Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance.
Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca2+ signals. This diversity results in different physiological responses to particular stimuli. Gm-CaM-4 and -5 are two divergent calmodulin isoforms from the soybean (Glycine max) that have been reported to be involved in plant disease resistance. However, little is known about the pathway by which these specific isoforms transduce the defense signal and up-regulate pathogenesis-related (PR) genes. Here we report that overexpression of GmCaM-4/-5 induces constitutive PR gene expression and enhances disease resistance in wild-type Arabidopsis, but not in the nim1 mutant of Arabidopsis. GmCaM-4/-5 also appear to activate trans-acting elements that bind to cis-acting elements in the Arabidopsis PR-1 promoter. Thus up-regulation of PR genes by these GmCaM isoforms is dependent on NIM1 (Non immunity 1) and unknown transcription factors.
Park
Chan Young
CY
Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Heo
Won Do
WD
Yoo
Jae Hyuk
JH
Lee
Ju Huck
JH
Kim
Min Chul
MC
Chun
Hyun Jin
HJ
Moon
Byeong Cheol
BC
Kim
Ihn Hyeong
IH
Park
Hyeong Cheol
HC
Choi
Man Soo
MS
Ok
Hyun Mi
HM
Cheong
Mi Sun
MS
Lee
Sang Min
SM
Kim
Ho Soo
HS
Lee
Kon Ho
KH
Lim
Chae Oh
CO
Chung
Woo Sik
WS
Cho
Moo Je
MJ
D017360:Arabidopsis / Q000276:immunology; D029681:Arabidopsis Proteins / Q000502:physiology; D002147:Calmodulin / Q000235:genetics / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics*; D030821:Plants, Genetically Modified; D020033:Protein Isoforms; D013025:Glycine max / Q000235:genetics; D015533:Transcriptional Activation; D014162:Transfection
D029681:Arabidopsis Proteins; D002147:Calmodulin; C105496:NPR1 protein, Arabidopsis; D020033:Protein Isoforms