Trypanocidal activity of a new diterpene from Casearia sylvestris var. lingua.
Bioassay-guided fractionation of the hexanic root bark extract of Casearia sylvestris var. lingua led to the isolation of a new clerodane diterpene, whose structure was elucidated as rel-(2 S,5 R,6 R,8 S,9 S,10 R,18 S,19 R)-19-acetoxy-18,19-epoxy-6-hydroxy-18-butanoyloxy-2-(2-methylbutanoyloxy)cleroda-3,13(16), 14-triene by spectroscopic means, including 1D and 2D NMR analyses. This compound showed pronounced activity on Trypanosoma cruzi, the casual agent of Chagas' disease, with minimal inhibitory concentration (MIC) at 0.59 microg/mL.
Espindola
Laila Salmen
LS
Laboratório de Farmacognosia, Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, Brazil. darvenne@unb.br
Vasconcelos Júnior
José Rossy e
JR
de Mesquita
Mariana Laundry
ML
Marquié
Patrick
P
de Paula
José Elias
JE
Mambu
Lengo
L
Santana
Jaime Martins
JM
D000818:Animals; D037821:Casearia; D014355:Chagas Disease / Q000188:drug therapy; D006801:Humans; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000187:drug effects*
D010936:Plant Extracts; D014344:Trypanocidal Agents