A natural vaccinia virus promoter with exceptional capacity to direct protein synthesis.
A survey of vaccinia virus promoters, through a reporter gene approach, has identified the viral I1L promoter as having exceptional activity. The I1L promoter exhibited over 10 times the activity of other vaccinia promoters and even rivaled the activity of the bacteriophage T7 promoter in the hybrid vaccinia/T7 expression system. The I1L promoter had high activity in both transient transfection experiments and in the context of recombinant viruses. The I1L promoter should be useful for high-level protein synthesis and poxvirus studies in general.
Liu
Xu
X
Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, IN 47907-1153, USA.
Kremer
Marcia
M
Broyles
Steven S
SS
D017123:Bacteriophage T7 / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014176:Protein Biosynthesis; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*