Reactivity of anti-Nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental Nipah and Hendra virus infections.
The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue. Two clones (11F6 and 13A5) reacted with Nipah virus-infected porcine lung tissue, but not with Hendra virus-infected equine lung tissue. These Nipah virus-specific monoclonal antibodies may therefore be useful for immunohistological diagnosis of Nipah virus infection and for further research on Nipah virus pathogenesis.
Tanimura
Nobuhiko
N
National Institute of Animal Health, Ibaraki, Japan.
Imada
Tadao
T
Kashiwazaki
Yoshihito
Y
Shamusudin
Shahirudin
S
Syed Hassan
Sharifah
S
Jamaluddin
Aziz
A
Russell
Gail
G
White
John
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D045464:Henipavirus Infections / Q000175:diagnosis / Q000276:immunology*; D006736:Horses; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D045405:Nipah Virus / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral