Loktanella hongkongensis sp. nov., a novel member of the alpha-Proteobacteria originating from marine biofilms in Hong Kong waters.
A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009P(T)) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009P(T) and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65.9-66.2 mol%) and fatty acid (86.22-88.52 % 18 : 1omega7c) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009P(T) within the Rhodobacter group of the alpha-subclass of the Proteobacteria. The nearest neighbours belong to the genus Loktanella, with similarity values ranging from 94.5 to 95.5 %. Data on G+C and fatty acid contents support the affiliation to the genus Loktanella. UST950701-009P(T) and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2.0-14.0 %). Both grow in pH 5.0-10.0 and at 8-44 degrees C. Both are positive in oxidase, catalase and beta-galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009P(T) constitutes a novel species within the genus Loktanella. The name Loktanella hongkongensis sp. nov. is proposed; the type strain is UST950701-009P(T) (=NRRL B-41039(T)=JCM 12479(T)) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).
Lau
Stanley C K
SCK
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Tsoi
Mandy M Y
MMY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Li
Xiancui
X
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Plakhotnikova
Ioulia
I
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Wu
Madeline
M
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Wong
Po-Keung
PK
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR.
Qian
Pei-Yuan
PY
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D006723:Hong Kong; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009068:Movement; D010088:Oxidoreductases / Q000032:analysis; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D012462:Saline Solution, Hypertonic; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D014871:Water Microbiology; D001616:beta-Galactosidase / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D010088:Oxidoreductases; D002374:Catalase; D001616:beta-Galactosidase; D005840:Gentian Violet