title: Genotyping accuracy for whole-genome amplification of DNA from buccal epithelial cells.
abstract: We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/phi29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.
authors.affiliation: Department of Genetics, University of North Carolina at Chapel Hill, 27599-7264, USA.
authors.forename: Keren
authors.initials: K
authors.lastname: Leviel
authors.forename: Megan
authors.initials: M
authors.lastname: Olarte
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Sullivan
mesh_terms: D004247:DNA / Q000032:analysis*; D004847:Epithelial Cells; D005784:Gene Amplification / Q000235:genetics; D016678:Genome; D005838:Genotype; D006801:Humans; D008214:Lymphocytes; D009061:Mouth Mucosa
chemical_list: D004247:DNA