Early up-regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model.
We investigated cytokine profiles in interleukin (IL)-4 transgenic (Tg) mice with a skin inflammatory disease resembling human atopic dermatitis. cDNA microarray revealed that the mRNAs encoding IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p40, IL-13, tumour necrosis factor (TNF)-alpha, TNF-beta and interferon (IFN)-gamma were up-regulated in the skin of late lesion Tg mice and to a lesser degree in non-lesion Tg mice when compared to those of non-Tg mice. Real time reverse transcription-polymerase chain reaction (RT-PCR) analyses indicated that the cDNA copy numbers of IL-1beta, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma from the skin of late, early and non-lesions increased significantly compared to non-Tg mice. IL-2 and IL-12p40 cDNA copy numbers were increased significantly in early, but not late, lesions. Interestingly, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-10, IL-13, TNF-alpha, and IFN-gamma cDNAs were increased significantly the skin of before-onset and/or non-lesion mice. Flow cytometry analyses demonstrated an increased percentage of keratinocytes producing IL-4 as the disease progressed. The percentage of IL-2, IL-4, IL-10 and IFN-gamma-producing T cells and IL-12-producing antigen-presenting cells in skin-draining lymph nodes and inflammatory skin also increased, particularly in mice with late lesion. These results suggest that disease induction is primarily triggered by Th2 cytokines and that Th1, Th2 and non-Th proinflammatory cytokines are all involved in the disease process.
Chen
L
L
Department of Dermatology, University of Illinois, Chicago, IL 60612, USA.
Martinez
O
O
Overbergh
L
L
Mathieu
C
C
Prabhakar
B S
BS
Chan
L S
LS
D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D016207:Cytokines / Q000276:immunology*; D004247:DNA / Q000032:analysis; D003876:Dermatitis, Atopic / Q000276:immunology*; D004195:Disease Models, Animal; D015847:Interleukin-4 / Q000276:immunology; D007378:Interleukins / Q000276:immunology; D015603:Keratinocytes / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology
D017252:CD3 Complex; D016207:Cytokines; D007378:Interleukins; D012333:RNA, Messenger; D015847:Interleukin-4; D004247:DNA