title: [Mutation of mitochondrial DNA 4977 bp deletion in laryngeal squamous cell cancer].
abstract: OBJECTIVE
  The 4977 bp deletion of mitochondrial DNA is a molecular marker of cell ageing, and some solid tumors. This study was to detect deletion of mitochondrial DNA 4977 bp in laryngeal squamous cell cancer, and explore its correlation with differentiation of laryngeal cancer tissues.
  
  
  METHODS
  Thirty-five specimens were collected from patients with pathologically diagnosed laryngeal squamous cell cancer. The total DNA was amplified to identify the 4977 bp deletion of mitochondrial DNA by polymerase chain reaction (PCR), and PCR products were verified by direct sequencing.
  
  
  RESULTS
  The 4977 bp deletion detected in 20 of 35 cancer tissues (57.14%), including 5 of 7 with well differentiation, 15 of 24 with moderate differentiation, and 0 of 4 with poor differentiation. Mutation incidence of cancer tissues with well or moderate differentiation were significantly higher than that of cancer tissues with poor differentiation, but there was no significant difference between cancer tissues with well and moderate differentiation.
  
  
  CONCLUSIONS
  The 4977 bp deletion of mitochondrial DNA was detected in laryngeal squamous cell cancer tissues, it might be relates to differentiation of cancer tissues.
authors.affiliation: Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430 022, P.R. China.
authors.forename: Yue-Chen
authors.initials: YC
authors.lastname: Han
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Kong
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiong
authors.initials: X
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm