title: [Biomaterial studies in cultures of human stapedial bone-like cells].
abstract: BACKGROUND
  Cell culture studies may provide information on the behavior of biomaterials in the intended implant environment. Cell cultures from such an environment could be used for the development of middle ear implants.
  
  
  METHODS
  Secondary bone-like cell cultures derived from human stapes were exposed to different materials [Al(2)O(3) ceramic, glass ceramic (Ceravital), gold and titanium]. Proliferation was studied for up to 40 days.
  
  
  RESULTS
  The proliferation of cultured stapes bone-like cells did not differ significantly between the four tested biomaterials. The well known cytotoxic effect of copper, which was used as a control, was evident.
  
  
  CONCLUSIONS
  Four biomaterials [Al(2)O(3) ceramic, glass ceramic (Ceravital), gold and titanium] have similar biocompatibility and no toxicity when tested in human stapes cell cultures. This in vitro model may be of considerable value for the further development of middle ear implants, e.g., when coated with bone morphogenetic proteins.
authors.affiliation: Universitäts-Hals-Nasen-Ohren-Klinik der Universität Duisburg-Essen. Dost@St-Augustinus.de
authors.forename: P
authors.initials: P
authors.lastname: Dost
authors.forename: M
authors.initials: M
authors.lastname: Wiemann
authors.forename: W J F
authors.initials: WJ
authors.lastname: ten Cate
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D001672:Biocompatible Materials / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000633:toxicity*; D006046:Gold / Q000633:toxicity*; D006801:Humans; D008422:Materials Testing; D013199:Stapes / Q000187:drug effects* / Q000473:pathology*; D014025:Titanium / Q000633:toxicity*
chemical_list: D001672:Biocompatible Materials; D006046:Gold; C031323:bio-vitroceramic AP40; D014025:Titanium; D000537:Aluminum Oxide