[In vivo and in vitro bone regeneration from cord blood derived mesenchymal stem cells].
BACKGROUND
Mesenchymal stem cells with an osteoblastic differentiating potency are investigated in regard of probable tissue engineering for further clinical application. The following report describes the use of cord blood derived stem cells as an alternative to other stem cell populations for bone regenerating tissue engineering.


METHODS
To demonstrate the multipotency of cord blood derived mesenchymal stem cells, unrestringated somatic stem cells (USSC) were isolated from cord blood and underwent an osteo-, chondro- and adipoblastic in vitro stimulation. To evaluate the osteoinductive potency of a porcine collagen I/III cell carrier USSC were incubated on this matrix. To investigate the in vivo effects of human USSC an athymic rat model was developed. These cells were transplanted into a femoral defect.


RESULTS
Cord blood derived mesenchymal stem cells (USSC) have an in vitro multipotency and show adipo-, chondro- and osteogenic differentiation. The porcine collagen I/III carrier promoted an osteoblastic differentiation. USSC survived after xenotransplantation in an athymic rat and differentiated into osteoblasts filling the bony defect zone.


CONCLUSIONS
Human USSC are a mesenchymal multipotent stem cell population that shows osteoblastic differentiation onto a collagen I/III carrier in vitro as well as in an athymic rat in vivo.
Jäger
M
M
Orthopädische Universitätsklinik, Heinrich-Heine-Universität , Düsseldorf. Jaeger@med.uni-duesseldorf.de
Sager
M
M
Knipper
A
A
Degistirici
O
O
Fischer
J
J
Kögler
G
G
Wernet
P
P
Krauspe
R
R
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005264:Femoral Fractures / Q000473:pathology / Q000601:surgery; D005269:Femur / Q000473:pathology / Q000601:surgery; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin; D051381:Rats; D011923:Rats, Nude; D012795:Sialoglycoproteins / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous
C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin