title: [Molecular and biological characterization of the CIBMUQ/HDC strain, a reference strain for Colombian Toxoplasma gondii].
abstract: There are few reports about characterization strains of Toxoplasma gondii that analyze the differences between isolates from Europe or United States with those obtained in South America. The current study analyzes virulence data from the mouse model, the gene SAG2 polymorphism by PCR-RFLP and microsatellite analysis in a single Colombian isolate. The strain was isolated from blood of a child with congenital toxoplasmosis, living in Armenia, Colombia. Analysis of virulence in the mouse showed that this strain has an LD100 of 10 tachyzoites. Both methods of genetic characterization demonstrated that this strain belonged to the clonal type 1 and was called HOM/CTCO/2002/CIBMUQ/BL/HDC (brief name: CIBMUQ/HDC). The CIBMUQ/HDC strain is the first Colombian strain available as a reference strain for national and international researchers.
authors.affiliation: Grupo de Estudio en Parasitologia Molecular, Centro de Investigaciones Biomédicas, Universidad del Quindio, Armenia, Colombia. gepamol@uniquindio.edu.co
authors.forename: Carolina
authors.initials: C
authors.lastname: Gallego
authors.forename: Jhon Carlos
authors.initials: JC
authors.lastname: Castaño
authors.forename: Alejandra
authors.initials: A
authors.lastname: Giraldo
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Dardé
authors.forename: Jorge Enrique
authors.initials: JE
authors.lastname: Gómez
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis*; D003105:Colombia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis*; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008967:Molecular Biology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D014125:Toxoplasmosis, Congenital / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D037521:Virulence Factors