Changes of phospholipid composition within the dystrophic muscle by matrix-assisted laser desorption/ionization mass spectrometry and mass spectrometry imaging.
Duchenne muscular dystrophy (DMD) is a neuromuscular disease linked to the lack of the dystrophin, a submembrane protein, leading to muscle weakness and associated with a defect of the lipid metabolism. A study of the fatty acid composition of glycerophosphatidylcholines by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) and tandem mass spectrometry (MS/MS) enabled us to characterize a change of the lipid composition of dystrophic cells at the time of the differentiation. This modification has been used as a marker to identify with profiling and imaging MALDI-ToF MS regenerating areas in sections of an mdx mouse leg muscle. It is the first time that such a slight change in fatty acid composition has been observed directly on tissue slices using mass spectrometry. This approach will be useful in monitoring the treatment of muscular regeneration.
Touboul
David
D
Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, CNRS, 1 Avenue de la Terrasse, F91198 Gif sur Yvette Cedex, France.
Piednoël
Hélène
H
Voisin
Vincent
V
De La Porte
Sabine
S
Brunelle
Alain
A
Halgand
Frédéric
F
Laprévote
Olivier
O
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000032:analysis; D005997:Glycerylphosphorylcholine / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D020388:Muscular Dystrophy, Duchenne / Q000378:metabolism; D032446:Myoblasts / Q000737:chemistry / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015415:Biomarkers; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; C002449:glycerophosphoethanolamine; D005997:Glycerylphosphorylcholine