title: [Assessment of the diagnostic value of RT-PCR on amniotic fluid for prenatal diagnosis of congenital rubella infection].
abstract: OBJECTIVE
  To assess the diagnostic value of RT-PCR on amniotic fluid (AF) for prenatal diagnosis of congenital rubella infection.
  
  
  METHODS
  RT-PCR on AF was compared to specific IgM antibody detection in foetuses and/or newborns in 45 pregnant women with confirmed primary infection.
  
  
  RESULTS
  specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%.
  
  
  CONCLUSIONS
  RT PCR may be considered as a valuable tool for prenatal diagnosis of foetal rubella infection.
authors.affiliation: Service de microbiologie-immunologie biologique, hôpital Antoine-Béclère, 92141 Clamart, France.
authors.forename: M
authors.initials: M
authors.lastname: Macé
authors.forename: D
authors.initials: D
authors.lastname: Cointe
authors.forename: C
authors.initials: C
authors.lastname: Six
authors.forename: D
authors.initials: D
authors.lastname: Levy-Bruhl
authors.forename: I
authors.initials: I
authors.lastname: Parent du Chatelet
authors.forename: D
authors.initials: D
authors.lastname: Ingrand
authors.forename: L
authors.initials: L
authors.lastname: Grangeot-Keros
mesh_terms: D000653:Amniotic Fluid / Q000821:virology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011296:Prenatal Diagnosis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000235:genetics* / Q000302:isolation & purification