title: Determination of thiol compounds in rat striatum microdialysate by HPLC with a nanosized CoHCF-modified electrode.
abstract: A cobalt hexacyanoferrate (CoHCF) nanoparticle (size ca. 60 nm) chemically modified electrode (CME) was fabricated and the electrochemical behavior of thiols at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon (GC) electrode and with a general CoHCF CME which was electrode-posited in the traditional manner, the present nanosized CoHCF CME efficiently performed electrocatalytic oxidation for glutathione (GSH) and L-Cysteine (L-Cys) with relatively high sensitivity, outstanding stability, and long-life. Combined with high-performance liquid chromatography (HPLC), the nanosized CoHCF CME was used for electrochemical determination (ECD) of GSH and L-Cys. The peak currents were a linear function of concentrations in the range 2.0 x 10(-7) to 2.0 x 10(-4) mol L(-1) for both GSH and L-Cys, with detection limits of 1.2 x 10(-7) and 1.0 x 10(-7) mol L(-1), respectively. Coupled with microdialysis sampling, the HPLC-ECD system has been successfully used to assess the GSH and L-Cys content of rat striatum.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China. liumeichuan@sohu.com
authors.forename: Meichuan
authors.initials: M
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
authors.forename: Chenglin
authors.initials: C
authors.lastname: Zhang
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000818:Animals; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D003545:Cysteine / Q000032:analysis / Q000737:chemistry; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D017551:Microdialysis; D036103:Nanotechnology; D010084:Oxidation-Reduction; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013997:Time Factors
chemical_list: D005295:Ferrocyanides; D013438:Sulfhydryl Compounds; C116383:ferrocyn; D003035:Cobalt; D005978:Glutathione; D003545:Cysteine