High-performance liquid chromatographic method for simultaneous determination of baicalein and baicalein 7-glucuronide in rat plasma.
A simple HPLC method with ultraviolet detection was developed for the simultaneous determination of baicalein and its major metabolite, baicalein 7-glucuronide (baicalin) in rat plasma. Following solid phase extraction with HLB cartridge, the analytes were separated using a gradient mobile phase consisting of methanol-acetonitrile-phosphate buffer. The flow-rate was set at 1ml/min and the eluent was detected at 320nm. The method is linear over the studied range of 1-10 and 0.05-1microg/ml for baicalein and baicalin, respectively. The intra-day and inter-day variations of the analysis were less than 6.38%, with relative error ranging from -3.6 to 6%. The limit of quantification in plasma was 0.05microg/ml for both baicalein and baicalin. The developed method proves to be an improved and more reliable method because of its capability for determining baicalein and baicalin simultaneously in a single chromatographic run.
Zhang
Li
L
School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, PRC.
Lin
Ge
G
Zuo
Zhong
Z
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044950:Flavanones / Q000097:blood* / Q000737:chemistry; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein