title: Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina.
abstract: Two novel 21-residue antimicrobial peptides, arenicin-1 and arenicin-2, exhibiting activity against Gram-positive and Gram-negative bacteria and fungi, were purified from coelomocytes of marine polychaeta Arenicola marina (lugworm) by preparative gel electrophoresis and RP-HPLC. Molecular masses (2758.3 and 2772.3 Da) and complete amino acid sequences (RWCVYAYVRVRGVLVRYRRCW and RWCVYAYVRIRGVLVRYRRCW) were determined for each isoform. Each arenicin has one disulfide bond (Cys3-Cys20). The total RNA was isolated from the lugworm coelomocytes, RT-PCR and cloning were performed, and cDNA was sequenced. A 202-residue preproarenicin contains a putative signal peptide (25 amino acids) and a long prodomain. Arenicins have no structure similarity to any previously identified antimicrobial peptides.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str. 16/10, 117997 Moscow, Russia. ovch@ibch.ru
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: Galina M
authors.initials: GM
authors.lastname: Aleshina
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Balandin
authors.forename: Anna D
authors.initials: AD
authors.lastname: Krasnosdembskaya
authors.forename: Mikhail L
authors.initials: ML
authors.lastname: Markelov
authors.forename: Elena I
authors.initials: EI
authors.lastname: Frolova
authors.forename: Yulia F
authors.initials: YF
authors.lastname: Leonova
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Tagaev
authors.forename: Eugeny G
authors.initials: EG
authors.lastname: Krasnodembsky
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Kokryakov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides; D011077:Polychaeta / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers; D015801:Helminth Proteins; D010455:Peptides; C496888:arenicin-1, Arenicola marina; D012313:RNA