Characterization of synectin expression and promoter activity.
Synectin, a PDZ2 protein, binds to a number of plasma membrane receptors. We characterized synectin promoter and carried out a detailed expression survey. The protein is widely distributed in adult cells and tissues. Expression in the mouse embryo is apparent at day 4.5, peaks at day 12.5 and declines to its adult levels by day 18.5. The level of synectin transcription was minimally affected by exposure to cytokines, growth factors or hypoxia. In summary, synectin's early appearance, constant nature, and ubiquitous distribution suggest that it may be a key structural protein involved in mediation of outside-in signaling.
Zhang
Yufeng
Y
Angiogenesis Research Center, Department of Medicine and Pharmacology, Dartmouth Hitchcock Medical Center, One Medical Center drive, Lebanon, NH 03756, USA.
Chittenden
Thomas
T
Simons
Michael
M
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D015687:Cell Hypoxia / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D016207:Cytokines / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013997:Time Factors; D024363:Transcription Initiation Site; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D016207:Cytokines; C115271:Gipc1 protein, mouse; D006133:Growth Substances; D009479:Neuropeptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D004247:DNA; D008156:Luciferases