Synthesis of phosphate-branched oligonucleotides.
A solid-phase synthesis for phosphate-branched oligonucleotides is described. The method is based on coupling of a single nucleoside phosphorodiamidite to terminal hydroxyl functions of two solid-supported oligonucleotides. After oxidation of the phosphite triester obtained to a phosphate triester, the third branch is assembled by conventional phosphoramidite chemistry.
Heinonen
Petri
P
Department of Chemistry, University of Turku, FIN-20014 Turku, Finland. petheino@utu.fi
Lönnberg
Harri
H
D009841:Oligonucleotides / Q000032:analysis / Q000138:chemical synthesis*; D010710:Phosphates / Q000032:analysis / Q000138:chemical synthesis*
D009841:Oligonucleotides; D010710:Phosphates