Structure-activity relationship of lysophosphatidylcholines in HL-60 human leukemia cells.
OBJECTIVE
To explore the structure-activity relationship of lysophosphatidylcholine (LPC) and lysolipid molecules from a marine sponge and ladybirds.


METHODS
We tested three synthetic LPCs and four natural lysolipids on Ca2+ mobilization in HL-60 human leukemia cells.


RESULTS
We observed lysolipid-mediated Ca2+ mobilization. The activity was the same in both ester- and ether-linked lysolipids, and introduction of a double bond or methoxy group on the alkyl chain did not significantly modulate the activity. However, replacement of trimethylammonium moiety in the choline structure with ammonium moiety reduced the activity. Furthermore, change of the alkyl chain length influenced the Ca2+ response.


CONCLUSIONS
LPC-induced Ca2+ mobilization might be dependent on the length of alkyl chain and the presence of choline moiety in HL-60 leukemia cells.
Lee
Eun-hee
EH
Laboratory of Pharmacology, College of Pharmacy, Pusan National University, Busan, Korea.
Yun
Mi-ran
MR
Wang
Wei-hong
WH
Jung
Jee H
JH
Im
Dong-soon
DS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D001517:Coleoptera / Q000737:chemistry; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry; D013329:Structure-Activity Relationship
D008244:Lysophosphatidylcholines; D002118:Calcium