title: Structure-activity relationship of lysophosphatidylcholines in HL-60 human leukemia cells.
abstract: OBJECTIVE
  To explore the structure-activity relationship of lysophosphatidylcholine (LPC) and lysolipid molecules from a marine sponge and ladybirds.
  
  
  METHODS
  We tested three synthetic LPCs and four natural lysolipids on Ca2+ mobilization in HL-60 human leukemia cells.
  
  
  RESULTS
  We observed lysolipid-mediated Ca2+ mobilization. The activity was the same in both ester- and ether-linked lysolipids, and introduction of a double bond or methoxy group on the alkyl chain did not significantly modulate the activity. However, replacement of trimethylammonium moiety in the choline structure with ammonium moiety reduced the activity. Furthermore, change of the alkyl chain length influenced the Ca2+ response.
  
  
  CONCLUSIONS
  LPC-induced Ca2+ mobilization might be dependent on the length of alkyl chain and the presence of choline moiety in HL-60 leukemia cells.
authors.affiliation: Laboratory of Pharmacology, College of Pharmacy, Pusan National University, Busan, Korea.
authors.forename: Eun-hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Mi-ran
authors.initials: MR
authors.lastname: Yun
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Wang
authors.forename: Jee H
authors.initials: JH
authors.lastname: Jung
authors.forename: Dong-soon
authors.initials: DS
authors.lastname: Im
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D001517:Coleoptera / Q000737:chemistry; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D008244:Lysophosphatidylcholines; D002118:Calcium