Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots.
Gram-negative bacteria were isolated from knots induced by Pseudomonas savastanoi in olive trees (Olea europaea L.). A total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. Biochemical characterization indicated that all the strains belong to the family Enterobacteriaceae. Phylogenetic analyses of the 16S rRNA genes of these novel isolates revealed that they formed a homogeneous cluster within Erwinia species. DNA signatures of these isolates were identical to those described for the genus Erwinia. The strains formed a homogeneous group as shown by DNA-DNA hybridization analysis and numerical analysis of phenotypic data, clearly differentiated from all species of Erwinia with validly published names. The data provide strong evidence of the differentiation of these strains from the most closely related species. Therefore, these isolates represent a novel species, for which the name Erwinia toletana sp. nov. is proposed. The isolates are available at CFBP, CECT and ATCC. The G+C content is 52+/-0.5 mol%. The type strain is CFBP 6631(T) (=A37(T)=ATCC 700880(T)=CECT 5263(T)).
Rojas
Ana María
AM
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
Rios
Jose Esteban García de Los
JEGL
Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
Saux
Marion Fischer-Le
MF
UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
Jimenez
Pedro
P
Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
Reche
Paloma
P
Sección de Microbiología, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo, Madrid, Spain.
Bonneau
Sophie
S
UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
Sutra
Laurent
L
UMR de Pathologie Végétale INRA-INH-Université, BP 600 57, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
Mathieu-Daudé
Françoise
F
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
McClelland
Michael
M
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA.
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004885:Erwinia / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D020459:Genes, rRNA; D005840:Gentian Violet; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D031658:Olea / Q000382:microbiology*; D010619:Phenazines; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011549:Pseudomonas / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet