"In vitro" effects of different arsenic compounds on PBMC (preliminary study).
Aim of this investigation was to compare the effects of 10(-4) M and 10(-7) M As compounds on spontaneous and PHA stimulated PBMC proliferation and IFN-gamma and TNF-alpha release. The inhibitory effect of the 10(-4) M As salts was in the following order: momo-methyl-arsinous acic (MMAs(III)) > sodium arsenite (As(III)) > tetraphenyl arsonium chloride (As(V)) > sodium arsenate (As(V)) > potassium- and sodium-esa-fluorum arsenate (As(V)) > dimethyl arsinic acid (DMAs(V)), while monomethyl-arsonic-acid (MMAs(V)) and arsenobetaine did not exert immune effects. 10(-7) M MMAs(III) stimulated the spontaneous PBMC proliferation, while As(III) and DMAs(V) enhanced the PHA stimulated PBMC proliferation. This study shows that the immune effects of As salts depends on speciation; moreover, the immunotoxicity of inorganic arsenic in part depends on the intracellular bio-synthesis of MMAs(III) from MMAs(V).
Di Giampaolo
L
L
Section of Occupational Medicine, Allergology and Clinical Immunology, Department of Medicine and Science of Ageing, "G. D'Annunzio" University of Chieti and Pescara, Via dei Vestini 66100, Chieti, Italy.
Di Gioacchino
M
M
Qiao
Niu
N
Travaglini
P
P
D'Intino
A
A
Kouri
M
M
Ponti
J
J
Castellani
M L
ML
Reale
M
M
Gabriele
E
E
Boscolo
P
P
D000818:Animals; D001149:Arsenates / Q000009:adverse effects; D001151:Arsenic / Q000009:adverse effects*; D001152:Arsenicals / Q000009:adverse effects*; D018053:Arsenites / Q000009:adverse effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D017670:Sodium Compounds / Q000009:adverse effects; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D010835:Phytohemagglutinins; D017670:Sodium Compounds; D014409:Tumor Necrosis Factor-alpha; C009439:tetraphenylarsonium; C017947:sodium arsenite; C009277:sodium arsenate; D007371:Interferon-gamma; D001151:Arsenic; C038992:arsenobetaine