title: Expression of inhibitory KIR is confined to CD8+ effector T cells and limits their proliferative capacity.
abstract: A subset of effector/memory CD8(+) T cells expresses natural killer cell receptors (NKR). Expression of inhibitory NKR at that stage of T cell differentiation is poorly understood. Interestingly, recent studies in mice indicated that transgenic expression of an inhibitory NKR induced the accumulation of memory T cells by inhibiting activation-induced cell death (AICD). To further understand the role of inhibitory NKR on T cells, we characterized the subset of human peripheral T cells expressing the inhibitory NKR, CD158b, and studied the modulation of antigen-driven T cell expansion by an endogenous inhibitory NKR. We found that CD158b expression was confined to a population of CD8(+)TCRalphabeta(+) effector T cells as defined by a CD45RA(+)CCR7(-) phenotype and high constitutive expression of granzyme B1. Few cells expressed the activating form CD158j in the absence of CD158b. Functionally, engagement of CD158b by MHC ligands diminished early TCR signaling, as well as AICD. However, the reduced AICD did not rescue cells for proliferation, since T cell expansion in the presence of CD158b triggering was impaired. Expression of inhibitory NKR on effector CD8(+) T cells may explain in part the poor replicative capacity of T cells at that stage of differentiation.
authors.affiliation: Division of Immunology and Allergology, University Hospital, Geneva, Switzerland.
authors.forename: Lionel
authors.initials: L
authors.lastname: Arlettaz
authors.forename: Sylvie
authors.initials: S
authors.lastname: Degermann
authors.forename: Casimir
authors.initials: C
authors.lastname: De Rham
authors.forename: Eddy
authors.initials: E
authors.lastname: Roosnek
authors.forename: Bertrand
authors.initials: B
authors.lastname: Huard
mesh_terms: D017209:Apoptosis / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3