Immunological properties of FMDV-gP64 fusion proteins expressed on SF9 cell and baculovirus surfaces.
In the present report, we characterized the immune response and the protection conferred by recombinant baculoviruses or infected insect cells expressing the fusions gp64-P1 and gp64-site A FMDV antigens. Mice, vaccinated intraperitoneally with gp64-P1 immunogens, showed a low-antibody response and a variable degree of protection. However, when mice received recombinant baculoviruses or infected insect cells expressing the fusion protein gp64-site A, high-ELISA and seroneutralizing titers (SNT) against FMDV were elicited. All mice immunized with Sf9 cells expressing FMDV site A developed a protective immune response against challenge with virulent FMDV, indicating that the baculovirus display of foreign epitopes is a promising approach to biosynthetic vaccines.
Tami
Cecilia
C
Instituto de Biotecnología, CICVyA, INTA, Castelar, CC25 (1712), Buenos Aires, Argentina.
Peralta
Andrea
A
Barbieri
Rosario
R
Berinstein
Analía
A
Carrillo
Elisa
E
Taboga
Oscar
O
D000086042:3C Viral Proteases; D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D018411:Spodoptera / Q000166:cytology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases