Negative ion chemical ionization-gas chromatographic-mass spectrometric determination of residues of different pyrethroid insecticides in whole blood and serum.
A new rapid and sensitive analytical method using negative ion chemical ionization-gas chromatography-mass spectrometry in selective ion monitoring mode has been developed for the determination of residues of different synthetic pyrethroid insecticides, allethrin, bifenthrin, cypermethrin, cyphonothrin, cyfluthrin, lambda-cyhalothrin, deltamethrin, fenvalerate, fenpropathrin, permethrin, prallethrin, and trans-fluthrin, in whole blood. The residues of pyrethroid molecules were extracted from the whole blood using a hexane and acetone (8:2, v/v) solvent mixture without separating the serum. The method was found sensitive to detect the residues of pyrethroids up to the level 0.2 pg/mL. Experiments conducted with the whole blood samples at the fortification level 1-100 pg/mL showed 91-103% recovery, whereas blood serum samples collected after the fortification of pyrethroids in whole blood showed 36-54% recovery. Recovery experiments conducted by direct fortification of pyrethroids in blood serum samples showed 96-108%. The applications of the analytical method was tested by analyzing 73 human blood samples collected from the population exposed continuously to different pyrethroid-based formulations. None of the blood samples showed residues of pyrethroids. The results were also confirmed by the detection of the appropriate amounts in a number of these samples, which had subsequently been spiked with known quantity of pyrethroids.
Ramesh
Atmakuru
A
Department of Analytical Chemistry, Mass Spectrometry Division, International Institute of Biotechnology and Toxicology-II BAT, Padappai, Chennai-601 301, Tamil Nadu, India. raamesh_a@hotmail.com
Ravi
Perumal Elumalai
PE
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007306:Insecticides / Q000097:blood*; D010573:Pesticide Residues / Q000097:blood*; D011722:Pyrethrins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007306:Insecticides; D010573:Pesticide Residues; D011722:Pyrethrins