title: HPLC profiling and quantification of active principles in leaves of Hedera helix L.
abstract: Ivy (Hedera helix L., Araliaceae), is an evergreen medicinal and ornamental plant. Depending on leaf polymorphism different shaped ivy leaves were extracted and subsequently analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Quantitative determination of its most prominent saponins hederacoside C (1) and alpha-hederin (2) from different ivy leaf extracts were detected, validated and optimized for quick profiling. The linearity of response, repeatability and reproducibility of the applied RP-HPLC method are reported.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Chemistry and Pharmacy, University of Regensburg, Regensburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Demirci
authors.forename: M
authors.initials: M
authors.lastname: Goppel
authors.forename: F
authors.initials: F
authors.lastname: Demirci
authors.forename: G
authors.initials: G
authors.lastname: Franz
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D035981:Hedera / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
chemical_list: D010936:Plant Extracts; D012503:Saponins; C482302:hederacoside C; D009828:Oleanolic Acid