title: Prenatal dietary docosahexaenoic acid supplementation in combination with protein restriction does not affect blood pressure in adult Wistar rats.
abstract: Recent findings indicate that prenatal protein restriction, which leads to elevated blood pressure in adult rats, results in decreased levels of docosahexaenoic acid (DHA) in neonatal rat brain. In light of the evidence of a relationship between dietary DHA and adult blood pressure, the purpose of this study was to ascertain whether prenatal dietary supplementation with DHA would prevent the development of hypertension associated with maternal protein restriction. Throughout gestation, female Wistar rats were fed isocaloric diets containing either 18% casein + 10% corn oil (CON; control), 9% casein + 10% corn oil (LP; low-protein) or 9% casein + 8.5% corn oil + 1.5% DHASCO (LP + 0.6% DHA). DHA increased levels of DHA in neonatal forebrain but there were no effects of LP. At 10 weeks there were no dietary effects on blood pressure measured on four consecutive days using tail-cuff plethysmography. There were also no significant effects measured at 30 weeks, using femoral artery catheterisation, despite adequate power to detect a 10 mm Hg difference. Trends in corticosterone measurements suggested higher stress reactivity in the LP group. These results do not provide strong support for the prenatal low protein model of hypertension and a relation with dietary DHA.
authors.affiliation: Department of Health Studies and Gerontology, University of Waterloo, 200 University Avenue W, Waterloo, Ont., Canada N2L 3G1.
authors.forename: D A
authors.initials: DA
authors.lastname: Martin
authors.forename: D
authors.initials: D
authors.lastname: McCutcheon
authors.forename: P E
authors.initials: PE
authors.lastname: Wainwright
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001794:Blood Pressure; D002364:Caseins / Q000008:administration & dosage; D003314:Corn Oil / Q000008:administration & dosage; D003345:Corticosterone / Q000097:blood; D018753:Diet, Protein-Restricted; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000008:administration & dosage*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006973:Hypertension / Q000209:etiology / Q000517:prevention & control; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D011297:Prenatal Exposure Delayed Effects; D016548:Prosencephalon / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014886:Weaning; D015430:Weight Gain
chemical_list: D002364:Caseins; D005227:Fatty Acids; D004281:Docosahexaenoic Acids; D003314:Corn Oil; D003345:Corticosterone