title: Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells.
abstract: The improvement of techniques for in vitro expansion of cord blood (CB) hematopoietic stem cells (SCs) is, at present, one main task of tissue engineering. Hence, we investigated whether endothelin-1 (ET-1) and adrenomedullin (AM), two regulatory peptides exerting growth promoting action on several cell systems, favor the in vitro expansion of CB SCs in liquid culture. CB hematopoietic cell middle-term expansion was carried out in a stroma-free liquid culture medium in the presence of ET-1, AM and three different cytokine combinations. After two weeks of incubation, aliquots of expanded-cell suspension were seeded on semisolid medium and clonogenic tests were carried out by counting the number of colony forming units (CFUs) after 14 days of culture. Neither ET-1 nor AM (2.5 x 10(-8) M) were per se able to significantly increase the CFU number, but both peptides magnified the pro-expansive effects of some cytokine cocktails. In light of these findings, we conclude that ET-1 and AM are to be considered novel promising molecules that, in association with cytokines, can be utilized as pro-expansive factors of CB SCs in prevision of their clinical use in allogeneic transplantation.
authors.affiliation: Cell Culture Laboratory, Transfusional Center, Treviso Regional Hospital, I-31100 Treviso, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: De Angeli
authors.forename: Laura
authors.initials: L
authors.lastname: Del Pup
authors.forename: Erica
authors.initials: E
authors.lastname: Febas
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Mara
authors.initials: M
authors.lastname: Tommasini
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Liddo
authors.forename: Giovanna
authors.initials: G
authors.lastname: Albertin
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
authors.forename: Gastone G
authors.initials: GG
authors.lastname: Nussdorfer
mesh_terms: D053607:Adrenomedullin; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D019332:Endothelin-1 / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D010455:Peptides / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D019332:Endothelin-1; D010455:Peptides; D053607:Adrenomedullin