RGS14 is a mitotic spindle protein essential from the first division of the mammalian zygote.
Heterotrimeric G protein alpha subunits, RGS proteins, and GoLoco motif proteins have been recently implicated in the control of mitotic spindle dynamics in C. elegans and D. melanogaster. Here we show that "regulator of G protein signaling-14" (RGS14) is expressed by the mouse embryonic genome immediately prior to the first mitosis, where it colocalizes with the anastral mitotic apparatus of the mouse zygote. Loss of Rgs14 expression in the mouse zygote results in cytofragmentation and failure to progress to the 2-cell stage. RGS14 is found in all tissues and segregates to the nucleus in interphase and to the mitotic spindle and centrioles during mitosis. Alteration of RGS14 levels in exponentially proliferating cells leads to cell growth arrest. Our results indicate that RGS14 is one of the earliest essential product of the mammalian embryonic genome yet described and has a general role in mitosis.
Martin-McCaffrey
Luke
L
Department of Physiology and Pharmacology, Schulich School of Medicine, University of Western Ontario, London, Ontario N6A 5C1, Canada.
Willard
Francis S
FS
Oliveira-dos-Santos
Antonio J
AJ
Natale
David R C
DR
Snow
Bryan E
BE
Kimple
Randall J
RJ
Pajak
Agnieszka
A
Watson
Andrew J
AJ
Dagnino
Lina
L
Penninger
Josef M
JM
Siderovski
David P
DP
D'Souza
Sudhir J A
SJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology; D002455:Cell Division / Q000502:physiology*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D017353:Gene Deletion; D005822:Genetic Vectors; D006367:HeLa Cells; D006579:Heterozygote; D006801:Humans; D006834:Hydrazines; D008322:Mammals / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D008941:Spindle Apparatus / Q000737:chemistry*; D015053:Zygote / Q000502:physiology*
C417561:Alexa 488 hydrazide; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D006834:Hydrazines; D020710:RGS Proteins; D034741:RNA, Small Interfering; C491399:Rgs14 protein, mouse; C106374:Rgs14 protein, rat; D016650:Fluorescein-5-isothiocyanate