title: Catalytic signal amplification using a Heck reaction. An example in the fluorescence sensing of CuII.
abstract: Catalytic signal enhancement using an organometallic reaction is demonstrated. The reactivity of a Heck cross-coupling reaction that creates a fluorophore is modulated by the addition of a polyazacyclam inhibitor. The inhibitor will complex with Cu(II), which restores the activity of the Pd(II). The addition of Cu(II) therefore leads to the generation of fluorescence, thereby creating a very sensitive assay for Cu(II). The rate of the Heck reaction is followed by monitoring emission as a function of time. The rate is proportional to the Cu(II) concentration and correlates to the affinity of the inhibitor to various metals. This strategy represents a general technique that can be exploited with other catalytic organometallic reactions.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Qiaoyin
authors.initials: Q
authors.lastname: Wu
authors.forename: Eric V
authors.initials: EV
authors.lastname: Anslyn
mesh_terms: D001372:Aza Compounds / Q000737:chemistry; D002384:Catalysis; D002413:Cations, Divalent; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D003500:Cyclization; D005453:Fluorescence; D006571:Heterocyclic Compounds / Q000737:chemistry*; D007700:Kinetics; D008670:Metals / Q000032:analysis / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010777:Photochemistry; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
chemical_list: D001372:Aza Compounds; D002413:Cations, Divalent; D006571:Heterocyclic Compounds; D008670:Metals; D009942:Organometallic Compounds; C024560:cyclam; D010165:Palladium; D003300:Copper