title: Lack of ultrastructural detrusor changes following endoscopic injection of botulinum toxin type a in overactive neurogenic bladder.
abstract: BACKGROUND
  Endoscopical injections of Botulinum toxin type A into the detrusor muscle are gaining clinical acceptance in the treatment of neurogenic detrusor overactivity. Structural effects of Botulinum toxin type A are only known from studies on striated muscles, where a widespread nerve sprouting occurs temporarily. The aim of this study was to evaluate the ultrastructural effects of Botulinum toxin type A injections on the human detrusor.
  
  
  METHODS
  30 detrusor biopsies were obtained from 24 patients with neurogenic detrusor overactivity. Patients were divided into two groups: Group I included 13 biopsies from patients before the first Botulinum toxin type A injection. Group II included 6 biopsies from patients within 3 months after the first injection and 11 biopsies at the time of decreasing efficacy of Botulinum toxin type A. The biopsies were processed by standard procedure for detailed electron microscopic study and evaluated by 2 examiners without prior knowledge of clinical/urodynamic data.
  
  
  RESULTS
  No statistically significant detrusor changes have been found concerning muscle cell fascicle structure (p = 0.445), width of intercellular space (p = 0.482) and number/kind of muscle cell junctions (p = 0.443). A median of 70% of intrinsic axon terminals presented with signs of degeneration in group I, a median of 66% in group II (p = 0.840). Out of 309 evaluated axon terminals in both groups, 1 sprouting axon was found in group I, 3 sprouting axons in group II (p = 0.864). Specimen from group I and group II showed only limited collagen deposits within the detrusor. No changes in the ultrastructure of the detrusor have been observed in those biopsies obtained before and after the Botulinum toxin type A injection of the same patient.
  
  
  CONCLUSIONS
  This study verifies our earlier report of severe intrinsic axon degeneration in the detrusor of patients with neurogenic detrusor overactivity. It also shows nearly no structural differences of the detrusor before and after Botulinum toxin type A injections. Contrary to reports of striated muscle, axonal sprouting within the detrusor was very limited after Botulinum toxin type A injections indicating pathophysiologically different reactions to the toxin either between striated muscle and smooth muscle or between different treated diseases.
authors.affiliation: Department of Urology, University of Bonn, Bonn, Germany. Axel_Haferkamp@med.uni-heidelberg.de
authors.forename: A
authors.initials: A
authors.lastname: Haferkamp
authors.forename: B
authors.initials: B
authors.lastname: Schurch
authors.forename: A
authors.initials: A
authors.lastname: Reitz
authors.forename: U
authors.initials: U
authors.lastname: Krengel
authors.forename: J
authors.initials: J
authors.lastname: Grosse
authors.forename: G
authors.initials: G
authors.lastname: Kramer
authors.forename: S
authors.initials: S
authors.lastname: Schumacher
authors.forename: P J
authors.initials: PJ
authors.lastname: Bastian
authors.forename: R
authors.initials: R
authors.lastname: Büttner
authors.forename: S C
authors.initials: SC
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Stöhrer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage*; D003558:Cystoscopy; D005260:Female; D006801:Humans; D015552:Injections, Intralesional / Q000379:methods; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects* / Q000648:ultrastructure*; D009465:Neuromuscular Agents / Q000008:administration & dosage*; D001743:Urinary Bladder / Q000187:drug effects* / Q000648:ultrastructure*; D001750:Urinary Bladder, Neurogenic / Q000188:drug therapy*
chemical_list: D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A