Primaclade--a flexible tool to find conserved PCR primers across multiple species.
Primaclade is a web-based application that accepts a multiple species nucleotide alignment file as input and identifies a set of polymerase chain reaction (PCR) primers that will bind across the alignment. Primaclade iteratively runs the Primer3 application for each alignment sequence and collates the results. Primaclade creates an HTML results page that recaps the original alignment, provides a consensus sequence and lists primers for each alignment area, with primers color-coded to reflect the level of degeneracy in the primer.
Gadberry
Michael D
MD
Department of Biology, University of Missouri-St. Louis, One University Boulevard, St. Louis, MO 63121, USA.
Malcomber
Simon T
ST
Doust
Andrew N
AN
Kellogg
Elizabeth A
EA
D000465:Algorithms; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013045:Species Specificity
D017931:DNA Primers