title: [Nonviral gene transfection into human dendritic cells by using biodegradable cationized gelatin and plasmid DNA complex].
abstract: Dendritic cells (DC) are potent antigen-presenting cells capable of stimulating T cell mediated immunity. Gene transfer of tumor specific antigens or cytokines into DC would be a useful strategy for immunotherapeutical purposes. In the present study, in vitro transfection of human DCs (hDC) with the complex of biodegradable cationized gelatin and an EGFP gene was performed. Flow cytometric analyses revealed that approximately 14% of DC was positively expressed for EGFP, and the mRNA expression of EGFP gene in transfected hDC was detected by RT-PCR. Additionally, when evaluated by allogeneic MLR, the antigen-presenting capacity of transfected DC was equal to that of control DC. Cationized gelatin is a promising nonviral vector for gene transfer into DC.
authors.affiliation: Dept. of Surgery and Oncology of Digestive System, Graduate School of Medical Science, Kyoto Prefectural University of Medicine.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubota
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takashima
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Atsuji
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Ryuji
authors.initials: R
authors.lastname: Tomoshige
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ueda
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamagishi
mesh_terms: D001673:Biodegradation, Environmental; D002412:Cations; D003713:Dendritic Cells; D004815:Epidermal Growth Factor / Q000032:analysis / Q000235:genetics; D005780:Gelatin; D006801:Humans; D066298:In Vitro Techniques; D011498:Protein Precursors / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D011498:Protein Precursors; D012333:RNA, Messenger; C042652:epidermal growth factor precursor; D004815:Epidermal Growth Factor; D005780:Gelatin