[Assessment of the diagnostic value of RT-PCR on amniotic fluid for prenatal diagnosis of congenital rubella infection].
OBJECTIVE
To assess the diagnostic value of RT-PCR on amniotic fluid (AF) for prenatal diagnosis of congenital rubella infection.


METHODS
RT-PCR on AF was compared to specific IgM antibody detection in foetuses and/or newborns in 45 pregnant women with confirmed primary infection.


RESULTS
specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%.


CONCLUSIONS
RT PCR may be considered as a valuable tool for prenatal diagnosis of foetal rubella infection.
Macé
M
M
Service de microbiologie-immunologie biologique, hôpital Antoine-Béclère, 92141 Clamart, France.
Cointe
D
D
Six
C
C
Levy-Bruhl
D
D
Parent du Chatelet
I
I
Ingrand
D
D
Grangeot-Keros
L
L
D000653:Amniotic Fluid / Q000821:virology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011296:Prenatal Diagnosis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000235:genetics* / Q000302:isolation & purification