title: Expression microdissection: operator-independent retrieval of cells for molecular profiling.
abstract: Tissue microdissection is an important method for the study of disease states. However, it is difficult to perform high-throughput molecular analysis with current techniques. We describe here a prototype version of a novel technique (expression microdissection) that allows for the procurement of desired cells via molecular targeting. Expression microdissection (xMD) offers significant advantages over available methods, including an increase in dissection speed of several orders of magnitude. xMD may become a valuable tool for investigators studying cancer or other disease states in patient specimens and animal models.
authors.affiliation: Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Tangrea
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Chuaqui
authors.forename: John W
authors.initials: JW
authors.lastname: Gillespie
authors.forename: Mamoun
authors.initials: M
authors.lastname: Ahram
authors.forename: Gallya
authors.initials: G
authors.lastname: Gannot
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Wallis
authors.forename: Carolyn J M
authors.initials: CJ
authors.lastname: Best
authors.forename: W Marston
authors.initials: WM
authors.lastname: Linehan
authors.forename: Lance A
authors.initials: LA
authors.lastname: Liotta
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Pohida
authors.forename: Robert F
authors.initials: RF
authors.lastname: Bonner
authors.forename: Michael R
authors.initials: MR
authors.lastname: Emmert-Buck
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D008846:Micromanipulation / Q000379:methods*; D040901:Proteomics