title: An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.
abstract: General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
authors.affiliation: The Rotterdam Eye Hospital, Rotterdam, The Netherlands.
authors.forename: Luana
authors.initials: L
authors.lastname: Ugahary
authors.forename: Wendy
authors.initials: W
authors.lastname: van de Sande
authors.forename: Jan C
authors.initials: JC
authors.lastname: van Meurs
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D009877:Endophthalmitis / Q000175:diagnosis* / Q000382:microbiology; D004866:Equipment Contamination; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D011206:Povidone-Iodine / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry / Q000235:genetics; D014821:Vitrectomy
chemical_list: D000891:Anti-Infective Agents, Local; D004269:DNA, Bacterial; D011206:Povidone-Iodine