title: [Study on optimizing the extraction process of total free organic acids in Pinellia ternate Breit].
abstract: OBJECTIVE
  To optimize the extraction process of total free organic acids in Pinellia ternate Breit.
  
  
  METHODS
  The optimum extraction was investigated by total free organic acids determined by direct potential titration. The concentration of ethanol, amount of ethanol, extraction time and extraction times were the four factors in the experiment.
  
  
  RESULTS
  The optimum extraction process was adding 10 times amount of 75% ethanol, refluxing for 2 times, 1 h each time.
  
  
  CONCLUSIONS
  This extraction process shows higher yield of total free organic acids in Pinellia ternate Breit and is available for industrial production.
authors.affiliation: Research Center for Marine Drugs, Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Fengqing
authors.initials: F
authors.lastname: Xu
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000143:Acids / Q000032:analysis / Q000302:isolation & purification*; D000704:Analysis of Variance; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D000431:Ethanol; D010169:Palmitic Acids / Q000032:analysis / Q000302:isolation & purification; D030021:Pinellia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000032:analysis; D013386:Succinates / Q000032:analysis / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods*; D013995:Time
chemical_list: D000143:Acids; D004365:Drugs, Chinese Herbal; D010169:Palmitic Acids; D013386:Succinates; D000431:Ethanol; D012972:Sodium Hydroxide