Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis.
The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed.
Shiraishi
Masahiko
M
DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan. mshirais@gan2.res.ncc.go.jp
Oates
Adam J
AJ
Li
Xu
X
Chuu
Ying H
YH
Sekiya
Takao
T
D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans