Production of L-asparaginase by filamentous fungi.
L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of glutamine and urea as nitrogen sources. These results suggest that L-asparaginase production by of filamentous fungi is under nitrogen regulation.
Sarquis
Maria Inez de Moura
MI
Laboratório de Coleção de Fungos, Departamento de Micologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. isarquis@ioc.fiocruz.br
Oliveira
Edna Maria Morais
EM
Santos
Alberdan Silva
AS
Costa
Gisela Lara da
GL
D001215:Asparaginase / Q000096:biosynthesis*; D001230:Aspergillus / Q000201:enzymology*; D003470:Culture Media; D005670:Fusarium / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology*
D003470:Culture Media; D001215:Asparaginase; D009584:Nitrogen