Determination and in-process control of zolpidem synthesis by high-performance liquid chromatography.
A high-performance liquid chromatographic assay with diode-array detection has been developed for the in-process control of zolpidem synthesis and for the analysis of the drug and its synthetic intermediates. The separation uses a 4.6mm i.d. reversed-phase Kromasil C(18) (150 mm) column, 5 microm particle size with a gradient elution mode of acetonitrile and 0.02 M NH(4)OAc (adjusted to pH 8.0) as the mobile phase (flow rate 1.0 mlmin(-1)). The analysis is performed in 12 min. The method is simple, rapid and highly specific.
Laviana
L
L
Department of Physical and Analytical Chemistry, University of Oviedo, Química Física y Analítica, Julian Clavería, Oviedo, Spain.
Mangas
C
C
Fernández-Marí
F
F
Bayod
M
M
Blanco
D
D
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D011725:Pyridines / Q000032:analysis* / Q000138:chemical synthesis*; D000077334:Zolpidem
D011725:Pyridines; D000077334:Zolpidem