title: Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy.
abstract: Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.
authors.affiliation: Department of Chemistry, Hanyang University, Ansan 426-791, Korea.
authors.forename: Taehan
authors.initials: T
authors.lastname: Park
authors.forename: Moonkwon
authors.initials: M
authors.lastname: Lee
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Yang S
authors.initials: YS
authors.lastname: Kim
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Duck Joong
authors.initials: DJ
authors.lastname: Kim
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D019840:2-Propanol / Q000032:analysis / Q000737:chemistry; D004129:Dimethylpolysiloxanes; D000431:Ethanol / Q000032:analysis / Q000737:chemistry; D008832:Microchemistry / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D012828:Silicones; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; D000431:Ethanol; C013830:baysilon; D019840:2-Propanol