title: Identification of mutations associated with pyrethroid resistance in the para-type sodium channel of the cat flea, Ctenocephalides felis.
abstract: Knockdown resistance (kdr) to pyrethroid insecticides is caused by point mutations in the pyrethroid target site, the para-type sodium channel of nerve membranes. This most commonly involves alterations within the domain II (S4-S6) region of the channel protein where five different mutation sites have been identified across a range of insect species. To investigate the incidence of this mechanism in cat fleas, we have cloned and sequenced the IIS4-IIS6 region of the para sodium channel gene from seven laboratory flea strains. Analysis of these sequences revealed two amino acid replacements at residues previously implicated in pyrethroid resistance. One is the 'common' kdr mutation, a leucine to phenylalanine substitution (equivalent to L1014F of housefly) reported previously in several other insects. The other is a threonine to valine substitution (equivalent to T929V) and is a novel variant of the T929I mutation first identified in diamondback moth. The L1014F mutation was found at varying frequency in all of the laboratory flea strains, whereas the T929V mutation was found only in the highly resistant Cottontail strain. We have developed rapid PCR-based diagnostic assays for the detection of these mutations in individual cat fleas and used them to show that both L1014F and T929V are common in UK and US flea populations. This survey revealed a significant number of fleas that carry only the V929 allele indicating that co-expression with the F1014 allele is not necessary for flea viability.
authors.affiliation: Department of Biological Chemistry, Rothamsted Research, St Albans Road, Harpenden AL5 2JQ, UK. martin.williamson@bbsrc.ac.uk
authors.forename: Chris
authors.initials: C
authors.lastname: Bass
authors.forename: Iris
authors.initials: I
authors.lastname: Schroeder
authors.forename: Andreas
authors.initials: A
authors.lastname: Turberg
authors.forename: Linda
authors.initials: L
authors.lastname: M Field
authors.forename: Martin S
authors.initials: MS
authors.lastname: Williamson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000469:parasitology; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D007305:Insecticide Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011722:Pyrethrins / Q000494:pharmacology*; D005423:Siphonaptera / Q000235:genetics* / Q000502:physiology; D015222:Sodium Channels / Q000235:genetics*
chemical_list: D011722:Pyrethrins; D015222:Sodium Channels