Metabolism of the microregions of human breast cancer.
Glucose and glutamine metabolism of two microregions of human infiltrating ductile breast cancer, the center and the periphery, was studied and the results were compared with those of healthy mammary glands. In general, the activities of glycolytic enzymes and of phosphate-dependent glutaminase were as follows: center>periphery>mammary gland. Insulin caused a marked increase of glucose consumption and lactate production by incubated slices of mammary gland but had no effect on both microregions of the tumor. Therefore, human breast cancer presents metabolic microregions.
dos Santos
Maria Alves
MA
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900, Butantan, São Paulo, SP, Brazil.
Borges
João Bosco Ramos
JB
de Almeida
Débora Cristina Gonçalves
DC
Curi
Rui
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D019344:Lactic Acid / Q000096:biosynthesis; D008875:Middle Aged; D011770:Pyruvate Kinase / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
D007328:Insulin; D019344:Lactic Acid; D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D005634:Fructose-Bisphosphate Aldolase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose