Methyl jasmonate induces apoptosis through induction of Bax/Bcl-XS and activation of caspase-3 via ROS production in A549 cells.
Jasmonates are plant lipid derivatives, similar to mammalian eicosanoid, that play a critical role(s) in plant defenses against herbivores and pathogens through up-regulating the expression of defense-related genes. Recently, jasmonates were shown to induce cell cycle arrest or apoptosis in human leukemia, prostate and breast cancer cells, but not in normal lymphocytes, suggesting that the chemicals can be used as a novel class of anti-cancer drugs. In the present study, we examined the molecular mechanism that contributes to methyl jasmonate-induced apoptosis. Herein we show that methyl jasmonate induces apoptosis through induction of Bax/Bcl-XS and activation of caspase-3 via reactive oxygen species production in A549 human lung adenocarcinoma cells.
Kim
Ji Hye
JH
Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, Korea.
Lee
Su Yeon
SY
Oh
Su Young
SY
Han
Song Iy
SI
Park
Hye Gyeong
HG
Yoo
Mi-Ae
MA
Kang
Ho Sung
HS
D000085:Acetates / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D003517:Cyclopentanes / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
D000085:Acetates; C494826:BAX protein, human; C494811:BCL2L1 protein, human; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C072239:methyl jasmonate; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases