Capacity of Bacillus thuringiensis S-layer protein displaying polyhistidine peptides on the cell surface.
S-layer protein of Bacillus thuringiensis strain CTC was used as the carrier protein to display polyhistidine (poly[6His]) peptides on the cell surface. Poly(6His)n was fused with S-layer protein at two different sites, inserting just downstream of the S-layer protein homologous domain (slh) and replacing the non-slh region of S-layer protein, respectively. The two series chimeric proteins were both expressed by crystal negative B. thuringiensis strain 4Q7 and strain 171, respectively, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The recombinant B. thuringiensis cells gained Ni(2+)- and Cd(2+)-binding ability and had a capacity to display up to nine copies of poly(6His). The Cd(2+) adsorption quantity of the recombinant strain with the strongest adsorption ability was twice that of the host strain.
Wang
Li
L
College of Life Science and Technology, Huazhong Agricultural University, State-Key Laboratory of Agricultural Microbiology, Wuhan 430070, People's Republic of China.
Sun
Ming
M
Yu
Ziniu
Z
D001413:Bacillus thuringiensis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D005240:Feasibility Studies; D005786:Gene Expression Regulation / Q000502:physiology; D006639:Histidine / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; C033223:polyhistidine; D006639:Histidine