title: Somatostatin down-regulates LFA-1 activation by modulating Rap1 expression in CD4+ and CD8+ T cells.
abstract: Leukocyte function-associated antigen-1 (LFA-1) is one of the integrins that are expressed on the leukocytes, and has been shown to play an important role in leukocyte trafficking. The adhesive activity of LFA-1 is governed partially by the Rap1. This study examined that the relationship between LFA-1 and Rap1 mRNA expressions by anti-CD3 and anti-CD3+SOM treatment in the CD4+ and CD8+ T cells. The LFA-1 mRNA expression levels following the anti-CD3 and anti-CD3+SOM treatment for 30 min was greater on the CD8+ T cells, and the LFA-1 expression of the CD8+ T cells with anti-CD+SOM treatment was affected more severely than that of the CD4+ T cells. The Rap1 mRNA expression patterns following anti-CD3 and anti-CD3+SOM stimulation in the CD4+ and CD8+ T cells were similar to the LFA-1 expression patterns, and the expression level following anti-CD3+SOM treatment was suppressed more significantly in the CD8+ T cells. These results suggest that the difference in the Rap1 expression level after stimulation might explain the differences in the LFA-1 expression level on the T cell subsets, and that the down-regulation of Rap1 expression following SOM treatment is closely related to the diminished LFA-1 expression.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, South Korea.
authors.forename: Won-Kee
authors.initials: WK
authors.lastname: Yoon
authors.forename: Ho-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Son
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
authors.forename: Sang-Joonn
authors.initials: SJ
authors.lastname: Park
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: An
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Se-Ra
authors.initials: SR
authors.lastname: Kim
authors.forename: Si-Yun
authors.initials: SY
authors.lastname: Ryu
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism; D020827:rap1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D013004:Somatostatin; D020827:rap1 GTP-Binding Proteins