title: Nocardia araoensis sp. nov. and Nocardia pneumoniae sp. nov., isolated from patients in Japan.
abstract: Two actinomycete strains isolated from two patients with lung nocardiosis between 1995 and 1997 in Japan were assigned to novel species of the genus Nocardia based on morphological and chemical criteria. Comparative 16S rRNA gene sequence analysis of the two strains revealed that they belong to the genus Nocardia and are most closely related to the species Nocardia beijingensis. Determination of DNA-DNA relatedness indicated that these strains could be assigned to two novel species. Based on their phenotypic and phylogenetic characters, two novel species of the genus Nocardia are proposed: Nocardia araoensis sp. nov. for IFM 0575(T) (=NBRC 100135(T)=JCM 12118(T)=DSM 44729(T)) and Nocardia pneumoniae sp. nov. for IFM 0784(T) (=NBRC 100136(T)=JCM 12119(T)=DSM 44730(T)).
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kageyama
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Katsukiyo
authors.initials: K
authors.lastname: Yazawa
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Mukai
authors.affiliation: Hiroshima Red Cross and Atomic Bomb Survivors Hospital, Hiroshima, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Kohara
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
mesh_terms: D000368:Aged; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D006801:Humans; D007564:Japan; D008168:Lung / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D009617:Nocardia Infections / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S