Plant cell cultures for the production of recombinant proteins.
The use of whole plants for the synthesis of recombinant proteins has received a great deal of attention recently because of advantages in economy, scalability and safety compared with traditional microbial and mammalian production systems. However, production systems that use whole plants lack several of the intrinsic benefits of cultured cells, including the precise control over growth conditions, batch-to-batch product consistency, a high level of containment and the ability to produce recombinant proteins in compliance with good manufacturing practice. Plant cell cultures combine the merits of whole-plant systems with those of microbial and animal cell cultures, and already have an established track record for the production of valuable therapeutic secondary metabolites. Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems.
Hellwig
Stephan
S
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Worringerweg 1, D-52074 Aachen, Germany.
Drossard
Jürgen
J
Twyman
Richard M
RM
Fischer
Rainer
R
D001688:Biological Products / Q000096:biosynthesis / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015398:Signal Transduction / Q000502:physiology
D001688:Biological Products; D010940:Plant Proteins; D011994:Recombinant Proteins