title: Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice.
abstract: We examined possible roles of mast cells in cutaneous wound healing using mast cell deficient (W/Wv) mice and their normal littermates (+/+). A round full-thickness wound was made on the back skin of these mice. The wounds closed completely within 20 days, and there was no difference in wound contraction between +/+ and W/Wv mice during the wound healing. While either chymase or tryptase activities were hardly detectable in W/Wv mice, chymase activities decreased at the impaired sites and recovered to the control level within 20 days in +/+ mice. Tryptase activities were higher than the control level on day 15 and day 20 in +/+ mice. Histological observations on day 15 and day 20 in +/+ mice revealed that mast cells were abundant at the wound edges but absent at the center. The latent and the active forms of MMP-2 and MMP-9 increased on day 10 and day 15 but recovered nearly to control levels on day 20 in both mice groups. The hydroxyproline contents in W/Wv mice were significantly higher than those in +/+ mice on day 15 and day 20. Furthermore, histological observations revealed that the collagen aggregation at the wound edges was tighter and less interwoven in W/Wv mice compared with +/+ mice. These results suggest that mast cells accumulated at the wound edge may participate in tissue remodeling in the late phase of wound healing.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata 573-0101, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iba
authors.forename: Aya
authors.initials: A
authors.lastname: Shibata
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kato
authors.forename: Tohru
authors.initials: T
authors.lastname: Masukawa
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism; D053818:Chymases; D003094:Collagen / Q000378:metabolism*; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D012038:Regeneration / Q000187:drug effects; D012697:Serine Endopeptidases / Q000378:metabolism; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D053802:Tryptases; D014945:Wound Healing / Q000502:physiology*
chemical_list: C509190:Tpsb2 protein, mouse; D003094:Collagen; D012697:Serine Endopeptidases; D053818:Chymases; C509222:Tpsab1 protein, mouse; D053802:Tryptases; D006909:Hydroxyproline