title: The modulation of caveolin-1 expression controls satellite cell activation during muscle repair.
abstract: We have previously shown that caveolin-1, the principal structural protein component of caveolar membrane domains, inhibits cellular proliferation and induces cell cycle arrest. We demonstrate here for the first time that caveolin-1 is expressed in satellite cells but not in mature muscle fibers. Satellite cells are quiescent myogenic precursors that, after muscle injury, become mitotically active, proliferate, and fuse together or, to existing myofibers, to form new muscle fibers. We show that down-regulation of caveolin-1 expression occurs in satellite cells/myogenic precursor cells (MPCs) during muscle regeneration and that hepatocyte growth factor, which is produced after muscle injury, down-regulates caveolin-1. We also demonstrate that down-regulation of endogenous caveolin-1 expression activates ERK and that activation of the p42/44 MAP kinase pathway is necessary to promote muscle regeneration. Finally, we show that overexpression of caveolin-1 inhibits muscle repair mechanisms both in vitro and in vivo. Taken together, these results propose caveolin-1 as a novel regulator of satellite cell functions and suggest that the following signaling pathway modulates satellite cell activation during muscle repair: injured fibers release HGF --> HGF down-regulates caveolin-1 protein expression --> down-regulation of caveolin-1 activates ERK --> activation of ERK promotes muscle repair by stimulating the proliferation and migration of MPCs toward the wounded area.
authors.affiliation: Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
authors.forename: Daniela
authors.initials: D
authors.lastname: Volonte
authors.forename: Youhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Galbiati
mesh_terms: D000818:Animals; D051242:Caveolin 1; D022461:Caveolins / Q000096:biosynthesis* / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics; D002461:Cell Line, Transformed; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D004789:Enzyme Activation / Q000235:genetics / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D032446:Myoblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D012038:Regeneration / Q000235:genetics / Q000502:physiology; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism* / Q000502:physiology*; D014945:Wound Healing / Q000235:genetics
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D022461:Caveolins; D017228:Hepatocyte Growth Factor; D048049:Extracellular Signal-Regulated MAP Kinases