title: Differential effects of culture conditions on the migration pattern of stromal cell-derived factor-stimulated hematopoietic stem cells.
abstract: Evidence is mounting that hematopoietic stem cells (HSCs) play a critical role in bone marrow regeneration and tissue renewal, for which migration is an obvious prerequisite. Computer-aided analysis and a three-dimensional collagen matrix assay enabled us to analyze single-cell migratory characteristics of stromal cell-derived factor-1 alpha (SDF-1 alpha)-stimulated cord blood-derived HSCs. We defined and resolved specific migratory parameters in spontaneous and SDF-1 alpha-induced migration of these cells. The addition of interleukin 6 to the culture medium led to differential SDF-1 alpha-stimulated migratory response, which comprised a recruitment of nonmoving cells and an increase in speed and frequency of pauses but a decrease in pause duration. We were thus able to decipher the exact parameters that result in an increase in the migration of HSCs and demonstrate that extensive analysis of single-cell behavior is elementary in the study of stem cell migration.
authors.affiliation: Institute of Immunology, University of Witten/Herdecke, Stockumer Str. 10, 58448 Witten, Germany. cweidt@uni-wh.de
authors.forename: Corinna
authors.initials: C
authors.lastname: Weidt
authors.forename: Bernd
authors.initials: B
authors.lastname: Niggemann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hatzmann
authors.forename: Kurt S
authors.initials: KS
authors.lastname: Zänker
authors.forename: Thomas
authors.initials: T
authors.lastname: Dittmar
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002465:Cell Movement; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D018715:Microscopy, Video; D010455:Peptides; D012984:Software; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D006023:Glycoproteins; D015850:Interleukin-6; D008565:Membrane Proteins; D010455:Peptides; C084881:flt3 ligand protein; D003094:Collagen