title: Amino-terminal domain of ATRIP contributes to intranuclear relocation of the ATR-ATRIP complex following DNA damage.
abstract: ATM and rad3-related protein kinase (ATR), a member of the phosphoinositide kinase-like protein kinase family, plays a critical role in cellular responses to DNA structural abnormalities in conjunction with its interacting protein, ATRIP. Here, we show that the amino-terminal portion of ATRIP is relocalized to DNA damage-induced nuclear foci in an RPA-dependent manner, despite its lack of ability to associate with ATR. In addition, ATR-free ATRIP protein can be recruited to the nuclear foci. Our results suggest that the N-terminal domain of the ATRIP protein contributes to the cell cycle checkpoint by regulating the intranuclear localization of ATR.
authors.affiliation: Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474-8522, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Itakura
authors.forename: Kaori Kajihara
authors.initials: KK
authors.lastname: Takai
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Umeda
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Mariko
authors.initials: M
authors.lastname: Ohsumi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tamai
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuura
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D064007:Ataxia Telangiectasia Mutated Proteins; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D004249:DNA Damage; D004268:DNA-Binding Proteins; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D010957:Plasmids; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000502:physiology*; D021381:Protein Transport; D034741:RNA, Small Interfering
chemical_list: C521194:ATRIP protein, human; D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; C576597:ATR protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases; D005090:Exodeoxyribonucleases; C429526:three prime repair exonuclease 1