title: The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary.
abstract: OBJECTIVE
  This experimental study was designed to determine the changes in tissue levels of malondialdehyde, end-product of lipid peroxidation (MDA), reduced glutathione (GSH) and xanthine oxidase (XO) and the effect of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE) on these metabolite levels after adnexal torsion-detorsion model in rats.
  
  
  METHODS
  Forty adult female albino rats were divided into five groups: basal control (n = 8), sham operation (n = 8), torsion-detorsion plus saline (n = 8), torsion-detorsion plus CAPE (n = 8). and only torsion (n = 8). Rats in the sham operation group underwent a surgical procedure similar to the other groups but the adnexa was not torsioned. Rats in the torsion group were killed after 360 degrees clockwise adnexal torsion for 3 h and ovaries were harvested. CAPE was injected intraperitoneally 30 min before detorsion in the CAPE/detorsion group and saline was administered in the saline/detorsion group. After 3 h of adnexal detorsion, the rats in both groups were killed and adnexa were surgically removed.
  
  
  RESULTS
  MDA levels and XO activities in torsion-detorsion plus saline group increased significantly when compared to basal control, torsion and sham operation groups (P < 0.001). In the CAPE group, MDA levels and XO activities were lower than those of torsion-detorsion plus saline group, and differences between the two groups were statistically significant (P < 0.001). GSH levels in torsion-detorsion plus saline group were decreased significantly when compared to basal control and sham operation groups (P < 0.001). GSH levels in the CAPE group were higher than those of torsion-detorsion plus saline group, and differences between the two groups were statistically significant (P < 0.004). Morphologically, polymorphonuclear leukocytic infiltration and vascular dilatation were obvious in the ischemia-reperfusion damaged ovary, a change partially reversed by CAPE.
  
  
  CONCLUSIONS
  These results suggest that administration of CAPE has beneficial effects in the prevention of ischemia-reperfusion injury of the ovaries.
authors.affiliation: Department of Obstetrics and Gynecology, Inonu University Medical Faculty, 44069, Malatya, Turkey. oncelik@inonu.edu.tr
authors.forename: Onder
authors.initials: O
authors.lastname: Celik
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Turkoz
authors.forename: Seyma
authors.initials: S
authors.lastname: Hascalik
authors.forename: Mehmet
authors.initials: M
authors.lastname: Hascalik
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Cigremis
authors.forename: Bulent
authors.initials: B
authors.lastname: Mizrak
authors.forename: Saim
authors.initials: S
authors.lastname: Yologlu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002109:Caffeic Acids / Q000627:therapeutic use*; D005260:Female; D015227:Lipid Peroxidation; D023421:Models, Animal; D010049:Ovarian Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol