title: Application of difference NOE-pumping NMR technique and cold-spray ionization mass spectrometry to identify a ligand binding with a protein receptor.
abstract: A difference diffusion-based NMR technique and cold-spray ionization mass spectrometry were employed as a solution-based approach for identifying a ligand binding with a protein receptor. The difference diffusion-based NMR technique, called difference NOE-pumping, can directly detect the ligand interacting with a protein receptor. This technique uses a simple pulse sequence and the diffusion filter can easily be optimized. The cold-spray ionization mass spectrometry (CSI-MS), a variant of electrospray ionization mass spectrometry (ESI-MS) operating at low temperature, has been applied to detect the ligand-receptor complex. The efficiency of these techniques for identifying binding ligands is demonstrated with the human serum albumin (HSA)-drug system.
authors.affiliation: Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba 263-8522, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Seki
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Sei
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Shikii
authors.forename: Sakurako
authors.initials: S
authors.lastname: Shimotakahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Utsumi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Tashiro
mesh_terms: D005665:Furosemide / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D020156:Salicylic Acid / Q000032:analysis / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature; D014859:Warfarin / Q000032:analysis / Q000737:chemistry
chemical_list: D008024:Ligands; D012709:Serum Albumin; D014859:Warfarin; D005665:Furosemide; D020156:Salicylic Acid