title: Lack of association of matrix metalloproteinase 3 (MMP3) genotypes with ankylosing spondylitis susceptibility and severity.
abstract: OBJECTIVE
  To study the linkage and association of ankylosing spondylitis (AS) with genotypes for matrix metalloproteinase 3 (MMP3), a gene located at chromosome 11q22.3 and lying within the 101-124 cM region observed in a recent genome-wide scan as a region associated with AS susceptibility.
  
  
  METHODS
  MMP3 genotypes were examined in 229 pedigrees with AS, 131 sporadic AS cases and 87 Caucasian controls. Eight single-nucleotide polymorphisms (SNPs) were selected and genotyped using Taqman. Non-parametric linkage (NPL) analysis was conducted between the eight MMP3 SNPs and AS using the NPL-all statistic and two-point parametric linkage analysis using GeneHunter Plus. Unrelated AS cases and controls were compared using chi2 statistics, and family-based controls using the transmission disequilibrium test and pedigree disequilibrium test.
  
  
  RESULTS
  None of the eight MMP3 SNPs were significantly associated with AS, either using the 131 sporadic cases alone or in analyses which combined these cases with the 226 unrelated affected AS patients derived from the pedigrees. Analysis of linkage disequilibrium (LD) demonstrated that seven of the eight SNPs studied were in strong LD except for rs626750, which is about 6 kb upstream of the 5' end of the gene. No significant linkage was observed using NPL and LODs in the families. No association was seen of any of the MMP3 SNPs with disease severity (defined by patient functioning), as measured either by the Bath Ankylosing Spondylitis Functional Index or the modified Health Assessment Questionnaire.
  
  
  CONCLUSIONS
  These data suggest that MMP3 genotypes are not involved in AS susceptibility or severity.
authors.affiliation: University of Cincinnati, OH, USA.
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.forename: M
authors.initials: M
authors.lastname: Weisman
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Ward
authors.forename: J
authors.initials: J
authors.lastname: Luo
authors.forename: J
authors.initials: J
authors.lastname: Bruckel
authors.forename: R
authors.initials: R
authors.lastname: Inman
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.forename: H R
authors.initials: HR
authors.lastname: Schumacher
authors.forename: W P
authors.initials: WP
authors.lastname: Maksymowych
authors.forename: M
authors.initials: M
authors.lastname: Mahowald
authors.forename: T
authors.initials: T
authors.lastname: Martin
authors.forename: J T
authors.initials: JT
authors.lastname: Rosenbaum
authors.forename: D T Y
authors.initials: DT
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Stone
authors.forename: J
authors.initials: J
authors.lastname: Watson
authors.forename: E
authors.initials: E
authors.lastname: Dickman
authors.forename: J
authors.initials: J
authors.lastname: Davis
authors.forename: J D
authors.initials: JD
authors.lastname: Reveille
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D013167:Spondylitis, Ankylosing / Q000201:enzymology* / Q000235:genetics*
chemical_list: D019278:Matrix Metalloproteinase 3