title: Human skin cell cultures onto PLA50 (PDLLA) bioresorbable polymers: influence of chemical and morphological surface modifications.
abstract: Poly(alpha-hydroxy acid)s derived from lactic and glycolic acid are bioresorbable polymers which can cover a large range of thermal, physical, mechanical, and biological properties. Human keratinocytes have been shown as able to grow on a poly(DL-lactic acid) film. However the keratinocyte growth was delayed with respect to culture on standard tissue culture polystyrene, even though the same plateau level was observed after 2 weeks. In order to improve the performance of poly(DL-lactic acid) films as skin culture support, their surface was modified by creating tiny cavities using a method based on the leaching out of poly(ethylene oxide) from poly(lactic acid)-poly(ethylene oxide) heterogeneous blends. The surface of the films was also chemically modified by alkaline attack with sodium hydroxide and by type-I collagen coating. Murine fibroblast cell line and primary cultures of human fibroblasts and of two types of keratinocytes were allowed to adhere and to grow comparatively on the different films. The presence of cavities affected neither the adhesion of dermal fibroblasts nor that of keratinocytes. Only keratinocyte proliferation was significantly reduced by the presence of cavities. Collagen coating improved skin cell adhesion and proliferation as well, except in the case of murine fibroblasts. In the case of the NaOH treatments, similar trends were observed but their extent depended on the treatment time. In the case of chemical modifications, fluorescence microscopy bore out adhesion and proliferation tendencies deduced from MTT tests.
authors.affiliation: CRBA, UMR CNRS 5473, University Montpellier 1, Faculty of Pharmacy, 15 Avenue Charles Flahault, 34093 Montpellier Cedex 5, France. xgarric@iurc.montp.inserm.fr
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Molès
authors.forename: Henri
authors.initials: H
authors.lastname: Garreau
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Guilhou
authors.forename: Michel
authors.initials: M
authors.lastname: Vert
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D005347:Fibroblasts; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000166:cytology* / Q000187:drug effects*; D012972:Sodium Hydroxide / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011108:Polymers; D014867:Water; D012972:Sodium Hydroxide