title: Decreased signs of nicotine withdrawal in mice null for the beta4 nicotinic acetylcholine receptor subunit.
abstract: Withdrawal from chronic exposure to nicotine, the main addictive component of tobacco, produces distinctive symptoms in humans. The appearance of these symptoms is a major deterrent when people try to quit smoking. To study which type of nicotine receptor is relevant for the onset of the withdrawal syndrome, we used a mouse model of nicotine withdrawal. Wild-type mice and mice null for the beta4 (beta4-/-) or the beta2 (beta2-/-) nicotinic acetylcholine receptor subunits were implanted with osmotic minipumps delivering 24 mg x kg(-1) x d(-1) nicotine for 13 d. Subsequently, a single intraperitoneal injection of the nicotinic receptor antagonist mecamylamine induced behavioral symptoms of withdrawal measured as increased grooming, chewing, scratching, and shaking, plus the appearance of some unique behaviors such as jumping, leg tremors, and cage scratching. Mecamylamine injection triggered comparable withdrawal signs in wild-type and in beta2-/- mice, whereas the beta4-/- mice displayed significantly milder somatic symptoms. In addition, nicotine withdrawal produced hyperalgesia in wild-type but not beta4-/- mice. Finally, chronic nicotine produced an increase in epibatidine binding in several areas of the brain in both wild-type and in beta4-/- mice, but such receptor upregulation did not correlate with the severity of withdrawal signs. Our results demonstrate a major role for beta4-containing nicotinic acetylcholine receptors in the appearance of nicotine withdrawal symptoms. In contrast, the beta2 subunit does not seem to greatly influence this phenomenon. We also show that the upregulation of epibatidine binding sites attributable to chronic nicotine, an effect associated with beta2-containing receptors, is probably not related to the mechanisms underlying withdrawal.
authors.affiliation: Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Ramiro
authors.initials: R
authors.lastname: Salas
authors.forename: Fredalina
authors.initials: F
authors.lastname: Pieri
authors.forename: Mariella
authors.initials: M
authors.lastname: De Biasi
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D005260:Female; D005838:Genotype; D006120:Grooming / Q000187:drug effects; D006930:Hyperalgesia / Q000139:chemically induced; D015918:Infusion Pumps, Implantable; D008297:Male; D008409:Mastication / Q000187:drug effects; D008464:Mecamylamine / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009538:Nicotine / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D011725:Pyridines / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D013375:Substance Withdrawal Syndrome / Q000235:genetics* / Q000378:metabolism; D014202:Tremor / Q000139:chemically induced
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D008464:Mecamylamine; D009419:Nerve Tissue Proteins; D009538:Nicotine; D011725:Pyridines; D011978:Receptors, Nicotinic; C486720:Chrnb4 protein, mouse; C082748:epibatidine