[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].
OBJECTIVE
To observe the method of fluorescent-polymerase chain reaction (F-PCR) and gene-hip technique in detecting coronavirus in severe acute respiratory syndrome (SARS) and its value for clinical application.


METHODS
Serum of 60 SARS patients, 20 samples of serum and 20 samples of gargling fluid of medical staff of the "Fever Clinic", and one cDNA specimen obtained from one SARS suspect patient were examined with F-PCR diagnosis kit and gene-chip technique for SARS coronavirus.


RESULTS
Three methods, including DR Chip and two F-PCR kit from Zhongshan College Da'an gene stock company and Shanghai Fortune industrial joint-stock Co, Ltd, were used, and the results were all negative for all specimens, except one cDNA specimen, which was obtained from one SARS suspect patient, virus could be amplified by F-PCR.


CONCLUSIONS
Special RNA fragment of SARS virus has not been detected in gargling fluid and serum of SARS convalescents and medical staff.
Jia
Xing-wang
XW
Department of Clinical Biochemistry, PLA General Hospital and Postgraduate Medical School, Beijing 100853, China. jiaxingw301@yahoo.com.cn
Tian
Ya-ping
YP
Shen
Hong
H
Song
Shu-zhen
SZ
Yuan
Bin
B
Deng
Xin-xin
XX
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003289:Convalescence; D005260:Female; D006282:Health Personnel; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D055815:Young Adult
D012367:RNA, Viral