Molecular detection of linezolid resistance in Enterococcus faecium and Enterococcus faecalis by use of 5' nuclease real-time PCR compared to a modified classical approach.
A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.
Werner
Guido
G
Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. wernerg@rki.de
Strommenger
Birgit
B
Klare
Ingo
I
Witte
Wolfgang
W
D000081:Acetamides / Q000494:pharmacology*; D000483:Alleles; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects*; D016984:Enterococcus faecium / Q000187:drug effects*; D006801:Humans; D000069349:Linezolid; D020411:Oligonucleotide Array Sequence Analysis; D023303:Oxazolidinones / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D019914:Taq Polymerase
D000081:Acetamides; D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D019914:Taq Polymerase; D000069349:Linezolid