In vitro screening for antiplasmodial activity of isoquinoline alkaloids from Brazilian plant species.
In the search for new antimalarial agents, nine Brazilian plant species were selected, from the Annonaceae (6), Menispermaceae (2) and Siparunaceae (1) families naturally occurring at the cerrado and Atlantic rainforest regions, in order to investigate their in vitro antiplasmodial activity. The ethanol and the alkaloid extracts were tested against K1, chloroquine-resistant, and Palo Alto, chloroquine-sensitive, strains of Plasmodium falciparum. The majority of the alkaloid extracts were more active than the ethanol ones, with IC(50) ranging 0.3-8.2 microg/mL. The crude Guatteria australis alkaloids were the most active against K1 with an IC(50) = 0.3 microg/mL. The most promising total alkaloid fractions for further bioguided isolation are those with the IC(50) < or = 5 microg/mL: G. australis, Cissampelos ovalifolia and Duguetia lanceolata.
Fischer
Dominique Corinne Hermine
DC
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Bloco15, 05580-900, São Paulo, SP, Brazil. domi@usp.br
de Amorim Gualda
Nilton Carlos
NC
Bachiega
Danielle
D
Carvalho
Cyntia Salerno
CS
Lupo
Fernanda Nogueira
FN
Bonotto
Sonia Valéria
SV
Alves
Mariana de Oliveira
Mde O
Yogi
Alvaro
A
Santi
Silvia Maria Di
SM
Avila
Priscilla Elisangela
PE
Kirchgatter
Karin
K
Moreno
Paulo Roberto Hrihorowitsch
PR
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001938:Brazil; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*
D000470:Alkaloids; D000962:Antimalarials; D007546:Isoquinolines; D010936:Plant Extracts