title: Control of growth and survival of Listeria monocytogenes on smoked salmon by combined potassium lactate and sodium diacetate and freezing stress during refrigeration and frozen storage.
abstract: In this study, we evaluated the antimicrobial effects of different levels of a potassium lactate (PL) plus sodium diacetate (SDA) mixture against the growth and survival of Listeria monocytogenes Scott A inoculated onto smoked salmon stored at 4, 10, and -20 degrees C. The effect of freezing stress on the growth kinetics of L. monocytogenes Scott A on smoked salmon at 4 and 10 degrees C was also investigated. The use of PL+SDA at all tested levels (1.5, 3.3, and 5% of a 60% commercial solution of PURASAL P Opti. Form 4) completely inhibited the growth of L. monocytogenes Scott A on smoked salmon stored at 4 degrees C during 32 days of storage. It also delayed the growth of L. monocytogenes Scott A on smoked salmon stored at 10 degrees C for up to 11 days, but a listeriostatic effect was observed only with 5% PURASAL P Opti. Form 4 at 10 degrees C after 11 days. Addition of PL+SDA at all tested levels decreased the surviving populations of L monocytogenes Scott A on smoked salmon during 10 months of frozen storage at -20 degrees C. Freezing stress significantly (P < 0.001) extended the lag time and delayed the growth of L. monocytogenes Scott A at both 4 and 10 degrees C. However, the effect of freezing stress was more significant at 4 degrees C than at 10 degrees C, indicating the importance of temperature control of smoked salmon during the retail storage period.
authors.affiliation: Center for Food Science and Technology, University of Maryland Eastern Shore, Princess Anne, Maryland 21853, USA. ksyoon@umes.edu
authors.forename: K S
authors.initials: KS
authors.lastname: Yoon
authors.forename: C N
authors.initials: CN
authors.lastname: Burnette
authors.forename: K A
authors.initials: KA
authors.lastname: Abou-Zeid
authors.forename: R C
authors.initials: RC
authors.lastname: Whiting
mesh_terms: D000818:Animals; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005615:Freezing; D007773:Lactates / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D012034:Refrigeration; D012474:Salmon / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology*; D019346:Sodium Acetate / Q000494:pharmacology; D013997:Time Factors
chemical_list: D004202:Disinfectants; D007773:Lactates; D019346:Sodium Acetate