title: Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose.
abstract: OBJECTIVE
  Glucose transporter 4 (GLUT4) is an insulin-responsive glucose transporter expressed in adipose tissue. A decrease of the mRNA abundance of GLUT4 in adipose tissue has been observed in conditions of insulin resistance. The objective was to conduct quantitative genetic analyses using GLUT4 mRNA levels in omental adipose tissue of baboons as a novel phenotype.
  
  
  METHODS
  A blood sample and a biopsy of omental adipose tissue were collected from 418 adult, pedigreed baboons. Total RNA was isolated from adipose tissue biopsies, and GLUT4 mRNA abundance was assayed by quantitative, real-time reverse transcription-polymerase chain reaction. Insulin and glucose were determined in fasting plasma by standard methods. Quantitative genetic analyses were conducted using GLUT4 mRNA, insulin, and glucose as quantitative traits.
  
  
  RESULTS
  GLUT4 mRNA expression in omental adipose tissue was heritable (h(2) = 0.23, p = 0.001). Bivariate genetic analyses revealed a significant genetic correlation (rho(G)) between GLUT4 mRNA abundance and both body weight (rho(G) = 0.63, p = 0.007), BMI (rho(G) = 0.59, p = 0.02) and insulin (rho(G) = 0.72, p = 0.04). A genome scan was conducted, and a quantitative trait locus was detected on chromosome 10p12 with a logarithm of the odds ratio score of 1.1.
  
  
  CONCLUSIONS
  GLUT4 mRNA abundance in omental adipose tissue has a significant genetic component. These findings suggest that expression of GLUT4 mRNA, plasma insulin levels, and body weight may be regulated by common genes.
authors.affiliation: Department of Genetics, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227-5301, USA.
authors.forename: M Elizabeth
authors.initials: ME
authors.lastname: Tejero
authors.forename: J Michael
authors.initials: JM
authors.lastname: Proffitt
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Cole
authors.forename: Jeanne H
authors.initials: JH
authors.lastname: Freeland-Graves
authors.forename: Guowen
authors.initials: G
authors.lastname: Cai
authors.forename: Kyle W
authors.initials: KW
authors.lastname: Peebles
authors.forename: Laura A
authors.initials: LA
authors.lastname: Cox
authors.forename: Michael C
authors.initials: MC
authors.lastname: Mahaney
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Rogers
authors.forename: John L
authors.initials: JL
authors.lastname: Vandeberg
authors.forename: John
authors.initials: J
authors.lastname: Blangero
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Comuzzie
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D051275:Glucose Transporter Type 4; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics*; D010215:Papio; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger