title: Localization of centrins in the hypotrich ciliate Paraurostyla weissei.
abstract: Centrins are ubiquitous cytoskeletal proteins that are generally associated with the centrosome and form large cytoskeletal networks in protists. To obtain more data on the respective role of different centrin proteins, we studied their distribution and behavior in one ciliate species, Paraurostyla weissei, using specific antibodies. In this species, only two major proteins of 21 and 24 kDa corresponding to centrins, were identified by 1D and 2D electrophoresis. Immunofluorescence analysis showed that these two proteins displayed non-overlapping localization in the interphase cell and during morphogenesis. Both centrin proteins localize on the fibrous network linking the oral basal bodies in the interphase cell and in the form of marginal dots, which correspond to the proximal ends of the striated rootlets; the 21 kDa centrin was also detected within the basal bodies, whereas the 24 kDa centrin allowed identifying new structures, the frontal dashes. During morphogenesis, the 21 kDa centrin locates at the basal bodies, while the 24 kDa centrin is detected along the striated rootlets and in close association with the basal bodies pairs. These data are discussed in terms of the potential roles of the two centrins in different cellular functions.
authors.affiliation: Biologie Cellulaire 4, Bâtiment 444, Université Paris XI, 91 405 Orsay, France. michel.lemullois@ibaic.u-psud.fr
authors.forename: Michel
authors.initials: M
authors.lastname: Lemullois
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Fryd-Versavel
authors.forename: Anne
authors.initials: A
authors.lastname: Fleury-Aubusson
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D016798:Ciliophora / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D007399:Interphase; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009024:Morphogenesis; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000913:Antibodies, Protozoan; D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D015800:Protozoan Proteins; C056608:caltractin