title: Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes.
abstract: Previous studies have demonstrated that thiamine (vitamin B1) has a cytoprotective effect against ischemic damage to the heart, and that heat shock protein 70 (Hsp70) is capable of protecting cardiac cells from lethal ischemia/hypoxia. We show here that thiamine has a cytoprotective effect on cultured neonatal rat cardiomyocytes under hypoxic insult, and also protects the cardiomyocytes against hypoxia-induced apoptosis; caspase-3 activation, PARP cleavage and DNA fragmentation are all inhibited. Moreover, it increases the level of Hsp70 protein in the cardiomyocytes even under prolonged hypoxic stress and its effects on hypoxia-induced cardiac cell death are antagonized by an Hsp70 inhibitor. These results suggest that the cytoprotective effect of thiamine in cardiomyocytes under hypoxic stress is due to its ability to induce Hsp70.
authors.affiliation: Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Bo Hee
authors.initials: BH
authors.lastname: Shin
authors.forename: Seung Hyuk
authors.initials: SH
authors.lastname: Choi
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Cho
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Shin
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Hwang
authors.forename: Hong Keun
authors.initials: HK
authors.lastname: Cho
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Chung
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Jang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D053938:DNA Fragmentation; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology; D000860:Hypoxia / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013831:Thiamine / Q000494:pharmacology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D020011:Protective Agents; D011065:Poly(ADP-ribose) Polymerases; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D013831:Thiamine