[Tissue engineering of bone. Integration and migration of human mesenchymal stem cells in colonized contructs in a murine model].
Tissue engineering opens up new ways for therapy of bone defects. Therefore, the aim of this study was to establish a mouse model to investigate local cell growth of human mesenchymal stem cells (hMSC) on the scaffold in vivo. Moreover, migration of cells to other organs should be excluded.hMSC (Cambrex, USA) were cultivated according to supplier's recommendations. After inoculation on cylindric scaffolds, one matrix cell construct and one scaffold without hMSC were implanted subcutaneously left and right paravertebrally in athymic nude mice. After 2, 4, 8, and 12 weeks constructs and organs were harvested for immunohistological evaluation and PCR. In conclusion, we found integration of scaffolds loaded with hMSC implanted ectopically. HMSC seeded on 3D scaffolds survived for a period of up to 12 weeks. In addition, we could not detect hMSC in any other organ of the host.
Schieker
M
M
Chirurgische Klinik und Poliklinik-Innenstadt, Klinikum der LMU München. matthias.schieker@med.uni-muenchen.de
Seitz
S
S
Gülkan
H
H
Nentwich
M
M
Horvath
G
G
Regauer
M
M
Milz
S
S
Mutschler
W
W
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous