title: Isoperoxidases show differing sensitivity to salicylic acid.
abstract: The effects of salicylic acid (SA) on the activity of total peroxidase and the patterns of isoperoxidases of cultured tobacco cells were investigated. The total peroxidase activity of tobacco cells was inhibited by 70% when the cells were treated with 5 mM SA for one week. The peroxidase activity of tobacco cells is declined by 90% in the presence of 30 mM SA. Moreover, the activity of isoperoxidases C3, A1, and A3 decreased dramatically with increasing SA concentration, while, one of the anodic isoperoxidases, A2, was somewhat resistant to SA treatment. When isoperoxidase C3 was isolated, SA inhibited the activity of purified C3 in a concentration-dependent manner. The IC50 of isoperoxidase C3 was approximately 0.45 mM. However, the inhibition of isoperoxidase C3 activity was removed by the addition of Fe2+ ion. The possible mechanism of inhibition of peroxidase by SA is discussed.
authors.affiliation: Environmental Science Laboratory, Korea Ocean Research and Development Institute, Ansan, Kyungido, Korea.
authors.forename: EonSeon
authors.initials: E
authors.lastname: Jin
authors.forename: Man
authors.initials: M
authors.lastname: Jang
authors.forename: Kunkul
authors.initials: K
authors.lastname: Ryoo
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004305:Dose-Response Relationship, Drug; D007527:Isoenzymes; D009195:Peroxidase / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology*; D014026:Nicotiana / Q000166:cytology
chemical_list: D000890:Anti-Infective Agents; D007527:Isoenzymes; D009195:Peroxidase; D020156:Salicylic Acid