title: Expression of a vaccine protein in a plant cell line using a geminivirus-based replicon system.
abstract: Edible vaccines have been generated from both transgenic plants as well as from plant viral vectors. Here, we have taken the best attributions of both systems and designed a minimalized version of the bean yellow dwarf geminivirus (BeYDV)-based replicon consisting of the cis-acting elements required for BeYDV replication as a means to express foreign genes in a plant cell line. Replication can be switched on at high levels upon expression of the BeYDV Rep protein, and gene expression enhanced enormously. Construction of an expression cassette encoding a synthetic vaccine gene and analysis of expression levels of a vaccine protein in a plant cell line system are described.
authors.affiliation: Cornell Research Foundation, Cornell University, 20 Thornwood Dr., Ithaca, NY 14850, USA. klh22@cornell.edu
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Hefferon
authors.forename: Ying
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D015139:Blotting, Southern; D002460:Cell Line; D002478:Cells, Cultured; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D017791:Geminiviridae / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D030821:Plants, Genetically Modified / Q000166:cytology / Q000378:metabolism; D012093:Replicon; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D022462:Vaccines, Edible / Q000235:genetics; D014779:Virus Replication
chemical_list: D000942:Antigens, Bacterial; D004768:Enterotoxins; D022462:Vaccines, Edible; D049452:Green Fluorescent Proteins; C031818:enterotoxin B, staphylococcal