title: Evaluation of hepatosplanchnic circulation and intestinal oxygenation in dogs with a condition that mimicked septic shock induced by continuous infusion of a low dose of lipopolysaccharide.
abstract: OBJECTIVE
  To determine whether continuous infusion of a low dose of lipopolysaccharide (LPS) to induce a condition mimicking septic shock in dogs would affect systemic and hepatosplanchnic circulation and oxygenation.
  
  
  METHODS
  12 healthy adult Beagles.
  
  
  METHODS
  Dogs received a low dose of LPS (Escherichia coli O55:B5) by continuous IV infusion at a rate of 1 microg/kg/h for 8 hours. Systemic hemodynamics; systemic oxygenation; blood flow in the cranial mesenteric artery, common hepatic artery, and portal vein; intestinal and hepatic tissue blood flow; mesenteric oxygenation; and intramucosal Pco2 were examined before and at selected time points after onset of the LPS infusion.
  
  
  RESULTS
  After onset of the LPS infusion, cardiac index increased and mean arterial pressure (MAP) and systemic vascular resistance decreased, which is characteristic of the hyperdynamic state in septic patients. Hepatosplanchnic blood flow increased during the hyperdynamic state. Intestinal Pco2 was increased even when blood flows increased. During the latter half of the experimental period, MAP was maintained but hepatosplanchnic blood flows decreased and intestinal Pco2 increased further.
  
  
  CONCLUSIONS
  Analysis of the results suggested that hepatosplanchnic blood flow enters the hyperdynamic state during the early stages of sepsis and that intestinal tissue oxygenation is threatened even when hepatosplanchnic blood flow is increased or maintained. Hence, improvement of hepatosplanchnic circulation and intestinal tissue oxygenation is important in dogs with clinical evidence of a septic condition.
authors.affiliation: Division of Veterinary Surgery, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sakaue
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nezu
authors.forename: Shinobu
authors.initials: S
authors.lastname: Komori
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tagawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000378:metabolism; D004283:Dog Diseases / Q000503:physiopathology*; D004285:Dogs; D007262:Infusions, Intravenous / Q000662:veterinary; D007422:Intestines / Q000098:blood supply*; D007813:Laparotomy / Q000662:veterinary; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D010100:Oxygen / Q000097:blood; D012039:Regional Blood Flow; D012772:Shock, Septic / Q000139:chemically induced / Q000503:physiopathology / Q000662:veterinary*; D013152:Splanchnic Circulation / Q000187:drug effects*; D018709:Statistics, Nonparametric; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D002245:Carbon Dioxide; D010100:Oxygen