Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid complex.
Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid [Cu(ssal)(2)(2+)] complex was studied. The cleavage was observed in a certain potential region where redox cycling of Cu(ssal)(2)(2+)/Cu(ssal)(2)(+) took place. Cu(ssal)(2)(2+) complex mediate generation of reactive oxygen species from O(2) by the Fenton reaction, these radicals are capable of damaging DNA. The cleaved DNA fragments were separated by high-performance liquid chromatography (HPLC). The experimental results indicated that the method for electrochemical cleavage of DNA by Cu(ssal)(2)(2+) complex was simple and efficient.
Wang
Yan-Ling
YL
School of Chemistry and Materials Science, Anhui Normal University, Wuhu 24100, PR China.
Liu
Yun-Chun
YC
Yang
Zhou-Sheng
ZS
Zhao
Guang-Chao
GC
D001557:Benzenesulfonates; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D017382:Reactive Oxygen Species / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*
D001557:Benzenesulfonates; D017382:Reactive Oxygen Species; D012459:Salicylates; D003300:Copper; D004247:DNA; C003366:sulfosalicylic acid