title: Insights into assembly from structural analysis of bacteriophage PRD1.
abstract: The structure of the membrane-containing bacteriophage PRD1 has been determined by X-ray crystallography at about 4 A resolution. Here we describe the structure and location of proteins P3, P16, P30 and P31. Different structural proteins seem to have specialist roles in controlling virus assembly. The linearly extended P30 appears to nucleate the formation of the icosahedral facets (composed of trimers of the major capsid protein, P3) and acts as a molecular tape-measure, defining the size of the virus and cementing the facets together. Pentamers of P31 form the vertex base, interlocking with subunits of P3 and interacting with the membrane protein P16. The architectural similarities with adenovirus and one of the largest known virus particles PBCV-1 support the notion that the mechanism of assembly of PRD1 is scaleable and applies across the major viral lineage formed by these viruses.
authors.affiliation: Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK.
authors.forename: Nicola G A
authors.initials: NG
authors.lastname: Abrescia
authors.forename: Joseph J B
authors.initials: JJ
authors.lastname: Cockburn
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Grimes
authors.forename: Geoffrey C
authors.initials: GC
authors.lastname: Sutton
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Diprose
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Butcher
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Fuller
authors.forename: Carmen
authors.initials: C
authors.lastname: San Martín
authors.forename: Roger M
authors.initials: RM
authors.lastname: Burnett
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: Dennis H
authors.initials: DH
authors.lastname: Bamford
authors.forename: Jaana K H
authors.initials: JK
authors.lastname: Bamford
mesh_terms: D000595:Amino Acid Sequence; D025622:Bacteriophage PRD1 / Q000737:chemistry* / Q000648:ultrastructure*; D002213:Capsid / Q000737:chemistry / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry* / Q000648:ultrastructure; D019065:Virus Assembly
chemical_list: D021122:Protein Subunits; D015678:Viral Structural Proteins