title: A novel vector for the expression of SCR domains in insect cells.
abstract: Exploitation of recombinant technology to study proteins containing strings of short consensus repeat (SCR) domains largely depends on expression vectors. In this paper, we describe a vector for cloning and constitutive expression of single or multiple SCR domains. The recombinant vector has unique additive features over commercially available vectors that make it a universal cloning vector for SCR domains as well as a vector suitable for expressing any protein fragment beginning and ending with cysteine residues. As a demonstration of its usefulness, the constitutive extracellular expression of five SCR-containing proteins derived from complement factor H is presented.
authors.affiliation: Department of Biochemistry, University of Texas Health Science Center, 11937 U.S. Hwy 271, Tyler, TX 75703, USA.
authors.forename: M Nurul
authors.initials: MN
authors.lastname: Alam
authors.forename: Aftabul
authors.initials: A
authors.lastname: Haque
authors.forename: Maladi
authors.initials: M
authors.lastname: Sreedhar
authors.forename: Michael K
authors.initials: MK
authors.lastname: Pangburn
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D007313:Insecta / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D017434:Protein Structure, Tertiary / Q000235:genetics* / Q000502:physiology; D014162:Transfection
chemical_list: D017242:Complement Factor H