title: Simultaneous determination of levodopa and benserazide by stopped-flow injection analysis and three-way multivariate calibration of kinetic-spectrophotometric data.
abstract: The simultaneous determination of levodopa and benserazide in pharmaceutical formulations is described, based on the application of multidimensional partial least-squares regression to the kinetic-spectrophotometric data provided by diode-array detection within a stopped-flow injection method where analytes react with periodate. Flow injection parameters were adequately optimized. Accurate analysis is performed with no sample pre-treatment steps, and with minimum experimental effort. Satisfactory recovery results were obtained on a number of synthetic and commercial samples, in the latter case including the comparison with liquid chromatography measurements.
authors.affiliation: Laboratorio FIA-Química Analítica, Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, (B8000CPB) Bahía Blanca, Argentina.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pistonesi
authors.forename: María E
authors.initials: ME
authors.lastname: Centurión
authors.forename: Beatriz S
authors.initials: BS
authors.lastname: Fernández Band
authors.forename: Patricia C
authors.initials: PC
authors.lastname: Damiani
authors.forename: Alejandro C
authors.initials: AC
authors.lastname: Olivieri
mesh_terms: D001545:Benserazide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D007980:Levodopa / Q000032:analysis*; D015999:Multivariate Analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D007980:Levodopa; D001545:Benserazide