A common nonsense mutation in the repetitive Kringle IV-2 domain of human apolipoprotein(a) results in a truncated protein and low plasma Lp(a).
LPA, the gene coding for apolipoprotein(a) [apo(a)], is the major determinant of lipoprotein(a) [Lp(a)] plasma levels, which are associated with risk for coronary heart disease (CHD) and stroke. It is not completely understood how variation in LPA relates to Lp(a) concentrations. One type of variation related to Lp(a) levels is the number of Kringle (K) IV-2 (g.61C>T; GenBank L14005.1) repeats in LPA, but sequence variation may also contribute. Human apo(a) contains from two to >40 nearly identical K IV-2 repeats of genomic size 5.5 kb, which makes it difficult to detect mutations. To elucidate the genetic variation of the apo(a) K IV-2 domain, we isolated a single "nonexpressing" apo(a) allele with 26 K IV-2 repeats, followed by PCR, cloning and sequencing of 96 clones, resulting in an average coverage of each K IV-2 repeat of approximately four-fold. The previously described K IV types 2A and 2B (K IV-2A and K IV-2B) were detected in 74% of the clones. In addition, a new type designated 2C (K IV-2C) was present. A nonsense mutation in the first exon of K IV-2 (g.61C>T) predicted to result in a truncated protein (p.R21X) was found in nine clones on a K IV-2A background. The presence of this mutation was confirmed by analysis of genomic DNA and was shown to represent the rare allele (frequency 0.02) of a SNP. Immunoblot analysis of apo(a) from plasma confirmed the presence of a truncated apo(a) isoform in the index individual and family members. Our data show that SNPs affecting Lp(a) plasma concentrations also exist in the apo(a) K IV-2 domain.
Parson
Walther
W
Institute of Forensic Medicine, Medical University of Innsbruck, Innsbruck, Austria.
Kraft
Hans Georg
HG
Niederstätter
Harald
H
Lingenhel
Arnulf W
AW
Köchl
Silvano
S
Fresser
Friedrich
F
Utermann
Gerd
G
D001054:Apolipoproteins A / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D005060:Europe; D005260:Female; D005787:Gene Frequency; D016678:Genome; D006801:Humans; D015151:Immunoblotting; D018082:Kringles / Q000235:genetics*; D017270:Lipoprotein(a) / Q000097:blood*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid
D001054:Apolipoproteins A; D017270:Lipoprotein(a)