Identification of a novel target of D/V signaling in Drosophila wing disc: Wg-independent function of the organizer.
Growth and patterning during Drosophila wing development are mediated by signaling from its dorso-ventral (D/V) organizer. Wingless is expressed in the D/V boundary and functions as a morphogen to activate target genes at a distance. Wingless pathway and thereby D/V signaling is negatively regulated by the homeotic gene Ultrabithorax (Ubx) to mediate haltere development. In an enhancer-trap screen to identify genes that show differential expression between wing and haltere discs, we identified CG32062, which codes for a RNA-binding protein. In wing discs, CG32062 is expressed only in non-D/V cells. CG32062 expression in non-D/V cells is dependent on Notch-mediated signaling from the D/V boundary. However, CG32062 expression is independent of Wingless function, thus providing evidence for a second long-range signaling mechanism of the D/V organizer. In haltere discs, CG32062 is negatively regulated by Ubx. The non-cell autonomous nature of Ubx-mediated repression of CG32062 expression suggests that the novel component of D/V signaling is also negatively regulated during haltere specification.
Bajpai
Ruchi
R
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India 500 007.
Sambrani
Nagraj
N
Stadelmayer
Bernd
B
Shashidhara
L S
LS
D000818:Animals; D015415:Biomarkers; D019521:Body Patterning / Q000502:physiology*; D004330:Drosophila / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D020897:Organizers, Embryonic / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000254:growth & development* / Q000378:metabolism; D051155:Wnt1 Protein
D015415:Biomarkers; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D011518:Proto-Oncogene Proteins; D016601:RNA-Binding Proteins; C500367:Rbfox1 protein, Drosophila; D014157:Transcription Factors; C066177:Ubx protein, Drosophila; D051155:Wnt1 Protein; C074622:wg protein, Drosophila