title: Profiling of methamphetamine-induced modifications of gene expression patterns in the mouse brain.
abstract: Recently described DNA microarray technology allows parallel screening of expression patterns and regulation of hundreds of thousands of genes. In the present study, we used a microarray to examine the gene expressions in the midbrains of mice sacrificed 24 h after completion of a 7-day treatment period consisting of a once-daily treatment with saline (SS), saline followed by a single 2 mg/kg of body weight dose of methamphetamine (METH) (S-METH), or repeated 2 mg/kg METH doses (M-METH) that produced sensitization and place preference (rewarding effect). We used the commercially available cDNA microarray. Approximately 80% of the assessed transcripts in the total brain reached the Affymetrix criteria for "present" and "changed," as well as displaying > or =1.5-fold differences in hybridization intensity difference values in a comparison of SS data to S-METH or M-METH data. S-METH gene expression changes were observed in both up- and down-regulation, with 13 transcripts upregulated and 13 downregulated, whereas the majority of M-METH gene expression changes were observed in down-regulation, with 5 transcripts upregulated and 21 downregulated. We identified several genes that altered expression in both the S-METH and M-METH groups: a transcription factor gene, cellular stress/molecular chaperones, and a cellular regulatory gene.
authors.affiliation: Division of Drug Dependence Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, 1-7-3 Kohnodai, Ichikawa, Chiba 272-0827, Japan. mfunada@ncnp-k.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Funada
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: K
authors.initials: K
authors.lastname: Wada
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects* / Q000235:genetics*; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics
chemical_list: D008694:Methamphetamine