title: One- and two-step hydrogen peroxide contact lens disinfection solutions against Acanthamoeba: how effective are they?
abstract: OBJECTIVE
  Effective contact lens disinfection solutions are important to keep the storage case free of acanthamoebae and thus prevent an infection of the eye. The aim of our study was to evaluate the effectivity of two new one-step hydrogen peroxide disinfecting solutions against Acanthamoeba spp. and compare it to the effectivity of other commercially available systems.
  
  
  METHODS
  Nine one-step 3% hydrogen peroxide systems including the new systems Silver Sept (platinum and silver disk for intensifying disinfection) and Blue Vision (newly composed catalytic tablet) and 2 two-step systems (0.6 and 3.0% H(2)O(2)) were tested for their effectivity against cysts of two Acanthamoeba keratitis isolates at different concentrations.
  
  
  RESULTS
  After a soaking time of 8 h (overnight soaking of contact lenses) the 2 two-step systems completely destroyed the cysts of both Acanthamoeba strains, even at the highest concentration of cysts tested. The nine tested one-step systems showed weaker effects. The new Blue Vision system was able to eradicate the cysts of one strain at the low concentration of cysts.
  
  
  CONCLUSIONS
  One-step hydrogen peroxide systems do not have sufficient effects on Acanthamoeba cysts and therefore may not protect the contact lens user from a possible infection of the eye. Further development of tablets like the ones used in the Blue Vision system may result in better cysticidal effects for one-step systems.
authors.affiliation: 1Department of Ophthalmology, University of Graz, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Hiti
authors.forename: J
authors.initials: J
authors.lastname: Walochnik
authors.forename: C
authors.initials: C
authors.lastname: Faschinger
authors.forename: E-M
authors.initials: EM
authors.lastname: Haller-Schober
authors.forename: H
authors.initials: H
authors.lastname: Aspöck
mesh_terms: D000048:Acanthamoeba / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D015823:Acanthamoeba Keratitis / Q000517:prevention & control; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D018639:Contact Lens Solutions / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination / Q000517:prevention & control; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects; D021261:Parasitic Sensitivity Tests
chemical_list: D000891:Anti-Infective Agents, Local; D018639:Contact Lens Solutions; D006861:Hydrogen Peroxide