[Expression of HSP70 and P53 in malignant tumor tissues and its relationship to heat syndrome of TCM].
OBJECTIVE
To investigate the expression of HSP70 and P53 in malignant tumor tissues of patients with TCM Heat-Syndrome (HS) or with non-Heat-Syndrome (NHS).


METHODS
S-P immunohistochemical method was used to detect the gene expression, and ELISA and RT-PCR were adopted to determine the content of HSP70 and P53 mRNA expression in different malignant tumor tissues.


RESULTS
(1) Positive ratio of HSP70 and P53 in the colorectal cancer of HS was significantly higher than that in colorectal cancer of NHS respectively, and the total positive ratio of the two genes in all tumors of HS was higher than those of NHS (P<0.05); (2) HSP70 expression content in colorectal, nasopharyngeal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 expression content in tumors of HS was higher than in those of NHS (P<0.01); (3) HSP70 mRNA expression content in colorectal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 mRNA expression content in tumors of HS was higher than in those of NHS (P<0.01).


CONCLUSIONS
HSP70 and P53 positive ratio and HSP70 expression content in patients of malignant tumor with HS was higher than in those with NHS.
Wang
Hong-qi
HQ
Guangzhou University of TCM, Guangzhou 510405. jwch@gzhtcm.edu.cn
Zhang
Zheng
Z
Zhao
Yan-ping
YP
Li
Jianguo
J
Chen
Kai
K
D015179:Colorectal Neoplasms / Q000150:complications / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D005334:Fever / Q000209:etiology / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D009303:Nasopharyngeal Neoplasms / Q000150:complications / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53