title: Loss of endothelium-dependent relaxation in abdominal aorta preserved in a co-storage system.
abstract: The potentially detrimental influence of parenchymal cells on endothelial function during preservation in UW solution was examined by co-storage of rat abdominal aortic rings with isolated liver cells. Cold storage of rings in UW solution alone for up to 96 h had no effect on the response to acetylcholine, though constriction was progressively lost. Co-storage of rings with liver cells resulted in no loss of sodium nitroprusside response, but the relaxation response to acetylcholine was reduced. The loss of acetylcholine response could not be attributed to Kupffer cells, the lowering of pH, oxygen depletion, or the loss of constriction. A similar loss of endothelial function was observed in rings stored in pieces of liver, kidney or heart. We conclude that parenchymal cells exude factors during preservation by cold storage which reversibly inhibit vascular NO production. These factors could significantly impair whole organ function on reperfusion.
authors.affiliation: Division of Transplantation Department of Surgery, University of Wisconsin Clinical Science Center, 600 Highland Avenue, Madison, WI 53792, USA.
authors.forename: Jane M
authors.initials: JM
authors.lastname: Knes
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Hansen
authors.forename: Barbara
authors.initials: B
authors.lastname: Gilligan
authors.forename: Heung
authors.initials: H
authors.lastname: Woo
authors.forename: Martin
authors.initials: M
authors.lastname: Mangino
authors.forename: Robert A
authors.initials: RA
authors.lastname: Haworth
authors.forename: James H
authors.initials: JH
authors.lastname: Southard
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D015925:Cryopreservation; D004357:Drug Synergism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology*; D005978:Glutathione / Q000494:pharmacology; D022781:Hepatocytes; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007728:Kupffer Cells; D008099:Liver / Q000503:physiopathology; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D009926:Organ Preservation / Q000009:adverse effects* / Q000379:methods; D019188:Organ Preservation Solutions / Q000494:pharmacology; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014664:Vasodilation; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014665:Vasodilator Agents; D009599:Nitroprusside; D000493:Allopurinol; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D000109:Acetylcholine