Scale-up of rifamycin B fermentation with Amycolatoposis mediterranei.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A. mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m(3) fermentor and further to a 60 m(3) fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m(3) fermentor and in the 60 m(3) fermentor reached 17.25 g/L and 19.11 g/L, respectively.
Jin
Zhi-Hua
ZH
Institute of Bioengineering, School of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China.
Lin
Jian-Ping
JP
Cen
Pei-Lin
PL
D000192:Actinomycetales / Q000254:growth & development* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D004789:Enzyme Activation; D005285:Fermentation / Q000502:physiology; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D010865:Pilot Projects; D011522:Protons; D012294:Rifamycins / Q000096:biosynthesis*; D033081:Shear Strength; D013314:Stress, Mechanical
D011522:Protons; D012294:Rifamycins; C033974:rifamycin B; D010100:Oxygen