A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine.
Deuterium solid-state NMR spectroscopy was used to qualitatively study the effects of both 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphatidylcholine (PLiPC) and cholesterol on magnetically aligned phospholipid bilayers (bicelles) as a function of temperature utilizing the chain-perdeuterated probe 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC-d54) in DMPC/dihexanoylPC (DHPC) phospholipid bilayers. The results demonstrate that polyunsaturated PC and cholesterol were successfully incorporated into DMPC/DHPC phospholipid bilayers, leading to a bicelle that will be useful for investigations of eukaryotic membrane protein-lipid interactions. The data indicate that polyunsaturated PC increases membrane fluidity and decreases the minimum magnetic alignment temperature for DMPC/DHPC bicelles. Conversely, the introduction of cholesterol into aligned DMPC/DHPC bilayers decreases fluidity in the membrane and increases the minimum temperature necessary to magnetically align the phospholipid bilayers. Finally, the addition of Tm3+ to magnetically aligned DMPC/DMPC-d54/PLiPC/DHPC bilayers doubles the quadrupolar splittings, indicating that this unique bicelle system can be aligned with the bilayer normal parallel to the static magnetic field.
Minto
Robert E
RE
Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Adhikari
Prem R
PR
Lorigan
Gary A
GA
D040761:Biomimetic Materials / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D003903:Deuterium; D004574:Electromagnetic Fields; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000528:radiation effects; D008081:Liposomes / Q000737:chemistry* / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008560:Membrane Fluidity; D008823:Micelles; D008968:Molecular Conformation; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry*; D013696:Temperature
D005231:Fatty Acids, Unsaturated; D008051:Lipid Bilayers; D008081:Liposomes; D008823:Micelles; D010713:Phosphatidylcholines; C055402:1-palmitoyl-2-linoleoylphosphatidylcholine; D002784:Cholesterol; D003903:Deuterium