Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism.
The comprehensive bioconversion of aromatic compounds with a benzene ring to a picolinic acid was achieved with a recombinant Escherichia coli strain that expressed the six genes involved in biphenyl catabolism, these being the bphA1(2072)A2A3A4 genes encoding the evolved biphenyl dioxygenase, the bphB gene encoding dihydrodiol dehydrogenase, and the bphC gene encoding catechol 2,3-dioxygenase.
Shindo
Kazutoshi
K
Department of Food and Nutrition, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan. kshindo@fc.jwu.ac.jp
Osawa
Ayako
A
Nakamura
Ryoko
R
Kagiyama
Yukiko
Y
Sakuda
Shohei
S
Shizuri
Yoshikazu
Y
Furukawa
Kensuke
K
Misawa
Norihiko
N
D001555:Benzene Derivatives / Q000378:metabolism; D001583:Benzoxazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism; D007189:Indans / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010848:Picolinic Acids / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism
D001555:Benzene Derivatives; D001583:Benzoxazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D007189:Indans; D007506:Iron-Sulfur Proteins; D010848:Picolinic Acids; D011804:Quinolines; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C074161:biphenyl-2,3-dioxygenase