title: Genes required for Drosophila nervous system development identified by RNA interference.
abstract: RNA interference was used to screen 3,314 Drosophila double-stranded RNAs, corresponding to approximately 25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the genes are unknown. Other genes encode protein kinases, transcription factors, and signaling proteins, as well as proteins with other functions.
authors.affiliation: Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Andrej I
authors.initials: AI
authors.lastname: Ivanov
authors.forename: Alessandra C
authors.initials: AC
authors.lastname: Rovescalli
authors.forename: Paola
authors.initials: P
authors.lastname: Pozzi
authors.forename: Siuk
authors.initials: S
authors.lastname: Yoo
authors.forename: Brian
authors.initials: B
authors.lastname: Mozer
authors.forename: Hsi-Ping
authors.initials: HP
authors.lastname: Li
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Yu
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Higashida
authors.forename: Vicky
authors.initials: V
authors.lastname: Guo
authors.forename: Michael
authors.initials: M
authors.lastname: Spencer
authors.forename: Marshall
authors.initials: M
authors.lastname: Nirenberg
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D009154:Mutation; D009420:Nervous System / Q000196:embryology*; D010641:Phenotype; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded