Enzyme-linked immunosorbent assay for bovine apolipoprotein A-IV.
The present report describes an enzyme-linked immunosorbent assay for bovine apolipoprotein (apo) A-IV. This assay was applied to the determination of its concentration and distribution in sera from cattle. The distribution of apoA-IV in lipoprotein fractions separated by ultracentrifugation was mostly recovered in the non-lipoprotein fractions (d>1.21 g/ml, 90%), but, in the case of gel filtration chromatography, apoA-IV was mainly eluted in HDL and non-lipoprotein fractions. The apoA-IV concentrations during early, mid- and late lactating stages in cows were significantly higher than during the nonlactating stage (p<0.05). From early to late lactating stages, the concentration of apoA-IV was unaltered. After 4 days of fasting, the concentration of plasma apoA-IV had decreased significantly (p<0.05) at days 3 and 4, and was returned to the basal level by 3 days of refeeding. These results suggested that the concentration of apoA-IV is modified by nutritional conditions.
Takahashi
Yuji
Y
National Institute of Animal Health, Ibaraki, Japan.
Konishi
Hirotoshi
H
Sato
Kan
K
Oohashi
Tsutai
T
Miyamoto
Toru
T
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001054:Apolipoproteins A / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle / Q000378:metabolism*; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005215:Fasting / Q000378:metabolism*; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014461:Ultracentrifugation
D001054:Apolipoproteins A; C022205:apolipoprotein A-IV