title: Pigmentation in intrinsically aged skin of A1 guinea pigs.
abstract: It is known that skin often shows irregular pigmentation during aging which is frequently associated with hyperpigmentation. Many studies have utilized brownish A1 guinea pigs to investigate the pathogenesis of ultraviolet (UV)-induced skin pigmentation, however, changes associated with intrinsic aging in A1 guinea pig skin have not been documented. To characterize such changes, skin from the dorsal and neck areas of 20-week, 1-, 2-, 3- and 5-yr-old guinea pigs was examined. Skin color was measured using a colorimeter, and biopsy specimens were stained with Masson-Fontana, L-3,4-dihydroxyphenylalanine (DOPA), and antibodies against KIT (ACK-45), gp100 (HMB-45) and S-100 proteins. The L* value of skin color decreased with aging and melanin deposits increased in the epidermis. Further, DOPA+, gp100+ and S-100+ melanocytes increased, indicating that the number of melanocytes had increased with age, whereas KIT+ melanocytes did not increase in dorsal skin and actually decreased in neck skin with aging. Further, rippled pigmented areas appeared in the neck skin of the 3-yr-old animals, and in the dorsal and neck skin of 5-yr-old guinea pigs in the absence of UV irradiation. Melanocytes were distributed uniformly in younger skin, whereas they were clustered in older skin. UV irradiation caused an increase in the number of melanocytes, although they were not clustered. These results are the first to provide evidence that pigmentation is induced in the skin of intrinsically aged A1 guinea pigs in the absence of UV irradiation, a process that differs from that elicited by UV irradiation.
authors.affiliation: Kanebo, Ltd Basic Research Laboratory, Odawara, Kanagawa, Japan. tobiishi@oda.cos.kanebo.co.jp
authors.forename: Megumi
authors.initials: M
authors.lastname: Tobiishi
authors.forename: Akinori
authors.initials: A
authors.lastname: Haratake
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kaminaga
authors.forename: Michio
authors.initials: M
authors.lastname: Nakahara
authors.forename: Aya
authors.initials: A
authors.lastname: Komiya
authors.forename: Hisa
authors.initials: H
authors.lastname: Koishikawa
authors.forename: Hideyo
authors.initials: H
authors.lastname: Uchiwa
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawa
authors.forename: Masako
authors.initials: M
authors.lastname: Mizoguchi
mesh_terms: D000818:Animals; D002452:Cell Count; D003124:Colorimetry / Q000379:methods; D004817:Epidermis / Q000528:radiation effects; D006168:Guinea Pigs; D008543:Melanins / Q000528:radiation effects; D008544:Melanocytes / Q000166:cytology / Q000528:radiation effects; D012867:Skin / Q000473:pathology* / Q000528:radiation effects; D015595:Skin Aging / Q000473:pathology / Q000502:physiology*; D012880:Skin Pigmentation / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins