Protein adsorption onto silica nanoparticles: conformational changes depend on the particles' curvature and the protein stability.
We have analyzed the adsorption of protein to the surfaces of silica nanoparticles with diameters of 6, 9, and 15 nm. The effects upon adsorption on variants of human carbonic anhydrase with differing conformational stabilities have been monitored using methods that give complementary information, i.e., circular dichroism (CD), nuclear magnetic resonance (NMR), analytical ultracentrifugation (AUC), and gel permeation chromatography. Human carbonic anhydrase I (HCAI), which is the most stable of the protein variants, establishes a dynamic equilibrium between bound and unbound protein following mixture with silica particles. Gel permeation and AUC experiments indicate that the residence time of HCAI is on the order of approximately 10 min and slowly increases with time, which allows us to study the effects of the interaction with the solid surface on the protein structure in more detail than would be possible for a process with faster kinetics. The effects on the protein conformation from the interaction have been characterized using CD and NMR measurements. This study shows that differences in particle curvature strongly influence the amount of the protein's secondary structure that is perturbed. Particles with a longer diameter allow formation of larger particle-protein interaction surfaces and cause larger perturbations of the protein's secondary structure upon interaction. In contrast, the effects on the tertiary structure seem to be independent of the particles' curvature.
Lundqvist
Martin
M
Molecular Biotechnology/IFM, Linköping University, SE-58183 Linköping, Sweden.
Sethson
Ingmar
I
Jonsson
Bengt-Harald
BH
D000327:Adsorption; D002850:Chromatography, Gel; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014461:Ultracentrifugation
D011506:Proteins; D012822:Silicon Dioxide