Arabidopsis WPP-domain proteins are developmentally associated with the nuclear envelope and promote cell division.
The nuclear envelope (NE) acts as a selective barrier to macromolecule trafficking between the nucleus and the cytoplasm and undergoes a complex reorganization during mitosis. Different eukaryotic kingdoms show specializations in NE function and composition. In contrast with vertebrates, the protein composition of the NE and the function of NE proteins are barely understood in plants. MFP1 attachment factor 1 (MAF1) is a plant-specific NE-associated protein first identified in tomato (Lycopersicon esculentum). Here, we demonstrate that two Arabidopsis thaliana MAF1 homologs, WPP1 and WPP2, are associated with the NE specifically in undifferentiated cells of the root tip. Reentry into cell cycle after callus induction from differentiated root segments reprograms their NE association. Based on green fluorescent protein fusions and immunogold labeling data, the proteins are associated with the outer NE and the nuclear pores in interphase cells and with the immature cell plate during cytokinesis. RNA interference-based suppression of the Arabidopsis WPP family causes shorter primary roots, a reduced number of lateral roots, and reduced mitotic activity of the root meristem. Together, these data demonstrate the existence of regulated NE targeting in plants and identify a class of plant-specific NE proteins involved in mitotic activity.
Patel
Shalaka
S
Plant Biotechnology Center and Department of Plant Molecular and Cellular Biology, The Ohio State University, Columbus, Ohio 43210, USA.
Rose
Annkatrin
A
Meulia
Tea
T
Dixit
Ram
R
Cyr
Richard J
RJ
Meier
Iris
I
D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D018519:Meristem / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D017434:Protein Structure, Tertiary / Q000235:genetics; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid
C466201:AT3G63130 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D020690:GTPase-Activating Proteins; D036961:Matrix Attachment Region Binding Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; C501409:WPP1 protein, Arabidopsis; C501410:WPP2 protein, Arabidopsis