Monitoring D-serine dynamics in the rat brain using online microdialysis-capillary electrophoresis.
D-Serine was detected in dialysate collected from the rat striatum using an online microdialysis-CE-LIF instrument. Dialysate can be analyzed every 12.5 s using the online instrument, giving much better temporal resolution than previously possible for D-serine. Basal concentrations of D-serine (8 +/- 2 microM), glutamate (0.8 +/- 0.2 microM), GABA (0.11 +/- 0.04 microM), and L-serine (23 +/- 4 microM) were measured. Increases in the concentrations of these neurochemicals induced by the introduction of high-K+ aCSF were quantitated. Notably, an increase in D-serine concentration in response to high-K+ aCSF was observed for the first time. The identity of the D-serine peak was confirmed unambiguously using D-amino acid oxidase to selectively remove D-serine from a dialysate sample. The microdialysis-CE-LIF instrument was able to monitor this enzymatic reaction as it proceeded over a period of 60 min, demonstrating that online microdialysis-CE-LIF is not only useful in monitoring in vivo dynamics but can also be used to monitor other chemical systems.
Ciriacks
Chanda M
CM
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA.
Bowser
Michael T
MT
D000818:Animals; D001921:Brain / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D017551:Microdialysis; D051381:Rats; D012694:Serine / Q000378:metabolism*
D012694:Serine