Effect of dietary vitamin E level on growth, tissue lipid peroxidation, and erythrocyte fragility of transgenic coho salmon, Oncorhynchus kisutch.
This study was conducted to investigate the effect of dietary vitamin E concentration on growth performance, iron-catalyzed lipid peroxidation in liver and muscle tissue, and erythrocyte fragility of transgenic growth hormone coho salmon (Oncorhynchus kisutch). Fish were fed one of four isoenergetic and isonitrogenous experimental diets that contained either 11, 29, 50, or 105 IU of vitamin E/kg. Following the 10-week feeding trial, no significant (P>0.05) diet-related differences were detected in growth, whole body proximate composition or erythrocyte fragility. The vitamin E contents of liver and muscle, however, were affected by the dietary treatment. Fish fed diets containing > or =50 IU of vitamin E/kg had significantly increased vitamin E concentrations in their tissues. Iron-catalyzed lipid peroxidation of liver and muscle tissue of fish fed elevated dietary vitamin E (> or =50 IU vitamin E/kg diet) was significantly lower (P<0.05) than that noted for fish fed the diet containing no supplemental vitamin E. The results indicated that changes in tissue lipid peroxidation measurements precede clinical signs of sub-optimal vitamin E intake.
Huang
Chen-Huei
CH
National Chiayi University, Department of Aquatic Biosciences, 300 University Road, Taiwan. chuhuang@mail.ncyu.edu.tw
Higgs
David A
DA
Balfry
Shannon K
SK
Devlin
Robert H
RH
D000821:Animal Feed; D000818:Animals; D030801:Animals, Genetically Modified; D001823:Body Composition; D001835:Body Weight; D004032:Diet; D004041:Dietary Fats; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D017684:Oncorhynchus kisutch; D013997:Time Factors; D024505:Tocopherols / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D004041:Dietary Fats; D014810:Vitamin E; D024505:Tocopherols