Combined phospholipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis of bacterial diversity and physiological status in an intertidal microbial mat.
A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.
Villanueva
Laura
L
Department of Microbiology, University of Barcelona, Barcelona, Spain. villanueva@ub.edu
Navarrete
Antoni
A
Urmeneta
Jordi
J
White
David C
DC
Guerrero
Ricardo
R
D001419:Bacteria / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D015373:Bacterial Typing Techniques; D015415:Biomarkers; D018533:Biomass; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005618:Fresh Water / Q000382:microbiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D015415:Biomarkers; D004275:DNA, Ribosomal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S