Identification of target proteins of N-acetylglucosaminyl-transferase V and fucosyltransferase 8 in human gastric tissues by glycomic approach.
To understand better the mechanism underlying gastric cancer and search for potential markers for gastric cancer prognosis, the proteomic analysis of gastric cancer tissues was conducted using two-dimensional gel electrophoresis and lectin blot, followed by electrospray ionization-mass spectrometry. These approaches permitted identification of glyco- or putative glycosylated proteins which may be associated with tumorigenesis. The proteins identified include molecules involved in sugar metabolism, signal transduction, proteolysis, and stress, as well as several unknown proteins, which were aberrantly glycosylated as evidenced by the L-phytohemagglutinin blot.
Kim
Yong-Sam
YS
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
Hwang
Soo Young
SY
Oh
Sejeong
S
Sohn
Hosung
H
Kang
Hye-Yeon
HY
Lee
Jeong-Hwa
JH
Cho
Eun-Wie
EW
Kim
Jin-Young
JY
Yoo
Jong Shin
JS
Kim
Nam-Soon
NS
Kim
Cheorl-Ho
CH
Miyoshi
Eiji
E
Taniguchi
Naoyuki
N
Ko
Jeong Heon
JH
D005647:Fucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D013270:Stomach / Q000201:enzymology*; D013379:Substrate Specificity
D005647:Fucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; C020559:Glycoprotein 6-alpha-L-fucosyltransferase