title: [Diagnostic value of Gram stain for assessment of vaginal smears during pregnancy].
abstract: The main aim of this study was to compare the diagnostic value of Giemsa stained method with Gram stained method for the evaluation of vaginal smears among pregnant women. A study population comprised 111 pregnant between 6 and 30 weeks of gestation. The vaginal smears from every subject was diagnosed according to Giemsa and Gram stained method and micro-organisms were isolated by culture. In 29.3% cases diagnosed as normal flora (2a) on the basis of Giemsa method bacterial vaginosis was detected in Gram stains according to Spiegel's criteria and pathological microflora in concentration > or = 10(5) CFU/ml was cultured among 75.9% of them. Among 31.7% women who had grade 3a (abnormal) in Giemsa stains method normal flora was diagnosed on the basis on Gram's method and from 17.1% pregnant women from this group we did not isolated any pathogens. For evaluation of vaginal smears during pregnancy the Giemsa method should be replaced by Gram stained method.
authors.affiliation: Zaklad Mikrobiologii Lekarskiej, Uniwersytetu Medycznego w Lodzi.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wasiela
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Krzemiński
authors.forename: Wojciech
authors.initials: W
authors.lastname: Hanke
authors.forename: Jarosław
authors.initials: J
authors.lastname: Kalinka
mesh_terms: D001399:Azure Stains; D005260:Female; D005840:Gentian Violet; D006801:Humans; D010619:Phenazines; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000175:diagnosis / Q000382:microbiology
chemical_list: D001399:Azure Stains; C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet