HPLC profiling and quantification of active principles in leaves of Hedera helix L.
Ivy (Hedera helix L., Araliaceae), is an evergreen medicinal and ornamental plant. Depending on leaf polymorphism different shaped ivy leaves were extracted and subsequently analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). Quantitative determination of its most prominent saponins hederacoside C (1) and alpha-hederin (2) from different ivy leaf extracts were detected, validated and optimized for quick profiling. The linearity of response, repeatability and reproducibility of the applied RP-HPLC method are reported.
Demirci
B
B
Department of Pharmaceutical Biology, Faculty of Chemistry and Pharmacy, University of Regensburg, Regensburg, Germany.
Goppel
M
M
Demirci
F
F
Franz
G
G
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D035981:Hedera / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
D010936:Plant Extracts; D012503:Saponins; C482302:hederacoside C; D009828:Oleanolic Acid