Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets.
Cytokinesis involves temporally and spatially coordinated action of the cell cycle and cytoskeletal and membrane systems to achieve separation of daughter cells. To dissect cytokinesis mechanisms it would be useful to have a complete catalog of the proteins involved, and small molecule tools for specifically inhibiting them with tight temporal control. Finding active small molecules by cell-based screening entails the difficult step of identifying their targets. We performed parallel chemical genetic and genome-wide RNA interference screens in Drosophila cells, identifying 50 small molecule inhibitors of cytokinesis and 214 genes important for cytokinesis, including a new protein in the Aurora B pathway (Borr). By comparing small molecule and RNAi phenotypes, we identified a small molecule that inhibits the Aurora B kinase pathway. Our protein list provides a starting point for systematic dissection of cytokinesis, a direction that will be greatly facilitated by also having diverse small molecule inhibitors, which we have identified. Dissection of the Aurora B pathway, where we found a new gene and a specific small molecule inhibitor, should benefit particularly. Our study shows that parallel RNA interference and small molecule screening is a generally useful approach to identifying active small molecules and their target pathways.
Eggert
Ulrike S
US
Department of Systems Biology, Harvard Medical School Boston, Massachusetts, USA. ulrike_eggert@hms.harvard.edu <ulrike_eggert@hms.harvard.edu>
Kiger
Amy A
AA
Richter
Constance
C
Perlman
Zachary E
ZE
Perrimon
Norbert
N
Mitchison
Timothy J
TJ
Field
Christine M
CM
D000818:Animals; D064127:Aurora Kinases; D017173:Caenorhabditis elegans; D002460:Cell Line; D048749:Cytokinesis; D004330:Drosophila; D005559:Formamides / Q000494:pharmacology; D005821:Genetic Techniques; D016678:Genome; D023281:Genomics / Q000379:methods*; D008856:Microscopy, Fluorescence; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011720:Pyrazoles / Q000138:chemical synthesis / Q000494:pharmacology; D034622:RNA Interference; D012441:Saccharomyces cerevisiae
D005559:Formamides; C509894:N'-2-(1-(3-chloro-4-fluorophenyl)-4-cyano-1H-pyrazol-5-yl)-N,N-dimethyliminoformamide; D011720:Pyrazoles; C031066:formamide; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases