Microarray method to monitor 40 intestinal bacterial species in the study of azo dye reduction.
Azo dyes are widely used in dye manufacturing, paper printing, textile industries, and as tattoo pigmentation. Since intestinal and skin bacteria can metabolize certain azo dyes to carcinogenic compounds, many researchers have studied the azoreductases of these bacteria. In this study, we used a microarray method to identify the intestinal bacterial species from cultured fecal samples in Brain Heart Infusion (BHI) broth with or without azo dyes that may be involved in azo dye reduction. The microarray was designed to identify 40 bacterial species that are reported in the literature to be predominant in human feces. Results from this study showed 26-30 species are present in the cultured fecal samples. The representative bacteria were then examined for the azo dye reduction activity.
Wang
Rong-Fu
RF
Microbiology Division, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA. rwang@nctr.fda.gov
Chen
Huizhong
H
Paine
Donald D
DD
Cerniglia
Carl E
CE
D001391:Azo Compounds / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D010084:Oxidation-Reduction; D013045:Species Specificity
D001391:Azo Compounds; D004396:Coloring Agents