Isolation and characterization of a fucoidan-degrading marine bacterial strain and its fucoidanase.
A marine bacterial strain that degraded fucoidan from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) was isolated in our laboratory. The strain was gram-negative, ubiquinone 8 was the predominant respiratory quinone, and the GC-content of its genomic DNA was 36%. The cells of the strain were rod-shaped (2.0 microm long x 1.0 microm wide), and each cell was motile by means of one polar flagellum. Phylogenetic analysis of its 16S ribosomal DNA sequence indicated that it was a member of the family Alteromonadaceae. It produced a type of extracellular fucoidanase, an endosulfated fucan-digesting enzyme. The enzyme was purified with 3500-fold purity at 12.0% yield. Optimum conditions for the enzyme reaction were approximately pH 6.5 to 8.0 and temperature 30 degrees to 35 degrees C. The enzyme was activated by calcium ions, and maximum activity was observed in the presence of greater than 30 mM calcium ion.
Sakai
Takeshi
T
Takara Bio Inc., Biotechnology Research Laboratories, Seta 3-4-1, Otsu-shi, Shiga-ken 520-2193, Japan. sakait@takara-bio.co.jp.
Kawai
Takashi
T
Kato
Ikunoshin
I
D034241:Alteromonadaceae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D004798:Enzymes / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000378:metabolism*; D010802:Phylogeny; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
D017931:DNA Primers; D004798:Enzymes; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; C007789:fucoidan