ATFC is a novel transducer for the unfolded protein response in Bombyx mori BM5 cells.
We report the isolation of activating transcription factor of chaperone (ATFC), a novel cDNA from Bombyx mori BM5 cells that encodes a putative transducer of endoplasmic reticulum (ER) stress. The 236 amino acids of ATFC include both a basic region and a leucine zipper at the C-terminus, in contrast to Hac1p of yeast which features these structures at its N-terminus. ATFC expression was strongly up-regulated by ER stress. ATFC could specifically bind to the unfolded protein response element. BM5 cells transfected with ATFC cDNA displayed enhanced ER chaperone expression in response to ER stress. These results indicate that ATFC encodes a putative transducer of ER stress.
Goo
Tae Won
TW
Department of Sericulture and Entomology, The National Institute of Agriculture, Science, and Technology, R.D.A., Suwon 441-100, Republic of Korea.
Yun
Eun Young
EY
Choi
Kwang Ho
KH
Kim
Sung-Hwan
SH
Nho
Si-Kab
SK
Kang
Seok-Woo
SW
Kwon
O-Yu
OY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000166:cytology; D002460:Cell Line; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics; D018832:Molecular Chaperones / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D015003:Yeasts
D018076:DNA, Complementary; D005656:Fungal Proteins; D018832:Molecular Chaperones; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014415:Tunicamycin