pH-gradient elution. The purities of rhein, emodin, aloe-emodin, chrysophanol, physcion and cinnamic acid were all over 98%, as determined by high performance liquid chromatography (HPLC). The structures of them were identified by 1H NMR.
Liu
Renmin
R
Department of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, China. renminliu@lctu.edu.cn
Li
Aifeng
A
Sun
Ailing
A
D000880:Anthraquinones / Q000302:isolation & purification*; D002934:Cinnamates / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards; D012250:Rheum / Q000737:chemistry*
D000880:Anthraquinones; D002934:Cinnamates; C029010:cinnamic acid