title: IGF2 antisense transcript expression in porcine postnatal muscle is affected by a quantitative trait nucleotide in intron 3.
abstract: A paternally expressed quantitative trait locus (QTL) for muscle mass was mapped to the insulin-like growth factor II (IGF2) locus at the distal end of pig chromosome 2p. It was recently demonstrated that a G to A transition at position intron 3-nt 3072 is the quantitative trait nucleotide (QTN) underlying this QTL. In this study we report for the first time the existence of imprinted porcine IGF2 antisense transcripts (IGF2-AS) and demonstrate that their expression in postnatal muscle is also affected by the QTN. A coregulated expression of IGF2 and IGF2-AS RNAs in muscle and liver with decreasing transcription from fetal to adult age was demonstrated. Further, the significant difference found in expression of IGF2-AS in postnatal muscle between individuals with different QTL genotypes and the lack of significant differences in fetal muscle and liver reflect completely what has been found for IGF2 sense transcripts.
authors.affiliation: Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, SE-751 24 Uppsala, Sweden. martin.braunschweig@itz.unibe.ch
authors.forename: Martin H
authors.initials: MH
authors.lastname: Braunschweig
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Van Laere
authors.forename: Nadine
authors.initials: N
authors.lastname: Buys
authors.forename: Leif
authors.initials: L
authors.lastname: Andersson
authors.forename: Göran
authors.initials: G
authors.lastname: Andersson
mesh_terms: D000818:Animals; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D018392:Genomic Imprinting; D005838:Genotype; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D007438:Introns / Q000235:genetics; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics*; D009132:Muscles / Q000033:anatomy & histology / Q000378:metabolism; D010641:Phenotype; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*
chemical_list: D016372:RNA, Antisense; D007335:Insulin-Like Growth Factor II