title: Distribution patterns of matrix metalloproteinase (MMP)-2 and -9 and their inhibitors (TIMP-1 and TIMP-2) in the human decidua during early pregnancy.
abstract: Early human trophoblast shows dramatic invasive properties during early pregnancy. A tightly-regulated activation of matrix metalloproteinases (MMPs) is considered to be of critical importance for the control of trophoblast invasion. The aim of the present study was to determine MMP-2, MMP-9, TIMP-1 and TIMP-2 protein expression in decidual endometrium during the first trimester of pregnancy (22-42 days post coitus) with special attention to their expression patterns in endometrial compartments. Cytokeratin staining applied to adjacent sections was used to identify epithelial and trophoblast cells. We observed that MMP-2, particularly in the fourth week, appeared to be expressed more strongly in extravillous trophoblasts (EVTs) and vascular endothelial cells in the first trimester of pregnancy. Therefore, MMP-2 is likely to be the primary mediator in invasion of the trophoblast into the decidual endometrium, as well as in vascular remodeling and angiogenesis in the first trimester of pregnancy. The high expression of TIMP-1 and TIMP-2 in EVTs and glandular epithelium suggests that a restricted and balanced expression of these molecules is important for matrix remodeling and controlled trophoblast invasion during placentation. We conclude that (1) MMP-2 and MMP-9 and their inhibitors TIMP-1, and TIMP-2 determine the invasive behavior of trophoblast into the endometrium, and in particular, (2) MMP-2 may be the key regulator of trophoblast invasion in early human pregnancy.
authors.affiliation: Department of Histology and Embryology, University of Akdeniz, Faculty of Medicine, Antalya 07070, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Seval
authors.forename: Gökhan
authors.initials: G
authors.lastname: Akkoyunlu
authors.forename: Ramazan
authors.initials: R
authors.lastname: Demir
authors.forename: Mevlüt
authors.initials: M
authors.lastname: Asar
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D053552:Keratin-7; D007633:Keratins / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*; D014327:Trophoblasts / Q000502:physiology
chemical_list: D015415:Biomarkers; C507497:KRT7 protein, human; D053552:Keratin-7; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D007633:Keratins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9