Upregulation of amyloid precursor protein isoforms containing Kunitz protease inhibitor in dementia with Lewy bodies.
Amyloid precursor protein (APP) is involved in the accumulation of alpha-synuclein, the main component of Lewy bodies. It is currently unknown, however, whether any of the APP isoforms is instrumental in alpha-synuclein deposition in dementia with Lewy bodies (DLB). Using real-time RT-PCR, we have studied relative mRNA expression levels of APP isoforms in frozen postmortem frontal cortices of DLB patients, Alzheimer disease (AD) patients, and control subjects. Of the three main APP isoforms, the two with a Kunitz protease inhibitory (KPI) motif (APP770 and APP751) were found to be specifically overexpressed in the frontal cortices of DLB patients when compared with controls and AD patients. These findings suggest a specific role of APP isoforms containing Kunitz protease inhibitor in DLB pathogenesis.
Beyer
Katrin
K
Department of Pathology, Hospital Universitari Germans Trias i Pujol, Autonomous University of Barcelona, Spain. jilaov@hotmail.com
Lao
José I
JI
Carrato
Cristina
C
Mate
José L
JL
López
Dolores
D
Ferrer
Isidro
I
Ariza
Aurelio
A
D000368:Aged; D000369:Aged, 80 and over; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007536:Isomerism; D020961:Lewy Body Disease / Q000378:metabolism / Q000503:physiopathology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors