[Molecular analysis of methicillin-resistant staphylococci isolated from hospital patients and in the out-patient clinic].
In this study, a molecular analysis of the methicillin-resistant coagulase-negative staphylococci strains was performed. The obtained results of the biochemical and drug resistant pattern investigations were insufficient to assess the relationship between the strains. Therefore genotyping by the restriction fragment length polymorphism analysis (PCR-RFLP) method was performed. Analyzed strains characterized presence of the mecA gene-PCR products. The PCR products were digested with DraI and TasI, and the fragments separated by agarose gel electrophoresis. Typing of the methicillin-resistant gene using PCR-RFLP showed that all MRCNS strains possess an identical restriction pattern of the mecA gene. This identical restriction pattern of the mecA gene in investigated strains may suggest an easy transfer of this gene between different staphylococci species and lead to the spreading of methicillin-resistant among hospital strains. Furthermore performing the comparison of different phenotype and genotype methods has shown that the PCR-RFLP method is quick and reliable, enabling the detection and estimation of the relationship between MRCNS strains.
Małecka-Mikosz
Oliwia
O
Zakład Ogólnej Biologii Lekarskiej Slaskiej, Akademii Medycznej w Katowicach.
Wiczkowski
Andrzej
A
Strzelczyk
Joanna
J
Zalewska-Ziob
Marzena
M
Dyla
Lucja
L
Kwaśniewski
Mirosław
M
D001426:Bacterial Proteins / Q000378:metabolism; D006801:Humans; D007297:Inpatients; D016106:Methicillin Resistance / Q000235:genetics*; D010045:Outpatients; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; C487495:MecA protein, Staphylococcus epidermidis; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus