title: [Effect of pyruvate on the dependence of electromechanical activity on stimulation frequency in human myocardium].
abstract: The aim of the study was to determine the effect of pyruvate, a substrate of energetic metabolism, on the dependence of electromechanical activity on stimulation frequency in human myocardium. The experiments were performed on human ventricle myocardium from patients undergoing cardiac corrective open heart surgery at Clinic of Cardiac Surgery in Kaunas University of Medicine by using standard method of registration of myocardium electromechanical activity. The stimulation frequency of myocardial strips were 0.2, 0.5, 1.0, 1.5, 2.0, 2.5 and 3 Hz, duration of pulses -2-5 ms. In control, i.e. at perfusion of mycoardial strips by Tyrode solution and stimulation frequency 1 Hz, an average of contraction force (P) was 0.94+/-0.18 mN (n=12), half time of relaxation (t(a)) - 178.88+/-9.31 ms (n=12), and action potential duration measured at 50% repolarization level (VP(50)) - 294.75+/-27.95 ms (n=9). Pyruvate (10 mM) increased P to 159.9+/-13.4% (p<0.00), t(a)- 106.9+/-2.2% (n=12) (p<0.001), and VP(50)- 111.1+/-7.4% (n=5) (p<0.05), as compared to control. In the absence of pyruvate values of P, t(a) and VP(50) of myocardial strips were higher at 0.2 Hz and 0.5 Hz and lower at 3 Hz compared with those at 1 Hz of stimulation frequency. Although the dependence of these parameters on stimulation frequency in the presence of pyruvate (10 mM) was not altered but their values were persistently increased at all stimulation frequencies. The present data indicate that pyruvate is an effective inotropic agent, which can improve contractility function in wide-ranging diapason of stimulation frequency in failing human heart.
authors.affiliation: Institute of Cardiology, Kaunas University of Medicine, Sukileliu 17, LT-50009 Kaunas, Lithuania. membiof@kmu.lt
authors.forename: Vida
authors.initials: V
authors.lastname: Gendviliene
authors.forename: Danguole
authors.initials: D
authors.lastname: Zablockaite
authors.forename: Agne
authors.initials: A
authors.lastname: Babusyte
authors.forename: Jonas
authors.initials: J
authors.lastname: Jurevicius
authors.forename: Rimantas
authors.initials: R
authors.lastname: Benetis
mesh_terms: D000200:Action Potentials; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002316:Cardiotonic Agents / Q000494:pharmacology*; D005260:Female; D006352:Heart Ventricles / Q000187:drug effects; D006801:Humans; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009200:Myocardial Contraction / Q000187:drug effects*; D010477:Perfusion; D011773:Pyruvates / Q000494:pharmacology*; D012996:Solutions; D016276:Ventricular Function
chemical_list: D002316:Cardiotonic Agents; D011773:Pyruvates; D012996:Solutions