Analysis of the temporal events in blood and tissues following fecal peritonitis in mice.
BACKGROUND
The kinetics of systemic responses triggered by bacterial peritonitis were analysed in the blood, bronchoalveolar lavage (BAL) and several organs.


METHODS
The murine model of cecal ligation and puncture was employed. The parameters were analysed at different periods of time (4 to 72 h).


RESULTS
Fecal peritonitis triggered a progressive, but not significant, decline of blood leukocytes between 4 and 6 h, becoming significant at 24 to 48 h (p<0.05). This profile was closely associated with the enhancement of leukocytes both in the abdomen (p<0.05) and in the BAL (p<0.05). A significant abdominal exudation was detected between 4 and 72 h (p<0.01), whereas maximal growth of aerobic bacteria in the blood and lungs was observed 24 and 72 h after. Maximal exudation in the studied tissues occurred at different time points (heart=24 h, spleen and kidney=48 h, liver and lung= 72 h).


CONCLUSIONS
Using this model, evidence of sepsis can be easily measured in different body systems.
Santos
Vanessa V
VV
Departments of Clinical Analysis and Pharmacology, Centre of Health, Universidade Federal de Santa Catarina 88040-970, Florianópolis, SC, Brazil.
Fröde
Tania S
TS
Medeiros
Yara S
YS
D000818:Animals; D001420:Bacteria, Aerobic; D001424:Bacterial Infections / Q000097:blood* / Q000382:microbiology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000382:microbiology; D002465:Cell Movement; D003106:Colon / Q000293:injuries; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D007700:Kinetics; D007958:Leukocyte Count; D007962:Leukocytes / Q000382:microbiology / Q000473:pathology*; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000097:blood* / Q000209:etiology / Q000503:physiopathology; D012772:Shock, Septic / Q000097:blood* / Q000209:etiology / Q000473:pathology; D013997:Time Factors