"One size fits it all": translocation of foreign antigens by Yersinia type III secretion system (TTSS) leads to concomitant CD4 and CD8 T-cell priming.
Live replicating bacteria expressing heterologous antigens are vaccine candidates that are able to induce complex immune responses. Yersinia pseudotuberculosis employs a type III secretion system for translocation of several virulence factors directly to the cytosol of eukaryotic cells. Mice orally inoculated with an attenuated recombinant Yersinia strain translocating a chimeric Yersinia outer protein E (YopE) molecule reveal high numbers of foreign antigen-specific CD4 and CD8 T cells. Thus, cytosolic display of a single hybrid protein results in concomitant CD4 and CD8 T-cell priming. This "one-size-fits-it-all"-feature of Yersinia-translocated heterologous antigens might be advantageous to mount T-cellular immune responses against complex microbes and tumors.
Rüssmann
Holger
H
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, D-80336 München, Germany. ruessmann@m3401.mpk.med.uni-muenchen.de
Panthel
Klaus
K
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007114:Immunization / Q000379:methods; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000276:immunology / Q000502:physiology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; C063239:yopE protein, Yersinia