Response to acute changes in salinity of two different muscle type creatine kinase isoforms, from euryhaline teleost (Oreochromis mossambicus) gills.
Two CKM isoforms (CKM1 and CKM2) from the gills of tilapia (Oreochromis mossambicus) were obtained after transfer from freshwater (FW) to seawater (SW, 25 ppt). Based on the 5' and 3' RACE, the identity of CKM1 and CKM2 was determined to be 59% in the 5'-untranslated region (5'-UTR) and 41.9% in the 3'-UTR. Using Northern blot hybridization with the CKM1 and CKM2 3'-UTR probes, CKM1 and CKM2 were found to be expressed in muscle, heart and gill. The levels of these two different CK isoforms (CKM1 and CKM2) were shown to be different in FW than after acute SW transfer, showing that CKM isoforms respond to changes in salinity.
Gong
Hong-Yi
HY
Institute of Zoology, Academia Sinica, Nankang, Taiwan.
Wu
Jen-Leih
JL
Huang
Wei-Tung
WT
Ji-Fan Lin
Cliff
C
Weng
Ching-Feng
CF
D000064:Acclimatization; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D005618:Fresh Water; D005880:Gills / Q000201:enzymology; D006321:Heart / Q000502:physiology; D009132:Muscles / Q000201:enzymology*; D020033:Protein Isoforms; D012623:Seawater / Q000009:adverse effects; D012965:Sodium Chloride / Q000494:pharmacology*; D017210:Tilapia / Q000378:metabolism*
D020033:Protein Isoforms; D012965:Sodium Chloride; D003402:Creatine Kinase