title: Regulation of chicken ccn2 gene by interaction between RNA cis-element and putative trans-factor during differentiation of chondrocytes.
abstract: CCN2/CTGF is a multifunctional growth factor. Our previous studies have revealed that CCN2 plays important roles in both growth and differentiation of chondrocytes and that the 3'-untranslated region (3'-UTR) of ccn2 mRNA contains a cis-repressive element of gene expression. In the present study, we found that the stability of chicken ccn2 mRNA is regulated in a differentiation stage-dependent manner in chondrocytes. We also found that stimulation by bone morphogenetic protein 2, platelet-derived growth factor, and CCN2 stabilized ccn2 mRNA in proliferating chondrocytes but that it destabilized the mRNA in prehypertrophic-hypertrophic chondrocytes. The results of a reporter gene assay revealed that the minimal repressive cis-element of the 3'-UTR of chicken ccn2 mRNA was located within the area between 100 and 150 bases from the polyadenylation tail. Moreover, the stability of ccn2 mRNA was correlated with the interaction between this cis-element and a putative 40-kDa trans-factor in nuclei and cytoplasm. In fact, the binding between them was prominent in proliferating chondrocytes and attenuated in (pre)hypertrophic chondrocytes. Stimulation by the growth factors repressed the binding in proliferating chondrocytes; however, it enhanced it in (pre)hypertrophic chondrocytes. Therefore, gene expression of ccn2 mRNA during endochondral ossification is properly regulated, at least in part, by changing the stability of the mRNA, which arises from the interaction between the RNA cis-element and putative trans-factor.
authors.affiliation: Biodental Research Center, Okayama University Dental School and Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8525, USA.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Mukudai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Takanori
authors.initials: T
authors.lastname: Eguchi
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.forename: Kyouji
authors.initials: K
authors.lastname: Nakao
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D055513:Connective Tissue Growth Factor; D005347:Fibroblasts / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D020871:RNA Stability / Q000502:physiology; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor