title: A short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames.
abstract: N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 nt). Compared with YSMV-RNA 3, N20-RNA 3L contains an additional 97 nt in the 5' leader upstream of the open reading frames for movement protein (MP) and coat protein (CP). Two overlapping unidentified reading frames (URF1 and URF2) result from this modification, each of which code for putative translation products of 21 amino acids. The URF1 putative peptide has a hydrophilic N-terminus and a hydrophobic C-terminus, indicating a possible association with both host cell membrane and cytosol whereas the putative URF2 product is predominantly hydrophobic. A further structural modification found in N20-RNA 3L is a new tandem repeat of 243 nts which overlaps with the MP open reading frame.
authors.affiliation: Agriculture Western Australia, 444 Albany Highway, Western Australia, Australia. kjayasena@agric.wa.gov.au
authors.forename: Kithsiri W
authors.initials: KW
authors.lastname: Jayasena
authors.forename: John W
authors.initials: JW
authors.lastname: Randles
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D017794:Alfalfa mosaic virus / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D023061:Gene Order; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D020080:Tandem Repeat Sequences / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins