title: A liquid chromatographic-tandem mass spectrometric method for the determination of YM466, a novel Factor Xa inhibitor, in rat plasma.
abstract: A sensitive and selective method for the determination of YM466, a novel Factor Xa inhibitor, in rat plasma was developed and validated using liquid chromatography-mass spectrometry with electrospray ionization. Plasma samples were pretreated by solid-phase extraction and chromatographed on a C18 column (75 mm x 4.6 mm i.d.) with a mobile phase consisting of 0.1% formic acid-methanol (70:30 (v/v)), and detected using selected reaction monitoring in the positive-ion mode. The lower limit of quantification was 0.4 ng/ml, and good precision and accuracy were achieved. The validated method allowed analysis of samples for the determination of pharmacokinetic profiles of YM466 in rats.
authors.affiliation: Drug Metabolism Laboratories, Yamanouchi Pharmaceutical Co. Ltd., 1-8 Azusawa 1-Chome, Itabashi-ku, Tokyo 174-8511, Japan. mano@yamanouchi.co.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mano
authors.forename: Takashi
authors.initials: T
authors.lastname: Usui
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Kamimura
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D009281:Naphthalenes / Q000097:blood* / Q000737:chemistry; D010880:Piperidines / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015842:Serine Proteinase Inhibitors / Q000032:analysis / Q000737:chemistry
chemical_list: D009281:Naphthalenes; D010880:Piperidines; D015842:Serine Proteinase Inhibitors; C111032:YM 60828; D000990:Antithrombin III