Porphyrobacter donghaensis sp. nov., isolated from sea water of the East Sea in Korea.
Two Gram-negative, motile, non-spore-forming, bacteriochlorophyll a-containing slightly halophilic strains, SW-132(T) and SW-158, were isolated from sea water of the East Sea in Korea, and subjected to a polyphasic taxonomic study. The two isolates were characterized chemotaxonomically as having Q-10 as the predominant respiratory lipoquinone and major amounts of unsaturated fatty acids C(18 : 1)omega7c and C(17 : 1)omega6c. The DNA G+C contents of the two strains were in the range 66.8-65.9 mol%. The 16S rRNA gene sequences of strains SW-132(T) and SW-158 were 99.9 % (1 nt difference) similar and their mean level of DNA-DNA relatedness was 86 %. The 16S rRNA gene sequence analysis showed that strains SW-132(T) and SW-158 are phylogenetically closely related to Porphyrobacter species and Erythromicrobium ramosum. Similarities between the 16S rRNA gene sequences of strains SW-132(T) and SW-158 and the type strains of Porphyrobacter species and E. ramosum ranged from 97.8 to 99.0 %. DNA-DNA relatedness data indicated that strains SW-132(T) and SW-158 are members of a genomic species that is separate from the four Porphyrobacter species. On the basis of phenotypic and phylogenetic data and genetic distinctiveness, strains SW-132(T) (=KCTC 12229(T)=DSM 16220(T)) and SW-158 (=KCTC 12230) are classified as a novel Porphyrobacter species, for which the name Porphyrobacter donghaensis sp. nov. is proposed.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Lee
Mi-Hwa
MH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D015373:Bacterial Typing Techniques; D025541:Bacteriochlorophyll A / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D007723:Korea; D008969:Molecular Sequence Data; D009068:Movement; D010619:Phenazines; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D013171:Spores, Bacterial / Q000166:cytology; D014871:Water Microbiology
D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D005840:Gentian Violet