A role for scavenger receptor B-I in selective transfer of rhodamine-PE from liposomes to cells.
We investigated the potential role of scavenger receptor B-I (SR-BI) in the selective removal of liposomal markers from blood by hepatocytes. Liposomes were labeled with [(3)H]cholesteryloleyl-ether ([(3)H]COE), 1,2-di[1-(14)C]palmitoyl-phosphatidylcholine ([(14)C]PC), and N-(lissamine rhodamine-B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). The radiolabels were eliminated at identical rates from plasma, while N-Rh-PE was cleared twice as fast. Involvement of SR-BI in the selective removal of N-Rh-PE from liposomes was studied in transfected Chinese hamster ovary cells over-expressing SR-BI. Uptake of N-Rh-PE from liposomes containing phosphatidylserine was higher than [(3)H]COE, and was further enhanced by apolipoprotein A-I, confirming involvement of SR-BI in the selective uptake of liposomal N-Rh-PE by cells.
Yan
X
X
Department of Cell Biology, Groningen University Institute for Drug Exploration, University of Groningen, The Netherlands.
Poelstra
K
K
Scherphof
G L
GL
Kamps
J A A M
JA
D000818:Animals; D018955:CD36 Antigens; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D016503:Drug Delivery Systems / Q000379:methods; D022781:Hepatocytes / Q000378:metabolism*; D008081:Liposomes / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics*; D008297:Male; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D051116:Receptors, Scavenger; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D051122:Scavenger Receptors, Class B; D013696:Temperature; D014018:Tissue Distribution
D018955:CD36 Antigens; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger; D012235:Rhodamines; C495125:Scarb1 protein, rat; D051122:Scavenger Receptors, Class B; C046703:lissamine-rhodamine-phosphatidylethanolamine