Expression microdissection: operator-independent retrieval of cells for molecular profiling.
Tissue microdissection is an important method for the study of disease states. However, it is difficult to perform high-throughput molecular analysis with current techniques. We describe here a prototype version of a novel technique (expression microdissection) that allows for the procurement of desired cells via molecular targeting. Expression microdissection (xMD) offers significant advantages over available methods, including an increase in dissection speed of several orders of magnitude. xMD may become a valuable tool for investigators studying cancer or other disease states in patient specimens and animal models.
Tangrea
Michael A
MA
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.
Chuaqui
Rodrigo F
RF
Gillespie
John W
JW
Ahram
Mamoun
M
Gannot
Gallya
G
Wallis
Benjamin S
BS
Best
Carolyn J M
CJ
Linehan
W Marston
WM
Liotta
Lance A
LA
Pohida
Thomas J
TJ
Bonner
Robert F
RF
Emmert-Buck
Michael R
MR
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004210:Dissection / Q000295:instrumentation / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D008846:Micromanipulation / Q000379:methods*; D040901:Proteomics