title: Molecular profiling of human prostate tissues: insights into gene expression patterns of prostate development during puberty.
abstract: Testosterone production surges during puberty and orchestrates massive growth and reorganization of the prostate gland, and this glandular architecture is maintained thereafter throughout adulthood. Benign prostatic hyperplasia (BPH) and prostate adenocarcinoma (PCA) are common diseases in adulthood that do not develop in the absence of androgens. Our objective was to gain insight into gene expression changes of the prostate gland at puberty, a crucial juncture in prostate development that is androgen dependent. Understanding the role played by androgens in normal prostate development may provide greater insight into androgen involvement in prostatic diseases. Benign prostate tissues obtained from pubertal and adult age group cadaveric organ donors were harvested and profiled using 20,000 element cDNA microarrays. Statistical analysis of the microarray data identified 375 genes that were differentially expressed in pubertal prostates relative to adult prostates including genes such as Nkx3.1, TMEPAI, TGFBR3, FASN, ANKH, TGFBR2, FAAH, S100P, HoxB13, fibronectin, and TSC2 among others. Comparisons of pubertal and BPH expression profiles revealed a subset of genes that shared the expression pattern between the two groups. In addition, we observed that several genes from this list were previously demonstrated to be regulated by androgen and hence could also be potential in vivo targets of androgen action in the pubertal human prostate. Promoter searches revealed the presence of androgen response elements in a cohort of genes including tumor necrosis factor-alpha induced adipose related protein, which was found to be induced by androgen. In summary, this is the first report that provides a comprehensive view of the molecular events that occur during puberty in the human prostate and provides a cohort of genes that could be potential in vivo targets of androgenic action during puberty.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602, USA.
authors.forename: Saravana Mohan
authors.initials: SM
authors.lastname: Dhanasekaran
authors.forename: Atreya
authors.initials: A
authors.lastname: Dash
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yu
authors.forename: Ira P
authors.initials: IP
authors.lastname: Maine
authors.forename: Bharathi
authors.initials: B
authors.lastname: Laxman
authors.forename: Scott A
authors.initials: SA
authors.lastname: Tomlins
authors.forename: Chad J
authors.initials: CJ
authors.lastname: Creighton
authors.forename: Anjana
authors.initials: A
authors.lastname: Menon
authors.forename: Mark A
authors.initials: MA
authors.lastname: Rubin
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
mesh_terms: D000293:Adolescent; D000328:Adult; D000728:Androgens / Q000502:physiology; D002102:Cadaver; D002648:Child; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D005796:Genes / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D011467:Prostate / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000235:genetics; D011627:Puberty / Q000235:genetics*; D014019:Tissue Donors; D001743:Urinary Bladder / Q000737:chemistry / Q000378:metabolism
chemical_list: D000728:Androgens; D018076:DNA, Complementary