Structural features and transcript-editing analysis of sugarcane (Saccharum officinarum L.) chloroplast genome.
The complete nucleotide sequence of the chloroplast genome of sugarcane (Saccharum officinarum) was determined. It consists of 141,182 base-pairs (bp), containing a pair of inverted repeat regions (IR(A), IR(B)) of 22,794 bp each. The IR(A) and IR(B) sequences separate a small single copy region (12,546 bp) and a large single copy (83,048 bp) region. The gene content and relative arrangement of the 116 identified genes (82 peptide-encoding genes, four ribosomal RNA genes, 30 tRNA genes), with the 16 ycf genes, are highly similar to maize. Editing events, defined as C-to-U transitions in the mRNA sequences, were comparable with those observed in maize, rice and wheat. The conservation of gene organization and mRNA editing suggests a common ancestor for the sugarcane and maize plastomes. These data provide the basis for functional analysis of plastid genes and plastid metabolism within the Poaceae. The sugarcane chloroplast DNA sequence is available at GenBank under accession NC005878.
Calsa Júnior
Tercilio
T
ESALQ/Universidade de São Paulo, Av. Pádua Dias 11, Piracicaba, 13418-900 São Paulo, Brazil.
Carraro
Dirce Maria
DM
Benatti
Matheus Romanos
MR
Barbosa
Alexandre Corrêa
AC
Kitajima
João Paulo
JP
Carrer
Helaine
H
D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D018742:DNA, Chloroplast / Q000235:genetics*; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D008969:Molecular Sequence Data; D018087:Plastids / Q000378:metabolism; D017393:RNA Editing; D018749:RNA, Plant / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D031786:Saccharum / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018742:DNA, Chloroplast; D018749:RNA, Plant