Use of monoclonal antibodies for analyses of Coxiella burnetii major antigens.
Monoclonal antibodies (MAbs) to major antigens of Coxiella burnetii were produced. Some of the MAbs to a 62-kDa protein antigen, peptidoglycan protein complex and lipopolysaccharide (LPS) O-chains reacted with other bacteria whereas none of the MAbs to outer membrane proteins and LPS outer-core did. The LPS outer-core and OMPs may be useful antigens for specifically detecting antibodies to C. burnetii.
Hotta
Akitoyo
A
Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan.
Zhang
Guo Quan
GQ
Andoh
Masako
M
Yamaguchi
Tsuyoshi
T
Fukushi
Hideto
H
Hirai
Katsuya
K
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D016997:Coxiella burnetii / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D008070:Lipopolysaccharides / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D008565:Membrane Proteins