Genotyping accuracy for whole-genome amplification of DNA from buccal epithelial cells.
We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/phi29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.
Leviel
Keren
K
Department of Genetics, University of North Carolina at Chapel Hill, 27599-7264, USA.
Olarte
Megan
M
Sullivan
Patrick F
PF
D004247:DNA / Q000032:analysis*; D004847:Epithelial Cells; D005784:Gene Amplification / Q000235:genetics; D016678:Genome; D005838:Genotype; D006801:Humans; D008214:Lymphocytes; D009061:Mouth Mucosa
D004247:DNA