Simultaneous determination of levodopa and benserazide by stopped-flow injection analysis and three-way multivariate calibration of kinetic-spectrophotometric data.
The simultaneous determination of levodopa and benserazide in pharmaceutical formulations is described, based on the application of multidimensional partial least-squares regression to the kinetic-spectrophotometric data provided by diode-array detection within a stopped-flow injection method where analytes react with periodate. Flow injection parameters were adequately optimized. Accurate analysis is performed with no sample pre-treatment steps, and with minimum experimental effort. Satisfactory recovery results were obtained on a number of synthetic and commercial samples, in the latter case including the comparison with liquid chromatography measurements.
Pistonesi
Marcelo
M
Laboratorio FIA-Química Analítica, Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, (B8000CPB) Bahía Blanca, Argentina.
Centurión
María E
ME
Fernández Band
Beatriz S
BS
Damiani
Patricia C
PC
Olivieri
Alejandro C
AC
D001545:Benserazide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D007980:Levodopa / Q000032:analysis*; D015999:Multivariate Analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D007980:Levodopa; D001545:Benserazide