Effect of dexamethasone on moesin gene expression in rabbit bone marrow stromal cells.
The influence of dexamethasone on rabbit bone marrow stromal cells differentiation was studied by screening the action of dexamethasone on gene expression. Using differential display, we observed some differential amplifications. The use of five of thirteen different primers combination allowed to identify one or more differential bands. One of them was identified as moesin gene. Real-time PCR confirmed a significant reduction of moesin gene expression following dexamethasone treatment. The decrease of expression for this protein, involved in cytoskeletal organization, could explain the effects of dexamethasone treatment on bone marrow stromal cells differentiation.
Cornet
F
F
Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies (LR2B), Université du Littoral Côte d'Opale, Bassin Napoleon, Quai Robert Masset, Boulogne sur mer, France.
Broux
O
O
Anselme
K
K
Hardouin
P
P
Jeanfils
J
J
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D003599:Cytoskeleton / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000494:pharmacology; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000378:metabolism; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology*
D017931:DNA Primers; D018076:DNA, Complementary; D005938:Glucocorticoids; D008840:Microfilament Proteins; D012333:RNA, Messenger; C071058:moesin; D003907:Dexamethasone