[Analysis on intraspecific variation of Cnidium monnieri by RAPD].
OBJECTIVE
To study the genetic variation of Cnidium monnieri from different regions.


METHODS
Random amplified polymophic DNA technique was used to analyze genetic polymorphy of Cnidium monnieri from 9 different regions, and dendrogram was constructed by UPGMA.


RESULTS
20 random primers were used for polymorphic selection. A total of 75 bands were amplified, 64 of which were polymorphism.


CONCLUSIONS
The smaller the geographic distances between two Cnidium monnieri, the smaller genetic differences. However, it should not be overlooked that natural environment, especially the local ecological conditions may also show some influences on the genetic variations among different C. monnieri samples.
Zhang
Qiaoyan
Q
College of Pharmacy, Second Military Medical University, Shanghai 200433.
Qin
Luping
L
Zheng
Hanchen
H
Huang
Baokang
B
Nian
Hua
H
Chen
Lei
L
D016000:Cluster Analysis; D031048:Cnidium / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005638:Fruit / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers