Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine.
A selective and sensitive micellar electrokinetic chromatography method with laser-induced fluorescence detection was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. After conducting a series of optimizations, a running buffer of 10 mM sodium borate + 16 mM SDS was used for separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.044-6.6 microg mL(-1) (correlation coefficient: 0.9943 for E, 0.9946 for PE), and the detection limits for E and PE were 0.70 and 0.30 ng mL(-1), respectively. The sensitivity of E and PE was improved by several multiples of ten over those of CZE-LIF method. The method was applied to the analysis of the two alkaloids in ephedra herbal medicine and preparations with recoveries in the range of 98.3-107.1%.
Xie
Jianping
J
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Zhang
Jiyou
J
Liu
Jiaqin
J
Tian
Jianniao
J
Chen
Xingguo
X
Hu
Zhide
Z
D018663:Adrenergic Agents / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D029789:Ephedra / Q000737:chemistry; D004809:Ephedrine / Q000032:analysis*; D007834:Lasers; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D018663:Adrenergic Agents; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D004809:Ephedrine