title: Cyclooxygenase-2 expression in deep endometriosis and matched eutopic endometrium.
abstract: OBJECTIVE
  To identify any relationship between cyclooxygenase-2 expression and the intensity of severe, endometriosis-related dysmenorrhea.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with deep endometriosis.
  
  
  METHODS
  During surgery, paired samples of tissue representing deep endometriosis and eutopic endometrium were obtained from 46 patients. Control endometrial tissue samples were obtained from 34 fertile women who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Pain assessment for dysmenorrhea was done with a 10-point linear analogue scale.
  
  
  METHODS
  The percentage of surface immunostained for Cox-2 was determined by an immunohistochemical technique. Relationships between pain score for dysmenorrhea and Cox-2 expression were analyzed.
  
  
  RESULTS
  Cox-2 expression was significantly higher in eutopic endometrial stromal cells from patients with deep endometriosis than in stroma from controls during the early, mid, and late secretory phases. In endometriosis patients, Cox-2 expression in eutopic endometrial stromal cells was significantly higher in women with more severe dysmenorrhea (pain score > or =7 vs. <7) during early and mid secretory phases.
  
  
  CONCLUSIONS
  Elevated Cox-2 expression in stromal cells in eutopic endometrium from patients with deep endometriosis may play a role in severe, endometriosis-related dysmenorrhea.
authors.affiliation: Department of Gynecology, Hôtel-Dieu, Polyclinique, CHU, Clermont-Ferrand, France. sachikoma@aol.com
authors.forename: Sachiko
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Michel
authors.initials: M
authors.lastname: Canis
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pouly
authors.forename: Arnaud
authors.initials: A
authors.lastname: Wattiez
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okamura
authors.forename: Gerard
authors.initials: G
authors.lastname: Mage
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D051546:Cyclooxygenase 2; D004412:Dysmenorrhea / Q000209:etiology / Q000503:physiopathology; D004715:Endometriosis / Q000150:complications / Q000201:enzymology* / Q000473:pathology; D004717:Endometrium / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism*; D008565:Membrane Proteins; D010147:Pain Measurement; D011446:Prospective Studies; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012720:Severity of Illness Index; D013194:Staining and Labeling; D017154:Stromal Cells / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases