Methylation analysis of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma.
We investigated methylation status of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma (ATL). APC methylation was found in 15 of 31 (48%) primary samples, and 2 of 4 (50%) ATL cell lines. Methylation of the APC gene occurred more frequently in acute ATL (12/21) (57%) than chronic ATL (1/8) (13%) (P = 0.03). APC was not expressed in the APC-methylated ATL cell line ST1. Demethylation with 5-azacytidine treatment restored APC expression in the ST1 cell line. Our data show that hypermethylation of the APC gene is involved in the pathogenesis of ATL.
Yang
Yang
Y
Department of Medicine, Kochi Medical School, Okohcho, Nankoku, Kochi 783-8505, Japan.
Takeuchi
Seisho
S
Tsukasaki
Kunihiro
K
Yamada
Yasuaki
Y
Hata
Tomoko
T
Mori
Naoki
N
Fukushima
Atsuki
A
Seo
Hiromi
H
Koeffler
H Phillip
HP
Taguchi
Hirokuni
H
D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics*; D001374:Azacitidine / Q000494:pharmacology; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D018450:Disease Progression; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
D025601:Adenomatous Polyposis Coli Protein; D001374:Azacitidine