title: Marrow-derived osteoblasts seeded into porous natural coral to prefabricate a vascularised bone graft in the shape of a human mandibular ramus: experimental study in rabbits.
abstract: To find out if it was possible to prefabricate bone graft in the shape of a human mandibular ramus that possessed a pedicle that carried blood. The pore size of the natural coral was about 200 microm with a porosity of about 36%. The natural coral was made into the shape of a human mandibular ramus. Marrow-derived osteoblasts were seeded into porous natural coral scaffolds in a density of 2 x 10(8)/mL in 300 microL cell suspension. After two days of in vitro incubation, five cell-coral complexes were implanted into cell donor rabbits under the inferior epigastric blood vessels to prefabricate a vascularised bone graft of specific shape. Two months later the bone formation was observed by gross inspection and histological examination. Two months after operation, a well-vascularised bone graft in the shape of the initial coral scaffold and with a blood-carrying pedicle had been regenerated successfully. Osteogenesis followed the pattern of endochondral bone formation. New bone could be seen on the surface and in the pores of coral on histological examination. We have shown that it is possible to fabricate vascularised bone graft in a predetermined shape using tissue engineering. This kind of bone graft may have future clinical application.
authors.affiliation: Department of Oral Maxillofacial Surgery, Qin Du Hospital, School of Stomatology, The Fourth Military Medical University, Kang Fu Road 7, Xi'an 710032, PR China. cflfx@163.net
authors.forename: Fulin
authors.initials: F
authors.lastname: Chen
authors.forename: Shujun
authors.initials: S
authors.lastname: Chen
authors.forename: Kai
authors.initials: K
authors.lastname: Tao
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.forename: Yanpu
authors.initials: Y
authors.lastname: Liu
authors.forename: Delin
authors.initials: D
authors.lastname: Lei
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Mao
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000098:blood supply; D002119:Calcium Carbonate; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003063:Cnidaria; D006801:Humans; D008334:Mandible / Q000166:cytology; D010006:Osteoblasts; D010012:Osteogenesis; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D002119:Calcium Carbonate