title: Alternative, age- and viral load-related routes of nelfinavir resistance in human immunodeficiency virus type 1-infected children receiving highly active antiretroviral therapy.
abstract: To assess prevalence of nelfinavir resistance mutations in children receiving highly active antiretroviral therapy, sequencing of protease gene from plasma of 53 human immunodeficiency virus-infected children was performed. The prevalence of L90M was similar to that of D30N. There was a significant correlation with a higher viral load and lower age and the occurrence of L90M. These findings suggest differential molecular age- and viral load-related routes for nelfinavir resistance.
authors.affiliation: Serviço de Doenças Infecciosas e Parasitárias, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, RJ, Brazil. emachado@biologia.ufrj.br
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Machado
authors.forename: John S
authors.initials: JS
authors.lastname: Lambert
authors.forename: Adriana O
authors.initials: AO
authors.lastname: Afonso
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Cunha
authors.forename: Ricardo H
authors.initials: RH
authors.lastname: Oliveira
authors.forename: Amilcar
authors.initials: A
authors.lastname: Tanuri
authors.forename: Anne M
authors.initials: AM
authors.lastname: Sill
authors.forename: Antonio J L
authors.initials: AJ
authors.lastname: Costa
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Soares
mesh_terms: D000367:Age Factors; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000175:diagnosis / Q000188:drug therapy*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009154:Mutation; D019888:Nelfinavir / Q000494:pharmacology / Q000627:therapeutic use*; D010597:Pharmacogenetics; D012307:Risk Factors; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D019888:Nelfinavir