Identification of a novel non-carbohydrate molecule that binds to the ribosomal A-site RNA.
We report the identification of a novel compound that binds to the Escherichia coli 16S ribosomal A-site. Binding by the compound was observed using nuclear magnetic resonance and mass spectrometry techniques. We show that the compound binds in the same position in the A-site RNA as occupied by the aminoglycoside class of antibiotics.
Maddaford
Shawn P
SP
MCR Research Inc., Toronto, Ontario, Canada, M3J 1P3. shawnm@nrxn.com
Motamed
Mina
M
Turner
Kevin B
KB
Choi
Min Soo K
MS
Ramnauth
Jailall
J
Rakhit
Suman
S
Hudgins
Robert R
RR
Fabris
Daniele
D
Johnson
Philip E
PE
D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000737:chemistry; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011759:Pyrrolidines / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
D006575:Heterocyclic Compounds, 3-Ring; D011759:Pyrrolidines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S