title: The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase.
abstract: The S. pombe Cdc14-related phosphatase Clp1p/Flp1p regulates G2/M transition by antagonizing CDK activity and is essential for coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. At the G2/M transition, Clp1p/Flp1p is released from the nucleolus and SPB and distributes throughout the nucleus to the spindle and the contractile ring. This early relocalization is analogous to vertebrate Cdc14 homologs and stands in contrast to S. cerevisiae Cdc14p, which is not released from the nucleolus until metaphase/anaphase transition. Here, we report that Clp1p/Flp1p localizes to kinetochores in prometaphase and functions in chromosome segregation, since deletion of clp1/flp1 causes cosegregation of sister chromatids, when sister kinetochores are prone to mono-orientation. Genetic, cytological, and biochemical experiments suggest that Clp1p/Flp1p functions together with Aurora kinase at kinetochores. Together, these results suggest that Clp1p/Flp1p has a role in repairing mono-orientation of sister kinetochores.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, MA 01605, USA.
authors.forename: Susanne
authors.initials: S
authors.lastname: Trautmann
authors.forename: Srividya
authors.initials: S
authors.lastname: Rajagopalan
authors.forename: Dannel
authors.initials: D
authors.lastname: McCollum
mesh_terms: D064127:Aurora Kinases; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism; D002842:Chromatids; D002875:Chromosomes / Q000502:physiology*; D050759:Cyclin-Dependent Kinase Inhibitor p21; D048749:Cytokinesis; D005456:Fluorescent Dyes; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D018386:Kinetochores / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D011233:Precipitin Tests; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D013388:Succinimides
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; C417664:Alexa594; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013388:Succinimides; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases