title: On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin.
abstract: Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on-line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on-line cation exchange in order to prevent massive adducting in the ESI interface.
authors.affiliation: Department of Spectroscopy, LEO Pharma A/S, 2750 Ballerup, Denmark. jens.henriksen@leo-pharma.com
authors.forename: Jens
authors.initials: J
authors.lastname: Henriksen
authors.forename: Lene Hoffmeyer
authors.initials: LH
authors.lastname: Ringborg
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorrf
mesh_terms: D000107:Acetylation; D002412:Cations / Q000737:chemistry; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005343:Fibrinolytic Agents / Q000032:analysis* / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000032:analysis* / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000032:analysis / Q000737:chemistry; D000078222:Tinzaparin
chemical_list: D002412:Cations; D005343:Fibrinolytic Agents; D006495:Heparin, Low-Molecular-Weight; D009844:Oligosaccharides; D013431:Sulfates; D000078222:Tinzaparin