Optimization of medium by orthogonal matrix method for submerged mycelial culture and exopolysaccharide production in Collybia maculata.
Optimization of submerged culture conditions for the production of mycelial growth and exopolysaccharides (EPSs) by Collybia maculata was investigated. The optimum temperature and the initial pH for EPS production in a shake-flask culture of C. maculata were found to be 20 degrees C and 5.5, respectively. Among the various medium's constituents examined, glucose, Martone A-1, K(2)HPO(4), and CaCl(2) were the most suitable carbon, nitrogen, and mineral sources for EPS production, respectively. The optimum concentration of the medium's ingredients determined using the orthogonal matrix method was as follows: 30 g/L of glucose, 20 g/L of Martone A-1, 1 g/L of K(2)HPO(4), and 1 g/L of CaCl(2). Under the optimized culture conditions, the maximum concentration of EPSs in a 5-L stirred-tank reactor was 2.4 g/L, which was approximately five times higher than that in the basal medium. A comparative fermentation result showed that the EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor despite the lower mycelial growth rate. The specific productivities and the yield coefficients in the airlift reactor were higher than those in the stirred-tank reactor even though the volumetric productivities were higher in the stirred-tank reactor than in the airlift reactor.
Lim
Jong Min
JM
Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Korea.
Kim
Sang Woo
SW
Hwang
Hye Jin
HJ
Joo
Ji Hoon
JH
Kim
Hyun Oh
HO
Choi
Jang Won
JW
Yun
Jong Won
JW
D000465:Algorithms; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D005163:Factor Analysis, Statistical; D005656:Fungal Proteins / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D013696:Temperature
D003470:Culture Media; D005656:Fungal Proteins; D011134:Polysaccharides; D002244:Carbon