Structural and serological characterization of the major glycolipid from Rothia mucilaginosa.
Structural studies on the major glycolipid isolated from Rothia mucilaginosa were carried out utilising specific chemical degradation, NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI TOF-MS). The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside alpha-D-Manp-(1-->3)-alpha-D-Manp-(1-->3)-sn-Gro (Man A-Man B-Gro), of which Man B was esterified at O-6 by a fatty acid residue. A second fatty acid substituted the secondary methylene position of the glycerol residue, in contrast to the glycolipid previously found in R. dentocariosa and Saccharopolyspora strains, in which the second fatty acid esterified the primary methylene position of glycerol. Results of the ELISA experiment with rabbit specific antibacterial sera indicate that these two major glycolipids are antigenic, and the patterns of serological reactivity are similar but not identical.
Paściak
Mariola
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, PL-53-114 Wrocław, Poland.
Holst
Otto
O
Lindner
Buko
B
Mierzchała
Magdalena
M
Grzegorzewicz
Anna
A
Mordarska
Halina
H
Gamian
Andrzej
A
D000818:Animals; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008835:Micrococcaceae / Q000737:chemistry*; D015394:Molecular Structure; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006017:Glycolipids; C031647:dimannosyl diacylglycerol