Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water.
Two mesophilic anaerobic bacterial strains (Z7(T) and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0.2-0.4x4.0-8.0 microm). Growth of the strains was observed at 20-42 degrees C and pH 5.0-7.5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC(15 : 0) (42.83 %) and isoC(14 : 0) (32.11 %). The DNA G+C contents of strains Z7(T) and Z1 were 50.4 and 48.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7(T) (=JCM 12359(T)=AS 1.5012(T)) as the type strain.
Chen
Shuangya
S
Graduate School of the Chinese Academy of Sciences, Beijing 100080, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Dong
Xiuzhu
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004929:Esculin / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D005780:Gelatin / Q000378:metabolism; D020459:Genes, rRNA; D005840:Gentian Violet; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002245:Carbon Dioxide; D004929:Esculin; D000431:Ethanol; D006859:Hydrogen; D005780:Gelatin; D005840:Gentian Violet; D019342:Acetic Acid