Wave2 activates serum response element via its VCA region and functions downstream of Rac.
WAVE2 is a member of the WASP/WAVE family of protein effectors of actin reorganization and cell movement. In this report, we demonstrate that WAVE2 overexpression induces serum response element (SRE) activation through serum response factor. A WAVE2 mutant lacking the VCA region did not induce SRE activation and actin polymerization. WAVE2-induced SRE activation was blocked by exposure of cells to Latrunculin A, or overexpression of actin mutant R62D. The DeltaVCA mutant inhibited Rac V12-induced SRE activation, suggesting that WAVE2 lies downstream of Rac. Similar deletion of the VCA domain of WASP attenuated Cdc42 V12-mediated SRE activation, suggesting that WAVE2 acts in relation to Rac as WASP acts in relation to Cdc42. WAVE2 overexpression did not activate NF-kappaB.
Ishiguro
Kazuhiro
K
Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
Cao
Zhifang
Z
Ilasca
Marco Lopez
ML
Ando
Takafumi
T
Xavier
Ramnik
R
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002460:Cell Line; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D027602:Serum Response Element; D015398:Signal Transduction; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014162:Transfection; D051300:Wiskott-Aldrich Syndrome Protein Family; D020744:rac GTP-Binding Proteins
D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D008840:Microfilament Proteins; D013844:Thiazoles; D053778:Thiazolidines; C492303:Wasf2 protein, mouse; D051300:Wiskott-Aldrich Syndrome Protein Family; D020744:rac GTP-Binding Proteins; C037067:latrunculin A