Promoter analysis of cgl2, a galectin encoding gene transcribed during fruiting body formation in Coprinopsis cinerea (Coprinus cinereus).
In the homobasidiomycete Coprinopsis cinerea, expression of the two fruiting body-specific galectins, CGL1 and CGL2, is controlled by nutrients, light and darkness and the A mating type genes. In this study, we analyzed the promoter of the cgl2 gene by measuring transcript levels by quantitative real-time PCR and show that regulation of CGL2 expression occurs at the transcriptional level. A minimal promoter sufficient to confer regulated expression of a heterologous reporter gene and comprising 627 base pairs from the start codon was defined. On the minimal promoter we identified a 120 bp sequence mediating induction of the cgl2 gene in constant darkness. Along with direct repeats (TGGAAG/TGGAAG/GGAA), the sequence contains a CRE consensus site (cAMP-responsive element, TGCGTCA) suggesting the involvement of cAMP signaling in cgl2 activation. No specific elements responsible for light repression and mating type regulation were found in the promoter.
Bertossa
Rinaldo C
RC
Institute of Microbiology, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland.
Kües
Ursula
U
Aebi
Markus
M
Künzler
Markus
M
D000222:Adaptation, Physiological; D019677:Artificial Gene Fusion; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003302:Coprinus / Q000235:genetics*; D003624:Darkness; D005656:Fungal Proteins / Q000235:genetics*; D037501:Galectin 2; D037161:Galectins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D014158:Transcription, Genetic
C103751:CGL2 protein, Coprinus cinereus; D005656:Fungal Proteins; D037501:Galectin 2; D037161:Galectins; D012331:RNA, Fungal; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins