title: Identification of a novel non-carbohydrate molecule that binds to the ribosomal A-site RNA.
abstract: We report the identification of a novel compound that binds to the Escherichia coli 16S ribosomal A-site. Binding by the compound was observed using nuclear magnetic resonance and mass spectrometry techniques. We show that the compound binds in the same position in the A-site RNA as occupied by the aminoglycoside class of antibiotics.
authors.affiliation: MCR Research Inc., Toronto, Ontario, Canada, M3J 1P3. shawnm@nrxn.com
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Maddaford
authors.forename: Mina
authors.initials: M
authors.lastname: Motamed
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Turner
authors.forename: Min Soo K
authors.initials: MS
authors.lastname: Choi
authors.forename: Jailall
authors.initials: J
authors.lastname: Ramnauth
authors.forename: Suman
authors.initials: S
authors.lastname: Rakhit
authors.forename: Robert R
authors.initials: RR
authors.lastname: Hudgins
authors.forename: Daniele
authors.initials: D
authors.lastname: Fabris
authors.forename: Philip E
authors.initials: PE
authors.lastname: Johnson
mesh_terms: D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000737:chemistry; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011759:Pyrrolidines / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D011759:Pyrrolidines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S