A novel immunosensor based on immobilization of hepatitis B surface antibody on platinum electrode modified colloidal gold and polyvinyl butyral as matrices via electrochemical impedance spectroscopy.
Hepatitis B surface antibody (HBsAb) was immobilized to the surface of platinum electrode modified with colloidal gold and polyvinyl butyral (PVB) as matrices to detect hepatitis B surface antigen (HBsAg) via electrochemical impedance spectroscopy (EIS). The electrochemical measurements of cyclic voltammetry and impedance spectroscopy showed that K(4)[Fe(CN)(6)]/K(3)[Fe(CN)(6)] reactions on the platinum electrode surface were blocked due to the procedures of self-assembly of HBsAb-Au-PVB. The binding of a specific HBsAb to HBsAg recognition layer could be detected by measurements of the impedance change. A new strategy was introduced for improving the sensitivity of impedance measurements via the large specific surface area and high surface free energy of Au nanoparticles and the encapsulated effect of polyvinyl butyral. The results showed that this strategy caused dramatic improvement of the detection sensitivity of HBsAg and had good linear response to detect HBsAg in the range of 20-160 ng.ml(-1) with a detection limit of 7.8 ng.ml(-1). Moreover, the studied immunosensor exhibited high sensitivity and long-term stability.
Tang
Dianping
D
Chong Qing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, People's Republic of China.
Yuan
Ruo
R
Chai
Yaqin
Y
Dai
Jianyuan
J
Zhong
Xia
X
Liu
Yan
Y
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D017979:Gold Colloid / Q000737:chemistry*; D006510:Hepatitis B Antibodies / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D007103:Immobilization; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010984:Platinum / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D013057:Spectrum Analysis
D017979:Gold Colloid; D006510:Hepatitis B Antibodies; D011145:Polyvinyls; D010984:Platinum; C027464:polyvinylbutyral