Usefulness of combined continuous suture and interrupted tie technique in hindlimb composite tissue allotransplantation in rats.
According to our experience of over 100 hindlimb transplantations, in rats, catching the posterior wall with an anterior wall suture is the most common cause of thrombosis and acute transplant failure. It is generally due to inadequate visibility of the lumen and consequent blind suture placement. With an intention to find a superior suture technique, a comparative study between standard end-to-end interrupted and combined continuous sutures and interrupted tie techniques was performed. Twenty hindlimb transplantations to rats' femoral vessels were performed in each group. Anastomosis time, patency, complications, and animal and transplant survivals were compared. In the interrupted suture group (group I), anastomosis failure was encountered in one vein, with 95% of immediate patency rate. A redo of the venous anastomosis was performed and successful. The combined suture technique (group II) had 100% patency rates without complications. The average time for arterial anastomosis in groups I and II was 8.5 and 8.9 min, and for venous anastomosis, 10.7 and 9.6 min, respectively. At 2 weeks, no differences were found between groups as related to transplant and animal survival.
Ulusal
Betul Gozel
BG
Department of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College, Chang Gung University, Taipei, Taiwan.
Ulusal
Ali Engin
AE
Hung
Li-Man
LM
Wei
Fu Chan
FC
D000714:Anastomosis, Surgical; D000818:Animals; D005268:Femoral Vein / Q000601:surgery*; D008866:Microsurgery; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014654:Vascular Patency