title: Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China.
abstract: A diarrhoeal outbreak among adults in China was caused by a new rotavirus, termed ADRV-N, that does not react with antisera directed against group A, B or C rotaviruses [Zhonghua Liu Xing Bing Xue Za Zhi (Chin. Epidemiol.) 19 (1998) 336]. ADRV-N can be propagated in cell cultures [Zhonghua Yi Xue Za Zhi (Natl. Med. J. China) 82 (2002) 14]. We present the complete sequences for ADRV-N genome segments 5 and 6, and a full ORF sequence of genome segment 7. The deduced amino acid sequences suggest that these segments encode NSP1, VP6 and NSP3, respectively. These three ADRV-N genome segments have a unique -ACCCC-3' terminal sequence. The 5'-GG- terminus of segments 5 and 6 is the same as that of other rotaviruses. The amino acid similarity between VP6 and NSP3 of ADRV-N and the cognate sequences of their closest counterpart, group B IDIR, was 37 and 35%, respectively. The ADRV-N NSP1 has a double-stranded RNA binding motif (DSRM) and a putative autoproteolytic cleavage motif upstream from the DSRM. The putative ADRV-N NSP3 has a truncated C-terminus compared to the cognate protein of group B rotaviruses. All the available data demonstrate that ADRV-N differs significantly from the known rotaviruses and strongly suggest that ADRV-N is the first recognized member of a new group of rotaviruses infecting humans.
authors.affiliation: Institute of Biotechnology and Faculty of Biosciences, University of Helsinki, Biocenter 2, P.O. Box 56 (Viikinkaari 5), FIN-00014 Helsinki, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: E V
authors.initials: EV
authors.lastname: Makeyev
authors.forename: Z
authors.initials: Z
authors.lastname: Kang
authors.forename: S
authors.initials: S
authors.lastname: Ji
authors.forename: D H
authors.initials: DH
authors.lastname: Bamford
authors.forename: A A
authors.initials: AA
authors.lastname: van Dijk
mesh_terms: D000328:Adult; D036022:Capsid Proteins / Q000235:genetics; D002681:China; D003001:Cloning, Molecular; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D017421:Sequence Analysis
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral