Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum.
Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1alpha at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1alpha, and that presynaptic transfection with a dominant-negative 14-3-3eta mutant, or a RIM1alpha mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1alpha phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1alpha.
Simsek-Duran
Fatma
F
Department of Pathology & Anatomy, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA.
Linden
David J
DJ
Lonart
György
G
D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology*; D051379:Mice; D009154:Mutation; D009433:Neural Inhibition / Q000235:genetics / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology*
D048948:14-3-3 Proteins