New polymorphism of FASN gene in chicken.
Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.
Marrube
Graciela
G
Genetics Area, Animal Production Department, Veterinary School, University of Buenos Aires, Chorroarin 280, 1427, Buenos Aires, Argentina. gmarrube@fvet.uba.ar
Rozen
Felisa
F
Pinto
Gabriel Bernardo
GB
Pacienza
Natalia
N
Melo
Julián Enrique
JE
Huguet
Miguel Javier
MJ
Canet
Zulma
Z
Zandomeni
Ruben
R
Miquel
Maria Cristina
MC
D000818:Animals; D002645:Chickens / Q000235:genetics*; D064429:Fatty Acid Synthases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D007438:Introns / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D064429:Fatty Acid Synthases