Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene.
OBJECTIVE
To test an experimental model of chemical mammary carcinogenesis induction in rats.


METHODS
Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy.


RESULTS
Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis.


CONCLUSIONS
This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
Barros
Alfredo Carlos S D
AC
Discipline of Gynecology, Hospital das Clínicas, Faculty of Medicine, University of São Paulo - São Paulo/SP, Brazil.
Muranaka
Elisa Naomi K
EN
Mori
Lincon Jo
LJ
Pelizon
Christina Helena T
CH
Iriya
Kyoshi
K
Giocondo
Giovana
G
Pinotti
José Aristodemo
JA
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene