Effects of ornithine on neutrophil (PMN) free amino acid and alpha-keto acid profiles and immune functions in vitro.
The objective of this study was to determine the effects of ornithine on polymorphonuclear leucocyte (PMN) free amino- and alpha-keto acid profiles, superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase activity (MPO). Exogenous ornithine significantly increased PMN asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, alpha-ketoglutarate and pyruvate as intracellular ornithine increased. Concerning PMN immune function markers ornithine increased H2O2-generation and MPO activity while O2- -formation was decreased. We believe therefore that ornithine is important for affecting PMN "susceptible free amino- and alpha-keto acid pool" although the mechanisms are not yet clear. This may be one of the determinants in PMN nutrition considerably influencing and modulating PMN host defense capability.
Mühling
J
J
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany. joerg.muehling@chiru.med.uni-giessen.de
Fuchs
M
M
Campos
M
M
Gonter
J
J
Sablotzki
A
A
Engel
J
J
Welters
I D
ID
Wolff
M
M
Matejec
R
R
Dehne
M G
MG
Menges
T
T
Krüll
M
M
Hempelmann
G
G
D000328:Adult; D000596:Amino Acids / Q000097:blood*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D007651:Keto Acids / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D009952:Ornithine / Q000097:blood / Q000494:pharmacology*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000097:blood / Q000187:drug effects; D013481:Superoxides / Q000097:blood
D000596:Amino Acids; D007651:Keto Acids; D013481:Superoxides; D006861:Hydrogen Peroxide; D009952:Ornithine; D009195:Peroxidase