title: GMP and AMP as methyl radical traps in the reaction with pentaamminemethylcobalt(III).
abstract: C8 methylation of the title purine nucleotides was achieved in near neutral aqueous solution by reaction with (13)C enriched [Co(NH(3))(5)(CH(3))](2+). This cation decomposes upon dissolution in water by release of a methyl radical. The latter was identified by electron spin resonance (ESR) spectroscopy as the PBN/(13)C(.-)H(3) adduct (PBN=phenyl-N-tert-butylnitrone). When the purine nucleotide was used in large excess, the efficiency of the trapping by the C8 atom was determined by integration of the (13)C NMR signals to be 20-25% for GMP and 15-20% for AMP, respectively, at 37 degrees C.
authors.affiliation: Department of Life Sciences and Chemistry, Roskilde University, P.O. Box 260, DK-4000 Roskilde, Denmark. pkofod@ruc.dk
authors.forename: Pauli
authors.initials: P
authors.lastname: Kofod
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry*; D005609:Free Radicals; D006157:Guanosine Monophosphate / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D009942:Organometallic Compounds; C504264:pentaamminemethylcobalt(III); D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate