title: Cytochemical location of urease in the cell wall of two different lichen phycobionts.
abstract: The enzyme urease has been located in the cell wall of recently isolated phycobionts from Evernia prunastri and Xanthoria parietina lichens. Cytochemical detection is achieved by producing a black, electron-dense precipitate of cobalt sulfide proceeding from CO(2) evolved from urea in the presence of cobalt chloride. Cellular fractionation reveals that about 80% of total urease activity was associated to the cell wall on both phycobionts whereas only 20% was recovered as soluble protein.
authors.affiliation: Laboratory of Plant Biology, Faculty of Biology, Complutense University, C/José Antonio Novias s/n, 28040 Madrid, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Millanes
authors.forename: B
authors.initials: B
authors.lastname: Fontaniella
authors.forename: M L
authors.initials: ML
authors.lastname: García
authors.forename: M T
authors.initials: MT
authors.lastname: Solas
authors.forename: C
authors.initials: C
authors.lastname: Vicente
authors.forename: M E
authors.initials: ME
authors.lastname: Legaz
mesh_terms: D002458:Cell Fractionation; D002473:Cell Wall / Q000201:enzymology* / Q000648:ultrastructure; D056890:Eukaryota / Q000201:enzymology* / Q000648:ultrastructure; D008011:Lichens / Q000201:enzymology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D013559:Symbiosis / Q000502:physiology*; D014510:Urease / Q000378:metabolism*
chemical_list: D014510:Urease