Induction of reversible hemolytic anemia in living zebrafish using a novel small molecule.
We used zebrafish to screen and identify small molecules that affect the process of vertebrate hematopoietic development. Zebrafish embryos were exposed to a library of 5000 synthetic compounds and screened for defects in primitive erythropoiesis. Here, we present the characterization of hemolytic anemia induced in zebrafish by the small molecule 5115318 (3-[5-methyl-furan 2-yl]-propionic acid N'-phenyl-hydrazide). This compound is capable of generating hemoglobin aggregates and Heinz bodies in red cells in vivo only. The induced anemia is reversible and treated fish recover in about 4 days. This study shows the feasibility of using zebrafish to screen for small molecules that can modulate the specific process of erythropoiesis.
Shafizadeh
Ebrahim
E
Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, 621 Charles E. Young Drive, South Life Sciences Building, LS4325, Los Angeles, CA 90095, USA.
Peterson
Randall T
RT
Lin
Shuo
S
D000743:Anemia, Hemolytic / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D041905:Erythroid Cells / Q000187:drug effects / Q000473:pathology; D004920:Erythropoiesis / Q000187:drug effects; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D010659:Phenylhydrazines; D011422:Propionates / Q000737:chemistry / Q000494:pharmacology*; D015027:Zebrafish / Q000502:physiology*
C499096:3-(5-methylfuran 2-yl)propionic acid N'-phenylhydrazide; D005663:Furans; D010659:Phenylhydrazines; D011422:Propionates