[A new method for phylogenic analysis].
With ESTs from porcine fatty tissue and cDNA sequences from human, bovine and mouse in non-reduncdant database and dbEST in GenBank,we sampled cDNA sequences of 70 function-known genes in four species on the base of randomly sampling method, analyzed the mutation pattern of 70 x 150 bp linking sequences between them, and established an integrated phylogenic analysis method. The results showed that 391 single bases mutations were found in 70 x 150 bp linking sequences alignment among four species. The number of mutation bases between them were greatly exceeded the 1/1000 predicted in the human genome analysis. C/T(T/C) and A/G (G/A) transitions were the major types of single base mutation. The genetic relationship between pig and bovine who are both Artiodactylous is the nearest, the next is human, and the farthest is mouse. The differentiation sequence taken place in four species from the same ancestor is that mouse is the earliest one, and the latter human, and pig and bovine are the latest.
Zhao
Zhi-Hui
ZH
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China. zh65zh@sina.com.cn
Fan
Bao-Liang
BL
Deng
Xue-Mei
XM
Hu
Xiao-Xiang
XX
Xu
Jian-Hua
JH
Li
Ning
N
Wu
Chang-Xin
CX
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D020224:Expressed Sequence Tags; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine