title: Ultrastructural and immunohistochemical similarities of two distinct entities; multiple sclerosis and hereditary motor sensory neuropathy.
abstract: In the present study, we present the ultrastructural and immunohistochemical properties of the sural nerves of two patients, one of whom was diagnosed as having multiple sclerosis with involvement of the peripheral nervous system (PNS), and the other as having hereditary motor sensory neuropathy type-I with involvement of the central nervous system (CNS). Expression of several extracellular matrix (ECM) proteins (fibronectin, laminin, and collagen type-IV), intermediate filaments (vimentin) and S-100 protein (marker for the axon-Schwann cell interface) was investigated by means of immunohistochemical methods. In addition, the tissue samples were evaluated ultrastructurally. Immunohistochemical staining revealed increased expression of the ECM molecules mentioned above in relation with the sural nerves of the patients. We hypothesize that this enhanced expression is due to Schwann cell-axon interactions. Vimentin expression was different in Schwann cells and S-100 immunostaining was decreased near the Schwann cell-axon interface. Myelin fragmentation, axon vacuolization, onion bulbs, tomoculous formation, axonal degeneration were found to occur. These results suggest that there is active ECM reorganization in the sural nerve of these patients, and some ultrastructural changes are similar in the damaged axonal organization and in Schwann cells although the changes are not completely the same in the two patients. In conclusion, our study demonstrates that there is an association between the demyelinization process in the CNS and the PNS even though they are affected by different mechanisms.
authors.affiliation: Department of Neurology, Medical School, Dokuz Eylül University, Izmir, Turkey.
authors.forename: Goksemin
authors.initials: G
authors.lastname: Acar
authors.forename: Gamze
authors.initials: G
authors.lastname: Tanriover
authors.forename: Necdet
authors.initials: N
authors.lastname: Demir
authors.forename: Umit A
authors.initials: UA
authors.lastname: Kayisli
authors.forename: G Leyla
authors.initials: GL
authors.lastname: Sati
authors.forename: Aylin
authors.initials: A
authors.lastname: Yaba
authors.forename: Egemen
authors.initials: E
authors.lastname: Idiman
authors.forename: Ramazan
authors.initials: R
authors.lastname: Demir
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002607:Charcot-Marie-Tooth Disease / Q000378:metabolism / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D046529:Microscopy, Electron, Transmission; D009103:Multiple Sclerosis / Q000378:metabolism / Q000473:pathology*; D017933:Peripheral Nervous System / Q000378:metabolism / Q000473:pathology*; D013497:Sural Nerve / Q000648:ultrastructure*
chemical_list: D015415:Biomarkers