title: [Human soleus fibers contractile characteristics and sarcomeric cytoskeletal proteins after gravitational unloading. Contribution of support stimulus].
abstract: The effects of support withdrawal and support stimulation on the contractile characteristics of human soleus fibers and cellular factors which influence them were studied. The experimental model of the "dry" head-out water immersion was used in the study. In this model, the hydrostatic pressure on different sites of the body surface are equal so that the experimental conditions are close to the complete supportlessness. A 7-day exposure to dry immersion resulted in a decrease in the maximal isometric tension of the skinned fibers, a decline in the myofibrillar Ca2+-sensitivity, and the relative loss of the titin and nebulin content. A significant decrease in the percentage of fibers containing slow myosin heavy chains was also observed after dry immersion. The application of the mechanical stimulator influencing the plantar support zones with a pressure of 0.2 +/- 0.15 kg/cm2 6 times a day for 20 minutes of each hour brought about a complete prevention of the above listed effects of dry immersion. The data obtained allow one to conclude that the decline in maximal tension and Ca2+-sensitivity as well as myosin shift and loss of sarcomeric cytoskeletal proteins are associated with the support withdrawal during the exposure to dry immersion.
authors.forename: B S
authors.initials: BS
authors.lastname: Shenkman
authors.forename: Z A
authors.initials: ZA
authors.lastname: Podlubnaia
authors.forename: I M
authors.initials: IM
authors.lastname: Vikhliantsev
authors.forename: K S
authors.initials: KS
authors.lastname: Litvinova
authors.forename: S N
authors.initials: SN
authors.lastname: Udal'tsov
authors.forename: T L
authors.initials: TL
authors.lastname: Nemirovskaia
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Lemesheva
authors.forename: A M
authors.initials: AM
authors.lastname: Mukhina
authors.forename: I B
authors.initials: IB
authors.lastname: Kozlovskaia
mesh_terms: D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007537:Isometric Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000276:immunology / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000032:analysis / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism*; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: D003598:Cytoskeletal Proteins; D018995:Myosin Heavy Chains