Genistein induces Gadd45 gene and G2/M cell cycle arrest in the DU145 human prostate cancer cell line.
Genistein is the most abundant isoflavone of soybeans and has been shown to cause growth arrest in various human cancer cell lines. However, the precise mechanism for this is still unclear. We report here that the growth arrest and DNA damage-inducible gene 45 (gadd45) gene is induced by genistein via its promoter in a DU145 human prostate cancer cell line. The binding of transcription factor nuclear factor-Y to the CCAAT site of the gadd45 promoter appears to be important for this activation by genistein.
Oki
Teruki
T
Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Sowa
Yoshihiro
Y
Hirose
Tohru
T
Takagaki
Nobumasa
N
Horinaka
Mano
M
Nakanishi
Ryoko
R
Yasuda
Chikako
C
Yoshida
Tatsushi
T
Kanazawa
Motohiro
M
Satomi
Yoshiko
Y
Nishino
Hoyoku
H
Miki
Tsuneharu
T
Sakai
Toshiyuki
T
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D017931:DNA Primers; D024202:Electrophoretic Mobility Shift Assay; D016195:G2 Phase / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*
D017931:DNA Primers; D019833:Genistein