title: Determination and in-process control of zolpidem synthesis by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic assay with diode-array detection has been developed for the in-process control of zolpidem synthesis and for the analysis of the drug and its synthetic intermediates. The separation uses a 4.6mm i.d. reversed-phase Kromasil C(18) (150 mm) column, 5 microm particle size with a gradient elution mode of acetonitrile and 0.02 M NH(4)OAc (adjusted to pH 8.0) as the mobile phase (flow rate 1.0 mlmin(-1)). The analysis is performed in 12 min. The method is simple, rapid and highly specific.
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Química Física y Analítica, Julian Clavería, Oviedo, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Laviana
authors.forename: C
authors.initials: C
authors.lastname: Mangas
authors.forename: F
authors.initials: F
authors.lastname: Fernández-Marí
authors.forename: M
authors.initials: M
authors.lastname: Bayod
authors.forename: D
authors.initials: D
authors.lastname: Blanco
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D011725:Pyridines / Q000032:analysis* / Q000138:chemical synthesis*; D000077334:Zolpidem
chemical_list: D011725:Pyridines; D000077334:Zolpidem