title: [FRAP, FLIP, FRET, BRET, FLIM, PRIM...new techniques for a colourful life].
abstract: Cell and tissue imaging provides scientists with wonderful tools, thanks to a fruitful dialog between chemistry, optical, mechanical, computational sciences and biology. Confocal microscopy, videomicroscopy together with a new generation of fluorochromes (especially those derived from green fluorescent protein, GFP) and image analysis software allow to visualize life in all its dimensions (space and time). Cell imaging also allows to quantify biological processes at the cellular level, to analyse both stoechiometry and dynamics of molecular interactions involved in cell and tissue regulations. Entering the new era of post-genomics requires a better knowledge of advantages and limitations of these new approaches.
authors.affiliation: Inserm U.538 et Plate-forme d'imagerie cellulaire, IFR 65, CHU Saint-Antoine, 27, rue de Chaligny, 75012 Paris, France. trugnan@st-antoine.inserm.fr
authors.forename: Germain
authors.initials: G
authors.lastname: Trugnan
authors.forename: Philippe
authors.initials: P
authors.lastname: Fontanges
authors.forename: Danièle
authors.initials: D
authors.lastname: Delautier
authors.forename: Tounsia
authors.initials: T
authors.lastname: Ait-Slimane
mesh_terms: D002468:Cell Physiological Phenomena; D023281:Genomics / Q000639:trends; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D055095:Optics and Photonics; D012984:Software