An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.
General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
Ugahary
Luana
L
The Rotterdam Eye Hospital, Rotterdam, The Netherlands.
van de Sande
Wendy
W
van Meurs
Jan C
JC
van Belkum
Alex
A
D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D009877:Endophthalmitis / Q000175:diagnosis* / Q000382:microbiology; D004866:Equipment Contamination; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D011206:Povidone-Iodine / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry / Q000235:genetics; D014821:Vitrectomy
D000891:Anti-Infective Agents, Local; D004269:DNA, Bacterial; D011206:Povidone-Iodine