RNA degradation induced by the herpes simplex virus vhs protein proceeds 5' to 3' in vitro.
The herpes simplex virus virion host shutoff protein (vhs) is an mRNA-specific RNase that contributes to shutoff of host protein synthesis. We show here that vhs-induced mRNA decay proceeds 5' to 3' in an in vitro assay system derived from rabbit reticulocyte lysate.
Perez-Parada
Jorge
J
Department of Medical Microbiology & Immunology, 632 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada.
Saffran
Holly A
HA
Smiley
James R
JR
D000818:Animals; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases; D012270:Ribosomes / Q000378:metabolism; D014764:Viral Proteins / Q000502:physiology*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C070722:virion host shutoff protein, Simplexvirus; D012260:Ribonucleases