Enzymatic cross-linking of a phenolic polymer extracted from the marine alga Fucus serratus.
We have shown that a phenolic polymer (PP) extracted from Fucus serratus can be cross-linked using a vanadium-dependent bromoperoxidase (BPO). The methanol extracted PP was adsorbed to a quartz crystal sensor and the cross-linking was initiated by the addition of BPO, KBr, and H2O2. The decreased dissipation upon addition of the cross-linking agents, as measured with the quartz crystal microbalance with dissipation monitoring (QCM-D) method, was interpreted as intramolecular cross-links were formed between different phloroglucinol units in the PP. With surface plasmon resonance, it was shown that no desorption occurred from the sensor surface during the cross-linking. UV/vis spectroscopy verified the results achieved with QCM-D that all components, i.e., BPO, KBr, and H2O2, were necessary in order to achieve intramolecular oxidative cross-linking of the polymer.
Berglin
Mattias
M
Cell and Molecular Biology, Interface Biophysics, Göteborg University, Box 462, SE-405 30 Sweden. mattias.berglin@gmm.gu.se
Delage
Ludovic
L
Potin
Philippe
P
Vilter
Hans
H
Elwing
Hans
H
D000327:Adsorption; D001672:Biocompatible Materials; D001965:Bromides / Q000737:chemistry; D002845:Chromatography; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D044645:Fucus / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D010544:Peroxidases / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D013053:Spectrophotometry; D020349:Surface Plasmon Resonance; D013997:Time Factors; D000267:Tissue Adhesions; D014466:Ultraviolet Rays; D014639:Vanadium / Q000494:pharmacology
D001672:Biocompatible Materials; D001965:Bromides; D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D010636:Phenols; D011108:Polymers; D017680:Potassium Compounds; D014639:Vanadium; D019800:Phenol; D006861:Hydrogen Peroxide; D010544:Peroxidases; C026195:bromide peroxidase; C039004:potassium bromide; D000432:Methanol