title: Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper T cells and prevent autoimmune diabetes in non-obese diabetic mice.
abstract: Compelling evidence suggests that infiltrating CD4+ type I helper T (Th1) cells in the pancreatic islets play a pivotal role in the progression of diabetes in non-obese diabetic (NOD) mice. We demonstrate in the present report that a butanol fraction of B. pilosa suppressed the development of diabetes, helped maintain levels of blood sugar and insulin in NOD mice in a dose-dependent manner and elevated the serum IgE levels regulated by Th2 cytokines in NOD mice. Moreover, the butanol fraction inhibited the differentiation of naive helper T (Th0) cells into Th1 cells but enhanced their transition into type II helper T (Th2) cells using an in vitro T cell differentiation assay. Two polyacetylenic compounds, 2-beta-D-glucopyranosyloxy-1-hydroxy-5(E)-tridecene-7,9,11-triyne and 3-beta-D-glucopyranosyloxy-1-hydroxy-6(E)-tetradecene-8,10,12-triyne, identified from the butanol fraction also prevented the onset of diabetes like the butanol fraction. The latter compound showed a stronger activity for T cell differentiation than the former. In summary, the butanol fraction of B. pilosa and its polyacetylenes can prevent diabetes plausibly via suppressing the differentiation of Th0 cells into Th1 cells and promoting that of Th0 cells into Th2 cells.
authors.affiliation: Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Chang
authors.forename: Cicero Lee-Tian
authors.initials: CL
authors.lastname: Chang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.forename: Rong-Hong
authors.initials: RH
authors.lastname: Hsieh
authors.forename: Chii-Ruey
authors.initials: CR
authors.lastname: Tzeng
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
authors.forename: Huey-Kang
authors.initials: HK
authors.lastname: Sytwu
authors.forename: Lie-Fen
authors.initials: LF
authors.lastname: Shyur
authors.forename: Wen-Chin
authors.initials: WC
authors.lastname: Yang
mesh_terms: D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D031176:Bidens; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007515:Islets of Langerhans / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D008517:Phytotherapy; D011108:Polymers / Q000737:chemistry; D053279:Polyynes; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects*
chemical_list: D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D011108:Polymers; C442780:plant extract, Bidens pilosa; D053279:Polyynes; D000114:Acetylene