Demonstration of sperm head shape abnormality and clastogenic potential of cypermethrin.
Adult male Swiss albino mice were administered ip. suspension solution of cypermethrin in 0.15% DMSO at the doses of 30 mg, 60 mg and 90 mg/kg b. wt. daily for 5 days. Another group of animals was injected cyclophosphamide ip. (60 mg/kg b. wt.) in similar manner which served as positive control. Effect of cypermethrin on body and testes weight and sperm head morphology was studied. Clastogenic potential of cypermethrin was studied by using modified Allium test. The cytological changes were studied in the root tip cells of Allium cepa after 3 days treatment with three different concentration of cypermethrin (0.1, 1.0 and 10.0 microg/ml). The results revealed that body weight gain was considerably reduced in higher dose groups, but the testicular weight did not change significantly in any of the cypermethrin treated groups. However, a significant elevation in the number of abnormal shape of sperm head was noticed in higher dose groups as compared to control. It was observed that the abnormality in the shape of sperm head was dose-dependent. The cytological changes in the root tip cells of Allium cepa indicated that cypermethrin is having toxic effects on the root tip cells in the form of stickiness of chromosomes and also affect the mitotic activity. This study suggest that cypermethrin may have the potential to induce adverse effects on sperm head shape morphology of mouse as well as clastogenic effects on root tip cells of Allium cepa.
Kumar
S
S
Division of Histochemistry and Reproductive Toxicology, National Institute of Occupational Health (ICMR), Meghani Nagar, Ahemdabad-380016, India. sunilnioh@yahoo.com
Gautam
A K
AK
Agarwal
K R
KR
Shah
B A
BA
Saiyad
H N
HN
D000490:Allium / Q000166:cytology; D000818:Animals; D001835:Body Weight; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D011722:Pyrethrins / Q000633:toxicity*; D013094:Spermatozoa / Q000002:abnormalities* / Q000187:drug effects*; D013737:Testis / Q000254:growth & development
D004785:Environmental Pollutants; D011722:Pyrethrins; C017160:cypermethrin