title: Antioxidant effects of water- and lipid-soluble nitroxide radicals in liposomes.
abstract: Liposomes are today useful tools in different fields of science and technology. A lack of stability due to lipid peroxidation is the main problem in the extension of the use of these formulations. Recent investigative works have reported the protective effects of stable nitroxide radicals against oxidative processes in different media and under different stress conditions. Our group has focused its attention on the natural aging of liposomes and the protection provided by the water- and lipid-soluble nitroxide radicals 2,2,6,6-tetramethylpiperdine-1-oxyl (TEMPO) and doxylstearic acids (5-DSA, 12-DSA, and 16-DSA), respectively. Unilamellar liposomes were incubated under air atmosphere at 37 degrees C, both in the absence and in the presence of these radicals. Conjugated dienes, lipid hydroperoxides, TBARS, membrane fluidity, and nitroxide ESR signal intensity were followed as a function of time. Our results demonstrated that doxylstearic acids were more efficient than TEMPO in retarding lipid peroxidation at all the concentrations tested. The inhibition percentages, depending on the total nitroxide concentration, were not proportional to the lipid-water partition coefficient. Furthermore, time-course ESR signals showed a slower decrease for doxylstearic acids than for TEMPO. No significant differences were found among 5-DSA, 12-DSA, and 16-DSA. We concluded that the nitroxide radical efficiency as antioxidant directly depends on both nitroxide concentration and lipophilicity.
authors.affiliation: Cátedra de Física and LANAIS Radicales Libres en Biología y Medicina, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina. lpiehl@ffyb.uba.ar
authors.forename: Alejandra N
authors.initials: AN
authors.lastname: Cimato
authors.forename: Lidia L
authors.initials: LL
authors.lastname: Piehl
authors.forename: Graciela B
authors.initials: GB
authors.lastname: Facorro
authors.forename: Horacio B
authors.initials: HB
authors.lastname: Torti
authors.forename: Alfredo A
authors.initials: AA
authors.lastname: Hager
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D009589:Nitrogen Oxides / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D008055:Lipids; D008081:Liposomes; D009589:Nitrogen Oxides; D014867:Water; D006861:Hydrogen Peroxide; C039900:nitroxyl