title: Interaction between amyloid beta-protein aggregates and membranes.
abstract: The conversion of soluble, nontoxic amyloid beta-protein (Abeta) to aggregated, toxic Abeta rich in beta-sheet structures is considered to be the key step in the development of Alzheimer's disease. Therefore, extensive studies have been carried out on the mechanisms involved in Abeta aggregation and the characterization of Abeta aggregates formed in aqueous solutions mimicking biological fluids. On the other hand, several investigators pointed out that membranes play an important role in Abeta aggregation. However, it remains unclear whether Abeta aggregates formed in solution and membranes are identical and whether the former can bind to membranes. In this study, using a dye-labeled Abeta-(1-40) as well as native Abeta-(1-40), the properties of Abeta aggregates formed in buffer and raft-like membranes composed of monosialoganglioside GM1/cholesterol/sphingomyelin were compared. Fourier transform infrared spectroscopic measurements suggested that Abeta aggregates formed in buffer and in membranes have different beta-sheet structures. Fluorescence experiments revealed that Abeta aggregated in buffer did not show any affinity for membranes.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kakio
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yano
authors.forename: Denshi
authors.initials: D
authors.lastname: Takai
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsumoto
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kozutsumi
authors.forename: Katsumi
authors.initials: K
authors.lastname: Matsuzaki
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D002784:Cholesterol; D019281:Dimerization; D005677:G(M1) Ganglioside; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D013109:Sphingomyelins
chemical_list: D016229:Amyloid beta-Peptides; D008081:Liposomes; D010446:Peptide Fragments; D013109:Sphingomyelins; D005677:G(M1) Ganglioside; D002784:Cholesterol