title: Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water.
abstract: Two mesophilic anaerobic bacterial strains (Z7(T) and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0.2-0.4x4.0-8.0 microm). Growth of the strains was observed at 20-42 degrees C and pH 5.0-7.5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC(15 : 0) (42.83 %) and isoC(14 : 0) (32.11 %). The DNA G+C contents of strains Z7(T) and Z1 were 50.4 and 48.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7(T) (=JCM 12359(T)=AS 1.5012(T)) as the type strain.
authors.affiliation: Graduate School of the Chinese Academy of Sciences, Beijing 100080, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Shuangya
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Dong
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004929:Esculin / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D005780:Gelatin / Q000378:metabolism; D020459:Genes, rRNA; D005840:Gentian Violet; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D002245:Carbon Dioxide; D004929:Esculin; D000431:Ethanol; D006859:Hydrogen; D005780:Gelatin; D005840:Gentian Violet; D019342:Acetic Acid