title: Interferon-gamma response of PBMC indicates productive pseudorabies virus (PRV) infection in swine.
abstract: In Chinese Meishan/German Landrace cross-bred swine F2 generation interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (PRV) infection. This reactivity was compared with the reactivity of naïve PBMC. Significant IFN-gamma production was determined in ELISA and ELISPOT only after in vitro PBMC re-stimulation with PRV and not with the closely related bovine herpesvirus BHV-1. The PRV-specific IFN-gamma secretion from re-stimulated PBMC showed high levels 6 days after infection, before the presence of serum antibodies, and it persisted at a high level over a 3 months period. The response of a group of eight piglets infected intranasally with PRV varied. Only two animals showed the expected typical fever response. PRV specific IFN-gamma production by PBMC clearly indicated that infection had occurred. Early significant IFN-gamma production by primed PBMC turned out to be a reliable and specific ex vivo marker for cellular response against productive PRV infection in swine before antibody formation.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Institute of Immunology, Paul-Ehrlich-Strasse 28, D72076 Tübingen, Germany.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Hoegen
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.forename: Marlene
authors.initials: M
authors.lastname: Röttgen
authors.forename: Hanns-Joachim
authors.initials: HJ
authors.lastname: Rziha
authors.forename: Hermann
authors.initials: H
authors.lastname: Geldermann
authors.forename: Gerald
authors.initials: G
authors.lastname: Reiner
authors.forename: Eberhard
authors.initials: E
authors.lastname: Pfaff
authors.forename: Mathias
authors.initials: M
authors.lastname: Büttner
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology*; D013552:Swine / Q000276:immunology; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007371:Interferon-gamma