Flow cytometry measurements of intracellular Ca2+ in K 562 cells.
The aim of this study is to show the intracellular Ca2+ changes induced by methotrexate, a cell cycle inhibitor, in order to prove the intracellular Ca2+ implication in cell proliferation processes. The fluorescent dye used to measure this parameter is Fluo-3, a fluorescein derivative useful for measuring the kinetics of Ca2+ transitions, waves and oscillations. The alterations of Ca2+ concentration proper to K562 cells have been studied, using methotrexate as a cell activation factor, both as direct effects on the cell response (1-15 minutes), and effects in time (24h.), using methotrexate in the culture medium. The K562 response at Ca2+ level in time remained constant for the cells grown in methotrexate medium, showing a slight increase in the control medium. We proved that methotrexate, a cell cycle inhibitor decreased the cytoplasmatic Ca2+ concentration in K562 cells, soon after adding methotrexate both to T0 and T1.
Olariu
L
L
S.C. Biotehnos S.A., Dumbrava Roşie, 18, Bucharest 70254, Romania.
Dumitriu
B
B
Neagu
M
M
Manda
G
G
D000814:Aniline Compounds; D002118:Calcium / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D020014:K562 Cells; D008727:Methotrexate / Q000494:pharmacology; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium; D008727:Methotrexate