Differential polarization of immune responses by co-administration of antigens with chemokines.
Chemokines are key players in the elicitation of immune response, by selectively attracting subpopulations of immune cells to the site of antigen presentation. Therefore, they are natural candidates for modulating immune responses to antigens qualitatively and quantitatively. We have selected chemokines associated with different arms of the immune response, i.e. RANTES/CCL5, B-lymphocyte chemoattractant/CXCL13, and monocyte chemoattractant protein-1/CCL2, and co-injected DNA expression constructs encoding these chemokines with constructs encoding two HIV antigens, gp120 and gp160, in mice. We subsequently measured markers of both cellular and humoral immune responses, and found that these chemokines qualitatively influenced the outcome of immune responses to both antigens, essentially according to their predicted association to Th profiles. These results are relevant towards the engineering of novel vaccine and immune-based therapies, and point to chemokines as candidate adjuvant and immunomodulatory molecules.
Frauenschuh
Achim
A
Division of Basic Science, Institute of Human Virology, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA.
DeVico
Anthony L
AL
Lim
Siew Pheng
SP
Gallo
Robert C
RC
Garzino-Demo
Alfredo
A
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology; D018946:Chemokine CCL5 / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D018925:Chemokines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G; D007155:Immunologic Factors / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000906:Antibodies; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D018925:Chemokines; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D007074:Immunoglobulin G; D007155:Immunologic Factors; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma