Mahella australiensis gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from an Australian oil well.
A novel Gram-positive, anaerobic and moderately thermophilic bacterium, strain 50-1 BON(T), was isolated from an Australian terrestrial oil reservoir. Cells were spore-forming straight rods, motile by peritrichous flagella. The optimum growth conditions were 50 degrees C, pH 7.5 and 0.1 % NaCl. Strain 50-1 BON(T) fermented arabinose, cellobiose, fructose, galactose, glucose, mannose, sucrose, xylose and yeast extract. Glucose was fermented mainly into lactate, formate, hydrogen and CO(2). The major end product of pyruvate fermentation was acetate together with H(2) and CO(2). Thiosulfate, sulfate, elemental sulfur and nitrate were not used as terminal electron acceptors. The DNA G+C content was 55.5 mol%. The closest phylogenetic relative of strain 50-1 BON(T) was Thermoanaerobacterium thermosulfurigenes (16S rRNA gene sequence similarity of 85.7 %). As strain 50-1 BON(T) was physiologically and phylogenetically different from members of the order 'Thermoanaerobacteriales', it is proposed that strain 50-1 BON(T) (=DSM 15567(T)=CIP 107919(T)) be classified as the type strain of a novel species of a new genus, Mahella australiensis gen. nov., sp. nov.
Bonilla Salinas
Monica
M
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Fardeau
Marie-Laure
ML
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Thomas
Pierre
P
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Cayol
Jean-Luc
JL
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
Patel
Bharat K C
BKC
School of Biomolecular and Biomedical Sciences, Griffith University, Brisbane, Australia.
Ollivier
Bernard
B
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
D000693:Anaerobiosis; D001315:Australia; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D005407:Flagella; D005561:Formates / Q000378:metabolism; D020459:Genes, rRNA; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012988:Soil Microbiology; D013457:Sulfur Compounds / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005561:Formates; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013457:Sulfur Compounds; C030544:formic acid; D002245:Carbon Dioxide; D019344:Lactic Acid; D012965:Sodium Chloride; D006859:Hydrogen