Human serum albumin: spectroscopic studies of the paclitaxel binding and proximity relationships with cisplatin and adriamycin.
The interactions of anti-cancer drugs with blood constituents, particularly with serum albumin (HSA) may have a major influence on drug pharmacology and efficacy. In the present work the binding of paclitaxel (trade name Taxol) to human serum albumin and its effect on cisplatin and adriamycin interactions has been investigated through UV/visible, CD, fluorescence spectroscopy and the inductively couplet plasma atomic emission spectroscopy method. Displacement studies with use of bilirubin, as a competitive agent provided relevant information about the location of the binding site in HSA as well as the possible multidrug interactions.
Trynda-Lemiesz
Lilianna
L
Faculty of Chemistry, University of Wrocław, F.Joliot-Curie 14, 50383 Wrocław, Poland. Ltl@wchuwr.chemi.uni.wroc.pl
Luczkowski
Marek
M
D000970:Antineoplastic Agents / Q000097:blood / Q000737:chemistry; D001663:Bilirubin / Q000737:chemistry; D001665:Binding Sites; D002942:Circular Dichroism; D002945:Cisplatin / Q000097:blood / Q000737:chemistry; D004317:Doxorubicin / Q000097:blood* / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D017239:Paclitaxel / Q000097:blood* / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*
D000970:Antineoplastic Agents; D012709:Serum Albumin; D004317:Doxorubicin; D017239:Paclitaxel; D002945:Cisplatin; D001663:Bilirubin