Serial analyses of gene expression (SAGE).
Serial analysis of gene expression (SAGE) is a molecular biology technique that was developed to measure the global gene expression levels. It has been applied successfully to characterize transcriptomes, compare the transcript levels between normal and diseased tissues, and uncover novel molecules within defined signal transduction pathways. A detailed description is presented in this chapter of the procedures involved to prepare the SAGE libraries. Protocols for automated sequencing and other standard molecular biology techniques can be found elsewhere, and thus are not included herein.
Dai
Jia Le
JL
Department of Molecular Pathology, M.D. Anderson Cancer Center, University of Texas, Houston, USA.
D001331:Automation; D001483:Base Sequence; D015870:Gene Expression; D015723:Gene Library; D016045:Human Genome Project; D006801:Humans; D007202:Indicators and Reagents; D008967:Molecular Biology / Q000379:methods*; D009369:Neoplasms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D007202:Indicators and Reagents; D012333:RNA, Messenger