Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes.
Previous studies have demonstrated that thiamine (vitamin B1) has a cytoprotective effect against ischemic damage to the heart, and that heat shock protein 70 (Hsp70) is capable of protecting cardiac cells from lethal ischemia/hypoxia. We show here that thiamine has a cytoprotective effect on cultured neonatal rat cardiomyocytes under hypoxic insult, and also protects the cardiomyocytes against hypoxia-induced apoptosis; caspase-3 activation, PARP cleavage and DNA fragmentation are all inhibited. Moreover, it increases the level of Hsp70 protein in the cardiomyocytes even under prolonged hypoxic stress and its effects on hypoxia-induced cardiac cell death are antagonized by an Hsp70 inhibitor. These results suggest that the cytoprotective effect of thiamine in cardiomyocytes under hypoxic stress is due to its ability to induce Hsp70.
Shin
Bo Hee
BH
Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
Choi
Seung Hyuk
SH
Cho
Eun Young
EY
Shin
Min-Jeong
MJ
Hwang
Ki-Chul
KC
Cho
Hong Keun
HK
Chung
Ji Hyung
JH
Jang
Yangsoo
Y
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D053938:DNA Fragmentation; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D000860:Hypoxia / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013831:Thiamine / Q000494:pharmacology*
D018840:HSP70 Heat-Shock Proteins; D020011:Protective Agents; D011065:Poly(ADP-ribose) Polymerases; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D013831:Thiamine