Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy.
Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.
Park
Taehan
T
Department of Chemistry, Hanyang University, Ansan 426-791, Korea.
Lee
Moonkwon
M
Choo
Jaebum
J
Kim
Yang S
YS
Lee
Eun Kyu
EK
Kim
Duck Joong
DJ
Lee
Sang-Hoon
SH
D019840:2-Propanol / Q000032:analysis / Q000737:chemistry; D004129:Dimethylpolysiloxanes; D000431:Ethanol / Q000032:analysis / Q000737:chemistry; D008832:Microchemistry / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D012828:Silicones; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
D004129:Dimethylpolysiloxanes; D012828:Silicones; D000431:Ethanol; C013830:baysilon; D019840:2-Propanol