Size-based continuous-flow directional control of DNA with a nano-pillar anisotropic array.
A size-based pre-fractionation chip applicable for enrichment of macromolecules in biological samples is proposed. In this chip, a fractionation unit with a nano-pillar anisotropic array, which functions as sieve meshes, is installed at a bi-forked junction. The mesh sizes are close to the diameters of macromolecules and vary according to the direction to the main and branch exit channels. This fractionation unit provides continuous-flow fractionation without both the dilution of samples and the limitations with the sample loading volume. Additionally, the small channel-volume, which is characteristic of chip-based systems, can reduce sample loss. Using nanometer-scale fabrication technology, we fabricated the chips and demonstrated the flow direction control of DNA molecules.
Hattori
Wataru
W
Fundamental and Environmental Research Laboratories, NEC Corporation, 34 Miyukigaoka, Tsukuba, Ibaraki, Japan. w-hattori@bl.jp.nec.com
Someya
Hiroko
H
Baba
Masakazu
M
Kawaura
Hisao
H
D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D046208:Microchip Analytical Procedures; D036103:Nanotechnology
D004247:DNA