Manganese peroxidase of Agaricus bisporus: grain bran-promoted production and gene characterization.
The main manganese peroxidase (MnP) isoenzyme of Agaricus bisporus ATCC 62459 produced in lignocellulose-containing cultures was isolated, cloned and sequenced. In liquid medium, where MnP was previously detected only in trace amounts, the production of MnP was enhanced by rye and wheat bran supplements. The pI (3.25) and N-terminal amino acid sequence (25 aa) of the enzyme from bran-containing cultures were identical to those reported from compost-isolated MnP1. MnP1 is a 328-aa long polypeptide preceded by a 26-aa leader peptide. The nucleotide sequence and putative amino acid sequence of MnP1 reveal its similarity to Pleurotus ostreatus MnP3 (62.5%), Lepista irina versatile peroxidase (VP) (61.8%) and Pleurotus eryngii VPs VPL2 and VPL1 (61.9% and 61.2%, respectively). The intron-exon structure resembles that of P. ostreatus MnP1 and P. eryngii VPL1. Despite the sequence similarity to VPs, in the A. bisporus MnP1 sequence, alanine (A163) is present instead of tryptophane (W164), distinguishing it from the veratryl alcohol oxidising P. eryngii VPLs. The MnP sequence can be used as a tool to examine the pattern of ligninolytic gene expression during the growth and fruiting of A. bisporus to optimise compost composition, fungal growth and mushroom production.
Lankinen
Pauliina
P
Department of Applied Chemistry and Microbiology, Viikki, Biocenter 1, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland. pauliina.lankinen@helsinki.fi
Hildén
Kristiina
K
Aro
Nina
N
Salkinoja-Salonen
Mirja
M
Hatakka
Annele
A
D000364:Agaricus / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004043:Dietary Fiber; D005091:Exons; D005800:Genes, Fungal; D007438:Introns; D042845:Laccase / Q000378:metabolism; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics*
D003470:Culture Media; D018076:DNA, Complementary; D004271:DNA, Fungal; D004043:Dietary Fiber; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase