title: ApTrkl, a Trk-like receptor, mediates serotonin- dependent ERK activation and long-term facilitation in Aplysia sensory neurons.
abstract: The Trk family of receptor tyrosine kinases plays a role in synaptic plasticity and in behavioral memory in mammals. Here, we report the discovery of a Trk-like receptor, ApTrkl, in Aplysia. We show that it is expressed in the sensory neurons, the locus for synaptic facilitation, which is a cellular model for memory formation. Serotonin, the facilitatory neurotransmitter, activates ApTrkl, which, in turn, leads to activation of ERK. Finally, inhibiting the activation of ApTrkl with the Trk inhibitor K252a or using dsRNA to inhibit ApTrkl blocks the serotonin-mediated activation of ERK in the cell body, as well as the cell-wide long-term facilitation induced by 5-HT application to the cell body. Thus, transactivation of the receptor tyrosine kinase ApTrkl by serotonin is an essential step in the biochemical events leading to long-term facilitation in Aplysia.
authors.affiliation: Montreal Neurological Institute, McGill University, 3801 University Avenue, Montreal, Quebec H3A-2B4, Canada.
authors.forename: Jake
authors.initials: J
authors.lastname: Ormond
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hislop
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhao
authors.forename: Neil
authors.initials: N
authors.lastname: Webb
authors.forename: Francois
authors.initials: F
authors.lastname: Vaillaincourt
authors.forename: John R
authors.initials: JR
authors.lastname: Dyer
authors.forename: Gino
authors.initials: G
authors.lastname: Ferraro
authors.forename: Phil
authors.initials: P
authors.lastname: Barker
authors.forename: Kelsey C
authors.initials: KC
authors.lastname: Martin
authors.forename: Wayne S
authors.initials: WS
authors.lastname: Sossin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000502:physiology*; D001483:Base Sequence; D015153:Blotting, Western; D004789:Enzyme Activation / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017774:Long-Term Potentiation / Q000502:physiology; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D012701:Serotonin; D020794:Receptor Protein-Tyrosine Kinases; D048049:Extracellular Signal-Regulated MAP Kinases