title: Improved method to obtain pure alpha-galactosides from lupin seeds.
abstract: Improvement of a previously described method of purification of alpha-galactosides, members of the raffinose family of oligosaccharides (RFOs), has been developed for lupins. The considerable amount of sucrose present in the RFO preparations obtained by the previous method has been removed by modifying the purification stage on diatomaceous earth and charcoal. The present method allows for the preparation of high-purity RFOs containing approximately 99.4% of RFOs in the form of a white fine powder, which provides new perspectives for the production of pure alpha-galactoside preparations for their use as prebiotics in functional foods.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, Madrid 28006, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Villaluenga
authors.forename: Juana
authors.initials: J
authors.lastname: Frias
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gulewicz
authors.forename: Concepción
authors.initials: C
authors.lastname: Vidal-Valverde
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005697:Galactosides / Q000302:isolation & purification*; D031297:Lupinus; D011506:Proteins / Q000737:chemistry*; D011887:Raffinose / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*
chemical_list: D005697:Galactosides; D011506:Proteins; D011887:Raffinose