title: Enterococcus saccharominimus sp. nov., from dairy products.
abstract: Four isolates, which were obtained from Belgian, Moroccan and Romanian dairy products, constituted a homogeneous but unidentified taxon after screening with whole-cell protein fingerprinting. Complete 16S rRNA gene sequence analysis classified representative strains in the genus Enterococcus. Highest sequence similarities of 98.6 and 98.0 % were obtained with the species Enterococcus sulfureus and Enterococcus saccharolyticus, respectively. Growth characteristics, biochemical features, tRNA intergenic length polymorphism analysis, DNA-DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel Enterococcus species. It differs phenotypically from other enterococci in characteristics commonly considered as typical of this genus: no growth in 6.5 % NaCl or 0.4 % sodium azide, and no acid production from a wide range of carbohydrates. The name Enterococcus saccharominimus sp. nov. is proposed for this novel species; the type strain (LMG 21727(T)=CCM 7220(T)) was isolated from contaminated pasteurized cow's milk.
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Institute of Biology, Romanian Academy, Cell Biology Department, Splaiul Independentei 296, 060042 Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Zamfir
authors.affiliation: Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: L A
authors.initials: LA
authors.lastname: Devriese
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Lefebvre
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Engelbeen
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Vandemeulebroecke
authors.affiliation: Laboratoire de Microbiologie et de Biologie Moléculaire, CNRST (Centre National de la Recherche Scientifique et Technique), 52 bd Omar Ibn Khatab, BP 8027-10102 Agdal, Rabat, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Amar
authors.affiliation: Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing (IMDO), Department of Applied Biological Sciences, Vrije Universiteit Brussel (VUB), Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: De Vuyst
authors.affiliation: Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Swings
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D003611:Dairy Products / Q000382:microbiology*; D016983:Enterococcus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D005516:Food Microbiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D019810:Sodium Azide / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020543:Proteome; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D012343:RNA, Transfer; D019810:Sodium Azide