title: Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye.
abstract: Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Shan
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.forename: Yanming
authors.initials: Y
authors.lastname: Jiang
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Jingxia
authors.initials: J
authors.lastname: Wen
authors.forename: You
authors.initials: Y
authors.lastname: Zhou
authors.forename: Huanwen
authors.initials: H
authors.lastname: Qian
authors.forename: Xuetao
authors.initials: X
authors.lastname: Pei
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D002478:Cells, Cultured; D004596:Electroretinography; D005260:Female; D006262:Health; D007150:Immunohistochemistry; D007211:Indoles; D007834:Lasers / Q000009:adverse effects*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology* / Q000293:injuries / Q000473:pathology* / Q000528:radiation effects
chemical_list: D007211:Indoles; C007293:DAPI