Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC.
The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan.
Li
Famei
F
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. fameili@hotmail.com
Zhang
Dandan
D
Lu
Xiumei
X
Wang
Yuling
Y
Xiong
Zhili
Z
D001881:Borates; D002021:Buffers; D002316:Cardiotonic Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006835:Hydrazones / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011724:Pyridazines / Q000032:analysis* / Q000737:chemistry; D000077464:Simendan; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D001881:Borates; D002021:Buffers; D002316:Cardiotonic Agents; D006835:Hydrazones; D007202:Indicators and Reagents; D011724:Pyridazines; D047392:beta-Cyclodextrins; D000077464:Simendan; C031215:betadex