title: Methyl jasmonate induces apoptosis through induction of Bax/Bcl-XS and activation of caspase-3 via ROS production in A549 cells.
abstract: Jasmonates are plant lipid derivatives, similar to mammalian eicosanoid, that play a critical role(s) in plant defenses against herbivores and pathogens through up-regulating the expression of defense-related genes. Recently, jasmonates were shown to induce cell cycle arrest or apoptosis in human leukemia, prostate and breast cancer cells, but not in normal lymphocytes, suggesting that the chemicals can be used as a novel class of anti-cancer drugs. In the present study, we examined the molecular mechanism that contributes to methyl jasmonate-induced apoptosis. Herein we show that methyl jasmonate induces apoptosis through induction of Bax/Bcl-XS and activation of caspase-3 via reactive oxygen species production in A549 human lung adenocarcinoma cells.
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kim
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Lee
authors.forename: Su Young
authors.initials: SY
authors.lastname: Oh
authors.forename: Song Iy
authors.initials: SI
authors.lastname: Han
authors.forename: Hye Gyeong
authors.initials: HG
authors.lastname: Park
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Yoo
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Kang
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D003517:Cyclopentanes / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
chemical_list: D000085:Acetates; C494826:BAX protein, human; C494811:BCL2L1 protein, human; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C072239:methyl jasmonate; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases