title: Simple and rapid determination of adenosine in human synovial fluid with high performance liquid chromatography-mass spectrometry.
abstract: A simple, fast, sensitive and selective reversed-phase high performance liquid chromatography-mass spectrometry coupling with an electrospray ionization (ESI) interface method is described for the determination of adenosine in human synovial fluid. This method involved the use of the [M + H](+)ions of adenosine and 2-chloroadenosine (internal standard for the assay) at m/z 268 and 302 in positive ion mode with selective ion monitoring (SIM). Separation was carried out on a 2.0 x 150 mm Shimadzu VP-ODS column by using an isocratic elution with a mobile phase consisting of water (94%),methanol (5%) and formic acid (1%). No interference with the components of the biological matrix was observed in the determination conditions. The calibration curve was linear in the range of 0.2-140 microgml(-1). The limits of quantification (LOQ) and detection (LOD) were 0.2 and 0.03 microgml(-1), respectively. The standard recoveries were between 93.3 and 104.0%. The method was successfully applied to determination of adenosine in some synovial fluids of patients affected by rheumatoid arthritis.
authors.affiliation: College of Chemistry and Chemical Engineering of Central South University, Changsha 410083, P. R. China. fl18huang@yahoo.com.cn
authors.forename: Lan-Fang
authors.initials: LF
authors.lastname: Huang
authors.forename: Fang-Qiu
authors.initials: FQ
authors.lastname: Guo
authors.forename: Yi-Zeng
authors.initials: YZ
authors.lastname: Liang
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Li
authors.forename: Ben-Mei
authors.initials: BM
authors.lastname: Cheng
mesh_terms: D000241:Adenosine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D000241:Adenosine