title: A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
abstract: Recently several strategies to treat ischemic diseases have been proposed but the ideal way has to be determined. We explored whether human placenta-derived mesenchymal cells (hPDMCs) can be used for this purpose because placenta is very rich in vessels. First, production of human vascular endothelial growth factor (hVEGF) from hPDMCs was examined. The amount of hVEGF secreted by hPDMCs was similar to the amount produced by HeLa cells. hVEGF was barely detected in human umbilical vein endothelial cells (hUVECs) or human peripheral blood mononuclear cells. hVEGF secreted from hPDMCs stimulated the proliferation of hUVECs, indicating its biological activity. Transplantation of hPDMCs to the ischemic limbs of NOD/Shi-scid mice significantly improved the blood flow of the affected limbs. Blood vessel formation was more prominently observed in the limbs of treated mice as compared to the control mice. Real-time RT-PCR revealed that hPDMCs produced hVEGF for at least 7 days after transplantation. Thus, transplantation of hPDMCs could potentially be a promising treatment for human ischemic diseases.
authors.affiliation: Department of Advanced Medical Science, Institute of Medical Science, University of Tokyo, Tokyo, Japan. tnishi-tky@umin.ac.jp
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nishishita
authors.forename: Kunie
authors.initials: K
authors.lastname: Ouchi
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.forename: Mariko
authors.initials: M
authors.lastname: Inoue
authors.forename: Takeshi
authors.initials: T
authors.lastname: Inazawa
authors.forename: Kenta
authors.initials: K
authors.lastname: Yoshiura
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kuwabara
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakaoka
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Watanabe
authors.forename: Koichi
authors.initials: K
authors.lastname: Igura
authors.forename: Tsuneo A
authors.initials: TA
authors.lastname: Takahashi
authors.forename: Naohide
authors.initials: N
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000254:growth & development; D017690:Cell Transplantation; D002478:Cells, Cultured; D005260:Female; D006367:HeLa Cells; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A