Transmission of prions from mule deer and elk with chronic wasting disease to transgenic mice expressing cervid PrP.
We generated mice expressing cervid prion protein to produce a transgenic system simulating chronic wasting disease (CWD) in deer and elk. While normal mice were resistant to CWD, these transgenic mice uniformly developed signs of neurological dysfunction approximately 230 days following intracerebral inoculation with four CWD isolates. Inoculated transgenic mice homozygous for the transgene array developed disease after approximately 160 days. The brains of sick transgenic mice exhibited widespread spongiform degeneration and contained abnormal prion protein and abundant amyloid plaques, many of which were florid plaques. Transmission studies indicated that the same prion strain caused CWD in the analyzed mule deer and elk. These mice provide a new and reliable tool for detecting CWD prions.
Browning
Shawn R
SR
Department of Microbiology, University of Kentucky, 800 Rose St., Lexington, KY 40536, USA.
Mason
Gary L
GL
Seward
Tanya
T
Green
Mike
M
Eliason
Gwyneth A J
GA
Mathiason
Candace
C
Miller
Michael W
MW
Williams
Elizabeth S
ES
Hoover
Ed
E
Telling
Glenn C
GC
D000818:Animals; D003670:Deer; D051379:Mice; D008822:Mice, Transgenic; D018620:PrPSc Proteins / Q000378:metabolism; D011328:Prions / Q000502:physiology*; D034081:Wasting Disease, Chronic / Q000209:etiology / Q000473:pathology / Q000635:transmission*
D018620:PrPSc Proteins; D011328:Prions