Increased megakaryopoiesis in cultures of CD34-enriched cord blood cells maintained at 39 degrees C.
Based on previous evidence suggesting positive effects of fever on in vivo hematopoiesis, we tested the effect of hyperthermia on megakaryopoiesis (MK) in ex vivo cultures of CD34-enriched cord blood (CB) cells. The cells were cultured at 37 degrees C or 39 degrees C for 14 days in cytokine conditions optimized for megakaryocyte development and analyzed periodically. Compared to 37 degrees C, the cultures maintained at 39 degrees C produced significantly more (up to 10-fold) total cells, myeloid and MK progenitors, and total MKs, and showed accelerated and enhanced MK maturation with increased yields of proplatelets and platelets. This observation could facilitate clinical applications requiring ex vivo expansion of hematopoietic cells.
Proulx
Chantal
C
Research and Development, Héma-Québec, 1009, route du Vallon, Sainte-Foy, Québec, Canada G1V 5C3.
Dupuis
Nicolas
N
St-Amour
Isabelle
I
Boyer
Lucie
L
Lemieux
Réal
R
D018952:Antigens, CD34 / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D008533:Megakaryocytes / Q000166:cytology* / Q000502:physiology*; D013696:Temperature
D018952:Antigens, CD34