[Determination of hyperin in genus Hypericum medicinal plants].
OBJECTIVE
To compare the content of hyperin in Hypericum medicinal plants.


METHODS
HPLC method was established. Alltima C18 radial compression column was used with a mobile phase of methanol-0.5% phosphoric acid (45:55, adjusted to pH = 3.0 with triethylamine). The detecting wavelength was 360 nm. Flow rate was 0.8 ml/min, external standard method was quantitative analysis method.


RESULTS
The linear range was 0.101-2.023 microg. The average recovery was 96.527%. The result showed that the content of hyperin in H. perforatum, H. forrestii was higher than that of others (1.257% and 0.461% respectively).


CONCLUSIONS
This method provides an accurate and sensitive way in detecting hyperin.
Wan
Deguang
D
College of Pharmacy, Chengdu University of TCM, Chengdu 610075.
Pei
Jin
J
Yan
Zhuyun
Z
D002851:Chromatography, High Pressure Liquid; D020902:Hypericum / Q000737:chemistry* / Q000145:classification; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis*; D015203:Reproducibility of Results
C021304:hyperoside; D011794:Quercetin