Effect of levofloxacin on the viability of intracellular Chlamydia pneumoniae and modulation of proinflammatory cytokine production by human monocytes.
Although antibiotics are known to affect the intracellular growth of Chlamydia pneumoniae in acute infections, their efficacy in therapy for chronic infections, including atherosclerosis, remains debatable. Human monocyte-derived macrophages (MDM) obtained from monocytes of healthy donors were infected with C. pneumoniae AR-39 and treated with levofloxacin (8 microg/mL) immediately after infection (0 hours) or 24 hours after infection. Levofloxacin treatment at 24 hours, but not at 0 hours, resulted in a significant decrease in the number of C. pneumoniae inclusions within the MDM (p < 0.05). Also decreased were concentrations of proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-6, and IL-8 in the extracellular medium (p < 0.01). Viable counts in titrations remained similar to those in untreated controls. In summary, levofloxacin administered to MDM at serum-attainable levels 24 hours after C. pneumoniae infection significantly decreased inclusion counts and proinflammatory cytokine production, but did not eliminate the C. pneumoniae infection.
Baltch
Aldona L
AL
Stratton Veterans Affairs Medical Center, Albany, NY 12208, USA. aldona.baltch@med.va.gov
Smith
Raymond P
RP
Ritz
William J
WJ
Carpenter
Andrea N
AN
Bopp
Lawrence H
LH
Michelsen
Phyllis B
PB
Carlyn
Cynthia J
CJ
Hibbs
Jonathan R
JR
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000187:drug effects*; D016207:Cytokines / Q000096:biosynthesis*; D022781:Hepatocytes / Q000382:microbiology; D006801:Humans; D064704:Levofloxacin; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D015242:Ofloxacin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D016207:Cytokines; D064704:Levofloxacin; D015242:Ofloxacin