[Haplotype of the BRCA2 6857delAA mutation in 4 families with breast/ovarian cancer].
OBJECTIVE
It is estimated that 5-10% of all breast cancers are hereditary, mainly are due to germline mutations in the BRCA1 and BRCA2 genes.


METHODS
A BRCA2 screening was carried out in familial breast/ovarian cancer at two centres in Spain and Chile. The 6857delAA mutation was identified in 3 Spanish families and one Chilean, all of them with Spanish ancestors. The BRCA2 haplotype of the 6857delAA carriers was analyzed using five microsatellite markers flanking the BRCA2 gene, spanning a region of 6 cM: cen-D13S260, D13S1698, (BRCA2), D13S171, D13S310 and D13S267-tel.


RESULTS
Two families shared the allelic variants of the 5 microsatellites studied. Markers D13S260 and D13S267 differed in one allele in two families, respectively. The defined haplotype was absent in non-carriers from these families, and was not detected in 100 control chromosomes without the mutation.


CONCLUSIONS
Our results suggest the existence of a common ancestry with the mutation originating in the Northeast of Spain. Given the migratory movements from Spain to Latin America, the screening of recurrent Spanish mutations can be useful in establishing a more rational and cost-effective analysis in such populations.
Campos
Berta
B
Servei de Genètica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Díez
Orland
O
Alvarez
Carolina
C
Palma
Lorena
L
Domènech
Montserrat
M
Balmaña
Judith
J
Sanz
Judit
J
Ramírez
Amaya
A
Alonso
Carmen
C
Carvallo
Pilar
P
Baiget
Montserrat
M
D001943:Breast Neoplasms / Q000235:genetics*; D002677:Chile; D005260:Female; D024522:Genes, BRCA2; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D010375:Pedigree; D013030:Spain