Interleukin-6, interleukin-8, and soluble tumor necrosis factor receptor-I in the cord blood as predictors of chronic lung disease in premature infants.
OBJECTIVE
In order to predict the late-development of chronic lung disease of prematurity (CLD), cytokines in the cord blood were assessed in this study.


METHODS
Eighteen premature infants with CLD were enrolled. Cord blood plasma levels of cytokines of these infants and 12 control infants without CLD were measured including interleukin (IL)-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, soluble TNF receptor-I, and soluble IL-6 receptor using a cytometric bead array and an enzyme-linked immunosorbent assay.


RESULTS
The cord blood IL-6, IL-8, and sTNFR-I levels were significantly elevated in CLD infants compared with those in control (P < .05). IL-1beta, IL-2, IL-4, IL-10, and IFN-gamma were undetectable in both groups. CLD infants with maternal chorioamnionitis had higher IL-6 than those without chorioamnionitis (P < .01). In CLD infants, IL-6 was higher in the infants who required prolonged oxygen therapy (P < .05).


CONCLUSIONS
Elevated inflammatory cytokines in the cord blood are associated with the progression to CLD.
An
Hiromi
H
Department of Pediatrics, Yokohama City University School of Medicine, Yokohama, Japan. hiromi.ann@nifty.com
Nishimaki
Shigeru
S
Ohyama
Makiko
M
Haruki
Atsushi
A
Naruto
Takuya
T
Kobayashi
Naoki
N
Sugai
Toshiyuki
T
Kobayashi
Yoshinori
Y
Mori
Masaaki
M
Seki
Kazuo
K
Yokota
Shumpei
S
D016022:Case-Control Studies; D002908:Chronic Disease; D016207:Cytokines / Q000097:blood*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008171:Lung Diseases / Q000097:blood / Q000175:diagnosis; D008297:Male; D011237:Predictive Value of Tests; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood; D012127:Respiratory Distress Syndrome, Newborn / Q000097:blood* / Q000175:diagnosis
D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D047888:Receptors, Tumor Necrosis Factor, Type I