title: [Regulation of pre-mRNA alternative spicing in U251 cells by TPA].
abstract: By using the mini-gene construct containing partial sequence of Bcl-X gene as model, we examined the function of TPA on Bcl-X pre-mRNA alternative splicing in vivo and vitro with RT-PCR and site-directed mutagesis assay. The results show that PKA signaling system can regulate Bcl-X pre-mRNA alternative splicing, the possible mechanism is that the responsible sequence affect the choice of the 5'-downstream or upstream splice site of Bcl-X pre-mRNA.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.
authors.forename: Chang-You
authors.initials: CY
authors.lastname: Li
authors.forename: Jia-You
authors.initials: JY
authors.lastname: Chu
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Liu
authors.forename: Jian-Kun
authors.initials: JK
authors.lastname: Yu
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: ShiI
authors.forename: Yong-Zhang
authors.initials: YZ
authors.lastname: Zhu
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Na
authors.forename: Jiu-Yong
authors.initials: JY
authors.lastname: Xie
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D005419:Flavonoids / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D051020:bcl-X Protein
chemical_list: C494812:Bcl2l1 protein, mouse; D005419:Flavonoids; D019253:Proto-Oncogene Proteins c-bcl-2; D012322:RNA Precursors; D051020:bcl-X Protein; C061097:breviscapine; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate