Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain.
Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.
Aranaz
Alicia
A
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. alaranaz@vet.ucm.es
Romero
Beatriz
B
Montero
Natalia
N
Alvarez
Julio
J
Bezos
Javier
J
de Juan
Lucía
L
Mateos
Ana
A
Domínguez
Lucas
L
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007753:Laboratories; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D009841:Oligonucleotides / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D009841:Oligonucleotides