Preparation and characterization of long methacrylate monolithic column for capillary liquid chromatography.
Long methacrylate monolithic columns (100 cm x 320 microm i.d.) were prepared from silanized fused-silica capillaries of 320 microm i.d. by in situ copolymerization of butyl methacrylate (BMA) with ethylene dimethacrylate (EDMA) in the presence of a suitable porogen. The separation performance and selectivity of the column were evaluated and compared with a 25 cm x 320 microm i.d. column prepared in the same way by capillary high-performance liquid chromatography (micro-HPLC). The results showed that the 1 m long monolithic column can generate 33 x 10(3) plate number and exhibited good permeability, higher sample loadability, and separation capability.
Shu
Xin
X
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Chen
Lingxin
L
Yang
Bingcheng
B
Guan
Yafeng
Y
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010539:Permeability
D008689:Methacrylates