Effects of cured dentin bonding materials on human monocyte viability.
OBJECTIVE
Dentin Bonding Agents (DBAs) have been proposed as root-end filling materials. This study examined the effect of polymerized DBAs on human monocyte viability.


METHODS
Monocytes were directly isolated from peripheral blood and being exposed to cured Scotch bond I (Single Bond) and Prime & Bond in different time intervals (36 and 72 hours). The viability of monocytes was determined by MTT assay.


RESULTS
Viability of the cells was time dependent. There was no significant difference between the effect of 2 DBAs on monocytes.


CONCLUSIONS
Results indicate that DBAs in polymerized form can alter the viability of monocytes and decrease it within time.
Vahid
A
A
Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. vahidala@sina.tums.ac.ir
Hadjati
J
J
Kermanshah
H
H
Ghabraei
S
S
D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003188:Composite Resins / Q000494:pharmacology; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D009000:Monocytes / Q000187:drug effects*; D011109:Polymethacrylic Acids / Q000494:pharmacology; D019279:Resin Cements / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D011109:Polymethacrylic Acids; C103500:Prime & Bond; D019279:Resin Cements; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C041330:Scotchbond; C022616:thiazolyl blue