title: Microsatellite mutation directed by an external stimulus.
abstract: Microsatellites are regions of DNA containing tandem repeats of a core 2-6 bp nucleotide sequence. To test the hypothesis that microsatellite mutation can be directed by exposure to specific external cues, control and treatment groups of resistant and susceptible wheat varieties were grown under controlled conditions and genotyped at a number of microsatellite loci that map to chromosomes known to contain Fusarium head blight (FHB) resistance/susceptibility loci. Genotyping was undertaken both prior to and following exposure to Fusarium graminearum, the FHB pathogen. Within a month of inoculation of inflorescences, 58% of experimental plants, and no control plants, had acquired a novel allele at the locus Xgwm112.1. This allele was detected only in head blight affected tissue. Uninoculated control plants, and leaf samples from inoculated plants, showed no mutation. Cloning and sequencing of PCR products indicates that the new allele was generated by contraction of the (CT)(n) repeat motif. Observation of the same deletion-based mutation in all varieties, its absence in control plants not exposed to the head blight pathogen, and the detection of no similar mutational events in a control panel of loci not expected to show mutation, indicates that this example of microsatellite mutation is induced and/or caused by FHB infection.
authors.affiliation: CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Qld 4067, Australia. adele.schmidt@csiro.au
authors.forename: Adele L
authors.initials: AL
authors.lastname: Schmidt
authors.forename: Vivek
authors.initials: V
authors.lastname: Mitter
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005670:Fusarium; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D017931:DNA Primers