Homocysteine induces production of monocyte chemoattractant protein-1 and interleukin-8 in cultured human whole blood.
OBJECTIVE
To investigate whether increased plasma L-homocysteine (Hcy) level could promote monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in cultured whole blood.


METHODS
Human whole blood or different type of peripheral blood cells from health volunteers were incubated with Hcy and/or the inhibitors. MCP-1 and IL-8 level were measured by ELISA assay.


RESULTS
Hcy 10-1000 micromol/L induced production of MCP-1 and IL-8 in cultured human whole blood (P<0.05). The major cellular source of these chemokines comed from monocytes. Meanwhile,Hcy also promoted the upregulation of MPO level even at the 10 micromol/L in the cultured whole blood. The intracellular ROS, particular the OH. radicals, play extremely important role in the Hcy-induced MCP-1 and IL-8 production.


CONCLUSIONS
Increased Hcy level in plasma (hyperhomocysteinemia) induced MCP-1 and IL-8 secretion in cultured human whole blood, especially in monocytes via oxidative stress mechanism.
Zeng
Xiao-kun
XK
Institute of Vascular Medicine, Peking University Third Hospital, China.
Remick
Daniel G
DG
Wang
Xian
X
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D018932:Chemokine CCL2; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D006710:Homocysteine; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase