Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: biological significance.
The transcriptomes of mouse type A spermatogonia (Spga), pachytene spermatocytes (Spcy), and round spermatids (Sptd) were determined by sequencing the respective SAGE (Serial Analysis of Gene Expression) libraries. A total of 444,015 tags derived from one Spga, two Spcy, and one Sptd library were analyzed, and 34,619 different species of transcripts were identified, 5279 of which were novel. Results indicated the germ-cell transcriptome comprises of more than 30,000 transcripts. Virtual subtraction showed that cell-specific transcripts constitute 12-19.5% of the transcriptome. Components of the protein biosynthetic machinery are highly expressed in Spga. In Spcy transcription factors are abundantly expressed while transcripts encoding proteins involved in chromosome remodeling and testis-specific transcripts are prominent in Sptd. The databases generated by this work provide very useful resources for cellular localization of genes in silico. They are also extremely useful as sources for identification of splice variants of genes in germ cells.
Wu
Shao-Ming
SM
Laboratory of Clinical Genomics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA.
Baxendale
Vanessa
V
Chen
Yali
Y
Pang
Alan Lap-Yin
AL
Stitely
Timothy
T
Munson
Peter J
PJ
Leung
Michael Yiu-Kwong
MY
Ravindranath
Neelakanta
N
Dym
Martin
M
Rennert
Owen M
OM
Chan
Wai-Yee
WY
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000378:metabolism; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger