title: Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid complex.
abstract: Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid [Cu(ssal)(2)(2+)] complex was studied. The cleavage was observed in a certain potential region where redox cycling of Cu(ssal)(2)(2+)/Cu(ssal)(2)(+) took place. Cu(ssal)(2)(2+) complex mediate generation of reactive oxygen species from O(2) by the Fenton reaction, these radicals are capable of damaging DNA. The cleaved DNA fragments were separated by high-performance liquid chromatography (HPLC). The experimental results indicated that the method for electrochemical cleavage of DNA by Cu(ssal)(2)(2+) complex was simple and efficient.
authors.affiliation: School of Chemistry and Materials Science, Anhui Normal University, Wuhu 24100, PR China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Yun-Chun
authors.initials: YC
authors.lastname: Liu
authors.forename: Zhou-Sheng
authors.initials: ZS
authors.lastname: Yang
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Zhao
mesh_terms: D001557:Benzenesulfonates; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*
chemical_list: D001557:Benzenesulfonates; D017382:Reactive Oxygen Species; D012459:Salicylates; D003300:Copper; D004247:DNA; C003366:sulfosalicylic acid