[Inhibition of proliferation and influence of proto-oncogenes expression by matrine in C6 cell].
OBJECTIVE
This study were designed to investigate the inhibition of matrine on C6 glioma cell line and its mechanism.


METHODS
MTT was used to measure the levels of the proliferation of C6 cultured with matrine in different concentrations. The effects of matrine on cell cycle of C6 were observed by FCM. The expression of proto-oncogenes C-myc was measured by RT-PCR.


RESULTS
The proliferation of C6 was obviously inhibited by matrine in a dose dependent manner. The inhibitory rate came to 53.8% +/- 5.6%, when cultured with matrine at 0.20 g/L. The outcome of FCM showed that the proportion of G0/G1 phase cells was decreased obviously, the proportion of S phase cells was reduced when cultured with matrine at 0.20 g/L in 3 days. The outcome of RT-PCR showed that the expression of proto-oncogene C-myc was notably decreased when the dose of matrine was increased.


CONCLUSIONS
Matrine can inhibit the proliferation of C6 and inhibit the expression of proto-oncogenc C-myc.
Deng
Hui
H
College of Pharmacy, Jinan University, Guangzhou 510632.
Luo
Huanmin
H
Huang
Feng
F
Li
Xiaoguang
X
Gao
Qin
Q
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D015870:Gene Expression; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D011807:Quinolizines; D051381:Rats; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D016271:Proto-Oncogene Proteins c-myc; D011807:Quinolizines; D000093842:Matrines