Dexamethasone induces different wheel running activity than corticosterone through vasopressin release from the suprachiasmatic nucleus.
During the analysis of wheel running activity, we found that corticosterone (1 mg/100 g BW) injection decreased wheel activity, while dexamethasone (0.1 mg/100 g) increased the activity. To clarify the functional differences between corticosterone and dexamethasone, we measured Arg-vasopressin (AVP) release from the suprachiasmatic nucleus (SCN) slice culture in vitro and AVP coding mRNA in the SCN in vivo. The corticosterone (0.2 and 2 microg/ml, final concentration in medium) decreased the AVP release, while it increased by dexamethasone (0.2 and 2 microg/ml). An AVP mRNA in the SCN was decreased by both corticosterone (1 mg/100 g) and dexamethasone (0.1 mg/100 g). The differences in wheel activity by corticosterone and dexamethasone are discussed from the changes of AVP in the SCN.
Isobe
Yoshiaki
Y
Department of Neuro-physiology and Brain Sciences, Nagoya City University, Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan. yisobe@med.nagoya-cu.ac.jp
Torii
Takako
T
Kawaguchi
Takatsune
T
Nishino
Hitoo
H
D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001522:Behavior, Animal; D003345:Corticosterone / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007124:Immunoenzyme Techniques / Q000379:methods; D009043:Motor Activity / Q000187:drug effects*; D009924:Organ Culture Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012420:Running; D013493:Suprachiasmatic Nucleus / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D014667:Vasopressins; D003907:Dexamethasone; D003345:Corticosterone