title: Effect of microcystin-LR on protein phosphatase activity and glycogen content in isolated hepatocytes of fed and fasted juvenile goldfish Carassius auratus L.
abstract: Toxic effects of microcystin-LR were studied in hepatocytes isolated from fed and fasted juvenile goldfish Carassius auratus (30 g body weight). The hepatocytes were incubated with 10 microgMC-LR l(-1) during 4 h. MC-LR induces no effect in terms of cell number and viability. The toxin accumulation pattern was different in fed and fasted treatments. MC-LR accumulated more rapidly in 'fasted' cells where the highest concentration was observed by 1 h of exposure. It was delayed to 4 h in the 'fed' cells. MC-LR accumulation induced a severe decrease in hepatic protein phosphatase activity in both treatments. It was almost totally inhibited in both treatments during the first hour of exposure. The glycogen content was significantly reduced after 2 h of exposure in the fasting treatments, but not in the feeding one.
authors.affiliation: Unité de Recherche en Biologie des Organismes, Facultés Universitaires Notre-Dame de la Paix, rue de Bruxelles, 61, B-5000 Namur, Belgium. christelle.malbrouck@fundp.ac.be
authors.forename: Christelle
authors.initials: C
authors.lastname: Malbrouck
authors.forename: Gérard
authors.initials: G
authors.lastname: Trausch
authors.forename: Pierre
authors.initials: P
authors.lastname: Devos
authors.forename: Patrick
authors.initials: P
authors.lastname: Kestemont
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D005215:Fasting; D006054:Goldfish / Q000502:physiology*; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D066298:In Vitro Techniques; D008112:Liver Glycogen / Q000378:metabolism*; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000633:toxicity*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D008112:Liver Glycogen; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; D010749:Phosphoprotein Phosphatases; C057862:cyanoginosin LR