Detection and enumeration of Vibrio vulnificus in oysters from two estuaries along the southwest coast of India, using molecular methods.
This study was conducted to understand the seasonal distribution of Vibrio vulnificus in oysters from two estuaries and the effect of environmental factors on the abundance of V. vulnificus in tropical waters. V. vulnificus was detected in 56.6% of the samples tested by colony hybridization with an alkaline phosphatase-labeled oligonucleotide probe (VV-AP), and the counts ranged from <10/g during the summer months to 10(3)/g in the monsoon season at both sites. The density of V. vulnificus appeared to be controlled more by salinity than by temperature. A nested PCR used in this study detected V. vulnificus in 85% of the samples following 18 h of enrichment in alkaline peptone water.
Parvathi
Ammini
A
Department of Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore, India.
Kumar
H Sanath
HS
Karunasagar
Indrani
I
Karunasagar
Iddya
I
D000362:Agar; D000818:Animals; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis*; D005618:Fresh Water; D007194:India; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012621:Seasons; D012965:Sodium Chloride; D013696:Temperature; D041261:Vibrio vulnificus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*
D003470:Culture Media; D004269:DNA, Bacterial; D012965:Sodium Chloride; D000362:Agar