title: Osteogenic potential of human adipose tissue-derived stromal cells as an alternative stem cell source.
abstract: Adult bone marrow contains mesenchymal stem cells (bone marrow-derived mesenchymal stem cells; BMSCs) which contribute to the generation of mesenchymal tissue such as bone, cartilage, muscle and adipose. However, using bone marrow as a source of stem cells has the limitation of a low cell number. An alternate source of adult stem cells that could be obtained in large quantities, under local anesthesia, with minimal discomfort would be advantageous. Human adipose tissue obtained by liposuction was processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). In this study, we compared the osteogenic differentiation of ATSCs with that of BMSCs. Both cell types were cultured in atelocollagen honeycomb-shaped scaffolds with a membrane seal (ACHMS scaffold) for three-dimensional culturing in a specific osteogenic induction medium. Optimal osteogenic differentiation in both cell types, as determined by alkaline phosphatase cytochemistry, secretion of osteocalcin, mineral (calcium phosphate) deposition and scanning electron microscopy, was obtained with the same three-dimensional culture. Furthermore, osteoblastic lining in vivo was examined using ATSC-seeded or BMSC-seeded scaffolds in nude mice. The present results show that ATSCs have a similar ability to differentiate into osteoblasts to that of BMSCs.
authors.affiliation: Division of Biomedical Engineering, Research Institute, National Defense Medical College, Saitama, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.forename: Kazunori
authors.initials: K
authors.lastname: Masuoka
authors.forename: Miya
authors.initials: M
authors.lastname: Ishihara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Takemi
authors.initials: T
authors.lastname: Matsui
authors.forename: Bonpei
authors.initials: B
authors.lastname: Takase
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Fujikawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology
chemical_list: D015675:Osteocalcin; D000469:Alkaline Phosphatase