Thermosipho atlanticus sp. nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent.
A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV1140(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1.8 microm long and 0.4 microm wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degrees C (optimum 65 degrees C), pH 5.0-9.0 (optimum pH 6.0) and at sea salt concentrations of 20-60 g l(-1) (optimum 30 g l(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G+C content of the genomic DNA was 33 mol%. Phylogenetic analysis of the 16S rRNA gene sequence (GenBank accession number AJ577471) located the strain within the genus Thermosipho in the bacterial domain. On the basis of 16S rRNA gene sequence comparisons, and physiological and biochemical characteristics, the isolate represents a novel species, for which the name Thermosipho atlanticus sp. nov. is proposed. The type strain is DV1140(T) (=CIP 108053(T)=DSM 15807(T)).
Urios
Laurent
L
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
Cueff-Gauchard
Valérie
V
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
Pignet
Patricia
P
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
Postec
Anne
A
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
Fardeau
Marie-Laure
ML
Laboratoire IRD de Microbiologie des Anaérobies, UR 101, Universités de Provence et de la Méditerranée, CESB-ESIL, case 925, 163 Avenue de Luminy, 13288 Marseille, France.
Ollivier
Bernard
B
Laboratoire IRD de Microbiologie des Anaérobies, UR 101, Universités de Provence et de la Méditerranée, CESB-ESIL, case 925, 163 Avenue de Luminy, 13288 Marseille, France.
Barbier
Georges
G
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, IFREMER, Centre de Brest, BP 70, 29280 Plouzané, France.
D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D020459:Genes, rRNA; D045782:Hemiterpenes; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008873:Mid-Atlantic Region; D008969:Molecular Sequence Data; D009068:Movement; D009930:Organic Chemicals / Q000378:metabolism; D010421:Pentanoic Acids / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D045782:Hemiterpenes; D009930:Organic Chemicals; D010421:Pentanoic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; C008216:isovaleric acid; D006859:Hydrogen; D019342:Acetic Acid