title: Sequence analysis of the glycoproteins of Tomato chlorotic spot virus and Groundnut ringspot virus and comparison with other tospoviruses.
abstract: The tospoviruses Tomato chlorotic spot virus (TCSV) and Groundnut ringspot virus (GRSV) cause high economic losses in several vegetable crops in Brazil. The glycoprotein precursor coding sequence was still not available for these two viruses. In this study, the 3' 4 kb M RNA of TCSV and GRSV genome was cloned and sequenced. The sequences were compiled with the available 5' region sequence (NSM gene and 5' UTR) of the same isolates. The M RNA of TCSV was deduced as formed by 4,882 nucleotides, while of GRSV by 4,855 nucleotides. Both M RNA comprised two ORFs in an ambisense arrangement. The vcRNA ORF coded for viral glycoprotein (G1/G2) precursor of TCSV (128.46 kDa) and for glycoprotein precursor of GRSV (128.16 kDa). Comparison of the TCSV and GRSV glycoprotein precursor proteins with those of other tospoviruses showed the highest identity with Tomato spotted wilt virus (81 and 79%, respectively). The amino acid sequence comparison of glycoprotein precursor between TCSV and GRSV revealed a high identity of 92%. However, the nucleotide sequence of the M RNA intergenie region showed only 78%. Phylogenetic analysis was done based on glycoprotein precursor and on M RNA intergenic region of tospoviruses and parameters on tospovirus taxonomic classification were discussed.
authors.affiliation: Department of Cellular Biology, University of Brasília, DF, Brazil.
authors.forename: Fernanda Antinolfi
authors.initials: FA
authors.lastname: Lovato
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
authors.forename: Renato
authors.initials: R
authors.lastname: de Oliveira Resende
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: de Avila
authors.forename: Alice Kazuko
authors.initials: AK
authors.lastname: Inoue-Nagata
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001938:Brazil; D003001:Cloning, Molecular; D021901:DNA, Intergenic; D004279:DNA, Viral; D005814:Genes, Viral; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger; D012367:RNA, Viral; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D021901:DNA, Intergenic; D004279:DNA, Viral; D008562:Membrane Glycoproteins; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins