title: [Visual cycle and dark adaptation: a new approach in research].
abstract: Visual cycle is the series of reactions that support regeneration of the visual pigmen after its photolysis in retinal rods and cones. Inherited or acquired deficiencies of the visual cycle impair dark adaptation and lead to a series of visual disorders. The paper describes a new approach to study of the visual cycle that uses fast dichroic microspectrophotometer. The method allows studying interconversion of bleaching products in single intact photoreceptors in condition approaching the situation in vivo. Using this approach, we established a complete scheme of transitions between metarhodopsins, retinal and retinol in amphibian rods. It appeared that the decay of metarhodopsins controls both the time course of rod dark adaptation following small bleaches and the production of retinol that is the substrate for rhodopsin regeneration. We also obtained novel data on kinetics of the decay of cone metapigments that was found to be by an order of magnitude faster than in rods. Possible application of the method for further study of the visual cycle in normal and pathological conditions is discussed.
authors.forename: V I
authors.initials: VI
authors.lastname: Govardovskiĭ
authors.forename: S A
authors.initials: SA
authors.lastname: Shukoliukov
authors.forename: A V
authors.initials: AV
authors.lastname: Kolesnikov
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Golobkova
mesh_terms: D000663:Amphibians; D000818:Animals; D003623:Dark Adaptation / Q000502:physiology*; D007700:Kinetics; D015591:Microspectrophotometry / Q000379:methods*; D038761:Photobleaching; D012172:Retinaldehyde / Q000096:biosynthesis; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000502:physiology*; D014785:Vision, Ocular / Q000502:physiology*; D014801:Vitamin A / Q000096:biosynthesis
chemical_list: D014801:Vitamin A; D012243:Rhodopsin; D012172:Retinaldehyde