[Isolation of plant insulin from Momordica charantia seeds by gel filtration and RP-HPLC].
Hypoglycemic polypeptide (PA) was extracted from Momordica charantia seeds with organic acid and ethanol and purified with Sephadex G-50 gel filtration and RP-HPLC. PA was judged as plant insulin on the base of the analysis of its SDS-PAGE electrophoresis and amino acid composition.
Sheng
Qingkai
Q
School of Food Science and Technology, Southern Yangtze University, Wuxi 214036.
Yao
Huiyuan
H
Xu
Huajun
H
Ling
Xiaoyun
X
He
Ting
T
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007328:Insulin / Q000737:chemistry / Q000302:isolation & purification*; D029777:Momordica charantia / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D000596:Amino Acids; D007328:Insulin; D010455:Peptides