Attempts at correlation of the radiolytic species of irradiated solid-state captopril studied by multi-frequency EPR and HPLC.
The purpose of this study was to provide insight into the processes that occur after the irradiation of solid-state drugs. Electron paramagnetic resonance (EPR) experiments were performed at two different frequencies, X-band (about 9.5 GHz) and Q-band (about 34 GHz), to identify the radicals present in irradiated captopril. The results confirmed that an irradiated drug can trap several main radicals. Moreover, the radical composition varied as a function of the treatment. In addition, non-volatile final products were studied by liquid chromatography coupled to UV and to mass spectrometry (LC-MS). The variation of the radical composition did not influence the profile of the final products; this appears to indicate that, in the case of captopril, the trapped radicals observed by EPR are not the main precursors of the final products. Finally, high-performance liquid chromatography data appear to indicate that radiosterilization of captopril is feasible.
Engalytcheff
Alix
A
Laboratory of Chemical and Physicochemical Analysis of Drugs, UCL, B.1200 Brussels, Belgium. alix.engalytcheff@cham.ucl.ac.be
Debuyst
René
R
Vanhaelewyn
Gauthier C A M
GC
Callens
Freddy J
FJ
Tilquin
Bernard
B
D002216:Captopril / Q000737:chemistry / Q000528:radiation effects*; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D013058:Mass Spectrometry; D014961:X-Ray Diffraction
D002216:Captopril