title: Inhibition of tumor necrosis factor (TNF) signal transduction by the adenovirus group C RID complex involves downregulation of surface levels of TNF receptor 1.
abstract: Adenoviruses employ multiple genes to inhibit the host antiviral responses. There is increasing evidence that these immunoregulatory genes may function either during lytic or latent infection. Adenovirus early transcription region 3 (E3) encodes at least seven proteins, five of which block the acquired or innate immune response. Previous findings from this laboratory demonstrated that the E3 proteins 10.4K and 14.5K, which form a complex in the plasma membrane, inhibit tumor necrosis factor (TNF)-induced activation of NF-kappaB and the synthesis of chemokines. To determine the mechanism of inhibition of these pathways by the adenovirus E3 10.4K/14.5K proteins, we have examined the effects of this viral complex on the inhibition of AP-1 and NF-kappaB activation by TNF and found a reduction in assembly of the TNF receptor 1 (TNFR1) signaling complex at the plasma membrane accompanied by downregulation of surface levels of TNFR1.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, USA.
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Fessler
authors.forename: Y Rebecca
authors.initials: YR
authors.lastname: Chin
authors.forename: Marshall S
authors.initials: MS
authors.lastname: Horwitz
mesh_terms: D017503:Adenovirus E3 Proteins / Q000502:physiology*; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism; D021122:Protein Subunits; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000032:analysis*; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D017503:Adenovirus E3 Proteins; D006982:Hypertonic Solutions; D016328:NF-kappa B; D021122:Protein Subunits; D047888:Receptors, Tumor Necrosis Factor, Type I; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha