Differential responses of stress proteins, antioxidant enzymes, and photosynthetic efficiency to physiological stresses in the Florida red tide dinoflagellate, Karenia brevis.
This study identifies stress proteins and antioxidant enzymes that may play a role in the survival strategies of the Florida red tide dinoflagellate, Karenia brevis. Heat shock protein 60 (Hsp 60), mitochondrial small heat shock protein (mitosHsp), chloroplastic small heat shock protein (chlsHsp), Mn superoxide dismutase (SOD), and Fe SOD were first identified by Western blotting. The induction of these proteins in laboratory cultures in response to elevated temperatures, hydrogen peroxide, lead, or elevated light intensities was next assessed. In parallel, F(V)/F(M), a measurement of photosynthetic efficiency and common proxy of cellular stress, was determined. Hsp 60, Fe SOD, and Mn SOD were induced following exposure to elevated temperatures, hydrogen peroxide, or lead. MitosHsp responded only to heat, whereas chlsHsp responded only to H(2)O(2)-induced stress. The expression of stress proteins and antioxidant enzymes appears to be a more sensitive indicator of heat or chemically induced stresses than F(V)/F(M). However, F(V)/F(M) decreased significantly in response to elevated light intensities that did not induce the expression of stress proteins. These results identify for the first time stress proteins and antioxidant enzymes in K. brevis, provide evidence for differential sensitivity of cellular organelles to various sources of stress, and confirm the presence of conserved stress responses observed across phyla in a dinoflagellate.
Miller-Morey
Jeanine S
JS
Marine Biotoxins Program, Center for Coastal Environmental Health and Biomolecular Research, National Ocean Service, NOAA, 219 Fort Johnson Rd., Charleston, SC 29412, USA.
Van Dolah
Frances M
FM
D000818:Animals; D018834:Chaperonin 60 / Q000378:metabolism; D017484:Dermatitis, Phototoxic; D004141:Dinoflagellida / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007854:Lead / Q000494:pharmacology; D008027:Light; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013312:Stress, Physiological / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
D018834:Chaperonin 60; D016166:Free Radical Scavengers; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007854:Lead; C029891:lead chloride; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase