title: Efficient plant regeneration from suspension cells of Allium cepa L.
abstract: Plant regeneration from calli of three cultivars of Allium cepa (Senshuki, O.Pki and Shojovaka) was investigated. Callus was induced on four variations of BDS medium containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BAP). The regeneration frequency of calli of cvs. Senshuki and O.Pki subcultured on solid MS medium supplemented with BAP ranged from 50% to 80%; this frequency decreased to less than 30% after subculture in the dark in liquid BDS medium. By repeating the dark/light transitions of the culture protocol and by selecting for green cell clusters, we were able to increase the regeneration frequency to more than 80% in all three cultivars. These cell clusters maintained a high regeneration capacity in subsequent subcultures in the absence of light for 2 months. Most (97%) of the regenerated plantlets had a normal diploid karyotype (2 n=16) that was identical to that of the mother plants, although 3% of the regenerated plants of cv. Shojovaka had a tetraploid karyotype.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto, 860-8555, Japan.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Lin
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Takano
authors.forename: Susumu
authors.initials: S
authors.lastname: Takio
authors.forename: Kanji
authors.initials: K
authors.lastname: Ono
mesh_terms: D018929:Cell Culture Techniques; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008027:Light; D019697:Onions / Q000166:cytology* / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D011003:Ploidies; D012038:Regeneration
chemical_list: D003470:Culture Media