title: High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging.
abstract: We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.
authors.affiliation: Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Requejo-Isidro
authors.forename: J
authors.initials: J
authors.lastname: McGinty
authors.forename: I
authors.initials: I
authors.lastname: Munro
authors.forename: D S
authors.initials: DS
authors.lastname: Elson
authors.forename: N P
authors.initials: NP
authors.lastname: Galletly
authors.forename: M J
authors.initials: MJ
authors.lastname: Lever
authors.forename: M A A
authors.initials: MA
authors.lastname: Neil
authors.forename: G W H
authors.initials: GW
authors.lastname: Stamp
authors.forename: P M W
authors.initials: PM
authors.lastname: French
authors.forename: P A
authors.initials: PA
authors.lastname: Kellett
authors.forename: J D
authors.initials: JD
authors.lastname: Hares
authors.forename: A K L
authors.initials: AK
authors.lastname: Dymoke-Bradshaw
mesh_terms: D000465:Algorithms; D019723:Endoscopes; D004724:Endoscopy / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods