Sulfhydryl based cationic surfactants and the impact of polyanions on disulfide bond formation: implications for gene transfer vectors.
Compacting plasmid DNA (pDNA) into a small size is a fundamental necessity for the efficient in vivo transfer of nucleic acids to somatic cells. An approach for accomplishing this is to condense pDNA using cationic detergents with sulfhydryl groups, near their critical micelle concentration. In this study, a model surfactant was used to study how the rate of disulfide bond formation relates to environmental factors. It was shown that the thiol detergent had the ability to form a disulfide bond when oxidized and the presence of polyanions was significantly increased. The addition of a reducing agent disrupted the disulfide bonds initially, but this was followed by disulfide bond reformation in a short time period.
Fuchs
Scott
S
Department of Pharmaceutics, University of Florida, Gainesville 32610, USA.
Buethe
David
D
Khanna
Anupama
A
Yadava
Preeti
P
Hughes
Jeffrey
J
D002412:Cations; D016264:Dextran Sulfate; D004220:Disulfides / Q000737:chemistry*; D018014:Gene Transfer Techniques; D007093:Imidazoles / Q000737:chemistry; D007848:Laurates / Q000737:chemistry; D008623:Mercaptoethanol / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors
D002412:Cations; D016264:Dextran Sulfate; D004220:Disulfides; D007093:Imidazoles; D007848:Laurates; D008623:Mercaptoethanol; D008823:Micelles; D000071228:Polyelectrolytes; D011108:Polymers; D013438:Sulfhydryl Compounds; D013501:Surface-Active Agents; C499429:N-dodecyl-2-mercaptoimidazole; C499428:methyl 1-imidazolyl laurate