title: In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line.
abstract: The effect of classical swine fever (CSF) virus on some phenotypic and functional features of an established porcine aortic endothelial cell (AOC) line was investigated. AOC cells show most of the characteristics of primary endothelial cells, avoiding the alterations and senescence that these cells undergo after a few passages in culture. AOC cells were susceptible to CSF virus infection to a high degree, reaching 90% of CSF virus positive cells after 24 h of infection; however as with other porcine susceptible cells, no cytopathic effect could be observed. In these conditions none of the surface molecules studied, including SLA-II MHC antigens, adhesion or co-stimulatory molecules, were altered by virus infection after 24 or 48 h. Functionally CSF virus infection induced a decrease in the pro-coagulant activity of the AOC cells, determined by the increase in the clot formation time shown by the lysates of these cells. This contrasts with the increase observed in the expression of mRNA corresponding to IL-1 alpha and IL-6, two proinflammatory and pro-coagulant cytokines, in CSF virus-infected AOC cells.
authors.affiliation: CISA-INIA, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Campos
authors.forename: Concepción
authors.initials: C
authors.lastname: Revilla
authors.forename: Sonia
authors.initials: S
authors.lastname: Chamorro
authors.forename: Belén
authors.initials: B
authors.lastname: Alvarez
authors.forename: Angel
authors.initials: A
authors.lastname: Ezquerra
authors.forename: Javier
authors.initials: J
authors.lastname: Domínguez
authors.forename: Fernando
authors.initials: F
authors.lastname: Alonso
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001779:Blood Coagulation Factors / Q000378:metabolism; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis; D042783:Endothelial Cells / Q000378:metabolism* / Q000821:virology*; D005786:Gene Expression Regulation; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine
chemical_list: D001779:Blood Coagulation Factors; D016207:Cytokines; D012333:RNA, Messenger