title: Comparison of different attenuation strategies in development of a Salmonella hadar vaccine.
abstract: The purpose of this work was to develop a live, attenuated vaccine strain to protect chickens against colonization by group C Salmonella. We constructed two candidate vaccines: a deltacya deltacrp derivative and a deltaphoP derivative of Salmonella hadar. White Leghorn chickens were vaccinated at day of age and at 2 wk with one of the two strains. A nonvaccinated group served as a control. At 4 wk of age, all birds were challenged with wild-type S. hadar and necropsied 6 days later. Numbers of S. hadar in the ceca were determined. Enzyme-linked immunosorbent assay-derived serum immunoglobulin G responses against S. hadar lipopolysaccharide indicated that both strains induced a serum antibody response. The average optical density450 for birds vaccinated with the deltaphoP or deltacya deltacrp derivatives was 0.456 and 0.881, respectively. Although the deltacya deltacrp derivative induced higher levels of serum antibody, it did not provide an immune response protective against colonization by S. hadar. Conversely, birds vaccinated with the deltaphoP strain showed significant protection against S. hadar challenge. Seventy percent of the nonvaccinates, 60% of the deltacya deltacrp vaccinates, and 15% of deltaphoP vaccinates were positive for S. hadar in tissues. In a second experiment, birds were vaccinated with either the deltaphoP strain or buffer and challenged with a 10-fold higher dose than in the first experiment. After challenge, all of the birds in both groups were colonized. The geometric mean number of cecal S. hadar isolated from the control group was 1.0 x 10(6) colony-forming units (CFU)/g, and from the vaccinated group, this value was 32 CFU/g, indicating a four to five log reduction in colonization by the challenge strain.
authors.affiliation: AVANT Immunotherapeutics, Inc., 8620 Pennell Drive, Overland, MO 63114, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Roland
authors.forename: Steven
authors.initials: S
authors.lastname: Tinge
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Warner
authors.forename: Donata
authors.initials: D
authors.lastname: Sizemore
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D018014:Gene Transfer Techniques; D007074:Immunoglobulin G / Q000276:immunology; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D014613:Vaccines, Attenuated
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated; C061064:PhoP protein, Bacteria