Paenibacillus elgii sp. nov., with broad antimicrobial activity.
Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol%, and the major fatty acid was anteiso-C(15 : 0) (54.1 %). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 % with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 %, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)).
Kim
Dal-Soo
DS
LG Life Sciences Ltd/R&D Park, #104-1 Moonji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
Bae
Cheol-Yong
CY
LG Life Sciences Ltd/R&D Park, #104-1 Moonji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
Jeon
Jae-Jin
JJ
LG Life Sciences Ltd/R&D Park, #104-1 Moonji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
Chun
Sam-Jae
SJ
LG Life Sciences Ltd/R&D Park, #104-1 Moonji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
Oh
Hyun Woo
HW
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Hong
Soon Gyu
SG
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Baek
Keun-Sik
KS
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Moon
Eun Young
EY
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Bae
Kyung Sook
KS
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D031344:Perilla frutescens / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S