title: The effect of tryptophan depletion on brain activation measured by functional magnetic resonance imaging during the Stroop test in healthy subjects.
abstract: We investigated the role of serotonin in cognitive activation of the frontal cortex. The serotonergic system was affected by the administration of an amino acids mixture without tryptophan (tryptophan depletion). In a placebo-controlled double-blind cross-over study with 20 healthy volunteers, we tested the hypothesis that a tryptophan (serotonin) decrease affects the activation of prefrontal cortex by the Stroop test. Cognitive brain activation was evaluated by functional magnetic resonance imaging (fMRI). Tryptophan depletion decreased the plasma tryptophan level up to 90 % for five hours after the tryptophan-free drink had been consumed when compared with the same mixture with tryptophan (p?0.0001). Tryptophan depletion did not affect the Stroop test performance. We compared fMRI activation in both conditions (tryptophan depletion and placebo) with plasma tryptophan levels as the covariates. The tryptophan depletion increased the activation (fMRI signal) in the bilateral mediofrontal cortex, anterior cingulate and left dorsolateral prefrontal cortex. The present findings allow the postulate that serotonergic medial forebrain and cingulum bundle pathways play a role in the activity of cortical structures involved in Stroop test processing.
authors.affiliation: Prague Psychiatric Centre, Ustavni 91, 18100 Prague 8, Czech Republic. horacek@pcp.lf3.cuni.cz
authors.forename: J
authors.initials: J
authors.lastname: Horácek
authors.forename: L
authors.initials: L
authors.lastname: Závesická
authors.forename: J
authors.initials: J
authors.lastname: Tintera
authors.forename: C
authors.initials: C
authors.lastname: Dockery
authors.forename: V
authors.initials: V
authors.lastname: Platilová
authors.forename: M
authors.initials: M
authors.lastname: Kopecek
authors.forename: F
authors.initials: F
authors.lastname: Spaniel
authors.forename: V
authors.initials: V
authors.lastname: Bubeníková
authors.forename: C
authors.initials: C
authors.lastname: Höschl
mesh_terms: D000328:Adult; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D011597:Psychomotor Performance / Q000187:drug effects / Q000502:physiology*; D018709:Statistics, Nonparametric; D014364:Tryptophan / Q000008:administration & dosage / Q000172:deficiency*
chemical_list: D014364:Tryptophan