Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification.
We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis.
Horisaka
Tomoko
T
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Fujita
Kayoko
K
Iwata
Taketoshi
T
Nakadai
Aya
A
Okatani
Alexandre T
AT
Horikita
Tetsuya
T
Taniguchi
Takahide
T
Honda
Eiichi
E
Yokomizo
Yuichi
Y
Hayashidani
Hideki
H
D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D018420:Ape Diseases / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D008992:Monkey Diseases / Q000175:diagnosis / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D011148:Pongo pygmaeus; D012453:Saimiri; D012680:Sensitivity and Specificity; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis / Q000662:veterinary*
D018829:Adhesins, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial