Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwinska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994.
A polyphasic taxonomic approach was applied to strains of the species Staphylococcus vitulinus and Staphylococcus pulvereri in order to clarify their taxonomic relatedness. Four reference strains, representing both species, and seven strains isolated from human clinical material were characterized by biotyping, ribotyping and SDS-PAGE analysis of whole-cell proteins, and none of the screening approaches allowed the two taxa to be distinguished. DNA-DNA hybridization experiments between four selected representative strains, including the type strains, confirmed that Staphylococcus pulvereri is a later synonym of Staphylococcus vitulinus.
Švec
Pavel
P
Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Sedláček
Ivo
I
Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic.
Engelbeen
Katrien
K
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Štětina
Vlastimil
V
Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Petráš
Petr
P
Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42, Praha 10, Czech Republic.
D000038:Abscess / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011015:Pneumonia, Aspiration / Q000382:microbiology; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D021521:Ribotyping; D013203:Staphylococcal Infections / Q000382:microbiology*; D013207:Staphylococcal Skin Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification*; D014556:Urine / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D020543:Proteome; D012329:RNA, Bacterial