Adhesion of Enterococcus faecalis in the nonculturable state to plankton is the main mechanism responsible for persistence of this bacterium in both lake and seawater.
The presence of enterococci in lake and seawater in an 18-month survey comparing molecular (PCR and quantitative PCR) and culture methods was evaluated, as well as the possibility that zooplankton could act as reservoirs for enterococci. Samples of both water and zooplankton were collected monthly from a Lake Garda site and an Adriatic Sea site. In lake water, the positive samples numbered 13 of 54 (24%) by culture and 32 of 54 (59%) when PCR was applied. In seawater, they numbered 0 of 51 by culture and 18 of 51 (35%) by PCR. Enterococci were found either totally bound to plankton or totally in water, depending on the presence or absence of plankton, respectively. These results clearly indicate that the PCR assay is a powerful tool for detecting fecal indicators and pathogens in the environment, thus providing a much more sensitive method than culture.
Signoretto
Caterina
C
Sezione di Microbiologia, Dipartimento di Patologia, Università di Verona, Verona, Italy.
Burlacchini
Gloria
G
Lleò
Maria del Mar
MM
Pruzzo
Carla
C
Zampini
Massimiliano
M
Pane
Luigi
L
Franzini
Giorgio
G
Canepari
Pietro
P
D000818:Animals; D001422:Bacterial Adhesion; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005618:Fresh Water / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology*; D015048:Zooplankton / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial