title: Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis).
abstract: A mapping referential family (F(1)) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.
authors.affiliation: Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Sendai, Miyagi 981-8555, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hironobu
authors.initials: H
authors.lastname: Fujita
authors.forename: Kimio
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Shingo
authors.initials: S
authors.lastname: Seki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002874:Chromosome Mapping; D018895:Microsatellite Repeats / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D036441:Osmeriformes / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length