Determination of light chain restriction in fine-needle aspiration-type preparations of B-cell lymphomas by mRNA in situ hybridization.
The purpose of this study was to investigate whether B-cell clonality could be demonstrated in fine-needle aspiration (FNA)-type preparations by using automated and manual in situ hybridization (ISH) systems. FNA-like preparations were made from 10 cases of B-cell lymphoma and 5 cases of reactive lymphoid hyperplasia. Kappa/lambda expression was determined using an automated mRNA ISH assay or a manual ISH system. Other variables tested included type and length of fixation, protease digestion, and time in chromogen. Clonality data were corroborated by either flow cytometry or tissue-based analysis. Optimal conditions required formalin fixation, strong protease digestion, and prolonged hybridization and chromogen times; under these conditions, monoclonality was demonstrated by in situ in 8 of 10 cases. Each of the five cases of reactive lymphoid hyperplasia showed polyclonal light chain expression by automated mRNA ISH. In situ kappa/lambda mRNA analysis of FNA-type specimens allows direct determination of monoclonality in cytologic preparations.
Lee
Lawrence H
LH
Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio 43210, USA.
Cioc
Adina
A
Nuovo
Gerard J
GJ
D000911:Antibodies, Monoclonal / Q000235:genetics; D044963:Biopsy, Fine-Needle; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods*; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D014066:Palatine Tonsil / Q000276:immunology / Q000473:pathology; D019310:Pseudolymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D012333:RNA, Messenger