title: Effects of the dietary flavonoid chrysin in isolated rat mesenteric vascular bed.
abstract: In the present study, the effects of the bioflavonoid chrysin (5,7-dihydroxyflavone) were analyzed on the perfusion pressure of isolated mesenteric vascular bed. The vasorelaxant effects of chrysin were more potent on intact endothelium than on denuded vessels. This endothelium-dependent response induced by chrysin was inhibited in the presence of N(G)-nitro-L-arginine methyl ester (L-NAME), KCl, tetraethylammonium (TEA), BaCl(2), TEA plus L-NAME, and ouabain plus BaCl(2), while incubations with indomethacin and glibenclamide did not modify the response induced by this bioflavonoid. Neither gap junction inhibition with carbenoxolone nor epoxyeicosatrieconic acid synthesis inhibition with sulfaphenazole (selective CYP 2C/3A inhibitor) or 7-ethoxyresorufin (selective CYP 1A inhibitor) inhibited the chrysin-induced relaxation. Moreover, chrysin increased L-NAME-sensitive cGMP accumulation in intact vascular mesenteric preparation. In conclusion, chrysin shows vasodilator effects on resistance vessels, which depend partially on the functional endothelium and appear to be related to the NO/cGMP pathway and, possibly to the release of endothelium-derived hyperpolarizing factor.
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, ES-18071 Granada, Spain.
authors.forename: Inmaculada Concepción
authors.initials: IC
authors.lastname: Villar
authors.forename: Milagros
authors.initials: M
authors.lastname: Galisteo
authors.forename: Rocío
authors.initials: R
authors.lastname: Vera
authors.forename: Francisco
authors.initials: F
authors.lastname: O'Valle
authors.forename: María Francisca
authors.initials: MF
authors.lastname: García-Saura
authors.forename: Antonio
authors.initials: A
authors.lastname: Zarzuelo
authors.forename: Juan
authors.initials: J
authors.lastname: Duarte
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D017629:Gap Junctions / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D019255:NADPH Oxidases / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D014655:Vascular Resistance; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
chemical_list: D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D005419:Flavonoids; D026902:Potassium Channel Blockers; D017382:Reactive Oxygen Species; C043561:chrysin; D019789:Tetraethylammonium; D019255:NADPH Oxidases; D019331:NG-Nitroarginine Methyl Ester