A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
Recently several strategies to treat ischemic diseases have been proposed but the ideal way has to be determined. We explored whether human placenta-derived mesenchymal cells (hPDMCs) can be used for this purpose because placenta is very rich in vessels. First, production of human vascular endothelial growth factor (hVEGF) from hPDMCs was examined. The amount of hVEGF secreted by hPDMCs was similar to the amount produced by HeLa cells. hVEGF was barely detected in human umbilical vein endothelial cells (hUVECs) or human peripheral blood mononuclear cells. hVEGF secreted from hPDMCs stimulated the proliferation of hUVECs, indicating its biological activity. Transplantation of hPDMCs to the ischemic limbs of NOD/Shi-scid mice significantly improved the blood flow of the affected limbs. Blood vessel formation was more prominently observed in the limbs of treated mice as compared to the control mice. Real-time RT-PCR revealed that hPDMCs produced hVEGF for at least 7 days after transplantation. Thus, transplantation of hPDMCs could potentially be a promising treatment for human ischemic diseases.
Nishishita
Toshihide
T
Department of Advanced Medical Science, Institute of Medical Science, University of Tokyo, Tokyo, Japan. tnishi-tky@umin.ac.jp
Ouchi
Kunie
K
Zhang
Xiaohong
X
Inoue
Mariko
M
Inazawa
Takeshi
T
Yoshiura
Kenta
K
Kuwabara
Koichiro
K
Nakaoka
Takashi
T
Watanabe
Nobukazu
N
Igura
Koichi
K
Takahashi
Tsuneo A
TA
Yamashita
Naohide
N
D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000254:growth & development; D017690:Cell Transplantation; D002478:Cells, Cultured; D005260:Female; D006367:HeLa Cells; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D042461:Vascular Endothelial Growth Factor A