title: Spectrophotometric determination of clozapine based on its oxidation with bromate in a micellar medium.
abstract: A simple and sensitive spectrophotometric method was developed for the determination of clozapine in its dosage forms. The method is based on the reaction of the drug with potassium bromate in a perchloric acid medium to produce an intense yellow colored species exhibiting a maximum absorption at 308 nm. Beer's law is obeyed for up to 12.0 microg ml(-1) with a correlation coefficient (n = 6) of 0.9998 and a detection limit (3S(b)) of 0.1 microg ml(-1). The molar absorptivity is 1.986 x 10(4) l mol(-1) cm(-1). The various experimental parameters affecting the development and stability of the colored oxidation product were carefully studied and optimized. The proposed method was successfully applied to the determination of clozapine in its dosage forms. The results obtained were in good agreement with those obtained using the B.P. official method.
authors.affiliation: Department of Chemistry, Faculty of Science, King Khalid University, Abha 9033, Saudi Arabia. aamohamd@hotmail.com
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: Mohamed
authors.forename: Sheikha M
authors.initials: SM
authors.lastname: Al-Ghannam
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis* / Q000737:chemistry; D001959:Bromates / Q000737:chemistry; D003024:Clozapine / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents; D008823:Micelles; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000737:chemistry; D011136:Polysorbates; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets
chemical_list: D014150:Antipsychotic Agents; D001959:Bromates; D007202:Indicators and Reagents; D008823:Micelles; D010472:Perchlorates; D011136:Polysorbates; D013607:Tablets; C019536:potassium bromate; D003024:Clozapine