On-line assay of the S-enantiomers of enalapril, ramipril and pentopril using a sequential injection analysis/amperometric biosensor system.
A sequential injection analysis/amperometric biosensor system is proposed for the enantioselective analysis of the S-enantiomer of enalapril, ramipril and pentopril. The amperometric biosensor used as detector in the sequential injection analysis was designed by immobilization of l-amino acid oxidase in carbon paste. The proposed SIA system can be utilized reliably for the enantioanalysis of the S-enantiomer from the raw materials as well as from their pharmaceutical formulations, with a rate of 75 samples per hour and R.S.D. values better than 0.1% (n = 10).
Stefan
Raluca-Ioana
RI
Department of Chemistry, University of Pretoria, 0002 Pretoria, South Africa. raluca.stefan@chem.up.ac.za
Staden
Jacobus F van
JF
Bala
Camelia
C
Aboul-Enein
Hassan Y
HY
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004656:Enalapril / Q000032:analysis / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods; D007211:Indoles / Q000032:analysis / Q000737:chemistry*; D009862:Online Systems; D017257:Ramipril / Q000032:analysis / Q000737:chemistry*; D013237:Stereoisomerism
D007211:Indoles; D004656:Enalapril; C038810:CGS 13945; D017257:Ramipril