title: Combinatorial transcription of herpes simplex virus and varicella zoster virus immediate early genes is strictly determined by the cellular coactivator HCF-1.
abstract: The mammalian transcriptional coactivator host cell factor-1 (HCF-1) functions in concert with Oct-1 and VP16 to assemble the herpes simplex virus (HSV) immediate early (IE) transcription enhancer core complexes that mediate the high level transcription of these genes upon infection. Although this transcriptional model has been well characterized in vitro, the requirements and significance of the components have not been addressed. Oct-1 was previously determined to be critical but not essential for HSV IE gene expression. In contrast, RNA interference-mediated depletion of HCF-1 resulted in abrogation of HSV IE gene expression. The HSV IE gene enhancer domain is a model of combinatorial transcription and consists of the core enhancer and multiple binding sites for factors such as Sp1 and GA-binding protein. It was striking that HCF-1 was strictly required for VP16-mediated transcriptional induction via the core enhancer as well as for basal level transcription mediated by GA-binding protein and Sp1. HCF-1 was also found to be essential for the induction of varicella zoster virus IE gene expression by ORF10, the VZV ortholog of the HSV IE transactivator VP16, and the autostimulatory IE62 protein. The critical dependence upon HCF-1 demonstrates that this cellular component is a key factor for control of HSV and VZV IE gene expression by functioning as the common element for distinct factors cooperating at the IE gene enhancers. The requirements for this protein supports the model whereby the regulated transport of HCF-1 from the cytoplasm to the nucleus in sensory neurons may control IE gene expression and reactivation of these viruses from the latent state.
authors.affiliation: Laboratory of Viral Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Aarthi
authors.initials: A
authors.lastname: Narayanan
authors.forename: Mauricio L
authors.initials: ML
authors.lastname: Nogueira
authors.forename: William T
authors.initials: WT
authors.lastname: Ruyechan
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Kristie
mesh_terms: D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D051268:GA-Binding Protein Transcription Factor; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000502:physiology; D051863:Host Cell Factor C1; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D034622:RNA Interference; D016329:Sp1 Transcription Factor / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D051268:GA-Binding Protein Transcription Factor; C497692:HCFC1 protein, human; D017405:Herpes Simplex Virus Protein Vmw65; D051863:Host Cell Factor C1; C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D016329:Sp1 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; D014759:Viral Envelope Proteins