title: Casuarinin protects cultured MDCK cells from hydrogen peroxide-induced oxidative stress and DNA oxidative damage.
abstract: Casuarinin has been shown to be an antioxidant in acellular experiments. This study was designed to assess the ability of casuarinin, extracted from Terminalia arjuna, to protect cultured Madin-Darby canine kidney (MDCK) cells against H2O2-mediated oxidative stress. A comparison with trolox, a hydrosoluble vitamin E analogue was performed. MDCK cells were pretreated with casuarinin or trolox for 1 h, then exposed to H2O2. After incubation with 0.8 mM H2O2 for 1 h, casuarinin caused a decrease in intracellular peroxide production as shown by dichlorofluorescein (DCF) fluorescence in a concentration-dependent manner. After 3 h exposure to 8 mM H2O2, the percentage of intracellular glutathione (GSH)-negative cells was reduced in the casuarinin-treated group. Addition of 32mM H2O2 to MDCK cells for 3 h induced an increase in the percentage of cells containing 8-oxoguanine but the level of such cells declined in casuarinin-treated cells. These results show that casuarinin is more effective against H2O2-induced oxidative damage than trolox. The data suggest that casuarinin attenuates H2O2-induced oxidative stress, decreases DNA oxidative damage and prevents the depletion of intracellular GSH in MDCK cells.
authors.affiliation: Department of Medical Technology, Fooyin University, Ta-Liao, Kaohsiung Hsien, Taiwan, ROC.
authors.forename: Ching-Hsein
authors.initials: CH
authors.lastname: Chen
authors.forename: Tsan-Zon
authors.initials: TZ
authors.lastname: Liu
authors.forename: Tsun-Cheng
authors.initials: TC
authors.lastname: Kuo
authors.forename: Fung-Jou
authors.initials: FJ
authors.lastname: Lu
authors.forename: Yu-Chin
authors.initials: YC
authors.lastname: Chen
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chang-Chien
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D029766:Terminalia
chemical_list: D000975:Antioxidants; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C472513:casuarinin; D006861:Hydrogen Peroxide; D005978:Glutathione