title: New polymorphism of FASN gene in chicken.
abstract: Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.
authors.affiliation: Genetics Area, Animal Production Department, Veterinary School, University of Buenos Aires, Chorroarin 280, 1427, Buenos Aires, Argentina. gmarrube@fvet.uba.ar
authors.forename: Graciela
authors.initials: G
authors.lastname: Marrube
authors.forename: Felisa
authors.initials: F
authors.lastname: Rozen
authors.forename: Gabriel Bernardo
authors.initials: GB
authors.lastname: Pinto
authors.forename: Natalia
authors.initials: N
authors.lastname: Pacienza
authors.forename: Julián Enrique
authors.initials: JE
authors.lastname: Melo
authors.forename: Miguel Javier
authors.initials: MJ
authors.lastname: Huguet
authors.forename: Zulma
authors.initials: Z
authors.lastname: Canet
authors.forename: Ruben
authors.initials: R
authors.lastname: Zandomeni
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Miquel
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D064429:Fatty Acid Synthases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D007438:Introns / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D064429:Fatty Acid Synthases