EAG and behavioral responses of Helicoverpa armigera males to volatiles from poplar leaves and their combinations with sex pheromone.
Electroantennogram (EAG) evaluation of selected compounds from wilted leaves of black poplar, Populus nigra, showed that phenyl acetaldehyde, methyl salicylate, (E)-2-hexenal elicited strong responses from male antennae of Helicoverpa armigera. When mixed with sex pheromone (Ph), some volatiles, e.g. phenyl acetaldehyde, benzyl alcohol, phenylethanol, methylsalicylate, linalool, benzaldehyde, (Z)-3-hexenol, (Z)-3-hexenylacetate, (Z)-6-nonenol, cineole, (E)-2-hexenal, and geraniol elicited stronger responses from male antennae than Ph alone. Wind tunnel bioassay demonstrated that various volatiles could either enhance or inhibit the effect of synthetic sex pheromone. (E)-2-hexenal, (Z)-3-hexenol and linalool in combination with Ph could not induce any male to land on source at all, whereas phenyl acetaldehyde, benzaldehyde, (Z)-6-nonenol and salicylaldehyde combined with Ph enhanced male response rates by 58.63%, 50.33%, 51.85% and 127.78%, respectively, compared to Ph alone. These results suggested that some volatiles should modify sex pheromone caused behavior and that some of them could possibly be used as a tool for disrupting mating or for enhancing the effect of synthetic sex pheromone in the field.
Deng
Jian-Yu
JY
Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. jydeng70@hotmail.com
Huang
Yong-Ping
YP
Wei
Hong-Yi
HY
Du
Jia-Wei
JW
D000818:Animals; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005426:Flight, Animal / Q000502:physiology; D007915:Lepidoptera / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D009812:Odorants; D009930:Organic Chemicals / Q000494:pharmacology*; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D032107:Populus / Q000378:metabolism*; D012724:Sex Attractants / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D014835:Volatilization
D004338:Drug Combinations; D009930:Organic Chemicals; D010936:Plant Extracts; D012724:Sex Attractants