title: Determination of methylglyoxal in ruminal fluid by high-performance liquid chromatography using fluorometric detection.
abstract: There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at -20 degrees C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 microg/mL was calculated. The recoveries were >80%, and the coefficients of variation were <15%. This method has proven to be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collected from cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced by ruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxal concentration has the potential to be a useful marker to assess ruminal nitrogen status to aid in more accurate diet formulation.
authors.affiliation: Animal and Range Science, New Mexico State University, Las Cruces, New Mexico 88001, USA. sivey@nmsu.edu
authors.forename: Shanna L
authors.initials: SL
authors.lastname: Lodge-Ivey
authors.forename: Tammy
authors.initials: T
authors.lastname: May
authors.forename: Mark K
authors.initials: MK
authors.lastname: Petersen
authors.forename: James R
authors.initials: JR
authors.lastname: Strickland
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004044:Dietary Proteins / Q000008:administration & dosage; D011765:Pyruvaldehyde / Q000032:analysis*; D012417:Rumen
chemical_list: D004044:Dietary Proteins; D011765:Pyruvaldehyde