Direct observation of anion-mediated translocation of fluorescent oligoarginine carriers into and across bulk liquid and anionic bilayer membranes.
The recent hypothesis that counteranion-mediated dynamic inversion of charge and solubility might contribute to diverse functions of oligoarginines in biomembranes was tested with two fluorescently labelled oligomers, FL-R(8), one of the most active cell-penetrating peptides, and its longer version, FL-R(16). We report evidence for counteranion-mediated phase transfer from water into bulk chloroform and anionic lipid-bilayer membranes as well as reverse-phase transfer from bulk chloroform and across intact lipid-bilayer membranes into water. The differences found between FL-R(8) and FL-R(16) with regard to location in the bilayer and reverse-phase transfer from bulk and lipid-bilayer membranes into water implied that the reported results may be relevant for biological function.
Sakai
Naomi
N
Department of Organic Chemistry, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland. naomi.sakai@chiorg.unige.ch
Takeuchi
Toshihide
T
Futaki
Shiroh
S
Matile
Stefan
S
D017920:Antiporters / Q000737:chemistry* / Q000502:physiology; D001120:Arginine / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry; D021381:Protein Transport; D012995:Solubility
D017920:Antiporters; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D009842:Oligopeptides; D001120:Arginine