Evans Blue is an inhibitor of nuclear factor-kappa B (NF-kappaB)-DNA binding.
Nuclear factor-kappa B (NF-kappaB) is an important transcription factor, involved in many immune and inflammatory responses. It is critical in HIV gene expression as it has kappa B binding sites in the HIV-1 long-terminal repeat. Hence, targeting NF-kappaB to prevent its DNA binding holds a significant therapeutic potential. In this context, we report Evans Blue as a novel inhibitor of NF-kappaB-DNA binding. Evans Blue was found to be inhibiting DNA binding of NF-kappaB at a low concentration of 100 microM. Further, molecular modeling studies using docking and generation of electrostatic potential maps predicted a possible binding mode of EB to the DNA binding region of NF-kappaB, consistent with the experimental activity.
Sharma
Rakesh K
RK
Department of Chemistry, Delhi University, Delhi-7, India.
Otsuka
Masami
M
Pande
Vineet
V
Inoue
Jun-ichiro
J
João Ramos
Maria
M
D002467:Cell Nucleus / Q000737:chemistry*; D004247:DNA / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005070:Evans Blue / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D013329:Structure-Activity Relationship
D016328:NF-kappa B; D005070:Evans Blue; D004247:DNA