title: A rapid, sensitive and validated method for the determination of ondansetron in human plasma by reversed-phase high-pressure liquid chromatography.
abstract: A simple and sensitive method for the determination of ondansetron (CAS 116002-70-1) in human plasma was developed using high-pressure liquid chromatography (HPLC). The procedure involves extraction of human plasma with tertiary butyl methyl ether containing 2 mol/l sodium hydroxide, followed by reversed-phase HPLC using a LiChrospher 100 RP-18e 5 microm column and UV detection at 305 nm. The retention times of ondansetron and internal standard (propranolol hydrochloride, CAS 318-98-9) were 9.38 and 13.40 min, respectively. The calibration curves were linear over the range of 10 ng/ml (lower limit of quantitation, LOQ) and 380 ng/ml for ondansetron. The intra- and inter-assay coefficients of variation for all the criteria of validation were less than 15% over the linearity range. Ondansetron was stable upon storage in human plasma. The sensitivity and precision of the method were within the accepted limits (< 15 %) throughout the validation period. The present method is useful for determination of plasma concentrations of ondansetron during human pharmacokinetic studies.
authors.affiliation: Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh (India).
authors.forename: Durairaj
authors.initials: D
authors.lastname: Chandrasekar
authors.forename: Sistla
authors.initials: S
authors.lastname: Ramakrishna
authors.forename: Prakash V
authors.initials: PV
authors.lastname: Diwan
mesh_terms: D000932:Antiemetics / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005615:Freezing; D006801:Humans; D017294:Ondansetron / Q000097:blood*; D011786:Quality Control; D015203:Reproducibility of Results; D013048:Specimen Handling; D013056:Spectrophotometry, Ultraviolet; D013810:Therapeutic Equivalency
chemical_list: D000932:Antiemetics; D017294:Ondansetron