title: Gene expression profiling of microdissected pancreatic ductal carcinomas using high-density DNA microarrays.
abstract: Pancreatic ductal adenocarcinoma (PDAC) remains an important cause of malignancy-related death and is the eighth most common cancer with the lowest overall 5-year relative survival rate. To identify new molecular markers and candidates for new therapeutic regimens, we investigated the gene expression profile of microdissected cells from 11 normal pancreatic ducts, 14 samples of PDAC, and 4 well-characterized pancreatic cancer cell lines using the Affymetrix U133 GeneChip set. RNA was extracted from microdissected samples and cell lines, amplified, and labeled using a repetitive in vitro transcription protocol. Differentially expressed genes were identified using the significance analysis of microarrays program. We found 616 differentially expressed genes. Within these, 140 were also identified in PDAC by others, such as Galectin-1, Galectin-3, and MT-SP2. We validated the differential expression of several genes (e.g., CENPF, MCM2, MCM7, RAMP, IRAK1, and PTTG1) in PDAC by immunohistochemistry and reverse transcription polymerase chain reaction. We present a whole genome expression study of microdissected tissues from PDAC, from microdissected normal ductal pancreatic cells and pancreatic cancer cell lines using high-density microarrays. Within the panel of genes, we identified novel differentially expressed genes, which have not been associated with the pathogenesis of PDAC before.
authors.affiliation: Department of Visceral, Thoracic, and Vascular Surgery, University Hospital Carl Gustav Carus, Technical University of Dresden, Dresden, Germany. Robert.Gruetzmann@uniklinikum-dresden.de
authors.forename: Robert
authors.initials: R
authors.lastname: Grützmann
authors.forename: Christian
authors.initials: C
authors.lastname: Pilarsky
authors.forename: Ole
authors.initials: O
authors.lastname: Ammerpohl
authors.forename: Jutta
authors.initials: J
authors.lastname: Lüttges
authors.forename: Armin
authors.initials: A
authors.lastname: Böhme
authors.forename: Bence
authors.initials: B
authors.lastname: Sipos
authors.forename: Melanie
authors.initials: M
authors.lastname: Foerder
authors.forename: Ingo
authors.initials: I
authors.lastname: Alldinger
authors.forename: Beatrix
authors.initials: B
authors.lastname: Jahnke
authors.forename: Hans Konrad
authors.initials: HK
authors.lastname: Schackert
authors.forename: Holger
authors.initials: H
authors.lastname: Kalthoff
authors.forename: Bernd
authors.initials: B
authors.lastname: Kremer
authors.forename: Günter
authors.initials: G
authors.lastname: Klöppel
authors.forename: Hans Detlev
authors.initials: HD
authors.lastname: Saeger
mesh_terms: D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000032:analysis / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D042282:Microdissection; D008840:Microfilament Proteins; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D008840:Microfilament Proteins; D009687:Nuclear Proteins; C094772:centromere protein F; C084859:MCM2 protein, human; C101624:MCM7 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7