title: [IL-1beta and PGE2 production in whole blood and gingival fluid in women with periodontitis and preterm low birth weight].
abstract: METHODS
  The aim of the investigation was to evaluate of IL-1beta and PGE2 concentrations in gingival fluid, whole blood as well as IL-1beta, PGE2 production after Escherichia coli lipopolysaccharide stimulation in whole blood in women with preterm low birth weight (PLBW), as compared to the control group.
  
  
  METHODS
  A case-control study of 88 postpartum women aged 17 to 39 was performed. The case group consisted of 52 women with PLBW and the control group consisted of 36 women giving birth in time. Concentration of inflammatory mediators in gingival fluid, blood serum and IL-1beta, PGE2 production in whole blood after bacterial lipopolysaccharide stimulation were determined by means of immunoenzymatic method.
  
  
  CONCLUSIONS
  The levels of IL-1beta and PGE2 in gingival fluid were significantly higher in all PLBW mothers (also PLBW primiparous) than in the control group. In addition in the primiparous with PLBW group significantly higher PGE2 concentration in blood serum was found compared to the primiparous controls. There were no significant differences between women with PLBW and the controls together with a significantly higher production of IL-1beta and PGE2 in whole blood after LPS stimulation in women with periodontitis and gingivitis compared to subjects with healthy periodontium. Such findings suggest that inflammatory mediator synthesis is mainly result of specific cells exposition to bacterial products. Therefore it seems that more frequent occurrence of the phenotype of hyperactive cells that synthesise these mediators is not responsible for PLBW.
authors.affiliation: Katedra Periodontologii i Zakład Patologii, Jamy Ustnej AM we Wrocławiu. ; kierownik Zakladu: paradont@stom.am.wroc.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Konopka
authors.forename: Monika
authors.initials: M
authors.lastname: Rutkowska
authors.forename: Lidia
authors.initials: L
authors.lastname: Hirnle
authors.forename: Wacław
authors.initials: W
authors.lastname: Kopeć
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015232:Dinoprostone / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000150:complications / Q000378:metabolism; D006801:Humans; D007230:Infant, Low Birth Weight; D007231:Infant, Newborn; D007234:Infant, Premature; D018836:Inflammation Mediators / Q000378:metabolism; D007375:Interleukin-1 / Q000097:blood / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D015999:Multivariate Analysis; D010518:Periodontitis / Q000150:complications / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism / Q000382:microbiology; D011256:Pregnancy Outcome; D012307:Risk Factors
chemical_list: D018836:Inflammation Mediators; D007375:Interleukin-1; D008070:Lipopolysaccharides; D015232:Dinoprostone