title: The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of Lymnaea columella, L. viatrix, and L. diaphana (Mollusca: Lymnaeidae).
abstract: The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. In the present study, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques targeted to the first and second internal transcribed spacers (ITS1 and ITS2) rDNA and to the mitochondrial 16S ribosomal gene (16S rDNAmt) were used to differentiate the species Lymnaea columella, L. viatrix, and L. diaphana from some localities of Brazil, Argentina, and Uruguay as well as to verify whether the molecular results corroborates the classical morphological method.PCR-RFLP analysis of the ITS1, ITS2, and 16S using 12 restriction enzymes revealed characteristic patterns for L. columella and L. diaphana which were concordant with the classical morphology. On the other hand, for L. viatrix populations a number of 1 to 6 profiles were generated while morphology provided the species pattern results.
authors.affiliation: Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brazil. omar@cpqrr.fiocruz.br
authors.forename: Omar S
authors.initials: OS
authors.lastname: Carvalho
authors.forename: Paula C M
authors.initials: PC
authors.lastname: Cardoso
authors.forename: Pollanah M
authors.initials: PM
authors.lastname: Lira
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rumi
authors.forename: Andrea
authors.initials: A
authors.lastname: Roche
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Berne
authors.forename: Gertrud
authors.initials: G
authors.lastname: Müller
authors.forename: Roberta L
authors.initials: RL
authors.lastname: Caldeira
mesh_terms: D000818:Animals; D001938:Brazil; D018509:DNA, Helminth / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005819:Genetic Markers; D008195:Lymnaea / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers