title: Association of VEGF genotype with mRNA level in colorectal adenocarcinomas.
abstract: The mRNA expression of vascular endothelial growth factor (VEGF) was evaluated in colorectal adenocarcinomas and adjacent noncancerous colorectal tissues in 18 Japanese patients. The expression was confirmed to be up-regulated in the colorectal adenocarcinomas, when compared with the noncancerous tissues. Twelve genotypes of VEGF: six positions in the promoter region, two in the 5'UTR, and four in the 3'UTR, and their association with the expression of VEGF mRNA were evaluated. While G-1877A, T-1455C, G-1154A, C702T, and G1612A were not detected, C-2578A, T-1498C, G-1190A, C-634G, C-7T, C936T, and C1451T were found at allele frequencies of 4/36, 15/36, 15/36, 20/36, 8/36, 6/36, and 6/36, respectively, suggesting that C-2578A, G-1154A, and G1612A were associated with a decreased risk for colorectal adenocarcinoma. T-1498C (G-1190A) and C-7T were found to be associated with higher levels of VEGF mRNA, and may be a risk factor for the development of liver metastasis and/or prognosis of colorectal adenocarcinoma.
authors.affiliation: Department of Hospital Pharmacy, School of Medicine, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Yamamori
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaeda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Noboru
authors.initials: N
authors.lastname: Okamura
authors.forename: Takao
authors.initials: T
authors.lastname: Tamura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Aoyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamigaki
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ohno
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Akinobu
authors.initials: A
authors.lastname: Gotoh
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Matsuo
authors.forename: Masato
authors.initials: M
authors.lastname: Kasuga
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism; D004252:DNA Mutational Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A