Psychrophilicity of Bacillus psychrosaccharolyticus: a proteomic study.
Psychrophilicity of Gram-positive bacterium, Bacillus psychrosaccharolyticus was investigated in a proteomic approach. One hundred and thirty-one protein spots were analyzed by electrospray ionization-quadrupole-time of flight-tandem mass spectrometry and identified using an unpublished translated contig database as well as a nonredundant Gram-positive bacteria protein database from NCBI because of the lack of a genome sequence of this organism. Results focused on proteomic behavior of cold-response show that global up-regulation of metabolic functions and protective mechanism by stress responses might play a major role in psychrophilicity of B. psychrosaccharolyticus.
Seo
Jong Bok
JB
Seoul Branch, Korea Basic Science Institute, Seoul.
Kim
Hye Sook
HS
Jung
Gyoo Yeol
GY
Nam
Myung Hee
MH
Chung
Joo Hee
JH
Kim
Jin Young
JY
Yoo
Jong Shin
JS
Kim
Chan Wha
CW
Kwon
Ohoak
O
D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003080:Cold Temperature; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000378:metabolism; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D020543:Proteome