Superheated water chromatography-nuclear magnetic resonance spectroscopy and mass spectrometry of vitamins.
The water-soluble vitamins, pyridoxine, riboflavin, and thiamine, were separated by reversed-phase liquid chromatography using hot or superheated water as the mobile phase and were detected using a range of detectors, including ultraviolet and fluorescence spectroscopy and mass spectrometry. By using deuterium oxide as the eluent, direct on-line nuclear magnetic resonance spectra could be obtained with minimal spectral interference from the mobile phase. Some of the compounds showed deuterium exchange of alkyl-protons when separated at high temperatures.
Chienthavorn
Orapin
O
Department of Chemistry, Loughborough University, Loughborough, Leics LE11 3TU, UK.
Smith
Roger M
RM
Saha
Shikha
S
Wilson
Ian D
ID
Wright
Brian
B
Taylor
Stephen D
SD
Lenz
Eva M
EM
D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D014815:Vitamins / Q000032:analysis*; D014867:Water / Q000032:analysis*
D014815:Vitamins; D014867:Water