title: 'Candidatus Burkholderia calva' and 'Candidatus Burkholderia nigropunctata' as leaf gall endosymbionts of African Psychotria.
abstract: Phylogenetic 16S rRNA gene analysis was used to assign the bacterial leaf-nodulating endosymbionts of two tropical African Psychotria species to the genus Burkholderia. The microsymbionts of the different Psychotria hosts were recognized as distinct and novel species of Burkholderia on the basis of relatively low intersequence similarities and sufficiently large evolutionary distances when compared with each other and their closest validly named neighbours. The obligate endosymbiotic nature of the bacteria prevented their in vitro cultivation and the deposition of type strains to culture collections. Therefore, the provisional status Candidatus is assigned to the bacterial partners of Psychotria calva and Psychotria nigropunctata, with the proposal of the names 'Candidatus Burkholderia calva' and 'Candidatus Burkholderia nigropunctata', respectively.
authors.affiliation: Laboratory of Plant Biochemistry and Physiology, Department of Biology, Antwerp University, Campus Drie Eiken, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Van Oevelen
authors.affiliation: Professor Emeritus, Laboratory of Molecular Biology, Department of Biochemistry, Antwerp University, Campus Drie Eiken, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Rupert
authors.initials: R
authors.lastname: De Wachter
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.affiliation: National Botanic Garden, Domein van Bouchout, B-1860 Meise, Belgium.|Laboratory of Plant Biochemistry and Physiology, Department of Biology, Antwerp University, Campus Drie Eiken, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Elmar
authors.initials: E
authors.lastname: Robbrecht
authors.affiliation: Laboratory of Plant Biochemistry and Physiology, Department of Biology, Antwerp University, Campus Drie Eiken, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Prinsen
mesh_terms: D019117:Burkholderia / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D032069:Psychotria / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S