title: Novel beta-carotene ketolases from non-photosynthetic bacteria for canthaxanthin synthesis.
abstract: We reported previously that the Rhodococcus erythropolis strain AN12 synthesizes the monocyclic carotenoids 4-keto gamma-carotene and gamma-carotene. We also identified a novel lycopene beta-monocyclase in this strain. Here we report the identification of the rest of the carotenoid synthesis genes in AN12. Two of these showed apparent homology to putative phytoene dehydrogenases. Analysis of Rhodococcus knockout mutants suggested that one of them ( crtI) encodes a phytoene dehydrogenase, whereas the other ( crtO) encodes a beta-carotene ketolase. Expression of the beta-carotene ketolase gene in an Escherichia coli strain which accumulates beta-carotene resulted in the production of canthaxanthin. In vitro assays using a crude extract of the E. coli strain expressing the crtO gene confirmed its ketolase activity. A crtO homologue (DR0093) from Deinococcus radiodurans R1 was also shown to encode a beta-carotene ketolase, despite its sequence homology to phytoene dehydrogenases. The Rhodococcus and Deinococcus CrtO ketolases both catalyze the symmetric addition of two keto groups to beta-carotene to produce canthaxanthin. Even though this activity is similar to the CrtW-type of ketolase activity, the CrtO ketolases show no significant sequence homology to CrtW-type ketolases. The presence of six conserved regions may be a signature for the CrtO-type of beta-carotene ketolases.
authors.affiliation: Biological and Chemical Sciences and Engineering, Central Research and Development, Experimental Station, E328/B48, E. I. DuPont de Nemours Inc., Wilmington, DE 19880-0328, USA.
authors.forename: L
authors.initials: L
authors.lastname: Tao
authors.forename: Q
authors.initials: Q
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D016644:Canthaxanthin / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers; D034301:Deinococcus / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D012240:Rhodococcus / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D002338:Carotenoids; D016644:Canthaxanthin; D010105:Oxygenases; C093259:beta-carotene ketolase