title: alpha3beta1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development.
abstract: We show that alpha3 integrin mutation disrupts distinct aspects of neuronal migration and placement in the cerebral cortex. The preplate develops normally in alpha3 integrin mutant mice. However, time lapse imaging of migrating neurons in embryonic cortical slices indicates retarded radial and tangential migration of neurons, but not ventricular zone-directed migration. Examination of the actin cytoskeleton of alpha3 integrin mutant cortical cells reveals aberrant actin cytoskeletal dynamics at the leading edges. Deficits are also evident in the ability of developing neurons to probe their cellular environment with filopodial and lamellipodial activity. Calbindin or calretinin positive upper layer neurons as well as the deep layer neurons of alpha3 integrin mutant mice expressing EGFP were misplaced. These results suggest that alpha3beta1 integrin deficiency impairs distinct patterns of neuronal migration and placement through dysregulated actin dynamics and defective ability to search and respond to migration modulating cues in the developing cortex.
authors.affiliation: UNC Neuroscience Center and the Department of Cell and Molecular Physiology, The University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Ralf S
authors.initials: RS
authors.lastname: Schmid
authors.forename: Stephanie
authors.initials: S
authors.lastname: Shelton
authors.forename: Amelia
authors.initials: A
authors.lastname: Stanco
authors.forename: Yukako
authors.initials: Y
authors.lastname: Yokota
authors.forename: Jordan A
authors.initials: JA
authors.lastname: Kreidberg
authors.forename: E S
authors.initials: ES
authors.lastname: Anton
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000196:embryology* / Q000378:metabolism; D039201:Integrin alpha3beta1 / Q000378:metabolism*; D051379:Mice; D008818:Mice, Neurologic Mutants; D009474:Neurons / Q000166:cytology* / Q000378:metabolism
chemical_list: D000199:Actins; D039201:Integrin alpha3beta1