Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM.
The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but non-invasive attachment of the cells. Using tapping-mode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. Significant differences in the morphology of RBCs from healthy humans and patients with systemic lupus erythematosus (SLE) were observed on topographical images. The surface of RBCs from SLE patients showed characteristic circular-shaped holes with approx. 200 nm in diameter under physiological conditions, a possible morphological correlate to previously published changes in the SLE erythrocyte membrane.
Kamruzzahan
A S M
AS
Institute of Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria.
Kienberger
Ferry
F
Stroh
Cordula M
CM
Berg
Jörg
J
Huss
Ralf
R
Ebner
Andreas
A
Zhu
Rong
R
Rankl
Christian
C
Gruber
Hermann J
HJ
Hinterdorfer
Peter
P
D004910:Erythrocyte Membrane / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism / Q000473:pathology; D018625:Microscopy, Atomic Force / Q000379:methods*; D013499:Surface Properties