title: Arrangement of expression and distribution of tight junction protein claudin-1 in cell dissociation of pancreatic cancer cells.
abstract: Mitogen-activated protein kinase kinase 2 (MEK2) was isolated previously as a potential factor related to cancer cell dissociation in highly (PC-1.0) and weakly (PC-1) invasive pancreatic cancer cells. On the other hand, changes of structure and function of tight junction (TJ) are reported to be correlated with carcinogenesis and tumor development. In this study, immunocytochemistry and Western blot analysis were performed in pancreatic cancer cells using anti-claudin-1, MEK2 and phosphorylated MEK1/2 (p-MEK1/2) antibodies to reveal the correlation between TJ and cancer cell dissociation, as well as the involvement of MEK2 in regulation of TJ in cell dissociation of pancreatic cancer. After incubation with conditioned medium of PC-1.0 cells, plasma membrane distribution of claudin-1 was obviously disrupted, and expressions of MEK2 and p-MEK1/2, as well as dissociation of cell colonies, were significantly induced in PC-1 and CAPAN-2 cells. However, U0126 (a MEK1/2 inhibitor) treatment apparently induced the plasma membrane distribution of claudin-1 and aggregation of single cells in PC-1.0 and AsPC-1 cells, synchronously seriously suppressed MEK2 and p-MEK1/2 expression. Arrangement of expression and distribution of claudin-1 is closely related to cell dissociation status in pancreatic cancer cells through MEK2 activation.
authors.affiliation: Department of Surgery II, Kumamoto University Medical School, Kumamoto, Japan.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egami
authors.forename: Shinji
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurizaki
authors.forename: Masahide
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hirota
authors.forename: Michio
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement; D062445:Claudin-1; D006801:Humans; D007150:Immunohistochemistry; D048370:MAP Kinase Kinase 2 / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis*; D009362:Neoplasm Metastasis / Q000235:genetics* / Q000503:physiopathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D019108:Tight Junctions; D014407:Tumor Cells, Cultured
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D008565:Membrane Proteins; C482128:MAP2K2 protein, human; D048370:MAP Kinase Kinase 2