Evidence for placental transfer of lipids during gestation in the viviparous lizard, Pseudemoia entrecasteauxii.
During gestation in the viviparous lizard Pseudemoia entrecasteauxii, the fetus obtains nutrients from two sources: uptake of yolk components from the retained egg (lecithotrophy) and transfer of nutrients from the maternal circulation via the placenta (placentotrophy). Although net placentotrophy in this species is indicated by the observation that the neonate contains 1.7 times more dry matter than the egg, the placental transfer of lipid has not been previously demonstrated. Lipid analysis was performed on newly ovulated eggs and on neonates. The weight of total lipid per neonate (8.2+/-0.5 mg) is significantly (P=0.049) greater than that in the egg (6.8+/-0.4 mg), indicating that the placenta must contribute some lipid to the fetus. On the assumption that 50% of the lipid delivered to the fetus from either source is oxidized for energy, it is calculated that the placenta accounts for 58.5% of the fetal lipid requirements, with the remaining 41.5% being derived from the egg. The fatty acid compositions of the triacylglycerol and phospholipid recovered in the neonatal tissue differ substantially from those of the egg. In particular, the proportions of 18:2n-6 and 18:3n-3 are far lower in the neonatal lipids compared with the egg lipids. On the other hand, the proportion of 22:6n-3 in the phospholipid of the neonate is six times higher than in the phospholipid of the egg. The absolute amount (mg) of 22:6n-3 recovered in the total lipid of the neonate is 3.8 times greater than the amount initially present in the egg. By comparison, the amount of total fatty acid in neonatal lipid is 1.2 times greater than the amount in the egg. Thus, there is a preferential use of 22:6n-3 for tissue phospholipid synthesis during development. We conclude that there is net transfer of fatty acids across the placenta to the fetus of P. entrecasteauxii and a high degree of selectivity in the use of the various fatty acids for fetal tissue lipid synthesis.
Speake
Brian K
BK
Animal Health Group, SAC, West Mains Road, Edinburgh, EH9 3JG, UK.
Herbert
Jacquie F
JF
Thompson
Michael B
MB
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000378:metabolism; D004530:Egg Yolk / Q000378:metabolism; D005260:Female; D016014:Linear Models; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008116:Lizards / Q000196:embryology*; D010743:Phospholipids / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D002788:Cholesterol Esters; D010743:Phospholipids; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D008071:Lipoprotein Lipase