title: Phenotypic and microarray gene expression analysis of tri-dimensional raft-modeled human head and neck squamous cell carcinoma.
abstract: OBJECTIVE
  To describe the phenotypic and gene expression differences in monolayer and tri-dimensional cultures systems.
  
  
  METHODS
  Normal oral epithelial cells (NOEC), primary head and neck squamous cell carcinoma (HNSCC), and HNSCC cell lines were used to create and study modeled tri-dimensional tissue. Using cDNA microarray analysis, monolayer and raft-modeled tri-dimensional HNSCC cell lines were compared.
  
  
  RESULTS
  NOEC, HNSCC, and both together can be modeled with tri-dimensional differentiation and cytokeratin characteristics analogous to in vivo tissue. Modeling of primary HNSCC resulted in a morphology of invasive HNSCC with areas of direct collagen invasion and MMP2 expression. Gene array analysis suggests that the individual cell lines themselves are the primary gene expression predictor and not the presence of tri-dimensional tissue architecture.
  
  
  CONCLUSIONS
  This tissue culture modeling system approximates the differentiation and tri-dimensional structure of in vivo tissues, and that raft modeled tri-dimensional HNSCC does not have a significantly different gene expression profile than the corresponding monolayer culture.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of North Carolina Hospitals, Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599, USA. Adam_Zanation@med.unc.edu
authors.forename: Adam M
authors.initials: AM
authors.lastname: Zanation
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yin
authors.forename: Carol
authors.initials: C
authors.lastname: Shores
authors.forename: Wendell G
authors.initials: WG
authors.lastname: Yarbrough
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003094:Collagen; D004847:Epithelial Cells; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D008954:Models, Biological; D009061:Mouth Mucosa; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D014407:Tumor Cells, Cultured
chemical_list: D003094:Collagen; D020778:Matrix Metalloproteinase 2