Effects of short and long incubations on DNA fragmentation of testicular sperm.
DNA fragmentation in testicular sperm from men with obstructive azoospermia is increased by 4-hour and 24-hour incubations and after cryopreservation with the effect is intensified by post-thaw incubation. Testicular sperm for use in intracytoplasmic sperm injection (ICSI) should be injected without delay.
Dalzell
Lauren H
LH
McVicar
Carmel M
CM
McClure
Neil
N
Lutton
Deborah
D
Lewis
Sheena E M
SE
D018929:Cell Culture Techniques; D015925:Cryopreservation; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D013094:Spermatozoa; D013737:Testis; D013997:Time Factors