Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora.
An actinomycete that developed sporangia containing four spores in a single row at the ends of short sporangiophores on branched aerial hyphae was isolated from subtropical forest soil. The isolate contained menaquinone MK-9(H(4)), glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids and madurose in the whole-cell hydrolysate. The 16S rRNA gene sequence indicated that the isolate formed a monophyletic cluster with Planotetraspora mira. On the basis of morphological and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species of the genus Planotetraspora is proposed, Planotetraspora silvatica sp. nov. (type strain, TT 00-51(T)=NBRC 100141(T)=DSM 44746(T)).
Tamura
Tomohiko
T
Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0812, Japan.
Sakane
Takeshi
T
Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0812, Japan.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D000409:Alanine / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D003960:Diaminopimelic Acid / Q000302:isolation & purification; D020459:Genes, rRNA; D018698:Glutamic Acid / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D024482:Vitamin K 2 / Q000031:analogs & derivatives* / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D018698:Glutamic Acid; C073355:menaquinone 9; D003960:Diaminopimelic Acid; D000409:Alanine