Strategies for shotgun identification of post-translational modifications by mass spectrometry.
The global identification of post-translationally modified proteins is a difficult challenge that is currently being addressed by many researchers in the field of mass spectrometry (MS)-based proteomics. The ability to identify thousands of proteins by shotgun-based strategies has made the mere idea of a global analysis of a particular protein modification seem reasonable. There has been much progress in the development of methods that make use of shotgun-based protein identification in the analysis of a wide variety of protein modifications, some of which will be discussed here.
Cantin
Greg T
GT
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road SR11, La Jolla, CA 92037, USA.
Yates
John R
JR
D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational; D040901:Proteomics