A database search for double-strand containing RNAs in Dictyostelium discoideum.
In eukaryotic cells, double-stranded RNA is degraded to 21mers and triggers RNA interference. Using a pattern description language, we have searched the EMBL database for sequences with the potential to form double strands in cis in Dictyostelium discoideum. No extended inverted repeats were found in mRNAs. However, the antisense direction of some mRNAs encoding regulatory or developmentally regulated proteins showed the ability to form double-stranded regions. In EST archives, we found potential double strands derived from a few genes, but these transcripts are not continuously encoded in the genome. Most likely, they represent hybrid molecules of sense and antisense RNAs.
Gräf
Stefan
S
Institut für Physikalische Biologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany.
Borisova
Branimira E
BE
Nellen
Wolfgang
W
Steger
Gerhard
G
Hammann
Christian
C
D000818:Animals; D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D004023:Dictyostelium / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D016053:RNA, Protozoan / Q000737:chemistry*
D012330:RNA, Double-Stranded; D016053:RNA, Protozoan