title: Granulin-like peptide in the mid-gut gland of the bivalve mollusk, Patinopecten yessoensis.
abstract: A cysteine-rich polypeptide, termed CRP1, with a molecular mass of 5829 Da was found to occur in the mid-gut gland of the scallop Patinopecten yessoensis. CRP1 was purified by reverse phase and cation-exchange chromatographies. The amino acid sequence of CRP1 was deduced from its N-terminal amino acid sequence, amino acid composition and the sequence of a partial cDNA, indicating that CRP1 is a 57-amino-acid polypeptide containing 12 cysteine residues with a calculated molecular mass of 5841 Da (5829 Da when oxidized to form six disulfide bridges). A homology search of databases revealed that the deduced amino acid sequence of CRP1 displays significant similarity to those of granulin/epithelins, a family of growth-modulating factors; all cysteine residues in CRP1 are located at the same positions as those conserved characteristically in other known granulin/epithelins. Purified CRP1 inhibited the proliferation of mouse embryo cells. The results suggest that CRP1 functions as a growth-modulating factor in the scallop, and that granulin/epithelin family polypeptides and their precursors play physiologically important roles in invertebrates.
authors.affiliation: Division of Environmental Technology, Aomori Industrial Research Center, 4-11-6 Daini ton-yamachi, Aomori 030-0113, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nara
authors.forename: Hajime
authors.initials: H
authors.lastname: Matsue
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Naraoka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D049109:Cell Proliferation / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003545:Cysteine / Q000737:chemistry; D004064:Digestive System / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D000077153:Progranulins; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D000077153:Progranulins; D003545:Cysteine