Expression of kinin B1 receptors in the spinal cord of streptozotocin-diabetic rat.
Previous studies have reported cardiovascular and nociceptive responses after intrathecal injection of kinin B1 receptor (B1R) agonists in the model of streptozotocin (STZ)-diabetic rat (diabetic). The aim of this study was to measure the early up-regulation of B1R binding sites and mRNA in the thoracic spinal cord of diabetic and control rats. Data show significant increases of specific B1R binding sites in the dorsal horn of diabetic rats 2 days (+315%), 7 days (+303%) and 21 days (+181%) after STZ treatment. Levels of mRNA were significantly increased (+68%) at 2 and 7 days but not at 21 days. These data bring the first molecular evidence for an early up-regulation of B1R in the spinal cord of diabetic rat.
Ongali
Brice
B
Department of Physiology, Université de Montréal, Pavillon Paul-G.-Desmarais, 2960, Chemin de la Tour, Montréal, Québec, H3T 1J4, Canada.
Campos
Maria Martha
MM
Petcu
Mihai
M
Rodi
Donata
D
Cloutier
Frank
F
Chabot
Jean-Guy
JG
Thibault
Gaétan
G
Couture
Réjean
R
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization / Q000379:methods; D007456:Iodine Isotopes / Q000493:pharmacokinetics; D008297:Male; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D011859:Radiography / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D043783:Receptor, Bradykinin B1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013116:Spinal Cord / Q000378:metabolism*; D044005:Tetrahydroisoquinolines / Q000493:pharmacokinetics; D013997:Time Factors
D001786:Blood Glucose; D007456:Iodine Isotopes; D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D044005:Tetrahydroisoquinolines; C078665:HOE 140, desArg(10)-; D001920:Bradykinin