Determination of rosiglitazone in coated tablets by MEKC and HPLC methods.
Micellar electrokinetic chromatographic (MEKC) and high-performance liquid chromatographic (HPLC) methods were developed and subsequently validated for the determination of rosiglitazone (RSG) in coated tablet, a potent new oral antihyperglicemic agent. The electrophoretic separation was performed in a fused-silica capillary of total length 48.0 cm (effective length 39.5 cm, 75 microm i.d.) using 10 mM sodium tetraborate buffer (pH 9.0) containing 30 mM sodium dodecyl sulfate (SDS) as the background electrolyte (BGE). The separating voltage used was of 20 kV at 25 degrees C and the diode array detector was set at 247 nm. The MEKC method was compared with HPLC method using a RP-18 column (125 x 4.0mm i.d.) eluted with a mobile phase consisting of mixture of 25 mM potassium dihydrogen phosphate buffer and acetonitrile (55:45, v/v), adjusting the pH to 6.2 with dilute potassium hydroxide. Statistical analysis by Student's t-test showed no significant differences between the results obtained by two methods. The results indicated that MEKC can be used an alternative method to HPLC for the determination of rosiglitazone in pharmaceutical dosage form.
Gomes
Patrícia
P
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 Lab.402, Porto Alegre 90610-000, RS, Brazil. patriciagomes0@yahoo.com.br
Sippel
Juliana
J
Jablonski
André
A
Steppe
Martin
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D000077154:Rosiglitazone; D013608:Tablets, Enteric-Coated; D045162:Thiazolidinediones / Q000032:analysis* / Q000737:chemistry
D013608:Tablets, Enteric-Coated; D045162:Thiazolidinediones; D000077154:Rosiglitazone