title: Production of L-asparaginase by filamentous fungi.
abstract: L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of glutamine and urea as nitrogen sources. These results suggest that L-asparaginase production by of filamentous fungi is under nitrogen regulation.
authors.affiliation: Laboratório de Coleção de Fungos, Departamento de Micologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. isarquis@ioc.fiocruz.br
authors.forename: Maria Inez de Moura
authors.initials: MI
authors.lastname: Sarquis
authors.forename: Edna Maria Morais
authors.initials: EM
authors.lastname: Oliveira
authors.forename: Alberdan Silva
authors.initials: AS
authors.lastname: Santos
authors.forename: Gisela Lara da
authors.initials: GL
authors.lastname: Costa
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis*; D001230:Aspergillus / Q000201:enzymology*; D003470:Culture Media; D005670:Fusarium / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology*
chemical_list: D003470:Culture Media; D001215:Asparaginase; D009584:Nitrogen