title: Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin.
abstract: The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Guérin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA.
authors.affiliation: Department of Pathophysiology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jing
authors.forename: Sun
authors.initials: S
authors.lastname: Shuhan
authors.forename: Hu
authors.initials: H
authors.lastname: Zhenlin
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fengjuan
authors.forename: Ling
authors.initials: L
authors.lastname: Yiling
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004269:DNA, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005612:Freeze Drying; D016680:Genome, Bacterial; D009163:Mycobacterium bovis / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D004269:DNA, Bacterial