Separation of flavonoids from Aleurites moluccana leaves using chitosan modified with heptaldehyde.
Heptaldehyde-modified chitosan (heptyl-chitosan, CH-Hp) was investigated as adsorbent for chromatograhic separation of the flavonoids from A. moluccana. The amount of 2"-O-rhamnosylswertisin isolated (30.0 mg) was approx. twice as high as swertisin (17.5 mg). The improved surface hydrophobicity effected by the heptyl groups promoted the separation of flavonoids. From the results obtained, CH-Hp seems to be more suitable for separation of glycosylated flavonoids than other flavonoids. Thus, modified chitosan described here can be used for hydrophobic interaction chromatography as sucessfully illustrated with flavonoids.
Morsch
Michele
M
Núcleo de Investigações Químico-Farmacêuticas, Curso de Farmácia/CCS, Universidade do Vale do Itajaí, CEP 88.302-202, Itajaí, SC, Brasil.
Girardi
Leury G J
LG
Cechinel-Filho
Valdir
V
Meyre-Silva
Christiane
C
Rodrigues
Clóvis Antonio
CA
D000107:Acetylation; D000447:Aldehydes; D031284:Aleurites / Q000737:chemistry*; D002240:Carbohydrate Sequence; D048271:Chitosan; D002845:Chromatography / Q000379:methods; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods
D000447:Aldehydes; D005419:Flavonoids; D007202:Indicators and Reagents; D009844:Oligosaccharides; D048271:Chitosan