title: Microarrays of tagged combinatorial triazine libraries in the discovery of small-molecule ligands of human IgG.
abstract: A novel and highly diverse tagged triazine library incorporating a triethylene glycol-based linker was synthesized using an orthogonal combinatorial approach on the solid phase and covalently immobilized on a glass substrate as a small molecule microarray (SMM). The SMM was screened with a fluorophore-conjugated human IgG, and 4 novel binders from a library of 2688 compounds were identified from the fully spatially addressable array without the need for compound decoding. Using surface plasmon resonance (SPR) analysis, binding seen on the array was confirmed, and a binding constant as low as Kd = 2.02 x10(-6) M was measured.
authors.affiliation: Department of Biological Sciences and Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Walsh
authors.forename: Sonya M
authors.initials: SM
authors.lastname: Khersonsky
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D020650:Combinatorial Chemistry Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D040081:Protein Array Analysis / Q000379:methods*; D013329:Structure-Activity Relationship; D014227:Triazines / Q000138:chemical synthesis*
chemical_list: D007074:Immunoglobulin G; D008024:Ligands; D014227:Triazines