Inflammatory mediators and growth factors in the spinal cord of G93A SOD1 rats.
Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron disease. One mechanism involved in ALS pathology is neuroinflammation. Neuroinflammation is mediated by soluble pro-inflammatory molecules such as cytokines, prostaglandins and nitric oxide. Studies on transgenic mice demonstrated the expression of pro-inflammatory mediators in early stages of murine ALS. Recently a transgenic rat model became available. Since species differences in regard to cytokine expression have been reported in other disease models we set out to validate the neuroinflammatory hypothesis in the ALS-transgenic rat. We investigated the expression of inflammatory mediators and growth factors in the spinal cord by semi-quantitative RT-PCR. We found that several pro-inflammatory mediators are up-regulated at asymptomatic and end-stages, whereas VEGF, a neuroprotective factor was down-regulated.
Xie
Yiheng
Y
Department of Neurology, School of Medicine, University of Washington, Seattle, WA 98195-6465, USA.
Weydt
Patrick
P
Howland
David S
DS
Kliot
Michel
M
Möller
Thomas
T
D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D015152:Blotting, Northern / Q000379:methods; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013116:Spinal Cord / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000502:physiology*; D013997:Time Factors
D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; C109415:SOD1 G93A protein; D013482:Superoxide Dismutase