The malic enzyme is required for optimal photoautotrophic growth of Synechocystis sp. strain PCC 6803 under continuous light but not under a diurnal light regimen.
A mutation was recovered in the slr0721 gene, which encodes the decarboxylating NADP(+)-dependent malic enzyme in the cyanobacterium Synechocystis sp. strain PCC 6803, yielding the mutant 3WEZ. Under continuous light, 3WEZ exhibits poor photoautotrophic growth while growing photoheterotrophically on glucose at rates nearly indistinguishable from wild-type rates. Interestingly, under diurnal light conditions (12 h of light and 12 h of dark), normal photoautotrophic growth of the mutant is completely restored.
Bricker
Terry M
TM
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA. btbric@lsu.edu
Zhang
Shulu
S
Laborde
Susan M
SM
Mayer
Paul R
PR
Frankel
Laurie K
LK
Moroney
James V
JV
D003624:Darkness; D008027:Light; D008291:Malate Dehydrogenase / Q000502:physiology*; D011770:Pyruvate Kinase / Q000502:physiology; D046939:Synechocystis / Q000201:enzymology / Q000254:growth & development*
D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D011770:Pyruvate Kinase