Use of chemically modified PMMA microspheres for enzyme immobilization.
Modified poly(methyl methacrylate) (PMMA) microspheres, about 7microm in diameter, carrying aldehyde groups on their surfaces were synthesized and used as the support for enzyme immobilization. The immobilizing behavior as well as the properties of immobilized enzyme was studied. The amount of bound enzyme can be extended to 76.8mg g(-1) support, which is relatively much higher than other supports. The kinetic investigation derived from three typical models shows that the practical process is more complicated than the ideal condition, with one or more interactions being involved in the immobilization process. The K(m) value is actually larger and V(max) is smaller in the immobilized form than those in the free form. The increased resistance of the immobilized enzyme against the changes of temperature indicates that immobilizing enzyme onto the modified microspheres is useful for enzyme immobilization.
Li
Songjun
S
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, Sichuan 610041, PR China. lsjchem@hotmail.com
Hu
Jie
J
Liu
Bailing
B
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003198:Computer Simulation; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007700:Kinetics; D008422:Materials Testing; D008863:Microspheres; D008956:Models, Chemical; D010316:Particle Size; D010434:Pepsin A / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D013696:Temperature
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D019904:Polymethyl Methacrylate; D010434:Pepsin A