Improved separation of metallothionein isoforms by the presence of cyclodextrin in capillary zone electrophoresis.
Capillary zone electrophoresis (CZE) with cyclodextrin (CD) in the polyacrylamide-coated capillary was used to study metallothionein (MT) forms in the horse kidney preparation produced commercially by Sigma. It is known that CDs form complexes with hydrophobic amino acids. The results show that the presence of CD improves the separability of the various MT forms, including the MT-IA and the MT-IB forms, metallothionein aggregates, as well as the so far unidentified a and b forms. This was true both below and above the isoelectric points (pIs), although the migration times were somewhat longer at increasing CD concentrations for runs at constant voltage than with constant current.
Wilhelmsen
Thore W
TW
Pharmaceutical Department, Norwegian Medicines Agency, Oslo, Norway. Norway.
Hansen
Bjørn Henrik
BH
Holten
Valborg
V
Olsvik
Pal A
PA
Andersen
Rolf A
RA
D000818:Animals; D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006736:Horses; D007668:Kidney / Q000737:chemistry; D008668:Metallothionein / Q000302:isolation & purification*; D020033:Protein Isoforms / Q000302:isolation & purification*
D003505:Cyclodextrins; D020033:Protein Isoforms; D008668:Metallothionein