Novel polyelectrolyte carboxymethyl konjac glucomannan-chitosan nanoparticles for drug delivery. II. Release of albumin in vitro.
Carboxymethyl konjac glucomannan-chitosan (CKGM-CS) nanoparticles were spontaneously prepared under very mild conditions via polyelectrolyte complexation. Bovine serum albumin (BSA), as a model protein drug, was incorporated into the CKGM-CS nanoparticles. The physicochemical properties of the BSA-loaded nanoparticles were identified by Zetasizer 3000 and FTIR spectrophotometry. Their sizes were from 330 nm to 900 nm; zeta potentials were positive according to varies CKGM/CS ratios. The encapsulation efficiency was up 20%. The release behavior in vitro of BSA from the nanoparticles was also investigated. We could find that the BSA release from the CKGM-CS nanoparticles is much more influenced by the CS coating layer than by the CKGM inner structure. And the CKGM-CS matrices not only exhibited pH-responsive properties, but ionic strength-sensitive properties. These systems may present a potential for pulsatile protein drug delivery.
Du
Jian
J
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, People's Republic of China.
Zhang
Sheng
S
Sun
Rui
R
Zhang
Li-Fang
LF
Xiong
Cheng-Dong
CD
Peng
Yu-Xing
YX
D003692:Delayed-Action Preparations / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods / Q000592:standards; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008351:Mannans / Q000737:chemistry* / Q000627:therapeutic use; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D009994:Osmolar Concentration; D010316:Particle Size; D011108:Polymers; D011506:Proteins / Q000008:administration & dosage*; D012709:Serum Albumin / Q000008:administration & dosage; D055672:Static Electricity
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008351:Mannans; D011108:Polymers; D011506:Proteins; D012709:Serum Albumin; C022901:(1-6)-alpha-glucomannan