Detection of insulin-antibody binding on a solid surface using imaging ellipsometry.
Imaging ellipsometry (IE) was used to detect the binding of insulin to its antibody on a solid surface. The modification of a gold surface with 11-mecaptoundecanoic acid (11-MUA), the adsorption of protein G, and antibody immobilization onto the protein G layer were confirmed by surface plasmon resonance. Ellipsometric images and ellipsometric angles of the surface antibody were acquired using the IE system by off-null ellipsometry. Ellipsometric images of antigen binding to the antibody were acquired, and their mean optical intensities estimated. Changes in mean optical intensity indicated that the detection range for insulin was from 10 ng/ml to 100 microg/ml.
Bae
Young Min
YM
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul 121-742, South Korea.
Oh
Byung-Keun
BK
Lee
Woochang
W
Lee
Won Hong
WH
Choi
Jeong-Woo
JW
D000936:Antigen-Antibody Complex / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007328:Insulin / Q000032:analysis* / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry*; D012031:Refractometry / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D000936:Antigen-Antibody Complex; D020099:Coated Materials, Biocompatible; C029152:G-substrate; D007328:Insulin; D009419:Nerve Tissue Proteins