title: Effects of ornithine on neutrophil (PMN) free amino acid and alpha-keto acid profiles and immune functions in vitro.
abstract: The objective of this study was to determine the effects of ornithine on polymorphonuclear leucocyte (PMN) free amino- and alpha-keto acid profiles, superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase activity (MPO). Exogenous ornithine significantly increased PMN asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, alpha-ketoglutarate and pyruvate as intracellular ornithine increased. Concerning PMN immune function markers ornithine increased H2O2-generation and MPO activity while O2- -formation was decreased. We believe therefore that ornithine is important for affecting PMN "susceptible free amino- and alpha-keto acid pool" although the mechanisms are not yet clear. This may be one of the determinants in PMN nutrition considerably influencing and modulating PMN host defense capability.
authors.affiliation: Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany. joerg.muehling@chiru.med.uni-giessen.de
authors.forename: J
authors.initials: J
authors.lastname: Mühling
authors.forename: M
authors.initials: M
authors.lastname: Fuchs
authors.forename: M
authors.initials: M
authors.lastname: Campos
authors.forename: J
authors.initials: J
authors.lastname: Gonter
authors.forename: A
authors.initials: A
authors.lastname: Sablotzki
authors.forename: J
authors.initials: J
authors.lastname: Engel
authors.forename: I D
authors.initials: ID
authors.lastname: Welters
authors.forename: M
authors.initials: M
authors.lastname: Wolff
authors.forename: R
authors.initials: R
authors.lastname: Matejec
authors.forename: M G
authors.initials: MG
authors.lastname: Dehne
authors.forename: T
authors.initials: T
authors.lastname: Menges
authors.forename: M
authors.initials: M
authors.lastname: Krüll
authors.forename: G
authors.initials: G
authors.lastname: Hempelmann
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000097:blood*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D007651:Keto Acids / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D009952:Ornithine / Q000097:blood / Q000494:pharmacology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000097:blood / Q000187:drug effects; D013481:Superoxides / Q000097:blood
chemical_list: D000596:Amino Acids; D007651:Keto Acids; D013481:Superoxides; D006861:Hydrogen Peroxide; D009952:Ornithine; D009195:Peroxidase