Complete cDNA sequence and mRNA expression of dog preproendothelin-3.
The full-length cDNA of dog preproendothelin-3 (PPET3) was cloned from lung tissue using RT-PCR and rapid amplification of cDNA ends. Aside from the poly (A) tail, the full-length cDNA was 1976 bp. A polyadenylation signal sequence and one copy of a consensus sequence, ATTTA, which is related to mRNA turnover, was found in the 3' noncoding region. The cDNA had a 594-bp open reading frame encoding a 198-amino acid polypeptide. Regions corresponding to a bioactive mature ET3 peptide, an intermediate form known as big-ET3, and an ET3-like peptide were observed in dog PPET3. Expression of PPET3 mRNA was detected throughout the organs examined, which included heart, lung, liver, kidney, spleen, stomach, pancreas, duodenum, colon, uterus, ovary and testis.
Fujimori
Yuki
Y
Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Uchide
Tsuyoshi
T
Temma
Kyosuke
K
Sasaki
Takushi
T
Kizaki
Keiichiro
K
Hara
Yukio
Y
Saida
Kaname
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D016232:Endothelins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018076:DNA, Complementary; D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; C075625:preproendothelin-3