title: Induction of apoptosis by maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) in human promyelotic leukemia HL-60 cells.
abstract: We have investigated the effects of maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) on DNA of human promyelotic HL-60 leukemia cells. It was found that extent of induction of apoptosis by maackiain was larger than that by trifolirhizin in human leukemia HL-60 cells. Morphological changes showing apoptotic bodies were observed in the HL-60 cells treated with maackiain and trifolirhizin. The fragmentations of DNA by maackiain and trifolirhizin to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the HL-60 cells. The data of the present study show that the suppressions by maackiain and trifolrhizin of growth of the HL-60 cells result from the induction of apoptosis by these compounds, and that the extent of growth suppression and induction of apoptosis by maackiain was greater than that by the glycoside (trifolirhizin).
authors.affiliation: Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan.
authors.forename: Yue
authors.initials: Y
authors.lastname: Aratanechemuge
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Hibasami
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Katsuzaki
authors.forename: Kunio
authors.initials: K
authors.lastname: Imai
authors.forename: Takashi
authors.initials: T
authors.lastname: Komiya
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004247:DNA / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D036343:Pterocarpans / Q000737:chemistry / Q000494:pharmacology*; D000094162:Phytoalexins
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004247:DNA; D036343:Pterocarpans; C001449:inermin; D000094162:Phytoalexins