Non-induced leukocyte extract reduces HIV replication and TNF secretion.
According to UNAIDS, the global HIV/AIDS epidemic increased to 40 million the number of people living with the virus around the world. Dialyzable leukocyte extract obtained by our group is a low molecular weight dialyzable material from peripheral human leukocytes previously in vitro induced with Sendai virus (DLE-ind), and more recently, from non-induced leukocytes (DLE n/i). Previous results have shown the ability of DLE-ind to inhibit HIV in vitro replication in MT4 cell; to reduce TNFalpha secretion, and to delay in vivo progression to AIDS in early stage of HIV infection. In this work we present evidences that DLE n/i also inhibits HIV in vitro replication and reduces TNFalpha secretion in human whole blood like DLE obtained from induced leukocytes. Taking together these results show that both properties of DLE, HIV in vitro inhibition and TNF production modulation, are not dependent on in vitro Sendai virus induction of leukocytes.
Fernández-Ortega
Celia
C
Department of Cell Biology, Center for Genetic Engineering and Biotechnology, Ave. 31 e/ 158 y 190, Cubanacán, Playa, AP: 6162, Havana 10600, Cuba. celia.fernandez@cigb.edu.cu
Dubed
Marta
M
Ramos
Yudith
Y
Navea
Leonor
L
Alvarez
Giselle
G
Lobaina
Leonor
L
López
Lissette
L
Casillas
Dionne
D
Rodríguez
Licet
L
D001773:Blood Cells / Q000187:drug effects / Q000821:virology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D013601:T-Lymphocytes / Q000187:drug effects / Q000821:virology*; D014165:Transfer Factor / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
D008070:Lipopolysaccharides; D014165:Transfer Factor; D014409:Tumor Necrosis Factor-alpha; C440499:lipopolysaccharide, Escherichia coli O111 B4