title: Effects of deafferentation on the size and myosin phenotype of muscle fibers on stretching of the rat soleus muscle in conditions of gravitational unloading.
abstract: The aim of the present work was to assess the contributions of the reflex and local components to preventing decreases in the size and changes in the ratio of fibers containing the slow and fast isoforms of myosin heavy chains during chronic stretching of a postural muscle in rats in conditions of gravitational unloading. A unilateral surgical deafferentation method was used. The results demonstrated that deafferentation of the hindlimb had no effect on preventing reductions in muscle fiber size in conditions of chronic muscle stretching in conditions of gravitational unloading. The results obtained from these experiments did not support the hypothesis that the predominant contribution to preventing the development of atrophic changes comes from activation of muscle afferents in chronic stretching of the unloaded muscle. Deafferentation of both suspended animals and those with normal motor activity led to increases in the proportion of soleus muscle fibers containing the slow isoforms of myosin heavy chain.
authors.affiliation: State Scientific Center of the Russian Federation/Institute of Medical-Biological Problems, Russian Academy of Sciences, 761 Khoroshevskoe Chaussee, 123007 Moscow, Russia.
authors.forename: T L
authors.initials: TL
authors.lastname: Nemirovskaya
authors.forename: B S
authors.initials: BS
authors.lastname: Shenkman
authors.forename: A M
authors.initials: AM
authors.lastname: Mukhina
authors.forename: Ya Yu
authors.initials: YY
authors.lastname: Volodkovich
authors.forename: M M
authors.initials: MM
authors.lastname: Sayapina
authors.forename: E
authors.initials: E
authors.lastname: Brattseva
authors.forename: O
authors.initials: O
authors.lastname: Larina
mesh_terms: D000818:Animals; D019417:Hindlimb Suspension / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D009121:Muscle Denervation / Q000379:methods*; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000502:physiology*; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000502:physiology*; D009929:Organ Size; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical / Q000379:methods; D018474:Weightlessness Simulation / Q000379:methods*
chemical_list: D018995:Myosin Heavy Chains