title: Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin.
abstract: The interaction between monoammonium glycyrrhizinate (MAG) and bovine serum albumin (BSA) were studied by fluorescence and absorption spectroscopy. The quenching mechanism of fluorescence of bovine serum albumin by monoammonium glycyrrhizinate was discussed. The binding sites number n and apparent binding constant K were measured by fluorescence quenching method. The thermodynamic parameters DeltaH degrees , DeltaG degrees , DeltaS degrees at different temperatures were calculated. The distance r between donor (bovine serum albumin) and acceptor (monoammonium glycyrrhizinate) was obtained according to Forster theory of non-radiation energy transfer. The results of synchronous fluorescence spectra and UV-vis absorption spectra show that the conformation of bovine serum albumin has been changed.
authors.affiliation: Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Jia-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
authors.forename: Song-Sheng
authors.initials: SS
authors.lastname: Qu
mesh_terms: D000818:Animals; D002417:Cattle; D004347:Drug Interactions; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000644:Quaternary Ammonium Compounds; D012710:Serum Albumin, Bovine; D019695:Glycyrrhizic Acid