Neuroprotection by encapsulated choroid plexus in a rodent model of Huntington's disease.
Choroid plexus from neonatal pigs was encapsulated in alginate microcapsules and transplanted into the rat striatum. Three days later, the same animals received unilateral injections of quinolinic acid (225 nmol) into the ipsilateral striatum. Choroid plexus transplants ameliorated the weight loss and motor impairments resulting from QA. Histological analysis demonstrated that choroid plexus transplants reduced the volume of striatal damage and protected ChAT-, but not NADPH-diaphorase-positive neurons. These data are the first to demonstrate that transplanted choroid plexus cells can protect striatal neurons from excitotoxic damage and that this strategy may ultimately prove relevant for the treatment of Huntington's disease.
Borlongan
Cesario V
CV
Neurology Department, Institute of Mol Med and Genetics and School of Graduate Studies, Medical College, 1120 15th Street, Augusta, GA 30912-3200, USA. cborlongan@mail.mcg.edu
Skinner
Steve J M
SJ
Geaney
Marilyn
M
Vasconcellos
Alfred V
AV
Elliott
Robert B
RB
Emerich
Dwaine F
DF
D000464:Alginates / Q000627:therapeutic use*; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D001835:Body Weight / Q000187:drug effects; D016380:Brain Tissue Transplantation / Q000379:methods; D002452:Cell Count / Q000379:methods; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002831:Choroid Plexus / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology / Q000637:transplantation; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D007839:Functional Laterality / Q000502:physiology; D020723:Glucuronic Acid / Q000627:therapeutic use*; D006603:Hexuronic Acids / Q000627:therapeutic use*; D006816:Huntington Disease / Q000139:chemically induced / Q000517:prevention & control*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009410:Nerve Degeneration / Q000517:prevention & control; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D017378:Quinolinic Acid / Q000633:toxicity; D051381:Rats; D013552:Swine; D013997:Time Factors
D000464:Alginates; D006603:Hexuronic Acids; D018696:Neuroprotective Agents; D020723:Glucuronic Acid; D002795:Choline O-Acetyltransferase; D017378:Quinolinic Acid