Cloning and characterisation of the immunophilin X-CypA in Xenopus laevis.
This paper reports the cloning of Xenopus laevis, cyclophilin A gene, X-CypA. This study is the first developmental and functional characterisation in vivo of cyclophilin A in a vertebrate. X-CypA belongs to the superfamily of the immunophilin/PPIase proteins that can bind the immunosuppressant drug Cyclosporin A. Sequence analysis showed that X-CypA is highly conserved during evolution. RT-PCR and in situ hybridisation analysis showed that X-CypA expression is regulated during development and its transcripts are found in three major expression domains: nervous system, sensory organs and pronephros. Over-expression of X-CypA in embryos, analysed by in situ hybridisation and RT-PCR, leads to an expansion and disorganisation of the neural crest domain.
Massé
Karine
K
Molecular Physiology, Department of Biological Sciences, Warwick University, Gibbet Hill Road, Coventry CV4 7AL, UK.
Bhamra
Surinder
S
Haldin
Caroline E
CE
Jones
Elizabeth A
EA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D021984:Cyclophilin A / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000002:abnormalities / Q000196:embryology* / Q000201:enzymology*; D010641:Phenotype; D016415:Sequence Alignment; D014982:Xenopus laevis
D018076:DNA, Complementary; D021984:Cyclophilin A