[N-acetyl-beta-D-hexosaminidase secreted by the marine fungus Phoma glomerata].
Among the ten strains of marine fungi studied, the mycelial fungus Phoma glomerata showed maximum potency in producing N-acetyl-beta-D-glucosaminidase. The conditions for fungal growth and enzyme biosynthesis were evaluated. N-acetyl-beta-D-hexosaminidase was isolated from the culture liquid of Phoma glomerata by ion-exchange chromatography (on DEAE-cellulose and DEAE-Sephacell) and gel filtration (on Toyopearl HW-55) with a yield of 35%; the enzyme, purified 36.4-fold, had a molecular weight of 20 kDa. The homogeneity of the enzyme was confirmed by gel filtration and SDS-PAGE. Transglycosylation reactions catalyzed by the enzyme produced N-acetyl-D-glucosamine and N-acetyl-D-galactosamine with respective yields of 38 and 46%.
Zhuravleva
N V
NV
Luk'ianov
P A
PA
Pivkin
M V
MV
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D006031:Glycosylation; D008386:Marine Biology; D008970:Molecular Weight; D001619:beta-N-Acetylhexosaminidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D001619:beta-N-Acetylhexosaminidases