Dimethyl sulfoxide and ebselen prevent convulsions induced by 5-aminolevulinic acid.
We investigated whether intrastriatal (i.s.) administration of 5-aminolevulinic acid (ALA) induces oxidative damage and whether behavioral alterations induced by i.s. administration of ALA could be affected by antioxidants. Unilateral injection of ALA (6 micromol/striatum) increased (approximately 30%) thiobarbituric acid-reactive substances (TBARS), but did not affect striatal content of total thiol groups. ALA-induced body asymmetry was not prevented by pretreatment with ascorbic acid (100 mg/kg, s.c.), dimethyl sulfoxide (DMSO, 0.5 microl/striatum, i.s.) or ebselen (10 nmol/striatum, i.s.). ALA-induced convulsions were not prevented by ascorbic acid, but were partially prevented by DMSO and completely prevented by ebselen. Ebselen completely prevented the increase of striatal TBARS induced by ALA. Results obtained suggest the involvement of reactive species in ALA-induced convulsions and may be of value in understanding the physiopathology of neurological dysfunctions associated to ALA overload.
Prauchner
Carlos André
CA
Curso de Pós-Graduação em Bioquimica Toxicológia, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil.
Agostini
Adriano Neujahr
AN
Morimoto
Akemi
A
Augusti
Paula Rossini
PR
Unfer
Taís Cristina
TC
Zeni
Gilson
G
Mello
Carlos Fernando
CF
Emanuelli
Tatiana
T
D000622:Aminolevulinic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D001393:Azoles / Q000627:therapeutic use*; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D054833:Isoindoles; D008297:Male; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*
D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; C042986:ebselen; D000622:Aminolevulinic Acid; D004121:Dimethyl Sulfoxide