Site-selective DNA photocleavage involving unusual photoinitiated tautomerization of chiral tridentate vanadyl(V) complexes derived from N-salicylidene alpha-amino acids.
The titled vanadyl(V) complexes serve as efficient reagents for cleaving supercoiled plasmid DNA by photoinitiation. Complex 3d, derived from 2-hydroxy-1-naphthaldehyde and l-phenylalanine, exhibits a unique wedge feature, inducing a site-selective photocleavage at the C22-T23 of the bulge backbone for a HIV-27 DNA system at 0.1-5 muM. Transient absorption experiments for 3d indicate the involvement of LMCT with concomitant tautomerization, leading to an o-quinone-methide V-bound hydroxyl species responsible for the cleavage profiles. [structure: see text]
Chen
Chien-Tien
CT
Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan. chefv43@scc.ntnu.edu.tw
Lin
Jin-Sheng
JS
Kuo
Jen-Huang
JH
Weng
Shiue-Shien
SS
Cuo
Ting-Shen
TS
Lin
Yi-Wen
YW
Cheng
Chien-Chung
CC
Huang
Yan-Chen
YC
Yu
Jen-Kan
JK
Chou
Pi-Tai
PT
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D016325:HIV Long Terminal Repeat; D008958:Models, Molecular; D010782:Photolysis; D010957:Plasmids / Q000737:chemistry; D017968:Vanadium Compounds / Q000737:chemistry*
D017968:Vanadium Compounds; D004247:DNA