Interleukin 4 increases the antibody response against Rubisco in mice.
The influence of interleukin 4 (IL-4) on antibody titer in serum and spleen culture supernatant in mice immunized with spinach (Spinacia oleracea L.) Rubisco was investigated. Therefore, we boosted one mouse additionally to the antigen with recombinant mouse IL-4. We found that the Rubisco-specific antibody titer in serum as well as in spleen cell culture supernatant was significantly enhanced in the IL-4 mouse. Most of the antibodies were of the IgG1 subclass. After hybridoma generation, Rubisco-specific antibodies were found in more than 95% of the wells tested compared to about 12% of the control mouse.
Schenk
Jörg A
JA
University of Potsdam, Institute of Biochemistry and Biology, Department of Biotechnology, D-14476 Golm, Germany. jschenk@mdc-berlin.de
Matyssek
Franziska
F
Micheel
Burkhard
B
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002999:Clone Cells; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012273:Ribulose-Bisphosphate Carboxylase / Q000008:administration & dosage* / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007074:Immunoglobulin G; D015847:Interleukin-4; D012273:Ribulose-Bisphosphate Carboxylase