Effect of heat exposure on uncoupling protein-3 mRNA abundance in porcine skeletal muscle.
Exposure to cold increases abundance of mRNA for uncoupling protein-3 (UCP3) in skeletal muscle, whereas the influence of exposure to heat is unknown. Thus, we conducted a study to investigate the influence of heat exposure on UCP3 mRNA abundance in porcine skeletal muscle. Three pigs aged 110 to 120 d, with an average BW of 75 kg, from each of eight litters were used. Each littermate was assigned to one of three treatment groups; one group was reared at 32 degrees C and fed ad libitum (32AL) for 4 wk, whereas the other two groups were maintained at 23 degrees C for the same period, and either pair-fed the intake of their 32AL littermates (23PF), or fed ad libitum (23AL). The RNase protection assay revealed that UCP3 mRNA abundance in longissimus dorsi and rhomboideus muscles was higher (P < 0.05) in the 32AL group than the 23PF group. The 23AL group also had significantly higher UCP3 mRNA abundance than the 23PF group in these muscles. Plasma total 3,5,3'-triiodothyronine concentration of the 32AL group was lower (P < 0.05) than that of the 23PF group, whereas mRNA abundance of thyroid hormone receptor (TR) isoforms, TRalpha1 and TRalpha2, in these muscles was not affected, suggesting that the 32AL group was in a relatively hypo-thyroid state. Because thyroid hormone up-regulates UCP3 expression, these results indicate that factors other than thyroid hormone may play a role in regulating UCP3 mRNA abundance in skeletal muscle of heat-exposed pigs.
Katsumata
M
M
Department of Animal and Grassland Research, National Agriculture Research Center for Kyushu Okinawa Region, Kumamoto 861-1192, Japan. masaya@affrc.go.jp
Matsumoto
M
M
Kawakami
S
S
Kaji
Y
Y
D000818:Animals; D005508:Food Deprivation; D005786:Gene Expression Regulation; D006358:Hot Temperature; D007473:Ion Channels / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics*; D037021:Thyroid Hormone Receptors alpha / Q000378:metabolism; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood / Q000378:metabolism; D000071247:Uncoupling Protein 3
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D037021:Thyroid Hormone Receptors alpha; D000071247:Uncoupling Protein 3; D014284:Triiodothyronine