Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples.
Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.
Leuko
Stefan
S
Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, Australia.
Legat
Andrea
A
Fendrihan
Sergiu
S
Stan-Lotter
Helga
H
D001105:Archaea / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D015169:Colony Count, Microbial; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques; D009930:Organic Chemicals / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D012965:Sodium Chloride / Q000378:metabolism*; D013194:Staining and Labeling
D009930:Organic Chemicals; D011933:Reagent Kits, Diagnostic; C103389:SYTO 9; D012965:Sodium Chloride