title: Streptomyces glauciniger sp. nov., a novel mesophilic streptomycete isolated from soil in south China.
abstract: A polyphasic study was undertaken to establish the taxonomic status of a soil isolate. The organism, strain FXJ14(T), was found to have chemical and morphological properties characteristic of streptomycetes. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120 nt variable gamma-region of the 16S rRNA molecule showed that it formed a distinct phyletic line within the range of variation encompassed by the genus Streptomyces. The sharp separation of the organism from representatives of the genus Streptomyces was strengthened by the fact that its BOX-PCR and RFLP of 16S rDNA-ITS fingerprints differed from those of over 450 recognized Streptomyces species. The isolate also had a profile of phenotypic properties that readily distinguished it from the genotypically close type strains. It is evident from the combination of genotypic and phenotypic data that strain FXJ14(T) (=AS 4.1858(T)=JCM 12278(T)=LMG 22082(T)) should be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces glauciniger sp. nov. is proposed.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lanoot
authors.affiliation: BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Carlos
authors.initials: C
authors.lastname: Rodriguez
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: BCCM/LMG Bacteria Collection, Department of Biochemistry, Physiology and Microbiology, University Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S