Semicarbazide-sensitive amine oxidase in transgenic mice with diabetes.
Semicarbazide-sensitive amine oxidase (SSAO) activity in plasma is increased in diabetes, and in particular, in diabetic patients with vascular complications. It has been speculated that SSAO is involved in the development of such complications due to the production of cytotoxic compounds. In this work, we have induced diabetes in a previously described mouse-model, overexpressing SSAO in smooth muscle cells. SSAO activity was estimated as well as expression of the endogenous mouse gene and human transgene using real-time PCR. Diabetes induced an increase in SSAO activity in serum, kidney, and adipose tissue of transgenic animals. An inverse correlation between SSAO activity and mouse SSAO mRNA levels was observed in transgenic animals with diabetes. These results further support the suggestion of a negative feedback control of the SSAO gene expression. The increased SSAO activity in diabetes is most likely dependent on post-transcriptional modifications or activation of existing inactive enzyme molecules.
Gokturk
C
C
Section of Pharmacology, Department of Neuroscience, Uppsala University, Box 593, Biomedicum, 751 24 Uppsala, Sweden. camilla.gokturk@neuro.uu.se
Nordquist
J
J
Sugimoto
H
H
Forsberg-Nilsson
K
K
Nilsson
J
J
Oreland
L
L
D000273:Adipose Tissue / Q000201:enzymology; D000496:Alloxan; D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D008822:Mice, Transgenic / Q000378:metabolism*; D009130:Muscle, Smooth / Q000201:enzymology*; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000378:metabolism; D014018:Tissue Distribution
D015815:Cell Adhesion Molecules; D011994:Recombinant Proteins; D000496:Alloxan; D006631:Amine Oxidase (Copper-Containing); C492678:semicarbazide-sensitive amine oxidase-vascular adhesion protein-1, mouse