title: Solid state protein monolayers: morphological, conformational, and functional properties.
abstract: We have studied the morphological, conformational, and electron-transfer (ET) function of the metalloprotein azurin in the solid state, by a combination of physical investigation methods, namely atomic force microscopy, intrinsic fluorescence spectroscopy, and scanning tunneling microscopy. We demonstrate that a "solid state protein film" maintains its nativelike conformation and ET function, even after removal of the aqueous solvent.
authors.affiliation: National Nanotechnology Laboratories of INFM, Biomolecular Electronics Division, Department of Innovation Engineering, University of Lecce, Via per Arnesano, 73100 Lecce, Italy.
authors.forename: P P
authors.initials: PP
authors.lastname: Pompa
authors.forename: A
authors.initials: A
authors.lastname: Biasco
authors.forename: V
authors.initials: V
authors.lastname: Frascerra
authors.forename: F
authors.initials: F
authors.lastname: Calabi
authors.forename: R
authors.initials: R
authors.lastname: Cingolani
authors.forename: R
authors.initials: R
authors.lastname: Rinaldi
authors.forename: M Ph
authors.initials: MP
authors.lastname: Verbeet
authors.forename: E
authors.initials: E
authors.lastname: de Waal
authors.forename: G W
authors.initials: GW
authors.lastname: Canters
mesh_terms: D000327:Adsorption; D001400:Azurin / Q000737:chemistry* / Q000648:ultrastructure*; D004579:Electron Transport; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D011485:Protein Binding; D011487:Protein Conformation; D012997:Solvents / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry*
chemical_list: D046912:Multiprotein Complexes; D012997:Solvents; D014867:Water; D001400:Azurin