Molecular hydrogen from water radiolysis as an energy source for bacterial growth in a basin containing irradiating waste.
Although being deionized, filtered and therefore normally deeply oligotrophic, the water from a basin containing irradiating waste presented relatively high bacterial concentrations (ca 10(5) cfu ml(-1)) and biofilm development at its surface and on the walls. This water was characterized by a high concentration of molecular H2 due to water radiolysis, while its electrochemical potential was around +400 mV due the presence of dissolved O2 and active oxygen compounds. This combination of H2 availability and of an oxidant environment is completely original and not described in nature. From surface and wall biofilms, we enumerated the autotrophic populations ( approximately 10(5) bacteria ml(-1)) able to grow in presence of H2 as energy source and CO2 as carbon source, and we isolated the most abundant ones among cultivable bacteria. They efficiently grew on a mineral medium, in the presence of H2, O2 and CO2, the presence of the three gases being indispensable. Two strains were selected and identified using their rrs gene sequence as Ralstonia sp. GGLH002 and Burkholderia sp. GGLH005. In pure culture and using isotope exchange between hydrogen and deuterium, we demonstrated that these strains are able to oxidize hydrogen as energy source, using oxygen as an electron acceptor, and to use carbon dioxide as carbon source. These chemoautotroph hydrogen-oxidizing bacteria probably represent the pioneer bacterial populations in this basin and could be primary producers in the bacterial community.
Galès
Grégoire
G
DTN/SMTM/LMTE, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France.
Libert
Marie-Françoise
MF
Sellier
Régine
R
Cournac
Laurent
L
Chapon
Virginie
V
Heulin
Thierry
T
D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D019117:Burkholderia / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004734:Energy Metabolism; D020459:Genes, rRNA / Q000235:genetics; D006859:Hydrogen / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011850:Radioactive Waste; D043367:Ralstonia / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014867:Water / Q000737:chemistry*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011850:Radioactive Waste; D014867:Water; D002245:Carbon Dioxide; D006859:Hydrogen; D010100:Oxygen