Simultaneous residue measurement of pendimethalin, isopropalin, and butralin in tobacco using high-performance liquid chromatography with ultraviolet detection and electrospray ionization/mass spectrometric identification.
A simultaneous residue analysis of pendimethalin, isopropalin, and butralin in tobacco was developed with high-performance liquid chromatography with ultraviolet monitoring and electrospray ionization mass spectrometry (HPLC-UV-ESI/MS). The herbicide residues of pendimethalin, isopropalin, and butralin in tobaccos were extracted by ultrasonication with ethyl acetate, followed by a cleanup procedure with gel permeation chromatography. The three herbicides were separated within 15 min using a LiChrosorb 18 column with methanol-10 mmol/L ammonium acetate (85:15, v/v) as the eluent. The limits of quantitation, using HPLC-UV, were ca. 0.05, 0.08, and 0.06 mg/kg for pendimethalin, isopropalin, and butralin, respectively, whereas the overall recoveries ranged from 77.5 to 91.8%. The proposed method has been successfully applied to measure 300 real samples, and the residue profiles of three herbicides in tobacco samples were obtained and evaluated.
Liu
Hongxia
H
Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, People's Republic of China. liuhongxia999@371.net
Ding
Chenghua
C
Zhang
Shusheng
S
Liu
Huimin
H
Liao
Xincheng
X
Qu
Lingbo
L
Zhao
Yufen
Y
Wu
Yangjie
Y
D000814:Aniline Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014026:Nicotiana / Q000737:chemistry*
D000814:Aniline Compounds; D006540:Herbicides; D010573:Pesticide Residues; C008200:isopropalin; C006852:butralin; C030856:pendimethalin