title: Evaluation of oxidative stress markers for the early diagnosis of allograft rejection in feline renal allotransplant recipients with normal renal function.
abstract: The purpose of this study was to identify oxidative damage to renal allografts during graft rejection by evaluating changes in oxidative markers and plasma lactate levels in feline renal allotransplant recipients. Heterotopic renal allotransplantations were performed between 8 adult feline cross-matched donors. Following 14 d of immunosuppression, the drugs were discontinued to allow allograft rejection. Baseline and serial postoperative evaluations of serum creatinine, plasma lactate, plasma thiobarbituate reactive substances (TBARS), plasma creatol, urine creatol, and renal sonographic cross-sectional area were performed. When sonographic evaluation revealed the absence of blood flow to the allograft, the rejected kidney was nephrectomized and evaluated histopathologically. Allograft rejection occurred in all cats by day 26. A significant elevation in body temperature occurred during the rejection period. No significant change was observed between any of the time periods for plasma TBARS, creatol, or urine creatol. There was a significant decrease in plasma lactate levels throughout the study. Markers of oxidative stress from venous blood did not reflect renal allograft rejection in cats with a normally functioning native kidney. Renal allograft rejection may be associated with significant increases in body temperature and warrants further investigation.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610, USA.
authors.forename: Krista B
authors.initials: KB
authors.lastname: Halling
authors.forename: Gary W
authors.initials: GW
authors.lastname: Ellison
authors.forename: Don
authors.initials: D
authors.lastname: Armstrong
authors.forename: Kasumi
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Detrisac
authors.forename: John P
authors.initials: JP
authors.lastname: Graham
authors.forename: Susan P
authors.initials: SP
authors.lastname: Newell
authors.forename: Frank G
authors.initials: FG
authors.lastname: Martin
authors.forename: James M
authors.initials: JM
authors.lastname: Van Gilder
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002371:Cat Diseases / Q000601:surgery; D002415:Cats; D003404:Creatinine / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D005334:Fever / Q000662:veterinary; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007668:Kidney / Q000098:blood supply / Q000000981:diagnostic imaging; D007676:Kidney Failure, Chronic / Q000601:surgery / Q000662:veterinary; D007677:Kidney Function Tests / Q000379:methods / Q000662:veterinary; D016030:Kidney Transplantation / Q000662:veterinary*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D013047:Specific Pathogen-Free Organisms; D017392:Thiobarbituric Acid Reactive Substances; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; C064402:creatol; D019344:Lactic Acid; D003404:Creatinine