Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity.
A novel alkaliphile was isolated from a drain of a fish processing plant. The isolate grew at a pH range of 7-10. Cells were Gram-positive, facultatively aerobic, motile rods with peritrichous flagella. Colonies were orange or yellow in colour. Catalase and oxidase reactions were positive. The isolate grew in 0-12 % NaCl but not above 15 % NaCl. Its cell extract exhibited 567 times higher catalase activity than an Escherichia coli cell extract. The major cellular fatty acids were iso-C(13 : 0), anteiso-C(13 : 0), iso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0), anteiso-C(17 : 0) and iso-C(17 : 1). Its DNA G+C content was 46.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing and chemotaxonomic data indicated that strain T-2-2(T) is a member of the genus Exiguobacterium. DNA-DNA hybridization revealed a low relatedness of the isolate to several phylogenetic neighbours (less than 25 %). On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Exiguobacterium oxidotolerans sp. nov. is proposed. The type strain is T-2-2(T) (=JCM 12280(T)=NCIMB 13980(T)).
Yumoto
Isao
I
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Hishinuma-Narisawa
Megumi
M
National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Hirota
Kikue
K
National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Shingyo
Tomohiro
T
Hokkaido Tokai University, Minaminosawa, Minami-ku, Sapporo 005-8601, Japan.|National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Takebe
Fumihiko
F
Hokkaido Tokai University, Minaminosawa, Minami-ku, Sapporo 005-8601, Japan.|National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Nodasaka
Yoshinobu
Y
School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
Matsuyama
Hidetoshi
H
Hokkaido Tokai University, Minaminosawa, Minami-ku, Sapporo 005-8601, Japan.
Hara
Isao
I
National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
D000332:Aerobiosis; D001406:Bacillaceae / Q000145:classification* / Q000166:cytology / Q000201:enzymology / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005407:Flagella; D020459:Genes, rRNA; D005840:Gentian Violet; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010619:Phenazines; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D012722:Sewage; D010088:Oxidoreductases; D002374:Catalase; D005840:Gentian Violet