[Isolation and cultivation of mouse bone marrow mesenchymal stem cells].
To establish the best condition of isolation and cultivation of mouse bone marrow mesenchymal stem cells (BMSCs) in vitro, we isolated BMSCs from BALB/C mouse using density gradient centrifugation and adherent selecting. The effects of different centrifuge power, adherent time, serum concentration and cell density on the isolation and cultivation of BMSCs were investigated. The best isolating condition is: 500g x 30min, 24 hours adhering. The best cell density is 12-20 x 10(5)/ml of primary cells and 6.4-25.6 x 10(4)/ml of secondary cells. The best serum concentration is 10%. More than 90% of subcultured cells were adhesive in 8 hours. Thus we have established a cell biological method of isolation and cultivation of BMSCs.
Zhang
Yi
Y
West China Hospital of Sichuan University, Biotherapy of Human Diseases of Ministry of Education, Key Laboratory of Molecular Biology for Infectious Diseases of Sichuan Province, Chengdu 610041, China.
Zhao
Liansan
L
Tang
Hong
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D003470:Culture Media; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D044967:Serum
D003470:Culture Media