title: [Molecular analysis of methicillin-resistant staphylococci isolated from hospital patients and in the out-patient clinic].
abstract: In this study, a molecular analysis of the methicillin-resistant coagulase-negative staphylococci strains was performed. The obtained results of the biochemical and drug resistant pattern investigations were insufficient to assess the relationship between the strains. Therefore genotyping by the restriction fragment length polymorphism analysis (PCR-RFLP) method was performed. Analyzed strains characterized presence of the mecA gene-PCR products. The PCR products were digested with DraI and TasI, and the fragments separated by agarose gel electrophoresis. Typing of the methicillin-resistant gene using PCR-RFLP showed that all MRCNS strains possess an identical restriction pattern of the mecA gene. This identical restriction pattern of the mecA gene in investigated strains may suggest an easy transfer of this gene between different staphylococci species and lead to the spreading of methicillin-resistant among hospital strains. Furthermore performing the comparison of different phenotype and genotype methods has shown that the PCR-RFLP method is quick and reliable, enabling the detection and estimation of the relationship between MRCNS strains.
authors.affiliation: Zakład Ogólnej Biologii Lekarskiej Slaskiej, Akademii Medycznej w Katowicach.
authors.forename: Oliwia
authors.initials: O
authors.lastname: Małecka-Mikosz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wiczkowski
authors.forename: Joanna
authors.initials: J
authors.lastname: Strzelczyk
authors.forename: Marzena
authors.initials: M
authors.lastname: Zalewska-Ziob
authors.forename: Lucja
authors.initials: L
authors.lastname: Dyla
authors.forename: Mirosław
authors.initials: M
authors.lastname: Kwaśniewski
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D006801:Humans; D007297:Inpatients; D016106:Methicillin Resistance / Q000235:genetics*; D010045:Outpatients; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; C487495:MecA protein, Staphylococcus epidermidis; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus