title: Modeling the Trypanosoma cruzi Tc85-11 protein and mapping the laminin-binding site.
abstract: Trypanosoma cruzi expresses a set of glycoproteins encoded by the gp85/trans-sialidase gene superfamily. In this report a structure model is proposed for a cloned member of the superfamily, the Tc85-11 protein. The structure consists of an N-terminus beta-propeller and a C-terminus beta-sandwich interconnected by an alpha-helix. The recombinant protein, corresponding to the N-domain (Tc85-N), binds to laminin in a selective manner. Six synthetic 20-mer peptides from the N-domain adhere onto the surface of LLC-MK(2) cells and two of these peptides specifically inhibit the Tc85-N/laminin interaction, indicating that they are the laminin-binding sites of the molecule. Thus, Tc85-11 and other related members of the family appear to be good candidates to play an important role in T. cruzi infection via a laminin mediated host-parasite interaction.
authors.affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, São Paulo, SP, Brazil.
authors.forename: Miryam
authors.initials: M
authors.lastname: Marroquin-Quelopana
authors.forename: Sergio
authors.initials: S
authors.lastname: Oyama
authors.forename: Thelma
authors.initials: T
authors.lastname: Aguiar Pertinhez
authors.forename: Alberto
authors.initials: A
authors.lastname: Spisni
authors.forename: Maria
authors.initials: M
authors.lastname: Aparecida Juliano
authors.forename: Luiz
authors.initials: L
authors.lastname: Juliano
authors.forename: Walter
authors.initials: W
authors.lastname: Colli
authors.forename: Maria Júlia M
authors.initials: MJ
authors.lastname: Alves
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism*; D008253:Macaca mulatta; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D007797:Laminin; D010455:Peptides; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C069715:trans-sialidase; D009439:Neuraminidase