Efficient purification of bromoviruses by ultrafiltration.
Ultrafiltration using polyethersulfone-membranes was evaluated as an efficient and preferred method for purifying Cowpea Chlorotic Mottle Virus (CCMV). Cesium chloride (CsCl) ultracentrifugation and ultrafiltration protocols are described, and comparative UV-spectroscopic and electron micrograph results are presented. CCMV purified by ultrafiltration are shown to be equivalent to CCMV purified by ultracentrifugation, while reducing purification time by two days and avoiding the need for expensive capital overheads such as ultracentrifuges, rotors and toxic CsCl chemical waste.
Michel
Jean-Philippe
JP
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA.
Gingery
Mari
M
Lavelle
Laurence
L
D017795:Bromovirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002586:Cesium; D002712:Chlorides; D036103:Nanotechnology; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D014462:Ultrafiltration / Q000379:methods*
D002712:Chlorides; D002586:Cesium; C028019:cesium chloride