DNA instructed displacement of histones H2A and H2B at an inducible promoter.
Regulation of gene expression requires dynamic changes in chromatin, but the nature of these changes is not well understood. Here, we show that progesterone treatment of cultured cells leads to recruitment of progesterone receptor (PR) and SWI/SNF-related complexes to Mouse Mammary Tumor Virus (MMTV) promoter, accompanied by displacement of histones H2A and H2B from the nucleosome containing the receptor binding sites, but not from adjacent nucleosomes. PR recruits SWI/SNF to MMTV nucleosomes in vitro and facilitates synergistic binding of receptors and nuclear factor 1 to the promoter. In nucleosomes assembled on MMTV or mouse rDNA promoter sequences, SWI/SNF catalyzes ATP-dependent sliding of the histone octamer followed only on the MMTV promoter by displacement of histones H2A and H2B. In MMTV nucleosome arrays, SWI/SNF displaces H2A and H2B from nucleosome B and not from the adjacent nucleosome. Thus, the outcome of nucleosome remodeling by SWI/SNF depends on DNA sequence.
Vicent
Guillermo P
GP
Centre de Regulació Genòmica, Universitat Pompeu Fabra, Passeig Marítim 37-49, E-08003 Barcelona, Spain.
Nacht
A Silvina
AS
Smith
Corey L
CL
Peterson
Craig L
CL
Dimitrov
Stefan
S
Beato
Miguel
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011980:Receptors, Progesterone / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D002868:Chromosomal Proteins, Non-Histone; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D006657:Histones; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009707:Nucleosomes; D011980:Receptors, Progesterone; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; C080333:transcription factor nuclear factor 1; D011374:Progesterone; D000255:Adenosine Triphosphate