DNA and low titer, helper-free, recombinant AAV prime-boost vaccination for cytomegalovirus induces an immune response to CMV-pp65 and CMV-IE1 in transgenic HLA A*0201 mice.
A prime-boost immunization regimen allowed the use of low titer, helper-free rAAV-pp65mII and rAAV-IE1 virus to elicit specific humoral and cellular responses to two important cytomegalovirus (CMV) antigens: the immediate-early 1 (IE-1) and pp65 proteins. Simultaneous immunization of both CMV proteins, using DNA vaccine priming followed by rAAV boost, induced antibody (Ab) response, CD8 lymphocytes with cytotoxic function, and detectible binding of the cognate peptide epitopes for human HLA A*0201 restriction using tetramer technology.
Gallez-Hawkins
Ghislaine
G
Department of Virology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
Li
Xiuli
X
Franck
Anne E
AE
Thao
Lia
L
Lacey
Simon F
SF
Diamond
Don J
DJ
Zaia
John A
JA
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D003587:Cytomegalovirus / Q000276:immunology; D022482:Cytomegalovirus Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000229:Dependovirus / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D017874:Immediate-Early Proteins / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D022482:Cytomegalovirus Vaccines; D006680:HLA Antigens; C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D014614:Vaccines, Synthetic; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C065418:cytomegalovirus matrix protein 65kDa