[Nobel genes improve accuracy in detection of peritoneal micro-metastasis of gastric cancer to decide indication for chemotherapy].
Peritoneal metastasis is the most frequent form of recurrence for advanced gastric cancer. We previously performed a global analysis of the gene expression of gastric cancer cell lines established from peritoneal metastasis with cDNA microarray. One of the up-regulated genes is L-3 phosphoserine phosphatase (L3-PP). We have examined its potential as a novel marker for the detection of peritoneal micrometastasis of gastric cancer. L3-PP mRNA in peritoneal wash in 93 gastric cancer patients was quantified for comparison of carcinoembryonic antigen (CEA) mRNA by means of real-time RT-PCR to predict peritoneal recurrence. The quantity of L3-PP and CEA correlated with wall penetration. Eleven out of 18 cases with peritoneal dissemination were L3-PP+ (61% sensitivity). For three out of 18 cases of peritoneal dissemination, only L3-PP could detect micrometastasis of gastric cancer. Consequently, free cancer cells that cannot be detected by CEA mRNA could be detected using L3-PP mRNA. Although CEA alone was not sufficient, L3-PP and CEA in combination can attain a higher accuracy of detection.
Shimomura
Katsumi
K
Dept of Surgery, Kyoto Second Red-Cross Hospital.
Ohgaki
Masaharu
M
Kakihara
Naoki
N
Matsumura
Hiroomi
H
Miyata
Keigo
K
Iizuka
Ryouji
R
Ikawa
Wataru
W
Shimotsuma
Masataka
M
Takenaka
Atsushi
A
Sakakura
Chouhei
C
Hagiwara
Akeo
A
Yamagishi
Hisakazu
H
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000175:diagnosis / Q000188:drug therapy* / Q000556:secondary*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000473:pathology*
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D005819:Genetic Markers; D010744:Phosphoric Monoester Hydrolases; C022394:phosphoserine phosphatase