Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China.
A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14(T), was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable gamma-region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus Streptomyces. The sharp separation of the organism from representatives of the genus Streptomyces was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized Streptomyces species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14(T) (=AS 4.1858(T)=JCM 12278(T)=LMG 22082(T)) should be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces glauciniger sp. nov. is proposed.
Huang
Ying
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Li
Wei
W
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Liming
L
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Lanoot
Benjamin
B
BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Rodriguez
Carlos
C
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Liu
Zhiheng
Z
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
D015373:Bacterial Typing Techniques; D002681:China; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S