Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.
We report here on the identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. Immunocytochemical experiments confirm that the enzyme indeed localizes to mitochondrial compartment. Inhibition of expression of the enzyme by RNA interference results in significant shortening of the poly(A) tails of the mitochondrial ND3, COX III and ATP 6/8 transcripts, suggesting that the investigated protein represents a bona fide mitochondrial poly(A) polymerase. This is in agreement with our sequencing data which show that poly(A) tails of several mitochondrial messengers are composed almost exclusively of adenosine residues. Moreover, the data presented here indicate that all analyzed mitochondrial transcripts with profoundly shortened poly(A) tails are relatively stable, which in turn argues against the direct role of long poly(A) extensions in the stabilization of human mitochondrial messengers.
Tomecki
Rafal
R
Department of Genetics, Warsaw University, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
Dmochowska
Aleksandra
A
Gewartowski
Kamil
K
Dziembowski
Andrzej
A
Stepien
Piotr P
PP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002467:Cell Nucleus / Q000235:genetics; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D026721:RNA 3' End Processing; D034622:RNA Interference; D020871:RNA Stability; D000077278:RNA, Mitochondrial; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
D000077278:RNA, Mitochondrial; D012313:RNA; D004259:DNA-Directed DNA Polymerase