Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga.
Two whitish yellow, Gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga Fucus evanescens. The bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) NaCl. The DNA G+C content was 61 mol%. The two isolates exhibited a conspecific DNA-DNA relatedness value of 98 %, indicating that they belong to the same species. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 3637(T) formed a distinct phyletic lineage in the genus Brevibacterium (family Brevibacteriaceae, class Actinobacteria) and showed the highest sequence similarity (about 97 %) to Brevibacterium casei. DNA-DNA hybridization experiments demonstrated 45 % binding with the DNA of B. casei DSM 20657(T). Physiological and chemotaxonomic characteristics (meso-diaminopimelic acid in the peptidoglycan, major cellular fatty acids 15 : 0ai and 17 : 0ai) of the bacteria studied were consistent with the genomic and phylogenetic data. On the basis of the results of this study, a novel species, Brevibacterium celere sp. nov., is proposed. The type strain is KMM 3637(T) (=DSM 15453(T)=ATCC BAA-809(T)).
Ivanova
Elena P
EP
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Russia.|Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
Christen
Richard
R
UMR6543 CNRS - Université de Nice Sophia Antipolis, Centre de Biochimie, Parc Valrose, F06108 Nice cedex 2, France.
Alexeeva
Yulia V
YV
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
Zhukova
Natalia V
NV
Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041, Vladivostok, Russia.
Gorshkova
Natalia M
NM
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Russia.
Lysenko
Anatoly M
AM
Institute of Microbiology of the Russian Academy of Sciences, 117811 Moscow, Russia.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Russia.
Nicolau
Dan V
DV
Industrial Research Institute, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D003960:Diaminopimelic Acid / Q000032:analysis / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D044645:Fucus / Q000382:microbiology*; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D009930:Organic Chemicals / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000302:isolation & purification; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D009930:Organic Chemicals; D010457:Peptidoglycan; D010619:Phenazines; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D003960:Diaminopimelic Acid; D005840:Gentian Violet