title: Immune system-mediated endothelial damage is associated with NO and antioxidant system disorders.
abstract: Two distinct systems of different origin are involved in the pathogenesis of both infectious and immunological vasculitis syndrome: nitric oxide (NO) from endothelial cells and granulocyte NADPH oxidase. In this study, in 31 children with immune system dysfunction, NO, NO synthase (NOS) and antioxidant enzyme activities [catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx)], as well as immunological parameters, were investigated. On the basis of the clinical findings, all children were divided into three groups: group I, 8 children clinically showing macular skin manifestations; group II, 11 children with maculo-papulous changes; and group III, 12 children with clinical findings of papulous changes. Plasma NO values in groups II and III were significantly elevated (79.14+/-30.13 and 65.32+/-6.70 micromol/l), compared to the control group (41.24+/-3.65 micromol/l), while group I showed statistically lower values (32.38+/-3.37 micromol/l). In children with the highest level of NO (group II) NOS activity was two-fold higher (1.77+/-0.59 nmol/ml/min; p<0.01) than in controls (0.98+/-0.23 nmol/ml/min). Catalase activity showed a significant increase and SOD activity a significant decrease in all experimental groups, while GPx was not significantly changed. The results show that immune system dysfunction manifested as vasculitis is associated with significant disturbances in the NO system and free radicals scavengers.
authors.affiliation: Center of Medical Biochemistry, Clinical Center Nis, Nis, Serbia and Montenegro.
authors.forename: Vidosava B
authors.initials: VB
authors.lastname: Djordjević
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stanković
authors.forename: Vladan
authors.initials: V
authors.lastname: Cosić
authors.forename: Lilika
authors.initials: L
authors.lastname: Zvezdanović
authors.forename: Borisav
authors.initials: B
authors.lastname: Kamenov
authors.forename: Desanka
authors.initials: D
authors.lastname: Tasić-Dimov
authors.forename: Ivana
authors.initials: I
authors.lastname: Stojanović
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000097:blood / Q000378:metabolism; D002648:Child; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D006801:Humans; D007107:Immune System / Q000378:metabolism*; D008659:Metabolic Diseases / Q000378:metabolism* / Q000473:pathology; D019255:NADPH Oxidases / Q000097:blood / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009569:Nitric Oxide; D002374:Catalase; D005979:Glutathione Peroxidase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases