title: Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film.
abstract: We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.
authors.affiliation: Clear Solutions Biotech, Inc., Stony Brook, New York, USA.
authors.forename: Angela P
authors.initials: AP
authors.lastname: Kim
authors.forename: Paige
authors.initials: P
authors.lastname: Yellen
authors.forename: Yang H
authors.initials: YH
authors.lastname: Yun
authors.forename: Evren
authors.initials: E
authors.lastname: Azeloglu
authors.forename: Weiliam
authors.initials: W
authors.lastname: Chen
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003432:Cross-Linking Reagents; D016503:Drug Delivery Systems / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D014453:Glucuronosyltransferase / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics*; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics*; D014162:Transfection / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C491099:Has2 protein, mouse; D000076002:Hyaluronan Synthases