Influence of beta-carotene on lysosomal hydrolases and their natural substrates in major salivary glands of hamsters treated with 7,12-dimethylbenzanthracene (DMBA).
We evaluated the effects of beta-carotene, a precursor of vitamin A, on the activity of some lysosomal hydrolases and on the levels of their natural substrates in hamster major salivary glands during experimental oral 7,12-dimethylbenzanthracene (DMBA) carcinogenesis. Sixty-four hamsters (Cricetus auratus) were divided into four groups--group 1: untreated control; group 2: DMBA was painted three times a week in the left buccal pouch; group 3: beta-carotene was painted three times a week in the left buccal pouch; group 4: DMBA and beta-carotene were painted alternatively in the left buccal pouch. After 16 weeks, the animals were sacrificed and the activities of some lysosomal hydrolases and their natural substrates in the major salivary glands were measured. beta-Carotene when administered topically in DMBA treated animals (group 4) reduced the levels of the majority of enzymes and substrates closer to those of the untreated control group, thus outlining a mild protective effect of beta-carotene towards the DMBA carcinogenic stress. Nevertheless, the presence of some enzymes which responded negatively to the combined administration of DMBA and beta-carotene suggests the necessity for future studies on the effect of beta-carotene at different concentrations, the systemic administration and the possibility to combine the topical beta-carotene administration with other chemopreventive drugs.
Giuliani
Michele
M
Dental School, Catholic University of Rome-Largo A. Gemelli 8, I-00168 Roma, Italy. michele.giuliani@rm.unicatt.it
Antuzzi
Daniela
D
Lajolo
Carlo
C
Mistura
Lorenza
L
Ricci
Roberta
R
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000633:toxicity*; D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D012469:Salivary Glands / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D019207:beta Carotene / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D002273:Carcinogens; D019207:beta Carotene; D015127:9,10-Dimethyl-1,2-benzanthracene; D006867:Hydrolases