New hepatocytes for toxicology?
In a recent article M.G. Sacco and co-workers described the establishment of immortalized untransformed transgenic hepatocyte (MMH-GH) cell lines, obtained from a cross between the AT/cytoMet and Hsp70/hGH transgenic mice. This strategy proved to be successful because the MMH-GH showed stability in culture and sensitivity to chemical exposure. Based on these results, the MMH-GH cell lines could prove to be a valid alternative cell-based assay for use in toxicological studies.
Hartung
Thomas
T
ECVAM (European Centre for the Validation of Alternative Methods), IHCP (Institute for Health and Consumer Protection), JRC (Joint Research Centre), European Commission, Via E. Fermi 1, 21020 Ispra (VA), Italy.
Gribaldo
Laura
L
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000473:pathology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D014116:Toxicology / Q000379:methods*