Bovine respiratory syncytial virus ISCOMs--protection in the presence of maternal antibodies.
The protection induced by immunostimulating complexes (ISCOMs) against bovine respiratory syncytial virus (BRSV) was evaluated and compared to that of a commercial inactivated vaccine (CV) in calves with BRSV-specific maternal antibodies. Following experimental challenge, controls (n = 4) and animals immunized with CV (n = 5) developed moderate to severe respiratory disease, whereas calves immunized with ISCOMs (n = 5) remained clinically healthy. BRSV was re-isolated from the nasopharynx of all controls and from all calves immunized with CV, but from none of the calves immunized with ISCOMs. BRSV-RNA was detected by real-time PCR from a single animal in this group. Significantly higher BRSV-specific nasal IgG, serum IgG1 and IgG2 titers were detected before and after challenge in animals immunized with ISCOMs versus CV. In conclusion, the ISCOMs overcame the suppressive effect of maternal antibodies in calves and induced strong clinical and virological protection against a BRSV challenge.
Hägglund
Sara
S
Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, P.O. Box 7019, SE-75007 Uppsala, Sweden. sara.hagglund@idmed.slu.se
Hu
Ke-Fei
KF
Larsen
Lars Erik
LE
Hakhverdyan
Mikhayil
M
Valarcher
Jean-François
JF
Taylor
Geraldine
G
Morein
Bror
B
Belák
Sándor
S
Alenius
Stefan
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D016882:ISCOMs / Q000008:administration & dosage* / Q000276:immunology; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D009305:Nasopharynx / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control / Q000662:veterinary*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage* / Q000276:immunology; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology* / Q000302:isolation & purification; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016882:ISCOMs; D007074:Immunoglobulin G; D012367:RNA, Viral; D022261:Respiratory Syncytial Virus Vaccines; D015164:Vaccines, Inactivated; D022223:Vaccines, Subunit