Localization of zinc transporter-3 (ZnT-3) in mouse retina.
Studies of the central nervous system have localized the zinc-transporter-3 (ZnT-3) protein to synaptic vesicles containing glutamate and zinc. We have examined the distribution of the ZnT-3 protein in the light-adapted mouse retina using immunohistochemical techniques. Light microscopic analysis of 15-30-microm retinal sections revealed a rich band of ZnT-3 protein in the region of the outer limiting membrane and photoreceptor inner segments. ZnT-3 reactivity was also present in the outer plexiform, inner nuclear, inner plexiform, and ganglion cell layers. The outer nuclear layer and photoreceptor outer segments did not exhibit ZnT-3 immunoreactivity. In the light-adapted murine retina, ZnT-3 appears localized in regions which have been found reactive for ionic zinc.
Redenti
Stephen
S
Ph.D. Program in Biology, The Graduate Center, CUNY, 365 Fifth Avenue, New York, NY, USA.
Chappell
Richard L
RL
D000221:Adaptation, Ocular / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D027682:Cation Transport Proteins; D007124:Immunoenzyme Techniques; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D026901:Membrane Transport Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D012160:Retina / Q000737:chemistry*; D015032:Zinc / Q000378:metabolism
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C491177:Slc30a3 protein, mouse; D015032:Zinc