Differential expression of protein kinase C alpha and delta in testes of mouse at various stages of development.
Protein kinase C (PKC) describes a family of serine/threonine protein kinases, and multiple isoforms are expressed in various mammalian tissues. In the present study, we examined the expression of PKC-alpha and PKC-delta at protein and mRNA level in mouse testis by Western blotting and RT-PCR. We also examined the expression of both PKC isoenzymes in the developing mouse testis. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC-alpha were uniformly distributed; but PKC-delta expression occurred in the testes of 3-week-old mice, perhaps even at a relatively late stage in spermatid development. The results suggest that each isoenzyme may have different functional roles in processing and modulating physiological cellular responses of spermatogenesis.
Feng
Chen
C
Department of Biochemistry, China Medical University, Shenyang, Liaoning Province, PRC.
Zhang
Jie
J
Gasana
Vénuste
V
Fu
Wei
W
Liu
Yi
Y
Zong
ZhiHong
Z
Yu
BingZhi
B
D000375:Aging / Q000502:physiology; D000818:Animals; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism*; D051745:Protein Kinase C-delta / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development*
D007527:Isoenzymes; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta