title: Gene expression and protein localisation for activin-A, follistatin and activin receptors in goat ovaries.
abstract: We studied the protein and mRNA expression of activin-A, follistatin and activin receptors in goat ovaries to find evidence of their possible role in ovarian activity, particularly in the various stages of follicle development. Ovaries of cyclic goats were collected and then either fixed in paraformaldehyde for immunohistochemical localisation of activin-A, follistatin, activin receptors IIA/B (ActR-IIA/B) and IA (ActR-IA) proteins or used to obtain samples to demonstrate mRNA expression of activin-A (betaA subunit), follistatin, ActR-IIA, -IIB, -IA and -IB, using RT-PCR. For this latter goal, primordial, primary and secondary follicles were isolated mechanically, washed to remove the stromal cells and then used for RT-PCR. In addition, oocytes, cumulus, mural granulosa and theca cells from small (<3 mm) and large (3-6 mm) antral follicles, luteal cells and surface epithelium were collected to study mRNA expression. Activin-A and follistatin proteins were found in oocytes of all follicle classes, granulosa cells from the primary follicle stage onwards, theca cells of antral follicles, corpora lutea and ovarian surface epithelium. In antral follicles, these proteins were detected both in cumulus and mural granulosa cells. ActR-IIA/B protein was found at the same follicular sites, and also in granulosa cells of primordial follicles onward. The localisation of ActR-IA corresponded with that of ActR-IIA/B, but the former protein was absent in the theca of large antral follicles. The mRNAs for activin-A (betaA subunit), follistatin, and ActR-IIA, -IIB, -IA and -IB were detected at all follicular and cellular types studied, except that ActR-IIB was not found in follicles that had not developed an antrum yet. In conclusion, in goat ovaries, transcripts of activin-A (betaA subunit), its receptors and its binding protein follistatin are expressed and their proteins formed at all follicular stages and in corpora lutea. These findings indicate a role of activin-A in the local regulatory system during the entire follicular development and during luteal activity.
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. j.r.v.silvia@vet.uu.nl
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.forename: R
authors.initials: R
authors.lastname: van den Hurk
authors.forename: H T A
authors.initials: HT
authors.lastname: van Tol
authors.forename: B A J
authors.initials: BA
authors.lastname: Roelen
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D029404:Activin Receptors / Q000032:analysis* / Q000235:genetics; D030301:Activin Receptors, Type II / Q000032:analysis / Q000235:genetics; D028341:Activins / Q000032:analysis* / Q000235:genetics; D000818:Animals; D003338:Corpus Luteum / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D030762:Estrous Cycle; D005260:Female; D038681:Follistatin / Q000032:analysis* / Q000235:genetics; D006041:Goats; D006107:Granulosa Cells / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods; D028322:Inhibin-beta Subunits / Q000032:analysis* / Q000235:genetics; D009865:Oocytes / Q000737:chemistry; D010053:Ovary / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013799:Theca Cells / Q000737:chemistry
chemical_list: D038681:Follistatin; D011506:Proteins; D012333:RNA, Messenger; C422405:activin A; D028341:Activins; D028322:Inhibin-beta Subunits; D029404:Activin Receptors; D030301:Activin Receptors, Type II; C425585:activin receptor type II-A; C425584:activin receptor type II-B