title: A new homobifunctional p-nitro phenyl ester coupling reagent for the preparation of neoglycoproteins.
abstract: A new linker system has been designed and applied to neoglycoprotein synthesis. Reaction of oligosaccharide omega-aminoalkyl glycosides with homobifunctional adipic acid p-nitrophenyl diesters in dry DMF gave the corresponding amide half ester in good yields and of sufficient stability to permit chromatographic purification. Subsequent conjugation with bovine serum albumin under very mild conditions afforded the corresponding neoglycoproteins with good efficiency. The method is well suited for the coupling of very small amounts (mg) of oligosaccharide and protein. [structure: see text]
authors.affiliation: Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
authors.forename: Chang-Chun
authors.initials: CC
authors.lastname: Ling
authors.forename: David R
authors.initials: DR
authors.lastname: Bundle
mesh_terms: D000577:Amides; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D006023:Glycoproteins / Q000138:chemical synthesis*; D009596:Nitrophenols / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D000577:Amides; D003432:Cross-Linking Reagents; D004952:Esters; D006023:Glycoproteins; D009596:Nitrophenols; D009844:Oligosaccharides