title: The effect of sterol regulatory element-binding protein 2 polymorphism on the serum lipid in northern Chinese subjects.
abstract: Sterol regulatory element-binding protein 2 (SREBP-2) is an important nuclear transcription factor in the regulation of cellular cholesterol metabolism. To determine allele frequency of the 1784G>C polymorphism at the SREBP-2 locus and investigate the relationship between this polymorphism and serum lipid levels in Chinese people, we selected 486 individuals (118 men and 368 women) from the Xicheng District of Beijing. The subjects were divided into four groups: hypercholesterolemic subjects, hypertriglyceridemic subjects, combined hyperlipidemic subjects, and normal subjects. Serum lipid profiles were measured in all subjects, and 1784G>C was analyzed using polymerase chain reaction-restriction fragment length polymorphism analysis. There was no significant difference in genotype frequencies or allele frequencies of this polymorphism between the hyperlipidemic and control groups. The serum total cholesterol (TC) and LDL cholesterol (LDL-C) levels of the individuals carrying the C allele were higher than the noncarriers in both males and females in the hypercholesterolemic group, but statistical significance was only observed in females. The results of this study indicate that the SREBP-2 polymorphism is related to elevated concentrations of serum TC and LDL-C in hypercholesterolemic subjects. Further work is necessary to confirm the role of 1784G>C in the development of hyperlipidemia.
authors.affiliation: Department of Nutrition and Food Hygiene, Peking University Health Science Center, Beijing, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Duan
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Zhentao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jingjing
authors.initials: J
authors.lastname: Dao
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D015992:Body Mass Index; D002681:China; D008078:Cholesterol, LDL / Q000097:blood; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012737:Sex Factors; D051782:Sterol Regulatory Element Binding Protein 2; D014157:Transcription Factors / Q000235:genetics*; D014280:Triglycerides / Q000097:blood
chemical_list: D008078:Cholesterol, LDL; D004268:DNA-Binding Proteins; D008055:Lipids; C497390:SREBF2 protein, human; D051782:Sterol Regulatory Element Binding Protein 2; D014157:Transcription Factors; D014280:Triglycerides; D004247:DNA