[Fundamental studies on locally injected methotrexate bound to activated carbon particles (MTX-CH)].
We developed a new dosage formulation of methotrexate bound to activated carbon particles (MTX-CH). In this study, subcutaneous injection of MTX-CH was examined for its long-acting effect at the injection sites, anti-tumor effect and acute toxicity in mice. MTX-CH- or MTX aqueous solution (MTX-SOL)- was injected locally into tumors growing on the back of BALB/c mice at a dosage of 30 mg/kg as methotrexate (MTX). (1) The MTX concentration at the injection sites remained higher in mice with MTX-CH than that with MTX-SOL. (2) A marked effect on the control of tumor growth by MTX-CH was noted after repeated injections throughout the observation period. These results suggest that MTX-CH is superior to MTX-SOL due to longer-acting effects at the administration site and have a better control of tumor growth than MTX aqueous solution (MTX-SOL).
Fujiyama
Junshin
J
Dept. Surgery, Gamo Hospital.
Nakase
Yuen
Y
Mochizuki
Satoshi
S
Togawa
Takeshi
T
Suzuki
Genichi
G
Hagiwara
Akeo
A
Yamagishi
Hisakazu
H
D000327:Adsorption; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage*; D002606:Charcoal / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D004304:Dosage Forms; D015552:Injections, Intralesional; D007279:Injections, Subcutaneous; D008727:Methotrexate / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy; D012996:Solutions
D000964:Antimetabolites, Antineoplastic; D003692:Delayed-Action Preparations; D004304:Dosage Forms; D012996:Solutions; D002606:Charcoal; D008727:Methotrexate