title: Molecular characteristics of strains of the cameroon family, the major group of Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis.
abstract: A preliminary investigation of the genetic biodiversity of Mycobacterium tuberculosis complex strains in Cameroon, a country with a high prevalence of tuberculosis, described a group of closely related M. tuberculosis strains (the Cameroon family) currently responsible for more than 40% of smear-positive pulmonary tuberculosis cases. Here, we used various molecular methods to study the genetic characteristics of this family of strains. Cameroon family M. tuberculosis strains (i) are part of the major genetic group 2 and lack the TbD1 region like other families of epidemic strains, (ii) lack spacers 23, 24, and 25 in their direct repeat (DR) region, (iii) have an identical number of repeats in 8 of 12 variable-number tandem repeats of mycobacterial interspersed repetitive unit (MIRU-VNTR) loci, (iv) have similar IS6110-restriction fragment length polymorphism (RFLP) multiband patterns (10 to 15 copies) with seven common IS6110 bands, (v) do not have an IS6110 element in their DR locus, and (vi) have four IS6110 elements in open reading frames (adenylate cyclase, phospholipase C, moeY, and ATP binding genes). Analysis by spoligotyping, MIRU-VNTR, and IS6110-RFLP typing methods revealed differences not observed in previous studies; polymorphism as assessed by MIRU-VNTR typing was lower than suggested by spoligotyping, and in rare cases, strains with identical IS6110-RFLP patterns had spoligotypes differing by as much as 15 spacers. Our findings confirm the recent expansion of this family in Cameroon and indicate that the interpretation of molecular typing results has to be adapted to the characteristics of the strain population within each setting. The knowledge of this particular genotype, with its large involvement in tuberculosis in Cameroon, allows greater refinement of tuberculosis transmission studies by interpreting data in the context of this geographic area.
authors.affiliation: Centre National de Référence des Mycobactéries, Institut Pasteur, 25 rue du Dr Roux, 75724 Cedex 15, Paris, France.
authors.forename: Sara Ngo
authors.initials: SN
authors.lastname: Niobe-Eyangoh
authors.forename: Christopher
authors.initials: C
authors.lastname: Kuaban
authors.forename: Philippe
authors.initials: P
authors.lastname: Sorlin
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Thonnon
authors.forename: Veronique
authors.initials: V
authors.lastname: Vincent
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Gutierrez
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002163:Cameroon / Q000453:epidemiology; D004251:DNA Transposable Elements; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D016254:Mutagenesis, Insertional; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D005819:Genetic Markers; D009841:Oligonucleotides