Regulatory elements involved in transcription of the human NeuAcalpha2,3Galbeta1,3GalNAcalpha2,6-sialyltransferase (hST6GalNAc IV) gene.
We previously cloned and characterized the promoter region of the human NeuAcalpha2,3Galbeta1,3GalNAcalpha2,6-sialyltransferase (hST6GalNAc IV) gene [Kim et al. (2003)]. In the present study, we identified a region of 294 bp upstream of exon 1 of the gene that produced maximal transcriptional activity in human Jurkat T cells. Site-directed mutagenesis and transient transfection assays demonstrated that Sp1 and MZF1 elements in this region were required for the promoter activity. Further analysis by electrophoretic mobility shift assays using specific competitors and antibody revealed that Sp1 and MZF1 nuclear proteins interacted with these elements. These results indicate that Sp1 and MZF1 are involved in the transcriptional regulation of the hST6GalNAc IV gene in Jurkat T cells.
Kang
Nam-Young
NY
National Research Laboratory for Glycobiology, Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyungju 780-714, Korea.
Park
Young-Don
YD
Choi
Hee-Jung
HJ
Kim
Kyung-Sook
KS
Lee
Young-Choon
YC
Kim
Cheorl-Ho
CH
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D005809:Genes, Regulator / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D051741:Kruppel-Like Transcription Factors; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011401:Promoter Regions, Genetic; D012799:Sialyltransferases / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; C085969:MZF1 protein, human; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D012799:Sialyltransferases; C076584:(Alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl-galactosaminide 6-alpha-sialyltransferase