Application of difference NOE-pumping NMR technique and cold-spray ionization mass spectrometry to identify a ligand binding with a protein receptor.
A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.
Seki
Hiroko
H
Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba 263-8522, Japan.
Sei
Yoshihisa
Y
Shikii
Kazuaki
K
Shimotakahara
Sakurako
S
Utsumi
Hiroaki
H
Yamaguchi
Kentaro
K
Tashiro
Mitsuru
M
D005665:Furosemide / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D020156:Salicylic Acid / Q000032:analysis / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature; D014859:Warfarin / Q000032:analysis / Q000737:chemistry
D008024:Ligands; D012709:Serum Albumin; D014859:Warfarin; D005665:Furosemide; D020156:Salicylic Acid