Effects of Bacillus subtilis O9 biosurfactant on the bioremediation of crude oil-polluted soils.
The application of a surfactant from Bacillus subtilis O9 (Bs) on the bioremediation of soils polluted with crude oil was assayed in soil microcosms under laboratory conditions. Three concentrations of biosurfactant were assayed (1.9, 19.5, and 39 mg kg(-1) soil). Microcosms without biosurfactant were prepared as controls. During the experiment, the crude oil-degrading bacterial population, the aliphatic and aromatic hydrocarbons were monitored in each microcosm. The results indicated that applying Bs did not negatively affect the hydrocarbon-degrading microbial population Concentrations of 19 and 19.5mg (Bs) per kilogram of soil stimulated the growth of the population involved in the crude oil degradation, and accelerated the biodegradation of the aliphatic hydrocarbons. However, none of the assayed Bs concentrations stimulated aromatic hydrocarbon degradation.
Cubitto
María A
MA
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000 Bahía Blanca, Argentina. mcubitto@criba.edu.ar
Morán
Ana C
AC
Commendatore
Marta
M
Chiarello
María N
MN
Baldini
Mónica D
MD
Siñeriz
Faustino
F
D001412:Bacillus subtilis / Q000737:chemistry*; D001673:Biodegradation, Environmental / Q000187:drug effects; D017753:Ecosystem; D006838:Hydrocarbons / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013501:Surface-Active Agents / Q000302:isolation & purification* / Q000494:pharmacology*
D006838:Hydrocarbons; D010578:Petroleum; D012989:Soil Pollutants; D013501:Surface-Active Agents