title: Poly (AT) polymorphism in intron 11 of the XPC DNA repair gene enhances the risk of lung cancer.
abstract: Reduced DNA repair capacity due to inherited polymorphisms may increase the susceptibility to smoking-related cancers. In this report, we investigate the relationship between xeroderma pigmentosum complementary group C poly (AT) insertion/deletion polymorphism (XPC-PAT) of the XPC gene and lung cancer risk in a hospital-based case-control study of 359 newly diagnosed lung cancer patients and 375 control subjects matched on age, sex, and catchment area. The XPC genotype was determined by PCR-RFLP, and the results were analyzed using logistic regression, adjusting for relevant covariates. We found that the frequency of the PAT+/+ genotype was higher in the cases (20.6%) than in the controls (14.1%; P = 0.057) and that the PAT+/+ subjects were at significantly increased risk for lung cancer [adjusted odds ratio (OR), 1.60; 95% confidence interval (95% CI), 1.01-2.55]. Stratified analysis revealed that the risk was higher in former smokers (OR, 2.15; 95% CI, 1.07-4.31) and older people (OR, 2.76; 95% CI, 1.02-7.51), although this probably occurs due to 63.4% of cases older than 73 years being ex-smokers. When stratified by histologic type, the variant genotype was associated with statistically significant increased risk for squamous cell carcinoma (OR, 1.93; 95% CI, 1.06-3.51). In conclusion, our findings support the hypothesis that PAT and intron 11 C/A XPC polymorphisms are linked in the Spanish population and may contribute to the risk of developing lung cancer probably due to a higher frequency of deletion of exon 12 and reduced DNA repair capacity of the XPC protein.
authors.affiliation: Department of Medicine, Oncology Institute (Molecular Epidemiology Unit of the Oncology Institute), Oviedo University, Edificio Santiago Gascón, 33006 Oviedo (Asturias), Spain.
authors.forename: M Soledad
authors.initials: MS
authors.lastname: Marín
authors.forename: M Felicitas
authors.initials: MF
authors.lastname: López-Cima
authors.forename: Laura
authors.initials: L
authors.lastname: García-Castro
authors.forename: Teresa
authors.initials: T
authors.lastname: Pascual
authors.forename: Manuel G
authors.initials: MG
authors.lastname: Marrón
authors.forename: Adonina
authors.initials: A
authors.lastname: Tardón
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics*; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012907:Smoking / Q000009:adverse effects*; D013030:Spain / Q000453:epidemiology
chemical_list: D004268:DNA-Binding Proteins; C086984:XPC protein, human