[Prospects for practical application of substrate-binding modules of glycosyl hydrolases (A review)].
The properties of substrate-binding modules of glycosyl hydrolases have been reviewed. Variation of the properties of these modules makes them promising as components of chimeric proteins, which is a rapidly developing field of biotechnology. Examples of applying substrate-binding modules of glycosyl hydrolases to immobilization of proteins and whole cells on polysaccharides and purification of proteins are described. Promising methods for (1) detecting various compounds using hybrids of substrate-binding modules with antibodies and (2) locating polysaccharides in live tissues are reviewed as well.
Volkov
I Iu
IIu
Lunina
N A
NA
Velikodvorskaia
G A
GA
D000906:Antibodies; D001709:Biotechnology / Q000379:methods*; D040143:Cellulomonas / Q000201:enzymology; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D000906:Antibodies; D011134:Polysaccharides; D011506:Proteins; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases