Immunohistochemical study of osteopontin in the spinal cords of rats with clip compression injury.
Expression of osteopontin (OPN) was investigated in the spinal cords of rats with clip compression injury. Western blot analysis demonstrated that OPN protein increased significantly in the spinal cord during the early stages after injury. The increased expression of OPN was partially paralleled by that of proliferating cell nuclear antigen (PCNA). Immunohistochemical staining showed that OPN was expressed in proliferating activated microglia/macrophages in core lesions and in some astrocytes at the periphery of lesions. These results indicate that expression of OPN protein increases mainly in activated microglia/macrophages after spinal cord injury, suggesting that OPN is related to cell proliferation during the early stages after injury, probably leading to tissue remodeling.
Moon
Changjong
C
Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University, Jeju, South Korea.
Heo
Seungdam
S
Ahn
Meejung
M
Kim
Heechul
H
Shin
Myungseung
M
Sim
Ki Bum
KB
Kim
Hyung Min
HM
Shin
Taekyun
T
D000818:Animals; D015153:Blotting, Western; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D053495:Osteopontin; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology
D018809:Proliferating Cell Nuclear Antigen; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin