Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells.
It has previously been demonstrated that baculovirus infection of the Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines leads to oxidative stress as measured by protein and membrane lipid oxidation and that this oxidative damage contributes to cell death. As a result of these findings, it was hypothesized that baculovirus infection stimulates superoxide radical (O(2)(.-)) synthesis in the mitochondria and that the resulting O(2)(.-) accumulation overwhelms the cells' antioxidant defenses. We investigated the ability of manganese superoxide dismutase (MnSOD) expression (which reduces O(2)(.-) to H(2)O(2) to overcome the oxidative damage caused by baculovirus infection. It was found that MnSOD expression significantly reduced oxidative damage in baculovirus-infected Tn-5B1-4 cells but had no significant effect on oxidative damage in baculovirus-infected Sf-9 cells. The results are consistent with the hypothesis that O(2)(.-) accumulation in the mitochondria is at least partially responsible for the oxidative damage resulting from the baculovirus infection of insect cells.
Wang
Ying
Y
Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, IA 52242, USA.
Oberley
Larry W
LW
Howe
Dale
D
Jarvis
Donald L
DL
Chauhan
Gaurav
G
Murhammer
David W
DW
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D007915:Lepidoptera / Q000201:enzymology* / Q000821:virology*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera / Q000201:enzymology / Q000821:virology; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000379:methods*; D014777:Virus Diseases / Q000201:enzymology* / Q000821:virology*
D011994:Recombinant Proteins; D013482:Superoxide Dismutase