Indirect photometric assay determination of gabapentin in bulk drug and capsules by capillary electrophoresis.
A capillary electrophoresis (CE) method was developed for the assay determination of gabapentin (GBP) in bulk drug and capsules. Separation was carried out on 74cm (62.5cm effective length) x 75microm i.d. fused silica capillary by applying a potential of -20kV at ambient temperature. Background electrolyte (BGE) consisting of 5mM 5-sulphosalicylic acid and 0.5mM cetyltrimethylammonium bromide (CTAB), pH 11.0 was employed for the separation. Indirect method of UV detection was performed at a wavelength of 215nm using sulphosalicylate ion as a chromophore. A linear calibration curve was obtained over a concentration range 20-200microg/ml of GBP in deionized water with a correlation coefficient (r) of 0.9998. Recoveries were shown to be >/=98% both in bulk drug and capsules with standard deviation (S.D.) </=2.3%. No placebo (matrix) peaks were observed at the migration times of GBP and L-glutamic acid (internal standard, I.S.).
Sekar
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R
Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad 500 007, India. seker@mail.iictnet.com
Azhaguvel
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S
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002214:Capsules; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D000077206:Gabapentin; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D010783:Photometry / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000737:chemistry
D000588:Amines; D002214:Capsules; D003509:Cyclohexanecarboxylic Acids; D004364:Pharmaceutical Preparations; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin