title: Cloning and expression of the yolk protein of the tsetse fly Glossina morsitans morsitans.
abstract: Two major families of nutritional proteins exist in insects, namely the vitellogenins and the yolk proteins. While in other insects only vitellogenins are found, cyclorraphan flies only contain yolk proteins. Possible sites of yolk protein synthesis are the fat body and the follicle cells surrounding the oocyte. We report the cloning of the yolk protein of the tsetse fly Glossina morsitans morsitans, a species with adenotrophic viviparity. The tsetse fly yolk protein could be aligned with other dipteran yolk proteins and with some vertebrate lipases. In contrast to the situation in most fly species, only a single yolk protein gene was found in the tsetse fly. Northern blot analysis showed that only the ovarian follicle cells, and not the fat body represents the site of yolk protein synthesis.
authors.affiliation: Department of Biology, Catholic University of Leuven, Naamsestraat 59, 3000 Leuven, Belgium. korneel.hens@bio.kuleuven.ac.be
authors.forename: Korneel
authors.initials: K
authors.lastname: Hens
authors.forename: Nathalie
authors.initials: N
authors.lastname: Macours
authors.forename: Ilse
authors.initials: I
authors.lastname: Claeys
authors.forename: Carmen
authors.initials: C
authors.lastname: Francis
authors.forename: Roger
authors.initials: R
authors.lastname: Huybrechts
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005216:Fat Body / Q000737:chemistry; D005260:Female; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000737:chemistry; D012098:Reproduction; D017386:Sequence Homology, Amino Acid; D014370:Tsetse Flies / Q000737:chemistry* / Q000235:genetics*
chemical_list: D004527:Egg Proteins