Effect of lipopolysaccharide (LPS) injection on the immune responses of LPS-sensitive mice.
The effect of lipopolysaccharide (LPS) on humoral and cell-mediated immunity was assessed using LPS-sensitive C3H/HeN mice. A single injection of LPS significantly decreased the anti-sheep red blood cells (SRBC) antibody titers, but not the number of anti-SRBC antibody producing spleen cells. In contrast, double LPS injection did not significantly decrease the anti-SRBC titers and even increased the number of anti-SRBC antibody producing spleen cells. Similarly, single LPS injection significantly suppressed the swelling of the footpad, but double LPS injection caused milder suppression. These results suggest that a difference in the level and timing of exposure to LPS may influence the immune response to infection or vaccination.
Ogikubo
Yasuaki
Y
National Veterinary Assay Laboratory, Tokura, Kokubunji, Tokyo, Japan.
Norimatsu
Mari
M
Sasaki
Yoshimasa
Y
Yasuda
Akinori
A
Saegusa
Junzo
J
Tamura
Yutaka
Y
D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice / Q000276:immunology*; D008809:Mice, Inbred C3H; D013154:Spleen / Q000187:drug effects
D000906:Antibodies; D008070:Lipopolysaccharides