title: Immune responses and protective efficacy in ponies immunised with an equine influenza ISCOM vaccine containing an 'American lineage' H3N8 virus.
abstract: Protective responses generated by vaccination with an immuno-stimulating complex (ISCOM)-based vaccine for equine influenza (EQUIP F), containing a new 'American lineage' H3N8 virus, were studied. Seven ponies in the vaccine group received two intramuscular injections of EQUIP F given 6 weeks apart. Aerosol challenge with an A/eq/Newmarket/1/93 reference strain 4 weeks after booster vaccination resulted in clinical signs of infection and viral shedding in 7 influenza-naive control animals whereas the vaccinated ponies were significantly protected from both clinical signs and virus excretion. Influenza virus-specific IgG responses in serum following immunisation with the ISCOM vaccine were predominantly of the IgGa and IgGb sub-isotypes, a pattern similar to that generated by equine influenza virus infection. However, in contrast to the response following infection, virus-specific antibody responses in nasal washes following immunisation were characterised by the presence of IgG but not IgA.These results demonstrated that an ISCOM-based vaccine containing A/eq/Kentucky/98 provides strong protective immunity against challenge with an 'American lineage' H3N8 reference virus.
authors.affiliation: Schering-Plough Animal Health, Breakspear Road South, Harefield, Uxbridge, Middlesex, UB9 6LS, UK. colin.crouch@spcorp.com
authors.forename: C F
authors.initials: CF
authors.lastname: Crouch
authors.forename: J
authors.initials: J
authors.lastname: Daly
authors.forename: D
authors.initials: D
authors.lastname: Hannant
authors.forename: J
authors.initials: J
authors.lastname: Wilkins
authors.forename: M J
authors.initials: MJ
authors.lastname: Francis
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005260:Female; D006461:Hemolysis; D006734:Horse Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology*; D006736:Horses; D016882:ISCOMs / Q000276:immunology*; D007117:Immunization, Secondary / Q000662:veterinary; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D053123:Influenza A Virus, H3N8 Subtype; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D016882:ISCOMs; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines