title: Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots.
abstract: Gram-negative bacteria were isolated from knots induced by Pseudomonas savastanoi in olive trees (Olea europaea L.). A total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. Biochemical characterization indicated that all the strains belong to the family Enterobacteriaceae. Phylogenetic analyses of the 16S rRNA genes of these novel isolates revealed that they formed a homogeneous cluster within Erwinia species. DNA signatures of these isolates were identical to those described for the genus Erwinia. The strains formed a homogeneous group as shown by DNA-DNA hybridization analysis and numerical analysis of phenotypic data, clearly differentiated from all species of Erwinia with validly published names. The data provide strong evidence of the differentiation of these strains from the most closely related species. Therefore, these isolates represent a novel species, for which the name Erwinia toletana sp. nov. is proposed. The isolates are available at CFBP, CECT and ATCC. The G+C content is 52+/-0.5 mol%. The type strain is CFBP 6631(T) (=A37(T)=ATCC 700880(T)=CECT 5263(T)).
authors.affiliation: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Rojas
authors.affiliation: Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
authors.forename: Jose Esteban García de Los
authors.initials: JEGL
authors.lastname: Rios
authors.affiliation: UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
authors.forename: Marion Fischer-Le
authors.initials: MF
authors.lastname: Saux
authors.affiliation: Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Jimenez
authors.affiliation: Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Reche
authors.affiliation: UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bonneau
authors.affiliation: UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Sutra
authors.affiliation: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
authors.forename: Françoise
authors.initials: F
authors.lastname: Mathieu-Daudé
authors.affiliation: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: McClelland
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004885:Erwinia / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D031658:Olea / Q000382:microbiology*; D010619:Phenazines; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011549:Pseudomonas / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet