Multiple POU-binding motifs, recognized by tissue-specific nuclear factors, are important for Dll1 gene expression in neural stem cells.
We cloned the 5'-flanking region of the mouse homolog of the Delta gene (Dll1) and demonstrated that the sequence between nucleotide position -514 and -484 in the 5'-flanking region of Dll1 played a critical role in the regulation of its tissue-specific expression in neural stem cells (NSCs). Further, we showed that multiple POU-binding motifs, located within this short sequence of 30bp, were essential for transcriptional activation of Dll1 and also that multiple tissue-specific nuclear factors recognized these POU-binding motifs in various combinations through differentiation of NSCs. Thus, POU-binding factors may play an important role in Dll1 expression in developing NSCs.
Nakayama
Kohzo
K
Department of Anatomy, Shinshu University, School of Medicine, Matsumoto, Nagano 390-8621, Japan. kohzona@gipac.shinshu-u.ac.jp
Nagase
Kazuko
K
Tokutake
Yuriko
Y
Koh
Chang-Sung
CS
Hiratochi
Masahiro
M
Ohkawara
Takeshi
T
Nakayama
Noriko
N
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D019521:Body Patterning / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009474:Neurons / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D050809:POU Domain Factors; D011485:Protein Binding; D051880:Receptors, Notch; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D050809:POU Domain Factors; D051880:Receptors, Notch; D014157:Transcription Factors; C064045:delta protein