The effects of a bradykinin B2 receptor antagonist in extended liver resection with ischemia in dogs.
OBJECTIVE
In liver surgery, total clamping of the portal triad (Pringle's procedure) is commonly used, and sometimes causes liver failure. This study evaluated the effects of a bradykinin B2 receptor antagonist, FR173657 (FR), on ischemia-reperfusion injury during liver resection in dogs.


METHODS
Experimental animals were divided into two groups. In the FR group (n=6), FR (100 nmol/kg/hr) was administered continuously via the portal vein from 30 min before the onset of ischemia until 2 hr after reperfusion. In the control group (n=6), vehicle was injected in the same manner. The right portal pedicle was clamped for 60 min, while the left portal branch was left patent to avoid portal congestion. Following reperfusion, the non-ischemic lobes were resected, and remnant liver function was evaluated.


RESULTS
AST and ALT were significantly (p<0.05) lower in the FR group than in the control group. Hepatic tissue blood flow 30 min after reperfusion was significantly (p<0.05) higher in the FR group than in the control group. Histological tissue damage was mild, and polymorphonuclear neutrophil infiltration was significantly (p<0.05) reduced in the FR group compared with the control group.


CONCLUSIONS
A bradykinin B2 receptor antagonist ameliorated the ischemia-reperfusion injury caused by Pringle's procedure during extended liver resection.
Totsuka
Osamu
O
Second Department of Surgery, Gunma University School of Medicine, Maebashi, Gunma, Japan.
Takeyoshi
Izumi
I
Tsutsumi
Hirofumi
H
Hashimoto
Naoki
N
Sunose
Yutaka
Y
Tokumine
Masahiko
M
Ohwada
Susumu
S
Matsumoto
Koshi
K
Morishita
Yasuo
Y
D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D006498:Hepatectomy / Q000009:adverse effects* / Q000379:methods; D007150:Immunohistochemistry; D007262:Infusions, Intravenous; D007430:Intraoperative Care; D007511:Ischemia / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000098:blood supply*; D008102:Liver Circulation / Q000187:drug effects; D008297:Male; D011336:Probability; D011897:Random Allocation; D043782:Receptor, Bradykinin B2 / Q000008:administration & dosage*; D012016:Reference Values; D015427:Reperfusion Injury / Q000517:prevention & control*; D012307:Risk Factors; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D043782:Receptor, Bradykinin B2