title: Detection and selection of microsatellites in the genome of Paracoccidioides brasiliensis as molecular markers for clinical and epidemiological studies.
abstract: Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.
authors.affiliation: Departamento de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: Erika
authors.initials: E
authors.lastname: Nascimento
authors.forename: Roberto
authors.initials: R
authors.lastname: Martinez
authors.forename: André Rodrigues
authors.initials: AR
authors.lastname: Lopes
authors.forename: Luciano Angelo
authors.initials: LA
authors.lastname: de Souza Bernardes
authors.forename: Carolina Pomponio
authors.initials: CP
authors.lastname: Barco
authors.forename: Maria Helena S
authors.initials: MH
authors.lastname: Goldman
authors.forename: John W
authors.initials: JW
authors.lastname: Taylor
authors.forename: Juan G
authors.initials: JG
authors.lastname: McEwen
authors.forename: Marina Pasetto
authors.initials: MP
authors.lastname: Nobrega
authors.forename: Francisco G
authors.initials: FG
authors.lastname: Nobrega
authors.forename: Gustavo H
authors.initials: GH
authors.lastname: Goldman
mesh_terms: D000818:Animals; D001135:Armadillos / Q000382:microbiology; D005819:Genetic Markers; D016681:Genome, Fungal; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014774:Virulence
chemical_list: D005819:Genetic Markers