title: Liquid chromatography--electrospray ionisation mass spectrometry method for the determination of escitalopram in human plasma and its application in bioequivalence study.
abstract: A novel liquid chromatographic--electrospray ionisation mass spectrometric (LC--ESI-MS) method has been developed for the determination of escitalopram, an antidepressant in human plasma using paroxetine as internal standard. The method involved liquid--liquid extraction of the analyte from human plasma with a mixture of diethyl ether and dichloromethane (70:30, v/v). The chromatographic separation was achieved within 7.0 min by using 2.0 mM ammonium acetate (pH 5.0)--acetonitrile (54:46, v/v) as mobile phase and a ODS YMCAQ 150 mm x 4.6 mm analytical column; the flow-rate was 1.0 ml/min. Ion signals m/z 325.0 and 330.0 for escitalopram and internal standard, were measured in the positive mode. A detailed validation of the method was performed as per USFDA guidelines and the standard curves were found to be linear in the range of 1.0-200 ng/ml with a mean correlation coefficient more than 0.99. The absolute recovery was more than 75% for both escitalopram and internal standard. The method was simple, sensitive, precise, accurate and was successfully applied to the bioequivalence study of escitalopram in healthy, male, human subjects.
authors.affiliation: Biomedical and DMPK Department, Zydus Research Centre, Sarkhej-Bavla N.H. No. 8A, Moraiya, Ahmedabad 382213, India. sonusingh@zyduscadila.com
authors.forename: Sonu Sundd
authors.initials: SS
authors.lastname: Singh
authors.forename: Hiten
authors.initials: H
authors.lastname: Shah
authors.forename: Sapna
authors.initials: S
authors.lastname: Gupta
authors.forename: Manish
authors.initials: M
authors.lastname: Jain
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Sharma
authors.forename: Purav
authors.initials: P
authors.lastname: Thakkar
authors.forename: Ruchy
authors.initials: R
authors.lastname: Shah
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D015283:Citalopram / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D015283:Citalopram; D017367:Selective Serotonin Reuptake Inhibitors