Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis).
A mapping referential family (F(1)) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.
Watanabe
Tomohisa
T
Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Sendai, Miyagi 981-8555, Japan.
Fujita
Hironobu
H
Yamasaki
Kimio
K
Seki
Shingo
S
Taniguchi
Nobuhiko
N
D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002874:Chromosome Mapping; D018895:Microsatellite Repeats / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D036441:Osmeriformes / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length