title: Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry.
abstract: Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is approximately 0.3 degrees C (80 microM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Dreher
authors.forename: Martyna
authors.initials: M
authors.lastname: Elas
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ichikawa
authors.forename: Eugene D
authors.initials: ED
authors.lastname: Barth
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Chilkoti
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Rosen
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Halpern
authors.forename: Mark
authors.initials: M
authors.lastname: Dewhirst
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000528:radiation effects; D004549:Elastin / Q000737:chemistry* / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006358:Hot Temperature; D008422:Materials Testing; D009589:Nitrogen Oxides / Q000737:chemistry* / Q000528:radiation effects; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013817:Thermography / Q000295:instrumentation* / Q000379:methods; D013821:Thermometers
chemical_list: D001672:Biocompatible Materials; D009589:Nitrogen Oxides; D010455:Peptides; D004549:Elastin; C039900:nitroxyl