title: Preventive effect of fermented brown rice and rice bran on N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats.
abstract: Modifying effect of fermented brown rice by Aspergillus Oryzae (FBRA) during the initiation or post-initiation phase of N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumorigenesis was investigated in rats. Five-week-old male F344 rats were divided into 7 groups, and groups 1-5 were given subcutaneous injections of NMBA (0.5 mg/kg body weight/injection 15 times) for 5 weeks starting at 7 weeks of age. Groups 2 and 4 were fed the diet containing 5 and 10% FBRA during the initiation phase, respectively, whereas groups 3 and 5 were fed these diets during the post-initiation phase. Group 6 was given the diet containing 10% FBRA throughout the experiment, and group 7 was kept on the basal diet alone and served as an untreated control. Incidence and multiplicity of esophageal neoplasms of group 1 (NMBA alone) were 89% and 1.63+/-1.01/rat, respectively. Those of groups 3 (65%, 1.04+/-1.04) and 5 (58%, 0.77+/-0.86) were significantly less than those of group 1. Furthermore, the incidence and multiplicity of esophageal preneoplastic lesions (dysplasia) of group 5 were less than those of group 1. Post-initiation feeding of 10% FBRA significantly decreased BrdU incorporation in the non-lesional esophageal tissues when compared to that of the control. In addition, the analysis of expression levels of phase I enzymes of livers at the termination of experiment showed no clear differences among the groups. These observations indicate for the first time that FBRA inhibits NMBA-induced esophageal tumor development in rats possibly through inhibition of cell proliferation in the post-initiation phase, and suggest that FBRA is a promising dietary agent for prevention of human esophageal cancer.
authors.affiliation: Department of Tumor Pathology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan. tkuno@cc.gifu-u.ac.jp
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kuno
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hata
authors.forename: Keizou
authors.initials: K
authors.lastname: Kato
authors.forename: Sheng Hong
authors.initials: SH
authors.lastname: Qiang
authors.forename: Nami
authors.initials: N
authors.lastname: Kitaori
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
authors.forename: Teruaki
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Teruki
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Keiji
authors.initials: K
authors.lastname: Wada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet; D004128:Dimethylnitrosamine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000517:prevention & control* / Q000662:veterinary; D005285:Fermentation; D008297:Male; D009374:Neoplasms, Experimental; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; C014707:nitrosobenzylmethylamine; D004128:Dimethylnitrosamine