Lack of association of eNOS (G894T) and p22phox NADPH oxidase subunit (C242T) polymorphisms with systemic sclerosis in a cohort of French Caucasian patients.
OBJECTIVE
To assess the influence of endothelial nitric oxide synthase (eNOS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase polymorphisms on the susceptibility of patients to and clinical expression of systemic sclerosis (SSc).


METHODS
Seventy-seven French Caucasian patients with SSc were studied. Patients and ethnically matched controls (n=49) were genotyped, by restriction enzyme digestion of polymerase chain reaction (PCR) products, for G894T polymorphism in exon 7 of the eNOS gene and for C242T polymorphism of the gene encoding the p22(phox) NADPH oxidase subunit.


RESULTS
The allele and genotype frequencies of the polymorphisms did not differ between patients with SSc and the controls. Moreover, there was no association between these polymorphisms and disease phenotypes.


CONCLUSIONS
Our results indicate that eNOS (G894T) and p22(phox) (C242T) polymorphisms do not influence susceptibility to and the course of systemic sclerosis.
Allanore
Yannick
Y
Department of Rheumatology A, Assistance Publique Hôpitaux de Paris, Cochin Hospital, Paris V University, 75679 Paris Cedex 14, France. yannick.allanore@cch.ap-hop-paris.fr
Borderie
Didier
D
Lemaréchal
Hervé
H
Ekindjian
Ohvanesse Garabed
OG
Kahan
André
A
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015331:Cohort Studies; D004198:Disease Susceptibility; D005602:France; D006801:Humans; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D017354:Point Mutation; D011110:Polymorphism, Genetic; D012595:Scleroderma, Systemic / Q000235:genetics*
C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D019255:NADPH Oxidases