Fluorescence quenching of parallel-stranded DNA bound ethidium bromide: the effect of 7-deaza-2'-deoxyisoguanosine and 7-halogenated derivatives.
Parallel stranded (ps) duplexes were constructed by incorporating 7-deaza-2'-deoxyisoguanosine (1a) or its 7-halogenated analogs 1b,c in place of 2'-deoxyisoguanosine. UV and Tm analyses prove the high affinity of ethidium bromide (EB) to these modified duplexes. Steady-state fluorescence measurement shows that the fluorescence is quenched when EB is bound to ps duplexes containing compounds 1a-c. The quenching effect depends on the 7-substituent of the nucleobase.
Li
Hong
H
Laboratorium für Organische und Bioorganische Chemie, Institut für Chemie, Universität Osnabrück, Barbarastrasse 7, D-49069 Osnabrück, Germany.
Peng
Xiaohua
X
Seela
Frank
F
D020029:Base Pairing; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence / Q000379:methods
C045550:7-deaza-2'-deoxyguanosine; D004247:DNA; D004996:Ethidium; D003849:Deoxyguanosine