Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice.
In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-y, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4+ T cells were the major source of IFN-y in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.
Pereira
V R A
VR
Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil.
Lorena
V M B
VM
Da Silva
A P Galvao
AP
Coutinho
E M
EM
Silvas
E D
ED
Ferreira
A G P
AG
Miranda
P
P
Krieger
M A
MA
Goldenberg
S
S
Soares
M B P
MB
Correa-Oliveira
R
R
Gomes
Y M
YM
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D005434:Flow Cytometry; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015800:Protozoan Proteins / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D016207:Cytokines; D015800:Protozoan Proteins; C060967:cytoplasmic antigen, Trypanosoma; C052356:flagellum-specific antigen, Trypanosoma