[Molecular and biological characterization of the CIBMUQ/HDC strain, a reference strain for Colombian Toxoplasma gondii].
There are few reports about characterization strains of Toxoplasma gondii that analyze the differences between isolates from Europe or United States with those obtained in South America. The current study analyzes virulence data from the mouse model, the gene SAG2 polymorphism by PCR-RFLP and microsatellite analysis in a single Colombian isolate. The strain was isolated from blood of a child with congenital toxoplasmosis, living in Armenia, Colombia. Analysis of virulence in the mouse showed that this strain has an LD100 of 10 tachyzoites. Both methods of genetic characterization demonstrated that this strain belonged to the clonal type 1 and was called HOM/CTCO/2002/CIBMUQ/BL/HDC (brief name: CIBMUQ/HDC). The CIBMUQ/HDC strain is the first Colombian strain available as a reference strain for national and international researchers.
Gallego
Carolina
C
Grupo de Estudio en Parasitologia Molecular, Centro de Investigaciones Biomédicas, Universidad del Quindio, Armenia, Colombia. gepamol@uniquindio.edu.co
Castaño
Jhon Carlos
JC
Giraldo
Alejandra
A
Ajzenberg
Daniel
D
Dardé
Marie-Laure
ML
Gómez
Jorge Enrique
JE
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis*; D003105:Colombia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis*; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008967:Molecular Biology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D014125:Toxoplasmosis, Congenital / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D037521:Virulence Factors