Optimization and validation of a micellar electrokinetic chromatographic method for the analysis of florfenicol.
We have optimized a micellar electrokinetic capillary chromatographic method for the separation of florfenicol and florfenicol amine, its degradation product. The separation was carried out using a 50mM sodium borate buffer (pH 9.0) containing 25mM of sodium dodecyl sulphate. The method selectivity was proven by the simultaneous separation of florfenicol and two structural antibiotics, chloramphenicol and thiamphenicol. The same system can also be applied for the quantitative determination of these antibiotics. The method was then validated regarding linearity, precision and accuracy.
Hillaert
S
S
Laboratory of Pharmaceutical Chemistry and Drug Analysis, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. sandrahillaert@hotmail.com
Van den Bossche
W
W
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry
C035534:florfenicol; D013839:Thiamphenicol