title: Genomic organization of the 8p11-p12 amplicon in three breast cancer cell lines.
abstract: Amplification of chromosomal regions leads to an increase of DNA copy number and expression of oncogenes in human breast cancer (HBC). Amplification of the 8p11-p12 region occurs in 10-15% of primary, uncultured HBCs. In our panel of 11 breast cancer cells, three cell lines, SUM-44, SUM-52, and SUM-225, have overlapping amplicons in the 8p11-p12 region. To characterize genome structure of the amplified regions, we performed fluorescence in situ hybridization using 8p11-p12 BAC clones in the 3 cell lines. The results revealed that the 8p11-p12 amplicon has a highly complex structure and that FGFR1 is not in the common core-amplified domain in 3 breast cancer cell lines with the amplicon. These 3 cell lines provide good models for genetic and functional studies of candidate oncogenes of the 8p11-p12 region.
authors.affiliation: Department of Radiation Oncology, University of Michigan Medical School, 7312 CCGC, PO Box 0948, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0948, USA.
authors.forename: Zeng-Quan
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Donna
authors.initials: D
authors.lastname: Albertson
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Ethier
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002898:Chromosomes, Human, Pair 8; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization