Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China.
Ninety-five rhizobial strains isolated from Astragalus adsurgens growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis of full-length 16S rRNA gene sequences of representative strains showed that candidate species I and II were Mesorhizobium, while candidate species III, which consisted of non-nodulating strains, was closely related to Agrobacterium tumefaciens. The phylogenetic relationships of the three candidate species and some related strains were also confirmed by the sequencing of glnA genes, which were used as an alternative chromosomal marker. The DNA-DNA relatedness was between 11.3 and 47.1 % among representative strains of candidate species I and II and the type strains of defined Mesorhizobium species. Candidate III had DNA relatedness of between 4.3 and 25.2 % with type strains of Agrobacterium tumefaciens and Agrobacterium rubi. Two novel species are proposed to accommodate candidate species I and II, Mesorhizobium septentrionale sp. nov. (type strain, SDW014(T)=CCBAU 11014(T)=HAMBI 2582(T)) and Mesorhizobium temperatum sp. nov. (type strain, SDW018(T)=CCBAU 11018(T)=HAMBI 2583(T)), respectively. At least two distinct nodA sequences were identified among the strains. The numerically dominant nodA sequence type was most similar to that from the Mesorhizobium tianshanense type strain and was identified in strains belonging to the two novel species as well as other, as yet, undefined genome types. Host range studies indicate that the different nodA sequences correlate with different host ranges. Further comparative studies with the defined Agrobacterium species are needed to clarify the taxonomic identity of candidate species III.
Gao
Jun-Lian
JL
Department of Biology, University of York, PO Box 373, York Y01 5YW, UK.|Department of Applied Chemistry and Microbiology, Biocenter 1, PO Box 56, FIN-00014, University of Helsinki, Finland.|Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Turner
Sarah Lea
SL
Department of Biology, University of York, PO Box 373, York Y01 5YW, UK.
Kan
Feng Ling
FL
Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Wang
En Tao
ET
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D.F. 11340, Mexico.|Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Tan
Zhi Yuan
ZY
Department of Molecular Genetics, College of Agronomy, South China Agricultural University, Guangzhou 510642, China.
Qiu
Yu Hui
YH
Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Gu
Jun
J
Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Terefework
Zewdu
Z
Department of Applied Chemistry and Microbiology, Biocenter 1, PO Box 56, FIN-00014, University of Helsinki, Finland.
Young
J Peter W
JPW
Department of Biology, University of York, PO Box 373, York Y01 5YW, UK.
Lindström
Kristina
K
Department of Applied Chemistry and Microbiology, Biocenter 1, PO Box 56, FIN-00014, University of Helsinki, Finland.
Chen
Wen Xin
WX
Key Laboratory of Agro-Microbial Resources and Application, Ministry of Agriculture of China, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
D000217:Acyltransferases / Q000235:genetics; D020561:Alphaproteobacteria / Q000145:classification* / Q000302:isolation & purification*; D027883:Astragalus Plant / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D005838:Genotype; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase