title: Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C.elegans.
abstract: Ca(2+)/calmodulin-dependent calcineurin has been shown to have important roles in various Ca(2+) signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C.elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goalpha) and egl-30(Gqalpha). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C.elegans.
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
authors.forename: Jungsoo
authors.initials: J
authors.lastname: Lee
authors.forename: Changhoon
authors.initials: C
authors.lastname: Jee
authors.forename: Hyun-Ok
authors.initials: HO
authors.lastname: Song
authors.forename: Jaya
authors.initials: J
authors.lastname: Bandyopadhyay
authors.forename: Jin Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Jae-Ran
authors.initials: JR
authors.lastname: Yu
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Lee
authors.forename: Byung-Jae
authors.initials: BJ
authors.lastname: Park
authors.forename: Joohong
authors.initials: J
authors.lastname: Ahnn
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins; D019703:Calcineurin / Q000235:genetics* / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008124:Locomotion / Q000235:genetics; D008957:Models, Genetic; D010058:Oviposition / Q000235:genetics*; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000494:pharmacology; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D029742:Caenorhabditis elegans Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D012701:Serotonin; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517679:unc-43 protein, C elegans; D019703:Calcineurin; D019204:GTP-Binding Proteins