Kinetic resolution of ketoprofen ester catalyzed by lipase from a mutant of CBS 5791.
A biotransformation process was developed for the production of (S)-ketoprofen by enantioselective hydrolysis of racemic ketoprofen ester using the mutant Trichosporon laibacchii strain CBS 5791. A satisfactory result was obtained, in which the E was 82.5, with an ee of 0.94 and a conversion of 0.47 under the optimum hydrolysis conditions [E is enantiomeric ratio, E=ln[1-X(1+ee)]/ln[1-X(1-ee)]; ee is enantiomeric excess, ee=(CS-CR)/(CS+CR): temperature of hydrolysis was 23 degrees C]. The medium used in biotransformation was a mixture of growth broth and biotransformation broth at a ratio of 1:9, the concentration of Tween 80 was 15 g/l, the time of hydrolysis, 72 h. These results are promising for further scale-up. Tween 80 significantly improved lipase enantioselectivity and activity at the optimum concentration.
Liu
Junhong
J
Qingdao University of Science and Technology, Box 70, Zhengzhou Road 53, Qingdao, Shandong, China. liujhye@sina.com.cn
Zhang
Yuanyuan
Y
Qiu
Long hui
Lh
Yang
Fengke
F
Ye
Lin
L
Xia
Yamu
Y
D004952:Esters / Q000737:chemistry / Q000378:metabolism; D006867:Hydrolases / Q000096:biosynthesis; D006868:Hydrolysis; D007660:Ketoprofen / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D011136:Polysorbates; D014250:Trichosporon / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D004952:Esters; D011136:Polysorbates; D007660:Ketoprofen; D006867:Hydrolases; D008049:Lipase