title: Identification of target proteins of N-acetylglucosaminyl-transferase V and fucosyltransferase 8 in human gastric tissues by glycomic approach.
abstract: To understand better the mechanism underlying gastric cancer and search for potential markers for gastric cancer prognosis, the proteomic analysis of gastric cancer tissues was conducted using two-dimensional gel electrophoresis and lectin blot, followed by electrospray ionization-mass spectrometry. These approaches permitted identification of glyco- or putative glycosylated proteins which may be associated with tumorigenesis. The proteins identified include molecules involved in sugar metabolism, signal transduction, proteolysis, and stress, as well as several unknown proteins, which were aberrantly glycosylated as evidenced by the L-phytohemagglutinin blot.
authors.affiliation: Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors.forename: Yong-Sam
authors.initials: YS
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Sejeong
authors.initials: S
authors.lastname: Oh
authors.forename: Hosung
authors.initials: H
authors.lastname: Sohn
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Kang
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Eun-Wie
authors.initials: EW
authors.lastname: Cho
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
authors.forename: Nam-Soon
authors.initials: NS
authors.lastname: Kim
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyoshi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Jeong Heon
authors.initials: JH
authors.lastname: Ko
mesh_terms: D005647:Fucosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D013270:Stomach / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D005647:Fucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; C020559:Glycoprotein 6-alpha-L-fucosyltransferase