title: Superheated water chromatography-nuclear magnetic resonance spectroscopy and mass spectrometry of vitamins.
abstract: The water-soluble vitamins, pyridoxine, riboflavin, and thiamine, were separated by reversed-phase liquid chromatography using hot or superheated water as the mobile phase and were detected using a range of detectors, including ultraviolet and fluorescence spectroscopy and mass spectrometry. By using deuterium oxide as the eluent, direct on-line nuclear magnetic resonance spectra could be obtained with minimal spectral interference from the mobile phase. Some of the compounds showed deuterium exchange of alkyl-protons when separated at high temperatures.
authors.affiliation: Department of Chemistry, Loughborough University, Loughborough, Leics LE11 3TU, UK.
authors.forename: Orapin
authors.initials: O
authors.lastname: Chienthavorn
authors.forename: Roger M
authors.initials: RM
authors.lastname: Smith
authors.forename: Shikha
authors.initials: S
authors.lastname: Saha
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.forename: Brian
authors.initials: B
authors.lastname: Wright
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Taylor
authors.forename: Eva M
authors.initials: EM
authors.lastname: Lenz
mesh_terms: D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D014815:Vitamins / Q000032:analysis*; D014867:Water / Q000032:analysis*
chemical_list: D014815:Vitamins; D014867:Water