title: Utility of molecular identification in opportunistic mycotic infections: a case of cutaneous Alternaria infectoria infection in a cardiac transplant recipient.
abstract: We report on a case of cutaneous infection caused by Alternaria infectoria in a cardiac transplant recipient. A rapid molecular diagnosis was obtained by sequence analysis of the internal transcribed spacer domain of the 5.8S ribosomal DNA region amplified from colonies developed on Sabouraud medium. Treatment consisted of a combination of systemic antifungal therapy, first with amphotericin B and then with itraconazole.
authors.affiliation: Servizio di Microbiologia, Azienda Ospedaliera di Verona, Ospedale Policlinico G. B. Rossi, Piazzale Scuro, 10, 37134 Verona, Italy. giuliana.locascio@azosp.vr.it
authors.forename: G
authors.initials: G
authors.lastname: Lo Cascio
authors.forename: M
authors.initials: M
authors.lastname: Ligozzi
authors.forename: L
authors.initials: L
authors.lastname: Maccacaro
authors.forename: R
authors.initials: R
authors.lastname: Fontana
mesh_terms: D000528:Alternaria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D003881:Dermatomycoses / Q000382:microbiology*; D016027:Heart Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016533:Mycological Typing Techniques; D009894:Opportunistic Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S