AF1q, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron-like cells.
Three cell groups, neural stem/progenitor cells (NS/PCs) dissociated from the embryonic day 11 (E11) rodent cerebral cortex, expanded NS/PC cultures, and cultured neurons from E15, were used to conduct a genomic study with differential display (DD). The mouse Af1q, homologue of human AF1q, was found to be significantly up-regulated during the neuronal production from NS/PCs. The ectopic expression of human AF1q triggered the expression of the neuronal marker TuJ1 in non-neuronal human embryonic kidney (HEK) cells.
Lin
Hsingchi J
HJ
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA. jlin@cbmse.nrl.navy.mil
Shaffer
Kara M
KM
Sun
Zairen
Z
Jay
Gilbert
G
He
Wei-wu
WW
Ma
Wu
W
D000818:Animals; D015415:Biomarkers; D001798:Blood Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007668:Kidney / Q000166:cytology*; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D011518:Proto-Oncogene Proteins; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014162:Transfection; D014404:Tubulin / Q000235:genetics
D015415:Biomarkers; D001798:Blood Proteins; C091736:MLLT11 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D014404:Tubulin; C462941:beta3 tubulin, mouse