title: Clonal spread of IMP-1-producing Pseudomonas aeruginosa in two hospitals in Singapore.
abstract: Thirty-six isolates of carbapenem-resistant Pseudomonas aeruginosa were studied. Pulsed-field gel electrophoresis revealed the presence of two clones. One clone carried a bla(IMP-1) gene identical to that first described in Japan. The other clone carried a bla(IMP-1) variant containing four silent mutations. One isolate with a unique pulsed-field gel electrophoresis pattern contained bla(IMP-7).
authors.affiliation: Department of Pathology, Singapore General Hospital, 1 Hospital Dr., 169608 Singapore, Singapore. gptthk@sgh.com.sg
authors.forename: Tse Hsien
authors.initials: TH
authors.lastname: Koh
authors.forename: Grace Chee Yeng
authors.initials: GC
authors.lastname: Wang
authors.forename: Li-Hwei
authors.initials: LH
authors.lastname: Sng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D016521:Electrophoresis, Gel, Pulsed-Field; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012846:Singapore / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases