title: Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene.
abstract: OBJECTIVE
  To test an experimental model of chemical mammary carcinogenesis induction in rats.
  
  
  METHODS
  Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy.
  
  
  RESULTS
  Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis.
  
  
  CONCLUSIONS
  This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.
authors.affiliation: Discipline of Gynecology, Hospital das Clínicas, Faculty of Medicine, University of São Paulo - São Paulo/SP, Brazil.
authors.forename: Alfredo Carlos S D
authors.initials: AC
authors.lastname: Barros
authors.forename: Elisa Naomi K
authors.initials: EN
authors.lastname: Muranaka
authors.forename: Lincon Jo
authors.initials: LJ
authors.lastname: Mori
authors.forename: Christina Helena T
authors.initials: CH
authors.lastname: Pelizon
authors.forename: Kyoshi
authors.initials: K
authors.lastname: Iriya
authors.forename: Giovana
authors.initials: G
authors.lastname: Giocondo
authors.forename: José Aristodemo
authors.initials: JA
authors.lastname: Pinotti
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene