Kaistella koreensis gen. nov., sp. nov., a novel member of the Chryseobacterium-Bergeyella-Riemerella branch.
Gram-negative, non-spore-forming, rod-shaped, yellow-pigmented bacteria isolated from a freshwater stream in Korea were investigated to determine their taxonomic position. Complete 16S rRNA gene sequence analysis indicated that the organisms should be placed in the Chryseobacterium-Bergeyella-Riemerella branch in the family Flavobacteriaceae. Phylogenetically, the strains were most closely related to Chryseobacterium balustinum ATCC 33487(T) and Chryseobacterium scophthalmum LMG 13028(T) (94.3 and 94.1 % 16S rRNA gene sequence similarity, respectively) and they clustered on a separate well-supported branch. The strains contained menaquinone MK-6 as the predominant respiratory quinone and showed higher G+C contents (41.7 mol%) than other species in the Chryseobacterium-Bergeyella-Riemerella branch and i-C(15 : 0) as a major fatty acid (47-52 %). The phylogenetic distances from any species with validly published names and their phenotypic properties confirmed that the strains constitute a separate species in a new genus, for which the name Kaistella koreensis gen. nov., sp. nov. is proposed (type strain Chj707(T)=KCTC 12107(T)=IAM 15050(T)).
Kim
Myung Kyum
MK
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Im
Wan-Taek
WT
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Shin
Yong Kook
YK
Division of Genome Resources Bank and Reservation, National Genome Research Institute, Korean National Institute of Health, Seoul 122-701, Republic of Korea.
Lim
Ju Hyoung
JH
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Kim
Sung-Hye
SH
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Lee
Byung Chul
BC
Laboratory of Rickettsial and Zoonotic Disease, Department of Microbiology, Korean National Institute of Health, Seoul 122-701, Republic of Korea.
Park
Mi-Yeoun
MY
Laboratory of Rickettsial and Zoonotic Disease, Department of Microbiology, Korean National Institute of Health, Seoul 122-701, Republic of Korea.
Lee
Ki Young
KY
Department of Food and Biotechnology, Hoseo University, Asan 336-795, Republic of Korea.
Lee
Sung-Taik
ST
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kuseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D045247:Chryseobacterium / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D041965:Flavobacteriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D005840:Gentian Violet; D007723:Korea; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological; D011809:Quinones / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet