title: Expression of kinin B1 receptors in the spinal cord of streptozotocin-diabetic rat.
abstract: Previous studies have reported cardiovascular and nociceptive responses after intrathecal injection of kinin B1 receptor (B1R) agonists in the model of streptozotocin (STZ)-diabetic rat (diabetic). The aim of this study was to measure the early up-regulation of B1R binding sites and mRNA in the thoracic spinal cord of diabetic and control rats. Data show significant increases of specific B1R binding sites in the dorsal horn of diabetic rats 2 days (+315%), 7 days (+303%) and 21 days (+181%) after STZ treatment. Levels of mRNA were significantly increased (+68%) at 2 and 7 days but not at 21 days. These data bring the first molecular evidence for an early up-regulation of B1R in the spinal cord of diabetic rat.
authors.affiliation: Department of Physiology, Université de Montréal, Pavillon Paul-G.-Desmarais, 2960, Chemin de la Tour, Montréal, Québec, H3T 1J4, Canada.
authors.forename: Brice
authors.initials: B
authors.lastname: Ongali
authors.forename: Maria Martha
authors.initials: MM
authors.lastname: Campos
authors.forename: Mihai
authors.initials: M
authors.lastname: Petcu
authors.forename: Donata
authors.initials: D
authors.lastname: Rodi
authors.forename: Frank
authors.initials: F
authors.lastname: Cloutier
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: Chabot
authors.forename: Gaétan
authors.initials: G
authors.lastname: Thibault
authors.forename: Réjean
authors.initials: R
authors.lastname: Couture
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization / Q000379:methods; D007456:Iodine Isotopes / Q000493:pharmacokinetics; D008297:Male; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D011859:Radiography / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D043783:Receptor, Bradykinin B1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013116:Spinal Cord / Q000378:metabolism*; D044005:Tetrahydroisoquinolines / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007456:Iodine Isotopes; D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D044005:Tetrahydroisoquinolines; C078665:HOE 140, desArg(10)-; D001920:Bradykinin