Platelet endothelial cell adhesion molecule deficiency or blockade significantly reduces leukocyte emigration in a majority of mouse strains.
PECAM is a molecule used specifically during the diapedesis step when neutrophils and monocytes leave the blood compartment. Anti-PECAM reagents, such as Abs and soluble fusion proteins, block diapedesis both in vivo and in vitro. However, the PECAM knockout mouse in C57BL/6 strain has no serious defects in most models of inflammation. We show in this study that the same PECAM knockout backcrossed into the FVB/n strain clearly has reduced leukocyte emigration in two models of inflammation. Furthermore, we show that anti-PECAM reagents can block leukocyte emigration in several other wild-type strains of mice like FVB/n, SJL, and the outbred strain Swiss Webster. This clearly shows that the C57BL/6 strain is uniquely able to compensate for the loss of PECAM function. Murine models of inflammatory disease that have been studied using C57BL/6 mice should be re-evaluated using FVB/n or other mouse strains to determine whether PECAM plays a role in those models.
Schenkel
Alan R
AR
Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. alan.schenkel@colostate.edu
Chew
Tina W
TW
Muller
William A
WA
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000235:genetics* / Q000276:immunology*; D003433:Crosses, Genetic; D003436:Croton Oil / Q000008:administration & dosage; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology*; D020868:Gene Silencing; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010538:Peritonitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D013045:Species Specificity; D013864:Thioglycolates / Q000008:administration & dosage
D019138:Antibodies, Blocking; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D013864:Thioglycolates; D003436:Croton Oil