HLA-Cw*1214 allele arisen via recombination between HLA-Cw*070201 and HLA-Cw*120201.
Allelic polymorphism of the major histocompatibility complex arises mostly from gene conversion. Intralocus gene conversion usually involves limited fragments of DNA, whereas recombination involving large fragments of DNA is considered to be a rare event. During routine sequencing-based typing of donors for the National Marrow Donor Program, a new HLA-C allele was identified in a Caucasian donor. The allele, HLA-Cw*1214, proved to be the product of recombination between HLA-Cw*070201 and HLA-Cw*120201. Exons 1, 2, the 3' end of exon 3 and exon 4 (with one mismatch) belong to HLA-Cw*120201, whereas part of exon 3 belongs to HLA-Cw*070201. Sequencing with primers based in exon 2 and exon 3 showed that intron 2 of the new allele also belonged completely to HLA-Cw*1202. The recombination event apparently occurred within exon 3 with the first point of recombination somewhere between codons 92 and 134 and the second one between codons 157 and 181.
Lebedeva
T V
TV
HLA Laboratory, American Red Cross Blood Services, New England Region, Dedham, MA 02026, USA. lebedevat@usa.redcross.org
Ohashi
M
M
Huang
A
A
Vasconcellos
S
S
Alosco
S M
SM
Kempenich
J
J
Yu
N
N
D000483:Alleles; D001483:Base Sequence; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014019:Tissue Donors; D044465:White People / Q000235:genetics
D015236:HLA-C Antigens