High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging.
We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.
Requejo-Isidro
J
J
Department of Physics, Division of Investigative Sciences and Department of Bioengineering, Imperial College London, London, UK. j.requejo@imperial.ac.uk
McGinty
J
J
Munro
I
I
Elson
D S
DS
Galletly
N P
NP
Lever
M J
MJ
Neil
M A A
MA
Stamp
G W H
GW
French
P M W
PM
Kellett
P A
PA
Hares
J D
JD
Dymoke-Bradshaw
A K L
AK
D000465:Algorithms; D019723:Endoscopes; D004724:Endoscopy / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014741:Video Recording / Q000295:instrumentation* / Q000379:methods