Trans-bonded pairs of E-cadherin exhibit a remarkable hierarchy of mechanical strengths.
Classical cadherins are primary mediators of calcium-dependent cell interactions in multicellular organisms. Organized in five tandemly repeated E-cadherin (EC) modules, the extracellular segments of these membrane-spanning glycoproteins interact homophilically between opposing cells to create highly regulated patterns of attachment stabilized by cytoskeletal elements inside the cells. Despite many structural and functional studies, a significant controversy exists in regard to the organization of cadherin binding in adhesion sites. Supported by considerable evidence, perhaps the most widely held view is that opposing N-terminal EC1-EC2 (EC12) domains form a "zipper" of bonds. However, immobilized on two atomically smooth surfaces and pushed to adhesive contact, opposing cadherins become fully interdigitated and unbind through three discrete jumps comparable with domain dimensions when pulled apart. So the question remains as to whether mechanical adhesion strength emanates solely from interactions between the peripheral N-terminal domains or involves multiple overlapping domains. It is also unclear whether a primary adhesion complex is formed by a single opposing pair of cadherins or whether the complex involves a more complicated network of cis-bonded multimers. To address these questions, we used a special jump/ramp mode of force spectroscopy to test isolated pairwise interactions between recombinant fragments of ECs. Besides the formation of strong trans-bonded dimers, we find a remarkable hierarchy of rupture strengths for bonds between the full five-domain fragments that suggests multiple mechanical functions for cadherins, perhaps providing distinct properties needed for transient-specific recognition as well as stable tissue formation.
Perret
E
E
Compartimentation et Dynamiques Cellulaires, Unité Mixte de Recherche 144, Centre National de la Recherche Scientifique-Institut Curie, 75248 Paris, France.
Leung
A
A
Feracci
H
H
Evans
E
E
D000818:Animals; D001483:Base Sequence; D001696:Biomechanical Phenomena; D015820:Cadherins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008956:Models, Chemical; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
D015820:Cadherins; D018076:DNA, Complementary; D010446:Peptide Fragments; D011994:Recombinant Proteins