title: Effect of dose on house finch infection with western equine encephalomyelitis and St. Louis encephalitis viruses.
abstract: House finches, Carpodacus mexicanus, were experimentally infected with high and standard doses of western equine encephalomyelitis virus (WEEV) or St. Louis encephalitis virus (SLEV) to determine whether high doses would produce an elevated viremia response and a high frequency of chronic infections. Finches inoculated with approximately100,000 plaque forming units (PFU) of WEEV or SLEV produced viremia and antibody responses similar to those in finches inoculated with approximately 100 PFU of WEEV or 1000 PFU of SLEV, the approximate quantities of virus expectorated by blood-feeding Culex tarsalis Coquillett. Infected finches were held through winter and then necropsied. Only one finch inoculated with the high dose of SLEV developed a chronic infection. Our data indicated that elevated infectious doses of virus may not increase the viremia level or the frequency of chronic infection in house finches.
authors.affiliation: Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: William K
authors.initials: WK
authors.lastname: Reisen
authors.forename: Robert
authors.initials: R
authors.lastname: Chiles
authors.forename: Vincent
authors.initials: V
authors.lastname: Martinez
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Emily
authors.initials: E
authors.lastname: Green
authors.forename: Sharon
authors.initials: S
authors.lastname: Clark
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004665:Encephalitis Virus, St. Louis / Q000235:genetics / Q000302:isolation & purification*; D004667:Encephalitis Virus, Western Equine / Q000235:genetics / Q000302:isolation & purification*; D004674:Encephalitis, St. Louis / Q000503:physiopathology*; D020241:Encephalomyelitis, Western Equine / Q000503:physiopathology*; D046369:Finches / Q000821:virology*; D016133:Polymerase Chain Reaction; D019562:Viral Load; D014766:Viremia / Q000503:physiopathology*
chemical_list: D017931:DNA Primers