title: Immunotherapy with plasmid DNA encoding mycobacterial hsp65 in association with chemotherapy is a more rapid and efficient form of treatment for tuberculosis in mice.
abstract: Tuberculosis (TB) remains a threat for public health, killing around 3 million people a year. Despite the fact that most cases can be cured with antibiotics, the treatment is long and patients relapse if chemotherapy is not continued for at least 6 months. Thus, a better characterization of the working principles of the immune system in TB and identification of new immunotherapeutic products for the development of shorter regimens of treatment are essential to achieve an effective management of this disease. In the present work, we demonstrate that immunotherapy with a plasmid DNA encoding the Mycobacterium leprae 65 kDa heat-shock protein (hsp65) in order to boost the efficiency of the immune system, is a valuable adjunct to antibacterial chemotherapy to shorten the duration of treatment, improve the treatment of latent TB infection and be effective against multidrug-resistant bacilli (MDR-TB). We also showed that the use of DNA-hsp65 alone or in combination with other drugs influence the pathway of the immune response or other types of inflammatory responses and should augment our ability to alter the course of immune response/inflammation as needed, evidencing an important target for immunization or drug intervention.
authors.affiliation: REDE-TB, Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: C L
authors.initials: CL
authors.lastname: Silva
authors.forename: V L D
authors.initials: VL
authors.lastname: Bonato
authors.forename: A A M
authors.initials: AA
authors.lastname: Coelho-Castelo
authors.forename: A O
authors.initials: AO
authors.lastname: De Souza
authors.forename: S A
authors.initials: SA
authors.lastname: Santos
authors.forename: K M
authors.initials: KM
authors.lastname: Lima
authors.forename: L H
authors.initials: LH
authors.lastname: Faccioli
authors.forename: J M
authors.initials: JM
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics*; D003131:Combined Modality Therapy; D015316:Genetic Therapy / Q000379:methods*; D016233:Immunotherapy, Active / Q000379:methods*; D051379:Mice; D023421:Models, Animal; D014376:Tuberculosis / Q000188:drug therapy / Q000628:therapy*; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D019444:Vaccines, DNA; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins