Modeling the Trypanosoma cruzi Tc85-11 protein and mapping the laminin-binding site.
Trypanosoma cruzi expresses a set of glycoproteins encoded by the gp85/trans-sialidase gene superfamily. In this report a structure model is proposed for a cloned member of the superfamily, the Tc85-11 protein. The structure consists of an N-terminus beta-propeller and a C-terminus beta-sandwich interconnected by an alpha-helix. The recombinant protein, corresponding to the N-domain (Tc85-N), binds to laminin in a selective manner. Six synthetic 20-mer peptides from the N-domain adhere onto the surface of LLC-MK(2) cells and two of these peptides specifically inhibit the Tc85-N/laminin interaction, indicating that they are the laminin-binding sites of the molecule. Thus, Tc85-11 and other related members of the family appear to be good candidates to play an important role in T. cruzi infection via a laminin mediated host-parasite interaction.
Marroquin-Quelopana
Miryam
M
Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, São Paulo, SP, Brazil.
Oyama
Sergio
S
Aguiar Pertinhez
Thelma
T
Spisni
Alberto
A
Aparecida Juliano
Maria
M
Juliano
Luiz
L
Colli
Walter
W
Alves
Maria Júlia M
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism*; D008253:Macaca mulatta; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism*
D006023:Glycoproteins; D007797:Laminin; D010455:Peptides; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C069715:trans-sialidase; D009439:Neuraminidase