Type II isopentenyl diphosphate isomerase from Synechocystis sp. strain PCC 6803.
Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni(2+) chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg(2+) for activity; K(m)(IPP) was 52 microM, and k(cat)(IPP) was 0.23 s(-1).
Barkley
Sam J
SJ
Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Desai
Shrivallabh B
SB
Poulter
C Dale
CD
D019748:Carbon-Carbon Double Bond Isomerases / Q000302:isolation & purification* / Q000378:metabolism; D045782:Hemiterpenes; D007700:Kinetics; D046939:Synechocystis / Q000201:enzymology*
D045782:Hemiterpenes; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase