Probing macromolecular adsorbed layer structure and history dependence via the interfacial cavity function.
Adsorbed layers of proteins and other macromolecules often relax structurally more slowly than they form, rendering layer growth an out-of-equilibrium process. We show here how the interfacial cavity function, Phi (the average Boltzmann factor for a single probe molecule), may be determined, using kinetic data available from optical waveguide lightmode spectroscopy, and used as a continuous, in situ measure of history dependent adsorbed layer structure. The increase of Phi observed with residence time for fibronectin and lysozyme layers suggests post-adsorption clustering on a time scale longer than that predicted by a surface diffusion model.
Tie
Yanrong
Y
Department of Chemical Engineering, Yale University, New Haven, Connecticut 06520-8286, USA.
Ngankam
A Pascal
AP
Van Tassel
Paul R
PR
D000327:Adsorption; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry*; D008956:Models, Chemical; D010316:Particle Size; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods; D013499:Surface Properties
D046911:Macromolecular Substances