Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance.
Dehydration responsive element binding protein 1 (DREB1)/C-repeat binding factor (CBF) induces the expression of many stress-inducible genes in Arabidopsis. We have previously reported the identification of three DREB1/ICBF homologs from sweet cherry (Prunus avium). To identify the function of these homologs, one of the genes, CIG-B, was transformed into Arabidopsis. In one of the transgenic plant lines, the DREB1/CBF target gene cor15a was induced in the absence of stress treatment. The cor15a-overexpressing transgenic plant exhibited mild growth retardation and had greater salt and freezing tolerance than did the wild-type and the transgenic lines in which cor15a was not induced. These results suggest that this sweet cherry DREB1/CBF homolog has a function similar to that of DREB1/CBF.
Kitashiba
Hiroyasu
H
JST, Yamagata Regional Joint Research Project, 423 Shimaminami, Sagae, Yamagata 991-0043, Japan. kite@affrc.go.jp
Ishizaka
Takako
T
Isuzugawa
Kanji
K
Nishimura
Kouichi
K
Suzuki
Takashi
T
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D010641:Phenotype; D010940:Plant Proteins / Q000096:biosynthesis / Q000502:physiology*; D030821:Plants, Genetically Modified; D027861:Prunus / Q000737:chemistry*; D012965:Sodium Chloride / Q000633:toxicity*; D014157:Transcription Factors / Q000096:biosynthesis / Q000502:physiology*
D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride