title: Circulating levels of macrophage migration inhibitory factor are associated with mild pulmonary dysfunction after cardiopulmonary bypass.
abstract: Macrophage migration inhibitory factor (MIF) is a central mediator of inflammatory response and acute lung injury that is secreted in response to corticosteroids. A rise in systemic MIF levels was described after cardiac surgery in steroid-treated patients. This study aimed to investigate the circulating levels of MIF and the possible relationship of this cytokine to pulmonary dysfunction after cardiopulmonary bypass (CPB). We included 74 patients without previous organ dysfunction undergoing elective coronary artery bypass surgery (CABS). The same team performed all CABS via a standard technique adding methylprednisolone (15 mg/kg) to the CPB priming solution (Group MP, n = 37). In the remaining patients (Group NS, n = 37), methylprednisolone was withdrawn from the CPB priming. MIF, C-reactive protein (CRP), and total C3 were assayed in peripheral blood sampled immediately before anesthesia induction and 3, 6, and 24 h post-CPB. Preoperative risk scores and peri- and postoperative variables were documented. Postoperative kinetics of MIF and C3 were similar for both groups. Levels of CRP 24 h post-CPB were higher in Group MP (P = 0.003). Higher MIF levels were detected 6 h post-CPB, and returned to preoperative levels 24 h after CPB. MIF levels 6 h post-CPB were inversely related to the postoperative PaO2/FiO2 ratio (P = 0.0021) and were directly related to the duration of mechanical ventilation (P = 0.014). Perioperative use of methylprednisolone did not modify the MIF response to CPB, but it was related to an enhanced acute phase response. Higher circulating MIF levels 6 h post-CPB were associated with worse postoperative pulmonary short-course outcome.
authors.affiliation: Laboratory of Immunopharmacology, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. imunobiologia@procardiaco.com.br
authors.forename: Hugo Tannus Furtado
authors.initials: HT
authors.lastname: de Mendonça-Filho
authors.forename: Renato Vieira
authors.initials: RV
authors.lastname: Gomes
authors.forename: Luis Antonio
authors.initials: LA
authors.lastname: de Almeida Campos
authors.forename: Bernardo
authors.initials: B
authors.lastname: Tura
authors.forename: Edson Magalhães
authors.initials: EM
authors.lastname: Nunes
authors.forename: Rachel
authors.initials: R
authors.lastname: Gomes
authors.forename: Fernando
authors.initials: F
authors.lastname: Bozza
authors.forename: Patricia T
authors.initials: PT
authors.lastname: Bozza
authors.forename: Hugo Caire
authors.initials: HC
authors.lastname: Castro-Faria-Neto
mesh_terms: D015415:Biomarkers / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008168:Lung / Q000503:physiopathology*; D008263:Macrophage Migration-Inhibitory Factors / Q000097:blood*; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology / Q000627:therapeutic use; D011183:Postoperative Complications; D012123:Pulmonary Ventilation; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D008263:Macrophage Migration-Inhibitory Factors; D008775:Methylprednisolone