title: P-cadherin is up-regulated by the antiestrogen ICI 182,780 and promotes invasion of human breast cancer cells.
abstract: P-cadherin expression in breast carcinomas has been associated with tumors of high histologic grade and lacking estrogen receptor-alpha, suggesting a link between these proteins. In the MCF-7/AZ breast cancer cell line, blocking estrogen receptor-alpha signaling with the antiestrogen ICI 182,780 induced an increase of P-cadherin, which coincided with induction of in vitro invasion. Retroviral transduction of MCF-7/AZ cells, as well as HEK 293T cells, showed the proinvasive activity of P-cadherin, which requires the juxtamembrane domain of its cytoplasmic tail. This study establishes a direct link between P-cadherin expression and the lack of estrogen receptor-alpha signaling in breast cancer cells and suggests a role for P-cadherin in invasion, through its interaction with proteins bound to the juxtamembrane domain.
authors.affiliation: Institute of Pathology and Molecular Immunology of Porto University, Porto, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Paredes
authors.forename: Christophe
authors.initials: C
authors.lastname: Stove
authors.forename: Veronique
authors.initials: V
authors.lastname: Stove
authors.forename: Fernanda
authors.initials: F
authors.lastname: Milanezi
authors.forename: Veerle
authors.initials: V
authors.lastname: Van Marck
authors.forename: Lara
authors.initials: L
authors.lastname: Derycke
authors.forename: Marc
authors.initials: M
authors.lastname: Mareel
authors.forename: Marc
authors.initials: M
authors.lastname: Bracke
authors.forename: Fernando
authors.initials: F
authors.lastname: Schmitt
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D017931:DNA Primers; D018076:DNA, Complementary; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D020847:Estrogen Receptor Modulators / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D000077267:Fulvestrant; D006801:Humans; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D015820:Cadherins; D017931:DNA Primers; D018076:DNA, Complementary; D020847:Estrogen Receptor Modulators; D047628:Estrogen Receptor alpha; D000077267:Fulvestrant; D004958:Estradiol