[Study on sero-pharmacology of fresh Gecko Swinhonis Gunther freeze-dried powder in inducing cell apoptosis of C6 glioma cells in mice].
OBJECTIVE
To explore the effect of Gecko Swinhonis Gunther freeze-dried powder (GFP) in inducing apoptosis of C6 glioma cells.


METHODS
Thirty mice were randomly divided into three groups and treated with intraperitoneally injection of cisplatin, orally taken GFP or distilled water respectively. After treatment for 20 days, the serum of mice was collected for treating the C6 glioma cells. The apoptosis state of cells was observed by morphological examination, flow cytometry and TUNEL method, and the cell apoptosis related gene expression of bcl-2 and bax were determined by S-P immunocytochemical assay.


RESULTS
The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method. Comparison between the GFP treated group and the blank control group on intracellular bcl-2 gene expression showed no difference, but bax gene expression was higher in the GFP treated group.


CONCLUSIONS
GFP contained serum could induce C6 glioma cell apoptosis, its mechanism might be related with the up-regulation of bax gene.
Song
Ping
P
The First Hospital of Peking University, Beijing 100034.
Wang
Xue-mei
XM
Xie
Shuang
S
Pu
Dingfang
D
Fu
Hong
H
Liu
Gengxin
G
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005910:Glioma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D044967:Serum; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
D000970:Antineoplastic Agents; C494826:BAX protein, human; C494827:Bax protein, mouse; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein