title: Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism.
abstract: The comprehensive bioconversion of aromatic compounds with a benzene ring to a picolinic acid was achieved with a recombinant Escherichia coli strain that expressed the six genes involved in biphenyl catabolism, these being the bphA1(2072)A2A3A4 genes encoding the evolved biphenyl dioxygenase, the bphB gene encoding dihydrodiol dehydrogenase, and the bphC gene encoding catechol 2,3-dioxygenase.
authors.affiliation: Department of Food and Nutrition, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan. kshindo@fc.jwu.ac.jp
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shindo
authors.forename: Ayako
authors.initials: A
authors.lastname: Osawa
authors.forename: Ryoko
authors.initials: R
authors.lastname: Nakamura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kagiyama
authors.forename: Shohei
authors.initials: S
authors.lastname: Sakuda
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shizuri
authors.forename: Kensuke
authors.initials: K
authors.lastname: Furukawa
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
mesh_terms: D001555:Benzene Derivatives / Q000378:metabolism; D001583:Benzoxazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism; D007189:Indans / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010848:Picolinic Acids / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D001583:Benzoxazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D007189:Indans; D007506:Iron-Sulfur Proteins; D010848:Picolinic Acids; D011804:Quinolines; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C074161:biphenyl-2,3-dioxygenase