Visualizing dendritic cell networks in vivo.
In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.
Lindquist
Randall L
RL
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.
Shakhar
Guy
G
Dudziak
Diana
D
Wardemann
Hedda
H
Eisenreich
Thomas
T
Dustin
Michael L
ML
Nussenzweig
Michel C
MC
D000818:Animals; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*
D039521:CD11c Antigen; D011994:Recombinant Proteins