title: Type II isopentenyl diphosphate isomerase from Synechocystis sp. strain PCC 6803.
abstract: Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni(2+) chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg(2+) for activity; K(m)(IPP) was 52 microM, and k(cat)(IPP) was 0.23 s(-1).
authors.affiliation: Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Sam J
authors.initials: SJ
authors.lastname: Barkley
authors.forename: Shrivallabh B
authors.initials: SB
authors.lastname: Desai
authors.forename: C Dale
authors.initials: CD
authors.lastname: Poulter
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000302:isolation & purification* / Q000378:metabolism; D045782:Hemiterpenes; D007700:Kinetics; D046939:Synechocystis / Q000201:enzymology*
chemical_list: D045782:Hemiterpenes; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase