title: In vitro screening for antiplasmodial activity of isoquinoline alkaloids from Brazilian plant species.
abstract: In the search for new antimalarial agents, nine Brazilian plant species were selected, from the Annonaceae (6), Menispermaceae (2) and Siparunaceae (1) families naturally occurring at the cerrado and Atlantic rainforest regions, in order to investigate their in vitro antiplasmodial activity. The ethanol and the alkaloid extracts were tested against K1, chloroquine-resistant, and Palo Alto, chloroquine-sensitive, strains of Plasmodium falciparum. The majority of the alkaloid extracts were more active than the ethanol ones, with IC(50) ranging 0.3-8.2 microg/mL. The crude Guatteria australis alkaloids were the most active against K1 with an IC(50) = 0.3 microg/mL. The most promising total alkaloid fractions for further bioguided isolation are those with the IC(50) < or = 5 microg/mL: G. australis, Cissampelos ovalifolia and Duguetia lanceolata.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Bloco15, 05580-900, São Paulo, SP, Brazil. domi@usp.br
authors.forename: Dominique Corinne Hermine
authors.initials: DC
authors.lastname: Fischer
authors.forename: Nilton Carlos
authors.initials: NC
authors.lastname: de Amorim Gualda
authors.forename: Danielle
authors.initials: D
authors.lastname: Bachiega
authors.forename: Cyntia Salerno
authors.initials: CS
authors.lastname: Carvalho
authors.forename: Fernanda Nogueira
authors.initials: FN
authors.lastname: Lupo
authors.forename: Sonia Valéria
authors.initials: SV
authors.lastname: Bonotto
authors.forename: Mariana de Oliveira
authors.initials: Mde O
authors.lastname: Alves
authors.forename: Alvaro
authors.initials: A
authors.lastname: Yogi
authors.forename: Silvia Maria Di
authors.initials: SM
authors.lastname: Santi
authors.forename: Priscilla Elisangela
authors.initials: PE
authors.lastname: Avila
authors.forename: Karin
authors.initials: K
authors.lastname: Kirchgatter
authors.forename: Paulo Roberto Hrihorowitsch
authors.initials: PR
authors.lastname: Moreno
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001938:Brazil; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*
chemical_list: D000470:Alkaloids; D000962:Antimalarials; D007546:Isoquinolines; D010936:Plant Extracts