[Influence of combined microinjection of gene engineering construct and endonuclease on preimplantation development of mouse embryos in vitro].
We studied the influence of combined microinjection of a gene engineering construct and site-specific endonuclease Sal in the pronucleus on preimplantation development of (CBA x C57BL)F1 mouse embryos in vitro. The rate of survival of the embryos was estimated according to their capacity to develop until the blastocyst stage and hatch from zona pellucida. The results obtained suggest that the microinjection of exogenous DNA jointly with endonuclease SalI at concentrations from 0.1 to 0.01 U/microl decreased reliably the rate of survival, as compared to the control (p < 0.05 and p < 0.01, respectively). However, a decrease of endonuclease SalI concentration in the injection mixture to 0.01 U/microl enhanced the capacity of mouse embryos to develop until the blastocyst stage and hatch from zona pellucida, as compared to the embryos microinjected with exogenous DNA and endonuclease SalI at a higher concentration.
Shishimorova
M S
MS
Ivanova
L B
LB
Riabykh
V P
VP
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage / Q000494:pharmacology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000008:administration & dosage / Q000494:pharmacology*; D020964:Embryo Loss / Q000401:mortality; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008845:Microinjections; D011994:Recombinant Proteins; D015044:Zona Pellucida / Q000187:drug effects / Q000648:ultrastructure
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases