Acid excreting mutants of yeast Saccharomyces cerevisiae.
Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the excreted protons are associated to particular sets of organic anions such as citrate, aconitate, succinate, fumarate or malate. These novel types of respiratory mutations seem to be located in genes operating in the Krebs or glyoxylate cycle.
Machnicka
B
B
Institute of Biotechnology and Environmental Protection, University of Zielona Góra, Monte Cassino 21b, 65-001 Zielona Góra, Poland.
Grochowalska
R
R
Boniewska-Bernacka
E
E
Słomińska
L
L
Lachowicz
T M
TM
D000143:Acids / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D010084:Oxidation-Reduction; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D000143:Acids; D011522:Protons; D014867:Water