title: Preparation and characterization of long methacrylate monolithic column for capillary liquid chromatography.
abstract: Long methacrylate monolithic columns (100 cm x 320 microm i.d.) were prepared from silanized fused-silica capillaries of 320 microm i.d. by in situ copolymerization of butyl methacrylate (BMA) with ethylene dimethacrylate (EDMA) in the presence of a suitable porogen. The separation performance and selectivity of the column were evaluated and compared with a 25 cm x 320 microm i.d. column prepared in the same way by capillary high-performance liquid chromatography (micro-HPLC). The results showed that the 1 m long monolithic column can generate 33 x 10(3) plate number and exhibited good permeability, higher sample loadability, and separation capability.
authors.affiliation: Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shu
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Guan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010539:Permeability
chemical_list: D008689:Methacrylates