title: Pharmacological properties and functional role of a TRP-related ion channel in lobster olfactory receptor neurons.
abstract: Odors activate lobster olfactory receptor neurons (ORNs) through phosphoinositide signaling that appears to target a Na(+)-gated nonselective cation channel. The Na(+)-gated channel is a potential member of the growing family of transient receptor potential (TRP) channels. Here, we test the effect of potential antagonists on the channel in cell-free patches from cultured lobster ORNs. We show that the channel is antagonized by H+ and the TRP channel blockers 2-aminoethoxydiphenyl borate, SKF96365, ruthenium red, Al3+, Gd3+, and La3+. We then use this enhanced antagonist profile together with the agonists Na+ and Ca2+ to implicate the channel in signal amplification in the cells.
authors.affiliation: Whitney Laboratory for Marine Bioscience, University of Florida, 9505 Ocean Shore Blvd, St. Augustine, FL 32080-8610, USA. bobkov@whitney.ufl.edu)
authors.forename: Yuriy V
authors.initials: YV
authors.lastname: Bobkov
authors.forename: Barry W
authors.initials: BW
authors.lastname: Ache
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002412:Cations / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004558:Electric Stimulation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000502:physiology*; D033521:Palinuridae / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D011336:Probability; D012965:Sodium Chloride / Q000494:pharmacology; D050052:TRPC Cation Channels
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D002412:Cations; D007093:Imidazoles; D007473:Ion Channels; D050052:TRPC Cation Channels; D012965:Sodium Chloride; C063159:1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole; D002118:Calcium