title: Electron beam-induced graft polymerization of acrylic acid and immobilization of arginine-glycine-aspartic acid-containing peptide onto nanopatterned polycaprolactone.
abstract: Electron beam- (EB-) induced graft polymerization of acrylic acid and the subsequent immobilization of arginine-glycine-aspartic acid (RGD) peptide onto nanopatterned polycaprolactone with parallel grooves is reported. A high concentration of carboxylic groups was introduced onto the polymer substrate by EB-induced polymerization of acrylic acid. In the coupling of the RGD peptide to the carboxylated polymer surface, a three-step peptide immobilization process was used. This process included the activation of surface carboxylic acid into an active ester intermediate by use of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), the introduction of disulfide groups by use of 2-(2-pyridinyldithio)ethanamine hydrochloride (PDEA), and final immobilization of the peptide via a thiol-disulfide exchange reaction. The extent of coupling was measured by UV spectroscopy. A preliminary study of the in vitro behavior of keratinocytes (NCTC 2544) cultured on the acrylic acid-grafted and RGD peptide-coupled surface showed that most cells grown on the coupled samples had a spread-rounded appearance, while the majority of cells tended to be elongated along the grooves on uncoupled substrates.
authors.affiliation: Department of Fiber and Polymer Technology, Royal Institute of Technology (KTH), Teknikringen 56-58, S 100 44 Stockholm, Sweden.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Anders
authors.initials: A
authors.lastname: Wirsén
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Albertsson
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004583:Electrons; D015603:Keratinocytes / Q000648:ultrastructure; D008024:Ligands; D046911:Macromolecular Substances / Q000737:chemistry*; D008956:Models, Chemical; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; D001672:Biocompatible Materials; D004220:Disulfides; D008024:Ligands; D046911:Macromolecular Substances; D009842:Oligopeptides; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D013438:Sulfhydryl Compounds; C016240:polycaprolactone; C006903:carbopol 940; C047981:arginyl-glycyl-aspartic acid; C036658:acrylic acid