Peptide YY inhibits the growth of Barrett's esophageal adenocarcinoma in vitro.
BACKGROUND
Peptide YY (PYY) is an endogenous gut hormone that inhibits the growth of certain cancers. Adenocarcinoma of the esophagus usually arises from Barrett's esophagus. We hypothesized that treatment of Barrett's adenocarcinoma with PYY would result in decreased proliferation.


METHODS
Barrett's cancer cell lines (BIC and SEG-1) were treated with PYY (3-36) at 500 pmol/mL. Viability was measured by MTT at 24 and 72 hours. Apoptosis and necrosis was evaluated by flow cytometry.


RESULTS
PYY reduced proliferation in SEG-1 cells at 24 hours (21.2% +/- 3.4%, P <0.001) and 72 hours (14.2% +/- 6.2%, P <0.001). In the BIC cells, growth was inhibited by 7.9% +/- 7.0%, P = 0.021 after 72 hours. PYY increased late apoptotic activity in SEG-1 cells by 31%, P = 0.014.


CONCLUSIONS
This is the first report of antiproliferative effects of PYY against Barrett's carcinoma in vitro. Reductions in cell growth appear to be mediated by proapoptotic mechanisms. Further investigation of PYY in the treatment of Barrett's adenocarcinoma is warranted.
McFadden
David W
DW
Department of Surgery, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, USA. dmcfadden@hsc.wvu.edu
Riggs
Dale R
DR
Jackson
Barbara J
BJ
Vona-Davis
Linda
L
D000230:Adenocarcinoma / Q000473:pathology*; D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects*; D001471:Barrett Esophagus / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D019894:Peptide YY / Q000494:pharmacology*; D011336:Probability; D012307:Risk Factors; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D019894:Peptide YY