title: Porcine small intestine submucosa does not show antimicrobial properties.
abstract: The goal of this study is to examine whether porcine small intestine submucosa (SIS) exhibits antimicrobial properties in a standard in vitro system, without pretreatment with acetic acid or extraction of soluble proteins. Previous animal studies suggest that porcine SIS may have inherent antibiotic properties. Using the guidelines for disk diffusion susceptibility testing by Bauer, 17/64-inch diameter disks made of porcine small intestine submucosa and of gortex were compared with standard antibiotic-impregnated disks against six organisms. The zone of inhibition was measured after 24 hours and minimum bacterial concentrations were determined by serial dilutions of a solution in which porcine small intestine submucosa was allowed to elute for 24 hours. Neither porcine SIS or gortex discs caused inhibition of the growth of any organism. The porcine small intestine submucosa discs showed bacterial growth on top of the discs whereas the gortex did not. Neither the dilutional concentrations of the porcine small intestine submucosa eluent nor the gortex eluent inhibited the growth of any organism. These findings suggest that the porcine small intestine submucosa does not have intrinsic antimicrobial properties. The growth of bacteria on top of the porcine small intestine submucosa suggests that porcine small intestine submucosa itself may provide a favorable environment for the growth of bacteria. More research is necessary to decide what role porcine small intestine submucosa plays in the treatment of infected surgical sites.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of Southern California, Keck School of Medicine Los Angeles, CA, USA. Holtom@usc.edu
authors.forename: Paul D
authors.initials: PD
authors.lastname: Holtom
authors.forename: Zachary
authors.initials: Z
authors.lastname: Shinar
authors.forename: Jennifer
authors.initials: J
authors.lastname: Benna
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Patzakis
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000502:physiology*; D007421:Intestine, Small / Q000382:microbiology* / Q000502:physiology*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents