Loss of imprinting of PEG1/MEST in lung cancer cell lines.
Paternally expressed imprinted gene 1/mesoderm-specific transcript (PEG1/MEST) is an imprinted gene expressed from the paternal allele. Recently, frequent loss of imprinting (LOI) of PEG1/MEST has been reported in lung adenocarcinomas. It is suggested that the LOI may be involved in pathogenesis of lung adenocarcinoma. In the present study, incidence of LOI of PEG1/MEST was examined in lung cancer cell lines, including small cell lung cancer (SCLC). Among 50 cell lines tested, 20 cell lines were heterozygous for the AflIII site of the PEG1/MEST gene. In these heterozygotes, biallelic expression was observed in 9 cell lines (45%), monoallelic in 11 (55%). In cell lines of non-small cell lung cancer (NSCLC), 62% (8 of 13) exhibited biallelic expression. In SCLC, only 1 of 7 cell lines (14%) showed biallelic expression. LOI of PEG1/MEST in the NSCLC cell line is significantly frequent compared with that in SCLC cell lines (p=0.043). This result supports the possibility that LOI may be related to tumorigenesis and malignant transformation, especially in NSCLC.
Nakanishi
Hirofumi
H
Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago 683-8504, Japan.
Suda
Tetsuji
T
Katoh
Motonobu
M
Watanabe
Akiko
A
Igishi
Tadashi
T
Kodani
Masahiro
M
Matsumoto
Shingo
S
Nakamoto
Masaki
M
Shigeoka
Yasushi
Y
Okabe
Tetsuro
T
Oshimura
Mitsuo
M
Shimizu
Eiji
E
D000230:Adenocarcinoma / Q000235:genetics; D018286:Carcinoma, Giant Cell / Q000235:genetics; D018287:Carcinoma, Large Cell / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D045744:Cell Line, Tumor; D018392:Genomic Imprinting; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics*
D011506:Proteins; C103637:mesoderm specific transcript protein