Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo.
One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds.
Tan
Lay-Pheng
LP
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Lue
Rina Y P
RY
Chen
Grace Y J
GY
Yao
Shao Q
SQ
D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D019921:Biotinylation; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D047668:Inteins / Q000502:physiology; D040081:Protein Array Analysis / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D011506:Proteins; D049452:Green Fluorescent Proteins; D001710:Biotin