No relationship between subchronic fluoride intake and DNA damage in Wistar rats.
Fluoride has been widely used in dentistry because it is an effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on the genetic apparatus. Genotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in peripheral blood, oral mucosa and brain cells in vivo. Male Wistar rats were exposed to sodium fluoride (NaF) at a 0, 7 and 100 ppm dose for drinking water during 6 weeks. The results pointed out that NaF did not contribute to the DNA damage in all cellular types evaluated as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to the correct evaluation of the potential health risk associated with dental agents exposure.
Ribeiro
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DA
Departamento de Patologia, Faculdade de Medicina de Botucatu, Núcleo de Avaliação Toxicogenética e Cancerígena (TOXICAN), Botucatu, UNESP, Brasil. ak92@hotmail.com
Marques
M E A
ME
de Assis
G F
GF
Anzai
A
A
Poleti
M L
ML
Salvadori
D M F
DM
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D018709:Statistics, Nonparametric; D014881:Water Supply / Q000032:analysis
D012969:Sodium Fluoride