Fine mapping of chromosome 10q deletions in mycosis fungoides and sezary syndrome: identification of two discrete regions of deletion at 10q23.33-24.1 and 10q24.33-25.1.
Previous cytogenetic studies in mycosis fungoides (MF) and Sezary syndrome (SS) have identified a large and poorly defined area of chromosomal deletion on chromosome 10q. We report an extensive fine-mapping allelotyping study using 19 microsatellite markers in the region 10q22.3-10q26.13. Allelic loss was identified by loss of heterozygosity analysis in 26 of 60 (43%) cases: 15 of 45 (33%) with MF and 11 of 15 (73%) with SS. MF and SS samples showed similar patterns of allelic loss with the identification of two discrete regions of deletion which were mutually exclusive in all but two cases. Within the first region of deletion at 10q23.33-10q24.1, around microsatellite marker D10S185 (2.77 Mb), 23 genes were identified, including three (KIF11, HHEX, and HELLS) with functions that, if dysregulated, could be critical in MF and SS. The second region of deletion, 10q24.33-10q25.1, around microsatellite marker D10S530 (3.92 Mb), encodes 11 genes, the majority of which have poorly identified functions. This extensive allelotyping study provides the basis for future highly selective candidate gene analyses.
Wain
E Mary
EM
Skin Tumour Unit, St. John's Institute of Dermatology, St. Thomas' Hospital, London, UK. marywain@doctors.net.uk
Mitchell
Tracey J
TJ
Russell-Jones
Robin
R
Whittaker
Sean J
SJ
D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D009182:Mycosis Fungoides / Q000235:genetics*; D012751:Sezary Syndrome / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics*