title: Identification, expression and phylogenetic analysis of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) Helicase gene.
abstract: The helicase gene from Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) was identified and localized in the 58.85-65.90 m.u. of the viral genomic map. This gene encodes a putative polypeptide of 1221 amino acids containing motifs homologous to those found in the helicase superfamily. Expression of the AgMNPV helicase was observed as early as 4h post-infection (p.i.) up until 10 h p.i. A unique early transcription initiation site was observed upstream a putative TATA box. Phylogenetic analysis of the helicase genes of 23 baculoviruses indicated that the AgMNPV helicase is closely related to the helicase genes from Epiphyas postvitanna multicapsid nucleopolyhedrovirus and Choristoneura fumiferana defective nucleopolyhedrovirus.
authors.affiliation: Laboratório de Virologia Molecular, Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier da Almeida Souza 200, SP, Brazil.
authors.forename: Leoberto
authors.initials: L
authors.lastname: de Lima
authors.forename: Francisco José Rivera
authors.initials: FJ
authors.lastname: Pinedo
authors.forename: Bergmann Morais
authors.initials: BM
authors.lastname: Ribeiro
authors.forename: Paolo Marinho Andrade
authors.initials: PM
authors.lastname: Zanotto
authors.forename: José Luiz Caldas
authors.initials: JL
authors.lastname: Wolff
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping; D004265:DNA Helicases / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D007915:Lepidoptera / Q000821:virology; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004265:DNA Helicases