Shewanella profunda sp. nov., isolated from deep marine sediment of the Nankai Trough.
A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16 : 1omega7c, 15 : 0 iso, 16 : 0 and 13 : 0 iso. The G+C content of the DNA was 44.9 mol%. On the basis of 16S rRNA gene sequences, strain LT13a(T) was shown to belong to the gamma-Proteobacteria, being closely related to Shewanella putrefaciens (98 %), Shewanella oneidensis (97 %) and Shewanella baltica (96 %). Levels of DNA homology between strain LT13a(T) and S. putrefaciens, S. oneidensis and S. baltica were <20 %, indicating that strain LT13a(T) represents a novel species. Genetic evidence and phenotypic characteristics showed that isolate LT13a(T) constitutes a novel species of the genus Shewanella. Because of the deep origin of the strain, the name Shewanella profunda sp. nov. is proposed, with LT13a(T) (=DSM 15900(T)=JCM 12080(T)) as the type strain.
Toffin
Laurent
L
Marine Ecosystems Research Department, Japan Marine Science and Technology Centre, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.|UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
Bidault
Adeline
A
UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
Pignet
Patricia
P
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, Département de Valorisation des Produits, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
Tindall
Brian J
BJ
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Slobodkin
Alexander
A
Institute of Microbiology, RAS Prospekt 60 Let Oktyabrya 7/2, 117312 Moscow, Russia.
Kato
Chiaki
C
Marine Ecosystems Research Department, Japan Marine Science and Technology Centre, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Prieur
Daniel
D
UMR 6539, Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
D000693:Anaerobiosis; D001482:Base Composition; D003553:Cystine / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005290:Ferric Compounds / Q000378:metabolism; D005407:Flagella; D005650:Fumarates / Q000378:metabolism; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006874:Hydrostatic Pressure; D007564:Japan; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009930:Organic Chemicals / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D005290:Ferric Compounds; D005650:Fumarates; D009566:Nitrates; D009930:Organic Chemicals; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D003553:Cystine; C032005:fumaric acid; D010100:Oxygen