title: Microarray analysis of gene expression in the rat vestibular nucleus complex following unilateral vestibular deafferentation.
abstract: To investigate the molecular background of vestibular compensation, a model of lesion-induced plasticity, we used a microarray analysis to examine genes that show asymmetrical expression between the bilateral vestibular nucleus complexes (VNCs) 6 h following unilateral vestibular deafferentation (UVD). Asymmetrical gene expression was then validated by a real-time quantitative PCR. Among the 88 genes for which the ipsilateral (ipsi) : contralateral (contra) was > 1.35, the number of known genes was 33 (38%), and the number of expressed sequence tag (EST) sequences was 55 (62%). Among the 130 genes for which the contra : ipsi was > 1.35, the number of known genes was 55 (42%), and the number of EST sequences was 75 (58%). Changes in some of the genes were consistent with previous studies; however, we found several new genes which could be functionally related to the molecular basis of the electrophysiological asymmetry between the VNCs following UVD. Ipsi > contra genes included the GABA(A) receptor rho subunit, regulatory proteins of G protein signaling, calcium signaling related molecules such as the voltage-dependent calcium channel alpha2/delta subunit 1, calcineurin subunit Abeta and Ca(2+) pump. Contra > ipsi genes included the neuronal high affinity glutamate transporter, 5-hydroxytryptamine receptor 1D, mitogen-activated protein kinase 12 and ubiquitin carboxy-terminal hydrolase L1.
authors.affiliation: Department of Otolaryngology, Osaka University Medical School, Osaka, Japan. ahorii@ent.med.osaka-u.ac.jp
authors.forename: Arata
authors.initials: A
authors.lastname: Horii
authors.forename: Chisako
authors.initials: C
authors.lastname: Masumura
authors.forename: Paul F
authors.initials: PF
authors.lastname: Smith
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Darlington
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitahara
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Uno
authors.forename: Kenji
authors.initials: K
authors.lastname: Mitani
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D003714:Denervation; D007758:Ear, Inner / Q000294:innervation / Q000502:physiology / Q000601:surgery; D020224:Expressed Sequence Tags / Q000737:chemistry; D007839:Functional Laterality / Q000502:physiology; D020869:Gene Expression Profiling; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020710:RGS Proteins / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014726:Vestibular Nuclei / Q000737:chemistry / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000294:innervation / Q000502:physiology* / Q000601:surgery
chemical_list: D002148:Calmodulin-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020710:RGS Proteins; C114095:Strn protein, rat; C116925:regulator of g-protein signaling 9; D019703:Calcineurin