Chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline Andean region of Bolivia.
A moderately halophilic, aerobic, motile, Gram-negative, rod-shaped bacterium (strain LV4(T)) was isolated from saline soil around the lake Laguna Verde in the Bolivian Andes. The organism is a heterotroph, able to utilize various carbohydrates as a carbon source. It showed tryptophan deaminase, oxidase and catalase activity, but was unable to produce indole or H(2)S; nitrate was not reduced. The G+C content of the genomic DNA was 56.1 mol%. The pH range for growth was 5-10, temperature range was 0-45 degrees C and the range of NaCl concentrations was 0-25 % (w/v). On the basis of 16S rRNA gene sequence analysis, strain LV4(T) was found to be closely related to Chromohalobacter canadensis DSM 6769(T) and Pseudomonas beijerinckii DSM 7218(T); however, its DNA-DNA relatedness with these type strains was low. Strain LV4(T) resembled other Chromohalobacter species with respect to various physiological, biochemical and nutritional characteristics but also exhibited differences. Thus, a novel species, Chromohalobacter sarecensis sp. nov., is proposed, with LV4(T) (=CCUG 47987(T)=ATCC BAA-761(T)) as the type strain.
Quillaguamán
Jorge
J
Centro de Biotecnología, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia.|Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Delgado
Osvaldo
O
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Mattiasson
Bo
B
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Hatti-Kaul
Rajni
R
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
D000594:Amino Acid Oxidoreductases / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001838:Bolivia; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D005840:Gentian Violet; D044129:Halomonadaceae / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000378:metabolism; D050830:L-Amino Acid Oxidase; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010619:Phenazines; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D007211:Indoles; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; C030374:indole; D010088:Oxidoreductases; D002374:Catalase; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D005840:Gentian Violet; D006862:Hydrogen Sulfide