Free radical scavengers in susceptible/resistant Biomphalaria alexandrina snails before and after infection.
The activities of catalase (Cat), superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione transferase (GST), glucose-6-phosphate dehydrogenase (G6PD) and glyceraldehyde3-phosphate dehydrogenase (G3PD) were studied in tissue and hemolymph of susceptible (S) (EgBS(2)) and resistant (R) (EgBR(2)) Biomphalaria alexandrina snails. The results showed that CAT and GST were higher in the hemolymph of snails susceptible to Schistosoma mansoni than in that of snails resistant to infestation, while SOD and G3PD were lower in the susceptible snails. The role of these enzymes as free radical scavengers was traced 1 and 24 h after infection of the two snail lines with S. mansoni. Moreover, the activities of SOD and G3PD were also measured 2 and 4 weeks post infection. The results revealed that the overall enzymatic activities were higher in susceptible than in resistant snail tissues. After 1 h of infection, all enzymes were increased in R and S snails except GST and G6PD which decreased in S snails. After 24 h of infection, GST increased in S snails and G3PD decreased in both S and R snails while other enzymes reached normal levels.
Mahmoud
A H
AH
Department of Medicinal Chemistry, National Research Center, Dokki, Cairo 12311, Egypt. ahlamhosny@yahoo.com
Rizk
M Z
MZ
D000818:Animals; D001700:Biomphalaria / Q000201:enzymology* / Q000276:immunology / Q000469:parasitology; D004198:Disease Susceptibility / Q000201:enzymology* / Q000276:immunology; D004199:Disease Vectors; D004798:Enzymes / Q000378:metabolism*; D016166:Free Radical Scavengers; D006790:Host-Parasite Interactions; D007113:Immunity, Innate / Q000276:immunology*; D012550:Schistosoma mansoni / Q000502:physiology*
D004798:Enzymes; D016166:Free Radical Scavengers