[Predicting cancerization of condyloma acuminatum by testing expression of p16].
OBJECTIVE
About 3%-10% of condyloma acuminatum (CA) may develop into cancer. Some studies indicated that homologous deletion of p16 gene is a major factor that causes cancerization of CA. This study was to detect expression of P16 protein in CA tissues and its cancerization tissues, and to investigate relationship of abnormal expression of P16 and cancerization of CA.


METHODS
A total of 75 skin biopsy specimens were collected, including 30 normal skin samples (control group), 35 CA samples, and 10 cancerized CA samples. Expression of P16 was tested by LSAB immunohistochemistry, and relationship of P16 and cancerization of CA was statistically analyzed.


RESULTS
CA and normal skin tissues showed weakly positive expression of P16, no significant difference exist (P< 0.05). Cancerized CA tissues showed positive or strongly positive expression of P16, significantly stronger than CA and normal skin tissues (P< 0.05).


CONCLUSIONS
Positive and strongly positive expression of P16 in CA tissue implied risk of cancerization of CA. P16 may be a useful predictor for cancerization of CA.
Su
Xiang-Yang
XY
Department of Dermatology and Venerology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510 630, P.R. China. sh3355@vip.163.com
Lai
Wei
W
Zhu
Hui-Lan
HL
Su
Zu-Lan
ZL
Guo
Shao-Zhong
SZ
Lin
Su-Xia
SX
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D003218:Condylomata Acuminata / Q000378:metabolism* / Q000473:pathology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010409:Penile Diseases / Q000378:metabolism* / Q000473:pathology; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000378:metabolism; D014623:Vaginal Diseases / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16