RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein.
Differential screening of a cDNA library constructed using poly(A)(+) RNA from suspension-cultured rice cells treated with jasmonic acid (JA) for 1/2h yielded a cDNA of a gene tentatively named RERJ1 that is upregulated in response to exogenous JA. Northern blot analysis indicated that the RERJ1 mRNA levels peaked at 1/2-1h after the addition of jasmonic acid and then decreased gradually. RERJ1 encodes a transcriptional regulator with a basic helix-loop-helix motif. The phenotypes of transgenic rice plants overexpressing sense or antisense RERJ1 mRNA demonstrated that RERJ1 is involved in the growth inhibition of rice shoots caused by JA. Other biological functions of RERJ1 are discussed from an evolutionary standpoint.
Kiribuchi
Kyoko
K
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Sugimori
Miho
M
Takeda
Masayoshi
M
Otani
Takashi
T
Okada
Kazunori
K
Onodera
Haruko
H
Ugaki
Masashi
M
Tanaka
Yuji
Y
Tomiyama-Akimoto
Chiharu
C
Yamaguchi
Takeshi
T
Minami
Eiichi
E
Shibuya
Naoto
N
Omori
Toshio
T
Nishiyama
Makoto
M
Nojiri
Hideaki
H
Yamane
Hisakazu
H
D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D018257:Helix-Loop-Helix Motifs / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000502:physiology*; D010940:Plant Proteins; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C495143:RERJ1 protein, rice