title: Isolation of a lactic acid bacterium and yeast consortium from a fermented material of Ulva spp. (Chlorophyta).
abstract: OBJECTIVE
  Microbiota in a fermented culture of Ulva spp. was examined with the objective to characterize the type of fermentation and to obtain starter microbes for performing seaweed fermentation.
  
  
  RESULTS
  Fermented Ulva spp. cultures which were obtained and transferred in a laboratory were examined for their microbiota. With phenotypic characterization and phylogenetic analysis based on rRNA gene nucleotide sequences, the predominant micro-organisms were identified as Lactobacillus brevis, Debaryomyces hanseni var. hansenii, and a Candida zeylanoides-related specimen, suggesting that the observed fermentation can be categorized to lactic acid and ethanol fermentation. Inoculating the individually cultured cell suspensions of the three kinds of micro-organisms with cellulase induced the fermentation in various kinds of seaweed.
  
  
  CONCLUSIONS
  A microbial consortium composed of a lactic acid bacterium, L. brevis, and yeasts, D. hansenii and a C. zeylanoides-related specimen, were predominant in a fermented culture of Ulva spp. Lactic acid and ethanol fermentation could be induced in various kinds of seaweed by adding this microbial consortium along with cellulase.
  
  
  CONCLUSIONS
  This is the first report of lactic acid and ethanol fermentation in seaweed, which is expected to provide a new material for food and dietary applications.
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Maruishi, Ohno, Saeki Hiroshima, Japan. uchida@affrc.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Murata
mesh_terms: D002175:Candida / Q000302:isolation & purification / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism; D021521:Ribotyping / Q000379:methods; D004718:Saccharomycetales / Q000302:isolation & purification / Q000378:metabolism; D012624:Seaweed / Q000378:metabolism; D044445:Ulva / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D019344:Lactic Acid; D000431:Ethanol