Potentiometric immunosensor using artificial antibody based on molecularly imprinted polymers.
A potentiometric artificial immunosensor based on a molecularly imprinted polymer was prepared as a detecting element in micro total analysis systems with the intent of providing easy clinical analysis. As the structure and transducing mechanism of this sensor are very simple, construction of a single microsensor should be quite easy. Multimicrosensor arrays applicable to several kinds of analytes will be attainable by both changing the template molecule to be imprinted and reducing the sensor size. The response characteristics of this sensor were evaluated by measuring the response potential to serotonin, which was used as a model material. The obtained sensor was highly responsive to serotonin in water but not to tryptamine, acetaminophen, or procainamide. This phenomenon confirms that the sensor recognizes serotonin and that it functions as a specific artificial immunosensor. Quick measurement is possible because the response time, defined as the time required to achieve 95% of the magnitude of the equilibrated signal, correspond to approximately 12 s. The sensor's determination and detection limits were found to be 1 mumol/L and 100 pmol/L, respectively. These results suggest that our strategy can be applied to construction of a potentiometric artificial immunosensor.
Kitade
Tatsuya
T
Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan. kitade@mb.kyoto-phu.ac.jp
Kitamura
Keisuke
K
Konishi
Tomoyuki
T
Takegami
Shigehiko
S
Okuno
Takayoshi
T
Ishikawa
Michie
M
Wakabayashi
Manabu
M
Nishikawa
Kayoko
K
Muramatsu
Yoko
Y
D000082:Acetaminophen / Q000032:analysis; D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D002138:Calibration; D011108:Polymers / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation*; D011342:Procainamide / Q000032:analysis; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis; D014363:Tryptamines / Q000032:analysis
D000906:Antibodies; D011108:Polymers; D014363:Tryptamines; D012701:Serotonin; D000082:Acetaminophen; C030820:tryptamine; D011342:Procainamide