title: Simultaneous HPLC determination of ketoprofen and its degradation products in the presence of preservatives in pharmaceuticals.
abstract: A novel and quick high-performance liquid chromatography (HPLC) method with UV spectrophotometric detection was developed and validated for the determination of five compounds in topical gel. The described method is suitable for simultaneous determination of active component ketoprofen, two preservatives methylparaben and propylparaben and two degradation products of ketoprofen--3-acetylbenzophenone and 2-(3-carboxyphenyl) propionic acid--in a topical cream after long-term stability tests using ethylparaben as an internal standard. The chromatographic separation was performed on a 5microm Supelco Discovery C18 column (125mm x 4mm i.d., Sigma-Aldrich); the optimal mobile phase for separation of ketoprofen, methylparaben, propylparaben, degradation products 3-acetylbenzophenone and 2-(3-carboxyphenyl) propionic acid and ethylparaben as internal standard consists of a mixture of acetonitril, water and phosphate buffer pH 3.5 (40:58:2, v/v/v). At a flow rate of 1.0ml min(-1) and detection at 233nm, the total time of analysis was less than 10min. The method was applied for routine analysis (batch analysis and stability tests) of these compounds in topical pharmaceutical product.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Dvorák
authors.forename: R
authors.initials: R
authors.lastname: Hájková
authors.forename: L
authors.initials: L
authors.lastname: Matysová
authors.forename: L
authors.initials: L
authors.lastname: Nováková
authors.forename: M A
authors.initials: MA
authors.lastname: Koupparis
authors.forename: P
authors.initials: P
authors.lastname: Solich
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007660:Ketoprofen / Q000032:analysis* / Q000378:metabolism*; D011310:Preservatives, Pharmaceutical / Q000032:analysis* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D011310:Preservatives, Pharmaceutical; D007660:Ketoprofen