Simultaneous determination of 2-phenylbenzotriazole-type mutagens, PBTA-1 through -8, in river water by liquid chromatography-tandem mass spectrometry.
We describe a method for the simultaneous determination of eight kinds of phenylbenzotriazole-type mutagens (PBTA-1, -2, -3, -4, -5, -6, -7 and -8) in river water based on liquid chromatography-tandem mass spectrometry (LC/MS/MS). The application of dopant-assisted atmospheric pressure photoionization (APPI) for the detection of the PBTAs was studied. The APPI technique provided higher PBTA signal intensities than those obtained with an electrospray ionization (ESI) source, and the APPI method was used for the determination of the PBTAs. A solid-phase extraction procedure was used for the extractions of PBTA-1 through -8 from river water. The procedure was rapid and the relative standard deviations were below 15%. The detection limits of PBTA-1 through -8 in river water using the proposed method were found to range from 0.04 to 0.5 ng L(-1) and PBTAs were successfully detected in river water at sub-ng L(-1) levels.
Moriwaki
Hiroshi
H
Osaka City Institute of Public Health & Environmental Sciences, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. hiroshi.moriwaki@iphes.city.osaka.jp
Harino
Hiroya
H
Yoshikura
Taro
T
Ohe
Takeshi
T
Nukaya
Haruo
H
Terao
Yoshiyasu
Y
Sawanishi
Hiroyuki
H
Wakabayashi
Keiji
K
Miyakoda
Hidekazu
H
Alary
Jean-François
JF
D002853:Chromatography, Liquid; D004784:Environmental Monitoring / Q000379:methods; D007564:Japan; D013058:Mass Spectrometry / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D045483:Rivers / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014230:Triazoles / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
C115463:2-(2-(acetylamino)-4-(N-(2-cyanoethyl)ethylamino)-5-methoxyphenyl)-5-amino-7-bromo-4-chloro-2H-benzotriazole; D009153:Mutagens; D014230:Triazoles; D014874:Water Pollutants, Chemical