Molecular diagnosis of nasopharyngeal carcinoma: detecting LMP-1 and EBNA by nasopharyngeal swab.
OBJECTIVE
The aim of this study was to investigate the feasibility of molecular diagnosis of nasopharyngeal carcinoma (NPC) by combining nasopharyngeal swab and polymerase chain reaction (PCR) to detect Epstein-Barr virus (EBV) derived latent membrane protein-1 gene (LMP-1) and Epstein-Barr nuclear antigen gene (EBNA).


METHODS
437 adults underwent nasopharyngoscopy, possible biopsy, and nasopharyngeal swab to obtain nasopharyngeal cells for the detection of LMP-1 and EBNA by PCR.


RESULTS
By detecting LMP-1 and EBNA in the nasopharyngeal swabs, NPC could be diagnosed with a false-positive rate of 1.7% (6/354), a false-negative rate of 8.6% (6/70), a sensitivity of 91.4% (64/70), a specificity of 98.3% (348/354), positive predictive value of 91.4% (64/70), and negative predictive value of 98.3% (348/354).


CONCLUSIONS
Detecting EBV genomic LMP-1 and EBNA by nasopharyngeal swab verifies NPC with a sensitivity of 91.4% and specificity of 98.3%. The nasopharyngeal swab coupled with PCR based EBV LMP-1 and EBNA detection could serve as a good supplement to pathological diagnosis of NPC.
Hao
Sheng-Po
SP
Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital and Chang Gung University, Taipei, Taiwan, ROC. shengpo@adm.cgmh.org.tw
Tsang
Ngan-Ming
NM
Chang
Kai-Ping
KP
Ueng
Shir-Hwa
SH
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis*; D005240:Feasibility Studies; D006801:Humans; D008403:Mass Screening / Q000379:methods; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000032:analysis*
D014408:Biomarkers, Tumor; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D014763:Viral Matrix Proteins; C417029:EBV-encoded nuclear antigen 1