title: Flow injection-chemiluminescence determination of puerarin in pharmaceutical preparations.
abstract: Strong chemiluminescence was observed when cerium(IV) reacted with rhodamine 6G in sulfuric acid medium in the presence of puerarin. This phenomenon has been utilized to design a sensitive and selective flow injection-chemiluminescence method for the determination of puerarin. Under the optimum conditions, the proposed procedure has a linear range between 1.3 x 10(-9) and 8.0 x 10(-7)g/mL, with a detection limit of 8.4 x 10(-10)g/mL puerarin and a relative standard deviation of 1.86% (n = 11) at 5.0 x 10(-8)g/mL puerarin. The method was successfully applied to the determination of puerarin in pharmaceutical preparations. The mechanism of this chemiluminescence reaction has been proposed.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026, PR China.
authors.forename: Qunlin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Aung
authors.initials: A
authors.lastname: Myint
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.forename: Xuewu
authors.initials: X
authors.lastname: Ge
authors.forename: Hua
authors.initials: H
authors.lastname: Cui
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry
chemical_list: D007529:Isoflavones; D004364:Pharmaceutical Preparations; C033607:puerarin