Determination of polyethylene glycols of different molecular weight in the stratum corneum.
We developed a sensitive method for determination of polyethylene glycols (PEGs) of different molecular weight (MW) in the human stratum corneum (SC) obtained by tape stripping. The analysis is based on derivatization with pentafluoropropionic anhydride (PFPA) and gas chromatography-electron capture detection (GC-ECD). The identification and quantification of PEGs was done using individual oligomers. The method showed to be suitable for studying permeability in normal and impaired skin with respect to MW in the range of 150-600 Da.
Jakasa
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Coronel Institute for Occupational and Environmental Health, Academic Medical Center, University of Amsterdam, P.O. Box 22700, NL-1100 DE Amsterdam, The Netherlands. i.jakasa@amc.uva.nl
Calkoen
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Kezic
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D000293:Adolescent; D000328:Adult; D005260:Female; D005466:Fluorocarbons / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010539:Permeability; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry* / Q000378:metabolism
D005466:Fluorocarbons; C068048:pentafluoropropionic anhydride; D011092:Polyethylene Glycols