title: Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum: insights into the puzzling difference between unready and ready oxidized inactive states.
abstract: Dynamic electrochemical studies, incorporating catalytic voltammetry and detailed potential-step manipulations, provide compelling evidence that the oxidized inactive state of [NiFe]-hydrogenases termed Unready (or Ni-A) contains a product of partial reduction of O(2) that is trapped in the active site.
authors.affiliation: Department of Chemistry, Inorganic Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QR, England.
authors.forename: Sophie E
authors.initials: SE
authors.lastname: Lamle
authors.forename: Simon P J
authors.initials: SP
authors.lastname: Albracht
authors.forename: Fraser A
authors.initials: FA
authors.lastname: Armstrong
mesh_terms: D000332:Aerobiosis; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002841:Chromatiaceae / Q000201:enzymology*; D004563:Electrochemistry; D004789:Enzyme Activation; D006864:Hydrogenase / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; C043296:nickel-iron hydrogenase; D006864:Hydrogenase; D010100:Oxygen