title: High-performance liquid chromatography multiresidue method for the determination of N-methyl carbamates in fruit and vegetable juices.
abstract: A rapid multiresidue method has been developed for the analysis of N-methylcarbamate insecticides (oxamyl, methomyl, propoxur, carbofuran, carbaryl, and methiocarb) in fruit and vegetable juices. The method is based on the adsorption of the N-methyl carbamates in Florisil and the subsequent extraction of pesticides using a low volume of acetone. Residue levels in juice were determined by reversed-phase high-performance liquid chromatography with fluorescence detection after postcolumn derivatization. The separation of carbamates is performed on a C8 column with water-methanol as mobile phase. Recovery studies were performed at 500-, 100-, and 10-ng/ml fortification levels, and average recoveries obtained for carbamates ranged from 79 to 109%, with relative standard deviations between 1.4 and 9.9%. The method was found to be linear over the range assayed from 10 to 1,000 ng/ml, and the detection limits for carbamates varied from 0.8 to 1.9 ng/ml.
authors.affiliation: Departamento de Medio Ambiente, INIA, Ctra. de la Coruña Km. 7.5, 28040 Madrid, Spain.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Sánchez-Brunete
authors.forename: Beatriz
authors.initials: B
authors.lastname: Albero
authors.forename: José Luis
authors.initials: JL
authors.lastname: Tadeo
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D004350:Drug Residues / Q000032:analysis*; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D007306:Insecticides / Q000032:analysis*; D012680:Sensitivity and Specificity; D014675:Vegetables / Q000737:chemistry
chemical_list: D007306:Insecticides