[Study on optimizing the extraction process of total free organic acids in Pinellia ternate Breit].
OBJECTIVE
To optimize the extraction process of total free organic acids in Pinellia ternate Breit.


METHODS
The optimum extraction was investigated by total free organic acids determined by direct potential titration. The concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the experiment.


RESULTS
The optimum extraction process was adding 10 times amount of 75% ethanol, refluxing for 2 times, 1 h each time.


CONCLUSIONS
This extraction process shows higher yield of total free organic acids in Pinellia ternate Breit and is available for industrial production.
Wang
Mei
M
Research Center for Marine Drugs, Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
Wu
Hao
H
Xu
Fengqing
F
Zhang
Yongtai
Y
D000143:Acids / Q000032:analysis / Q000302:isolation & purification*; D000704:Analysis of Variance; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol; D010169:Palmitic Acids / Q000032:analysis / Q000302:isolation & purification; D030021:Pinellia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000032:analysis; D013386:Succinates / Q000032:analysis / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods*; D013995:Time
D000143:Acids; D004365:Drugs, Chinese Herbal; D010169:Palmitic Acids; D013386:Succinates; D000431:Ethanol; D012972:Sodium Hydroxide