Abnormal RNA processing and altered expression of serin-rich proteins in minimal-change nephrotic syndrome.
Mechanisms underlying the pathophysiology of minimal-change nephrotic syndrome (MCNS), the most frequent glomerular disease in children, remain elusive, but recent findings argue for a T cell dysfunction. Starting from a differential cDNA library from T cells of a patient under relapse and remission, we identified 16 transcripts specific for MCNS. All of these transcripts that were selectively up-regulated during the relapse phase of the disease were generated by alternative splicing of known genes. This abnormal RNA expression was associated with a down-regulation of serin-rich protein 75 and serin-rich protein 40, two proteins involved in mRNA splicing. Taken together, these data suggest that T cell dysfunction in MCNS is associated with abnormal mRNA splicing.
Grimbert
Philippe
P
Unité INSERM U581, Hôpital Henri Mondor, 51 Avenue du Mal de Lattre Tassigny, 94010 Creteil, France. philippe.grimbert@umontreal.ca
Audard
Vincent
V
Valanciute
Asta
A
Pawlak
André
A
Lang
Philippe
P
Guellaën
Georges
G
Sahali
Djillali
D
D017398:Alternative Splicing; D002648:Child; D015331:Cohort Studies; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D009404:Nephrotic Syndrome / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012313:RNA / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012008:Recurrence; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012694:Serine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology; D015854:Up-Regulation
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012694:Serine; D012313:RNA