title: Measles virus genotype circulation in Spain after implementation of the national measles elimination plan 2001-2003.
abstract: Molecular characterization of measles virus is important for disease surveillance and for monitoring elimination of the virus throughout the world. Furthermore, knowledge of genotype distribution in as many countries as possible, is useful for tracing the origin of a strain, especially in countries without endemic measles disease, where most cases are imported. Data on genotypes circulating in Spain from 1970 to 1997 showed the prevalence of genotypes C1, C2, and D6, with subsequent replacement of each other. After the establishment of the Spanish Measles Elimination Plan, genotyping with a new retrotranscriptase polymerase chain reaction (RT-PCR) was undertaken directly on 92 specimens, corresponding to 90 patients, which were positive for measles by a different diagnostic RT-PCR. Genotypes B3, D4, D8, A, C2, H1, and D7 were found in different autonomous communities (Madrid, Balearic Islands, Valencia Community, Extremadura, Andalusia, Canary Islands and Murcia) between 2001 and 2003 with none of these genotypes being prevalent. After the introduction of the vaccine in 1978, the incidence of the disease decreased from 150,000 cases in 1977 to 64 in 2002. This could be the reason for the change observed in the pattern of measles genotype circulation, since this pattern was reported in countries at an advanced stage of eradication of measles. This report considers that Spain is on the way to eradicating measles.
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera de Majadahonda-Pozuelo s/n, 28220 Majadahonda, Madrid, Spain. mmosquera@isciii.es
authors.forename: María Mar
authors.initials: MM
authors.lastname: Mosquera
authors.forename: Fernando de
authors.initials: Fd
authors.lastname: Ory
authors.forename: Juan Emilio
authors.initials: JE
authors.lastname: Echevarría
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D005838:Genotype; D006801:Humans; D017589:Immunization Programs; D008457:Measles / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D008458:Measles Vaccine; D008459:Measles virus / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013030:Spain / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D008458:Measles Vaccine; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus