Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina.
Two novel 21-residue antimicrobial peptides, arenicin-1 and arenicin-2, exhibiting activity against Gram-positive and Gram-negative bacteria and fungi, were purified from coelomocytes of marine polychaeta Arenicola marina (lugworm) by preparative gel electrophoresis and RP-HPLC. Molecular masses (2758.3 and 2772.3 Da) and complete amino acid sequences (RWCVYAYVRVRGVLVRYRRCW and RWCVYAYVRIRGVLVRYRRCW) were determined for each isoform. Each arenicin has one disulfide bond (Cys3-Cys20). The total RNA was isolated from the lugworm coelomocytes, RT-PCR and cloning were performed, and cDNA was sequenced. A 202-residue preproarenicin contains a putative signal peptide (25 amino acids) and a long prodomain. Arenicins have no structure similarity to any previously identified antimicrobial peptides.
Ovchinnikova
Tatiana V
TV
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str. 16/10, 117997 Moscow, Russia. ovch@ibch.ru
Aleshina
Galina M
GM
Balandin
Sergey V
SV
Krasnosdembskaya
Anna D
AD
Markelov
Mikhail L
ML
Frolova
Elena I
EI
Leonova
Yulia F
YF
Tagaev
Andrey A
AA
Krasnodembsky
Eugeny G
EG
Kokryakov
Vladimir N
VN
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides; D011077:Polychaeta / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers; D015801:Helminth Proteins; D010455:Peptides; C496888:arenicin-1, Arenicola marina; D012313:RNA