title: Rescue of Ebola virus from cDNA using heterologous support proteins.
abstract: Using the infectious clone for Zaire ebolavirus, the functional specificity of viral proteins of the ribonucleoprotein complex in transcription/replication was investigated by substituting them with heterologous proteins derived from closely (Reston ebolavirus) and distantly related filoviruses (Marburgvirus). The data clearly demonstrated that transcription/replication are neither strictly species-specific nor genus-specific. Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps. In contrast to previous data, viral proteins were able to target heterologous filovirus RNA. Together these results indicated that protein-protein interactions are more critical than protein-RNA interactions.
authors.affiliation: Special Pathogens Program, National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Health Canada, Canada.
authors.forename: Steven
authors.initials: S
authors.lastname: Theriault
authors.forename: Allison
authors.initials: A
authors.lastname: Groseth
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile; D002522:Chlorocebus aethiops; D018076:DNA, Complementary; D029043:Ebolavirus / Q000235:genetics* / Q000502:physiology; D016563:Filoviridae / Q000145:classification / Q000235:genetics* / Q000502:physiology; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
chemical_list: D015478:Antigens, Heterophile; D018076:DNA, Complementary; D019590:Nucleocapsid Proteins; D012261:Ribonucleoproteins; D014764:Viral Proteins