[The anti-respiratory syncytial virus (RSV) effect of Radix Glycyrrhizae in vitro].
OBJECTIVE
To develop safe and effective anti-RSV new medicine from Radix Glycyrrhizae.


METHODS
The anti-RSV effect of Radix Glycyrrhizae in Hela cell culture was observed by means of the inhibition of cytopathic effect.


RESULTS
In Hela cell culture, Radix Glycyrrhizae was found to be a inhibitor of RSV in a concentration-dependent manner. The median toxic concentration (TC50) of Radix Glycyrrhizae was 3.43 g/L, the median effective concentration (EC50) of Radix glycyrrhizae against replication of the Long strain of RSV in Hela cells were 0.2535 g/L, the selectivity index (TI = TC50/EC50) is 13.53. In time of addition experiment, Radix Glycyrrhizae inhibited the effect of RSV in Hela cells when it was added at 0 h, 2 h, 4 h, 6 h, 8 h after virus infection.


CONCLUSIONS
In Hela cell culture, Radix Glycyrrhizae was found to be a inhibitor of RSV, there are many ways in the mechanisms.
Dong
Yanmei
Y
College of Public Health, Harbin Medical University, Harbin.
Li
Hongyuan
H
Yao
Zhenjiang
Z
Tian
Wenjing
W
Han
Zhigang
Z
Qiu
Haiyan
H
Piao
Yingai
Y
D000998:Antiviral Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D012254:Ribavirin / Q000494:pharmacology; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012254:Ribavirin