A technique for preparing protein gradients on polymeric surfaces: effects on PC12 pheochromocytoma cells.
A technique for preparing micropatterns and gradients of proteins on polymeric substrates has been developed in this work. Peroxides were generated on the substrate surface by UV preirradiation and they initiated graft polymerization of acrylic acid (AA) onto the surface upon a second UV irradiation. Micropatterns and gradients of poly(acrylic acid) (PAA) were formed when the substrate was placed under or moved with respect to a photomask during UV preirradiation. Protein micropatterns and gradients were fabricated on the surface by covalently linking to the carboxyl groups on PAA chains. To test cell response to the protein gradient surfaces, PC12 pheochromocytoma cells were cultured on laminin-bound substrates in serum-free medium supplemented with nerve growth factor (NGF). It is found that both the attachment and neurite outgrowth behaviors of PC12 cells were dependent on the surface laminin density. However, the unreacted carboxyl groups on the polymer surface negatively affected PC12 cells. This weakened the positive influence from laminin.
Li
Bin
B
Institute of Materials Research and Engineering (IMRE), 3 Research Link, Singapore 117602, Republic of Singapore.
Ma
Yuexia
Y
Wang
Shu
S
Moran
Peter M
PM
D000180:Acrylic Resins / Q000737:chemistry* / Q000528:radiation effects; D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D010781:Photography / Q000379:methods; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D013499:Surface Properties; D014466:Ultraviolet Rays
D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D007797:Laminin; D011093:Polyethylene Terephthalates; C006903:carbopol 940