title: Polarization-resolved second-harmonic-generation optical coherence tomography in collagen.
abstract: We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We demonstrate this new technique on a skin sample from the belly of Icelandic salmon, acquiring polarization-resolved SHOCT and OCT images simultaneously.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. brian.applegate@duke.edu
authors.forename: Brian E
authors.initials: BE
authors.lastname: Applegate
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Rollins
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Izatt
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods*; D012474:Salmon; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure*; D013382:Subtraction Technique; D014018:Tissue Distribution; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
chemical_list: D003094:Collagen