title: Optical biopsy of liver fibrosis by use of multiphoton microscopy.
abstract: We demonstrate the application of multiphoton microscopy in diagnosing toxin- (CCl4-) induced liver fibrosis in mice. Although hepatocyte autofluorescence does not vary significantly, different degrees of necrosis and stellate cell proliferation at necrotic sites in livers with fibrosis (ex vivo) can be detected easily from multiphoton-induced autofluorescence images by use of 780-nm excitation. Our result suggests that multiphoton microscopy can be developed into an effective technique for the detection and diagnosis of liver fibrosis in vivo.
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
authors.forename: Hsuan-Shu
authors.initials: HS
authors.lastname: Lee
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hsiao-Ching
authors.initials: HC
authors.lastname: Chen
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Chiou
authors.forename: Guan-Tarn
authors.initials: GT
authors.lastname: Huang
authors.forename: Wen
authors.initials: W
authors.lastname: Lo
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000818:Animals; D001706:Biopsy / Q000295:instrumentation / Q000379:methods; D002251:Carbon Tetrachloride; D022781:Hepatocytes / Q000473:pathology*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*
chemical_list: D002251:Carbon Tetrachloride