Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations.
Analysis of the genetic status of Ki-ras and p53 in primary colorectal carcinomas and matched colorectal liver metastasis from 30 patients reveals an overall heterogeneity both within and between the two tumoral tissues. Both genes were found mutated with a similar frequency in both tissues; however, identical mutations in primary tumor and matched metastasis were found less frequently in the case of the Ki-ras than the p53 gene. Only in three cases the same p53 and Ki-ras mutations found in the primary tumor were found also in the metastasis. In several metastatic specimens the DNA bearing a mutation detected also in the primary tumor appears significantly less abundant than the wild-type DNA. These data are discussed in the light of current models of primary tumor/metastasis relationships.
Albanese
Ida
I
Department of Cellular and Developmental Biology, Universitá di Palermo, Viale delle Scienze, 90128 Palermo, Italy.
Scibetta
Angelo Giuseppe
AG
Migliavacca
Manuela
M
Russo
Antonio
A
Bazan
Viviana
V
Tomasino
Rosa Maria
RM
Colomba
Paolo
P
Tagliavia
Marcello
M
La Farina
Mario
M
D002277:Carcinoma / Q000378:metabolism* / Q000556:secondary*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004252:DNA Mutational Analysis / Q000379:methods; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary*; D009154:Mutation; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013223:Statistics as Topic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53; D015689:Oncogene Protein p21(ras)