A novel transmembrane MSP-containing protein that plays a role in right ventricle development.
We have identified and characterized a gene, Mospd3 on mouse chromosome 5 using gene trapping in ES cells. MOSPD3 is part of a family of proteins, including MOSPD1, which is defined by the presence of a major sperm protein (MSP) domain and two transmembrane domains. Interestingly Mospd3 is mammalian specific and highly conserved between mouse and man. Insertion of the gene trap vector at the Mospd3 locus is mutagenic and breeding to homozygosity results in a characteristic right ventricle defect and neonatal lethality in 50% of mice. The phenotypic defect is dependent on the genetic background, indicating the presence of genetic modifier loci. We speculate that the further characterization of Mospd3 will shed light on the complex genetic interactions involved in cardiac development and disease.
Pall
Gurman S
GS
Centre for Genome Research, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JQ, UK.
Wallis
Julie
J
Axton
Richard
R
Brownstein
David G
DG
Gautier
Philippe
P
Buerger
Katrin
K
Mulford
Christine
C
Mullins
John J
JJ
Forrester
Lesley M
LM
D000595:Amino Acid Sequence; D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006352:Heart Ventricles / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism*; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D000071164:Trefoil Factor-2
D008565:Membrane Proteins; D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; C481546:TFF2 protein, mouse; D000071164:Trefoil Factor-2