Listeria monocytogenes infection-dependent transfer of exogenously added DNA to fibroblast COS-1 cells.
The addition of double-stranded circular or linear DNA encoding EGFP (the Enhanced Green Fluorescent Protein) to a Listeria -containing infection medium resulted in up to 8.6% COS-1 cells expressing the reporter protein. The transfer of naked DNA into host cells upon infection by Listeria was found to be dependent on the ability of the bacteria to synthesize internalins and listeriolysin. Since no binding of DNA to the bacterial cells was detected, DNA uptake seems to be the consequence of the simultaneous entry of infection medium, and thus of naked DNA, via the phagosomes induced by the bacterium to facilitate its own entry into the host cells.
Stritzker
J
J
Lehrstuhl für Mikrobiologie, Biozentrum, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany. j-stritzker@biozentrum.uni-wuerzburg.de
Goebel
W
W
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001427:Bacterial Toxins / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006460:Hemolysin Proteins; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D016296:Mutagenesis; D010587:Phagocytosis
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C485184:enhanced green fluorescent protein; C069761:internalin protein, Bacteria; D049452:Green Fluorescent Proteins; D004247:DNA; C038705:hlyA protein, Listeria monocytogenes