title: Trypanocidal activity of a new diterpene from Casearia sylvestris var. lingua.
abstract: Bioassay-guided fractionation of the hexanic root bark extract of Casearia sylvestris var. lingua led to the isolation of a new clerodane diterpene, whose structure was elucidated as rel-(2 S,5 R,6 R,8 S,9 S,10 R,18 S,19 R)-19-acetoxy-18,19-epoxy-6-hydroxy-18-butanoyloxy-2-(2-methylbutanoyloxy)cleroda-3,13(16), 14-triene by spectroscopic means, including 1D and 2D NMR analyses. This compound showed pronounced activity on Trypanosoma cruzi, the casual agent of Chagas' disease, with minimal inhibitory concentration (MIC) at 0.59 microg/mL.
authors.affiliation: Laboratório de Farmacognosia, Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, Brazil. darvenne@unb.br
authors.forename: Laila Salmen
authors.initials: LS
authors.lastname: Espindola
authors.forename: José Rossy e
authors.initials: JR
authors.lastname: Vasconcelos Júnior
authors.forename: Mariana Laundry
authors.initials: ML
authors.lastname: de Mesquita
authors.forename: Patrick
authors.initials: P
authors.lastname: Marquié
authors.forename: José Elias
authors.initials: JE
authors.lastname: de Paula
authors.forename: Lengo
authors.initials: L
authors.lastname: Mambu
authors.forename: Jaime Martins
authors.initials: JM
authors.lastname: Santana
mesh_terms: D000818:Animals; D037821:Casearia; D014355:Chagas Disease / Q000188:drug therapy; D006801:Humans; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D014344:Trypanocidal Agents