title: Quantification of total furocoumarins in citrus oils by HPLC coupled with UV, fluorescence, and mass detection.
abstract: Furocoumarins or psoralens represent a class of photosensitizers whose use level is likely to be restricted to 1 ppm in cosmetic products by the EU. A reversed-phase HPLC method was developed to separate the 15 main furocoumarins present in citrus oils. Quantification by UV, fluorescence, or mass detectors was compared in terms of linearity and limit of detection. Cold-pressed oils of different citrus species were analyzed using this method. This method could be implemented in quality control laboratories equipped with an HPLC system and a UV diode array detector. Because of possible coelutions, the UV-spectral data should be carefully examined to avoid misleading interpretations of peaks.
authors.affiliation: Firmenich SA, Corporate R&D Division, Post Office Box 239, CH-1211 Geneva 8, Switzerland. eric.frerot@firmenich.com
authors.forename: Eric
authors.initials: E
authors.lastname: Frérot
authors.forename: Erik
authors.initials: E
authors.lastname: Decorzant
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D011564:Furocoumarins / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D011786:Quality Control
chemical_list: D011564:Furocoumarins; D010938:Plant Oils