title: Simultaneous determination of 2-phenylbenzotriazole-type mutagens, PBTA-1 through -8, in river water by liquid chromatography-tandem mass spectrometry.
abstract: We describe a method for the simultaneous determination of eight kinds of phenylbenzotriazole-type mutagens (PBTA-1, -2, -3, -4, -5, -6, -7 and -8) in river water based on liquid chromatography-tandem mass spectrometry (LC/MS/MS). The application of dopant-assisted atmospheric pressure photoionization (APPI) for the detection of the PBTAs was studied. The APPI technique provided higher PBTA signal intensities than those obtained with an electrospray ionization (ESI) source, and the APPI method was used for the determination of the PBTAs. A solid-phase extraction procedure was used for the extractions of PBTA-1 through -8 from river water. The procedure was rapid and the relative standard deviations were below 15%. The detection limits of PBTA-1 through -8 in river water using the proposed method were found to range from 0.04 to 0.5 ng L(-1) and PBTAs were successfully detected in river water at sub-ng L(-1) levels.
authors.affiliation: Osaka City Institute of Public Health & Environmental Sciences, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. hiroshi.moriwaki@iphes.city.osaka.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Hiroya
authors.initials: H
authors.lastname: Harino
authors.forename: Taro
authors.initials: T
authors.lastname: Yoshikura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohe
authors.forename: Haruo
authors.initials: H
authors.lastname: Nukaya
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Terao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sawanishi
authors.forename: Keiji
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Miyakoda
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Alary
mesh_terms: D002853:Chromatography, Liquid; D004784:Environmental Monitoring / Q000379:methods; D007564:Japan; D013058:Mass Spectrometry / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D045483:Rivers / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: C115463:2-(2-(acetylamino)-4-(N-(2-cyanoethyl)ethylamino)-5-methoxyphenyl)-5-amino-7-bromo-4-chloro-2H-benzotriazole; D009153:Mutagens; D014230:Triazoles; D014874:Water Pollutants, Chemical