title: Effects of endocrine-disrupting chemicals on expression of ubiquitin C-terminal hydrolase mRNA in testis and brain of the Japanese common goby.
abstract: We investigated the effects of endocrine-disrupting chemicals (EDCs) on the expression of ubiquitin C-terminal hydrolase (UCH) mRNA in the testis and brain of the Japanese common goby, Acanthogobius flavimanus. The cDNA sequence of goby UCH contained an open reading frame encoding 220 amino acid residues (M(r)=24,223) with 51.3% overall sequence identity with human and mouse UCHL1. A competitive PCR assay was used to quantify the levels of UCH mRNA in the testis and brain of male gobies after exposure to bisphenol A, nonylphenol, or estradiol-17beta for 3 weeks. Exposure to estradiol-17beta at a nominal concentration of 100 ng/L induced significant increase in UCH mRNA levels in both testis and brain (P<0.05), whereas exposure to nonylphenol induced a significant decrease in UCH mRNA levels in the testis (P<0.01). These results suggest that EDCs can either positively or negatively regulate UCH mRNA levels.
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Maruishi 2-17-5, Ohno, Saeki, Hiroshima 739-0452, Japan. kmochida@fra.affrc.go.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Mochida
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ohkubo
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsubara
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ito
authors.forename: Akira
authors.initials: A
authors.lastname: Kakuno
authors.forename: Kazunori
authors.initials: K
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D000726:Androgen Antagonists / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D001559:Benzhydryl Compounds; D015152:Blotting, Northern; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004958:Estradiol / Q000633:toxicity; D004965:Estrogen Antagonists / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism; D010636:Phenols / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013737:Testis / Q000187:drug effects / Q000201:enzymology; D043222:Ubiquitin Thiolesterase / Q000096:biosynthesis / Q000235:genetics*; D014819:Vitellogenins / Q000097:blood; D000098826:Bisphenol A Compounds
chemical_list: D000726:Androgen Antagonists; D001559:Benzhydryl Compounds; D004958:Estradiol; D004965:Estrogen Antagonists; D010636:Phenols; D012333:RNA, Messenger; D043222:Ubiquitin Thiolesterase; D014819:Vitellogenins; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds