title: Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC.
abstract: The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. fameili@hotmail.com
authors.forename: Famei
authors.initials: F
authors.lastname: Li
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Lu
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xiong
mesh_terms: D001881:Borates; D002021:Buffers; D002316:Cardiotonic Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006835:Hydrazones / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011724:Pyridazines / Q000032:analysis* / Q000737:chemistry; D000077464:Simendan; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
chemical_list: D001881:Borates; D002021:Buffers; D002316:Cardiotonic Agents; D006835:Hydrazones; D007202:Indicators and Reagents; D011724:Pyridazines; D047392:beta-Cyclodextrins; D000077464:Simendan; C031215:betadex