title: Gene amplifications in osteosarcoma-CGH microarray analysis.
abstract: Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high-resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes. Nineteen of the 22 cases had amplifications that on average spanned more than 1 Mb and contained more than 10 genes. Numerous regions of gain and loss were identified, and their boundaries were defined at high resolution. Novel amplicons were found at 14q11, 17q25, and 22q11-q13. Earlier-known large amplified regions were detected at 12q11-q15, 8q24, 6p12-p13, and 17p11-p13 in 8, 6, 5, and 4 of the 22 samples, respectively. Amplification of 12q was observed more frequently (36% of the cases) than previously reported. Previously known small amplicons at 1p34-p36, 1q21, 19q13, and 21q22 were seen in at least three cases. Our results implicate TOM1L2 and CYP27B1 as having roles as novel targets for the 17p and 12q amplicons, respectively. Details (www.helsinki.fi/cmg) of the amplified genes in each amplicon provide valuable raw data for further in silico studies.
authors.affiliation: Departments of Pathology and Medical Genetics, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Jassu
authors.initials: J
authors.lastname: Atiye
authors.forename: Maija
authors.initials: M
authors.lastname: Wolf
authors.forename: Sippy
authors.initials: S
authors.lastname: Kaur
authors.forename: Outi
authors.initials: O
authors.lastname: Monni
authors.forename: Tom
authors.initials: T
authors.lastname: Böhling
authors.forename: Aarne
authors.initials: A
authors.lastname: Kivioja
authors.forename: Eva
authors.initials: E
authors.lastname: Tas
authors.forename: Massimo
authors.initials: M
authors.lastname: Serra
authors.forename: Maija
authors.initials: M
authors.lastname: Tarkkanen
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012516:Osteosarcoma / Q000235:genetics*