Impact of adiponectin gene polymorphisms on plasma lipoprotein and adiponectin concentrations of viscerally obese men.
The aim of this study was first to examine the relationships between adiponectin gene (Apm1) polymorphisms and anthropometric indices as well as plasma adiponectin and lipoprotein/lipid levels, and then to investigate whether the presence of visceral obesity or insulin resistance may modulate the impact of these polymorphisms on metabolic risk variables. Molecular screening of the Apm1 gene was achieved, and a sample of 270 unrelated men recruited from the greater Quebec City area and selected to cover a wide range of body fatness values was genotyped. Sequencing of the Apm1 gene revealed two previously reported polymorphisms (c.45T>G and c.276G>T) as well as two newly identified genetic variations (-13752delT and -13702G>C). Carriers of the c.276T allele had higher LDL-cholesterol and lower HDL-triglyceride concentrations than did 276G/G homozygotes (P=0.02 and P=0.01, respectively). Carriers of the c.45G allele exhibited higher plasma adiponectin concentrations than did 45T/T homozygotes (P=0.04). After dividing each genotype group into subgroups for visceral AT, homozygotes for the normal allele at position -13752delT, carriers of the c.45G allele, and carriers of the c.276T allele had similar total apolipoprotein B (apoB) concentrations, whether they were viscerally obese or not. These results suggest that some Apm1 gene polymorphisms influence plasma adiponectin concentrations and lipoprotein/lipid levels. In addition, the impact of these polymorphisms is modulated by the presence of visceral obesity.
Berthier
Marie-Thérèse
MT
Lipid Research Center, CHUL Research Center, Sainte-Foy, Québec, Canada.
Houde
Alain
A
Côté
Mélanie
M
Paradis
Ann-Marie
AM
Mauriège
Pascale
P
Bergeron
Jean
J
Gaudet
Daniel
D
Després
Jean-Pierre
JP
Vohl
Marie-Claude
MC
D052242:Adiponectin; D000328:Adult; D000483:Alleles; D001823:Body Composition; D015992:Body Mass Index; D001835:Body Weight; D017931:DNA Primers / Q000737:chemistry; D014644:Genetic Variation; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007328:Insulin / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood* / Q000235:genetics*; D015810:Linkage Disequilibrium; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D047495:PPAR gamma / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012306:Risk; D017422:Sequence Analysis, DNA; D013696:Temperature
D052242:Adiponectin; D017931:DNA Primers; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D008074:Lipoproteins; D047495:PPAR gamma