[Effect of combination of Ginkgo leaf extract and deferoxamine in preventing and treating ototoxicity of cisplatin].
OBJECTIVE
To observe the effect of combined use of jinnaduo (an injection made by extract of Ginkgo leaf, EGb) and Deferoxamine (DFO, a chelating agent) in antagonizing the ototoxicity of cisplatin (CDDP).


METHODS
Guinea pigs were randomly divided into the CDDP group, the EGb group, the DFO group, the combined treated group (EGb + DFO) and the control group. Changes of auditory brain-stem response (ABR), serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, as well as light and scanning electronic microscopic (SEM) figures were observed before and after treatment.


RESULTS
The threshold of ABR was significantly higher in the CDDP group than that in the other groups (P<0.01), but was insignificantly different among the latter groups (P>0.05). Serum SOD activity was lower and MDA content was higher in the CDDP group than those in the control group (P<0.01), but in comparison of the two parameters between control and other groups, the difference was insignificant (P>0.05). SEM examination on cochlea showed that the damage of hair cells was milder in the DFO group and the combined treated group than that in the CDDP group, which was slightly milder in the EGb group than that in the CDDP group.


CONCLUSIONS
Combined use of EGb and DFO could effectively reduce the ototoxicity of CDDP, its effect is better than using EGb singly, and similar to that of using DFO alone. The combination could also prevent the side-effect of CDDP in bone marrow inhibition. The Fe ion participated free radical response could be one of the mechanisms of CDDP in damaging hearing.
Xu
Ou
O
Department of Otorhinolaryngology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000. xuou1978@sina.com
Lu
Hong
H
Li
Pei-qin
PQ
Zhang
Xiqin
X
Lu
Zhe
Z
D000818:Animals; D002945:Cisplatin / Q000009:adverse effects*; D003051:Cochlea / Q000187:drug effects; D003676:Deferoxamine / Q000494:pharmacology*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D020441:Ginkgo biloba / Q000737:chemistry*; D006168:Guinea Pigs; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood
D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D003676:Deferoxamine; D002945:Cisplatin