title: Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo.
abstract: One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Lay-Pheng
authors.initials: LP
authors.lastname: Tan
authors.forename: Rina Y P
authors.initials: RY
authors.lastname: Lue
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D019921:Biotinylation; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D047668:Inteins / Q000502:physiology; D040081:Protein Array Analysis / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D049452:Green Fluorescent Proteins; D001710:Biotin