title: [A new method for phylogenic analysis].
abstract: With ESTs from porcine fatty tissue and cDNA sequences from human, bovine and mouse in non-reduncdant database and dbEST in GenBank,we sampled cDNA sequences of 70 function-known genes in four species on the base of randomly sampling method, analyzed the mutation pattern of 70 x 150 bp linking sequences between them, and established an integrated phylogenic analysis method. The results showed that 391 single bases mutations were found in 70 x 150 bp linking sequences alignment among four species. The number of mutation bases between them were greatly exceeded the 1/1000 predicted in the human genome analysis. C/T(T/C) and A/G (G/A) transitions were the major types of single base mutation. The genetic relationship between pig and bovine who are both Artiodactylous is the nearest, the next is human, and the farthest is mouse. The differentiation sequence taken place in four species from the same ancestor is that mouse is the earliest one, and the latter human, and pig and bovine are the latest.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China. zh65zh@sina.com.cn
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Fan
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Deng
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Hu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D020224:Expressed Sequence Tags; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine