Immobilization of glucoamylase onto novel porous polymer supports of vinylene carbonate and 2-hydroxyethyl methacrylate.
Glucoamylase was immobilized onto novel porous polymer supports. The properties of immobilized glucoamylase and the relationship between the activity of immobilized enzyme and the properties of porous polymer supports were investigated. Compared with the native enzyme, the temperature profile of immobilized glucoamylase was widened, and the optimum pH was also changed. The optimum substrate concentration of immobilized glucoamylase was higher than that of native enzyme. After storage for 23 d, the immobilized glucoamylase still maintained about 84% of its initial activity, whereas the native enzyme only maintained about 58% of the initial activity. Moreover, after using repeatedly seven times, the immobilized enzyme maintained about 85% of its initial activity. Furthermore, the properties of porous polymer supports had an effect on the activity of the immobilized glucoamylase.
Huo
Yanli
Y
Department of Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, P.R. China. ylhuo@fudan.edu.cn
Li
Yue
Y
Yuan
Zhi
Z
Huang
Jiaxian
J
D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D004149:Dioxoles / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D013213:Starch / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature
D001672:Biocompatible Materials; D004149:Dioxoles; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D008689:Methacrylates; D011108:Polymers; C005044:hydroxyethyl methacrylate; C031134:vinylene carbonate; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase