title: Interaction of the fusogenic peptide B18 in its amyloid-state with lipid membranes studied by solid state NMR.
abstract: The interaction of the fusogenic polypeptide segment "B18" from the fertilization protein binding with lipid membranes was investigated by solid state 2H and 31P NMR, and by differential scanning calorimetry. B18 is known to adopt different conformations depending on peptide concentration, ionic conditions, pH and lipid environment. Here, the peptide was studied in its beta-stranded amyloid conformation. According to 31P NMR, the lamellar morphology of the DMPC bilayer remains intact in the presence of B18. In going from low (1:90) to high (1:10) peptide/lipid ratios, an increasing effect on several different 2H-labeled lipid segments was observed, reflecting changes in phase behavior and local dynamics. The strongest influence of B18 was detected at the acyl-chains, while no significant effect on the lipid headgroup conformation was observed. This suggests an insertion of B18 in its fibrillar state into the membrane driven by hydrophobic interactions, rather than a peripheral binding mediated by electrostatics.
authors.affiliation: Institute for Instrumental Analysis, Forschungszentrum Karlsruhe, P.O. Box 3640, 76021 Karlsruhe, Germany.
authors.forename: Stephan L
authors.initials: SL
authors.lastname: Grage
authors.forename: Sergii
authors.initials: S
authors.lastname: Afonin
authors.forename: Matthias
authors.initials: M
authors.lastname: Grüne
authors.forename: Anne S
authors.initials: AS
authors.lastname: Ulrich
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D001665:Binding Sites; D003903:Deuterium; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000737:chemistry; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D044367:Phase Transition; D010761:Phosphorus Radioisotopes; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D000682:Amyloid; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010455:Peptides; D010761:Phosphorus Radioisotopes; D003903:Deuterium; D004134:Dimyristoylphosphatidylcholine