Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry.
We describe a quantitative phase-imaging interferometer in which phase shifting and noise cancellation are performed by an active feedback loop using a reference laser. Depth gating via low-coherence light allows phase measurement from weakly reflecting biological samples. We demonstrate phase images from a test structure and living cells.
Iwai
Hidenao
H
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Fang-Yen
Christopher
C
Popescu
Gabriel
G
Wax
Adam
A
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D004867:Equipment Design; D019544:Equipment Failure Analysis; D006367:HeLa Cells / Q000473:pathology*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D019047:Phantoms, Imaging; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods