title: Dominant B cell epitope from NY-ESO-1 recognized by sera from a wide spectrum of cancer patients: implications as a potential biomarker.
abstract: Monitoring the spontaneous antibody (Ab) response against a panel of relevant tumor-associated antigens (TAA) in cancer patients may provide useful information regarding the clinical status of cancer. However, current Ab detection approaches require the purification of recombinant proteins, which is often difficult to achieve. In order to bypass the purification of recombinant proteins, we identified a dominant B-cell epitope from a shared tumor antigen NY-ESO-1. A synthetic peptide of the epitope, ESO:1-40, was as sensitive as the recombinant protein for detecting Ab against NY-ESO-1 in most patients. NY-ESO-1 specific Ab present in the sera of patients with melanoma, prostate cancer, nonsmall cell lung cancer, esophageal cancer, gastric cancer and hepatocellular carcinoma reacted with the dominant peptide at a similar frequency as the recombinant protein. To our knowledge, ESO:1-40 is the first peptide epitope recognized by sera from a wide spectrum of cancer patients but not healthy donors. This simple and straightforward approach may allow the investigation of the clinical significance of spontaneous Ab responses against multiple TAA and their correlation with the clinical course of malignant diseases in the future.
authors.affiliation: Department of Urology and Jonsson Comprehensive Cancer Center, 66-118 Center for Health Sciences, David Geffen School of Medicine at UCLA, Box 951738, Los Angeles, CA 90095, USA. gzeng@mednet.ucla.edu
authors.forename: Gang
authors.initials: G
authors.lastname: Zeng
authors.forename: Michael E
authors.initials: ME
authors.lastname: Aldridge
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Allan J
authors.initials: AJ
authors.lastname: Pantuck
authors.forename: Allen Y
authors.initials: AY
authors.lastname: Wang
authors.forename: Yue-xiang
authors.initials: YX
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Yuan
authors.forename: Paul F
authors.initials: PF
authors.lastname: Robbins
authors.forename: Steven M
authors.initials: SM
authors.lastname: Dubinett
authors.forename: Jean B
authors.initials: JB
authors.lastname: deKernion
authors.forename: Arie S
authors.initials: AS
authors.lastname: Belldegrun
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology; D008545:Melanoma / Q000235:genetics / Q000276:immunology*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; D014408:Biomarkers, Tumor; C104948:CTAG1B protein, human; D017931:DNA Primers; D000939:Epitopes; D008565:Membrane Proteins; D010446:Peptide Fragments