title: Psychrophilicity of Bacillus psychrosaccharolyticus: a proteomic study.
abstract: Psychrophilicity of Gram-positive bacterium, Bacillus psychrosaccharolyticus was investigated in a proteomic approach. One hundred and thirty-one protein spots were analyzed by electrospray ionization-quadrupole-time of flight-tandem mass spectrometry and identified using an unpublished translated contig database as well as a nonredundant Gram-positive bacteria protein database from NCBI because of the lack of a genome sequence of this organism. Results focused on proteomic behavior of cold-response show that global up-regulation of metabolic functions and protective mechanism by stress responses might play a major role in psychrophilicity of B. psychrosaccharolyticus.
authors.affiliation: Seoul Branch, Korea Basic Science Institute, Seoul.
authors.forename: Jong Bok
authors.initials: JB
authors.lastname: Seo
authors.forename: Hye Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Gyoo Yeol
authors.initials: GY
authors.lastname: Jung
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Nam
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Chung
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
authors.forename: Chan Wha
authors.initials: CW
authors.lastname: Kim
authors.forename: Ohoak
authors.initials: O
authors.lastname: Kwon
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003080:Cold Temperature; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000378:metabolism; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D020543:Proteome