title: The human histone gene expression regulator HBP/SLBP is required for histone and DNA synthesis, cell cycle progression and cell proliferation in mitotic cells.
abstract: Histone proteins are essential for chromatin formation, and histone gene expression is coupled to DNA synthesis. In metazoans, the histone RNA binding protein HBP/SLBP is involved in post-transcriptional control of histone gene expression. In vitro assays have demonstrated that human HBP/SLBP is involved in histone mRNA 3' end formation and translation. We have inhibited human HBP/SLBP expression by RNA interference to determine its function during the mitotic cell cycle. Inhibition of HBP/SLBP expression resulted in the inhibition of histone gene expression and DNA synthesis, the inhibition of cell cycle progression in S phase and the inhibition of cell proliferation. These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle.
authors.affiliation: School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Zhao
authors.forename: Susan
authors.initials: S
authors.lastname: McKillop-Smith
authors.forename: Berndt
authors.initials: B
authors.lastname: Müller
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002453:Cell Cycle; D002455:Cell Division; D049109:Cell Proliferation; D004247:DNA / Q000096:biosynthesis* / Q000378:metabolism; D004261:DNA Replication; D005434:Flow Cytometry; D016195:G2 Phase; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D016196:S Phase; D013997:Time Factors; D014162:Transfection; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry / Q000502:physiology*
chemical_list: D006657:Histones; D009687:Nuclear Proteins; C092663:SLBP protein, human; D039221:mRNA Cleavage and Polyadenylation Factors; D004247:DNA