title: Vibrio crassostreae sp. nov., isolated from the haemolymph of oysters (Crassostrea gigas).
abstract: Polyphasic analysis of five new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T)=LMG 22240(T)=CIP 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO(2) production and the presence of the fatty acids 14 : 0 iso and 16 : 0 iso.
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: N
authors.initials: N
authors.lastname: Faury
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: D
authors.initials: D
authors.lastname: Saulnier
authors.affiliation: Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: F L
authors.initials: FL
authors.lastname: Thompson
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: M
authors.initials: M
authors.lastname: Gay
authors.affiliation: Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Swings
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: F Le
authors.initials: FL
authors.lastname: Roux
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D006458:Hemolymph / Q000382:microbiology*; D006867:Hydrolases / Q000032:analysis; D008969:Molecular Sequence Data; D009585:Nitrogen Dioxide / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010125:Ostreidae / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification*; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006867:Hydrolases; D001616:beta-Galactosidase; C019723:arginine deiminase; D027081:DNA Gyrase; D009585:Nitrogen Dioxide