title: Therapeutic basis of vidarabine on adenovirus-induced haemorrhagic cystitis.
abstract: When adenovirus causes haemorrhagic cystitis in immunocompromised patients, vidarabine is used for its treatment because therapeutic choice is limited. Although vidarabine has been reported to be effective for these patients, its therapeutic basis has not yet been established. Vidarabine dose-dependently inhibited viral replication as assessed by a yield reduction assay. Viral protein synthesis was dose-dependently inhibited by vidarabine but not at all by acyclovir, and the degree of inhibition by vidarabine was different for each of the viral proteins, ranging from 0-40% of the untreated control. These results indicated the specificity and mechanism of action of vidarabine against adenovirus. The concentration of vidarabine and its metabolite in the bladder is suggested to exhibit effective anti-adenoviral activity in suppressing the replication of adenovirus. Thus, our results support vidarabine therapy as a possible candidate for adenovirus-induced haemorrhagic cystitis in immunocompromised patients.
authors.affiliation: Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, Japan.
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Kurosaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Miwa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Kurimoto
authors.forename: Shunro
authors.initials: S
authors.lastname: Endo
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Shiraki
mesh_terms: D000212:Acyclovir / Q000008:administration & dosage / Q000627:therapeutic use; D000256:Adenoviridae / Q000187:drug effects / Q000378:metabolism; D000257:Adenoviridae Infections / Q000150:complications*; D000998:Antiviral Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003556:Cystitis / Q000188:drug therapy* / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D006470:Hemorrhage / Q000209:etiology / Q000821:virology; D006801:Humans; D016867:Immunocompromised Host / Q000187:drug effects; D001743:Urinary Bladder / Q000378:metabolism; D014740:Vidarabine / Q000627:therapeutic use*; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D014740:Vidarabine; D000212:Acyclovir