title: Simultaneous determination of nitrite, nitrate, thiocyanate and uric acid in human saliva by capillary zone electrophoresis and its application to the study of daily variations.
abstract: Simultaneous determination of nitrite (NO2-), nitrate (NO3-), thiocyanate (SCN-) and uric acid in human saliva was performed by capillary zone electrophoresis using a coated capillary with reversed electroosmotic flow (EOF), using a 100 mM sodium phosphate buffer at pH 6.5 as a running buffer. Saliva samples were deproteinized with acetonitrile and filtered through a membrane filter. The important advantages of the reported method are: simple operation, short analysis time, minimal sample pre-treatment and sample dilution. In order to evaluate the daily variations of the anionic components, the concentrations were determined in the human saliva of four healthy volunteers upon waking and at 2qh intervals during a day.
authors.affiliation: Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan. yoshihide-tanaka@aist.go.jp
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Nahoko
authors.initials: N
authors.lastname: Naruishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fukuya
authors.forename: Jo
authors.initials: J
authors.lastname: Sakata
authors.forename: Keiitsu
authors.initials: K
authors.lastname: Saito
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Wakida
mesh_terms: D002940:Circadian Rhythm; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D013861:Thiocyanates / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis*
chemical_list: D009566:Nitrates; D009573:Nitrites; D013861:Thiocyanates; D014527:Uric Acid; C031760:thiocyanate