Vibrio crassostreae sp. nov., isolated from the haemolymph of oysters (Crassostrea gigas).
Polyphasic analysis of five new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T)=LMG 22240(T)=CIP 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO(2) production and the presence of the fatty acids 14 : 0 iso and 16 : 0 iso.
Faury
N
N
Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
Saulnier
D
D
Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
Thompson
F L
FL
Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
Gay
M
M
Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
Swings
J
J
Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
Roux
F Le
FL
Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D006458:Hemolymph / Q000382:microbiology*; D006867:Hydrolases / Q000032:analysis; D008969:Molecular Sequence Data; D009585:Nitrogen Dioxide / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010125:Ostreidae / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification*; D001616:beta-Galactosidase / Q000032:analysis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006867:Hydrolases; D001616:beta-Galactosidase; C019723:arginine deiminase; D027081:DNA Gyrase; D009585:Nitrogen Dioxide