Fluoro-Jade, but not Fluoro-Jade B, stains non-degenerating cells in brain and retina of embryonic and neonatal rats.
Fluoro-Jade (FJ) and Fluoro-Jade B (FJB) are fluorescein derivatives currently used to stain brain cells under degeneration. In this study, we investigated the FJ staining of nondegenerating cells in embryonic and neonatal rat brain and retina. In embryonic rat brain (embryonic day 15; E15), very intense staining of cells was observed. The number of FJ-stained cells and the intensity of staining decreased with increasing in animal age, being almost absent by postnatal day 16 (P16). Only a few cells in neonatal rat brain were in the process of cell death, as verified by the TUNEL technique. The FJ-stained cells in neonatal brain were positive for the neuronal marker neuronal nuclei antigen (NeuN). In retina, FJ stained mainly cells from the ganglion cell layer at P2 and the neuroblastic layer at P2 and P6. In contrast to FJ, FJB did not stain nondegenerating cells in embryonic and neonatal rats. These results show that in addition to staining degenerating brain cells, FJ also stains nondegenerating central nervous system cells in embryonic and neonatal stages.
Fernandes
Anna M A P
AM
Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil.
Maurer-Morelli
Cláudia V
CV
Campos
Cláudia B L
CB
Mello
Maria L S
ML
Castilho
Roger F
RF
Langone
Francesco
F
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D001923:Brain Chemistry; D002470:Cell Survival / Q000502:physiology; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes / Q000032:analysis*; D020287:In Situ Nick-End Labeling; D009410:Nerve Degeneration / Q000378:metabolism*; D009930:Organic Chemicals; D038081:Organogenesis / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000737:chemistry / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D013194:Staining and Labeling
D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade