Preparation of human hepatocellular carcinoma-targeted liposome microbubbles and their immunological properties.
OBJECTIVE
To prepare the human hepatocellular carcinoma-(HCC)-targeted liposome microbubbles and to investigate their immunological properties.


METHODS
Human hepatocarcinoma specific monoclonal antibody HAb18 was attached to the surface of home-made liposome microbubbles by static attraction to prepare the targeted liposome microbubbles. The combination of HAb18 with liposome microbubbles was confirmed by the slide agglutination test and immunofluorescent assay. Their immunological activity was measured by ELISA. Rosette formation test, rosette formation blocking test and immun-ofluorescent assay were used to identify the specific binding of targeted liposome microbubbles to SMMC-7721 hepatoma cells, and cytotoxicity assay was used to detect their effect on human hepatocytes.


RESULTS
The targeted liposome microbubbles were positive in the slide agglutination test and immunofluorescent assay. ELISA indicated that the immunological activity of HAb18 on the liposome microbubbles was similar to that of free HAb18. SMMC-7721 cells were surrounded by the targeting liposome microbubbles to form rosettes, while the control SGC-7901 gastric cancer cells were not. Proliferation of SMMC-7721 cells and normal human hepatocytes was not influenced by the targeted liposome microbubbles.


CONCLUSIONS
The targeted liposome microbubbles with a high specific biological activity have been successfully prepared, which specifically bind to human hepatocarcinoma cells, and are non-cytotoxic to hepatocytes. These results indicate that the liposome microbubbles can be used as a HCC-targeted ultrasound contrast agent that may enhance ultrasound images and thus improve the diagnosis of HCC, especially at the early stage.
Bian
Ai-Na
AN
Department of Ultrasonic Diagnosis, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China.
Gao
Yun-Hua
YH
Tan
Kai-Bin
KB
Liu
Ping
P
Zeng
Gong-Jun
GJ
Zhang
Xin
X
Liu
Zheng
Z
D000911:Antibodies, Monoclonal / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging* / Q000276:immunology*; D002455:Cell Division; D022781:Hepatocytes / Q000166:cytology / Q000276:immunology; D006801:Humans; D008081:Liposomes / Q000276:immunology; D008113:Liver Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology*; D045423:Microbubbles; D014463:Ultrasonography / Q000379:methods*
D000911:Antibodies, Monoclonal; D008081:Liposomes