Proteome analysis of human substantia nigra in Parkinson's disease.
Protein expression has been compared in human substantia nigra specimens from Parkinson's disease (PD) patients and from controls, and 44 proteins expressed in this midbrain region were identified by peptide mass fingerprinting. Among them, nine showed changes in their abundance. L and M neurofilament chains are less abundant in PD specimens, whereas peroxiredoxin II, mitochondrial complex III, ATP synthase D chain, complexin I, profilin, L-type calcium channel delta-subunit, and fatty-acid binding protein are significantly more present in PD samples than in controls. Besides the consolidated view of oxidative stress involvement in PD pathogenesis, suggested by overexpression of mitochondrial and reactive oxygen species (ROS)-scavenging proteins, these results indicate a possible potentiation mechanism of afferent signals to substantia nigra following degeneration of dopaminergic neurons.
Basso
Manuela
M
Department of Structural and Functional Biology, and Center of Neuroscience, University of Insubria, Busto Arsizio, Italy.
Giraudo
Sabrina
S
Corpillo
Davide
D
Bergamasco
Bruno
B
Lopiano
Leonardo
L
Fasano
Mauro
M
D000368:Aged; D001921:Brain / Q000378:metabolism; D019992:Databases as Topic; D004298:Dopamine / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress; D010300:Parkinson Disease / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013378:Substantia Nigra / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D011506:Proteins; D017382:Reactive Oxygen Species; D014357:Trypsin; D004298:Dopamine