Polarization-resolved second-harmonic-generation optical coherence tomography in collagen.
We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We demonstrate this new technique on a skin sample from the belly of Icelandic salmon, acquiring polarization-resolved SHOCT and OCT images simultaneously.
Applegate
Brian E
BE
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. brian.applegate@duke.edu
Yang
Changhuei
C
Rollins
Andrew M
AM
Izatt
Joseph A
JA
D000818:Animals; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods*; D012474:Salmon; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure*; D013382:Subtraction Technique; D014018:Tissue Distribution; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
D003094:Collagen