[Items sterilized in humid heat: validation of the storage system].
We aimed at identifying the period of validity of the sterilization of items by humid heat, taking into account the conditions of sterilization and storage. The validation was carried out by means of microbiological tests performed with instruments in the same load and evaluated in 0, 7, 10, 15, and 25 days. 30% of the instruments in each package were analysed and put in a Mueller Hinton solution and incubated at 37 degrees C for 72 hours. The reading was accomplished taking into consideration how dark the solution was. Out of the samples evaluated in nine loads, none showed microbiological growth. It was concluded that, although sterilization and storage conditions of the material were not fully in accordance with parameters recommended by the literature, the sterilization happened and was maintained for a period of 25 days.
das Neves
Zilah Cândida Pereira
ZC
SCIH do Hospital Materno Infantil e da Coordenação Municipal de DST/AIDS. zilahcandida@hotmail.com
Melo
Dulcelene de Sousa
Dde S
Silva e Souza
Adenícia Custódia
AC
Tipple
Anaclara Ferreira Veiga
AF
Rodrigues
Márcia Alves Vasconcelos
MA
D006358:Hot Temperature; D006813:Humidity; D019064:Product Packaging / Q000592:standards*; D013242:Sterilization / Q000592:standards*