Solubilization and desorption of PAHs in soil-aqueous system by biosurfactants produced from Pseudomonas aeruginosa P-CG3 under thermophilic condition.
Surface-active agents (surfactants) can enhance bioremediation of soils contaminated with PAHs by reducing sorption of PAHs or increasing desorption rates. The effectiveness of nonionic surfactant (Tween 80) and biosurfactants to enhance the solubilization and desorption of phenanthrene (Phe) and pyrene (Pyr) in soil-aqueous systems under thermophilic conditions was investigated using batch studies. Tween 80 and biosurfactants produced from Pseudomonas aeruginosa strain, ATCC 9027 and a strain isolated in our laboratory, P-CG3 were tested in this study. The results showed that all the surfactants could effectively enhance the solubility of both Phe and Pyr under thermophilic condition (55 degrees C), which was linearly proportional to the concentrations of surfactants at concentrations above their respective critical micelle concentration (CMC). When the surfactants' concentrations in aqueous phase were above their respective CMCs, substantial amounts of PAHs were desorbed from soil into the aqueous phase. Among the three surfactants used, the biosurfactant produced from our own isolate, P-CG3 was more effective in enhancing the solubilization and desorption of PAHs, implying that it might have the potential to be further applied in the bioremediation of PAH contaminated soils.
Cheng
K Y
KY
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR China.
Zhao
Z Y
ZY
Wong
J W C
JW
D000327:Adsorption; D001673:Biodegradation, Environmental; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D012989:Soil Pollutants / Q000302:isolation & purification*; D012995:Solubility; D013501:Surface-Active Agents; D013696:Temperature
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D013501:Surface-Active Agents