title: Protective and disease-enhancing immune responses induced by recombinant modified vaccinia Ankara (MVA) expressing respiratory syncytial virus proteins.
abstract: Modified vaccinia Ankara (MVA) recombinants expressing single or multiple RSV surface proteins (F or G) are promising potential vaccines. We studied humoral and cellular responses induced by MVA-F and MVA-G in mice, comparing them to a formalin inactivated RSV preparation (FI-RSV) known to increase disease severity. MVA-F or MVA-G vaccination enhanced weight loss during RSV challenge, but did not show the lung eosinophilia seen after FI-RSV vaccination. FI-RSV induced a stronger total RSV IgG response than the MVA recombinants, but very little IgG2a. MVA recombinants induced cytokine responses biased towards IFNgamma and IL-12, while FI-RSV induced strong IL-4/5 responses in the lungs during RSV challenge. Thus, MVA vaccines induce a favourable immune profile in RSV disease but retain the potential to enhance disease.
authors.affiliation: Department of Respiratory Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College at St Mary's, Paddington, London W2 1PG, UK.
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Olszewska
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Suezer
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
authors.forename: Peter J M
authors.initials: PJ
authors.lastname: Openshaw
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology*; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D007376:Interleukin-2; D022261:Respiratory Syncytial Virus Vaccines; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D007371:Interferon-gamma