title: Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex.
abstract: The Rvb1p and Rvb2p (or TIP48 and TIP49) nuclear ATP binding proteins are universally conserved in eukaryotes and essential for viability of yeasts. Rvbp associate with each other as a double hexamer, with YHR034c and with two complexes involved in chromatin remodeling, Ino80.com and Swr1.com. Loss of Rvb1p or Ino80p affects many yeast promoters similarly. Rvbp are not essential for the recruitment of Ino80p to promoters but are essential for the catalytic activity of Ino80.com. Loss of Rvbp leads to loss of the functionally critical Arp5p in Ino80.com. Rvb2p associates with Arp5p in vitro in a reaction dependent on the presence of ATP and Ino80p. Therefore, Rvbp are required for the structural and functional integrity of the Ino80 chromatin remodeling complex.
authors.affiliation: University of Virginia School of Medicine, Jordan 1240, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA.
authors.forename: Zophonías O
authors.initials: ZO
authors.lastname: Jónsson
authors.forename: Sudhakar
authors.initials: S
authors.lastname: Jha
authors.forename: James A
authors.initials: JA
authors.lastname: Wohlschlegel
authors.forename: Anindya
authors.initials: A
authors.lastname: Dutta
mesh_terms: D051377:Actin-Related Protein 2; D000199:Actins; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002843:Chromatin / Q000235:genetics; D042002:Chromatin Assembly and Disassembly; D004265:DNA Helicases; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D012331:RNA, Fungal / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: C477632:ARP2 protein, S cerevisiae; D051377:Actin-Related Protein 2; D000199:Actins; D002843:Chromatin; C483404:INO80 complex, S cerevisiae; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C428198:RVB1 protein, S cerevisiae; C428199:RVB2 protein, S cerevisiae; D004265:DNA Helicases; D020365:RNA Helicases