title: Bacteriophage Phi S1 infection of Pseudomonas fluorescens planktonic cells versus biofilms.
abstract: This communication focuses on the efficacy of a specific lytic phage, phage Phi S1, as a control agent of Pseudomonas fluorescens biofilms. The effect of phage infection temperature and the host growth temperature were evaluated. The results obtained showed that the phage infection process was temperature dependent and that the optimum temperature of infection of planktonic cells and biofilms was 26 degrees C. At this temperature, bacteriophage Phi S1, at a multiplicity of infection (MOI) of 0.5 infected both planktonic cells and biofilms causing a biomass reduction of about 85% in both cases.
authors.affiliation: Centro de Engenharia Biológica-CEB, Universidade do Minho, Campus de Gualtar 4710-057 Braga, Portugal.
authors.forename: Sanna
authors.initials: S
authors.lastname: Sillankorva
authors.forename: Rosário
authors.initials: R
authors.lastname: Oliveira
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Vieira
authors.forename: Ian W
authors.initials: IW
authors.lastname: Sutherland
authors.forename: Joana
authors.initials: J
authors.lastname: Azeredo
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000254:growth & development / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D018533:Biomass; D002455:Cell Division; D010933:Plankton / Q000166:cytology* / Q000821:virology*; D011551:Pseudomonas fluorescens / Q000166:cytology / Q000254:growth & development* / Q000821:virology*; D013696:Temperature