Detection and selection of microsatellites in the genome of Paracoccidioides brasiliensis as molecular markers for clinical and epidemiological studies.
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.
Nascimento
Erika
E
Departamento de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
Martinez
Roberto
R
Lopes
André Rodrigues
AR
de Souza Bernardes
Luciano Angelo
LA
Barco
Carolina Pomponio
CP
Goldman
Maria Helena S
MH
Taylor
John W
JW
McEwen
Juan G
JG
Nobrega
Marina Pasetto
MP
Nobrega
Francisco G
FG
Goldman
Gustavo H
GH
D000818:Animals; D001135:Armadillos / Q000382:microbiology; D005819:Genetic Markers; D016681:Genome, Fungal; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D010229:Paracoccidioidomycosis / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014774:Virulence
D005819:Genetic Markers